BR112014027283A2 - variantes de enzima aprimoradas - Google Patents

variantes de enzima aprimoradas

Info

Publication number
BR112014027283A2
BR112014027283A2 BR112014027283A BR112014027283A BR112014027283A2 BR 112014027283 A2 BR112014027283 A2 BR 112014027283A2 BR 112014027283 A BR112014027283 A BR 112014027283A BR 112014027283 A BR112014027283 A BR 112014027283A BR 112014027283 A2 BR112014027283 A2 BR 112014027283A2
Authority
BR
Brazil
Prior art keywords
variant
sequence
enzyme variants
enhanced enzyme
chymosin
Prior art date
Application number
BR112014027283A
Other languages
English (en)
Inventor
Marinus Muijlwijk Cornelis
Jacobus Theodorus Dekker Petrus
Marcel De Jong René
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of BR112014027283A2 publication Critical patent/BR112014027283A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/0684—Soft uncured Italian cheeses, e.g. Mozarella, Ricotta, Pasta filata cheese; Other similar stretched cheeses
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/072—Cheddar type or similar hard cheeses without eyes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C2220/00—Biochemical treatment
    • A23C2220/20—Treatment with microorganisms
    • A23C2220/202—Genetic engineering of microorganisms used in dairy technology
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C2250/00—Particular aspects related to cheese
    • A23C2250/15—Shredded non-dried cheese

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Dairy Products (AREA)

Abstract

resumo variantes de enzima aprimoradas trata-se de um polipeptídeo variante que tem atividade de quimosina, em que a variante tem uma sequência de aminoácidos que, quando alinhada com a quimosina que compreende a sequência apresentada em seq id no: 2, compreende pelo menos uma substituição de um resíduo de aminoácido correspondente a qualquer um dentre os aminoácidos 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 ou 295, em que as ditas posições são definidas com referência à seq id no: 2 e em que a variante tem uma ou mais propriedades alteradas em comparação a um polipeptídeo de referência que tem atividade de quimosina. tal polipeptídeo variante pode ser usado na preparação de um queijo.
BR112014027283A 2012-05-03 2013-05-03 variantes de enzima aprimoradas BR112014027283A2 (pt)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201261642095P 2012-05-03 2012-05-03
EP12166673 2012-05-03
US201261745063P 2012-12-21 2012-12-21
EP12199178 2012-12-21
EP12199277 2012-12-21
PCT/EP2013/059315 WO2013164479A2 (en) 2012-05-03 2013-05-03 Improved enzyme variants

Publications (1)

Publication Number Publication Date
BR112014027283A2 true BR112014027283A2 (pt) 2017-08-08

Family

ID=49514227

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112014027371A BR112014027371A2 (pt) 2012-05-03 2013-05-03 variantes de enzima aprimoradas
BR112014027283A BR112014027283A2 (pt) 2012-05-03 2013-05-03 variantes de enzima aprimoradas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR112014027371A BR112014027371A2 (pt) 2012-05-03 2013-05-03 variantes de enzima aprimoradas

Country Status (12)

Country Link
US (3) US10167463B2 (pt)
EP (3) EP2844751B1 (pt)
AU (2) AU2013255749A1 (pt)
BR (2) BR112014027371A2 (pt)
CA (2) CA2872309C (pt)
DK (2) DK2844750T3 (pt)
EA (2) EA038324B1 (pt)
HK (2) HK1207880A1 (pt)
LT (2) LT2844750T (pt)
MX (2) MX355170B (pt)
NZ (1) NZ701125A (pt)
WO (2) WO2013164481A1 (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2855674T5 (pl) 2012-05-25 2023-03-27 Chr. Hansen A/S Warianty chymozyny o ulepszonych właściwościach ścinania mleka
CN111944791A (zh) * 2014-02-26 2020-11-17 科·汉森有限公司 具有提高的凝乳特性的凝乳酶变体
KR20170115614A (ko) 2015-02-10 2017-10-17 시에이치알. 한센 에이/에스 개선된 응유 특성을 갖는 키모신 배합물
EP3310914B1 (en) 2015-06-22 2022-04-06 Chr. Hansen A/S Variants of chymosin with improved properties
EP3344049A1 (en) * 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
EP3143879A1 (en) 2015-09-18 2017-03-22 DSM IP Assets B.V. Yogurt with reduced ripening
GB201517601D0 (en) * 2015-10-06 2015-11-18 Dale Farm Ltd Improvements relating to cheese making
EP3457860A1 (en) 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
CN109688825A (zh) * 2016-05-19 2019-04-26 科·汉森有限公司 具有改善的凝乳特性的凝乳酶的变体
CN106754840B (zh) * 2016-12-22 2019-06-21 江南大学 一种酶活及热稳定性改善的凝乳酶突变体
US11896022B2 (en) * 2018-01-11 2024-02-13 Chr. Hansen A/S Interactions between cultures, coagulants and technology to increase cheese yields
CN109182311B (zh) * 2018-10-11 2021-07-27 山东隆科特酶制剂有限公司 一种用于沂蒙黑山羊乳奶酪的凝乳酶
WO2023001832A1 (en) 2021-07-20 2023-01-26 Chr. Hansen A/S Composition for clotting milk, methods and uses thereof
WO2023194285A2 (en) 2022-04-06 2023-10-12 Chr. Hansen A/S Aspartic protease, methods and uses thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2354775T5 (es) * 2000-11-06 2021-12-23 Chr Hansen As Método de producción de quimosina no bovina y uso de la misma
WO2008008766A2 (en) * 2006-07-10 2008-01-17 Ohio University Co-expression of proline hydroxylases to facilitate hyp-glycosylation of proteins expressed and secreted in plant cells
EP3763220A1 (en) * 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk

Also Published As

Publication number Publication date
CA2872309C (en) 2021-03-02
WO2013164479A2 (en) 2013-11-07
US10167463B2 (en) 2019-01-01
US20180030427A1 (en) 2018-02-01
EP3450552A2 (en) 2019-03-06
HK1207881A1 (en) 2016-02-12
LT2844750T (lt) 2018-09-10
EA201401211A1 (ru) 2015-05-29
EP2844751B1 (en) 2018-10-17
DK2844751T3 (en) 2019-02-04
WO2013164481A1 (en) 2013-11-07
MX2014013329A (es) 2015-02-10
EP2844750B1 (en) 2018-06-20
CA2872132C (en) 2020-06-16
AU2013255749A1 (en) 2014-11-06
CA2872309A1 (en) 2013-11-07
BR112014027371A2 (pt) 2017-08-08
EA201401209A1 (ru) 2015-05-29
CA2872132A1 (en) 2013-11-07
NZ701125A (en) 2016-11-25
HK1207880A1 (en) 2016-02-12
DK2844750T3 (en) 2018-10-01
EP3450552A3 (en) 2019-05-08
US10253305B2 (en) 2019-04-09
EP2844751A2 (en) 2015-03-11
EA035275B1 (ru) 2020-05-22
AU2013255747A1 (en) 2014-11-06
MX2014013333A (es) 2015-02-10
EP2844750A1 (en) 2015-03-11
MX357563B (es) 2018-07-13
WO2013164479A3 (en) 2013-12-19
MX355170B (es) 2018-04-05
EA038324B1 (ru) 2021-08-10
LT2844751T (lt) 2019-02-11
US9822352B2 (en) 2017-11-21
US20150118356A1 (en) 2015-04-30
US20150140169A1 (en) 2015-05-21

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]