KR950702640A - 감염증 진단용 프로브 - Google Patents

감염증 진단용 프로브 Download PDF

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KR950702640A
KR950702640A KR1019950700098A KR19950700098A KR950702640A KR 950702640 A KR950702640 A KR 950702640A KR 1019950700098 A KR1019950700098 A KR 1019950700098A KR 19950700098 A KR19950700098 A KR 19950700098A KR 950702640 A KR950702640 A KR 950702640A
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probe
infectious diseases
dna
diagnosing
infectious
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KR1019950700098A
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KR0159071B1 (ko
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쓰네야 오오노
아끼오 마쓰히사
히로쓰구 우에하라
소오지 에다
Original Assignee
쓰네야 오오노
도다 미끼오
후소야꾸힝 고오교 가부시끼가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

주요한 감염증 질환 원인균으로부터 추출한 DNA를 Hind Ⅲ로 완전 소화하고, 적당한 벡터에 클로닝하여 각기의 균에 특유한 DNA를 함유하는 프로브를 선발함으로써, 감염증 질환의 원인균의 신속한 검출 및 동정에 유용한 프로브를 제공함과 동시에, 선발한 프로브의 염기배열을 해명하고 임상검체에 함유되는 게놈 DNA와의 비교 참조용에 알맞는 표준배열까지도 제공하는 것을 목적으로 한다.

Description

감염증 진단용 프로브
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 황색포도 상구균(Staphylococcus aureus) 검출용 프로브의 Hind Ⅲ 단편의 제한효소 지도이다,
제3도는 장내구균(Enterococcus faecalis; 엔테로코커스 패켈리스) 검출용 프로브의 Hind Ⅲ 단편의 제한효소 지도이다,
제5도는 대장균(Escherichia coli) 검출용 프로브의 Hind Ⅲ 단편의 제한효소 지도이다.

Claims (7)

  1. 감염증 질환 원인균이 보유한 DNA와 특이하게 반응하는 DNA단편을 함유하는 감염증 진단용 프로브이며; 전기한 감염증 질환 원인균이 황색포도상구균(Staphylococcus aureus), 표피포도상구균(Staphylococcus epidermidis), 장내구균(Enterococcus faecalis), 녹농균(Pseudomonas aeruginosa), 대장균(Escherichia coli), 폐염간균(Klebsiella pneumonia) 및 엔터로박터 클로아케(Enterobacter cloacae)로 이루어지는 군에서 선택된 1종 이상의 세균인 특징으로 하는 감염증 진단용 프로브.
  2. 제1항에 있어서, 전기한 DNA단편이 제한효소 Hind Ⅲ으로 처리되어 조제된 절단편임을 특징으로 하는 감염증 진단용 프로브.
  3. 제1항 또는 제2항에 있어서, 전기한 감염증 진단용 프로브가 배열번호 1∼4염기배열 중 적어도 하나를 가지며, 황색포도상구균(Staphylococcus aureus)가 보유한 DNA와 특이하게 반응함을 특징으로 하는 감염증 진단용 프로브.
  4. 제1항 또는 제2항에 있어서, 전기한 감염증 진단용 프로브가 배열번호 5∼8염기배열 중 적어도 하나를 가지며, 표피포도상구균(Staphylococcus epidermidis)가 보유한 DNA와 특이하게 반응함을 특징으로 하는 감염증 진단용 프로브.
  5. 제1항 또는 제2항에 있어서, 전기한 감염증 진단용 프로브가 배열번호 9∼12염기배열 중 적어도 하나를 가지며, 장내구균(Enterococcus faecalis)이 보유한 DNA와 특이하게 반응함을 특징으로 하는 감염증 진단용 프로브.
  6. 제1항 또는 제2항에 있어서, 전기한 감염증 진단용 프로브가 배열번호 13∼16염기배열 중 적어도 하나를 가지며, 녹농균(Pseudomonas aeruginosa)이 보유한 DNA와 특이하게 반응함을 특징으로 하는 감염증 진단용 프로브.
  7. 제1항 또는 제2항에 있어서, 전기한 감염증 진단용 프로브가 배열번호 17∼23염기배열 중 적어도 하나를 가지며, 대장균(Escherichia coli), 엔터로박터 클로아케(Enterobacter cloacae) 및 폐염간균(Klebsiella pneumonia)이 보유한 DNA와 특이하게 반응함을 특징으로 하는 감염증 진단용 프로브.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950700098A 1992-07-07 1993-07-07 감염증 진단용 프로브 KR0159071B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP1992-179719 1992-07-07
JP17971992 1992-07-07
PCT/JP1993/000936 WO1994001583A1 (en) 1992-07-07 1993-07-07 Probe for diagnosing infectious disease

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KR950702640A true KR950702640A (ko) 1995-07-29
KR0159071B1 KR0159071B1 (ko) 1998-11-16

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US (5) US5807673A (ko)
EP (10) EP1498496B1 (ko)
JP (4) JP2798499B2 (ko)
KR (1) KR0159071B1 (ko)
AT (10) ATE326551T1 (ko)
AU (1) AU684250B2 (ko)
CA (1) CA2139847C (ko)
DE (10) DE69334090T2 (ko)
DK (8) DK1329518T3 (ko)
TW (1) TW256881B (ko)
WO (1) WO1994001583A1 (ko)

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DK1329518T3 (da) 2006-12-27
ATE346956T1 (de) 2006-12-15
DK1329519T3 (da) 2006-05-08
DE69334018T2 (de) 2006-11-09
ATE328118T1 (de) 2006-06-15
EP0652291B1 (en) 2003-05-28
EP0652291A4 (en) 1999-08-18
ATE241702T1 (de) 2003-06-15
TW256881B (ko) 1995-09-11
EP1329520A3 (en) 2003-12-17
DE69333958T2 (de) 2006-08-03
JP2965543B2 (ja) 1999-10-18
DK0652291T3 (da) 2003-06-23
DK1160334T3 (da) 2007-01-08
EP1498495B1 (en) 2006-05-31
EP1329520A2 (en) 2003-07-23
US5853998A (en) 1998-12-29
EP1160334A2 (en) 2001-12-05
EP1498496A2 (en) 2005-01-19
DE69333872D1 (de) 2005-10-20
EP1329518A2 (en) 2003-07-23
EP1498496B1 (en) 2006-05-17
AU4513593A (en) 1994-01-31
DK1498495T3 (da) 2006-10-09
WO1994001583A1 (en) 1994-01-20
DK1498496T3 (da) 2006-09-18
DE69334080T2 (de) 2007-05-24
EP1167544A3 (en) 2004-03-03
EP1329518A3 (en) 2003-12-10
ATE307200T1 (de) 2005-11-15
US5763188A (en) 1998-06-09
JPH10304896A (ja) 1998-11-17
DE69334028D1 (de) 2006-07-06
EP1167542A3 (en) 2003-12-17
JPH10304895A (ja) 1998-11-17
EP1167544A2 (en) 2002-01-02
DE69334060D1 (de) 2006-10-12
EP1167544B1 (en) 2005-09-14
EP1160334A3 (en) 2004-01-02
DE69334094D1 (de) 2007-01-11
CA2139847A1 (en) 1994-01-20
EP1498496A3 (en) 2005-03-09
JP3026789B2 (ja) 2000-03-27
EP1329518B1 (en) 2006-11-22
DE69333008T2 (de) 2004-02-05
EP1167542B1 (en) 2006-11-02
JP2798499B2 (ja) 1998-09-17
ATE344333T1 (de) 2006-11-15
EP1167542A2 (en) 2002-01-02
EP1498495A3 (en) 2005-03-09
EP1160334B1 (en) 2006-08-30
EP1167543A3 (en) 2004-01-07
AU684250B2 (en) 1997-12-11
KR0159071B1 (ko) 1998-11-16
EP1329519A2 (en) 2003-07-23
EP1329519B1 (en) 2005-12-28
DE69334090D1 (de) 2007-01-04
JPH10304897A (ja) 1998-11-17
DK1329520T3 (da) 2006-12-27
DE69333008D1 (de) 2003-07-03
US5770375A (en) 1998-06-23
ATE346167T1 (de) 2006-12-15
DE69334018D1 (de) 2006-06-22
DE69333958D1 (de) 2006-02-02
CA2139847C (en) 2002-05-21
ATE338141T1 (de) 2006-09-15
EP0652291A1 (en) 1995-05-10
EP1167543B1 (en) 2005-10-19
DE69334094T2 (de) 2007-05-16
DE69334080D1 (de) 2006-12-14
US5798211A (en) 1998-08-25
EP1329519A3 (en) 2004-01-07
DE69334060T2 (de) 2007-03-01
ATE326551T1 (de) 2006-06-15
EP1498495A2 (en) 2005-01-19
US5807673A (en) 1998-09-15
JP2965544B2 (ja) 1999-10-18
ATE304609T1 (de) 2005-09-15
EP1167543A2 (en) 2002-01-02
DE69334028T2 (de) 2007-05-10
DE69334090T2 (de) 2007-05-10
EP1329520B1 (en) 2006-11-29
DE69333887T2 (de) 2006-06-22
DE69333887D1 (de) 2006-03-02
DE69333872T2 (de) 2006-06-14
ATE314467T1 (de) 2006-01-15
DK1167542T3 (da) 2006-12-04

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