KR102596577B1 - 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 - Google Patents

생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 Download PDF

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KR102596577B1
KR102596577B1 KR1020227023873A KR20227023873A KR102596577B1 KR 102596577 B1 KR102596577 B1 KR 102596577B1 KR 1020227023873 A KR1020227023873 A KR 1020227023873A KR 20227023873 A KR20227023873 A KR 20227023873A KR 102596577 B1 KR102596577 B1 KR 102596577B1
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dna
rna
capture surface
biological sample
microvesicles
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KR20220105172A (ko
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요한 칼 올로브 스코그
다니엘 엔덜
아파르나 라마찬드란
하오헹 얀
에밀리 버그호프
타이-펜 웨이
미켈 노어홀름
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엑소좀 디아그노스틱스, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Tires In General (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020227023873A 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법 Active KR102596577B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201462022538P 2014-07-09 2014-07-09
US62/022,538 2014-07-09
US201462079763P 2014-11-14 2014-11-14
US62/079,763 2014-11-14
US201562166890P 2015-05-27 2015-05-27
US62/166,890 2015-05-27
KR1020177003611A KR102421185B1 (ko) 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법
PCT/US2015/039760 WO2016007755A1 (en) 2014-07-09 2015-07-09 Methods for isolating microvesicles and extracting nucleic acids from biological samples

Related Parent Applications (1)

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KR1020177003611A Division KR102421185B1 (ko) 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법

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KR20220105172A KR20220105172A (ko) 2022-07-26
KR102596577B1 true KR102596577B1 (ko) 2023-10-30

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KR1020177003611A Active KR102421185B1 (ko) 2014-07-09 2015-07-09 생물학적 샘플로부터 미세소포체를 단리시키고 핵산을 추출하는 방법

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US (1) US10465183B2 (cg-RX-API-DMAC7.html)
EP (2) EP3623792B1 (cg-RX-API-DMAC7.html)
JP (2) JP6759182B2 (cg-RX-API-DMAC7.html)
KR (2) KR102596577B1 (cg-RX-API-DMAC7.html)
CN (2) CN107002075B (cg-RX-API-DMAC7.html)
AU (2) AU2015287763B2 (cg-RX-API-DMAC7.html)
CA (2) CA3084920C (cg-RX-API-DMAC7.html)
ES (2) ES2762677T3 (cg-RX-API-DMAC7.html)
IL (2) IL250000B (cg-RX-API-DMAC7.html)
SG (1) SG11201700146QA (cg-RX-API-DMAC7.html)
WO (1) WO2016007755A1 (cg-RX-API-DMAC7.html)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2835641C (en) * 2011-05-11 2019-05-28 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
US11268085B2 (en) 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
SG11201700146QA (en) 2014-07-09 2017-02-27 Exosome Diagnostics Inc Methods for isolating microvesicles and extracting nucleic acids from biological samples
CN105821031A (zh) * 2016-01-18 2016-08-03 昆明医科大学 一种二氯甲烷法提取dna的原理
JP2019513391A (ja) 2016-04-15 2019-05-30 エクソサム ダイアグノスティクス,インコーポレイティド 未分化リンパ腫キナーゼ(alk)核酸およびalk融合転写産物の血漿ベースの検出、並びに癌の診断と治療におけるそれらの使用
WO2017185086A1 (en) 2016-04-22 2017-10-26 Exosome Diagnostics, Inc. Devices and methods for in vivo capture of biological samples and nucleic acids therein
KR102516168B1 (ko) 2016-05-02 2023-03-30 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
ES2908932T3 (es) 2016-05-05 2022-05-04 Exosome Diagnostics Inc Perfilado de ácidos nucleicos de microvesícula y usos del mismo como características distintivas en diagnóstico de rechazo de trasplante renal
JP7037504B2 (ja) * 2016-05-13 2022-03-16 エクソサム ダイアグノスティクス,インコーポレイティド 生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法
EP3845665A1 (en) 2016-08-17 2021-07-07 The Regents Of The University Of California A novel immunoprobe-based method to assess organ injury status through a biofluid-based cell-free dna (cfdna) assay
WO2018052011A1 (ja) 2016-09-14 2018-03-22 東レ株式会社 セルフリーdnaの回収方法
US11396676B2 (en) 2016-10-21 2022-07-26 Exosome Diagnostics, Inc. Sequencing and analysis of exosome associated nucleic acids
WO2018102162A1 (en) 2016-11-30 2018-06-07 Exosome Diagnostics, Inc. Methods and compositions to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients
KR20190100174A (ko) * 2016-12-23 2019-08-28 엑소팜 리미티드 미세소포 및 엑소좀의 정제 또는 단리를 위한 방법 및 조성물
US20200149036A1 (en) 2017-01-02 2020-05-14 Exosome Diagnostics, Inc. Methods to distinguish rna and dna in a combined preparation
CA3048212A1 (en) * 2017-01-06 2018-07-12 Mantra Bio, Inc. Systems and methods for algorithmic extracellular vesicle population discovery and characterization
KR101875594B1 (ko) * 2017-01-13 2018-07-06 ㈜로제타엑소좀 금속을 이용한 세포밖 소포체의 분리 방법
GB201706680D0 (en) * 2017-04-27 2017-06-14 Ge Healthcare Uk Ltd Device and method for sample isolation
WO2018213392A1 (en) 2017-05-17 2018-11-22 Exosome Diagnostics, Inc. Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection
CN107267440A (zh) * 2017-06-09 2017-10-20 李刚 适用于外泌体提取的提取试剂及应用及其提取方法
EP3652314B1 (en) * 2017-07-12 2023-08-23 Illumina, Inc. Nucleic acid extraction materials and methods
EP3652315A4 (en) 2017-07-12 2021-09-01 Exosome Diagnostics, Inc. METHODS FOR ISOLATION AND ENRICHMENT OF EXTRACELLULAR VESICLE POPULATIONS DERIVED FROM BIOFLUIDS, AND ASSOCIATED METHODS OF USE
US11345957B2 (en) 2017-07-18 2022-05-31 Exosome Diagnostics, Inc. Methods of treating glioblastoma in a subject informed by exosomal RNA signatures
JP2020528766A (ja) * 2017-07-26 2020-10-01 ロゼッタ エクソソーム 陽イオンを利用した細胞外小胞体の分離方法
SG11202003588QA (en) * 2017-09-20 2020-05-28 Molecular Stethoscope Inc Methods and systems for detecting tissue conditions
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
WO2019132688A1 (ru) * 2017-12-26 2019-07-04 Общество С Ограниченной Ответственностью "Простагност" Способ и устройство для выделения внеклеточных везикул из биологических жидкостей с помощью каскадной ультрафильтрации
CN111770931B (zh) * 2018-02-20 2023-08-25 高丽大学校产学协力团 用于分离外泌体的多柱及外泌体分离方法
WO2019236853A1 (en) 2018-06-06 2019-12-12 Exosome Diagnostics, Inc. Methods for developing urine biomarkers and for detecting bladder cancer
CN112585446A (zh) 2018-06-18 2021-03-30 埃克波菲尔有限公司 用于纯化或分离微泡和外泌体的方法与组合物
CN108841777A (zh) * 2018-06-22 2018-11-20 北京恩泽康泰生物科技有限公司 基于静电吸附的胞外囊泡及其内含物的提取方法及装置
JP7426725B2 (ja) * 2018-10-30 2024-02-02 Craif株式会社 細胞外小胞を捕捉するために用いられるデバイス、細胞外小胞の保存方法および移送方法
EP3884045B1 (en) 2018-11-20 2023-01-11 Exosome Diagnostics, Inc. Methods for internal controls of microvesicle isolations
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
US12565644B2 (en) * 2019-08-08 2026-03-03 Biocartis Nv Nucleic acid purification chemistry
US12493029B2 (en) 2019-09-18 2025-12-09 Exosome Diagnostics, Inc. Compositions, methods, and kits for the isolation of extracellular vesicles
JP2023501354A (ja) * 2019-11-04 2023-01-18 ナサスビオテック、ソシエダッド、リミターダ 核酸の単離方法
CN114761556A (zh) * 2019-12-16 2022-07-15 凯杰有限公司 富集囊泡rna的方法
WO2021122846A1 (en) * 2019-12-16 2021-06-24 Qiagen Gmbh Enrichment method
EP4140568A4 (en) * 2020-04-24 2024-05-22 Toyobo Co., Ltd. Ion exchange membrane
CN115698265A (zh) * 2020-04-24 2023-02-03 高丽大学校算学协力团 微囊泡分离方法及微囊泡分离装置
AU2021281287A1 (en) 2020-05-29 2023-01-19 Bio-Techne Corporation Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
WO2022133159A1 (en) 2020-12-17 2022-06-23 Nephrosant, Inc. Kits for stabilization of urine samples
EP4355873A1 (en) * 2021-06-17 2024-04-24 QIAGEN GmbH Method for isolating non-vesicular mirna
US20250075258A1 (en) 2021-07-16 2025-03-06 Exosome Diagnostics, Inc. Methods of detecting sjogren's syndrome using salivary exosomes
DE102021208893B3 (de) 2021-08-13 2022-11-17 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Isolieren von Analyten unterschiedlicher Analyt-Klassen
WO2023076528A2 (en) * 2021-10-28 2023-05-04 10X Genomics, Inc. Methods for sample preparation
WO2023158869A1 (en) 2022-02-18 2023-08-24 Exosome Diagnostics, Inc. Use of microvesicle signatures in the identification and treatment of renal disorders
WO2024054572A1 (en) 2022-09-07 2024-03-14 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物
KR102784335B1 (ko) * 2023-02-09 2025-03-19 순천향대학교 산학협력단 세포유리핵산의 고농축화 분리 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014036391A1 (en) * 2012-08-30 2014-03-06 Exosome Diagnostics, Inc. Controls for nucleic acid assays
US20140093880A1 (en) * 2012-09-28 2014-04-03 Samsung Electronics Co., Ltd. Compositions, kits, and methods for isolating vesicles

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
JP3696238B2 (ja) * 1993-08-30 2005-09-14 プロメガ・コーポレイシヨン 核酸精製用組成物及び方法
FR2788780B1 (fr) * 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
ES2588227T3 (es) * 2006-10-10 2016-10-31 Trovagene, Inc. Composiciones, métodos y kits para aislar ácidos nucleicos de fluidos corporales usando medios de intercambio aniónico
AU2009212543B2 (en) 2008-02-01 2015-07-09 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
WO2010065765A2 (en) 2008-12-04 2010-06-10 Aethlon Medical, Inc. Affinity capture of circulating biomarkers
WO2010141862A2 (en) * 2009-06-05 2010-12-09 Mayo Foundation For Medical Education And Research Methods and materials for isolating exosomes
EP2576779B1 (en) 2010-06-01 2017-08-09 Qiagen GmbH Method for isolating and/or purifying nucleic acid(s)
WO2012006476A2 (en) * 2010-07-07 2012-01-12 Aethlon Medical, Inc. Methods and compositions for quantifying exosomes
WO2012024695A1 (en) * 2010-08-20 2012-02-23 Life Technologies Corporation Magnetic beads having surface glycoconjugates and use thereof
CA2817111C (en) * 2010-11-10 2019-03-05 Exosome Diagnostics, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
US20140004601A1 (en) 2010-12-20 2014-01-02 Agency For Science, Technology And Research Method of purifying exosomes
CA2835641C (en) * 2011-05-11 2019-05-28 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
EP2721179A4 (en) 2011-06-16 2014-10-01 Caris Life Sciences Luxembourg Holdings S A R L BIOMARKER COMPOSITIONS AND METHOD THEREFOR
KR20140005688A (ko) * 2012-07-06 2014-01-15 삼성전자주식회사 사용자 인터페이스 방법 및 장치
BR112015016136A2 (pt) 2013-01-03 2017-07-11 Exosome Diagnostics Inc métodos para isolar microvesículas
SG11201700146QA (en) 2014-07-09 2017-02-27 Exosome Diagnostics Inc Methods for isolating microvesicles and extracting nucleic acids from biological samples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014036391A1 (en) * 2012-08-30 2014-03-06 Exosome Diagnostics, Inc. Controls for nucleic acid assays
US20140093880A1 (en) * 2012-09-28 2014-04-03 Samsung Electronics Co., Ltd. Compositions, kits, and methods for isolating vesicles

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CN113699143A (zh) 2021-11-26
EP3623792B1 (en) 2021-09-01
AU2019250221A1 (en) 2019-11-07
CN107002075B (zh) 2021-07-30
ES2898254T3 (es) 2022-03-04
JP2017520264A (ja) 2017-07-27
JP7128866B2 (ja) 2022-08-31
ES2762677T3 (es) 2020-05-25
SG11201700146QA (en) 2017-02-27
JP6759182B2 (ja) 2020-09-23
IL250000A0 (en) 2017-03-30
US20170198280A1 (en) 2017-07-13
CN113699143B (zh) 2024-08-06
IL280307A (en) 2021-03-01
KR20170028432A (ko) 2017-03-13
CN107002075A (zh) 2017-08-01
IL250000B (en) 2021-02-28
EP3167062A4 (en) 2017-12-06
EP3167062A1 (en) 2017-05-17
KR102421185B1 (ko) 2022-07-14
CA3084920A1 (en) 2016-01-14
KR20220105172A (ko) 2022-07-26
AU2015287763A1 (en) 2017-02-23
US10465183B2 (en) 2019-11-05
EP3623792A1 (en) 2020-03-18
AU2015287763B2 (en) 2019-08-08
JP2020202860A (ja) 2020-12-24
IL280307B (en) 2022-02-01
WO2016007755A1 (en) 2016-01-14
EP3167062B1 (en) 2019-10-09
CA2954576C (en) 2020-08-11
CA3084920C (en) 2023-02-28
AU2019250221B2 (en) 2021-05-06
CA2954576A1 (en) 2016-01-14

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