MX2023000450A - Glutamato deshidrogenasa modificada y uso de la misma. - Google Patents

Glutamato deshidrogenasa modificada y uso de la misma.

Info

Publication number
MX2023000450A
MX2023000450A MX2023000450A MX2023000450A MX2023000450A MX 2023000450 A MX2023000450 A MX 2023000450A MX 2023000450 A MX2023000450 A MX 2023000450A MX 2023000450 A MX2023000450 A MX 2023000450A MX 2023000450 A MX2023000450 A MX 2023000450A
Authority
MX
Mexico
Prior art keywords
modified
gludh
glutamate dehydrogenase
glufosinate
ammonium
Prior art date
Application number
MX2023000450A
Other languages
English (en)
Inventor
Wei Xu
Xinkai Xie
Junying Fan
Original Assignee
Hunan Lier Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hunan Lier Biotech Co Ltd filed Critical Hunan Lier Biotech Co Ltd
Publication of MX2023000450A publication Critical patent/MX2023000450A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)

Abstract

La presente invención se refiere a una glutamato deshidrogenasa (GluDH) modificada. En particular la GluDH modificada de la presente invención tiene has una aumentada actividad para catalizar la reacción de ácido 4-(hidroximetilfosfinil)-2-oxobutan oico (PPO) y un dador de amino para generar L-glufosinato y/o una propiedad dinámica mejorada. La presente invención también se refiere al polinucleótido que codifica la GluDH modificada de la presente invención, el vector y la célula huésped para expresar la GluDH modificada de la presente invención y el método para producir L-glufosinato con la GluDH modificada y la célula huésped de la presente invención.
MX2023000450A 2020-07-09 2021-07-08 Glutamato deshidrogenasa modificada y uso de la misma. MX2023000450A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010659106 2020-07-09
PCT/CN2021/105179 WO2022007881A1 (zh) 2020-07-09 2021-07-08 经修饰的谷氨酸脱氢酶及其应用

Publications (1)

Publication Number Publication Date
MX2023000450A true MX2023000450A (es) 2023-04-19

Family

ID=79232923

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023000450A MX2023000450A (es) 2020-07-09 2021-07-08 Glutamato deshidrogenasa modificada y uso de la misma.

Country Status (13)

Country Link
US (1) US20230332113A1 (es)
EP (1) EP4180524A1 (es)
JP (1) JP2023532699A (es)
KR (1) KR20230031956A (es)
CN (1) CN113913401A (es)
AR (1) AR122916A1 (es)
AU (1) AU2021306531A1 (es)
BR (1) BR112023000234A2 (es)
CA (1) CA3184703A1 (es)
IL (1) IL299379A (es)
MX (1) MX2023000450A (es)
TW (1) TW202208620A (es)
WO (1) WO2022007881A1 (es)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006015885A1 (en) * 2004-08-13 2006-02-16 University College Dublin, National University Of Ireland, Dublin Amino acid dehydrogenase-derived biocatalysts
EP3201326B1 (en) * 2014-10-03 2020-03-04 Metabolic Explorer Mutant glutamate dehydrogenase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
CN105567780A (zh) 2016-01-14 2016-05-11 重庆惠健生物科技有限公司 一种l-草铵膦的酶-化学催化去消旋化制备方法
CN109072261B (zh) 2016-03-02 2023-03-17 巴斯夫欧洲公司 制造l-草胺膦的方法
CN107630052B (zh) 2017-03-29 2018-05-29 武汉茵茂特生物技术有限公司 L-草铵膦的生物转化方法
CN106978453B (zh) 2017-03-31 2019-10-29 浙江大学 一种利用氨基酸脱氢酶制备l-草铵膦的方法
CN108588045B (zh) 2018-03-09 2019-12-10 浙江大学 谷氨酸脱氢酶突变体及其在制备l-草铵膦中的应用
CN108690854B (zh) 2018-04-16 2022-03-18 浙江工业大学 一种利用化学-酶法生产l-草铵膦的方法
CN109609474B (zh) 2018-12-28 2020-07-28 浙江工业大学 一种氨基酸脱氢酶突变体及其在合成l-草铵膦中的应用
CN111057687B (zh) * 2019-05-23 2020-10-27 弈柯莱生物科技(上海)股份有限公司 一种l-谷氨酸脱氢酶突变体及其应用

Also Published As

Publication number Publication date
EP4180524A1 (en) 2023-05-17
CA3184703A1 (en) 2022-01-13
US20230332113A1 (en) 2023-10-19
AR122916A1 (es) 2022-10-12
AU2021306531A1 (en) 2023-03-02
TW202208620A (zh) 2022-03-01
CN113913401A (zh) 2022-01-11
IL299379A (en) 2023-02-01
BR112023000234A2 (pt) 2023-03-14
KR20230031956A (ko) 2023-03-07
WO2022007881A1 (zh) 2022-01-13
JP2023532699A (ja) 2023-07-31

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