MX2020005668A - Metodo de cultivo de celulas. - Google Patents

Metodo de cultivo de celulas.

Info

Publication number
MX2020005668A
MX2020005668A MX2020005668A MX2020005668A MX2020005668A MX 2020005668 A MX2020005668 A MX 2020005668A MX 2020005668 A MX2020005668 A MX 2020005668A MX 2020005668 A MX2020005668 A MX 2020005668A MX 2020005668 A MX2020005668 A MX 2020005668A
Authority
MX
Mexico
Prior art keywords
neural crest
crest cells
cells
gsk3î2
î1⁄4m
Prior art date
Application number
MX2020005668A
Other languages
English (en)
Inventor
makoto Ikeya
Yayoi Kamiya
Original Assignee
Univ Kyoto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kyoto filed Critical Univ Kyoto
Publication of MX2020005668A publication Critical patent/MX2020005668A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0626—Melanocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Abstract

Como una técnica para proliferar células de la cresta neural sin reducir la capacidad de diferenciación, se proporciona un método para producir células de la cresta neural, que comprende los pasos de: (1) obtener células de la cresta neural; y (2) cultivar en suspensión las células de la cresta neural en un medio que comprende un inhibidor de GSK3ß y un factor básico de crecimiento de fibroblastos (bFGF), en donde el medio comprende el inhibidor de GSK3ß con una concentración que exhibe un efecto equivalente a aquel exhibido por CHIR99021 con una concentración mayor que 1 µM y menor que 5 µM.
MX2020005668A 2017-11-30 2018-11-29 Metodo de cultivo de celulas. MX2020005668A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017230074 2017-11-30
PCT/JP2018/043949 WO2019107485A1 (ja) 2017-11-30 2018-11-29 細胞の培養方法

Publications (1)

Publication Number Publication Date
MX2020005668A true MX2020005668A (es) 2020-11-24

Family

ID=66665610

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020005668A MX2020005668A (es) 2017-11-30 2018-11-29 Metodo de cultivo de celulas.

Country Status (15)

Country Link
US (1) US11959100B2 (es)
EP (1) EP3719120A4 (es)
JP (2) JP7330466B2 (es)
KR (1) KR20200091885A (es)
CN (1) CN111492052A (es)
AU (1) AU2018376391A1 (es)
BR (1) BR112020010539A2 (es)
CA (1) CA3083253A1 (es)
CO (1) CO2020007772A2 (es)
EA (1) EA202091354A1 (es)
IL (1) IL274746A (es)
MX (1) MX2020005668A (es)
SG (1) SG11202004964WA (es)
TW (1) TW201940693A (es)
WO (1) WO2019107485A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210198624A1 (en) * 2018-08-22 2021-07-01 Kyoto University Method for producing enteric neural precursors
EP4353243A1 (en) * 2021-06-10 2024-04-17 Ajinomoto Co., Inc. Method for producing mesenchymal stem cells
WO2023106122A1 (ja) * 2021-12-06 2023-06-15 国立大学法人京都大学 間葉系譜への分化に特化した神経堤細胞の製造方法

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ226750A (en) 1987-10-29 1990-09-26 Amrad Corp Ltd Immortalisation of neural precursor cells by introducing a retrovirus vector containing a myc-oncogene
EP1025205A2 (en) 1997-10-28 2000-08-09 President And Fellows Of Harvard College (in vitro) differentiation of vascular smooth muscle cells, methods and reagents related thereto
US20030003572A1 (en) 1999-03-05 2003-01-02 David J. Anderson Isolation and enrichment of neural stem cells from uncultured tissue based on cell-surface marker expression
US6548059B1 (en) 1999-07-22 2003-04-15 The Schepens Eye Research Institute, Inc. Promotion of proliferation of adult corneal endothelial cells
US20030134413A1 (en) 2000-01-14 2003-07-17 Rathjen Peter David Cell production
AUPR349501A0 (en) 2001-03-02 2001-03-29 Bresagen Limited Cellular production control
US7971593B2 (en) 2004-04-10 2011-07-05 Henkel Kgaa Hair rollers
US20060236415A1 (en) 2005-03-09 2006-10-19 Silversides David W Neural crest cells specific promoters; isolated neural crest cells; and methods of isolating and of using same
US8030072B2 (en) 2005-03-15 2011-10-04 Newcastle University Method of isolating epidermal neural crest stem cells
US20070258957A1 (en) 2006-05-04 2007-11-08 Russell Bowermaster Method for obtaining and storing multipotent stem cells
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
EP1999249B8 (en) 2006-03-30 2012-02-15 The University Court Of The University of Edinburgh Culture medium containing kinase inhibitors. and uses thereof
WO2008018190A1 (fr) 2006-08-09 2008-02-14 Biomaster, Inc. Cellules de la crête neurale dérivées de tissu adipeux
JP2008099662A (ja) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
EP2079831A2 (en) 2006-11-07 2009-07-22 Keck Graduate Institute Enriched stem cell and progenitor cell populations, and methods of producing and using such populations
US7661738B2 (en) 2006-11-28 2010-02-16 Veritainer Corporation Radiation detection unit for mounting a radiation sensor to a container crane
JP2010523117A (ja) 2007-04-07 2010-07-15 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 体細胞の再プログラミング
JP2010528622A (ja) 2007-05-30 2010-08-26 ザ ジェネラル ホスピタル コーポレイション 体細胞から多能性細胞を生成する方法
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
WO2009155301A2 (en) 2008-06-17 2009-12-23 The Mclean Hospital Corporation Multipotent neural cells
SG172160A1 (en) 2008-12-17 2011-07-28 Scripps Research Inst Generation and maintenance of stem cells
GB201111244D0 (en) 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
ES2948761T3 (es) 2009-02-03 2023-09-18 Koninklijke Nederlandse Akademie Van Wetenschappen Combinaciones de inhibidores de la replicación del virus de la influenza
WO2010108008A2 (en) 2009-03-18 2010-09-23 University Of Georgia Research Foundation Bsc cell differentiation and use in therapy
WO2010140698A1 (ja) 2009-06-03 2010-12-09 国立大学法人東北大学 多能性幹細胞からの神経堤細胞群の分化誘導方法
US20120219535A1 (en) 2009-10-02 2012-08-30 University Of Southern California Cranial neural crest stem cells and culture condition that supports their growth
WO2011144901A1 (en) 2010-05-20 2011-11-24 The University Of Newcastle Upon Tyne Expansion and directed differentiation of epidermal neural crest stem cells
WO2012091978A2 (en) 2010-12-31 2012-07-05 University Of Georgia Research Foundation, Inc. Differentiation of human pluripotent stem cells to multipotent neural crest cells
US20140093960A1 (en) 2011-05-19 2014-04-03 The University Of Tokushima Cell differentiation inducer and differentiation inducing method
WO2012164137A1 (es) 2011-05-30 2012-12-06 Fundación Investigación En Regeneración Del Sistema Nervioso Células madre y células de la estirpe neural derivadas de la glía envolvente olfatoria, y sus aplicaciones
JP6041270B2 (ja) 2011-10-06 2016-12-07 学校法人慶應義塾 角膜内皮細胞の製造方法
US10472607B2 (en) 2011-12-14 2019-11-12 National Chung Hsing University Culture medium and method for inducing differentiation of pluripotent stem cells into neuroepithelial cells
EP2614829A1 (en) 2012-01-11 2013-07-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mammalian neural plate border stem cells capable of forming neural tube and neural crest cell lineages including central and peripheral neurons
CN102604894B (zh) 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
WO2013131012A1 (en) 2012-03-01 2013-09-06 University Of Miami Isolation and use of pluripotent stem cell population from adult neural crest-derived tissues
KR101389851B1 (ko) 2012-05-04 2014-04-29 이화여자대학교 산학협력단 신경능선줄기세포의 배양방법 및 그 용도
US9341625B2 (en) 2012-05-16 2016-05-17 Becton, Dickinson And Company Cell-surface signatures for isolating neurons from cell cultures derived from pluripotent stem cells
CN102952777B (zh) 2012-11-29 2015-01-21 山东大学 人胚胎干细胞定向分化为角膜内皮细胞的诱导方法
US20140248696A1 (en) 2013-03-01 2014-09-04 Wisconsin Alumni Research Foundation Methods of maintaining, expanding, and diffrentiating neuronal subtype specific progenitors
KR101445026B1 (ko) 2013-03-14 2014-09-26 건국대학교 산학협력단 낭배외피줄기세포의 신경세포로의 분화 유도 방법
ES2831424T3 (es) 2013-04-23 2021-06-08 Yeda Res & Dev Células madre pluripotentes naif aisladas y métodos para generarlas
BR112015032235A2 (pt) 2013-07-23 2017-07-25 Hoffmann La Roche método de produção de células da crista neural, células da crista neural, células de schwann, condrócitos, células musculares lisas ou adipócitos, biobanco de células da crista neural, uso das células, composição terapêutica e métodos e usos
EP3044310A4 (en) 2013-09-13 2017-06-21 University Health Network Methods and compositions for generating epicardium cells
WO2015042356A1 (en) 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
US10786536B2 (en) 2013-10-29 2020-09-29 Vestion, Inc. Cardiac neural crest cells and methods of use thereof
KR101743799B1 (ko) 2013-11-25 2017-06-07 단국대학교 천안캠퍼스 산학협력단 아스코르브산을 이용하여 인간 역분화 줄기세포를 신경능선 세포로 분화시키는 방법 및 상기 방법에 의해 형성된 신경능선 세포
SG10201500884WA (en) 2014-02-05 2015-09-29 Agency Science Tech & Res Manufacture Of Vascular Smooth Muscle Cells And The Use
JP6304818B2 (ja) 2014-04-21 2018-04-04 花王株式会社 皮膚由来多能性前駆細胞の作製方法
CN105154386B (zh) 2014-05-30 2018-04-24 中国人民解放军第二军医大学东方肝胆外科医院 人肝细胞长期维持和增殖传代培养的专用培养基和培养方法
WO2016016894A1 (en) 2014-07-30 2016-02-04 Yeda Research And Development Co. Ltd. Media for culturing pluripotent stem cells
CN105441384B (zh) 2014-09-26 2021-01-05 北京大学 一种制备动物和人类原始多潜能干细胞的方法，试剂盒及用途
WO2016099602A1 (en) 2014-12-18 2016-06-23 University Of Utah Research Foundation Induced pluripotent stem cell and method for producing the same
WO2016103269A1 (en) 2014-12-23 2016-06-30 Ramot At Tel-Aviv University Ltd. Populations of neural progenitor cells and methods of producing and using same
JP6673586B2 (ja) * 2014-12-24 2020-03-25 国立大学法人京都大学 異所性骨化の予防・治療剤及びそのスクリーニング方法
KR102121647B1 (ko) 2015-01-15 2020-06-10 고꾸리쯔 다이가꾸 호우징 오사까 다이가꾸 다능성 줄기세포로부터의 각막 상피 세포의 분화 유도 방법
WO2016179243A1 (en) 2015-05-05 2016-11-10 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Reversion of primed pluripotent stem cells to naive pluripotent stem cells
US10443043B2 (en) 2015-05-07 2019-10-15 New York University Methods for making induced pluripotent stem cells
JP6593811B2 (ja) * 2015-06-02 2019-10-23 国立研究開発法人産業技術総合研究所 神経堤細胞から自律神経系の細胞への分化誘導方法
JP6757980B2 (ja) 2015-06-29 2020-09-23 国立大学法人京都大学 多能性幹細胞から生殖細胞への分化誘導方法
LU92771B1 (en) 2015-07-10 2017-01-30 Univ Luxembourg Long-term self-renewing neural stem cells
CN105255826B (zh) 2015-11-27 2019-01-08 中山大学 人iPS细胞向睾丸间质细胞的诱导分化方法及其用途
WO2017099766A1 (en) 2015-12-09 2017-06-15 Intel IP Corporation Aggregated signaling for machine type communication (mtc) devices
CN105483080A (zh) 2015-12-15 2016-04-13 同济大学 一种大鼠胚胎干细胞高效培养基
CN106244522A (zh) 2016-08-12 2016-12-21 浙江译美生物科技有限公司 一种干细胞培养体系及其培养方法
CN106867962B (zh) 2017-03-28 2020-09-04 周婧 一种诱导神经嵴干细胞向色素细胞分化的方法
US20210198624A1 (en) 2018-08-22 2021-07-01 Kyoto University Method for producing enteric neural precursors

Also Published As

Publication number Publication date
EP3719120A1 (en) 2020-10-07
JP7330466B2 (ja) 2023-08-22
CA3083253A1 (en) 2019-06-06
EP3719120A4 (en) 2021-08-11
CO2020007772A2 (es) 2020-08-31
JP2023093693A (ja) 2023-07-04
IL274746A (en) 2020-07-30
KR20200091885A (ko) 2020-07-31
CN111492052A (zh) 2020-08-04
US20210002608A1 (en) 2021-01-07
BR112020010539A2 (pt) 2020-11-17
EA202091354A1 (ru) 2020-08-20
TW201940693A (zh) 2019-10-16
AU2018376391A1 (en) 2020-06-04
SG11202004964WA (en) 2020-06-29
US11959100B2 (en) 2024-04-16
WO2019107485A1 (ja) 2019-06-06
JPWO2019107485A1 (ja) 2020-11-26

Similar Documents

Publication Publication Date Title
MX2020005668A (es) Metodo de cultivo de celulas.
JP2018526992A5 (es)
MY189592A (en) Method for producing cerebellar progenitor tissue
AU2017254264A1 (en) Method for producing retinal tissue
MY192441A (en) Filamentous fungal biomats, methods of their production and methods of their use
WO2015054526A3 (en) Methods of mammalian retinal stem cell production and applications
MX2017010217A (es) Sistema, aparato y metodo para la produccion de celulas y/o productos de celulas.
MX2020002851A (es) Biorreactor de perfusion y metodos de uso relacionados.
MX2012000354A (es) Metodo para cultivar celulas eucarionticas.
MX2022010521A (es) Metodo para producir linfocitos citoliticos naturales a partir de celulas madre pluripotentes.
WO2015121859A8 (en) Method of qualifying cells
NZ597742A (en) Method of producing a polypeptide or virus of interest in a continuous cell culture
SG10201903910RA (en) Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same
NZ611862A (en) Animal cell culturing method
SG10201902355VA (en) Ultra-high density cell banking methods
MX2018013175A (es) Metodo de purificacion para celulas precursoras pancreaticas derivadas a partir de celulas madre pluripotentes y metodo de amplificacion para ellas.
EA201491785A1 (ru) Способ ферментации
MX369395B (es) Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante.
EA202190582A1 (ru) Способ получения энтеральных нейральных клеток-предшественников
PH12019501779A1 (en) Method and device for producing biogas
BR112016005456A2 (pt) método para produzir um l-aminoácido por um método de fermentação, e, dna isolado
BR112018074315A2 (pt) método para produzir óleo com ácidos graxos saturados reduzidos.
IL289452A (en) A method for producing a culture medium preparation for a suspension culture of infectious cells
WO2015177641A3 (en) Methods of oil production in microorganisms
MX2022012829A (es) Metodo para tratamiento de medio antes de inoculacion.