KR20200136463A - Gmp-수준 무혈청 현탁 세포 사용에 의한 렌티바이러스의 대규모 제조 방법 - Google Patents

Gmp-수준 무혈청 현탁 세포 사용에 의한 렌티바이러스의 대규모 제조 방법 Download PDF

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KR20200136463A
KR20200136463A KR1020207030733A KR20207030733A KR20200136463A KR 20200136463 A KR20200136463 A KR 20200136463A KR 1020207030733 A KR1020207030733 A KR 1020207030733A KR 20207030733 A KR20207030733 A KR 20207030733A KR 20200136463 A KR20200136463 A KR 20200136463A
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cells
liquid exchange
transfection
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이 홍
팅 얀
지앙구오 잉
하오지에 장
루이 장
리 장
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셀룰라 바이오메디신 그룹 에이치케이 리미티드
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material

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  • Life Sciences & Earth Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020207030733A 2018-03-29 2019-03-28 Gmp-수준 무혈청 현탁 세포 사용에 의한 렌티바이러스의 대규모 제조 방법 Ceased KR20200136463A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201810273392.7 2018-03-29
CN201810273392.7A CN110317791A (zh) 2018-03-29 2018-03-29 Gmp级无血清悬浮细胞大规模生产慢病毒的方法
PCT/CN2019/080215 WO2019184996A1 (zh) 2018-03-29 2019-03-28 Gmp级无血清悬浮细胞大规模生产慢病毒的方法

Publications (1)

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KR20200136463A true KR20200136463A (ko) 2020-12-07

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KR1020207030733A Ceased KR20200136463A (ko) 2018-03-29 2019-03-28 Gmp-수준 무혈청 현탁 세포 사용에 의한 렌티바이러스의 대규모 제조 방법

Country Status (8)

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US (3) US11845962B2 (https=)
EP (1) EP3778880A4 (https=)
JP (2) JP2021519108A (https=)
KR (1) KR20200136463A (https=)
CN (1) CN110317791A (https=)
AU (1) AU2019241301B2 (https=)
SG (1) SG11202009826PA (https=)
WO (1) WO2019184996A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110317791A (zh) * 2018-03-29 2019-10-11 西比曼生物科技（香港）有限公司 Gmp级无血清悬浮细胞大规模生产慢病毒的方法
CN110894494B (zh) * 2019-11-22 2022-09-27 广西梧州制药（集团）股份有限公司 一种大规模高密度悬浮培养293细胞高产腺病毒的方法
CN113832112A (zh) * 2020-06-24 2021-12-24 重庆精准生物技术有限公司 一种制备慢病毒的混合方法及混合系统
EP4330381A1 (en) * 2021-04-27 2024-03-06 Novartis AG Viral vector production system
CN113265425A (zh) * 2021-05-08 2021-08-17 苏州博腾生物制药有限公司 基于悬浮细胞的car-cd19慢病毒制备工艺
CN114990077A (zh) * 2022-08-01 2022-09-02 深圳市先康达生命科学有限公司 一种慢病毒包装与纯化方法

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AU2002348151A1 (en) 2001-11-05 2003-05-19 Genvec, Inc. Viral vector production methods and compositions
US20030175688A1 (en) 2002-03-15 2003-09-18 Rukmini Pennathur-Das Method for the purification and production of oncolytic adenoviruses
WO2007095336A2 (en) 2006-02-15 2007-08-23 The Immune Response Corporation Production of hiv
PL2007883T3 (pl) 2006-04-20 2012-07-31 Wyeth Llc Sposób oczyszczania do izolacji oczyszczonego wirusa pęcherzykowatego zapalenia jamy ustnej z hodowli komórkowej
JP2011507523A (ja) 2007-12-21 2011-03-10 ワイス・エルエルシー 遺伝子改変した弱毒化水疱性口内炎ウイルス、組成物およびその使用方法
CN102203242B (zh) 2008-11-03 2013-06-12 克鲁塞尔荷兰公司 产生腺病毒载体的方法
PT2782997T (pt) 2011-11-24 2018-04-09 Genethon Sistema de produção de vetor lentiviral escalável compatível com aplicações farmacêuticas industriais
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
CN103881984A (zh) * 2013-12-06 2014-06-25 深圳市赛百诺基因技术有限公司 无血清悬浮细胞生产重组腺病毒的方法及其药品制剂生产方法
FR3014901B1 (fr) 2013-12-17 2017-06-09 Genethon Procede de purification de virus ou vecteurs viraux enveloppes
CN104371982A (zh) 2014-10-21 2015-02-25 武汉维诺赛生物技术有限公司 一种慢病毒的纯化方法
EP3054007A1 (en) 2015-02-09 2016-08-10 Institut National De La Sante Et De La Recherche Medicale (Inserm) Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step
CN107043784A (zh) * 2016-02-05 2017-08-15 上海吉凯基因化学技术有限公司 一种慢病毒载体的制备方法
CN107523555A (zh) * 2016-06-22 2017-12-29 武汉光谷人福生物医药有限公司 获得病毒的方法
CN107841482B (zh) * 2016-09-21 2021-07-27 上海倍谙基生物科技有限公司 Mdck细胞无血清悬浮培养技术生产h9亚型流感疫苗
CN106434571A (zh) * 2016-10-19 2017-02-22 深圳源兴基因技术有限公司 一种wave无血清培养悬浮细胞制备重组腺病毒的方法
CN106474466B (zh) 2016-12-07 2018-04-13 申联生物医药（上海）股份有限公司 一种口蹄疫疫苗的制备方法
CN107384877B (zh) 2017-08-18 2020-09-08 深圳源兴基因技术有限公司 一种慢病毒的纯化方法
CN107630037A (zh) 2017-10-19 2018-01-26 和元生物技术（上海）股份有限公司 一种获得高纯度腺相关病毒载体的纯化工艺
CN110317832B (zh) 2018-03-28 2022-07-05 西比曼生物科技（香港）有限公司 Gmp级规模化制备重组慢病毒载体的纯化制剂的方法
CN110317791A (zh) * 2018-03-29 2019-10-11 西比曼生物科技（香港）有限公司 Gmp级无血清悬浮细胞大规模生产慢病毒的方法

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US20250084386A1 (en) 2025-03-13
AU2019241301B2 (en) 2025-04-17
EP3778880A4 (en) 2021-12-29
JP2021519108A (ja) 2021-08-10
AU2019241301A1 (en) 2020-10-22
WO2019184996A1 (zh) 2019-10-03
US20240060053A1 (en) 2024-02-22
US12180513B2 (en) 2024-12-31
US11845962B2 (en) 2023-12-19
EP3778880A1 (en) 2021-02-17
SG11202009826PA (en) 2020-11-27
CN110317791A (zh) 2019-10-11
US20210363497A1 (en) 2021-11-25
JP2024075766A (ja) 2024-06-04

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