AR093923A1 - Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codifican - Google Patents

Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codifican

Info

Publication number
AR093923A1
AR093923A1 ARP130104633A ARP130104633A AR093923A1 AR 093923 A1 AR093923 A1 AR 093923A1 AR P130104633 A ARP130104633 A AR P130104633A AR P130104633 A ARP130104633 A AR P130104633A AR 093923 A1 AR093923 A1 AR 093923A1
Authority
AR
Argentina
Prior art keywords
polypeptide
phospholipase
activity
polypeptides
seq
Prior art date
Application number
ARP130104633A
Other languages
English (en)
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of AR093923A1 publication Critical patent/AR093923A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
    • A23D9/013—Other fatty acid esters, e.g. phosphatides
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C11B3/04—Refining fats or fatty oils by chemical reaction with acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/04—Phosphoric diester hydrolases (3.1.4)
    • C12Y301/04003—Phospholipase C (3.1.4.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Edible Oils And Fats (AREA)

Abstract

La presente se relaciona con polipéptidos aislados que tienen actividad de fosfolipasa C, y polinucleótidos que codifican a los polipéptidos. La solicitud también se relaciona con constructos de ácidos nucleicos, vectores y células huésped que comprenden los polinucleótidos como así también métodos para producir y utilizar los polipéptidos. Reivindicación 1: Un polipéptido aislado que tiene actividad de fosfolipasa C, seleccionado del grupo que consiste en: a) un polipéptido que tiene al menos 90%, al menos 91%, al menos 92%, al menos 93%, al menos 94%, al menos 95%, al menos 96%, al menos 97%, al menos 98%, al menos 99% o 100% de identidad de secuencia con el polipéptido maduro de la SEC ID Nº 2; b) un polipéptido codificado por un polinucleótido que se hibrida en condiciones muy restrictivas con i) el polipéptido maduro que codifica la secuencia SEC ID Nº 1, o ii) el complemento de longitud completa de i); c) un polipéptido codificado por un polinucleótido que tiene al menos 90%, al menos 91%, al menos 92%, al menos 93%, al menos 94%, al menos 95%, al menos 96%, al menos 97%, al menos 98%, al menos 99% o 100% de identidad de secuencia con el polipéptido maduro de la SEC ID Nº 1; d) una variante del polipéptido maduro de la SEC ID Nº 2 que comprende una sustitución, supresión y/o inserción en una o más posiciones; y e) un fragmento del polipéptido de a), b), c) o d) que tiene actividad de fosfolipasa C.
ARP130104633A 2012-12-11 2013-12-11 Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codifican AR093923A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2012086378 2012-12-11

Publications (1)

Publication Number Publication Date
AR093923A1 true AR093923A1 (es) 2015-06-24

Family

ID=50933768

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130104633A AR093923A1 (es) 2012-12-11 2013-12-11 Polipeptidos con actividad fosfolipasa c y polinucleotidos que los codifican

Country Status (7)

Country Link
US (3) US20150368627A1 (es)
EP (1) EP2931744B1 (es)
CN (2) CN112852777A (es)
AR (1) AR093923A1 (es)
BR (1) BR112015013399B1 (es)
RU (1) RU2015126866A (es)
WO (1) WO2014090161A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112015013399B1 (pt) * 2012-12-11 2023-02-14 Novozymes A/S Uso de polipeptídeo tendo atividade de fosfolipase c e método para redução do conteúdo de componentes contendo fósforo em um óleo comestível
WO2015144092A1 (en) * 2014-03-27 2015-10-01 Novozymes A/S Polypeptides having phospholipase c activity and polynucleotides encoding same
AR104205A1 (es) 2015-04-09 2017-07-05 Dsm Ip Assets Bv Fosfolipasa c
US20190010470A1 (en) 2015-12-22 2019-01-10 Novozymes A/S Processes for producing fermentation products
EP3401383A1 (en) 2017-05-08 2018-11-14 Bunge Oils, Inc. Process for enzymatic degumming
CA3114783A1 (en) * 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production

Family Cites Families (21)

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Publication number Priority date Publication date Assignee Title
GB8525012D0 (en) 1985-10-10 1985-11-13 Cpc International Inc Carbohydrate refining process
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
AU694954B2 (en) 1994-06-03 1998-08-06 Novo Nordisk A/S Purified myceliophthora laccases and nucleic acids encoding same
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
DE19620649A1 (de) 1996-05-22 1997-11-27 Roehm Gmbh Rekombinant hergestellte Lysophospholipase aus Aspergillus
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
US20090306419A1 (en) 2006-03-01 2009-12-10 Cargill, Incorporated Method for Degumming Triglyceride Oils
EP1995313B1 (en) * 2006-03-10 2013-05-08 Mitsubishi-Kagaku Foods Corporation Novel phospholipase c
CN102174488A (zh) * 2006-03-10 2011-09-07 三菱化学食品株式会社 磷脂酶c及含其的食品用酶剂、以及食品原料的处理法
EA015591B1 (ru) * 2006-09-21 2011-10-31 Верениум Корпорейшн Фосфолипазы, кодирующие их нуклеиновые кислоты и способы их получения и применения
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MX356647B (es) * 2010-11-12 2018-06-07 Novozymes As Polipeptidos que tienen actividad de fosfolipasa c y polinucleotidos que codifican los mismos.
BR112015013399B1 (pt) 2012-12-11 2023-02-14 Novozymes A/S Uso de polipeptídeo tendo atividade de fosfolipase c e método para redução do conteúdo de componentes contendo fósforo em um óleo comestível

Also Published As

Publication number Publication date
US11180742B2 (en) 2021-11-23
US20200332270A1 (en) 2020-10-22
EP2931744A1 (en) 2015-10-21
CN104884464B (zh) 2021-02-02
CN112852777A (zh) 2021-05-28
US10745676B2 (en) 2020-08-18
EP2931744A4 (en) 2016-08-17
WO2014090161A1 (en) 2014-06-19
BR112015013399A2 (pt) 2017-11-14
RU2015126866A (ru) 2017-01-19
EP2931744B1 (en) 2021-08-18
US20190024063A1 (en) 2019-01-24
US20150368627A1 (en) 2015-12-24
CN104884464A (zh) 2015-09-02
BR112015013399B1 (pt) 2023-02-14

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