MX369395B - Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante. - Google Patents
Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante.Info
- Publication number
- MX369395B MX369395B MX2016010236A MX2016010236A MX369395B MX 369395 B MX369395 B MX 369395B MX 2016010236 A MX2016010236 A MX 2016010236A MX 2016010236 A MX2016010236 A MX 2016010236A MX 369395 B MX369395 B MX 369395B
- Authority
- MX
- Mexico
- Prior art keywords
- glycosylation
- cell growth
- medium
- modulation
- conditions
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
La presente invención se refiere a los métodos para seleccionar entre las condiciones que mejoran el crecimiento celular o la generación de biomasa, y las condiciones que afectan la madurez de N-glucosilación de la glucoproteína expresada, producida por células eucarióticas bajo condiciones de cultivo de fermentación, por el ajuste de las concentraciones de hierro, cobre, zinc y manganeso en el medio. De este modo, en los métodos de la presente invención, las células productoras de glucoproteína son cultivadas en un medio que está diseñado a la medida para un resultado final deseado. La invención también abarca el medio y el uso de las mismas.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14157030 | 2014-02-27 | ||
| PCT/EP2015/053804 WO2015128314A1 (en) | 2014-02-27 | 2015-02-24 | Modulation of cell growth and glycosylation in recombinant glycoprotein production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016010236A MX2016010236A (es) | 2016-10-13 |
| MX369395B true MX369395B (es) | 2019-11-07 |
Family
ID=50184787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016010236A MX369395B (es) | 2014-02-27 | 2015-02-24 | Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante. |
Country Status (20)
| Country | Link |
|---|---|
| US (2) | US20170058309A1 (es) |
| EP (1) | EP3110961B1 (es) |
| JP (2) | JP6831702B2 (es) |
| KR (1) | KR102280638B1 (es) |
| CN (1) | CN106133146B (es) |
| BR (1) | BR112016017660B1 (es) |
| CA (1) | CA2937611C (es) |
| DK (1) | DK3110961T3 (es) |
| ES (1) | ES2769003T3 (es) |
| HR (1) | HRP20200100T1 (es) |
| HU (1) | HUE047575T2 (es) |
| LT (1) | LT3110961T (es) |
| MX (1) | MX369395B (es) |
| PL (1) | PL3110961T3 (es) |
| PT (1) | PT3110961T (es) |
| RS (1) | RS59881B1 (es) |
| RU (1) | RU2712562C2 (es) |
| SG (1) | SG11201606856SA (es) |
| SI (1) | SI3110961T1 (es) |
| WO (1) | WO2015128314A1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2856580C (en) | 2011-11-21 | 2019-12-31 | Arthur M. Nonomura | Methods and systems for growing plants using silicate-based substrates, cultivation of enhanced photosynthetic productivity and photosafening by utilization of exogenous glycopyranosides for endogenous glycopyranosyl-protein derivatives, and formulations, processes and systems for the same |
| KR102280638B1 (ko) * | 2014-02-27 | 2021-07-22 | 에프. 호프만-라 로슈 아게 | 재조합 당단백질 제조에서의 세포 성장 및 글리코실화의 조절 |
| EA202091598A3 (ru) | 2014-12-01 | 2021-01-29 | Эмджен Инк. | Процесс контроля уровня содержания гликанов в составе |
| CA3020437A1 (en) | 2016-04-29 | 2017-11-02 | Innovation Hammer Llc | Formulations and methods for treating photosynthetic organisms and enhancing qualities and quantities of yields with glycan composite formulations |
| WO2019077628A1 (en) * | 2017-10-16 | 2019-04-25 | Council Of Scientific & Industrial Research | ZINC SUPPLEMENTATION TO DECREASE GALACTOSYLATION OF RECOMBINANT GLYCOPROTEINS |
| KR20200135781A (ko) * | 2018-03-26 | 2020-12-03 | 암젠 인크 | 세포 배양에서 생산된 항체의 총 비푸코실화 당형태 |
| WO2019224333A1 (en) * | 2018-05-24 | 2019-11-28 | Ares Trading S.A. | Method for controlling the afucosylation level of a glycoprotein composition |
| HUP1800376A2 (hu) | 2018-11-07 | 2020-05-28 | Richter Gedeon Nyrt | Sejttenyészetben elõállított rekombináns glikoprotein glikozilációs-mintázatának megváltoztatására szolgáló módszer |
| JP2022513018A (ja) * | 2018-11-13 | 2022-02-07 | ヤンセン バイオテツク，インコーポレーテツド | 抗ｃｄ３８抗体の生成中の微量金属の制御 |
| JP2020188737A (ja) * | 2019-05-23 | 2020-11-26 | 東ソー株式会社 | 抗体依存性細胞傷害活性が向上した抗体の製造方法 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| IL87737A (en) | 1987-09-11 | 1993-08-18 | Genentech Inc | Method for culturing polypeptide factor dependent vertebrate recombinant cells |
| US5705364A (en) * | 1995-06-06 | 1998-01-06 | Genentech, Inc. | Mammalian cell culture process |
| US6030945A (en) | 1996-01-09 | 2000-02-29 | Genentech, Inc. | Apo-2 ligand |
| EP2243827B2 (en) | 1996-08-30 | 2017-11-22 | Life Technologies Corporation | Serum-free mammalian cell culture medium, and uses thereof |
| EP1092037B1 (en) * | 1998-05-29 | 2005-12-28 | Genentech, Inc. | Cell culture process for producing glycoproteins |
| JP2001120262A (ja) | 1999-10-26 | 2001-05-08 | Welfide Corp | 生理活性物質の産生増強方法 |
| DE60230858D1 (de) | 2001-02-15 | 2009-03-05 | Centocor Inc | Chemisch definiertes medium für kultivierte säugerzellen |
| US20050287666A1 (en) * | 2004-06-29 | 2005-12-29 | Invitrogen Corporation | Cell culture medium comprising transition metals or trace elements |
| US7294484B2 (en) | 2004-08-27 | 2007-11-13 | Wyeth Research Ireland Limited | Production of polypeptides |
| ZA200702434B (en) | 2004-11-02 | 2008-08-27 | Ares Trading Sa | Serum-free cell culture medium for mammalian cells |
| US20070231895A1 (en) | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
| EP2495308A1 (en) | 2005-12-08 | 2012-09-05 | Amgen Inc. | Improved production of glycoproteins using manganese |
| SI2495307T1 (en) | 2006-07-13 | 2018-05-31 | Wyeth Llc | Production of the calibration factor IX with an improved glycolization pattern |
| JP5401319B2 (ja) | 2006-11-03 | 2014-01-29 | ワイス・エルエルシー | 細胞培養における解糖阻害物質 |
| EP2924113B1 (en) | 2007-03-02 | 2019-04-10 | Wyeth LLC | Use of copper and glutamate in cell culture for production of polypeptides |
| US20110053223A1 (en) * | 2009-08-14 | 2011-03-03 | Robert Bayer | Cell culture methods to make antibodies with enhanced adcc function |
| US20120309056A1 (en) * | 2010-02-04 | 2012-12-06 | Leon Arnaud | Fed-batch process using concentrated cell culture medium for the efficient production of biologics in eb66 cells |
| KR101828624B1 (ko) | 2010-04-26 | 2018-02-12 | 노파르티스 아게 | 개선된 세포 배양 방법 |
| WO2012149197A2 (en) | 2011-04-27 | 2012-11-01 | Abbott Laboratories | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| EP2702143B1 (en) | 2011-04-29 | 2018-06-06 | Biocon Research Limited | Methods for reducing accumulation of lactate during culturing and method for producing polypeptide |
| US10059770B2 (en) * | 2012-01-30 | 2018-08-28 | Dr. Reddy's Laboratories Limited | Process of modulating man5 and/or afucosylation content of a glycoprotein composition |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| KR102280638B1 (ko) * | 2014-02-27 | 2021-07-22 | 에프. 호프만-라 로슈 아게 | 재조합 당단백질 제조에서의 세포 성장 및 글리코실화의 조절 |
-
2015
- 2015-02-24 KR KR1020167026630A patent/KR102280638B1/ko active IP Right Grant
- 2015-02-24 CN CN201580010823.5A patent/CN106133146B/zh active Active
- 2015-02-24 RS RS20200090A patent/RS59881B1/sr unknown
- 2015-02-24 PT PT157094301T patent/PT3110961T/pt unknown
- 2015-02-24 WO PCT/EP2015/053804 patent/WO2015128314A1/en active Application Filing
- 2015-02-24 HU HUE15709430A patent/HUE047575T2/hu unknown
- 2015-02-24 BR BR112016017660-0A patent/BR112016017660B1/pt active IP Right Grant
- 2015-02-24 PL PL15709430T patent/PL3110961T3/pl unknown
- 2015-02-24 SI SI201531071T patent/SI3110961T1/sl unknown
- 2015-02-24 RU RU2016138176A patent/RU2712562C2/ru active
- 2015-02-24 MX MX2016010236A patent/MX369395B/es active IP Right Grant
- 2015-02-24 LT LTEP15709430.1T patent/LT3110961T/lt unknown
- 2015-02-24 SG SG11201606856SA patent/SG11201606856SA/en unknown
- 2015-02-24 JP JP2016554322A patent/JP6831702B2/ja active Active
- 2015-02-24 EP EP15709430.1A patent/EP3110961B1/en active Active
- 2015-02-24 CA CA2937611A patent/CA2937611C/en active Active
- 2015-02-24 DK DK15709430.1T patent/DK3110961T3/da active
- 2015-02-24 ES ES15709430T patent/ES2769003T3/es active Active
-
2016
- 2016-08-12 US US15/236,348 patent/US20170058309A1/en not_active Abandoned
-
2019
- 2019-11-27 JP JP2019214046A patent/JP6934507B2/ja active Active
-
2020
- 2020-01-21 HR HRP20200100TT patent/HRP20200100T1/hr unknown
- 2020-08-28 US US17/006,463 patent/US20210222220A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP6934507B2 (ja) | 2021-09-15 |
| WO2015128314A1 (en) | 2015-09-03 |
| CN106133146B (zh) | 2021-05-04 |
| US20170058309A1 (en) | 2017-03-02 |
| HUE047575T2 (hu) | 2020-04-28 |
| SI3110961T1 (sl) | 2020-03-31 |
| DK3110961T3 (da) | 2020-02-03 |
| JP2017506515A (ja) | 2017-03-09 |
| ES2769003T3 (es) | 2020-06-24 |
| KR102280638B1 (ko) | 2021-07-22 |
| BR112016017660B1 (pt) | 2022-04-19 |
| JP6831702B2 (ja) | 2021-02-17 |
| RU2016138176A3 (es) | 2018-10-29 |
| HRP20200100T1 (hr) | 2020-04-03 |
| CA2937611A1 (en) | 2015-09-03 |
| LT3110961T (lt) | 2020-02-10 |
| CA2937611C (en) | 2023-01-03 |
| BR112016017660A2 (es) | 2017-08-08 |
| JP2020054351A (ja) | 2020-04-09 |
| EP3110961A1 (en) | 2017-01-04 |
| PT3110961T (pt) | 2020-01-29 |
| RS59881B1 (sr) | 2020-03-31 |
| SG11201606856SA (en) | 2016-09-29 |
| US20210222220A1 (en) | 2021-07-22 |
| KR20160125514A (ko) | 2016-10-31 |
| RU2712562C2 (ru) | 2020-01-29 |
| EP3110961B1 (en) | 2019-11-27 |
| MX2016010236A (es) | 2016-10-13 |
| CN106133146A (zh) | 2016-11-16 |
| PL3110961T3 (pl) | 2020-04-30 |
| RU2016138176A (ru) | 2018-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2016010236A (es) | Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante. | |
| MX2015016947A (es) | Metodos para producir derivados de acido graso omega-hidroxilados. | |
| MY156588A (en) | Systems using cell culture for production of isoprene | |
| MX2010003620A (es) | Linea de celulas cho-k1. | |
| MX2020000369A (es) | Metodos biologicos para preparar terpenos. | |
| MX2023009109A (es) | Una variante de isopropilmalato sintasa novedosa y un procedimiento de produccion de l-leucina usando la misma. | |
| MX2018005692A (es) | Limitación de trehalosa producida por levaduras en fermentación. | |
| WO2011134919A3 (en) | Cho cells cultivation process 0 | |
| GB2527254A (en) | Benzylisoquinoline alkaloids (BIA) Producing microbes, and methods of making and using the same | |
| MX2018014043A (es) | Composiciones de cultivo celular con antioxidantes y metodos para la produccion de polipeptidos. | |
| MX2020009460A (es) | Disminuir nivel de lactato e incrementar produccion de polipeptidos por regulacion a la baja de la expresion de lactato dehidrogenasa y piruvato dehidrogenasa quinasa. | |
| SG10201810830UA (en) | Cell culture compositions and methods for polypeptide production | |
| EP2438161A4 (en) | PROCESS FOR THE PRODUCTION OF PLURIPOTENT STEM CELLS AND THEIR CULTURE PROCESS | |
| MX2019007765A (es) | Mutantes de algas de productividad elevada que tienen antena fotosintetica reducida. | |
| MX345399B (es) | Metodo de cultivo de celulas animales. | |
| WO2018203947A3 (en) | MODIFIED BIOSYNTHESIS PATHWAYS FOR FERMENTATION TYRAMINE PRODUCTION | |
| MX2020013565A (es) | Produccion microbiana de dioles grasos. | |
| MX2021004099A (es) | Microbioreactor de flujo continuo. | |
| WO2017049240A8 (en) | Selection of pluripotent cells for production of fertile xy female mice | |
| MX2020001357A (es) | Produccion microbiana de aminas grasas. | |
| MX2015011781A (es) | Medio de cultivo celular y metodos de produccion de anticuerpos. | |
| MY191776A (en) | Method for preparing electrocompetent yeast cells, and method for using said cells | |
| MX2018004580A (es) | Metodo para producir proteina de fusion con dominio fc de igg. | |
| MY193587A (en) | Genetically modified microorganism for improved production of fine chemicals on sucrose | |
| MX2016001917A (es) | Metodo para preparar composiciones de harina de microalgas ricas en lipidos con propiedades organolepticas optimizadas. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |