MX2016010236A - Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante. - Google Patents

Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante.

Info

Publication number
MX2016010236A
MX2016010236A MX2016010236A MX2016010236A MX2016010236A MX 2016010236 A MX2016010236 A MX 2016010236A MX 2016010236 A MX2016010236 A MX 2016010236A MX 2016010236 A MX2016010236 A MX 2016010236A MX 2016010236 A MX2016010236 A MX 2016010236A
Authority
MX
Mexico
Prior art keywords
glycosylation
cell growth
medium
modulation
conditions
Prior art date
Application number
MX2016010236A
Other languages
English (en)
Other versions
MX369395B (es
Inventor
Popp Oliver
Beaucamp Nicola
DRABNER Georg
Esslinger Stephanie
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50184787&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2016010236(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2016010236A publication Critical patent/MX2016010236A/es
Publication of MX369395B publication Critical patent/MX369395B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation

Abstract

La presente invención se refiere a los métodos para seleccionar entre las condiciones que mejoran el crecimiento celular o la generación de biomasa, y las condiciones que afectan la madurez de N-glucosilación de la glucoproteína expresada, producida por células eucarióticas bajo condiciones de cultivo de fermentación, por el ajuste de las concentraciones de hierro, cobre, zinc y manganeso en el medio. De este modo, en los métodos de la presente invención, las células productoras de glucoproteína son cultivadas en un medio que está diseñado a la medida para un resultado final deseado. La invención también abarca el medio y el uso de las mismas.
MX2016010236A 2014-02-27 2015-02-24 Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante. MX369395B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14157030 2014-02-27
PCT/EP2015/053804 WO2015128314A1 (en) 2014-02-27 2015-02-24 Modulation of cell growth and glycosylation in recombinant glycoprotein production

Publications (2)

Publication Number Publication Date
MX2016010236A true MX2016010236A (es) 2016-10-13
MX369395B MX369395B (es) 2019-11-07

Family

ID=50184787

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016010236A MX369395B (es) 2014-02-27 2015-02-24 Modulacion del crecimiento celular y glucosilacion en la produccion de glucoproteina recombinante.

Country Status (20)

Country Link
US (2) US20170058309A1 (es)
EP (1) EP3110961B1 (es)
JP (2) JP6831702B2 (es)
KR (1) KR102280638B1 (es)
CN (1) CN106133146B (es)
BR (1) BR112016017660B1 (es)
CA (1) CA2937611C (es)
DK (1) DK3110961T3 (es)
ES (1) ES2769003T3 (es)
HR (1) HRP20200100T1 (es)
HU (1) HUE047575T2 (es)
LT (1) LT3110961T (es)
MX (1) MX369395B (es)
PL (1) PL3110961T3 (es)
PT (1) PT3110961T (es)
RS (1) RS59881B1 (es)
RU (1) RU2712562C2 (es)
SG (1) SG11201606856SA (es)
SI (1) SI3110961T1 (es)
WO (1) WO2015128314A1 (es)

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MX355598B (es) 2011-11-21 2018-04-24 Innovation Hammer Llc Metodos y sistemas para desarrollar plantas usando sustratos basados en silicato, cultivo de productividad fotosintetica mejorada y fotoproteccion mediante la utilizacion de glicopiranosidos exogenos para derivados de glicopiranosil-protein a endogenos, y formulaciones, procesos y sistemas para los mismos.
RU2712562C2 (ru) * 2014-02-27 2020-01-29 Ф.Хоффманн-Ля Рош Аг Модулирование клеточного роста и гликозилирования при производстве рекомбинантных гликопротеинов
WO2016089919A1 (en) 2014-12-01 2016-06-09 Amgen Inc. Process for manipulating the level of glycan content of a glycoprotein
EA201892477A1 (ru) * 2016-04-29 2019-04-30 ИННОВЕЙШН ХАММЕР ЭлЭлСи Препараты и способы для обработки фотосинтезирующих организмов и повышения их качества и урожайности с помощью препаратов композитов гликанов
WO2019077628A1 (en) * 2017-10-16 2019-04-25 Council Of Scientific & Industrial Research ZINC SUPPLEMENTATION TO DECREASE GALACTOSYLATION OF RECOMBINANT GLYCOPROTEINS
EA202092286A1 (ru) * 2018-03-26 2021-03-18 Эмджен Инк. Общие афукозилированные гликоформы антител, полученные в культуре клеток
EP3802846A1 (en) * 2018-05-24 2021-04-14 Ares Trading S.A. Method for controlling the afucosylation level of a glycoprotein composition
HUP1800376A2 (hu) 2018-11-07 2020-05-28 Richter Gedeon Nyrt Sejttenyészetben elõállított rekombináns glikoprotein glikozilációs-mintázatának megváltoztatására szolgáló módszer
BR112021008879A2 (pt) * 2018-11-13 2021-10-26 Janssen Biotech, Inc. Controle de oligometais durante a produção de anticorpos anti-cd38
JP2020188737A (ja) * 2019-05-23 2020-11-26 東ソー株式会社 抗体依存性細胞傷害活性が向上した抗体の製造方法

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IL87737A (en) 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
US5705364A (en) * 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
US6030945A (en) 1996-01-09 2000-02-29 Genentech, Inc. Apo-2 ligand
EP2221361A3 (en) 1996-08-30 2011-02-09 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
ATE314485T1 (de) 1998-05-29 2006-01-15 Genentech Inc Zellkulturverfahren für die produktion von glycoproteinen
JP2001120262A (ja) * 1999-10-26 2001-05-08 Welfide Corp 生理活性物質の産生増強方法
EP1360314B1 (en) 2001-02-15 2009-01-14 Centocor, Inc. Chemically defined medium for cultured mammalian cells
US20050287666A1 (en) 2004-06-29 2005-12-29 Invitrogen Corporation Cell culture medium comprising transition metals or trace elements
TWI384069B (zh) 2004-08-27 2013-02-01 Pfizer Ireland Pharmaceuticals 多胜肽之製法
SI1807504T1 (sl) 2004-11-02 2011-05-31 Ares Trading Sa Brezserumski celiäśni kultivacijski medij za celice sesalcev
US20070231895A1 (en) 2005-11-02 2007-10-04 Lee Gene W Methods for adapting mammalian cells
CA2630811C (en) * 2005-12-08 2016-05-31 Amgen Inc. Improved production of glycoproteins using manganese
CN101541950A (zh) * 2006-07-13 2009-09-23 惠氏公司 糖蛋白的产生
MX2009004519A (es) 2006-11-03 2009-05-12 Wyeth Corp Sustancias que inhiben la glucolisis en un cultivo de celulas.
DK2115126T3 (en) 2007-03-02 2015-05-04 Wyeth Llc Use of copper and glutamate in cell culture for the preparation of polypeptides
US20110053223A1 (en) 2009-08-14 2011-03-03 Robert Bayer Cell culture methods to make antibodies with enhanced adcc function
US20120309056A1 (en) * 2010-02-04 2012-12-06 Leon Arnaud Fed-batch process using concentrated cell culture medium for the efficient production of biologics in eb66 cells
PL3330370T3 (pl) 2010-04-26 2021-09-20 Novartis Ag Sposób hodowania komórek cho
WO2012149197A2 (en) 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
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WO2013114245A1 (en) 2012-01-30 2013-08-08 Dr. Reddy's Laboratories Limited Process of modulating man5 and/or afucosylation content of glycoprotein composition
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RU2712562C2 (ru) * 2014-02-27 2020-01-29 Ф.Хоффманн-Ля Рош Аг Модулирование клеточного роста и гликозилирования при производстве рекомбинантных гликопротеинов

Also Published As

Publication number Publication date
BR112016017660B1 (pt) 2022-04-19
RU2016138176A3 (es) 2018-10-29
US20170058309A1 (en) 2017-03-02
WO2015128314A1 (en) 2015-09-03
RS59881B1 (sr) 2020-03-31
EP3110961A1 (en) 2017-01-04
KR102280638B1 (ko) 2021-07-22
BR112016017660A2 (es) 2017-08-08
CN106133146B (zh) 2021-05-04
SI3110961T1 (sl) 2020-03-31
MX369395B (es) 2019-11-07
LT3110961T (lt) 2020-02-10
HUE047575T2 (hu) 2020-04-28
CA2937611C (en) 2023-01-03
KR20160125514A (ko) 2016-10-31
ES2769003T3 (es) 2020-06-24
EP3110961B1 (en) 2019-11-27
JP6831702B2 (ja) 2021-02-17
RU2712562C2 (ru) 2020-01-29
SG11201606856SA (en) 2016-09-29
DK3110961T3 (da) 2020-02-03
JP2020054351A (ja) 2020-04-09
HRP20200100T1 (hr) 2020-04-03
CN106133146A (zh) 2016-11-16
JP2017506515A (ja) 2017-03-09
JP6934507B2 (ja) 2021-09-15
RU2016138176A (ru) 2018-03-29
PL3110961T3 (pl) 2020-04-30
US20210222220A1 (en) 2021-07-22
PT3110961T (pt) 2020-01-29
CA2937611A1 (en) 2015-09-03

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