BR112015010134A2 - Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicas - Google Patents

Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicas

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Publication number
BR112015010134A2
BR112015010134A2 BR112015010134A BR112015010134A BR112015010134A2 BR 112015010134 A2 BR112015010134 A2 BR 112015010134A2 BR 112015010134 A BR112015010134 A BR 112015010134A BR 112015010134 A BR112015010134 A BR 112015010134A BR 112015010134 A2 BR112015010134 A2 BR 112015010134A2
Authority
BR
Brazil
Prior art keywords
acetate
conversion
ethanol
biomass
isopropanol
Prior art date
Application number
BR112015010134A
Other languages
English (en)
Inventor
Meindert Zelle Rintze
J Shaw Arthur Iv
Pieter Van Dijken Johannes
Original Assignee
Lallemand Hungary Liquidity Man Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lallemand Hungary Liquidity Man Llc filed Critical Lallemand Hungary Liquidity Man Llc
Publication of BR112015010134A2 publication Critical patent/BR112015010134A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01175—D-Xylose 1-dehydrogenase (1.1.1.175)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01307—D-Xylose reductase (1.1.1.307)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MICRO-ORGANISMOS RECOMBINANTES, VIA METABÓLICA ENGENHEIRADA E PROCESSOS PARA AUMENTAR A CAPTAÇÃO DE ACETATO PARTINDO DE UMA BIOMASSA E PARA CONVERSÃO DE BIOMASSA EM ETANOL, ACETONA OU ISOPROPANOL. A presente invenção refere-se a novas vias metabólicas para desintoxicar acetato derivado da biomassa através da conversão metabólica em etanol, acetona ou isopropanol. Mais especificamente, a invenção refere-se a um micro-organismo recombinante que compreende uma ou mais enzimas nativas e/ou heterólogas que funcionam em uma ou mais primeiras vias metabólicas engenheiradas para atingir: (1) conversão de acetato em etanol; (2) conversão de acetato em acetona; ou (3) conversão de acetato em isopropanol; e uma ou mais enzimas nativas e/ou heterólogas que funcionam em uma ou mais segundas vias metabólicas engenheiradas para produzir um doador de elétron utilizado na conversão de acetato em compostos menos inibidores; em que a uma ou mais enzimas nativas e/ou heterólogas são ativadas, reguladas para mais ou reguladas para menos.
BR112015010134A 2012-11-09 2013-11-08 Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicas BR112015010134A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261724831P 2012-11-09 2012-11-09
US201361793716P 2013-03-15 2013-03-15
PCT/US2013/069266 WO2014074895A2 (en) 2012-11-09 2013-11-08 Method for acetate consumption during ethanolic fermentation of cellulosic feedstocks

Publications (1)

Publication Number Publication Date
BR112015010134A2 true BR112015010134A2 (pt) 2017-08-22

Family

ID=49880925

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015010134A BR112015010134A2 (pt) 2012-11-09 2013-11-08 Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicas

Country Status (6)

Country Link
US (4) US20140256011A1 (pt)
EP (2) EP2917343A2 (pt)
CN (1) CN104903441A (pt)
BR (1) BR112015010134A2 (pt)
CA (1) CA2889890C (pt)
WO (1) WO2014074895A2 (pt)

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JP6879111B2 (ja) * 2017-08-02 2021-06-02 トヨタ自動車株式会社 組換え酵母及びこれを用いたエタノールの製造方法
CA3091962A1 (en) 2017-09-19 2019-03-28 Lallemand Hungary Liquidity Management Llc Acetate toxicity tolerance in recombinant microbial host cells
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Also Published As

Publication number Publication date
US20160265005A1 (en) 2016-09-15
CA2889890A1 (en) 2014-05-15
CA2889890C (en) 2023-03-28
US20140256011A1 (en) 2014-09-11
WO2014074895A2 (en) 2014-05-15
US20200095613A1 (en) 2020-03-26
EP3498829A1 (en) 2019-06-19
EP2917343A2 (en) 2015-09-16
US20210277427A1 (en) 2021-09-09
CN104903441A (zh) 2015-09-09
WO2014074895A3 (en) 2014-07-03

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B25G Requested change of headquarter approved

Owner name: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC (HU)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements