CN110317791A - Gmp级无血清悬浮细胞大规模生产慢病毒的方法 - Google Patents

Gmp级无血清悬浮细胞大规模生产慢病毒的方法 Download PDF

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Publication number
CN110317791A
CN110317791A CN201810273392.7A CN201810273392A CN110317791A CN 110317791 A CN110317791 A CN 110317791A CN 201810273392 A CN201810273392 A CN 201810273392A CN 110317791 A CN110317791 A CN 110317791A
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culture
transfection
cells
time
medium
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Chinese (zh)
Inventor
洪谊
闫听
应降果
张豪杰
张露亿
张丽
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Shanghai Abelzeta Ltd
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Cellular Biomedicine Group HK Ltd
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Priority to CN201810273392.7A priority Critical patent/CN110317791A/zh
Priority to EP19775992.1A priority patent/EP3778880A4/en
Priority to KR1020207030733A priority patent/KR20200136463A/ko
Priority to PCT/CN2019/080215 priority patent/WO2019184996A1/zh
Priority to AU2019241301A priority patent/AU2019241301B2/en
Priority to JP2021501071A priority patent/JP2021519108A/ja
Priority to US17/041,578 priority patent/US11845962B2/en
Priority to SG11202009826PA priority patent/SG11202009826PA/en
Publication of CN110317791A publication Critical patent/CN110317791A/zh
Priority to US18/498,879 priority patent/US12180513B2/en
Priority to JP2024051544A priority patent/JP2024075766A/ja
Priority to US18/962,655 priority patent/US20250084386A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material

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  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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CN201810273392.7A 2018-03-29 2018-03-29 Gmp级无血清悬浮细胞大规模生产慢病毒的方法 Pending CN110317791A (zh)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CN201810273392.7A CN110317791A (zh) 2018-03-29 2018-03-29 Gmp级无血清悬浮细胞大规模生产慢病毒的方法
JP2021501071A JP2021519108A (ja) 2018-03-29 2019-03-28 Ｇｍｐレベルの無血清懸濁液細胞を用いるレンチウイルスの大規模製造方法
KR1020207030733A KR20200136463A (ko) 2018-03-29 2019-03-28 Gmp-수준 무혈청 현탁 세포 사용에 의한 렌티바이러스의 대규모 제조 방법
PCT/CN2019/080215 WO2019184996A1 (zh) 2018-03-29 2019-03-28 Gmp级无血清悬浮细胞大规模生产慢病毒的方法
AU2019241301A AU2019241301B2 (en) 2018-03-29 2019-03-28 Method for large-scale production of lentivirus by using GMP-level serum-free suspension cells
EP19775992.1A EP3778880A4 (en) 2018-03-29 2019-03-28 Method for large-scale production of lentivirus by using gmp-level serum-free suspension cells
US17/041,578 US11845962B2 (en) 2018-03-29 2019-03-28 Method for large-scale production of lentivirus by using GMP-level serum-free suspension cells
SG11202009826PA SG11202009826PA (en) 2018-03-29 2019-03-28 Method for large-scale production of lentivirus by using gmp-level serum-free suspension cells
US18/498,879 US12180513B2 (en) 2018-03-29 2023-10-31 Method for large-scale production of lentivirus by using GMP-level serum-free suspension cells
JP2024051544A JP2024075766A (ja) 2018-03-29 2024-03-27 Ｇｍｐレベルの無血清懸濁液細胞を用いるレンチウイルスの大規模製造方法
US18/962,655 US20250084386A1 (en) 2018-03-29 2024-11-27 Method for large-scale production of lentivirus by using gmp-level serum-free suspension cells

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CN201810273392.7A CN110317791A (zh) 2018-03-29 2018-03-29 Gmp级无血清悬浮细胞大规模生产慢病毒的方法

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Country Status (8)

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US (3) US11845962B2 (https=)
EP (1) EP3778880A4 (https=)
JP (2) JP2021519108A (https=)
KR (1) KR20200136463A (https=)
CN (1) CN110317791A (https=)
AU (1) AU2019241301B2 (https=)
SG (1) SG11202009826PA (https=)
WO (1) WO2019184996A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110894494A (zh) * 2019-11-22 2020-03-20 广西梧州制药（集团）股份有限公司 一种大规模高密度悬浮培养293细胞高产腺病毒的方法
CN113265425A (zh) * 2021-05-08 2021-08-17 苏州博腾生物制药有限公司 基于悬浮细胞的car-cd19慢病毒制备工艺

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110317791A (zh) * 2018-03-29 2019-10-11 西比曼生物科技（香港）有限公司 Gmp级无血清悬浮细胞大规模生产慢病毒的方法
CN113832112A (zh) * 2020-06-24 2021-12-24 重庆精准生物技术有限公司 一种制备慢病毒的混合方法及混合系统
EP4330381A1 (en) * 2021-04-27 2024-03-06 Novartis AG Viral vector production system
CN114990077A (zh) * 2022-08-01 2022-09-02 深圳市先康达生命科学有限公司 一种慢病毒包装与纯化方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110894494A (zh) * 2019-11-22 2020-03-20 广西梧州制药（集团）股份有限公司 一种大规模高密度悬浮培养293细胞高产腺病毒的方法
CN113265425A (zh) * 2021-05-08 2021-08-17 苏州博腾生物制药有限公司 基于悬浮细胞的car-cd19慢病毒制备工艺

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US20250084386A1 (en) 2025-03-13
AU2019241301B2 (en) 2025-04-17
EP3778880A4 (en) 2021-12-29
JP2021519108A (ja) 2021-08-10
AU2019241301A1 (en) 2020-10-22
KR20200136463A (ko) 2020-12-07
WO2019184996A1 (zh) 2019-10-03
US20240060053A1 (en) 2024-02-22
US12180513B2 (en) 2024-12-31
US11845962B2 (en) 2023-12-19
EP3778880A1 (en) 2021-02-17
SG11202009826PA (en) 2020-11-27
US20210363497A1 (en) 2021-11-25
JP2024075766A (ja) 2024-06-04

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