CN113825841A - 经修饰的daao酶及其应用 - Google Patents

经修饰的daao酶及其应用 Download PDF

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CN113825841A
CN113825841A CN202080032605.2A CN202080032605A CN113825841A CN 113825841 A CN113825841 A CN 113825841A CN 202080032605 A CN202080032605 A CN 202080032605A CN 113825841 A CN113825841 A CN 113825841A
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substitution
daao
modified
amino acid
positions
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CN113825841B (zh
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谢新开
徐伟
范俊英
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Sichuan Lier Biotechnology Co ltd
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Sichuan Lier Biotechnology Co ltd
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    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12N9/0024—D-Amino acid oxidase (1.4.3.3)
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    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
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    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
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    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03003—D-Amino-acid oxidase (1.4.3.3)

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Abstract

本发明涉及经修饰的D‑氨基酸氧化酶(DAAO)。具体而言，本发明的经修饰的DAAO具有催化D草铵膦氧化为PPO的活性。此外，本发明的经修饰的DAAO与SEQ ID NO:4相比具有提高的催化D草铵膦氧化为PPO的活性和/或提高的稳定性。本发明还涉及编码本发明的经修饰的DAAO的多核苷酸、表达本发明的经修饰的DAAO的载体和宿主细胞以及使用本发明的经修饰的DAAO和宿主细胞生产L‑草铵膦的方法。

Description

PCT国内申请，说明书已公开。

Claims (16)

  1. PCT国内申请，权利要求书已公开。
CN202080032605.2A 2019-12-09 2020-12-08 经修饰的daao酶及其应用 Active CN113825841B (zh)

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CN201911249924 2019-12-09
CN2019112499244 2019-12-09
PCT/CN2020/134540 WO2021115256A1 (zh) 2019-12-09 2020-12-08 经修饰的daao酶及其应用

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EP (1) EP4074837A4 (zh)
JP (1) JP2023504869A (zh)
KR (1) KR20220136350A (zh)
CN (2) CN116286699A (zh)
AR (1) AR120690A1 (zh)
AU (1) AU2020401155A1 (zh)
BR (1) BR112022010266A2 (zh)
CA (1) CA3160673A1 (zh)
IL (1) IL293679A (zh)
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113881582A (zh) * 2021-11-11 2022-01-04 合肥工业大学 一株去除重金属离子的红酵母mf4、菌剂及其应用
CN116064446A (zh) * 2022-12-02 2023-05-05 中南大学 一种d-氨基酸氧化酶的突变体、表达载体、基因工程菌及其构建方法和应用

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CN1639344A (zh) * 2002-02-26 2005-07-13 辛根塔有限公司 选择性产生雄性或雌性不育植物的方法
CN109072261A (zh) * 2016-03-02 2018-12-21 阿格里麦蒂斯有限责任公司 制造l-草胺膦的方法
CN109576236A (zh) * 2018-12-28 2019-04-05 浙江工业大学 一种d-氨基酸氧化酶突变体及其应用
WO2020051188A1 (en) * 2018-09-05 2020-03-12 Agrimetis, Llc Methods for improving yields of l-glufosinate
CN111019916A (zh) * 2018-11-23 2020-04-17 上海弈柯莱生物医药科技有限公司 一种d-氨基酸氧化酶突变体及其应用

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EP3277822B1 (en) * 2015-04-03 2020-05-06 Temasek Life Sciences Laboratory Limited D-amino acid-inducible gene expression system for rhodosporidium and rhodotorula
CN105567780A (zh) 2016-01-14 2016-05-11 重庆惠健生物科技有限公司 一种l-草铵膦的酶-化学催化去消旋化制备方法
CN107502647B (zh) 2017-09-15 2020-12-15 浙江大学 一种生物酶法去消旋化制备l-草铵膦的方法
CN108690854B (zh) 2018-04-16 2022-03-18 浙江工业大学 一种利用化学-酶法生产l-草铵膦的方法
CN109609582B (zh) 2019-01-17 2021-11-09 重庆惠健生物科技有限公司 一种微生物催化去消旋化制备l-草铵膦的方法

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CN1639344A (zh) * 2002-02-26 2005-07-13 辛根塔有限公司 选择性产生雄性或雌性不育植物的方法
CN109072261A (zh) * 2016-03-02 2018-12-21 阿格里麦蒂斯有限责任公司 制造l-草胺膦的方法
US20190185889A1 (en) * 2016-03-02 2019-06-20 Agrimetis, Llc Methods for Making L-Glufosinate
WO2020051188A1 (en) * 2018-09-05 2020-03-12 Agrimetis, Llc Methods for improving yields of l-glufosinate
CN111019916A (zh) * 2018-11-23 2020-04-17 上海弈柯莱生物医药科技有限公司 一种d-氨基酸氧化酶突变体及其应用
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113881582A (zh) * 2021-11-11 2022-01-04 合肥工业大学 一株去除重金属离子的红酵母mf4、菌剂及其应用
CN116064446A (zh) * 2022-12-02 2023-05-05 中南大学 一种d-氨基酸氧化酶的突变体、表达载体、基因工程菌及其构建方法和应用
CN116064446B (zh) * 2022-12-02 2023-11-14 湖南麦肯伟科技有限公司 一种d-氨基酸氧化酶的突变体、表达载体、基因工程菌及其构建方法和应用

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EP4074837A4 (en) 2023-12-27
AR120690A1 (es) 2022-03-09
CA3160673A1 (en) 2021-06-17
MX2022006534A (es) 2022-09-07
US20230416696A1 (en) 2023-12-28
EP4074837A1 (en) 2022-10-19
WO2021115256A1 (zh) 2021-06-17
AU2020401155A1 (en) 2022-06-30
KR20220136350A (ko) 2022-10-07
CN116286699A (zh) 2023-06-23
JP2023504869A (ja) 2023-02-07
IL293679A (en) 2022-08-01
US11667896B2 (en) 2023-06-06
TWI777324B (zh) 2022-09-11
CN113825841B (zh) 2022-08-09
BR112022010266A2 (pt) 2022-10-04
TW202128992A (zh) 2021-08-01
US20220333086A1 (en) 2022-10-20

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