KR20230004466A - 황산화된 다당류의 제조 방법 및 paps의 제조 방법 - Google Patents

황산화된 다당류의 제조 방법 및 paps의 제조 방법 Download PDF

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KR20230004466A
KR20230004466A KR1020227034289A KR20227034289A KR20230004466A KR 20230004466 A KR20230004466 A KR 20230004466A KR 1020227034289 A KR1020227034289 A KR 1020227034289A KR 20227034289 A KR20227034289 A KR 20227034289A KR 20230004466 A KR20230004466 A KR 20230004466A
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transformant
gene
producing
gene encoding
extract
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KR1020227034289A
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겐타 후쿠나가
아키 니시바라
료스케 가토
마사히로 구라츠
미키로 하야시
신-이치 하시모토
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렌슬러 폴리테크닉 인스티튜트
가부시키가이샤 오츠까 세이야꾸 고죠
기린 바이오머터리얼즈 컴퍼니 리미티드
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Publication of KR20230004466A publication Critical patent/KR20230004466A/ko

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    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
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    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
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    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
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    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
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    • C12Y501/03Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03017Heparosan-N-sulfate-glucuronate 5-epimerase (5.1.3.17)
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    • C12R2001/15Corynebacterium

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  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
KR1020227034289A 2020-04-03 2021-04-02 황산화된 다당류의 제조 방법 및 paps의 제조 방법 KR20230004466A (ko)

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Application Number Priority Date Filing Date Title
JPJP-P-PCT/JP2020/015388 2020-04-03
PCT/JP2020/015388 WO2021199445A1 (en) 2020-04-03 2020-04-03 Method for producing sulfated polysaccharide and method for producing paps
PCT/JP2021/014337 WO2021201282A1 (en) 2020-04-03 2021-04-02 Method for producing sulfated polysaccharide and method for producing paps

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KR20230004466A true KR20230004466A (ko) 2023-01-06

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US (1) US20230159969A1 (ja)
EP (1) EP4127204A4 (ja)
JP (2) JP7398684B2 (ja)
KR (1) KR20230004466A (ja)
CN (1) CN115605603A (ja)
AU (1) AU2021249958B2 (ja)
BR (1) BR112022019828A2 (ja)
CA (1) CA3174029A1 (ja)
IL (1) IL296974A (ja)
SA (1) SA522440743B1 (ja)
TW (1) TW202204632A (ja)
WO (2) WO2021199445A1 (ja)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113046403B (zh) * 2020-09-30 2023-04-28 江南大学 一种基于构建atp再生系统高效催化合成paps的方法
CN113046402B (zh) * 2020-09-30 2023-04-28 江南大学 一种基于构建双功能酶合成paps的方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5830464B2 (ja) 1980-04-07 1983-06-29 株式会社 河原 作業台
JP4505011B2 (ja) 2005-01-25 2010-07-14 ヤマサ醤油株式会社 3’−ホスホアデノシン−5’−ホスホ硫酸の酵素合成法
WO2012057336A1 (ja) 2010-10-29 2012-05-03 ヤマサ醤油株式会社 育毛剤組成物
JP2012201665A (ja) 2011-03-28 2012-10-22 Yamasa Shoyu Co Ltd 保湿作用を有する皮膚外用剤
US8771995B2 (en) 2005-05-12 2014-07-08 The University Of North Carolina At Chapel Hill Enzymatic synthesis of sulfated polysaccharides
WO2018048973A1 (en) 2016-09-07 2018-03-15 Rensselaer Polytechnic Institute Biosynthetic heparin
WO2020013346A1 (en) 2018-07-11 2020-01-16 Ajinomoto Co., Inc. Method for enzymatic sulfurylation of alcohols and amines using bacterium of the family enterobacteriaceae

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5919673A (en) * 1995-03-22 1999-07-06 The Scripps Research Institute One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
JP2002530087A (ja) * 1998-11-18 2002-09-17 ネオーズ テクノロジーズ, インコーポレイテッド オリゴ糖の安価な産生
KR20110035805A (ko) * 2009-09-30 2011-04-06 씨제이제일제당 (주) 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법
KR101203856B1 (ko) * 2011-08-24 2012-11-21 씨제이제일제당 (주) 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산
RU2017104525A (ru) * 2014-07-14 2018-08-14 Басф Се Биотехнологическое получение lnt, lnnt и их фукозилированных производных
CN116287017A (zh) * 2016-02-24 2023-06-23 化学生物有限公司 用于生产芳基硫酸酯的改进的生物方法
CN117417912A (zh) * 2017-09-05 2024-01-19 味之素株式会社 2-o-硫酸化酶突变体和3-o-硫酸化酶突变体及其使用方法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5830464B2 (ja) 1980-04-07 1983-06-29 株式会社 河原 作業台
JP4505011B2 (ja) 2005-01-25 2010-07-14 ヤマサ醤油株式会社 3’−ホスホアデノシン−5’−ホスホ硫酸の酵素合成法
US8771995B2 (en) 2005-05-12 2014-07-08 The University Of North Carolina At Chapel Hill Enzymatic synthesis of sulfated polysaccharides
WO2012057336A1 (ja) 2010-10-29 2012-05-03 ヤマサ醤油株式会社 育毛剤組成物
JP2012201665A (ja) 2011-03-28 2012-10-22 Yamasa Shoyu Co Ltd 保湿作用を有する皮膚外用剤
WO2018048973A1 (en) 2016-09-07 2018-03-15 Rensselaer Polytechnic Institute Biosynthetic heparin
WO2020013346A1 (en) 2018-07-11 2020-01-16 Ajinomoto Co., Inc. Method for enzymatic sulfurylation of alcohols and amines using bacterium of the family enterobacteriaceae

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
NPL 1: Glycobiology vol. 21, no. 6, pp. 771-780, 2011
NPL 2: Biosci. Biotechnol. Biochem. 65 (3), 644-650, 2001
NPL 3: Carbohydrate Polymers 122 (2015) 399-407
NPL 4: Advanced Drug Delivery Reviews 97 (2016) 237-249
NPL 5: Metabolic Engineering 27 (2015) 92-100
NPL 6: Biotechnology and Bioengineering 115 (2018) 1561-1570
PTL 3: JP-A-H5-137588

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US20230159969A1 (en) 2023-05-25
EP4127204A4 (en) 2024-06-26
AU2021249958B2 (en) 2024-08-29
WO2021199445A1 (en) 2021-10-07
JP2023515250A (ja) 2023-04-12
BR112022019828A2 (pt) 2023-02-23
JP2023171870A (ja) 2023-12-05
CN115605603A (zh) 2023-01-13
TW202204632A (zh) 2022-02-01
EP4127204A1 (en) 2023-02-08
IL296974A (en) 2022-12-01
CA3174029A1 (en) 2021-10-07
SA522440743B1 (ar) 2024-05-30
AU2021249958A1 (en) 2022-09-22
WO2021201282A1 (en) 2021-10-07
JP7398684B2 (ja) 2023-12-15

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