DE3854947D1 - Neues Expressionssystem - Google Patents

Neues Expressionssystem

Info

Publication number
DE3854947D1
DE3854947D1 DE3854947T DE3854947T DE3854947D1 DE 3854947 D1 DE3854947 D1 DE 3854947D1 DE 3854947 T DE3854947 T DE 3854947T DE 3854947 T DE3854947 T DE 3854947T DE 3854947 D1 DE3854947 D1 DE 3854947D1
Authority
DE
Germany
Prior art keywords
hosts
preparation
recombinant dna
dna molecules
expression system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3854947T
Other languages
English (en)
Other versions
DE3854947T3 (de
DE3854947T2 (de
Inventor
Jutta Dr Heim
Christof Dr Gysler
Jacob Prof Visser
Hermanus Cornelis Maria Kester
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10611694&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3854947(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Ciba Geigy AG filed Critical Ciba Geigy AG
Publication of DE3854947D1 publication Critical patent/DE3854947D1/de
Application granted granted Critical
Publication of DE3854947T2 publication Critical patent/DE3854947T2/de
Publication of DE3854947T3 publication Critical patent/DE3854947T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/685—Aspergillus niger

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Image Generation (AREA)
  • Complex Calculations (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Hardware Redundancy (AREA)
DE3854947T 1987-02-04 1988-02-01 Neues Expressionssystem Expired - Lifetime DE3854947T3 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB878702475A GB8702475D0 (en) 1987-02-04 1987-02-04 Expression system

Publications (3)

Publication Number Publication Date
DE3854947D1 true DE3854947D1 (de) 1996-03-14
DE3854947T2 DE3854947T2 (de) 1996-11-14
DE3854947T3 DE3854947T3 (de) 2003-04-30

Family

ID=10611694

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3854947T Expired - Lifetime DE3854947T3 (de) 1987-02-04 1988-02-01 Neues Expressionssystem

Country Status (23)

Country Link
EP (2) EP0278355B2 (de)
JP (1) JP2633885B2 (de)
KR (1) KR970001116B1 (de)
AT (1) ATE133706T1 (de)
AU (1) AU625773B2 (de)
CA (1) CA1327330C (de)
DD (3) DD283837A5 (de)
DE (1) DE3854947T3 (de)
DK (1) DK175569B1 (de)
ES (1) ES2082746T5 (de)
FI (1) FI105484B (de)
GB (1) GB8702475D0 (de)
GR (1) GR3018863T3 (de)
HU (1) HU214005B (de)
IE (2) IE72506B1 (de)
IL (1) IL85293A (de)
MX (1) MX10286A (de)
NO (1) NO179078C (de)
NZ (1) NZ223392A (de)
PH (1) PH26106A (de)
PT (1) PT86684B (de)
YU (1) YU21688A (de)
ZA (1) ZA88757B (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US5447862A (en) * 1987-02-04 1995-09-05 Ciba-Geigy Corporation Pectin lyase genes of aspergillus niger
AU627443B2 (en) * 1988-01-11 1992-08-27 Xoma Corporation Novel plasmid vector with pectate lyase signal sequence
JP2980626B2 (ja) * 1988-01-11 1999-11-22 ゾーマ・コーポレーション ペクチン酸リアーゼのシグナル配列を含む新規プラスミドベクター
EP0353188B2 (de) * 1988-07-28 2002-03-06 Novartis AG Expressionssystem
EP0421919A3 (en) * 1989-09-02 1992-04-15 Ciba-Geigy Ag Polygalacturonase encoding fungal expression system
JPH07503861A (ja) * 1992-12-24 1995-04-27 ギスト　ブロカデス　ナムローゼ　フェンノートシャップ アスペルギルスからのエキソ−ポリガラクツロナーゼ遺伝子のクローニングと発現
US5858760A (en) * 1993-02-16 1999-01-12 Novo Nordisk A/S Enzyme with pectin lyase activity
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
AU2660397A (en) * 1996-04-05 1997-10-29 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
WO1999027083A1 (en) * 1997-11-24 1999-06-03 Novo Nordisk A/S PECTIN DEGRADING ENZYMES FROM $i(BACILLUS LICHENIFORMIS)
US6187580B1 (en) 1997-11-24 2001-02-13 Novo Nordisk A/S Pectate lyases
US6165769A (en) * 1997-11-24 2000-12-26 Novo Nordisk A/S Pectin degrading enzymes from Bacillus licheniformis
EP1187925B1 (de) * 1999-06-02 2005-03-30 Novozymes A/S Pektat lyase-fusion zur expression und ausscheidung von polypeptiden
WO2010070146A1 (en) 2008-12-19 2010-06-24 Danisco A/S Process for production of an enzyme product
DK2885407T3 (en) 2012-08-16 2018-07-16 Bangladesh Jute Res Institute DESTROYING ENZYMES FROM MACROPHOMINA PHASEOLINA AND ITS APPLICATIONS
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4885249A (en) 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
DE3650783T2 (de) 1985-04-15 2004-07-15 Dsm Ip Assets B.V. Verwendung des Glucoamylasepromotors aus Apergillus
EP0353188B2 (de) * 1988-07-28 2002-03-06 Novartis AG Expressionssystem

Also Published As

Publication number Publication date
DE3854947T3 (de) 2003-04-30
FI880439A (fi) 1988-08-05
NZ223392A (en) 1991-05-28
IL85293A (en) 1992-12-01
ES2082746T3 (es) 1996-04-01
DD267511A5 (de) 1989-05-03
EP0278355B1 (de) 1996-01-31
IE880292L (en) 1988-08-04
NO179078B (no) 1996-04-22
IE72506B1 (en) 1997-04-23
PT86684A (pt) 1988-03-01
KR970001116B1 (en) 1997-01-28
ZA88757B (en) 1988-08-04
DD283838A5 (de) 1990-10-24
GR3018863T3 (en) 1996-05-31
EP0278355B2 (de) 2002-10-23
FI105484B (fi) 2000-08-31
NO179078C (no) 1996-07-31
YU21688A (en) 1991-04-30
DK54588A (da) 1988-08-05
ATE133706T1 (de) 1996-02-15
JPS63273480A (ja) 1988-11-10
IE960902L (en) 1988-08-04
JP2633885B2 (ja) 1997-07-23
ES2082746T5 (es) 2003-06-16
EP0683228A2 (de) 1995-11-22
PT86684B (pt) 1992-04-30
HUT46357A (en) 1988-10-28
DE3854947T2 (de) 1996-11-14
EP0278355A3 (en) 1989-12-20
GB8702475D0 (en) 1987-03-11
IL85293A0 (en) 1988-07-31
PH26106A (en) 1992-02-06
NO880470L (no) 1988-08-05
CA1327330C (en) 1994-03-01
FI880439A0 (fi) 1988-02-01
HU214005B (hu) 1998-08-28
NO880470D0 (no) 1988-02-03
MX10286A (es) 1993-12-01
EP0683228A3 (de) 1996-03-06
DK175569B1 (da) 2004-12-13
AU1123488A (en) 1988-08-18
KR880010126A (ko) 1988-10-07
DD283837A5 (de) 1990-10-24
EP0278355A2 (de) 1988-08-17
DK54588D0 (da) 1988-02-03
AU625773B2 (en) 1992-07-16

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Legal Events

Date Code Title Description
8363 Opposition against the patent
8327 Change in the person/name/address of the patent owner

Owner name: NOVARTIS AG, BASEL, CH

8366 Restricted maintained after opposition proceedings
8328 Change in the person/name/address of the agent

Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN