BR112016001416A2 - Bactéria escherichia coli e método de produção de ácido succínico utilizando difusão facilitada para importação de açúcar - Google Patents

Bactéria escherichia coli e método de produção de ácido succínico utilizando difusão facilitada para importação de açúcar

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Publication number
BR112016001416A2
BR112016001416A2 BR112016001416A BR112016001416A BR112016001416A2 BR 112016001416 A2 BR112016001416 A2 BR 112016001416A2 BR 112016001416 A BR112016001416 A BR 112016001416A BR 112016001416 A BR112016001416 A BR 112016001416A BR 112016001416 A2 BR112016001416 A2 BR 112016001416A2
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Brazil
Prior art keywords
succinic acid
glucose
metabolism
transport
fructose
Prior art date
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BR112016001416A
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English (en)
Inventor
Rogers Yocum R
Hermann Theron
Gong Wei
Christopher Collard Andrew
Yu Xiaohui
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Myriant Corp
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Publication date
Application filed by Myriant Corp filed Critical Myriant Corp
Publication of BR112016001416A2 publication Critical patent/BR112016001416A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

BACTÉRIAS E MÉTODOS DE PRODUÇÃO DE ÁCIDO SUCCÍNICO E OUTROS PRODUTOS QUÍMICOS UTILIZANDO DIFUSÃO FACILITADA PARA IMPORTAÇÃO DE AÇÚCAR. A presente invenção refere-se à produção de ácido succínico e outros produtos químicos derivados de fosfoenolpiruvato (PEP) por fermentação com um micro-organismo em meio de fermentação contendo um ou mais açúcares importados para a célula por difusão facilitada. Preferencialmente, tais micro-organismos têm sua capacidade de utilizar glicose como uma fonte de carbono e energia aumentada e por meio de manipulação genética de cepas progenitoras que foram originalmente construídas com o objetivo de produzir um ou mais produtos químicos (por exemplo, ácido succínico e/ou um sal de ácido succínico) em uma escala comercial utilizando matérias-primas que incluem, por exemplo, glicose, frutose, ou sacarose. As manipulações genéticas da presente invenção envolvem a introdução de genes exógenos envolvidos no transporte e metabolismo de glicose ou frutose nas cepas progenitoras. Os genes envolvidos no transporte e metabolismo de glicose ou frutose também podem ser introduzidos em um micro-organismo antes de desenvolver o organismo para produzir um produto químico particular. Os genes envolvidos no transporte e metabolismo de sacarose também podem ser usados para aumentar ou aprimorar a eficiência de transporte e metabolismo de açúcar por cepas já conhecidas por terem alguma capacidade de utilização de glicose em fermentações biológicas.
BR112016001416A 2013-07-23 2014-07-22 Bactéria escherichia coli e método de produção de ácido succínico utilizando difusão facilitada para importação de açúcar BR112016001416A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361857300P 2013-07-23 2013-07-23
PCT/US2014/047696 WO2015013334A1 (en) 2013-07-23 2014-07-22 Method of producing succinic acid and other chemicals using facilitated diffusion for sugar import

Publications (1)

Publication Number Publication Date
BR112016001416A2 true BR112016001416A2 (pt) 2017-08-29

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BR112016001416A BR112016001416A2 (pt) 2013-07-23 2014-07-22 Bactéria escherichia coli e método de produção de ácido succínico utilizando difusão facilitada para importação de açúcar

Country Status (10)

Country Link
US (2) US20160160245A1 (pt)
EP (1) EP3030649B1 (pt)
JP (1) JP6649551B2 (pt)
KR (1) KR102266177B1 (pt)
CN (1) CN105452445B (pt)
BR (1) BR112016001416A2 (pt)
CA (1) CA2919012C (pt)
ES (1) ES2908342T3 (pt)
SA (1) SA518391513B1 (pt)
WO (1) WO2015013334A1 (pt)

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EP3342874A4 (en) * 2015-08-24 2019-07-10 Riken PROCESS FOR PRODUCING AROMATIC COMPOUND AND DERIVATIVE THEREOF
BR112018006473A2 (pt) * 2015-09-30 2018-11-21 The University Of Queensland cepa de propionibacterium isolada, cepa p. acidipropionici wgs 7, cepa de propionibacterium melhorada, cepa de p. acidipropionici isolada, cepa de p. acidipropionici f3e8, método para produzir ácido propiônico, nova cepa p. acidipropionici, método para produzir uma nova cepa propionibacterium e método para produzir uma nova cepa p. acidipropionici
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
JP7066977B2 (ja) * 2017-04-03 2022-05-16 味の素株式会社 Ｌ－アミノ酸の製造法
US11279955B2 (en) * 2017-05-19 2022-03-22 Basf Se Process for producing an organic compound
JP2020530271A (ja) 2017-06-30 2020-10-22 ピーティーティー グローバル ケミカル パブリック カンパニー リミテッド 安定化されたコピー数の機能性ｄｎａ配列を有する微生物及び関連方法
CN111067922A (zh) * 2018-10-19 2020-04-28 临床营养国际(马来西亚)私人有限公司 一种延缓骨骼和肌肉衰老迹象的化合物
CN111411062B (zh) * 2020-05-07 2021-10-01 广东省农业科学院植物保护研究所 一株抗生链霉菌及其代谢产物的制备以及其在抗病菌方面的应用

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Publication number Publication date
JP2016528893A (ja) 2016-09-23
SA518391513B1 (ar) 2021-07-12
EP3030649A4 (en) 2017-02-08
EP3030649B1 (en) 2022-02-23
KR102266177B1 (ko) 2021-06-17
CA2919012A1 (en) 2015-01-29
EP3030649A1 (en) 2016-06-15
ES2908342T3 (es) 2022-04-28
CN105452445B (zh) 2020-08-21
WO2015013334A1 (en) 2015-01-29
KR20160033210A (ko) 2016-03-25
US20200032301A1 (en) 2020-01-30
US10934565B2 (en) 2021-03-02
CA2919012C (en) 2022-10-25
US20160160245A1 (en) 2016-06-09
CN105452445A (zh) 2016-03-30
JP6649551B2 (ja) 2020-02-19

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B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]