KR970701789A - 콜레스테롤의 정량방법(method for quantitatively determining cholesterol) - Google Patents

콜레스테롤의 정량방법(method for quantitatively determining cholesterol)

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Publication number
KR970701789A
KR970701789A KR1019960704881A KR19960704881A KR970701789A KR 970701789 A KR970701789 A KR 970701789A KR 1019960704881 A KR1019960704881 A KR 1019960704881A KR 19960704881 A KR19960704881 A KR 19960704881A KR 970701789 A KR970701789 A KR 970701789A
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cholesterol
lipoprotein
lipoproteins
high specific
substance
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KR1019960704881A
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KR100346658B1 (ko
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코이치 히노
미츠히로 나카무라
미츠히사 마나베
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쿠니요시 이에지
다이이치 가가쿠 야쿠힌 가부시키가이샤
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/825—Pretreatment for removal of interfering factors from sample
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/826—Additives, e.g. buffers, diluents, preservatives
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/104165—Lipid, cholesterol, or triglyceride standard or control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
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    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
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    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

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  • Investigating Or Analysing Biological Materials (AREA)
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Abstract

효소적 측정법에 의해 콜레스테롤을 측정하기 전에 리포단백질을 함유하는 검체에 고비중 리포단백질 이외의 리포단백질과 복합체를 형성하는 물질 및 계면활성제를 첨가함을 특징으로 하는 고비중 리포단백질 중의 콜레스테롤을 정량적으로 측정하는 방법을 개시한다.
본 방법은 원심분리 등의 전처리 필요가 없고, 간편한 조작으로 효율 좋게 HDL 중의 콜레스테롤을 정량할 수 있다. 또한 적은 시료로 간편하게 조작함으로서 특이적인 측정이 가능하기 때문에 각종 자동 분석 장치에 적용할 수 있어서, 임상 검사의 영역에서도 극히 유용하다.

Description

콜레스테롤의 정량방법(METHOD FOR QUANTITATIVELY DETERMINING CHOLESTEROL)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (5)

  1. 리포단백질을 함유하는 검체에 고비중 리포단백질 이외의 리포단백질과 복합체를 형성하는 물질 및 계면 활성제를 첨가한 후, 효소적 측정법에 의해 콜레스테롤을 측정함을 특징으로 하는 고비중 리포단백질중의 콜레스테롤의 정량방법.
  2. 제1항에 있어서, 고비중 리포단백질 이외의 리포단백질과 복합체를 형성하는 물질 및 계면활성제를 첨가한 검체를 직접 콜레스테롤의 측정에 거는 것이 특징인 고비중 리포단백질중의 콜레스테롤의 정량방법.
  3. 제1항 또는 제2항에 있어서, 고비중 리포단백질 이외의 리포단백질과 복합체를 형성하는 물질이 폴리아니온, 2가 금속이온, 수용성 고분자 화합물 또는 고비중 리포단백질 이외의 리포단백질에 대한 항체인 것이 특징인 고비중 리포단백질중의 콜레스테롤의 정량방법.
  4. 제1항 내지 제3항의 어느 1항에 있어서, 계면활성제가 리포단백질을 용해하는 것이 특징인 고비중 리포단백질중의 콜레스테롤의 정량방법.
  5. 제1항 내지 제4항의 어느 1항에 있어서, 콜레스테롤의 측정을 콜레스테롤 에스테라제 및 콜레스테롤 옥시다아제를 조합하여 행하는 것이 특징인 고비중 리포단백질 중의 콜레스테롤의 정량방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960704881A 1995-01-31 1995-04-03 콜레스테롤의정량방법 KR100346658B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP013607/1995 1995-01-31
JP7013607A JP2799835B2 (ja) 1995-01-31 1995-01-31 コレステロールの定量方法
PCT/JP1995/000641 WO1996023902A1 (fr) 1995-01-31 1995-04-03 Procede d'analyse quantitative du cholesterol

Publications (2)

Publication Number Publication Date
KR970701789A true KR970701789A (ko) 1997-04-12
KR100346658B1 KR100346658B1 (ko) 2002-11-23

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US (1) US5773304A (ko)
EP (1) EP0753583B1 (ko)
JP (1) JP2799835B2 (ko)
KR (1) KR100346658B1 (ko)
CN (1) CN1072724C (ko)
AT (1) ATE197070T1 (ko)
AU (1) AU696681B2 (ko)
CA (1) CA2185562A1 (ko)
DE (1) DE69519160T2 (ko)
DK (1) DK0753583T3 (ko)
ES (1) ES2153030T3 (ko)
GR (1) GR3035274T3 (ko)
MX (1) MX9604514A (ko)
PT (1) PT753583E (ko)
TW (1) TW400385B (ko)
WO (1) WO1996023902A1 (ko)

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JPH08201393A (ja) 1996-08-09
MX9604514A (es) 1997-12-31
CN1072724C (zh) 2001-10-10
EP0753583B1 (en) 2000-10-18
DK0753583T3 (da) 2000-11-13
CN1145096A (zh) 1997-03-12
DE69519160T2 (de) 2001-05-17
CA2185562A1 (en) 1996-08-08
EP0753583A1 (en) 1997-01-15
EP0753583A4 (en) 1997-12-10
KR100346658B1 (ko) 2002-11-23
JP2799835B2 (ja) 1998-09-21
WO1996023902A1 (fr) 1996-08-08
ES2153030T3 (es) 2001-02-16
DE69519160D1 (de) 2000-11-23
US5773304A (en) 1998-06-30
GR3035274T3 (en) 2001-04-30
AU696681B2 (en) 1998-09-17
ATE197070T1 (de) 2000-11-15
TW400385B (en) 2000-08-01
AU2085295A (en) 1996-08-21
PT753583E (pt) 2001-03-30

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