JP2021519108A - Ｇｍｐレベルの無血清懸濁液細胞を用いるレンチウイルスの大規模製造方法 - Google Patents

Ｇｍｐレベルの無血清懸濁液細胞を用いるレンチウイルスの大規模製造方法 Download PDF

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JP2021519108A
JP2021519108A JP2021501071A JP2021501071A JP2021519108A JP 2021519108 A JP2021519108 A JP 2021519108A JP 2021501071 A JP2021501071 A JP 2021501071A JP 2021501071 A JP2021501071 A JP 2021501071A JP 2021519108 A JP2021519108 A JP 2021519108A
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culture
cell
transfection
cells
liquid exchange
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JPWO2019184996A5 (https=
JP2021519108A5 (https=
Inventor
ホン，イー
ヤン，ティン
イン，ジャングオ
ジャン，ハオジエ
ジャン，ルイ
ジャン，リー
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Cellular Biomedicine Group HK Ltd
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Cellular Biomedicine Group HK Ltd
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Publication of JP2021519108A5 publication Critical patent/JP2021519108A5/ja
Priority to JP2024051544A priority Critical patent/JP2024075766A/ja
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material

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  • Life Sciences & Earth Sciences (AREA)
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JP2021501071A 2018-03-29 2019-03-28 Ｇｍｐレベルの無血清懸濁液細胞を用いるレンチウイルスの大規模製造方法 Pending JP2021519108A (ja)

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JP2024051544A JP2024075766A (ja) 2018-03-29 2024-03-27 Ｇｍｐレベルの無血清懸濁液細胞を用いるレンチウイルスの大規模製造方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201810273392.7 2018-03-29
CN201810273392.7A CN110317791A (zh) 2018-03-29 2018-03-29 Gmp级无血清悬浮细胞大规模生产慢病毒的方法
PCT/CN2019/080215 WO2019184996A1 (zh) 2018-03-29 2019-03-28 Gmp级无血清悬浮细胞大规模生产慢病毒的方法

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JPWO2019184996A5 JPWO2019184996A5 (https=) 2022-03-31
JP2021519108A5 JP2021519108A5 (https=) 2022-03-31

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JP2024051544A Pending JP2024075766A (ja) 2018-03-29 2024-03-27 Ｇｍｐレベルの無血清懸濁液細胞を用いるレンチウイルスの大規模製造方法

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US (3) US11845962B2 (https=)
EP (1) EP3778880A4 (https=)
JP (2) JP2021519108A (https=)
KR (1) KR20200136463A (https=)
CN (1) CN110317791A (https=)
AU (1) AU2019241301B2 (https=)
SG (1) SG11202009826PA (https=)
WO (1) WO2019184996A1 (https=)

Families Citing this family (6)

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Publication number Priority date Publication date Assignee Title
CN110317791A (zh) * 2018-03-29 2019-10-11 西比曼生物科技（香港）有限公司 Gmp级无血清悬浮细胞大规模生产慢病毒的方法
CN110894494B (zh) * 2019-11-22 2022-09-27 广西梧州制药（集团）股份有限公司 一种大规模高密度悬浮培养293细胞高产腺病毒的方法
CN113832112A (zh) * 2020-06-24 2021-12-24 重庆精准生物技术有限公司 一种制备慢病毒的混合方法及混合系统
EP4330381A1 (en) * 2021-04-27 2024-03-06 Novartis AG Viral vector production system
CN113265425A (zh) * 2021-05-08 2021-08-17 苏州博腾生物制药有限公司 基于悬浮细胞的car-cd19慢病毒制备工艺
CN114990077A (zh) * 2022-08-01 2022-09-02 深圳市先康达生命科学有限公司 一种慢病毒包装与纯化方法

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JP2014533516A (ja) * 2011-11-24 2014-12-15 ジェネトン 工業的な医薬用途に適合する拡張可能なレンチウイルスベクター製造システム

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WO2007095336A2 (en) 2006-02-15 2007-08-23 The Immune Response Corporation Production of hiv
PL2007883T3 (pl) 2006-04-20 2012-07-31 Wyeth Llc Sposób oczyszczania do izolacji oczyszczonego wirusa pęcherzykowatego zapalenia jamy ustnej z hodowli komórkowej
JP2011507523A (ja) 2007-12-21 2011-03-10 ワイス・エルエルシー 遺伝子改変した弱毒化水疱性口内炎ウイルス、組成物およびその使用方法
CN102203242B (zh) 2008-11-03 2013-06-12 克鲁塞尔荷兰公司 产生腺病毒载体的方法
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CN103881984A (zh) * 2013-12-06 2014-06-25 深圳市赛百诺基因技术有限公司 无血清悬浮细胞生产重组腺病毒的方法及其药品制剂生产方法
FR3014901B1 (fr) 2013-12-17 2017-06-09 Genethon Procede de purification de virus ou vecteurs viraux enveloppes
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Publication number Publication date
US20250084386A1 (en) 2025-03-13
AU2019241301B2 (en) 2025-04-17
EP3778880A4 (en) 2021-12-29
AU2019241301A1 (en) 2020-10-22
KR20200136463A (ko) 2020-12-07
WO2019184996A1 (zh) 2019-10-03
US20240060053A1 (en) 2024-02-22
US12180513B2 (en) 2024-12-31
US11845962B2 (en) 2023-12-19
EP3778880A1 (en) 2021-02-17
SG11202009826PA (en) 2020-11-27
CN110317791A (zh) 2019-10-11
US20210363497A1 (en) 2021-11-25
JP2024075766A (ja) 2024-06-04

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