KR880010126A - 펙틴 라이에이즈 발현 시스템을 암호화하는 dna 서열을 함유하는 재조합 dna 분자 및 이의 제조방법 - Google Patents

펙틴 라이에이즈 발현 시스템을 암호화하는 dna 서열을 함유하는 재조합 dna 분자 및 이의 제조방법 Download PDF

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KR880010126A
KR880010126A KR1019880001000A KR880001000A KR880010126A KR 880010126 A KR880010126 A KR 880010126A KR 1019880001000 A KR1019880001000 A KR 1019880001000A KR 880001000 A KR880001000 A KR 880001000A KR 880010126 A KR880010126 A KR 880010126A
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하임 쥬타
기슬러 크리스토프
비써 자콥
코르넬리스 마리아 케스터 헤르마누스
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아놀드 세일러, 에른스트 알테르
시바-가이기 에이지
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Abstract

내용 없음

Description

펙틴 라이에이즈 발현 시스템을 암호화하는 DNA 서열을 함유하는 재조합 DNA 분자 및 이의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1도는 아스퍼질러스 니거(A. niger) 균주 N756의 Sau 3AI 단편을 함유하는 플라즈미드 PCG 3B11의 제한지도를 나타내는 개요도.
제 2도는 서열식 I DNA의 N-말단 EcoRI 단편을 함유하는 플라즈마드 PPL 29-5의 제한지도를 나타내는 개요도.

Claims (24)

  1. 펙틴 라이에이즈(PL) 발현 시스템을 암호화하는 DNA 서열을 함유하는 재조합 DNA 분자 또는 이의 유도체.
  2. 제 1항에 있어서, 프로모터, 신호서열, 및 PLI 발현시스템의 터미네이터를 함유하거나 또는 이를 함유하지 않는 구조유전자를 함유하는 재조합 DNA 분자.
  3. 제 1항에 있어서, 서열식 I의 DNA 서열을 함유하는 재조합 DNA 분자 또는 이의 유도체.
  4. 제 1항에 있어서, PCG 3B 11인 재조합 DNA 분자.
  5. 제 1항에 있어서, -1 내지 -688, 또는 -100 내지 -146번의 뉴클레오타이드 사이의 프로모터 서열, 1 내지 57번 뉴클레오타이드 사이의 신호서열, 58 내지 1366번 뉴클레오타이드중 어느 하나 사이에 뻗어 있는 터미네이터를 함유하는 재조합 DNA 분자.
  6. 제 1항에 있어서, 인트론이 존재하지 않는 PLI 구조유전자를 함유하는 재조합 DNA 분자.
  7. 제 1항에 있어서, 축퇴된(degenerated) 서열식 I의 DNA 서열을 함유하는 재조합 DNA 분자.
  8. 제 1항에 있어서, 재조합 하이브리드 벡터인 재조합 DNA 분자.
  9. 제 1항에 있어서, PLI 구조 유전자, 또는 동종 펙틴 라이에이즈 유전자 또는 이종 구조 유전자를 함유하는 벡터인 재조합 DNA 분자.
  10. 제 1항에 있어서, 하이브리드 벡터 PLHL 5인 재조합 DNA 분자.
  11. 제 1항에 있어서, 하이브리드 벡터 PLHLT 7인 재조합 DNA 분자.
  12. 펙틴 라이에이즈 발현 시스템을 암호화하는 DNA 서열을 함유하는 DNA 분자 또는 이의 유도체로 형질전환된 숙주를 배양하고, 목적하는 재조합 DNA 분자 또는 이의 유도체를 분리하거나, 이것을 시험관 내 합성으로 제조하는 단계를 포함하여 제 1항의 재조합 DNA 분자를 제조하는 방법.
  13. 제 1항의 제조합 DNA 분자를 함유하는 형질전환된 숙주.
  14. 제 13항에 있어서, 에스케리챠 콜라이(Escherichia Ccli) B 35183/PCG 3B 11(DSM 3916)인 형질전환된 숙주.
  15. 제 13항에 있어서, 제 1항의 재조합 DNA 분자 및 선별표지 플라즈미드 PCG 59 D 7로 형질전환된 아스퍼질러스 니거(Aspergillus niger) An 8(DSM 3917)인 형질전환된 숙주.
  16. 제 13항에 있어서, PLHK 3, PLHL 5 또는 PLHLT 7, 및 선별표지 플라즈미드 PCG 59 D 7으로 형질전환된 아스퍼질러스 니거 An 8(DSM 3917)인 형질전환된 숙주.
  17. 형질전환 조건하에서 임의로 선별 표지 유전자와 함께 제 1항의 재조합 DNA 분자로 숙주를 처리하고, 형질전환체를 선별하는 단계를 포함하여 제 13항의 형질전환된 숙주를 제조하는 방법.
  18. 제 17항에 있어서, 아스퍼질러스 니거 An 8이 제 1항의 재조합 DNA 분자 및 선별표지 플라즈미드 PCG 59 D 7로 공동형질 전환되는 방법.
  19. 제 18항의 하이브리드 벡터를 적합한 숙주내에서 발현시키고 폴리펩타이드를 분리함을 특징으로 하는 폴리펩타이드의 제조방법.
  20. 제 17항에 있어서, PLI를 발현시키고 분배시키기 위한 발현 하이브리드 벡터로 형질전환된 아스퍼질러스 숙주를 배양하고 영양 배지에서 PLI를 회수하는 단계를 포함하여 아스퍼질러스 중에서 PLI를 과량 생산하기 위한 방법.
  21. 선별 표지 플라즈미드 PCG 59 D 7.
  22. 제 21항의 플라즈미드를 함유하는 숙주.
  23. 제 22항에 있어서, 에스케리챠 폴라이 BJ 5183/BCG 59 D 7(DSM 3968)인 숙주.
  24. 아스퍼질러스 니거 An 8(DSM 3917).
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR88001000A 1987-02-04 1988-02-04 Recombinant dna for pectin lyase expression and its process KR970001116B1 (en)

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GB878702475A GB8702475D0 (en) 1987-02-04 1987-02-04 Expression system

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US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5447862A (en) * 1987-02-04 1995-09-05 Ciba-Geigy Corporation Pectin lyase genes of aspergillus niger
AU627443B2 (en) * 1988-01-11 1992-08-27 Xoma Corporation Novel plasmid vector with pectate lyase signal sequence
WO1989006283A1 (en) * 1988-01-11 1989-07-13 Ingene (International Genetic Engineering, Inc.) Novel plasmid vector with pectate lyase signal sequence
DE68913846T3 (de) * 1988-07-28 2002-10-02 Novartis Ag, Basel Expressionssystem.
EP0421919A3 (en) * 1989-09-02 1992-04-15 Ciba-Geigy Ag Polygalacturonase encoding fungal expression system
EP0628081A1 (en) * 1992-12-24 1994-12-14 Gist-Brocades B.V. CLONING AND EXPRESSION OF THE EXO-POLYGALACTURONASE GENE FROM $i(ASPERGILLUS)
US5858760A (en) * 1993-02-16 1999-01-12 Novo Nordisk A/S Enzyme with pectin lyase activity
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
WO1997038123A1 (en) * 1996-04-05 1997-10-16 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
US6165769A (en) * 1997-11-24 2000-12-26 Novo Nordisk A/S Pectin degrading enzymes from Bacillus licheniformis
WO1999027083A1 (en) * 1997-11-24 1999-06-03 Novo Nordisk A/S PECTIN DEGRADING ENZYMES FROM $i(BACILLUS LICHENIFORMIS)
US6187580B1 (en) 1997-11-24 2001-02-13 Novo Nordisk A/S Pectate lyases
AU4911600A (en) * 1999-06-02 2000-12-28 Novo Nordisk A/S Pectate lyase fusion for expression and secretion of polypeptides
WO2010070146A1 (en) 2008-12-19 2010-06-24 Danisco A/S Process for production of an enzyme product
EP2885407B1 (en) 2012-08-16 2018-06-13 Bangladesh Jute Research Institute Pectin degrading enzymes from macrophomina phaseolina and uses thereof
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

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US4885249A (en) 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
DE3650783T2 (de) 1985-04-15 2004-07-15 Dsm Ip Assets B.V. Verwendung des Glucoamylasepromotors aus Apergillus
DE68913846T3 (de) * 1988-07-28 2002-10-02 Novartis Ag, Basel Expressionssystem.

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NO179078B (no) 1996-04-22
FI880439A (fi) 1988-08-05
FI880439A0 (fi) 1988-02-01
IE960902L (en) 1988-08-04
DE3854947T3 (de) 2003-04-30
JP2633885B2 (ja) 1997-07-23
DD283837A5 (de) 1990-10-24
IL85293A0 (en) 1988-07-31
DD283838A5 (de) 1990-10-24
GB8702475D0 (en) 1987-03-11
PT86684B (pt) 1992-04-30
GR3018863T3 (en) 1996-05-31
PH26106A (en) 1992-02-06
IE880292L (en) 1988-08-04
ES2082746T3 (es) 1996-04-01
EP0683228A2 (en) 1995-11-22

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