KR20120099258A - 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 - Google Patents
화합물들의 효과적인 생산을 위한 미생물 엔지니어링 Download PDFInfo
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- KR20120099258A KR20120099258A KR20127015612A KR20127015612A KR20120099258A KR 20120099258 A KR20120099258 A KR 20120099258A KR 20127015612 A KR20127015612 A KR 20127015612A KR 20127015612 A KR20127015612 A KR 20127015612A KR 20120099258 A KR20120099258 A KR 20120099258A
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- gene
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- succinic acid
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| US9758771B2 (en) | 2013-06-25 | 2017-09-12 | Cj Cheiljedang Corporation | Microorganism with enhanced L-lysine productivity and method for producing L-lysine by using same |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2010349739B2 (en) * | 2009-11-18 | 2014-10-16 | Ptt Global Chemical Public Company Limited | Metabolic evolution of Escherchia coli strains that produce organic acids |
| US10138504B2 (en) | 2010-08-06 | 2018-11-27 | Lallemand Hungary Liquidity Management Llc | Production of malonyl-CoA derived products via anaerobic pathways |
| BR112014017686A2 (pt) * | 2012-01-30 | 2017-06-27 | Myriant Corp | produção de ácido mucônico a partir de micro-organismos geneticamente engenheirados |
| CN102994436A (zh) * | 2012-10-29 | 2013-03-27 | 江南大学 | 一种产减毒类脂a的大肠杆菌基因工程菌及其应用 |
| JP6085190B2 (ja) * | 2013-02-26 | 2017-02-22 | 花王株式会社 | 変異微生物及びそれを用いた有用物質の生産方法 |
| BR112016001416A2 (pt) * | 2013-07-23 | 2017-08-29 | Myriant Corp | Bactéria escherichia coli e método de produção de ácido succínico utilizando difusão facilitada para importação de açúcar |
| WO2015034948A1 (en) | 2013-09-03 | 2015-03-12 | Myriant Corporation | A process for manufacturing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol |
| CN104046586B (zh) * | 2014-07-02 | 2016-08-17 | 山东大学 | 一株基因工程菌及其在生产(2r,3r)-2,3-丁二醇中的应用 |
| US20170298363A1 (en) * | 2014-09-18 | 2017-10-19 | Genomatica, Inc | Non-natural microbial organisms with improved energetic efficiency |
| US10301613B2 (en) | 2015-09-15 | 2019-05-28 | Arizona Board Of Regents On Behalf Of Arizona State University | Targeted remodeling of prokaryotic genomes using CRISPR-nickases |
| JP7437116B2 (ja) | 2016-03-02 | 2024-02-22 | ピーティーティー グローバル ケミカル パブリック カンパニー リミテッド | 遺伝子操作された微生物からのムコン酸生成の改善 |
| EP3680241A1 (en) | 2017-03-20 | 2020-07-15 | Forma Therapeutics, Inc. | Pyrrolopyrrole compositions as pyruvate kinase (pkr) activators |
| CN111094570A (zh) | 2017-06-30 | 2020-05-01 | Ptt全球化学股份有限公司 | 具有稳定的功能dna序列的拷贝数的微生物及相关方法 |
| WO2020061255A1 (en) | 2018-09-19 | 2020-03-26 | Forma Therapeutics, Inc. | Activating pyruvate kinase r |
| US10675274B2 (en) | 2018-09-19 | 2020-06-09 | Forma Therapeutics, Inc. | Activating pyruvate kinase R |
| CN109251941B (zh) * | 2018-09-30 | 2020-11-06 | 江南大学 | 一种高产琥珀酸的大肠杆菌及其应用 |
| KR102129379B1 (ko) | 2018-10-10 | 2020-07-02 | 한국과학기술원 | 고활성의 말산 탈수소효소가 도입된 숙신산 생성용 변이 미생물 및 이를 이용한 숙신산 제조방법 |
| HUE072832T2 (hu) | 2019-09-19 | 2025-12-28 | Novo Nordisk Healthcare Ag | Piruvát kináz R-t (PKR) aktiváló készítmények |
| CN113278641B (zh) * | 2020-05-27 | 2022-06-21 | 安徽华恒生物科技股份有限公司 | 生产l-缬氨酸的重组大肠杆菌、其构建方法及其应用 |
| IT202000013243A1 (it) | 2020-06-04 | 2021-12-04 | Novamont Spa | Processo per la purificazione di una miscela di dioli |
| IT202000031979A1 (it) | 2020-12-22 | 2022-06-22 | Novamont Spa | Processo di purificazione di una miscela comprendente dioli e acetali |
| IT202100006419A1 (it) | 2021-03-17 | 2022-09-17 | Novamont Spa | Recupero di dioli da una miscela |
| US12128035B2 (en) | 2021-03-19 | 2024-10-29 | Novo Nordisk Health Care Ag | Activating pyruvate kinase R |
| IT202100030572A1 (it) | 2021-12-02 | 2023-06-02 | Novamont Spa | 1,3-butandiolo purificato da una miscela di dioli |
| CN118546852B (zh) * | 2024-07-30 | 2024-10-18 | 北京化工大学 | 一种高效共利用葡萄糖、木糖生产丁二酸的基因工程菌及其应用 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996034961A1 (en) | 1995-05-05 | 1996-11-07 | Genencor International, Inc. | Application of glucose transport mutants for production of aromatic pathway compounds |
| MY121380A (en) | 1998-04-13 | 2006-01-28 | Univ Georgia | Pyruvate carboxylase overexpression for enhanced production of oxaloacetate-derived biochemicals in microbial cells |
| US6989265B2 (en) | 2002-01-23 | 2006-01-24 | Wisconsin Alumni Research Foundation | Bacteria with reduced genome |
| US7303906B2 (en) | 2002-09-06 | 2007-12-04 | Wisconsin Alumni Research Foundation | Competent bacteria |
| US7371558B2 (en) | 2002-10-04 | 2008-05-13 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
| US7232665B2 (en) | 2002-12-19 | 2007-06-19 | E. I. Du Pont De Nemours And Company | Mutations affecting carotenoid production |
| DE10314618A1 (de) | 2003-04-01 | 2004-10-14 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung von Stämmen der Familie Enterobacteriaceae |
| CN1890377B (zh) | 2003-10-21 | 2013-06-05 | 嘉吉有限公司 | 生产莫纳甜和莫纳甜前体 |
| KR100576342B1 (ko) | 2004-02-05 | 2006-05-03 | 씨제이 주식회사 | ｇａｌＲ 유전자가 불활성화된 Ｌ-쓰레오닌 생성 미생물,그를 제조하는 방법 및 상기 미생물을 이용한Ｌ-쓰레오닌의 제조방법 |
| US20050181488A1 (en) | 2004-02-12 | 2005-08-18 | Akhverdian Valery Z. | Method for producing L-threonine using bacteria belonging to the genus Escherichia |
| JP4771437B2 (ja) | 2004-08-27 | 2011-09-14 | ライス ユニバーシティー | 増加したコハク酸産生を有する変異Ｅ．ｃｏｌｉ株 |
| CN101023178A (zh) | 2004-09-17 | 2007-08-22 | 莱斯大学 | 高琥珀酸产出细菌 |
| AU2005308101A1 (en) | 2004-11-26 | 2006-06-01 | Kyowa Hakko Kirin Co., Ltd. | Industrially useful microorganism |
| KR100630836B1 (ko) | 2005-04-08 | 2006-10-02 | 한국과학기술원 | 인-실리코 분석을 통한 균주 개량방법 |
| US7524660B2 (en) | 2005-05-05 | 2009-04-28 | E.I. Du Pont De Nemours And Company | Utilization of fructose in microbial production strains |
| ES2401339T3 (es) | 2005-08-10 | 2013-04-18 | University Of Florida Research Foundation, Inc. | Materiales y métodos para producción eficiente de ácido láctico |
| EP1915445A2 (en) | 2005-08-20 | 2008-04-30 | Scarab Genomics, LLC | Reduced genome e. coli |
| AU2006287257A1 (en) | 2005-09-09 | 2007-03-15 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of succinate |
| DE102005047596A1 (de) | 2005-10-05 | 2007-04-12 | Degussa Ag | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien |
| WO2007043327A1 (ja) | 2005-10-13 | 2007-04-19 | Kao Corporation | 新規枯草菌変異株 |
| WO2007115228A2 (en) * | 2006-03-31 | 2007-10-11 | Rice University | Anaerobic fermentation of glycerol |
| KR100885616B1 (ko) | 2006-06-26 | 2009-02-24 | 씨제이제일제당 (주) | 글리세롤을 이용한 아미노산의 생산 방법 |
| KR101631719B1 (ko) * | 2007-03-20 | 2016-06-24 | 유니버시티 오브 플로리다 리서치 파운데이션, 인크. | 유효한 숙시네이트 및 말레이트 제조를 위한 물질 및 방법 |
| WO2008116851A1 (en) | 2007-03-23 | 2008-10-02 | Metabolic Explorer | Metabolically engineered microorganism useful for the production of acetol |
| JP2010521190A (ja) | 2007-03-23 | 2010-06-24 | メタボリック エクスプローラー | １，２−プロパンジオールの製造に有用な、代謝的に操作された微生物 |
| US20110039327A1 (en) * | 2007-05-18 | 2011-02-17 | Aaron Adriaan Winkler | Organic acid production by fungal cells |
| US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| WO2009043372A1 (en) | 2007-10-02 | 2009-04-09 | Metabolic Explorer | Increasing methionine yield |
| WO2009061429A2 (en) * | 2007-11-06 | 2009-05-14 | Massachusetts Institute Of Technology | Global transcription machinery engineering targeting the rnap alpha subunit (rpoa) |
| EP2241630B1 (en) | 2007-12-06 | 2016-06-01 | Ajinomoto Co., Inc. | A method for producing an organic acid |
| WO2010051527A2 (en) | 2008-10-31 | 2010-05-06 | Gevo, Inc. | Engineered microorganisms capable of producing target compounds under anaerobic conditions |
| US20120058530A1 (en) * | 2009-04-02 | 2012-03-08 | University Of Florida Research Foundation Inc. | Engineering the pathway for succinate production |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9758771B2 (en) | 2013-06-25 | 2017-09-12 | Cj Cheiljedang Corporation | Microorganism with enhanced L-lysine productivity and method for producing L-lysine by using same |
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| EP2501797A2 (en) | 2012-09-26 |
| CN102666838B (zh) | 2018-04-10 |
| CN102666838A (zh) | 2012-09-12 |
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| WO2011063055A3 (en) | 2011-11-17 |
| BR112012011990A2 (pt) | 2015-09-29 |
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