AR105766A1 - Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción - Google Patents

Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción

Info

Publication number
AR105766A1
AR105766A1 ARP160102214A ARP160102214A AR105766A1 AR 105766 A1 AR105766 A1 AR 105766A1 AR P160102214 A ARP160102214 A AR P160102214A AR P160102214 A ARP160102214 A AR P160102214A AR 105766 A1 AR105766 A1 AR 105766A1
Authority
AR
Argentina
Prior art keywords
pululanase
liquefaction
polypeptides
activity
variant
Prior art date
Application number
ARP160102214A
Other languages
English (en)
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of AR105766A1 publication Critical patent/AR105766A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

La presente se refiere a una pululanasa variante, en donde la pululanasa comprende por lo menos la siguiente combinación de sustituciones: N368G+N393A+Q431E+L432F+A492A,S+N610R+G624S+T631S+S632C, y opcionalmente además comprende N222P+Q252A+Q256R; en donde la variante tiene actividad de pululanasa, y en donde las variantes tienen por lo menos 85%, por lo menos 90%, por lo menos 95%, por lo menos 96%, por lo menos 97%, por lo menos 98%, o por lo menos 99%, pero menos de 100%, de identidad de secuencia con el polipéptido de SEQ ID Nº 3. En otro aspecto, la presente se refiere a un proceso para la licuefacción de material que contiene almidón a una temperatura por encima de la temperatura de gelatinización inicial, empleando una a-amilasa y una pululanasa termoestable
ARP160102214A 2015-07-21 2016-07-21 Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción AR105766A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562194982P 2015-07-21 2015-07-21

Publications (1)

Publication Number Publication Date
AR105766A1 true AR105766A1 (es) 2017-11-08

Family

ID=56550366

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160102214A AR105766A1 (es) 2015-07-21 2016-07-21 Polipéptidos que tienen actividad de pululanasa adecuados para usar en licuefacción

Country Status (8)

Country Link
US (1) US11427811B2 (es)
EP (1) EP3325617B1 (es)
CN (1) CN107922933A (es)
AR (1) AR105766A1 (es)
CA (1) CA2989314A1 (es)
DK (1) DK3325617T3 (es)
ES (1) ES2903092T3 (es)
WO (1) WO2017014974A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108384770B (zh) 2018-03-01 2019-11-22 江南大学 一种降低环糊精对普鲁兰酶抑制作用的方法
CN108913677B (zh) * 2018-07-23 2021-06-29 福州大学 一种定点突变改造的碱性普鲁兰酶及其应用
CA3128139A1 (en) 2019-03-18 2020-09-24 Novozymes A/S Polypeptides having pullulanase activity suitable for use in liquefaction
WO2020209757A2 (ru) 2019-04-09 2020-10-15 Владимир Свиридович МЕДВЕДЕВ Многоразовый подгузник
CN111826368B (zh) * 2020-07-23 2021-11-23 中国农业科学院农产品加工研究所 I型普鲁兰酶的突变体酶及其制备方法与应用
CN115197924B (zh) * 2020-12-01 2023-11-28 天津科技大学 一种普鲁兰酶
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0605040T3 (da) 1992-12-28 2000-03-13 Genencor Int Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf
AU2663801A (en) * 2000-01-12 2001-07-24 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
BRPI0722357A2 (pt) 2007-12-12 2014-03-18 Novozymes As Processo para produzir um mosto de cervejeiro, mosto, cerveja, e, composição
US20120252086A1 (en) 2009-12-22 2012-10-04 Novozymes A/S Compositions Comprising Boosting Polypeptide And Starch Degrading Enzyme And Uses Thereof
US9951323B2 (en) 2013-07-17 2018-04-24 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
HUE056192T2 (hu) 2014-01-22 2022-01-28 Novozymes As Pullulanázvariánsok és azokat kódoló polinukleotidok

Also Published As

Publication number Publication date
US11427811B2 (en) 2022-08-30
EP3325617B1 (en) 2021-10-27
CN107922933A (zh) 2018-04-17
EP3325617A1 (en) 2018-05-30
ES2903092T3 (es) 2022-03-31
US20180208917A1 (en) 2018-07-26
CA2989314A1 (en) 2017-01-26
DK3325617T3 (da) 2022-01-10
WO2017014974A1 (en) 2017-01-26

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