EA202091354A1 - Способ культивирования клеток - Google Patents

Способ культивирования клеток

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Publication number
EA202091354A1
EA202091354A1 EA202091354A EA202091354A EA202091354A1 EA 202091354 A1 EA202091354 A1 EA 202091354A1 EA 202091354 A EA202091354 A EA 202091354A EA 202091354 A EA202091354 A EA 202091354A EA 202091354 A1 EA202091354 A1 EA 202091354A1
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EA
Eurasian Patent Office
Prior art keywords
neural crest
crest cells
cells
gsk3 inhibitor
cultivation
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EA202091354A
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English (en)
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Макото Икея
Яей Камия
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Киото Юниверсити
Такеда Фармасьютикал Компани Лимитед
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Publication of EA202091354A1 publication Critical patent/EA202091354A1/ru

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Abstract

В качестве способа обеспечения пролиферации клеток нервного гребня без уменьшения способности к дифференцировке предусматривается способ получения клеток нервного гребня, включающий стадии (1) получения клеток нервного гребня и (2) культивирования с использованием суспензионной культуры клеток нервного гребня в среде, содержащей ингибитор GSK3 и основной фибробластный фактор роста (bFGF), где среда содержит ингибитор GSK3 в концентрации, который демонстрирует эффект, эквивалентный эффекту, который демонстрирует CHIR99021 в концентрации выше 1 и ниже 5 мкМ.
EA202091354A 2017-11-30 2018-11-29 Способ культивирования клеток EA202091354A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017230074 2017-11-30
PCT/JP2018/043949 WO2019107485A1 (ja) 2017-11-30 2018-11-29 細胞の培養方法

Publications (1)

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EA202091354A1 true EA202091354A1 (ru) 2020-08-20

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US (1) US11959100B2 (ru)
EP (1) EP3719120A4 (ru)
JP (2) JP7330466B2 (ru)
KR (1) KR20200091885A (ru)
CN (1) CN111492052A (ru)
AU (1) AU2018376391A1 (ru)
BR (1) BR112020010539A2 (ru)
CA (1) CA3083253A1 (ru)
CO (1) CO2020007772A2 (ru)
EA (1) EA202091354A1 (ru)
IL (1) IL274746A (ru)
MX (1) MX2020005668A (ru)
SG (1) SG11202004964WA (ru)
TW (1) TW201940693A (ru)
WO (1) WO2019107485A1 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020040166A1 (ja) * 2018-08-22 2020-02-27 国立大学法人京都大学 腸管神経前駆細胞の製造方法
WO2022259721A1 (ja) * 2021-06-10 2022-12-15 味の素株式会社 間葉系幹細胞の製造方法
WO2023106122A1 (ja) * 2021-12-06 2023-06-15 国立大学法人京都大学 間葉系譜への分化に特化した神経堤細胞の製造方法

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ226750A (en) 1987-10-29 1990-09-26 Amrad Corp Ltd Immortalisation of neural precursor cells by introducing a retrovirus vector containing a myc-oncogene
EP1025205A2 (en) 1997-10-28 2000-08-09 President And Fellows Of Harvard College (in vitro) differentiation of vascular smooth muscle cells, methods and reagents related thereto
US20030003572A1 (en) 1999-03-05 2003-01-02 David J. Anderson Isolation and enrichment of neural stem cells from uncultured tissue based on cell-surface marker expression
US6548059B1 (en) 1999-07-22 2003-04-15 The Schepens Eye Research Institute, Inc. Promotion of proliferation of adult corneal endothelial cells
US20030134413A1 (en) 2000-01-14 2003-07-17 Rathjen Peter David Cell production
AUPR349501A0 (en) 2001-03-02 2001-03-29 Bresagen Limited Cellular production control
EP1610642B1 (de) 2004-04-10 2006-08-02 Henkel Kommanditgesellschaft auf Aktien Lockenwickler
US20060236415A1 (en) 2005-03-09 2006-10-19 Silversides David W Neural crest cells specific promoters; isolated neural crest cells; and methods of isolating and of using same
US8030072B2 (en) 2005-03-15 2011-10-04 Newcastle University Method of isolating epidermal neural crest stem cells
US20070258957A1 (en) 2006-05-04 2007-11-08 Russell Bowermaster Method for obtaining and storing multipotent stem cells
CN101864392B (zh) 2005-12-13 2016-03-23 国立大学法人京都大学 核重新编程因子
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
AU2007232393A1 (en) 2006-03-30 2007-10-11 The University Court Of The University Of Edinburgh Culture medium containing kinase inhibitors. and uses thereof
WO2008018190A1 (fr) 2006-08-09 2008-02-14 Biomaster, Inc. Cellules de la crête neurale dérivées de tissu adipeux
JP2008099662A (ja) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
EP2079831A2 (en) 2006-11-07 2009-07-22 Keck Graduate Institute Enriched stem cell and progenitor cell populations, and methods of producing and using such populations
US7661738B2 (en) 2006-11-28 2010-02-16 Veritainer Corporation Radiation detection unit for mounting a radiation sensor to a container crane
CA3071055A1 (en) 2007-04-07 2008-10-16 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
EP2164951A2 (en) 2007-05-30 2010-03-24 The General Hospital Corporation Methods of generating pluripotent cells from somatic cells
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
WO2009155301A2 (en) 2008-06-17 2009-12-23 The Mclean Hospital Corporation Multipotent neural cells
CN102317442B (zh) 2008-12-17 2014-08-13 斯克里普斯研究所 干细胞的产生和保持
GB201111244D0 (en) 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
RU2555545C2 (ru) 2009-02-03 2015-07-10 Конинклейке Недерландсе Академи Ван Ветенсаппен Культуральная среда для эпителиальных стволовых клеток и органоидов, содержащих указанные стволовые клетки
WO2010108008A2 (en) 2009-03-18 2010-09-23 University Of Georgia Research Foundation Bsc cell differentiation and use in therapy
JP5700301B2 (ja) 2009-06-03 2015-04-15 国立大学法人大阪大学 多能性幹細胞からの神経堤細胞群の分化誘導方法
WO2011041062A1 (en) 2009-10-02 2011-04-07 University Of Southern California Cranial neural crest stem cells and culture condition that supports their growth
WO2011144901A1 (en) 2010-05-20 2011-11-24 The University Of Newcastle Upon Tyne Expansion and directed differentiation of epidermal neural crest stem cells
US20130280804A1 (en) 2010-12-31 2013-10-24 Stephen Dalton Differentiation of human pluripotent stem cells to multipotent neural crest cells
US20140093960A1 (en) 2011-05-19 2014-04-03 The University Of Tokushima Cell differentiation inducer and differentiation inducing method
WO2012164137A1 (es) 2011-05-30 2012-12-06 Fundación Investigación En Regeneración Del Sistema Nervioso Células madre y células de la estirpe neural derivadas de la glía envolvente olfatoria, y sus aplicaciones
US9347042B2 (en) 2011-10-06 2016-05-24 Keio University Method for producing corneal endothelial cell
US10472607B2 (en) 2011-12-14 2019-11-12 National Chung Hsing University Culture medium and method for inducing differentiation of pluripotent stem cells into neuroepithelial cells
EP2614829A1 (en) 2012-01-11 2013-07-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mammalian neural plate border stem cells capable of forming neural tube and neural crest cell lineages including central and peripheral neurons
CN102604894B (zh) 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
US10106773B2 (en) 2012-03-01 2018-10-23 University Of Miami Isolation and use of pluripotent stem cell population from adult neural crest-derived tissues
KR101389851B1 (ko) 2012-05-04 2014-04-29 이화여자대학교 산학협력단 신경능선줄기세포의 배양방법 및 그 용도
ES2666503T3 (es) 2012-05-16 2018-05-04 Becton, Dickinson And Company Características distintivas de superficie celular para aislar neuronas de cultivos celulares derivados de células madre pluripotentes
CN102952777B (zh) 2012-11-29 2015-01-21 山东大学 人胚胎干细胞定向分化为角膜内皮细胞的诱导方法
US20140248696A1 (en) 2013-03-01 2014-09-04 Wisconsin Alumni Research Foundation Methods of maintaining, expanding, and diffrentiating neuronal subtype specific progenitors
KR101445026B1 (ko) 2013-03-14 2014-09-26 건국대학교 산학협력단 낭배외피줄기세포의 신경세포로의 분화 유도 방법
WO2014174470A1 (en) 2013-04-23 2014-10-30 Yeda Research And Development Co. Ltd. Isolated naive pluripotent stem cells and methods of generating same
BR112015032235A2 (pt) 2013-07-23 2017-07-25 Hoffmann La Roche método de produção de células da crista neural, células da crista neural, células de schwann, condrócitos, células musculares lisas ou adipócitos, biobanco de células da crista neural, uso das células, composição terapêutica e métodos e usos
SG11201601922PA (en) 2013-09-13 2016-04-28 Univ Health Network Methods and compositions for generating epicardium cells
US20160230143A1 (en) 2013-09-19 2016-08-11 The U.S.A., As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
JP2016537414A (ja) 2013-10-29 2016-12-01 ベスティオン、インク． 心臓神経堤細胞、及びその使用方法
KR101743799B1 (ko) 2013-11-25 2017-06-07 단국대학교 천안캠퍼스 산학협력단 아스코르브산을 이용하여 인간 역분화 줄기세포를 신경능선 세포로 분화시키는 방법 및 상기 방법에 의해 형성된 신경능선 세포
SG10201806695QA (en) 2014-02-05 2018-09-27 Agency Science Tech & Res Manufacture Of Vascular Smooth Muscle Cells And The Use
JP6304818B2 (ja) 2014-04-21 2018-04-04 花王株式会社 皮膚由来多能性前駆細胞の作製方法
CN105154386B (zh) 2014-05-30 2018-04-24 中国人民解放军第二军医大学东方肝胆外科医院 人肝细胞长期维持和增殖传代培养的专用培养基和培养方法
EP3194572B1 (en) 2014-07-30 2023-10-25 Yeda Research and Development Co. Ltd. Media for culturing pluripotent stem cells
CN105441384B (zh) 2014-09-26 2021-01-05 北京大学 一种制备动物和人类原始多潜能干细胞的方法，试剂盒及用途
US20170327796A1 (en) 2014-12-18 2017-11-16 University Of Utah Research Foundation Induced pluripotent stem cell and method for producing the same
WO2016103269A1 (en) 2014-12-23 2016-06-30 Ramot At Tel-Aviv University Ltd. Populations of neural progenitor cells and methods of producing and using same
JP6673586B2 (ja) 2014-12-24 2020-03-25 国立大学法人京都大学 異所性骨化の予防・治療剤及びそのスクリーニング方法
WO2016114285A1 (ja) 2015-01-15 2016-07-21 国立大学法人大阪大学 多能性幹細胞からの角膜上皮細胞の分化誘導
EP3292198A4 (en) 2015-05-05 2018-10-17 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Reversion of primed pluripotent stem cells to naive pluripotent stem cells
US10443043B2 (en) 2015-05-07 2019-10-15 New York University Methods for making induced pluripotent stem cells
WO2016194522A1 (ja) * 2015-06-02 2016-12-08 国立研究開発法人産業技術総合研究所 神経堤細胞から自律神経系の細胞への分化誘導方法
US10563171B2 (en) 2015-06-29 2020-02-18 Kyoto University Method for inducing differentiation of pluripotent stem cells into germ cells
LU92771B1 (en) 2015-07-10 2017-01-30 Univ Luxembourg Long-term self-renewing neural stem cells
CN105255826B (zh) 2015-11-27 2019-01-08 中山大学 人iPS细胞向睾丸间质细胞的诱导分化方法及其用途
WO2017099766A1 (en) 2015-12-09 2017-06-15 Intel IP Corporation Aggregated signaling for machine type communication (mtc) devices
CN105483080A (zh) 2015-12-15 2016-04-13 同济大学 一种大鼠胚胎干细胞高效培养基
CN106244522A (zh) 2016-08-12 2016-12-21 浙江译美生物科技有限公司 一种干细胞培养体系及其培养方法
CN106867962B (zh) 2017-03-28 2020-09-04 周婧 一种诱导神经嵴干细胞向色素细胞分化的方法
WO2020040166A1 (ja) 2018-08-22 2020-02-27 国立大学法人京都大学 腸管神経前駆細胞の製造方法

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