BR112018000168A2 - método para produção de células ips de alta qualidade - Google Patents
método para produção de células ips de alta qualidadeInfo
- Publication number
- BR112018000168A2 BR112018000168A2 BR112018000168-6A BR112018000168A BR112018000168A2 BR 112018000168 A2 BR112018000168 A2 BR 112018000168A2 BR 112018000168 A BR112018000168 A BR 112018000168A BR 112018000168 A2 BR112018000168 A2 BR 112018000168A2
- Authority
- BR
- Brazil
- Prior art keywords
- ips cells
- manufacturing
- cells
- ips cell
- gene
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- 210000004027 cell Anatomy 0.000 abstract 6
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 230000008672 reprogramming Effects 0.000 abstract 2
- 210000001082 somatic cell Anatomy 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 239000003623 enhancer Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/604—Klf-4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/605—Nanog
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Transplantation (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
a presente invenção refere-se a um aperfeiçoador de qualidade de célula ips, um método para produção de células ips, células ips produzidas usando o dito método de produção e uma composição para produção de células ips. o presente método para produção de células ips é caracterizado por conter uma etapa em que (a) substância de reprogramação nuclear e (b) gene h1foo ou produto de gene do mesmo são introduzidos em células somáticas. através da introdução não apenas de uma substância de reprogramação nuclear, mas também um gene h1foo ou um produto de gene do mesmo em células somáticas, é possível produzir células ips de alta qualidade em maior quantidade.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015-138645 | 2015-07-10 | ||
| JP2015138645 | 2015-07-10 | ||
| PCT/JP2016/003282 WO2017010080A1 (ja) | 2015-07-10 | 2016-07-11 | 高品質なｉＰＳ細胞の製造方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112018000168A2 true BR112018000168A2 (pt) | 2018-09-04 |
Family
ID=57757358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112018000168-6A BR112018000168A2 (pt) | 2015-07-10 | 2016-07-11 | método para produção de células ips de alta qualidade |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US10544396B2 (pt) |
| EP (1) | EP3333256B1 (pt) |
| JP (1) | JP6927578B2 (pt) |
| KR (1) | KR20180029976A (pt) |
| CN (1) | CN107709553A (pt) |
| AU (1) | AU2016293647B2 (pt) |
| BR (1) | BR112018000168A2 (pt) |
| CA (1) | CA2990476C (pt) |
| HK (1) | HK1247640A1 (pt) |
| IL (1) | IL256384B2 (pt) |
| RU (1) | RU2721266C1 (pt) |
| SG (1) | SG11201710619RA (pt) |
| WO (1) | WO2017010080A1 (pt) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200017837A1 (en) * | 2017-02-03 | 2020-01-16 | National University Corporation Kobe University | Method For Producing Induced Pluripotent Stem Cells |
| EP4032512A1 (en) | 2018-05-03 | 2022-07-27 | Gals Bio Ltd. | Intravaginal device |
| WO2020179071A1 (ja) * | 2019-03-07 | 2020-09-10 | オリンパス株式会社 | ｉＰＳ細胞の選定方法、及びｉＰＳ細胞の作製方法 |
| CN116018402A (zh) | 2020-09-04 | 2023-04-25 | 心脏康复株式会社 | iPS细胞的品质改善剂、iPS细胞的制备方法、iPS细胞、及iPS细胞制备用组合物 |
| CN113563428B (zh) * | 2021-06-22 | 2022-08-02 | 呈诺再生医学科技(珠海横琴新区)有限公司 | 特定空间结构多肽及其在制备iPSC中的应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4688793B2 (ja) | 2004-03-23 | 2011-05-25 | 敏宏 赤池 | 多能性幹細胞の増殖方法 |
| CN101356270B (zh) | 2005-12-13 | 2014-02-12 | 国立大学法人京都大学 | 核重新编程因子 |
| AU2008297024B2 (en) * | 2007-10-31 | 2014-08-28 | Kyoto University | Nuclear reprogramming method |
| WO2009114949A1 (en) * | 2008-03-20 | 2009-09-24 | UNIVERSITé LAVAL | Methods for deprogramming somatic cells and uses thereof |
| CN101835890B (zh) | 2008-06-27 | 2013-07-24 | 国立大学法人京都大学 | 有效建立诱导的多能干细胞的方法 |
| JP2011004674A (ja) | 2009-06-26 | 2011-01-13 | Fujitsu Ltd | 誘導多能性幹細胞（ｉＰＳ細胞）の製造方法 |
| CA2788635A1 (en) * | 2010-02-01 | 2011-08-04 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced efficiency of induced pluripotent stem cell generation |
| RU2458983C1 (ru) | 2011-07-18 | 2012-08-20 | Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации | Способ получения индуцированных плюрипотентных стволовых клеток из фибробластов пациентов с болезнью хангингтона |
| JP2014217344A (ja) | 2013-05-10 | 2014-11-20 | 学校法人 埼玉医科大学 | 人工多能性幹細胞製造用組成物、及び人工多能性幹細胞の製造方法 |
| JP2014217345A (ja) | 2013-05-10 | 2014-11-20 | 学校法人 埼玉医科大学 | 人工多能性幹細胞製造用組成物、及び人工多能性幹細胞の製造方法 |
-
2016
- 2016-07-11 JP JP2017528287A patent/JP6927578B2/ja active Active
- 2016-07-11 KR KR1020177037053A patent/KR20180029976A/ko not_active Application Discontinuation
- 2016-07-11 US US15/737,988 patent/US10544396B2/en active Active
- 2016-07-11 AU AU2016293647A patent/AU2016293647B2/en active Active
- 2016-07-11 EP EP16824065.3A patent/EP3333256B1/en active Active
- 2016-07-11 RU RU2018100118A patent/RU2721266C1/ru active
- 2016-07-11 IL IL256384A patent/IL256384B2/en unknown
- 2016-07-11 WO PCT/JP2016/003282 patent/WO2017010080A1/ja active Application Filing
- 2016-07-11 CN CN201680037461.3A patent/CN107709553A/zh active Pending
- 2016-07-11 BR BR112018000168-6A patent/BR112018000168A2/pt active Search and Examination
- 2016-07-11 CA CA2990476A patent/CA2990476C/en active Active
- 2016-07-11 SG SG11201710619RA patent/SG11201710619RA/en unknown
-
2018
- 2018-05-30 HK HK18107080.6A patent/HK1247640A1/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPWO2017010080A1 (ja) | 2018-05-24 |
| IL256384A (en) | 2018-02-28 |
| EP3333256A1 (en) | 2018-06-13 |
| EP3333256B1 (en) | 2023-10-25 |
| EP3333256A4 (en) | 2019-01-16 |
| AU2016293647A1 (en) | 2018-01-18 |
| IL256384B1 (en) | 2023-08-01 |
| IL256384B2 (en) | 2023-12-01 |
| HK1247640A1 (zh) | 2018-09-28 |
| AU2016293647B2 (en) | 2019-07-11 |
| CN107709553A (zh) | 2018-02-16 |
| JP6927578B2 (ja) | 2021-09-01 |
| WO2017010080A1 (ja) | 2017-01-19 |
| CA2990476A1 (en) | 2017-01-19 |
| US20180298346A1 (en) | 2018-10-18 |
| US10544396B2 (en) | 2020-01-28 |
| RU2721266C1 (ru) | 2020-05-18 |
| SG11201710619RA (en) | 2018-01-30 |
| CA2990476C (en) | 2021-09-07 |
| KR20180029976A (ko) | 2018-03-21 |
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Legal Events
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| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] |
Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |
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| B07G | Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette] | ||
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] |