AR093923A1 - Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codifican - Google Patents
Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codificanInfo
- Publication number
- AR093923A1 AR093923A1 ARP130104633A ARP130104633A AR093923A1 AR 093923 A1 AR093923 A1 AR 093923A1 AR P130104633 A ARP130104633 A AR P130104633A AR P130104633 A ARP130104633 A AR P130104633A AR 093923 A1 AR093923 A1 AR 093923A1
- Authority
- AR
- Argentina
- Prior art keywords
- polypeptide
- phospholipase
- activity
- polypeptides
- seq
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
- A23D9/013—Other fatty acid esters, e.g. phosphatides
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/04—Refining fats or fatty oils by chemical reaction with acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/04—Phosphoric diester hydrolases (3.1.4)
- C12Y301/04003—Phospholipase C (3.1.4.3)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Edible Oils And Fats (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
Abstract
La presente se relaciona con polipéptidos aislados que tienen actividad de fosfolipasa C, y polinucleótidos que codifican a los polipéptidos. La solicitud también se relaciona con constructos de ácidos nucleicos, vectores y células huésped que comprenden los polinucleótidos como así también métodos para producir y utilizar los polipéptidos. Reivindicación 1: Un polipéptido aislado que tiene actividad de fosfolipasa C, seleccionado del grupo que consiste en: a) un polipéptido que tiene al menos 90%, al menos 91%, al menos 92%, al menos 93%, al menos 94%, al menos 95%, al menos 96%, al menos 97%, al menos 98%, al menos 99% o 100% de identidad de secuencia con el polipéptido maduro de la SEC ID Nº 2; b) un polipéptido codificado por un polinucleótido que se hibrida en condiciones muy restrictivas con i) el polipéptido maduro que codifica la secuencia SEC ID Nº 1, o ii) el complemento de longitud completa de i); c) un polipéptido codificado por un polinucleótido que tiene al menos 90%, al menos 91%, al menos 92%, al menos 93%, al menos 94%, al menos 95%, al menos 96%, al menos 97%, al menos 98%, al menos 99% o 100% de identidad de secuencia con el polipéptido maduro de la SEC ID Nº 1; d) una variante del polipéptido maduro de la SEC ID Nº 2 que comprende una sustitución, supresión y/o inserción en una o más posiciones; y e) un fragmento del polipéptido de a), b), c) o d) que tiene actividad de fosfolipasa C.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012086378 | 2012-12-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR093923A1 true AR093923A1 (es) | 2015-06-24 |
Family
ID=50933768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130104633A AR093923A1 (es) | 2012-12-11 | 2013-12-11 | Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codifican |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US20150368627A1 (es) |
| EP (1) | EP2931744B1 (es) |
| CN (2) | CN112852777A (es) |
| AR (1) | AR093923A1 (es) |
| BR (1) | BR112015013399B1 (es) |
| RU (1) | RU2015126866A (es) |
| WO (1) | WO2014090161A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR093923A1 (es) * | 2012-12-11 | 2015-06-24 | Novozymes As | Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codifican |
| CN106459935A (zh) * | 2014-03-27 | 2017-02-22 | 诺维信公司 | 具有磷脂酶c活性的多肽和编码它们的多核苷酸 |
| AR104205A1 (es) | 2015-04-09 | 2017-07-05 | Dsm Ip Assets Bv | Fosfolipasa c |
| WO2017112540A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Processes for producing fermentation products |
| EP3401383A1 (en) | 2017-05-08 | 2018-11-14 | Bunge Oils, Inc. | Process for enzymatic degumming |
| CN113286889A (zh) * | 2018-10-08 | 2021-08-20 | 诺维信公司 | 用于生产乙醇的表达酶的酵母 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8525012D0 (en) | 1985-10-10 | 1985-11-13 | Cpc International Inc | Carbohydrate refining process |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| BR9507817A (pt) | 1994-06-03 | 1997-09-16 | Novo Nordisk Biotech Inc | Construção de dna enzima vetor recombinante célula hospedeira recombinante lacase de ascomicete ou deuteromicete processos para obter uma enzima de lacase para melhorar o rendimento de enzima recombinante para polimerizar um substrato de lignina ou lignossulfato em soluç o para despolimerizar in situ a pasta kraft para oxidar corantes ou precursores de corantes para pintar cabelo e para polimerizar ou oxidar um composto fenólico ou anilina composição de corante e recipiente contendo a mesma |
| CN1151762A (zh) | 1994-06-30 | 1997-06-11 | 诺沃诺尔迪斯克生物技术有限公司 | 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子 |
| DE19620649A1 (de) | 1996-05-22 | 1997-11-27 | Roehm Gmbh | Rekombinant hergestellte Lysophospholipase aus Aspergillus |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| ATE332968T1 (de) | 1998-10-26 | 2006-08-15 | Novozymes As | Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen |
| CN100482801C (zh) | 1999-03-22 | 2009-04-29 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
| CN101432409A (zh) | 2006-03-01 | 2009-05-13 | 嘉吉有限公司 | 甘油三酯油的脱胶方法 |
| CN102174488A (zh) * | 2006-03-10 | 2011-09-07 | 三菱化学食品株式会社 | 磷脂酶c及含其的食品用酶剂、以及食品原料的处理法 |
| EP2368978B1 (en) * | 2006-03-10 | 2014-04-30 | Mitsubishi-Kagaku Foods Corporation | Novel phospholipase C enzyme(s) |
| MX2009003034A (es) * | 2006-09-21 | 2009-11-18 | Verenium Corp | Fosfolipasas, acidos nucleicos que las codifican, y metodos de hacerlas y usarlas. |
| US8956853B2 (en) * | 2007-01-30 | 2015-02-17 | Bunge Oils, Inc. | Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases |
| CN105038978B (zh) * | 2007-01-30 | 2017-09-29 | 邦奇油类公司 | 使用pla和plc磷脂酶混合物进行的酶法脱胶 |
| RU2013126882A (ru) * | 2010-11-12 | 2014-12-20 | Новозимс А/С | Полипептиды, обладающие активностью фосфолипазы с, и полинуклеотиды, кодирующие их |
| AR093923A1 (es) | 2012-12-11 | 2015-06-24 | Novozymes As | Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codifican |
-
2013
- 2013-12-11 AR ARP130104633A patent/AR093923A1/es unknown
- 2013-12-11 US US14/649,084 patent/US20150368627A1/en not_active Abandoned
- 2013-12-11 CN CN202110040263.5A patent/CN112852777A/zh active Pending
- 2013-12-11 BR BR112015013399-1A patent/BR112015013399B1/pt active IP Right Grant
- 2013-12-11 WO PCT/CN2013/089106 patent/WO2014090161A1/en active Application Filing
- 2013-12-11 EP EP13862718.7A patent/EP2931744B1/en active Active
- 2013-12-11 CN CN201380064230.8A patent/CN104884464B/zh active Active
- 2013-12-11 RU RU2015126866A patent/RU2015126866A/ru not_active Application Discontinuation
-
2018
- 2018-10-04 US US16/152,043 patent/US10745676B2/en active Active
-
2020
- 2020-07-07 US US16/922,498 patent/US11180742B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112852777A (zh) | 2021-05-28 |
| US20190024063A1 (en) | 2019-01-24 |
| RU2015126866A (ru) | 2017-01-19 |
| US20150368627A1 (en) | 2015-12-24 |
| EP2931744A1 (en) | 2015-10-21 |
| WO2014090161A1 (en) | 2014-06-19 |
| EP2931744B1 (en) | 2021-08-18 |
| CN104884464B (zh) | 2021-02-02 |
| EP2931744A4 (en) | 2016-08-17 |
| CN104884464A (zh) | 2015-09-02 |
| BR112015013399A2 (pt) | 2017-11-14 |
| US20200332270A1 (en) | 2020-10-22 |
| US10745676B2 (en) | 2020-08-18 |
| BR112015013399B1 (pt) | 2023-02-14 |
| US11180742B2 (en) | 2021-11-23 |
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