BR112022010266A2 - Daao modificado e uso do mesmo - Google Patents

Daao modificado e uso do mesmo

Info

Publication number
BR112022010266A2
BR112022010266A2 BR112022010266A BR112022010266A BR112022010266A2 BR 112022010266 A2 BR112022010266 A2 BR 112022010266A2 BR 112022010266 A BR112022010266 A BR 112022010266A BR 112022010266 A BR112022010266 A BR 112022010266A BR 112022010266 A2 BR112022010266 A2 BR 112022010266A2
Authority
BR
Brazil
Prior art keywords
daao
modified
present
glufosinate
ppo
Prior art date
Application number
BR112022010266A
Other languages
English (en)
Inventor
Xie Xinkai
Xu Wei
Fan Junying
Original Assignee
Hunan Lier Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hunan Lier Biotechnology Co Ltd filed Critical Hunan Lier Biotechnology Co Ltd
Publication of BR112022010266A2 publication Critical patent/BR112022010266A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12N9/0024—D-Amino acid oxidase (1.4.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/002—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by oxidation/reduction reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03003—D-Amino-acid oxidase (1.4.3.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

DAAO MODIFICADO E USO DO MESMO. A presente invenção refere-se a um D-aminoácido oxidase (DAAO) mo-dificado. Em particular, o DAAO modificado da presente invenção tem a atividade de catalisar a oxidação de D-glufosinato em PPO. Ainda, o DAAO modificado da presente invenção aumentou a atividade de catali-sar a oxidação de D-glufosinato em PPO e/ou aumentou a estabilidade conforme comparado com SEQ ID NO: 4. A presente invenção ainda se refere ao polinucleotídeo que codifica o DAAO modificado da presente invenção, o vetor e a célula hospedeira expressando o DAAO modificado da presente invenção, e o método para produzir L-glufosinato com o DAAO modificado e a célula hospedeira da presente invenção.
BR112022010266A 2019-12-09 2020-12-08 Daao modificado e uso do mesmo BR112022010266A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201911249924 2019-12-09
PCT/CN2020/134540 WO2021115256A1 (zh) 2019-12-09 2020-12-08 经修饰的daao酶及其应用

Publications (1)

Publication Number Publication Date
BR112022010266A2 true BR112022010266A2 (pt) 2022-10-04

Family

ID=76328838

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022010266A BR112022010266A2 (pt) 2019-12-09 2020-12-08 Daao modificado e uso do mesmo

Country Status (13)

Country Link
US (2) US11667896B2 (pt)
EP (1) EP4074837A4 (pt)
JP (1) JP2023504869A (pt)
KR (1) KR20220136350A (pt)
CN (2) CN113825841B (pt)
AR (1) AR120690A1 (pt)
AU (1) AU2020401155A1 (pt)
BR (1) BR112022010266A2 (pt)
CA (1) CA3160673A1 (pt)
IL (1) IL293679A (pt)
MX (1) MX2022006534A (pt)
TW (1) TWI777324B (pt)
WO (1) WO2021115256A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113881582A (zh) * 2021-11-11 2022-01-04 合肥工业大学 一株去除重金属离子的红酵母mf4、菌剂及其应用
CN116064446B (zh) * 2022-12-02 2023-11-14 湖南麦肯伟科技有限公司 一种d-氨基酸氧化酶的突变体、表达载体、基因工程菌及其构建方法和应用
CN116200356A (zh) * 2022-12-05 2023-06-02 浙江工业大学 D-氨基酸氧化酶突变体及其在制备l-草铵膦中的应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7939709B2 (en) * 2002-02-26 2011-05-10 Syngenta Limited Method for selectively producing male or female sterile plants
EP3277822B1 (en) * 2015-04-03 2020-05-06 Temasek Life Sciences Laboratory Limited D-amino acid-inducible gene expression system for rhodosporidium and rhodotorula
CN105567780A (zh) 2016-01-14 2016-05-11 重庆惠健生物科技有限公司 一种l-草铵膦的酶-化学催化去消旋化制备方法
CN116121316A (zh) * 2016-03-02 2023-05-16 巴斯夫欧洲公司 制造l-草胺膦的方法
CN107502647B (zh) 2017-09-15 2020-12-15 浙江大学 一种生物酶法去消旋化制备l-草铵膦的方法
CN108690854B (zh) 2018-04-16 2022-03-18 浙江工业大学 一种利用化学-酶法生产l-草铵膦的方法
EP3847269A1 (en) * 2018-09-05 2021-07-14 Basf Se Methods for improving yields of l-glufosinate
CN111019916B (zh) * 2018-11-23 2020-12-08 弈柯莱生物科技(上海)股份有限公司 一种d-氨基酸氧化酶突变体及其应用
CN109576236B (zh) * 2018-12-28 2019-12-17 浙江工业大学 一种d-氨基酸氧化酶突变体及其应用
CN109609582B (zh) * 2019-01-17 2021-11-09 重庆惠健生物科技有限公司 一种微生物催化去消旋化制备l-草铵膦的方法

Also Published As

Publication number Publication date
KR20220136350A (ko) 2022-10-07
WO2021115256A1 (zh) 2021-06-17
JP2023504869A (ja) 2023-02-07
AU2020401155A1 (en) 2022-06-30
MX2022006534A (es) 2022-09-07
IL293679A (en) 2022-08-01
TWI777324B (zh) 2022-09-11
EP4074837A1 (en) 2022-10-19
TW202128992A (zh) 2021-08-01
US20220333086A1 (en) 2022-10-20
CN116286699A (zh) 2023-06-23
EP4074837A4 (en) 2023-12-27
CN113825841A (zh) 2021-12-21
CN113825841B (zh) 2022-08-09
US20230416696A1 (en) 2023-12-28
US11667896B2 (en) 2023-06-06
CA3160673A1 (en) 2021-06-17
AR120690A1 (es) 2022-03-09

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Owner name: HUNAN LIER BIOTECH CO., LTD. (CN)