BR112022019828A2 - Método para a produção de polissacarídeo sulfatado e método para a produção de paps - Google Patents

Método para a produção de polissacarídeo sulfatado e método para a produção de paps

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Publication number
BR112022019828A2
BR112022019828A2 BR112022019828A BR112022019828A BR112022019828A2 BR 112022019828 A2 BR112022019828 A2 BR 112022019828A2 BR 112022019828 A BR112022019828 A BR 112022019828A BR 112022019828 A BR112022019828 A BR 112022019828A BR 112022019828 A2 BR112022019828 A2 BR 112022019828A2
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BR
Brazil
Prior art keywords
paps
production
producing
transformant
treated
Prior art date
Application number
BR112022019828A
Other languages
English (en)
Inventor
Fukunaga Kenta
Nishibara Aki
Kato Ryosuke
Kuratsu Masahiro
Hayashi Mikiro
Hashimoto Shin-Ichi
Original Assignee
Rensselaer Polytech Inst
Otsuka Pharma Factory Inc
Kirin Biomaterials Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Rensselaer Polytech Inst, Otsuka Pharma Factory Inc, Kirin Biomaterials Co Ltd filed Critical Rensselaer Polytech Inst
Publication of BR112022019828A2 publication Critical patent/BR112022019828A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01025—Adenylyl-sulfate kinase (2.7.1.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07004—Sulfate adenylyltransferase (2.7.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/02—Sulfotransferases (2.8.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03017—Heparosan-N-sulfate-glucuronate 5-epimerase (5.1.3.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

MÉTODO PARA A PRODUÇÃO DE POLISSACARÍDEO SULFATADO E MÉTODO PARA A PRODUÇÃO DE PAPS. A presente invenção refere-se a um método para facilmente produzir polissacarídeos sulfatados pela reação de um sistema de produção/regeneração de PAPS utilizando a atividade metabólica de um microrganismo ou uma matéria tratada do mesmo com um microrganismo que expressa uma enzima de sulfatação ou uma matéria tratada ou extrato da mesma, após a mistura de matérias-primas baratas, tal como sulfato de magnésio. Outro objetivo da presente invenção é fornecer um método prático para produzir PAPS a partir de matérias-primas baratas. A presente invenção refere-se a um método para a produção de um polissacarídeo sulfatado e um método para a produção de PAPS, os métodos compreendendo uma etapa de preparação de um transformante (a) de uma bactéria do gênero Corynebacterium, que compreende pelo menos um gene que codifica uma ATP sulfurilase e um gene que codifica uma APS cinase que são introduzidos no mesmo de uma maneira expressável, e em que uma membrana plasmática celular do transformante (a) é permeável à substância, ou uma matéria tratada do transformante (a); e uma etapa de condução de uma reação para a produção de PAPS usando uma solução de reação contendo ATP ou uma fonte de ATP, uma fonte de íon de sulfato e o transformante (a) ou a matéria tratada do mesmo.
BR112022019828A 2020-04-03 2021-04-02 Método para a produção de polissacarídeo sulfatado e método para a produção de paps BR112022019828A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/JP2020/015388 WO2021199445A1 (en) 2020-04-03 2020-04-03 Method for producing sulfated polysaccharide and method for producing paps
PCT/JP2021/014337 WO2021201282A1 (en) 2020-04-03 2021-04-02 Method for producing sulfated polysaccharide and method for producing paps

Publications (1)

Publication Number Publication Date
BR112022019828A2 true BR112022019828A2 (pt) 2023-02-23

Family

ID=77928740

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022019828A BR112022019828A2 (pt) 2020-04-03 2021-04-02 Método para a produção de polissacarídeo sulfatado e método para a produção de paps

Country Status (11)

Country Link
US (1) US20230159969A1 (pt)
EP (1) EP4127204A4 (pt)
JP (2) JP7398684B2 (pt)
KR (1) KR20230004466A (pt)
CN (1) CN115605603A (pt)
AU (1) AU2021249958A1 (pt)
BR (1) BR112022019828A2 (pt)
CA (1) CA3174029A1 (pt)
IL (1) IL296974A (pt)
TW (1) TW202204632A (pt)
WO (2) WO2021199445A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113046403B (zh) * 2020-09-30 2023-04-28 江南大学 一种基于构建atp再生系统高效催化合成paps的方法
CN113046402B (zh) * 2020-09-30 2023-04-28 江南大学 一种基于构建双功能酶合成paps的方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5830464B2 (ja) 1980-04-07 1983-06-29 株式会社 河原 作業台
US5919673A (en) * 1995-03-22 1999-07-06 The Scripps Research Institute One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
MXPA01004982A (es) * 1998-11-18 2004-09-27 Neose Technologies Inc Manufactura de bajo costo de oligosacaridos.
EP2100956B1 (en) * 2005-01-25 2018-11-21 Yamasa Corporation Method of enzymatically synthesizing 3'-phosphoadenosine-5'-phophosulfate
WO2006124801A2 (en) * 2005-05-12 2006-11-23 The University Of North Carolina At Chapel Hill Enzymatic synthesis of sulfated polysaccharides
KR20110035805A (ko) * 2009-09-30 2011-04-06 씨제이제일제당 (주) 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법
WO2012057336A1 (ja) 2010-10-29 2012-05-03 ヤマサ醤油株式会社 育毛剤組成物
JP5455248B2 (ja) 2011-03-28 2014-03-26 ヤマサ醤油株式会社 保湿作用を有する皮膚外用剤
KR101203856B1 (ko) * 2011-08-24 2012-11-21 씨제이제일제당 (주) 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산
US20170204443A1 (en) * 2014-07-14 2017-07-20 Basf Se Biotechnological production of lnt, lnnt and the fucosylated derivatives thereof
US10954536B2 (en) * 2016-02-24 2021-03-23 Cysbio Aps Modified Escherichia coli having an increased expression of a CysP transporter, an ATP sulfurylase, an APS kinase and a PAP phosphatase
TWI769176B (zh) * 2016-09-07 2022-07-01 瑞瑟勒綜合技術協會 生合成肝素
WO2019050051A1 (en) * 2017-09-05 2019-03-14 Ajinomoto Co., Inc. MUTANT OF 2-O-SULFATION ENZYME AND MUTANT OF 3-O-SULFATION ENZYME, AND METHOD OF USE
JP7424320B2 (ja) 2018-07-11 2024-01-30 味の素株式会社 腸内細菌科の細菌を用いてアルコール類およびアミン類を酵素によりスルフリル化する方法

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Publication number Publication date
US20230159969A1 (en) 2023-05-25
CA3174029A1 (en) 2021-10-07
KR20230004466A (ko) 2023-01-06
IL296974A (en) 2022-12-01
AU2021249958A1 (en) 2022-09-22
WO2021199445A1 (en) 2021-10-07
WO2021201282A1 (en) 2021-10-07
CN115605603A (zh) 2023-01-13
EP4127204A1 (en) 2023-02-08
JP7398684B2 (ja) 2023-12-15
TW202204632A (zh) 2022-02-01
JP2023171870A (ja) 2023-12-05
JP2023515250A (ja) 2023-04-12
EP4127204A4 (en) 2024-06-26

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