KR102281806B1 - 3-hp를 생산할 수 있는 재조합 효모 및 이를 이용한 3-hp의 제조방법 - Google Patents

3-hp를 생산할 수 있는 재조합 효모 및 이를 이용한 3-hp의 제조방법 Download PDF

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KR102281806B1
KR102281806B1 KR1020150121623A KR20150121623A KR102281806B1 KR 102281806 B1 KR102281806 B1 KR 102281806B1 KR 1020150121623 A KR1020150121623 A KR 1020150121623A KR 20150121623 A KR20150121623 A KR 20150121623A KR 102281806 B1 KR102281806 B1 KR 102281806B1
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박재연
박우찬
박중민
서영빈
이상민
오자 메르자
코이비스토이넨 오우티
콘레이 앤드류
일멘 마르자
루오호넨 로라
조우텐 파울라
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KR1020150121623A 2014-08-29 2015-08-28 3-hp를 생산할 수 있는 재조합 효모 및 이를 이용한 3-hp의 제조방법 Active KR102281806B1 (ko)

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KR20140114505 2014-08-29
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KR20170025315A KR20170025315A (ko) 2017-03-08
KR102281806B1 true KR102281806B1 (ko) 2021-07-27

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US (1) US10704064B2 (enExample)
JP (1) JP6786477B2 (enExample)
KR (1) KR102281806B1 (enExample)
CN (1) CN107002019B (enExample)
BR (1) BR112017004069B1 (enExample)
DE (1) DE112015003962B4 (enExample)
WO (1) WO2016032279A1 (enExample)

Families Citing this family (13)

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CN108728471B (zh) * 2017-04-14 2020-01-31 中国科学院微生物研究所 产3-羟基丙酸的重组菌及其制备方法与应用
CN108118004B (zh) * 2017-12-15 2021-06-25 北京工商大学 一株喜仙人掌毕赤酵母在水果采后病害防治中的应用
WO2019152754A1 (en) * 2018-02-01 2019-08-08 Invista North America S.A.R.L. Methods and materials for the biosynthesis of beta hydroxy acids and derivatives and compounds related thereto
KR102140597B1 (ko) * 2018-04-17 2020-08-03 에스케이이노베이션 주식회사 에탄올 생산 경로가 억제된 내산성 효모 및 이를 이용한 젖산의 제조방법
KR102140596B1 (ko) * 2018-04-17 2020-08-04 에스케이이노베이션 주식회사 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법
KR102736070B1 (ko) * 2018-10-08 2024-12-02 에스케이이노베이션 주식회사 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
EP3959327A1 (en) * 2019-04-24 2022-03-02 Genomatica, Inc. Engineered trans-enoyl coa reductase and methods of making and using
KR20210041903A (ko) 2019-10-08 2021-04-16 에스케이이노베이션 주식회사 락테이트 대사 및 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210128742A (ko) * 2020-04-17 2021-10-27 에스케이이노베이션 주식회사 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210158676A (ko) 2020-06-24 2021-12-31 에스케이이노베이션 주식회사 젖산 생산능이 증가된 재조합 내산성 효모
CN114480232B (zh) * 2020-10-26 2024-02-02 北京化工大学 生产丙二酸的工程菌及其构建方法和应用
IL307636A (en) * 2021-04-13 2023-12-01 Debut Biotechnology Inc Bioproduction of flavonoids and anthocyanin using microbial hosts
CN113563410A (zh) * 2021-07-06 2021-10-29 南京瑞源生物技术有限公司 一种蛋白提取试剂配方

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100248233A1 (en) 2007-07-23 2010-09-30 Dsm Ip Assets B.V. Acetyl-coa producing enzymes in yeast
US20120135481A1 (en) 2010-11-22 2012-05-31 Novozymes, Inc. Compositions and methods for 3-hydroxypropionic acid production

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1575881B1 (en) 2002-01-18 2009-05-27 Novozymes A/S Alanine 2,3- aminomutase
JP5013448B2 (ja) * 2006-08-02 2012-08-29 帝人株式会社 出芽酵母の乳酸耐性又は生産性を向上させる多重遺伝子破壊の組み合わせ
MX2012003604A (es) 2009-09-27 2012-09-12 Opx Biotechnologies Inc Metodo para producir acido 3-hidroxipropionico y otros productos.
KR101860442B1 (ko) * 2011-06-27 2018-05-24 삼성전자주식회사 3-하이드록시프로피온산의 생산을 위한 유전자 조작

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100248233A1 (en) 2007-07-23 2010-09-30 Dsm Ip Assets B.V. Acetyl-coa producing enzymes in yeast
US20120135481A1 (en) 2010-11-22 2012-05-31 Novozymes, Inc. Compositions and methods for 3-hydroxypropionic acid production

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Metabolic Engineering, 22,104-109(2014)

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BR112017004069B1 (pt) 2024-02-06
WO2016032279A9 (en) 2016-04-28
KR20170025315A (ko) 2017-03-08
WO2016032279A1 (en) 2016-03-03
BR112017004069A2 (pt) 2018-01-23
DE112015003962T5 (de) 2017-08-03
JP6786477B2 (ja) 2020-11-18
DE112015003962B4 (de) 2024-12-19
CN107002019A (zh) 2017-08-01
US10704064B2 (en) 2020-07-07
US20170240932A1 (en) 2017-08-24
CN107002019B (zh) 2021-11-23
JP2017525372A (ja) 2017-09-07

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