BR112023000234A2 - Glutamato desidrogenase modificada e uso do mesmo - Google Patents

Glutamato desidrogenase modificada e uso do mesmo

Info

Publication number
BR112023000234A2
BR112023000234A2 BR112023000234A BR112023000234A BR112023000234A2 BR 112023000234 A2 BR112023000234 A2 BR 112023000234A2 BR 112023000234 A BR112023000234 A BR 112023000234A BR 112023000234 A BR112023000234 A BR 112023000234A BR 112023000234 A2 BR112023000234 A2 BR 112023000234A2
Authority
BR
Brazil
Prior art keywords
modified
present
gludh
glutamate dehydrogenase
modified glutamate
Prior art date
Application number
BR112023000234A
Other languages
English (en)
Inventor
Xie Xinkai
Xu Wei
Fan Junying
Original Assignee
Hunan Lier Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hunan Lier Biotech Co Ltd filed Critical Hunan Lier Biotech Co Ltd
Publication of BR112023000234A2 publication Critical patent/BR112023000234A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

GLUTAMATO DESIDROGENASE MODIFICADA E USO DO MESMO. A presente invenção refere-se a uma glutamato desidrogenase modificada (GluDH). Em particular, a GluDH modificada da presente invenção tem uma atividade aumentada para catalisar a reação do ácido 4-(hidroximetilfosfinil)-2-oxobutanoico (PPO) e um doador de amino para gerar L-glufosinato e/ou uma propriedade dinâmica melhorada. A presente invenção também se refere ao polinucleotídeo que codifica a GluDH modificada da presente invenção, o vetor e a célula hospedeira para expressar a GluDH modificada da presente invenção e o método de produção de L-glufosinato com a GluDH modificada e a célula hospedeira da presente invenção.
BR112023000234A 2020-07-09 2021-07-08 Glutamato desidrogenase modificada e uso do mesmo BR112023000234A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010659106 2020-07-09
PCT/CN2021/105179 WO2022007881A1 (zh) 2020-07-09 2021-07-08 经修饰的谷氨酸脱氢酶及其应用

Publications (1)

Publication Number Publication Date
BR112023000234A2 true BR112023000234A2 (pt) 2023-03-14

Family

ID=79232923

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023000234A BR112023000234A2 (pt) 2020-07-09 2021-07-08 Glutamato desidrogenase modificada e uso do mesmo

Country Status (13)

Country Link
US (1) US20230332113A1 (pt)
EP (1) EP4180524A1 (pt)
JP (1) JP2023532699A (pt)
KR (1) KR20230031956A (pt)
CN (1) CN113913401A (pt)
AR (1) AR122916A1 (pt)
AU (1) AU2021306531A1 (pt)
BR (1) BR112023000234A2 (pt)
CA (1) CA3184703A1 (pt)
IL (1) IL299379A (pt)
MX (1) MX2023000450A (pt)
TW (1) TW202208620A (pt)
WO (1) WO2022007881A1 (pt)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006015885A1 (en) * 2004-08-13 2006-02-16 University College Dublin, National University Of Ireland, Dublin Amino acid dehydrogenase-derived biocatalysts
WO2016050959A2 (en) * 2014-10-03 2016-04-07 Metabolic Explorer Mutant glutamate dehydrogenase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
CN105567780A (zh) 2016-01-14 2016-05-11 重庆惠健生物科技有限公司 一种l-草铵膦的酶-化学催化去消旋化制备方法
MX2018010425A (es) 2016-03-02 2018-11-29 Agrimetis Llc Metodos de preparacion de l-glufosinato.
CN107630052B (zh) 2017-03-29 2018-05-29 武汉茵茂特生物技术有限公司 L-草铵膦的生物转化方法
CN106978453B (zh) 2017-03-31 2019-10-29 浙江大学 一种利用氨基酸脱氢酶制备l-草铵膦的方法
CN108588045B (zh) 2018-03-09 2019-12-10 浙江大学 谷氨酸脱氢酶突变体及其在制备l-草铵膦中的应用
CN108690854B (zh) 2018-04-16 2022-03-18 浙江工业大学 一种利用化学-酶法生产l-草铵膦的方法
CN109609474B (zh) 2018-12-28 2020-07-28 浙江工业大学 一种氨基酸脱氢酶突变体及其在合成l-草铵膦中的应用
CN111057687B (zh) * 2019-05-23 2020-10-27 弈柯莱生物科技(上海)股份有限公司 一种l-谷氨酸脱氢酶突变体及其应用

Also Published As

Publication number Publication date
KR20230031956A (ko) 2023-03-07
AU2021306531A1 (en) 2023-03-02
CA3184703A1 (en) 2022-01-13
WO2022007881A1 (zh) 2022-01-13
TW202208620A (zh) 2022-03-01
JP2023532699A (ja) 2023-07-31
US20230332113A1 (en) 2023-10-19
MX2023000450A (es) 2023-04-19
CN113913401A (zh) 2022-01-11
EP4180524A1 (en) 2023-05-17
AR122916A1 (es) 2022-10-12
IL299379A (en) 2023-02-01

Similar Documents

Publication Publication Date Title
Nestl et al. Engineering of flexible loops in enzymes
Dannheim et al. Clostridioides difficile 630Δerm in silico and in vivo–quantitative growth and extensive polysaccharide secretion
BR112016000745A2 (pt) Processo para a preparação de rebaudiosídeo m
BR112012005737A8 (pt) Método para a produção de ácido 3-hidróxi-3-metilbutírico a partir de acetona e um composto acetila ativado.
BR112021016818A2 (pt) Método para síntese in vivo de 4-hidroximetilfurfural e derivados do mesmo
BR112015001601A2 (pt) células de levedura tendo curso de tca redutor de piruvato em succinato e superexpressão de uma enzima transidrogenase nad(p)+ exógena
Pain et al. Mitochondrial NADH kinase, Pos5p, is required for efficient iron-sulfur cluster biogenesis in Saccharomyces cerevisiae
JP2014236749A5 (pt)
BR112014031942A2 (pt) enzimas e métodos para síntese de estireno
BR112017026596A2 (pt) microrganismo que produz ácido lático e método para produção de ácido lático utilizando o mesmo
Ferry Acetate kinase and phosphotransacetylase
BR112017012416A2 (pt) fenilpiruvato descarboxilase geneticamente modificada, processos para preparar e usos da mesma
BR112021021601A2 (pt) Microrganismo geneticamente modificado e método de produção de ácido 3-hidroxiadípico, ácido a-hidromucônico e/ou ácido adípico
BR112023000234A2 (pt) Glutamato desidrogenase modificada e uso do mesmo
BR112016017092A2 (pt) Bactéria acetogênica carboxidotrófica, e, método para produzir um produto
Cai et al. Metabolic regulation adapting to high methanol environment in the methylotrophic yeast Ogataea methanolica
MX2022010514A (es) Metodos de secuenciacion de biopolimeros.
BR112022010266A2 (pt) Daao modificado e uso do mesmo
BR112023014906A2 (pt) Molécula de ácido nucleico funcional e método
EA201892627A1 (ru) Микроорганизм с модифицированной активностью альдегид:ферредоксин оксидоредуктазы и связанные способы
AR128162A1 (es) Glutamato deshidrogenasa modificada y uso de la misma
BR112018016927A2 (pt) processos e materiais biocatalíticos para utilização de carbono aprimorada
BR112023005370A2 (pt) Composições e métodos para aumentar função das células-tronco
BR112021026484A2 (pt) Polimerização de enzimas para reações de sequenciamento
WO2021236763A3 (en) dsRNA DIRECTED TO CORONAVIRUS PROTEINS