AR110093A1 - D-psicosa 3-epimerasa y método para preparar d-psicosa utilizando la misma - Google Patents

D-psicosa 3-epimerasa y método para preparar d-psicosa utilizando la misma

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Publication number
AR110093A1
AR110093A1 ARP170103190A ARP170103190A AR110093A1 AR 110093 A1 AR110093 A1 AR 110093A1 AR P170103190 A ARP170103190 A AR P170103190A AR P170103190 A ARP170103190 A AR P170103190A AR 110093 A1 AR110093 A1 AR 110093A1
Authority
AR
Argentina
Prior art keywords
epimerase
psychosa
psychose
microorganism
prepare
Prior art date
Application number
ARP170103190A
Other languages
English (en)
Inventor
Seong Jun Cho
Seung Won Park
Seong Bo Kim
Yang Hee Kim
Young Mi Lee
Su Jin Kim
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of AR110093A1 publication Critical patent/AR110093A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/24Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y501/00Racemaces and epimerases (5.1)
    • C12Y501/03Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Reivindicación 1: Una D-psicosa 3-epimerasa que consiste en la secuencia de aminoácidos de SEQ ID Nº 1. Reivindicación 3: Un polinucleótido que codifica la D-psicosa 3-epimerasa de la reivindicación 1. Reivindicación 5: Un microorganismo en el que se introduce el vector recombinante de la reivindicación 4. Reivindicación 6: Una composición para preparar D-psicosa que comprende: la D-psicosa 3-epimerasa de la reivindicación 1, un microorganismo que expresa la D-psicosa 3-epimerasa, o un cultivo del microorganismo. Reivindicación 8: Un método para preparar D-psicosa que comprende: contactar la D-psicosa 3-epimerasa de la reivindicación 1, un microorganismo que expresa la D-psicosa 3-epimerasa, o un cultivo del microorganismo con D-fructosa.
ARP170103190A 2016-11-16 2017-11-16 D-psicosa 3-epimerasa y método para preparar d-psicosa utilizando la misma AR110093A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20160152947 2016-11-16

Publications (1)

Publication Number Publication Date
AR110093A1 true AR110093A1 (es) 2019-02-20

Family

ID=62145632

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP170103190A AR110093A1 (es) 2016-11-16 2017-11-16 D-psicosa 3-epimerasa y método para preparar d-psicosa utilizando la misma

Country Status (10)

Country Link
US (1) US11174475B2 (es)
EP (1) EP3543336A4 (es)
JP (1) JP6967599B2 (es)
KR (1) KR101919713B1 (es)
CN (1) CN110462036B (es)
AR (1) AR110093A1 (es)
RU (1) RU2727903C1 (es)
TW (1) TWI666323B (es)
UA (1) UA126473C2 (es)
WO (1) WO2018093153A1 (es)

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KR101965509B1 (ko) * 2017-11-15 2019-04-03 씨제이제일제당 (주) 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법
CN110904132B (zh) * 2019-11-05 2022-08-16 吉林中粮生化有限公司 D-阿洛酮糖3-差向异构酶的编码基因、载体、重组细胞以及它们的应用
CN110862980B (zh) * 2019-11-29 2021-05-04 浙江工业大学 一种d-阿洛酮糖3-差向异构酶突变体及其应用
CN111019928B (zh) * 2019-12-11 2022-08-16 吉林中粮生化有限公司 D-阿洛酮糖3-差向异构酶的编码基因、载体、重组细胞以及它们的应用
KR102254411B1 (ko) * 2019-12-19 2021-05-24 대상 주식회사 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법
KR102682846B1 (ko) * 2021-11-19 2024-07-08 대상 주식회사 열 안정성이 우수한 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법
EP4257689A4 (en) 2021-12-29 2024-09-11 Daesang Corp NEW PROMOTER VARIANT FOR CONSTITUTIVE EXPRESSION AND ITS USE
CN114262703A (zh) * 2021-12-31 2022-04-01 保龄宝生物股份有限公司 一种利用膜富集d-阿洛酮糖3-差向异构酶的方法及应用
KR102691596B1 (ko) 2022-12-20 2024-08-06 대상 주식회사 항시발현용 신규 프로모터 변이체 및 이의 용도

Family Cites Families (15)

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EP1956088B1 (en) * 2005-11-15 2013-10-23 Hayashibara Co., Ltd. Ketose 3-epimerase, process for production thereof, and use thereof
GB0822937D0 (en) * 2008-12-16 2009-01-21 Terranol As Microorganism
KR20110035805A (ko) 2009-09-30 2011-04-06 씨제이제일제당 (주) 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법
KR101203856B1 (ko) * 2011-08-24 2012-11-21 씨제이제일제당 (주) 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산
CN104769125B (zh) * 2012-08-10 2018-10-23 株式会社三养社 阿洛酮糖差向异构酶及使用其的用于转变为阿洛酮糖的组合物
CN108315316B (zh) * 2013-01-08 2021-07-20 松谷化学工业株式会社 球形节杆菌生产的酮糖3-差向异构酶
KR101455624B1 (ko) * 2013-04-12 2014-10-28 주식회사한국야쿠르트 기능성 희귀당 사이코스의 생산능을 지닌 신규 클로스트리디움 볼티에 유래의 사이코스-3-에피머화 효소 및 이를 이용한 사이코스 생산방법
KR101455759B1 (ko) * 2013-04-23 2014-10-28 씨제이제일제당(주) 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 제조 방법
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KR101539097B1 (ko) * 2013-12-26 2015-07-23 주식회사 삼양제넥스 사이코스 에피머화 효소를 암호화 하는 폴리뉴클레오타이드, 및 이를 이용하는 사이코스 생산방법
CN103710330B (zh) * 2014-01-03 2015-04-22 江南大学 一种高催化活性的d-阿洛酮糖 3-差向异构酶的突变体酶及其应用
CN103849613A (zh) * 2014-01-03 2014-06-11 江南大学 一种热稳定性提高的d-阿洛酮糖 3-差向异构酶的突变体酶及其应用
US10550414B2 (en) * 2015-12-23 2020-02-04 Cj Cheiljedang Corporation Composition for producing D-psicose comprising D-psicose 3-epimerase and salt and method for producing D-psicose using same
CN105602879B (zh) * 2016-01-26 2019-09-03 中国科学院天津工业生物技术研究所 一株高效分泌d-阿洛酮糖 3-差向异构酶的基因工程菌株、构建方法及其应用
CN105802897B (zh) * 2016-05-26 2019-02-12 江南大学 一种d-阿洛酮糖-3-差向异构酶生产菌株及其应用

Also Published As

Publication number Publication date
US20200063117A1 (en) 2020-02-27
CN110462036A (zh) 2019-11-15
TWI666323B (zh) 2019-07-21
JP2019535320A (ja) 2019-12-12
WO2018093153A1 (ko) 2018-05-24
JP6967599B2 (ja) 2021-11-17
KR101919713B1 (ko) 2018-11-19
KR20180055736A (ko) 2018-05-25
US11174475B2 (en) 2021-11-16
EP3543336A4 (en) 2020-06-17
RU2727903C1 (ru) 2020-07-24
EP3543336A1 (en) 2019-09-25
TW201823456A (zh) 2018-07-01
CN110462036B (zh) 2023-07-14
UA126473C2 (uk) 2022-10-12

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