CA3019422A1 - Surface-based detection of nucleic acids in convection flow fluidic devices - Google Patents

Surface-based detection of nucleic acids in convection flow fluidic devices Download PDF

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Publication number
CA3019422A1
CA3019422A1 CA3019422A CA3019422A CA3019422A1 CA 3019422 A1 CA3019422 A1 CA 3019422A1 CA 3019422 A CA3019422 A CA 3019422A CA 3019422 A CA3019422 A CA 3019422A CA 3019422 A1 CA3019422 A1 CA 3019422A1
Authority
CA
Canada
Prior art keywords
reaction chamber
oligonucleotide
sequence
dna
nucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3019422A
Other languages
English (en)
French (fr)
Inventor
Dmitriy A. Khodakov
David Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
William Marsh Rice University
Original Assignee
William Marsh Rice University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by William Marsh Rice University filed Critical William Marsh Rice University
Publication of CA3019422A1 publication Critical patent/CA3019422A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3019422A 2016-03-29 2017-03-28 Surface-based detection of nucleic acids in convection flow fluidic devices Pending CA3019422A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662314909P 2016-03-29 2016-03-29
US62/314,909 2016-03-29
PCT/US2017/024530 WO2017172760A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acid in a convection flow fluidic device

Publications (1)

Publication Number Publication Date
CA3019422A1 true CA3019422A1 (en) 2017-10-05

Family

ID=59965134

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3019422A Pending CA3019422A1 (en) 2016-03-29 2017-03-28 Surface-based detection of nucleic acids in convection flow fluidic devices

Country Status (16)

Country Link
US (2) US20200324292A1 (https=)
EP (2) EP3436198B1 (https=)
JP (3) JP6998319B2 (https=)
KR (2) KR102472071B1 (https=)
CN (1) CN109477136A (https=)
AU (2) AU2017241766A1 (https=)
BR (1) BR112018070197A2 (https=)
CA (1) CA3019422A1 (https=)
DK (1) DK3436198T3 (https=)
ES (1) ES2920954T3 (https=)
IL (1) IL261980B2 (https=)
PH (1) PH12018502100A1 (https=)
PT (1) PT3436198T (https=)
RU (1) RU2018137800A (https=)
SG (1) SG11201808493QA (https=)
WO (1) WO2017172760A1 (https=)

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KR102843431B1 (ko) * 2018-09-14 2025-08-08 윌리엄 마쉬 라이스 유니버시티 대류 가열 및 무-라벨 마이크로어레이를 사용하여 dna의 다중 증폭 및 검출을 위한 장치 및 방법
CN113257351A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种用于多碱基基因测序的基因文库及其构建方法
CN111879922A (zh) * 2020-07-13 2020-11-03 东南大学 适用于核酸及免疫同步检测集成纸基芯片结构及制造方法
DE102020212253A1 (de) * 2020-09-29 2022-03-31 SpinDiag GmbH Probenträger und Rotationsvorrichtung
EP4640315A1 (en) * 2024-04-26 2025-10-29 Appolon Bioteck Devices and methods for rapid nucleic acid amplification

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US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
JP2003522963A (ja) * 2000-02-18 2003-07-29 アクララ バイオサイエンシーズ， インコーポレイテッド 複数部位反応デバイスおよび方法
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KR20100025328A (ko) * 2008-08-27 2010-03-09 삼성전자주식회사 이중가닥 영역과 말단 단일가닥 영역을 포함하는 이중가닥 핵산 프로브가 고정된 마이크로어레이를 제조하는 방법
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Also Published As

Publication number Publication date
KR102472071B1 (ko) 2022-11-29
RU2018137800A (ru) 2020-04-29
RU2018137800A3 (https=) 2020-06-18
AU2023203972A1 (en) 2023-07-13
ES2920954T3 (es) 2022-08-12
DK3436198T3 (en) 2022-06-27
JP6998319B2 (ja) 2022-01-18
EP3436198A4 (en) 2019-10-30
JP2019509751A (ja) 2019-04-11
US20240278249A1 (en) 2024-08-22
IL261980B2 (en) 2023-08-01
KR20230004792A (ko) 2023-01-06
SG11201808493QA (en) 2018-10-30
WO2017172760A1 (en) 2017-10-05
EP3436198A1 (en) 2019-02-06
JP2022037151A (ja) 2022-03-08
KR20190021198A (ko) 2019-03-05
EP3436198B1 (en) 2022-05-04
EP4063014A1 (en) 2022-09-28
BR112018070197A2 (pt) 2019-01-29
CN109477136A (zh) 2019-03-15
JP2023184748A (ja) 2023-12-28
IL261980A (en) 2018-10-31
KR102789837B1 (ko) 2025-04-01
AU2017241766A1 (en) 2018-10-25
US20200324292A1 (en) 2020-10-15
PT3436198T (pt) 2022-07-13
IL261980B1 (en) 2023-04-01
PH12018502100A1 (en) 2019-07-29

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