EA202091354A1 - Способ культивирования клеток - Google Patents

Способ культивирования клеток

Info

Publication number
EA202091354A1
EA202091354A1 EA202091354A EA202091354A EA202091354A1 EA 202091354 A1 EA202091354 A1 EA 202091354A1 EA 202091354 A EA202091354 A EA 202091354A EA 202091354 A EA202091354 A EA 202091354A EA 202091354 A1 EA202091354 A1 EA 202091354A1
Authority
EA
Eurasian Patent Office
Prior art keywords
neural crest
crest cells
cells
gsk3 inhibitor
cultivation
Prior art date
Application number
EA202091354A
Other languages
English (en)
Inventor
Макото Икея
Яей Камия
Original Assignee
Киото Юниверсити
Такеда Фармасьютикал Компани Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Киото Юниверсити, Такеда Фармасьютикал Компани Лимитед filed Critical Киото Юниверсити
Publication of EA202091354A1 publication Critical patent/EA202091354A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0626—Melanocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

В качестве способа обеспечения пролиферации клеток нервного гребня без уменьшения способности к дифференцировке предусматривается способ получения клеток нервного гребня, включающий стадии (1) получения клеток нервного гребня и (2) культивирования с использованием суспензионной культуры клеток нервного гребня в среде, содержащей ингибитор GSK3 и основной фибробластный фактор роста (bFGF), где среда содержит ингибитор GSK3 в концентрации, который демонстрирует эффект, эквивалентный эффекту, который демонстрирует CHIR99021 в концентрации выше 1 и ниже 5 мкМ.
EA202091354A 2017-11-30 2018-11-29 Способ культивирования клеток EA202091354A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017230074 2017-11-30
PCT/JP2018/043949 WO2019107485A1 (ja) 2017-11-30 2018-11-29 細胞の培養方法

Publications (1)

Publication Number Publication Date
EA202091354A1 true EA202091354A1 (ru) 2020-08-20

Family

ID=66665610

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202091354A EA202091354A1 (ru) 2017-11-30 2018-11-29 Способ культивирования клеток

Country Status (15)

Country Link
US (1) US11959100B2 (ru)
EP (1) EP3719120A4 (ru)
JP (2) JP7330466B2 (ru)
KR (1) KR20200091885A (ru)
CN (1) CN111492052A (ru)
AU (1) AU2018376391A1 (ru)
BR (1) BR112020010539A2 (ru)
CA (1) CA3083253A1 (ru)
CO (1) CO2020007772A2 (ru)
EA (1) EA202091354A1 (ru)
IL (1) IL274746A (ru)
MX (1) MX2020005668A (ru)
SG (1) SG11202004964WA (ru)
TW (1) TW201940693A (ru)
WO (1) WO2019107485A1 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3842525A4 (en) * 2018-08-22 2022-07-27 Kyoto University PROCEDURE FOR GENERATING A NERVE PROGENIOR CELL OF THE INTESTINAL TRACT
EP4353243A1 (en) * 2021-06-10 2024-04-17 Ajinomoto Co., Inc. Method for producing mesenchymal stem cells
WO2023106122A1 (ja) * 2021-12-06 2023-06-15 国立大学法人京都大学 間葉系譜への分化に特化した神経堤細胞の製造方法

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ226750A (en) 1987-10-29 1990-09-26 Amrad Corp Ltd Immortalisation of neural precursor cells by introducing a retrovirus vector containing a myc-oncogene
WO1999021965A2 (en) 1997-10-28 1999-05-06 President And Fellows Of Harvard College In vitro differentiation of vascular smooth muscle cells, methods and reagents related thereto
US20030003572A1 (en) 1999-03-05 2003-01-02 David J. Anderson Isolation and enrichment of neural stem cells from uncultured tissue based on cell-surface marker expression
US6548059B1 (en) 1999-07-22 2003-04-15 The Schepens Eye Research Institute, Inc. Promotion of proliferation of adult corneal endothelial cells
US20030134413A1 (en) 2000-01-14 2003-07-17 Rathjen Peter David Cell production
AUPR349501A0 (en) 2001-03-02 2001-03-29 Bresagen Limited Cellular production control
DK1610642T3 (da) 2004-04-10 2006-11-27 Henkel Kgaa Curler
US20060236415A1 (en) 2005-03-09 2006-10-19 Silversides David W Neural crest cells specific promoters; isolated neural crest cells; and methods of isolating and of using same
US8030072B2 (en) 2005-03-15 2011-10-04 Newcastle University Method of isolating epidermal neural crest stem cells
US20070258957A1 (en) 2006-05-04 2007-11-08 Russell Bowermaster Method for obtaining and storing multipotent stem cells
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
GB2436737B (en) 2006-03-30 2008-07-09 Univ Edinburgh Culture medium containing kinase inhibitors,and uses thereof
WO2008018190A1 (fr) 2006-08-09 2008-02-14 Biomaster, Inc. Cellules de la crête neurale dérivées de tissu adipeux
JP2008099662A (ja) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
EP2079831A2 (en) 2006-11-07 2009-07-22 Keck Graduate Institute Enriched stem cell and progenitor cell populations, and methods of producing and using such populations
US7661738B2 (en) 2006-11-28 2010-02-16 Veritainer Corporation Radiation detection unit for mounting a radiation sensor to a container crane
EP3878949A1 (en) 2007-04-07 2021-09-15 Whitehead Institute for Biomedical Research Reprogramming of somatic cells
EP2164951A2 (en) 2007-05-30 2010-03-24 The General Hospital Corporation Methods of generating pluripotent cells from somatic cells
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
WO2009155301A2 (en) 2008-06-17 2009-12-23 The Mclean Hospital Corporation Multipotent neural cells
ES2645869T3 (es) 2008-12-17 2017-12-11 The Scripps Research Institute Generación y mantenimiento de células madre
MX2011008044A (es) 2009-02-03 2012-01-12 Koninklijke Nederlandse Akademie Van Wetenschappen Medio de cultivo para celulas madre epiteliales y organoides que comprenden las celulas madre.
GB201111244D0 (en) 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
WO2010108008A2 (en) 2009-03-18 2010-09-23 University Of Georgia Research Foundation Bsc cell differentiation and use in therapy
WO2010140698A1 (ja) 2009-06-03 2010-12-09 国立大学法人東北大学 多能性幹細胞からの神経堤細胞群の分化誘導方法
WO2011041062A1 (en) 2009-10-02 2011-04-07 University Of Southern California Cranial neural crest stem cells and culture condition that supports their growth
WO2011144901A1 (en) 2010-05-20 2011-11-24 The University Of Newcastle Upon Tyne Expansion and directed differentiation of epidermal neural crest stem cells
WO2012091978A2 (en) 2010-12-31 2012-07-05 University Of Georgia Research Foundation, Inc. Differentiation of human pluripotent stem cells to multipotent neural crest cells
CN103562379A (zh) 2011-05-19 2014-02-05 国立大学法人德岛大学 细胞分化诱导剂以及分化诱导方法
WO2012164137A1 (es) 2011-05-30 2012-12-06 Fundación Investigación En Regeneración Del Sistema Nervioso Células madre y células de la estirpe neural derivadas de la glía envolvente olfatoria, y sus aplicaciones
WO2013051722A1 (ja) 2011-10-06 2013-04-11 学校法人 慶應義塾 角膜内皮細胞の製造方法
US10472607B2 (en) 2011-12-14 2019-11-12 National Chung Hsing University Culture medium and method for inducing differentiation of pluripotent stem cells into neuroepithelial cells
EP2614829A1 (en) 2012-01-11 2013-07-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mammalian neural plate border stem cells capable of forming neural tube and neural crest cell lineages including central and peripheral neurons
CN102604894B (zh) 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
WO2013131012A1 (en) 2012-03-01 2013-09-06 University Of Miami Isolation and use of pluripotent stem cell population from adult neural crest-derived tissues
KR101389851B1 (ko) 2012-05-04 2014-04-29 이화여자대학교 산학협력단 신경능선줄기세포의 배양방법 및 그 용도
CN104471073B (zh) 2012-05-16 2017-03-01 贝克顿·迪金森公司 用于从衍生自多能干细胞的细胞培养物中分离神经元的细胞表面特征物
CN102952777B (zh) 2012-11-29 2015-01-21 山东大学 人胚胎干细胞定向分化为角膜内皮细胞的诱导方法
US20140248696A1 (en) 2013-03-01 2014-09-04 Wisconsin Alumni Research Foundation Methods of maintaining, expanding, and diffrentiating neuronal subtype specific progenitors
KR101445026B1 (ko) 2013-03-14 2014-09-26 건국대학교 산학협력단 낭배외피줄기세포의 신경세포로의 분화 유도 방법
US10920192B2 (en) 2013-04-23 2021-02-16 Yeda Research And Development Co. Ltd. Isolated naive pluripotent stem cells and methods of generating same
CA2914520A1 (en) 2013-07-23 2015-01-29 F. Hoffmann-La Roche Ag Small molecule based conversion of somatic cells into neural crest cells
NZ716860A (en) 2013-09-13 2022-07-01 Univ Health Network Methods and compositions for generating epicardium cells
WO2015042356A1 (en) 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
WO2015066197A2 (en) 2013-10-29 2015-05-07 Vestion, Inc. Cardiac neural crest cells and methods of use thereof
KR101743799B1 (ko) 2013-11-25 2017-06-07 단국대학교 천안캠퍼스 산학협력단 아스코르브산을 이용하여 인간 역분화 줄기세포를 신경능선 세포로 분화시키는 방법 및 상기 방법에 의해 형성된 신경능선 세포
US20150218523A1 (en) 2014-02-05 2015-08-06 Agency For Science, Technology And Research Manufacture of vascular smooth muscle cells and the use
JP6304818B2 (ja) 2014-04-21 2018-04-04 花王株式会社 皮膚由来多能性前駆細胞の作製方法
CN105154386B (zh) 2014-05-30 2018-04-24 中国人民解放军第二军医大学东方肝胆外科医院 人肝细胞长期维持和增殖传代培养的专用培养基和培养方法
US11920164B2 (en) 2014-07-30 2024-03-05 Yeda Research And Development Co. Ltd. Media for culturing naive human pluripotent stem cells
CN105441384B (zh) 2014-09-26 2021-01-05 北京大学 一种制备动物和人类原始多潜能干细胞的方法，试剂盒及用途
US20170327796A1 (en) 2014-12-18 2017-11-16 University Of Utah Research Foundation Induced pluripotent stem cell and method for producing the same
WO2016103269A1 (en) 2014-12-23 2016-06-30 Ramot At Tel-Aviv University Ltd. Populations of neural progenitor cells and methods of producing and using same
WO2016104574A1 (ja) 2014-12-24 2016-06-30 国立大学法人京都大学 異所性骨化の予防・治療剤及びそのスクリーニング方法
WO2016114285A1 (ja) 2015-01-15 2016-07-21 国立大学法人大阪大学 多能性幹細胞からの角膜上皮細胞の分化誘導
WO2016179243A1 (en) 2015-05-05 2016-11-10 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Reversion of primed pluripotent stem cells to naive pluripotent stem cells
US10443043B2 (en) 2015-05-07 2019-10-15 New York University Methods for making induced pluripotent stem cells
WO2016194522A1 (ja) * 2015-06-02 2016-12-08 国立研究開発法人産業技術総合研究所 神経堤細胞から自律神経系の細胞への分化誘導方法
WO2017002888A1 (ja) 2015-06-29 2017-01-05 国立大学法人京都大学 多能性幹細胞から生殖細胞への分化誘導方法
LU92771B1 (en) 2015-07-10 2017-01-30 Univ Luxembourg Long-term self-renewing neural stem cells
CN105255826B (zh) 2015-11-27 2019-01-08 中山大学 人iPS细胞向睾丸间质细胞的诱导分化方法及其用途
WO2017099766A1 (en) 2015-12-09 2017-06-15 Intel IP Corporation Aggregated signaling for machine type communication (mtc) devices
CN105483080A (zh) 2015-12-15 2016-04-13 同济大学 一种大鼠胚胎干细胞高效培养基
CN106244522A (zh) 2016-08-12 2016-12-21 浙江译美生物科技有限公司 一种干细胞培养体系及其培养方法
CN106867962B (zh) 2017-03-28 2020-09-04 周婧 一种诱导神经嵴干细胞向色素细胞分化的方法
EP3842525A4 (en) 2018-08-22 2022-07-27 Kyoto University PROCEDURE FOR GENERATING A NERVE PROGENIOR CELL OF THE INTESTINAL TRACT

Also Published As

Publication number Publication date
US11959100B2 (en) 2024-04-16
MX2020005668A (es) 2020-11-24
IL274746A (en) 2020-07-30
JPWO2019107485A1 (ja) 2020-11-26
WO2019107485A1 (ja) 2019-06-06
AU2018376391A1 (en) 2020-06-04
JP7330466B2 (ja) 2023-08-22
US20210002608A1 (en) 2021-01-07
EP3719120A4 (en) 2021-08-11
EP3719120A1 (en) 2020-10-07
SG11202004964WA (en) 2020-06-29
BR112020010539A2 (pt) 2020-11-17
CN111492052A (zh) 2020-08-04
TW201940693A (zh) 2019-10-16
CA3083253A1 (en) 2019-06-06
KR20200091885A (ko) 2020-07-31
JP2023093693A (ja) 2023-07-04
CO2020007772A2 (es) 2020-08-31

Similar Documents

Publication Publication Date Title
EA202091354A1 (ru) Способ культивирования клеток
BR112022017216A2 (pt) Método para produzir células exterminadoras naturais a partir de células-tronco pluripotentes
AR117697A2 (es) Medios de cultivo celular
RU2016149126A (ru) Применение малых молекул для увеличения экспрессии mafa в панкреатических эндокринных клетках
PH12016502148A1 (en) Methods of microalgae cultivation for increased resource production
AU2017254264A1 (en) Method for producing retinal tissue
EA201890137A1 (ru) Среда для культивирования клеток с добавлением таурина и способы применения
EA202190326A3 (ru) Не содержащая сыворотки среда для культивирования клеток
EA202091407A1 (ru) Состав бессывороточной среды для культивирования клеток и способы его применения
RU2009115186A (ru) Среда культивирования для стволовых клеток и способ
BR112014031424A2 (pt) tratamento de células pluripotentes
EA201491785A1 (ru) Способ ферментации
EA202190582A1 (ru) Способ получения энтеральных нейральных клеток-предшественников
EA202091349A1 (ru) Способы культивирования клеток
BR112018074315A2 (pt) método para produzir óleo com ácidos graxos saturados reduzidos.
EA201501002A1 (ru) Способ культивирования гепатобластподобных клеток и продукт их культивирования
MX2015017414A (es) Bacteria acetogenica.
BR112018070063A2 (pt) processo de cultivo de células
SG11201806843UA (en) Method for producing activated hepatocyte growth factor (hgf)
BR112015015466A2 (pt) método para cultivar células-tronco pluripotentes
EP4235175A3 (en) Methods for culturing human keratinocytes
WO2015177641A3 (en) Methods of oil production in microorganisms
MA32410B1 (fr) Milieux et procede de culture d'algues
EA201690684A1 (ru) Способ осветления выросшей клеточной массы неочищенной культуры клеток с высокой плотностью клеток
JP2016063801A5 (ru)