BR112014031942A2 - enzimas e métodos para síntese de estireno - Google Patents

enzimas e métodos para síntese de estireno

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Publication number
BR112014031942A2
BR112014031942A2 BR112014031942A BR112014031942A BR112014031942A2 BR 112014031942 A2 BR112014031942 A2 BR 112014031942A2 BR 112014031942 A BR112014031942 A BR 112014031942A BR 112014031942 A BR112014031942 A BR 112014031942A BR 112014031942 A2 BR112014031942 A2 BR 112014031942A2
Authority
BR
Brazil
Prior art keywords
styrene
enzymes
tca
phenylalanine
methods
Prior art date
Application number
BR112014031942A
Other languages
English (en)
Inventor
Chen Hui
Han Jixiang
Wadud Bhuiya Mohammad
Cai Xianpeng
Yu Xiaodan
Original Assignee
Phytogene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phytogene Inc filed Critical Phytogene Inc
Publication of BR112014031942A2 publication Critical patent/BR112014031942A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C12P5/005—Preparation of hydrocarbons or halogenated hydrocarbons cyclic aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01024—Phenylalanine ammonia-lyase (4.3.1.24)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01005—Phenylalanine ammonia-lyase (4.3.1.5)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

resumo patente de invenção: "enzimas e métodos para síntese de estireno". a presente tecnologia desta invenção refere-se em geral a biossíntese de estireno. certas modalidades da presente tecnologia baseiam-se, em parte, ao reconhecimento de que fenilalanina pode ser convertida em estireno por uma via de duas etapas de desaminação e descarboxilação, com ácido trans-cinâmico (tca) como intermediário. dois tipos de enzimas estão diretamente envolvidos nesse processo, fenilalanina amônia liase (pal), que converte fenilalanina em tca, e ácido cinâmico descarboxilase, que coverte tca em estireno. células hospedeiras que expressam esses dois tipos de enzimas podem ser cultivadas em biorreator para produzir estireno a partir de substratos renováveis, como a glicose.
BR112014031942A 2012-06-22 2013-06-21 enzimas e métodos para síntese de estireno BR112014031942A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261663500P 2012-06-22 2012-06-22
PCT/US2013/047098 WO2013192543A2 (en) 2012-06-22 2013-06-21 Enzymes and methods for styrene synthesis

Publications (1)

Publication Number Publication Date
BR112014031942A2 true BR112014031942A2 (pt) 2017-08-01

Family

ID=49769723

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014031942A BR112014031942A2 (pt) 2012-06-22 2013-06-21 enzimas e métodos para síntese de estireno

Country Status (8)

Country Link
US (2) US20150337336A1 (pt)
EP (1) EP2864491B1 (pt)
CN (1) CN104487581B (pt)
BR (1) BR112014031942A2 (pt)
DK (1) DK2864491T3 (pt)
ES (1) ES2709217T3 (pt)
LT (1) LT2864491T (pt)
WO (1) WO2013192543A2 (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2709217T3 (es) 2012-06-22 2019-04-15 Phytogene Inc Enzimas y métodos para la síntesis del estireno
WO2016183531A1 (en) 2015-05-13 2016-11-17 Synlogic, Inc. Bacteria engineered to reduce hyperphenylalaninemia
JP6993970B2 (ja) * 2015-11-16 2022-02-15 シンロジック オペレーティング カンパニー インコーポレイテッド 高フェニルアラニン血症を低減させるように操作された細菌
IL271085B2 (en) 2017-06-21 2024-07-01 Synlogic Operating Co Inc Bacteria to treat disorders
CN109136158A (zh) * 2017-06-27 2019-01-04 中国科学院青岛生物能源与过程研究所 一种以生物质水解液为原料合成苯乙烯的基因工程菌及其构建方法与应用
US11142778B2 (en) 2017-07-24 2021-10-12 Riken Decarboxylase and method for producing unsaturated hydrocarbon compound using same
CN115298308A (zh) 2020-03-20 2022-11-04 同生运营公司 经工程化以减轻高苯丙氨酸血症的微生物
WO2022145178A1 (ja) * 2020-12-28 2022-07-07 国立研究開発法人理化学研究所 フェニルアラニンアンモニアリアーゼを用いた鎖状の不飽和カルボン酸化合物の製造方法
WO2023044381A1 (en) * 2021-09-16 2023-03-23 Arcturus Therapeutics, Inc. Compositions and methods for treating phenylketonuria
CN115960935A (zh) * 2022-09-30 2023-04-14 大连理工大学 一种利用阿魏酸脱羧酶高效催化肉桂酸合成苯乙烯的方法
CN115851797A (zh) * 2022-11-01 2023-03-28 大连理工大学 一种高效供给阿魏酸脱羧酶的辅因子prFMN的工程菌及构建方法与应用
CN118086266B (zh) * 2023-11-22 2024-10-25 大连理工大学 一种阿魏酸脱羧酶高活性组合突变体及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6087557A (en) 1992-10-06 2000-07-11 The Salk Institute For Biological Studies Methods for the modulation of lignification levels
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
EP1589112A1 (en) * 2004-04-21 2005-10-26 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO Microbial production of aromatic acids
US20090311760A1 (en) 2006-05-17 2009-12-17 Schoemakerstraat 97 Host cells and uses thereof in the microbial production of hydroxylated aromatics
CN101397568A (zh) 2008-10-31 2009-04-01 云南大学 一种细菌阿魏酸脱羧酶基因及其晶体结构
WO2012122333A1 (en) * 2011-03-08 2012-09-13 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Microbial conversion of glucose to styrene and its derivatives
ES2709217T3 (es) * 2012-06-22 2019-04-15 Phytogene Inc Enzimas y métodos para la síntesis del estireno
WO2014202838A1 (en) * 2013-06-20 2014-12-24 Teknologian Tutkimuskeskus Vtt Methods for producing styrene and genetically modified micro-organisms related thereto
CN103865912B (zh) * 2014-02-24 2016-05-25 无锡新和源发酵技术研究院有限公司 一种阿魏酸脱羧酶晶体结构及其解析方法

Also Published As

Publication number Publication date
ES2709217T3 (es) 2019-04-15
US20180002726A1 (en) 2018-01-04
WO2013192543A3 (en) 2014-05-08
DK2864491T3 (en) 2018-12-17
EP2864491B1 (en) 2018-10-17
CN104487581A (zh) 2015-04-01
CN104487581B (zh) 2021-05-28
WO2013192543A2 (en) 2013-12-27
EP2864491A4 (en) 2016-05-11
LT2864491T (lt) 2018-12-27
US20150337336A1 (en) 2015-11-26
US10752921B2 (en) 2020-08-25
EP2864491A2 (en) 2015-04-29

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements