AR128162A1 - Glutamato deshidrogenasa modificada y uso de la misma - Google Patents
Glutamato deshidrogenasa modificada y uso de la mismaInfo
- Publication number
- AR128162A1 AR128162A1 ARP220103649A ARP220103649A AR128162A1 AR 128162 A1 AR128162 A1 AR 128162A1 AR P220103649 A ARP220103649 A AR P220103649A AR P220103649 A ARP220103649 A AR P220103649A AR 128162 A1 AR128162 A1 AR 128162A1
- Authority
- AR
- Argentina
- Prior art keywords
- present
- modified
- gludh
- glutamate dehydrogenase
- glufosinate
- Prior art date
Links
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 title abstract 7
- 102000016901 Glutamate dehydrogenase Human genes 0.000 title abstract 7
- 150000002306 glutamic acid derivatives Chemical class 0.000 title abstract 2
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 abstract 2
- YJTNHDYMQPHXFO-UHFFFAOYSA-N 4-(hydroxymethylphosphinyl)-2-oxobutyric acid Chemical compound CP(O)(=O)CCC(=O)C(O)=O YJTNHDYMQPHXFO-UHFFFAOYSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente invención se refiere a una glutamato deshidrogenasa (GluDH) modificada. En particular la GluDH modificada de la presente invención tiene una actividad aumentada para catalizar la reacción de ácido 4-(hidroximetilfosfinil)-2-oxobutanoico (PPO) y un donante de amino para generar L-glufosinato y/o una propiedad dinámica mejorada. La presente invención también se refiere al polinucleótido que codifica la GluDH modificada de la presente invención, el vector y célula huésped para expresar la GluDH modificada de la presente invención y el método para producir L-glufosinato con la GluDH modificada y la célula huésped de la presente invención.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111670071 | 2021-12-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR128162A1 true AR128162A1 (es) | 2024-03-27 |
Family
ID=86998100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220103649A AR128162A1 (es) | 2021-12-31 | 2022-12-29 | Glutamato deshidrogenasa modificada y uso de la misma |
Country Status (3)
| Country | Link |
|---|---|
| AR (1) | AR128162A1 (es) |
| TW (1) | TW202334407A (es) |
| WO (1) | WO2023125769A1 (es) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108588045B (zh) * | 2018-03-09 | 2019-12-10 | 浙江大学 | 谷氨酸脱氢酶突变体及其在制备l-草铵膦中的应用 |
| CN110343676B (zh) * | 2018-04-03 | 2020-06-23 | 上海弈柯莱生物医药科技有限公司 | 一种l-谷氨酸脱氢酶突变体及其应用 |
| CN110184246B (zh) * | 2019-05-15 | 2020-10-13 | 浙江大学 | 谷氨酸脱氢酶突变体及其应用 |
| CN111979208B (zh) * | 2019-05-23 | 2023-01-10 | 弈柯莱生物科技(上海)股份有限公司 | 一种l-谷氨酸脱氢酶突变体及其应用 |
| CN112553285B (zh) * | 2020-12-25 | 2022-10-28 | 浙江大学杭州国际科创中心 | 一种ω-转氨酶的应用及生物酶法去消旋化制备L-草铵膦的方法 |
-
2022
- 2022-12-29 WO PCT/CN2022/143241 patent/WO2023125769A1/zh unknown
- 2022-12-29 AR ARP220103649A patent/AR128162A1/es unknown
- 2022-12-30 TW TW111150907A patent/TW202334407A/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023125769A1 (zh) | 2023-07-06 |
| TW202334407A (zh) | 2023-09-01 |
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