KR102472071B1 - 대류 유체 디바이스에서의 핵산의 표면-기반 검출 - Google Patents

대류 유체 디바이스에서의 핵산의 표면-기반 검출 Download PDF

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KR102472071B1
KR102472071B1 KR1020187030052A KR20187030052A KR102472071B1 KR 102472071 B1 KR102472071 B1 KR 102472071B1 KR 1020187030052 A KR1020187030052 A KR 1020187030052A KR 20187030052 A KR20187030052 A KR 20187030052A KR 102472071 B1 KR102472071 B1 KR 102472071B1
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oligonucleotide
sequence
reaction chamber
dna
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KR20190021198A (ko
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드미트리 에이 코다코브
데이비드 장
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윌리엄 마쉬 라이스 유니버시티
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

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  • Chemical Kinetics & Catalysis (AREA)
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  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
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  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020187030052A 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출 Active KR102472071B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020227041286A KR102789837B1 (ko) 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662314909P 2016-03-29 2016-03-29
US62/314,909 2016-03-29
PCT/US2017/024530 WO2017172760A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acid in a convection flow fluidic device

Related Child Applications (1)

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KR1020227041286A Division KR102789837B1 (ko) 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출

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KR20190021198A KR20190021198A (ko) 2019-03-05
KR102472071B1 true KR102472071B1 (ko) 2022-11-29

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KR1020227041286A Active KR102789837B1 (ko) 2016-03-29 2017-03-28 대류 유체 디바이스에서의 핵산의 표면-기반 검출

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US (2) US20200324292A1 (https=)
EP (2) EP3436198B1 (https=)
JP (3) JP6998319B2 (https=)
KR (2) KR102472071B1 (https=)
CN (1) CN109477136A (https=)
AU (2) AU2017241766A1 (https=)
BR (1) BR112018070197A2 (https=)
CA (1) CA3019422A1 (https=)
DK (1) DK3436198T3 (https=)
ES (1) ES2920954T3 (https=)
IL (1) IL261980B2 (https=)
PH (1) PH12018502100A1 (https=)
PT (1) PT3436198T (https=)
RU (1) RU2018137800A (https=)
SG (1) SG11201808493QA (https=)
WO (1) WO2017172760A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102843431B1 (ko) * 2018-09-14 2025-08-08 윌리엄 마쉬 라이스 유니버시티 대류 가열 및 무-라벨 마이크로어레이를 사용하여 dna의 다중 증폭 및 검출을 위한 장치 및 방법
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法
CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法
DE102020212253A1 (de) * 2020-09-29 2022-03-31 SpinDiag GmbH Probenträger und Rotationsvorrichtung
EP4640315A1 (en) * 2024-04-26 2025-10-29 Appolon Bioteck Devices and methods for rapid nucleic acid amplification

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100056397A1 (en) * 2008-08-27 2010-03-04 Joo-Won Rhee Method of Producing Microarray Having Immobilized Double-Stranded Nucleic Acid Probe Including Double-Stranded Region and Single-Stranded Region

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027880A (en) 1995-08-02 2000-02-22 Affymetrix, Inc. Arrays of nucleic acid probes and methods of using the same for detecting cystic fibrosis
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
JP2003522963A (ja) * 2000-02-18 2003-07-29 アクララ バイオサイエンシーズ， インコーポレイテッド 複数部位反応デバイスおよび方法
US6586233B2 (en) 2001-03-09 2003-07-01 The Regents Of The University Of California Convectively driven PCR thermal-cycling
EP1437368B8 (en) 2001-10-16 2014-01-08 Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Nauki Institut Molekulyarnoi Biologi Im. V.A. Engelgardta Rossiiskoi Akademii Nauk Composition for polymerising immobilisation of biological molecules and method for producing said composition
DE60334471D1 (de) * 2002-10-11 2010-11-18 Univ Erasmus Primer für nukleinsäureamplifikation in pcr-basierten klonalitätsstudien
JP4517114B2 (ja) * 2003-03-28 2010-08-04 国立障害者リハビリテーションセンター総長 ｃＤＮＡ合成方法
KR100647289B1 (ko) * 2004-09-15 2006-11-23 삼성전자주식회사 마랑고니 대류를 이용한 ｐｃｒ 장치 및 이를 이용한ｐｃｒ 방법
US20060223122A1 (en) * 2005-03-08 2006-10-05 Agnes Fogo Classifying and predicting glomerulosclerosis using a proteomics approach
GB2424946A (en) * 2005-04-05 2006-10-11 Stratec Biomedical Systems Ag A detection system for substance binding using up-converting fluorescent probes
EP2458011B1 (en) * 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US8735103B2 (en) 2006-12-05 2014-05-27 Electronics And Telecommunications Research Institute Natural convection-driven PCR apparatus and method using disposable polymer chip
US8110353B2 (en) 2007-02-05 2012-02-07 California Institute Of Technology Engineered toehold reactions and networks
US8043810B2 (en) 2007-05-02 2011-10-25 Eagle Eye Research, Inc. Analyte detection using autocatalytic chain reactions
NZ582786A (en) * 2007-07-23 2012-09-28 Clondiag Gmbh Assays for nucleic acids
CN101983236B (zh) 2008-01-24 2014-03-12 基亚生物科技股份有限公司 热对流聚合酶连锁反应之方法及装置
US9074246B2 (en) * 2010-01-25 2015-07-07 Rd Biosciences, Inc. Self-folding amplification of target nucleic acid
US9506112B2 (en) * 2010-02-05 2016-11-29 Siemens Healthcare Diagnostics Inc. Increasing multiplex level by externalization of passive reference in polymerase chain reactions
PT2363502T (pt) * 2010-03-04 2017-05-17 Miacom Diagnostics Gmbh Fish multiplex melhorado
JP5848337B2 (ja) 2010-05-27 2016-01-27 エメラルド セラピューティクス，インコーポレーテッド 修飾した核酸を用いる情報伝播のシステムおよび方法
AU2011319755B2 (en) * 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
WO2012162161A1 (en) * 2011-05-20 2012-11-29 Phthisis Diagnostics Microsporidia detection system and method
US20140302486A1 (en) * 2011-09-02 2014-10-09 President And Fellows Of Harvard College Systems and methods for detecting biomarkers of interest
JP6427753B2 (ja) * 2013-09-11 2018-11-28 国立大学法人大阪大学 熱対流生成用チップ、熱対流生成装置、及び熱対流生成方法
KR102242192B1 (ko) * 2013-12-16 2021-04-20 윌리엄 마쉬 라이스 유니버시티 미세 조정된 초특정 핵산 하이브리다이제이션 프로브
JP6967006B2 (ja) * 2016-01-15 2021-11-17 クアンタポール， インコーポレイテッド 低減されたバックグラウンドの光学に基づくナノポア分析

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100056397A1 (en) * 2008-08-27 2010-03-04 Joo-Won Rhee Method of Producing Microarray Having Immobilized Double-Stranded Nucleic Acid Probe Including Double-Stranded Region and Single-Stranded Region

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RU2018137800A (ru) 2020-04-29
RU2018137800A3 (https=) 2020-06-18
AU2023203972A1 (en) 2023-07-13
ES2920954T3 (es) 2022-08-12
DK3436198T3 (en) 2022-06-27
JP6998319B2 (ja) 2022-01-18
EP3436198A4 (en) 2019-10-30
JP2019509751A (ja) 2019-04-11
CA3019422A1 (en) 2017-10-05
US20240278249A1 (en) 2024-08-22
IL261980B2 (en) 2023-08-01
KR20230004792A (ko) 2023-01-06
SG11201808493QA (en) 2018-10-30
WO2017172760A1 (en) 2017-10-05
EP3436198A1 (en) 2019-02-06
JP2022037151A (ja) 2022-03-08
KR20190021198A (ko) 2019-03-05
EP3436198B1 (en) 2022-05-04
EP4063014A1 (en) 2022-09-28
BR112018070197A2 (pt) 2019-01-29
CN109477136A (zh) 2019-03-15
JP2023184748A (ja) 2023-12-28
IL261980A (en) 2018-10-31
KR102789837B1 (ko) 2025-04-01
AU2017241766A1 (en) 2018-10-25
US20200324292A1 (en) 2020-10-15
PT3436198T (pt) 2022-07-13
IL261980B1 (en) 2023-04-01
PH12018502100A1 (en) 2019-07-29

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