BR112015020692A2 - variantes de alfa-amilase - Google Patents

variantes de alfa-amilase

Info

Publication number
BR112015020692A2
BR112015020692A2 BR112015020692A BR112015020692A BR112015020692A2 BR 112015020692 A2 BR112015020692 A2 BR 112015020692A2 BR 112015020692 A BR112015020692 A BR 112015020692A BR 112015020692 A BR112015020692 A BR 112015020692A BR 112015020692 A2 BR112015020692 A2 BR 112015020692A2
Authority
BR
Brazil
Prior art keywords
alpha
variant
amylase
amylase variants
polypeptide
Prior art date
Application number
BR112015020692A
Other languages
English (en)
Inventor
Tjeerd Van Rij Evert
Elisabet Abbas Hanna
Marcel De Jong René
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/842,344 external-priority patent/US9301533B2/en
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of BR112015020692A2 publication Critical patent/BR112015020692A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D10/00—Batters, dough or mixtures before baking
    • A21D10/002—Dough mixes; Baking or bread improvers; Premixes
    • A21D10/005—Solid, dry or compact materials; Granules; Powders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Peptides Or Proteins (AREA)

Abstract

resumo “variantes de alfa-amilase” um polipeptídeo variante que possui atividade de alfa-amilase, em que a variante tem uma sequência de aminoácidos que, quando alinhada com a alfa-amilase que compreende a sequência apresentada no id. de seq. nº: 2, compreende pelo menos uma substituição de um resíduo de aminoácido que corresponde a qualquer um dos aminoácidos 4, 6, 13, 14, 15, 16, 20, 45, 47, 51, 54, 61, 68, 69, 70, 71, 72, 73, 74, 75, 77, 78, 80, 82, 87, 88, 94, 95, 100, 103, 104, 117, 124, 125, 126, 128, 130, 133, 134, 136, 143, 144, 146, 168, 174, 177, 178, 183, 186, 188, 189, 190, 194, 195, 199, 200, 201, 204, 207, 210, 214, 217, 219, 222, 225, 227, 233, 234, 235, 236, 240, 251, 252, 254, 258, 259, 260, 261, 262, 263, 264, 266, 267, 269, 271, 273, 281, 282, 283, 284, 286, 288, 299, 322, 323, 325, 327, 328, 331, 334, 350, 356, 358, 367, 370, 371, 374, 377, 378, 388, 391, 414, 421, 422, 445, 450, 467, 488, 505, 536, 548, 554, 583, 588, 603, 637, 648, 651, 652, 660, 676, 677, as referidas posições sendo definidas com referência ao id. de seq. nº: 2 e em que a variante possui uma ou mais propriedades alteradas quando comparada com um polipeptídeo de referência que possui atividade de alfa-amilase. tal polipeptídeo variante pode ser usado na preparação de um produto assado.
BR112015020692A 2013-03-01 2014-02-27 variantes de alfa-amilase BR112015020692A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361771676P 2013-03-01 2013-03-01
EP13157398 2013-03-01
US13/842,344 US9301533B2 (en) 2013-03-01 2013-03-15 Alpha-amylase variants
EP13183144 2013-09-05
PCT/EP2014/053853 WO2014131842A1 (en) 2013-03-01 2014-02-27 Alpha-amylase variants

Publications (1)

Publication Number Publication Date
BR112015020692A2 true BR112015020692A2 (pt) 2017-10-10

Family

ID=51427546

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015020692A BR112015020692A2 (pt) 2013-03-01 2014-02-27 variantes de alfa-amilase

Country Status (8)

Country Link
US (1) US10327449B2 (pt)
EP (1) EP2961839B1 (pt)
CN (1) CN105026557A (pt)
AU (1) AU2014222636A1 (pt)
BR (1) BR112015020692A2 (pt)
CA (1) CA2902996A1 (pt)
EA (1) EA201591609A1 (pt)
WO (1) WO2014131842A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016030448A1 (en) * 2014-08-28 2016-03-03 Dsm Ip Assets B.V. Alicyclobacillus pohliae alpha-amylase variants
CN106434601B (zh) * 2016-10-18 2019-06-28 河北华石生物科技有限公司 一种突变的α-淀粉酶及其制备方法和应用
WO2019193102A1 (en) 2018-04-05 2019-10-10 Dsm Ip Assets B.V. Variant maltogenic alpha-amylase
CN109370973B (zh) * 2018-11-22 2020-12-29 湖南汇升生物科技有限公司 一种麦芽糖淀粉酶生产菌株
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114891757B (zh) * 2022-06-13 2024-01-23 安徽大学 一种漆酶定向进化方法及突变漆酶基因、表达载体、重组菌株及其脱毒黄曲霉毒素应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
USRE38507E1 (en) 1989-09-27 2004-04-27 Novozymes A/S Antistaling process and agent
AU761751B2 (en) 1998-02-27 2003-06-12 Novozymes A/S Amylolytic enzyme variants
ATE490312T1 (de) * 1998-02-27 2010-12-15 Novozymes As Maltogene alpha-amylase varianten
GB0305685D0 (en) * 2003-03-12 2003-04-16 Danisco Enzyme
CA2575878A1 (en) * 2004-08-02 2006-02-09 Novozymes A/S Creation of diversity in polypeptides
AU2005269079A1 (en) * 2004-08-02 2006-02-09 Novozymes A/S Maltogenic alpha-amylase variants
EP2164958B1 (en) * 2007-06-07 2014-08-13 Novozymes A/S Method of preparing a dough-based product
DK2499227T3 (en) * 2009-11-13 2015-07-13 Novozymes As A method for brewing

Also Published As

Publication number Publication date
AU2014222636A1 (en) 2015-10-22
EP2961839A1 (en) 2016-01-06
EP2961839B1 (en) 2018-11-07
WO2014131842A1 (en) 2014-09-04
CA2902996A1 (en) 2014-09-04
CN105026557A (zh) 2015-11-04
US10327449B2 (en) 2019-06-25
EA201591609A1 (ru) 2016-03-31
US20180007917A1 (en) 2018-01-11

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Legal Events

Date Code Title Description
B08F Application fees: dismissal - article 86 of industrial property law

Free format text: REFERENTE A 4A ANUIDADE.

B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)