AR105766A1 - Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción - Google Patents

Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción

Info

Publication number
AR105766A1
AR105766A1 ARP160102214A ARP160102214A AR105766A1 AR 105766 A1 AR105766 A1 AR 105766A1 AR P160102214 A ARP160102214 A AR P160102214A AR P160102214 A ARP160102214 A AR P160102214A AR 105766 A1 AR105766 A1 AR 105766A1
Authority
AR
Argentina
Prior art keywords
pululanase
liquefaction
polypeptides
activity
variant
Prior art date
Application number
ARP160102214A
Other languages
English (en)
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of AR105766A1 publication Critical patent/AR105766A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Emergency Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente se refiere a una pululanasa variante, en donde la pululanasa comprende por lo menos la siguiente combinación de sustituciones: N368G+N393A+Q431E+L432F+A492A,S+N610R+G624S+T631S+S632C, y opcionalmente además comprende N222P+Q252A+Q256R; en donde la variante tiene actividad de pululanasa, y en donde las variantes tienen por lo menos 85%, por lo menos 90%, por lo menos 95%, por lo menos 96%, por lo menos 97%, por lo menos 98%, o por lo menos 99%, pero menos de 100%, de identidad de secuencia con el polipéptido de SEQ ID Nº 3. En otro aspecto, la presente se refiere a un proceso para la licuefacción de material que contiene almidón a una temperatura por encima de la temperatura de gelatinización inicial, empleando una a-amilasa y una pululanasa termoestable
ARP160102214A 2015-07-21 2016-07-21 Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción AR105766A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562194982P 2015-07-21 2015-07-21

Publications (1)

Publication Number Publication Date
AR105766A1 true AR105766A1 (es) 2017-11-08

Family

ID=56550366

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160102214A AR105766A1 (es) 2015-07-21 2016-07-21 Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción

Country Status (8)

Country Link
US (1) US11427811B2 (es)
EP (1) EP3325617B1 (es)
CN (1) CN107922933A (es)
AR (1) AR105766A1 (es)
CA (1) CA2989314A1 (es)
DK (1) DK3325617T3 (es)
ES (1) ES2903092T3 (es)
WO (1) WO2017014974A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108384770B (zh) * 2018-03-01 2019-11-22 江南大学 一种降低环糊精对普鲁兰酶抑制作用的方法
CN108913677B (zh) * 2018-07-23 2021-06-29 福州大学 一种定点突变改造的碱性普鲁兰酶及其应用
CN113490740A (zh) * 2019-03-18 2021-10-08 诺维信公司 适合用于液化中的具有普鲁兰酶活性的多肽
CN114007566A (zh) 2019-04-09 2022-02-01 弗拉基米尔·斯维里多维奇·梅德韦夫 可重复使用的尿布
CN111826368B (zh) * 2020-07-23 2021-11-23 中国农业科学院农产品加工研究所 I型普鲁兰酶的突变体酶及其制备方法与应用
CN114790451B (zh) * 2020-12-01 2023-12-19 天津科技大学 一种普鲁兰酶及其应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2137222T3 (es) 1992-12-28 1999-12-16 Genencor Int Polulanasa, microorganismos que la producen, procedimientos para la preparacion de la misma y utilizaciones.
EP1250423B1 (en) * 2000-01-12 2008-09-03 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
CN101918525A (zh) 2007-12-12 2010-12-15 诺维信公司 糖化方法
WO2011076123A1 (en) 2009-12-22 2011-06-30 Novozymes A/S Compositions comprising boosting polypeptide and starch degrading enzyme and uses thereof
US9951323B2 (en) * 2013-07-17 2018-04-24 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
HUE056192T2 (hu) * 2014-01-22 2022-01-28 Novozymes As Pullulanázvariánsok és azokat kódoló polinukleotidok

Also Published As

Publication number Publication date
EP3325617A1 (en) 2018-05-30
CA2989314A1 (en) 2017-01-26
US20180208917A1 (en) 2018-07-26
WO2017014974A1 (en) 2017-01-26
ES2903092T3 (es) 2022-03-31
CN107922933A (zh) 2018-04-17
DK3325617T3 (da) 2022-01-10
US11427811B2 (en) 2022-08-30
EP3325617B1 (en) 2021-10-27

Similar Documents

Publication Publication Date Title
AR105766A1 (es) Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción
MX2022003497A (es) Procesos para producir productos de fermentacion.
PH12016501671A1 (en) Novel polysaccharide and uses thereof
PE20170950A1 (es) Polipeptidos del factor de crecimiento de fibroblastos 2 (fgf-21) modificados y usos de los mismos
CA166474S (en) Bottle
MY191210A (en) Novel promoter and uses thereof
AU366476S (en) Bottle
EA201791426A1 (ru) Полипептид и иммуномодуляция
PH12015501983B1 (en) Peptides and compositions for treatment of joint damage
AR096869A1 (es) Neurotoxinas catiónicas
PE20170908A1 (es) VARIANTES DE INTERFERON a2b
ES2721309T3 (es) Composición para preparar tagatosa y método para preparar tagatosa a partir de fructosa
BR112016027871A2 (pt) composição para melhorar a memória, a função de aprendizado e/ou função cognitiva
AR100088A1 (es) Fluido de tratamiento
AR100606A1 (es) Variantes de lipasas y polinucleótidos que las codifican
AR100942A1 (es) Composiciones y método para el control glucémico que comprenden albiglutida
PE20170955A1 (es) Nuevas proteinas inhibidoras de insectos
AR111373A1 (es) Enzimas de lactasa con propiedades mejoradas
ZA201704903B (en) Modified dnase and uses thereof
CO2018000194A2 (es) Alelos de dominio mads-box para controlar el fenotipo de membrana en la palma
AR102950A1 (es) Variantes de lipasa y polinucleótidos que las codifican
AR102919A1 (es) Variantes de lipasa y polinucleótidos que las codifican
BR112017003228A2 (pt) genes chromobacterium subtsugae
MX2018014621A (es) Composicion biopesticida para uso en la prevencion o para minimizar la enfermedad de la planta.
GB201916135D0 (en) Extracellular matrix gels, and organoid cultures comprising the same