KR20170025315A - 3-hp를 생산할 수 있는 재조합 효모 및 이를 이용한 3-hp의 제조방법 - Google Patents

3-hp를 생산할 수 있는 재조합 효모 및 이를 이용한 3-hp의 제조방법 Download PDF

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KR20170025315A
KR20170025315A KR1020150121623A KR20150121623A KR20170025315A KR 20170025315 A KR20170025315 A KR 20170025315A KR 1020150121623 A KR1020150121623 A KR 1020150121623A KR 20150121623 A KR20150121623 A KR 20150121623A KR 20170025315 A KR20170025315 A KR 20170025315A
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박재연
박우찬
박중민
서영빈
이상민
오자 메르자
코이비스토이넨 오우티
콘레이 앤드류
일멘 마르자
루오호넨 로라
조우텐 파울라
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Abstract

본 발명은 3-HP를 생산할 수 있는 재조합 효모및 이를 이용한 3-HP의 제조방법에 관한 것으로, 보다 구체적으로는, 외인성 AADH 유전자; 내인성 또는 외인성 ACC 유전자; 외인성 MCR 유전자; 및 외인성 HPDH 유전자를 함유하고, 아세트알데히드로부터 아세테이트를 경유하지 않고 직접 아세틸-CoA로 전환하는 대사경로 [피루브산 → 아세트알데히드 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP]를 통해 3-HP를 생산할 수 있는 재조합 효모 및 이를 이용한 3-HP 제조방법에 관한 것이다.

Description

3-HP를 생산할 수 있는 재조합 효모 및 이를 이용한 3-HP의 제조방법 {Recombinant Yeast Producing 3-Hydroxypropionic Acid and Method for Producing 3-Hydroxypropionic Acid Using the Same}
본 발명은 3-HP를 생산할 수 있는 재조합 효모 및 이를 이용한 3-HP의 제조방법에 관한 것으로, 보다 구체적으로는 AADH를 코딩하는 외인성 유전자; ACC를 코딩하는 내인성 또는 외인성 유전자; MCR을 코딩하는 외인성 유전자; 및 HPDH를 코딩하는 외인성 유전자를 함유하고, [피루브산 → 아세트알데히드 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP] 생합성 경로를 통해 3-HP를 생산할 수 있는 재조합 효모 및 이를 이용한 3-HP 제조방법에 관한 것이다.
3-HP (3-hydroxypropinoinc acid, C3)는 젖산(lactic acid, 2-hydroxypropionic acid)의 이성질체로써, 양말단에 카르복실기(carboxylic acid group)와 하이드록실기(hydroxyl group)을 가지고 있어 1,3-프로판디올, 아크릴산, 아크릴아마이드, 고분자(polymer) 등 다양한 화학물질로 전환이 가능한 유용한 물질이다. 실제로 이러한 이유 때문에 2004년 미국 에너지성(Deparment of Energy)은 3-HP를 바이오매스로부터 생산 가능한 유망한 화합물 중 하나로 선정한 바 있다. 특히 아크릴산은 코팅제, 접착제, 첨가제, 기저귀 등 시장성이 높은 물질로써 3-HP의 주된 응용처가 될 수 있다. 3-HP는 또한 발효를 통해 다양한 바이오매스 예를 들어 포도당으로부터 이론적으로 100% 수율로 생산할 수 있고, 미생물을 이용한 발효 공정은 최근의 친환경 및 재생가능 물질에 대한 요구를 수용하기에 적합하다.
3-HP를 바이오매스를 이용하여 상업적으로 생산한 사례는 아직 없으나, 다양한 방법으로의 연구가 진행되고 있으며, 경제성 있는 3-HP 생산 균주의 확보가 장애요인으로 대두되고 있다. 박테리아는 유기산을 생산하는 대표적인 미생물로 알려져 있으며, 식품 등의 산업에 유용하게 쓰이고 있다. 하지만, 대장균과 같은 대표적인 산업용 박테리아를 이용한 유기산 생산은 3-HP와 같은 대규모 화학 산업에 응용되기에는 단점이 있다. 유기산은 생산량이 증가할수록 수소화된 형태의 산이 증가하여 산성이 강해짐에 따라 산도가 올라가며(pH는 감소), 이는 대부분의 대장균의 활성을 떨어뜨린다. 대장균은 고농도의 유기산을 생산할때 산도를 중성으로 유지하기 위하여 수산화나트륨 (NaOH), 수산화암모늄 (NH4OH)와 같은 중화제(base)를 필요로 한다. 이는 투입되는 중화제에 따라 발효 공정의 비용을 증가시키고 추출 및 분리 과정을 어렵게 만들거나 비용을 크게 증가시키는 요인이 된다.
최근 유기산 생산의 화학산업에의 응용 사례에서는, 효모를 이용하여 포도당으로부터 유기산을 생산하고 있다. 효모는 박테리아에 비해 유기산에 대한 내성이 높아 높은 산도(낮은 pH)에서도 활성이 크게 저해되지 않으며, 유기산을 생산하는 모체(host)로써 더 적합하다고 볼 수 있다. 특히 효모는 전통적으로 주정, 산업용 에탄올 등을 생산하는 산업용 생촉매로써 널리 사용되어 왔으며 대량배양이 가능하고 박테리오파지에 대한 오염성 가능성이 없어 화학산업에서의 응용성이 박테리아 보다 더 좋다고 볼 수 있다.
유기산 생산에는 효모가 더 이점이 있으나, 3-HP를 비롯한 유기산 생산을 위한 효모의 활용에는 몇가지 단점이 있다. 우선, 효모를 유전적으로 개량하는 것은 박테리아보다 어려우며, 박테리아와 달리 미토콘드리아(mitochondria), 페록시좀(peroxisomes) 등과 같은 세포내 구조체에 의해 구분되는 세포의 형태 때문에, 특정 대사 효소를 발현하기 위해서는 함께 발현되는 대사 경로의 하위 세포 발현 위치 (sub-cellular location for expression)를 동일화해야 한다. 대표적인 대사 중간물질인 아세틸-CoA (Acetyl-CoA)의 경우 효모의 미토콘드리아에서 대부분 생성된다. 그러나, 목적 산물이 세포기질(cytosol)에서 생성되고 아세틸코에이를 거치는 경우라면 세포기질내에서 아세틸코에이를 생산하는 대응 방법이 필요하다. 또한, 균질 영역 (fungal kingdom)에 수많은 다른 효모가 존재하고, 모든 효모가 높은 생산성과 유기산에 대한 저항성, 대량배양의 요구를 충족하지 않으므로 유기산 생산에 적합한 모체(host)를 효과적으로 선별해야 한다.
효모는 육탄당인 포도당으로부터 효과적으로 에탄올을 생산하는 미생물로 잘 알려져 있으며, 일부 효모 종은 유기산을 생산한다. 미생물을 이용한 목적 산물을 생산하기 위한 개량 시에는 대사 반응에 대한 전체적인 산화ㅇ환원의 균형을 유지하는 것이 중요하며, 포도당으로부터 에탄올을 발효하는 대사 반응 또한 이러한 균형이 잘 유지되는 반응이다. 효모를 유전적으로 조작하여 유기산을 생산하는 경우에도 이러한 균형을 잘 유지해야 하며, 효모의 개량을 통해 젖산(lactic acid)을 생산하는 사례에서는 아세트알데히드(acetaldehyde)로부터 에탄올을 생산하는 환원 반응을 보완하기 위한 또다른 환원 반응의 LDH (lactic acid dehydrogenase)의 균형있는 도입이 중요함을 알 수 있다.
3-HP는 녹색 활주성 사상체 세균(Chloroflexus aurantiacus)과 같은 소수의 미생물로부터 소량 생산되는 것으로 알려져 있으며, 알칼리제네스 페칼리스(Alcaligenes faecalis)와 같은 미생물의 디메틸설포니오프로피네이트 (dimethylsulfoniopropinate)의 분해 과정이나 효모의 우라실(uracil)의 분해과정에서도 3-HP가 일부 발생하는 것으로 알려져 있다. 이와 같은 자연계에 존재하는 3-HP의 발견을 통해 3-HP 대사경로와 해당 효소에 대한 연구가 진행되어 왔으며 이를 바탕으로 최근에는 대장균에 3-HP 생합성에 필요한 유전자를 도입함으로써 3-HP를 생산하거나 이의 고분자(polymer) 형태인 PHA를 생산하는 연구가 진행되고 있다. 또한 이와 같이 자연계에서 단지 대사 중간 물질로 존재하거나 소량으로만 생산되는 3-HP의 생산성과 생산수율을 극대화하기 위하여 대사공학, 시스템생물학, 합성생물학 등의 기법이 활용되고 있다.
대사공학 기술의 발전에 따라 미생물을 이용한 다양한 물질의 생산 경로에 대한 예측이 가능해졌으며, 포도당으로부터 3-HP의 생산경로는 중간 대사물질에 따라 크게 아크릴로일-CoA (Acryloyl-CoA) 경로, β-알라닌 (β-alanine) 경로, 말로닐-CoA (Malonyl-CoA) 경로, 글리세롤 (Glycerol) 경로로 구분될 수 있다.
Acryloyl-CoA 경로는 포도당의 해당과정으로부터 얻어진 피루브산 또는 포스포에놀피루베이트(PEP)로부터 락테이트나 β-알라닌 등을 거쳐 아크릴로일-CoA로 변환된뒤, 이로부터 수화반응과 환원 반응을 통해 3-HP로 전환하는 대사경로를 말한다(피루브산 또는 PEP → 락테이트 또는 β-알라닌 → 아크릴로일-CoA → 3-HP). 아크릴로일-CoA는 프로피온산의 분해과정에서 발견되는 대사물질로써 물질에 도달하기 위한 깁스자유에너지값이 양수이므로 정반응이 비자발적인 반응이다. 또한 아크릴로일-CoA 티오에스터라아제의 기질에 대한 특이성이 떨어져 3-HP를 대량으로 생산하기 위한 대사경로로써 바람직하지 않다.
β-알라닌 경로는 피루브산 또는 옥살로아세테이트로부터 아미노기전이 (transamination) 반응을 통해 아미노산으로 전환된뒤 β-알라닌을 거쳐 다시 아미노기전이 반응을 통해 3-HP로 전환되는 대사경로를 말한다(피루브산 또는 옥살로아세테이트 → 아미노산 → β-알라닌 → 3-HP; 미국 공개특허공보 US 2012/0135481A1). β-알라닌이 3-HP로 아미노기전이되는 반응은 미생물에게 독성이 큰 말로네이트 세미알데히드를 거치게 되므로, 높은 활성을 가진 3-HP 디하이드로제나제를 필요로 한다. 또한 아미노기전이 반응은 일반적으로 정상상태 (steady-state)에서 아미노산 분자구조의 라디칼(radical) 형태를 유발하므로 이 반응의 효소는 라디칼의 반응성을 완화하기 위한 구조를 갖고 있다. 이 라디칼은 산소와의 강한 반응성을 보이므로 아미노기전이의 원활한 반응을 위해서는 반드시 혐기조건이나 라디칼분자의 안정화를 위한 보효소를 필요로 한다. 또한 아미노기전이 반응은 세포기질과 미토콘드리아 내부의 ATP 소비를 유발하여 에너지 소비관점에서도 바람직하지 않다.
말로닐-CoA 경로는 아세틸-CoA가 카복시화를 통하여 말로닐-CoA로 전환된뒤 환원반응을 통해 3-HP로 전환되는 대사경로이며(아세틸-CoA → 말로닐-CoA → 3-HP), 글리세롤 경로는 포도당으로부터 글리세롤로 전환된뒤, 글리세롤의 탈수반응에 의하여 3-하이드록시프로피온알데히드 (3-hydroxypropionaldehyde)를 거쳐 3-HP로 전환되는 대사경로이다(포도당 → 글리세롤 → 3-하이드록시프로피온알데히드 → 3-HP). 말로닐-CoA경로와 글리세롤 경로는 대장균을 비롯한 미생물이 일반적으로 생산하는 중간물질을 거치므로 3-HP 생산경로로써 가장 많이 연구되고 있다(미국 공개특허공보 US 2013/0071893 A1). 말로닐-CoA는 말로네이트 리덕타아제와 3-HP 디하이드로제나제, 글리세롤은 글리세롤 디하이드라타제와 알데히드 디하이드로제나제의 작용을 통해 3-HP로 전환될 수 있으므로 재조합 대장균을 이용하여 포도당이나 글리세롤을 이용하여 3-HP로 전환하는 방법이 잘 알려져 있다. 글리세롤 탈수는 아미노기전이와 마찬가지로 라디칼을 수반하는 반응으로써 산소가 존재하는 조건에서 반응하기 위해서는 보효소인 코엔자임 B12 (Coenzyme B12)를 필수로 필요로 한다.
산업용 발효의 관점에서 코엔자임 B12와 같은 보효소는 비용면에서 배지의 물질로 사용되기 어려우며, 효모와 같은 미생물은 해당 물질을 생합성하거나 세포내부로 흡수할 수 없으므로 효모를 이용하여 3-HP를 생산하기 위한 대사경로로써 β-알라닌 경로나 글리세롤 경로는 적합하지 않을 수 있다. 최근에는 이러한 문제점을 극복하기 위한 아미노기 전이 관여 효소에 대한 개량 연구가 알려진 바 있다 (미국 특허 제7,655,451호).
말로닐-CoA는 세포 기질내의 아세틸-CoA에서 합성되어 3-HP로 환원된다. 대장균과 같은 박테리아는 세포기질내에서 피루브산으로부터 아세틸-CoA가 생성되어 TCA 사이클이나 다른 대사 반응의 기질로써 사용된다. 하지만, 위에서 기술한 바와 같이 독립적인 소체 (sub-cellular compartments)를 가지고 있는 효모는 일반적으로 아세틸-CoA를 미토콘드리아에서 합성하여 TCA 사이클의 기질로써 사용하고 세포기질내의 아세틸-CoA는 에탄올 생성반응의 부반응인 아세테이트 생성반응이나 시트르산(citric acid) 순환 반응을 통해 생성된다. 아세테이트로부터 아세틸-CoA를 생성하거나 시트르산으로부터 아세틸-CoA를 얻는 반응은 모두 ATP를 소비하는 반응으로써, 박테리아에 비해 효모는 세포기질내 아세틸-CoA를 얻기 위해서는 에너지를 더 소비하게 되므로 에너지 활용 관점에서 불리할 수 있다.
효모는 세포기질의 환원상태나 단백질 합성후 접힘 (folding), 코돈 선호도 (codon usage) 등의 환경이 박테리아와 달라 박테리아 유래의 외래 효소를 발현할때, 그 활성이 나타나지 않거나 활성도가 크게 떨어지는 경우가 많다. 또한 산소나 기타 금속이온의 유무에 의한 활성도의 변화가 크므로 동일 기능을 갖는 외래 효소라 하더라도 그 효소의 유래에 따라 효모에서의 발현 결과는 달라질 수 있다. 실제로 xylose 대사의 중요한 효소인 자일로즈 이소머라제 (xylose isomerase; XI)의 경우 박테리아 유래의 XI는 효모에서 발현시 대부분 그 활성이 현저하게 낮은 결과를 보였으나, 혐기성 균류 (anaerobicfungus) 유래의 XI 도입을 통해 활성이 비교적 높은 자일로즈 대사가 가능하도록 효모의 개량에 성공한 것으로 알려져 있다. 따라서, 말로닐-CoA경로와 같이 박테리아나 고세균 (Archaea) 유래의 대사경로를 효모에 도입하는 경우에는 동일한 기능을 수행하는 다양한 유래의 유전자를 통해 활성도가 높은 유전자를 확보해야 한다.
다양한 효모 종 중 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 유전적으로 조작하여 3-HP를 제조하는 방법과 관련된 수많은 논문 및 특허가 존재하나, 사카로마이세스 세레비지애의 경우에는 [피루브산 → 아세트알데히드 → 아세트산 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP]의 경로를 거쳐 3-HP를 생성하는바, 3-HP 제조에 이르는 대사경로가 복잡하고 3-HP 생산능이 상대적으로 낮다는 문제점이 존재한다(미국 공개특허공보 US 2010/0248233A1; Y. Chen et al., Metabolic Engineering, 22:104-109, 2014).
이에, 본 발명자들은 상기 문제점을 해결하기 위하여 예의 노력한 결과, 세포기질 내에서 아세틸-CoA를 수득하는 과정에 있어서 ATP 소비가 발생하는 효모의 단점을 극복하기 위하여, 아세트알데히드로부터 아세테이트 중간체를 경유하지 않고 직접 아세틸-CoA로 전환하는 더 짧은 대사경로[피루브산 → 아세트알데히드 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP]를 착안하고, 상기 대사경로를 포함하는 재조합 효모를 이용하는 경우, 대장균을 이용하는 경우와 달리 pH 조절물질의 사용절감 및 이에 따른 염(salt) 생성 감소효과가 있을 뿐만 아니라, 낮은 pH에서도 바이오매스로부터 3-HP를 고농도 및 고수율로 생산할 수 있다는 것을 확인하고 본 발명을 완성하게 되었다.
본 발명의 목적은 아세트알데히드로부터 아세테이트를 경유하지 않고 직접 아세틸-CoA로 전환하는 대사경로 [피루브산 → 아세트알데히드 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP]를 통해 3-HP를 생산할 수 있는 재조합 효모를 제공하는데 있다.
본 발명의 다른 목적은 상기 재조합 효모를 이용하여 3-HP를 제조하는 방법을 제공하는데 있다.
상기 목적을 달성하기 위하여, 본 발명은 AADH를 코딩하는 외인성 유전자; ACC를 코딩하는 내인성 또는 외인성 유전자; MCR을 코딩하는 외인성 유전자; 및 HPDH를 코딩하는 외인성 유전자를 함유하고, [피루브산 → 아세트알데히드 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP]의 생합성 경로를 통해 3-HP를 생산할 수 있는재조합 효모를 제공한다.
본 발명에 있어서, 상기 효모는 사카로마이세스 속, 카자크스타니아 사카로마이세스 및 칸디다 속으로 구성된 군에서 선택되는 내산성을 가지는 효모일 수 있으며, 예를 들어 세레비지애 (Saccharomyces cerevisiae), 카자크스타니아 엑시구아 (Kazachstania exigua), 카자크스타니아 불데리 (Kazachstania bulderi), 및 칸디다 휴밀리스 (Candida humilis)로 구성된 군에서 선택되는 내산성을 가지는 효모일 수 있으나, 이에 제한되지 않는다.
본 발명은 또한, (a) 상기 재조합 효모를 적어도 하나의 탄소원을 함유하는 배지에서 배양하여, 3-HP를 제조하는 단계; 및 (b) 배양액으로부터 3-HP를 분리하는 단계를 포함하는 3-HP의 제조방법을 제공한다.
본 발명의 재조합 효모와 이를 이용한 3-HP 제조방법을 통해, 포도당 등 유용성이 있는 당으로부터 낮은 pH에서 3-HP를 고농도 및 고수율로 생산할 수 있는바, 바이오물질 유래 3-HP 및 이의 응용제품의 경제적인 산업적 생산에 크게 기여할 수 있다.
도 1은 본 발명에 따른 재조합 효모의 미토콘드리아에서 포도당으로부터 3-HP에 도달하는 대사경로 (변형된 말로닐-CoA 대사경로) 및 이에 관여하는 핵심 효소들을 나타낸 것이다;
도 2는 Acetyl-CoA carboxylase 효소의 ACC1 활성에 대한 상대적 순위를 나타낸 것이다;
도 3은 아키아 MCR 변이체의 상대적 활성(좌) 및 발현 수준 (SDS-PAGE: 우)을 확인한 결과를 나타낸 것이다;
도 4는 3-HP 경로 관련 효소를 발현시키기 위한 효모 발현 플라스미드인 pSK-084와 pSK-085를 나타낸 것이다;
도 5는 3-HP 생산에 영향을 미칠수 있는 배양 조건에 대한 테스트 결과를 나타낸 것이다; 및
도 6은 정립된 3-HP 생산 균주로 fed-batch (tablet spiking) 배양 조건을 사용하여 3-HP를 생산한 결과를 나타낸 것이다.
다른 식으로 정의되지 않는 한, 본 명세서에서 사용된 모든 기술적 및 과학적 용어들은 본 발명이 속하는 기술 분야에서 숙련된 전문가에 의해서 통상적으로 이해되는 것과 동일한 의미를 가진다. 일반적으로, 본 명세서에서 사용된 명명법은 본 기술 분야에서 잘 알려져 있고 통상적으로 사용되는 것이다.
일반적으로 효모에서 아세틸-CoA는 [아세트알데히드 → 아세트산 → 아세틸-CoA]의 대사경로를 거쳐 수득되고, 아세트산은 효모의 세포기질 내에서 생성되는바, 아세틸-CoA 수득과정에서 ATP가 AMP로 전환되어 에너지가 소비된다(도 1; Y. Chen etal., Metabolic Engineering, 22:104-109, 2014).
그러나, 본 발명에서는, 세포기질 내에서 아세틸-CoA를 수득하는 과정에 있어서, 아세트산을 거치지 않고, 아세트알데히드에서 직접 아세틸-CoA를 수득함으로써, ATP 소비가 발생하는 효모의 단점을 극복 및 개선시킨 대사경로(도 1)를 고안하여 적용하였다. 그 결과 본 발명의 재조합 효모를 이용하는 경우 낮은 pH에서도 포도당으로부터 3-HP를 고농도 및 고수율로 생산할 수 있음을 확인하였다.
따라서, 본 발명은 일 관점에서, AADH를 코딩하는 외인성 유전자; ACC를 코딩하는 내인성 또는 외인성 유전자; MCR을 코딩하는 외인성 유전자; 및 HPDH를 코딩하는 외인성 유전자를 함유하고, [피루브산 → 아세트알데히드 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP]의 생합성 경로를 통해 3-HP를 생산할 수 있는 재조합 효모에 관한 것이다.
상기 [피루브산 → 아세트알데히드 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP]의 3-HP 생합성 경로는 포도당 등의 탄소원으로부터 3-HP를 생성하는 경로에 해당하는 것으로써, (i) "피루브산 → 아세트알데히드"는 피루베이트 디카르복실라아제(Pyruvate decarboxylase; PDC)를 이용하여 중간체 생성없이 피루브산으로부터 아세트알데히드를 생합성하는 경로를 의미하고; (ii) "아세트알데히드 → 아세틸-CoA"는 아세틸기를 함유한 아세트알데히드 디하이드로제나제(Acetylating acetaldehyde dehydrogenase; AADH)를 이용하여 아세트알데히드로부터, 아세테이트와 같은 중간체를 경유하지 않고, 직접 아세틸-CoA를 생합성하는 경로를 의미하며; (iii) "아세틸-CoA → 말로닐-CoA"는 아세틸-CoA 카르복실라아제(Acetyl-CoA carboxylase; ACC)를 이용하여 중간체 생성없이 아세틸-CoA로부터 말로닐-CoA를 생합성하는 경로를 의미하고; (iv) "말로닐-CoA →→ 3-HP 또는 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP"는 2기능성(bifunctional) 말로닐-CoA 리덕타아제(Malonyl-CoA reductase; MCR)를 이용하여 중간체 생성없이 말로닐-CoA로부터 3-HP를 생합성하는 경로 또는 단일기능성(mono-functional) 말로닐-CoA 리덕타아제를 이용하여 말로네이트 세미알데히드를 생합성한 후 3-HP 탈수소효소(3-HP dehydrogenase)를 이용하여말로네이트 세미알데히드로부터 3-HP를 생합성하는 경로를 의미한다 (도 1).
본 발명에 있어서, PDC 유전자는 특별한 조작을 가하지 않았으나, 당업계에 알려진 공지의 기술을 적용하여, 효모에 내재된 PDC 유전자를 증폭시키는 등 3-HP 생성효율을 높이기 위한 PDC 유전자 조작을 할 수도 있다.
본 발명의 일 실시예에서는, 경로 효소 후보군에 대한 생물정보학적 게놈 마이닝(genome mining)을 통하여, 특정한 기능을 가지는 경로 효소를 인코딩하는 유전자를 추출해내었다.
본 발명에 있어서, 재조합 효모는 AADH를 코딩하는 외인성 유전자를 포함한다. 하나의 실시예에서 AADH를 코딩하는 유전자는 하기 표 1 내지 3에 기재된 AADH 아미노산 서열들로 구성된 군에서 선택되는 AADH 아미노산 서열과 60% 이상, 바람직하게 70% 이상, 80% 이상, 90% 이상, 91% 이상, 92% 이상, 93% 이상, 94% 이상, 95% 이상, 96% 이상, 97% 이상, 99% 이상 또는 100%의 상동성을 가지는 아미노산 서열의 AADH를 인코딩하는 핵산인 것을 특징으로 할 수 있으나, 아세트알데히드로부터 아세틸-CoA를 생합성하는 기능을 가지는 한 특별한 제한은 없다.
[표 1] AADH (adhE 타입)
Figure pat00001
[표 2] AADH (eutE 타입)
Figure pat00002

[표 3] AADH
Figure pat00003

본 발명에 있어서, 재조합 효모는 ACC를 코딩하는 유전자를 포함한다. 하나의 실시예에서 ACC를 코딩하는 유전자는 하기 표 4에 기재된 ACC 아미노산 서열들로 구성된 군에서 선택되는 ACC 아미노산 서열과 60% 이상, 바람직하게 70% 이상, 80% 이상, 90% 이상, 91% 이상, 92% 이상, 93% 이상, 94% 이상, 95% 이상, 96% 이상, 97% 이상, 99% 이상 또는 100%의 상동성을 가지는 아미노산 서열의 ACC를 인코딩하는 핵산인 것을 특징으로 할 수 있으나, 아세틸-CoA로부터 말로닐-CoA를 생합성하는 기능을 가지는 한 특별한 제한은 없다.
[표 4] ACC (진핵 멀티도메인 타입)
Figure pat00004
본 발명에 있어서, 재조합 효모는 MCR을 코딩하는 외인성 유전자를 포함한다. 하나의 실시예에서 MCR은 말로닐-CoA의 말로네이트 세미알데히드로의 전환과 말로네이트 세미알데히드의 3-HP로의 전환의 2기능성(bi-functional)을 가지는 것; 또는 말로닐-CoA의 말로네이트 세미알데히드로의 전환의 단일기능성(mono-functional)을 가지는 것을 특징으로 할 수 있다.
하나의 실시예에서, 상기 2기능성 MCR을 코딩하는 유전자는 표 5에 기재된 MCR 아미노산 서열들로 구성된 군에서 선택되는 MCR 아미노산 서열과 60% 이상, 바람직하게 70% 이상, 80% 이상, 90% 이상, 91% 이상, 92% 이상, 93% 이상, 94% 이상, 95% 이상, 96% 이상, 97% 이상, 99% 이상 또는 100%의 상동성을 가지는 아미노산 서열의 2기능성 MCR을 인코딩하는 핵산인 것을 특징으로 할 수 있으나, 말로닐-CoA로부터 말로네이트 세미알데히드의 생합성하는 기능과 말로네이트 세미알데히드로부터 3-HP의 생합성하는 기능을 동시에 가지는 한 특별한 제한은 없다.
[표 5] 2기능성 MCR
Figure pat00005

또 다른 실시예에서, 상기 MCR이 말로닐-CoA의 말로네이트 세미알데히드로의 전환의 단일기능성(mono-functional)을 가지는 경우에는, 말로네이트 세미알데히드를 3-HP로 전환시킬 수 있는 효소를 코딩하는 유전자를 추가로 함유하는 것을 특징으로 할 수 있다.
본 발명에 있어서, 상기 단일기능성 MCR을 코딩하는 유전자는 표 6에 기재된 MCR 아미노산 서열들로 구성된 군에서 선택되는 MCR 아미노산 서열과 60% 이상, 바람직하게 70% 이상, 80% 이상, 90% 이상, 91% 이상, 92% 이상, 93% 이상, 94% 이상, 95% 이상, 96% 이상, 97% 이상, 99% 이상 또는 100%의 상동성을 가지는 아미노산 서열의 단일기능성 MCR을 인코딩하는 핵산인 것을 특징으로 할 수 있으나, 말로닐-CoA로부터 말로네이트 세미알데히드의 생합성 기능을 가지는 한 특별한 제한은 없다.
[표 6] 단일 기능성 MCR
Figure pat00006

본 발명에 있어서, 상기 말로네이트 세미알데히드를 3-HP로 전환시킬 수 있는 효소효소를 코딩하는 유전자는 표 7 내지 표 10에 기재된 HPDH 아미노산 서열들, HIBADH 아미노산 서열들, HBDH 아미노산 서열들 및 BDH 아미노산 서열들로 구성된 군에서 선택되는 HPDH 아미노산 서열, HIBADH 아미노산 서열, HBDH 아미노산 서열 또는 BDH 아미노산 서열과 60% 이상의 상동성을 가지는 아미노산 서열의 효소를 인코딩하는 핵산인 것을 특징으로 할 수 있으나, 말로네이트 세미알데히드로부터 3-HP의 생합성 기능을 가지는 한 특별한 제한은 없다.
하기 표 7에 기재된 HPDH 아미노산 서열, 표 8에 기재된 HIBADH 아미노산 서열, 표 9에 기재된 HBDH 아미노산 서열, 및 표 10에 기재된 BDH 아미노산 서열은 모두 말로네이트 세미알데히드로부터 3-HP를 생합성하는 기능을 가지는 것이다.
[표 7] HPDH
Figure pat00007
Figure pat00008
[표 8] HIBADH
Figure pat00009

[표 9] HBDH-인코딩 유전자
Figure pat00010

[표 10] BDH-인코딩 유전자
Figure pat00011

본 발명에 따른 구체적 실시예에서, 3-HP 생산 경로를 가지는 재조합 효모는 표 11에 기재된 바와 같이 예를 들어, 서열번호 74-89로 구성된 군에서 선택된 아미노산 서열을 가지는 AADH를 코딩하는 외인성 유전자, 서열번호 99-106으로 구성된 군에서 선택된 아미노산 서열을 가지는 ACC를 코딩하는 내인 또는 외인성 유전자, 서열번호 107-116으로 구성된 군에서 선택된 아미노산 서열을 가지는 MCR을 코딩하는 외인성 유전자 및 서열번호 117-144로 구성된 군에서 선택된 아미노산 서열을 가지는 HPDH를 코딩하는 외인성 유전자를 포함할 수 있다.
[표 11]
Figure pat00012
[
Figure pat00013
Figure pat00014
본 발명에 있어서, 재조합 효모가 내산성이 있는 것을 특징으로 할 수 있고, 본 발명에 적합한 내산성 재조합 효모를 제작하기 위해서는 유기산(특히, 3-HP 및/또는 3-HP 생산시 부산물로 생성되는 유기산)에 내산성을 가지는 숙주효모를 사용하는 것이 바람직하다.
상기 내산성 효모는 사카로마이세스 속, 카자크스타니아 사카로마이세스 및 칸디다 속으로 구성된 군에서 선택되는 내산성을 가지는 효모일 수 있으며, 예를 들어 사카로마이세스 세레비지애 (Saccharomyces cerevisiae), 카자크스타니아 엑시구아 (Kazachstania exigua), 카자크스타니아 불데리 (Kazachstania bulderi), 및 칸디다 휴밀리스 (Candida humilis)로 구성된 군에서 선택된 것을 특징으로 할 수 있으나, 이에 한정되는 것은 아니다.
'내산성 효모'란 3-HP 등의 유기산에 대한 저항성을 가지는 효모를 의미하는 것으로, 내산성은 다양한 농도의 유기산을 포함하는 배지에서의 생장을 평가하여 확인할 수 있다. 즉, '내산성 효모'란 고농도의 유기산을 포함하는 배지 내에서 일반 효모에 비하여 높은 생장률 및 바이오매스 소모율을 나타내는 효모를 의미한다.
본 발명에 있어서,'내산성 효모'란 유기산(특히, 3-HP)의 pKa 값 미만의 pH에서, 배지에 유기산이 함유되지 않은 경우에 비해, 배지에 1M 이상의 유기산(특히, 3-HP)이 함유된 경우, 적어도 10%의 바이오매스 소모율 (당소모율 등) 또는 적어도 10%의 비생장률을 유지할 수 있는 효모로 정의한다. 보다 구체적으로, 본 발명에서,'내산성 효모'란 pH 7인 경우에 비해, pH 2~4에서 적어도 10%의 바이오매스 소모율 (당소모율 등) 또는 적어도 10%의 비생장률을 유지할 수 있는 효모로 정의한다. (2안)
본 발명에 따른 재조합효모는 통상의 방법에 따라 상기 유전자를 숙주 효모의 염색체(chromosome) 상에 삽입시키거나, 상기 유전자를 포함하는 벡터를 숙주 효모에 도입시킴으로써 제조할 수 있다.
상기 숙주효모는 DNA의 도입효율이 높고, 도입된 DNA의 발현 효율이 높은 숙주세포가 통상적으로 사용되며, 본 발명의 일 실시예에서는 내산성 효모를 이용하였으나, 이에 한정되는 것은 아니고, 목적 DNA가 충분히 발현될 수 있는 것이라면 어떠한 종류의 효모라도 무방하다.
상기 재조합효모는 임의의 형질전환(transformation) 방법에 따라 제조할 수 있다. "형질전환"이란 DNA를 숙주로 도입하여 DNA가 염색체의 인자로서 또는 염색체 통합완성에 의해 복제가능하게 되는 것으로 외부의 DNA를 세포 내로 도입하여 인위적으로 유전적인 변화를 일으키는 현상을 의미하며, 일반적인 형질전환 방법에는 전기천공법(electroporation), 초산 리튬-PEG법 등이 있다.
또한, 본 발명에서 유전자를 숙주 미생물의 염색체 상에 삽입하는 방법으로는 통상적으로 알려진 임의의 유전자 조작방법을 사용할 수 있으며, 일 예로는 레트로바이러스벡터, 아데노바이러스벡터, 아데노-연관 바이러스벡터, 헤르페스심플렉스 바이러스벡터, 폭스바이러스벡터, 렌티바이러스벡터, 비바이러스성벡터 등을 이용하는 방법이 있다. "벡터"는 적합한 숙주 내에서 DNA를 발현시킬 수 있는 적합한 조절 서열에 작동가능하게 연결된 DNA 서열을 함유하는 DNA 제조물을 의미한다. 벡터는 플라스미드, 파지 입자 또는 간단하게 잠재적 게놈 삽입물 일 수 있다. 적당한 숙주로 형질전환되면, 벡터는 숙주게놈과 무관하게 복제하고 기능할 수 있거나, 또는 일부경우에 게놈 그 자체에 통합될 수 있다. 플라스미드가 현재 벡터의 가장 통상적으로 사용되는 형태이다.
전형적인 플라스미드 벡터는 (a) 숙주세포당 플라스미드 벡터를 포함하도록 복제가 효율적으로 이루어지도록하는 복제 개시점, (b) 플라스미드 벡터로 형질전환된 숙주세포가 선발될 수 있도록 하는 항생제 내성 유전자 또는 영양 요구 마커 유전자 (auxotrophic marker gene) 및 (c) 외래 DNA 절편이 삽입될 수 있도록 하는 제한효소 절단부위를 포함하는 구조를 지니고 있다. 적절한 제한효소 절단부위가 존재하지 않을지라도, 통상의 방법에 따른 합성 올리고뉴클레오타이드어댑터(oligonucleotide adaptor) 또는 링커(linker)를 사용하면 벡터와 외래 DNA를 용이하게 라이게이션(ligation) 할 수 있다.
아울러, 상기 유전자는 다른 핵산 서열과 기능적 관계로 배치될 때 "작동가능하게 연결(operably linked)"된다. 이것은 적절한 분자(예를 들면, 전사 활성화 단백질)가 조절 서열(들)에 결합될 때 유전자 발현을 가능하게 하는 방식으로 연결된 유전자 및 조절 서열(들)일 수 있다. 예를 들면, 전서열(pre-sequence) 또는 분비리더(leader)에 대한 DNA는 폴리펩타이드의 분비에 참여하는 전단백질로서 발현되는 경우 폴리펩타이드에 대한 DNA에 작동가능하게 연결되고; 프로모터 또는 인핸서는 서열의 전사에 영향을 끼치는 경우 코딩서열에 작동가능하게 연결되거나; 또는 리보좀 결합 부위는 서열의 전사에 영향을 끼치는 경우 코딩 서열에 작동가능하게 연결되거나; 또는 리보좀 결합 부위는 번역을 용이하게 하도록 배치되는 경우 코딩 서열에 작동가능하게 연결된다.
일반적으로 "작동가능하게 연결된"은 연결된 DNA 서열이 접촉하고, 또한 분비 리더의 경우 접촉하고 리딩 프레임 내에 존재하는 것을 의미한다. 그러나, 인핸서(enhancer)는 접촉할 필요가 없다. 이들 서열의 연결은 편리한 제한 효소 부위에서 라이게이션 (연결)에 의해 수행된다. 그러한 부위가 존재하지 않는 경우, 통상의 방법에 따른 합성 올리고뉴클레오티드 어댑터(oligonucleotide adaptor) 또는 링커(linker)를 사용한다.
물론 모든 벡터가 본 발명의 DNA 서열을 발현하는데 모두 동등하게 기능을 발휘하지는 않고, 마찬가지로 모든 숙주가 동일한 발현 시스템에 대해 동일하게 기능을 발휘하지는 않는다. 그러나, 당업자라면 과도한 실험적 부담없이 본 발명의 범위를 벗어나지 않는 상태에서, 다른 여러 벡터, 발현 조절 서열 및 숙주 중에서 적절한 선택하여 적용할 수 있다. 예를 들어, 벡터를 선택함에 있어서는 숙주를 고려하여야 하는데, 이는 벡터가 그 안에 서 복제되어야만 하기 때문이고, 벡터의 복제 수, 복제 수를 조절할 수 있는 능력 및 당해 벡터에 의해 코딩되는 다른 단백질, 예를 들어 항생제 마커의 발현도 또한 고려되어야만 한다.
본 발명은 다른 관점에서, (a) 상기 재조합 효모를 적어도 하나의 탄소원을 함유하는 배지에서 배양하여, 3-HP를 생성시키는 단계; 및 (b) 상기 생성된 3-HP를 회수하는 단계를 포함하는 3-HP의 제조방법에 관한 것이다.
본 발명에 있어서, 탄소원은 글루코즈, 자일로즈, 아라비노즈, 수크로즈, 프룩토즈, 셀룰로오즈, 갈락토오스, 글루코즈 올리고머 및 글리세롤로 구성된 군에서 선택된 하나 이상인 것을 특징으로 할 수 있으나, 이에 한정되는 것은 아니다.
본 발명에 있어서, 배양은 미생물 예를 들어 대장균 등이 더 이상 작용하지 못하도록 (예를 들어, 대사체 생산 불가능) 하는 조건에서 이루어질 수 있다. 예를 들어, 배양은 pH 1.0 내지 6.5, 바람직하게 pH 1.0 내지 6.0, 보다 바람직하게는 2.6 내지 4.0인 것을 특징으로 할 수 있으나, 이에 한정되는 것은 아니다.
실시예
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.
실시예 1: 3-HP 내성 기반, 모체 효모 균주의 선별
3-HP 생성 개체의 필수적인 특징은 고농도의 3-HP에 대한 상당한 내성을 가져, 균주 능력의 최소한의 손실만으로 발효 시 산물을 축적할 수 있다는 것이다. 낮은 pH에서 고농도의 3-HP에 대한 내성을 가지는지 및 상기 조건 하에서 성장하고 포도당 대사할 수 있는지 확인하기 위하여, 718개의 다양한 야생형 효모 균주를 조사하였다(표 12).
전체 균주 세트의 내산성(acid tolerance)을 조사하기 위하여, 성장 분석(growth assays)을 근거로 한, 아가 플레이트 및 액체 배지 마이크로타이터(microtitre) 플레이트를 처음에 사용하였다. 그 후, 쉐이크 플라스크 배양액(shake flask cultivations) 내 균주의 서브세트에 대한 낮은 pH에 과량의 3-HP에 대한 내성을 평가하였다. 분리 시, 상기 조사 방법 중 어떤 것도 산업 현장에서 3-HP 내성의 완벽한 지표가 아니었으나, 다양한 방법의 조합이 유망한 3-HP 생산 효모 균주 제조에 대한 빈틈없고 탄탄한 수단을 제공한다.
우선, 다양한 양의 3-HP를 함유하는 고체 YPD-기반 아가 배지에서 718개의 효모 균주의 성장을 평가하였다: 0 g/L 3-HP (pH 6.62), 50 g/L 3-HP (pH 3.44), 75 g/L 3-HP (pH 3.28), 100 g/L 3-HP (pH 3.17) 및 125 g/L 3-HP (pH 3.08). 그 후, 다양한 양의 3-HP에 대한 내성을 근거로, 각각의 균주의 점수를 매겼다.
그 후, 자동으로 배양하고, 흔들고, 배양액의 혼탁도를 측정하는 BIOSCREEN C 기계를 이용하여, 마이크로타이터 플레이트 액체 배양액 내 3-HP의 부재(SCD-기반 배지, 0 g/L 3-HP, pH 6.0) 또는 존재(SCD-기반 배지, 70 g/L 3-HP, pH 3.5) 시 718개의 효모 균주의 성장을 평가하였다. 각각의 실험 조건 하에서, 각각의 균주의 유도기 (lag phase), 최대 성장률 및 최종 세포 밀도를 만들기 위하여, 미생물 성장곡선 모델링을 위한 현존하는 소프트웨어를 적용하였다. 상기 분석 방법 동안, 3-HP 부재 시 최대 성장률, 3-HP 존재 시 최대 성장률 및 상기 두 개의 최대 성장률 값의 상대오차를 근거로, 각각의 균주의 점수를 매겼다.
액체 조작 로봇을 이용하여, 마이크로타이터 플레이트 내 85 g/L 3-HP (pH 3.5)를 함유하는 YPD-기반 액체 배지 내에서 718개의 효모 균주의 성장률 및 포도당 이용률을 평가하였다. 성장 플레이트를 배양시키고, 지정된 시간(time-points)에 OD 측정을 위한 샘플을 희석하고, 지정된 시간에 잔여 포도당의 양을 측정하기 위한 HPLC 분석을 위한 상청액을 모으기 위하여, 자동화된 작업흐름(work-flow)을 이용하였다. BIOSCREEN C 성장 분석과 반대로, 로봇의 마이크로타이터 플레이트 성장 분석은 상기 두 가지 방법과 같이 단지 성장률만을 평가하는 대신에, 포도당 이용률을 평가케하며, 더 많은 통기(aeration) 및 더 높은 최대 세포 밀도를 가지게 한다. 이러한 조사 방법 동안, 3-HP 존재 시 최대 세포 밀도 및 낮은 pH에서 3-HP 존재 시 포도당 소비능을 근거로 각각의 균주의 점수를 매겼다.
다양한 3-HP 내성 평가 분석방법의 개별적인 점수들은 각각의 균주의 3-HP 내성에 대한 최종 점수를 얻기 위하여 함께 평균을 내었다(표 12). 그 결과, 효모 종 칸디다 아피콜라 (Candida apicola), 칸디다 휴밀리스 (Candida humilis), 아이싸첸키아 오리엔탈리스 (Issatchenkia orientalis), 카자크스타니아 불데리 (Kazachstania bulderi), 카자크스타니아 엑시구아 (Kazachstania exigua), 피치아 멤브라니파시엔스 (Pichia membranifaciens), 사카로마이세스 세레비지애 (Saccharomyces cerevisiae) 및 야로위아 리폴리티카 (Yarrowia lipolytica)가다양한 조건 하에서 낮은 pH에서 3-HP에 대한 일반적 내성이 상당하다는 것을 확인할 수 있었다.
상기 8가지 3-HP 내성 효모 종에 대하여 쉐이크 플라스크 배양액 내 낮은 pH에서 3-HP 내성에 대한 추가 분석을 하였다. 상기 배양액(정제된 SCD-기반 배지, 높은 초기 바이오매스, 낮은 통기)에서, 균주의 성장능, 포도당 소비능 및 에탄올 생성능을 다양한 pH에서 다양한 농도의 3-HP 존재 하에서 평가하였다: 100 g/L 3-HP (pH 4.0), 100 g/L 3-HP (pH 3.5), 100 g/L 3-HP (pH 3.0) 및 80 g/L 3-HP (pH 2.6). 상기 쉐이크 플라스크 배양액은 특정한 C. humilis, K. bulderi, K. exigua 및 S. cerevisaie 효모가 상기 가혹한 조건 하에서 다양한 효모 균주들 중에서 가장 빠른 포도당 이용률, 바이오매스 생성률 및 에탄올 생성률을 나타냈다. 이 결과는 낮은 pH에서 고농도의 3-HP에 대한 매우 탄탄한 내성을 가진다는 점을 나타낸다. 반면에, C. apicola, I. orientalis, P. membranifaciens 및 Y. lipolytica 효모 균주는 이러한 매우 제한적인 성장 조건 하에서 성능을 잘 발휘하지 못하였다. 다양한 쉐이크 플라스크 배양액을 이용한 구체적인 후속적 분석으로 특정한 C. humilis, K. bulderi, K. exigua 및 S. cerevisia 효모 균주가 산업적으로 적합한 조건 하에서 3-HP에 높은 자연적인 내성을 나타내는바, 3-HP 생성 모체로써 큰 잠재성을 가짐을 알 수 있었다.
[표 12] 효모 균주의 3-HP 내성
Figure pat00015

Figure pat00016
Figure pat00017

Figure pat00018
Figure pat00019
Figure pat00020
Figure pat00021
Figure pat00022
Figure pat00023
Figure pat00024
Figure pat00025
Figure pat00026
Figure pat00027
Figure pat00028
Figure pat00029
Figure pat00030
Figure pat00031
Figure pat00032
Figure pat00033
Figure pat00034
Figure pat00035
Figure pat00036

실시예 2: 경로 효소 후보에 대한 생물정보학적 게놈 마이닝(genome mining)
특정한 기능을 가지는 후보 효소를 확인하기 위하여, 상동성-기반 데이터베이스 조사를 수행하였다. 각각 많은 탐색 서열(query sequences)로 데이터베이스를 조사하였다. 추가로, 관련있는 단백질 족(family)를 InterPro 도메인 주석을 기반으로 한 Uniprot/SwissProt으로부터 조사하였다. 상동성-기반 조사는 Uniprot (SwissProt 및 TrEMBL) 및 blastp를 이용한 GenBank 단백질 데이터베이스, tblastn을 이용한 GenBank 뉴클레오티드 데이터베이스(tsa_nt, eny_nt 및 pat)로 수행하였다. E-값(E-value)가 1e-30 보다 작은 서열을 뽑아냈다; 그러나, 몇 가지 경우에 있어서는, E-값이 1e-80 보다 작은 서열을 대상으로 추가적인 분석을 수행하였다. 번역에 대한 안내로써 탐색 서열을 이용한 GeneWise로 뉴클레오티드 히트를 단백질 서열로 번역하였다. GeneWise로 긴 ACC 서열을 번역하는 것이 너무 어려워, 대신에 blast-xml output로부터 단백질 서열(탐색 서열과 일치하는 부분)을 뽑아냈다. 불필요한 서열을 제거하기 위하여, 조사된 서열을 BLASTCLUST 또는 CD-HIT을 이용하여, 각각 상동성이 80% 이상인 서열을 포함하는 클러스터로 집합화하였다. 각각의 클러스터에서 오로지 하나의 대표 서열만을 보존하였다. 서열들의 필요한 세트를 단백질 족의 PFAM 도메인 또는 MAFFT를 이용하여 정렬하였다. MAFFT에 의한 전역 정렬(alignment)은 단백질 족이 어떠한 PFAM과도 관련이 없거나, 단백질의 서열이 수개의 PFAM 도메인으로 분리되는 경우에 적용하였다. 계통수는 PHYLIP 또는 FASTTREE를 이용한 다양한 서열 정렬을 기반으로 만들었다. 계통수는 E.C. 숫자, 개체명, blast E-값으로 주석을 달고, Geneious 소프트웨어를 이용하여 가시화하였다.
2-1: 아세틸기를 함유한 아세트알데히드 디하이드로제나제 (Acetylating acetaldehyde dehydrogenase)
아세트알데히드의 아세틸-CoA로의 환원은 아세틸기를 함유한 아세트알데히드 디하이드로제나제(AADH, E.C. 1.2.1.10)에 의해 행해진다. AADH는 기능적으로 상동성이 있는 세 개의 그룹으로 나뉘어진다(Wei et al.,Nat. Commun., 4:2580, 2013): 1) AADH 및 알코올 디하이드로제나제 활성을 가지는 2기능 단백질 (E. coliadhE type genes, GenBank No: NP_415757, query sequence); 2) 에탄올아민 이화작용에 관여하는 단백질 (E. colieutE type genes, GenBank No: AAG57564, query sequence); 및 3) 4-하이드록시-2-케토발레르산염 (4-hydroxy-2-ketovalerate) 이화작용에 관여하는 알돌레이즈-디하이드로제나제 복합체의 일부인 2기능 단백질 (E. coli mphF type genes, GenBank No: NP_414885). 관심대상은 그룹 1 (adhE type)과 그룹 2 (eutE type) 효소들이다.
adhE 단백질의 N-말단 도메인은 알데하이드: NAD+ 옥시도리덕타아제와 높은 상동성이 있는 반면, C-말단 부위는 Fe2+-의존성 에탄올:NAD+ 옥시도리덕타아제와 상동성이 있다 (Membrillo-Hernandez et al., J. Biol. Chem., 275:33869-33875, 2000). 아세틸기를 함유한 아세트알데히드 디하이드로제나제 활성은 알코올 디하이드로제나제 도메인을 제거함으로써 오로지 N-말단의 알데하이드 리덕타아제 도메인만을 가지는 2기능 adhE 유전자를 절단하여 세포 내로 도입할 수 있다. 이러한 2기능 AADH 활성을 가지는 추가적인 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한 AADH-인코딩 유전자들은 표 1과 같다.
대부분의 장내세균은 탄소원 및 질소원으로써 에탄올아민을 이용할 수 있다(Stojiljkovic et al.,J. Bacteriol., 177:1357-1366, 1995). 이러한 이화경로는 아세트알데히드가 아세틸기를 함유한 아세트알데히드 디하이드로제나제, EutE에 의해 아세틸-CoA로 변환되는 단계와 관련이 있다. AADH 활성을 가지는 신규 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한 AADH-인코딩 유전자들은 표 2와 같다.
추가적으로, adhE 및 eutE 타입 유전자에 대한 생물정보학적 분석 및 서열 상동성을 근거로 하여, AADH 활성을 가지는 것으로 추측할 수 있는 알데히드 디하이드로제나제로 볼 수 있는 하나의 그룹의 유전자를 확인하였다. 본 발명의 구현에 활용 가능한AADH-인코딩 유전자들은 표 3과 같다.
2-2: 진핵 아세틸-CoA 카복실라아제 (Eukaryotic acetyl-CoA carboxylase)
아세틸-CoA 카복실라아제 (ACC, EC 6.4.1.2)는 지방산 생합성에 중요한 효소인 다기능 비오틴-의존성 카복실라아제이다. 아세틸-CoA의 말로닐-CoA로의 전환에 대한 촉매작용을 하기 위하여, 조효소로 ATP 및 비오틴을 사용한다. 우선 비오틴 카복실라아제가 탄산수소염(bicarbonate)과 함께 비오틴의 ATP-의존성 카복시화에 대한 촉매작용을 한다. 두번째로 카복시 트랜스퍼라제가 말로닐-CoA를 형성하기 위하여 비오틴으로부터 아세틸-CoA로 카복시기를 이동시킨다. 진핵 효소들은 큰 멀티도메인 효소들인 반면 이에 상응하는 원핵 효소들은 별개의 유전자에 의해 코딩되는 다양한 서브유닛으로 구성되어 있다. ACC의 활성은 아세틸-CoA 항상성을 유지하기 위하여, 번역 단계 및 번역 후 단계에서 조절된다(예를 들어, 인산화반응 및 응집). 다른 효모 종에서의 ACC 조작은 증가된 ACC 활성 및 증가된 말로닐-CoA 유래 산물의 생성을 유발하였다. ACC 활성을 가지는 효소를 인코딩하는 유전자를 Saccharomyces cerevisiae (GenBank No: CAA96294.1, query sequence), Yarrowia lipolytica (GenBank No: XP_501721.1, query sequence) 및 Mucor circinelloides (GenBank No: EPB82652.1, query sequence)에서 확인하거나 상정하였다. 후보 아세틸-CoA 카복시라아제 유전자들을 Kazachstania exigua (서열번호 1) 및 Candida humilis(서열번호 2)의 새롭게 서열화된 지놈에서 확인하였고, 효모 발현 플라스미드로 클로닝하였다. ACC 활성을 가지는 추가적인 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한 진핵 멀티도메인 ACC-인코딩 유전자들은 표 4와 같다.
2-3: 2기능 말로닐-CoA 리덕타아제 (Bifunctional malonyl-CoA reductase)
말로닐-CoA에서 3-HP로의 환원(말로네이트 세미알데하이드 중간체를 거침)은 C-말단 알데하이드 디하이드로제나제 도메인 및 N-말단 알코올 디하이드로제나제 도메인 기능기를 모두 가지는 큰 2기능 말로닐-CoA 리덕타아제에 의해 행해질 수 있다. 이러한 활성을 가지는 매우 기질-특이적이고 NADPH-의존적인 효소들은 3-하이드록시프로피오네이트 사이클이라고 불리는 독립영양 CO2 고정화 경로에 관여하는 광영양 녹색 비유황세균 Chloroflexus aurantiacus (GenBank No: AAS20429.1; query sequence) 내에서 특성화된다(Hugler et al.,J. Bacteriol.184:2404-2410, 2002). 이러한 2기능 말로닐-CoA 리덕타아제 활성을 가지는 추가적인 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한2기능성 MCR-인코딩 유전자들은 표 5와 같다.
2-4: 말로닐-CoA 리덕타아제 (Malonyl-CoA reductase)
실시예 2-3의 2기능 말로닐-CoA 리덕타아제와 달리, 말로닐-CoA는 두 가지 개별적인 효소에 의하여 3-HP로 전환될 수 있다. 이 경로에 따르면, 말로닐-CoA가 우선 말로닐-CoA 리덕타아제 (MCR; EC 1.2.1.75) 또는 CoA-아실기를 함유한 말로네이트 세미알데히드 디하이드로제나제에 의해 말로네이트 세미알데히드로 환원된 다음, 연속적으로 3-하이드록시프로피오네이트 디하이드로제나제 (3-HPDH; EC 1.1.1.59 또는 EC 1.1.1.298)에 의해 3-HP로 환원된다. MCR은 3-하이드록시프로피오네이트/4-하이드록시부티레이트 사이클을 통해 유기 물질 내로 탄소를 자가영양으로 고정하는 몇몇 내열호산성 고세균(thermoacidophilic archaea)에 의해 이용되는 NADPH-의존성 효소이다(Berg et al., Science, 318:1782-1786, 2007). MCR 활성을 가지는 효소를 인코딩하는 유전자들은 Metallosphaera sedula (GenBank No: ABP94884.1, query sequence) 및 Sulfolobus tokodaii (GenBank No: BAB67276.1, query sequence) 내에서 특성화된다. 비록 상기 MCR들은 Chloroflexus aurantiacus2기능 말로닐-CoA 리덕타아제 효소에 대한 유사한 알데히드 디하이드로제나제 활성을 공유한다고 하더라도, 이들은 Chloroflexus 및 Sulfolobaceae 내 자가영양 경로가 수렴적으로 진화한다는 점 및 다른 유전자들이 분류학상 군 내 유사한 대사과정을 실행하기 위하여 이용된다는 점을 나타내는 어떠한 상당한 서열 유사성을 나타내지 않는다(Alber et al., J. Bacteriol., 188:8551-8559, 2006). 특히, 고세균 MCR은 아스파테이트-세미알데히드 디하이드로제나제와 높은 서열 유사성을 나타낸다. MCR 활성을 가지는 추가적인 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한 단일기능성 MCR-인코딩 유전자들은 표 6과 같다.
2-5: 3-하이드록시프로피오네이트 디하이드로제나제 (3-Hydroxypropionate dehydrogenase)
말로네이트 세미알데히드는 가역 3-하이드록시프로피오네이트 디하이드로제나제(HPDH; EC 1.1.1.59, NADH-의존적) 또는 말로네이트 세미알데히드 리덕타아제 (EC 1.1.1.298, NADPH-의존적)에 의해 3-HP로 환원될 수 있다. 이러한 효소들은 자연적으로 박테리아 및 식물 내 베타-알라닌 대사, 프로파노에이트 대사 또는 우라실 분해에 관여한다. 게다가, 이러한 효소들은 3-하이드록시프로피오네이트/4-하이드록시부티레이트 사이클을 통한 탄소 고정화를 위하여 몇몇 내열호산성 고세균에서 필요로 한다(Kockelkorn and Fuchs, J. Bacteriol., 191:6352-6362, 2009). 3-하이드록시프로피오네이트 디하이드로제나제 또는 말로네이트 세미알데히드 리덕타아제 활성을 가지는 효소를 인코딩하는 유전자를 Escherichia coli (GenBank No: EFV00080.1, query sequence), Saccharomyces cerevisiae (GenBank No: DAA10125.1, query sequence), Metallosphaera sedula (GenBank No: ABP96133.1, query sequence), Sulfolobus tokodaii (GenBank No: BAK54608.1, query sequence) 및 Escherichia coli (GenBank No: ACR64730.1, query sequence)에서 확인하거나 상정하였다. 3-하이드록시프로피오네이트 디하이드로제나제 또는 말로네이트 세미알데히드 리덕타아제 활성을 가지는 추가적인 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한 HPDH-인코딩 유전자들은 표 7과 같다.
2-6: 3-하이드록시이소부티레이트 디하이드로제나제 (3-Hydroxyisobutyrate dehydrogenase)
3-하이드록시이소부티레이트 디하이드로제나제 (HIBADH; EC 1.1.1.31)는 발린 및 다른 분기 사슬 아미노산의 대사에 관여하는 중요한 효소이다. HIBADH는 3-하이드록시이소부티레이트의 메틸말로네이트 세미알데히드로의 NADH- 또는 NADPH 의존성 가역적 전환을 촉매한다. 그러나, 광범위한 기질 특이성에 따라, HIBADH는 말로네이트 세미알데히드를 3-HP로 전환하는 것에 의해 3-하이드록시프로피오네이트 디하이드로제나제 활성 또한 나타낸다(Yao et al., Appl. Biochem.Biotechnol. 160:694-703, 2010). HIBADH 활성을 가지는 효소들은 Pseudomonas putida (GenBank No: ADR61938.1, query sequence), Pseudomonas aeruginosa (GenBank No: AAG06957.1, query sequence), Bacillus cereus (GenBank No: AAP10961.1, query sequence) 및 Alcaligenes faecalis (GenBank No: EJC65559.1, query sequence)내에서 확인하였다. HIBADH 활성을 가지는 추가적인 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한 HIBADH-인코딩 유전자들은 표 8과 같다.
2-7: 4-하이드록시부티레이트 디하이드로제나제 (4-Hydroxybutyrate dehydrogenase)
4-하이드록시부티레이트 디하이드로제나제 (HBDH; EC 1.1.1.61)는 부타노에니트 대사에 자연적으로 관여하는 효소이다. HBDH는 4-하이드록시부타노에이트의 숙시네이트 세미알데히드로의 NAD+-의존적 가역 전환을 촉매한다. 그러나, HBDH는 효소 반응이 유사하기 때문에, 말로네이트 세미알데히드를 3-HP로 전환시킬 수도 있다. HBDH 활성을 가지는 효소들을 Cupriavidus necator (GenBank No: AAC41425.1, query sequence) 및 Clostridium kluyveri (GenBank No: EDK35022.1, query sequence) 내에서 확인하였다. HBDH 활성을 가지는 추가적인 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한 HBDH-인코딩 유전자들은 표 9와 같다.
2-8: 3-하이드록시부티레이트 디하이드로제나제 (3-Hydroxybutyrate dehydrogenase)
3-하이드록시부티레이트 디하이드로제나제 (BDH; EC 1.1.1.30)는 부타노에이트 대사에 자연적으로 관여하는 효소이다. BDH는 3-하이드록시부티레이트의 아세토아세테이트로의 NAD+-의존적 가역 전환을 촉매하나, 다른 3-하이드록시모노카복시산 또한 산화시킬 수 있다. 예를 들어, BDH는 효소 반응이 유사하기 때문에 말로네이트 세미알데히드를 3-HP로 전환시킬 수 있다. BDH 활성을 가지는 효소를 Pseudomonas aeruginosa (GenBank No: GAA17557.1, query sequence) 내에서 확인하였다. BDH 활성을 가지는 추가적인 유전자들을 생물정보학적 분석 및 서열 상동성을 기반으로 추론해냈다. 본 발명의 구현에 활용 가능한 BDH-인코딩 유전자들은 표 10과 같다.
실시예 3: 효소 역가 측정
ACC 분광광도 효소 어세이 ( ACC spectrophotometric enzyme assays )
분광광도 ACC 어세이는 커플링된 어세이로, 보조인자인 NAD(P)H가 필요한 반응에서 ACC 반응에 의해 생성된 산물이 추가로 소비되고, 이의 산화를 분광광도계로 모니터링할 수 있다.
Kroeger et al. (2011, Anal. Biochem. 411:100-105)는 ACC1에 의해 생성된 malonyl-CoA가 NADPH가 보조인자로 요구되는 반응에서, 정제된 malonyl-CoA reductase (MCR)에 의해 말로네이트 세미알데하이드로 더 변환되는 커플링된 어세이를 기재하고 있다.
Diacovich et al. (2002, J. Biol. Chem. 277:31228-31236) 에서 ADP와 ACC 반응에서 보조인자로 사용되는 ATP 가수분해 산물을 pyruvate kinase 반응을 필요로 하는 ADP (ADP-requiring pyruvate kinase reaction)로 전환하는 것과 lactate dehydrogenase를 사용하여 피루베이트를 형성하는 것과 추가로 커플링하여 조합시켰다. 후자는 NADH를 보조인자로 필요로 하고, 이의 산화가 연속된다.
ACC 방사성 효소 어세이 (ACC radioactive enzyme assays)
in vitro ACC 어세이에서 가장 흔히 사용되는 것은 방사성 14C carbonate의 사용에 기초한 것이다. 방사성 carbonate를 산 및 비휘발성 물질 (즉, malonyl-CoA)에 도입하는 과정이 이어진다. malonyl-CoA로 전환되지 않은 14C로 라벨링된 sodium bicarbonate을 산 및 열처리로 제거하고, 나머지 NaH14CO3 및 가능한 반응 부산물을 14C-라벨링된 CO2로 전환한다.
Diacovich et al. (2002, J. Biol. Chem. 277:31228-31236)에 기재된 이 어세이는 약간 변형되어 효모 용해물에서 ACC 활성을 검출하는 데 사용되어 왔다 (Shi et al. 2014, mBIO 5:3 e01130-14). Late exponential 또는 stationary phase 중 하베스트한 효모 세포들에서 세포용해물을 준비하였다. 세포를 수세하고, 100 mM 포타슘 포스페이트, pH 7.5, 2 mM MgCl2, 1 mM dithiothreitol 및 1x EDTA free Complete protease inhibitor (Roche)를 포함한 용해 버퍼에 다시 서스펜션하였다. 세포를 글라스 비드로 파쇄하고, 4℃에서 원심분리한 다음 상층액을 수집하였다.
ACC 효소 어세이 반응 혼합물은 100 mM 포타슘 포스페이트(pH 8.0), 300 μg BSA, 3 mM ATP, 5 mM MgCl2, 10 mM NaH14CO3 [비활성 (pecific activity) 200 μCi mmol-1 (7400 kBq mmol)] 및 0.5 mM acetyl-CoA을 포함한다. 반응 총 부피는 100 ㎕이고, 20 ㎕의 세포 추출물을 포함한다.
반응물을 30℃에서 15분 동안 인큐베이션하고, 50 μL의 5 M HCl을 추가하고 정지하였다. 튜브 내용물을 95℃에서 건조하여 증발시키고, 잔여물을 100 μL의 물에 재서스펜션한 다음 3 mL의 scintillation cocktail (Ultima Gold AB, PerkinElmer)와 혼합하였다. liquid scintillation counter (PerkinElmer Tri-Carb 2810TR)를 사용하여 샘플의 14C 함량을 측정하였다.
AADH 효소 어세이
Kozak et al. (2014, Metab. Eng. 21:46-59)에 기재된 바와 같이 30 ℃에서 340 nm NAD+ 환원을 모니터링하여 AADH 활성을 측정하였다. 세포 용해물을 위해 효모 세포를 수집하고, 물로 수세한 다음 100 mM Tris-HCl 버퍼 (pH 7.5) 및 1X EDTA 미포함 프로테아제 저해제 칵테일 (1X EDTA free protease inhibitor cocktail (Roche))을 포함한 용해 버퍼에 다시 서스펜션하였다. 세포를 5500 rpm Precellys 24 homogenizer에서 글라스 비드로 3x40 동안 용해하고, 각 회 사이에 얼음 에서 유지하였다. 용해물은 4 ℃에서 16 000 g로 20분 동안 원심분리하였고, 상층액을 수집하였다. Bradford method를 사용하여 총 단백질 농도를 측정하였다.
효소 어세이 반응 혼합물은 총 반응 부피 200 ㎕에 0.1 mM Coenzyme A, 50 mM CHES buffer (pH 9.5), 0.8 mM NAD+, 0.2 mM DTT 및 10 ㎕ 세포 추출물을 포함하였다. 10 mM의 미반응한 상태로 준비된 아세트알데히드 용액을 추가하여 반응을 시작하였고, Thermo Konelab 20XT analyser를 통해 NAD+의 환원이 연속되었다.
효모 세포 용해물로부터 MCR 효소 어세이
Chen et al. (2014, Metab. Eng. 22:104-109)에 기재된 방법을 약간 변형하여 MCR 효소 활성을 측정하였다. 이 방법은 340 nm에서 NAD(P)H의 산화를 모니터링한 것에 기초한 것이다.
세포를 수집하고, 20 mM Tris-HCl (pH 7.5), 20 mM NaN3를 포함하는 찬 수세 버퍼로 수세한 다음, 50 mM HEPES (pH 7.5), 150 mM KCl, 1 mM DTT, 1 mM EDTA, 0.5 % Triton X-100 및 1X EDTA free protease inhibitor cocktail (Roche)를 포함하는 1 mL breaking buffer에 재서스펜션하였다. 세포를 5500 rpm Precellys 24 homogenizer에서 3x40초 동안 글라스 비드로 용해하고, 각 회 사이에 얼음에서 유지하였다. 용해물을 16 000 g에서 20분 동안 4 ℃에서 원심분리하고, 상층액을 수집하였다. Bradford method를 사용하여 총 단백질 농도를 측정하였다.
MCR 어세이 혼합물은 50 mM Tris-HCl buffer (pH 8.0), 5 mM MgCl2 및 0.3 mM NADPH 또는 NADH를 포함하였다. 20 ㎕ 세포 용해물을 총 반응 부피 200 ㎕에 추가한 다음, 반응물을 5분 동안 30 ℃에서 사전 인큐베이션한 후, 0.15 mM malonyl-CoA를 추가하여 반응을 시작하였다. Thermo Konelab 20XT analyser로 340 nm에서 어세이를 모니터링하였다.
효모 발현 벡터의 제작
후보 유전자들의 발현 및 효모에서 활성능을 평가하기 위하여, 일련의 효모 발현 플라스미드를 제작하였다. 먼저, 신규 pBlueScript 기반 MCS (multiple cloning site)를 디자인하여, 모든 가능한 RE (restriction enzyme) 사이트 조합을 사용할 수 있다. 이후 변형된 MCS를 pRS-기반 일련의 효모 동원체 및 다카피 플라스미드에 위치시켰다. 이후, 10 고유 RE 사이트 세트를 사용하여, 9개의 다른 세트의 프로모터 및 터미네이터를 pRS- 기반 효모 발현 벡터에 클로닝하였다. 이후, 3-HP 생산을 위한 다양한 경로 효소 조합을 평가하기 위해, 이러한 플라스미드 시스템을 사용하여 연속적으로 9개의 유전자를 낮은 또는 높은 카피수로 동시에 적합한 효모 균주에서 발현하였다.
ACC 유전자 클로닝
SNF1 결실이 있거나 없는 산업적으로 이용되는 S. cerevisiae VSK-128 균주에 형질전환시킨 ACC1 유전자를 표 13에 나타내었다. 다카피 플라스미드에서 ACC 유전자를 발현하였으며, 이들은 PDC1 p프로모터의 조절하에 있었다. QuikChange II Site Directed Mutagenesis Kit (Agilent Technologies)를 사용하여 Shi et al. (2014)에 기재된 변형된 ACC1sc 유전자들을 구축하였다.
표 13. S. cerevisiae VSK-128에 형질전환된 Acetyl-CoA carboxylase 유전자들.
Figure pat00037

ACC 효소 어세이
문헌에서는 ACC 효소 활성을 감지하기 위해 정제된 또는 일부 정제된 ACC 효소와 함께 분광광학적 어세이 (spectrophotometric assays)가 사용되어 왔다. 대조군과 비교하여 흡광 변화가 감지되지 않지만 ACC가 과발현된 효모 세포 용해물을 분광광학적 어세이로 테스트하였다.
방사성 ACC 효소 어세이는 매우 민감하여 심지어 pmol/mg/min 활성도 감지할 수 있다. 상업적으로 입수 가능한 정제된 인간 ACC 효소를 통해 먼저 테스트하였다. 충분한 활성이 감지됨에 따라, 효모 세포 용해물에서 ACC 활성을 감지하도록 방법을 더 최적화하였다. 표 13에 리스트된 균주의 ACC1 활성에 대하여 어세이하고, 그 결과에 기초하여 ACC1의 상대적 순위를 기재한 리스트를 제작하였다 (도 2). 가장 유망한 후보로, ACCyl에 대하여 추가로 연구하였으며, S. cerevisiae S-128 야생형 균주 및 ACC1 이 과발현된 동일 균주에 대한 결과를 표 14에 나타내었다.
표 14. 야생형 S. cerevisiae VSK-128 균주의 내인성 ACC1 활성과 비교하여 ACCyl 유전자의 과발현으로 ACC1 효소 활성이 2.9배 증가되었음
Figure pat00038

AADH in vitro 효소 활성 어세이
5개의 AADH 유전자 (즉, ADHEpm, ADHEec, EUTec, EUTdz 및 LIN1129li) 를 우선적으로 선택하고, CEN.PK lab 균주로 형질전환하였다. TEF1 프로모터 조절하에 있는 다카피 플라스미드에서 이러한 AADH가 발현하였다. 모든 5개 AADH 유전자는 AADH 활성을 보였으나, 3개의 eutE-type AADH 유전자 (즉, EUTec, EUTdz 및 LIN1129li)는 adhE-type AADHs (즉, ADHEpm 및 ADHEec)과 비교하여, 효모에서 훨씬 더 높은 AADH 활성을 나타내었다.
20개의 추가 신규 AADH 유전자를 게놈 마이닝 분석으로부터 선택하고, 효모에서 발현 및 효소 활성을 평가하였다. 이러한 20개의 신규 AADH 유전자를 in vitro 효소 활성에 대하여 테스트하였고, 이들 중 4개 (즉, AADHmm, AADHab, AADHbw 및 AADHvs)는 AADH 활성을 가진 것으로 나타났다.
AADH in vivo 성장 어세이
글루코스 기반 배지에서 성장하지 못하도록 PDH-bypass가 결여된 균주를 제작하고자, 산업적 S. cerevisiae VSK-128 균주에서 모든 카피의 ACS2 유전자를 결실시켰다. 25개의 상이한 AADH 변이체를 포함하는 발현벡터를 이러한 ACS2 결실 균주에 형질전환하여, 글루코스 상에서 이러한 균주의 성장 회복능을 평가하였다.
12개의 다른 AADH 변이체 (즉, EUTEec, EUTEdz, LIN1129li, AADHmm, AADHtl, AADHab, AADHta, AADHbs, AADHbw, AADHvs, AADHhs 및 ADHEec)는 글루코스 기반 아가 플레이트에서 성장능에 기반하여, ACS2-결실 균주 성장 결핍을 회복할 수 있었다. in vitro AADH 효소 활성 어세이 및 in vivo 성장 회복 분석 사이에 우수한 상관성이 있었다.
ACS2 결실 균주의 성장을 회복할 수 있는 것으로 보이는 첫번째 9개의 AADH 변이체 균주의 성장률을 액상 쉐이크 플라스크 배양 (liquid shake flask cultivations)에서 평가하였고, 변형되지 않은 PDH-bypass를 포함하는 야생형 균주와 비교하였다. 이러한 9개의 AADH 변이체 모두는 글루코스에서 배양되었을 때 S. cerevisiae VSK-128 균주의 호기성 성장율에 대하여 >50%를 유지할 수 있었고, AADH 변이체 중 6개는 S. cerevisiae VSK-128 균주의 호기성 성장율에 대하여 ≥80%를 유지할 수 있었다.
효모에서 MCR 효소 어세이
8개의 전장 Chloroflexus MCR 호모로그를 2개의 기능성 도메인으로 절단하고, 효모에서 6개 아키아 MCR과 함께 효소 활성에 대하여 MCR-특이적 도메인을 어세이하였다. 이러한 MCR은 다카피 플라스미드에서 발현되었고, 이들은 TEF1 프로모터 하에서 조절되었고, CEN.PK lab 균주에 형질전환되었다.
MCRca (Chloroflexus aurantiacus) 및 이의 호몰로그 MCRrc (Roseiflexus castenholzii)는 NADPH를 보조인자로 사용하였을 때 야생형 균주보다 더 높은 MCR 효소 활성을 보이는 유일한 2개의 Chloroflexus MCR 호몰로그이다. NADH를 보조인자로 사용하였을 때는 8개의 Chloroflexus MCR 호몰로그에서 어떠한 효소 활성도 관찰되지 않았다.
3개의 아키아 MCR [Sulfolobales archaeon, Sulfolobus acidocaldarius (x2)]은 NADPH를 보조인자로 사용하였을 때 야생형 균주보다 더 높은 MCR 효소 활성을 나타내었고, 모든 6개의 아키아 MCR은 NADH를 보조인자로 사용하였을 때 야생형 균주보다 더 높은 MCR 효소 활성을 나타내었다.
아키아 MCR의 이종 발현 및 특성
4개의 상이한 아키아 MCR Metallosphaera sedula (MCRms), Sulfolobus tokodai (MCRst), Candidatus caldiarchaeum (MCRcc), 및 Sulfolobales archaeon (MCRsa1)에 대하여 pBAT T7 프로모터 기반 발현 구조물을 제작하였다. 이러한 구조물은 정제 태그를 포함하지 않으며, E. coli 코돈 최적화되었고, 앞서 기재한 조건에서 발현되었다 (표 15).
표 15. E. coli에서 발현하기 위한 아키아 MCR 구조물의 개요 (ca - Chloroflexus aurantiacus, ms - Metallosphaera sedula, st - Sulfolobus tokodaii, cc - Candidatus caldiarchaeum, sa1- Sulfolobales archaeon)
Figure pat00039

다음의 어세이 조건을 사용하여 활성을 분석하였다: 0.4 mM NAD(P)H; 0.15 mM Malonyl-CoA; Tris-HCl pH 7; 2 mM MgCl2.. 20배 희석된 용해물에 대하여 실온에서 MTP (microtitre plate) 포맷으로 어세이를 수행하였다. 이후 A365에서 NADPH 또는 NADH의 산화가 연속되었다. 테스트한 4개의 아키아 유전자 중에서, MCRsa1 및 MCRst가 가장 높은 MCR 활성을 나타내었다. 그러나, 태깅된 MCRca에 대하여 측정된 것보다 MCR 활성이 더 적었다. Candidatus caldiarchaeum MCR (MCRcc)에 대하여 (E.coli 세포 용해물 중) NADPH 또는 NADH에 대한 활성은 측정되지 않았다.
SDS-PAGE 겔을 사용하여 이러한 구조물을 분석하는 경우, E.coli 용해물에서 MCRsa1가 가장 높은 발현 수준을 보이는 반면, MCRcc는 용해된 형태로 발현되지 않았다: 도 3의 겔 (sa1 > ca > st > ms > cc) 참조. 구조물 MCRsa1 및 MCRst은 이중 보조인자 우호적인 것처럼 보이고, NADPH에 대하여 측정한 것과 비교하였을 때 약 40-50% 상대적 NADH 활성을 나타내었다. 비활성 (specific activity) 측면에서, MCRst는 테스트한 4개의 아키아 MCR 중 가장 활성있는 효소일 수 있는데, 이는 상대적으로 가장 낮은 발현 수준에서 상대적으로 가장 높은 활성 수준을 나타내었기 때문이다.
실시예 4: 재조합 효모 배양을 통한 3-HP의 제조
S. cerevisiae 에 대한 쉐이크 플라스크 배양 (Shake flask cultivations)
선별적 아가 기반 플레이트에서 갓 배양된 균주로부터 작은 고리의 세포를 취하여 250 mL 플라스크에서 20 mL의 선별적 SC 기반 배지를 접종하는 데 사용하고, 모든 글루코스 및 에탄올이 소비될 때까지 2일 동안 (30 oC, 250 rpm) 배양하였다. 최종 세포 밀도를 측정하고, 배양물을 5분 동안 4000 rpm으로 원심분리하였다. 상층액을 HPLC 또는 GC/MS로 분석하여, 배양 상층액에서 3-HP 및 기타 주요 대사물의 축적을 측정하였다. 그러나, 다른 배양 조건 또한 특정 균주, 목적 (예를 들어, 글루코스 시작량, 공기의 양, 배지의 종류 및 배지에 추가물질의 추가 등)에 따라서 테스트하였다.
S. cerevisiae 바이오리액터 배양
250~500 mL 배지를 포함하는 Multifors 바이오리액터 (최대 작동 부피 500 mL, 2 4-블레이드의 Rusthon 터빈 임펠러, Infors HT, Switzerland)에서 배양하였다. 배양물을 초기 1.2, 2.4 또는 3.6 볼륨 가스 (부피 배양)-1 min-1 (vvm)로 30℃, 300 또는 900-950 rpm으로 유지하였다. 배양물 pH는 2M NaOH 또는 2M H3PO4를 추가하여 pH 5.5 ± 0.2로 일정하게 유지되었다. Clerol FBA 3107 거품방지제 (Cognis France, Ponthierry Paris; 0.03% v/v)를 추가하여 거품 생성을 조절하였다. Ar 중 3% CO2, 5% CO2와 0.99% Ar 및 N2 중 15% O2, N2 중 20% O2 플러스 20% Ar 및 N2 중 0.04% 에탄올로 조정된 Prima Pro Process mass spectrometer로 가스 농도 (CO2, O2, N2 및 Ar)를 지속적으로 분석하였다.
균주를 쉐이크 플라스크에서 SCD 기반 선별 배지 중 오버나이트로 사전 배양하였고, 바이오리액터에 접종하는데 사용하였다. 배양물의 배치 단계 (20 g/L 초기 글루코스)를 허용하여 14-20 시간 동안 지속하였고, 글루코스가 소비된 이후에만 다만, 에탄올이 소비된 이후 또는 이전 둘 중 어느 하나 (배양 목적에 따라)에 글루코스 피드를 시작하였다. 글루코스 피드율은 0.38-0.65 g L-1 h-1 (배양 목적에 따라)로 유지하였다. 이후 상층액 샘플을 HPLC로 분석하여 배양물 중 3-HP와 기타 주요 대사물의 축적을 측정하였다.
HPLC에 의한 세포 배양 상층액에서 3-HP 분석
주입 부피가 10 μl인 Waters Alliance e2695 HPLC system (Waters, Milford, USA)을 사용하여 배양 상층액 샘플을 분석하였다. HPLC에서 Fast Acid Analysis Column (100 mm X 7.8 mm) (Bio-Rad, USA)에 연결된 Aminex HPX-87H Organic Acid Column (300 mm X 7.8 mm) (Bio-Rad, USA)을 정체기로 사용하였다. 컬럼을 +55 ℃로 유지하고, 0.3 또는 0.5 ml min-1의 유량으로 5.0 mM H2SO4 (Merck KgaA, Germany)를 용출액으로 사용하였다.
3-hydroxypropionic acid, 글루코스, 아세테이트, 숙시네이트, 피루베이트, 글리세롤 및 에탄올의 검출에 Waters 2489 dual wavelength UV (210 nm) detector (Waters, Milford, USA) 및 Waters 2414 differential refractometer (Waters, Milford, USA)을 사용하였다.
GC/MS를 이용하여 세포 배양 상층액으로부터 3-HP 분석
테스트 샘플 및 표준 커브는 다음의 방법으로 준비하였다: 50 μl HCl (6N)로 상층액 (0.5 ml)을 산성화하고, 3-HPA (TCI) 표준액으로 스파이크하였다. 5 μl 젖산 내부 표준 용액 (Sigma Aldrich (ISOTEC) sodium L-lactate-3,3,3-d3 98 atom %; 5.5 g/l) 및 약 0.2 g의 NaCl을 추가하였다. 라벨링된 3-HP는 상업적으로 입수 불가능하므로, 이러한 젖산 안정한 동위원소 제품을 내부 표준으로 선택하였고, 샘플 매트릭스에 존재하지 않는 것으로 이용가능하면서도, 3-HP와 구조적으로/화학적으로 가장 유사한 화합물이기 때문이다. 혼합물을 약 3분 동안 볼텍스 믹서에서 쉐이킹하였다. 이후 샘플을 약 3분 동안 볼텍스 믹서에서 혼합하여 에틸 아세테이트 0.5 ml로 2회 추출하였다. 10 000 rpm에서 5분 동안 원심분리하여 층을 분리하였다. GC 바이알로 상층을 수집하고 증발시켰다. 건조된 잔여물은 60 ℃에서 1시간 동안 인큐베이션하여 1% TMCS 포함 MSTFA (50 ㎕)로 유도하였다. 오차를 최소화하기 위하여 샘플과 동일한 방법으로 검정 커브 (calibration curve)에 대한 표준을 추출하였다.
MSD (Agilent 5973 mass selective detector)가 장착된 Agilent 6890 gas chromatograph (GC)에 샘플을 흘렸다. 주입기 (주입 부피 1 ㎕이고, 분할비 (split ratio) 20:1) 및 MSD 인터페이스 온도는 280℃이었고, 오븐 온도 프로그램은 20℃/min의 속도로 50℃ 내지 280 ℃이었다. Agilent HP-5MS capillary column (30 m, ID 200 μm, 필름 두께 0.25 μm; Agilent 19091S-433) 상에서 분석을 수행하였다. 화합물의 확인은 NIST library에서 스펙트럼 서치에 기반하였다. m/z 147과 m/z 219을 모니터링하여 3-HP를 확인하였고, m/z 177를 모니터링하여 3-HP dimer를 검출하였다. 3-HP 반응을 사용하여 5점 검정 커브 (c=1-400mg/l)룰 구축하고, 정규화를 위해 내부 표준을 사용하였다. 이러한 농도 범위에서 정량은 선형인 것으로 입증되었다. 샘플들과 함께 공백 샘플을 분석하였다.
S. cerevisiae 플라스미드 발현 균주의 평가
3-HP 플라스미드 발현 균주는 2개의 다른 발현 플라스미드 (즉, pSK-084 및/또는 pSK-085)로부터 3-HP 경로 효소 (즉, AADH, ACC1, MCR 및 HPDH) 하나, 둘 또는 3가지를 동시에 발현하였다 (도 4). 이러한 균주를 사용하여 VSK-128 산 저항성 S. cerevisiae 균주에서 3-HP 생산에 대한 다른 3-HP 경로 효소 (및 이들 효소의 조합)의 효과를 평가하였다. 균주를 250 mL 플라스크 중 20 mL의 선별 SC 기반 배지 (20 g/L 글루코스)에서 배양하고, 모든 글루코스 및 에탄올이 소비될 때까지 2일 동안 (30oC, 250 rpm)에서 배양하였다.
in vivo 경로 효소 활성 분석 요약
필요하다면 추가의 3-HP 경로 효소를 발현하기도 하는 다양한 S. cerevisiae 균주에서 표 16에서와 같이 25개의 AADH, 8개의 ACC1, 10개의 2기능성 HPDH-MCR (bifunctional HPDH-MCR), 6개의 아키아 MCR 및 28개 HPDH의 3-HP 생산능에 대하여 분석하였다. 많은 신규 3-HP 경로 효소 (게놈 마이닝 분석을 통해 수득)가 효소에서 이전 공개된 3-HP 경로 효소와 비교하여 활성이 있음을 나타내고, 우수한 특성 (즉, 더 높은 활성, 더 우수한 보조인자 선호도)을 보유함을 나타내었다.
[표 16]
Figure pat00040
Figure pat00041
Figure pat00042

3-HP 생산을 위한 S. cerevisiae 쉐이크 플라스크 배양 시도
배양 조건
초기 테스트한 균주에 대하여 다양한 다른 배양 조건을 평가함으로써, 다양한 배양 조건이 균주의 3-HP 생산능에 영향을 미치는지 확인하였다. S. cerevisiae VSK-128 (Δura3, Δhis3) 균주는 2개의 플라스미드를 발현하는데, 하나는 HIBADHpa 또는 HPDHec 유전자를 포함하고, 2번째 플라스미드는 MCRsa2 유전자를 포함한다. 2개의 균주는 배양하는 동안 유사하게 행동하고, 매우 유사한 대사물 프로필을 가진다.
6개의 다른 쉐이크 플라스크 배양 조건을 시험하였다:
1. 호기성, 회분식 (batch) 공정, 초기 고농도 글루코스 (120 g/L). 3일째 추가 글루코스 100 g/L 첨가하였다.
2. 혐기성, 회분식 공정, 초기 고농도 글루코스 (100 g/L). 플라스크를 밀봉하고, 100 rpms으로 더 천천히 쉐이킹하였다.
3. 호기성, 회분식 공정, 반복적 글루코스 스파이킹. 초기 글루코스 20 g/L, 이후 매일 40 g/L을 첨가하였다.
4. 호기성, 가상의 유가식 (fed-batch) 공정, 초기 다수의 글루코스 정제 (glucose tablets). 5 개의 정제를 초기에 첨가하고, 추가 5개 정제를 3일째에 첨가하였다. 다양한 양의 Enzyme A 용액 (50-150 ㎕ per day) 을 매일 첨가하였다.
5. 호기성, 가상의 유가식 공정, 반복적 글루코스 정제 스파이킹. 1 개의 정제를 초기에 첨가하고, 2 개의 정제를 1일 및 2일에 첨가하고, 3 개의 정제를 3일 및 4일에 첨가하였다. 다양한 양의 Enzyme A 용액 (50-150 ㎕ per day) 을 매일 첨가하였다.
6. 호기성, 회분식 공정, 반복적 갈락토오스 스파이킹. 초기 갈락토오스는 20 g/L이었고, 이후 매일 40 g/L 첨가하였다.
가상의 유가식 공정에 대하여, 각 정제는 0.5 g의 글루코스 (25-mL 배양 부피에 대하여 20 g/L의 글루코스에 대응됨)를 방출하는 것으로 고려된다. 정제는 필수적으로 글루코스 (즉, 전분) 및 Enzyme A 용액 (아밀라제)로 구성되어 있어, 쉐이크 플라스크 배양 동안 배지로 글루코스가 조절되어 느리게 방출되도록 한다. 글루코스가 제한된 fed-batch 조건에 의해 성장으로의 플럭스가 증진되어 이에 따라 3-HP 생산이 증진되는 것으로 고려된다.
세포 성장
모든 일반적인 글루코스 기반 배양에 의해 유사하게 배양되고, 갈락토스 유가배양에 의해 더 느리게 배양된다. 한편, 다른 배양 조건과 비교하여, 유가배양 조건에 의해 세포 성장이 더욱 증진된다. 특히, 유가배양 (정제 스파이킹) 조건은 초기 배양에서 성장을 상당히 증진시킨다.
3-HP 생산
이러한 배양을 통해, 대부분의 성장은 일반적으로 2일 후반에 일어나고, 상당히 대부분의 3-HP 또한 2일째 생산되었으며, 이는 성장과 3-HP 생산은 서로 연관성이 있음을 시사한다. 유가배양 (정제 스파이킹) 배양 조건에서 3-HP (~0.85 g/L)가 가장 많이 생산되었고, 갈락토스 유가배양을 통해 가장 적은 양의 3-HP (~0.12 g/L)가 생산되었으며, 다른 배양 조건에서 모두 약 0.3 내지 0.4 g/L의 3-HP를 생산하였다. 이러한 결과는 배양 조건에 3-HP 생산 수준에 큰 영향을 미칠 수 있다 (도 5).
글루코스 소비
모든 배양에서 2-3일까지 글루코스가 빠르게 소비되었고, 이후 글루코스 소비율은 성장 및 3-HP 생산과 함께 현저히 감소하였다 (4-5일째). 유가배양에서, 글루코스는 정제에서 방출되는 만큼 빠르게 소비되는 것처럼 보이는데, 이는 이러한 배양이 글루코스 제한적 조건하에서 수행되었음을 제시한다.
글리세롤, 아세테이트 및 에탄올 축적
글루코스 유가배양에서 글리세롤 축적은 꽤 높고, 갈락토스 유가배양보다 훨씬 낮다. 아세테이트 축적은 다른 배양 조건 사이에서 상당히 유사하나, 유가배양 (다수의 정제) 조건에서 더 많은 아세테이트가 생산되었다.
이러한 쉐이크 플라스크 배양 특히 글루코스 회분식 배양과 글루코스 스파이킹 배양 대부분에서 고농도의 에탄올이 축적되었다. 유가배양은 글루코스 제한된 농도를 대표하여 에탄올로의 과량의 과유량 대사물을 감소시키고, 이러한 접근은 에탄올 축적이 유가배양에서 상당히 낮기 때문에 성공한 것처럼 보인다.
추가로 정립된 3-HP 생산 균주 배양
추가의 3-HP 생산 균주 [(EutEec, HPDH-MCRca, ACC1sc_m2) 및 (HIBADHpa, MCRsa2)]를 가장 유망한 유가배양 (정제 스파이킹) 배양 조건에 따라 배양하여 성능을 확인하였다.
대부분의 성장은 3일째에 일어났고, 대부분의 3-HP 생산도 3일째에 일어났다. 이러한 배양 조건하에서는 이러한 균주에서 3-HP 생산이 1.2 g/L를 초과하였다 (도 6).
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적 기술은 단지 바람직한 실시태양일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.
<110> SK INNOVATION CO., LTD. <120> Recombinant Yeast Producing 3-Hydroxypropionic Acidand Method for Producing 3-Hydroxypropionic Acid Using the Same <130> 1614 <150> KR 14/114,505 <151> 2014-08-29 <160> 144 <170> KopatentIn 2.0 <210> 1 <211> 2248 <212> PRT <213> Kazachstania exigua <400> 1 Met Ser Glu Glu Asn Leu Phe Glu Asp Ser Ser Ala Lys Arg Gln Tyr 1 5 10 15 Glu Ile Thr Asp Tyr Ser Lys Lys His Lys Ala Leu Ala Pro His Phe 20 25 30 Val Gly Leu Asn Thr Leu Asp Lys Val Glu Glu Ser Pro Leu Lys Asp 35 40 45 Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile 50 55 60 Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys 65 70 75 80 Trp Ser Tyr Glu Thr Phe Gly Asp Glu Arg Thr Val Gln Phe Val Ala 85 90 95 Met Ala Thr Pro Glu Asp Leu Gln Ala Asn Ser Glu Tyr Ile Arg Met 100 105 110 Ala Asp Gln Tyr Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr 115 120 125 Ala Asn Val Asp Leu Ile Val Asp Val Ala Glu Arg Thr Asp Val Asp 130 135 140 Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro His Leu Pro 145 150 155 160 Glu Lys Leu Ala Ala Ser Lys Arg Lys Ile Ile Phe Ile Gly Pro Pro 165 170 175 Gly Ser Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val 180 185 190 Ala Gln His Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Ile 195 200 205 Asp Lys Val His Val Asp Glu Thr Ser Gly Leu Val Ser Val Asp Asp 210 215 220 Asp Val Tyr Gln Gln Gly Cys Cys Gln Ser Pro Glu Asp Gly Leu Ala 225 230 235 240 Lys Ala Lys Gln Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly 245 250 255 Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile 260 265 270 Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe 275 280 285 Ile Met Lys Leu Ala Gly Lys Ala Arg His Leu Glu Val Gln Leu Leu 290 295 300 Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser 305 310 315 320 Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile 325 330 335 Ala Asn Asn Glu Thr Phe Asp Lys Met Ala Asn Ala Ala Val Arg Leu 340 345 350 Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr 355 360 365 Ser Asn Glu Glu Lys Lys Tyr Tyr Phe Leu Glu Leu Asn Pro Arg Leu 370 375 380 Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro 385 390 395 400 Ser Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser 405 410 415 Asp Ile Arg Val Leu Tyr Gly Met Asn Pro His Ser Ala Thr Glu Ile 420 425 430 Asp Phe Glu Phe Lys Thr Glu Glu Ala Lys Lys Thr Gln Arg Lys Pro 435 440 445 Val Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro 450 455 460 Asn Glu Gly Phe Lys Pro Ser Gly Gly Ser Leu His Glu Leu Asn Phe 465 470 475 480 Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly 485 490 495 Gly Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe 500 505 510 Gly Glu Asn Arg Gln Met Ser Arg Lys His Met Val Val Ala Leu Lys 515 520 525 Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile 530 535 540 Lys Leu Leu Glu Thr Glu Asp Phe Glu Glu Asn Thr Ile Thr Thr Gly 545 550 555 560 Trp Leu Asp Asp Leu Ile Ser His Lys Met Thr Ala Glu Lys Pro Asp 565 570 575 Ala Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Ile Ala 580 585 590 Ser Glu Ser Ala Arg Met Glu Tyr Ile Ala Gln Leu Lys Arg Gly Gln 595 600 605 Val Pro Ala Lys Asn Leu Leu Glu Thr Met Phe Pro Val Glu Phe Ile 610 615 620 His Glu Asn Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Ala Asp Asp 625 630 635 640 Ser Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Glu Val Arg Ala Val 645 650 655 Lys Leu Ser Asp Gly Gly Leu Leu Ile Ser Val Gly Gly Gln Ser His 660 665 670 Thr Ile Tyr Trp Lys Glu Glu Ala Gln Ala Thr Arg Leu Ser Ile Asp 675 680 685 Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg 690 695 700 Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Asp 705 710 715 720 Gln Ile Lys Val Gly Gln Ala Tyr Ala Glu Ile Glu Val Met Lys Met 725 730 735 Gln Met Pro Leu Val Ser Gln Glu Asn Gly Val Ile Gln Leu Leu Lys 740 745 750 Gln Pro Gly Ser Thr Ile Ala Ala Gly Asp Ile Ile Ala Met Leu Ala 755 760 765 Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met 770 775 780 Leu Pro Glu Leu Gly Ser Pro Met Val Glu Gly Thr Lys Pro Ala Tyr 785 790 795 800 Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr 805 810 815 Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val 820 825 830 Leu Arg Asp Pro Arg Leu Pro Tyr Ser Glu Trp Lys Leu Cys Val Ser 835 840 845 Ala Leu His Ser Arg Leu Pro Met Ser Leu Asp Glu Lys Leu Asp Gln 850 855 860 Leu Val Asp Arg Ser Ser Asn Arg Asn Ala Ile Phe Pro Ala Lys Gln 865 870 875 880 Leu Asn Lys Met Ile Glu Asp Ala Val Arg Glu Pro Gly Ser Asp Glu 885 890 895 Leu Leu Pro Thr Ile Val Glu Pro Leu Leu Asp Ile Thr Asn Arg Tyr 900 905 910 Ala Asn Gly Leu Glu Ala His Glu His Ser Val Phe Val Lys Phe Leu 915 920 925 Glu Glu Tyr Tyr Asn Val Glu Lys Leu Phe Ser Ser His Gly Asn Arg 930 935 940 Glu Glu Asn Val Ile Leu Lys Leu Arg Asp Glu Asn Leu Asp Ser Leu 945 950 955 960 Asp Lys Val Val Glu Ile Val Leu Ser His Ala Lys Val Ser Ala Lys 965 970 975 Asn Thr Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Ile Cys Lys 980 985 990 Met Ser Ser Ser Val Ala His Ile Phe Ser Lys Ser Leu Gly His Ile 995 1000 1005 Val Glu Leu Glu Ser Lys Ser Ala Ala Lys Val Ala Leu Gln Ala Arg 1010 1015 1020 Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg Thr Glu 1025 1030 1035 1040 Gln Ile Glu His Ile Leu Arg Ser Ser Val Thr Asn Thr Ser Tyr Gly 1045 1050 1055 Asp Leu Ala Pro Lys Lys Ser Glu Pro Asn Leu Glu Ile Leu Lys Asp 1060 1065 1070 Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val Leu Leu Pro Phe Leu 1075 1080 1085 Cys His Glu Asp Pro Val Ile Ala Thr Ala Ala Ala Gln Val Tyr Val 1090 1095 1100 Arg Arg Ala Tyr Arg Ala Tyr Thr Val Gly Glu Val Lys Thr Pro Asn 1105 1110 1115 1120 Ile Lys Asp Ser Ser Ile Ser Pro Leu Ile Glu Trp Lys Phe Gln Leu 1125 1130 1135 Pro Ser Ala Ala Phe Ser Ser Ile Pro Gln Val Lys Ser Lys Ile Gly 1140 1145 1150 Met Asn Arg Ala Met Ser Val Ser Asp Leu Ser Tyr Val Val Asp Asn 1155 1160 1165 Glu His Ala Pro Leu Arg Thr Gly Ile Leu Val Ala Ser Glu His Leu 1170 1175 1180 Asp Asp Val Asp Ala Cys Leu Ala Gln Gly Leu Glu Ala Ile Pro Glu 1185 1190 1195 1200 His Ala Asn Thr Ala Asn Gly Pro Ala Pro Asp Arg Ser Gly Asn Ser 1205 1210 1215 Ser Ser Leu Ser Asn Val Ala Asn Val Cys Val Ser Ser Thr Glu Gly 1220 1225 1230 Phe Glu Ser Glu Lys Asp Ile Leu Ala Arg Val His Glu Ile Leu Asp 1235 1240 1245 Leu Asn Lys Ser Asp Leu Ile Lys Ser Ser Ile Arg Arg Ile Thr Phe 1250 1255 1260 Met Phe Gly Phe Ala Asp Gly Thr Tyr Pro Lys Tyr Tyr Thr Phe Asn 1265 1270 1275 1280 Gly Pro Asn Tyr Gln Glu Asn Gln Thr Ile Arg His Ile Glu Pro Ala 1285 1290 1295 Cys Ala Phe Gln Leu Glu Leu Gly Arg Met Ser Asn Phe Asn Ile Lys 1300 1305 1310 Pro Ile Phe Thr Glu Asn Arg Asn Ile His Val Tyr Glu Ala Ile Ser 1315 1320 1325 Lys Thr Ser Ala Leu Asp Lys Arg Phe Phe Thr Arg Gly Ile Ile Arg 1330 1335 1340 Thr Gly Arg Ile Arg Asp Glu Ile Ser Ile Gln Glu Tyr Leu Thr Ser 1345 1350 1355 1360 Glu Ala Asn Lys Leu Met Ser Asp Ile Leu Asp Asn Leu Glu Ile Val 1365 1370 1375 Asp Thr Ser Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ser Ala 1380 1385 1390 Val Phe Asp Val Ser Pro Glu Asp Val Glu Ala Ala Phe Gly Gly Phe 1395 1400 1405 Leu Glu Arg Phe Gly Lys Arg Phe Leu Arg Leu Arg Val Ser Ala Ala 1410 1415 1420 Glu Ile Arg Ile Ile Ile Gln Asp Pro Lys Thr Gly Ala Ala Val Pro 1425 1430 1435 1440 Leu Arg Ala Leu Ile Asn Asn Val Ser Gly Tyr Val Val Lys Ser Glu 1445 1450 1455 Leu Tyr Thr Glu Ile Lys Asn Asp Lys Gly Glu Trp Val Phe Lys Ser 1460 1465 1470 Leu Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr 1475 1480 1485 Pro Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met 1490 1495 1500 Gly Thr Thr Tyr Leu Tyr Asp Phe Pro Glu Leu Phe His Gln Ala Ala 1505 1510 1515 1520 Ile Thr Thr Trp Glu Lys Tyr Leu Ser Asn Ser Ser Ala Lys Glu Leu 1525 1530 1535 Ser Ser Ile Lys Asp Arg Asp Asp Leu Phe Ile Ser His Glu Leu Ile 1540 1545 1550 Glu Asp Glu Asn Gly Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala 1555 1560 1565 Asn Thr Ile Gly Met Val Ala Phe Lys Val Thr Met Lys Thr Pro Glu 1570 1575 1580 Tyr Pro Arg Gly Arg Gln Phe Val Ile Val Ala Asn Asp Ile Thr Phe 1585 1590 1595 1600 Lys Ile Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val 1605 1610 1615 Thr Glu Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala 1620 1625 1630 Asn Ser Gly Ala Arg Ile Gly Ile Ala Glu Glu Leu Val Pro Leu Phe 1635 1640 1645 Gln Val Ala Trp Lys Asp Ser Lys Val Pro Ala Lys Gly Phe Glu Tyr 1650 1655 1660 Leu Tyr Leu Ser Lys Glu Gly Met Glu Thr Leu Lys Ser Tyr Ser Lys 1665 1670 1675 1680 Gln Asn Ser Val Ile Thr Glu Arg Val Val Glu Glu Gly Glu Glu Arg 1685 1690 1695 Tyr Val Ile Lys Ser Ile Ile Gly Ala Asp Asp Gly Leu Gly Val Glu 1700 1705 1710 Cys Leu Arg Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr 1715 1720 1725 Gln Asp Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly Ile 1730 1735 1740 Gly Ala Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Ile Glu Gly 1745 1750 1755 1760 Gln Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys Val Leu Gly 1765 1770 1775 Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile Met 1780 1785 1790 Tyr Asn Asn Gly Val Ser His Leu Thr Ala Ser Asp Asp Leu Gln Ala 1795 1800 1805 Val Glu Gln Ile Ile Asn Trp Leu Ser Tyr Val Pro Ala Lys Arg Asn 1810 1815 1820 Met Pro Val Pro Ile Leu Glu Thr Glu Asp Lys Trp Asp Arg Ala Ile 1825 1830 1835 1840 Asp Phe Arg Pro Thr Thr Asn Glu Gln Tyr Asp Val Arg Trp Leu Ile 1845 1850 1855 Gln Gly Arg Glu Leu Asp Asn Gly Asp Phe Glu Tyr Gly Met Phe Asp 1860 1865 1870 Lys Gly Ser Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val 1875 1880 1885 Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Ile Gly Val Ile Gly Val 1890 1895 1900 Glu Thr Arg Thr Ile Glu Asn Val Ile Pro Ala Asp Pro Ala Asn Pro 1905 1910 1915 1920 Asp Ser Thr Glu Thr Val Val Gln Glu Ala Gly Gln Val Trp Tyr Pro 1925 1930 1935 Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile Gln Asp Phe Asn His Gly 1940 1945 1950 Glu Gln Leu Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly 1955 1960 1965 Gly Gln Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe Ile 1970 1975 1980 Val Asp Ala Leu Val Asp Tyr Lys Gln Pro Ile Phe Ile Tyr Ile Pro 1985 1990 1995 2000 Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp Val Val Val Asp Pro Thr 2005 2010 2015 Ile Asn Asn Asp His Met Glu Met Tyr Ala Asp Lys Asp Ser Arg Ala 2020 2025 2030 Gly Val Leu Glu Pro Ser Gly Met Val Gly Ile Lys Tyr Arg Arg Glu 2035 2040 2045 Lys Leu Leu Ser Thr Ile Thr Arg Leu Asp Ser Thr Tyr Arg Glu Leu 2050 2055 2060 Lys Glu Lys Leu Ser Asp Lys Leu Leu Ser Thr Glu Glu His Gln Lys 2065 2070 2075 2080 Ile Ser Ile Gln Leu Ala Glu Arg Glu Lys Met Leu Ile Pro Ile Tyr 2085 2090 2095 Asn Gln Ile Ser Val Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg 2100 2105 2110 Met Leu Lys Lys Gly Val Ile Lys Ala Glu Leu Thr Trp Glu Glu Ser 2115 2120 2125 Arg Arg Tyr Phe Phe Trp Arg Leu Arg Arg Arg Leu Asp Glu Glu Tyr 2130 2135 2140 Leu Leu Arg Arg Leu Asp Ser Gln Leu Thr Gln Ala Ser Arg Leu Glu 2145 2150 2155 2160 Lys Ile Ala Arg Val Arg Ser Trp Tyr Pro Thr Ser Val Asp Val Glu 2165 2170 2175 Asn Asp Lys Glu Val Val Thr Trp Ile Glu Glu Asn Tyr Glu Thr Leu 2180 2185 2190 Glu Glu Lys Phe Lys Ala Leu Lys Leu Glu Ser Phe Ala Leu Asp Leu 2195 2200 2205 Ala Lys Asn Ile Arg Asn Asp His Glu Asn Ser Met Lys Gly Leu Glu 2210 2215 2220 Glu Val Val Arg Met Leu Ser Ala Thr Asp Lys Glu Arg Leu Asn Lys 2225 2230 2235 2240 Ile Ile Asn Pro Asp Asp Lys Lys 2245 <210> 2 <211> 2243 <212> PRT <213> Candida humilis <400> 2 Met Ser Glu Glu Asn Leu Phe Glu Asp Ala Ala Ala Lys Arg Gln Tyr 1 5 10 15 Glu Ile Thr Asp Tyr Ser Thr Lys His Lys Thr Leu Ala Ser His Phe 20 25 30 Val Gly Leu Asn Thr Leu Glu Arg Val Glu Glu Ser Pro Leu Lys Asp 35 40 45 Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile 50 55 60 Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys 65 70 75 80 Trp Ala Tyr Glu Thr Phe Gly Asp Glu Arg Thr Val Gln Phe Val Ala 85 90 95 Met Ala Thr Pro Glu Asp Leu Gln Ala Asn Ser Glu Tyr Ile Arg Met 100 105 110 Ala Asp Gln Tyr Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr 115 120 125 Ala Asn Val Asp Leu Ile Val Asp Val Ala Glu Arg Thr Asp Val Asp 130 135 140 Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro His Leu Pro 145 150 155 160 Glu Lys Leu Ala Ala Ser Lys Arg Lys Ile Ile Phe Ile Gly Pro Pro 165 170 175 Gly Ser Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val 180 185 190 Ala Gln His Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val 195 200 205 Asp Thr Val His Leu Asp Ser Glu Thr Gly Leu Val Ser Val Asp Asp 210 215 220 Asp Ile Tyr Gln Lys Gly Cys Cys Ser Ser Pro Glu Asp Gly Leu Ala 225 230 235 240 Lys Ala Lys Lys Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly 245 250 255 Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile 260 265 270 Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe 275 280 285 Ile Met Lys Leu Ala Gly Lys Ala Arg His Leu Glu Val Gln Leu Leu 290 295 300 Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser 305 310 315 320 Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile 325 330 335 Ala Ser Asn Glu Thr Phe Glu Lys Met Ala Ser Ala Ala Val Arg Leu 340 345 350 Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr 355 360 365 Ser Ser Glu Glu Lys Lys Tyr Tyr Phe Leu Glu Leu Asn Pro Arg Leu 370 375 380 Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro 385 390 395 400 Ser Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser 405 410 415 Asp Ile Arg Val Leu Tyr Gly Met Asn Pro His Gly Ala Thr Glu Ile 420 425 430 Asp Phe Glu Phe Lys Thr Glu Glu Ala Lys Lys Thr Gln Arg Lys Pro 435 440 445 Val Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro 450 455 460 Asn Glu Gly Phe Lys Pro Ser Gly Gly Ser Leu His Glu Leu Asn Phe 465 470 475 480 Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly 485 490 495 Gly Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe 500 505 510 Gly Glu Asn Arg Gln Met Ser Arg Lys His Met Val Val Ala Leu Lys 515 520 525 Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile 530 535 540 Lys Leu Leu Glu Thr Glu Asp Phe Glu Glu Asn Thr Ile Thr Thr Gly 545 550 555 560 Trp Leu Asp Asp Leu Ile Ser His Lys Met Thr Ala Gln Lys Pro Asp 565 570 575 Ala Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Ile Ala 580 585 590 Ser Glu Ser Ala Arg Lys Glu Tyr Ile Asn Gln Leu Lys Arg Gly Gln 595 600 605 Val Pro Pro Lys Gly Leu Leu Glu Thr Met Phe Pro Ile Glu Phe Ile 610 615 620 His Glu Asn Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Ala Asp Asp 625 630 635 640 Ser Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Glu Val Arg Ala Val 645 650 655 Lys Leu Ser Asp Gly Gly Leu Leu Ile Ser Val Gly Gly Gln Ser His 660 665 670 Thr Ile Tyr Trp Lys Glu Glu Ala Gln Ala Thr Arg Leu Ser Ile Asp 675 680 685 Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg 690 695 700 Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Asp 705 710 715 720 Gln Ile Lys Val Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met 725 730 735 Gln Met Pro Leu Val Ser Gln Glu Asn Gly Val Ile Gln Leu Leu Lys 740 745 750 Gln Pro Gly Ser Thr Ile Asp Ala Gly Asp Ile Ile Ala Met Leu Ala 755 760 765 Leu Asp Asp Pro Ser Lys Val Lys His Ala Met Pro Phe Glu Gly Met 770 775 780 Leu Pro Glu Leu Gly Ser Pro Met Val Glu Gly Thr Lys Pro Ala Tyr 785 790 795 800 Lys Phe His Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr 805 810 815 Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val 820 825 830 Leu Arg Asp Pro Arg Leu Pro Tyr Ser Glu Trp Lys Leu Asn Val Ser 835 840 845 Ala Leu His Thr Arg Leu Pro Leu Ala Leu Asp Glu Lys Leu Asp Lys 850 855 860 Leu Val Glu Arg Ser Ala Asn Arg Ser Ala Ile Phe Pro Ala Lys Gln 865 870 875 880 Leu Gln Lys Met Ile Asp Asp Ala Val Arg Asp Pro Gln Ala Asp Pro 885 890 895 Leu Leu Ser Ser Ile Val Glu Pro Leu Leu Asp Ile Thr Thr Arg Tyr 900 905 910 Ala Asn Gly Leu Glu Ala His Glu His Ser Val Phe Val Lys Phe Leu 915 920 925 Glu Glu Tyr Tyr Asn Val Glu Lys Leu Phe Ser Glu His Gly Glu Arg 930 935 940 Ala Glu Asn Val Ile Leu Lys Leu Arg Asp Glu Asn Ile Asp Asp Leu 945 950 955 960 Asp Lys Val Val Leu Ile Val Leu Ser His Ala Lys Val Ser Ala Lys 965 970 975 Asn Thr Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Ile Cys Lys 980 985 990 Met Ser Ser Ser Val Ala His Ile Phe Thr Lys Pro Leu Gln His Ile 995 1000 1005 Val Glu Leu Glu Ser Lys Pro Ala Ala Lys Val Ala Leu Gln Ala Arg 1010 1015 1020 Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg Thr Glu 1025 1030 1035 1040 Gln Ile Glu His Ile Leu Arg Ser Ser Val Thr Asn Thr Ser Tyr Gly 1045 1050 1055 Asp Ser Ser Pro Lys Val Ser Glu Pro Asn Met Glu Ile Leu Lys Asp 1060 1065 1070 Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val Leu Leu Pro Phe Leu 1075 1080 1085 Cys His Glu Asp Pro Val Ile Ala Thr Ala Ala Gly Gln Val Tyr Val 1090 1095 1100 Arg Arg Ala Tyr Arg Ala Tyr Thr Val Gly Glu Val Lys Ser His Ser 1105 1110 1115 1120 Ile Thr Asp Ser Ser Met Asn Pro Leu Ile Glu Trp Lys Phe Gln Leu 1125 1130 1135 Pro Ser Ala Ala Phe Ser Ser Ile Pro Gln Leu Lys Ser Lys Ile Gly 1140 1145 1150 Met Asn Arg Ala Met Ser Val Ser Asp Leu Ser Tyr Val Val Asp Ser 1155 1160 1165 Glu His Ala Pro Leu Arg Thr Gly Ile Leu Val Ala Ala Glu His Leu 1170 1175 1180 Asp Glu Val Asp Ala Ser Leu Ala Gln Gly Leu Glu Val Ile Pro Glu 1185 1190 1195 1200 His Ser Ala Pro Gly Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser 1205 1210 1215 Ser Ser Leu Ser Asn Ile Val Asn Val Cys Val Ala Ser Thr Glu Gly 1220 1225 1230 Phe Asp Ser Glu Lys Glu Ile Leu Ala Arg Val Asn Glu Ile Leu Glu 1235 1240 1245 Leu Asn Lys Ala Asp Leu Ile Lys Ser Ser Ile Arg Arg Ile Thr Phe 1250 1255 1260 Met Phe Gly Phe Pro Asp Gly Thr Tyr Pro Lys Tyr Tyr Thr Phe Asn 1265 1270 1275 1280 Gly Pro Asn Tyr Gln Glu Asn Gln Thr Ile Arg His Ile Glu Pro Ala 1285 1290 1295 Cys Ala Phe Gln Leu Glu Leu Gly Arg Met Ser Asn Phe Asn Ile Thr 1300 1305 1310 Pro Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr Glu Ala Val Ser 1315 1320 1325 Lys Thr Ser Ala Leu Asp Lys Arg Phe Phe Thr Arg Gly Ile Ile Arg 1330 1335 1340 Thr Gly Arg Ile Arg Asp Glu Ile Ser Ile Gln Glu Tyr Leu Thr Ser 1345 1350 1355 1360 Glu Ala Asn Lys Leu Met Ser Asp Ile Leu Asp Asn Leu Glu Ile Ile 1365 1370 1375 Asp Thr Ser Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ser Ala 1380 1385 1390 Val Phe Asp Val Ser Pro Glu Asp Val Glu Ala Ala Phe Gly Gly Phe 1395 1400 1405 Leu Glu Arg Phe Gly Lys Arg Phe Leu Arg Leu Arg Val Ser Ala Ala 1410 1415 1420 Glu Ile Arg Ile Ile Ile Gln Asp Pro Lys Thr Gly Ala Pro Val Pro 1425 1430 1435 1440 Leu Arg Ala Leu Ile Asn Asn Val Ser Gly Tyr Val Val Lys Ser Glu 1445 1450 1455 Leu Tyr Thr Glu Ile Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser 1460 1465 1470 Leu Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr 1475 1480 1485 Pro Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met 1490 1495 1500 Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe His Gln Ala Ala 1505 1510 1515 1520 Ile Thr Thr Trp Gln Lys Tyr Lys Thr Asn Ser Ser Gln Lys Asp Leu 1525 1530 1535 Thr Thr Met Lys Glu Arg Asp Asp Leu Phe Ile Ser His Glu Leu Ile 1540 1545 1550 Glu Asp Glu Ser Gly Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala 1555 1560 1565 Asn Ser Ile Gly Met Val Ala Phe Lys Val Thr Met Lys Thr Pro Glu 1570 1575 1580 Tyr Pro Arg Gly Arg Gln Phe Val Ile Val Ala Asn Asp Ile Thr Tyr 1585 1590 1595 1600 Lys Ile Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val 1605 1610 1615 Thr Glu Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala 1620 1625 1630 Asn Ser Gly Ala Arg Ile Gly Ile Ala Glu Glu Leu Val Pro Leu Tyr 1635 1640 1645 Gln Val Ala Trp Lys Asp Ala Asp Val Pro Ala Lys Gly Phe Glu Tyr 1650 1655 1660 Ile Tyr Leu Asn Lys Asp Glu Met Glu Thr Leu Lys Gly His Ser Lys 1665 1670 1675 1680 Glu Asn Ser Val Ile Thr Glu Arg Ile Val Glu Asp Gly Glu Glu Arg 1685 1690 1695 Phe Val Ile Lys Ala Ile Ile Gly Ala Asp Asp Gly Leu Gly Val Glu 1700 1705 1710 Cys Leu Arg Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr 1715 1720 1725 Gln Asp Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly Ile 1730 1735 1740 Gly Ala Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Ile Glu Gly 1745 1750 1755 1760 Gln Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys Val Leu Gly 1765 1770 1775 Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile Met 1780 1785 1790 Tyr Asn Asn Gly Val Ser His Leu Thr Ala Ser Asp Asp Leu Gln Ala 1795 1800 1805 Val Glu Gln Ile Ile Lys Trp Leu Ser Tyr Val Pro Ala Lys Arg Asn 1810 1815 1820 Met Pro Val Pro Ile Leu Glu Asn Gly Asp Lys Trp Asp Arg Gln Ile 1825 1830 1835 1840 Asp Tyr Arg Pro Thr Val Asn Glu Gln Tyr Asp Val Arg Trp Met Ile 1845 1850 1855 Glu Gly Arg Glu Leu Glu Asp Gly Arg Phe Glu Tyr Gly Met Phe Asp 1860 1865 1870 Lys Gly Ser Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val 1875 1880 1885 Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Ile Gly Val Ile Gly Val 1890 1895 1900 Glu Thr Arg Thr Ile Glu Asn Val Ile Pro Ala Asp Pro Ala Asn Pro 1905 1910 1915 1920 Asp Ser Thr Glu Ala Val Val Gln Glu Ala Gly Gln Val Trp Tyr Pro 1925 1930 1935 Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile Lys Asp Phe Asn His Gly 1940 1945 1950 Glu Gln Leu Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly 1955 1960 1965 Gly Gln Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe Ile 1970 1975 1980 Val Asp Ser Leu Val Asp Tyr Lys Gln Pro Ile Phe Ile Tyr Ile Pro 1985 1990 1995 2000 Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp Val Val Val Asp Pro Thr 2005 2010 2015 Ile Asn Ala Asp Gln Met Glu Met Tyr Ala Asp Lys Asp Ser Arg Ala 2020 2025 2030 Gly Val Leu Glu Pro Ser Gly Met Val Gly Ile Lys Tyr Arg Arg Glu 2035 2040 2045 Lys Leu Leu Ala Thr Met Thr Arg Leu Asp Thr Thr Tyr Arg Glu Leu 2050 2055 2060 Lys Glu Gln Leu Ser Asp Lys Thr Leu Thr Pro Ala Gln His Gln Glu 2065 2070 2075 2080 Val Ser Val Lys Leu Ala Glu Arg Glu Lys Phe Leu Leu Pro Ile Tyr 2085 2090 2095 Thr Gln Val Ser Val Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg 2100 2105 2110 Met Val Lys Lys Gly Val Ile Arg Ala Glu Leu Thr Trp Glu Glu Ser 2115 2120 2125 Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg Lys Leu Asp Glu Glu Tyr 2130 2135 2140 Leu Val Lys Arg Leu Asp Ile Glu Leu Pro His Ala Ser Arg Leu Glu 2145 2150 2155 2160 Lys Leu Ala Arg Leu Arg Ser Trp Tyr Pro Ala Ser Ile Asp Glu Thr 2165 2170 2175 Lys Asp Arg Asp Val Val Thr Trp Ile Glu Glu Asn Tyr Asn Val Phe 2180 2185 2190 Glu Glu Glu Phe Lys Ala Leu Lys Leu Glu Ser Phe Ala Gln Glu Leu 2195 2200 2205 Ala Lys Ser Met Arg Thr Asp His Asp Val Thr Met Arg Gly Leu Glu 2210 2215 2220 Glu Ile Ala Arg Met Leu Ser Lys Glu Asp Lys Glu Arg Leu Leu Ser 2225 2230 2235 2240 Ala Leu Lys <210> 3 <211> 1443 <212> DNA <213> AADHab <400> 3 atggaaatta acgaaaaaga tttgaagttg attattgaaa acgtattgga caagttagaa 60 agtgacaaga gtgataaaaa gatattcgat tcttcattga atattccatc cagtggtcaa 120 gacggtatat ttgaagaagt caacgctgca gtagatgccg ctgaagcagc ccatttggaa 180 ttaatgaaat tgacattaga aaagagaaga gaaataatca gatctataag aaagatcata 240 atgtctaatt tggaagaaat ctcaaagtta gctgtcgaag aaactacatt cggtagagta 300 gaagataaga tcgaaaagaa cagattggct gcattaaaaa caccaggtat tgaagatttg 360 gaacctaccg catactcaga tgacaacggt atgactttga tggaaagagc cgcttacggt 420 gttataggtt ccataatccc aagtactaac cctacatctt cagttatcaa taacggtatt 480 tccatgatag ctggtggtaa tagtgttgtc tttaacccac atcctgcagc caaaagatcc 540 agttgtttca caatctcctt gattaatcaa gcaatagtta aggccggtgg tccacctaat 600 gtcttgtgcg ccattgctaa cccaactata gattctgcac aaacattgat gaagcaccca 660 aaaattaagt tgttagtagt taccggtggt cctgcagtcg taaagactgc tatgaactct 720 gataaaaagg ttattgctgc aggtccaggt aacccacctt gtgttgtcga tgaaactgct 780 gacttagtta aagcaggtag agatattgtc aatggtgctg gttttgacaa taacatagtt 840 tgtatctgcg aaaaggaaat attggccgtt tctcaaatcg ctgatcaatt gaaggaagaa 900 atgattaaga atggtgccca tgaattgaag ggtgaacaaa tcgaaaaggt tacaaagtta 960 gtcattaccg atccaggtaa acctggtcac gaaggtgcac caaataagaa atacgtcggt 1020 aaaaatgctt ctgtaattgc aagagatata ggtttagaca tctcagaaca aacaaagata 1080 ttgttgtgtg aagtagatag ataccatcct ttggtttgga ccgaacaatt gttaccagta 1140 attccattgg ttagattcaa taacgttgat gaagccatag actttgctgt tcaatgtgaa 1200 cataatttca gacactctgc ttcaatccac tctaaaaaca ttgcaacatt gtcaagaatg 1260 gccaagttga tgaactgctc cttattcatt aagaatggtc caaactacag tggtttaggt 1320 tttggtggtg ctggttacac ctcattcact atagcaaccc ctactggtga aggtttgact 1380 agagctagaa cttttactag agaaagaaga tgtgtattag ttggttactt cagaattgtt 1440 tga 1443 <210> 4 <211> 1434 <212> DNA <213> AADHal <400> 4 atggtagatc aaaccttaat agaacaaata acaagagccg tattgaccca aatgaaagct 60 ggtaaagacg ccgccgtatc cggtgacggt atttttgcca cagttgatca agctgtcgct 120 gcagccagac aagcttatca agaattgaga ttgttaacct tggaaaagag agaaactttg 180 ataagagcta tcagagatgc tgcattcgca aatgccgctg taattgccca aatggctgtt 240 caagaatccg gtatgggtag agttgaagat aagatcttga agaaccaatt agcagccaga 300 aaaaccccag gtactgaaga tttgacttct agagcttggt caggagatca tggtttgaca 360 ttagtagaaa tggcccctta cggtgttatt ggtgctataa caccaactac aaatccttct 420 gaaaccgtta tttgtaactc aatcggtatg attgctgcag gtaattccat agtttttagt 480 ccacacccta ctgctcaaaa tacatccttg accactatca gattgttaaa cgaagctatt 540 gtcaaagcag gtggtccaga taatttgtta acagccgttg ctgaacctag tatcgaagcc 600 gctaacgcaa tgatgagaca tccaggtatt caaatgttgg ttgctactgg tggtcctgcc 660 gttgtcaagg ctgtcttatc ttcaggtaaa aaggcaattg gtgcaggtgc cggtaatcca 720 cctgccgtag ttgatgaaac tgcagacata gccaaagcag ccaaggatat cgttgcaggt 780 tgttcattcg acaataactt gccatgcatt gctgaaaagg aaattatagc agttggtaga 840 atagccgatg aattgatctc ctatttgcaa aagtacggtg catacttaat aagtggtaga 900 gacatagaaa gattgatgga agtcgtatta acagaaagaa ccgaagaaat ggctccaggt 960 tgtgttggta aacctagaag agtctacggt gtaaataagg attacattgg taaagacgcc 1020 aagtttatat tgtctaaaat caacatacaa gctccagatc atataagagt tatcttatgc 1080 gaaacaccag ctgaccaccc tttcgtcttg gaagaattaa tgatgccagt attgccttta 1140 gtttcagtca gagatattga cgctgcaata gatttggcag taaaagttga acatggtaat 1200 agacacaccg ctgtcatgca ttctaagaac gtagactata tgactagatt agctaaggca 1260 atcgaaacaa ccatattcgt aaagaatgca ccatcatacg ccggtatagg tgttggtggt 1320 gaaggtttta ctacttttac tattgctggt cctactggtg aaggtttgac atccgcaaga 1380 agttttacta gacaaagaag atgcgcttta gttgatgcat tctctattgt ctga 1434 <210> 5 <211> 1395 <212> DNA <213> AADHbs <400> 5 atggatgaaa taatgataaa gaacatagta agagaagtat tgatgaaaac taatttggat 60 atctcctgta agagtgacca aggtgtattt gaagatgtta atgacgcaat tgatgctgca 120 tatattgccc aaagagactg gtatatgaac tacaaggtcg aagatagaag aagaatcatc 180 gaagctatca gagttgtcgc tagagaaaat gcaccaatgt tcgccaagat gattcatgaa 240 gaaaccggta tgggtagata cgaagataag atcactaaga acttggcagt tatagataaa 300 actccaggtg ttgaatgttt ggtcacagac gccatctctg gtgactcagg tttaatgatt 360 gaagaacaag ctccttttgg tgttattggt gcaataactc catctacaaa ccctacagaa 420 accatcatta ataacacaat ctcaatgata ggtggtggta atgcagtagt tttcaacgtt 480 catccaggtg ctaaaaaggt ctgtgcagta tgcttgcaaa tcttgcacaa gactatcgtt 540 gaaaacggtg gtccagctaa cttaataaca atgcaaagaa agcctgatat ggaagccgtt 600 aataagttga cagcttcccc taagattaga ttaatggttg gtaccggtgg tatgggtatg 660 gttaacgcat tgttgaagtc tggtaaaaag acaattggtg ctggtgcagg taacccacct 720 gtcgtagttg atgacaccgc tgatttggac aaagccgctt ccgaaatata tagaggtgcc 780 agtttcgata acaacttgtt gtgtttggct gaaaaggaaa ctttcgtaat ggacaatgtt 840 gctgatgaat tgattagaaa gatgtgtgcc tgcggtgctc atttgataac cccacaagaa 900 actgaacaat tgttgaaagt tattttcttg gaaaaggacg gtaaatactc tgttaacaaa 960 aagtgggtcg gtaaagatgc ttctttgatc ttagaatcaa taggtatcaa ggatgcagac 1020 acaagattgg tcttatgcga agtaccacat gatcaccctt ttgttttggt cgaacaatta 1080 atgccaataa tgcctatcgt cagatgtaaa actttcgaag attgcgtaaa gtatgcagtc 1140 gtagccgaaa atggtaacag acataccgcc tccatgttca gtaaaaatgt tgatcacatg 1200 actagattcg ctaaggaaat cgaaactaca atattcacaa agaacgcagc caccttggta 1260 ggcgttggta taggtggtga aggtcacacc actatgacta tcgcaggtcc tacaggtgaa 1320 ggtttaacct gtgccagatc ttttactaga agaagaagat gtatgttggc tgatggtggt 1380 ttaagagtta tttga 1395 <210> 6 <211> 1455 <212> DNA <213> AADHbw <400> 6 atggacgtca gacaacaaga tgtagaaaga atcgtagtag aagtattgaa aaagatgatg 60 agtgatcaac caaccgctgc tgccacaaca gttgtcgctg catccggttg tgattgcggt 120 gactttggtt tgttcgatag attggaagat gctgtccaag ccgctgaagc agcccaaaag 180 aaaattagta cagtagcaat gagagataag attatagctg caataagaaa ggctggtttg 240 gaaaatgcca aagcttttgc agaaattgca cataacgaaa ccggtatggg tagagtctct 300 gataagatcg ctaagaatat cttggtatgc gaaagaactc ctggtacaga atgcttatcc 360 ccaatggcaa ttagtggtga catgggtttg actttaatag aaaatgcacc ttggggtgta 420 atcgcctctg ttaccccttc aactaaccca accgctactg ttataaataa cgccatctcc 480 atgattgctg gtggtaatag tgttatcttt gctccacatc ctaacgctaa gagagcatct 540 caaactgcaa tccaagtatt gaataaggct atcatagaag caacaggtgt tgccaacttg 600 ttagtcgctg taaaagaacc taccattgaa gttgcacaag aattattctc acacccaaga 660 attaaattgt tagtagttac tggtggtgaa gccgtcgtag cccaagctag aaaagttgct 720 acaatgagat tgattgccgc tggtgcaggt aatccacctg ttgtcgtaga tgaaacagcc 780 aacattgcta gagcagccag atctatatat gatggtgcct cattcgacaa taacatcatc 840 tgtgctgacg aaaaggaaat tatagcagtt gattctattg ccgaccaatt aaaagctgaa 900 atgaaggcaa ttggtgccgt tgaaatatca ttggaacaag cagatgccgt cgctagagtt 960 gtcttaagaa attaccctca agttgaaggt ggtaaagctc caaatcctaa cccaaaatgg 1020 gtcggtagag atgctgcatt gatagcaaag gccgctggta ttgatgttcc agactcctgc 1080 agattgttaa tcgttgatgt caagagagac ataaaccatg tctttgctag agtagaacaa 1140 ttgatgcctg taattccatt gttgagagca gccaacgttg atgaagctat cgaatgggca 1200 ttgattttag aaagaggttt gtctcatacc gctggtatgc actcaagaaa tattgataac 1260 atggacaaga tggcaagagc catgaacact tcattattcg ttaaaaacgg tcctcacttg 1320 gctgcattag gtgctggtgg tgaaggttgg actacaatga caatttccac accaaccggt 1380 gaaggtgtta cctgtgctag aagttttgtc agattaagaa gatgttgcgt agttgataac 1440 ttcagaatag tttga 1455 <210> 7 <211> 1314 <212> DNA <213> AADHcs <400> 7 atgaagtcct ccatatactc cagtgtttcc gaagctgtaa tggctgctag aggtgctttt 60 aatagatacg ccgatttgac cttgaatgaa agaaaagaaa ttatagaagc tgtcaaggct 120 gcagtaagac caagaattac acaattggca gaaatgaccg ccgctgaaac tggtatgggt 180 aacgttgcag ataagatcga aaagatcaga ttggccgtcg aaaagactcc aggtgtagaa 240 gatttgatta cagaagtaaa caccggagat catggtatga ctttgtatga attatcagca 300 tttggtgttg tctgtgccgt tcacccatgt actaatcctt gcgctacatt gatttccaac 360 accatcggta ttttagcagc cggtaatgct gtagttcatt gtccacaccc tagagcagtt 420 agagtctctc aatacttaac tggtatgata tctgatgctg ttagagatgt ttgcggtatt 480 gataacttgg tcgtaactgt tggtgaatcc ttaatgggta gtacaagaga aatcatgtca 540 catccagatg ttgacttgat tgtctgcact ggtggttccg gtcctttaag acaagccatg 600 agtgctggta aaaaggtcat tggtgcaggt ccagccaatc ctgtagctat agttgatgaa 660 acagcagact tggaaagagc tgcaagagat attgttaaag gtgcttcttt cgacaataac 720 ttaatgtgta cctccgaaaa atctattgta gccgttgaaa agatagctga tgaattgatc 780 tctcatttgg aaagaaacgg tgtttactac actagatcag cagaagaaat agatagattg 840 tattgcaccg ccgtaactga agatttcgtt atcaacaaaa agatggaagg tcaatctgct 900 gatgttattt tggacgccgc taagatccca catagaaagg gtattaagtt gatcgtcgcc 960 gaaacagata gaacccaccc tttcgctaca ttggaattgt tgatgccatt gttgccttta 1020 gttagagcca gagattttga cgaagctttg gaagcagcct tagatatcga acaaggtttc 1080 agacataccg ctactattca ctctgaatca gttgaaagaa tgaatagagc tgctaagaaa 1140 ttgcaaactg cagtctttgt taaaaatggt ccatccttgt taggtattgg ttttgataag 1200 gaaggtcata cttcttttac tatcggtaca attaccggtg aaggtactac atctgctaga 1260 cactttgcta gaagaagaag atgtacttta acatctggtt tctcaattag atga 1314 <210> 8 <211> 1458 <212> DNA <213> AADHho <400> 8 atggcattga gagaagatag aatagcagaa atagtagaaa gagttttagc aagattggat 60 ggtaacagtg gttcctccgc cgcacctcat tctggttcag gtgctccagc tgcaaccgca 120 ggtggtgcct ctttggatat tcctagaggt actttaggtg tttatgctga tgcagacgcc 180 gctgtcaatg cagccagaag aggttttgct gcaaacgaag ctttgccatt aagaactaga 240 caagcaatga tagatgccat gagaaaagtt gctagagcac atatccctga attggctaga 300 tatgcagttg ccgaaacagg tttaggtaga tacgaagata aattggcaaa gaatgaatta 360 gttattgcca agacaccagg tcctgaaata ttggctccag ttgcatatac cggtgacgat 420 ggtttgacct taactgaaag agcaccatac ggtgttatag gtgcaatcac tccatgtaca 480 aatcctacag aaaccgttat ttgcaacgca ataggcatgt tgtccggtgg taatgccgtt 540 gtttttaatg ttcatccaag tgccgctaga gtctgtaatt ggttggtaca cttgttaaac 600 gaagcaatta tgtccgtcgg tggtccaaga gatgctatca cctccgtaga aagtcctact 660 attgacagtg cccaaacctt gatgactcat gctggtgtta gattagtagt tgtcactggt 720 ggtccaggtg tagttagagc agccatgaaa tctggtaaaa aggttatcgc tgcaggtcct 780 ggtaatccac ctgctgtcgt agacgaaact gccaacttgg ctaaagccgc tgcagccatt 840 ataaagggtg catcaataga taacaacatc atttgcacag ctgaaaaaga aatagtagct 900 gttgcttcta ttgctgacga attaagtaga ttgttaggtc aaagaggtgc tttggtatta 960 ggtgacgctc aagttagagc attggaaaga gttgtcttag acggtgaaca tgttaataag 1020 gaatgggtcg gtaaagatgc ctctagaatc gctgaacaaa ttggtttgag aggtcacggt 1080 tcagatttga gattgttagt atgtccagtt gacgaaggtc atccttttgt acaacacgaa 1140 ttgttaatgc cagttattgg tttagtcaga gtatccgatg ctactgaagc tatggctaca 1200 gcagttagag tcgaacatgg tttttgccac acagctgtta tgcattctac ccacattgac 1260 agattgtcag ctatggctag agtttgtaac gcatcaatat tcgttaaaaa tgattgcaac 1320 ttagctggtt tgggtttagg tggtgaaggt tttacatctt tcaccattgc atcaccaaca 1380 ggtgaaggtt tgactacagc tagagatttc actagagtta gaagatgtac attgaaggaa 1440 tcttttagat tcgtctga 1458 <210> 9 <211> 1404 <212> DNA <213> AADHhs <400> 9 atgaaaatca aagaaaacga attggataaa atagtcaacc aagtaatcag ttccttaaac 60 aacaagcaaa acagtaacga cttcaacact aagatcaatt acggtatttt ctctacaatg 120 gatgaagcaa ttgccgaagc tgttaaagca caagcctgtt tgcaattaaa ttactccact 180 gaagctagag aaaagattat taaatctatt agaaagaatg tttctaagca tgttgaaaag 240 atttctgaaa tggcagttga agaaacagat atgggtagaa tcgaagataa gatcattaag 300 aataacttgg ctattaataa gactccaggt acagaagatt tgagaaccga agcctttagt 360 ggtaaaaagg gtttgactat tgtcgaagaa gctcctttcg gtgtaatctg ttctattgca 420 ccagttacta atcctaccga aactataatc tctaacgcca tatcaatgat cgcttcctgc 480 aatggtgttg tctttaacag tcacccaggt gctaaaaagg tttctaagta cataatcgaa 540 gtcttgaata aggttattat ggaagccggt ggtccagaaa atttgttaac cgctgtcaac 600 gaacctacct tgcaaactgt agaatcttgt atgagagatg acagaattgc aatgatagtt 660 gccacaggtg gtccaggtgt agttaatgct gcattatctt caggtaaaaa ggctattggt 720 gctggtgcag gtaacccacc tgtcttggta gatgacacag ttgatttgaa gagagttgcc 780 aaagacatca ttaatggtgc ttcattcgat aacaacttac cttgcacttc cgaaaaggcc 840 attgttgctt tggaaagtat agctgattct ttgttaaacg aaatgacaaa ccaaaacgca 900 caattagttc atgacatcaa agccttggaa agagtcattt tgaacgatga cggtagtata 960 aacaaggcat tggttggtaa agatgccgct ttcattttga ataaggctgg tttaaaggca 1020 aagtctgaag atttgagatt agttattgtc gatgtagact taagacaccc atttgtccaa 1080 aaggaacaat tgatgccagt tattccttta gtcagagcta aaaatttcaa cgaagcaatg 1140 gaaatgggtg ttgatattga agaaggtaat agacatacag ctatcatcca ctcaaagaat 1200 gtagataact tgaccaagtt cgctaagaaa attgaaacta caatctatgt taaaaacgcc 1260 ccatcctacg ctggtattgg tgcaggtggt gaaggttacg caacattcac catagccggt 1320 cctacaggtg aaggtttaac ctccgctaga agttttacta gaaaaagaag atgcgttttg 1380 gtcgatggtt tctcaattat atga 1404 <210> 10 <211> 1398 <212> DNA <213> AADHma1 <400> 10 atgcaaaccg atgctcaaca aatagaaagt atagttagaa gagtcataga acaattacat 60 tccccacaaa gagatggtga aagttacggt gtctttagaa ccttggatga cgcagtagcc 120 ggtgctcaag gtgcttacaa aaagataaga actatggctc aaagagaagc aattatagct 180 gcaatcagaa gaactggtag tgaaaatgtt caagcattgt ctgaattagc cgtccaagaa 240 acaggtttcg gtagagtaga agataagatc agaaagcata gattggtttt agacaaaact 300 cctggtatcg aagctattgt tccaatggca gtcacaggag atcatggttt gtctttaatt 360 gaaaatgctc cttggggtgt aatagcatcc gttaccccta gtactaaccc atctgctact 420 atcttgaaca acgcaatatc aatgatcgcc gctggtaatt cagttgtctt ttccccacat 480 cctgcagcca gagctgtctc ccaaagaaca atccaattga taaatagagc ctctgtttca 540 gctggtggtc ctgcaaactt agtcacctgt gtagaagaac caacaattga agctgcaacc 600 agattgtttt cattccctgg tatacaattg ttaaccatca ctggtggtga aggtgtagtt 660 aatgccgcta gaaaagttac tgataagaga ttaatcgcag ccggtccagg taacccacct 720 gtcgtagttg atgaaacagc tgacattgaa agagctgcaa tttcaatagt tcaaggtgca 780 tccttcgata acaacatcat atgtgttgac gaaaaggaaa taatcgccgt cgaatccatt 840 gctactgaat tgaaaactgc tatgtgcaga catggtgctg ctgaaattaa tgcagatcaa 900 gcagacgccg tcgctagatt ggtattagct ggttacccag gtcctaaccc acaccctaaa 960 ccagaatggg ttggtagaga tgctgaaaag attgcagccg ctgcaggttt tagtgtacct 1020 gcaggtacta gattgttagt tacagaaacc gaaagagatc atgcattcgc cactacagaa 1080 atgatgttgc cagttatctc tttgataaga gctagagatg cagaccaagc cattgattgg 1140 gcagttgaat tggaagccgg taatagacat acagccgcta tgcactcaag aaatatcgac 1200 aacttgtcca gaatgggttt agaaattaat tgttctttgt tcgttaaaaa cggtccttgc 1260 ttggccggtt taggtgctgg tggtgaaggt tggacaagta tgaccatatc tactccaaca 1320 ggtgaaggtg taaccaacgc tagtactttc gttagaaaga gaagatgcac aatggttgat 1380 tctttcagaa ttgtctga 1398 <210> 11 <211> 1422 <212> DNA <213> AADHma2 <400> 11 atggccttta atgaagattt gattgcctcc atagtagaac aagttttgaa ggaaatacaa 60 gatgatgacc acgttatcgc ctccgctaga caatccgttg tcaaagcaaa tgataactgg 120 ggtgtctata ctgatgtaga cgaagctatg gaagtagtta ataaggcaca aaaggaattt 180 gctgcaatgc caatatccaa gagagatgaa attataagtg ctatcagaac agcaggtgtt 240 gaaaacgctg aacatttggc aagaattgcc cacgaagtta ccggttatgg tagaatcgaa 300 gataagactg ttaagaatag aaacgccgct atgttaactc caggtataga agatttgcaa 360 aaggaatttt tgtctggtga cggtggttca gtcattgtag aatacggtcc tatgggtgtt 420 atagcttcct tgatgccaag tacccatcct actgcatttg ttataaatca cgctatcgca 480 atgttagcag ccggtaacgc aatattcatc tgtccacatc ctaaagccca agctgcaaca 540 ttggaagcta tgaaggcatt gaataaggcc attgttgaag ctggtggtcc aaaatacttg 600 atggttgcag tcgataaggc cgacatggaa acctccaaca aagtttatgc acatccttac 660 gttaagatgg tcgccgctgc aggtggtgaa ggtgttatca gagccgcttt aaaaagtggt 720 aaaaaggcta ttaccggtgg tccaggtaat ccacctgtcg tagttgatga aactgctgat 780 attgctaaag cagccaagga tatcattgac ggtgaatctt acgataacaa cgttttgtgc 840 atagcagaaa aggaagtctt tgtcgtagaa tcagttgttg atagattgat tgctgaaatg 900 caaaagaata acgcttactt gatcaagggt caagatgtag ctaatttgac aggtgttacc 960 attaaagacg gtaaaccaaa tggtcaattg gtcggtaaaa acccttctgt aattttgaag 1020 gaaataggta tcgaagttgg tgacgatgtt agaactgtta tatttgaagc tccacctgaa 1080 catccattgg taatgatcga acaattgatg ccagttttgc ctatagtaag agttaaggat 1140 ttcgacgaag ccgttaaatg ggcagtcaag gccgaacatg gtgacggtca cacagctata 1200 ttgcattcta acaacatcaa cagaattaca gaatatgcca gagctatgca agtcaccgta 1260 ttagtagtta acgcaccatc ttattcatgt atggacccac aatcaaaaac tttggtttac 1320 gctcacactt taacaggtcc aacaggtgaa ggtttttgca ctcctagaca tttcacaaga 1380 caagaaagaa ttgtcttagg tggtgctttt cacttcgttt ga 1422 <210> 12 <211> 1437 <212> DNA <213> AADHmm <400> 12 atggaccaaa aggaaattga aaacgtagta aaagccgtct tagcctctat gagtgcaggt 60 acacaaccag cagccgcaag tgccgcacca caacaagctg cagcctccca aaataacggt 120 tttggtgtat tcgaaagttt ggatgacgct gttttagctg caaaagaagc acaaaaatcc 180 ttgaaaactg ttgaaatgag aaatttgtgt atcggtgcta tcagaagagc cgctaccgaa 240 catgcaagag aattggctgt tttagcagtc gaagaaacag gcatgggtag agttgaagat 300 aaattggcta aaaatttggc ccaagctaac ggtactccag gtgtagaatg tttaagacca 360 gaagttttaa caggagatca tggtttgacc ttaatagaaa atgcagcctg gggtgttatt 420 gcttctgtaa ctccatcaac aaaccctgct gcaacagcca tcaataacgc tatctctatg 480 attgctggtg gtaattcagt catttttgca ccacaccctg ccgctaaaaa ggtttctcaa 540 agaacaatca ccatcttgaa tgaagctatt gttgcagccg gtggtccaaa taacttgtta 600 gtcactgtag ccaaacctga tatcgaaaca gctcaaagat tattcaagta tccaggtata 660 ggtttgttag ttgtcactgg tggtgacgct gtagttgaat ccgcaagaaa gcatacaaat 720 aagagattga ttgctgcagg tgctggtaac ccacctgtcg tagttgatga aacagcagac 780 atcgaaagag ccgctaaagc cattgttcac ggtgctagtt tcgataacaa catcatctgt 840 gctgacgaaa aagttttgat cgcagtcgat tgcattgccg acaagttgat cacagaaatg 900 caaagaaacc atgcagtttt gttgaccaga gaacaatctg aaaagttgat ccctgtattg 960 ttaaagaacg ttgatgaaac cggtcacggt actgtctcaa gagattgggt tggtagagat 1020 gctgccaaaa tagctgcagc catcggtatg actgttccag cagatacaag attgttaatt 1080 gccgaaaccg actgtaagca tccttttgct gtcactgaat tgatgatgcc agtattgcct 1140 atcataagag taaaggatgt tgaccaagca atagatttgg ccgttaagtt agaaggtggt 1200 tgtcatcaca ctgctgcaat gcactccaac aacatcagta atttgaacag aatggcaaac 1260 gccattgata catctatatt cgttaaaaac ggtccatgca tagctggttt gggtttaggt 1320 ggtgaaggtt ggactacaat gaccatcacc actcctactg gtgaaggtgt tacatgtgca 1380 agaacctttg tcagattaag aagatgcact atggttgatt cattcagaat tgtctga 1437 <210> 13 <211> 1434 <212> DNA <213> AADHpa <400> 13 atggaaatac aaaacttaga agaagatatc agaagaattt tatcagaaga attgaagaag 60 agtggtacct cacaaaccgc ctcaacctct gatgccggtc aaaatggtat attcaagact 120 gtagatgaag ctatcgcagc agctaaagct gcagaagatg tttacattga taaaacttta 180 gcctttagag aaaaggtctt gactgcaatt agagaaggtt tcagacctta tatagaaaag 240 atggcaaaag atatcaaaga tgaaactggt atgggtacag ttgaagcaaa aatcgctaag 300 ttgaataacg ccttgtacaa tacccctggt actgaaattt tgcaacctga agctgaaaca 360 ggtgacggtg gtttggttat gtatgaatat gctccttttg gtgttatcgg tgcagttggt 420 cctagtacaa atcctagtga aacagttatc gccaacgcta ttatgatgtt ggccggtggt 480 aatacattat attttggtgc acacccaggt gccaagaaaa tcactagatg gaccatagaa 540 aaattgaacg aattggttta tgaagctacc ggtatgaaaa acttagttgt ctccatcgaa 600 gaaccatcca tcgaatccgt tcaagaaatg atgcaacatc cagatatcgc tatgttgtca 660 atcacaggtg gtccagcagt cgttcatcaa gccttggttt caggtaaaaa ggccgttggt 720 gcaggtgccg gtaatccacc tgctattgtt gatgctacag caaatgttgc tttggcagcc 780 cataacattg ttgattcagc ctcctttgac aataacatat tgtgcacagc tgaaaaggaa 840 gttgtcgttg aaagttccgt taaagacgaa ttgattaaga aaatgcaaga agaaggtgct 900 ttcttggtta ctaatgcatc cgacattgat aagttggccg aaatgacaat cggtaaaaat 960 ggtgcacctg acagacaatt tgtcggtaaa gatgcaacct atatattgga caaggcaggt 1020 attgcttaca ctggtacacc aaagttaatc attttggaag cccaaaaaga ccatcctttg 1080 gttactacag aaatgttgat gcctattgtt ccagtcgtat cctgtcctac atttgaccaa 1140 gtcttagcaa cagccgtaga agttgaacaa ggtttgcatc acacagcatc aatccactcc 1200 gaaaacttgc ctaatatcaa tagagcagcc cacagaatga atacatctat cttcgtcgtt 1260 aacggtgcca catacgttgg tacaggtgtt ggtgctaacg gtgcacatgc tggtgcatct 1320 gcattgacta tcgccacacc aacaggtgaa ggtacagcaa ccgctaaaac ctttacaaga 1380 agaagaagat tgaacagtcc agaagccttt agtttgagaa gttgggaagc ctga 1434 <210> 14 <211> 1434 <212> DNA <213> AADHpb <400> 14 atgcaaacta cagaagccac tatcagaaac gtcgtccaag aagtattagc acaattagcc 60 aagcaatcaa atggttcaaa cggtgttcca agagcagaaa gaaaagccgg tgactggggt 120 gttttcgatg acactgacca agccattgct gcagccaatg aaggttttag agctttatct 180 tctaagggta tggaagaaag agcaaagatc gtagaaatcg ttaagagaat ctgtgctgat 240 caagcagaag aattgggtag attggaattt caagaaacca aagtcggtag attggaccat 300 aagatcgaaa agttgcaaat tattaagaaa gtaccaggta cagaatggtt gagaaccgaa 360 gctatgtccg gagatcatgg tttgacagtt attgaatatg caccttttgg tgtaatcggt 420 acaattaccc cagttactca cagtttgcct acattagctg gtaatgttat aaacatggtc 480 gctgcaggta atactttggt tgtcaaccca catccttctg gtaaaaagat tgcctgtgaa 540 ggtgttagaa gattcaatag agctatatac gccgctacag gtttggaaaa tgtaataacc 600 atcgtttcca acccaacttt agaaagtgct gatagaattt ttaaccatag agatgttaag 660 atgttatgcg taactggtgg tccaggtgtt gctagagcag ctatggcttc tagaaagaaa 720 gccgtagttg ctggtcctgg taatccacct gttttggtcg atgaatccgc ttgtttacaa 780 aacgctgcag aaagtatcat tgccggtgct ggttatgaca acaacttgtt gtgcataggt 840 gaaaaggaag tattcgtcgt agaatccgtt tttgatagat tcatgcaaga aatggacaag 900 gcaggtgccg ttagattgaa tgcatctcaa gtcgatcaat tatcaaaagt agcctttaca 960 ccacctgaaa acgaaaagga tcattaccac gttaacagag acttggtcgg tcaacatcca 1020 tacgttttag ctaaacaaat cggtttgcca ttacctgaat caacaccatt gttatttggt 1080 gaaaccgatg aacacaatcc tttcgtcgct gaagaacaaa tgatgccatt tgtccctata 1140 gtaagatgta gaaacgcaga acatggtatg gatttgtgcg aaaagtatga acatggtttc 1200 agacacactt ctttaatcca ctcaagaaac gttagaaaca tgtctatcat gggtaaaaga 1260 atggatacta cattgtttat caagaatggt ccatcacctg ccggtttggg tttaggtggt 1320 gaaggttact tatccttcag tattgcaact ccaacaggtg aaggtgttac cactcctttg 1380 acctttacta gacaaagaag atgtacaatg gttgatgact taagaattgt ctga 1434 <210> 15 <211> 1359 <212> DNA <213> AADHpe <400> 15 atggaaatca aagaaatggt cgccttagcc agaaaagccc aaaaagaata tcaagcaaca 60 cataatcaac aatcagtaga caacatatgt agagctgcag ccaaagttat atatgaaaac 120 gctgaaatgt tggcaagaga agccgttgac gaaactggta tgggtgttta tgaagataaa 180 gtctccaaga atagaggtaa atctaagggt gtttggtaca acttgcatga caaaaagtct 240 gtcggtatat taaacattga tgaaagaacc ggtatgatcg aaattgccaa acctatgggt 300 gttgtcggtg ctgtcacccc aactacaaat cctatagtaa ctccaatgtc taacatcatc 360 ttcgccttga aaacttgtaa cgctatcatc atctcaccac atcctagatc taaaaagtgc 420 tcaaaacacg cagttgattt gatcttagaa gccattaagc ctttcaacgt cccagagggt 480 atggttcaag tcatagaaga accttccatc gaaatgacac aacaattaat gggtgcagta 540 gatgtagttg tcgcaaccgg tggtatggct atggttaaat ctgcttattc ttcaggtaaa 600 ccatcatacg gtgttggtgc tggtaacgta caagttattt tcgattctga catagatttc 660 gaagatgctg cagaaaaagt tatatatggt agatcctacg ataatggtat catatgtact 720 ggtgaacaat ctgttatata taacgaagcc gacaaagatg ctgtttttgc cgctttaaga 780 aagagaggtg catacatttg cacagaagaa gaaggtaaaa agatgagaga cttgttgttc 840 caaaatggtg gtcctattaa caaagatgta gttggtcaag gtgtccaaaa gattgctaag 900 atggcaggta ttaatgtacc agaaggttct agagtcataa tcgtagaagc aaacggtgta 960 ggtgaagaag atgttatctg tagagaaaag atgtgtccag ttatgtgcgc tttgtcatac 1020 aagcatttcg aagaaggtgt tgatatcgca tgtagaaatt tggccaacga gggtaatggt 1080 cattcctgcg ctatacacag taacaaccaa gcacatatca tcttgactgg ttctaaagct 1140 acagtttcaa gaattgtcgt aaatgcccct tcagctacca ctgcaggtgg tcacatacaa 1200 aacggtttga atgttacaaa caccttaggt tgcggttcct ggggtaacaa ctccatcagt 1260 gaaaacttca catacaagca cttgatgaac attagtagaa tagctccatt gaaccctgct 1320 attaaagttc caaacgatca agaaatctgg gaattatga 1359 <210> 16 <211> 1383 <212> DNA <213> AADHrw <400> 16 atgaccgtta ccgctacatt ggacgcagat ttggcagcat tggcagacgt tagagcacaa 60 gtatccgcaa ctaagaaggc atttgacgcc atcgaaggtg ctgatcaaca atggttggac 120 gaaattgtta gagctatggc taaagcaggt tatgatgctt ctgaagaatt agccagaatg 180 gctgtagacg aaacaggtta tggtgtttac gaaaataaga ttattaaaaa tagattcaat 240 accggtttcg ttgctgattg gatgttggaa agaagagccg taggtgtttt atgggctgac 300 gaagaaaaca aggttactgc agtcggttct ccaatgggtg tcatagctgc aatcattcca 360 gtaacaaatc ctactgccac agttttgttt aaatccttgg caagtgttaa ggctggtaac 420 gccgttgtcc atgctccaca ccctagagcc gctagatgtt ctgcaagagc agccgaaatt 480 atggccgaag ctgcagtcca tgccggtgct ccagaaaatt tggtttcatg cttaagacaa 540 cctacattgg atgctaccag agaattaatg agacacggtg acattagatt ggttttagct 600 actggtggtc cagcaatggt tgccgctgca tattcttcag gtaaaccaac catagctgtt 660 ggtgctggta atgtccctgc ttacgtagat tccactgtta aagacttggc cgaaaccgct 720 actatgatag ttacatccaa gagtttcgat aacggtaccg catgtgttgc cgaacaaagt 780 gtagttatcg ccgctccagt tgctgatcaa ttggtccaag aattaggtag agcaggtgcc 840 acctggatga ctaaagatca acaagcagcc ttgacaagaa ccttatttga cgaaagaggt 900 ggtttgagag ctaatgcagt aggtcaatct gcagttagat tagccgaaat gtccggtttc 960 actgtccctg ctagtacaaa ggtattggct gcagaattag atgaagtcgg tgaccatgta 1020 ttgttatcaa gagaaatctt gggtccagta ttgactgttt acagagaacc tgattaccaa 1080 gcaggttgga gaagatgtag agaaattttg gctttaggtg gtgaaggtca taccgtcgca 1140 gtacacactt ccgatccaga agtcgtagct aaatttggtg aattgccagc aggtagaatt 1200 gttgtcaata ctcctgcttt atttggtggt atgggttact ctacaaacgt tgatccttca 1260 ttccaaattg gtactggtac atggtctggt tcaatatgct cagacaatgt tacaccattg 1320 catttggtca acataaagag aatcgctcac gaagcaagac cttggagagg tgttttcgat 1380 tga 1383 <210> 17 <211> 2616 <212> DNA <213> AADHsl <400> 17 atggctattg aagaacactc attgaacgac aaggaatcca agaaagaaac cttagaagaa 60 accattgact tattagtaac aaacgctaaa acagcttctg aagagtttac tccattggat 120 caacaacaaa tagacgaaat cgttagacaa atggcattgg ccggtttaga tcaacatatg 180 caattggcaa agatggccgt cgaagaaaca aagagaggtg tatacgaaga taagatgatt 240 aagaatatat ttgctacaga atacatctat aactctatta agaaaaataa gaccgtaggt 300 gttatttcag aagatccata cgaagaaatc gttcaaattg cagaacctat aggtgttatt 360 gctggtgtca cccctgtaac taacccaaca tctactacaa tgttcaagtc cttgattagt 420 ataaagacta aaaaccctat aatctttgcc ttccatccat ccgctcaaaa gtgtagtgct 480 gcagccgcta aagttgtcag agatgcagcc gttaaggctg gtgctcctaa aaattgcatt 540 caatggatag aaactccttc attggaagca acacaaagat taatgcatca cccaggtgta 600 tctttgattt tagctacagg tggttcaggc atggttaagg ctgcatattc ttcaggtaaa 660 cctgcaattg gtgtaggtcc tggtaatgtt ccagcctaca tagaaaagtc tgcagatgtt 720 aaacaagccg tcaacgactt gatcttgtcc aaaactttcg ataacggtat gatctgtgct 780 agtgaacaag cagtcatcgt agaccaacaa atctatgaag ctgttaaaaa ggaaatgatt 840 gataataagt gttacttctt gaacaacgaa gaaaagaaga aagttgaaaa gttagttatt 900 gatgaaagaa gatgcgcagt caacccacaa attgtaggta aaactgctgc ttttattgct 960 ggtttggcag gtgttacagt ccctgaagat accaaagtaa ttgttgctga attgtccagt 1020 gttggtccag aatctccttt atcaagagaa aaattgtcac cagtcttagc ctgttttaga 1080 gctaagacca ctgaagaagg tttcaaaaga tgcgaagaaa tgttagcatt tggtggtatt 1140 ggtcattctg ccgtaataca ctcaaacgat caaaaagtta tagacgcctt cgctatgaga 1200 atgaaggctg gtagattgat cgttaatgca ccatcttcat taggtggtat cggtgacatc 1260 tataaccatt ttattccttc cttgacctta ggttgtggta gttacggtgg taattccgtc 1320 agtaccaacg taggtactgc taatttgatc aacatcaaga ctttggcaaa cagaagaaac 1380 aacatgcaat ggttcaaggt tccacctaaa atctatttcg aaaagaacgc taccaaatac 1440 ttgtctgtta tgccagatat aactagagct tttattgtag ccgaccctgg tatggttaaa 1500 ttcggttatg ttgacagagt cattcatttc ttgaagcaaa gaagagatcc agttcactac 1560 gaagttttct ctgatgttga accagaccct tcagtagcta ctgttatgaa gggtacagaa 1620 ttgatgagaa aatttgaacc agacgttatc attgctttag gtggtggttc tgcaatggat 1680 gcagccaagg gcatgtggtt gttctatgaa tcacctgaaa cagatttctt tggtttgaag 1740 caaaagttct tggacataag aaagagagtc tttaaattcc cagatttggg taaaaaggct 1800 aaattcgtag caatccctac aacctccggt accggtagtg aagttacttc tttttcagtc 1860 ataactgata aggaaaacga catgaagtac ccattggccg actacgcttt aactcctaac 1920 gttgcaataa ttgatccaga atttgtcttg acagtacctc aaagagtctt agctgattcc 1980 ggtatggacg tattgactca tgcaattgaa gcctacgttt ctaacatggc taacgatttc 2040 acagacggtt tatgtatcaa ggcaatccaa ttggttttta aatatttgcc aagatcttac 2100 aaggattcaa ccgacagaga agccaaggaa aagatgcaca atgcttccac tatcgctggt 2160 atggcttttt ctaatgcctt cttgggtatt aaccattctt tggctcacaa attgggtaat 2220 gaatttcatt tggcccacgg tagatgttgc gctatcttgt tgcctcatgt tataagattc 2280 aacgctcaaa agccaacaaa atttgctgca ttccctaagt acaagcactt tattgctgat 2340 gaaagatacg ccgaaatagc tcaagttttg ggtatcgcag gtaaaactac agaagaatct 2400 gttgaaaatt tggtccaagc tattaagaaa ttgactcaag aattaaacat gccaatgtcc 2460 atcgaaaaga acggtgttag taaagaagct ttcgaagccg ctgtcgataa attagcagac 2520 agagcctttg gtgaccaatg cacaccagca aaccctaaga tgccattggt ttcagaatta 2580 accgaaatct atagacaagc ttttaaaggt atttga 2616 <210> 18 <211> 1389 <212> DNA <213> AADHss <400> 18 atggcagatg aacaattagt ccaaaatgta gtaagagaag tagtagcaag aatgcaaata 60 tccgctccag caagaggtat gcacggtgtt ttctctgata tggaagaagc aattgaagct 120 gcaagaactg cccaacaaac agttagattg ttaccaatgg accaaagaga aaagattatc 180 ggtgccataa gaagaaaaac tagagaaaac gctgaaatct tggcaagaat ggccgtaaat 240 gaaacaggca tgggtaacgt tggtgacaaa attttgaagc atttgttagt cgccgataaa 300 gtaccaggta ccgaagatat ctctactaga gctttttcag gtgacagagg tttgacttta 360 attgaaatgg gtcctttcgg tgtaatcggt gctattaccc catgtactaa tccttccgaa 420 acagttttat gcaacaccat cggcatgttg gcaggtggta atacagttgt ctttaaccca 480 catcctcaag ccataaagac tacattattc accatccaaa tggttaatga agcttcattg 540 gaagcaggtg gtccagataa catagcttgt acagttgacg cccctacatt agctacctct 600 gaaatcatga tgaagtcacc acacatcaag ttgttagtcg caaccggtgg tcctggtgta 660 gttactgctg tattatcttc aggtaaaaga gcaattggtg ccggtgctgg taatccacct 720 gccttggtcg atgaaaccgc tgacatcaga aaggccgctg aagatatagt taacggttgt 780 actttcgaca acaacttacc atgtattgct gaaaaggaaa ttgtcgcagt agattctatt 840 gccgacgaat tgttgcatta catgttgact gaacaaggtt gttaccaagc ttcagaagaa 900 gaattggata gattaacaaa ggcagttatg gacgaaaagg gtagattgaa tagaaaagct 960 gtcggtagat ccgcaagaaa gttgttaagt atgatcggtg ttgaagtcga tgctaacata 1020 agatgcatca ctttctttgg tccaaaggaa caccctttga taaccactga attaatgatg 1080 ccaatcttgg gtatcgtaag agttaaggat tttgcagaag gtttagaaac agcagcctgg 1140 ttggaacatg gtaataagca ctccgctcat atccacagta aaaacgttga tagaatcaca 1200 gaatacgcaa gaagattaga cacaaccatt accgttaaga atggtccttc ttatgctgca 1260 ttgggttttg gtggtgaatc ttactgtact ttcacaatag catcaagaac tggtgaaggt 1320 ttaacatccg ccagaagttt catcaaatcc agacattgtg ttatgactga tagtttgtgc 1380 gtcagatga 1389 <210> 19 <211> 1404 <212> DNA <213> AADHta <400> 19 atgaataaca ctgaattaga atccttgata agaaccattt tgaccgaaca attaacacca 60 tccgctactg atacccctgc ctgcactgcc tcttcagtcg ctttatttga tgacgtagat 120 tctgctattt gtgctgcaca tgccgctttc ttaagatatc aagaagcacc attgaaaact 180 agatctgcca ttatagcagc cattagagca gaaatagccc cttgcttatc agaattggca 240 gaaagagctg cagccgaaac tggtatgggt aacacagctg ataagatctt gaaaaataag 300 gctgcattgg aaaacacacc aggtattgaa gatttgaaaa ctacagcttt gaccggtgac 360 gaaggcatgg ttttgttcga atactctcct ttcggtgttg tcggtgcagt cgccccatca 420 acaaacccta ctgaaacaat cattaataac tccatcagta tgttggccgc tggtaatgct 480 atctattttt ccccacatcc tggtgcaaag aacatcagtt tgtggttgat ccaaaagatg 540 gaagaaatcg cttttaaagt ttgtggtatc cataacttga ttgtcaccgt aaaggaacca 600 acattcgaag ctacccaaca aatgatggca cacgataaaa ttgctttgtt agcaataact 660 ggtggtcctg gtatagttaa catgggttta aagtctggta aaaaggtcat aggtgctggt 720 gcaggtaacc caccttgctt ggttgacgaa actgcagaaa ttgtcaaggc agcccaagat 780 atagtagccg gtgcttcctt tgactacaat ttgccatgta ttgctgaaaa atctgttatt 840 gctgttgatt gcattgccga ccaattgata caacaaatga gagaatttgg tgccatgcaa 900 atcacagatc cacaacaaat tgctcaatta agagaagtct gtattcaaaa aggtgctgca 960 aataagtcct tagtaggtaa aagtccagct accatattgg ccgctgcagg tatcccatgc 1020 cctgcaaagg aacctagatt aataatcttg gaagttccag ctaacgatcc tttcgtagtt 1080 actgaacaat taatgccagt tttgcctatt gttagagtcg ataacttcga acaaggtttg 1140 caattagctt tgaaagttga agatggtttg catcacactg caatgatgca ttctcaaaat 1200 gtttcaagat taaacaaggc cgctcacttg atgcaaacat ctatattcgt taaaaatggt 1260 ccatcatacg ccggtatcgg tgttggtgct gaaggtttta ccactttcac aatagcaacc 1320 cctactggtg aaggtacaac ctccgccaga acctttggta gattaagaag atgtgttttg 1380 actaacggtt tctcaattag atga 1404 <210> 20 <211> 1425 <212> DNA <213> AADHtl <400> 20 atggtagaca tagacttaaa gaacgtagaa caaatcgtca gaactgtatt agcctcaatg 60 caaactcaac ctcaagccgt ccaacctgct gcaggtagaa gagccgctcc aggtgttttt 120 actgaattgg atgacgccgt tgctgaagca tctacagcac aaagagccat tagaaatttg 180 gctaccagag ttaaagttgt cgaagcaata agaaaggcag ccgaagccca tgctagagaa 240 ttggctgaat tagcagtcgc cgaaacaggc atgggtagag tagaagataa attcgccaag 300 aacgtttcac aagctagaca cacaccaggt gttgaatgtt tggctcctac cgcattaact 360 ggtgacagag gtttgacctt aattgaaaat gctgcatggg gtgtcatagc ttccgtaaca 420 ccaagtacca accctgccgc taccattatt aacaacgcaa tttctatgat ctcagccggt 480 aatactgtag tttttgctcc acatcctgca gccaaaaagg tttctcaaag aactgtctca 540 ttgttaaacg aagcatccgt tgctgcaggt ggtccagata atgtcgtaac tacagttttg 600 aaccctgata ttgctacagc acaaagatta ttcagttatc caggtattca tttgttaaca 660 gttaccggtg gtgaagctgt tgtcgaagcc gctagaaaac acactgataa gagattgatc 720 gcagccggtg caggtaatcc acctgtagtt gtcgatgaaa cagccgactt agctagagct 780 gcaagagata tagtttgggg tgcttccttc gataacaaca tcatctgtgt tgacgaaaag 840 gaaatcatcg tagttgatag agtctgcgac gcattgaaaa ctgaaatggc aagacataag 900 gccgttgaat tgacaccaga tcaaggtaaa agattagttg acgtcttgtt gaactatgtt 960 ggtgctgatg gtagaggtac tgtatctaga gattgggttg gtagagatgc tgctaagatc 1020 gcagccgcta ttggtttgga agttccagct gataccagat tgttatacat tgaaactcca 1080 gctgaccacc ctttcgcagt cacagaatta atgatgccag taatacctgt tgttagagca 1140 ccagatgttg atgcagccat tgatttggca gtaaagttag aaggcaactg tagacatacc 1200 gctgcaatgc actctagaaa cattgatgct ttagacagaa tggccaacgc tataaacaca 1260 tcaatcttcg ttaagaatgg tccatgcttg gcaggtttag gtttcggtgg tgaaggttgg 1320 accactatga ctatttccac tcctacaggt gaaggtgtta cttctgctag aacatttgtc 1380 agattgagaa gatgcgtatt agttgatcat ttcagaattg tttga 1425 <210> 21 <211> 2739 <212> DNA <213> AADHtm <400> 21 atggatacag ccgtagccgt tcaaaaacaa gatattgcca tgtctgccgc cagtaagcct 60 aagtctgcct caggtcctcc tttcgatgtc caagcacatg tagatggttt agttgcaaga 120 gccattagag ctttagcaga atttcaagct ttggatcaag aacaagttga tagaatagta 180 gccaaggctt cagttgctgc attaaatcaa cacggtgttt tggcaaaatt agccgtcgaa 240 gaaacaggta gaggtttgtt tgaagataaa gcagtcaaga acatgttcgc ctgtgaaaac 300 gtaacatctg caatgaagag aatgaaaact gtaggtgtta ttcatagaga taacttgaac 360 ggtatagtag aaatagctga accagttggt gttgtcgccg ctgtaactcc tgttacaaac 420 ccaaccagta ctgcaatttt taaggccttg gtttgtttaa aaactagaaa ccctatcgtc 480 tttggtttcc atccacacgc tcaaaagtgc tctgcagaag ccgtcagaat tgtaagagat 540 gcagccgtcg ctgcaggtgc tccaaccaac tgtgcacaat ggatagaagt tccttcaatc 600 aaagctactg gtgcattaat gaatcaccca gatatctccg taattttggc caccggtggt 660 gacgctatgg ttaaggccgc ttattcatgc ggtaaacctg ctattggtgt tggtcctggt 720 aacgttccag catttgtcga aaagactgcc gatgttgcta gagcagccta tgacttggtc 780 ttatctaaat cattcgatta tggtatggtc tgtgcatcag aacaagctgc aattatagaa 840 gccccagtat acgaccaagc tatggcagaa atgcaaagat tacatgcaca cgttgccact 900 tctgatgaaa agagaaagtt ggaagaatta gcatttggtg tagttgccaa cagtgaaaat 960 tgcggttctg ctaagttgaa cccagcaatc gttggtaaat ccgctagttg gttggctaaa 1020 caagcaggtt tcagtgttcc tcaagataca tctatcttgt tagtagaagc agatcatgtt 1080 ggttatgacg aaccattgac cagagaaaag ttgtgtccta tcttggctgt tattagagcc 1140 agagatgctg atgacggtat cagattatct gaacaaatgg ttgaacaaga cggtttgggt 1200 cattcagcat gcattcactc cactgatgaa gaagtcatcg aaaagttcgg tagaagaatt 1260 aaagctgtaa gaatcataac taactctcca acatcattag gtggtatagg tgacatctat 1320 aatgctttcg ttccttcatt gacattaggt tgtggtacct acggtaaaaa ttctgtttct 1380 aacaacgttc aagctgtcaa cttgattaat gtcaagagaa tcggtcatag aaacaacaac 1440 atacaatggt ttaaagttcc tgctaagatc tatttcgaac caaacgccat cagatactta 1500 gctgacatga gaagagtaaa cagagttttt atcgtcaccg atgctactat gtcaaaattg 1560 ggtgtcgtag atagaattag agatgttttg aacagaagat taaacatggt taccatgcaa 1620 gttgtcgata ctatagactc tgaaccatcc atcagtatgg tcgaagtagg tgctgaacaa 1680 atgagaaatt tccaacctga cacaattgtt gctttaggtg gtggttctgc aatggatgcc 1740 gctaaggtca tgtggttgtt atatgaacac ccagaagtct ctttttcaga tgtacgtgaa 1800 aagtgtttcg acgttagaaa aagagctttt actttccctg atttgggtga aaaagcacaa 1860 ttggtatgca tatccactac aagtggtact ggttctgaag ttacaccttt tgcttgcatc 1920 accgatccaa aaactggtta taagtaccaa ttgacagact acgccttaat gcctaccgta 1980 gctattgttg atccaacttt agttaacgct atgccttcaa gattggcagc cgattccggt 2040 atggacgcct tagctagagc aactgaagca tatgtttcag tctacgccaa cgattttaca 2100 gacggtttgg caatgcaagc cattaaattg atcttcgaaa atttgccagc atccgttttg 2160 aaaggtgacg ctaaggctag agaaaagatg cataatgctg gtacaatagc tggtatggca 2220 tccggtagtg catttttggg tatagttcat gcaatcacac acaccattgg tgcctcctat 2280 agaattagtc aaggtagatc taacgctact ttgttgccat tgggtattag atacaacggt 2340 agaataccaa caaagttgac ctcttggcct aaatatgaaa agtacgttgc tccacaaaga 2400 taccaagata ttgcaaagat gttgggttta cctgctgcaa ctccagaaga aggtgtcgaa 2460 agttatgcaa gagccgtaga agatttgaga gctacattga acatggaata ctcttttaga 2520 gcacaaggta tagaagaaca tgatttcgtt gctaacttag acgaaatggc cttcagagct 2580 tcagatgacc aatccgctcc agcaaatcct agattaccaa tggttgaaga tttgaagatc 2640 atcatggaag ccgcttatta cggtatcagt ttcgatgaca ttgtagccag aagagcctct 2700 gctcaattgg ctaaatctga agtttctaag aaaggttga 2739 <210> 22 <211> 1599 <212> DNA <213> AADHvs <400> 22 atgaacgaac aagaaatcgc ccacgcagta gaaaatgtat tatcaaagta caccaacgta 60 accgcacaaa acgccgaacc agtaagttat tcttcaaacg ctagtttgga aaacattgta 120 tctcaagcct tagctggtaa catggttaaa caaccagaaa cacaaaccgc acctgatttg 180 aactctaaca tcgaaaacat agtttcccaa atcttggccg aaaaccaagc taagccacaa 240 tcagttcaat gtcaatccgc taatcatggt actacagaat atttgggttg ctttgcctct 300 atggaagaag ctatttctgc tgcatcacat gcacaagttc aatacagaca ctgtactatg 360 ggtgacagag cctcatttgt caaaggtata agagaagtat tcactcaaga tgacgttttg 420 gaaaagatct ccagaatggc agttgaagaa accggtatgg gtaattacgc cgataaatta 480 actaagaaca gaatagccgc tactaaaaca ccaggtatcg aagatttgac cacttccgct 540 ttaagtggtg actctggttt gaccttaact gaattttctg catatggtgt tatcggttct 600 attacaccaa caaccaaccc tacagaaacc attattaaca actcaatcgg catgttggca 660 gccggtaata ctgttgtcta ctccccacat cctagaagta gaaatgtttc tttggttgct 720 gttgatttga ttaatagaaa gttggcagaa ttaggtgcac cagccaattt ggtagttact 780 gttttagaac cttcaattga taacacaaac gctatgatga acgacccaag agtaaacatg 840 ttagttgcaa caggtggtcc ttcaatcgtc aagactgtaa tgtccacagg taaaaaggct 900 attggtgctg gtgcaggtaa tccacctgca gtcgtagatg aaactgccaa catagaaaag 960 gctgcaaagg atatcattaa tggttgtgcc tttgacaata acttgccatg tattgctgaa 1020 aaagaagtca ttgttgtcaa tgaagtagcc gattatttga tacactgtat gaaaaagtca 1080 ggtgcttact tgttatgcga caagcaaaag atacaacaat tgcaaagttt ggttttgaac 1140 gaaaaaggta ccggtccaaa cacttctttt gtcggtaaag gtgctagata catcttggat 1200 aagttgaaca tccaagtttc tgatgacatt aaggtcatat tgatcgaaac agaaagaaat 1260 catcctttcg tagttcacga attgatgatg ccaatattac ctgtcgtaag agttgaaaat 1320 gtcgatgaag caatagactt agccatcaaa gttgaacatg gtaacagaca cacagctata 1380 atgcatagta ccaacgttga aaagttgtct aagatggcaa gattaatcca aactacaatc 1440 ttcgtcaaga atggtccatc ctatagtggt attggtgtag gtggtgaagg tcacaccact 1500 tttacaattg ctggtcctac cggtgaaggt ataacttcag ctagatcctt cgcaagatac 1560 agaagatgtg taatggttga agcattaaat attagatga 1599 <210> 23 <211> 2676 <212> DNA <213> ADHEec <400> 23 atggcagtca caaatgtagc cgaattgaac gccttagtag aaagagtcaa gaaagcccaa 60 agagaatacg caagttttac acaagaacaa gttgataaaa tattcagagc cgctgcattg 120 gccgcagccg acgcaagaat ccctttggca aagatggctg tagcagaaag tggtatgggt 180 attgttgaag ataaagttat caaaaaccat tttgcttccg aatatatcta caatgcctac 240 aaagatgaaa agacctgcgg tgttttatca gaagatgaca catttggtac catcactatt 300 gcagaaccaa ttggtattat ctgcggtatt gttccaacca caaacccaac atcaactgcc 360 atcttcaaat cattaatatc cttaaaaact agaaatgcaa ttatcttttc acctcaccca 420 agagccaaag atgctaccaa taaggctgca gacatcgttt tgcaagcagc catagccgca 480 ggtgcaccta aggacttaat tggttggata gaccaacctt cagttgaatt atcaaacgca 540 ttgatgcatc acccagacat aaacttgatc ttagcaacag gtggtcctgg tatggttaag 600 gcagcctaca gttccggtaa accagcaatc ggtgttggtg ctggtaatac accagtagtc 660 atagacgaaa ctgctgatat caagagagct gtagcctcag ttttaatgtc caagacattt 720 gataatggtg ttatctgtgc atctgaacaa tccgttgtag ttgttgattc agtctatgat 780 gccgttagag aaagattcgc cactcatggt ggttacttat tgcaaggtaa agaattaaag 840 gctgttcaag atgttatctt gaagaacggt gctttgaatg ctgcaatagt aggtcaacct 900 gcttataaaa tagccgaatt ggcaggtttt tccgtacctg aaaacacaaa aatcttgatt 960 ggtgaagtaa cagtcgttga tgaaagtgaa ccttttgcac atgaaaaatt gagtcctact 1020 ttggcaatgt atagagccaa agatttcgaa gatgcagtcg aaaaggctga aaaattagtt 1080 gcaatgggtg gtatcggtca tacatcatgc ttgtacacag accaagataa tcaacctgct 1140 agagtaagtt actttggtca aaagatgaaa acagccagaa tattgattaa taccccagca 1200 tcacaaggtg gtattggtga cttatataac tttaaattgg caccatcatt gaccttaggt 1260 tgcggttcct ggggtggtaa ctcaatctcc gaaaacgtcg gtcctaaaca cttgataaac 1320 aaaaagactg ttgcaaaaag agcagaaaac atgttatggc ataaattgcc aaagagtatc 1380 tacttcagaa gaggttcctt accaatcgcc ttggatgaag ttattactga cggtcataag 1440 agagcattaa tcgttacaga cagatttttg ttcaacaacg gttatgctga ccaaatcaca 1500 tcagtattga aagccgctgg tgttgaaact gaagtctttt tcgaagttga agctgatcct 1560 acattgagta tcgttagaaa gggtgctgaa ttagctaatt cttttaaacc tgacgttatt 1620 atcgcattgg gtggtggtag tccaatggat gccgcaaaaa tcatgtgggt catgtatgaa 1680 cacccagaaa cacacttcga agaattagca ttgagattca tggacatcag aaaaagaatc 1740 tacaaattcc caaagatggg tgtaaaggcc aaaatgatcg cagttacaac aacatcaggt 1800 accggttccg aagtaacccc atttgcagtt gtcacagatg atgccactgg tcaaaaatac 1860 ccattagctg attatgcttt gacacctgat atggccatcg ttgacgctaa cttggttatg 1920 gacatgccta aatcattatg cgcttttggt ggtttggatg cagttaccca tgcaatggaa 1980 gcttatgttt cagttttagc ttccgaattt tctgatggtc aagctttaca agccttaaaa 2040 ttattgaaag aatacttgcc agcatcatat catgaaggtt ccaagaatcc tgttgccaga 2100 gaaagagtac attcagccgc aactatagcc ggtattgcat tcgctaatgc atttttaggt 2160 gtttgtcatt caatggccca caagttgggt tctcaatttc atataccaca tggtttggca 2220 aatgccttat taatttgtaa cgttatcaga tataacgcca atgacaatcc aactaaacaa 2280 acagcatttt cccaatacga cagaccacaa gcaagaagaa gatacgcaga aatcgcagat 2340 catttgggtt tgtctgcacc tggtgacaga accgcagcta agattgaaaa gttattagca 2400 tggttggaaa ccttaaaggc agaattgggt atcccaaagt ctattagaga agcaggtgtc 2460 caagaagcag atttcttagc caatgtagac aagttgtccg aagatgcatt cgatgaccaa 2520 tgcacaggtg caaatccaag atatccattg atctccgaat taaaacaaat attattggac 2580 acatactatg gtagagatta tgtagaaggt gaaaccgcag ccaagaaaga agcagctcca 2640 gctaaggcag aaaagaaggc taaaaagtcc gcttaa 2676 <210> 24 <211> 2658 <212> DNA <213> AHEpm <400> 24 atgtctggtt tacaaatgtt ccaaaacttg tccttatacg gttctttagc cgaaatagat 60 acctctgaaa aattgaatga agctatggac aaattgacag ccgcacaaga acaattcaga 120 gaatataacc aagaacaagt tgataagatc tttaaggccg tcgcattggc agcttcacaa 180 aacagagttg ctttcgcaaa atacgcacac gaagaaactc aaaagggtgt ttttgaagat 240 aaagttatca aaaacgaatt tgcagctgat tatatctacc ataaatattg taacgacaag 300 actgcaggta ttatcgaata tgatgaagca aatggtttaa tggaaatcgc agaaccagtt 360 ggtcctgttg ttggtatcgc acctgttaca aacccaacat ccactatcat atataagtca 420 ttaatcgcat taaagacaag aaattgcatt atattctccc cacatcctgg tgcccataaa 480 gcttcagttt ttgtagtcaa ggtcttgcac caagcagccg taaaagctgg tgcaccagaa 540 aactgcatcc aaataatctt tccaaagatg gacttgacta cagaattgtt acaccatcaa 600 aagaccagat tcatttgggc aaccggtggt ccaggtttgg ttcatgcctc ctatacttcc 660 ggtaaaccag ctttaggtgg tggtcctggt aatgctccag cattgattga tgaaacttgc 720 gatatgaatg aagccgtagg ttctatcgtc gtttctaaaa cattcgactg tggtatgatc 780 tgcgcaacag aaaacgcagt tgttgtagtt gaaagtgttt acgaaaactt tgttgcaact 840 atgaagaaaa gaggtgcata tttcatgact ccagaagaaa ccaagaaagc atcaaacttg 900 ttattcggtg aaggtatgag attgaacgct aaggcagttg gtcaaaccgc taaaactttg 960 gctgaaatgg ccggtttcga agttcctgaa aacacagttg tcttgtgtgg tgaagcttcc 1020 gaagttaaat ttgaagaacc tatggctcat gaaaaattaa ctacaatctt aggtatatat 1080 aaggcaaagg attttgatga cggtgttaga ttgtgcaagg aattggtcac atttggtggt 1140 aaaggtcata cagcagtttt atatacaaat caaaataata aggatagaat cgaaaagtac 1200 caaaatgaag ttcctgcatt tcatatctta gttgatatgc cttcttcctt gggttgcatt 1260 ggtgacatgt ataactttag attagcacca gccttgacaa tcacatgtgg tactatgggt 1320 ggtggttcca gttccgacaa catcggtcca aagcacttgt taaatattaa aagagttggt 1380 atgagaagag aaaacatgtt atggttcaaa attcctaaga gtgtttactt caaaagagcc 1440 attttatctg aagctttatc agacttgaga gacacccata agagagcaat aatcatcacc 1500 gatagaacta tgactatgtt gggtcaaaca gacaagatca ttaaagcatg cgaaggtcac 1560 ggtatggtat gcactgtcta cgataaggtt gtcccagatc ctacaatcaa gtgtatcatg 1620 gaaggtgtta acgaaatgaa cgtattcaag ccagatttgg ccattgcctt gggtggtggt 1680 tcagctatgg atgcagcaaa aatgatgaga ttattttacg aatatccaga tcaagacttg 1740 caagatattg ccacaagatt cgttgacatc agaaagagag tagttggttg tcctaaattg 1800 ggtagattaa tcaagacttt agtctgtata cctactacat caggtaccgg tgcagaagtt 1860 accccatttg cagttgtcac atccgaagaa ggtagaaaat atcctttagt cgattatgaa 1920 ttaacccctg acatggctat tgttgatcca gaatttgccg ttggtatgcc aaagagattg 1980 acatcatgga ctggtatcga cgcattgact cacgcaatcg aatcctatgt cagtattatg 2040 gcaactgact ttacaagacc ttattcattg agagcagttg gtttaatttt tgaatccttg 2100 tcattagcct ataacaatgg taaagacatt gaagctagag aaaagatgca caatgcttcc 2160 gccattgccg gtatggcctt tgcaaatgca tttttgggtt gctgtcattc cgtagcccac 2220 caattgggtt ctgtttatca catccctcat ggtttggcca atgctttgat gttgtcccac 2280 ataatcaaat ataatgcaac cgactctcct gttaaaatgg gtacatttcc acaatacaaa 2340 taccctcaag caatgagaca ctatgctgaa atcgccgaat tattgttgcc acctacccaa 2400 gtcgtaaaaa tgactgatgt tgacaaagtt caatatttga ttgatagagt tgaacaattg 2460 aaagctgatg ttggtattcc taaatccatt aaggaaactg gtatggtcac cgaagaagat 2520 ttctttaaca aggttgatca agttgctatc atggccttcg acgatcaatg tacaggtgct 2580 aacccaagat acccattagt ctccgaatta aaacaattga tgatagatgc ctggaatggt 2640 gtagtcccta agttataa 2658 <210> 25 <211> 1380 <212> DNA <213> EUTEdz <400> 25 atggaacact ccgtcattga accaaccgtc cctatgcctt tgccagctat gtttgatgcc 60 ccttcaggta tcttctcctc cttagacgat gcagttcaag ccgctacttt agcccaacaa 120 caattgtcat ccgttgaatt gagacaacaa gtcataaagg caattagagt tgccggtgaa 180 agatacgctc aagtcttggc agaaatggcc gtcgcagaaa ccggtatggg tagagttgta 240 gataaataca ttaagaatgt ttcacaagca agacatacac caggtatcga atgtttatcc 300 gctgaagtat taacaggtga caacggtttg accttgattg aaaatgcacc ttggggtgtt 360 gttgcttccg ttacaccatc aaccaaccca gctgctacag ttattaacaa cgcaatttct 420 atgatcgctg ccggtaactc agttgtcttt gcacctcatc catctgcaaa gaatgtttcc 480 ttaagaacta tctccttgtt gaataaggca atagtagcca caggtggtcc agaaaatttg 540 ttagtctctg ttgcaaaccc taatatcgaa actgcacaaa gattgtttag atatccaggt 600 atcggtttgt tggttgtaac cggtggtgaa gctgtagtcg aagctgcaag aaagcatacc 660 gataaaagat taatcgctgc aggtgcaggt aatccaccag ttgttgttga cgaaacagct 720 gatatcccta aggcagcaag agccattgtt aaaggtgcat cattcgataa caatattatc 780 tgtgccgatg aaaaagtttt aattgtagtt gacagagttg ccgatgcatt attggccgaa 840 atgcaaagaa ataacgcagt attattgact ccagaacaaa cagaaagatt gttaccagct 900 ttgttatctg atatcgacga acaaggtaaa ggtagagtta acagagacta cgttggtaga 960 gatgccgcta aattagcagc tgcaatcggt ttggaagtct cagaacatac aagattattg 1020 ttggcagaaa cagatgcaga tcatccattc gctgttacag aattaatgat gccagtttta 1080 cctgtcatca gagtcaagaa cgttgatgac gcaattgctt tggcagtcaa attggaatct 1140 ggttgtagac atactgctgc tatgcattca accaacataa gaaatttgaa tagaatggcc 1200 aacgctataa atacatcaat attcgttaag aatggtcctt gtatcgctgg tttaggtttg 1260 ggtggtgaag gttggacaag tatgacaatc agtaccccaa ctggtgaagg tgttacatca 1320 gctagaacct ttgttagatt gagaagatgc gtcttagtag atatgtttag aatagcctaa 1380 1380 <210> 26 <211> 1404 <212> DNA <213> EUTEec <400> 26 atgaaccaac aagacataga acaagtagta aaagccgtat tattgaagat gcaaagttcc 60 gacactccac cagccgccgt ccacgaaatg ggtgtattcg catcattaga tgacgcagtt 120 gctgccgcta aagttgccca acaaggtttg aagtcagttg ctatgagaca attggcaatt 180 gctgcaatta gagaagctgg tgaaaaacat gccagagact tggccgaatt agctgtctca 240 gaaactggta tgggtagagt tgaagataaa tttgccaaaa acgttgcaca agctagaggt 300 actcctggtg tagaatgttt gtccccacaa gttttaacag gtgacaacgg tttgacctta 360 attgaaaatg ccccttgggg tgttgtagct tctgtcacac cttcaaccaa ccctgctgct 420 accttgatca ataacgctat ttccttgatt gctgccggta attccgttat cttcgctcca 480 catccagcag ccaagaaagt ttcacaaaga gccatcacct tgttgaatca agcaatcgtt 540 gctgcaggtg gtccagaaaa cttattggtt acagtcgcaa accctgacat cgaaacagct 600 caaagattgt ttaaatttcc tggtataggt ttgttagttg taactggtgg tgaagctgta 660 gttgaagcag ctagaaagca tactaataag agattgattg ctgcaggtgc cggtaatcct 720 ccagtcgttg ttgatgaaac agcagatttg gccagagctg cacaatccat cgttaagggt 780 gcatctttcg ataacaatat tatctgtgca gatgaaaagg tcttaatcgt tgtagattcc 840 gttgctgatg aattgatgag attgatggaa ggtcaacatg cagtcaaatt gacagctgaa 900 caagcccaac aattgcaacc tgtattattg aagaatatcg acgaaagagg taaaggtacc 960 gtttcaagag actgggttgg tagagatgct gcaaaaattg ctgcagctat cggtttaaac 1020 gttcctcaag aaaccagatt gttgtttgtt gaaactaccg cagaacaccc tttcgcagtt 1080 accgaattga tgatgcctgt attgccagta gtcagagttg ccaacgttgc tgatgcaata 1140 gccttggcag ttaaattgga aggtggttgt caccatacag cagctatgca ttcaagaaat 1200 atagaaaata tgaaccaaat ggctaacgca atagacacat ctatctttgt taagaacggt 1260 ccttgcatag ctggtttggg tttaggtggt gaaggttgga caaccatgac aatcactact 1320 ccaacaggtg aaggtgtcac atcagcaaga acctttgtta gattaagaag atgcgtcttg 1380 gtagatgcct ttagaatagt ataa 1404 <210> 27 <211> 1410 <212> DNA <213> LIN1129li <400> 27 atggaatcat tagaattaga acaattagtc aagaaggtat tattagaaaa gttggcagaa 60 caaaaggaag tccctacaaa gaccacaacc caaggtgcaa aatctggtgt attcgataca 120 gttgacgaag ccgtacaagc agctgttata gctcaaaatt gttacaagga aaaatccttg 180 gaagaaagaa gaaatgttgt aaaagcaata agagaagcct tatacccaga aattgaaact 240 atagctacaa gagcagttgc tgaaactggt atgggtaacg ttaccgataa aattttgaag 300 aataccttgg ctattgaaaa gacccctggt gtagaagatt tgtacacaga agtcgctaca 360 ggtgacaatg gtatgacctt gtatgaatta tcaccatacg gtgttattgg tgctgttgca 420 ccttctacca acccaaccga aacattaatt tgtaactcta ttggtatgtt agcagctggt 480 aatgccgtat tttattcacc acaccctggt gccaaaaata tctcattatg gttgattgaa 540 aagttgaata ccatagttag agattcctgt ggtatagata acttgatagt aacagtcgct 600 aaaccaagta tccaagctgc acaagaaatg atgaatcatc ctaaggttcc attattggtt 660 ataaccggtg gtccaggtgt tgtcttgcaa gcaatgcaat ccggtaaaaa ggttatcggt 720 gcaggtgcag gtaatcctcc aagtatagtt gacgaaactg ctaacataga aaaagcagcc 780 gcagacatcg ttgatggtgc ctcatttgat cataatatct tgtgtatagc tgaaaagagt 840 gtcgttgctg tagattccat tgccgatttc ttgttatttc aaatggaaaa gaatggtgca 900 ttgcatgtta ccaacccttc tgatattcaa aaattagaaa aggtagccgt tacagataag 960 ggtgtcacta ataagaaatt agttggtaaa tccgccactg aaatcttgaa ggaagcaggt 1020 atagcttgtg actttacacc aagattgata atcgttgaaa ccgaaaaatc ccatccattt 1080 gcaacagttg aattgttaat gcctatcgtt ccagttgtca gagttccaga tttcgatgaa 1140 gcattggaag ttgctattga attagaacaa ggtttgcatc acacagctac tatgcactct 1200 caaaatattt ccagattgaa taaggcagca agagatatgc aaacaagtat cttcgtcaaa 1260 aatggtcctt cttttgcagg tttgggtttc agaggtgaag gttcaaccac ttttacaatt 1320 gccactccaa ccggtgaagg tactaccaca gccagacatt tcgccagaag aagaagatgc 1380 gtcttgacag atggttttag tattagataa 1410 <210> 28 <211> 6702 <212> DNA <213> ACC1sc <400> 28 atgagcgaag aaagcttatt cgagtcttct ccacagaaga tggagtacga aattacaaac 60 tactcagaaa gacatacaga acttccaggt catttcattg gcctcaatac agtagataaa 120 ctagaggagt ccccgttaag ggactttgtt aagagtcacg gtggtcacac ggtcatatcc 180 aagatcctga tagcaaataa tggtattgcc gccgtgaaag aaattagatc cgtcagaaaa 240 tgggcatacg agacgttcgg cgatgacaga accgtccaat tcgtcgccat ggccacccca 300 gaagatctgg aggccaacgc agaatatatc cgtatggccg atcaatacat tgaagtgcca 360 ggtggtacta ataataacaa ctacgctaac gtagacttga tcgtagacat cgccgaaaga 420 gcagacgtag acgccgtatg ggctggctgg ggtcacgcct ccgagaatcc actattgcct 480 gaaaaattgt cccagtctaa gaggaaagtc atctttattg ggcctccagg taacgccatg 540 aggtctttag gtgataaaat ctcctctacc attgtcgctc aaagtgctaa agtcccatgt 600 attccatggt ctggtaccgg tgttgacacc gttcacgtgg acgagaaaac cggtctggtc 660 tctgtcgacg atgacatcta tcaaaagggt tgttgtacct ctcctgaaga tggtttacaa 720 aaggccaagc gtattggttt tcctgtcatg attaaggcat ccgaaggtgg tggtggtaaa 780 ggtatcagac aagttgaacg tgaagaagat ttcatcgctt tataccacca ggcagccaac 840 gaaattccag gctcccccat tttcatcatg aagttggccg gtagagcgcg tcacttggaa 900 gttcaactgc tagcagatca gtacggtaca aatatttcct tgttcggtag agactgttcc 960 gttcagagac gtcatcaaaa aattatcgaa gaagcaccag ttacaattgc caaggctgaa 1020 acatttcacg agatggaaaa ggctgccgtc agactgggga aactagtcgg ttatgtctct 1080 gccggtaccg tggagtatct atattctcat gatgatggaa aattctactt tttagaattg 1140 aacccaagat tacaagtcga gcatccaaca acggaaatgg tctccggtgt taacttacct 1200 gcagctcaat tacaaatcgc tatgggtatc cctatgcata gaataagtga cattagaact 1260 ttatatggta tgaatcctca ttctgcctca gaaatcgatt tcgaattcaa aactcaagat 1320 gccaccaaga aacaaagaag acctattcca aagggtcatt gtaccgcttg tcgtatcaca 1380 tcagaagatc caaacgatgg attcaagcca tcgggtggta ctttgcatga actaaacttc 1440 cgttcttcct ctaatgtttg gggttacttc tccgtgggta acaatggtaa tattcactcc 1500 ttttcggact ctcagttcgg ccatattttt gcttttggtg aaaatagaca agcttccagg 1560 aaacacatgg ttgttgccct gaaggaattg tccattaggg gtgatttcag aactactgtg 1620 gaatacttga tcaaactttt ggaaactgaa gatttcgagg ataacactat taccaccggt 1680 tggttggacg atttgattac tcataaaatg accgctgaaa agcctgatcc aactcttgcc 1740 gtcatttgcg gtgccgctac aaaggctttc ttagcatctg aagaagcccg ccacaagtat 1800 atcgaatcct tacaaaaggg acaagttcta tctaaagacc tactgcaaac tatgttccct 1860 gtagatttta tccatgaggg taaaagatac aagttcaccg tagctaaatc cggtaatgac 1920 cgttacacat tatttatcaa tggttctaaa tgtgatatca tactgcgtca actagctgat 1980 ggtggtcttt tgattgccat aggcggtaaa tcgcatacca tctattggaa agaagaagtt 2040 gctgctacaa gattatccgt tgactctatg actactttgt tggaagttga aaacgatcca 2100 acccagttgc gtactccatc ccctggtaaa ttggttaaat tcttggtgga aaatggtgaa 2160 cacattatca agggccaacc atatgcagaa attgaagtta tgaaaatgca aatgcctttg 2220 gtttctcaag aaaatggtat cgtccagtta ttaaagcaac ctggttctac cattgttgca 2280 ggtgatatca tggctattat gactcttgac gatccatcca aggtcaagca cgctctacca 2340 tttgaaggta tgctgccaga ttttggttct ccagttatcg aaggaaccaa acctgcctat 2400 aaattcaagt cattagtgtc tactttggaa aacattttga agggttatga caaccaagtt 2460 attatgaacg cttccttgca acaattgata gaggttttga gaaatccaaa actgccttac 2520 tcagaatgga aactacacat ctctgcttta cattcaagat tgcctgctaa gctagatgaa 2580 caaatggaag agttagttgc acgttctttg agacgtggtg ctgttttccc agctagacaa 2640 ttaagtaaat tgattgatat ggccgtgaag aatcctgaat acaaccccga caaattgctg 2700 ggcgccgtcg tggaaccatt ggcggatatt gctcataagt actctaacgg gttagaagcc 2760 catgaacatt ctatatttgt ccatttcttg gaagaatatt acgaagttga aaagttattc 2820 aatggtccaa atgttcgtga ggaaaatatc attctgaaat tgcgtgatga aaaccctaaa 2880 gatctagata aagttgcgct aactgttttg tctcattcga aagtttcagc gaagaataac 2940 ctgatcctag ctatcttgaa acattatcaa ccattgtgca agttatcttc taaagtttct 3000 gccattttct ctactcctct acaacatatt gttgaactag aatctaaggc taccgctaag 3060 gtcgctctac aagcaagaga aattttgatt caaggcgctt taccttcggt caaggaaaga 3120 actgaacaaa ttgaacatat cttaaaatcc tctgttgtga aggttgccta tggctcatcc 3180 aatccaaagc gctctgaacc agatttgaat atcttgaagg acttgatcga ttctaattac 3240 gttgtgttcg atgttttact tcaattccta acccatcaag acccagttgt gactgctgca 3300 gctgctcaag tctatattcg tcgtgcttat cgtgcttaca ccataggaga tattagagtt 3360 cacgaaggtg tcacagttcc aattgttgaa tggaaattcc aactaccttc agctgcgttc 3420 tccacctttc caactgttaa atctaaaatg ggtatgaaca gggctgtttc tgtttcagat 3480 ttgtcatatg ttgcaaacag tcagtcatct ccgttaagag aaggtatttt gatggctgtg 3540 gatcatttag atgatgttga tgaaattttg tcacaaagtt tggaagttat tcctcgtcac 3600 caatcttctt ctaacggacc tgctcctgat cgttctggta gctccgcatc gttgagtaat 3660 gttgctaatg tttgtgttgc ttctacagaa ggtttcgaat ctgaagagga aattttggta 3720 aggttgagag aaattttgga tttgaataag caggaattaa tcaatgcttc tatccgtcgt 3780 atcacattta tgttcggttt taaagatggg tcttatccaa agtattatac ttttaacggt 3840 ccaaattata acgaaaatga aacaattcgt cacattgagc cggctttggc cttccaactg 3900 gaattaggaa gattgtccaa cttcaacatt aaaccaattt tcactgataa tagaaacatc 3960 catgtctacg aagctgttag taagacttct ccattggata agagattctt tacaagaggt 4020 attattagaa cgggtcatat ccgtgatgac atttctattc aagaatatct gacttctgaa 4080 gctaacagat tgatgagtga tatattggat aatttagaag tcaccgacac ttcaaattct 4140 gatttgaatc atatcttcat caacttcatt gcggtgtttg atatctctcc agaagatgtc 4200 gaagccgcct tcggtggttt cttagaaaga tttggtaaga gattgttgag attgcgtgtt 4260 tcttctgccg aaattagaat catcatcaaa gatcctcaaa caggtgcccc agtaccattg 4320 cgtgccttga tcaataacgt ttctggttat gttatcaaaa cagaaatgta caccgaagtc 4380 aagaacgcaa aaggtgaatg ggtatttaag tctttgggta aacctggatc catgcattta 4440 agacctattg ctactcctta ccctgttaag gaatggttgc aaccaaaacg ttataaggca 4500 cacttgatgg gtaccacata tgtctatgac ttcccagaat tattccgcca agcatcgtca 4560 tcccaatgga aaaatttctc tgcagatgtt aagttaacag atgatttctt tatttccaac 4620 gagttgattg aagatgaaaa cggcgaatta actgaggtgg aaagagaacc tggtgccaac 4680 gctattggta tggttgcctt taagattact gtaaagactc ctgaatatcc aagaggccgt 4740 caatttgttg ttgttgctaa cgatatcaca ttcaagatcg gttcctttgg tccacaagaa 4800 gacgaattct tcaataaggt tactgaatat gctagaaagc gtggtatccc aagaatttac 4860 ttggctgcaa actcaggtgc cagaattggt atggctgaag agattgttcc actatttcaa 4920 gttgcatgga atgatgctgc caatccggac aagggcttcc aatacttata cttaacaagt 4980 gaaggtatgg aaactttaaa gaaatttgac aaagaaaatt ctgttctcac tgaacgtact 5040 gttataaacg gtgaagaaag atttgtcatc aagacaatta ttggttctga agatgggtta 5100 ggtgtcgaat gtctacgtgg atctggttta attgctggtg caacgtcaag ggcttaccac 5160 gatatcttca ctatcacctt agtcacttgt agatccgtcg gtatcggtgc ttatttggtt 5220 cgtttgggtc aaagagctat tcaggtcgaa ggccagccaa ttattttaac tggtgctcct 5280 gcaatcaaca aaatgctggg tagagaagtt tatacttcta acttacaatt gggtggtact 5340 caaatcatgt ataacaacgg tgtttcacat ttgactgctg ttgacgattt agctggtgta 5400 gagaagattg ttgaatggat gtcttatgtt ccagccaagc gtaatatgcc agttcctatc 5460 ttggaaacta aagacacatg ggatagacca gttgatttca ctccaactaa tgatgaaact 5520 tacgatgtaa gatggatgat tgaaggtcgt gagactgaaa gtggatttga atatggtttg 5580 tttgataaag ggtctttctt tgaaactttg tcaggatggg ccaaaggtgt tgtcgttggt 5640 agagcccgtc ttggtggtat tccactgggt gttattggtg ttgaaacaag aactgtcgag 5700 aacttgattc ctgctgatcc agctaatcca aatagtgctg aaacattaat tcaagaacct 5760 ggtcaagttt ggcatccaaa ctccgccttc aagactgctc aagctatcaa tgactttaac 5820 aacggtgaac aattgccaat gatgattttg gccaactgga gaggtttctc tggtggtcaa 5880 cgtgatatgt tcaacgaagt cttgaagtat ggttcgttta ttgttgacgc attggtggat 5940 tacaaacaac caattattat ctatatccca cctaccggtg aactaagagg tggttcatgg 6000 gttgttgtcg atccaactat caacgctgac caaatggaaa tgtatgccga cgtcaacgct 6060 agagctggtg ttttggaacc acaaggtatg gttggtatca agttccgtag agaaaaattg 6120 ctggacacca tgaacagatt ggatgacaag tacagagaat tgagatctca attatccaac 6180 aagagtttgg ctccagaagt acatcagcaa atatccaagc aattagctga tcgtgagaga 6240 gaactattgc caatttacgg acaaatcagt cttcaatttg ctgatttgca cgataggtct 6300 tcacgtatgg tggccaaggg tgttatttct aaggaactgg aatggaccga ggcacgtcgt 6360 ttcttcttct ggagattgag aagaagattg aacgaagaat atttgattaa aaggttgagc 6420 catcaggtag gcgaagcatc aagattagaa aagatcgcaa gaattagatc gtggtaccct 6480 gcttcagtgg accatgaaga tgataggcaa gtcgcaacat ggattgaaga aaactacaaa 6540 actttggacg ataaactaaa gggtttgaaa ttagagtcat tcgctcaaga cttagctaaa 6600 aagatcagaa gcgaccatga caatgctatt gatggattat ctgaagttat caagatgtta 6660 tctaccgatg ataaagaaaa attgttgaag actttgaaat aa 6702 <210> 29 <211> 6702 <212> DNA <213> ACC1sc_S659A/S1157A <400> 29 atgagcgaag aaagcttatt cgagtcttct ccacagaaga tggagtacga aattacaaac 60 tactcagaaa gacatacaga acttccaggt catttcattg gcctcaatac agtagataaa 120 ctagaggagt ccccgttaag ggactttgtt aagagtcacg gtggtcacac ggtcatatcc 180 aagatcctga tagcaaataa tggtattgcc gccgtgaaag aaattagatc cgtcagaaaa 240 tgggcatacg agacgttcgg cgatgacaga accgtccaat tcgtcgccat ggccacccca 300 gaagatctgg aggccaacgc agaatatatc cgtatggccg atcaatacat tgaagtgcca 360 ggtggtacta ataataacaa ctacgctaac gtagacttga tcgtagacat cgccgaaaga 420 gcagacgtag acgccgtatg ggctggctgg ggtcacgcct ccgagaatcc actattgcct 480 gaaaaattgt cccagtctaa gaggaaagtc atctttattg ggcctccagg taacgccatg 540 aggtctttag gtgataaaat ctcctctacc attgtcgctc aaagtgctaa agtcccatgt 600 attccatggt ctggtaccgg tgttgacacc gttcacgtgg acgagaaaac cggtctggtc 660 tctgtcgacg atgacatcta tcaaaagggt tgttgtacct ctcctgaaga tggtttacaa 720 aaggccaagc gtattggttt tcctgtcatg attaaggcat ccgaaggtgg tggtggtaaa 780 ggtatcagac aagttgaacg tgaagaagat ttcatcgctt tataccacca ggcagccaac 840 gaaattccag gctcccccat tttcatcatg aagttggccg gtagagcgcg tcacttggaa 900 gttcaactgc tagcagatca gtacggtaca aatatttcct tgttcggtag agactgttcc 960 gttcagagac gtcatcaaaa aattatcgaa gaagcaccag ttacaattgc caaggctgaa 1020 acatttcacg agatggaaaa ggctgccgtc agactgggga aactagtcgg ttatgtctct 1080 gccggtaccg tggagtatct atattctcat gatgatggaa aattctactt tttagaattg 1140 aacccaagat tacaagtcga gcatccaaca acggaaatgg tctccggtgt taacttacct 1200 gcagctcaat tacaaatcgc tatgggtatc cctatgcata gaataagtga cattagaact 1260 ttatatggta tgaatcctca ttctgcctca gaaatcgatt tcgaattcaa aactcaagat 1320 gccaccaaga aacaaagaag acctattcca aagggtcatt gtaccgcttg tcgtatcaca 1380 tcagaagatc caaacgatgg attcaagcca tcgggtggta ctttgcatga actaaacttc 1440 cgttcttcct ctaatgtttg gggttacttc tccgtgggta acaatggtaa tattcactcc 1500 ttttcggact ctcagttcgg ccatattttt gcttttggtg aaaatagaca agcttccagg 1560 aaacacatgg ttgttgccct gaaggaattg tccattaggg gtgatttcag aactactgtg 1620 gaatacttga tcaaactttt ggaaactgaa gatttcgagg ataacactat taccaccggt 1680 tggttggacg atttgattac tcataaaatg accgctgaaa agcctgatcc aactcttgcc 1740 gtcatttgcg gtgccgctac aaaggctttc ttagcatctg aagaagcccg ccacaagtat 1800 atcgaatcct tacaaaaggg acaagttcta tctaaagacc tactgcaaac tatgttccct 1860 gtagatttta tccatgaggg taaaagatac aagttcaccg tagctaaatc cggtaatgac 1920 cgttacacat tatttatcaa tggttctaaa tgtgatatca tactgcgtca actagctgat 1980 ggtggtcttt tgattgccat aggcggtaaa tcgcatacca tctattggaa agaagaagtt 2040 gctgctacaa gattatccgt tgactctatg actactttgt tggaagttga aaacgatcca 2100 acccagttgc gtactccatc ccctggtaaa ttggttaaat tcttggtgga aaatggtgaa 2160 cacattatca agggccaacc atatgcagaa attgaagtta tgaaaatgca aatgcctttg 2220 gtttctcaag aaaatggtat cgtccagtta ttaaagcaac ctggttctac cattgttgca 2280 ggtgatatca tggctattat gactcttgac gatccatcca aggtcaagca cgctctacca 2340 tttgaaggta tgctgccaga ttttggttct ccagttatcg aaggaaccaa acctgcctat 2400 aaattcaagt cattagtgtc tactttggaa aacattttga agggttatga caaccaagtt 2460 attatgaacg cttccttgca acaattgata gaggttttga gaaatccaaa actgccttac 2520 tcagaatgga aactacacat ctctgcttta cattcaagat tgcctgctaa gctagatgaa 2580 caaatggaag agttagttgc acgttctttg agacgtggtg ctgttttccc agctagacaa 2640 ttaagtaaat tgattgatat ggccgtgaag aatcctgaat acaaccccga caaattgctg 2700 ggcgccgtcg tggaaccatt ggcggatatt gctcataagt actctaacgg gttagaagcc 2760 catgaacatt ctatatttgt ccatttcttg gaagaatatt acgaagttga aaagttattc 2820 aatggtccaa atgttcgtga ggaaaatatc attctgaaat tgcgtgatga aaaccctaaa 2880 gatctagata aagttgcgct aactgttttg tctcattcga aagtttcagc gaagaataac 2940 ctgatcctag ctatcttgaa acattatcaa ccattgtgca agttatcttc taaagtttct 3000 gccattttct ctactcctct acaacatatt gttgaactag aatctaaggc taccgctaag 3060 gtcgctctac aagcaagaga aattttgatt caaggcgctt taccttcggt caaggaaaga 3120 actgaacaaa ttgaacatat cttaaaatcc tctgttgtga aggttgccta tggctcatcc 3180 aatccaaagc gctctgaacc agatttgaat atcttgaagg acttgatcga ttctaattac 3240 gttgtgttcg atgttttact tcaattccta acccatcaag acccagttgt gactgctgca 3300 gctgctcaag tctatattcg tcgtgcttat cgtgcttaca ccataggaga tattagagtt 3360 cacgaaggtg tcacagttcc aattgttgaa tggaaattcc aactaccttc agctgcgttc 3420 tccacctttc caactgttaa atctaaaatg ggtatgaaca gggctgttgc tgtttcagat 3480 ttgtcatatg ttgcaaacag tcagtcatct ccgttaagag aaggtatttt gatggctgtg 3540 gatcatttag atgatgttga tgaaattttg tcacaaagtt tggaagttat tcctcgtcac 3600 caatcttctt ctaacggacc tgctcctgat cgttctggta gctccgcatc gttgagtaat 3660 gttgctaatg tttgtgttgc ttctacagaa ggtttcgaat ctgaagagga aattttggta 3720 aggttgagag aaattttgga tttgaataag caggaattaa tcaatgcttc tatccgtcgt 3780 atcacattta tgttcggttt taaagatggg tcttatccaa agtattatac ttttaacggt 3840 ccaaattata acgaaaatga aacaattcgt cacattgagc cggctttggc cttccaactg 3900 gaattaggaa gattgtccaa cttcaacatt aaaccaattt tcactgataa tagaaacatc 3960 catgtctacg aagctgttag taagacttct ccattggata agagattctt tacaagaggt 4020 attattagaa cgggtcatat ccgtgatgac atttctattc aagaatatct gacttctgaa 4080 gctaacagat tgatgagtga tatattggat aatttagaag tcaccgacac ttcaaattct 4140 gatttgaatc atatcttcat caacttcatt gcggtgtttg atatctctcc agaagatgtc 4200 gaagccgcct tcggtggttt cttagaaaga tttggtaaga gattgttgag attgcgtgtt 4260 tcttctgccg aaattagaat catcatcaaa gatcctcaaa caggtgcccc agtaccattg 4320 cgtgccttga tcaataacgt ttctggttat gttatcaaaa cagaaatgta caccgaagtc 4380 aagaacgcaa aaggtgaatg ggtatttaag tctttgggta aacctggatc catgcattta 4440 agacctattg ctactcctta ccctgttaag gaatggttgc aaccaaaacg ttataaggca 4500 cacttgatgg gtaccacata tgtctatgac ttcccagaat tattccgcca agcatcgtca 4560 tcccaatgga aaaatttctc tgcagatgtt aagttaacag atgatttctt tatttccaac 4620 gagttgattg aagatgaaaa cggcgaatta actgaggtgg aaagagaacc tggtgccaac 4680 gctattggta tggttgcctt taagattact gtaaagactc ctgaatatcc aagaggccgt 4740 caatttgttg ttgttgctaa cgatatcaca ttcaagatcg gttcctttgg tccacaagaa 4800 gacgaattct tcaataaggt tactgaatat gctagaaagc gtggtatccc aagaatttac 4860 ttggctgcaa actcaggtgc cagaattggt atggctgaag agattgttcc actatttcaa 4920 gttgcatgga atgatgctgc caatccggac aagggcttcc aatacttata cttaacaagt 4980 gaaggtatgg aaactttaaa gaaatttgac aaagaaaatt ctgttctcac tgaacgtact 5040 gttataaacg gtgaagaaag atttgtcatc aagacaatta ttggttctga agatgggtta 5100 ggtgtcgaat gtctacgtgg atctggttta attgctggtg caacgtcaag ggcttaccac 5160 gatatcttca ctatcacctt agtcacttgt agatccgtcg gtatcggtgc ttatttggtt 5220 cgtttgggtc aaagagctat tcaggtcgaa ggccagccaa ttattttaac tggtgctcct 5280 gcaatcaaca aaatgctggg tagagaagtt tatacttcta acttacaatt gggtggtact 5340 caaatcatgt ataacaacgg tgtttcacat ttgactgctg ttgacgattt agctggtgta 5400 gagaagattg ttgaatggat gtcttatgtt ccagccaagc gtaatatgcc agttcctatc 5460 ttggaaacta aagacacatg ggatagacca gttgatttca ctccaactaa tgatgaaact 5520 tacgatgtaa gatggatgat tgaaggtcgt gagactgaaa gtggatttga atatggtttg 5580 tttgataaag ggtctttctt tgaaactttg tcaggatggg ccaaaggtgt tgtcgttggt 5640 agagcccgtc ttggtggtat tccactgggt gttattggtg ttgaaacaag aactgtcgag 5700 aacttgattc ctgctgatcc agctaatcca aatagtgctg aaacattaat tcaagaacct 5760 ggtcaagttt ggcatccaaa ctccgccttc aagactgctc aagctatcaa tgactttaac 5820 aacggtgaac aattgccaat gatgattttg gccaactgga gaggtttctc tggtggtcaa 5880 cgtgatatgt tcaacgaagt cttgaagtat ggttcgttta ttgttgacgc attggtggat 5940 tacaaacaac caattattat ctatatccca cctaccggtg aactaagagg tggttcatgg 6000 gttgttgtcg atccaactat caacgctgac caaatggaaa tgtatgccga cgtcaacgct 6060 agagctggtg ttttggaacc acaaggtatg gttggtatca agttccgtag agaaaaattg 6120 ctggacacca tgaacagatt ggatgacaag tacagagaat tgagatctca attatccaac 6180 aagagtttgg ctccagaagt acatcagcaa atatccaagc aattagctga tcgtgagaga 6240 gaactattgc caatttacgg acaaatcagt cttcaatttg ctgatttgca cgataggtct 6300 tcacgtatgg tggccaaggg tgttatttct aaggaactgg aatggaccga ggcacgtcgt 6360 ttcttcttct ggagattgag aagaagattg aacgaagaat atttgattaa aaggttgagc 6420 catcaggtag gcgaagcatc aagattagaa aagatcgcaa gaattagatc gtggtaccct 6480 gcttcagtgg accatgaaga tgataggcaa gtcgcaacat ggattgaaga aaactacaaa 6540 actttggacg ataaactaaa gggtttgaaa ttagagtcat tcgctcaaga cttagctaaa 6600 aagatcagaa gcgaccatga caatgctatt gatggattat ctgaagttat caagatgtta 6660 tctaccgatg ataaagaaaa attgttgaag actttgaaat aa 6702 <210> 30 <211> 6702 <212> DNA <213> ACC1sc_S1157A <400> 30 atgagcgaag aaagcttatt cgagtcttct ccacagaaga tggagtacga aattacaaac 60 tactcagaaa gacatacaga acttccaggt catttcattg gcctcaatac agtagataaa 120 ctagaggagt ccccgttaag ggactttgtt aagagtcacg gtggtcacac ggtcatatcc 180 aagatcctga tagcaaataa tggtattgcc gccgtgaaag aaattagatc cgtcagaaaa 240 tgggcatacg agacgttcgg cgatgacaga accgtccaat tcgtcgccat ggccacccca 300 gaagatctgg aggccaacgc agaatatatc cgtatggccg atcaatacat tgaagtgcca 360 ggtggtacta ataataacaa ctacgctaac gtagacttga tcgtagacat cgccgaaaga 420 gcagacgtag acgccgtatg ggctggctgg ggtcacgcct ccgagaatcc actattgcct 480 gaaaaattgt cccagtctaa gaggaaagtc atctttattg ggcctccagg taacgccatg 540 aggtctttag gtgataaaat ctcctctacc attgtcgctc aaagtgctaa agtcccatgt 600 attccatggt ctggtaccgg tgttgacacc gttcacgtgg acgagaaaac cggtctggtc 660 tctgtcgacg atgacatcta tcaaaagggt tgttgtacct ctcctgaaga tggtttacaa 720 aaggccaagc gtattggttt tcctgtcatg attaaggcat ccgaaggtgg tggtggtaaa 780 ggtatcagac aagttgaacg tgaagaagat ttcatcgctt tataccacca ggcagccaac 840 gaaattccag gctcccccat tttcatcatg aagttggccg gtagagcgcg tcacttggaa 900 gttcaactgc tagcagatca gtacggtaca aatatttcct tgttcggtag agactgttcc 960 gttcagagac gtcatcaaaa aattatcgaa gaagcaccag ttacaattgc caaggctgaa 1020 acatttcacg agatggaaaa ggctgccgtc agactgggga aactagtcgg ttatgtctct 1080 gccggtaccg tggagtatct atattctcat gatgatggaa aattctactt tttagaattg 1140 aacccaagat tacaagtcga gcatccaaca acggaaatgg tctccggtgt taacttacct 1200 gcagctcaat tacaaatcgc tatgggtatc cctatgcata gaataagtga cattagaact 1260 ttatatggta tgaatcctca ttctgcctca gaaatcgatt tcgaattcaa aactcaagat 1320 gccaccaaga aacaaagaag acctattcca aagggtcatt gtaccgcttg tcgtatcaca 1380 tcagaagatc caaacgatgg attcaagcca tcgggtggta ctttgcatga actaaacttc 1440 cgttcttcct ctaatgtttg gggttacttc tccgtgggta acaatggtaa tattcactcc 1500 ttttcggact ctcagttcgg ccatattttt gcttttggtg aaaatagaca agcttccagg 1560 aaacacatgg ttgttgccct gaaggaattg tccattaggg gtgatttcag aactactgtg 1620 gaatacttga tcaaactttt ggaaactgaa gatttcgagg ataacactat taccaccggt 1680 tggttggacg atttgattac tcataaaatg accgctgaaa agcctgatcc aactcttgcc 1740 gtcatttgcg gtgccgctac aaaggctttc ttagcatctg aagaagcccg ccacaagtat 1800 atcgaatcct tacaaaaggg acaagttcta tctaaagacc tactgcaaac tatgttccct 1860 gtagatttta tccatgaggg taaaagatac aagttcaccg tagctaaatc cggtaatgac 1920 cgttacacat tatttatcaa tggttctaaa tgtgatatca tactgcgtca actatctgat 1980 ggtggtcttt tgattgccat aggcggtaaa tcgcatacca tctattggaa agaagaagtt 2040 gctgctacaa gattatccgt tgactctatg actactttgt tggaagttga aaacgatcca 2100 acccagttgc gtactccatc ccctggtaaa ttggttaaat tcttggtgga aaatggtgaa 2160 cacattatca agggccaacc atatgcagaa attgaagtta tgaaaatgca aatgcctttg 2220 gtttctcaag aaaatggtat cgtccagtta ttaaagcaac ctggttctac cattgttgca 2280 ggtgatatca tggctattat gactcttgac gatccatcca aggtcaagca cgctctacca 2340 tttgaaggta tgctgccaga ttttggttct ccagttatcg aaggaaccaa acctgcctat 2400 aaattcaagt cattagtgtc tactttggaa aacattttga agggttatga caaccaagtt 2460 attatgaacg cttccttgca acaattgata gaggttttga gaaatccaaa actgccttac 2520 tcagaatgga aactacacat ctctgcttta cattcaagat tgcctgctaa gctagatgaa 2580 caaatggaag agttagttgc acgttctttg agacgtggtg ctgttttccc agctagacaa 2640 ttaagtaaat tgattgatat ggccgtgaag aatcctgaat acaaccccga caaattgctg 2700 ggcgccgtcg tggaaccatt ggcggatatt gctcataagt actctaacgg gttagaagcc 2760 catgaacatt ctatatttgt ccatttcttg gaagaatatt acgaagttga aaagttattc 2820 aatggtccaa atgttcgtga ggaaaatatc attctgaaat tgcgtgatga aaaccctaaa 2880 gatctagata aagttgcgct aactgttttg tctcattcga aagtttcagc gaagaataac 2940 ctgatcctag ctatcttgaa acattatcaa ccattgtgca agttatcttc taaagtttct 3000 gccattttct ctactcctct acaacatatt gttgaactag aatctaaggc taccgctaag 3060 gtcgctctac aagcaagaga aattttgatt caaggcgctt taccttcggt caaggaaaga 3120 actgaacaaa ttgaacatat cttaaaatcc tctgttgtga aggttgccta tggctcatcc 3180 aatccaaagc gctctgaacc agatttgaat atcttgaagg acttgatcga ttctaattac 3240 gttgtgttcg atgttttact tcaattccta acccatcaag acccagttgt gactgctgca 3300 gctgctcaag tctatattcg tcgtgcttat cgtgcttaca ccataggaga tattagagtt 3360 cacgaaggtg tcacagttcc aattgttgaa tggaaattcc aactaccttc agctgcgttc 3420 tccacctttc caactgttaa atctaaaatg ggtatgaaca gggctgttgc tgtttcagat 3480 ttgtcatatg ttgcaaacag tcagtcatct ccgttaagag aaggtatttt gatggctgtg 3540 gatcatttag atgatgttga tgaaattttg tcacaaagtt tggaagttat tcctcgtcac 3600 caatcttctt ctaacggacc tgctcctgat cgttctggta gctccgcatc gttgagtaat 3660 gttgctaatg tttgtgttgc ttctacagaa ggtttcgaat ctgaagagga aattttggta 3720 aggttgagag aaattttgga tttgaataag caggaattaa tcaatgcttc tatccgtcgt 3780 atcacattta tgttcggttt taaagatggg tcttatccaa agtattatac ttttaacggt 3840 ccaaattata acgaaaatga aacaattcgt cacattgagc cggctttggc cttccaactg 3900 gaattaggaa gattgtccaa cttcaacatt aaaccaattt tcactgataa tagaaacatc 3960 catgtctacg aagctgttag taagacttct ccattggata agagattctt tacaagaggt 4020 attattagaa cgggtcatat ccgtgatgac atttctattc aagaatatct gacttctgaa 4080 gctaacagat tgatgagtga tatattggat aatttagaag tcaccgacac ttcaaattct 4140 gatttgaatc atatcttcat caacttcatt gcggtgtttg atatctctcc agaagatgtc 4200 gaagccgcct tcggtggttt cttagaaaga tttggtaaga gattgttgag attgcgtgtt 4260 tcttctgccg aaattagaat catcatcaaa gatcctcaaa caggtgcccc agtaccattg 4320 cgtgccttga tcaataacgt ttctggttat gttatcaaaa cagaaatgta caccgaagtc 4380 aagaacgcaa aaggtgaatg ggtatttaag tctttgggta aacctggatc catgcattta 4440 agacctattg ctactcctta ccctgttaag gaatggttgc aaccaaaacg ttataaggca 4500 cacttgatgg gtaccacata tgtctatgac ttcccagaat tattccgcca agcatcgtca 4560 tcccaatgga aaaatttctc tgcagatgtt aagttaacag atgatttctt tatttccaac 4620 gagttgattg aagatgaaaa cggcgaatta actgaggtgg aaagagaacc tggtgccaac 4680 gctattggta tggttgcctt taagattact gtaaagactc ctgaatatcc aagaggccgt 4740 caatttgttg ttgttgctaa cgatatcaca ttcaagatcg gttcctttgg tccacaagaa 4800 gacgaattct tcaataaggt tactgaatat gctagaaagc gtggtatccc aagaatttac 4860 ttggctgcaa actcaggtgc cagaattggt atggctgaag agattgttcc actatttcaa 4920 gttgcatgga atgatgctgc caatccggac aagggcttcc aatacttata cttaacaagt 4980 gaaggtatgg aaactttaaa gaaatttgac aaagaaaatt ctgttctcac tgaacgtact 5040 gttataaacg gtgaagaaag atttgtcatc aagacaatta ttggttctga agatgggtta 5100 ggtgtcgaat gtctacgtgg atctggttta attgctggtg caacgtcaag ggcttaccac 5160 gatatcttca ctatcacctt agtcacttgt agatccgtcg gtatcggtgc ttatttggtt 5220 cgtttgggtc aaagagctat tcaggtcgaa ggccagccaa ttattttaac tggtgctcct 5280 gcaatcaaca aaatgctggg tagagaagtt tatacttcta acttacaatt gggtggtact 5340 caaatcatgt ataacaacgg tgtttcacat ttgactgctg ttgacgattt agctggtgta 5400 gagaagattg ttgaatggat gtcttatgtt ccagccaagc gtaatatgcc agttcctatc 5460 ttggaaacta aagacacatg ggatagacca gttgatttca ctccaactaa tgatgaaact 5520 tacgatgtaa gatggatgat tgaaggtcgt gagactgaaa gtggatttga atatggtttg 5580 tttgataaag ggtctttctt tgaaactttg tcaggatggg ccaaaggtgt tgtcgttggt 5640 agagcccgtc ttggtggtat tccactgggt gttattggtg ttgaaacaag aactgtcgag 5700 aacttgattc ctgctgatcc agctaatcca aatagtgctg aaacattaat tcaagaacct 5760 ggtcaagttt ggcatccaaa ctccgccttc aagactgctc aagctatcaa tgactttaac 5820 aacggtgaac aattgccaat gatgattttg gccaactgga gaggtttctc tggtggtcaa 5880 cgtgatatgt tcaacgaagt cttgaagtat ggttcgttta ttgttgacgc attggtggat 5940 tacaaacaac caattattat ctatatccca cctaccggtg aactaagagg tggttcatgg 6000 gttgttgtcg atccaactat caacgctgac caaatggaaa tgtatgccga cgtcaacgct 6060 agagctggtg ttttggaacc acaaggtatg gttggtatca agttccgtag agaaaaattg 6120 ctggacacca tgaacagatt ggatgacaag tacagagaat tgagatctca attatccaac 6180 aagagtttgg ctccagaagt acatcagcaa atatccaagc aattagctga tcgtgagaga 6240 gaactattgc caatttacgg acaaatcagt cttcaatttg ctgatttgca cgataggtct 6300 tcacgtatgg tggccaaggg tgttatttct aaggaactgg aatggaccga ggcacgtcgt 6360 ttcttcttct ggagattgag aagaagattg aacgaagaat atttgattaa aaggttgagc 6420 catcaggtag gcgaagcatc aagattagaa aagatcgcaa gaattagatc gtggtaccct 6480 gcttcagtgg accatgaaga tgataggcaa gtcgcaacat ggattgaaga aaactacaaa 6540 actttggacg ataaactaaa gggtttgaaa ttagagtcat tcgctcaaga cttagctaaa 6600 aagatcagaa gcgaccatga caatgctatt gatggattat ctgaagttat caagatgtta 6660 tctaccgatg ataaagaaaa attgttgaag actttgaaat aa 6702 <210> 31 <211> 6747 <212> DNA <213> ACC1ke <400> 31 atgagtgaag aaaatttatt tgaagattca tctgcaaaac gtcaatatga aataacagat 60 tactccaaga aacataaggc attggctcca cattttgtag ggttaaatac acttgacaaa 120 gtcgaagaat ctccactaaa agattttgtt aagtctcatg gtggtcatac tgttatctcc 180 aaaattttaa ttgctaataa tggtattgcc gcagttaaag aaattcgttc agttagaaaa 240 tggtcttatg aaacatttgg tgatgaaaga actgttcaat ttgttgcaat ggctactcca 300 gaagatttac aagctaattc tgaatatatt cgtatggctg atcaatacgt cgaagttcca 360 ggtggtacaa ataataacaa ttatgccaac gttgatctaa ttgttgatgt agcagaaaga 420 acagatgtcg atgcagtatg ggccggttgg ggtcatgctt ctgaaaatcc acatttacca 480 gaaaaattgg ctgcatcaaa gagaaagatt atattcattg gtcctccagg tagcgctatg 540 agatctttag gtgataaaat ttcttctaca attgttgctc aacatgccaa ggttccttgt 600 atcccatggt caggtactgg tattgataaa gttcacgttg acgaaacctc tggtttagtg 660 tctgttgatg atgatgttta ccaacaaggt tgttgccaat ctccagaaga tggtttagcc 720 aaagctaaac agattggttt cccagttatg atcaaagctt ccgaaggtgg tggtggtaaa 780 ggtatcagac aagttgaacg tgaagaagac tttattgcat tataccatca agctgctaat 840 gaaattccag gttctccaat ctttatcatg aagttagctg gcaaggctcg tcatttggaa 900 gttcaattat tggcagacca atatggtaca aatatttctc tatttggtcg tgattgttct 960 gttcaaagac gtcaccaaaa aattatcgaa gaagctccag ttacaattgc taacaatgaa 1020 acttttgaca agatggctaa cgctgccgtt agattaggta aattggttgg ttacgtatca 1080 gctggtacag tcgagtatct atattcaaat gaagagaaga aatactattt cttagaatta 1140 aacccaagat tacaagtgga gcatccaact acagaaatgg tctctggtgt taatttacca 1200 tctgcacaat tacaaattgc tatgggtata cctatgcaca gaattagcga tattagagtg 1260 ttatatggta tgaatccaca ttctgctact gaaatcgatt tcgaatttaa gactgaagaa 1320 gctaagaaga cacaaagaaa accagttcca aagggtcatt gtaccgcttg tcgtattaca 1380 tccgaagatc caaacgaagg attcaaacca tctggtggtt ctttacacga attgaatttc 1440 agatcctcat ctaatgtttg gggttatttc tctgtcggta acaacggtgg tatccattca 1500 ttctccgatt ctcagtttgg tcatatcttt gcctttggtg aaaacagaca aatgtcaaga 1560 aaacatatgg tggtagcatt aaaggaactc tctattagag gtgatttcag aactactgtt 1620 gaatatttga tcaaactttt ggaaaccgaa gattttgaag aaaatacaat tacaactggt 1680 tggttagatg atctaatttc tcataagatg actgcagaaa aacctgacgc tacgttagct 1740 gttatctgtg gtgctgctac aaaggctttt atcgcttctg aatctgctcg tatggaatac 1800 attgctcaat taaagagagg tcaagtccca gctaagaacc tattagagac tatgttccct 1860 gtcgaattca ttcatgaaaa taagagatac aaattcactg ttgctaaatc tgctgacgac 1920 tcttatactt tatttattaa tggttccaag tgtgaagtcc gtgccgttaa attatctgat 1980 ggtggtttgc taatatctgt tggtggtcaa tcccatacca tctattggaa ggaagaagct 2040 caagctacca gattatctat cgattctatg accactttac tagaagttga aaatgatcca 2100 acccaattac gtactccatc cccaggtaaa ttagttaaat tcttagttga aaatggcgat 2160 caaattaagg taggccaagc atatgccgaa attgaagtta tgaaaatgca aatgcctctg 2220 gtttctcaag aaaatggtgt aatacaatta ttgaaacaac ctggttctac tattgctgcc 2280 ggtgatatta ttgctatgct tgctctagat gatccatcca aggttaaaca tgctttacct 2340 tttgaaggta tgttaccaga attaggttcc ccaatggtcg aaggtactaa gcctgcttat 2400 aaattcaagt cattagtttc tactttagaa aatatattaa agggttacga taaccaagtc 2460 atcatgaatg catctctaca acaattaatt gaagttttaa gagatccaag attaccatac 2520 tctgaatgga aactatgtgt ttctgcatta cactccagat tgccaatgtc acttgatgaa 2580 aaactagatc aacttgttga ccgttctagt aatagaaatg ctatcttccc agctaaacaa 2640 ttaaacaaga tgattgaaga tgctgttaga gaacctggat ctgatgagtt actgccaaca 2700 atcgttgagc cactattaga tatcaccaat cgttatgcca atggtcttga agctcacgaa 2760 cattccgttt ttgtcaaatt tttggaagaa tactacaatg tcgaaaagtt gtttagttcc 2820 catggtaacc gtgaagaaaa tgtcattttg aaattacgtg atgaaaatct agattcttta 2880 gacaaggtcg tcgaaatagt cttatcgcat gctaaggtat ctgccaagaa cactttaatc 2940 ctagctattt tgaagcacta ccaaccaatt tgtaagatgt cttcttctgt tgctcatatt 3000 tttagtaaat cattaggtca catcgtggaa ttagaatcaa agtccgctgc caaggttgcg 3060 ttacaagcaa gagaaatctt aatccaaggt gctttaccat ctgttaaaga aagaaccgaa 3120 caaattgaac acatcctaag atcttctgtt acaaacacat cttatggtga tttggctcca 3180 aagaaatcag aaccaaactt agaaattcta aaggatttaa tcgattccaa ctatgttgtc 3240 tttgatgttc tgttaccatt tttatgccac gaagatccag tcatcgctac tgctgctgct 3300 caagtttacg tgcgtcgtgc ttacagagct tacactgttg gtgaagttaa gactccaaat 3360 attaaggatt catctattag tccattaatt gaatggaaat tccaattgcc ttctgctgca 3420 ttttcttcta ttccacaggt caagagcaaa attggtatga acagagctat gtctgtttct 3480 gatctttctt acgttgttga taatgaacat gctcctttaa gaacaggtat tttagttgcg 3540 tctgaacatt tagacgatgt cgatgcttgt ctagcccaag gtttggaagc tattccagaa 3600 catgctaata ctgctaatgg tccagctcca gatagatctg gtaattcatc ctctttgagt 3660 aatgttgcca atgtctgtgt ctcaagtact gaaggttttg aatctgaaaa agatatttta 3720 gcaagagttc acgaaatctt ggatttgaac aaatctgatt taatcaagtc ttctatccgt 3780 cgtattacat tcatgtttgg tttcgcagat ggtacatatc caaagtatta cacattcaac 3840 ggtccaaatt accaagaaaa tcaaactatt cgtcatatcg agccagcttg tgcattccaa 3900 ttggaattag gaagaatgtc taactttaat atcaagccaa tctttacaga aaatagaaat 3960 atccatgttt atgaagctat cagtaaaaca tctgcattag ataagagatt ctttaccaga 4020 ggtattatta gaactggtcg tatccgtgac gaaatttcaa ttcaagaata tcttacttct 4080 gaagctaaca agttaatgag tgacatttta gataatctag agattgttga cacatctaac 4140 tctgatttga accatatctt tatcaacttt tctgctgtct ttgatgtttc tccagaggat 4200 gttgaagctg cttttggtgg gttcttagaa agattcggga agagattctt aagacttcgt 4260 gtttcagctg ctgaaattcg tattattatt caagacccaa agacaggtgc ggctgttcca 4320 ttacgtgctt taatcaacaa tgtttctggt tacgtagtta aatccgagct atacactgaa 4380 attaagaatg ataagggaga atgggtcttt aagtcactag gtaaacctgg ttcaatgcat 4440 ttgagaccaa ttgctactcc atatccagtt aaggaatggt tacaacctaa acgttataag 4500 gctcatttaa tgggtaccac ttatctatac gatttcccag aattattcca tcaagctgcc 4560 attaccacat gggagaaata tctatctaac tcgtcagcta aggaattatc atctataaag 4620 gacagagacg atttatttat ttctcacgaa ttgatcgaag atgaaaatgg tgaattaact 4680 gaggtcgaaa gagaacctgg tgccaatact attggtatgg ttgcatttaa ggtcacaatg 4740 aagactccag aatatccaag aggtcgtcaa tttgtaattg ttgctaatga tatcactttc 4800 aagattggtt cctttggtcc acaagaagat gaatttttca acaaagttac tgaatatgct 4860 agaaagagag gtattccaag aatatacctg gctgctaact ctggtgccag aattggtatc 4920 gctgaagaat tggtgccatt attccaagtt gcttggaagg attctaaggt tccagcaaag 4980 ggtttcgaat atttatattt gagcaaagaa ggtatggaaa cattaaagag ttattctaag 5040 cagaattctg ttatcactga acgtgtcgtt gaagaaggtg aagaaagata tgttatcaaa 5100 tctattattg gtgctgatga tggtttaggt gttgaatgtt taagaggttc cggtttaatt 5160 gccggtgcta catcaagagc ttaccaagat attttcacaa ttactttagt tacatgtaga 5220 tctgtcggta ttggtgctta tttggttaga ttaggtcaaa gagccattca aattgaaggt 5280 caaccaatta ttttaactgg tgcaccagct atcaataaag tgttaggtag agaagtttat 5340 tcttctaatt tacaacttgg tggtactcaa attatgtaca ataatggtgt ttctcattta 5400 acagcttcag atgatttaca agctgttgaa caaatcatta attggttatc ttatgttccg 5460 gcaaagcgta atatgcctgt tccaatctta gaaaccgaag ataaatggga tagagccatt 5520 gatttcagac caactaccaa tgaacaatat gatgtcagat ggttaatcca aggtcgtgaa 5580 ttagacaacg gtgatttcga atacggtatg tttgataagg gttccttctt tgaaacttta 5640 tctggatggg ctaaaggtgt tgttgttggt agagctcgtt taggtggtat tccaatcggt 5700 gtcattggtg ttgaaacaag aaccattgaa aatgttattc cagctgatcc agcaaatcca 5760 gattctaccg aaactgttgt ccaagaagct ggtcaagttt ggtatccaaa ttctgcattt 5820 aagacggcac aagctattca agactttaac cacggtgaac aattaccatt aatgattcta 5880 gccaattgga gaggattctc tggtggtcaa cgtgatatgt acaatgaagt attgaaatat 5940 ggttcattta ttgttgatgc cttggttgat tacaaacaac ctatctttat ctatattcca 6000 ccaactggtg aattaagagg tggttcatgg gtcgtcgttg atccaactat taataatgat 6060 catatggaaa tgtatgctga taaagattcc cgtgctggtg tgttagaacc ttcaggtatg 6120 gttggtatta aataccgtag agagaaattg ttatctacta taacaagatt agattctacc 6180 tatagagaat tgaaagaaaa attatctgat aaattgttat ctactgaaga acatcaaaag 6240 atttctatac aactagctga acgtgaaaag atgttaattc caatctataa tcaaatcagt 6300 gtccaatttg ctgatctaca tgatagatca tctcgtatgt taaagaaagg tgttattaaa 6360 gctgaattga catgggaaga atctcgtcgt tatttcttct ggagattaag aagaagatta 6420 gatgaagaat acttattaag aagattagat tctcaattaa cccaagcttc aagattagag 6480 aagattgcac gtgttagatc ctggtatcca acttctgtcg atgtggagaa cgataaagaa 6540 gtagttacat ggattgaaga aaattatgaa acattagaag agaaattcaa agcccttaaa 6600 ttggaatcat ttgctttaga tttagctaaa aatattagaa atgatcatga aaatagtatg 6660 aagggtttag aagaagttgt tcgtatgtta agtgcaacag ataaagaaag attaaataag 6720 attattaatc cagatgataa aaaataa 6747 <210> 32 <211> 6651 <212> DNA <213> ACC1mc <400> 32 atggtcgaac ataagagctt gcctggccac tttttaggtg gtaattcact cgaatctgct 60 cctcagggtc ccgtcaaaga ctttgtccaa gcacatgagg gtcacacagt catttccaaa 120 gtcttgattg ccaacaacgg tatggctgca atgaaggaga tccgttccgt aagaaagtgg 180 gcttacgaga catttggtaa cgagagagct atcgaattca ctgtcatggc aacacccgag 240 gatttgaagg ccaatgctga atacatccgt atggccgata actttgtcga ggttcccggt 300 ggatccaaca acaacaacta tgccaatgtt gaactgattg tcgacgttgc cgaaagaact 360 gctgttcatg ctgtttgggc cggttggggt catgcttccg agaatcctcg tttgcctgaa 420 atgcttgcca agtccaagca caagtgtttg tttattggtc ctcctgcctc tgccatgaga 480 tcattaggtg acaaaatttc atctacaatc gttgcacaga gcgctcaagt ccccacaatg 540 ggttggagtg gtgatggtat taccgagaca gagtttgatg ctgcaggcca cgttatcgtc 600 cctgacaatg cttataacga agcctgtgtc aagactgccg agcaaggtct caaggctgcc 660 gagaagattg gtttccctgt catgatcaag gcctccgaag gtggtggagg taaaggtatt 720 cgtatggtca aggatggatc caactttgcc caattgtttg cccaagtgca aggtgaaatc 780 cctggttctc ccatctttat catgaagctg gctggtaacg ctcgccactt ggaagtccaa 840 ttgttggccg atcaatacgg taatgctatc agtttgtttg gtcgtgattg ttcagttcaa 900 cgtcgtcacc aaaagatcat cgaagaagct cctgtcacca ttgccaagcc tgatgtcttt 960 gagcaaatgg agaaggctgc tgtcagactc ggtaaattgg tgggctatgt ctctgctggt 1020 accgtggaat atctctacag tcatcacgac gatcaattct acttcttgga attgaaccct 1080 agattgcaag tcgagcatcc taccaccgag atggtttctg gcgtcaattt gcctgctgct 1140 cagctccaaa ttgctatggg tattcctctt caccgtatcc gcgatattcg tgtcctctac 1200 ggtgtccaac ccaacagtgc ctctgaaatc gatttcggtt tcgagcaccc cacctctctc 1260 accagccatc gtcgccccac ccccaagggc cacgttattg cttgtcgtat cactgctgag 1320 aatcccgatg ctggtttcaa gccttcttct ggtatcatgc aagaactcaa tttcagatcg 1380 agcacaaatg tttggggtta cttttctgtt gtctctgctg gtggtttgca cgagtatgct 1440 gattctcaat ttggtcacat ctttgcctat ggtgagaacc gtcaacaagc tcgcaagaac 1500 atggttattg ctctcaagga gctctccatc cgtgctgatt tccgtagtac cgttgaatac 1560 attatccgtt tgctcgagac ccctgatttc gaagagaaca ccatcaacac cggttggttg 1620 gatatgttga tttccaagaa gttgactgcc gaacgtcctg ataccatgtt ggctgtcttc 1680 tgtggtgctg tcaccaaggc tcatatggcc tctttggatt gtttccaaca atacaagcaa 1740 tccctcgaga agggtcaagt gcccagcaag ggttctctca agactgtctt tactgttgat 1800 ttcatctacg aggaagtgcg ctacaacttt actgtcaccc aatctgctcc tggtatctac 1860 accttgtatt tgaacggcac caagactcaa gttggtatcc gcgatttgtc tgatggcggt 1920 ctcttgatct ctattgacgg taaatctcac actacttact ctcgcgatga agttcaagcc 1980 acccgtatga tggtggatgg taagacctgt cttttggaaa aggagagtga tcctactcaa 2040 ttgagaagtc cttctcctgg taagcttgtc aacttgctcg ttgagaatgg cgatcatttg 2100 aatgctggtg atgcatatgc tgaaattgag gtcatgaaaa tgtacatgcc cttgattgct 2160 actgaagatg gtcatgttca attcatcaag caagctggtg ctactcttga ggctggtgat 2220 atcattggta tcttgtctct ggatgatcct agccgtgtca agcacgccct tcccttcaac 2280 ggcactgtac ctgcctttgg tgctcctcac atcactggtg acaagcctgt gcaacgcttc 2340 aacgctacca agctcacctt gcaacacatc ctccaaggtt acgataacca agctcttgtc 2400 caaactgttg tcaaggattt tgccgatatc ttgaacaacc ctgacttgcc ttactctgaa 2460 ttgaactctg ttctctctgc tttgtctggc agaattcctc aacgcttgga agcctctatc 2520 cacaagttgg ctgatgaatc caaggctgct aaccaagagt tccctgctgc tcaatttgaa 2580 aagcttgtcg aagactttgc tcgtgaacac atcacccttc aatctgaagc cactgcttac 2640 aagaactctg tcgctccctt gagcagcatc tttgctcgct atagaaacgg tctcaccgaa 2700 cacgcctaca gcaactatgt tgaattgatg gaagcttact acgacgtcga aatcttgttc 2760 aaccaacaac gcgaagagga ggtcatcttg tcactcagag atcagcacaa ggacgacttg 2820 gacaaggtgt tggctgttac tttgtctcat gccaaggtca acatcaagaa caatgtgatc 2880 ctcatgttgc tcgacctcat caaccctgtc tccactggca gtgctcttga caagtacttt 2940 actcctatcc ttaagcgttt gtctgaaatc gaaagccgtg ccactcaaaa ggtcactctc 3000 aaggcccgtg aattgttaat cctctgtcaa ttgccttctt acgaggaacg tcaagctcaa 3060 atgtaccaaa ttctcaagaa ctctgtcacc gagtctgtct acggcggtgg ttctgaatac 3120 cgcactccca gctacgacgc cttcaaggat ctcattgaca ccaagttcaa cgtctttgat 3180 gtcttgcctc acttcttcta ccatgctgat ccttatattg ccttggctgc tatcgaagta 3240 tactgtcgtc gttcttacca cgcttacaag attttggatg tcgcttacaa cctcgagcac 3300 aagccttacg ttgttgcctg gaagttcttg ctccaaactg ctgctaacgg cattgactcc 3360 aacaagcgta ttgcctctta ctctgatctt accttcttgc tcaacaagac tgaggaagag 3420 cctatccgta ctggtgccat gactgcatgc aactctttgg ctgatctcca agccgagctt 3480 cctcgcatct tgactgcctt tgaggaagag cctctccctc ccatgttgca acgcaatgct 3540 gctcccaagg aagaacgcat ggagaacatt ctcaacattg ctgttcgtgc tgatgaggac 3600 atggatgaca ctgctttcag aaccaagatc tgtgagatga tcactgccaa tgctgatgct 3660 ttccgtcaag cccatttgcg tcgtctttcc gttgtcgtct gccgtgataa ccaatggccc 3720 gattactaca ctttccgtga gcgtgaaaac taccaagaag acgagaccat ccgtcacatt 3780 gaacctgcca tggcctatca actcgagttg gctcgtctct ccaactttga catcaagccc 3840 tgtttcattg agaacagaca aatgcacgtc tactatgctg ttgccaagga gaacccctct 3900 gactgccgtt tcttcatccg tgccttggtc cgtcccggtc gtgtcaagag ctccatgcgc 3960 actgctgact acttgatctc tgagagtgat cgtctcttga ctgacattct tgacaccctc 4020 gaaattgtca gccacgagta caagaactct gactgtaacc acttgttcat caacttcatc 4080 cccacttttg ctattgaggc tgatgatgtg gagcacgcct tgaaggactt tgtcgatcgt 4140 catggtaagc gtctctggaa gctccgtgtc actggtgccg aaatccgctt caacgtgcaa 4200 tccaagaagc ctgatgcccc catcatcccc atgcgtttca ccgtggacaa cgtctctggc 4260 ttcatcttga aggtcgaggt ctaccaagaa gtcaagactg aaaagagtgg atggattctc 4320 aagtctgtca acaagatccc tggtgccatg cacatgcagc ccttgtctac gccttatcct 4380 accaaggaat ggctccaacc ccgtcgttac aaggctcact tgatgggcac cacctacgtc 4440 tatgacttcc ctgagctctt ccgtcaatct gttcaaaacc aatggactca agccatcaag 4500 cgcaaccctc tcttgaagca acccagccat ttggttgaag ccaaggagtt ggtcctcgac 4560 gaagatgatg tcttgcaaga gattgaccgt gctcccggta ccaacactgt cggtatggtt 4620 gcctggatca tgactattcg cactcccgag tatcccagtg gtcgtcgcat cattgctatt 4680 gccaacgaca ttaccttcaa gattggttcc tttggtgttg ctgaagatca agtcttttac 4740 aaggcatctg agctcgctcg tgctcttggt attcctcgta tctacttgtc tgccaactcg 4800 ggtgctcgta tcggtcttgc tgaagagctc atcagccaat tcagagctgc ctggaaggat 4860 gcttccaacc ctactgctgg tttcaactac ctctacttga ctcctgccga gtatgatact 4920 cttgttcaac aaggtgatgc caagagtgtg ctcgtggaag agatccaaga tgaaggtgaa 4980 acgcgtctcc gtatcactga tgtcattggc cacactgatg gtcttggtgt ggagaacttg 5040 aagggttctg gtctcattgc tggtgccacc tcgcgtgcct atgatgatat cttcaccatc 5100 acgcttgtca cttgtcgttc cgtcggtatt ggtgcttacc ttgtccgtct cggtcaaaga 5160 actgtccaaa acgaaggcca acccatcatc ttgaccggtg ctcctgcctt gaacaaggtg 5220 cttggtcgtg aagtctacac ctccaacttg cagttgggtg gtactcaaat catgtacaag 5280 aacggtgtct ctcacttgac tgccgagaat gatttggaag gtattgccaa gattgtgcaa 5340 tggctctcat tcgtgcctga tgtccgcaac gctcctgtct ctatgcgctt gggtgttgat 5400 cccattgacc gtgacattga gtacactcct cccaagggtc cctctgatcc tcgcttcttc 5460 ttggccggta agagtgagaa cggcaagtgg ttgagcggtt tctttgatca agactccttc 5520 attgagacct tgagtggttg ggctcgtacc gttgtcgttg gtcgtgctcg tttgggtggt 5580 attcctatgg gtgttgtctc tgttgagacc cgtactgtgg agaacattgt tcctgctgat 5640 cccgccaatt ctgattccac tgagcaagtc tttatggaag ctggtggtgt ctggttccct 5700 aactctgctt acaagactgc ccaagccatc aatgacttca acaagggtga gcaattgcct 5760 ttgatgatct ttgccaactg gcgtggtttc tctggtggtc aacgcgacat gtacaacgaa 5820 gtcctcaagt acggtgctca aatcgtggat gctctcagca actacaagca acctgtcttt 5880 gtctacatta ttcccaacgg tgagcttcgt ggtggtgctt gggtcgttgt tgatcctacc 5940 atcaacaagg acatgatgga aatgtacgct gataacaatg ctcgtggtgg tgtcttggag 6000 cccgagggta tcgtcgagat caagtaccgt aagcctgcct tgttggctac tatggagcgt 6060 cttgacgcta cttatgcttc tttgaagaag caattggccg aagaaggtaa atctgacgaa 6120 gagaaggctg ccctcaaggt gcaaatcgaa gctcgtgaac aagaactctt gcccgtatac 6180 caacaaatct ctatccaatt tgctgatctc catgatcgtg ctggtcgtat gaaggctaag 6240 ggtgtcatcc gcaaggctct tgactggcgt cgtgctcgtc actacttcta ctggcgtgtc 6300 cgtcgtcgtc tctgtgaaga gtacactttc cgcaagattg ttgctgccac tagcgctgct 6360 cccatgtctc gcgagcaaat gcttgatctc gtcaagcaat ggtttaccag cgacaatgaa 6420 actgccagct tcgaagatgc tgatgaactc gtctctgaat ggtttgaaaa gcgtgccagt 6480 gtcattgatc aacgtatcag caagttgaag agcgatgcta ccaaggaaca agtcgtgtct 6540 ctcggtaatg ccgatcaaga agctgtcatt gaaggtttca ctcaattgat tgaaaacttg 6600 agcgaagatc aacgtgctga aatcttgcgc aagcttaatt ctcgtttcta a 6651 <210> 33 <211> 6702 <212> DNA <213> ACC1sc <400> 33 atgagcgaag aaagcttatt cgagtcttct ccacagaaga tggagtacga aattacaaac 60 tactcagaaa gacatacaga acttccaggt catttcattg gcctcaatac agtagataaa 120 ctagaggagt ccccgttaag ggactttgtt aagagtcacg gtggtcacac ggtcatatcc 180 aagatcctga tagcaaataa tggtattgcc gccgtgaaag aaattagatc cgtcagaaaa 240 tgggcatacg agacgttcgg cgatgacaga accgtccaat tcgtcgccat ggccacccca 300 gaagatctgg aggccaacgc agaatatatc cgtatggccg atcaatacat tgaagtgcca 360 ggtggtacta ataataacaa ctacgctaac gtagacttga tcgtagacat cgccgaaaga 420 gcagacgtag acgccgtatg ggctggctgg ggtcacgcct ccgagaatcc actattgcct 480 gaaaaattgt cccagtctaa gaggaaagtc atctttattg ggcctccagg taacgccatg 540 aggtctttag gtgataaaat ctcctctacc attgtcgctc aaagtgctaa agtcccatgt 600 attccatggt ctggtaccgg tgttgacacc gttcacgtgg acgagaaaac cggtctggtc 660 tctgtcgacg atgacatcta tcaaaagggt tgttgtacct ctcctgaaga tggtttacaa 720 aaggccaagc gtattggttt tcctgtcatg attaaggcat ccgaaggtgg tggtggtaaa 780 ggtatcagac aagttgaacg tgaagaagat ttcatcgctt tataccacca ggcagccaac 840 gaaattccag gctcccccat tttcatcatg aagttggccg gtagagcgcg tcacttggaa 900 gttcaactgc tagcagatca gtacggtaca aatatttcct tgttcggtag agactgttcc 960 gttcagagac gtcatcaaaa aattatcgaa gaagcaccag ttacaattgc caaggctgaa 1020 acatttcacg agatggaaaa ggctgccgtc agactgggga aactagtcgg ttatgtctct 1080 gccggtaccg tggagtatct atattctcat gatgatggaa aattctactt tttagaattg 1140 aacccaagat tacaagtcga gcatccaaca acggaaatgg tctccggtgt taacttacct 1200 gcagctcaat tacaaatcgc tatgggtatc cctatgcata gaataagtga cattagaact 1260 ttatatggta tgaatcctca ttctgcctca gaaatcgatt tcgaattcaa aactcaagat 1320 gccaccaaga aacaaagaag acctattcca aagggtcatt gtaccgcttg tcgtatcaca 1380 tcagaagatc caaacgatgg attcaagcca tcgggtggta ctttgcatga actaaacttc 1440 cgttcttcct ctaatgtttg gggttacttc tccgtgggta acaatggtaa tattcactcc 1500 ttttcggact ctcagttcgg ccatattttt gcttttggtg aaaatagaca agcttccagg 1560 aaacacatgg ttgttgccct gaaggaattg tccattaggg gtgatttcag aactactgtg 1620 gaatacttga tcaaactttt ggaaactgaa gatttcgagg ataacactat taccaccggt 1680 tggttggacg atttgattac tcataaaatg accgctgaaa agcctgatcc aactcttgcc 1740 gtcatttgcg gtgccgctac aaaggctttc ttagcatctg aagaagcccg ccacaagtat 1800 atcgaatcct tacaaaaggg acaagttcta tctaaagacc tactgcaaac tatgttccct 1860 gtagatttta tccatgaggg taaaagatac aagttcaccg tagctaaatc cggtaatgac 1920 cgttacacat tatttatcaa tggttctaaa tgtgatatca tactgcgtca actatctgat 1980 ggtggtcttt tgattgccat aggcggtaaa tcgcatacca tctattggaa agaagaagtt 2040 gctgctacaa gattatccgt tgactctatg actactttgt tggaagttga aaacgatcca 2100 acccagttgc gtactccatc ccctggtaaa ttggttaaat tcttggtgga aaatggtgaa 2160 cacattatca agggccaacc atatgcagaa attgaagtta tgaaaatgca aatgcctttg 2220 gtttctcaag aaaatggtat cgtccagtta ttaaagcaac ctggttctac cattgttgca 2280 ggtgatatca tggctattat gactcttgac gatccatcca aggtcaagca cgctctacca 2340 tttgaaggta tgctgccaga ttttggttct ccagttatcg aaggaaccaa acctgcctat 2400 aaattcaagt cattagtgtc tactttggaa aacattttga agggttatga caaccaagtt 2460 attatgaacg cttccttgca acaattgata gaggttttga gaaatccaaa actgccttac 2520 tcagaatgga aactacacat ctctgcttta cattcaagat tgcctgctaa gctagatgaa 2580 caaatggaag agttagttgc acgttctttg agacgtggtg ctgttttccc agctagacaa 2640 ttaagtaaat tgattgatat ggccgtgaag aatcctgaat acaaccccga caaattgctg 2700 ggcgccgtcg tggaaccatt ggcggatatt gctcataagt actctaacgg gttagaagcc 2760 catgaacatt ctatatttgt ccatttcttg gaagaatatt acgaagttga aaagttattc 2820 aatggtccaa atgttcgtga ggaaaatatc attctgaaat tgcgtgatga aaaccctaaa 2880 gatctagata aagttgcgct aactgttttg tctcattcga aagtttcagc gaagaataac 2940 ctgatcctag ctatcttgaa acattatcaa ccattgtgca agttatcttc taaagtttct 3000 gccattttct ctactcctct acaacatatt gttgaactag aatctaaggc taccgctaag 3060 gtcgctctac aagcaagaga aattttgatt caaggcgctt taccttcggt caaggaaaga 3120 actgaacaaa ttgaacatat cttaaaatcc tctgttgtga aggttgccta tggctcatcc 3180 aatccaaagc gctctgaacc agatttgaat atcttgaagg acttgatcga ttctaattac 3240 gttgtgttcg atgttttact tcaattccta acccatcaag acccagttgt gactgctgca 3300 gctgctcaag tctatattcg tcgtgcttat cgtgcttaca ccataggaga tattagagtt 3360 cacgaaggtg tcacagttcc aattgttgaa tggaaattcc aactaccttc agctgcgttc 3420 tccacctttc caactgttaa atctaaaatg ggtatgaaca gggctgtttc tgtttcagat 3480 ttgtcatatg ttgcaaacag tcagtcatct ccgttaagag aaggtatttt gatggctgtg 3540 gatcatttag atgatgttga tgaaattttg tcacaaagtt tggaagttat tcctcgtcac 3600 caatcttctt ctaacggacc tgctcctgat cgttctggta gctccgcatc gttgagtaat 3660 gttgctaatg tttgtgttgc ttctacagaa ggtttcgaat ctgaagagga aattttggta 3720 aggttgagag aaattttgga tttgaataag caggaattaa tcaatgcttc tatccgtcgt 3780 atcacattta tgttcggttt taaagatggg tcttatccaa agtattatac ttttaacggt 3840 ccaaattata acgaaaatga aacaattcgt cacattgagc cggctttggc cttccaactg 3900 gaattaggaa gattgtccaa cttcaacatt aaaccaattt tcactgataa tagaaacatc 3960 catgtctacg aagctgttag taagacttct ccattggata agagattctt tacaagaggt 4020 attattagaa cgggtcatat ccgtgatgac atttctattc aagaatatct gacttctgaa 4080 gctaacagat tgatgagtga tatattggat aatttagaag tcaccgacac ttcaaattct 4140 gatttgaatc atatcttcat caacttcatt gcggtgtttg atatctctcc agaagatgtc 4200 gaagccgcct tcggtggttt cttagaaaga tttggtaaga gattgttgag attgcgtgtt 4260 tcttctgccg aaattagaat catcatcaaa gatcctcaaa caggtgcccc agtaccattg 4320 cgtgccttga tcaataacgt ttctggttat gttatcaaaa cagaaatgta caccgaagtc 4380 aagaacgcaa aaggtgaatg ggtatttaag tctttgggta aacctggatc catgcattta 4440 agacctattg ctactcctta ccctgttaag gaatggttgc aaccaaaacg ttataaggca 4500 cacttgatgg gtaccacata tgtctatgac ttcccagaat tattccgcca agcatcgtca 4560 tcccaatgga aaaatttctc tgcagatgtt aagttaacag atgatttctt tatttccaac 4620 gagttgattg aagatgaaaa cggcgaatta actgaggtgg aaagagaacc tggtgccaac 4680 gctattggta tggttgcctt taagattact gtaaagactc ctgaatatcc aagaggccgt 4740 caatttgttg ttgttgctaa cgatatcaca ttcaagatcg gttcctttgg tccacaagaa 4800 gacgaattct tcaataaggt tactgaatat gctagaaagc gtggtatccc aagaatttac 4860 ttggctgcaa actcaggtgc cagaattggt atggctgaag agattgttcc actatttcaa 4920 gttgcatgga atgatgctgc caatccggac aagggcttcc aatacttata cttaacaagt 4980 gaaggtatgg aaactttaaa gaaatttgac aaagaaaatt ctgttctcac tgaacgtact 5040 gttataaacg gtgaagaaag atttgtcatc aagacaatta ttggttctga agatgggtta 5100 ggtgtcgaat gtctacgtgg atctggttta attgctggtg caacgtcaag ggcttaccac 5160 gatatcttca ctatcacctt agtcacttgt agatccgtcg gtatcggtgc ttatttggtt 5220 cgtttgggtc aaagagctat tcaggtcgaa ggccagccaa ttattttaac tggtgctcct 5280 gcaatcaaca aaatgctggg tagagaagtt tatacttcta acttacaatt gggtggtact 5340 caaatcatgt ataacaacgg tgtttcacat ttgactgctg ttgacgattt agctggtgta 5400 gagaagattg ttgaatggat gtcttatgtt ccagccaagc gtaatatgcc agttcctatc 5460 ttggaaacta aagacacatg ggatagacca gttgatttca ctccaactaa tgatgaaact 5520 tacgatgtaa gatggatgat tgaaggtcgt gagactgaaa gtggatttga atatggtttg 5580 tttgataaag ggtctttctt tgaaactttg tcaggatggg ccaaaggtgt tgtcgttggt 5640 agagcccgtc ttggtggtat tccactgggt gttattggtg ttgaaacaag aactgtcgag 5700 aacttgattc ctgctgatcc agctaatcca aatagtgctg aaacattaat tcaagaacct 5760 ggtcaagttt ggcatccaaa ctccgccttc aagactgctc aagctatcaa tgactttaac 5820 aacggtgaac aattgccaat gatgattttg gccaactgga gaggtttctc tggtggtcaa 5880 cgtgatatgt tcaacgaagt cttgaagtat ggttcgttta ttgttgacgc attggtggat 5940 tacaaacaac caattattat ctatatccca cctaccggtg aactaagagg tggttcatgg 6000 gttgttgtcg atccaactat caacgctgac caaatggaaa tgtatgccga cgtcaacgct 6060 agagctggtg ttttggaacc acaaggtatg gttggtatca agttccgtag agaaaaattg 6120 ctggacacca tgaacagatt ggatgacaag tacagagaat tgagatctca attatccaac 6180 aagagtttgg ctccagaagt acatcagcaa atatccaagc aattagctga tcgtgagaga 6240 gaactattgc caatttacgg acaaatcagt cttcaatttg ctgatttgca cgataggtct 6300 tcacgtatgg tggccaaggg tgttatttct aaggaactgg aatggaccga ggcacgtcgt 6360 ttcttcttct ggagattgag aagaagattg aacgaagaat atttgattaa aaggttgagc 6420 catcaggtag gcgaagcatc aagattagaa aagatcgcaa gaattagatc gtggtaccct 6480 gcttcagtgg accatgaaga tgataggcaa gtcgcaacat ggattgaaga aaactacaaa 6540 actttggacg ataaactaaa gggtttgaaa ttagagtcat tcgctcaaga cttagctaaa 6600 aagatcagaa gcgaccatga caatgctatt gatggattat ctgaagttat caagatgtta 6660 tctaccgatg ataaagaaaa attgttgaag actttgaaat aa 6702 <210> 34 <211> 6801 <212> DNA <213> ACCyl <400> 34 atgcgactgc aattgaggac actaacacgt cggtttttca gtatggcttc aggatcttca 60 acgccagatg tggctccctt ggtggacccc aacattcaca aaggtctcgc ctctcatttc 120 tttggactca attctgtcca cacagccaag ccctcaaaag tcaaggagtt tgtggcttct 180 cacggaggtc atacagttat caacaaggtc ctcatcgcta acaacggtat tgccgcagta 240 aaggagatcc gttcagtacg aaaatgggcc tacgagacct ttggcgacga gcgagcaatc 300 tcgttcaccg tcatggccac ccccgaagat ctcgctgcca acgccgacta cattagaatg 360 gccgatcagt acgtcgaggt gcccggagga accaacaaca acaactacgc caacgtcgag 420 ctgattgtcg acgtggctga gcgattcggc gtcgatgccg tgtgggccgg atggggccat 480 gccagtgaaa atcccctgct ccccgagtcg ctagcggcct ctccccgcaa gattgtcttc 540 atcggccctc ccggagctgc catgagatct ctgggagaca aaatttcttc taccattgtg 600 gcccagcacg caaaggtccc gtgtatcccg tggtctggaa ccggagtgga cgaggttgtg 660 gttgacaaga gcaccaacct cgtgtccgtg tccgaggagg tgtacaccaa gggctgcacc 720 accggtccca agcagggtct ggagaaggct aagcagattg gattccccgt gatgatcaag 780 gcttccgagg gaggaggagg aaagggtatt cgaaaggttg agcgagagga ggacttcgag 840 gctgcttacc accaggtcga gggagagatc cccggctcgc ccatcttcat tatgcagctt 900 gcaggcaatg cccggcattt ggaggtgcag cttctggctg atcagtacgg caacaatatt 960 tcactgtttg gtcgagattg ttcggttcag cgacggcatc aaaagattat tgaggaggct 1020 cctgtgactg tggctggcca gcagaccttc actgccatgg agaaggctgc cgtgcgactc 1080 ggtaagcttg tcggatatgt ctctgcaggt accgttgaat atctgtattc ccatgaggac 1140 gacaagttct acttcttgga gctgaatcct cgtcttcagg tcgaacatcc taccaccgag 1200 atggtcaccg gtgtcaacct gcccgctgcc cagcttcaga tcgccatggg tatccccctc 1260 gatcgaatca aggacattcg tctcttttac ggtgttaacc ctcacaccac cactccaatt 1320 gatttcgact tctcgggcga ggatgctgat aagacacagc gacgtcccgt cccccgaggt 1380 cacaccactg cttgccgaat cacatccgag gaccctggag agggtttcaa gccctccgga 1440 ggtactatgc acgagctcaa cttccgatcc tcgtccaacg tgtggggtta cttctccgtt 1500 ggtaaccagg gaggtatcca ttcgttctcg gattcgcagt ttggtcacat cttcgccttc 1560 ggtgagaacc gaagtgcgtc tcgaaagcac atggttgttg ctttgaagga actatctatt 1620 cgaggtgact tccgaaccac cgtcgagtac ctcatcaagc tgctggagac accggacttc 1680 gaggacaaca ccatcaccac cggctggctg gatgagctta tctccaacaa gctgactgcc 1740 gagcgacccg actcgttcct cgctgttgtt tgtggtgctg ctaccaaggc ccatcgagct 1800 tccgaggact ctattgccac ttacatggct tcgctagaga agggccaggt ccctgctcga 1860 gacattctca agaccctttt ccccgttgac ttcatctacg agggccagcg gtacaagttc 1920 accgccaccc ggtcgtctga ggactcttac acgctgttca tcaacggttc tcgatgcgac 1980 attggagtta gacctctttc tgacggtggt attctgtgtc ttgtaggtgg gagatcccac 2040 aatgtctact ggaaggagga ggttggagcc acgcgactgt ctgttgactc caagacctgc 2100 cttctcgagg tggagaacga tcccactcag cttcgatctc cctctcccgg taagctggtt 2160 aagttcctgg tcgagaacgg cgaccacgtg cgagccaacc agccctatgc cgagattgag 2220 gtcatgaaga tgtacatgac tctcactgct caggaggacg gtattgtcca gctgatgaag 2280 cagcccggtt ccaccatcga ggctggcgac atcctcggta tcttggccct tgatgatcct 2340 tccaaggtca agcatgccaa gccctttgag ggccagcttc ccgagcttgg accccccact 2400 ctcagcggta acaagcctca tcagcgatac gagcactgcc agaacgtgct ccataacatt 2460 ctgcttggtt tcgataacca ggtggtgatg aagtccactc ttcaggagat ggttggtctg 2520 ctccgaaacc ctgagcttcc ttatctccag tgggctcatc aggtgtcttc tctgcacacc 2580 cgaatgagcg ccaagctgga tgctactctt gctggtctca ttgacaaggc caagcagcga 2640 ggtggcgagt ttcctgccaa gcagcttctg cgagcccttg agaaggaggc gagctctggc 2700 gaggtcgatg cgctcttcca gcaaactctt gctcctctgt ttgaccttgc tcgagagtac 2760 caggacggtc ttgctatcca cgagcttcag gttgctgcag gccttctgca ggcctactac 2820 gactctgagg cccggttctg cggacccaac gtacgtgacg aggatgtcat tctcaagctt 2880 cgagaggaga accgagattc tcttcgaaag gttgtgatgg cccagctgtc tcattctcga 2940 gtcggagcca agaacaacct tgtgctggcc cttctcgatg aatacaaggt ggccgaccag 3000 gctggcactg actctcctgc ctccaacgtg cacgttgcaa agtacttgcg acctgtgctg 3060 cgaaagattg tggagctgga atctcgagct tctgccaagg tatctctgaa agcccgagag 3120 atcctcatcc agtgcgctct gccctctcta aaggagcgaa ctgaccagct tgagcacatt 3180 ctgcgatctt ctgtcgtcga gtctcgatac ggagaggttg gtctggagca ccgaactccc 3240 cgagccgata ttctcaagga ggttgtcgac tccaagtaca ttgtctttga tgtgcttgcc 3300 cagttctttg cccacgatga tccctggatc gtccttgctg ccctggagct gtacatccga 3360 cgagcttgca aggcctactc catcctggac atcaactacc accaggactc ggacctgcct 3420 cccgtcatct cgtggcgatt tagactgcct accatgtcgt ctgctttgta caactcagta 3480 gtgtcttctg gctccaaaac ccccacttcc ccctcggtgt ctcgagctga ttccgtctcc 3540 gacttttcgt acaccgttga gcgagactct gctcccgctc gaaccggagc gattgttgcc 3600 gtgcctcatc tggatgatct ggaggatgct ctgactcgtg ttctggagaa cctgcccaaa 3660 cggggcgctg gtcttgccat ctctgttggt gctagcaaca agagtgccgc tgcttctgct 3720 cgtgacgctg ctgctgctgc cgcttcatcc gttgacactg gcctgtccaa catttgcaac 3780 gttatgattg gtcgggttga tgagtctgat gacgacgaca ctctgattgc ccgaatctcc 3840 caggtcattg aggactttaa ggaggacttt gaggcctgtt ctctgcgacg aatcaccttc 3900 tccttcggca actcccgagg tacttatccc aagtatttca cgttccgagg ccccgcatac 3960 gaggaggacc ccactatccg acacattgag cctgctctgg ccttccagct ggagctcgcc 4020 cgtctgtcca acttcgacat caagcctgtc cacaccgaca accgaaacat ccacgtgtac 4080 gaggctactg gcaagaacgc tgcttccgac aagcggttct tcacccgagg tatcgtacga 4140 cctggtcgtc ttcgagagaa catccccacc tcggagtatc tcatttccga ggctgaccgg 4200 ctcatgagcg atattttgga cgctctagag gtgattggaa ccaccaactc ggatctcaac 4260 cacattttca tcaacttctc agccgtcttt gctctgaagc ccgaggaggt tgaagctgcc 4320 tttggcggtt tcctggagcg atttggccga cgtctgtggc gacttcgagt caccggtgcc 4380 gagatccgaa tgatggtatc cgaccccgaa actggctctg ctttccctct gcgagcaatg 4440 atcaacaacg tctctggtta cgttgtgcag tctgagctgt acgctgaggc caagaacgac 4500 aagggccagt ggattttcaa gtctctgggc aagcccggct ccatgcacat gcggtctatc 4560 aacactccct accccaccaa ggagtggctg cagcccaagc ggtacaaggc ccatctgatg 4620 ggtaccacct actgctatga cttccccgag ctgttccgac agtccattga gtcggactgg 4680 aagaagtatg acggcaaggc tcccgacgat ctcatgactt gcaacgagct gattctcgat 4740 gaggactctg gcgagctgca ggaggtgaac cgagagcccg gcgccaacaa cgtcggtatg 4800 gttgcgtgga agtttgaggc caagaccccc gagtaccctc gaggccgatc tttcatcgtg 4860 gtggccaacg atatcacctt ccagattggt tcgtttggcc ctgctgagga ccagttcttc 4920 ttcaaggtga cggagctggc tcgaaagctc ggtattcctc gaatctatct gtctgccaac 4980 tctggtgctc gaatcggcat tgctgacgag ctcgttggca agtacaaggt tgcgtggaac 5040 gacgagactg acccctccaa gggcttcaag tacctttact tcacccctga gtctcttgcc 5100 accctcaagc ccgacactgt tgtcaccact gagattgagg aggagggtcc caacggcgtg 5160 gagaagcgtc atgtgatcga ctacattgtc ggagagaagg acggtctcgg agtcgagtgt 5220 ctgcggggct ctggtctcat tgcaggcgcc acttctcgag cctacaagga tatcttcact 5280 ctcactcttg tcacctgtcg atccgttggt atcggtgctt accttgttcg tcttggtcaa 5340 cgagccatcc agattgaggg ccagcccatc attctcactg gtgcccccgc catcaacaag 5400 ctgcttggtc gagaggtcta ctcttccaac ttgcagcttg gtggtactca gatcatgtac 5460 aacaacggtg tgtctcatct gactgcccga gatgatctca acggtgtcca caagatcatg 5520 cagtggctgt catacatccc tgcttctcga ggtcttccag tgcctgttct ccctcacaag 5580 accgatgtgt gggatcgaga cgtgacgttc cagcctgtcc gaggcgagca gtacgatgtt 5640 agatggctca tttctggccg aactctcgag gatggtgctt tcgagtctgg tctctttgac 5700 aaggactctt tccaggagac tctgtctggc tgggccaagg gtgttgttgt tggtcgagct 5760 cgtcttggcg gcattccctt cggtgtcatt ggtgtcgaga ctgcgaccgt cgacaatact 5820 acccctgccg atcccgccaa cccggactct attgagatga gcacctctga agccggccag 5880 gtttggtacc ccaactcggc cttcaagacc tctcaggcca tcaacgactt caaccatggt 5940 gaggcgcttc ctctcatgat tcttgctaac tggcgaggct tttctggtgg tcagcgagac 6000 atgtacaatg aggttctcaa gtacggatct ttcattgttg atgctctggt tgactacaag 6060 cagcccatca tggtgtacat ccctcccacc ggtgagctgc gaggtggttc ttgggttgtg 6120 gttgacccca ccatcaactc ggacatgatg gagatgtacg ctgacgtcga gtctcgaggt 6180 ggtgtgctgg agcccgaggg aatggtcggt atcaagtacc gacgagacaa gctactggac 6240 accatggctc gtctggatcc cgagtactcc tctctcaaga agcagcttga ggagtctccc 6300 gattctgagg agctcaaggt caagctcagc gtgcgagaga agtctctcat gcccatctac 6360 cagcagatct ccgtgcagtt tgccgacttg catgaccgag ctggccgaat ggaggccaag 6420 ggtgtcattc gtgaggctct tgtgtggaag gatgctcgtc gattcttctt ctggcgaatc 6480 cgacgacgat tagtcgagga gtacctcatt accaagatca atagcattct gccctcttgc 6540 actcggcttg agtgtctggc tcgaatcaag tcgtggaagc ctgccactct tgatcagggc 6600 tctgaccggg gtgttgccga gtggtttgac gagaactctg atgccgtctc tgctcgactc 6660 agcgagctca agaaggacgc ttctgcccag tcgtttgctt ctcaactgag aaaggaccga 6720 cagggtactc tccagggcat gaagcaggct ctcgcttctc tttctgaggc tgagcgggct 6780 gagctgctca aggggttgtg a 6801 <210> 35 <211> 6732 <212> DNA <213> ACC1ch <400> 35 atgagcgaag aaaacctgtt cgaggacgcc gccgcaaagc gccagtacga gataacagac 60 tactccacca agcacaagac cctcgcgtcc cacttcgtcg gcctcaacac cctcgagcgc 120 gtcgaggagt cccccctcaa ggacttcgtc aagtcccacg gcggtcacac cgtcatctcc 180 aagatcctca tcgccaacaa cggtatcgcc gccgtcaagg agatccgctc cgtcagaaag 240 tgggcctacg agaccttcgg cgatgagcgc accgtccagt tcgtcgccat ggccaccccc 300 gaggacctcc aggccaactc cgagtacatc cgcatggcgg accagtacgt cgaggtcccc 360 ggcggcacca acaacaacaa ctacgccaac gtcgacctca tcgtcgacgt cgccgagaga 420 accgacgtcg acgcagtctg ggccggctgg ggccacgcct cagagaaccc acacctccca 480 gagaagctcg ccgcctcaaa gagaaagatc atcttcatcg gtccacccgg cagcgccatg 540 cgctccctcg gtgacaagat ctcctccacc atcgtcgctc agcatgccaa ggtcccatgt 600 ataccttggt ccggcaccgg cgtcgacaca gtccacctcg acagcgagac cggcctagtg 660 tccgtcgacg acgacatcta ccagaagggc tgctgctcct cccccgagga cggcctcgcc 720 aaggccaaga agatcggctt cccagtaatg atcaaggcct ccgagggtgg tggtggtaag 780 ggtatcagac aggtcgagcg cgaggaggac ttcatcgccc tgtaccacca ggccgccaac 840 gagatcccgg gctccccaat attcatcatg aagctcgccg gcaaggcccg ccacctggag 900 gtccagcttc tcgccgatca gtacggtacc aacatttccc tgtttggccg tgactgttcc 960 gtgcagagac gccaccagaa gatcatcgag gaggcgccag tgactatcgc cagcaacgag 1020 acattcgaga agatggccag cgcagccgtc agactcggta agctggtcgg ctacgtctcc 1080 gccggtaccg tcgaatacct atattccagc gaggagaaga agtactactt cctggagctt 1140 aatccaagac tgcaggtcga gcacccaacc accgagatgg tctccggtgt caacctgcct 1200 tccgcccagc tgcagatcgc catgggtatc ccaatgcaca gaatcagcga catcagagtc 1260 ctttacggta tgaaccctca cggcgccacc gagatcgact tcgagttcaa gactgaggag 1320 gccaagaaga ctcagagaaa gcctgttcca aagggccatt gtaccgcttg tcgtatcact 1380 tccgaggatc caaacgaggg tttcaagcct tccggtggtt ccctacacga gctgaacttc 1440 agatcttctt caaatgtctg gggttacttc tccgtcggta acaacggtgg tatccactcc 1500 ttctccgact cccaattcgg tcacatcttc gccttcggtg agaacagaca gatgtcgaga 1560 aagcacatgg ttgtcgccct aaaggagctg tccatcagag gtgacttcag aactactgtc 1620 gagtatctga tcaagcttct agagactgag gatttcgagg agaacactat tactaccggt 1680 tggttggacg acctgatctc gcacaagatg accgcccaga agccagatgc tacgcttgcc 1740 gtcatttgtg gcgctgctac caaggctttc atcgcgtcag agtctgccag aaaggaatac 1800 attaaccagc tcaagagagg tcaggttcca ccaaagggcc tactggagac tatgttccca 1860 attgaattca tccacgagaa caagagatat aagttcactg ttgccaagtc tgctgacgat 1920 tcctacacac ttttcatcaa tggttccaag tgcgaggtcc gtgctgttaa gctatctgat 1980 ggcggtcttt tgatttctgt cggcggccaa tctcacacta tctactggaa ggaggaagcc 2040 caggctacca gactttcgat cgactctatg actactctac ttgaggttga gaatgatcct 2100 acacagctgc gcaccccatc cccaggtaag cttgtgaagt tccttgttga gaacggtgac 2160 caaatcaagg tcggccagcc atacgctgag attgaggtca tgaagatgca aatgccattg 2220 gtttctcagg agaacggtgt tatccagtta ctaaagcagc caggttctac cattgacgct 2280 ggtgatatca ttgccatgct tgcgctagac gacccatcca aggtcaagca cgctatgcca 2340 tttgagggta tgcttccaga gctaggctct ccaatggttg aaggtaccaa gccagcctac 2400 aagttccact ccctagtgtc cacgcttgag aatattctaa agggttacga caaccaggtt 2460 atcatgaacg catctctaca acagctaatc gaggttctca gagacccaag actaccatac 2520 tctgagtgga agttaaatgt atctgcgcta cacaccagac ttcctcttgc tttggacgag 2580 aagttggaca agctggtcga gcgttctgct aaccgcagtg ctatcttccc tgctaagcag 2640 ctacagaaga tgatcgatga tgctgtgaga gacccacaag ctgacccact attgtcatcc 2700 attgttgagc cactccttga cattactact cgttatgcca atggcctaga ggcacatgaa 2760 cattccgtct ttgtcaagtt cttagaggag tactacaacg tcgagaagct attcagcgag 2820 cacggtgagc gtgctgagaa tgtcatccta aagctacgtg acgagaacat cgatgaccta 2880 gacaaggttg ttctcattgt tctctctcat gccaaggttt ctgccaagaa cactctgatc 2940 ctagctattc tgaagcacta ccaaccaatt tgcaaaatgt cttcgtctgt tgcccatatc 3000 ttcaccaagc cacttcagca cattgtcgag ctggagtcca agcctgctgc taaggttgct 3060 ctacaggcca gagaaattct gatccagggt gccctaccat ctgtcaagga gagaacagag 3120 cagattgaac acattctaag atcttctgtc acaaacactt cttatggtga ctcttcgcca 3180 aaggtcagtg agccaaacat ggaaatactg aaggatctaa ttgactccaa ctatgtcgtt 3240 tttgatgttc tactcccatt cctgtgtcac gaagaccctg ttattgctac tgccgctggt 3300 caggtttatg ttcgccgtgc ttacagagct tacaccgttg gtgaggttaa gtcacacagc 3360 attactgact cttccatgaa cccattgatt gagtggaagt tccagctacc ctctgctgct 3420 ttctcttcca ttccacagct gaagagcaag attggtatga acagagctat gtctgtttct 3480 gacttgtcct acgttgttga cagtgagcac gctccactga gaactggtat attggttgct 3540 gctgaacatc tagatgaggt tgacgccagc cttgcacagg gcctagaggt tatcccagag 3600 cactccgctc caggcaatgg tccagctcca gacagatctg gcagttcctc ctctttgagt 3660 aacatcgtta atgtctgtgt tgccagcaca gaaggattcg attccgaaaa ggagattctt 3720 gccagagtga acgagatcct agagctgaac aaggctgatc taatcaagtc ttccatccgt 3780 cgtatcactt tcatgttcgg tttcccagac ggtacttatc caaagtatta cactttcaat 3840 ggtccaaact accaggagaa ccagacgatt cgtcacattg agccagcttg tgcgttccaa 3900 ctagaattag gaagaatgtc taacttcaac atcactccaa tcttcactga caacagaaac 3960 atccacgttt acgaagctgt cagtaagact tctgcactag acaagagatt cttcactaga 4020 ggtataatca gaaccggtcg cattcgtgac gagatttcca ttcaagaata tctgacctct 4080 gaagctaaca agctgatgag tgacattttg gacaacctgg agatcatcga cacttcgaac 4140 tccgacttga accacatctt tatcaacttt tctgctgtct ttgatgtgtc tccagaggat 4200 gtcgaggctg cctttggtgg cttcctagaa agattcggta agagattcct aagactacgt 4260 gtctctgctg ctgagattcg catcattatc caggacccaa agactggtgc accagtgcca 4320 ttgcgtgccc tgatcaacaa cgtctctggt tatgtcgtga agtctgagct atacaccgag 4380 atcaagaatg ccaagggtga gtgggtcttt aagtctctcg gtaagccagg ttctatgcat 4440 ctgagaccaa ttgctactcc atacccagtt aaggagtggc tacagccaaa gcgttacaag 4500 gctcacctaa tgggcacaac ttacgtttac gatttcccag agctcttcca ccaggctgct 4560 atcactacat ggcagaagta caagaccaac tcttctcaaa aggatctaac cacaatgaag 4620 gagagagatg atctgtttat ttcccacgag ctgattgagg atgagagcgg tgaactcact 4680 gaggttgaga gagaacctgg tgctaattca attggtatgg ttgccttcaa ggtcaccatg 4740 aagactcctg agtacccaag aggccgtcaa tttgtcattg tcgctaacga tatcacctat 4800 aagattggtt cctttggtcc acaagaggac gaattcttca acaaggttac cgagtacgcc 4860 agaaagagag gtattccaag aatctatcta gctgctaact ctggtgctag aatcggtatt 4920 gctgaggagc ttgttccact ataccaggtt gcctggaagg acgctgatgt tccagcgaag 4980 ggtttcgagt acatctacct gaacaaggat gaaatggaga ctctaaaggg tcactccaag 5040 gagaactctg tcatcaccga gcgcatcgtc gaggacggtg aggagagatt tgttatcaag 5100 gctattattg gtgctgacga cggcctaggt gttgagtgtt tgagaggttc tggtttgatt 5160 gctggtgcaa cttcgagagc ctaccaggac attttcacta ttactttggt tacctgcaga 5220 tccgttggta ttggtgctta tctagtcaga cttggccaga gagctattca gattgagggt 5280 cagcctatca tcctaactgg tgctcctgcc atcaacaagg ttctaggtag agaggtctac 5340 tcctccaacc tccaactcgg tggtacacag atcatgtaca acaacggtgt ttcccacttg 5400 actgcctctg acgatcttca ggcggttgag cagatcatta agtggttgtc ctacgtccct 5460 gcaaagcgca acatgccagt tccaatccta gagaacggcg ataagtggga cagacagatt 5520 gactacagac caactgttaa tgagcagtat gatgttcgct ggatgattga gggtcgcgaa 5580 ctagaagacg gtaggtttga atatggtatg ttcgacaagg gttctttctt tgagactctg 5640 tcgggctggg ctaagggtgt cgttgtcggc cgtgcccgtc taggcggtat tccaatcggt 5700 gttattggtg tggagaccag gaccatcgag aacgttatcc cagcggaccc tgccaaccca 5760 gactctactg aggctgttgt tcaggaagcc ggtcaagtgt ggtaccccaa ctctgctttc 5820 aagactgctc aggccatcaa ggactttaac catggtgagc agctgccgct aatgattcta 5880 gccaactgga gaggtttctc tggtggtcag cgcgacatgt acaacgaggt tctgaagtac 5940 ggttctttca ttgtcgactc cttagtggac tacaagcagc caatcttcat ctacattcca 6000 ccaaccggtg agttgcgtgg tggttcttgg gtcgttgttg accctactat caacgccgac 6060 cagatggaga tgtacgccga taaggactcc cgtgctggtg ttctagagcc aagtggtatg 6120 gttggtatta agtaccgtcg cgagaagcta ctggctacga tgaccagact cgacaccact 6180 taccgcgaac taaaggagca gctatccgac aagacgctaa cccctgctca acaccaggag 6240 gtatctgtga agcttgctga gcgtgagaag ttcctactac caatttacac tcaggtaagt 6300 gttcagtttg ctgatctgca tgaccgctct tctcgtatgg tgaagaaggg tgttatccgt 6360 gctgagctga cgtgggagga gtcccgtcgc ttcttcttct ggagactgag acgcaagctt 6420 gacgaggaat acctcgttaa gcgtctggac attgagttgc ctcacgcatc gcgtctggaa 6480 aagctggcgc gtctgcgttc atggtaccca gcctctatcg atgagaccaa ggaccgtgat 6540 gttgtgacat ggattgaaga gaactacaat gtgtttgagg aggagttcaa ggccttgaag 6600 cttgagtctt ttgcacagga gcttgccaag agcatgcgta ctgatcacga tgttacgatg 6660 cgcggtctcg aggagattgc ccgcatgttg tctaaggagg acaaggagag gctgctgagt 6720 gcgctaaagt aa 6732 <210> 36 <211> 3690 <212> DNA <213> HPDH-MCRbs <400> 36 atgaccgctg gtaaaggtaa aggtgctaaa gttgaccctg ctggtggtaa agccgctact 60 ggtagattgg ccggtaaagt tgcattggtc acaggtgcag ctggtaattt gggtggtgcc 120 attattagaa gattcattgc cgaaggtgca actttggtta tgactggtag aactaaggaa 180 agattagctg cagcaaagca agctttattg gacgaaactg gtgttgaaga agcaagagtt 240 tttaccgtaa tcttggatgg tgctgatcca gattcagtta gagcaggtat tgatcaagtc 300 agagctgcct taggtagaat tgatgttttg gtcaataatg caggttcagc tggtcctaga 360 caaacattgg aaaacgtacc attgactaga gccgaaatgg aagcaaatgg tgactcagaa 420 acagtagctg acgccatgag aaatttgttg ggtgtcagtt ggaacttaac aagagcagct 480 gccccaataa tgccagtagg tgcatcagtc gttaatattt ccaccatctt ctctcacaca 540 agatactatg gtagaacagc ctatgtcgtt cctaaggctg cattaaacgc cttatcccaa 600 gaattagcaa aggaattagg tgcatctggt ataagagtaa atacagtttt cccaggtcca 660 atcgaaagtg aaagaattag aacagttttc gcagctatgg atgccttgca aggtgaacca 720 gaaaactcta ctgcaaatca tttcatgggt agaatgacat tggaaagagc tatcggtgac 780 gcccctaaaa gaaagacttt gccagttcca ggtgacattg cagatgcttg tttgttttta 840 gcatcagatg aaagtgctgc cttaaatggt aacgaaatag atgttacaca tggtatgcaa 900 gttagaaaag atagtagatc aacttacatg actagaccat ccatgagatc tttggacggt 960 gcaggtttga ccgtcttaat tgccgctggt gaagcatggg aagatgcatt agatatggct 1020 aagatccaag ctgcatgtgg tgccagagtc ttattgggta tgactagaca agttgacgtc 1080 gcacaagccg aagccagagt taaagctcaa ggtgctgaag aaggtttaac catcatgaga 1140 ttcaacagag ctgaacctga tggtatggaa gcagccataa gagcctattc tgatgcacat 1200 ggtgcaatcc actctgccat cgttttacct gtaaagccag ctggtcattt cggtgactct 1260 ttggttgaag cttcagatgc agaagttgac gctttgattg atatcgaatt aaccggtgct 1320 attgcagttg ccagaacctt gagaagatac tggaaaacat gctctgactt gatgcaagat 1380 ccaagattcg tctttatgtc aggtccatct gatggtaaag gtaacaaagc aggtagaata 1440 ttagcagccg caatagccca attaatcaca atttggagag atgaatctag agtagacttt 1500 gcacaaggta gacaaaagag agccgcatgg ggtaatcaaa tagttagata cacaaatgaa 1560 gaagatgaaa acaccagatt cgccgcaggt catgctacta gaattgtttt caaggaacaa 1620 aagataactg aaacttcctt gtacttgcct gccaacattg gtgaagaaac tggtgctaga 1680 agagctatgt taggttttgc cgaaaatatc actggtttgc atttgggtaa agtcgccatg 1740 attacaggtg gttctgcagg tattggtggt caagttgcca gattattggc attggctggt 1800 gccaaagtta tgatggtagc tagaagagca agtgaattgg aagccgcaag agaaagaata 1860 gttggtgaat tgcaagatgt tggttttagt ggtgttgaaa gaagagttaa aatcatggca 1920 gatgttgacg tatccgactt tgattcttta agaagagctg ccgatgcaac tatggcagaa 1980 tttggtagaa tagactactt gattaataac gctggtgttg caggtgctga agatatggtt 2040 attgacatgt cattggatat gtggagatgg acattggacg ccaacttgat ttcaaactat 2100 ttgttaatgc actatgttgt cccaattatg aagagacaag gttccggtta tatcttgaat 2160 gtttcttcct acttcggtgg tgaaaagttt ttggcagttg cctatccaaa cagagctgat 2220 tacgcagtct ctaaagccgg tcaaagagca atggttgaat ccttctcaag atttttgggt 2280 ccagaaatcc aagttaatgc aattgctcct ggtcctgttg atggtgacag attgtcaggt 2340 actggtggta aaccaggttt attcttgaga agagccagat taatcttgga aaacaaaaga 2400 ttgaatgcaa tctatgctgc agttatcgaa tcaataagag aaggtgcaga agttacacac 2460 gttttgggta gattagccag aaacgatacc ggttacttgt cacacgacag atccacccct 2520 gacccattga gattattatg tttggaatta gccagagaag gtgacggtat ttgcacatgg 2580 gacagatact tattgacacc tgatattgca ggtagattgt taatgagatt ggcctcagca 2640 ggttacttat tggcacatga agaatggcaa caaagaagtg catcagcaga aggtagaact 2700 ggttggttat tggcattacc acctgacgat atgccatatt tgcctcaaga aagaatcggt 2760 aaagaagcag ataaagtcgg tagaggtgtt ttgagtcaat tacatttggg tgcaatgcca 2820 aaggaatcag aagttgccca agcaacagta ttttacttgg ctgatagagc agtttccggt 2880 gaaacattca tgccttctgg tggtttgtcc gtagaaagat cctacaccga aagagaaatg 2940 tttggttccc ctaaacaaga aagattggaa caaatgaagg gtaaaacagt atgggtcatc 3000 ggtgaacact tagccgatta tatcgccgaa actgcaaatc acttaatcgg tggtttaggt 3060 gttgcaagat tggtcttatt gacaagatct gaagccggtg gtgcttcagt tttggctttg 3120 ttagaccctg aaatcgcaga aagagttacc gtcagattgt gtggtgcaga tgtagaatca 3180 gctatggatc aagctttaac agacttcggt agacctacca cagtagttag tactcctatg 3240 gcaggtttac cagacagatt gtttgaagat ggtgcaatgt tagaaccaga agagtttaga 3300 actttgtgcg aagaaaattt gacacaacac tttagagttt ccagaaaggc atccttatat 3360 gatggttgcc aattggtttt agtatcacca gatgtcccat ttggtcaatc cggtcctgcc 3420 tttgcattag ccaacttcgt caagacaact ttgcatgctt tcaccgcaac tttagctgta 3480 gaaaacgaaa gattagttca tgatgttcct accaatcaaa tcaacttgac cagaagagta 3540 agaagtgaag aaccaagaaa cgctgtagaa caccaagaag aattgaagag atttgctaga 3600 gcagttttgt tggttggtgc acctttgcca gatgcccaag attccagata cagagcaaga 3660 atctacagag gtacaagtat gacagtttaa 3690 <210> 37 <211> 3663 <212> DNA <213> HPDH-MCRca <400> 37 atgagtggta caggtagatt ggctggtaaa atagcattga taacaggtgg tgcaggtaat 60 ataggttccg aattgacaag aagatttttg gctgaaggtg caactgttat catatcaggt 120 agaaatagag ctaaattaac tgctttggcc gaaagaatgc aagcagaagc aggtgttcca 180 gctaaaagaa tcgacttaga agtcatggac ggttccgatc ctgtagctgt tagagctggt 240 atagaagcta ttgttgcaag acacggtcaa atcgacatct tggtaaataa tgctggttct 300 gcaggtgctc aaagaagatt ggctgaaatc cctttgactg aagcagaatt gggtccaggt 360 gctgaagaaa ccttgcatgc ttctattgca aacttattgg gtatgggttg gcatttgatg 420 agaatagctg cacctcatat gcctgtaggt tctgccgtaa ttaacgtttc caccatattc 480 tcaagagcag aatactatgg tagaatccca tatgttaccc caaaagcagc cttgaatgca 540 ttgtcacaat tggctgccag agaattgggt gccagaggta tcagagttaa taccatcttt 600 ccaggtccta ttgaatccga tagaatcaga accgtatttc aaagaatgga ccaattgaag 660 ggtagacctg aaggtgacac agcacatcat ttcttgaata ccatgagatt gtgtagagcc 720 aatgaccaag gtgcattaga aagaagattt ccatcagtag gtgacgttgc agatgcagct 780 gtatttttag cttcagctga atctgccgca ttgtctggtg aaaccatcga agtaacacat 840 ggtatggaat tgccagcttg ttctgaaaca tctttgttgg caagaacaga tttgagaact 900 atagatgcat ctggtagaac aaccttaata tgtgctggtg accaaattga agaagttatg 960 gctttgactg gtatgttaag aacttgtggt tctgaagtta ttataggttt cagatccgct 1020 gcagctttgg cacaattcga acaagccgtt aatgaatcta gaagattggc aggtgctgat 1080 tttacccctc caatcgcttt accattggac ccaagagatc ctgctacaat tgatgcagtt 1140 tttgattggg gtgcaggtga aaatactggt ggtatccacg cagctgttat cttgcctgca 1200 acctctcatg aaccagcccc ttgtgttata gaagttgatg acgaaagagt cttgaacttc 1260 ttggctgatg aaatcacagg tactatcgta atcgcttcta gattggcaag atactggcaa 1320 tctcaaagat tgacaccagg tgccagagca agaggtccaa gagtaatttt cttgtcaaac 1380 ggtgctgatc aaaatggtaa tgtatatggt agaatacaat cagctgcaat aggtcaattg 1440 atcagagttt ggagacacga agctgaattg gattatcaaa gagcttctgc tgcaggtgac 1500 catgtattgc ctccagtttg ggctaatcaa atcgtaagat tcgcaaatag atctttagaa 1560 ggtttggaat ttgcctgtgc ttggaccgct caattgttac attctcaaag acacatcaat 1620 gaaatcacct tgaatatccc tgctaacatc tcagcaacca caggtgcaag atccgcttca 1680 gttggttggg ccgaatcttt aataggtttg cacttgggta aagttgcatt gatcacaggt 1740 ggttctgctg gtatcggtgg tcaaatcggt agattattgg cattgtcagg tgcaagagta 1800 atgttagcag caagagacag acacaagtta gaacaaatgc aagccatgat acaaagtgag 1860 ttagccgaag taggttacac tgacgtagaa gatagagtcc atatagcacc aggttgtgat 1920 gtctcctccg aagcccaatt agccgatttg gttgaaagaa ctttgtccgc tttcggtaca 1980 gttgattatt tgattaataa cgccggtatc gctggtgtag aagaaatggt tatagatatg 2040 ccagtcgaag gttggagaca tacattattc gcaaatttga tctccaacta cagtttgatg 2100 agaaagttgg ctcctttgat gaaaaagcaa ggttctggtt acatcttgaa tgtttcttca 2160 tactttggtg gtgaaaaaga tgctgcaatt ccatatccta acagagcaga ctacgccgta 2220 tcaaaggcag gtcaaagagc tatggctgaa gtttttgcta gattcttagg tccagaaatc 2280 caaattaatg caattgcccc aggtcctgtt gaaggtgaca gattgagagg tactggtgaa 2340 agacctggtt tgttcgctag aagagcaaga ttaatcttgg aaaataagag attaaacgaa 2400 ttgcatgccg ctttgatagc agccgctaga acagatgaaa gatctatgca cgaattagta 2460 gaattgttgt tgccaaatga cgttgcagcc ttggaacaaa acccagctgc acctactgct 2520 ttaagagaat tggcaagaag attcagatct gaaggtgacc cagctgcttc ttcttcttct 2580 gcattgttga atagatcaat tgcagccaag ttattggcta gattacataa cggtggttat 2640 gtattgccag ccgatatatt tgctaatttg ccaaacccac cagatccatt tttcacaaga 2700 gcacaaattg atagagaagc cagaaaagtt agagatggta taatgggcat gttgtacttg 2760 caaagaatgc caactgaatt tgatgttgct atggcaacag tctattactt agctgacaga 2820 aatgtttctg gtgaaacttt tcatccatca ggtggtttga gatatgaaag aactcctaca 2880 ggtggtgaat tattcggttt gccatcccct gaaagattag ctgaattggt cggtagtacc 2940 gtatacttaa ttggtgaaca tttgactgaa cacttgaatt tgttggccag agcttatttg 3000 gaaagatacg gtgcaagaca agttgtcatg attgttgaaa ccgaaactgg tgctgaaacc 3060 atgagaagat tattgcatga tcacgtcgaa gccggtagat tgatgactat tgtagctggt 3120 gaccaaatag aagctgcaat cgaccaagct attacaagat atggtagacc aggtcctgta 3180 gtttgtacac cttttagacc attacctacc gtccctttgg taggcagaaa agattctgac 3240 tggtcaaccg ttttatctga agccgaattt gctgaattat gcgaacatca attgactcat 3300 cacttcagag ttgcaagaaa gattgccttg tccgatggtg caagtttagc cttggtcacc 3360 ccagaaacta cagcaacttc caccactgaa caattcgcat tggccaactt catcaagaca 3420 accttgcacg ctttcacagc aaccatcggt gtcgaaagtg aaagaactgc tcaaagaatc 3480 ttaattaacc aagtagattt gacaagaaga gctagagcag aagaaccaag agatccacat 3540 gaaagacaac aagaattaga aagattcatt gaagctgttt tattggtcac agcaccattg 3600 ccacctgaag ccgataccag atacgctggt agaatacata gaggtagagc tataacagtt 3660 taa 3663 <210> 38 <211> 3660 <212> DNA <213> HPDH-MCRcag <400> 38 atgtccgtca caggtagatt ggcaggtaaa atagccttga taacaggtgg tgctggtaac 60 ataggttcag aaatgactag aagattctta gccgaaggtg caactgttat tatctcaggt 120 agaaactcag ctaaattggc cgctttagca gaaagattga gatccgaagc aggtgttcct 180 gctaagagaa tagatttgga agttatggac ggttcagatc cagctgctgt tagagcaggt 240 gttgcagcta tcattggtag acatggtcac atagatatct tagttaataa tgcaggttcc 300 accggtgcac aaagaagatt ggctgaaatc ccattaaatg aaaccgatag agacttagac 360 gatgaagaag ctttgtctac aagtgttgca aatttgttgg gtatggcttg gcacttaatg 420 agaatcttaa gtcctcatat gccaccaggt tctgctatta taaacatctc caccatcttc 480 agtagagctg aatactatgg tagaatccct tacgtcgttc caaaggcagc cttgaataca 540 ttgactcaaa tcgctgcaag agaattgggt atcagaggta tcagagttaa cacaatcttt 600 ccaggtccaa ttgaatccga aagaatccaa acagtttttc aaagaatgga ccaattgaaa 660 ggtagaccag aaggtgacac agcatcacaa ttcttggcaa ctatgagatt atacagagct 720 aacgatcaag gtcaattgga aagaagattc ccaacaatct gtgacgttgc tgatgctgca 780 gttttcttgg cctctgatga agcagccgct ttgacaggtg aaactatcga agttacacat 840 ggtatggaat tgccaacatc ttcagaaacc tctttattgg ctagaacaga cttaagaact 900 attgacgcaa atggtagaac cactttgata tgtgccggtg accaaatcga agaagttatg 960 gctttgactg gtatgttgag aacttgtggt tctgaagtca ttatcggttt tagaagtgaa 1020 gctgcattgg cacaattcga acaagccatc ggtgaatcta gaagattggc tggtgaatcc 1080 tttatcccac ctattgcatt accaattgac ttgagaaacc caagtacaat agacgcatta 1140 tttgattggg ctggtgaaaa tactggtggt attcatgcag ccgtaatctt gccagcatca 1200 ggtagagaac ctgcaacaca agttatagac atcgacgatg ctcatgtcca agccttctta 1260 aacgatgaaa tcgtaggttc catcattatc gcatcaagat tggctagata ctggcaagca 1320 caaagaatcg cacctggtgc cagagcaaga gaacctagag tcatcttctt gtctaatggt 1380 gcatcaactg ctggtaatcc ttacggtaga atccaatccg cagctataga acaattaata 1440 agagtttgga gacatgaagc agccttggat tacgaaagag caaccgctgc aggtgaaaga 1500 gttttgcctg cagtttgggc atcacaaatc gttagatttg caaacagatc cttagaaggt 1560 ttggaatttg cctgtgcatg gaccgcacaa ttattacatt cccaaagaag aattaatgaa 1620 ataacattaa ctatccctgc cgacatctct gctactacag gtgctagaag tgccagtgtt 1680 ggttgggctg aaagtttgat aggtttacat ttgggtaaag ttgccttgat tactggtggt 1740 tccgcaggta taggtggtca aataggtaga ttattggctt tgtccggtgc aagagttatg 1800 ttagccgcaa gagatcctca caagttggaa caaattcaag ccacaattag agcagaatta 1860 gcagaagtcg gttataccga tgtcgaagaa agagttcaaa tcgctccagg ttgcgacgtt 1920 tcatccgaag aacaattagt tgacttagtt gaaagaacat tagcagcctt tggtacagtt 1980 gactatttga tcaacaatgc tggtatagct ggtgtagaag aaatggttat cgacatgcca 2040 gttgaaggtt ggagaaacac attgtatgcc aatttgatct caaactactc cttgatgaga 2100 aaattggcac ctttgatgaa gaaacaaggt tcaggttatg ttttgaatgt ttcaagttac 2160 ttcggtggtg aaaaggacgc agcaatccca tacccaaaca gagctgacta tgctgtctca 2220 aaagcaggtc aaagagcaat ggcagaagtt ttcgctagat ttttgggtcc agaaatccaa 2280 attaatgcta tagcacctgg tccagtagaa ggtgacagat taagaggtac aggtgaaaga 2340 cctggtttat ttgcaagaag agccagattg atcttggaaa acaaaagatt aaacgaatta 2400 catgccgcat tgatcacagc agcaagaaca gataatagac caatgagaga attggtagaa 2460 ttattattac ctaacgatgt tgcagcattg gcacaacatc ctgctgcccc agatgttttg 2520 agaaccttgg caaagagatt tcaatccgaa ggtgaccctg ctgcatcatc ctcttctttc 2580 ttattgaaca gaagtatcgc tgcaaaattg ttggcaagat taattaatgg tggttacgat 2640 ttgccagcag atatcttcgc taacttggca gtcccacctg atccattctt tacaagagcc 2700 caaatcgaca gagaagccag aaaagtcaga gatggtataa tgggtatgtt atatttgcaa 2760 agaatgccaa cagaatttga cgttgcaatg gctaccgtat actacttggc agacagaaat 2820 gtttccggtg aaaccttcca tccttctggt ggtttgagat acgaaagaac accaacaggt 2880 ggtgaattgt tcggtttacc tgctccagaa agattagccg aattggttgg ttccactgtc 2940 tatttgatcg gtgaacactt gactgaacac ttaaacttat tggcaagagc ttacttggaa 3000 agatatggtg ccagacaagt tgttatgatc gtagaaaccg aagcaggtgc cgaaaagatg 3060 agacacttat tacatgatca tgttgaagca ggtagattac caatcatagt agcaggtgac 3120 caaattgaag ctgcaatcga ccaagcaata gctaattatg gtagacctgg tccagttgtt 3180 tgtactccat tcagaccatt accttccgcc ccattagttg gtagaaagga ttcagactgg 3240 tccaccgtat tatcagaagc tgaatttgca gaattgtgtg aacatcaatt gactcatcat 3300 tttagagtcg caagaaagat agcattgtcc gatggtgcat cattggcctt agttacacct 3360 gaaactaccg caacatcttc aacagaacaa ttcgccttag ccaacttcgt caagacaact 3420 ttacatgcct tcaccgccac tatcggtgtc gaatcagaaa gaactgcaca aagaattttg 3480 atcaatcaag tagacttaac cagaagagca agagccgaag aaccaagaga tccaagagaa 3540 agacaacaag aattggaaag attcatcgaa gcagttttat tagttactgc tcctttacca 3600 cctgaagcag acactagata cgcaggtaga atacatagag gtagagccat cactgtttaa 3660 3660 <210> 39 <211> 3696 <212> DNA <213> HPDH-MCRct <400> 39 atgagaagaa caaaaccaga atgcttgaga agaaatgaaa gtacaatgag attgaacggt 60 aaagtcgcct taataacagg tgccgcaggt ggtatcggtt cagtagtcac aagaatgtac 120 ttgagagaag gtgctagagt tgcaatttcc tcaagatcct tggaaagagc agaacaattc 180 agagcagcct taatggccga aggtttttca ggtgacgata ttttgccagt agcatttgcc 240 agtacagact tagccggtat gcaaacagct atcgaagcaa ttgcagctag atggggtggt 300 ttggatgttt taattaacaa tgctggttca gctggtgcaa agcaaccttt gttcagaatc 360 cctttcacct cagaagattt ggctcaattg gcagctgaag gtttcacaga aaccgaaaca 420 atgagagaaa gtgctgccaa cttattgggt atgccttggc acttaacaag attggcttta 480 ccttacttga gagttggtgc ttctattatc aatgtatcta caatcttctc aagaaccaat 540 tattatggta gaataccata cgtagtccca aaatctgcct tgaacgcttt atccttgggt 600 ttggccaaac aattgggtac cactgacaga gctgtcagag ttaataccgt ttttccaggt 660 ccaatagatt cagacagaat cagaaccgtt ttcggtgcta tggacagatt gaaaggtgtc 720 ccagaaggta ctaccgctca agaatttttc gacattatga ttttggctag agcaagaggt 780 ggtgaagcac ctacaaagtg ttatccaact caagctgatg ttgctcacgt aattttattt 840 ttgggttcag atgaatctgc agccatatct ggtcataact ttgaaataac tcatggtatg 900 caagttaagg cacaatccag aacaaaattg actgcatggc cagaccaaag attgatagat 960 ttgtcaagaa gagttgtctt gattgttggt ggtgaacaag tacatgatgc tgtcacaata 1020 gcagcctccc aacaatcata tggtgcttca gttatcttga cattcagaca accagaagca 1080 gttaatactg caaaagctca attggaatcc agaggtttga accattctat agcagttacc 1140 tggttagacc cattgagaag agaatctgtt acaagtgttt tcgacttaat cagagaaaga 1200 taccacagat tggataccgt tatagttttt ccagctcatg ccgctggtca ttatggtcct 1260 gaattgatcc cagatgaacc tgaaagaatc agacaattca ccgatgaagc tattttaggt 1320 acaaccgcat tcgcagctca attgtccaga tttttattgg ctttcgatgc agaaaacgaa 1380 ttgcaagaac ctgtcaacgt tttgtttgtc acaaacgaac acgacggtgc tacaaatgca 1440 tttgaagata tctatagagc cgcagtcgaa caattgttga gagtttggag acacgaagat 1500 gaattgcaag ttgcagctgg tacaagaaga ttcgccgtaa gaaccaacca aatagtaaga 1560 tatgctaatg cagaaccaga caacttgaag tttacagccg attggttggc aacattagcc 1620 aacagagtca gaatctttga tgaattaaac ttatacgtcc ctgaaaacat acaaagaacc 1680 accggtaaaa ctgaattgcc aagagatatt gccagaactt tattaggttt gcacataggt 1740 aaagttgcag taataacagg tggttcagct ggtataggtg gtgctatagg tcattactta 1800 gctttgtcag gtgcaaaagt agttttggct gcaagagatg ctgaaagatt ggctgcattg 1860 aaaagagaaa tcgttgcaga attatttgcc gttggttacc ctcaaccaga aaccagagtt 1920 gaaaccttgg ctaatataga cgtagctgat gaagaagctt tgggtagatt agtaaaattc 1980 gctttggata aatttggtag aatagacttc ttgataaata acgcaggtat tgctggtgcc 2040 gaagaaatgg ttgtagactt gccattggca gcttggagac atactttgaa agcaaatttg 2100 ttaagtaatt actccttgat atacaagata gcacctttga tgaaaaagca aggtgcaggt 2160 tatatcttga atgtatcatc ctacttcggt ggtgaaaagt acgttgccgt tgcatatcca 2220 aatagatccg attacgctgt ttccaaagct ggtcaaagag cattggctga aatcttggct 2280 agacacttgg gtcctgaaat ccaaattaat gctttatccc caggtcctgt tgaaggtaaa 2340 agattaagag gtgaaggttc tagacctggt ttatacgcca gaagagcaag attgatcttg 2400 gaaaataaga gattgaatga catccatgat gctgttatca gagcatggag agaaggtgaa 2460 gctgttaaat ccaccttggg tgcattggct cataatcatt tcgaagatgt tttagctgca 2520 ggtccaccag aacctgttag aagattaatt acccatttga gagctgcagc tgtcaacgaa 2580 ggtcattcag attcccatta cttgatgacc agaaaaatcg ctgaaaagtt gttgcacaga 2640 ttgttcgaag gtggttactt gacatcagtc gaagatagag aagcttttac cttagctttc 2700 ttgagagaat taccagaacc tccagatcca tttttcgatc caaaagaaat tgatagagaa 2760 gctgaaagag ttcaatctgg tatcttgact atgttgaatt tgagaagaat gcctacagaa 2820 gaagaaatcg ctatcgctac agttttctac ttgtctgatc ctaacatttc tggtgaaacc 2880 ttgcacccat caggtggttt gaaattcgac agaacagtaa ccgaaggtga attttatggt 2940 ttaccaagaa atgaacattt gatggaatta agaagaaaga atgtattgtt aatcggtgac 3000 tacttggaag aagaattgag atggttggtc ggtgctttcg caatccaatt agttgtaaga 3060 aattgtatca ttttgacaaa gaccgccgaa agagctgata gtttgagaca ttacttttct 3120 gcacaaagat tgaacaattt gcatatcttc tctgctggtg caaacgttga agccgctatg 3180 gatgacttga taagaagatt tggtcacttg gatgtcgtag tatccacacc ttttgaccct 3240 ttaccattga aacctttggc tgcaactgat gattggagag gtgtcttgaa tgaaaaagac 3300 ttcgaacaat tggttgaaac acatattact catcacttca gagtcgccaa aaaggctgcc 3360 ttgcaagata aatcccaaat cgtattagta acccctaaga catcaagaaa tagttccaga 3420 gaagaatttg ctttagcatt gttcgttaaa acaaccttac atgcattgac agctaccttg 3480 gcagttgaat gcgaaagatt cacccatcat cctactgtaa atcaagttga tttgacaaga 3540 agagctagag tcgaagaacc aagaaacgaa agagaagaaa gagaagaatt ggaaagattc 3600 gtagacgctg ttttattggc tgccgcacca gctcctgaac cagaacaatc cagatacttg 3660 tccagaatac atagaggtaa cgccatcact gtttaa 3696 <210> 40 <211> 3651 <212> DNA <213> HPDH-MCRgb <400> 40 atgaacgata tgtccgatat tccacaagac ttattaacac aaagtatagc aggtagaaga 60 ttagataaca aatgtattat tttaactggt gcagctggtt caatcggttc ctttattaca 120 agacaattat tgagagaagg tgcaagagtt atgttaacag gtagagatca atcaaaattg 180 gacgacttta ttggtgactt ggtatcagaa ggtttcgaag aaactttcat ggtttccgca 240 actggtgact gtgccgatcc tgaagtctgt agagaaatag ttgccaagac agttgccgca 300 ttcggtccta ttgatgtttt agtcaacaat gccggtgccg caggtccaaa attcacattg 360 agagatatcc ctttcaccga agcagaacaa agagcagctg actcagatca aacaatgttt 420 gactcagcca tgaatttgtt gggtgctcct tggaatatgg ccagagccgc tgcaccacat 480 atgtcagctg gtgcctctat aatcaacgtt agtactatct tttctagaac tcattactat 540 ggtagaatac cttacgtcgt tccaaagtct ggtttgaacg cattgggtaa aggtttggca 600 ttagaattgg gtgaaaaaca aggtatcaga gtaaacactt tatttccagg tcctattgaa 660 tccgaaagaa ttgaatcagt tttcgccaga atggatgaat tgcaaggtgt tgaacctggt 720 tcaactggta aagagtttag agatttgatg ataactacaa gaacctctga tggtggtttg 780 gaatataaat acccaactcc aacagatgtt gcatcaggta tcgtttggtt ggcatctgat 840 gaatcctcag ccttatctgg tcatcacatt gaagtcacca atggtatgca agtaccagca 900 caatctagat cccaattggt ttcatggcca gataagagat tggaagattt gtcaggtcaa 960 gtcgttttga ttttaggtgg tgacgactat caagaagcag ttactttcgc cgaaagacaa 1020 atccaatccg gtgcaagagt aatgttagct ttcagaaact tgaataccgt tggtcatgct 1080 agatcattag ttcaatcaag aaatttgggt gacgttcaat tatcccactt ggaccctttg 1140 agaagagaat cagttgacag atccatgcaa ttaatcgcag atcactttgg tagattagat 1200 ggtgtaatct tgttaccaca aaagccaaac ggtgaatacg gttattcctt gactacagct 1260 aatgacgaag atgttgccaa ttttgttaga gatgaagttg tcgcccctgt tgcatttgca 1320 tccgctttcg caagaaacat ggatcaattg tttgccgaag gtgaaccacc agccatcgtt 1380 tacgttacta accctagtga tagacatggt aacttgatga atgaaattat cagagcaagt 1440 gttgaagcct taatcagagg ttggagacat gaagatgaaa ctttggcaaa cactggtgaa 1500 ttgccttggg cagtacaacc aaaccaaatg gttagatatg ataccgaaga tagagaagct 1560 ttaactttcg cagccgactg ggcagccact ttaaccaaca gagttagaaa gatggatagt 1620 atcaacttat ggttaccaag aagtattaaa agatccactg gtaaaagtag tatgccaagt 1680 agtataagta gagtattgcc tggtttgcac aagggtagaa cagccgtaat cactggtggt 1740 tccttaggta ttggtttaca attgggtaga tttttggcaa tagctggtgc cagagtcttg 1800 ttatctgcca gaagtgaagg taaattggcc gaagcaagag atgaaatcgt tgacgaattg 1860 agaaacatag gttatcctag accagaatct agagttacaa ttttgggtgg tatcgacgta 1920 ggtgaccctg atgcattgga tagattacac gatcatgccg ttgcagaatt gggtcacgta 1980 gatttcttga ttaataacgc tggtatcagt ggtgccgaag aaatggtagt cgatatgacc 2040 agagcagact gggatagaac tatggaagcc aacttgatct ctaactactc attgattaga 2100 aaattctcac ctgccatgaa agcaggtggt aaaggttcca tattgaatgt ttcctcatac 2160 tttggtggtg aaaagtatgt tgccgtagca tatccaaaca gagctgatta cgccgtatct 2220 aaggcaggtc aaagagtatt agcagaaata ttgtcaagac acttgggtcc tgaaatccaa 2280 atcaacgctt tagcacctgg tccagttgat ggtgccagat tgagaggttc agccgaagcc 2340 cctggtttat tcgatagaag aggtagatta gttttagaaa ataagagatt aaatgaagtc 2400 cataaggcta tcttggctgg tatgagttca ggttttgatg ctgacggtgt tatgagtttg 2460 gcatcaaacg ctgttgattc aattgacatc gactccttac ctaaaccagt cgccagattg 2520 gtcttgaaaa ttagagattc tggtggtatg ggtaactcct cacactactt aatgcacatg 2580 ggtatcgccg caaaattgat gactagatta gttagagcag gtatcttgtc cgacgaacaa 2640 agagaacaat ttttgggtac attcatagac gccccagcac catttttcga tttctcagaa 2700 acttccaagc aagcagaaca aatcgaaaca ggtattttga acagattaca cttgcataag 2760 atgcctacag atgaacaagt cggtttatcc accgtctttc acttggcaga tgacattgta 2820 tctggtgaaa cctttcatcc atcaggtggt ttgaaattcg acagatccgt cacagaaggt 2880 gaattgttgt tacctccaaa tgaaacagaa gttgctaagt taaagggtaa aagaatcgtt 2940 ttgattggtg actcaatgag aaacgaattg actcatatag ctaatggttt cttagcacaa 3000 aatgtagaaa aattgtgggt tttgactaag acagaagatt ccgcaaacgc tatgactcat 3060 tcagtcgata accctcatgg tatcgacata gaatgtagag ctattggtga cgacttagaa 3120 agttcattag atgacatctt gagaaacgat ggtggttatg acgttgtagt ttcatcccca 3180 ttcgaaagat tgccattaaa tgcattagct gccaacgcta atgaaccttg ggatagagtc 3240 ttatccgacc atgagtttag aaagttagtt cacgaccaat tgacccatca ctttagagtc 3300 gcaagaacca gtgccttggt tcctaattgc caaatcgttt taattactcc tgacacttct 3360 ttggcatcaa ctagagaaga atttgctttg gccttattcg ttaagaactc attgcacgct 3420 tttacagtca cattgggtgt agaaggtgaa agattgccaa cagttccagc aattaatcaa 3480 gtccaattga ccagaagagc acataccgaa gaaccatcca acgaccaaga attggaagaa 3540 gaaatgacaa gattagtcca tgccgtatta caatgctcag tccctgcacc agcaccatcc 3600 gattcaagat acttgtccaa aatctttaga ggtaacgctg tcaccgtata a 3651 <210> 41 <211> 3666 <212> DNA <213> HPDH-MCRot <400> 41 atgtttatga caagattgaa tgacaagatt gctttgatta ctggtggtgc tggtactata 60 ggtgaagtta ttacaagaag atatttggaa gaaggtgcaa cagtagttat ggctggtaga 120 aatagagaca aattagacag atatagagaa agattgataa ctgaatttca tgctttacca 180 gaaagagtta tggtagttag aatggacggt tcatccaacg cagaagttag aatgggtatt 240 gccgctgttg tagcccactt tggtagaatt gatatattgg taaataacgc aggttcagct 300 ggtgccagac aaagattgcc agctatccca ttattgagat cagaattgca agcagacgaa 360 accgaaactt tggcagattc aatcggtaac ttaataggta tcacatggaa tttgattaga 420 gctgcagctc cattcatgcc tgcaggttcc tcagttatca atattagtac aatctttgct 480 agaactgact actatggtag aatcccttat gttgtaccaa aggccgcatt acacgcctta 540 acattggcag ccgcaactga attaggtgaa agaggtataa gagttaacca aatcaaccca 600 ggtcctatcg actcagatag aattagaact gtttttagaa gaatggatga attgaagggt 660 gttccagaac aatcaactgc tgatggtttc tttcaaatga tgagattgag aaggcctaat 720 gccgaaggtg acttagttaa gggttttcct aagactttgg atgttgccaa cgttgctgta 780 tttttaggtt ccgcagaatc agctgcattg tccggtgaaa cattggatgt tacacacggt 840 atggcagttc caacagaatc aagaacaact ttgacaagta gacctggttt gagagcagta 900 gacggtagtg gtcacacaac attaatttgt gttggtgacc aaatcgaaga agcagcagcc 960 ttaactggtg tattgagagc atgtggtgcc gaagtagtta tcggttttag atctagagcc 1020 gcaatcgcta gattcgacca cttgatcgaa agaggtagac acttaccatc tcaagaacat 1080 gttgcccctg ttttgttata tttgaatcct acagaaccag aatcaataga ccaagccttg 1140 agatggatgg ccactaactt agacttgcct acaagtgtaa tcattttgcc tgcacaaaga 1200 caaccattac ctcctagtgt tgttagagcc tcagatgaag aagttgcata ctttttaaga 1260 gatgaattga gtggtatgat cgtcttggca agtagattag caagattctg gcaacaagca 1320 acattggcac ctggtaatgc acctattcaa ccaagagttt tattcatgac aaacccagac 1380 gatggtcaag gtaacttata tgcagaaata ttgagagcag gtgttgaaca attgtgtaga 1440 gtttggagac acgaatccca attggattat actagattgg cccaaatgga tgcacatcca 1500 cctcatatta gaccagtttg ggcaaaccaa ttggttagat ttgccaacaa tgaacaagaa 1560 aatttggaat actgctgtgc ttgggttgcc aagatattgt tatccgaaag aacaattgaa 1620 gaattaaact tatacttgcc tagacaaata ggtagtacaa caggttccag acaaccttcc 1680 tttggttggg cagaaaactt gataggtttg cacttgggta aaacagcatt gataactggt 1740 ggttcagctg gtataggttc ccaaatcgca agattgttag cattgtcagg tgcaagagtt 1800 atgttgtgcg caagagatga aagaaaatta atccaaatga gagacatgat aatcgcagaa 1860 ttgactgaag tcggttataa ccaagttgaa tctagagtac aaatttgtgc cggttgtgac 1920 gttggtgaag aagaacaatt ggaaattgct gttcaaagaa ctttggactt atttggtcat 1980 gttgattact taattaataa cgcaggtatt gccggtgctg aagaaatggt tttagacttg 2040 cctttagaag cttggcaaag aactttaaga acaaatttga taagtaatta ttcattgatc 2100 agaaaattgg ctccacaaat gaaatcaaga ggtagtggtt atatcttgaa tgtctcttcc 2160 tacttcggtg gtgaaaagta tgccgctatt ccttatccta atagagctga ttacgctgta 2220 tccaaagccg gtcaaagagc cttaggtgaa gctttggcta gattgttagg tccagaagtt 2280 caaatcaacg caatggctcc aggtcctgtt gaaggtgaaa gattgagagg ttctggtgac 2340 agacctggtt tatttttaag aagaggtaga ttaattttgg aaaacaaaag attaaacgac 2400 ttgcacgcaa cattgattgc tgcagaaaga gaaacccaag ttggtatgag agacttatta 2460 gccagattgt tacataacga tgtttgtgct ttgattgatg accctgcagc cccaacacac 2520 ttgagagctt tggctgaaag aatctgggaa caatccgatc caaactccta tgctagagcc 2580 ttctttatga atgccaatat tgctactaag ttattggcca gattgttcaa cgccgatcaa 2640 attgacgccc aaacattcca cacatcccaa cctaacttgc cacctgaacc tttcttcgct 2700 agaacccaaa tagatagaga agctagaaga gtcagagatg gtgtaatgtc catgttgtac 2760 ttacaaagaa tgcctacaga atttgacgta gcattagcca cagtttatta cttgaacgac 2820 agatccgttt ctggtgaaac attccatcca agtggtggtt taagacatga aagaacccca 2880 accggtgccg aattgtatgg tagtcctgca ccacaaagat tggccagttt agccggttca 2940 accgtctatt tgattggtga atcaatggct gctcacttgg aagctttagc aagagcttac 3000 attgaaagat acgcagctac aagagttgtc ttgatctgtg ctacacctgc aggtgttgaa 3060 agattcagtc atcacttagc cgaccactta gcttctggtg ctttggccat attatccgcc 3120 gaagaaggta tcgaagcagc tttgtcagaa gccttgagaa gattcggtcc tccaggtcca 3180 gttgttagta ctcctttcca acctttgcca tcacaacctt tgattggtag aaacgactca 3240 gattggagta ccgttttaga tgttgctggt ttctccgcaa tgtgtgaaca acaattaacc 3300 catcacttta gagtcactag aaaattaagt ttggtcgcag gtgtatcatt ggttttggtc 3360 actcctgaaa cagattcaca cagttccaca gaacaattcg ctttggctaa ctttgttaag 3420 actacattac acgctttcac tgccacagtt ggtgttgaat gcgaaagaac agcacacaga 3480 attttggtca atcaagttga tttgggtaga caagctagag cagaagaacc aagatcacct 3540 gcagaacaag cacaagaaat ggaaagattc attgacgcca taatgttaac taccgcacct 3600 attccagctg aagaagataa cagatatact ggtagaatat acagaggtag agccataaca 3660 gtttga 3666 <210> 42 <211> 3690 <212> DNA <213> HPDH-MCRrc <400> 42 atgtctacag tcagaagatt ggaaggtaaa gttgcattga taacaggtgg tgccggtaat 60 ataggtgaag taataacaag aagatttttg gcagaaggtg ctaccgttgt tatcacaggt 120 agaaatgccg aaaaattggc tgtctacaga agaagattaa ttgacgaaga aagagtagca 180 ccagaaagag tcgttgcttt gagaatggat ggttctgaca ttgcccaagt tagagctggt 240 gttgctcaaa ttgttcacgg tggtacagat gtcccaatcc cattgcatag aatagatatc 300 ttggtcaaca atgcaggttc tgcaggtcca agaagaagat tagttgatat ccctttggaa 360 ccatccgaag ttcaacctcc agattcagaa actttggcac aagctgttgg taatttggtt 420 ggtataacat ggaatttgac aagagcagcc gcaccacaca tgccatccgg ttcatctgtt 480 attaacatct ctacaatctt ctcaagaacc gattactacg gtagaatagc ctatgtagca 540 cctaaggctg ctttgaacgc attatctgac ggtttggcca gagaattggg tgttagaggt 600 atcagagtca ataccatata tcctggtcca atcgaatcag aaagaatcta cactatgttt 660 caagctatgg acgctttaaa gggtcaacca gaaggtgaca cagctagtgg tttcttaaga 720 atgatgagat tgtctagaat cgaccaaaac ggtgaagttg ttaagagatt cccaagtcct 780 gtagatgttg caaatactgc tgtattcttg gcatctgacg aaagtgcagc ttttacaggt 840 catgcctttg aagtcacaca cggtatggaa gttccaaccg aaagtagaac cactttcgtt 900 tccagaccag gtttaagatc cgttgatgca accggtaaag ttattttaat atgtgccggt 960 gaccaagttg atgacgctgt cgcattggct gatacattga gatcatgtag agctaccgtt 1020 gtcatcggtt tcagagatcc aagagcatta gaaaaggcat ccgttttgtt gagagaacct 1080 agacacgctt tggcagctga catgtacggt agacctacaa tgactgcaga agctagattg 1140 gtcagattag atcctttaga cccaagagca gctgcccaaa ctttggaaca aatacatgcc 1200 gaattgggtg ccatccacca tgctgtcgta ttgcctggtc aatctagaca tgcaccttca 1260 gcttccttga tcgaagttga tgaccaagtt gttgaaagat tcttacacca agaattagtt 1320 ggtactattg ccttagcaag agaattggcc agattttggg aagaatatcc atccggttct 1380 tcaatgcata gagttttatt tgtttccaat cctgacgatc aacaaggtaa ccaatacagt 1440 cacatcttga gagctgcagt tgaacaatta gtcagagtat ggagacacga atccgaatat 1500 gactccgtta accctgcaca ccaacaagaa ggtcaatcct cagcagctgt ttgggctaac 1560 caattaataa gatatgttaa taacgaaatg gctaacttgg acttcacttg cgcctgggtt 1620 gccaagttat taggttccga tagaagaatc gccgaaatta acttatactt gcctgaagaa 1680 atcgttggta caataggtgt ccacaatcct ggttttggtt gggctgaatc cttatttggt 1740 ttacacatgg gtaaagtcgc attaataaca ggtggttccg ccggtatcgg tggtcaaatt 1800 ggtagattgt tagcattaag tggtgctcat gttatgttag cagctagaaa tgcagatcaa 1860 ttagaacaaa tgagagcttc catagttaga gaagttagag atgcaagtta tcctgacgca 1920 gaatcaagag ttgccatctt ccctggttca gacgtttcag atatcgacgg tttggaaaga 1980 ttggtaaatc atacagtaag agtttttggt aaagttgact atttgatcaa caatgctggt 2040 atagcaggtg cagaagaaat ggttatagac atgccagttg atgcatggag acacacattg 2100 agagccaact taatctccaa ttatgcatta ttaagaagat tagcaccaca aatgaaggca 2160 gccggtggtg catacgtatt gaacgtatca tcctattttg gtggtgaaaa atacgttgcc 2220 ataccatacc ctaatagatc cgactacgca gtttccaaag ctggtcaaag agcaatggtt 2280 gaatccttgg caagattttt gggtccagaa atacaaatca acgcaatagc tcctggtcca 2340 gttgaaggtg aaagattgaa aggtgctggt tcaagacctg gtttattcat gagaagagcc 2400 agattgatct tagaaaataa gagattgaat gaagttttcg ccgctttatt ggccgcaaga 2460 catgaaggtg ccacaatagc cgacttgtta cctgacttgt tcgccaatga tatccaatca 2520 atagcaaatt ccgcagccat gccagcacct ttgagaagat tagccacaat gttgagagaa 2580 acatctgacg caggtggtag tgcccaatca tatttgatga acgctactat cgcaagaaaa 2640 ttattgaaca gattggaaaa cggtggttat atcaccttac acgatagaag agccttgact 2700 gttgaaccac ctgaaccttt cttcacagaa gcacaaatcg aaagagaagc catcaaagtt 2760 agagatggta tcttgggtat gttacactta caaagaatgc caaccgaatt tgatgtagca 2820 ttagcaaccg ttttctactt ggccgataga aatgtcacag gtgaaacttt tcatccatca 2880 ggtggtttga gatttgaaag aacagttacc gaaggtgaat tgttcggtaa accaggtcaa 2940 caaagattag aaagattgaa gggttctgta gtttacttaa tcggtgaaca tttgagacaa 3000 cacttggttt tattggccag aacctttttg gatgaaatac atgtagccag agttgtttta 3060 ttgaccgaaa ccacacaagc agctactgac ttagcagccg aattgtctga ctatgaagct 3120 gcaggtagat ttgtcgtaat cccaacttgc ggtgacatag aaggtggtat tgacagagca 3180 atggcagaat atggtagacc tggtccagtc atctcaacac ctttcagacc tttaccagat 3240 agagctttat cagctagaaa tggtgactgg tcctcagttt taactacagc tgaatttgaa 3300 gaattggttg aacaacaaat cacacatcac ttcagagttg caagaaaagc aggtttgatc 3360 gaaggtgcta acgttacctt agtcacccca cctacttcag caagatctac atccgaagaa 3420 tttgccttgg ctaattttgt aaaaacaaca ttacatgcat tgaccgccac agctggtgca 3480 gaatccgaaa gaacagttcc acatgtccca gtaaatcaag ttgatttgac cagaagagct 3540 agatccgaag aaccaagaac cccttccgaa gaagaagaag aattgcaaag atttgtcaat 3600 gccgttttgt taacctccgc tcctttacca acacctttgg aatcaagata tagagccaga 3660 atctacagag gtaatgctat cacagtttaa 3690 <210> 43 <211> 3615 <212> DNA <213> HPDH-MCRsl <400> 43 atgaatgcaa tgggtaaaac agaaacacaa ggtagattag ctggtaaagt agccttgata 60 actggtgccg ccggtaactt aggtggttct atcgtaagaa gatatttggc cgaaggtgca 120 acagttgtca tgtccggtag agatgctgcc agaactcaag cagctttaga tgcagcagta 180 gctgacgttg gtggtactgc ttcaattgtc actatggatg gtgcaaatat cgaatccgtt 240 agagcaggta tcgcagatgt tgtcgcaaga catggtagaa tagacgtctt ggttaataac 300 gcaggttcag ctggtccaaa gcaagttttg ggtgacttac ctttgttgcc agaagaattg 360 gaaggtacac ctgattcaga aacagttggt gacgcagcaa gaaatataat ggttgtcgca 420 tggaacttag ttagagctgc cgcatctgct atgggtgaag gtggttccat cattaatgtt 480 tccacaatat tctcaagaac tgattatttt ggtagagcag cttatacagt tccaaaagct 540 gcattgaata tcttatccaa aggtatcgca agagaattgg gtaccagagg tatcagagtc 600 aatcaattgt atcctggtcc aatcgcctca gaaagaatca gaacagtttt cgccgcaatg 660 gataagttga aaggtgacga tgccggttca actgccaacc atttctttaa tttgatggct 720 ttggaaagaa gtgtaggtga agatgccaaa gcaaagacct tccctacacc agctgatatc 780 gcaaacactt gcgttttctt gggttcagac gaatccgctg cctttgcagc acacgacttt 840 gaagttacac atggtatggc agtacctttg gaatcaaaat ccgaattttt gcatagacct 900 gacttgagag caatagatgg tgacggtcat ggtatcttga tttcagccgg taaccaatta 960 gacgatgcat tggctatggc aggtttgttc gcagcacaag gttcaaaagt cttgttgggt 1020 tttcacgcac aagctgcatt ggacgatgca gcagaaagat tgccagccgg tgtcaaagca 1080 gttagatttc caagacatga ccatatggca atggacgctg aattggccag attcacagca 1140 catgtagccg caattgacgg tgctattata ttgccaacaa gaggtccagg ttactttcat 1200 ggtgcattga acgaagcttc cgacgctgcc gttgaaagat ttgtcgatga cgaattggaa 1260 ggtgcaattg ctgttgcaag aacattagat agatattggc aacaaggtca agacttgcct 1320 ggtgacccta gagtcttgtt tatcacaaac cctgatgatg gtgcaggtaa tggttgggca 1380 gatgttggta gagcagcaat cgaagaattg ttgagagttt ggagagatga agctagagta 1440 gccgcaacac aaggtaaaac tcctagacct gtttggggta accaaattgt tagatttggt 1500 aataacgaac cagaaaatag agactttgct gcaggtcatg ccgcaagatt gttattaggt 1560 ggtagacaaa tagaaccaat caatttgtac atccctgcat ctatcacaaa agccactggt 1620 gccagaagag ccttagcagg taccgcagaa aatatcacag gtttgcattt gggtaaagta 1680 gccttgatta caggtggttc cgcaggtata ggtggtgcct tagccagatt gttagccttg 1740 gcaggtgcaa aagttatgat ggtcgctaga agagaatctg aattagatgc tgctagagaa 1800 agaatcgttg gtgaattgca agacatcggt ttcgcaggtg tcgaaagaag agttttgacc 1860 ttggctggta ttgatgttgc tgacatgaaa tcattaaagc acgctgttga tgaaacagtc 1920 agagcattcg gtagaatcga ctacttaatt aataacgccg gtgtatcagg tgcagaagaa 1980 atggctgttg atatgggtgt tgaaaacttc agacatacac aatttgcaaa tttgatctca 2040 aactacgctt taatgcacat ggttgtccca atgatgagaa aacaaggttc cggttatatc 2100 ttgaatgtta gttcttactt tggtggtgaa aagtatttgg ctgtctccta tccaaataga 2160 acagactacg cagtatcaaa gtccggtcaa agagcaatgg ttgaaagtat ggcaagacac 2220 ttaggtcctg aaattcaaat caatgccata gctccaggtc cagtagatgg tgacagatta 2280 gctggtttgg gtggtaaacc aggtttgttt gaaagaagag gtagattgat tttagaaaac 2340 aagagattga acgctgttca ctcagctgtt atcaagtccg tcagagcagg tggttctgtt 2400 gcccaaattt tattcagatt gtccagaaat gactcagttc aattgtccca cgataagtca 2460 aacccacaac ctttaagaga tttggccttg gcatgtgcaa gagaaggtga cgaatcatgc 2520 tgctgggaca gattcatctt gacacctgta ttggccgcaa agttgttgaa aagattaaga 2580 ttgggtggtt ggttgatagg taccgactgg caatccagag atgaatccga ttggttgtta 2640 agagtccctc cagacgacat gccttggttg ccagttgcac aaattggtag agaagcagcc 2700 aaagttagag gtggtgtatt gtcccaatta catttgggta aaatgcctac cgaaacagaa 2760 gttgctcaag caactgtttt ctttttagct gatagagcag tctcaggtga aactttcatg 2820 ccttcaggtg gtttaaatgt cgaaagatcc gccaccgaaa gagaattgtt tggttcccct 2880 aaggcagaaa gattggcaca aatgggtggt aaaacagttt ggttggtcgg tgaacatttg 2940 gcagactact tgggtgctgc agctcaaggt ttcttgaatt gtgacgttgc tagagtagtt 3000 ttaatcactg cctcagctgc aggtgctaca gccattaagg gtcatattgg ttccggtgct 3060 gacagagtag aaagtttatt gatagacggt gaaatcgaaa cagccttaga tgcagctttg 3120 gctacttggg gtcatcctac tacaatcgtt tccactccat tgaaagcatt gccagaccaa 3180 ttgtttgatg cagataatcc attagatggt gacggttttg ctgatgtcgt tgaacataat 3240 ttgactcatc actttagaat tgccagaaaa gtcagtttat ttgacgaatg ccaattggta 3300 ttagtttcac cagatgttcc tatgggtgaa ggtgcaaaag gtgcatttgc cttggccaac 3360 tttgtcaaaa ccacattaca cgcttttact gcaactatcg cagttgaaaa tgaaagattg 3420 gtccacgaag tcgccgttaa tcaaatcaac ttgacaagaa gagtaagatc agaagaacct 3480 aaaaacgcag ctgaacatga agaagaagtt agaagatttg caagagccgt tttattggct 3540 ggtgcaccat tacctgacgc agaagatagt agatatagat ccagaatcta cagaggtatg 3600 tccttaaccg tataa 3615 <210> 44 <211> 3663 <212> DNA <213> HPDH-MCRca_variant_3 <400> 44 atgagtggta caggtagatt ggctggtaaa atagcattga taacaggtgg tgcaggtaat 60 ataggttccg aattgacaag aagatttttg gctgaaggtg caactgttat catatcaggt 120 agaaatagag ctaaattaac tgctttggcc gaaagaatgc aagcagaagc aggtgttcca 180 gctaaaagaa tcgacttaga agtcatggac ggttccgatc ctgtagctgt tagagctggt 240 atagaagcta ttgttgcaag acacggtcaa atcgacatct tggtaaataa tgctggttct 300 gcaggtgctc aaagaagatt ggctgaaatc cctttgactg aagcagaatt gggtccaggt 360 gctgaagaaa ccttgcatgc ttctattgca aacttattgg gtatgggttg gcatttgatg 420 agaatagctg cacctcatat gcctgtaggt tctgccgtaa ttaacgtttc caccatattc 480 tcaagagcag aatactatgg tagaatccca tatgttaccc caaaagcagc cttgaatgca 540 ttgtcacaat tggctgccag agaattgggt gccagaggta tcagagttaa taccatcttt 600 ccaggtccta ttgaatccga tagaatcaga accgtatttc aaagaatgga ccaattgaag 660 ggtagacctg aaggtgacac agcacatcat ttcttgaata ccatgagatt gtgtagagcc 720 aatgaccaag gtgcattaga aagaagattt ccatcagtag gtgacgttgc agatgcagct 780 gtatttttag cttcagctga atctgccgca ttgtctggtg aaaccatcga agtaacacat 840 ggtatggaat tgccagcttg ttctgaaaca tctttgttgg caagaacaga tttgagaact 900 atagatgcat ctggtagaac aaccttaata tgtgctggtg accaaattga agaagttatg 960 gctttgactg gtatgttaag aacttgtggt tctgaagtta ttataggttt cagatccgct 1020 gcagctttgg cacaattcga acaagccgtt aatgaatcta gaagattggc aggtgctgat 1080 tttacccctc caatcgcttt accattggac ccaagagatc ctgctacaat tgatgcagtt 1140 tttgattggg gtgcaggtga aaatactggt ggtatccacg cagctgttat cttgcctgca 1200 acctctcatg aaccagcccc ttgtgttata gaagttgatg acgaaagagt cttgaacttc 1260 ttggctgatg aaatcacagg tactatcgta atcgcttcta gattggcaag atactggcaa 1320 tctcaaagat tgacaccagg tgccagagca agaggtccaa gagtaatttt cttgtcaaac 1380 ggtgctgatc aaaatggtaa tgtatatggt agaatacaat cagctgcaat aggtcaattg 1440 atcagagttt ggagacacga agctgaattg gattatcaaa gagcttctgc tgcaggtgac 1500 catgtattgc ctccagtttg ggctaatcaa atcgtaagat tcgcaaatag atctttagaa 1560 ggtttggaat ttgcctgtgc ttggaccgct caattgttac attctcaaag acacatcaat 1620 gaaatcacct tgaatatccc tgctaacatc tcagcaacca caggtgcaag atccgcttca 1680 gttggttggg ccgaatcttt aataggtttg cacttgggta aagttgcatt gatcacaggt 1740 ggttctgctg gtatcggtgg tcaaatcggt agattattgg cattgtcagg tgcaagagta 1800 atgttagcag atattcaaga agataagtta gaacaaatgc aagccatgat acaaagtgag 1860 ttagccgaag taggttacac tgacgtagaa gatagagtcc atatagcacc aggttgtgat 1920 gtctcctccg aagcccaatt agccgatttg gttgaaagaa ctttgtccgc tttcggtaca 1980 gttgattatt tgattaataa cgccggtatc gctggtgtag aagaaatggt tatagatatg 2040 ccagtcgaag gttggagaca tacattattc gcaaatttga tctccaacta cagtttgatg 2100 agaaagttgg ctcctttgat gaaaaagcaa ggttctggtt acatcttgaa tgtttcttca 2160 tactttggtg gtgaaaaaga tgctgcaatt ccatatccta acagagcaga ctacgccgta 2220 tcaaaggcag gtcaaagagc tatggctgaa gtttttgcta gattcttagg tccagaaatc 2280 caaattaatg caattgcccc aggtcctgtt gaaggtgaca gattgagagg tactggtgaa 2340 agacctggtt tgttcgctag aagagcaaga ttaatcttgg aaaataagag attaaacgaa 2400 ttgcatgccg ctttgatagc agccgctaga acagatgaaa gatctatgca cgaattagta 2460 gaattgttgt tgccaaatga cgttgcagcc ttggaacaaa acccagctgc acctactgct 2520 ttaagagaat tggcaagaag attcagatct gaaggtgacc cagctgcttc ttcttcttct 2580 gcattgttga atagatcaat tgcagccaag ttattggcta gattacataa cggtggttat 2640 gtattgccag ccgatatatt tgctaatttg ccaaacccac cagatccatt tttcacaaga 2700 gcacaaattg atagagaagc cagaaaagtt agagatggta taatgggcat gttgtacttg 2760 caaagaatgc caactgaatt tgatgttgct atggcaacag tctattactt agctgacaga 2820 aatgtttctg gtgaaacttt tcatccatca ggtggtttga gatatgaaag aactcctaca 2880 ggtggtgaat tattcggttt gccatcccct gaaagattag ctgaattggt cggtagtacc 2940 gtatacttaa ttggtgaaca tttgactgaa cacttgaatt tgttggccag agcttatttg 3000 gaaagatacg gtgcaagaca agttgtcatg attgttgaaa ccgaaactgg tgctgaaacc 3060 atgagaagat tattgcatga tcacgtcgaa gccggtagat tgatgactat tgtagctggt 3120 gaccaaatag aagctgcaat cgaccaagct attacaagat atggtagacc aggtcctgta 3180 gtttgtacac cttttagacc attacctacc gtccctttgg taggcagaaa agattctgac 3240 tggtcaaccg ttttatctga agccgaattt gctgaattat gcgaacatca attgactcat 3300 cacttcagag ttgcaagaaa gattgccttg tccgatggtg caagtttagc cttggtcacc 3360 ccagaaacta cagcaacttc caccactgaa caattcgcat tggccaactt catcaagaca 3420 accttgcacg ctttcacagc aaccatcggt gtcgaaagtg aaagaactgc tcaaagaatc 3480 ttaattaacc aagtagattt gacaagaaga gctagagcag aagaaccaag agatccacat 3540 gaaagacaac aagaattaga aagattcatt gaagctgttt tattggtcac agcaccattg 3600 ccacctgaag ccgataccag atacgctggt agaatacata gaggtagagc tataacagtt 3660 taa 3663 <210> 45 <211> 3663 <212> DNA <213> HPDH-MCRca_variant_6 <400> 45 atgagtggta caggtagatt ggctggtaaa atagcattga taacaggtgg tgcaggtaat 60 ataggttccg aattgacaag aagatttttg gctgaaggtg caactgttat catatcaggt 120 agaaatagag ctaaattaac tgctttggcc gaaagaatgc aagcagaagc aggtgttcca 180 gctaaaagaa tcgacttaga agtcatggac ggttccgatc ctgtagctgt tagagctggt 240 atagaagcta ttgttgcaag acacggtcaa atcgacatct tggtaaataa tgctggttct 300 gcaggtgctc aaagaagatt ggctgaaatc cctttgactg aagcagaatt gggtccaggt 360 gctgaagaaa ccttgcatgc ttctattgca aacttattgg gtatgggttg gcatttgatg 420 agaatagctg cacctcatat gcctgtaggt tctgccgtaa ttaacgtttc caccatattc 480 tcaagagcag aatactatgg tagaatccca tatgttaccc caaaagcagc cttgaatgca 540 ttgtcacaat tggctgccag agaattgggt gccagaggta tcagagttaa taccatcttt 600 ccaggtccta ttgaatccga tagaatcaga accgtatttc aaagaatgga ccaattgaag 660 ggtagacctg aaggtgacac agcacatcat ttcttgaata ccatgagatt gtgtagagcc 720 aatgaccaag gtgcattaga aagaagattt ccatcagtag gtgacgttgc agatgcagct 780 gtatttttag cttcagctga atctgccgca ttgtctggtg aaaccatcga agtaacacat 840 ggtatggaat tgccagcttg ttctgaaaca tctttgttgg caagaacaga tttgagaact 900 atagatgcat ctggtagaac aaccttaata tgtgctggtg accaaattga agaagttatg 960 gctttgactg gtatgttaag aacttgtggt tctgaagtta ttataggttt cagatccgct 1020 gcagctttgg cacaattcga acaagccgtt aatgaatcta gaagattggc aggtgctgat 1080 tttacccctc caatcgcttt accattggac ccaagagatc ctgctacaat tgatgcagtt 1140 tttgattggg gtgcaggtga aaatactggt ggtatccacg cagctgttat cttgcctgca 1200 acctctcatg aaccagcccc ttgtgttata gaagttgatg acgaaagagt cttgaacttc 1260 ttggctgatg aaatcacagg tactatcgta atcgcttcta gattggcaag atactggcaa 1320 tctcaaagat tgacaccagg tgccagagca agaggtccaa gagtaatttt cttgtcaaac 1380 ggtgctgatc aaaatggtaa tgtatatggt agaatacaat cagctgcaat aggtcaattg 1440 atcagagttt ggagacacga agctgaattg gattatcaaa gagcttctgc tgcaggtgac 1500 catgtattgc ctccagtttg ggctaatcaa atcgtaagat tcgcaaatag atctttagaa 1560 ggtttggaat ttgcctgtgc ttggaccgct caattgttac attctcaaag acacatcaat 1620 gaaatcacct tgaatatccc tgctaacatc tcagcaacca caggtgcaag atccgcttca 1680 gttggttggg ccgaatcttt aataggtttg cacttgggta aagttgcatt gatcacaggt 1740 ggttctgctg gtatcggtgg tcaaatcggt agattattgg cattgtcagg tgcaagagta 1800 atgttagcag atgttgatac tagaaagtta gaacaaatgc aagccatgat acaaagtgag 1860 ttagccgaag taggttacac tgacgtagaa gatagagtcc atatagcacc aggttgtgat 1920 gtctcctccg aagcccaatt agccgatttg gttgaaagaa ctttgtccgc tttcggtaca 1980 gttgattatt tgattaataa cgccggtatc gctggtgtag aagaaatggt tatagatatg 2040 ccagtcgaag gttggagaca tacattattc gcaaatttga tctccaacta cagtttgatg 2100 agaaagttgg ctcctttgat gaaaaagcaa ggttctggtt acatcttgaa tgtttcttca 2160 tactttggtg gtgaaaaaga tgctgcaatt ccatatccta acagagcaga ctacgccgta 2220 tcaaaggcag gtcaaagagc tatggctgaa gtttttgcta gattcttagg tccagaaatc 2280 caaattaatg caattgcccc aggtcctgtt gaaggtgaca gattgagagg tactggtgaa 2340 agaggtggtt tgttcggtag aagagcaaga ttaatcttgg aaaataagag attaaacgaa 2400 ttgcatgccg ctttgatagc agccgctaga acagatgaaa gatctatgca cgaattagta 2460 gaattgttgt tgccaaatga cgttgcagcc ttggaacaaa acccagctgc acctactgct 2520 ttaagagaat tggcaagaag attcagatct gaaggtgacc cagctgcttc ttcttcttct 2580 gcattgttga atagatcaat tgcagccaag ttattggcta gattacataa cggtggttat 2640 gtattgccag ccgatatatt tgctaatttg ccaaacccac cagatccatt tttcacaaga 2700 gcacaaattg atagagaagc cagaaaagtt agagatggta taatgggcat gttgtacttg 2760 caaagaatgc caactgaatt tgatgttgct atggcaacag tctattactt agctgacaga 2820 aatgtttctg gtgaaacttt tcatccatca ggtggtttga gatatgaaag aactcctaca 2880 ggtggtgaat tattcggttt gccatcccct gaaagattag ctgaattggt cggtagtacc 2940 gtatacttaa ttggtgaaca tttgactgaa cacttgaatt tgttggccag agcttatttg 3000 gaaagatacg gtgcaagaca agttgtcatg attgttgaaa ccgaaactgg tgctgaaacc 3060 atgagaagat tattgcatga tcacgtcgaa gccggtagat tgatgactat tgtagctggt 3120 gaccaaatag aagctgcaat cgaccaagct attacaagat atggtagacc aggtcctgta 3180 gtttgtacac cttttagacc attacctacc gtccctttgg taggcagaaa agattctgac 3240 tggtcaaccg ttttatctga agccgaattt gctgaattat gcgaacatca attgactcat 3300 cacttcagag ttgcaagaaa gattgccttg tccgatggtg caagtttagc cttggtcacc 3360 ccagaaacta cagcaacttc caccactgaa caattcgcat tggccaactt catcaagaca 3420 accttgcacg ctttcacagc aaccatcggt gtcgaaagtg aaagaactgc tcaaagaatc 3480 ttaattaacc aagtagattt gacaagaaga gctagagcag aagaaccaag agatccacat 3540 gaaagacaac aagaattaga aagattcatt gaagctgttt tattggtcac agcaccattg 3600 ccacctgaag ccgataccag atacgctggt agaatacata gaggtagagc tataacagtt 3660 taa 3663 <210> 46 <211> 777 <212> DNA <213> BDHcm <400> 46 atggtcaact tacaaggtaa aacagcattg gtcactggtt caacatcagg tatcggtttg 60 ggtatcgcag aagcattggg tagagccggt gctaatatag tattaaacgg ttttggtgac 120 gttgatgctg cattggcaac aatcgccgct accggtgcac aatctgccca tcacccagct 180 gatatgagaa aacctgacga aattgaagca atgatagcta tggcaagaga aagattcggt 240 accgttgatg tcttagtaaa taacgctggt attcaacatg ttgcacaagt cgaagatttt 300 ccagcacaaa agtgggatga catcttggcc ataaatttga cttcttcatt ccatactaca 360 agacacgtta tacctgctat gagagcaaga aattggggta gaatcgttaa cattgcttca 420 gtacatggtt tagttggttc cgccggtaaa agtgcttatg ttgcagccaa gcacggttta 480 gtcggtttga ccaaagtaac tgcattggaa acagccggta ccggtatcac ttgtaatgct 540 atttgcccag gttttgtttt aacacctttg gtcgctgcac aaatagatgc tattgctaaa 600 agagatggta tttctatgga agccgctaga gctagattgt tatctgataa gcaaccatca 660 ggtcaatttg tcactcctga acaattgggt aacttggttt taatgttgtg tagtccattc 720 ggtgaccaag ttagaggtgc agcctgggct atggatggtg gttggacagc tcaatga 777 <210> 47 <211> 771 <212> DNA <213> BDHkp <400> 47 atgaatttgc atggtaaaac agcattagta actggttcta catcaggtat cggtttaggt 60 atcgccaagg tcttagcaca agcaggtgca caattggtat taaatggttt tggtgactct 120 tctcatgcta gagcagaagt tgctgcattg ggtaaaatac caggttatca cgatgctgac 180 ttaagagatg ttggtcaaat tgaagctatg atgcattacg cagaatccac cttcggtggt 240 gtagatatcg ttattaataa cgccggtatt caacacgtcg ctccagtaga acaatttcct 300 gtcgataagt ggaatgacat attggctatc aacttatcca gtgttttcca tactacaaga 360 ttggcattac ctggtatgag acaaagaaac tggggtagaa ttataaacat tgcatctgtt 420 cacggtttgg tcgcatctaa agaaaagtca gcctatgttg ccgctaaaca tgccgttgtc 480 ggtttgacca agactgtcgc attagaaaca gccagatcag gtatcacctg taatgctatt 540 tgcccaggtt gggtattgac tcctttagtt caacaacaaa ttgataagag aatagccgaa 600 ggtgttgacc cagaacaagc ctccgctcaa ttgttagctg aaaagcaacc aagtggtgaa 660 tttgttacac ctcaacaatt aggtgaaatg gcattgttct tatgttctga tgcagccgct 720 caagtcagag gtgcagcctg gaacatggac ggtggttggg tagctcaatg a 771 <210> 48 <211> 1113 <212> DNA <213> HBDHos <400> 48 atgcaattat tcaagttaaa gaccagatta tcaaagttcg atcatttcgc agatttcgcc 60 agagagttta agttatcaga acatgacttg gttatcacta acgaattttt gtacgaacct 120 ttcatgaagt cattaaactt gccatgtcat ttcattatgc aagaaaaatt tggtttaggt 180 gaaccatcag acaagatgat gaacacaatt ttgaaagaag ttaagggtgt tggtttcgat 240 agagtaatag ctgttggtgg tggtactgtt atcgacattt caaaattgtt cgttttaaag 300 ggtgttgaag atgtcacaga agcattcgaa agaaaaattc caatcgtaaa agaaaagcaa 360 ttagttatct tgcctacaac ttgtggtact ggttcagaag ttacaaatat ctcaatagca 420 gaaatcacat caaagcatac caagatgggt ttggcagacg atgccatcgt tgcagatgac 480 gctgtattgg ttccagaatt gttaaaaggt ttacctttta agttttatgc ctgttctgct 540 attgacgcat tggttcatgc taccgaatca tatgtttctc caaaatcaaa tccatacact 600 agattatatt gtagagctgc cattgaaata atcattgatg tttttcaagg tatcgctgca 660 aacggtccag aatacagatt tgaaagattg ggtgacatgt taatggctag taactacgct 720 ggtatagctt ttggtaacac tggtgttggt gcagttcatg ccttgtcata cccattaggt 780 ggttcctacc atgttccaca tggtgaatcc aactaccaat tctttacaga agtttttaag 840 ttatataatg ccaagaaccc atcaggtgac atagctagat tgaatgactt gttatgtgta 900 caattgggtg ttaatgaaaa cccttaccca gttttagacg aattgttggg taaattgatt 960 cctaaaaaca aattgagagt ctacggtatg aagccagaag aaattcaagg tttcacatcc 1020 tcagttttac aaacccaaca aagattgtta gcaaacaact atgtatcctt atccgaagaa 1080 gaaatcagag aaatctacaa agtattatat tga 1113 <210> 49 <211> 1137 <212> DNA <213> HBDHps <400> 49 atggccttca tctattacgt cacacaaatc caatttgaat ttggtgccgt caagttatta 60 aagcaagaat gcgaaagagt aggtatcact agaccattga ttgtcacaga tcctggtgta 120 aaagctgcag gtatattaca aaaggcttta gatgcattgc caggtatcgc tgtaactgtt 180 tttgaccaaa caccatcaaa tcctaccgaa gccgctgtta gagcagccgc tgcattgtat 240 aaagcaggtg gttgtgatgg tttaattgct gttggtggtg gttccgcaat agactgcgcc 300 aagggtgttg ctatcgccgc tacacatgaa ggtcctttga ctacatacgc taccatagaa 360 ggtggttcac caaaaatctc cgatagagtt gcaccattaa ttgccgtccc taccacttct 420 ggtacaggtt ctgaagtagc cagaggtgct ataatcatcg ttgatgacca tagaaagttg 480 ggttttcatt catggcactt agttccaaag accgcaattt gtgatcctga attgacttta 540 ggtttgccag ctatgttgac agcagccacc ggtatggacg ccattgctca ttgcatggaa 600 acctttatgt ctccagcttt caatccacct gcagatggta ttggtttaga cggtttggaa 660 agaggttggt cacacataga aagagctact agagatggtt ccgacagaga agcaagatta 720 aacttgatgt ctgcttcaat gcaaggtgca atggccttcc aaaaaggttt aggttgtgtc 780 cattccttaa gtcactcttt gggtggtgta gatcctagat tacatcacgg tacattgaat 840 gccatgtttt taccagctgt tgtcagattc aacgctagtg cagaatctgt tcaaaaggaa 900 agaagattgg atagaatggc cagagctatg ggtttaagat caggttccga tatccctgac 960 gcaattaagg atatgaatgc cagattaggt ttgccaactg gtttggctgc aatgggtgtt 1020 caaagagatt ggttcgacag aattatagtc ggtgcattgg ccgatcattg ccacaagact 1080 aacccaagat tagctagtcc tgaagaatat agagaaatgt tagaaacatc tttgtga 1137 <210> 50 <211> 867 <212> DNA <213> HIBADHas <400> 50 atgcaaacta tcggtttcat cggtttaggt gttatgggta aatctatggc aagacactta 60 ttgagagcag gttattcatt ggtcgtttat accagaacta agcaaaaggc agaagatata 120 ttggctgaag gtgcattgtg gaaagaaact gtaaaggatg ttgctaaagc tgcagacgtt 180 gtcatgacaa tggttggtga accacaagat gtcgaatatg tatactttgg tgacgatggt 240 atcttggctc atgcaaagag aggtacatac gtcattgatt tcactacatc caaaccaagt 300 ttagctgtaa gaatacacga agccgctaaa gaaaagggta tctttgtttt ggatgcacct 360 gtctctggtg gtgacattgg tgctagagat ggtaccttat caataatggt tggtggtgac 420 aaggaagcct tctctgcttg tatgccattg ttgtcacatt tgggtaaaaa tatagtctgg 480 caaggtgaag ccggtgctgg tcaacacaca aagatgtgta atcaaattgc aatagccacc 540 aacatgatag gtgtatgcga agcattagtt tatgcagaaa aagccggttt agatcctgaa 600 agagttttga gatccattag tcaaggtgca gcctcttcat ggtctttatc aaacttggct 660 ccaagaatgt tggctggtga ctttgcacct ggtttctacg ttaagcattt catcaaggac 720 atgggtattg ctttagcaga agccgaaacc ttgggtttat ccttgcctgg tttaaaattg 780 gccaagcaat tatacgaatc cttggctgaa agaggtgaag aacaaagtgg tactcaagct 840 ttgtacaagt ggtacaagga atggtga 867 <210> 51 <211> 891 <212> DNA <213> HIBADHbc <400> 51 atggaaatcg cattcatcgg tttgggtaac atgggtggtc ctatggctgc taacttatta 60 aaggctggtc acgcattgac tgtattcgac ttagatgcac atgctgttga tgctgcagtt 120 agagctggtg ctgcagcagc aacttcacca agagatgctg cagccagagg ttctttaatc 180 attacaatgt tgcctgccgc acaacatgtt agagccgttt atttgggtga cgatggtgtt 240 ttagccggtg caagagccgg tgcaaccttg atcgactgtt ccacaattga ccctggtaca 300 gtcagagctg ttgcagatgc cgctgcacaa agagacttcc ctttggctga tgcaccagtt 360 tctggtggta caggtggtgc tcaagcaggt actttgacat tcatggttgg tgctgatggt 420 gcattgtttg aaagaattag acctgtttta ttggacatgg gtagaaatgt agttcactgt 480 ggtggtactg gtacaggtca aatcgctaag atctgtaaca atttgttgtt aggtatttca 540 atgatgggtg tttccgaagc aatggctttg ggtgcagcct tgggtatcga cccaaccgtt 600 ttagcaggta taatcaacac ctctacaggt agatgttggt cctctgatgc atacaaccca 660 tatcctggtg tttcagacac agcaccagct gcaagaggtt atgcaggtgg ttttgcagcc 720 aacttgatgt taaaagactt gggtttagca actgaagctg caagatcagc ccatcaacca 780 gtttggatgg gtgctttagc acaacaattg taccaaagta tgtcccaaca aggtttaggt 840 acattagact tttccgcctg cgtcaaatta tacgaagccc aaccagcatg a 891 <210> 52 <211> 888 <212> DNA <213> HIBADHma <400> 52 atggctaaaa taacattcat tggtttgggt aacatgggtg gtcctatggc tggtaacttg 60 gtaaaagcag gtcacgaagt cacagtattt gatttgtcca aagacgctgt cgctgcatta 120 gtaagtgaag gtgcaaagac cgccggtact gctcatgaag ccgcttcagg tgcagaatgt 180 gttataacta tgttgccagc aggtcaacac gttgaagccg tctatttggg tgacgatggt 240 ttgttagcaa aattgccagc cggtacatta gttattgatt cttcaacaat agctcctgaa 300 accgcaagag gtgtagctga agttgcaaga gccaaagata ttccattttt ggacgctcct 360 gtttctggtg gtgtcggtgg tgctaaggca ggtactttaa cttttatttg tggtggttca 420 gaagaagctt ttaataaggc aaagcctatc ttggatacta tgggtaaaaa tatcttccat 480 gctggtgacc acggttctgg tcaagttgca aagatatgca ataacatgtt gttagccatc 540 ttaatgtccg gtacaagtga agccttggct ttaggtgtca aaaatggttt agatccagct 600 gttttatccg aaattatgaa gcaatccagt ggtggtaatt gggcattgaa tgtttataac 660 ccttggcctg gtgtaatgga aggtgttcct gcttctagaa attacgaagg tggtttcttg 720 gttaacttaa tgactaagga tttgggttta gccttcgaca atgctgtcaa gaaccaagct 780 tctatcccaa tgggttcatt agcaagaaat ttgttccaat tgcatgccgg tcagggtaac 840 ggttctttgg atttctcttc aattcaaaga ttatacaaac ctgaatga 888 <210> 53 <211> 897 <212> DNA <213> HIBADHpa <400> 53 atgacagata ttgctttctt gggtttgggt aacatgggtg gtcctatggc tgctaactta 60 ttgaaggctg gtcacagagt aaatgtattt gatttgcaac caaaagctgt attgggttta 120 gttgaacaag gtgcacaagg tgccgactct gctttacaat gttgcgaagg tgccgaagtt 180 gtcatttcta tgttgcctgc aggtcaacat gtcgaatcat tgtatttggg tgacgatggt 240 ttgttagcaa gagtagccgg taaaccattg ttgattgatt gttcaactat agctcctgaa 300 acagcaagaa aagttgccga agctgcagcc gctaagggtt tgaccttgtt agacgctcca 360 gtctccggtg gtgtaggtgg tgcaagagcc ggtactttaa gttttatagt aggtggtcca 420 gctgaaggtt tcgctagagc aagacctgtt ttggaaaata tgggtagaaa catctttcat 480 gcaggagatc atggtgctgg tcaagttgca aagatctgca acaacatgtt gttgggtatt 540 ttaatggctg gtacagccga agctttggca ttaggtgtta aaaatggttt agacccagct 600 gttttgtccg aagtcatgaa gcaatcttca ggtggtaact gggcattgaa tttgtataac 660 ccttggcctg gtgttatgcc acaagcccct gcttctaatg gttacgctgg tggtttccaa 720 gtcagattga tgaataagga tttgggttta gcattggcca acgctcaagc agttcaagct 780 tctacccctt taggtgcctt ggctagaaat ttgttttctt tgcatgcaca agccgatgct 840 gaacacgaag gtttagactt ctccagtata caaaagttgt atagaggtaa agattaa 897 <210> 54 <211> 888 <212> DNA <213> HIBADHxc <400> 54 atgtctaaaa tcgccttcat cggtttgggt aatatgggtg gtcctatggt cgctaacttg 60 ataaaagcag gtcacgaatt gagagtattt gatttggttc attctgcttt agacactgca 120 cacgctgcag gtgctcaagt cgcatcttca gcctcagata cattacatgg tgcagaagtt 180 gtcatttcta tgttgccagc ttcaaaacac gttgaaggtt tgtatttggg tgacgctggt 240 attttagctc atataccagc aggtgccttg gttatcgact gttccaccat tgctcctgca 300 atggccagaa ccgttgcaac tgccgctcac gccagaggtt tggatatgtt agacgcacca 360 gtttctggtg gtacagctgg tgcagccgct ggtactttaa cttttattgt aggtggtgca 420 gcccaagcat tggaaagagc cagacctgtt ttacaagcaa tgggtaaaaa cttgttccat 480 gttggtgaca atggtgctgg tcaagtcgca aagttgtgta ataacatggc tttaggtgtc 540 ataatggctg caactggtga agctatcgca ttaggtgtag cccaaggttt ggacccagct 600 gtattatcac aaatgatggc agtttctaca ggtagatcat gggctaccga agtctgcaac 660 ccttggcctg gtgtattgga aaatgcccct gcttccagag gttatagtgg tggttttggt 720 aacgatttga tgttaaagga cttgggttta gttgctgaag ccgctgtcca agcaggtgcc 780 tccattcctt tgggtgaatt agctagaaat ttgtacgcaa tgaacagtag agccggtaat 840 ggtgctttag atttctccag tgtagttaaa ttggtcgcta aggtatga 888 <210> 55 <211> 762 <212> DNA <213> HPDHam <400> 55 atgacacaac aaacagtatt cgttacaggt gcctccgcag gtttcggtgc cgccatagca 60 agaagattcg tagcagaagg tgccagagtt attgccgtcg ctagatctga agataaattg 120 gaaaagttag ccggtgaatt gggtgacgct gtattgccag ttaaattaga tgtctcagac 180 cctgaagcag taaaggaagt tgtcacaaat ttgccagccg attggcaaca agttgatgta 240 ttagttaaca acgctggttt ggcaaaaggt ttagaacctg cccataaggc tgatttgcaa 300 gattgggacg aaatgatagc taccaatgtt agaggtttaa cccatgtcac tagagcattg 360 ttaccaggta tggttgaaag aggtagaggt cacgttatta atataggttc tatcgctggt 420 acttatccat accctggtgg taacgtttat ggtgcaacaa aagcctttgt tcatcaattc 480 tccttgaatt tgagaagtga tttgcacggt acaggtgtca gagtaaccaa cgtagaacct 540 ggtatggttg gtggtaccga tttttccaag gttagattcg atggtgacca agcaaaagcc 600 gataaggttt acgaaggtac tacaccatta actgctgaag atgtcgcaga aagtgtattt 660 tgggctgcaa atcaacctgc acacgttaat atcaacgtca ttgaattgat gccagtagtt 720 caatcttttt cacctttgca aatattcaga gataactcat ga 762 <210> 56 <211> 1653 <212> DNA <213> HPDHbs <400> 56 atgaccgctg gtaaaggtaa aggtgctaaa gttgaccctg ctggtggtaa agccgctact 60 ggtagattgg ccggtaaagt tgcattggtc acaggtgcag ctggtaattt gggtggtgcc 120 attattagaa gattcattgc cgaaggtgca actttggtta tgactggtag aactaaggaa 180 agattagctg cagcaaagca agctttattg gacgaaactg gtgttgaaga agcaagagtt 240 tttaccgtaa tcttggatgg tgctgatcca gattcagtta gagcaggtat tgatcaagtc 300 agagctgcct taggtagaat tgatgttttg gtcaataatg caggttcagc tggtcctaga 360 caaacattgg aaaacgtacc attgactaga gccgaaatgg aagcaaatgg tgactcagaa 420 acagtagctg acgccatgag aaatttgttg ggtgtcagtt ggaacttaac aagagcagct 480 gccccaataa tgccagtagg tgcatcagtc gttaatattt ccaccatctt ctctcacaca 540 agatactatg gtagaacagc ctatgtcgtt cctaaggctg cattaaacgc cttatcccaa 600 gaattagcaa aggaattagg tgcatctggt ataagagtaa atacagtttt cccaggtcca 660 atcgaaagtg aaagaattag aacagttttc gcagctatgg atgccttgca aggtgaacca 720 gaaaactcta ctgcaaatca tttcatgggt agaatgacat tggaaagagc tatcggtgac 780 gcccctaaaa gaaagacttt gccagttcca ggtgacattg cagatgcttg tttgttttta 840 gcatcagatg aaagtgctgc cttaaatggt aacgaaatag atgttacaca tggtatgcaa 900 gttagaaaag atagtagatc aacttacatg actagaccat ccatgagatc tttggacggt 960 gcaggtttga ccgtcttaat tgccgctggt gaagcatggg aagatgcatt agatatggct 1020 aagatccaag ctgcatgtgg tgccagagtc ttattgggta tgactagaca agttgacgtc 1080 gcacaagccg aagccagagt taaagctcaa ggtgctgaag aaggtttaac catcatgaga 1140 ttcaacagag ctgaacctga tggtatggaa gcagccataa gagcctattc tgatgcacat 1200 ggtgcaatcc actctgccat cgttttacct gtaaagccag ctggtcattt cggtgactct 1260 ttggttgaag cttcagatgc agaagttgac gctttgattg atatcgaatt aaccggtgct 1320 attgcagttg ccagaacctt gagaagatac tggaaaacat gctctgactt gatgcaagat 1380 ccaagattcg tctttatgtc aggtccatct gatggtaaag gtaacaaagc aggtagaata 1440 ttagcagccg caatagccca attaatcaca atttggagag atgaatctag agtagacttt 1500 gcacaaggta gacaaaagag agccgcatgg ggtaatcaaa tagttagata cacaaatgaa 1560 gaagatgaaa acaccagatt cgccgcaggt catgctacta gaattgtttt caaggaacaa 1620 aagataactg aaacttcctt gtacttgcct taa 1653 <210> 57 <211> 1653 <212> DNA <213> HPDHca <400> 57 atgagtggta caggtagatt ggctggtaaa atagcattga taacaggtgg tgcaggtaat 60 ataggttccg aattgacaag aagatttttg gctgaaggtg caactgttat catatcaggt 120 agaaatagag ctaaattaac tgctttggcc gaaagaatgc aagcagaagc aggtgttcca 180 gctaaaagaa tcgacttaga agtcatggac ggttccgatc ctgtagctgt tagagctggt 240 atagaagcta ttgttgcaag acacggtcaa atcgacatct tggtaaataa tgctggttct 300 gcaggtgctc aaagaagatt ggctgaaatc cctttgactg aagcagaatt gggtccaggt 360 gctgaagaaa ccttgcatgc ttctattgca aacttattgg gtatgggttg gcatttgatg 420 agaatagctg cacctcatat gcctgtaggt tctgccgtaa ttaacgtttc caccatattc 480 tcaagagcag aatactatgg tagaatccca tatgttaccc caaaagcagc cttgaatgca 540 ttgtcacaat tggctgccag agaattgggt gccagaggta tcagagttaa taccatcttt 600 ccaggtccta ttgaatccga tagaatcaga accgtatttc aaagaatgga ccaattgaag 660 ggtagacctg aaggtgacac agcacatcat ttcttgaata ccatgagatt gtgtagagcc 720 aatgaccaag gtgcattaga aagaagattt ccatcagtag gtgacgttgc agatgcagct 780 gtatttttag cttcagctga atctgccgca ttgtctggtg aaaccatcga agtaacacat 840 ggtatggaat tgccagcttg ttctgaaaca tctttgttgg caagaacaga tttgagaact 900 atagatgcat ctggtagaac aaccttaata tgtgctggtg accaaattga agaagttatg 960 gctttgactg gtatgttaag aacttgtggt tctgaagtta ttataggttt cagatccgct 1020 gcagctttgg cacaattcga acaagccgtt aatgaatcta gaagattggc aggtgctgat 1080 tttacccctc caatcgcttt accattggac ccaagagatc ctgctacaat tgatgcagtt 1140 tttgattggg gtgcaggtga aaatactggt ggtatccacg cagctgttat cttgcctgca 1200 acctctcatg aaccagcccc ttgtgttata gaagttgatg acgaaagagt cttgaacttc 1260 ttggctgatg aaatcacagg tactatcgta atcgcttcta gattggcaag atactggcaa 1320 tctcaaagat tgacaccagg tgccagagca agaggtccaa gagtaatttt cttgtcaaac 1380 ggtgctgatc aaaatggtaa tgtatatggt agaatacaat cagctgcaat aggtcaattg 1440 atcagagttt ggagacacga agctgaattg gattatcaaa gagcttctgc tgcaggtgac 1500 catgtattgc ctccagtttg ggctaatcaa atcgtaagat tcgcaaatag atctttagaa 1560 ggtttggaat ttgcctgtgc ttggaccgct caattgttac attctcaaag acacatcaat 1620 gaaatcacct tgaatatccc tgctaacatc taa 1653 <210> 58 <211> 1653 <212> DNA <213> HPDHcag <400> 58 atgtccgtca caggtagatt ggcaggtaaa atagccttga taacaggtgg tgctggtaac 60 ataggttcag aaatgactag aagattctta gccgaaggtg caactgttat tatctcaggt 120 agaaactcag ctaaattggc cgctttagca gaaagattga gatccgaagc aggtgttcct 180 gctaagagaa tagatttgga agttatggac ggttcagatc cagctgctgt tagagcaggt 240 gttgcagcta tcattggtag acatggtcac atagatatct tagttaataa tgcaggttcc 300 accggtgcac aaagaagatt ggctgaaatc ccattaaatg aaaccgatag agacttagac 360 gatgaagaag ctttgtctac aagtgttgca aatttgttgg gtatggcttg gcacttaatg 420 agaatcttaa gtcctcatat gccaccaggt tctgctatta taaacatctc caccatcttc 480 agtagagctg aatactatgg tagaatccct tacgtcgttc caaaggcagc cttgaataca 540 ttgactcaaa tcgctgcaag agaattgggt atcagaggta tcagagttaa cacaatcttt 600 ccaggtccaa ttgaatccga aagaatccaa acagtttttc aaagaatgga ccaattgaaa 660 ggtagaccag aaggtgacac agcatcacaa ttcttggcaa ctatgagatt atacagagct 720 aacgatcaag gtcaattgga aagaagattc ccaacaatct gtgacgttgc tgatgctgca 780 gttttcttgg cctctgatga agcagccgct ttgacaggtg aaactatcga agttacacat 840 ggtatggaat tgccaacatc ttcagaaacc tctttattgg ctagaacaga cttaagaact 900 attgacgcaa atggtagaac cactttgata tgtgccggtg accaaatcga agaagttatg 960 gctttgactg gtatgttgag aacttgtggt tctgaagtca ttatcggttt tagaagtgaa 1020 gctgcattgg cacaattcga acaagccatc ggtgaatcta gaagattggc tggtgaatcc 1080 tttatcccac ctattgcatt accaattgac ttgagaaacc caagtacaat agacgcatta 1140 tttgattggg ctggtgaaaa tactggtggt attcatgcag ccgtaatctt gccagcatca 1200 ggtagagaac ctgcaacaca agttatagac atcgacgatg ctcatgtcca agccttctta 1260 aacgatgaaa tcgtaggttc catcattatc gcatcaagat tggctagata ctggcaagca 1320 caaagaatcg cacctggtgc cagagcaaga gaacctagag tcatcttctt gtctaatggt 1380 gcatcaactg ctggtaatcc ttacggtaga atccaatccg cagctataga acaattaata 1440 agagtttgga gacatgaagc agccttggat tacgaaagag caaccgctgc aggtgaaaga 1500 gttttgcctg cagtttgggc atcacaaatc gttagatttg caaacagatc cttagaaggt 1560 ttggaatttg cctgtgcatg gaccgcacaa ttattacatt cccaaagaag aattaatgaa 1620 ataacattaa ctatccctgc cgacatctct taa 1653 <210> 59 <211> 1653 <212> DNA <213> HPDHct <400> 59 atgagaagaa caaaaccaga atgcttgaga agaaatgaaa gtacaatgag attgaacggt 60 aaagtcgcct taataacagg tgccgcaggt ggtatcggtt cagtagtcac aagaatgtac 120 ttgagagaag gtgctagagt tgcaatttcc tcaagatcct tggaaagagc agaacaattc 180 agagcagcct taatggccga aggtttttca ggtgacgata ttttgccagt agcatttgcc 240 agtacagact tagccggtat gcaaacagct atcgaagcaa ttgcagctag atggggtggt 300 ttggatgttt taattaacaa tgctggttca gctggtgcaa agcaaccttt gttcagaatc 360 cctttcacct cagaagattt ggctcaattg gcagctgaag gtttcacaga aaccgaaaca 420 atgagagaaa gtgctgccaa cttattgggt atgccttggc acttaacaag attggcttta 480 ccttacttga gagttggtgc ttctattatc aatgtatcta caatcttctc aagaaccaat 540 tattatggta gaataccata cgtagtccca aaatctgcct tgaacgcttt atccttgggt 600 ttggccaaac aattgggtac cactgacaga gctgtcagag ttaataccgt ttttccaggt 660 ccaatagatt cagacagaat cagaaccgtt ttcggtgcta tggacagatt gaaaggtgtc 720 ccagaaggta ctaccgctca agaatttttc gacattatga ttttggctag agcaagaggt 780 ggtgaagcac ctacaaagtg ttatccaact caagctgatg ttgctcacgt aattttattt 840 ttgggttcag atgaatctgc agccatatct ggtcataact ttgaaataac tcatggtatg 900 caagttaagg cacaatccag aacaaaattg actgcatggc cagaccaaag attgatagat 960 ttgtcaagaa gagttgtctt gattgttggt ggtgaacaag tacatgatgc tgtcacaata 1020 gcagcctccc aacaatcata tggtgcttca gttatcttga cattcagaca accagaagca 1080 gttaatactg caaaagctca attggaatcc agaggtttga accattctat agcagttacc 1140 tggttagacc cattgagaag agaatctgtt acaagtgttt tcgacttaat cagagaaaga 1200 taccacagat tggataccgt tatagttttt ccagctcatg ccgctggtca ttatggtcct 1260 gaattgatcc cagatgaacc tgaaagaatc agacaattca ccgatgaagc tattttaggt 1320 acaaccgcat tcgcagctca attgtccaga tttttattgg ctttcgatgc agaaaacgaa 1380 ttgcaagaac ctgtcaacgt tttgtttgtc acaaacgaac acgacggtgc tacaaatgca 1440 tttgaagata tctatagagc cgcagtcgaa caattgttga gagtttggag acacgaagat 1500 gaattgcaag ttgcagctgg tacaagaaga ttcgccgtaa gaaccaacca aatagtaaga 1560 tatgctaatg cagaaccaga caacttgaag tttacagccg attggttggc aacattagcc 1620 aacagagtca gaatctttga tgaattaaac taa 1653 <210> 60 <211> 747 <212> DNA <213> HPDHec <400> 60 atgatcgtat tagttactgg tgccacagcc ggtttcggtg aatgtattac aagaagattc 60 atccaacaag gtcacaaggt tatcgccacc ggtaggagac aagaaagatt gcaagaatta 120 aaggatgaat tgggtgacaa tttgtatata gctcaattgg atgttagaaa cagagctgca 180 atcgaagaaa tgttggcctc tttaccagct gaatggtgta acattgatat tttagttaac 240 aacgccggtt tggctttggg tatggaacct gcacataaag cctcagttga agattgggaa 300 actatgattg acacaaataa caagggtttg gtttacatga caagagcagt cttaccaggt 360 atggtagaaa gaaatcatgg tcacattata aacataggtt ctacagcagg ttcatggcct 420 tatgccggtg gtaatgtata cggtgctacc aaagcatttg ttagacaatt ctccttgaac 480 ttaagaaccg atttgcacgg tactgccgtt agagtcacag acattgaacc aggtttagtc 540 ggtggtactg aattttcaaa cgttagattc aagggtgacg atggtaaagc tgaaaagacc 600 tatcaaaaca ctgttgcatt gacacctgaa gatgtctccg aagccgtatg gtgggttagt 660 acattgccag ctcatgttaa catcaacacc ttagaaatga tgcctgttac tcaatcttac 720 gcaggtttaa atgtccacag acaatga 747 <210> 61 <211> 591 <212> DNA <213> HPDHed <400> 61 atgtcagaag caatcactcc tggtgcctta gaaactttat tcactggtgc aagaactcac 60 aacggttggc aaaatatgcc tgtatccgat gaaactttga gagaaatcta taatttgatg 120 aaatggggtc caacatctgc aaactgttca cctgccagaa ttgttttcgt cagatctgcc 180 gaaggtaaag aaaagttaag accagcatta tcttcaggca atttggaaaa gactttaact 240 gctcctgtaa ctgcaatagt tgcctgggat ggtgaatttt acgaaagatt gccagaatta 300 ttccctcatg gtgacgctaa gtcctggttt acagcaagtc cagccttggc tgaagaaacc 360 gctttcagaa actccagttt gcaagctgca tacttaattt ttgcttgtag agcattgggt 420 ttagataccg gtcctatgtc tggtttcgat agacaaaagg ttgatgaagc atttttcaca 480 ggtactacat tgaagtcaaa tttgttaatt aacataggtt atggtgaccc aggtaaattg 540 tacggtagat tgcctagatt agactttgat gacgcctgcg gtttagcttg a 591 <210> 62 <211> 1653 <212> DNA <213> HPDHgb <400> 62 atgaacgata tgtccgatat tccacaagac ttattaacac aaagtatagc aggtagaaga 60 ttagataaca aatgtattat tttaactggt gcagctggtt caatcggttc ctttattaca 120 agacaattat tgagagaagg tgcaagagtt atgttaacag gtagagatca atcaaaattg 180 gacgacttta ttggtgactt ggtatcagaa ggtttcgaag aaactttcat ggtttccgca 240 actggtgact gtgccgatcc tgaagtctgt agagaaatag ttgccaagac agttgccgca 300 ttcggtccta ttgatgtttt agtcaacaat gccggtgccg caggtccaaa attcacattg 360 agagatatcc ctttcaccga agcagaacaa agagcagctg actcagatca aacaatgttt 420 gactcagcca tgaatttgtt gggtgctcct tggaatatgg ccagagccgc tgcaccacat 480 atgtcagctg gtgcctctat aatcaacgtt agtactatct tttctagaac tcattactat 540 ggtagaatac cttacgtcgt tccaaagtct ggtttgaacg cattgggtaa aggtttggca 600 ttagaattgg gtgaaaaaca aggtatcaga gtaaacactt tatttccagg tcctattgaa 660 tccgaaagaa ttgaatcagt tttcgccaga atggatgaat tgcaaggtgt tgaacctggt 720 tcaactggta aagagtttag agatttgatg ataactacaa gaacctctga tggtggtttg 780 gaatataaat acccaactcc aacagatgtt gcatcaggta tcgtttggtt ggcatctgat 840 gaatcctcag ccttatctgg tcatcacatt gaagtcacca atggtatgca agtaccagca 900 caatctagat cccaattggt ttcatggcca gataagagat tggaagattt gtcaggtcaa 960 gtcgttttga ttttaggtgg tgacgactat caagaagcag ttactttcgc cgaaagacaa 1020 atccaatccg gtgcaagagt aatgttagct ttcagaaact tgaataccgt tggtcatgct 1080 agatcattag ttcaatcaag aaatttgggt gacgttcaat tatcccactt ggaccctttg 1140 agaagagaat cagttgacag atccatgcaa ttaatcgcag atcactttgg tagattagat 1200 ggtgtaatct tgttaccaca aaagccaaac ggtgaatacg gttattcctt gactacagct 1260 aatgacgaag atgttgccaa ttttgttaga gatgaagttg tcgcccctgt tgcatttgca 1320 tccgctttcg caagaaacat ggatcaattg tttgccgaag gtgaaccacc agccatcgtt 1380 tacgttacta accctagtga tagacatggt aacttgatga atgaaattat cagagcaagt 1440 gttgaagcct taatcagagg ttggagacat gaagatgaaa ctttggcaaa cactggtgaa 1500 ttgccttggg cagtacaacc aaaccaaatg gttagatatg ataccgaaga tagagaagct 1560 ttaactttcg cagccgactg ggcagccact ttaaccaaca gagttagaaa gatggatagt 1620 atcaacttat ggttaccaag aagtattaaa taa 1653 <210> 63 <211> 759 <212> DNA <213> HPDHhw <400> 63 atgatagtat tagtaacagg tgcctcctca ggtttcggta gagctattgc caagaagttc 60 ggtgaaaatg gtcataaagt aatcgcattg gcaagaagaa aggaaagatt gcaatcttta 120 caaaaggaaa tcccatactg tgaaatcttg ccttgtgata tctgcgacaa aaaggctgtc 180 aaacaagcat tggaagcctt accacaagat ttcaagtcta tcgacatttt ggttaacaac 240 gctggtttgg cattaggtat tgaacctgcc caaatgtgcg atttcaaaga ctgggaaaga 300 atgatatcag ttaatgtcac tgctttgtcc tatttgacac atttggtttt accacaaatg 360 gtcgaaagaa aaagtggtca cattataaca ttgggttcta ttgcaggtaa atatccatac 420 cctggtggta atgtatacgg tgccaccaaa gcttttgtta agcaattcgc cttgggttta 480 agagcagatt tggccggtac taacgttaga gtttcagaca ttcaaccagg tttggctgaa 540 agtgaatttt ctttagttag attcaaaggt gacaaaaaga gagctaatgc attgtatgaa 600 ggttccaacg ctttacaacc tcaagatatt gccgaagctg tctactgggt agcaacattg 660 ccaaagcata tcaacatcaa caccttagaa atgatgccta cttctcaatc attttccaca 720 ttggcagttc acaaagaata ctcattctta accaagtga 759 <210> 64 <211> 804 <212> DNA <213> HPDHka <400> 64 atgtctcaag gtagaagagc agccgataga ttagccggta aagtcgtatt gataactggt 60 gcctccgcag gtattggtga agccactgca tgggaatatt tgtttgcatc caatggtcaa 120 gccaaattga tattgacagc cagaagattg gaaaagttgg ctgctattaa agaagaaatg 180 aaaaaggaat acccaaacgt taagatacat gtcgctaagt tggatgtttc taacacagaa 240 gaaatcaaat cattgttaca atgtttacct accgagttta aatctattga tatattggtc 300 aacaacgccg gtaaagcttt aggttcagaa aaagtaggtg acattgctat tgaagatata 360 aagggtatga tggatactaa cgttataggt ttgattaatg taacacaagc agttttgcca 420 atcttgaagg aaaagaactc tggtgacatc gtcaacattg gttcagtagc cggtagagat 480 gcttatccta ccggttctat atattgcgct agtaaatttg cagttagagc cttcaccgaa 540 tctttgagaa aggaattgtt ggatactggt attagagtta tattgatcgc accaggttta 600 gtagaaactg aattttcctt agttagatac aagggtgacg aaaagagagc caatagtgtt 660 tacgaaggta tcactgcttt ggaagcagaa gatgttgctg aattaatcgt tttctctaca 720 tcaagaaaac caaacactgt tatagctgat acattgatct ttcctacctg tcaaggttcc 780 gcattccatg tccacagaag ttga 804 <210> 65 <211> 945 <212> DNA <213> HPDHms <400> 65 atgacagaaa aagtatcagt agtaggtgct ggtgttattg gtgttggttg ggccacatta 60 ttcgcctcta aaggttattc agtatctttg tacacagaaa agaaagaaac cttggataag 120 ggtatagaaa agttgagaaa ctacgtacaa gttatgaaga acaactccca aatcactgaa 180 gatgtcaaca cagtaatctc cagagttagt ccaactacaa atttggacga agctgttaga 240 ggtgcaaact ttgttattga agccgtcata gaagattacg acgctaaaaa gaaaattttc 300 ggttacttgg attctgtctt ggacaaggaa gttatattgg cctcttcaac ctctggtttg 360 ttaatcactg aagttcaaaa ggcaatgtca aagcatccag aaagagcagt tattgcccat 420 ccttggaatc cacctcactt gttaccattg gttgaaatag tccctggtga aaagacttct 480 atggaagttg tcgaaagaac caagtcattg atggaaaagt tggatagaat cgtagttgtc 540 ttaaagaaag aaatccctgg ttttattggt aacagattgg cctttgcttt attcagagaa 600 gctgtatatt tggttgacga aggtgtcgct actgtagaag atattgacaa agttatgaca 660 gctgcaatag gtttaagatg ggcttttatg ggtccattct tgacctacca tttgggtggt 720 ggtgaaggtg gtttggaata tttctttaat agaggttttg gttacggtgc aaacgaatgg 780 atgcacacat tagccaagta tgataaattc ccttacaccg gtgttactaa ggctatccaa 840 caaatgaagg aatactcctt tatcaagggt aaaactttcc aagaaatcag taagtggaga 900 gatgaaaagt tattgaaggt atacaagtta gtttgggaaa agtaa 945 <210> 66 <211> 1653 <212> DNA <213> HPDHot <400> 66 atgtttatga caagattgaa tgacaagatt gctttgatta ctggtggtgc tggtactata 60 ggtgaagtta ttacaagaag atatttggaa gaaggtgcaa cagtagttat ggctggtaga 120 aatagagaca aattagacag atatagagaa agattgataa ctgaatttca tgctttacca 180 gaaagagtta tggtagttag aatggacggt tcatccaacg cagaagttag aatgggtatt 240 gccgctgttg tagcccactt tggtagaatt gatatattgg taaataacgc aggttcagct 300 ggtgccagac aaagattgcc agctatccca ttattgagat cagaattgca agcagacgaa 360 accgaaactt tggcagattc aatcggtaac ttaataggta tcacatggaa tttgattaga 420 gctgcagctc cattcatgcc tgcaggttcc tcagttatca atattagtac aatctttgct 480 agaactgact actatggtag aatcccttat gttgtaccaa aggccgcatt acacgcctta 540 acattggcag ccgcaactga attaggtgaa agaggtataa gagttaacca aatcaaccca 600 ggtcctatcg actcagatag aattagaact gtttttagaa gaatggatga attgaagggt 660 gttccagaac aatcaactgc tgatggtttc tttcaaatga tgagattgag aaggcctaat 720 gccgaaggtg acttagttaa gggttttcct aagactttgg atgttgccaa cgttgctgta 780 tttttaggtt ccgcagaatc agctgcattg tccggtgaaa cattggatgt tacacacggt 840 atggcagttc caacagaatc aagaacaact ttgacaagta gacctggttt gagagcagta 900 gacggtagtg gtcacacaac attaatttgt gttggtgacc aaatcgaaga agcagcagcc 960 ttaactggtg tattgagagc atgtggtgcc gaagtagtta tcggttttag atctagagcc 1020 gcaatcgcta gattcgacca cttgatcgaa agaggtagac acttaccatc tcaagaacat 1080 gttgcccctg ttttgttata tttgaatcct acagaaccag aatcaataga ccaagccttg 1140 agatggatgg ccactaactt agacttgcct acaagtgtaa tcattttgcc tgcacaaaga 1200 caaccattac ctcctagtgt tgttagagcc tcagatgaag aagttgcata ctttttaaga 1260 gatgaattga gtggtatgat cgtcttggca agtagattag caagattctg gcaacaagca 1320 acattggcac ctggtaatgc acctattcaa ccaagagttt tattcatgac aaacccagac 1380 gatggtcaag gtaacttata tgcagaaata ttgagagcag gtgttgaaca attgtgtaga 1440 gtttggagac acgaatccca attggattat actagattgg cccaaatgga tgcacatcca 1500 cctcatatta gaccagtttg ggcaaaccaa ttggttagat ttgccaacaa tgaacaagaa 1560 aatttggaat actgctgtgc ttgggttgcc aagatattgt tatccgaaag aacaattgaa 1620 gaattaaact tatacttgcc tagacaaata taa 1653 <210> 67 <211> 816 <212> DNA <213> HPDHps <400> 67 atgtcctatg gtagtaaggc cgcagaaaga ttggctaaca aggtcatttt aataacaggt 60 gcttccgccg ccgctggtat cggtgccgca accgccagag aatttgctga agctgcacat 120 ggtaacataa agttgatctt gacagcaaga agaaaggata agttggacgc tttagcaaaa 180 gaattgaccg ataagtatga caacattaaa gtccacaccg tagccttgga tgttactaag 240 ttagaatcta ttggtcaatt cgtcaaggat ttgcctcaag aatttgcaga tatagacgtt 300 ttagtcaaca acgctggttt ggcattaggt agagatccaa tcggtgaaat tttgcctgaa 360 gatatacaag gcatgttcca aacaaacgta ttgggtttaa tcactttgac acaagcagtt 420 ttgccaatct tcaaagctaa aaattctggt gacatcgtta acttaggttc aattgctggt 480 agagatccat atcctggtgg tggtatctat tgtccaacta aagccgctgt aaagtctttc 540 tcacatgttt tgagaaagga attgattaat acaagaatca gagttttgga agtcgatcct 600 ggtaacgttg aaaccgaatt ttccatcact agattcaaaa acgatgttga taaggccaag 660 gctgtatatg ctaacactga accattagtc gccgaagata ttgctgaagt aatagttttt 720 ggtatcacaa gaaagcaaaa caccgttatt gcagaaactt tgatattctc aacaaaccaa 780 gcatccgcca gtcacttata caaaggtcaa gtttga 816 <210> 68 <211> 750 <212> DNA <213> HPDHra <400> 68 atgataatat tcgtcacagg tgccacagca ggttttggtg cagaaatcgc aagaaagttc 60 gtaaaggaag gtcacaaggt aatcgcaaca ggtagaagaa aggaaagatt agatgacttg 120 aaggctgaat taggtgacgc tttgtatgcc gtcgaattag acgtaaccaa tagagcatct 180 atagaagaag ctattgcttc tttgccagct gattggcaaa acattgacgt tttggtcaat 240 aacgctggtt tggcattagg tttggaaaaa gccgataagg cttccttgga agattgggac 300 aagatgatag atactaacac aaagggttta gtttacatga ccagagcagt attgccaggt 360 atggttgaaa gaaataccgg tcatgttatt aacataggtt ctactgccgg taattggcct 420 tatgctggtg gtaacgtcta cggtgcaact aaggccttcg taagacaatt ctcattgaat 480 ttgagaaccg atttgcatgg tactcacgtt agagttacaa atattgaacc aggtttgtgt 540 ggtggtacag aattttccaa cgttagattc aagggtgacg atgaaaaagt cagtaagacc 600 tacgataaca ctaacccttt aacagctgca gacgttgctg aatccgtctt ttgggctagt 660 acattgccag cacatgttaa catcaactct atcgaaatga tgcctgtttc tcaatcatta 720 gcaggtttgc aagttcacag agaaggttga 750 <210> 69 <211> 1653 <212> DNA <213> HPDHrc <400> 69 atgtctacag tcagaagatt ggaaggtaaa gttgcattga taacaggtgg tgccggtaat 60 ataggtgaag taataacaag aagatttttg gcagaaggtg ctaccgttgt tatcacaggt 120 agaaatgccg aaaaattggc tgtctacaga agaagattaa ttgacgaaga aagagtagca 180 ccagaaagag tcgttgcttt gagaatggat ggttctgaca ttgcccaagt tagagctggt 240 gttgctcaaa ttgttcacgg tggtacagat gtcccaatcc cattgcatag aatagatatc 300 ttggtcaaca atgcaggttc tgcaggtcca agaagaagat tagttgatat ccctttggaa 360 ccatccgaag ttcaacctcc agattcagaa actttggcac aagctgttgg taatttggtt 420 ggtataacat ggaatttgac aagagcagcc gcaccacaca tgccatccgg ttcatctgtt 480 attaacatct ctacaatctt ctcaagaacc gattactacg gtagaatagc ctatgtagca 540 cctaaggctg ctttgaacgc attatctgac ggtttggcca gagaattggg tgttagaggt 600 atcagagtca ataccatata tcctggtcca atcgaatcag aaagaatcta cactatgttt 660 caagctatgg acgctttaaa gggtcaacca gaaggtgaca cagctagtgg tttcttaaga 720 atgatgagat tgtctagaat cgaccaaaac ggtgaagttg ttaagagatt cccaagtcct 780 gtagatgttg caaatactgc tgtattcttg gcatctgacg aaagtgcagc ttttacaggt 840 catgcctttg aagtcacaca cggtatggaa gttccaaccg aaagtagaac cactttcgtt 900 tccagaccag gtttaagatc cgttgatgca accggtaaag ttattttaat atgtgccggt 960 gaccaagttg atgacgctgt cgcattggct gatacattga gatcatgtag agctaccgtt 1020 gtcatcggtt tcagagatcc aagagcatta gaaaaggcat ccgttttgtt gagagaacct 1080 agacacgctt tggcagctga catgtacggt agacctacaa tgactgcaga agctagattg 1140 gtcagattag atcctttaga cccaagagca gctgcccaaa ctttggaaca aatacatgcc 1200 gaattgggtg ccatccacca tgctgtcgta ttgcctggtc aatctagaca tgcaccttca 1260 gcttccttga tcgaagttga tgaccaagtt gttgaaagat tcttacacca agaattagtt 1320 ggtactattg ccttagcaag agaattggcc agattttggg aagaatatcc atccggttct 1380 tcaatgcata gagttttatt tgtttccaat cctgacgatc aacaaggtaa ccaatacagt 1440 cacatcttga gagctgcagt tgaacaatta gtcagagtat ggagacacga atccgaatat 1500 gactccgtta accctgcaca ccaacaagaa ggtcaatcct cagcagctgt ttgggctaac 1560 caattaataa gatatgttaa taacgaaatg gctaacttgg acttcacttg cgcctgggtt 1620 gccaagttat taggttccga tagaagaatc taa 1653 <210> 70 <211> 969 <212> DNA <213> HPDHsi <400> 70 atgtttatcc acatgaagag tattaacaaa gtagccgtag taggtgctgg tattatcggt 60 gtaggttgga caactttatt attgactaaa ggttacaagg ttaatttgta caccgaaaag 120 aaagaaactt tagaaaaggg tttggcaaag gtatctgcct atttggttaa tttgaaaaat 180 ttgagaatga tagatggtga accaatcgac taccaaagaa atttgacagg tactacaaag 240 attgatgaag ccattcaaaa cgttgaattt gtcatcgaag ctatcatcga agattactcc 300 gctaagaaaa atttgtttaa ttacttggat agtcaattgc ctagagacat cattatagca 360 tcttcaacat ctggtttgtt aatgaccgaa atccaaaagg ctatgaccag atatccagat 420 agaggtgtta tcgcacatcc ttggaatcca cctcacttgt taccattggt agaaattgtt 480 ccaggtgaca aaacctcaaa gtacactttg gatgttacaa aggacttcat ggaaaagttg 540 gatagagttg tcgtagtttt gaagaaagaa gttccaggtt tcattggtaa tagattggcc 600 tttgctttat tcagagaagc agtcaacttg atagatgaag gtgtcgccac tgtagaagat 660 attgacaaag ttatgacagc tgcaataggt ttaagatggg cttttatggg tccattcttg 720 acatatcatt tgggtggtgg tgaaggtggt attgaatact ttttctccaa aggttttggt 780 tatggtgcca atgaatggat gtacaccttg gctaaatggg ataagttccc ttatactgct 840 gttaaaaaga tcgttgatca aatgaaggaa tacgagttta ttaagggtaa aaatttccaa 900 gatttgtcaa gatggagaga tgaaactttg gttagagtat acaaggcagt ttttggtgaa 960 aagagatga 969 <210> 71 <211> 1653 <212> DNA <213> HPDHsl <400> 71 atgaatgcaa tgggtaaaac agaaacacaa ggtagattag ctggtaaagt agccttgata 60 actggtgccg ccggtaactt aggtggttct atcgtaagaa gatatttggc cgaaggtgca 120 acagttgtca tgtccggtag agatgctgcc agaactcaag cagctttaga tgcagcagta 180 gctgacgttg gtggtactgc ttcaattgtc actatggatg gtgcaaatat cgaatccgtt 240 agagcaggta tcgcagatgt tgtcgcaaga catggtagaa tagacgtctt ggttaataac 300 gcaggttcag ctggtccaaa gcaagttttg ggtgacttac ctttgttgcc agaagaattg 360 gaaggtacac ctgattcaga aacagttggt gacgcagcaa gaaatataat ggttgtcgca 420 tggaacttag ttagagctgc cgcatctgct atgggtgaag gtggttccat cattaatgtt 480 tccacaatat tctcaagaac tgattatttt ggtagagcag cttatacagt tccaaaagct 540 gcattgaata tcttatccaa aggtatcgca agagaattgg gtaccagagg tatcagagtc 600 aatcaattgt atcctggtcc aatcgcctca gaaagaatca gaacagtttt cgccgcaatg 660 gataagttga aaggtgacga tgccggttca actgccaacc atttctttaa tttgatggct 720 ttggaaagaa gtgtaggtga agatgccaaa gcaaagacct tccctacacc agctgatatc 780 gcaaacactt gcgttttctt gggttcagac gaatccgctg cctttgcagc acacgacttt 840 gaagttacac atggtatggc agtacctttg gaatcaaaat ccgaattttt gcatagacct 900 gacttgagag caatagatgg tgacggtcat ggtatcttga tttcagccgg taaccaatta 960 gacgatgcat tggctatggc aggtttgttc gcagcacaag gttcaaaagt cttgttgggt 1020 tttcacgcac aagctgcatt ggacgatgca gcagaaagat tgccagccgg tgtcaaagca 1080 gttagatttc caagacatga ccatatggca atggacgctg aattggccag attcacagca 1140 catgtagccg caattgacgg tgctattata ttgccaacaa gaggtccagg ttactttcat 1200 ggtgcattga acgaagcttc cgacgctgcc gttgaaagat ttgtcgatga cgaattggaa 1260 ggtgcaattg ctgttgcaag aacattagat agatattggc aacaaggtca agacttgcct 1320 ggtgacccta gagtcttgtt tatcacaaac cctgatgatg gtgcaggtaa tggttgggca 1380 gatgttggta gagcagcaat cgaagaattg ttgagagttt ggagagatga agctagagta 1440 gccgcaacac aaggtaaaac tcctagacct gtttggggta accaaattgt tagatttggt 1500 aataacgaac cagaaaatag agactttgct gcaggtcatg ccgcaagatt gttattaggt 1560 ggtagacaaa tagaaccaat caatttgtac atccctgcat ctatcacaaa agccactggt 1620 gccagaagag ccttagcagg taccgcagaa taa 1653 <210> 72 <211> 867 <212> DNA <213> HPDHsm <400> 72 atggctatct taatcactgg tgcctcatca ggtttcggtg ctgctatggc tactgccttc 60 gtccaagctg gttacttagt catcggtaca gctagaagaa ccgaaaaatt agcagatttg 120 catgccaagt taggtgacag atttcaacct ataactatgg atatgacatc aaaggatgac 180 attcaagctg cattagcaca catacaaacc ttgccagaat acttcagaga aatagattgt 240 ttgatcaata acgctggttt ggcattaggt ttggatagag ccgaccaagc tgatttcacc 300 gactgggaaa ctatgatcca aacaaacatc atcggtttaa cctatttgac tagacaaata 360 ttgcctaaga tggttgaaaa taagagaggt tacatcatta acatgggttc aattgctggt 420 aattatccat acgcaggttc caacgtctat ggtgcaacta aggcctacgt tagacaattc 480 tctttgaatt tgagagctga tttgcatggt accggtgtaa gagttactaa tatagaacca 540 ggtttggttg aaggttctga attttcaaac gtcagattca aaggtgacga taagagagcc 600 gctcaattat atgaatacgc aaacgccttg acaagtgaag atatagctca aaccgcatta 660 tggttgtatc aaagacctgc ccacatgaat gttaacacta tcgaattaat gccaactaca 720 caatcttggg ctggtatgca agtttacaaa gatacaccac ctccacctcc acctgttgtc 780 gaaccaaagc ctgccgactt tgctccacaa cctgctccac aagaagaatc cggtttcttt 840 tctaagatca catctttgtt caagtga 867 <210> 73 <211> 957 <212> DNA <213> HPDHst <400> 73 atgagacaaa tcaagacagt cagtgttatt ggtgctggtg ttataggtgc tggttggtcc 60 acattattgg cattgaaggg ttacgaaaat tggttttaca ctgaaaagaa agaaacattg 120 gataaaggtt tggctaaaat caagggttac ttaaatgttt tgtatgaata caaattaatt 180 gacaaagaac cagattatta catgcaaaga atacatccaa ccacaaaatt ggacgaagct 240 atttccaata ctgacttcgt cttggaagca attgttgaag aatacggtgc taagaaagcc 300 ttgttcaaac aattggacga aaaattagat aaagacgtta tcttagcatc ttcaacttcc 360 ggtttattga tgactgaaat ccaaaaggca atgacaaagt acccagaaag aggtattata 420 gctcatcctt ggaatccacc tcatttgttg cctttggtcg aaatcgttcc tggtgaaaag 480 acatcacaag acaccatcta cgcaacaaag gattttatgg aaaacaagtt ggaaagagtc 540 gtcgtagttt tgaaaaagga aatcagtggt ttcataggta atagattggc ctttgcattg 600 ttcagagaag cagtttactt ggttgatgaa ggtattgcca ctgttgaaga tattgataaa 660 gttatgacag ctgcaatcgg tttaagatgg gccttcatgg gtccattttt gacttatcac 720 ttaggtggtg gtgaaggtgg tttggaatat ttcttttcca gaggtttcgg ttatggtgct 780 aacgaatgga tgcatacttt agcaaagtat gataagttcc catatactgg tgttgtaaag 840 tcagttcaac aaatgaaaga atatgaattt gtcaaatcca agacattcca agaattgagt 900 agatggagag atgaaaagtt gataagtttg ataaagttgt tgagaggtaa aatataa 957 <210> 74 <211> 480 <212> PRT <213> AADHab <400> 74 Met Glu Ile Asn Glu Lys Asp Leu Lys Leu Ile Ile Glu Asn Val Leu 1 5 10 15 Asp Lys Leu Glu Ser Asp Lys Ser Asp Lys Lys Ile Phe Asp Ser Ser 20 25 30 Leu Asn Ile Pro Ser Ser Gly Gln Asp Gly Ile Phe Glu Glu Val Asn 35 40 45 Ala Ala Val Asp Ala Ala Glu Ala Ala His Leu Glu Leu Met Lys Leu 50 55 60 Thr Leu Glu Lys Arg Arg Glu Ile Ile Arg Ser Ile Arg Lys Ile Ile 65 70 75 80 Met Ser Asn Leu Glu Glu Ile Ser Lys Leu Ala Val Glu Glu Thr Thr 85 90 95 Phe Gly Arg Val Glu Asp Lys Ile Glu Lys Asn Arg Leu Ala Ala Leu 100 105 110 Lys Thr Pro Gly Ile Glu Asp Leu Glu Pro Thr Ala Tyr Ser Asp Asp 115 120 125 Asn Gly Met Thr Leu Met Glu Arg Ala Ala Tyr Gly Val Ile Gly Ser 130 135 140 Ile Ile Pro Ser Thr Asn Pro Thr Ser Ser Val Ile Asn Asn Gly Ile 145 150 155 160 Ser Met Ile Ala Gly Gly Asn Ser Val Val Phe Asn Pro His Pro Ala 165 170 175 Ala Lys Arg Ser Ser Cys Phe Thr Ile Ser Leu Ile Asn Gln Ala Ile 180 185 190 Val Lys Ala Gly Gly Pro Pro Asn Val Leu Cys Ala Ile Ala Asn Pro 195 200 205 Thr Ile Asp Ser Ala Gln Thr Leu Met Lys His Pro Lys Ile Lys Leu 210 215 220 Leu Val Val Thr Gly Gly Pro Ala Val Val Lys Thr Ala Met Asn Ser 225 230 235 240 Asp Lys Lys Val Ile Ala Ala Gly Pro Gly Asn Pro Pro Cys Val Val 245 250 255 Asp Glu Thr Ala Asp Leu Val Lys Ala Gly Arg Asp Ile Val Asn Gly 260 265 270 Ala Gly Phe Asp Asn Asn Ile Val Cys Ile Cys Glu Lys Glu Ile Leu 275 280 285 Ala Val Ser Gln Ile Ala Asp Gln Leu Lys Glu Glu Met Ile Lys Asn 290 295 300 Gly Ala His Glu Leu Lys Gly Glu Gln Ile Glu Lys Val Thr Lys Leu 305 310 315 320 Val Ile Thr Asp Pro Gly Lys Pro Gly His Glu Gly Ala Pro Asn Lys 325 330 335 Lys Tyr Val Gly Lys Asn Ala Ser Val Ile Ala Arg Asp Ile Gly Leu 340 345 350 Asp Ile Ser Glu Gln Thr Lys Ile Leu Leu Cys Glu Val Asp Arg Tyr 355 360 365 His Pro Leu Val Trp Thr Glu Gln Leu Leu Pro Val Ile Pro Leu Val 370 375 380 Arg Phe Asn Asn Val Asp Glu Ala Ile Asp Phe Ala Val Gln Cys Glu 385 390 395 400 His Asn Phe Arg His Ser Ala Ser Ile His Ser Lys Asn Ile Ala Thr 405 410 415 Leu Ser Arg Met Ala Lys Leu Met Asn Cys Ser Leu Phe Ile Lys Asn 420 425 430 Gly Pro Asn Tyr Ser Gly Leu Gly Phe Gly Gly Ala Gly Tyr Thr Ser 435 440 445 Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Leu Thr Arg Ala Arg Thr 450 455 460 Phe Thr Arg Glu Arg Arg Cys Val Leu Val Gly Tyr Phe Arg Ile Val 465 470 475 480 <210> 75 <211> 477 <212> PRT <213> AADHal <400> 75 Met Val Asp Gln Thr Leu Ile Glu Gln Ile Thr Arg Ala Val Leu Thr 1 5 10 15 Gln Met Lys Ala Gly Lys Asp Ala Ala Val Ser Gly Asp Gly Ile Phe 20 25 30 Ala Thr Val Asp Gln Ala Val Ala Ala Ala Arg Gln Ala Tyr Gln Glu 35 40 45 Leu Arg Leu Leu Thr Leu Glu Lys Arg Glu Thr Leu Ile Arg Ala Ile 50 55 60 Arg Asp Ala Ala Phe Ala Asn Ala Ala Val Ile Ala Gln Met Ala Val 65 70 75 80 Gln Glu Ser Gly Met Gly Arg Val Glu Asp Lys Ile Leu Lys Asn Gln 85 90 95 Leu Ala Ala Arg Lys Thr Pro Gly Thr Glu Asp Leu Thr Ser Arg Ala 100 105 110 Trp Ser Gly Asp His Gly Leu Thr Leu Val Glu Met Ala Pro Tyr Gly 115 120 125 Val Ile Gly Ala Ile Thr Pro Thr Thr Asn Pro Ser Glu Thr Val Ile 130 135 140 Cys Asn Ser Ile Gly Met Ile Ala Ala Gly Asn Ser Ile Val Phe Ser 145 150 155 160 Pro His Pro Thr Ala Gln Asn Thr Ser Leu Thr Thr Ile Arg Leu Leu 165 170 175 Asn Glu Ala Ile Val Lys Ala Gly Gly Pro Asp Asn Leu Leu Thr Ala 180 185 190 Val Ala Glu Pro Ser Ile Glu Ala Ala Asn Ala Met Met Arg His Pro 195 200 205 Gly Ile Gln Met Leu Val Ala Thr Gly Gly Pro Ala Val Val Lys Ala 210 215 220 Val Leu Ser Ser Gly Lys Lys Ala Ile Gly Ala Gly Ala Gly Asn Pro 225 230 235 240 Pro Ala Val Val Asp Glu Thr Ala Asp Ile Ala Lys Ala Ala Lys Asp 245 250 255 Ile Val Ala Gly Cys Ser Phe Asp Asn Asn Leu Pro Cys Ile Ala Glu 260 265 270 Lys Glu Ile Ile Ala Val Gly Arg Ile Ala Asp Glu Leu Ile Ser Tyr 275 280 285 Leu Gln Lys Tyr Gly Ala Tyr Leu Ile Ser Gly Arg Asp Ile Glu Arg 290 295 300 Leu Met Glu Val Val Leu Thr Glu Arg Thr Glu Glu Met Ala Pro Gly 305 310 315 320 Cys Val Gly Lys Pro Arg Arg Val Tyr Gly Val Asn Lys Asp Tyr Ile 325 330 335 Gly Lys Asp Ala Lys Phe Ile Leu Ser Lys Ile Asn Ile Gln Ala Pro 340 345 350 Asp His Ile Arg Val Ile Leu Cys Glu Thr Pro Ala Asp His Pro Phe 355 360 365 Val Leu Glu Glu Leu Met Met Pro Val Leu Pro Leu Val Ser Val Arg 370 375 380 Asp Ile Asp Ala Ala Ile Asp Leu Ala Val Lys Val Glu His Gly Asn 385 390 395 400 Arg His Thr Ala Val Met His Ser Lys Asn Val Asp Tyr Met Thr Arg 405 410 415 Leu Ala Lys Ala Ile Glu Thr Thr Ile Phe Val Lys Asn Ala Pro Ser 420 425 430 Tyr Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr Phe Thr Ile 435 440 445 Ala Gly Pro Thr Gly Glu Gly Leu Thr Ser Ala Arg Ser Phe Thr Arg 450 455 460 Gln Arg Arg Cys Ala Leu Val Asp Ala Phe Ser Ile Val 465 470 475 <210> 76 <211> 464 <212> PRT <213> AADHbs <400> 76 Met Asp Glu Ile Met Ile Lys Asn Ile Val Arg Glu Val Leu Met Lys 1 5 10 15 Thr Asn Leu Asp Ile Ser Cys Lys Ser Asp Gln Gly Val Phe Glu Asp 20 25 30 Val Asn Asp Ala Ile Asp Ala Ala Tyr Ile Ala Gln Arg Asp Trp Tyr 35 40 45 Met Asn Tyr Lys Val Glu Asp Arg Arg Arg Ile Ile Glu Ala Ile Arg 50 55 60 Val Val Ala Arg Glu Asn Ala Pro Met Phe Ala Lys Met Ile His Glu 65 70 75 80 Glu Thr Gly Met Gly Arg Tyr Glu Asp Lys Ile Thr Lys Asn Leu Ala 85 90 95 Val Ile Asp Lys Thr Pro Gly Val Glu Cys Leu Val Thr Asp Ala Ile 100 105 110 Ser Gly Asp Ser Gly Leu Met Ile Glu Glu Gln Ala Pro Phe Gly Val 115 120 125 Ile Gly Ala Ile Thr Pro Ser Thr Asn Pro Thr Glu Thr Ile Ile Asn 130 135 140 Asn Thr Ile Ser Met Ile Gly Gly Gly Asn Ala Val Val Phe Asn Val 145 150 155 160 His Pro Gly Ala Lys Lys Val Cys Ala Val Cys Leu Gln Ile Leu His 165 170 175 Lys Thr Ile Val Glu Asn Gly Gly Pro Ala Asn Leu Ile Thr Met Gln 180 185 190 Arg Lys Pro Asp Met Glu Ala Val Asn Lys Leu Thr Ala Ser Pro Lys 195 200 205 Ile Arg Leu Met Val Gly Thr Gly Gly Met Gly Met Val Asn Ala Leu 210 215 220 Leu Lys Ser Gly Lys Lys Thr Ile Gly Ala Gly Ala Gly Asn Pro Pro 225 230 235 240 Val Val Val Asp Asp Thr Ala Asp Leu Asp Lys Ala Ala Ser Glu Ile 245 250 255 Tyr Arg Gly Ala Ser Phe Asp Asn Asn Leu Leu Cys Leu Ala Glu Lys 260 265 270 Glu Thr Phe Val Met Asp Asn Val Ala Asp Glu Leu Ile Arg Lys Met 275 280 285 Cys Ala Cys Gly Ala His Leu Ile Thr Pro Gln Glu Thr Glu Gln Leu 290 295 300 Leu Lys Val Ile Phe Leu Glu Lys Asp Gly Lys Tyr Ser Val Asn Lys 305 310 315 320 Lys Trp Val Gly Lys Asp Ala Ser Leu Ile Leu Glu Ser Ile Gly Ile 325 330 335 Lys Asp Ala Asp Thr Arg Leu Val Leu Cys Glu Val Pro His Asp His 340 345 350 Pro Phe Val Leu Val Glu Gln Leu Met Pro Ile Met Pro Ile Val Arg 355 360 365 Cys Lys Thr Phe Glu Asp Cys Val Lys Tyr Ala Val Val Ala Glu Asn 370 375 380 Gly Asn Arg His Thr Ala Ser Met Phe Ser Lys Asn Val Asp His Met 385 390 395 400 Thr Arg Phe Ala Lys Glu Ile Glu Thr Thr Ile Phe Thr Lys Asn Ala 405 410 415 Ala Thr Leu Val Gly Val Gly Ile Gly Gly Glu Gly His Thr Thr Met 420 425 430 Thr Ile Ala Gly Pro Thr Gly Glu Gly Leu Thr Cys Ala Arg Ser Phe 435 440 445 Thr Arg Arg Arg Arg Cys Met Leu Ala Asp Gly Gly Leu Arg Val Ile 450 455 460 <210> 77 <211> 484 <212> PRT <213> AADHbw <400> 77 Met Asp Val Arg Gln Gln Asp Val Glu Arg Ile Val Val Glu Val Leu 1 5 10 15 Lys Lys Met Met Ser Asp Gln Pro Thr Ala Ala Ala Thr Thr Val Val 20 25 30 Ala Ala Ser Gly Cys Asp Cys Gly Asp Phe Gly Leu Phe Asp Arg Leu 35 40 45 Glu Asp Ala Val Gln Ala Ala Glu Ala Ala Gln Lys Lys Ile Ser Thr 50 55 60 Val Ala Met Arg Asp Lys Ile Ile Ala Ala Ile Arg Lys Ala Gly Leu 65 70 75 80 Glu Asn Ala Lys Ala Phe Ala Glu Ile Ala His Asn Glu Thr Gly Met 85 90 95 Gly Arg Val Ser Asp Lys Ile Ala Lys Asn Ile Leu Val Cys Glu Arg 100 105 110 Thr Pro Gly Thr Glu Cys Leu Ser Pro Met Ala Ile Ser Gly Asp Met 115 120 125 Gly Leu Thr Leu Ile Glu Asn Ala Pro Trp Gly Val Ile Ala Ser Val 130 135 140 Thr Pro Ser Thr Asn Pro Thr Ala Thr Val Ile Asn Asn Ala Ile Ser 145 150 155 160 Met Ile Ala Gly Gly Asn Ser Val Ile Phe Ala Pro His Pro Asn Ala 165 170 175 Lys Arg Ala Ser Gln Thr Ala Ile Gln Val Leu Asn Lys Ala Ile Ile 180 185 190 Glu Ala Thr Gly Val Ala Asn Leu Leu Val Ala Val Lys Glu Pro Thr 195 200 205 Ile Glu Val Ala Gln Glu Leu Phe Ser His Pro Arg Ile Lys Leu Leu 210 215 220 Val Val Thr Gly Gly Glu Ala Val Val Ala Gln Ala Arg Lys Val Ala 225 230 235 240 Thr Met Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro Pro Val Val Val 245 250 255 Asp Glu Thr Ala Asn Ile Ala Arg Ala Ala Arg Ser Ile Tyr Asp Gly 260 265 270 Ala Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu Lys Glu Ile Ile 275 280 285 Ala Val Asp Ser Ile Ala Asp Gln Leu Lys Ala Glu Met Lys Ala Ile 290 295 300 Gly Ala Val Glu Ile Ser Leu Glu Gln Ala Asp Ala Val Ala Arg Val 305 310 315 320 Val Leu Arg Asn Tyr Pro Gln Val Glu Gly Gly Lys Ala Pro Asn Pro 325 330 335 Asn Pro Lys Trp Val Gly Arg Asp Ala Ala Leu Ile Ala Lys Ala Ala 340 345 350 Gly Ile Asp Val Pro Asp Ser Cys Arg Leu Leu Ile Val Asp Val Lys 355 360 365 Arg Asp Ile Asn His Val Phe Ala Arg Val Glu Gln Leu Met Pro Val 370 375 380 Ile Pro Leu Leu Arg Ala Ala Asn Val Asp Glu Ala Ile Glu Trp Ala 385 390 395 400 Leu Ile Leu Glu Arg Gly Leu Ser His Thr Ala Gly Met His Ser Arg 405 410 415 Asn Ile Asp Asn Met Asp Lys Met Ala Arg Ala Met Asn Thr Ser Leu 420 425 430 Phe Val Lys Asn Gly Pro His Leu Ala Ala Leu Gly Ala Gly Gly Glu 435 440 445 Gly Trp Thr Thr Met Thr Ile Ser Thr Pro Thr Gly Glu Gly Val Thr 450 455 460 Cys Ala Arg Ser Phe Val Arg Leu Arg Arg Cys Cys Val Val Asp Asn 465 470 475 480 Phe Arg Ile Val <210> 78 <211> 437 <212> PRT <213> AADHcs <400> 78 Met Lys Ser Ser Ile Tyr Ser Ser Val Ser Glu Ala Val Met Ala Ala 1 5 10 15 Arg Gly Ala Phe Asn Arg Tyr Ala Asp Leu Thr Leu Asn Glu Arg Lys 20 25 30 Glu Ile Ile Glu Ala Val Lys Ala Ala Val Arg Pro Arg Ile Thr Gln 35 40 45 Leu Ala Glu Met Thr Ala Ala Glu Thr Gly Met Gly Asn Val Ala Asp 50 55 60 Lys Ile Glu Lys Ile Arg Leu Ala Val Glu Lys Thr Pro Gly Val Glu 65 70 75 80 Asp Leu Ile Thr Glu Val Asn Thr Gly Asp His Gly Met Thr Leu Tyr 85 90 95 Glu Leu Ser Ala Phe Gly Val Val Cys Ala Val His Pro Cys Thr Asn 100 105 110 Pro Cys Ala Thr Leu Ile Ser Asn Thr Ile Gly Ile Leu Ala Ala Gly 115 120 125 Asn Ala Val Val His Cys Pro His Pro Arg Ala Val Arg Val Ser Gln 130 135 140 Tyr Leu Thr Gly Met Ile Ser Asp Ala Val Arg Asp Val Cys Gly Ile 145 150 155 160 Asp Asn Leu Val Val Thr Val Gly Glu Ser Leu Met Gly Ser Thr Arg 165 170 175 Glu Ile Met Ser His Pro Asp Val Asp Leu Ile Val Cys Thr Gly Gly 180 185 190 Ser Gly Pro Leu Arg Gln Ala Met Ser Ala Gly Lys Lys Val Ile Gly 195 200 205 Ala Gly Pro Ala Asn Pro Val Ala Ile Val Asp Glu Thr Ala Asp Leu 210 215 220 Glu Arg Ala Ala Arg Asp Ile Val Lys Gly Ala Ser Phe Asp Asn Asn 225 230 235 240 Leu Met Cys Thr Ser Glu Lys Ser Ile Val Ala Val Glu Lys Ile Ala 245 250 255 Asp Glu Leu Ile Ser His Leu Glu Arg Asn Gly Val Tyr Tyr Thr Arg 260 265 270 Ser Ala Glu Glu Ile Asp Arg Leu Tyr Cys Thr Ala Val Thr Glu Asp 275 280 285 Phe Val Ile Asn Lys Lys Met Glu Gly Gln Ser Ala Asp Val Ile Leu 290 295 300 Asp Ala Ala Lys Ile Pro His Arg Lys Gly Ile Lys Leu Ile Val Ala 305 310 315 320 Glu Thr Asp Arg Thr His Pro Phe Ala Thr Leu Glu Leu Leu Met Pro 325 330 335 Leu Leu Pro Leu Val Arg Ala Arg Asp Phe Asp Glu Ala Leu Glu Ala 340 345 350 Ala Leu Asp Ile Glu Gln Gly Phe Arg His Thr Ala Thr Ile His Ser 355 360 365 Glu Ser Val Glu Arg Met Asn Arg Ala Ala Lys Lys Leu Gln Thr Ala 370 375 380 Val Phe Val Lys Asn Gly Pro Ser Leu Leu Gly Ile Gly Phe Asp Lys 385 390 395 400 Glu Gly His Thr Ser Phe Thr Ile Gly Thr Ile Thr Gly Glu Gly Thr 405 410 415 Thr Ser Ala Arg His Phe Ala Arg Arg Arg Arg Cys Thr Leu Thr Ser 420 425 430 Gly Phe Ser Ile Arg 435 <210> 79 <211> 485 <212> PRT <213> AADHho <400> 79 Met Ala Leu Arg Glu Asp Arg Ile Ala Glu Ile Val Glu Arg Val Leu 1 5 10 15 Ala Arg Leu Asp Gly Asn Ser Gly Ser Ser Ala Ala Pro His Ser Gly 20 25 30 Ser Gly Ala Pro Ala Ala Thr Ala Gly Gly Ala Ser Leu Asp Ile Pro 35 40 45 Arg Gly Thr Leu Gly Val Tyr Ala Asp Ala Asp Ala Ala Val Asn Ala 50 55 60 Ala Arg Arg Gly Phe Ala Ala Asn Glu Ala Leu Pro Leu Arg Thr Arg 65 70 75 80 Gln Ala Met Ile Asp Ala Met Arg Lys Val Ala Arg Ala His Ile Pro 85 90 95 Glu Leu Ala Arg Tyr Ala Val Ala Glu Thr Gly Leu Gly Arg Tyr Glu 100 105 110 Asp Lys Leu Ala Lys Asn Glu Leu Val Ile Ala Lys Thr Pro Gly Pro 115 120 125 Glu Ile Leu Ala Pro Val Ala Tyr Thr Gly Asp Asp Gly Leu Thr Leu 130 135 140 Thr Glu Arg Ala Pro Tyr Gly Val Ile Gly Ala Ile Thr Pro Cys Thr 145 150 155 160 Asn Pro Thr Glu Thr Val Ile Cys Asn Ala Ile Gly Met Leu Ser Gly 165 170 175 Gly Asn Ala Val Val Phe Asn Val His Pro Ser Ala Ala Arg Val Cys 180 185 190 Asn Trp Leu Val His Leu Leu Asn Glu Ala Ile Met Ser Val Gly Gly 195 200 205 Pro Arg Asp Ala Ile Thr Ser Val Glu Ser Pro Thr Ile Asp Ser Ala 210 215 220 Gln Thr Leu Met Thr His Ala Gly Val Arg Leu Val Val Val Thr Gly 225 230 235 240 Gly Pro Gly Val Val Arg Ala Ala Met Lys Ser Gly Lys Lys Val Ile 245 250 255 Ala Ala Gly Pro Gly Asn Pro Pro Ala Val Val Asp Glu Thr Ala Asn 260 265 270 Leu Ala Lys Ala Ala Ala Ala Ile Ile Lys Gly Ala Ser Ile Asp Asn 275 280 285 Asn Ile Ile Cys Thr Ala Glu Lys Glu Ile Val Ala Val Ala Ser Ile 290 295 300 Ala Asp Glu Leu Ser Arg Leu Leu Gly Gln Arg Gly Ala Leu Val Leu 305 310 315 320 Gly Asp Ala Gln Val Arg Ala Leu Glu Arg Val Val Leu Asp Gly Glu 325 330 335 His Val Asn Lys Glu Trp Val Gly Lys Asp Ala Ser Arg Ile Ala Glu 340 345 350 Gln Ile Gly Leu Arg Gly His Gly Ser Asp Leu Arg Leu Leu Val Cys 355 360 365 Pro Val Asp Glu Gly His Pro Phe Val Gln His Glu Leu Leu Met Pro 370 375 380 Val Ile Gly Leu Val Arg Val Ser Asp Ala Thr Glu Ala Met Ala Thr 385 390 395 400 Ala Val Arg Val Glu His Gly Phe Cys His Thr Ala Val Met His Ser 405 410 415 Thr His Ile Asp Arg Leu Ser Ala Met Ala Arg Val Cys Asn Ala Ser 420 425 430 Ile Phe Val Lys Asn Asp Cys Asn Leu Ala Gly Leu Gly Leu Gly Gly 435 440 445 Glu Gly Phe Thr Ser Phe Thr Ile Ala Ser Pro Thr Gly Glu Gly Leu 450 455 460 Thr Thr Ala Arg Asp Phe Thr Arg Val Arg Arg Cys Thr Leu Lys Glu 465 470 475 480 Ser Phe Arg Phe Val 485 <210> 80 <211> 467 <212> PRT <213> AADHhs <400> 80 Met Lys Ile Lys Glu Asn Glu Leu Asp Lys Ile Val Asn Gln Val Ile 1 5 10 15 Ser Ser Leu Asn Asn Lys Gln Asn Ser Asn Asp Phe Asn Thr Lys Ile 20 25 30 Asn Tyr Gly Ile Phe Ser Thr Met Asp Glu Ala Ile Ala Glu Ala Val 35 40 45 Lys Ala Gln Ala Cys Leu Gln Leu Asn Tyr Ser Thr Glu Ala Arg Glu 50 55 60 Lys Ile Ile Lys Ser Ile Arg Lys Asn Val Ser Lys His Val Glu Lys 65 70 75 80 Ile Ser Glu Met Ala Val Glu Glu Thr Asp Met Gly Arg Ile Glu Asp 85 90 95 Lys Ile Ile Lys Asn Asn Leu Ala Ile Asn Lys Thr Pro Gly Thr Glu 100 105 110 Asp Leu Arg Thr Glu Ala Phe Ser Gly Lys Lys Gly Leu Thr Ile Val 115 120 125 Glu Glu Ala Pro Phe Gly Val Ile Cys Ser Ile Ala Pro Val Thr Asn 130 135 140 Pro Thr Glu Thr Ile Ile Ser Asn Ala Ile Ser Met Ile Ala Ser Cys 145 150 155 160 Asn Gly Val Val Phe Asn Ser His Pro Gly Ala Lys Lys Val Ser Lys 165 170 175 Tyr Ile Ile Glu Val Leu Asn Lys Val Ile Met Glu Ala Gly Gly Pro 180 185 190 Glu Asn Leu Leu Thr Ala Val Asn Glu Pro Thr Leu Gln Thr Val Glu 195 200 205 Ser Cys Met Arg Asp Asp Arg Ile Ala Met Ile Val Ala Thr Gly Gly 210 215 220 Pro Gly Val Val Asn Ala Ala Leu Ser Ser Gly Lys Lys Ala Ile Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Val Leu Val Asp Asp Thr Val Asp Leu 245 250 255 Lys Arg Val Ala Lys Asp Ile Ile Asn Gly Ala Ser Phe Asp Asn Asn 260 265 270 Leu Pro Cys Thr Ser Glu Lys Ala Ile Val Ala Leu Glu Ser Ile Ala 275 280 285 Asp Ser Leu Leu Asn Glu Met Thr Asn Gln Asn Ala Gln Leu Val His 290 295 300 Asp Ile Lys Ala Leu Glu Arg Val Ile Leu Asn Asp Asp Gly Ser Ile 305 310 315 320 Asn Lys Ala Leu Val Gly Lys Asp Ala Ala Phe Ile Leu Asn Lys Ala 325 330 335 Gly Leu Lys Ala Lys Ser Glu Asp Leu Arg Leu Val Ile Val Asp Val 340 345 350 Asp Leu Arg His Pro Phe Val Gln Lys Glu Gln Leu Met Pro Val Ile 355 360 365 Pro Leu Val Arg Ala Lys Asn Phe Asn Glu Ala Met Glu Met Gly Val 370 375 380 Asp Ile Glu Glu Gly Asn Arg His Thr Ala Ile Ile His Ser Lys Asn 385 390 395 400 Val Asp Asn Leu Thr Lys Phe Ala Lys Lys Ile Glu Thr Thr Ile Tyr 405 410 415 Val Lys Asn Ala Pro Ser Tyr Ala Gly Ile Gly Ala Gly Gly Glu Gly 420 425 430 Tyr Ala Thr Phe Thr Ile Ala Gly Pro Thr Gly Glu Gly Leu Thr Ser 435 440 445 Ala Arg Ser Phe Thr Arg Lys Arg Arg Cys Val Leu Val Asp Gly Phe 450 455 460 Ser Ile Ile 465 <210> 81 <211> 465 <212> PRT <213> AADHma1 <400> 81 Met Gln Thr Asp Ala Gln Gln Ile Glu Ser Ile Val Arg Arg Val Ile 1 5 10 15 Glu Gln Leu His Ser Pro Gln Arg Asp Gly Glu Ser Tyr Gly Val Phe 20 25 30 Arg Thr Leu Asp Asp Ala Val Ala Gly Ala Gln Gly Ala Tyr Lys Lys 35 40 45 Ile Arg Thr Met Ala Gln Arg Glu Ala Ile Ile Ala Ala Ile Arg Arg 50 55 60 Thr Gly Ser Glu Asn Val Gln Ala Leu Ser Glu Leu Ala Val Gln Glu 65 70 75 80 Thr Gly Phe Gly Arg Val Glu Asp Lys Ile Arg Lys His Arg Leu Val 85 90 95 Leu Asp Lys Thr Pro Gly Ile Glu Ala Ile Val Pro Met Ala Val Thr 100 105 110 Gly Asp His Gly Leu Ser Leu Ile Glu Asn Ala Pro Trp Gly Val Ile 115 120 125 Ala Ser Val Thr Pro Ser Thr Asn Pro Ser Ala Thr Ile Leu Asn Asn 130 135 140 Ala Ile Ser Met Ile Ala Ala Gly Asn Ser Val Val Phe Ser Pro His 145 150 155 160 Pro Ala Ala Arg Ala Val Ser Gln Arg Thr Ile Gln Leu Ile Asn Arg 165 170 175 Ala Ser Val Ser Ala Gly Gly Pro Ala Asn Leu Val Thr Cys Val Glu 180 185 190 Glu Pro Thr Ile Glu Ala Ala Thr Arg Leu Phe Ser Phe Pro Gly Ile 195 200 205 Gln Leu Leu Thr Ile Thr Gly Gly Glu Gly Val Val Asn Ala Ala Arg 210 215 220 Lys Val Thr Asp Lys Arg Leu Ile Ala Ala Gly Pro Gly Asn Pro Pro 225 230 235 240 Val Val Val Asp Glu Thr Ala Asp Ile Glu Arg Ala Ala Ile Ser Ile 245 250 255 Val Gln Gly Ala Ser Phe Asp Asn Asn Ile Ile Cys Val Asp Glu Lys 260 265 270 Glu Ile Ile Ala Val Glu Ser Ile Ala Thr Glu Leu Lys Thr Ala Met 275 280 285 Cys Arg His Gly Ala Ala Glu Ile Asn Ala Asp Gln Ala Asp Ala Val 290 295 300 Ala Arg Leu Val Leu Ala Gly Tyr Pro Gly Pro Asn Pro His Pro Lys 305 310 315 320 Pro Glu Trp Val Gly Arg Asp Ala Glu Lys Ile Ala Ala Ala Ala Gly 325 330 335 Phe Ser Val Pro Ala Gly Thr Arg Leu Leu Val Thr Glu Thr Glu Arg 340 345 350 Asp His Ala Phe Ala Thr Thr Glu Met Met Leu Pro Val Ile Ser Leu 355 360 365 Ile Arg Ala Arg Asp Ala Asp Gln Ala Ile Asp Trp Ala Val Glu Leu 370 375 380 Glu Ala Gly Asn Arg His Thr Ala Ala Met His Ser Arg Asn Ile Asp 385 390 395 400 Asn Leu Ser Arg Met Gly Leu Glu Ile Asn Cys Ser Leu Phe Val Lys 405 410 415 Asn Gly Pro Cys Leu Ala Gly Leu Gly Ala Gly Gly Glu Gly Trp Thr 420 425 430 Ser Met Thr Ile Ser Thr Pro Thr Gly Glu Gly Val Thr Asn Ala Ser 435 440 445 Thr Phe Val Arg Lys Arg Arg Cys Thr Met Val Asp Ser Phe Arg Ile 450 455 460 Val 465 <210> 82 <211> 473 <212> PRT <213> AADHma2 <400> 82 Met Ala Phe Asn Glu Asp Leu Ile Ala Ser Ile Val Glu Gln Val Leu 1 5 10 15 Lys Glu Ile Gln Asp Asp Asp His Val Ile Ala Ser Ala Arg Gln Ser 20 25 30 Val Val Lys Ala Asn Asp Asn Trp Gly Val Tyr Thr Asp Val Asp Glu 35 40 45 Ala Met Glu Val Val Asn Lys Ala Gln Lys Glu Phe Ala Ala Met Pro 50 55 60 Ile Ser Lys Arg Asp Glu Ile Ile Ser Ala Ile Arg Thr Ala Gly Val 65 70 75 80 Glu Asn Ala Glu His Leu Ala Arg Ile Ala His Glu Val Thr Gly Tyr 85 90 95 Gly Arg Ile Glu Asp Lys Thr Val Lys Asn Arg Asn Ala Ala Met Leu 100 105 110 Thr Pro Gly Ile Glu Asp Leu Gln Lys Glu Phe Leu Ser Gly Asp Gly 115 120 125 Gly Ser Val Ile Val Glu Tyr Gly Pro Met Gly Val Ile Ala Ser Leu 130 135 140 Met Pro Ser Thr His Pro Thr Ala Phe Val Ile Asn His Ala Ile Ala 145 150 155 160 Met Leu Ala Ala Gly Asn Ala Ile Phe Ile Cys Pro His Pro Lys Ala 165 170 175 Gln Ala Ala Thr Leu Glu Ala Met Lys Ala Leu Asn Lys Ala Ile Val 180 185 190 Glu Ala Gly Gly Pro Lys Tyr Leu Met Val Ala Val Asp Lys Ala Asp 195 200 205 Met Glu Thr Ser Asn Lys Val Tyr Ala His Pro Tyr Val Lys Met Val 210 215 220 Ala Ala Ala Gly Gly Glu Gly Val Ile Arg Ala Ala Leu Lys Ser Gly 225 230 235 240 Lys Lys Ala Ile Thr Gly Gly Pro Gly Asn Pro Pro Val Val Val Asp 245 250 255 Glu Thr Ala Asp Ile Ala Lys Ala Ala Lys Asp Ile Ile Asp Gly Glu 260 265 270 Ser Tyr Asp Asn Asn Val Leu Cys Ile Ala Glu Lys Glu Val Phe Val 275 280 285 Val Glu Ser Val Val Asp Arg Leu Ile Ala Glu Met Gln Lys Asn Asn 290 295 300 Ala Tyr Leu Ile Lys Gly Gln Asp Val Ala Asn Leu Thr Gly Val Thr 305 310 315 320 Ile Lys Asp Gly Lys Pro Asn Gly Gln Leu Val Gly Lys Asn Pro Ser 325 330 335 Val Ile Leu Lys Glu Ile Gly Ile Glu Val Gly Asp Asp Val Arg Thr 340 345 350 Val Ile Phe Glu Ala Pro Pro Glu His Pro Leu Val Met Ile Glu Gln 355 360 365 Leu Met Pro Val Leu Pro Ile Val Arg Val Lys Asp Phe Asp Glu Ala 370 375 380 Val Lys Trp Ala Val Lys Ala Glu His Gly Asp Gly His Thr Ala Ile 385 390 395 400 Leu His Ser Asn Asn Ile Asn Arg Ile Thr Glu Tyr Ala Arg Ala Met 405 410 415 Gln Val Thr Val Leu Val Val Asn Ala Pro Ser Tyr Ser Cys Met Asp 420 425 430 Pro Gln Ser Lys Thr Leu Val Tyr Ala His Thr Leu Thr Gly Pro Thr 435 440 445 Gly Glu Gly Phe Cys Thr Pro Arg His Phe Thr Arg Gln Glu Arg Ile 450 455 460 Val Leu Gly Gly Ala Phe His Phe Val 465 470 <210> 83 <211> 478 <212> PRT <213> AADHmm <400> 83 Met Asp Gln Lys Glu Ile Glu Asn Val Val Lys Ala Val Leu Ala Ser 1 5 10 15 Met Ser Ala Gly Thr Gln Pro Ala Ala Ala Ser Ala Ala Pro Gln Gln 20 25 30 Ala Ala Ala Ser Gln Asn Asn Gly Phe Gly Val Phe Glu Ser Leu Asp 35 40 45 Asp Ala Val Leu Ala Ala Lys Glu Ala Gln Lys Ser Leu Lys Thr Val 50 55 60 Glu Met Arg Asn Leu Cys Ile Gly Ala Ile Arg Arg Ala Ala Thr Glu 65 70 75 80 His Ala Arg Glu Leu Ala Val Leu Ala Val Glu Glu Thr Gly Met Gly 85 90 95 Arg Val Glu Asp Lys Leu Ala Lys Asn Leu Ala Gln Ala Asn Gly Thr 100 105 110 Pro Gly Val Glu Cys Leu Arg Pro Glu Val Leu Thr Gly Asp His Gly 115 120 125 Leu Thr Leu Ile Glu Asn Ala Ala Trp Gly Val Ile Ala Ser Val Thr 130 135 140 Pro Ser Thr Asn Pro Ala Ala Thr Ala Ile Asn Asn Ala Ile Ser Met 145 150 155 160 Ile Ala Gly Gly Asn Ser Val Ile Phe Ala Pro His Pro Ala Ala Lys 165 170 175 Lys Val Ser Gln Arg Thr Ile Thr Ile Leu Asn Glu Ala Ile Val Ala 180 185 190 Ala Gly Gly Pro Asn Asn Leu Leu Val Thr Val Ala Lys Pro Asp Ile 195 200 205 Glu Thr Ala Gln Arg Leu Phe Lys Tyr Pro Gly Ile Gly Leu Leu Val 210 215 220 Val Thr Gly Gly Asp Ala Val Val Glu Ser Ala Arg Lys His Thr Asn 225 230 235 240 Lys Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro Pro Val Val Val Asp 245 250 255 Glu Thr Ala Asp Ile Glu Arg Ala Ala Lys Ala Ile Val His Gly Ala 260 265 270 Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu Lys Val Leu Ile Ala 275 280 285 Val Asp Cys Ile Ala Asp Lys Leu Ile Thr Glu Met Gln Arg Asn His 290 295 300 Ala Val Leu Leu Thr Arg Glu Gln Ser Glu Lys Leu Ile Pro Val Leu 305 310 315 320 Leu Lys Asn Val Asp Glu Thr Gly His Gly Thr Val Ser Arg Asp Trp 325 330 335 Val Gly Arg Asp Ala Ala Lys Ile Ala Ala Ala Ile Gly Met Thr Val 340 345 350 Pro Ala Asp Thr Arg Leu Leu Ile Ala Glu Thr Asp Cys Lys His Pro 355 360 365 Phe Ala Val Thr Glu Leu Met Met Pro Val Leu Pro Ile Ile Arg Val 370 375 380 Lys Asp Val Asp Gln Ala Ile Asp Leu Ala Val Lys Leu Glu Gly Gly 385 390 395 400 Cys His His Thr Ala Ala Met His Ser Asn Asn Ile Ser Asn Leu Asn 405 410 415 Arg Met Ala Asn Ala Ile Asp Thr Ser Ile Phe Val Lys Asn Gly Pro 420 425 430 Cys Ile Ala Gly Leu Gly Leu Gly Gly Glu Gly Trp Thr Thr Met Thr 435 440 445 Ile Thr Thr Pro Thr Gly Glu Gly Val Thr Cys Ala Arg Thr Phe Val 450 455 460 Arg Leu Arg Arg Cys Thr Met Val Asp Ser Phe Arg Ile Val 465 470 475 <210> 84 <211> 477 <212> PRT <213> AADHpa <400> 84 Met Glu Ile Gln Asn Leu Glu Glu Asp Ile Arg Arg Ile Leu Ser Glu 1 5 10 15 Glu Leu Lys Lys Ser Gly Thr Ser Gln Thr Ala Ser Thr Ser Asp Ala 20 25 30 Gly Gln Asn Gly Ile Phe Lys Thr Val Asp Glu Ala Ile Ala Ala Ala 35 40 45 Lys Ala Ala Glu Asp Val Tyr Ile Asp Lys Thr Leu Ala Phe Arg Glu 50 55 60 Lys Val Leu Thr Ala Ile Arg Glu Gly Phe Arg Pro Tyr Ile Glu Lys 65 70 75 80 Met Ala Lys Asp Ile Lys Asp Glu Thr Gly Met Gly Thr Val Glu Ala 85 90 95 Lys Ile Ala Lys Leu Asn Asn Ala Leu Tyr Asn Thr Pro Gly Thr Glu 100 105 110 Ile Leu Gln Pro Glu Ala Glu Thr Gly Asp Gly Gly Leu Val Met Tyr 115 120 125 Glu Tyr Ala Pro Phe Gly Val Ile Gly Ala Val Gly Pro Ser Thr Asn 130 135 140 Pro Ser Glu Thr Val Ile Ala Asn Ala Ile Met Met Leu Ala Gly Gly 145 150 155 160 Asn Thr Leu Tyr Phe Gly Ala His Pro Gly Ala Lys Lys Ile Thr Arg 165 170 175 Trp Thr Ile Glu Lys Leu Asn Glu Leu Val Tyr Glu Ala Thr Gly Met 180 185 190 Lys Asn Leu Val Val Ser Ile Glu Glu Pro Ser Ile Glu Ser Val Gln 195 200 205 Glu Met Met Gln His Pro Asp Ile Ala Met Leu Ser Ile Thr Gly Gly 210 215 220 Pro Ala Val Val His Gln Ala Leu Val Ser Gly Lys Lys Ala Val Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ala Ile Val Asp Ala Thr Ala Asn Val 245 250 255 Ala Leu Ala Ala His Asn Ile Val Asp Ser Ala Ser Phe Asp Asn Asn 260 265 270 Ile Leu Cys Thr Ala Glu Lys Glu Val Val Val Glu Ser Ser Val Lys 275 280 285 Asp Glu Leu Ile Lys Lys Met Gln Glu Glu Gly Ala Phe Leu Val Thr 290 295 300 Asn Ala Ser Asp Ile Asp Lys Leu Ala Glu Met Thr Ile Gly Lys Asn 305 310 315 320 Gly Ala Pro Asp Arg Gln Phe Val Gly Lys Asp Ala Thr Tyr Ile Leu 325 330 335 Asp Lys Ala Gly Ile Ala Tyr Thr Gly Thr Pro Lys Leu Ile Ile Leu 340 345 350 Glu Ala Gln Lys Asp His Pro Leu Val Thr Thr Glu Met Leu Met Pro 355 360 365 Ile Val Pro Val Val Ser Cys Pro Thr Phe Asp Gln Val Leu Ala Thr 370 375 380 Ala Val Glu Val Glu Gln Gly Leu His His Thr Ala Ser Ile His Ser 385 390 395 400 Glu Asn Leu Pro Asn Ile Asn Arg Ala Ala His Arg Met Asn Thr Ser 405 410 415 Ile Phe Val Val Asn Gly Ala Thr Tyr Val Gly Thr Gly Val Gly Ala 420 425 430 Asn Gly Ala His Ala Gly Ala Ser Ala Leu Thr Ile Ala Thr Pro Thr 435 440 445 Gly Glu Gly Thr Ala Thr Ala Lys Thr Phe Thr Arg Arg Arg Arg Leu 450 455 460 Asn Ser Pro Glu Ala Phe Ser Leu Arg Ser Trp Glu Ala 465 470 475 <210> 85 <211> 477 <212> PRT <213> AADHpb <400> 85 Met Gln Thr Thr Glu Ala Thr Ile Arg Asn Val Val Gln Glu Val Leu 1 5 10 15 Ala Gln Leu Ala Lys Gln Ser Asn Gly Ser Asn Gly Val Pro Arg Ala 20 25 30 Glu Arg Lys Ala Gly Asp Trp Gly Val Phe Asp Asp Thr Asp Gln Ala 35 40 45 Ile Ala Ala Ala Asn Glu Gly Phe Arg Ala Leu Ser Ser Lys Gly Met 50 55 60 Glu Glu Arg Ala Lys Ile Val Glu Ile Val Lys Arg Ile Cys Ala Asp 65 70 75 80 Gln Ala Glu Glu Leu Gly Arg Leu Glu Phe Gln Glu Thr Lys Val Gly 85 90 95 Arg Leu Asp His Lys Ile Glu Lys Leu Gln Ile Ile Lys Lys Val Pro 100 105 110 Gly Thr Glu Trp Leu Arg Thr Glu Ala Met Ser Gly Asp His Gly Leu 115 120 125 Thr Val Ile Glu Tyr Ala Pro Phe Gly Val Ile Gly Thr Ile Thr Pro 130 135 140 Val Thr His Ser Leu Pro Thr Leu Ala Gly Asn Val Ile Asn Met Val 145 150 155 160 Ala Ala Gly Asn Thr Leu Val Val Asn Pro His Pro Ser Gly Lys Lys 165 170 175 Ile Ala Cys Glu Gly Val Arg Arg Phe Asn Arg Ala Ile Tyr Ala Ala 180 185 190 Thr Gly Leu Glu Asn Val Ile Thr Ile Val Ser Asn Pro Thr Leu Glu 195 200 205 Ser Ala Asp Arg Ile Phe Asn His Arg Asp Val Lys Met Leu Cys Val 210 215 220 Thr Gly Gly Pro Gly Val Ala Arg Ala Ala Met Ala Ser Arg Lys Lys 225 230 235 240 Ala Val Val Ala Gly Pro Gly Asn Pro Pro Val Leu Val Asp Glu Ser 245 250 255 Ala Cys Leu Gln Asn Ala Ala Glu Ser Ile Ile Ala Gly Ala Gly Tyr 260 265 270 Asp Asn Asn Leu Leu Cys Ile Gly Glu Lys Glu Val Phe Val Val Glu 275 280 285 Ser Val Phe Asp Arg Phe Met Gln Glu Met Asp Lys Ala Gly Ala Val 290 295 300 Arg Leu Asn Ala Ser Gln Val Asp Gln Leu Ser Lys Val Ala Phe Thr 305 310 315 320 Pro Pro Glu Asn Glu Lys Asp His Tyr His Val Asn Arg Asp Leu Val 325 330 335 Gly Gln His Pro Tyr Val Leu Ala Lys Gln Ile Gly Leu Pro Leu Pro 340 345 350 Glu Ser Thr Pro Leu Leu Phe Gly Glu Thr Asp Glu His Asn Pro Phe 355 360 365 Val Ala Glu Glu Gln Met Met Pro Phe Val Pro Ile Val Arg Cys Arg 370 375 380 Asn Ala Glu His Gly Met Asp Leu Cys Glu Lys Tyr Glu His Gly Phe 385 390 395 400 Arg His Thr Ser Leu Ile His Ser Arg Asn Val Arg Asn Met Ser Ile 405 410 415 Met Gly Lys Arg Met Asp Thr Thr Leu Phe Ile Lys Asn Gly Pro Ser 420 425 430 Pro Ala Gly Leu Gly Leu Gly Gly Glu Gly Tyr Leu Ser Phe Ser Ile 435 440 445 Ala Thr Pro Thr Gly Glu Gly Val Thr Thr Pro Leu Thr Phe Thr Arg 450 455 460 Gln Arg Arg Cys Thr Met Val Asp Asp Leu Arg Ile Val 465 470 475 <210> 86 <211> 452 <212> PRT <213> AADHpe <400> 86 Met Glu Ile Lys Glu Met Val Ala Leu Ala Arg Lys Ala Gln Lys Glu 1 5 10 15 Tyr Gln Ala Thr His Asn Gln Gln Ser Val Asp Asn Ile Cys Arg Ala 20 25 30 Ala Ala Lys Val Ile Tyr Glu Asn Ala Glu Met Leu Ala Arg Glu Ala 35 40 45 Val Asp Glu Thr Gly Met Gly Val Tyr Glu Asp Lys Val Ser Lys Asn 50 55 60 Arg Gly Lys Ser Lys Gly Val Trp Tyr Asn Leu His Asp Lys Lys Ser 65 70 75 80 Val Gly Ile Leu Asn Ile Asp Glu Arg Thr Gly Met Ile Glu Ile Ala 85 90 95 Lys Pro Met Gly Val Val Gly Ala Val Thr Pro Thr Thr Asn Pro Ile 100 105 110 Val Thr Pro Met Ser Asn Ile Ile Phe Ala Leu Lys Thr Cys Asn Ala 115 120 125 Ile Ile Ile Ser Pro His Pro Arg Ser Lys Lys Cys Ser Lys His Ala 130 135 140 Val Asp Leu Ile Leu Glu Ala Ile Lys Pro Phe Asn Val Pro Glu Gly 145 150 155 160 Met Val Gln Val Ile Glu Glu Pro Ser Ile Glu Met Thr Gln Gln Leu 165 170 175 Met Gly Ala Val Asp Val Val Val Ala Thr Gly Gly Met Ala Met Val 180 185 190 Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ser Tyr Gly Val Gly Ala Gly 195 200 205 Asn Val Gln Val Ile Phe Asp Ser Asp Ile Asp Phe Glu Asp Ala Ala 210 215 220 Glu Lys Val Ile Tyr Gly Arg Ser Tyr Asp Asn Gly Ile Ile Cys Thr 225 230 235 240 Gly Glu Gln Ser Val Ile Tyr Asn Glu Ala Asp Lys Asp Ala Val Phe 245 250 255 Ala Ala Leu Arg Lys Arg Gly Ala Tyr Ile Cys Thr Glu Glu Glu Gly 260 265 270 Lys Lys Met Arg Asp Leu Leu Phe Gln Asn Gly Gly Pro Ile Asn Lys 275 280 285 Asp Val Val Gly Gln Gly Val Gln Lys Ile Ala Lys Met Ala Gly Ile 290 295 300 Asn Val Pro Glu Gly Ser Arg Val Ile Ile Val Glu Ala Asn Gly Val 305 310 315 320 Gly Glu Glu Asp Val Ile Cys Arg Glu Lys Met Cys Pro Val Met Cys 325 330 335 Ala Leu Ser Tyr Lys His Phe Glu Glu Gly Val Asp Ile Ala Cys Arg 340 345 350 Asn Leu Ala Asn Glu Gly Asn Gly His Ser Cys Ala Ile His Ser Asn 355 360 365 Asn Gln Ala His Ile Ile Leu Thr Gly Ser Lys Ala Thr Val Ser Arg 370 375 380 Ile Val Val Asn Ala Pro Ser Ala Thr Thr Ala Gly Gly His Ile Gln 385 390 395 400 Asn Gly Leu Asn Val Thr Asn Thr Leu Gly Cys Gly Ser Trp Gly Asn 405 410 415 Asn Ser Ile Ser Glu Asn Phe Thr Tyr Lys His Leu Met Asn Ile Ser 420 425 430 Arg Ile Ala Pro Leu Asn Pro Ala Ile Lys Val Pro Asn Asp Gln Glu 435 440 445 Ile Trp Glu Leu 450 <210> 87 <211> 460 <212> PRT <213> AADHrw <400> 87 Met Thr Val Thr Ala Thr Leu Asp Ala Asp Leu Ala Ala Leu Ala Asp 1 5 10 15 Val Arg Ala Gln Val Ser Ala Thr Lys Lys Ala Phe Asp Ala Ile Glu 20 25 30 Gly Ala Asp Gln Gln Trp Leu Asp Glu Ile Val Arg Ala Met Ala Lys 35 40 45 Ala Gly Tyr Asp Ala Ser Glu Glu Leu Ala Arg Met Ala Val Asp Glu 50 55 60 Thr Gly Tyr Gly Val Tyr Glu Asn Lys Ile Ile Lys Asn Arg Phe Asn 65 70 75 80 Thr Gly Phe Val Ala Asp Trp Met Leu Glu Arg Arg Ala Val Gly Val 85 90 95 Leu Trp Ala Asp Glu Glu Asn Lys Val Thr Ala Val Gly Ser Pro Met 100 105 110 Gly Val Ile Ala Ala Ile Ile Pro Val Thr Asn Pro Thr Ala Thr Val 115 120 125 Leu Phe Lys Ser Leu Ala Ser Val Lys Ala Gly Asn Ala Val Val His 130 135 140 Ala Pro His Pro Arg Ala Ala Arg Cys Ser Ala Arg Ala Ala Glu Ile 145 150 155 160 Met Ala Glu Ala Ala Val His Ala Gly Ala Pro Glu Asn Leu Val Ser 165 170 175 Cys Leu Arg Gln Pro Thr Leu Asp Ala Thr Arg Glu Leu Met Arg His 180 185 190 Gly Asp Ile Arg Leu Val Leu Ala Thr Gly Gly Pro Ala Met Val Ala 195 200 205 Ala Ala Tyr Ser Ser Gly Lys Pro Thr Ile Ala Val Gly Ala Gly Asn 210 215 220 Val Pro Ala Tyr Val Asp Ser Thr Val Lys Asp Leu Ala Glu Thr Ala 225 230 235 240 Thr Met Ile Val Thr Ser Lys Ser Phe Asp Asn Gly Thr Ala Cys Val 245 250 255 Ala Glu Gln Ser Val Val Ile Ala Ala Pro Val Ala Asp Gln Leu Val 260 265 270 Gln Glu Leu Gly Arg Ala Gly Ala Thr Trp Met Thr Lys Asp Gln Gln 275 280 285 Ala Ala Leu Thr Arg Thr Leu Phe Asp Glu Arg Gly Gly Leu Arg Ala 290 295 300 Asn Ala Val Gly Gln Ser Ala Val Arg Leu Ala Glu Met Ser Gly Phe 305 310 315 320 Thr Val Pro Ala Ser Thr Lys Val Leu Ala Ala Glu Leu Asp Glu Val 325 330 335 Gly Asp His Val Leu Leu Ser Arg Glu Ile Leu Gly Pro Val Leu Thr 340 345 350 Val Tyr Arg Glu Pro Asp Tyr Gln Ala Gly Trp Arg Arg Cys Arg Glu 355 360 365 Ile Leu Ala Leu Gly Gly Glu Gly His Thr Val Ala Val His Thr Ser 370 375 380 Asp Pro Glu Val Val Ala Lys Phe Gly Glu Leu Pro Ala Gly Arg Ile 385 390 395 400 Val Val Asn Thr Pro Ala Leu Phe Gly Gly Met Gly Tyr Ser Thr Asn 405 410 415 Val Asp Pro Ser Phe Gln Ile Gly Thr Gly Thr Trp Ser Gly Ser Ile 420 425 430 Cys Ser Asp Asn Val Thr Pro Leu His Leu Val Asn Ile Lys Arg Ile 435 440 445 Ala His Glu Ala Arg Pro Trp Arg Gly Val Phe Asp 450 455 460 <210> 88 <211> 871 <212> PRT <213> AADHsl <400> 88 Met Ala Ile Glu Glu His Ser Leu Asn Asp Lys Glu Ser Lys Lys Glu 1 5 10 15 Thr Leu Glu Glu Thr Ile Asp Leu Leu Val Thr Asn Ala Lys Thr Ala 20 25 30 Ser Glu Glu Phe Thr Pro Leu Asp Gln Gln Gln Ile Asp Glu Ile Val 35 40 45 Arg Gln Met Ala Leu Ala Gly Leu Asp Gln His Met Gln Leu Ala Lys 50 55 60 Met Ala Val Glu Glu Thr Lys Arg Gly Val Tyr Glu Asp Lys Met Ile 65 70 75 80 Lys Asn Ile Phe Ala Thr Glu Tyr Ile Tyr Asn Ser Ile Lys Lys Asn 85 90 95 Lys Thr Val Gly Val Ile Ser Glu Asp Pro Tyr Glu Glu Ile Val Gln 100 105 110 Ile Ala Glu Pro Ile Gly Val Ile Ala Gly Val Thr Pro Val Thr Asn 115 120 125 Pro Thr Ser Thr Thr Met Phe Lys Ser Leu Ile Ser Ile Lys Thr Lys 130 135 140 Asn Pro Ile Ile Phe Ala Phe His Pro Ser Ala Gln Lys Cys Ser Ala 145 150 155 160 Ala Ala Ala Lys Val Val Arg Asp Ala Ala Val Lys Ala Gly Ala Pro 165 170 175 Lys Asn Cys Ile Gln Trp Ile Glu Thr Pro Ser Leu Glu Ala Thr Gln 180 185 190 Arg Leu Met His His Pro Gly Val Ser Leu Ile Leu Ala Thr Gly Gly 195 200 205 Ser Gly Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro Ala Ile Gly 210 215 220 Val Gly Pro Gly Asn Val Pro Ala Tyr Ile Glu Lys Ser Ala Asp Val 225 230 235 240 Lys Gln Ala Val Asn Asp Leu Ile Leu Ser Lys Thr Phe Asp Asn Gly 245 250 255 Met Ile Cys Ala Ser Glu Gln Ala Val Ile Val Asp Gln Gln Ile Tyr 260 265 270 Glu Ala Val Lys Lys Glu Met Ile Asp Asn Lys Cys Tyr Phe Leu Asn 275 280 285 Asn Glu Glu Lys Lys Lys Val Glu Lys Leu Val Ile Asp Glu Arg Arg 290 295 300 Cys Ala Val Asn Pro Gln Ile Val Gly Lys Thr Ala Ala Phe Ile Ala 305 310 315 320 Gly Leu Ala Gly Val Thr Val Pro Glu Asp Thr Lys Val Ile Val Ala 325 330 335 Glu Leu Ser Ser Val Gly Pro Glu Ser Pro Leu Ser Arg Glu Lys Leu 340 345 350 Ser Pro Val Leu Ala Cys Phe Arg Ala Lys Thr Thr Glu Glu Gly Phe 355 360 365 Lys Arg Cys Glu Glu Met Leu Ala Phe Gly Gly Ile Gly His Ser Ala 370 375 380 Val Ile His Ser Asn Asp Gln Lys Val Ile Asp Ala Phe Ala Met Arg 385 390 395 400 Met Lys Ala Gly Arg Leu Ile Val Asn Ala Pro Ser Ser Leu Gly Gly 405 410 415 Ile Gly Asp Ile Tyr Asn His Phe Ile Pro Ser Leu Thr Leu Gly Cys 420 425 430 Gly Ser Tyr Gly Gly Asn Ser Val Ser Thr Asn Val Gly Thr Ala Asn 435 440 445 Leu Ile Asn Ile Lys Thr Leu Ala Asn Arg Arg Asn Asn Met Gln Trp 450 455 460 Phe Lys Val Pro Pro Lys Ile Tyr Phe Glu Lys Asn Ala Thr Lys Tyr 465 470 475 480 Leu Ser Val Met Pro Asp Ile Thr Arg Ala Phe Ile Val Ala Asp Pro 485 490 495 Gly Met Val Lys Phe Gly Tyr Val Asp Arg Val Ile His Phe Leu Lys 500 505 510 Gln Arg Arg Asp Pro Val His Tyr Glu Val Phe Ser Asp Val Glu Pro 515 520 525 Asp Pro Ser Val Ala Thr Val Met Lys Gly Thr Glu Leu Met Arg Lys 530 535 540 Phe Glu Pro Asp Val Ile Ile Ala Leu Gly Gly Gly Ser Ala Met Asp 545 550 555 560 Ala Ala Lys Gly Met Trp Leu Phe Tyr Glu Ser Pro Glu Thr Asp Phe 565 570 575 Phe Gly Leu Lys Gln Lys Phe Leu Asp Ile Arg Lys Arg Val Phe Lys 580 585 590 Phe Pro Asp Leu Gly Lys Lys Ala Lys Phe Val Ala Ile Pro Thr Thr 595 600 605 Ser Gly Thr Gly Ser Glu Val Thr Ser Phe Ser Val Ile Thr Asp Lys 610 615 620 Glu Asn Asp Met Lys Tyr Pro Leu Ala Asp Tyr Ala Leu Thr Pro Asn 625 630 635 640 Val Ala Ile Ile Asp Pro Glu Phe Val Leu Thr Val Pro Gln Arg Val 645 650 655 Leu Ala Asp Ser Gly Met Asp Val Leu Thr His Ala Ile Glu Ala Tyr 660 665 670 Val Ser Asn Met Ala Asn Asp Phe Thr Asp Gly Leu Cys Ile Lys Ala 675 680 685 Ile Gln Leu Val Phe Lys Tyr Leu Pro Arg Ser Tyr Lys Asp Ser Thr 690 695 700 Asp Arg Glu Ala Lys Glu Lys Met His Asn Ala Ser Thr Ile Ala Gly 705 710 715 720 Met Ala Phe Ser Asn Ala Phe Leu Gly Ile Asn His Ser Leu Ala His 725 730 735 Lys Leu Gly Asn Glu Phe His Leu Ala His Gly Arg Cys Cys Ala Ile 740 745 750 Leu Leu Pro His Val Ile Arg Phe Asn Ala Gln Lys Pro Thr Lys Phe 755 760 765 Ala Ala Phe Pro Lys Tyr Lys His Phe Ile Ala Asp Glu Arg Tyr Ala 770 775 780 Glu Ile Ala Gln Val Leu Gly Ile Ala Gly Lys Thr Thr Glu Glu Ser 785 790 795 800 Val Glu Asn Leu Val Gln Ala Ile Lys Lys Leu Thr Gln Glu Leu Asn 805 810 815 Met Pro Met Ser Ile Glu Lys Asn Gly Val Ser Lys Glu Ala Phe Glu 820 825 830 Ala Ala Val Asp Lys Leu Ala Asp Arg Ala Phe Gly Asp Gln Cys Thr 835 840 845 Pro Ala Asn Pro Lys Met Pro Leu Val Ser Glu Leu Thr Glu Ile Tyr 850 855 860 Arg Gln Ala Phe Lys Gly Ile 865 870 <210> 89 <211> 462 <212> PRT <213> AADHss <400> 89 Met Ala Asp Glu Gln Leu Val Gln Asn Val Val Arg Glu Val Val Ala 1 5 10 15 Arg Met Gln Ile Ser Ala Pro Ala Arg Gly Met His Gly Val Phe Ser 20 25 30 Asp Met Glu Glu Ala Ile Glu Ala Ala Arg Thr Ala Gln Gln Thr Val 35 40 45 Arg Leu Leu Pro Met Asp Gln Arg Glu Lys Ile Ile Gly Ala Ile Arg 50 55 60 Arg Lys Thr Arg Glu Asn Ala Glu Ile Leu Ala Arg Met Ala Val Asn 65 70 75 80 Glu Thr Gly Met Gly Asn Val Gly Asp Lys Ile Leu Lys His Leu Leu 85 90 95 Val Ala Asp Lys Val Pro Gly Thr Glu Asp Ile Ser Thr Arg Ala Phe 100 105 110 Ser Gly Asp Arg Gly Leu Thr Leu Ile Glu Met Gly Pro Phe Gly Val 115 120 125 Ile Gly Ala Ile Thr Pro Cys Thr Asn Pro Ser Glu Thr Val Leu Cys 130 135 140 Asn Thr Ile Gly Met Leu Ala Gly Gly Asn Thr Val Val Phe Asn Pro 145 150 155 160 His Pro Gln Ala Ile Lys Thr Thr Leu Phe Thr Ile Gln Met Val Asn 165 170 175 Glu Ala Ser Leu Glu Ala Gly Gly Pro Asp Asn Ile Ala Cys Thr Val 180 185 190 Asp Ala Pro Thr Leu Ala Thr Ser Glu Ile Met Met Lys Ser Pro His 195 200 205 Ile Lys Leu Leu Val Ala Thr Gly Gly Pro Gly Val Val Thr Ala Val 210 215 220 Leu Ser Ser Gly Lys Arg Ala Ile Gly Ala Gly Ala Gly Asn Pro Pro 225 230 235 240 Ala Leu Val Asp Glu Thr Ala Asp Ile Arg Lys Ala Ala Glu Asp Ile 245 250 255 Val Asn Gly Cys Thr Phe Asp Asn Asn Leu Pro Cys Ile Ala Glu Lys 260 265 270 Glu Ile Val Ala Val Asp Ser Ile Ala Asp Glu Leu Leu His Tyr Met 275 280 285 Leu Thr Glu Gln Gly Cys Tyr Gln Ala Ser Glu Glu Glu Leu Asp Arg 290 295 300 Leu Thr Lys Ala Val Met Asp Glu Lys Gly Arg Leu Asn Arg Lys Ala 305 310 315 320 Val Gly Arg Ser Ala Arg Lys Leu Leu Ser Met Ile Gly Val Glu Val 325 330 335 Asp Ala Asn Ile Arg Cys Ile Thr Phe Phe Gly Pro Lys Glu His Pro 340 345 350 Leu Ile Thr Thr Glu Leu Met Met Pro Ile Leu Gly Ile Val Arg Val 355 360 365 Lys Asp Phe Ala Glu Gly Leu Glu Thr Ala Ala Trp Leu Glu His Gly 370 375 380 Asn Lys His Ser Ala His Ile His Ser Lys Asn Val Asp Arg Ile Thr 385 390 395 400 Glu Tyr Ala Arg Arg Leu Asp Thr Thr Ile Thr Val Lys Asn Gly Pro 405 410 415 Ser Tyr Ala Ala Leu Gly Phe Gly Gly Glu Ser Tyr Cys Thr Phe Thr 420 425 430 Ile Ala Ser Arg Thr Gly Glu Gly Leu Thr Ser Ala Arg Ser Phe Ile 435 440 445 Lys Ser Arg His Cys Val Met Thr Asp Ser Leu Cys Val Arg 450 455 460 <210> 90 <211> 467 <212> PRT <213> AADHta <400> 90 Met Asn Asn Thr Glu Leu Glu Ser Leu Ile Arg Thr Ile Leu Thr Glu 1 5 10 15 Gln Leu Thr Pro Ser Ala Thr Asp Thr Pro Ala Cys Thr Ala Ser Ser 20 25 30 Val Ala Leu Phe Asp Asp Val Asp Ser Ala Ile Cys Ala Ala His Ala 35 40 45 Ala Phe Leu Arg Tyr Gln Glu Ala Pro Leu Lys Thr Arg Ser Ala Ile 50 55 60 Ile Ala Ala Ile Arg Ala Glu Ile Ala Pro Cys Leu Ser Glu Leu Ala 65 70 75 80 Glu Arg Ala Ala Ala Glu Thr Gly Met Gly Asn Thr Ala Asp Lys Ile 85 90 95 Leu Lys Asn Lys Ala Ala Leu Glu Asn Thr Pro Gly Ile Glu Asp Leu 100 105 110 Lys Thr Thr Ala Leu Thr Gly Asp Glu Gly Met Val Leu Phe Glu Tyr 115 120 125 Ser Pro Phe Gly Val Val Gly Ala Val Ala Pro Ser Thr Asn Pro Thr 130 135 140 Glu Thr Ile Ile Asn Asn Ser Ile Ser Met Leu Ala Ala Gly Asn Ala 145 150 155 160 Ile Tyr Phe Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu Trp Leu 165 170 175 Ile Gln Lys Met Glu Glu Ile Ala Phe Lys Val Cys Gly Ile His Asn 180 185 190 Leu Ile Val Thr Val Lys Glu Pro Thr Phe Glu Ala Thr Gln Gln Met 195 200 205 Met Ala His Asp Lys Ile Ala Leu Leu Ala Ile Thr Gly Gly Pro Gly 210 215 220 Ile Val Asn Met Gly Leu Lys Ser Gly Lys Lys Val Ile Gly Ala Gly 225 230 235 240 Ala Gly Asn Pro Pro Cys Leu Val Asp Glu Thr Ala Glu Ile Val Lys 245 250 255 Ala Ala Gln Asp Ile Val Ala Gly Ala Ser Phe Asp Tyr Asn Leu Pro 260 265 270 Cys Ile Ala Glu Lys Ser Val Ile Ala Val Asp Cys Ile Ala Asp Gln 275 280 285 Leu Ile Gln Gln Met Arg Glu Phe Gly Ala Met Gln Ile Thr Asp Pro 290 295 300 Gln Gln Ile Ala Gln Leu Arg Glu Val Cys Ile Gln Lys Gly Ala Ala 305 310 315 320 Asn Lys Ser Leu Val Gly Lys Ser Pro Ala Thr Ile Leu Ala Ala Ala 325 330 335 Gly Ile Pro Cys Pro Ala Lys Glu Pro Arg Leu Ile Ile Leu Glu Val 340 345 350 Pro Ala Asn Asp Pro Phe Val Val Thr Glu Gln Leu Met Pro Val Leu 355 360 365 Pro Ile Val Arg Val Asp Asn Phe Glu Gln Gly Leu Gln Leu Ala Leu 370 375 380 Lys Val Glu Asp Gly Leu His His Thr Ala Met Met His Ser Gln Asn 385 390 395 400 Val Ser Arg Leu Asn Lys Ala Ala His Leu Met Gln Thr Ser Ile Phe 405 410 415 Val Lys Asn Gly Pro Ser Tyr Ala Gly Ile Gly Val Gly Ala Glu Gly 420 425 430 Phe Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser 435 440 445 Ala Arg Thr Phe Gly Arg Leu Arg Arg Cys Val Leu Thr Asn Gly Phe 450 455 460 Ser Ile Arg 465 <210> 91 <211> 474 <212> PRT <213> AADHtl <400> 91 Met Val Asp Ile Asp Leu Lys Asn Val Glu Gln Ile Val Arg Thr Val 1 5 10 15 Leu Ala Ser Met Gln Thr Gln Pro Gln Ala Val Gln Pro Ala Ala Gly 20 25 30 Arg Arg Ala Ala Pro Gly Val Phe Thr Glu Leu Asp Asp Ala Val Ala 35 40 45 Glu Ala Ser Thr Ala Gln Arg Ala Ile Arg Asn Leu Ala Thr Arg Val 50 55 60 Lys Val Val Glu Ala Ile Arg Lys Ala Ala Glu Ala His Ala Arg Glu 65 70 75 80 Leu Ala Glu Leu Ala Val Ala Glu Thr Gly Met Gly Arg Val Glu Asp 85 90 95 Lys Phe Ala Lys Asn Val Ser Gln Ala Arg His Thr Pro Gly Val Glu 100 105 110 Cys Leu Ala Pro Thr Ala Leu Thr Gly Asp Arg Gly Leu Thr Leu Ile 115 120 125 Glu Asn Ala Ala Trp Gly Val Ile Ala Ser Val Thr Pro Ser Thr Asn 130 135 140 Pro Ala Ala Thr Ile Ile Asn Asn Ala Ile Ser Met Ile Ser Ala Gly 145 150 155 160 Asn Thr Val Val Phe Ala Pro His Pro Ala Ala Lys Lys Val Ser Gln 165 170 175 Arg Thr Val Ser Leu Leu Asn Glu Ala Ser Val Ala Ala Gly Gly Pro 180 185 190 Asp Asn Val Val Thr Thr Val Leu Asn Pro Asp Ile Ala Thr Ala Gln 195 200 205 Arg Leu Phe Ser Tyr Pro Gly Ile His Leu Leu Thr Val Thr Gly Gly 210 215 220 Glu Ala Val Val Glu Ala Ala Arg Lys His Thr Asp Lys Arg Leu Ile 225 230 235 240 Ala Ala Gly Ala Gly Asn Pro Pro Val Val Val Asp Glu Thr Ala Asp 245 250 255 Leu Ala Arg Ala Ala Arg Asp Ile Val Trp Gly Ala Ser Phe Asp Asn 260 265 270 Asn Ile Ile Cys Val Asp Glu Lys Glu Ile Ile Val Val Asp Arg Val 275 280 285 Cys Asp Ala Leu Lys Thr Glu Met Ala Arg His Lys Ala Val Glu Leu 290 295 300 Thr Pro Asp Gln Gly Lys Arg Leu Val Asp Val Leu Leu Asn Tyr Val 305 310 315 320 Gly Ala Asp Gly Arg Gly Thr Val Ser Arg Asp Trp Val Gly Arg Asp 325 330 335 Ala Ala Lys Ile Ala Ala Ala Ile Gly Leu Glu Val Pro Ala Asp Thr 340 345 350 Arg Leu Leu Tyr Ile Glu Thr Pro Ala Asp His Pro Phe Ala Val Thr 355 360 365 Glu Leu Met Met Pro Val Ile Pro Val Val Arg Ala Pro Asp Val Asp 370 375 380 Ala Ala Ile Asp Leu Ala Val Lys Leu Glu Gly Asn Cys Arg His Thr 385 390 395 400 Ala Ala Met His Ser Arg Asn Ile Asp Ala Leu Asp Arg Met Ala Asn 405 410 415 Ala Ile Asn Thr Ser Ile Phe Val Lys Asn Gly Pro Cys Leu Ala Gly 420 425 430 Leu Gly Phe Gly Gly Glu Gly Trp Thr Thr Met Thr Ile Ser Thr Pro 435 440 445 Thr Gly Glu Gly Val Thr Ser Ala Arg Thr Phe Val Arg Leu Arg Arg 450 455 460 Cys Val Leu Val Asp His Phe Arg Ile Val 465 470 <210> 92 <211> 912 <212> PRT <213> AADHtm <400> 92 Met Asp Thr Ala Val Ala Val Gln Lys Gln Asp Ile Ala Met Ser Ala 1 5 10 15 Ala Ser Lys Pro Lys Ser Ala Ser Gly Pro Pro Phe Asp Val Gln Ala 20 25 30 His Val Asp Gly Leu Val Ala Arg Ala Ile Arg Ala Leu Ala Glu Phe 35 40 45 Gln Ala Leu Asp Gln Glu Gln Val Asp Arg Ile Val Ala Lys Ala Ser 50 55 60 Val Ala Ala Leu Asn Gln His Gly Val Leu Ala Lys Leu Ala Val Glu 65 70 75 80 Glu Thr Gly Arg Gly Leu Phe Glu Asp Lys Ala Val Lys Asn Met Phe 85 90 95 Ala Cys Glu Asn Val Thr Ser Ala Met Lys Arg Met Lys Thr Val Gly 100 105 110 Val Ile His Arg Asp Asn Leu Asn Gly Ile Val Glu Ile Ala Glu Pro 115 120 125 Val Gly Val Val Ala Ala Val Thr Pro Val Thr Asn Pro Thr Ser Thr 130 135 140 Ala Ile Phe Lys Ala Leu Val Cys Leu Lys Thr Arg Asn Pro Ile Val 145 150 155 160 Phe Gly Phe His Pro His Ala Gln Lys Cys Ser Ala Glu Ala Val Arg 165 170 175 Ile Val Arg Asp Ala Ala Val Ala Ala Gly Ala Pro Thr Asn Cys Ala 180 185 190 Gln Trp Ile Glu Val Pro Ser Ile Lys Ala Thr Gly Ala Leu Met Asn 195 200 205 His Pro Asp Ile Ser Val Ile Leu Ala Thr Gly Gly Asp Ala Met Val 210 215 220 Lys Ala Ala Tyr Ser Cys Gly Lys Pro Ala Ile Gly Val Gly Pro Gly 225 230 235 240 Asn Val Pro Ala Phe Val Glu Lys Thr Ala Asp Val Ala Arg Ala Ala 245 250 255 Tyr Asp Leu Val Leu Ser Lys Ser Phe Asp Tyr Gly Met Val Cys Ala 260 265 270 Ser Glu Gln Ala Ala Ile Ile Glu Ala Pro Val Tyr Asp Gln Ala Met 275 280 285 Ala Glu Met Gln Arg Leu His Ala His Val Ala Thr Ser Asp Glu Lys 290 295 300 Arg Lys Leu Glu Glu Leu Ala Phe Gly Val Val Ala Asn Ser Glu Asn 305 310 315 320 Cys Gly Ser Ala Lys Leu Asn Pro Ala Ile Val Gly Lys Ser Ala Ser 325 330 335 Trp Leu Ala Lys Gln Ala Gly Phe Ser Val Pro Gln Asp Thr Ser Ile 340 345 350 Leu Leu Val Glu Ala Asp His Val Gly Tyr Asp Glu Pro Leu Thr Arg 355 360 365 Glu Lys Leu Cys Pro Ile Leu Ala Val Ile Arg Ala Arg Asp Ala Asp 370 375 380 Asp Gly Ile Arg Leu Ser Glu Gln Met Val Glu Gln Asp Gly Leu Gly 385 390 395 400 His Ser Ala Cys Ile His Ser Thr Asp Glu Glu Val Ile Glu Lys Phe 405 410 415 Gly Arg Arg Ile Lys Ala Val Arg Ile Ile Thr Asn Ser Pro Thr Ser 420 425 430 Leu Gly Gly Ile Gly Asp Ile Tyr Asn Ala Phe Val Pro Ser Leu Thr 435 440 445 Leu Gly Cys Gly Thr Tyr Gly Lys Asn Ser Val Ser Asn Asn Val Gln 450 455 460 Ala Val Asn Leu Ile Asn Val Lys Arg Ile Gly His Arg Asn Asn Asn 465 470 475 480 Ile Gln Trp Phe Lys Val Pro Ala Lys Ile Tyr Phe Glu Pro Asn Ala 485 490 495 Ile Arg Tyr Leu Ala Asp Met Arg Arg Val Asn Arg Val Phe Ile Val 500 505 510 Thr Asp Ala Thr Met Ser Lys Leu Gly Val Val Asp Arg Ile Arg Asp 515 520 525 Val Leu Asn Arg Arg Leu Asn Met Val Thr Met Gln Val Val Asp Thr 530 535 540 Ile Asp Ser Glu Pro Ser Ile Ser Met Val Glu Val Gly Ala Glu Gln 545 550 555 560 Met Arg Asn Phe Gln Pro Asp Thr Ile Val Ala Leu Gly Gly Gly Ser 565 570 575 Ala Met Asp Ala Ala Lys Val Met Trp Leu Leu Tyr Glu His Pro Glu 580 585 590 Val Ser Phe Ser Asp Val Arg Glu Lys Cys Phe Asp Val Arg Lys Arg 595 600 605 Ala Phe Thr Phe Pro Asp Leu Gly Glu Lys Ala Gln Leu Val Cys Ile 610 615 620 Ser Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro Phe Ala Cys Ile 625 630 635 640 Thr Asp Pro Lys Thr Gly Tyr Lys Tyr Gln Leu Thr Asp Tyr Ala Leu 645 650 655 Met Pro Thr Val Ala Ile Val Asp Pro Thr Leu Val Asn Ala Met Pro 660 665 670 Ser Arg Leu Ala Ala Asp Ser Gly Met Asp Ala Leu Ala Arg Ala Thr 675 680 685 Glu Ala Tyr Val Ser Val Tyr Ala Asn Asp Phe Thr Asp Gly Leu Ala 690 695 700 Met Gln Ala Ile Lys Leu Ile Phe Glu Asn Leu Pro Ala Ser Val Leu 705 710 715 720 Lys Gly Asp Ala Lys Ala Arg Glu Lys Met His Asn Ala Gly Thr Ile 725 730 735 Ala Gly Met Ala Ser Gly Ser Ala Phe Leu Gly Ile Val His Ala Ile 740 745 750 Thr His Thr Ile Gly Ala Ser Tyr Arg Ile Ser Gln Gly Arg Ser Asn 755 760 765 Ala Thr Leu Leu Pro Leu Gly Ile Arg Tyr Asn Gly Arg Ile Pro Thr 770 775 780 Lys Leu Thr Ser Trp Pro Lys Tyr Glu Lys Tyr Val Ala Pro Gln Arg 785 790 795 800 Tyr Gln Asp Ile Ala Lys Met Leu Gly Leu Pro Ala Ala Thr Pro Glu 805 810 815 Glu Gly Val Glu Ser Tyr Ala Arg Ala Val Glu Asp Leu Arg Ala Thr 820 825 830 Leu Asn Met Glu Tyr Ser Phe Arg Ala Gln Gly Ile Glu Glu His Asp 835 840 845 Phe Val Ala Asn Leu Asp Glu Met Ala Phe Arg Ala Ser Asp Asp Gln 850 855 860 Ser Ala Pro Ala Asn Pro Arg Leu Pro Met Val Glu Asp Leu Lys Ile 865 870 875 880 Ile Met Glu Ala Ala Tyr Tyr Gly Ile Ser Phe Asp Asp Ile Val Ala 885 890 895 Arg Arg Ala Ser Ala Gln Leu Ala Lys Ser Glu Val Ser Lys Lys Gly 900 905 910 <210> 93 <211> 532 <212> PRT <213> AADHvs <400> 93 Met Asn Glu Gln Glu Ile Ala His Ala Val Glu Asn Val Leu Ser Lys 1 5 10 15 Tyr Thr Asn Val Thr Ala Gln Asn Ala Glu Pro Val Ser Tyr Ser Ser 20 25 30 Asn Ala Ser Leu Glu Asn Ile Val Ser Gln Ala Leu Ala Gly Asn Met 35 40 45 Val Lys Gln Pro Glu Thr Gln Thr Ala Pro Asp Leu Asn Ser Asn Ile 50 55 60 Glu Asn Ile Val Ser Gln Ile Leu Ala Glu Asn Gln Ala Lys Pro Gln 65 70 75 80 Ser Val Gln Cys Gln Ser Ala Asn His Gly Thr Thr Glu Tyr Leu Gly 85 90 95 Cys Phe Ala Ser Met Glu Glu Ala Ile Ser Ala Ala Ser His Ala Gln 100 105 110 Val Gln Tyr Arg His Cys Thr Met Gly Asp Arg Ala Ser Phe Val Lys 115 120 125 Gly Ile Arg Glu Val Phe Thr Gln Asp Asp Val Leu Glu Lys Ile Ser 130 135 140 Arg Met Ala Val Glu Glu Thr Gly Met Gly Asn Tyr Ala Asp Lys Leu 145 150 155 160 Thr Lys Asn Arg Ile Ala Ala Thr Lys Thr Pro Gly Ile Glu Asp Leu 165 170 175 Thr Thr Ser Ala Leu Ser Gly Asp Ser Gly Leu Thr Leu Thr Glu Phe 180 185 190 Ser Ala Tyr Gly Val Ile Gly Ser Ile Thr Pro Thr Thr Asn Pro Thr 195 200 205 Glu Thr Ile Ile Asn Asn Ser Ile Gly Met Leu Ala Ala Gly Asn Thr 210 215 220 Val Val Tyr Ser Pro His Pro Arg Ser Arg Asn Val Ser Leu Val Ala 225 230 235 240 Val Asp Leu Ile Asn Arg Lys Leu Ala Glu Leu Gly Ala Pro Ala Asn 245 250 255 Leu Val Val Thr Val Leu Glu Pro Ser Ile Asp Asn Thr Asn Ala Met 260 265 270 Met Asn Asp Pro Arg Val Asn Met Leu Val Ala Thr Gly Gly Pro Ser 275 280 285 Ile Val Lys Thr Val Met Ser Thr Gly Lys Lys Ala Ile Gly Ala Gly 290 295 300 Ala Gly Asn Pro Pro Ala Val Val Asp Glu Thr Ala Asn Ile Glu Lys 305 310 315 320 Ala Ala Lys Asp Ile Ile Asn Gly Cys Ala Phe Asp Asn Asn Leu Pro 325 330 335 Cys Ile Ala Glu Lys Glu Val Ile Val Val Asn Glu Val Ala Asp Tyr 340 345 350 Leu Ile His Cys Met Lys Lys Ser Gly Ala Tyr Leu Leu Cys Asp Lys 355 360 365 Gln Lys Ile Gln Gln Leu Gln Ser Leu Val Leu Asn Glu Lys Gly Thr 370 375 380 Gly Pro Asn Thr Ser Phe Val Gly Lys Gly Ala Arg Tyr Ile Leu Asp 385 390 395 400 Lys Leu Asn Ile Gln Val Ser Asp Asp Ile Lys Val Ile Leu Ile Glu 405 410 415 Thr Glu Arg Asn His Pro Phe Val Val His Glu Leu Met Met Pro Ile 420 425 430 Leu Pro Val Val Arg Val Glu Asn Val Asp Glu Ala Ile Asp Leu Ala 435 440 445 Ile Lys Val Glu His Gly Asn Arg His Thr Ala Ile Met His Ser Thr 450 455 460 Asn Val Glu Lys Leu Ser Lys Met Ala Arg Leu Ile Gln Thr Thr Ile 465 470 475 480 Phe Val Lys Asn Gly Pro Ser Tyr Ser Gly Ile Gly Val Gly Gly Glu 485 490 495 Gly His Thr Thr Phe Thr Ile Ala Gly Pro Thr Gly Glu Gly Ile Thr 500 505 510 Ser Ala Arg Ser Phe Ala Arg Tyr Arg Arg Cys Val Met Val Glu Ala 515 520 525 Leu Asn Ile Arg 530 <210> 94 <211> 891 <212> PRT <213> ADHEec <400> 94 Met Ala Val Thr Asn Val Ala Glu Leu Asn Ala Leu Val Glu Arg Val 1 5 10 15 Lys Lys Ala Gln Arg Glu Tyr Ala Ser Phe Thr Gln Glu Gln Val Asp 20 25 30 Lys Ile Phe Arg Ala Ala Ala Leu Ala Ala Ala Asp Ala Arg Ile Pro 35 40 45 Leu Ala Lys Met Ala Val Ala Glu Ser Gly Met Gly Ile Val Glu Asp 50 55 60 Lys Val Ile Lys Asn His Phe Ala Ser Glu Tyr Ile Tyr Asn Ala Tyr 65 70 75 80 Lys Asp Glu Lys Thr Cys Gly Val Leu Ser Glu Asp Asp Thr Phe Gly 85 90 95 Thr Ile Thr Ile Ala Glu Pro Ile Gly Ile Ile Cys Gly Ile Val Pro 100 105 110 Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys Ser Leu Ile Ser Leu 115 120 125 Lys Thr Arg Asn Ala Ile Ile Phe Ser Pro His Pro Arg Ala Lys Asp 130 135 140 Ala Thr Asn Lys Ala Ala Asp Ile Val Leu Gln Ala Ala Ile Ala Ala 145 150 155 160 Gly Ala Pro Lys Asp Leu Ile Gly Trp Ile Asp Gln Pro Ser Val Glu 165 170 175 Leu Ser Asn Ala Leu Met His His Pro Asp Ile Asn Leu Ile Leu Ala 180 185 190 Thr Gly Gly Pro Gly Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro 195 200 205 Ala Ile Gly Val Gly Ala Gly Asn Thr Pro Val Val Ile Asp Glu Thr 210 215 220 Ala Asp Ile Lys Arg Ala Val Ala Ser Val Leu Met Ser Lys Thr Phe 225 230 235 240 Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ser Val Val Val Val Asp 245 250 255 Ser Val Tyr Asp Ala Val Arg Glu Arg Phe Ala Thr His Gly Gly Tyr 260 265 270 Leu Leu Gln Gly Lys Glu Leu Lys Ala Val Gln Asp Val Ile Leu Lys 275 280 285 Asn Gly Ala Leu Asn Ala Ala Ile Val Gly Gln Pro Ala Tyr Lys Ile 290 295 300 Ala Glu Leu Ala Gly Phe Ser Val Pro Glu Asn Thr Lys Ile Leu Ile 305 310 315 320 Gly Glu Val Thr Val Val Asp Glu Ser Glu Pro Phe Ala His Glu Lys 325 330 335 Leu Ser Pro Thr Leu Ala Met Tyr Arg Ala Lys Asp Phe Glu Asp Ala 340 345 350 Val Glu Lys Ala Glu Lys Leu Val Ala Met Gly Gly Ile Gly His Thr 355 360 365 Ser Cys Leu Tyr Thr Asp Gln Asp Asn Gln Pro Ala Arg Val Ser Tyr 370 375 380 Phe Gly Gln Lys Met Lys Thr Ala Arg Ile Leu Ile Asn Thr Pro Ala 385 390 395 400 Ser Gln Gly Gly Ile Gly Asp Leu Tyr Asn Phe Lys Leu Ala Pro Ser 405 410 415 Leu Thr Leu Gly Cys Gly Ser Trp Gly Gly Asn Ser Ile Ser Glu Asn 420 425 430 Val Gly Pro Lys His Leu Ile Asn Lys Lys Thr Val Ala Lys Arg Ala 435 440 445 Glu Asn Met Leu Trp His Lys Leu Pro Lys Ser Ile Tyr Phe Arg Arg 450 455 460 Gly Ser Leu Pro Ile Ala Leu Asp Glu Val Ile Thr Asp Gly His Lys 465 470 475 480 Arg Ala Leu Ile Val Thr Asp Arg Phe Leu Phe Asn Asn Gly Tyr Ala 485 490 495 Asp Gln Ile Thr Ser Val Leu Lys Ala Ala Gly Val Glu Thr Glu Val 500 505 510 Phe Phe Glu Val Glu Ala Asp Pro Thr Leu Ser Ile Val Arg Lys Gly 515 520 525 Ala Glu Leu Ala Asn Ser Phe Lys Pro Asp Val Ile Ile Ala Leu Gly 530 535 540 Gly Gly Ser Pro Met Asp Ala Ala Lys Ile Met Trp Val Met Tyr Glu 545 550 555 560 His Pro Glu Thr His Phe Glu Glu Leu Ala Leu Arg Phe Met Asp Ile 565 570 575 Arg Lys Arg Ile Tyr Lys Phe Pro Lys Met Gly Val Lys Ala Lys Met 580 585 590 Ile Ala Val Thr Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro Phe 595 600 605 Ala Val Val Thr Asp Asp Ala Thr Gly Gln Lys Tyr Pro Leu Ala Asp 610 615 620 Tyr Ala Leu Thr Pro Asp Met Ala Ile Val Asp Ala Asn Leu Val Met 625 630 635 640 Asp Met Pro Lys Ser Leu Cys Ala Phe Gly Gly Leu Asp Ala Val Thr 645 650 655 His Ala Met Glu Ala Tyr Val Ser Val Leu Ala Ser Glu Phe Ser Asp 660 665 670 Gly Gln Ala Leu Gln Ala Leu Lys Leu Leu Lys Glu Tyr Leu Pro Ala 675 680 685 Ser Tyr His Glu Gly Ser Lys Asn Pro Val Ala Arg Glu Arg Val His 690 695 700 Ser Ala Ala Thr Ile Ala Gly Ile Ala Phe Ala Asn Ala Phe Leu Gly 705 710 715 720 Val Cys His Ser Met Ala His Lys Leu Gly Ser Gln Phe His Ile Pro 725 730 735 His Gly Leu Ala Asn Ala Leu Leu Ile Cys Asn Val Ile Arg Tyr Asn 740 745 750 Ala Asn Asp Asn Pro Thr Lys Gln Thr Ala Phe Ser Gln Tyr Asp Arg 755 760 765 Pro Gln Ala Arg Arg Arg Tyr Ala Glu Ile Ala Asp His Leu Gly Leu 770 775 780 Ser Ala Pro Gly Asp Arg Thr Ala Ala Lys Ile Glu Lys Leu Leu Ala 785 790 795 800 Trp Leu Glu Thr Leu Lys Ala Glu Leu Gly Ile Pro Lys Ser Ile Arg 805 810 815 Glu Ala Gly Val Gln Glu Ala Asp Phe Leu Ala Asn Val Asp Lys Leu 820 825 830 Ser Glu Asp Ala Phe Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Tyr 835 840 845 Pro Leu Ile Ser Glu Leu Lys Gln Ile Leu Leu Asp Thr Tyr Tyr Gly 850 855 860 Arg Asp Tyr Val Glu Gly Glu Thr Ala Ala Lys Lys Glu Ala Ala Pro 865 870 875 880 Ala Lys Ala Glu Lys Lys Ala Lys Lys Ser Ala 885 890 <210> 95 <211> 885 <212> PRT <213> ADHEpm <400> 95 Met Ser Gly Leu Gln Met Phe Gln Asn Leu Ser Leu Tyr Gly Ser Leu 1 5 10 15 Ala Glu Ile Asp Thr Ser Glu Lys Leu Asn Glu Ala Met Asp Lys Leu 20 25 30 Thr Ala Ala Gln Glu Gln Phe Arg Glu Tyr Asn Gln Glu Gln Val Asp 35 40 45 Lys Ile Phe Lys Ala Val Ala Leu Ala Ala Ser Gln Asn Arg Val Ala 50 55 60 Phe Ala Lys Tyr Ala His Glu Glu Thr Gln Lys Gly Val Phe Glu Asp 65 70 75 80 Lys Val Ile Lys Asn Glu Phe Ala Ala Asp Tyr Ile Tyr His Lys Tyr 85 90 95 Cys Asn Asp Lys Thr Ala Gly Ile Ile Glu Tyr Asp Glu Ala Asn Gly 100 105 110 Leu Met Glu Ile Ala Glu Pro Val Gly Pro Val Val Gly Ile Ala Pro 115 120 125 Val Thr Asn Pro Thr Ser Thr Ile Ile Tyr Lys Ser Leu Ile Ala Leu 130 135 140 Lys Thr Arg Asn Cys Ile Ile Phe Ser Pro His Pro Gly Ala His Lys 145 150 155 160 Ala Ser Val Phe Val Val Lys Val Leu His Gln Ala Ala Val Lys Ala 165 170 175 Gly Ala Pro Glu Asn Cys Ile Gln Ile Ile Phe Pro Lys Met Asp Leu 180 185 190 Thr Thr Glu Leu Leu His His Gln Lys Thr Arg Phe Ile Trp Ala Thr 195 200 205 Gly Gly Pro Gly Leu Val His Ala Ser Tyr Thr Ser Gly Lys Pro Ala 210 215 220 Leu Gly Gly Gly Pro Gly Asn Ala Pro Ala Leu Ile Asp Glu Thr Cys 225 230 235 240 Asp Met Asn Glu Ala Val Gly Ser Ile Val Val Ser Lys Thr Phe Asp 245 250 255 Cys Gly Met Ile Cys Ala Thr Glu Asn Ala Val Val Val Val Glu Ser 260 265 270 Val Tyr Glu Asn Phe Val Ala Thr Met Lys Lys Arg Gly Ala Tyr Phe 275 280 285 Met Thr Pro Glu Glu Thr Lys Lys Ala Ser Asn Leu Leu Phe Gly Glu 290 295 300 Gly Met Arg Leu Asn Ala Lys Ala Val Gly Gln Thr Ala Lys Thr Leu 305 310 315 320 Ala Glu Met Ala Gly Phe Glu Val Pro Glu Asn Thr Val Val Leu Cys 325 330 335 Gly Glu Ala Ser Glu Val Lys Phe Glu Glu Pro Met Ala His Glu Lys 340 345 350 Leu Thr Thr Ile Leu Gly Ile Tyr Lys Ala Lys Asp Phe Asp Asp Gly 355 360 365 Val Arg Leu Cys Lys Glu Leu Val Thr Phe Gly Gly Lys Gly His Thr 370 375 380 Ala Val Leu Tyr Thr Asn Gln Asn Asn Lys Asp Arg Ile Glu Lys Tyr 385 390 395 400 Gln Asn Glu Val Pro Ala Phe His Ile Leu Val Asp Met Pro Ser Ser 405 410 415 Leu Gly Cys Ile Gly Asp Met Tyr Asn Phe Arg Leu Ala Pro Ala Leu 420 425 430 Thr Ile Thr Cys Gly Thr Met Gly Gly Gly Ser Ser Ser Asp Asn Ile 435 440 445 Gly Pro Lys His Leu Leu Asn Ile Lys Arg Val Gly Met Arg Arg Glu 450 455 460 Asn Met Leu Trp Phe Lys Ile Pro Lys Ser Val Tyr Phe Lys Arg Ala 465 470 475 480 Ile Leu Ser Glu Ala Leu Ser Asp Leu Arg Asp Thr His Lys Arg Ala 485 490 495 Ile Ile Ile Thr Asp Arg Thr Met Thr Met Leu Gly Gln Thr Asp Lys 500 505 510 Ile Ile Lys Ala Cys Glu Gly His Gly Met Val Cys Thr Val Tyr Asp 515 520 525 Lys Val Val Pro Asp Pro Thr Ile Lys Cys Ile Met Glu Gly Val Asn 530 535 540 Glu Met Asn Val Phe Lys Pro Asp Leu Ala Ile Ala Leu Gly Gly Gly 545 550 555 560 Ser Ala Met Asp Ala Ala Lys Met Met Arg Leu Phe Tyr Glu Tyr Pro 565 570 575 Asp Gln Asp Leu Gln Asp Ile Ala Thr Arg Phe Val Asp Ile Arg Lys 580 585 590 Arg Val Val Gly Cys Pro Lys Leu Gly Arg Leu Ile Lys Thr Leu Val 595 600 605 Cys Ile Pro Thr Thr Ser Gly Thr Gly Ala Glu Val Thr Pro Phe Ala 610 615 620 Val Val Thr Ser Glu Glu Gly Arg Lys Tyr Pro Leu Val Asp Tyr Glu 625 630 635 640 Leu Thr Pro Asp Met Ala Ile Val Asp Pro Glu Phe Ala Val Gly Met 645 650 655 Pro Lys Arg Leu Thr Ser Trp Thr Gly Ile Asp Ala Leu Thr His Ala 660 665 670 Ile Glu Ser Tyr Val Ser Ile Met Ala Thr Asp Phe Thr Arg Pro Tyr 675 680 685 Ser Leu Arg Ala Val Gly Leu Ile Phe Glu Ser Leu Ser Leu Ala Tyr 690 695 700 Asn Asn Gly Lys Asp Ile Glu Ala Arg Glu Lys Met His Asn Ala Ser 705 710 715 720 Ala Ile Ala Gly Met Ala Phe Ala Asn Ala Phe Leu Gly Cys Cys His 725 730 735 Ser Val Ala His Gln Leu Gly Ser Val Tyr His Ile Pro His Gly Leu 740 745 750 Ala Asn Ala Leu Met Leu Ser His Ile Ile Lys Tyr Asn Ala Thr Asp 755 760 765 Ser Pro Val Lys Met Gly Thr Phe Pro Gln Tyr Lys Tyr Pro Gln Ala 770 775 780 Met Arg His Tyr Ala Glu Ile Ala Glu Leu Leu Leu Pro Pro Thr Gln 785 790 795 800 Val Val Lys Met Thr Asp Val Asp Lys Val Gln Tyr Leu Ile Asp Arg 805 810 815 Val Glu Gln Leu Lys Ala Asp Val Gly Ile Pro Lys Ser Ile Lys Glu 820 825 830 Thr Gly Met Val Thr Glu Glu Asp Phe Phe Asn Lys Val Asp Gln Val 835 840 845 Ala Ile Met Ala Phe Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Tyr 850 855 860 Pro Leu Val Ser Glu Leu Lys Gln Leu Met Ile Asp Ala Trp Asn Gly 865 870 875 880 Val Val Pro Lys Leu 885 <210> 96 <211> 459 <212> PRT <213> EUTEdz <400> 96 Met Glu His Ser Val Ile Glu Pro Thr Val Pro Met Pro Leu Pro Ala 1 5 10 15 Met Phe Asp Ala Pro Ser Gly Ile Phe Ser Ser Leu Asp Asp Ala Val 20 25 30 Gln Ala Ala Thr Leu Ala Gln Gln Gln Leu Ser Ser Val Glu Leu Arg 35 40 45 Gln Gln Val Ile Lys Ala Ile Arg Val Ala Gly Glu Arg Tyr Ala Gln 50 55 60 Val Leu Ala Glu Met Ala Val Ala Glu Thr Gly Met Gly Arg Val Val 65 70 75 80 Asp Lys Tyr Ile Lys Asn Val Ser Gln Ala Arg His Thr Pro Gly Ile 85 90 95 Glu Cys Leu Ser Ala Glu Val Leu Thr Gly Asp Asn Gly Leu Thr Leu 100 105 110 Ile Glu Asn Ala Pro Trp Gly Val Val Ala Ser Val Thr Pro Ser Thr 115 120 125 Asn Pro Ala Ala Thr Val Ile Asn Asn Ala Ile Ser Met Ile Ala Ala 130 135 140 Gly Asn Ser Val Val Phe Ala Pro His Pro Ser Ala Lys Asn Val Ser 145 150 155 160 Leu Arg Thr Ile Ser Leu Leu Asn Lys Ala Ile Val Ala Thr Gly Gly 165 170 175 Pro Glu Asn Leu Leu Val Ser Val Ala Asn Pro Asn Ile Glu Thr Ala 180 185 190 Gln Arg Leu Phe Arg Tyr Pro Gly Ile Gly Leu Leu Val Val Thr Gly 195 200 205 Gly Glu Ala Val Val Glu Ala Ala Arg Lys His Thr Asp Lys Arg Leu 210 215 220 Ile Ala Ala Gly Ala Gly Asn Pro Pro Val Val Val Asp Glu Thr Ala 225 230 235 240 Asp Ile Pro Lys Ala Ala Arg Ala Ile Val Lys Gly Ala Ser Phe Asp 245 250 255 Asn Asn Ile Ile Cys Ala Asp Glu Lys Val Leu Ile Val Val Asp Arg 260 265 270 Val Ala Asp Ala Leu Leu Ala Glu Met Gln Arg Asn Asn Ala Val Leu 275 280 285 Leu Thr Pro Glu Gln Thr Glu Arg Leu Leu Pro Ala Leu Leu Ser Asp 290 295 300 Ile Asp Glu Gln Gly Lys Gly Arg Val Asn Arg Asp Tyr Val Gly Arg 305 310 315 320 Asp Ala Ala Lys Leu Ala Ala Ala Ile Gly Leu Glu Val Ser Glu His 325 330 335 Thr Arg Leu Leu Leu Ala Glu Thr Asp Ala Asp His Pro Phe Ala Val 340 345 350 Thr Glu Leu Met Met Pro Val Leu Pro Val Ile Arg Val Lys Asn Val 355 360 365 Asp Asp Ala Ile Ala Leu Ala Val Lys Leu Glu Ser Gly Cys Arg His 370 375 380 Thr Ala Ala Met His Ser Thr Asn Ile Arg Asn Leu Asn Arg Met Ala 385 390 395 400 Asn Ala Ile Asn Thr Ser Ile Phe Val Lys Asn Gly Pro Cys Ile Ala 405 410 415 Gly Leu Gly Leu Gly Gly Glu Gly Trp Thr Ser Met Thr Ile Ser Thr 420 425 430 Pro Thr Gly Glu Gly Val Thr Ser Ala Arg Thr Phe Val Arg Leu Arg 435 440 445 Arg Cys Val Leu Val Asp Met Phe Arg Ile Ala 450 455 <210> 97 <211> 467 <212> PRT <213> EUTEec <400> 97 Met Asn Gln Gln Asp Ile Glu Gln Val Val Lys Ala Val Leu Leu Lys 1 5 10 15 Met Gln Ser Ser Asp Thr Pro Pro Ala Ala Val His Glu Met Gly Val 20 25 30 Phe Ala Ser Leu Asp Asp Ala Val Ala Ala Ala Lys Val Ala Gln Gln 35 40 45 Gly Leu Lys Ser Val Ala Met Arg Gln Leu Ala Ile Ala Ala Ile Arg 50 55 60 Glu Ala Gly Glu Lys His Ala Arg Asp Leu Ala Glu Leu Ala Val Ser 65 70 75 80 Glu Thr Gly Met Gly Arg Val Glu Asp Lys Phe Ala Lys Asn Val Ala 85 90 95 Gln Ala Arg Gly Thr Pro Gly Val Glu Cys Leu Ser Pro Gln Val Leu 100 105 110 Thr Gly Asp Asn Gly Leu Thr Leu Ile Glu Asn Ala Pro Trp Gly Val 115 120 125 Val Ala Ser Val Thr Pro Ser Thr Asn Pro Ala Ala Thr Leu Ile Asn 130 135 140 Asn Ala Ile Ser Leu Ile Ala Ala Gly Asn Ser Val Ile Phe Ala Pro 145 150 155 160 His Pro Ala Ala Lys Lys Val Ser Gln Arg Ala Ile Thr Leu Leu Asn 165 170 175 Gln Ala Ile Val Ala Ala Gly Gly Pro Glu Asn Leu Leu Val Thr Val 180 185 190 Ala Asn Pro Asp Ile Glu Thr Ala Gln Arg Leu Phe Lys Phe Pro Gly 195 200 205 Ile Gly Leu Leu Val Val Thr Gly Gly Glu Ala Val Val Glu Ala Ala 210 215 220 Arg Lys His Thr Asn Lys Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro 225 230 235 240 Pro Val Val Val Asp Glu Thr Ala Asp Leu Ala Arg Ala Ala Gln Ser 245 250 255 Ile Val Lys Gly Ala Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu 260 265 270 Lys Val Leu Ile Val Val Asp Ser Val Ala Asp Glu Leu Met Arg Leu 275 280 285 Met Glu Gly Gln His Ala Val Lys Leu Thr Ala Glu Gln Ala Gln Gln 290 295 300 Leu Gln Pro Val Leu Leu Lys Asn Ile Asp Glu Arg Gly Lys Gly Thr 305 310 315 320 Val Ser Arg Asp Trp Val Gly Arg Asp Ala Ala Lys Ile Ala Ala Ala 325 330 335 Ile Gly Leu Asn Val Pro Gln Glu Thr Arg Leu Leu Phe Val Glu Thr 340 345 350 Thr Ala Glu His Pro Phe Ala Val Thr Glu Leu Met Met Pro Val Leu 355 360 365 Pro Val Val Arg Val Ala Asn Val Ala Asp Ala Ile Ala Leu Ala Val 370 375 380 Lys Leu Glu Gly Gly Cys His His Thr Ala Ala Met His Ser Arg Asn 385 390 395 400 Ile Glu Asn Met Asn Gln Met Ala Asn Ala Ile Asp Thr Ser Ile Phe 405 410 415 Val Lys Asn Gly Pro Cys Ile Ala Gly Leu Gly Leu Gly Gly Glu Gly 420 425 430 Trp Thr Thr Met Thr Ile Thr Thr Pro Thr Gly Glu Gly Val Thr Ser 435 440 445 Ala Arg Thr Phe Val Arg Leu Arg Arg Cys Val Leu Val Asp Ala Phe 450 455 460 Arg Ile Val 465 <210> 98 <211> 469 <212> PRT <213> LIN1129li <400> 98 Met Glu Ser Leu Glu Leu Glu Gln Leu Val Lys Lys Val Leu Leu Glu 1 5 10 15 Lys Leu Ala Glu Gln Lys Glu Val Pro Thr Lys Thr Thr Thr Gln Gly 20 25 30 Ala Lys Ser Gly Val Phe Asp Thr Val Asp Glu Ala Val Gln Ala Ala 35 40 45 Val Ile Ala Gln Asn Cys Tyr Lys Glu Lys Ser Leu Glu Glu Arg Arg 50 55 60 Asn Val Val Lys Ala Ile Arg Glu Ala Leu Tyr Pro Glu Ile Glu Thr 65 70 75 80 Ile Ala Thr Arg Ala Val Ala Glu Thr Gly Met Gly Asn Val Thr Asp 85 90 95 Lys Ile Leu Lys Asn Thr Leu Ala Ile Glu Lys Thr Pro Gly Val Glu 100 105 110 Asp Leu Tyr Thr Glu Val Ala Thr Gly Asp Asn Gly Met Thr Leu Tyr 115 120 125 Glu Leu Ser Pro Tyr Gly Val Ile Gly Ala Val Ala Pro Ser Thr Asn 130 135 140 Pro Thr Glu Thr Leu Ile Cys Asn Ser Ile Gly Met Leu Ala Ala Gly 145 150 155 160 Asn Ala Val Phe Tyr Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu 165 170 175 Trp Leu Ile Glu Lys Leu Asn Thr Ile Val Arg Asp Ser Cys Gly Ile 180 185 190 Asp Asn Leu Ile Val Thr Val Ala Lys Pro Ser Ile Gln Ala Ala Gln 195 200 205 Glu Met Met Asn His Pro Lys Val Pro Leu Leu Val Ile Thr Gly Gly 210 215 220 Pro Gly Val Val Leu Gln Ala Met Gln Ser Gly Lys Lys Val Ile Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ser Ile Val Asp Glu Thr Ala Asn Ile 245 250 255 Glu Lys Ala Ala Ala Asp Ile Val Asp Gly Ala Ser Phe Asp His Asn 260 265 270 Ile Leu Cys Ile Ala Glu Lys Ser Val Val Ala Val Asp Ser Ile Ala 275 280 285 Asp Phe Leu Leu Phe Gln Met Glu Lys Asn Gly Ala Leu His Val Thr 290 295 300 Asn Pro Ser Asp Ile Gln Lys Leu Glu Lys Val Ala Val Thr Asp Lys 305 310 315 320 Gly Val Thr Asn Lys Lys Leu Val Gly Lys Ser Ala Thr Glu Ile Leu 325 330 335 Lys Glu Ala Gly Ile Ala Cys Asp Phe Thr Pro Arg Leu Ile Ile Val 340 345 350 Glu Thr Glu Lys Ser His Pro Phe Ala Thr Val Glu Leu Leu Met Pro 355 360 365 Ile Val Pro Val Val Arg Val Pro Asp Phe Asp Glu Ala Leu Glu Val 370 375 380 Ala Ile Glu Leu Glu Gln Gly Leu His His Thr Ala Thr Met His Ser 385 390 395 400 Gln Asn Ile Ser Arg Leu Asn Lys Ala Ala Arg Asp Met Gln Thr Ser 405 410 415 Ile Phe Val Lys Asn Gly Pro Ser Phe Ala Gly Leu Gly Phe Arg Gly 420 425 430 Glu Gly Ser Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr 435 440 445 Thr Thr Ala Arg His Phe Ala Arg Arg Arg Arg Cys Val Leu Thr Asp 450 455 460 Gly Phe Ser Ile Arg 465 <210> 99 <211> 2233 <212> PRT <213> ACC1sc_S659A <400> 99 Met Ser Glu Glu Ser Leu Phe Glu Ser Ser Pro Gln Lys Met Glu Tyr 1 5 10 15 Glu Ile Thr Asn Tyr Ser Glu Arg His Thr Glu Leu Pro Gly His Phe 20 25 30 Ile Gly Leu Asn Thr Val Asp Lys Leu Glu Glu Ser Pro Leu Arg Asp 35 40 45 Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile 50 55 60 Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys 65 70 75 80 Trp Ala Tyr Glu Thr Phe Gly Asp Asp Arg Thr Val Gln Phe Val Ala 85 90 95 Met Ala Thr Pro Glu Asp Leu Glu Ala Asn Ala Glu Tyr Ile Arg Met 100 105 110 Ala Asp Gln Tyr Ile Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr 115 120 125 Ala Asn Val Asp Leu Ile Val Asp Ile Ala Glu Arg Ala Asp Val Asp 130 135 140 Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro Leu Leu Pro 145 150 155 160 Glu Lys Leu Ser Gln Ser Lys Arg Lys Val Ile Phe Ile Gly Pro Pro 165 170 175 Gly Asn Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val 180 185 190 Ala Gln Ser Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val 195 200 205 Asp Thr Val His Val Asp Glu Lys Thr Gly Leu Val Ser Val Asp Asp 210 215 220 Asp Ile Tyr Gln Lys Gly Cys Cys Thr Ser Pro Glu Asp Gly Leu Gln 225 230 235 240 Lys Ala Lys Arg Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly 245 250 255 Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile 260 265 270 Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe 275 280 285 Ile Met Lys Leu Ala Gly Arg Ala Arg His Leu Glu Val Gln Leu Leu 290 295 300 Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser 305 310 315 320 Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile 325 330 335 Ala Lys Ala Glu Thr Phe His Glu Met Glu Lys Ala Ala Val Arg Leu 340 345 350 Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr 355 360 365 Ser His Asp Asp Gly Lys Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu 370 375 380 Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro 385 390 395 400 Ala Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser 405 410 415 Asp Ile Arg Thr Leu Tyr Gly Met Asn Pro His Ser Ala Ser Glu Ile 420 425 430 Asp Phe Glu Phe Lys Thr Gln Asp Ala Thr Lys Lys Gln Arg Arg Pro 435 440 445 Ile Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro 450 455 460 Asn Asp Gly Phe Lys Pro Ser Gly Gly Thr Leu His Glu Leu Asn Phe 465 470 475 480 Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly 485 490 495 Asn Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe 500 505 510 Gly Glu Asn Arg Gln Ala Ser Arg Lys His Met Val Val Ala Leu Lys 515 520 525 Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile 530 535 540 Lys Leu Leu Glu Thr Glu Asp Phe Glu Asp Asn Thr Ile Thr Thr Gly 545 550 555 560 Trp Leu Asp Asp Leu Ile Thr His Lys Met Thr Ala Glu Lys Pro Asp 565 570 575 Pro Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Leu Ala 580 585 590 Ser Glu Glu Ala Arg His Lys Tyr Ile Glu Ser Leu Gln Lys Gly Gln 595 600 605 Val Leu Ser Lys Asp Leu Leu Gln Thr Met Phe Pro Val Asp Phe Ile 610 615 620 His Glu Gly Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Gly Asn Asp 625 630 635 640 Arg Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Asp Ile Ile Leu Arg 645 650 655 Gln Leu Ala Asp Gly Gly Leu Leu Ile Ala Ile Gly Gly Lys Ser His 660 665 670 Thr Ile Tyr Trp Lys Glu Glu Val Ala Ala Thr Arg Leu Ser Val Asp 675 680 685 Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg 690 695 700 Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Glu 705 710 715 720 His Ile Ile Lys Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met 725 730 735 Gln Met Pro Leu Val Ser Gln Glu Asn Gly Ile Val Gln Leu Leu Lys 740 745 750 Gln Pro Gly Ser Thr Ile Val Ala Gly Asp Ile Met Ala Ile Met Thr 755 760 765 Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met 770 775 780 Leu Pro Asp Phe Gly Ser Pro Val Ile Glu Gly Thr Lys Pro Ala Tyr 785 790 795 800 Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr 805 810 815 Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val 820 825 830 Leu Arg Asn Pro Lys Leu Pro Tyr Ser Glu Trp Lys Leu His Ile Ser 835 840 845 Ala Leu His Ser Arg Leu Pro Ala Lys Leu Asp Glu Gln Met Glu Glu 850 855 860 Leu Val Ala Arg Ser Leu Arg Arg Gly Ala Val Phe Pro Ala Arg Gln 865 870 875 880 Leu Ser Lys Leu Ile Asp Met Ala Val Lys Asn Pro Glu Tyr Asn Pro 885 890 895 Asp Lys Leu Leu Gly Ala Val Val Glu Pro Leu Ala Asp Ile Ala His 900 905 910 Lys Tyr Ser Asn Gly Leu Glu Ala His Glu His Ser Ile Phe Val His 915 920 925 Phe Leu Glu Glu Tyr Tyr Glu Val Glu Lys Leu Phe Asn Gly Pro Asn 930 935 940 Val Arg Glu Glu Asn Ile Ile Leu Lys Leu Arg Asp Glu Asn Pro Lys 945 950 955 960 Asp Leu Asp Lys Val Ala Leu Thr Val Leu Ser His Ser Lys Val Ser 965 970 975 Ala Lys Asn Asn Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Leu 980 985 990 Cys Lys Leu Ser Ser Lys Val Ser Ala Ile Phe Ser Thr Pro Leu Gln 995 1000 1005 His Ile Val Glu Leu Glu Ser Lys Ala Thr Ala Lys Val Ala Leu Gln 1010 1015 1020 Ala Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg 1025 1030 1035 1040 Thr Glu Gln Ile Glu His Ile Leu Lys Ser Ser Val Val Lys Val Ala 1045 1050 1055 Tyr Gly Ser Ser Asn Pro Lys Arg Ser Glu Pro Asp Leu Asn Ile Leu 1060 1065 1070 Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val Leu Leu Gln 1075 1080 1085 Phe Leu Thr His Gln Asp Pro Val Val Thr Ala Ala Ala Ala Gln Val 1090 1095 1100 Tyr Ile Arg Arg Ala Tyr Arg Ala Tyr Thr Ile Gly Asp Ile Arg Val 1105 1110 1115 1120 His Glu Gly Val Thr Val Pro Ile Val Glu Trp Lys Phe Gln Leu Pro 1125 1130 1135 Ser Ala Ala Phe Ser Thr Phe Pro Thr Val Lys Ser Lys Met Gly Met 1140 1145 1150 Asn Arg Ala Val Ser Val Ser Asp Leu Ser Tyr Val Ala Asn Ser Gln 1155 1160 1165 Ser Ser Pro Leu Arg Glu Gly Ile Leu Met Ala Val Asp His Leu Asp 1170 1175 1180 Asp Val Asp Glu Ile Leu Ser Gln Ser Leu Glu Val Ile Pro Arg His 1185 1190 1195 1200 Gln Ser Ser Ser Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ala 1205 1210 1215 Ser Leu Ser Asn Val Ala Asn Val Cys Val Ala Ser Thr Glu Gly Phe 1220 1225 1230 Glu Ser Glu Glu Glu Ile Leu Val Arg Leu Arg Glu Ile Leu Asp Leu 1235 1240 1245 Asn Lys Gln Glu Leu Ile Asn Ala Ser Ile Arg Arg Ile Thr Phe Met 1250 1255 1260 Phe Gly Phe Lys Asp Gly Ser Tyr Pro Lys Tyr Tyr Thr Phe Asn Gly 1265 1270 1275 1280 Pro Asn Tyr Asn Glu Asn Glu Thr Ile Arg His Ile Glu Pro Ala Leu 1285 1290 1295 Ala Phe Gln Leu Glu Leu Gly Arg Leu Ser Asn Phe Asn Ile Lys Pro 1300 1305 1310 Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr Glu Ala Val Ser Lys 1315 1320 1325 Thr Ser Pro Leu Asp Lys Arg Phe Phe Thr Arg Gly Ile Ile Arg Thr 1330 1335 1340 Gly His Ile Arg Asp Asp Ile Ser Ile Gln Glu Tyr Leu Thr Ser Glu 1345 1350 1355 1360 Ala Asn Arg Leu Met Ser Asp Ile Leu Asp Asn Leu Glu Val Thr Asp 1365 1370 1375 Thr Ser Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ile Ala Val 1380 1385 1390 Phe Asp Ile Ser Pro Glu Asp Val Glu Ala Ala Phe Gly Gly Phe Leu 1395 1400 1405 Glu Arg Phe Gly Lys Arg Leu Leu Arg Leu Arg Val Ser Ser Ala Glu 1410 1415 1420 Ile Arg Ile Ile Ile Lys Asp Pro Gln Thr Gly Ala Pro Val Pro Leu 1425 1430 1435 1440 Arg Ala Leu Ile Asn Asn Val Ser Gly Tyr Val Ile Lys Thr Glu Met 1445 1450 1455 Tyr Thr Glu Val Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu 1460 1465 1470 Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr Pro 1475 1480 1485 Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met Gly 1490 1495 1500 Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg Gln Ala Ser Ser 1505 1510 1515 1520 Ser Gln Trp Lys Asn Phe Ser Ala Asp Val Lys Leu Thr Asp Asp Phe 1525 1530 1535 Phe Ile Ser Asn Glu Leu Ile Glu Asp Glu Asn Gly Glu Leu Thr Glu 1540 1545 1550 Val Glu Arg Glu Pro Gly Ala Asn Ala Ile Gly Met Val Ala Phe Lys 1555 1560 1565 Ile Thr Val Lys Thr Pro Glu Tyr Pro Arg Gly Arg Gln Phe Val Val 1570 1575 1580 Val Ala Asn Asp Ile Thr Phe Lys Ile Gly Ser Phe Gly Pro Gln Glu 1585 1590 1595 1600 Asp Glu Phe Phe Asn Lys Val Thr Glu Tyr Ala Arg Lys Arg Gly Ile 1605 1610 1615 Pro Arg Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg Ile Gly Met Ala 1620 1625 1630 Glu Glu Ile Val Pro Leu Phe Gln Val Ala Trp Asn Asp Ala Ala Asn 1635 1640 1645 Pro Asp Lys Gly Phe Gln Tyr Leu Tyr Leu Thr Ser Glu Gly Met Glu 1650 1655 1660 Thr Leu Lys Lys Phe Asp Lys Glu Asn Ser Val Leu Thr Glu Arg Thr 1665 1670 1675 1680 Val Ile Asn Gly Glu Glu Arg Phe Val Ile Lys Thr Ile Ile Gly Ser 1685 1690 1695 Glu Asp Gly Leu Gly Val Glu Cys Leu Arg Gly Ser Gly Leu Ile Ala 1700 1705 1710 Gly Ala Thr Ser Arg Ala Tyr His Asp Ile Phe Thr Ile Thr Leu Val 1715 1720 1725 Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val Arg Leu Gly Gln 1730 1735 1740 Arg Ala Ile Gln Val Glu Gly Gln Pro Ile Ile Leu Thr Gly Ala Pro 1745 1750 1755 1760 Ala Ile Asn Lys Met Leu Gly Arg Glu Val Tyr Thr Ser Asn Leu Gln 1765 1770 1775 Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val Ser His Leu Thr 1780 1785 1790 Ala Val Asp Asp Leu Ala Gly Val Glu Lys Ile Val Glu Trp Met Ser 1795 1800 1805 Tyr Val Pro Ala Lys Arg Asn Met Pro Val Pro Ile Leu Glu Thr Lys 1810 1815 1820 Asp Thr Trp Asp Arg Pro Val Asp Phe Thr Pro Thr Asn Asp Glu Thr 1825 1830 1835 1840 Tyr Asp Val Arg Trp Met Ile Glu Gly Arg Glu Thr Glu Ser Gly Phe 1845 1850 1855 Glu Tyr Gly Leu Phe Asp Lys Gly Ser Phe Phe Glu Thr Leu Ser Gly 1860 1865 1870 Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg Leu Gly Gly Ile Pro 1875 1880 1885 Leu Gly Val Ile Gly Val Glu Thr Arg Thr Val Glu Asn Leu Ile Pro 1890 1895 1900 Ala Asp Pro Ala Asn Pro Asn Ser Ala Glu Thr Leu Ile Gln Glu Pro 1905 1910 1915 1920 Gly Gln Val Trp His Pro Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile 1925 1930 1935 Asn Asp Phe Asn Asn Gly Glu Gln Leu Pro Met Met Ile Leu Ala Asn 1940 1945 1950 Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Phe Asn Glu Val Leu 1955 1960 1965 Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu Val Asp Tyr Lys Gln Pro 1970 1975 1980 Ile Ile Ile Tyr Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp 1985 1990 1995 2000 Val Val Val Asp Pro Thr Ile Asn Ala Asp Gln Met Glu Met Tyr Ala 2005 2010 2015 Asp Val Asn Ala Arg Ala Gly Val Leu Glu Pro Gln Gly Met Val Gly 2020 2025 2030 Ile Lys Phe Arg Arg Glu Lys Leu Leu Asp Thr Met Asn Arg Leu Asp 2035 2040 2045 Asp Lys Tyr Arg Glu Leu Arg Ser Gln Leu Ser Asn Lys Ser Leu Ala 2050 2055 2060 Pro Glu Val His Gln Gln Ile Ser Lys Gln Leu Ala Asp Arg Glu Arg 2065 2070 2075 2080 Glu Leu Leu Pro Ile Tyr Gly Gln Ile Ser Leu Gln Phe Ala Asp Leu 2085 2090 2095 His Asp Arg Ser Ser Arg Met Val Ala Lys Gly Val Ile Ser Lys Glu 2100 2105 2110 Leu Glu Trp Thr Glu Ala Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg 2115 2120 2125 Arg Leu Asn Glu Glu Tyr Leu Ile Lys Arg Leu Ser His Gln Val Gly 2130 2135 2140 Glu Ala Ser Arg Leu Glu Lys Ile Ala Arg Ile Arg Ser Trp Tyr Pro 2145 2150 2155 2160 Ala Ser Val Asp His Glu Asp Asp Arg Gln Val Ala Thr Trp Ile Glu 2165 2170 2175 Glu Asn Tyr Lys Thr Leu Asp Asp Lys Leu Lys Gly Leu Lys Leu Glu 2180 2185 2190 Ser Phe Ala Gln Asp Leu Ala Lys Lys Ile Arg Ser Asp His Asp Asn 2195 2200 2205 Ala Ile Asp Gly Leu Ser Glu Val Ile Lys Met Leu Ser Thr Asp Asp 2210 2215 2220 Lys Glu Lys Leu Leu Lys Thr Leu Lys 2225 2230 <210> 100 <211> 2233 <212> PRT <213> ACC1sc_S659A/S1157A <400> 100 Met Ser Glu Glu Ser Leu Phe Glu Ser Ser Pro Gln Lys Met Glu Tyr 1 5 10 15 Glu Ile Thr Asn Tyr Ser Glu Arg His Thr Glu Leu Pro Gly His Phe 20 25 30 Ile Gly Leu Asn Thr Val Asp Lys Leu Glu Glu Ser Pro Leu Arg Asp 35 40 45 Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile 50 55 60 Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys 65 70 75 80 Trp Ala Tyr Glu Thr Phe Gly Asp Asp Arg Thr Val Gln Phe Val Ala 85 90 95 Met Ala Thr Pro Glu Asp Leu Glu Ala Asn Ala Glu Tyr Ile Arg Met 100 105 110 Ala Asp Gln Tyr Ile Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr 115 120 125 Ala Asn Val Asp Leu Ile Val Asp Ile Ala Glu Arg Ala Asp Val Asp 130 135 140 Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro Leu Leu Pro 145 150 155 160 Glu Lys Leu Ser Gln Ser Lys Arg Lys Val Ile Phe Ile Gly Pro Pro 165 170 175 Gly Asn Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val 180 185 190 Ala Gln Ser Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val 195 200 205 Asp Thr Val His Val Asp Glu Lys Thr Gly Leu Val Ser Val Asp Asp 210 215 220 Asp Ile Tyr Gln Lys Gly Cys Cys Thr Ser Pro Glu Asp Gly Leu Gln 225 230 235 240 Lys Ala Lys Arg Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly 245 250 255 Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile 260 265 270 Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe 275 280 285 Ile Met Lys Leu Ala Gly Arg Ala Arg His Leu Glu Val Gln Leu Leu 290 295 300 Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser 305 310 315 320 Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile 325 330 335 Ala Lys Ala Glu Thr Phe His Glu Met Glu Lys Ala Ala Val Arg Leu 340 345 350 Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr 355 360 365 Ser His Asp Asp Gly Lys Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu 370 375 380 Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro 385 390 395 400 Ala Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser 405 410 415 Asp Ile Arg Thr Leu Tyr Gly Met Asn Pro His Ser Ala Ser Glu Ile 420 425 430 Asp Phe Glu Phe Lys Thr Gln Asp Ala Thr Lys Lys Gln Arg Arg Pro 435 440 445 Ile Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro 450 455 460 Asn Asp Gly Phe Lys Pro Ser Gly Gly Thr Leu His Glu Leu Asn Phe 465 470 475 480 Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly 485 490 495 Asn Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe 500 505 510 Gly Glu Asn Arg Gln Ala Ser Arg Lys His Met Val Val Ala Leu Lys 515 520 525 Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile 530 535 540 Lys Leu Leu Glu Thr Glu Asp Phe Glu Asp Asn Thr Ile Thr Thr Gly 545 550 555 560 Trp Leu Asp Asp Leu Ile Thr His Lys Met Thr Ala Glu Lys Pro Asp 565 570 575 Pro Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Leu Ala 580 585 590 Ser Glu Glu Ala Arg His Lys Tyr Ile Glu Ser Leu Gln Lys Gly Gln 595 600 605 Val Leu Ser Lys Asp Leu Leu Gln Thr Met Phe Pro Val Asp Phe Ile 610 615 620 His Glu Gly Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Gly Asn Asp 625 630 635 640 Arg Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Asp Ile Ile Leu Arg 645 650 655 Gln Leu Ala Asp Gly Gly Leu Leu Ile Ala Ile Gly Gly Lys Ser His 660 665 670 Thr Ile Tyr Trp Lys Glu Glu Val Ala Ala Thr Arg Leu Ser Val Asp 675 680 685 Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg 690 695 700 Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Glu 705 710 715 720 His Ile Ile Lys Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met 725 730 735 Gln Met Pro Leu Val Ser Gln Glu Asn Gly Ile Val Gln Leu Leu Lys 740 745 750 Gln Pro Gly Ser Thr Ile Val Ala Gly Asp Ile Met Ala Ile Met Thr 755 760 765 Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met 770 775 780 Leu Pro Asp Phe Gly Ser Pro Val Ile Glu Gly Thr Lys Pro Ala Tyr 785 790 795 800 Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr 805 810 815 Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val 820 825 830 Leu Arg Asn Pro Lys Leu Pro Tyr Ser Glu Trp Lys Leu His Ile Ser 835 840 845 Ala Leu His Ser Arg Leu Pro Ala Lys Leu Asp Glu Gln Met Glu Glu 850 855 860 Leu Val Ala Arg Ser Leu Arg Arg Gly Ala Val Phe Pro Ala Arg Gln 865 870 875 880 Leu Ser Lys Leu Ile Asp Met Ala Val Lys Asn Pro Glu Tyr Asn Pro 885 890 895 Asp Lys Leu Leu Gly Ala Val Val Glu Pro Leu Ala Asp Ile Ala His 900 905 910 Lys Tyr Ser Asn Gly Leu Glu Ala His Glu His Ser Ile Phe Val His 915 920 925 Phe Leu Glu Glu Tyr Tyr Glu Val Glu Lys Leu Phe Asn Gly Pro Asn 930 935 940 Val Arg Glu Glu Asn Ile Ile Leu Lys Leu Arg Asp Glu Asn Pro Lys 945 950 955 960 Asp Leu Asp Lys Val Ala Leu Thr Val Leu Ser His Ser Lys Val Ser 965 970 975 Ala Lys Asn Asn Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Leu 980 985 990 Cys Lys Leu Ser Ser Lys Val Ser Ala Ile Phe Ser Thr Pro Leu Gln 995 1000 1005 His Ile Val Glu Leu Glu Ser Lys Ala Thr Ala Lys Val Ala Leu Gln 1010 1015 1020 Ala Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg 1025 1030 1035 1040 Thr Glu Gln Ile Glu His Ile Leu Lys Ser Ser Val Val Lys Val Ala 1045 1050 1055 Tyr Gly Ser Ser Asn Pro Lys Arg Ser Glu Pro Asp Leu Asn Ile Leu 1060 1065 1070 Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val Leu Leu Gln 1075 1080 1085 Phe Leu Thr His Gln Asp Pro Val Val Thr Ala Ala Ala Ala Gln Val 1090 1095 1100 Tyr Ile Arg Arg Ala Tyr Arg Ala Tyr Thr Ile Gly Asp Ile Arg Val 1105 1110 1115 1120 His Glu Gly Val Thr Val Pro Ile Val Glu Trp Lys Phe Gln Leu Pro 1125 1130 1135 Ser Ala Ala Phe Ser Thr Phe Pro Thr Val Lys Ser Lys Met Gly Met 1140 1145 1150 Asn Arg Ala Val Ala Val Ser Asp Leu Ser Tyr Val Ala Asn Ser Gln 1155 1160 1165 Ser Ser Pro Leu Arg Glu Gly Ile Leu Met Ala Val Asp His Leu Asp 1170 1175 1180 Asp Val Asp Glu Ile Leu Ser Gln Ser Leu Glu Val Ile Pro Arg His 1185 1190 1195 1200 Gln Ser Ser Ser Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ala 1205 1210 1215 Ser Leu Ser Asn Val Ala Asn Val Cys Val Ala Ser Thr Glu Gly Phe 1220 1225 1230 Glu Ser Glu Glu Glu Ile Leu Val Arg Leu Arg Glu Ile Leu Asp Leu 1235 1240 1245 Asn Lys Gln Glu Leu Ile Asn Ala Ser Ile Arg Arg Ile Thr Phe Met 1250 1255 1260 Phe Gly Phe Lys Asp Gly Ser Tyr Pro Lys Tyr Tyr Thr Phe Asn Gly 1265 1270 1275 1280 Pro Asn Tyr Asn Glu Asn Glu Thr Ile Arg His Ile Glu Pro Ala Leu 1285 1290 1295 Ala Phe Gln Leu Glu Leu Gly Arg Leu Ser Asn Phe Asn Ile Lys Pro 1300 1305 1310 Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr Glu Ala Val Ser Lys 1315 1320 1325 Thr Ser Pro Leu Asp Lys Arg Phe Phe Thr Arg Gly Ile Ile Arg Thr 1330 1335 1340 Gly His Ile Arg Asp Asp Ile Ser Ile Gln Glu Tyr Leu Thr Ser Glu 1345 1350 1355 1360 Ala Asn Arg Leu Met Ser Asp Ile Leu Asp Asn Leu Glu Val Thr Asp 1365 1370 1375 Thr Ser Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ile Ala Val 1380 1385 1390 Phe Asp Ile Ser Pro Glu Asp Val Glu Ala Ala Phe Gly Gly Phe Leu 1395 1400 1405 Glu Arg Phe Gly Lys Arg Leu Leu Arg Leu Arg Val Ser Ser Ala Glu 1410 1415 1420 Ile Arg Ile Ile Ile Lys Asp Pro Gln Thr Gly Ala Pro Val Pro Leu 1425 1430 1435 1440 Arg Ala Leu Ile Asn Asn Val Ser Gly Tyr Val Ile Lys Thr Glu Met 1445 1450 1455 Tyr Thr Glu Val Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu 1460 1465 1470 Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr Pro 1475 1480 1485 Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met Gly 1490 1495 1500 Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg Gln Ala Ser Ser 1505 1510 1515 1520 Ser Gln Trp Lys Asn Phe Ser Ala Asp Val Lys Leu Thr Asp Asp Phe 1525 1530 1535 Phe Ile Ser Asn Glu Leu Ile Glu Asp Glu Asn Gly Glu Leu Thr Glu 1540 1545 1550 Val Glu Arg Glu Pro Gly Ala Asn Ala Ile Gly Met Val Ala Phe Lys 1555 1560 1565 Ile Thr Val Lys Thr Pro Glu Tyr Pro Arg Gly Arg Gln Phe Val Val 1570 1575 1580 Val Ala Asn Asp Ile Thr Phe Lys Ile Gly Ser Phe Gly Pro Gln Glu 1585 1590 1595 1600 Asp Glu Phe Phe Asn Lys Val Thr Glu Tyr Ala Arg Lys Arg Gly Ile 1605 1610 1615 Pro Arg Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg Ile Gly Met Ala 1620 1625 1630 Glu Glu Ile Val Pro Leu Phe Gln Val Ala Trp Asn Asp Ala Ala Asn 1635 1640 1645 Pro Asp Lys Gly Phe Gln Tyr Leu Tyr Leu Thr Ser Glu Gly Met Glu 1650 1655 1660 Thr Leu Lys Lys Phe Asp Lys Glu Asn Ser Val Leu Thr Glu Arg Thr 1665 1670 1675 1680 Val Ile Asn Gly Glu Glu Arg Phe Val Ile Lys Thr Ile Ile Gly Ser 1685 1690 1695 Glu Asp Gly Leu Gly Val Glu Cys Leu Arg Gly Ser Gly Leu Ile Ala 1700 1705 1710 Gly Ala Thr Ser Arg Ala Tyr His Asp Ile Phe Thr Ile Thr Leu Val 1715 1720 1725 Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val Arg Leu Gly Gln 1730 1735 1740 Arg Ala Ile Gln Val Glu Gly Gln Pro Ile Ile Leu Thr Gly Ala Pro 1745 1750 1755 1760 Ala Ile Asn Lys Met Leu Gly Arg Glu Val Tyr Thr Ser Asn Leu Gln 1765 1770 1775 Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val Ser His Leu Thr 1780 1785 1790 Ala Val Asp Asp Leu Ala Gly Val Glu Lys Ile Val Glu Trp Met Ser 1795 1800 1805 Tyr Val Pro Ala Lys Arg Asn Met Pro Val Pro Ile Leu Glu Thr Lys 1810 1815 1820 Asp Thr Trp Asp Arg Pro Val Asp Phe Thr Pro Thr Asn Asp Glu Thr 1825 1830 1835 1840 Tyr Asp Val Arg Trp Met Ile Glu Gly Arg Glu Thr Glu Ser Gly Phe 1845 1850 1855 Glu Tyr Gly Leu Phe Asp Lys Gly Ser Phe Phe Glu Thr Leu Ser Gly 1860 1865 1870 Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg Leu Gly Gly Ile Pro 1875 1880 1885 Leu Gly Val Ile Gly Val Glu Thr Arg Thr Val Glu Asn Leu Ile Pro 1890 1895 1900 Ala Asp Pro Ala Asn Pro Asn Ser Ala Glu Thr Leu Ile Gln Glu Pro 1905 1910 1915 1920 Gly Gln Val Trp His Pro Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile 1925 1930 1935 Asn Asp Phe Asn Asn Gly Glu Gln Leu Pro Met Met Ile Leu Ala Asn 1940 1945 1950 Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Phe Asn Glu Val Leu 1955 1960 1965 Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu Val Asp Tyr Lys Gln Pro 1970 1975 1980 Ile Ile Ile Tyr Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp 1985 1990 1995 2000 Val Val Val Asp Pro Thr Ile Asn Ala Asp Gln Met Glu Met Tyr Ala 2005 2010 2015 Asp Val Asn Ala Arg Ala Gly Val Leu Glu Pro Gln Gly Met Val Gly 2020 2025 2030 Ile Lys Phe Arg Arg Glu Lys Leu Leu Asp Thr Met Asn Arg Leu Asp 2035 2040 2045 Asp Lys Tyr Arg Glu Leu Arg Ser Gln Leu Ser Asn Lys Ser Leu Ala 2050 2055 2060 Pro Glu Val His Gln Gln Ile Ser Lys Gln Leu Ala Asp Arg Glu Arg 2065 2070 2075 2080 Glu Leu Leu Pro Ile Tyr Gly Gln Ile Ser Leu Gln Phe Ala Asp Leu 2085 2090 2095 His Asp Arg Ser Ser Arg Met Val Ala Lys Gly Val Ile Ser Lys Glu 2100 2105 2110 Leu Glu Trp Thr Glu Ala Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg 2115 2120 2125 Arg Leu Asn Glu Glu Tyr Leu Ile Lys Arg Leu Ser His Gln Val Gly 2130 2135 2140 Glu Ala Ser Arg Leu Glu Lys Ile Ala Arg Ile Arg Ser Trp Tyr Pro 2145 2150 2155 2160 Ala Ser Val Asp His Glu Asp Asp Arg Gln Val Ala Thr Trp Ile Glu 2165 2170 2175 Glu Asn Tyr Lys Thr Leu Asp Asp Lys Leu Lys Gly Leu Lys Leu Glu 2180 2185 2190 Ser Phe Ala Gln Asp Leu Ala Lys Lys Ile Arg Ser Asp His Asp Asn 2195 2200 2205 Ala Ile Asp Gly Leu Ser Glu Val Ile Lys Met Leu Ser Thr Asp Asp 2210 2215 2220 Lys Glu Lys Leu Leu Lys Thr Leu Lys 2225 2230 <210> 101 <211> 2233 <212> PRT <213> ACC1sc_S1157A <400> 101 Met Ser Glu Glu Ser Leu Phe Glu Ser Ser Pro Gln Lys Met Glu Tyr 1 5 10 15 Glu Ile Thr Asn Tyr Ser Glu Arg His Thr Glu Leu Pro Gly His Phe 20 25 30 Ile Gly Leu Asn Thr Val Asp Lys Leu Glu Glu Ser Pro Leu Arg Asp 35 40 45 Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile 50 55 60 Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys 65 70 75 80 Trp Ala Tyr Glu Thr Phe Gly Asp Asp Arg Thr Val Gln Phe Val Ala 85 90 95 Met Ala Thr Pro Glu Asp Leu Glu Ala Asn Ala Glu Tyr Ile Arg Met 100 105 110 Ala Asp Gln Tyr Ile Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr 115 120 125 Ala Asn Val Asp Leu Ile Val Asp Ile Ala Glu Arg Ala Asp Val Asp 130 135 140 Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro Leu Leu Pro 145 150 155 160 Glu Lys Leu Ser Gln Ser Lys Arg Lys Val Ile Phe Ile Gly Pro Pro 165 170 175 Gly Asn Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val 180 185 190 Ala Gln Ser Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val 195 200 205 Asp Thr Val His Val Asp Glu Lys Thr Gly Leu Val Ser Val Asp Asp 210 215 220 Asp Ile Tyr Gln Lys Gly Cys Cys Thr Ser Pro Glu Asp Gly Leu Gln 225 230 235 240 Lys Ala Lys Arg Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly 245 250 255 Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile 260 265 270 Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe 275 280 285 Ile Met Lys Leu Ala Gly Arg Ala Arg His Leu Glu Val Gln Leu Leu 290 295 300 Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser 305 310 315 320 Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile 325 330 335 Ala Lys Ala Glu Thr Phe His Glu Met Glu Lys Ala Ala Val Arg Leu 340 345 350 Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr 355 360 365 Ser His Asp Asp Gly Lys Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu 370 375 380 Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro 385 390 395 400 Ala Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser 405 410 415 Asp Ile Arg Thr Leu Tyr Gly Met Asn Pro His Ser Ala Ser Glu Ile 420 425 430 Asp Phe Glu Phe Lys Thr Gln Asp Ala Thr Lys Lys Gln Arg Arg Pro 435 440 445 Ile Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro 450 455 460 Asn Asp Gly Phe Lys Pro Ser Gly Gly Thr Leu His Glu Leu Asn Phe 465 470 475 480 Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly 485 490 495 Asn Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe 500 505 510 Gly Glu Asn Arg Gln Ala Ser Arg Lys His Met Val Val Ala Leu Lys 515 520 525 Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile 530 535 540 Lys Leu Leu Glu Thr Glu Asp Phe Glu Asp Asn Thr Ile Thr Thr Gly 545 550 555 560 Trp Leu Asp Asp Leu Ile Thr His Lys Met Thr Ala Glu Lys Pro Asp 565 570 575 Pro Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Leu Ala 580 585 590 Ser Glu Glu Ala Arg His Lys Tyr Ile Glu Ser Leu Gln Lys Gly Gln 595 600 605 Val Leu Ser Lys Asp Leu Leu Gln Thr Met Phe Pro Val Asp Phe Ile 610 615 620 His Glu Gly Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Gly Asn Asp 625 630 635 640 Arg Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Asp Ile Ile Leu Arg 645 650 655 Gln Leu Ser Asp Gly Gly Leu Leu Ile Ala Ile Gly Gly Lys Ser His 660 665 670 Thr Ile Tyr Trp Lys Glu Glu Val Ala Ala Thr Arg Leu Ser Val Asp 675 680 685 Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg 690 695 700 Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Glu 705 710 715 720 His Ile Ile Lys Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met 725 730 735 Gln Met Pro Leu Val Ser Gln Glu Asn Gly Ile Val Gln Leu Leu Lys 740 745 750 Gln Pro Gly Ser Thr Ile Val Ala Gly Asp Ile Met Ala Ile Met Thr 755 760 765 Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met 770 775 780 Leu Pro Asp Phe Gly Ser Pro Val Ile Glu Gly Thr Lys Pro Ala Tyr 785 790 795 800 Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr 805 810 815 Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val 820 825 830 Leu Arg Asn Pro Lys Leu Pro Tyr Ser Glu Trp Lys Leu His Ile Ser 835 840 845 Ala Leu His Ser Arg Leu Pro Ala Lys Leu Asp Glu Gln Met Glu Glu 850 855 860 Leu Val Ala Arg Ser Leu Arg Arg Gly Ala Val Phe Pro Ala Arg Gln 865 870 875 880 Leu Ser Lys Leu Ile Asp Met Ala Val Lys Asn Pro Glu Tyr Asn Pro 885 890 895 Asp Lys Leu Leu Gly Ala Val Val Glu Pro Leu Ala Asp Ile Ala His 900 905 910 Lys Tyr Ser Asn Gly Leu Glu Ala His Glu His Ser Ile Phe Val His 915 920 925 Phe Leu Glu Glu Tyr Tyr Glu Val Glu Lys Leu Phe Asn Gly Pro Asn 930 935 940 Val Arg Glu Glu Asn Ile Ile Leu Lys Leu Arg Asp Glu Asn Pro Lys 945 950 955 960 Asp Leu Asp Lys Val Ala Leu Thr Val Leu Ser His Ser Lys Val Ser 965 970 975 Ala Lys Asn Asn Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Leu 980 985 990 Cys Lys Leu Ser Ser Lys Val Ser Ala Ile Phe Ser Thr Pro Leu Gln 995 1000 1005 His Ile Val Glu Leu Glu Ser Lys Ala Thr Ala Lys Val Ala Leu Gln 1010 1015 1020 Ala Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg 1025 1030 1035 1040 Thr Glu Gln Ile Glu His Ile Leu Lys Ser Ser Val Val Lys Val Ala 1045 1050 1055 Tyr Gly Ser Ser Asn Pro Lys Arg Ser Glu Pro Asp Leu Asn Ile Leu 1060 1065 1070 Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val Leu Leu Gln 1075 1080 1085 Phe Leu Thr His Gln Asp Pro Val Val Thr Ala Ala Ala Ala Gln Val 1090 1095 1100 Tyr Ile Arg Arg Ala Tyr Arg Ala Tyr Thr Ile Gly Asp Ile Arg Val 1105 1110 1115 1120 His Glu Gly Val Thr Val Pro Ile Val Glu Trp Lys Phe Gln Leu Pro 1125 1130 1135 Ser Ala Ala Phe Ser Thr Phe Pro Thr Val Lys Ser Lys Met Gly Met 1140 1145 1150 Asn Arg Ala Val Ala Val Ser Asp Leu Ser Tyr Val Ala Asn Ser Gln 1155 1160 1165 Ser Ser Pro Leu Arg Glu Gly Ile Leu Met Ala Val Asp His Leu Asp 1170 1175 1180 Asp Val Asp Glu Ile Leu Ser Gln Ser Leu Glu Val Ile Pro Arg His 1185 1190 1195 1200 Gln Ser Ser Ser Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ala 1205 1210 1215 Ser Leu Ser Asn Val Ala Asn Val Cys Val Ala Ser Thr Glu Gly Phe 1220 1225 1230 Glu Ser Glu Glu Glu Ile Leu Val Arg Leu Arg Glu Ile Leu Asp Leu 1235 1240 1245 Asn Lys Gln Glu Leu Ile Asn Ala Ser Ile Arg Arg Ile Thr Phe Met 1250 1255 1260 Phe Gly Phe Lys Asp Gly Ser Tyr Pro Lys Tyr Tyr Thr Phe Asn Gly 1265 1270 1275 1280 Pro Asn Tyr Asn Glu Asn Glu Thr Ile Arg His Ile Glu Pro Ala Leu 1285 1290 1295 Ala Phe Gln Leu Glu Leu Gly Arg Leu Ser Asn Phe Asn Ile Lys Pro 1300 1305 1310 Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr Glu Ala Val Ser Lys 1315 1320 1325 Thr Ser Pro Leu Asp Lys Arg Phe Phe Thr Arg Gly Ile Ile Arg Thr 1330 1335 1340 Gly His Ile Arg Asp Asp Ile Ser Ile Gln Glu Tyr Leu Thr Ser Glu 1345 1350 1355 1360 Ala Asn Arg Leu Met Ser Asp Ile Leu Asp Asn Leu Glu Val Thr Asp 1365 1370 1375 Thr Ser Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ile Ala Val 1380 1385 1390 Phe Asp Ile Ser Pro Glu Asp Val Glu Ala Ala Phe Gly Gly Phe Leu 1395 1400 1405 Glu Arg Phe Gly Lys Arg Leu Leu Arg Leu Arg Val Ser Ser Ala Glu 1410 1415 1420 Ile Arg Ile Ile Ile Lys Asp Pro Gln Thr Gly Ala Pro Val Pro Leu 1425 1430 1435 1440 Arg Ala Leu Ile Asn Asn Val Ser Gly Tyr Val Ile Lys Thr Glu Met 1445 1450 1455 Tyr Thr Glu Val Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu 1460 1465 1470 Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr Pro 1475 1480 1485 Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met Gly 1490 1495 1500 Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg Gln Ala Ser Ser 1505 1510 1515 1520 Ser Gln Trp Lys Asn Phe Ser Ala Asp Val Lys Leu Thr Asp Asp Phe 1525 1530 1535 Phe Ile Ser Asn Glu Leu Ile Glu Asp Glu Asn Gly Glu Leu Thr Glu 1540 1545 1550 Val Glu Arg Glu Pro Gly Ala Asn Ala Ile Gly Met Val Ala Phe Lys 1555 1560 1565 Ile Thr Val Lys Thr Pro Glu Tyr Pro Arg Gly Arg Gln Phe Val Val 1570 1575 1580 Val Ala Asn Asp Ile Thr Phe Lys Ile Gly Ser Phe Gly Pro Gln Glu 1585 1590 1595 1600 Asp Glu Phe Phe Asn Lys Val Thr Glu Tyr Ala Arg Lys Arg Gly Ile 1605 1610 1615 Pro Arg Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg Ile Gly Met Ala 1620 1625 1630 Glu Glu Ile Val Pro Leu Phe Gln Val Ala Trp Asn Asp Ala Ala Asn 1635 1640 1645 Pro Asp Lys Gly Phe Gln Tyr Leu Tyr Leu Thr Ser Glu Gly Met Glu 1650 1655 1660 Thr Leu Lys Lys Phe Asp Lys Glu Asn Ser Val Leu Thr Glu Arg Thr 1665 1670 1675 1680 Val Ile Asn Gly Glu Glu Arg Phe Val Ile Lys Thr Ile Ile Gly Ser 1685 1690 1695 Glu Asp Gly Leu Gly Val Glu Cys Leu Arg Gly Ser Gly Leu Ile Ala 1700 1705 1710 Gly Ala Thr Ser Arg Ala Tyr His Asp Ile Phe Thr Ile Thr Leu Val 1715 1720 1725 Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val Arg Leu Gly Gln 1730 1735 1740 Arg Ala Ile Gln Val Glu Gly Gln Pro Ile Ile Leu Thr Gly Ala Pro 1745 1750 1755 1760 Ala Ile Asn Lys Met Leu Gly Arg Glu Val Tyr Thr Ser Asn Leu Gln 1765 1770 1775 Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val Ser His Leu Thr 1780 1785 1790 Ala Val Asp Asp Leu Ala Gly Val Glu Lys Ile Val Glu Trp Met Ser 1795 1800 1805 Tyr Val Pro Ala Lys Arg Asn Met Pro Val Pro Ile Leu Glu Thr Lys 1810 1815 1820 Asp Thr Trp Asp Arg Pro Val Asp Phe Thr Pro Thr Asn Asp Glu Thr 1825 1830 1835 1840 Tyr Asp Val Arg Trp Met Ile Glu Gly Arg Glu Thr Glu Ser Gly Phe 1845 1850 1855 Glu Tyr Gly Leu Phe Asp Lys Gly Ser Phe Phe Glu Thr Leu Ser Gly 1860 1865 1870 Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg Leu Gly Gly Ile Pro 1875 1880 1885 Leu Gly Val Ile Gly Val Glu Thr Arg Thr Val Glu Asn Leu Ile Pro 1890 1895 1900 Ala Asp Pro Ala Asn Pro Asn Ser Ala Glu Thr Leu Ile Gln Glu Pro 1905 1910 1915 1920 Gly Gln Val Trp His Pro Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile 1925 1930 1935 Asn Asp Phe Asn Asn Gly Glu Gln Leu Pro Met Met Ile Leu Ala Asn 1940 1945 1950 Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Phe Asn Glu Val Leu 1955 1960 1965 Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu Val Asp Tyr Lys Gln Pro 1970 1975 1980 Ile Ile Ile Tyr Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp 1985 1990 1995 2000 Val Val Val Asp Pro Thr Ile Asn Ala Asp Gln Met Glu Met Tyr Ala 2005 2010 2015 Asp Val Asn Ala Arg Ala Gly Val Leu Glu Pro Gln Gly Met Val Gly 2020 2025 2030 Ile Lys Phe Arg Arg Glu Lys Leu Leu Asp Thr Met Asn Arg Leu Asp 2035 2040 2045 Asp Lys Tyr Arg Glu Leu Arg Ser Gln Leu Ser Asn Lys Ser Leu Ala 2050 2055 2060 Pro Glu Val His Gln Gln Ile Ser Lys Gln Leu Ala Asp Arg Glu Arg 2065 2070 2075 2080 Glu Leu Leu Pro Ile Tyr Gly Gln Ile Ser Leu Gln Phe Ala Asp Leu 2085 2090 2095 His Asp Arg Ser Ser Arg Met Val Ala Lys Gly Val Ile Ser Lys Glu 2100 2105 2110 Leu Glu Trp Thr Glu Ala Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg 2115 2120 2125 Arg Leu Asn Glu Glu Tyr Leu Ile Lys Arg Leu Ser His Gln Val Gly 2130 2135 2140 Glu Ala Ser Arg Leu Glu Lys Ile Ala Arg Ile Arg Ser Trp Tyr Pro 2145 2150 2155 2160 Ala Ser Val Asp His Glu Asp Asp Arg Gln Val Ala Thr Trp Ile Glu 2165 2170 2175 Glu Asn Tyr Lys Thr Leu Asp Asp Lys Leu Lys Gly Leu Lys Leu Glu 2180 2185 2190 Ser Phe Ala Gln Asp Leu Ala Lys Lys Ile Arg Ser Asp His Asp Asn 2195 2200 2205 Ala Ile Asp Gly Leu Ser Glu Val Ile Lys Met Leu Ser Thr Asp Asp 2210 2215 2220 Lys Glu Lys Leu Leu Lys Thr Leu Lys 2225 2230 <210> 102 <211> 2248 <212> PRT <213> ACC1ke <400> 102 Met Ser Glu Glu Asn Leu Phe Glu Asp Ser Ser Ala Lys Arg Gln Tyr 1 5 10 15 Glu Ile Thr Asp Tyr Ser Lys Lys His Lys Ala Leu Ala Pro His Phe 20 25 30 Val Gly Leu Asn Thr Leu Asp Lys Val Glu Glu Ser Pro Leu Lys Asp 35 40 45 Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile 50 55 60 Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys 65 70 75 80 Trp Ser Tyr Glu Thr Phe Gly Asp Glu Arg Thr Val Gln Phe Val Ala 85 90 95 Met Ala Thr Pro Glu Asp Leu Gln Ala Asn Ser Glu Tyr Ile Arg Met 100 105 110 Ala Asp Gln Tyr Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr 115 120 125 Ala Asn Val Asp Leu Ile Val Asp Val Ala Glu Arg Thr Asp Val Asp 130 135 140 Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro His Leu Pro 145 150 155 160 Glu Lys Leu Ala Ala Ser Lys Arg Lys Ile Ile Phe Ile Gly Pro Pro 165 170 175 Gly Ser Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val 180 185 190 Ala Gln His Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Ile 195 200 205 Asp Lys Val His Val Asp Glu Thr Ser Gly Leu Val Ser Val Asp Asp 210 215 220 Asp Val Tyr Gln Gln Gly Cys Cys Gln Ser Pro Glu Asp Gly Leu Ala 225 230 235 240 Lys Ala Lys Gln Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly 245 250 255 Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile 260 265 270 Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe 275 280 285 Ile Met Lys Leu Ala Gly Lys Ala Arg His Leu Glu Val Gln Leu Leu 290 295 300 Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser 305 310 315 320 Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile 325 330 335 Ala Asn Asn Glu Thr Phe Asp Lys Met Ala Asn Ala Ala Val Arg Leu 340 345 350 Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr 355 360 365 Ser Asn Glu Glu Lys Lys Tyr Tyr Phe Leu Glu Leu Asn Pro Arg Leu 370 375 380 Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro 385 390 395 400 Ser Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser 405 410 415 Asp Ile Arg Val Leu Tyr Gly Met Asn Pro His Ser Ala Thr Glu Ile 420 425 430 Asp Phe Glu Phe Lys Thr Glu Glu Ala Lys Lys Thr Gln Arg Lys Pro 435 440 445 Val Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro 450 455 460 Asn Glu Gly Phe Lys Pro Ser Gly Gly Ser Leu His Glu Leu Asn Phe 465 470 475 480 Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly 485 490 495 Gly Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe 500 505 510 Gly Glu Asn Arg Gln Met Ser Arg Lys His Met Val Val Ala Leu Lys 515 520 525 Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile 530 535 540 Lys Leu Leu Glu Thr Glu Asp Phe Glu Glu Asn Thr Ile Thr Thr Gly 545 550 555 560 Trp Leu Asp Asp Leu Ile Ser His Lys Met Thr Ala Glu Lys Pro Asp 565 570 575 Ala Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Ile Ala 580 585 590 Ser Glu Ser Ala Arg Met Glu Tyr Ile Ala Gln Leu Lys Arg Gly Gln 595 600 605 Val Pro Ala Lys Asn Leu Leu Glu Thr Met Phe Pro Val Glu Phe Ile 610 615 620 His Glu Asn Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Ala Asp Asp 625 630 635 640 Ser Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Glu Val Arg Ala Val 645 650 655 Lys Leu Ser Asp Gly Gly Leu Leu Ile Ser Val Gly Gly Gln Ser His 660 665 670 Thr Ile Tyr Trp Lys Glu Glu Ala Gln Ala Thr Arg Leu Ser Ile Asp 675 680 685 Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg 690 695 700 Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Asp 705 710 715 720 Gln Ile Lys Val Gly Gln Ala Tyr Ala Glu Ile Glu Val Met Lys Met 725 730 735 Gln Met Pro Leu Val Ser Gln Glu Asn Gly Val Ile Gln Leu Leu Lys 740 745 750 Gln Pro Gly Ser Thr Ile Ala Ala Gly Asp Ile Ile Ala Met Leu Ala 755 760 765 Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met 770 775 780 Leu Pro Glu Leu Gly Ser Pro Met Val Glu Gly Thr Lys Pro Ala Tyr 785 790 795 800 Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr 805 810 815 Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val 820 825 830 Leu Arg Asp Pro Arg Leu Pro Tyr Ser Glu Trp Lys Leu Cys Val Ser 835 840 845 Ala Leu His Ser Arg Leu Pro Met Ser Leu Asp Glu Lys Leu Asp Gln 850 855 860 Leu Val Asp Arg Ser Ser Asn Arg Asn Ala Ile Phe Pro Ala Lys Gln 865 870 875 880 Leu Asn Lys Met Ile Glu Asp Ala Val Arg Glu Pro Gly Ser Asp Glu 885 890 895 Leu Leu Pro Thr Ile Val Glu Pro Leu Leu Asp Ile Thr Asn Arg Tyr 900 905 910 Ala Asn Gly Leu Glu Ala His Glu His Ser Val Phe Val Lys Phe Leu 915 920 925 Glu Glu Tyr Tyr Asn Val Glu Lys Leu Phe Ser Ser His Gly Asn Arg 930 935 940 Glu Glu Asn Val Ile Leu Lys Leu Arg Asp Glu Asn Leu Asp Ser Leu 945 950 955 960 Asp Lys Val Val Glu Ile Val Leu Ser His Ala Lys Val Ser Ala Lys 965 970 975 Asn Thr Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Ile Cys Lys 980 985 990 Met Ser Ser Ser Val Ala His Ile Phe Ser Lys Ser Leu Gly His Ile 995 1000 1005 Val Glu Leu Glu Ser Lys Ser Ala Ala Lys Val Ala Leu Gln Ala Arg 1010 1015 1020 Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg Thr Glu 1025 1030 1035 1040 Gln Ile Glu His Ile Leu Arg Ser Ser Val Thr Asn Thr Ser Tyr Gly 1045 1050 1055 Asp Leu Ala Pro Lys Lys Ser Glu Pro Asn Leu Glu Ile Leu Lys Asp 1060 1065 1070 Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val Leu Leu Pro Phe Leu 1075 1080 1085 Cys His Glu Asp Pro Val Ile Ala Thr Ala Ala Ala Gln Val Tyr Val 1090 1095 1100 Arg Arg Ala Tyr Arg Ala Tyr Thr Val Gly Glu Val Lys Thr Pro Asn 1105 1110 1115 1120 Ile Lys Asp Ser Ser Ile Ser Pro Leu Ile Glu Trp Lys Phe Gln Leu 1125 1130 1135 Pro Ser Ala Ala Phe Ser Ser Ile Pro Gln Val Lys Ser Lys Ile Gly 1140 1145 1150 Met Asn Arg Ala Met Ser Val Ser Asp Leu Ser Tyr Val Val Asp Asn 1155 1160 1165 Glu His Ala Pro Leu Arg Thr Gly Ile Leu Val Ala Ser Glu His Leu 1170 1175 1180 Asp Asp Val Asp Ala Cys Leu Ala Gln Gly Leu Glu Ala Ile Pro Glu 1185 1190 1195 1200 His Ala Asn Thr Ala Asn Gly Pro Ala Pro Asp Arg Ser Gly Asn Ser 1205 1210 1215 Ser Ser Leu Ser Asn Val Ala Asn Val Cys Val Ser Ser Thr Glu Gly 1220 1225 1230 Phe Glu Ser Glu Lys Asp Ile Leu Ala Arg Val His Glu Ile Leu Asp 1235 1240 1245 Leu Asn Lys Ser Asp Leu Ile Lys Ser Ser Ile Arg Arg Ile Thr Phe 1250 1255 1260 Met Phe Gly Phe Ala Asp Gly Thr Tyr Pro Lys Tyr Tyr Thr Phe Asn 1265 1270 1275 1280 Gly Pro Asn Tyr Gln Glu Asn Gln Thr Ile Arg His Ile Glu Pro Ala 1285 1290 1295 Cys Ala Phe Gln Leu Glu Leu Gly Arg Met Ser Asn Phe Asn Ile Lys 1300 1305 1310 Pro Ile Phe Thr Glu Asn Arg Asn Ile His Val Tyr Glu Ala Ile Ser 1315 1320 1325 Lys Thr Ser Ala Leu Asp Lys Arg Phe Phe Thr Arg Gly Ile Ile Arg 1330 1335 1340 Thr Gly Arg Ile Arg Asp Glu Ile Ser Ile Gln Glu Tyr Leu Thr Ser 1345 1350 1355 1360 Glu Ala Asn Lys Leu Met Ser Asp Ile Leu Asp Asn Leu Glu Ile Val 1365 1370 1375 Asp Thr Ser Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ser Ala 1380 1385 1390 Val Phe Asp Val Ser Pro Glu Asp Val Glu Ala Ala Phe Gly Gly Phe 1395 1400 1405 Leu Glu Arg Phe Gly Lys Arg Phe Leu Arg Leu Arg Val Ser Ala Ala 1410 1415 1420 Glu Ile Arg Ile Ile Ile Gln Asp Pro Lys Thr Gly Ala Ala Val Pro 1425 1430 1435 1440 Leu Arg Ala Leu Ile Asn Asn Val Ser Gly Tyr Val Val Lys Ser Glu 1445 1450 1455 Leu Tyr Thr Glu Ile Lys Asn Asp Lys Gly Glu Trp Val Phe Lys Ser 1460 1465 1470 Leu Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr 1475 1480 1485 Pro Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met 1490 1495 1500 Gly Thr Thr Tyr Leu Tyr Asp Phe Pro Glu Leu Phe His Gln Ala Ala 1505 1510 1515 1520 Ile Thr Thr Trp Glu Lys Tyr Leu Ser Asn Ser Ser Ala Lys Glu Leu 1525 1530 1535 Ser Ser Ile Lys Asp Arg Asp Asp Leu Phe Ile Ser His Glu Leu Ile 1540 1545 1550 Glu Asp Glu Asn Gly Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala 1555 1560 1565 Asn Thr Ile Gly Met Val Ala Phe Lys Val Thr Met Lys Thr Pro Glu 1570 1575 1580 Tyr Pro Arg Gly Arg Gln Phe Val Ile Val Ala Asn Asp Ile Thr Phe 1585 1590 1595 1600 Lys Ile Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val 1605 1610 1615 Thr Glu Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala 1620 1625 1630 Asn Ser Gly Ala Arg Ile Gly Ile Ala Glu Glu Leu Val Pro Leu Phe 1635 1640 1645 Gln Val Ala Trp Lys Asp Ser Lys Val Pro Ala Lys Gly Phe Glu Tyr 1650 1655 1660 Leu Tyr Leu Ser Lys Glu Gly Met Glu Thr Leu Lys Ser Tyr Ser Lys 1665 1670 1675 1680 Gln Asn Ser Val Ile Thr Glu Arg Val Val Glu Glu Gly Glu Glu Arg 1685 1690 1695 Tyr Val Ile Lys Ser Ile Ile Gly Ala Asp Asp Gly Leu Gly Val Glu 1700 1705 1710 Cys Leu Arg Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr 1715 1720 1725 Gln Asp Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly Ile 1730 1735 1740 Gly Ala Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Ile Glu Gly 1745 1750 1755 1760 Gln Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys Val Leu Gly 1765 1770 1775 Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile Met 1780 1785 1790 Tyr Asn Asn Gly Val Ser His Leu Thr Ala Ser Asp Asp Leu Gln Ala 1795 1800 1805 Val Glu Gln Ile Ile Asn Trp Leu Ser Tyr Val Pro Ala Lys Arg Asn 1810 1815 1820 Met Pro Val Pro Ile Leu Glu Thr Glu Asp Lys Trp Asp Arg Ala Ile 1825 1830 1835 1840 Asp Phe Arg Pro Thr Thr Asn Glu Gln Tyr Asp Val Arg Trp Leu Ile 1845 1850 1855 Gln Gly Arg Glu Leu Asp Asn Gly Asp Phe Glu Tyr Gly Met Phe Asp 1860 1865 1870 Lys Gly Ser Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val 1875 1880 1885 Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Ile Gly Val Ile Gly Val 1890 1895 1900 Glu Thr Arg Thr Ile Glu Asn Val Ile Pro Ala Asp Pro Ala Asn Pro 1905 1910 1915 1920 Asp Ser Thr Glu Thr Val Val Gln Glu Ala Gly Gln Val Trp Tyr Pro 1925 1930 1935 Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile Gln Asp Phe Asn His Gly 1940 1945 1950 Glu Gln Leu Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly 1955 1960 1965 Gly Gln Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe Ile 1970 1975 1980 Val Asp Ala Leu Val Asp Tyr Lys Gln Pro Ile Phe Ile Tyr Ile Pro 1985 1990 1995 2000 Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp Val Val Val Asp Pro Thr 2005 2010 2015 Ile Asn Asn Asp His Met Glu Met Tyr Ala Asp Lys Asp Ser Arg Ala 2020 2025 2030 Gly Val Leu Glu Pro Ser Gly Met Val Gly Ile Lys Tyr Arg Arg Glu 2035 2040 2045 Lys Leu Leu Ser Thr Ile Thr Arg Leu Asp Ser Thr Tyr Arg Glu Leu 2050 2055 2060 Lys Glu Lys Leu Ser Asp Lys Leu Leu Ser Thr Glu Glu His Gln Lys 2065 2070 2075 2080 Ile Ser Ile Gln Leu Ala Glu Arg Glu Lys Met Leu Ile Pro Ile Tyr 2085 2090 2095 Asn Gln Ile Ser Val Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg 2100 2105 2110 Met Leu Lys Lys Gly Val Ile Lys Ala Glu Leu Thr Trp Glu Glu Ser 2115 2120 2125 Arg Arg Tyr Phe Phe Trp Arg Leu Arg Arg Arg Leu Asp Glu Glu Tyr 2130 2135 2140 Leu Leu Arg Arg Leu Asp Ser Gln Leu Thr Gln Ala Ser Arg Leu Glu 2145 2150 2155 2160 Lys Ile Ala Arg Val Arg Ser Trp Tyr Pro Thr Ser Val Asp Val Glu 2165 2170 2175 Asn Asp Lys Glu Val Val Thr Trp Ile Glu Glu Asn Tyr Glu Thr Leu 2180 2185 2190 Glu Glu Lys Phe Lys Ala Leu Lys Leu Glu Ser Phe Ala Leu Asp Leu 2195 2200 2205 Ala Lys Asn Ile Arg Asn Asp His Glu Asn Ser Met Lys Gly Leu Glu 2210 2215 2220 Glu Val Val Arg Met Leu Ser Ala Thr Asp Lys Glu Arg Leu Asn Lys 2225 2230 2235 2240 Ile Ile Asn Pro Asp Asp Lys Lys 2245 <210> 103 <211> 2216 <212> PRT <213> ACC1mc <400> 103 Met Val Glu His Lys Ser Leu Pro Gly His Phe Leu Gly Gly Asn Ser 1 5 10 15 Leu Glu Ser Ala Pro Gln Gly Pro Val Lys Asp Phe Val Gln Ala His 20 25 30 Glu Gly His Thr Val Ile Ser Lys Val Leu Ile Ala Asn Asn Gly Met 35 40 45 Ala Ala Met Lys Glu Ile Arg Ser Val Arg Lys Trp Ala Tyr Glu Thr 50 55 60 Phe Gly Asn Glu Arg Ala Ile Glu Phe Thr Val Met Ala Thr Pro Glu 65 70 75 80 Asp Leu Lys Ala Asn Ala Glu Tyr Ile Arg Met Ala Asp Asn Phe Val 85 90 95 Glu Val Pro Gly Gly Ser Asn Asn Asn Asn Tyr Ala Asn Val Glu Leu 100 105 110 Ile Val Asp Val Ala Glu Arg Thr Ala Val His Ala Val Trp Ala Gly 115 120 125 Trp Gly His Ala Ser Glu Asn Pro Arg Leu Pro Glu Met Leu Ala Lys 130 135 140 Ser Lys His Lys Cys Leu Phe Ile Gly Pro Pro Ala Ser Ala Met Arg 145 150 155 160 Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val Ala Gln Ser Ala Gln 165 170 175 Val Pro Thr Met Gly Trp Ser Gly Asp Gly Ile Thr Glu Thr Glu Phe 180 185 190 Asp Ala Ala Gly His Val Ile Val Pro Asp Asn Ala Tyr Asn Glu Ala 195 200 205 Cys Val Lys Thr Ala Glu Gln Gly Leu Lys Ala Ala Glu Lys Ile Gly 210 215 220 Phe Pro Val Met Ile Lys Ala Ser Glu Gly Gly Gly Gly Lys Gly Ile 225 230 235 240 Arg Met Val Lys Asp Gly Ser Asn Phe Ala Gln Leu Phe Ala Gln Val 245 250 255 Gln Gly Glu Ile Pro Gly Ser Pro Ile Phe Ile Met Lys Leu Ala Gly 260 265 270 Asn Ala Arg His Leu Glu Val Gln Leu Leu Ala Asp Gln Tyr Gly Asn 275 280 285 Ala Ile Ser Leu Phe Gly Arg Asp Cys Ser Val Gln Arg Arg His Gln 290 295 300 Lys Ile Ile Glu Glu Ala Pro Val Thr Ile Ala Lys Pro Asp Val Phe 305 310 315 320 Glu Gln Met Glu Lys Ala Ala Val Arg Leu Gly Lys Leu Val Gly Tyr 325 330 335 Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr Ser His His Asp Asp Gln 340 345 350 Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Thr 355 360 365 Thr Glu Met Val Ser Gly Val Asn Leu Pro Ala Ala Gln Leu Gln Ile 370 375 380 Ala Met Gly Ile Pro Leu His Arg Ile Arg Asp Ile Arg Val Leu Tyr 385 390 395 400 Gly Val Gln Pro Asn Ser Ala Ser Glu Ile Asp Phe Gly Phe Glu His 405 410 415 Pro Thr Ser Leu Thr Ser His Arg Arg Pro Thr Pro Lys Gly His Val 420 425 430 Ile Ala Cys Arg Ile Thr Ala Glu Asn Pro Asp Ala Gly Phe Lys Pro 435 440 445 Ser Ser Gly Ile Met Gln Glu Leu Asn Phe Arg Ser Ser Thr Asn Val 450 455 460 Trp Gly Tyr Phe Ser Val Val Ser Ala Gly Gly Leu His Glu Tyr Ala 465 470 475 480 Asp Ser Gln Phe Gly His Ile Phe Ala Tyr Gly Glu Asn Arg Gln Gln 485 490 495 Ala Arg Lys Asn Met Val Ile Ala Leu Lys Glu Leu Ser Ile Arg Ala 500 505 510 Asp Phe Arg Ser Thr Val Glu Tyr Ile Ile Arg Leu Leu Glu Thr Pro 515 520 525 Asp Phe Glu Glu Asn Thr Ile Asn Thr Gly Trp Leu Asp Met Leu Ile 530 535 540 Ser Lys Lys Leu Thr Ala Glu Arg Pro Asp Thr Met Leu Ala Val Phe 545 550 555 560 Cys Gly Ala Val Thr Lys Ala His Met Ala Ser Leu Asp Cys Phe Gln 565 570 575 Gln Tyr Lys Gln Ser Leu Glu Lys Gly Gln Val Pro Ser Lys Gly Ser 580 585 590 Leu Lys Thr Val Phe Thr Val Asp Phe Ile Tyr Glu Glu Val Arg Tyr 595 600 605 Asn Phe Thr Val Thr Gln Ser Ala Pro Gly Ile Tyr Thr Leu Tyr Leu 610 615 620 Asn Gly Thr Lys Thr Gln Val Gly Ile Arg Asp Leu Ser Asp Gly Gly 625 630 635 640 Leu Leu Ile Ser Ile Asp Gly Lys Ser His Thr Thr Tyr Ser Arg Asp 645 650 655 Glu Val Gln Ala Thr Arg Met Met Val Asp Gly Lys Thr Cys Leu Leu 660 665 670 Glu Lys Glu Ser Asp Pro Thr Gln Leu Arg Ser Pro Ser Pro Gly Lys 675 680 685 Leu Val Asn Leu Leu Val Glu Asn Gly Asp His Leu Asn Ala Gly Asp 690 695 700 Ala Tyr Ala Glu Ile Glu Val Met Lys Met Tyr Met Pro Leu Ile Ala 705 710 715 720 Thr Glu Asp Gly His Val Gln Phe Ile Lys Gln Ala Gly Ala Thr Leu 725 730 735 Glu Ala Gly Asp Ile Ile Gly Ile Leu Ser Leu Asp Asp Pro Ser Arg 740 745 750 Val Lys His Ala Leu Pro Phe Asn Gly Thr Val Pro Ala Phe Gly Ala 755 760 765 Pro His Ile Thr Gly Asp Lys Pro Val Gln Arg Phe Asn Ala Thr Lys 770 775 780 Leu Thr Leu Gln His Ile Leu Gln Gly Tyr Asp Asn Gln Ala Leu Val 785 790 795 800 Gln Thr Val Val Lys Asp Phe Ala Asp Ile Leu Asn Asn Pro Asp Leu 805 810 815 Pro Tyr Ser Glu Leu Asn Ser Val Leu Ser Ala Leu Ser Gly Arg Ile 820 825 830 Pro Gln Arg Leu Glu Ala Ser Ile His Lys Leu Ala Asp Glu Ser Lys 835 840 845 Ala Ala Asn Gln Glu Phe Pro Ala Ala Gln Phe Glu Lys Leu Val Glu 850 855 860 Asp Phe Ala Arg Glu His Ile Thr Leu Gln Ser Glu Ala Thr Ala Tyr 865 870 875 880 Lys Asn Ser Val Ala Pro Leu Ser Ser Ile Phe Ala Arg Tyr Arg Asn 885 890 895 Gly Leu Thr Glu His Ala Tyr Ser Asn Tyr Val Glu Leu Met Glu Ala 900 905 910 Tyr Tyr Asp Val Glu Ile Leu Phe Asn Gln Gln Arg Glu Glu Glu Val 915 920 925 Ile Leu Ser Leu Arg Asp Gln His Lys Asp Asp Leu Asp Lys Val Leu 930 935 940 Ala Val Thr Leu Ser His Ala Lys Val Asn Ile Lys Asn Asn Val Ile 945 950 955 960 Leu Met Leu Leu Asp Leu Ile Asn Pro Val Ser Thr Gly Ser Ala Leu 965 970 975 Asp Lys Tyr Phe Thr Pro Ile Leu Lys Arg Leu Ser Glu Ile Glu Ser 980 985 990 Arg Ala Thr Gln Lys Val Thr Leu Lys Ala Arg Glu Leu Leu Ile Leu 995 1000 1005 Cys Gln Leu Pro Ser Tyr Glu Glu Arg Gln Ala Gln Met Tyr Gln Ile 1010 1015 1020 Leu Lys Asn Ser Val Thr Glu Ser Val Tyr Gly Gly Gly Ser Glu Tyr 1025 1030 1035 1040 Arg Thr Pro Ser Tyr Asp Ala Phe Lys Asp Leu Ile Asp Thr Lys Phe 1045 1050 1055 Asn Val Phe Asp Val Leu Pro His Phe Phe Tyr His Ala Asp Pro Tyr 1060 1065 1070 Ile Ala Leu Ala Ala Ile Glu Val Tyr Cys Arg Arg Ser Tyr His Ala 1075 1080 1085 Tyr Lys Ile Leu Asp Val Ala Tyr Asn Leu Glu His Lys Pro Tyr Val 1090 1095 1100 Val Ala Trp Lys Phe Leu Leu Gln Thr Ala Ala Asn Gly Ile Asp Ser 1105 1110 1115 1120 Asn Lys Arg Ile Ala Ser Tyr Ser Asp Leu Thr Phe Leu Leu Asn Lys 1125 1130 1135 Thr Glu Glu Glu Pro Ile Arg Thr Gly Ala Met Thr Ala Cys Asn Ser 1140 1145 1150 Leu Ala Asp Leu Gln Ala Glu Leu Pro Arg Ile Leu Thr Ala Phe Glu 1155 1160 1165 Glu Glu Pro Leu Pro Pro Met Leu Gln Arg Asn Ala Ala Pro Lys Glu 1170 1175 1180 Glu Arg Met Glu Asn Ile Leu Asn Ile Ala Val Arg Ala Asp Glu Asp 1185 1190 1195 1200 Met Asp Asp Thr Ala Phe Arg Thr Lys Ile Cys Glu Met Ile Thr Ala 1205 1210 1215 Asn Ala Asp Ala Phe Arg Gln Ala His Leu Arg Arg Leu Ser Val Val 1220 1225 1230 Val Cys Arg Asp Asn Gln Trp Pro Asp Tyr Tyr Thr Phe Arg Glu Arg 1235 1240 1245 Glu Asn Tyr Gln Glu Asp Glu Thr Ile Arg His Ile Glu Pro Ala Met 1250 1255 1260 Ala Tyr Gln Leu Glu Leu Ala Arg Leu Ser Asn Phe Asp Ile Lys Pro 1265 1270 1275 1280 Cys Phe Ile Glu Asn Arg Gln Met His Val Tyr Tyr Ala Val Ala Lys 1285 1290 1295 Glu Asn Pro Ser Asp Cys Arg Phe Phe Ile Arg Ala Leu Val Arg Pro 1300 1305 1310 Gly Arg Val Lys Ser Ser Met Arg Thr Ala Asp Tyr Leu Ile Ser Glu 1315 1320 1325 Ser Asp Arg Leu Leu Thr Asp Ile Leu Asp Thr Leu Glu Ile Val Ser 1330 1335 1340 His Glu Tyr Lys Asn Ser Asp Cys Asn His Leu Phe Ile Asn Phe Ile 1345 1350 1355 1360 Pro Thr Phe Ala Ile Glu Ala Asp Asp Val Glu His Ala Leu Lys Asp 1365 1370 1375 Phe Val Asp Arg His Gly Lys Arg Leu Trp Lys Leu Arg Val Thr Gly 1380 1385 1390 Ala Glu Ile Arg Phe Asn Val Gln Ser Lys Lys Pro Asp Ala Pro Ile 1395 1400 1405 Ile Pro Met Arg Phe Thr Val Asp Asn Val Ser Gly Phe Ile Leu Lys 1410 1415 1420 Val Glu Val Tyr Gln Glu Val Lys Thr Glu Lys Ser Gly Trp Ile Leu 1425 1430 1435 1440 Lys Ser Val Asn Lys Ile Pro Gly Ala Met His Met Gln Pro Leu Ser 1445 1450 1455 Thr Pro Tyr Pro Thr Lys Glu Trp Leu Gln Pro Arg Arg Tyr Lys Ala 1460 1465 1470 His Leu Met Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg 1475 1480 1485 Gln Ser Val Gln Asn Gln Trp Thr Gln Ala Ile Lys Arg Asn Pro Leu 1490 1495 1500 Leu Lys Gln Pro Ser His Leu Val Glu Ala Lys Glu Leu Val Leu Asp 1505 1510 1515 1520 Glu Asp Asp Val Leu Gln Glu Ile Asp Arg Ala Pro Gly Thr Asn Thr 1525 1530 1535 Val Gly Met Val Ala Trp Ile Met Thr Ile Arg Thr Pro Glu Tyr Pro 1540 1545 1550 Ser Gly Arg Arg Ile Ile Ala Ile Ala Asn Asp Ile Thr Phe Lys Ile 1555 1560 1565 Gly Ser Phe Gly Val Ala Glu Asp Gln Val Phe Tyr Lys Ala Ser Glu 1570 1575 1580 Leu Ala Arg Ala Leu Gly Ile Pro Arg Ile Tyr Leu Ser Ala Asn Ser 1585 1590 1595 1600 Gly Ala Arg Ile Gly Leu Ala Glu Glu Leu Ile Ser Gln Phe Arg Ala 1605 1610 1615 Ala Trp Lys Asp Ala Ser Asn Pro Thr Ala Gly Phe Asn Tyr Leu Tyr 1620 1625 1630 Leu Thr Pro Ala Glu Tyr Asp Thr Leu Val Gln Gln Gly Asp Ala Lys 1635 1640 1645 Ser Val Leu Val Glu Glu Ile Gln Asp Glu Gly Glu Thr Arg Leu Arg 1650 1655 1660 Ile Thr Asp Val Ile Gly His Thr Asp Gly Leu Gly Val Glu Asn Leu 1665 1670 1675 1680 Lys Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr Asp Asp 1685 1690 1695 Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly Ile Gly Ala 1700 1705 1710 Tyr Leu Val Arg Leu Gly Gln Arg Thr Val Gln Asn Glu Gly Gln Pro 1715 1720 1725 Ile Ile Leu Thr Gly Ala Pro Ala Leu Asn Lys Val Leu Gly Arg Glu 1730 1735 1740 Val Tyr Thr Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile Met Tyr Lys 1745 1750 1755 1760 Asn Gly Val Ser His Leu Thr Ala Glu Asn Asp Leu Glu Gly Ile Ala 1765 1770 1775 Lys Ile Val Gln Trp Leu Ser Phe Val Pro Asp Val Arg Asn Ala Pro 1780 1785 1790 Val Ser Met Arg Leu Gly Val Asp Pro Ile Asp Arg Asp Ile Glu Tyr 1795 1800 1805 Thr Pro Pro Lys Gly Pro Ser Asp Pro Arg Phe Phe Leu Ala Gly Lys 1810 1815 1820 Ser Glu Asn Gly Lys Trp Leu Ser Gly Phe Phe Asp Gln Asp Ser Phe 1825 1830 1835 1840 Ile Glu Thr Leu Ser Gly Trp Ala Arg Thr Val Val Val Gly Arg Ala 1845 1850 1855 Arg Leu Gly Gly Ile Pro Met Gly Val Val Ser Val Glu Thr Arg Thr 1860 1865 1870 Val Glu Asn Ile Val Pro Ala Asp Pro Ala Asn Ser Asp Ser Thr Glu 1875 1880 1885 Gln Val Phe Met Glu Ala Gly Gly Val Trp Phe Pro Asn Ser Ala Tyr 1890 1895 1900 Lys Thr Ala Gln Ala Ile Asn Asp Phe Asn Lys Gly Glu Gln Leu Pro 1905 1910 1915 1920 Leu Met Ile Phe Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp 1925 1930 1935 Met Tyr Asn Glu Val Leu Lys Tyr Gly Ala Gln Ile Val Asp Ala Leu 1940 1945 1950 Ser Asn Tyr Lys Gln Pro Val Phe Val Tyr Ile Ile Pro Asn Gly Glu 1955 1960 1965 Leu Arg Gly Gly Ala Trp Val Val Val Asp Pro Thr Ile Asn Lys Asp 1970 1975 1980 Met Met Glu Met Tyr Ala Asp Asn Asn Ala Arg Gly Gly Val Leu Glu 1985 1990 1995 2000 Pro Glu Gly Ile Val Glu Ile Lys Tyr Arg Lys Pro Ala Leu Leu Ala 2005 2010 2015 Thr Met Glu Arg Leu Asp Ala Thr Tyr Ala Ser Leu Lys Lys Gln Leu 2020 2025 2030 Ala Glu Glu Gly Lys Ser Asp Glu Glu Lys Ala Ala Leu Lys Val Gln 2035 2040 2045 Ile Glu Ala Arg Glu Gln Glu Leu Leu Pro Val Tyr Gln Gln Ile Ser 2050 2055 2060 Ile Gln Phe Ala Asp Leu His Asp Arg Ala Gly Arg Met Lys Ala Lys 2065 2070 2075 2080 Gly Val Ile Arg Lys Ala Leu Asp Trp Arg Arg Ala Arg His Tyr Phe 2085 2090 2095 Tyr Trp Arg Val Arg Arg Arg Leu Cys Glu Glu Tyr Thr Phe Arg Lys 2100 2105 2110 Ile Val Ala Ala Thr Ser Ala Ala Pro Met Ser Arg Glu Gln Met Leu 2115 2120 2125 Asp Leu Val Lys Gln Trp Phe Thr Ser Asp Asn Glu Thr Ala Ser Phe 2130 2135 2140 Glu Asp Ala Asp Glu Leu Val Ser Glu Trp Phe Glu Lys Arg Ala Ser 2145 2150 2155 2160 Val Ile Asp Gln Arg Ile Ser Lys Leu Lys Ser Asp Ala Thr Lys Glu 2165 2170 2175 Gln Val Val Ser Leu Gly Asn Ala Asp Gln Glu Ala Val Ile Glu Gly 2180 2185 2190 Phe Thr Gln Leu Ile Glu Asn Leu Ser Glu Asp Gln Arg Ala Glu Ile 2195 2200 2205 Leu Arg Lys Leu Asn Ser Arg Phe 2210 2215 <210> 104 <211> 2233 <212> PRT <213> ACC1sc <400> 104 Met Ser Glu Glu Ser Leu Phe Glu Ser Ser Pro Gln Lys Met Glu Tyr 1 5 10 15 Glu Ile Thr Asn Tyr Ser Glu Arg His Thr Glu Leu Pro Gly His Phe 20 25 30 Ile Gly Leu Asn Thr Val Asp Lys Leu Glu Glu Ser Pro Leu Arg Asp 35 40 45 Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile 50 55 60 Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys 65 70 75 80 Trp Ala Tyr Glu Thr Phe Gly Asp Asp Arg Thr Val Gln Phe Val Ala 85 90 95 Met Ala Thr Pro Glu Asp Leu Glu Ala Asn Ala Glu Tyr Ile Arg Met 100 105 110 Ala Asp Gln Tyr Ile Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr 115 120 125 Ala Asn Val Asp Leu Ile Val Asp Ile Ala Glu Arg Ala Asp Val Asp 130 135 140 Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro Leu Leu Pro 145 150 155 160 Glu Lys Leu Ser Gln Ser Lys Arg Lys Val Ile Phe Ile Gly Pro Pro 165 170 175 Gly Asn Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val 180 185 190 Ala Gln Ser Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val 195 200 205 Asp Thr Val His Val Asp Glu Lys Thr Gly Leu Val Ser Val Asp Asp 210 215 220 Asp Ile Tyr Gln Lys Gly Cys Cys Thr Ser Pro Glu Asp Gly Leu Gln 225 230 235 240 Lys Ala Lys Arg Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly 245 250 255 Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile 260 265 270 Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe 275 280 285 Ile Met Lys Leu Ala Gly Arg Ala Arg His Leu Glu Val Gln Leu Leu 290 295 300 Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser 305 310 315 320 Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile 325 330 335 Ala Lys Ala Glu Thr Phe His Glu Met Glu Lys Ala Ala Val Arg Leu 340 345 350 Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr 355 360 365 Ser His Asp Asp Gly Lys Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu 370 375 380 Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro 385 390 395 400 Ala Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser 405 410 415 Asp Ile Arg Thr Leu Tyr Gly Met Asn Pro His Ser Ala Ser Glu Ile 420 425 430 Asp Phe Glu Phe Lys Thr Gln Asp Ala Thr Lys Lys Gln Arg Arg Pro 435 440 445 Ile Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro 450 455 460 Asn Asp Gly Phe Lys Pro Ser Gly Gly Thr Leu His Glu Leu Asn Phe 465 470 475 480 Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly 485 490 495 Asn Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe 500 505 510 Gly Glu Asn Arg Gln Ala Ser Arg Lys His Met Val Val Ala Leu Lys 515 520 525 Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile 530 535 540 Lys Leu Leu Glu Thr Glu Asp Phe Glu Asp Asn Thr Ile Thr Thr Gly 545 550 555 560 Trp Leu Asp Asp Leu Ile Thr His Lys Met Thr Ala Glu Lys Pro Asp 565 570 575 Pro Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Leu Ala 580 585 590 Ser Glu Glu Ala Arg His Lys Tyr Ile Glu Ser Leu Gln Lys Gly Gln 595 600 605 Val Leu Ser Lys Asp Leu Leu Gln Thr Met Phe Pro Val Asp Phe Ile 610 615 620 His Glu Gly Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Gly Asn Asp 625 630 635 640 Arg Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Asp Ile Ile Leu Arg 645 650 655 Gln Leu Ser Asp Gly Gly Leu Leu Ile Ala Ile Gly Gly Lys Ser His 660 665 670 Thr Ile Tyr Trp Lys Glu Glu Val Ala Ala Thr Arg Leu Ser Val Asp 675 680 685 Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg 690 695 700 Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Glu 705 710 715 720 His Ile Ile Lys Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met 725 730 735 Gln Met Pro Leu Val Ser Gln Glu Asn Gly Ile Val Gln Leu Leu Lys 740 745 750 Gln Pro Gly Ser Thr Ile Val Ala Gly Asp Ile Met Ala Ile Met Thr 755 760 765 Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met 770 775 780 Leu Pro Asp Phe Gly Ser Pro Val Ile Glu Gly Thr Lys Pro Ala Tyr 785 790 795 800 Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr 805 810 815 Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val 820 825 830 Leu Arg Asn Pro Lys Leu Pro Tyr Ser Glu Trp Lys Leu His Ile Ser 835 840 845 Ala Leu His Ser Arg Leu Pro Ala Lys Leu Asp Glu Gln Met Glu Glu 850 855 860 Leu Val Ala Arg Ser Leu Arg Arg Gly Ala Val Phe Pro Ala Arg Gln 865 870 875 880 Leu Ser Lys Leu Ile Asp Met Ala Val Lys Asn Pro Glu Tyr Asn Pro 885 890 895 Asp Lys Leu Leu Gly Ala Val Val Glu Pro Leu Ala Asp Ile Ala His 900 905 910 Lys Tyr Ser Asn Gly Leu Glu Ala His Glu His Ser Ile Phe Val His 915 920 925 Phe Leu Glu Glu Tyr Tyr Glu Val Glu Lys Leu Phe Asn Gly Pro Asn 930 935 940 Val Arg Glu Glu Asn Ile Ile Leu Lys Leu Arg Asp Glu Asn Pro Lys 945 950 955 960 Asp Leu Asp Lys Val Ala Leu Thr Val Leu Ser His Ser Lys Val Ser 965 970 975 Ala Lys Asn Asn Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Leu 980 985 990 Cys Lys Leu Ser Ser Lys Val Ser Ala Ile Phe Ser Thr Pro Leu Gln 995 1000 1005 His Ile Val Glu Leu Glu Ser Lys Ala Thr Ala Lys Val Ala Leu Gln 1010 1015 1020 Ala Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg 1025 1030 1035 1040 Thr Glu Gln Ile Glu His Ile Leu Lys Ser Ser Val Val Lys Val Ala 1045 1050 1055 Tyr Gly Ser Ser Asn Pro Lys Arg Ser Glu Pro Asp Leu Asn Ile Leu 1060 1065 1070 Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val Leu Leu Gln 1075 1080 1085 Phe Leu Thr His Gln Asp Pro Val Val Thr Ala Ala Ala Ala Gln Val 1090 1095 1100 Tyr Ile Arg Arg Ala Tyr Arg Ala Tyr Thr Ile Gly Asp Ile Arg Val 1105 1110 1115 1120 His Glu Gly Val Thr Val Pro Ile Val Glu Trp Lys Phe Gln Leu Pro 1125 1130 1135 Ser Ala Ala Phe Ser Thr Phe Pro Thr Val Lys Ser Lys Met Gly Met 1140 1145 1150 Asn Arg Ala Val Ser Val Ser Asp Leu Ser Tyr Val Ala Asn Ser Gln 1155 1160 1165 Ser Ser Pro Leu Arg Glu Gly Ile Leu Met Ala Val Asp His Leu Asp 1170 1175 1180 Asp Val Asp Glu Ile Leu Ser Gln Ser Leu Glu Val Ile Pro Arg His 1185 1190 1195 1200 Gln Ser Ser Ser Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ala 1205 1210 1215 Ser Leu Ser Asn Val Ala Asn Val Cys Val Ala Ser Thr Glu Gly Phe 1220 1225 1230 Glu Ser Glu Glu Glu Ile Leu Val Arg Leu Arg Glu Ile Leu Asp Leu 1235 1240 1245 Asn Lys Gln Glu Leu Ile Asn Ala Ser Ile Arg Arg Ile Thr Phe Met 1250 1255 1260 Phe Gly Phe Lys Asp Gly Ser Tyr Pro Lys Tyr Tyr Thr Phe Asn Gly 1265 1270 1275 1280 Pro Asn Tyr Asn Glu Asn Glu Thr Ile Arg His Ile Glu Pro Ala Leu 1285 1290 1295 Ala Phe Gln Leu Glu Leu Gly Arg Leu Ser Asn Phe Asn Ile Lys Pro 1300 1305 1310 Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr Glu Ala Val Ser Lys 1315 1320 1325 Thr Ser Pro Leu Asp Lys Arg Phe Phe Thr Arg Gly Ile Ile Arg Thr 1330 1335 1340 Gly His Ile Arg Asp Asp Ile Ser Ile Gln Glu Tyr Leu Thr Ser Glu 1345 1350 1355 1360 Ala Asn Arg Leu Met Ser Asp Ile Leu Asp Asn Leu Glu Val Thr Asp 1365 1370 1375 Thr Ser Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ile Ala Val 1380 1385 1390 Phe Asp Ile Ser Pro Glu Asp Val Glu Ala Ala Phe Gly Gly Phe Leu 1395 1400 1405 Glu Arg Phe Gly Lys Arg Leu Leu Arg Leu Arg Val Ser Ser Ala Glu 1410 1415 1420 Ile Arg Ile Ile Ile Lys Asp Pro Gln Thr Gly Ala Pro Val Pro Leu 1425 1430 1435 1440 Arg Ala Leu Ile Asn Asn Val Ser Gly Tyr Val Ile Lys Thr Glu Met 1445 1450 1455 Tyr Thr Glu Val Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu 1460 1465 1470 Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr Pro 1475 1480 1485 Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met Gly 1490 1495 1500 Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg Gln Ala Ser Ser 1505 1510 1515 1520 Ser Gln Trp Lys Asn Phe Ser Ala Asp Val Lys Leu Thr Asp Asp Phe 1525 1530 1535 Phe Ile Ser Asn Glu Leu Ile Glu Asp Glu Asn Gly Glu Leu Thr Glu 1540 1545 1550 Val Glu Arg Glu Pro Gly Ala Asn Ala Ile Gly Met Val Ala Phe Lys 1555 1560 1565 Ile Thr Val Lys Thr Pro Glu Tyr Pro Arg Gly Arg Gln Phe Val Val 1570 1575 1580 Val Ala Asn Asp Ile Thr Phe Lys Ile Gly Ser Phe Gly Pro Gln Glu 1585 1590 1595 1600 Asp Glu Phe Phe Asn Lys Val Thr Glu Tyr Ala Arg Lys Arg Gly Ile 1605 1610 1615 Pro Arg Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg Ile Gly Met Ala 1620 1625 1630 Glu Glu Ile Val Pro Leu Phe Gln Val Ala Trp Asn Asp Ala Ala Asn 1635 1640 1645 Pro Asp Lys Gly Phe Gln Tyr Leu Tyr Leu Thr Ser Glu Gly Met Glu 1650 1655 1660 Thr Leu Lys Lys Phe Asp Lys Glu Asn Ser Val Leu Thr Glu Arg Thr 1665 1670 1675 1680 Val Ile Asn Gly Glu Glu Arg Phe Val Ile Lys Thr Ile Ile Gly Ser 1685 1690 1695 Glu Asp Gly Leu Gly Val Glu Cys Leu Arg Gly Ser Gly Leu Ile Ala 1700 1705 1710 Gly Ala Thr Ser Arg Ala Tyr His Asp Ile Phe Thr Ile Thr Leu Val 1715 1720 1725 Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val Arg Leu Gly Gln 1730 1735 1740 Arg Ala Ile Gln Val Glu Gly Gln Pro Ile Ile Leu Thr Gly Ala Pro 1745 1750 1755 1760 Ala Ile Asn Lys Met Leu Gly Arg Glu Val Tyr Thr Ser Asn Leu Gln 1765 1770 1775 Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val Ser His Leu Thr 1780 1785 1790 Ala Val Asp Asp Leu Ala Gly Val Glu Lys Ile Val Glu Trp Met Ser 1795 1800 1805 Tyr Val Pro Ala Lys Arg Asn Met Pro Val Pro Ile Leu Glu Thr Lys 1810 1815 1820 Asp Thr Trp Asp Arg Pro Val Asp Phe Thr Pro Thr Asn Asp Glu Thr 1825 1830 1835 1840 Tyr Asp Val Arg Trp Met Ile Glu Gly Arg Glu Thr Glu Ser Gly Phe 1845 1850 1855 Glu Tyr Gly Leu Phe Asp Lys Gly Ser Phe Phe Glu Thr Leu Ser Gly 1860 1865 1870 Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg Leu Gly Gly Ile Pro 1875 1880 1885 Leu Gly Val Ile Gly Val Glu Thr Arg Thr Val Glu Asn Leu Ile Pro 1890 1895 1900 Ala Asp Pro Ala Asn Pro Asn Ser Ala Glu Thr Leu Ile Gln Glu Pro 1905 1910 1915 1920 Gly Gln Val Trp His Pro Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile 1925 1930 1935 Asn Asp Phe Asn Asn Gly Glu Gln Leu Pro Met Met Ile Leu Ala Asn 1940 1945 1950 Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Phe Asn Glu Val Leu 1955 1960 1965 Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu Val Asp Tyr Lys Gln Pro 1970 1975 1980 Ile Ile Ile Tyr Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp 1985 1990 1995 2000 Val Val Val Asp Pro Thr Ile Asn Ala Asp Gln Met Glu Met Tyr Ala 2005 2010 2015 Asp Val Asn Ala Arg Ala Gly Val Leu Glu Pro Gln Gly Met Val Gly 2020 2025 2030 Ile Lys Phe Arg Arg Glu Lys Leu Leu Asp Thr Met Asn Arg Leu Asp 2035 2040 2045 Asp Lys Tyr Arg Glu Leu Arg Ser Gln Leu Ser Asn Lys Ser Leu Ala 2050 2055 2060 Pro Glu Val His Gln Gln Ile Ser Lys Gln Leu Ala Asp Arg Glu Arg 2065 2070 2075 2080 Glu Leu Leu Pro Ile Tyr Gly Gln Ile Ser Leu Gln Phe Ala Asp Leu 2085 2090 2095 His Asp Arg Ser Ser Arg Met Val Ala Lys Gly Val Ile Ser Lys Glu 2100 2105 2110 Leu Glu Trp Thr Glu Ala Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg 2115 2120 2125 Arg Leu Asn Glu Glu Tyr Leu Ile Lys Arg Leu Ser His Gln Val Gly 2130 2135 2140 Glu Ala Ser Arg Leu Glu Lys Ile Ala Arg Ile Arg Ser Trp Tyr Pro 2145 2150 2155 2160 Ala Ser Val Asp His Glu Asp Asp Arg Gln Val Ala Thr Trp Ile Glu 2165 2170 2175 Glu Asn Tyr Lys Thr Leu Asp Asp Lys Leu Lys Gly Leu Lys Leu Glu 2180 2185 2190 Ser Phe Ala Gln Asp Leu Ala Lys Lys Ile Arg Ser Asp His Asp Asn 2195 2200 2205 Ala Ile Asp Gly Leu Ser Glu Val Ile Lys Met Leu Ser Thr Asp Asp 2210 2215 2220 Lys Glu Lys Leu Leu Lys Thr Leu Lys 2225 2230 <210> 105 <211> 2266 <212> PRT <213> ACCyl <400> 105 Met Arg Leu Gln Leu Arg Thr Leu Thr Arg Arg Phe Phe Ser Met Ala 1 5 10 15 Ser Gly Ser Ser Thr Pro Asp Val Ala Pro Leu Val Asp Pro Asn Ile 20 25 30 His Lys Gly Leu Ala Ser His Phe Phe Gly Leu Asn Ser Val His Thr 35 40 45 Ala Lys Pro Ser Lys Val Lys Glu Phe Val Ala Ser His Gly Gly His 50 55 60 Thr Val Ile Asn Lys Val Leu Ile Ala Asn Asn Gly Ile Ala Ala Val 65 70 75 80 Lys Glu Ile Arg Ser Val Arg Lys Trp Ala Tyr Glu Thr Phe Gly Asp 85 90 95 Glu Arg Ala Ile Ser Phe Thr Val Met Ala Thr Pro Glu Asp Leu Ala 100 105 110 Ala Asn Ala Asp Tyr Ile Arg Met Ala Asp Gln Tyr Val Glu Val Pro 115 120 125 Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Glu Leu Ile Val Asp 130 135 140 Val Ala Glu Arg Phe Gly Val Asp Ala Val Trp Ala Gly Trp Gly His 145 150 155 160 Ala Ser Glu Asn Pro Leu Leu Pro Glu Ser Leu Ala Ala Ser Pro Arg 165 170 175 Lys Ile Val Phe Ile Gly Pro Pro Gly Ala Ala Met Arg Ser Leu Gly 180 185 190 Asp Lys Ile Ser Ser Thr Ile Val Ala Gln His Ala Lys Val Pro Cys 195 200 205 Ile Pro Trp Ser Gly Thr Gly Val Asp Glu Val Val Val Asp Lys Ser 210 215 220 Thr Asn Leu Val Ser Val Ser Glu Glu Val Tyr Thr Lys Gly Cys Thr 225 230 235 240 Thr Gly Pro Lys Gln Gly Leu Glu Lys Ala Lys Gln Ile Gly Phe Pro 245 250 255 Val Met Ile Lys Ala Ser Glu Gly Gly Gly Gly Lys Gly Ile Arg Lys 260 265 270 Val Glu Arg Glu Glu Asp Phe Glu Ala Ala Tyr His Gln Val Glu Gly 275 280 285 Glu Ile Pro Gly Ser Pro Ile Phe Ile Met Gln Leu Ala Gly Asn Ala 290 295 300 Arg His Leu Glu Val Gln Leu Leu Ala Asp Gln Tyr Gly Asn Asn Ile 305 310 315 320 Ser Leu Phe Gly Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile 325 330 335 Ile Glu Glu Ala Pro Val Thr Val Ala Gly Gln Gln Thr Phe Thr Ala 340 345 350 Met Glu Lys Ala Ala Val Arg Leu Gly Lys Leu Val Gly Tyr Val Ser 355 360 365 Ala Gly Thr Val Glu Tyr Leu Tyr Ser His Glu Asp Asp Lys Phe Tyr 370 375 380 Phe Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Thr Thr Glu 385 390 395 400 Met Val Thr Gly Val Asn Leu Pro Ala Ala Gln Leu Gln Ile Ala Met 405 410 415 Gly Ile Pro Leu Asp Arg Ile Lys Asp Ile Arg Leu Phe Tyr Gly Val 420 425 430 Asn Pro His Thr Thr Thr Pro Ile Asp Phe Asp Phe Ser Gly Glu Asp 435 440 445 Ala Asp Lys Thr Gln Arg Arg Pro Val Pro Arg Gly His Thr Thr Ala 450 455 460 Cys Arg Ile Thr Ser Glu Asp Pro Gly Glu Gly Phe Lys Pro Ser Gly 465 470 475 480 Gly Thr Met His Glu Leu Asn Phe Arg Ser Ser Ser Asn Val Trp Gly 485 490 495 Tyr Phe Ser Val Gly Asn Gln Gly Gly Ile His Ser Phe Ser Asp Ser 500 505 510 Gln Phe Gly His Ile Phe Ala Phe Gly Glu Asn Arg Ser Ala Ser Arg 515 520 525 Lys His Met Val Val Ala Leu Lys Glu Leu Ser Ile Arg Gly Asp Phe 530 535 540 Arg Thr Thr Val Glu Tyr Leu Ile Lys Leu Leu Glu Thr Pro Asp Phe 545 550 555 560 Glu Asp Asn Thr Ile Thr Thr Gly Trp Leu Asp Glu Leu Ile Ser Asn 565 570 575 Lys Leu Thr Ala Glu Arg Pro Asp Ser Phe Leu Ala Val Val Cys Gly 580 585 590 Ala Ala Thr Lys Ala His Arg Ala Ser Glu Asp Ser Ile Ala Thr Tyr 595 600 605 Met Ala Ser Leu Glu Lys Gly Gln Val Pro Ala Arg Asp Ile Leu Lys 610 615 620 Thr Leu Phe Pro Val Asp Phe Ile Tyr Glu Gly Gln Arg Tyr Lys Phe 625 630 635 640 Thr Ala Thr Arg Ser Ser Glu Asp Ser Tyr Thr Leu Phe Ile Asn Gly 645 650 655 Ser Arg Cys Asp Ile Gly Val Arg Pro Leu Ser Asp Gly Gly Ile Leu 660 665 670 Cys Leu Val Gly Gly Arg Ser His Asn Val Tyr Trp Lys Glu Glu Val 675 680 685 Gly Ala Thr Arg Leu Ser Val Asp Ser Lys Thr Cys Leu Leu Glu Val 690 695 700 Glu Asn Asp Pro Thr Gln Leu Arg Ser Pro Ser Pro Gly Lys Leu Val 705 710 715 720 Lys Phe Leu Val Glu Asn Gly Asp His Val Arg Ala Asn Gln Pro Tyr 725 730 735 Ala Glu Ile Glu Val Met Lys Met Tyr Met Thr Leu Thr Ala Gln Glu 740 745 750 Asp Gly Ile Val Gln Leu Met Lys Gln Pro Gly Ser Thr Ile Glu Ala 755 760 765 Gly Asp Ile Leu Gly Ile Leu Ala Leu Asp Asp Pro Ser Lys Val Lys 770 775 780 His Ala Lys Pro Phe Glu Gly Gln Leu Pro Glu Leu Gly Pro Pro Thr 785 790 795 800 Leu Ser Gly Asn Lys Pro His Gln Arg Tyr Glu His Cys Gln Asn Val 805 810 815 Leu His Asn Ile Leu Leu Gly Phe Asp Asn Gln Val Val Met Lys Ser 820 825 830 Thr Leu Gln Glu Met Val Gly Leu Leu Arg Asn Pro Glu Leu Pro Tyr 835 840 845 Leu Gln Trp Ala His Gln Val Ser Ser Leu His Thr Arg Met Ser Ala 850 855 860 Lys Leu Asp Ala Thr Leu Ala Gly Leu Ile Asp Lys Ala Lys Gln Arg 865 870 875 880 Gly Gly Glu Phe Pro Ala Lys Gln Leu Leu Arg Ala Leu Glu Lys Glu 885 890 895 Ala Ser Ser Gly Glu Val Asp Ala Leu Phe Gln Gln Thr Leu Ala Pro 900 905 910 Leu Phe Asp Leu Ala Arg Glu Tyr Gln Asp Gly Leu Ala Ile His Glu 915 920 925 Leu Gln Val Ala Ala Gly Leu Leu Gln Ala Tyr Tyr Asp Ser Glu Ala 930 935 940 Arg Phe Cys Gly Pro Asn Val Arg Asp Glu Asp Val Ile Leu Lys Leu 945 950 955 960 Arg Glu Glu Asn Arg Asp Ser Leu Arg Lys Val Val Met Ala Gln Leu 965 970 975 Ser His Ser Arg Val Gly Ala Lys Asn Asn Leu Val Leu Ala Leu Leu 980 985 990 Asp Glu Tyr Lys Val Ala Asp Gln Ala Gly Thr Asp Ser Pro Ala Ser 995 1000 1005 Asn Val His Val Ala Lys Tyr Leu Arg Pro Val Leu Arg Lys Ile Val 1010 1015 1020 Glu Leu Glu Ser Arg Ala Ser Ala Lys Val Ser Leu Lys Ala Arg Glu 1025 1030 1035 1040 Ile Leu Ile Gln Cys Ala Leu Pro Ser Leu Lys Glu Arg Thr Asp Gln 1045 1050 1055 Leu Glu His Ile Leu Arg Ser Ser Val Val Glu Ser Arg Tyr Gly Glu 1060 1065 1070 Val Gly Leu Glu His Arg Thr Pro Arg Ala Asp Ile Leu Lys Glu Val 1075 1080 1085 Val Asp Ser Lys Tyr Ile Val Phe Asp Val Leu Ala Gln Phe Phe Ala 1090 1095 1100 His Asp Asp Pro Trp Ile Val Leu Ala Ala Leu Glu Leu Tyr Ile Arg 1105 1110 1115 1120 Arg Ala Cys Lys Ala Tyr Ser Ile Leu Asp Ile Asn Tyr His Gln Asp 1125 1130 1135 Ser Asp Leu Pro Pro Val Ile Ser Trp Arg Phe Arg Leu Pro Thr Met 1140 1145 1150 Ser Ser Ala Leu Tyr Asn Ser Val Val Ser Ser Gly Ser Lys Thr Pro 1155 1160 1165 Thr Ser Pro Ser Val Ser Arg Ala Asp Ser Val Ser Asp Phe Ser Tyr 1170 1175 1180 Thr Val Glu Arg Asp Ser Ala Pro Ala Arg Thr Gly Ala Ile Val Ala 1185 1190 1195 1200 Val Pro His Leu Asp Asp Leu Glu Asp Ala Leu Thr Arg Val Leu Glu 1205 1210 1215 Asn Leu Pro Lys Arg Gly Ala Gly Leu Ala Ile Ser Val Gly Ala Ser 1220 1225 1230 Asn Lys Ser Ala Ala Ala Ser Ala Arg Asp Ala Ala Ala Ala Ala Ala 1235 1240 1245 Ser Ser Val Asp Thr Gly Leu Ser Asn Ile Cys Asn Val Met Ile Gly 1250 1255 1260 Arg Val Asp Glu Ser Asp Asp Asp Asp Thr Leu Ile Ala Arg Ile Ser 1265 1270 1275 1280 Gln Val Ile Glu Asp Phe Lys Glu Asp Phe Glu Ala Cys Ser Leu Arg 1285 1290 1295 Arg Ile Thr Phe Ser Phe Gly Asn Ser Arg Gly Thr Tyr Pro Lys Tyr 1300 1305 1310 Phe Thr Phe Arg Gly Pro Ala Tyr Glu Glu Asp Pro Thr Ile Arg His 1315 1320 1325 Ile Glu Pro Ala Leu Ala Phe Gln Leu Glu Leu Ala Arg Leu Ser Asn 1330 1335 1340 Phe Asp Ile Lys Pro Val His Thr Asp Asn Arg Asn Ile His Val Tyr 1345 1350 1355 1360 Glu Ala Thr Gly Lys Asn Ala Ala Ser Asp Lys Arg Phe Phe Thr Arg 1365 1370 1375 Gly Ile Val Arg Pro Gly Arg Leu Arg Glu Asn Ile Pro Thr Ser Glu 1380 1385 1390 Tyr Leu Ile Ser Glu Ala Asp Arg Leu Met Ser Asp Ile Leu Asp Ala 1395 1400 1405 Leu Glu Val Ile Gly Thr Thr Asn Ser Asp Leu Asn His Ile Phe Ile 1410 1415 1420 Asn Phe Ser Ala Val Phe Ala Leu Lys Pro Glu Glu Val Glu Ala Ala 1425 1430 1435 1440 Phe Gly Gly Phe Leu Glu Arg Phe Gly Arg Arg Leu Trp Arg Leu Arg 1445 1450 1455 Val Thr Gly Ala Glu Ile Arg Met Met Val Ser Asp Pro Glu Thr Gly 1460 1465 1470 Ser Ala Phe Pro Leu Arg Ala Met Ile Asn Asn Val Ser Gly Tyr Val 1475 1480 1485 Val Gln Ser Glu Leu Tyr Ala Glu Ala Lys Asn Asp Lys Gly Gln Trp 1490 1495 1500 Ile Phe Lys Ser Leu Gly Lys Pro Gly Ser Met His Met Arg Ser Ile 1505 1510 1515 1520 Asn Thr Pro Tyr Pro Thr Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys 1525 1530 1535 Ala His Leu Met Gly Thr Thr Tyr Cys Tyr Asp Phe Pro Glu Leu Phe 1540 1545 1550 Arg Gln Ser Ile Glu Ser Asp Trp Lys Lys Tyr Asp Gly Lys Ala Pro 1555 1560 1565 Asp Asp Leu Met Thr Cys Asn Glu Leu Ile Leu Asp Glu Asp Ser Gly 1570 1575 1580 Glu Leu Gln Glu Val Asn Arg Glu Pro Gly Ala Asn Asn Val Gly Met 1585 1590 1595 1600 Val Ala Trp Lys Phe Glu Ala Lys Thr Pro Glu Tyr Pro Arg Gly Arg 1605 1610 1615 Ser Phe Ile Val Val Ala Asn Asp Ile Thr Phe Gln Ile Gly Ser Phe 1620 1625 1630 Gly Pro Ala Glu Asp Gln Phe Phe Phe Lys Val Thr Glu Leu Ala Arg 1635 1640 1645 Lys Leu Gly Ile Pro Arg Ile Tyr Leu Ser Ala Asn Ser Gly Ala Arg 1650 1655 1660 Ile Gly Ile Ala Asp Glu Leu Val Gly Lys Tyr Lys Val Ala Trp Asn 1665 1670 1675 1680 Asp Glu Thr Asp Pro Ser Lys Gly Phe Lys Tyr Leu Tyr Phe Thr Pro 1685 1690 1695 Glu Ser Leu Ala Thr Leu Lys Pro Asp Thr Val Val Thr Thr Glu Ile 1700 1705 1710 Glu Glu Glu Gly Pro Asn Gly Val Glu Lys Arg His Val Ile Asp Tyr 1715 1720 1725 Ile Val Gly Glu Lys Asp Gly Leu Gly Val Glu Cys Leu Arg Gly Ser 1730 1735 1740 Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr Lys Asp Ile Phe Thr 1745 1750 1755 1760 Leu Thr Leu Val Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val 1765 1770 1775 Arg Leu Gly Gln Arg Ala Ile Gln Ile Glu Gly Gln Pro Ile Ile Leu 1780 1785 1790 Thr Gly Ala Pro Ala Ile Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser 1795 1800 1805 Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val 1810 1815 1820 Ser His Leu Thr Ala Arg Asp Asp Leu Asn Gly Val His Lys Ile Met 1825 1830 1835 1840 Gln Trp Leu Ser Tyr Ile Pro Ala Ser Arg Gly Leu Pro Val Pro Val 1845 1850 1855 Leu Pro His Lys Thr Asp Val Trp Asp Arg Asp Val Thr Phe Gln Pro 1860 1865 1870 Val Arg Gly Glu Gln Tyr Asp Val Arg Trp Leu Ile Ser Gly Arg Thr 1875 1880 1885 Leu Glu Asp Gly Ala Phe Glu Ser Gly Leu Phe Asp Lys Asp Ser Phe 1890 1895 1900 Gln Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val Val Gly Arg Ala 1905 1910 1915 1920 Arg Leu Gly Gly Ile Pro Phe Gly Val Ile Gly Val Glu Thr Ala Thr 1925 1930 1935 Val Asp Asn Thr Thr Pro Ala Asp Pro Ala Asn Pro Asp Ser Ile Glu 1940 1945 1950 Met Ser Thr Ser Glu Ala Gly Gln Val Trp Tyr Pro Asn Ser Ala Phe 1955 1960 1965 Lys Thr Ser Gln Ala Ile Asn Asp Phe Asn His Gly Glu Ala Leu Pro 1970 1975 1980 Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp 1985 1990 1995 2000 Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu 2005 2010 2015 Val Asp Tyr Lys Gln Pro Ile Met Val Tyr Ile Pro Pro Thr Gly Glu 2020 2025 2030 Leu Arg Gly Gly Ser Trp Val Val Val Asp Pro Thr Ile Asn Ser Asp 2035 2040 2045 Met Met Glu Met Tyr Ala Asp Val Glu Ser Arg Gly Gly Val Leu Glu 2050 2055 2060 Pro Glu Gly Met Val Gly Ile Lys Tyr Arg Arg Asp Lys Leu Leu Asp 2065 2070 2075 2080 Thr Met Ala Arg Leu Asp Pro Glu Tyr Ser Ser Leu Lys Lys Gln Leu 2085 2090 2095 Glu Glu Ser Pro Asp Ser Glu Glu Leu Lys Val Lys Leu Ser Val Arg 2100 2105 2110 Glu Lys Ser Leu Met Pro Ile Tyr Gln Gln Ile Ser Val Gln Phe Ala 2115 2120 2125 Asp Leu His Asp Arg Ala Gly Arg Met Glu Ala Lys Gly Val Ile Arg 2130 2135 2140 Glu Ala Leu Val Trp Lys Asp Ala Arg Arg Phe Phe Phe Trp Arg Ile 2145 2150 2155 2160 Arg Arg Arg Leu Val Glu Glu Tyr Leu Ile Thr Lys Ile Asn Ser Ile 2165 2170 2175 Leu Pro Ser Cys Thr Arg Leu Glu Cys Leu Ala Arg Ile Lys Ser Trp 2180 2185 2190 Lys Pro Ala Thr Leu Asp Gln Gly Ser Asp Arg Gly Val Ala Glu Trp 2195 2200 2205 Phe Asp Glu Asn Ser Asp Ala Val Ser Ala Arg Leu Ser Glu Leu Lys 2210 2215 2220 Lys Asp Ala Ser Ala Gln Ser Phe Ala Ser Gln Leu Arg Lys Asp Arg 2225 2230 2235 2240 Gln Gly Thr Leu Gln Gly Met Lys Gln Ala Leu Ala Ser Leu Ser Glu 2245 2250 2255 Ala Glu Arg Ala Glu Leu Leu Lys Gly Leu 2260 2265 <210> 106 <211> 2243 <212> PRT <213> ACC1ch <400> 106 Met Ser Glu Glu Asn Leu Phe Glu Asp Ala Ala Ala Lys Arg Gln Tyr 1 5 10 15 Glu Ile Thr Asp Tyr Ser Thr Lys His Lys Thr Leu Ala Ser His Phe 20 25 30 Val Gly Leu Asn Thr Leu Glu Arg Val Glu Glu Ser Pro Leu Lys Asp 35 40 45 Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile 50 55 60 Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys 65 70 75 80 Trp Ala Tyr Glu Thr Phe Gly Asp Glu Arg Thr Val Gln Phe Val Ala 85 90 95 Met Ala Thr Pro Glu Asp Leu Gln Ala Asn Ser Glu Tyr Ile Arg Met 100 105 110 Ala Asp Gln Tyr Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr 115 120 125 Ala Asn Val Asp Leu Ile Val Asp Val Ala Glu Arg Thr Asp Val Asp 130 135 140 Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro His Leu Pro 145 150 155 160 Glu Lys Leu Ala Ala Ser Lys Arg Lys Ile Ile Phe Ile Gly Pro Pro 165 170 175 Gly Ser Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val 180 185 190 Ala Gln His Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val 195 200 205 Asp Thr Val His Leu Asp Ser Glu Thr Gly Leu Val Ser Val Asp Asp 210 215 220 Asp Ile Tyr Gln Lys Gly Cys Cys Ser Ser Pro Glu Asp Gly Leu Ala 225 230 235 240 Lys Ala Lys Lys Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly 245 250 255 Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile 260 265 270 Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe 275 280 285 Ile Met Lys Leu Ala Gly Lys Ala Arg His Leu Glu Val Gln Leu Leu 290 295 300 Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser 305 310 315 320 Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile 325 330 335 Ala Ser Asn Glu Thr Phe Glu Lys Met Ala Ser Ala Ala Val Arg Leu 340 345 350 Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr 355 360 365 Ser Ser Glu Glu Lys Lys Tyr Tyr Phe Leu Glu Leu Asn Pro Arg Leu 370 375 380 Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro 385 390 395 400 Ser Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser 405 410 415 Asp Ile Arg Val Leu Tyr Gly Met Asn Pro His Gly Ala Thr Glu Ile 420 425 430 Asp Phe Glu Phe Lys Thr Glu Glu Ala Lys Lys Thr Gln Arg Lys Pro 435 440 445 Val Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro 450 455 460 Asn Glu Gly Phe Lys Pro Ser Gly Gly Ser Leu His Glu Leu Asn Phe 465 470 475 480 Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly 485 490 495 Gly Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe 500 505 510 Gly Glu Asn Arg Gln Met Ser Arg Lys His Met Val Val Ala Leu Lys 515 520 525 Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile 530 535 540 Lys Leu Leu Glu Thr Glu Asp Phe Glu Glu Asn Thr Ile Thr Thr Gly 545 550 555 560 Trp Leu Asp Asp Leu Ile Ser His Lys Met Thr Ala Gln Lys Pro Asp 565 570 575 Ala Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Ile Ala 580 585 590 Ser Glu Ser Ala Arg Lys Glu Tyr Ile Asn Gln Leu Lys Arg Gly Gln 595 600 605 Val Pro Pro Lys Gly Leu Leu Glu Thr Met Phe Pro Ile Glu Phe Ile 610 615 620 His Glu Asn Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Ala Asp Asp 625 630 635 640 Ser Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Glu Val Arg Ala Val 645 650 655 Lys Leu Ser Asp Gly Gly Leu Leu Ile Ser Val Gly Gly Gln Ser His 660 665 670 Thr Ile Tyr Trp Lys Glu Glu Ala Gln Ala Thr Arg Leu Ser Ile Asp 675 680 685 Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg 690 695 700 Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Asp 705 710 715 720 Gln Ile Lys Val Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met 725 730 735 Gln Met Pro Leu Val Ser Gln Glu Asn Gly Val Ile Gln Leu Leu Lys 740 745 750 Gln Pro Gly Ser Thr Ile Asp Ala Gly Asp Ile Ile Ala Met Leu Ala 755 760 765 Leu Asp Asp Pro Ser Lys Val Lys His Ala Met Pro Phe Glu Gly Met 770 775 780 Leu Pro Glu Leu Gly Ser Pro Met Val Glu Gly Thr Lys Pro Ala Tyr 785 790 795 800 Lys Phe His Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr 805 810 815 Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val 820 825 830 Leu Arg Asp Pro Arg Leu Pro Tyr Ser Glu Trp Lys Leu Asn Val Ser 835 840 845 Ala Leu His Thr Arg Leu Pro Leu Ala Leu Asp Glu Lys Leu Asp Lys 850 855 860 Leu Val Glu Arg Ser Ala Asn Arg Ser Ala Ile Phe Pro Ala Lys Gln 865 870 875 880 Leu Gln Lys Met Ile Asp Asp Ala Val Arg Asp Pro Gln Ala Asp Pro 885 890 895 Leu Leu Ser Ser Ile Val Glu Pro Leu Leu Asp Ile Thr Thr Arg Tyr 900 905 910 Ala Asn Gly Leu Glu Ala His Glu His Ser Val Phe Val Lys Phe Leu 915 920 925 Glu Glu Tyr Tyr Asn Val Glu Lys Leu Phe Ser Glu His Gly Glu Arg 930 935 940 Ala Glu Asn Val Ile Leu Lys Leu Arg Asp Glu Asn Ile Asp Asp Leu 945 950 955 960 Asp Lys Val Val Leu Ile Val Leu Ser His Ala Lys Val Ser Ala Lys 965 970 975 Asn Thr Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Ile Cys Lys 980 985 990 Met Ser Ser Ser Val Ala His Ile Phe Thr Lys Pro Leu Gln His Ile 995 1000 1005 Val Glu Leu Glu Ser Lys Pro Ala Ala Lys Val Ala Leu Gln Ala Arg 1010 1015 1020 Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg Thr Glu 1025 1030 1035 1040 Gln Ile Glu His Ile Leu Arg Ser Ser Val Thr Asn Thr Ser Tyr Gly 1045 1050 1055 Asp Ser Ser Pro Lys Val Ser Glu Pro Asn Met Glu Ile Leu Lys Asp 1060 1065 1070 Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val Leu Leu Pro Phe Leu 1075 1080 1085 Cys His Glu Asp Pro Val Ile Ala Thr Ala Ala Gly Gln Val Tyr Val 1090 1095 1100 Arg Arg Ala Tyr Arg Ala Tyr Thr Val Gly Glu Val Lys Ser His Ser 1105 1110 1115 1120 Ile Thr Asp Ser Ser Met Asn Pro Leu Ile Glu Trp Lys Phe Gln Leu 1125 1130 1135 Pro Ser Ala Ala Phe Ser Ser Ile Pro Gln Leu Lys Ser Lys Ile Gly 1140 1145 1150 Met Asn Arg Ala Met Ser Val Ser Asp Leu Ser Tyr Val Val Asp Ser 1155 1160 1165 Glu His Ala Pro Leu Arg Thr Gly Ile Leu Val Ala Ala Glu His Leu 1170 1175 1180 Asp Glu Val Asp Ala Ser Leu Ala Gln Gly Leu Glu Val Ile Pro Glu 1185 1190 1195 1200 His Ser Ala Pro Gly Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser 1205 1210 1215 Ser Ser Leu Ser Asn Ile Val Asn Val Cys Val Ala Ser Thr Glu Gly 1220 1225 1230 Phe Asp Ser Glu Lys Glu Ile Leu Ala Arg Val Asn Glu Ile Leu Glu 1235 1240 1245 Leu Asn Lys Ala Asp Leu Ile Lys Ser Ser Ile Arg Arg Ile Thr Phe 1250 1255 1260 Met Phe Gly Phe Pro Asp Gly Thr Tyr Pro Lys Tyr Tyr Thr Phe Asn 1265 1270 1275 1280 Gly Pro Asn Tyr Gln Glu Asn Gln Thr Ile Arg His Ile Glu Pro Ala 1285 1290 1295 Cys Ala Phe Gln Leu Glu Leu Gly Arg Met Ser Asn Phe Asn Ile Thr 1300 1305 1310 Pro Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr Glu Ala Val Ser 1315 1320 1325 Lys Thr Ser Ala Leu Asp Lys Arg Phe Phe Thr Arg Gly Ile Ile Arg 1330 1335 1340 Thr Gly Arg Ile Arg Asp Glu Ile Ser Ile Gln Glu Tyr Leu Thr Ser 1345 1350 1355 1360 Glu Ala Asn Lys Leu Met Ser Asp Ile Leu Asp Asn Leu Glu Ile Ile 1365 1370 1375 Asp Thr Ser Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ser Ala 1380 1385 1390 Val Phe Asp Val Ser Pro Glu Asp Val Glu Ala Ala Phe Gly Gly Phe 1395 1400 1405 Leu Glu Arg Phe Gly Lys Arg Phe Leu Arg Leu Arg Val Ser Ala Ala 1410 1415 1420 Glu Ile Arg Ile Ile Ile Gln Asp Pro Lys Thr Gly Ala Pro Val Pro 1425 1430 1435 1440 Leu Arg Ala Leu Ile Asn Asn Val Ser Gly Tyr Val Val Lys Ser Glu 1445 1450 1455 Leu Tyr Thr Glu Ile Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser 1460 1465 1470 Leu Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr 1475 1480 1485 Pro Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met 1490 1495 1500 Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe His Gln Ala Ala 1505 1510 1515 1520 Ile Thr Thr Trp Gln Lys Tyr Lys Thr Asn Ser Ser Gln Lys Asp Leu 1525 1530 1535 Thr Thr Met Lys Glu Arg Asp Asp Leu Phe Ile Ser His Glu Leu Ile 1540 1545 1550 Glu Asp Glu Ser Gly Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala 1555 1560 1565 Asn Ser Ile Gly Met Val Ala Phe Lys Val Thr Met Lys Thr Pro Glu 1570 1575 1580 Tyr Pro Arg Gly Arg Gln Phe Val Ile Val Ala Asn Asp Ile Thr Tyr 1585 1590 1595 1600 Lys Ile Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val 1605 1610 1615 Thr Glu Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala 1620 1625 1630 Asn Ser Gly Ala Arg Ile Gly Ile Ala Glu Glu Leu Val Pro Leu Tyr 1635 1640 1645 Gln Val Ala Trp Lys Asp Ala Asp Val Pro Ala Lys Gly Phe Glu Tyr 1650 1655 1660 Ile Tyr Leu Asn Lys Asp Glu Met Glu Thr Leu Lys Gly His Ser Lys 1665 1670 1675 1680 Glu Asn Ser Val Ile Thr Glu Arg Ile Val Glu Asp Gly Glu Glu Arg 1685 1690 1695 Phe Val Ile Lys Ala Ile Ile Gly Ala Asp Asp Gly Leu Gly Val Glu 1700 1705 1710 Cys Leu Arg Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr 1715 1720 1725 Gln Asp Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly Ile 1730 1735 1740 Gly Ala Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Ile Glu Gly 1745 1750 1755 1760 Gln Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys Val Leu Gly 1765 1770 1775 Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile Met 1780 1785 1790 Tyr Asn Asn Gly Val Ser His Leu Thr Ala Ser Asp Asp Leu Gln Ala 1795 1800 1805 Val Glu Gln Ile Ile Lys Trp Leu Ser Tyr Val Pro Ala Lys Arg Asn 1810 1815 1820 Met Pro Val Pro Ile Leu Glu Asn Gly Asp Lys Trp Asp Arg Gln Ile 1825 1830 1835 1840 Asp Tyr Arg Pro Thr Val Asn Glu Gln Tyr Asp Val Arg Trp Met Ile 1845 1850 1855 Glu Gly Arg Glu Leu Glu Asp Gly Arg Phe Glu Tyr Gly Met Phe Asp 1860 1865 1870 Lys Gly Ser Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val 1875 1880 1885 Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Ile Gly Val Ile Gly Val 1890 1895 1900 Glu Thr Arg Thr Ile Glu Asn Val Ile Pro Ala Asp Pro Ala Asn Pro 1905 1910 1915 1920 Asp Ser Thr Glu Ala Val Val Gln Glu Ala Gly Gln Val Trp Tyr Pro 1925 1930 1935 Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile Lys Asp Phe Asn His Gly 1940 1945 1950 Glu Gln Leu Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly 1955 1960 1965 Gly Gln Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe Ile 1970 1975 1980 Val Asp Ser Leu Val Asp Tyr Lys Gln Pro Ile Phe Ile Tyr Ile Pro 1985 1990 1995 2000 Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp Val Val Val Asp Pro Thr 2005 2010 2015 Ile Asn Ala Asp Gln Met Glu Met Tyr Ala Asp Lys Asp Ser Arg Ala 2020 2025 2030 Gly Val Leu Glu Pro Ser Gly Met Val Gly Ile Lys Tyr Arg Arg Glu 2035 2040 2045 Lys Leu Leu Ala Thr Met Thr Arg Leu Asp Thr Thr Tyr Arg Glu Leu 2050 2055 2060 Lys Glu Gln Leu Ser Asp Lys Thr Leu Thr Pro Ala Gln His Gln Glu 2065 2070 2075 2080 Val Ser Val Lys Leu Ala Glu Arg Glu Lys Phe Leu Leu Pro Ile Tyr 2085 2090 2095 Thr Gln Val Ser Val Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg 2100 2105 2110 Met Val Lys Lys Gly Val Ile Arg Ala Glu Leu Thr Trp Glu Glu Ser 2115 2120 2125 Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg Lys Leu Asp Glu Glu Tyr 2130 2135 2140 Leu Val Lys Arg Leu Asp Ile Glu Leu Pro His Ala Ser Arg Leu Glu 2145 2150 2155 2160 Lys Leu Ala Arg Leu Arg Ser Trp Tyr Pro Ala Ser Ile Asp Glu Thr 2165 2170 2175 Lys Asp Arg Asp Val Val Thr Trp Ile Glu Glu Asn Tyr Asn Val Phe 2180 2185 2190 Glu Glu Glu Phe Lys Ala Leu Lys Leu Glu Ser Phe Ala Gln Glu Leu 2195 2200 2205 Ala Lys Ser Met Arg Thr Asp His Asp Val Thr Met Arg Gly Leu Glu 2210 2215 2220 Glu Ile Ala Arg Met Leu Ser Lys Glu Asp Lys Glu Arg Leu Leu Ser 2225 2230 2235 2240 Ala Leu Lys <210> 107 <211> 1229 <212> PRT <213> HPDH-MCRbs <400> 107 Met Thr Ala Gly Lys Gly Lys Gly Ala Lys Val Asp Pro Ala Gly Gly 1 5 10 15 Lys Ala Ala Thr Gly Arg Leu Ala Gly Lys Val Ala Leu Val Thr Gly 20 25 30 Ala Ala Gly Asn Leu Gly Gly Ala Ile Ile Arg Arg Phe Ile Ala Glu 35 40 45 Gly Ala Thr Leu Val Met Thr Gly Arg Thr Lys Glu Arg Leu Ala Ala 50 55 60 Ala Lys Gln Ala Leu Leu Asp Glu Thr Gly Val Glu Glu Ala Arg Val 65 70 75 80 Phe Thr Val Ile Leu Asp Gly Ala Asp Pro Asp Ser Val Arg Ala Gly 85 90 95 Ile Asp Gln Val Arg Ala Ala Leu Gly Arg Ile Asp Val Leu Val Asn 100 105 110 Asn Ala Gly Ser Ala Gly Pro Arg Gln Thr Leu Glu Asn Val Pro Leu 115 120 125 Thr Arg Ala Glu Met Glu Ala Asn Gly Asp Ser Glu Thr Val Ala Asp 130 135 140 Ala Met Arg Asn Leu Leu Gly Val Ser Trp Asn Leu Thr Arg Ala Ala 145 150 155 160 Ala Pro Ile Met Pro Val Gly Ala Ser Val Val Asn Ile Ser Thr Ile 165 170 175 Phe Ser His Thr Arg Tyr Tyr Gly Arg Thr Ala Tyr Val Val Pro Lys 180 185 190 Ala Ala Leu Asn Ala Leu Ser Gln Glu Leu Ala Lys Glu Leu Gly Ala 195 200 205 Ser Gly Ile Arg Val Asn Thr Val Phe Pro Gly Pro Ile Glu Ser Glu 210 215 220 Arg Ile Arg Thr Val Phe Ala Ala Met Asp Ala Leu Gln Gly Glu Pro 225 230 235 240 Glu Asn Ser Thr Ala Asn His Phe Met Gly Arg Met Thr Leu Glu Arg 245 250 255 Ala Ile Gly Asp Ala Pro Lys Arg Lys Thr Leu Pro Val Pro Gly Asp 260 265 270 Ile Ala Asp Ala Cys Leu Phe Leu Ala Ser Asp Glu Ser Ala Ala Leu 275 280 285 Asn Gly Asn Glu Ile Asp Val Thr His Gly Met Gln Val Arg Lys Asp 290 295 300 Ser Arg Ser Thr Tyr Met Thr Arg Pro Ser Met Arg Ser Leu Asp Gly 305 310 315 320 Ala Gly Leu Thr Val Leu Ile Ala Ala Gly Glu Ala Trp Glu Asp Ala 325 330 335 Leu Asp Met Ala Lys Ile Gln Ala Ala Cys Gly Ala Arg Val Leu Leu 340 345 350 Gly Met Thr Arg Gln Val Asp Val Ala Gln Ala Glu Ala Arg Val Lys 355 360 365 Ala Gln Gly Ala Glu Glu Gly Leu Thr Ile Met Arg Phe Asn Arg Ala 370 375 380 Glu Pro Asp Gly Met Glu Ala Ala Ile Arg Ala Tyr Ser Asp Ala His 385 390 395 400 Gly Ala Ile His Ser Ala Ile Val Leu Pro Val Lys Pro Ala Gly His 405 410 415 Phe Gly Asp Ser Leu Val Glu Ala Ser Asp Ala Glu Val Asp Ala Leu 420 425 430 Ile Asp Ile Glu Leu Thr Gly Ala Ile Ala Val Ala Arg Thr Leu Arg 435 440 445 Arg Tyr Trp Lys Thr Cys Ser Asp Leu Met Gln Asp Pro Arg Phe Val 450 455 460 Phe Met Ser Gly Pro Ser Asp Gly Lys Gly Asn Lys Ala Gly Arg Ile 465 470 475 480 Leu Ala Ala Ala Ile Ala Gln Leu Ile Thr Ile Trp Arg Asp Glu Ser 485 490 495 Arg Val Asp Phe Ala Gln Gly Arg Gln Lys Arg Ala Ala Trp Gly Asn 500 505 510 Gln Ile Val Arg Tyr Thr Asn Glu Glu Asp Glu Asn Thr Arg Phe Ala 515 520 525 Ala Gly His Ala Thr Arg Ile Val Phe Lys Glu Gln Lys Ile Thr Glu 530 535 540 Thr Ser Leu Tyr Leu Pro Ala Asn Ile Gly Glu Glu Thr Gly Ala Arg 545 550 555 560 Arg Ala Met Leu Gly Phe Ala Glu Asn Ile Thr Gly Leu His Leu Gly 565 570 575 Lys Val Ala Met Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Val 580 585 590 Ala Arg Leu Leu Ala Leu Ala Gly Ala Lys Val Met Met Val Ala Arg 595 600 605 Arg Ala Ser Glu Leu Glu Ala Ala Arg Glu Arg Ile Val Gly Glu Leu 610 615 620 Gln Asp Val Gly Phe Ser Gly Val Glu Arg Arg Val Lys Ile Met Ala 625 630 635 640 Asp Val Asp Val Ser Asp Phe Asp Ser Leu Arg Arg Ala Ala Asp Ala 645 650 655 Thr Met Ala Glu Phe Gly Arg Ile Asp Tyr Leu Ile Asn Asn Ala Gly 660 665 670 Val Ala Gly Ala Glu Asp Met Val Ile Asp Met Ser Leu Asp Met Trp 675 680 685 Arg Trp Thr Leu Asp Ala Asn Leu Ile Ser Asn Tyr Leu Leu Met His 690 695 700 Tyr Val Val Pro Ile Met Lys Arg Gln Gly Ser Gly Tyr Ile Leu Asn 705 710 715 720 Val Ser Ser Tyr Phe Gly Gly Glu Lys Phe Leu Ala Val Ala Tyr Pro 725 730 735 Asn Arg Ala Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Val 740 745 750 Glu Ser Phe Ser Arg Phe Leu Gly Pro Glu Ile Gln Val Asn Ala Ile 755 760 765 Ala Pro Gly Pro Val Asp Gly Asp Arg Leu Ser Gly Thr Gly Gly Lys 770 775 780 Pro Gly Leu Phe Leu Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg 785 790 795 800 Leu Asn Ala Ile Tyr Ala Ala Val Ile Glu Ser Ile Arg Glu Gly Ala 805 810 815 Glu Val Thr His Val Leu Gly Arg Leu Ala Arg Asn Asp Thr Gly Tyr 820 825 830 Leu Ser His Asp Arg Ser Thr Pro Asp Pro Leu Arg Leu Leu Cys Leu 835 840 845 Glu Leu Ala Arg Glu Gly Asp Gly Ile Cys Thr Trp Asp Arg Tyr Leu 850 855 860 Leu Thr Pro Asp Ile Ala Gly Arg Leu Leu Met Arg Leu Ala Ser Ala 865 870 875 880 Gly Tyr Leu Leu Ala His Glu Glu Trp Gln Gln Arg Ser Ala Ser Ala 885 890 895 Glu Gly Arg Thr Gly Trp Leu Leu Ala Leu Pro Pro Asp Asp Met Pro 900 905 910 Tyr Leu Pro Gln Glu Arg Ile Gly Lys Glu Ala Asp Lys Val Gly Arg 915 920 925 Gly Val Leu Ser Gln Leu His Leu Gly Ala Met Pro Lys Glu Ser Glu 930 935 940 Val Ala Gln Ala Thr Val Phe Tyr Leu Ala Asp Arg Ala Val Ser Gly 945 950 955 960 Glu Thr Phe Met Pro Ser Gly Gly Leu Ser Val Glu Arg Ser Tyr Thr 965 970 975 Glu Arg Glu Met Phe Gly Ser Pro Lys Gln Glu Arg Leu Glu Gln Met 980 985 990 Lys Gly Lys Thr Val Trp Val Ile Gly Glu His Leu Ala Asp Tyr Ile 995 1000 1005 Ala Glu Thr Ala Asn His Leu Ile Gly Gly Leu Gly Val Ala Arg Leu 1010 1015 1020 Val Leu Leu Thr Arg Ser Glu Ala Gly Gly Ala Ser Val Leu Ala Leu 1025 1030 1035 1040 Leu Asp Pro Glu Ile Ala Glu Arg Val Thr Val Arg Leu Cys Gly Ala 1045 1050 1055 Asp Val Glu Ser Ala Met Asp Gln Ala Leu Thr Asp Phe Gly Arg Pro 1060 1065 1070 Thr Thr Val Val Ser Thr Pro Met Ala Gly Leu Pro Asp Arg Leu Phe 1075 1080 1085 Glu Asp Gly Ala Met Leu Glu Pro Glu Glu Phe Arg Thr Leu Cys Glu 1090 1095 1100 Glu Asn Leu Thr Gln His Phe Arg Val Ser Arg Lys Ala Ser Leu Tyr 1105 1110 1115 1120 Asp Gly Cys Gln Leu Val Leu Val Ser Pro Asp Val Pro Phe Gly Gln 1125 1130 1135 Ser Gly Pro Ala Phe Ala Leu Ala Asn Phe Val Lys Thr Thr Leu His 1140 1145 1150 Ala Phe Thr Ala Thr Leu Ala Val Glu Asn Glu Arg Leu Val His Asp 1155 1160 1165 Val Pro Thr Asn Gln Ile Asn Leu Thr Arg Arg Val Arg Ser Glu Glu 1170 1175 1180 Pro Arg Asn Ala Val Glu His Gln Glu Glu Leu Lys Arg Phe Ala Arg 1185 1190 1195 1200 Ala Val Leu Leu Val Gly Ala Pro Leu Pro Asp Ala Gln Asp Ser Arg 1205 1210 1215 Tyr Arg Ala Arg Ile Tyr Arg Gly Thr Ser Met Thr Val 1220 1225 <210> 108 <211> 1220 <212> PRT <213> HPDH-MCRca <400> 108 Met Ser Gly Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly 1 5 10 15 Gly Ala Gly Asn Ile Gly Ser Glu Leu Thr Arg Arg Phe Leu Ala Glu 20 25 30 Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Arg Ala Lys Leu Thr Ala 35 40 45 Leu Ala Glu Arg Met Gln Ala Glu Ala Gly Val Pro Ala Lys Arg Ile 50 55 60 Asp Leu Glu Val Met Asp Gly Ser Asp Pro Val Ala Val Arg Ala Gly 65 70 75 80 Ile Glu Ala Ile Val Ala Arg His Gly Gln Ile Asp Ile Leu Val Asn 85 90 95 Asn Ala Gly Ser Ala Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu 100 105 110 Thr Glu Ala Glu Leu Gly Pro Gly Ala Glu Glu Thr Leu His Ala Ser 115 120 125 Ile Ala Asn Leu Leu Gly Met Gly Trp His Leu Met Arg Ile Ala Ala 130 135 140 Pro His Met Pro Val Gly Ser Ala Val Ile Asn Val Ser Thr Ile Phe 145 150 155 160 Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Thr Pro Lys Ala 165 170 175 Ala Leu Asn Ala Leu Ser Gln Leu Ala Ala Arg Glu Leu Gly Ala Arg 180 185 190 Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Asp Arg 195 200 205 Ile Arg Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu 210 215 220 Gly Asp Thr Ala His His Phe Leu Asn Thr Met Arg Leu Cys Arg Ala 225 230 235 240 Asn Asp Gln Gly Ala Leu Glu Arg Arg Phe Pro Ser Val Gly Asp Val 245 250 255 Ala Asp Ala Ala Val Phe Leu Ala Ser Ala Glu Ser Ala Ala Leu Ser 260 265 270 Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Ala Cys Ser 275 280 285 Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Ser 290 295 300 Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met 305 310 315 320 Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly 325 330 335 Phe Arg Ser Ala Ala Ala Leu Ala Gln Phe Glu Gln Ala Val Asn Glu 340 345 350 Ser Arg Arg Leu Ala Gly Ala Asp Phe Thr Pro Pro Ile Ala Leu Pro 355 360 365 Leu Asp Pro Arg Asp Pro Ala Thr Ile Asp Ala Val Phe Asp Trp Gly 370 375 380 Ala Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala 385 390 395 400 Thr Ser His Glu Pro Ala Pro Cys Val Ile Glu Val Asp Asp Glu Arg 405 410 415 Val Leu Asn Phe Leu Ala Asp Glu Ile Thr Gly Thr Ile Val Ile Ala 420 425 430 Ser Arg Leu Ala Arg Tyr Trp Gln Ser Gln Arg Leu Thr Pro Gly Ala 435 440 445 Arg Ala Arg Gly Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Asp Gln 450 455 460 Asn Gly Asn Val Tyr Gly Arg Ile Gln Ser Ala Ala Ile Gly Gln Leu 465 470 475 480 Ile Arg Val Trp Arg His Glu Ala Glu Leu Asp Tyr Gln Arg Ala Ser 485 490 495 Ala Ala Gly Asp His Val Leu Pro Pro Val Trp Ala Asn Gln Ile Val 500 505 510 Arg Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp 515 520 525 Thr Ala Gln Leu Leu His Ser Gln Arg His Ile Asn Glu Ile Thr Leu 530 535 540 Asn Ile Pro Ala Asn Ile Ser Ala Thr Thr Gly Ala Arg Ser Ala Ser 545 550 555 560 Val Gly Trp Ala Glu Ser Leu Ile Gly Leu His Leu Gly Lys Val Ala 565 570 575 Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu 580 585 590 Leu Ala Leu Ser Gly Ala Arg Val Met Leu Ala Ala Arg Asp Arg His 595 600 605 Lys Leu Glu Gln Met Gln Ala Met Ile Gln Ser Glu Leu Ala Glu Val 610 615 620 Gly Tyr Thr Asp Val Glu Asp Arg Val His Ile Ala Pro Gly Cys Asp 625 630 635 640 Val Ser Ser Glu Ala Gln Leu Ala Asp Leu Val Glu Arg Thr Leu Ser 645 650 655 Ala Phe Gly Thr Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly 660 665 670 Val Glu Glu Met Val Ile Asp Met Pro Val Glu Gly Trp Arg His Thr 675 680 685 Leu Phe Ala Asn Leu Ile Ser Asn Tyr Ser Leu Met Arg Lys Leu Ala 690 695 700 Pro Leu Met Lys Lys Gln Gly Ser Gly Tyr Ile Leu Asn Val Ser Ser 705 710 715 720 Tyr Phe Gly Gly Glu Lys Asp Ala Ala Ile Pro Tyr Pro Asn Arg Ala 725 730 735 Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Ala Glu Val Phe 740 745 750 Ala Arg Phe Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly 755 760 765 Pro Val Glu Gly Asp Arg Leu Arg Gly Thr Gly Glu Arg Pro Gly Leu 770 775 780 Phe Ala Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu 785 790 795 800 Leu His Ala Ala Leu Ile Ala Ala Ala Arg Thr Asp Glu Arg Ser Met 805 810 815 His Glu Leu Val Glu Leu Leu Leu Pro Asn Asp Val Ala Ala Leu Glu 820 825 830 Gln Asn Pro Ala Ala Pro Thr Ala Leu Arg Glu Leu Ala Arg Arg Phe 835 840 845 Arg Ser Glu Gly Asp Pro Ala Ala Ser Ser Ser Ser Ala Leu Leu Asn 850 855 860 Arg Ser Ile Ala Ala Lys Leu Leu Ala Arg Leu His Asn Gly Gly Tyr 865 870 875 880 Val Leu Pro Ala Asp Ile Phe Ala Asn Leu Pro Asn Pro Pro Asp Pro 885 890 895 Phe Phe Thr Arg Ala Gln Ile Asp Arg Glu Ala Arg Lys Val Arg Asp 900 905 910 Gly Ile Met Gly Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe Asp 915 920 925 Val Ala Met Ala Thr Val Tyr Tyr Leu Ala Asp Arg Asn Val Ser Gly 930 935 940 Glu Thr Phe His Pro Ser Gly Gly Leu Arg Tyr Glu Arg Thr Pro Thr 945 950 955 960 Gly Gly Glu Leu Phe Gly Leu Pro Ser Pro Glu Arg Leu Ala Glu Leu 965 970 975 Val Gly Ser Thr Val Tyr Leu Ile Gly Glu His Leu Thr Glu His Leu 980 985 990 Asn Leu Leu Ala Arg Ala Tyr Leu Glu Arg Tyr Gly Ala Arg Gln Val 995 1000 1005 Val Met Ile Val Glu Thr Glu Thr Gly Ala Glu Thr Met Arg Arg Leu 1010 1015 1020 Leu His Asp His Val Glu Ala Gly Arg Leu Met Thr Ile Val Ala Gly 1025 1030 1035 1040 Asp Gln Ile Glu Ala Ala Ile Asp Gln Ala Ile Thr Arg Tyr Gly Arg 1045 1050 1055 Pro Gly Pro Val Val Cys Thr Pro Phe Arg Pro Leu Pro Thr Val Pro 1060 1065 1070 Leu Val Gly Arg Lys Asp Ser Asp Trp Ser Thr Val Leu Ser Glu Ala 1075 1080 1085 Glu Phe Ala Glu Leu Cys Glu His Gln Leu Thr His His Phe Arg Val 1090 1095 1100 Ala Arg Lys Ile Ala Leu Ser Asp Gly Ala Ser Leu Ala Leu Val Thr 1105 1110 1115 1120 Pro Glu Thr Thr Ala Thr Ser Thr Thr Glu Gln Phe Ala Leu Ala Asn 1125 1130 1135 Phe Ile Lys Thr Thr Leu His Ala Phe Thr Ala Thr Ile Gly Val Glu 1140 1145 1150 Ser Glu Arg Thr Ala Gln Arg Ile Leu Ile Asn Gln Val Asp Leu Thr 1155 1160 1165 Arg Arg Ala Arg Ala Glu Glu Pro Arg Asp Pro His Glu Arg Gln Gln 1170 1175 1180 Glu Leu Glu Arg Phe Ile Glu Ala Val Leu Leu Val Thr Ala Pro Leu 1185 1190 1195 1200 Pro Pro Glu Ala Asp Thr Arg Tyr Ala Gly Arg Ile His Arg Gly Arg 1205 1210 1215 Ala Ile Thr Val 1220 <210> 109 <211> 1219 <212> PRT <213> HPDH-MCRcag <400> 109 Met Ser Val Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly 1 5 10 15 Gly Ala Gly Asn Ile Gly Ser Glu Met Thr Arg Arg Phe Leu Ala Glu 20 25 30 Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Ser Ala Lys Leu Ala Ala 35 40 45 Leu Ala Glu Arg Leu Arg Ser Glu Ala Gly Val Pro Ala Lys Arg Ile 50 55 60 Asp Leu Glu Val Met Asp Gly Ser Asp Pro Ala Ala Val Arg Ala Gly 65 70 75 80 Val Ala Ala Ile Ile Gly Arg His Gly His Ile Asp Ile Leu Val Asn 85 90 95 Asn Ala Gly Ser Thr Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu 100 105 110 Asn Glu Thr Asp Arg Asp Leu Asp Asp Glu Glu Ala Leu Ser Thr Ser 115 120 125 Val Ala Asn Leu Leu Gly Met Ala Trp His Leu Met Arg Ile Leu Ser 130 135 140 Pro His Met Pro Pro Gly Ser Ala Ile Ile Asn Ile Ser Thr Ile Phe 145 150 155 160 Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ala 165 170 175 Ala Leu Asn Thr Leu Thr Gln Ile Ala Ala Arg Glu Leu Gly Ile Arg 180 185 190 Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Glu Arg 195 200 205 Ile Gln Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu 210 215 220 Gly Asp Thr Ala Ser Gln Phe Leu Ala Thr Met Arg Leu Tyr Arg Ala 225 230 235 240 Asn Asp Gln Gly Gln Leu Glu Arg Arg Phe Pro Thr Ile Cys Asp Val 245 250 255 Ala Asp Ala Ala Val Phe Leu Ala Ser Asp Glu Ala Ala Ala Leu Thr 260 265 270 Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Thr Ser Ser 275 280 285 Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Asn 290 295 300 Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met 305 310 315 320 Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly 325 330 335 Phe Arg Ser Glu Ala Ala Leu Ala Gln Phe Glu Gln Ala Ile Gly Glu 340 345 350 Ser Arg Arg Leu Ala Gly Glu Ser Phe Ile Pro Pro Ile Ala Leu Pro 355 360 365 Ile Asp Leu Arg Asn Pro Ser Thr Ile Asp Ala Leu Phe Asp Trp Ala 370 375 380 Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala Ser 385 390 395 400 Gly Arg Glu Pro Ala Thr Gln Val Ile Asp Ile Asp Asp Ala His Val 405 410 415 Gln Ala Phe Leu Asn Asp Glu Ile Val Gly Ser Ile Ile Ile Ala Ser 420 425 430 Arg Leu Ala Arg Tyr Trp Gln Ala Gln Arg Ile Ala Pro Gly Ala Arg 435 440 445 Ala Arg Glu Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Ser Thr Ala 450 455 460 Gly Asn Pro Tyr Gly Arg Ile Gln Ser Ala Ala Ile Glu Gln Leu Ile 465 470 475 480 Arg Val Trp Arg His Glu Ala Ala Leu Asp Tyr Glu Arg Ala Thr Ala 485 490 495 Ala Gly Glu Arg Val Leu Pro Ala Val Trp Ala Ser Gln Ile Val Arg 500 505 510 Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp Thr 515 520 525 Ala Gln Leu Leu His Ser Gln Arg Arg Ile Asn Glu Ile Thr Leu Thr 530 535 540 Ile Pro Ala Asp Ile Ser Ala Thr Thr Gly Ala Arg Ser Ala Ser Val 545 550 555 560 Gly Trp Ala Glu Ser Leu Ile Gly Leu His Leu Gly Lys Val Ala Leu 565 570 575 Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu Leu 580 585 590 Ala Leu Ser Gly Ala Arg Val Met Leu Ala Ala Arg Asp Pro His Lys 595 600 605 Leu Glu Gln Ile Gln Ala Thr Ile Arg Ala Glu Leu Ala Glu Val Gly 610 615 620 Tyr Thr Asp Val Glu Glu Arg Val Gln Ile Ala Pro Gly Cys Asp Val 625 630 635 640 Ser Ser Glu Glu Gln Leu Val Asp Leu Val Glu Arg Thr Leu Ala Ala 645 650 655 Phe Gly Thr Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly Val 660 665 670 Glu Glu Met Val Ile Asp Met Pro Val Glu Gly Trp Arg Asn Thr Leu 675 680 685 Tyr Ala Asn Leu Ile Ser Asn Tyr Ser Leu Met Arg Lys Leu Ala Pro 690 695 700 Leu Met Lys Lys Gln Gly Ser Gly Tyr Val Leu Asn Val Ser Ser Tyr 705 710 715 720 Phe Gly Gly Glu Lys Asp Ala Ala Ile Pro Tyr Pro Asn Arg Ala Asp 725 730 735 Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Ala Glu Val Phe Ala 740 745 750 Arg Phe Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly Pro 755 760 765 Val Glu Gly Asp Arg Leu Arg Gly Thr Gly Glu Arg Pro Gly Leu Phe 770 775 780 Ala Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu Leu 785 790 795 800 His Ala Ala Leu Ile Thr Ala Ala Arg Thr Asp Asn Arg Pro Met Arg 805 810 815 Glu Leu Val Glu Leu Leu Leu Pro Asn Asp Val Ala Ala Leu Ala Gln 820 825 830 His Pro Ala Ala Pro Asp Val Leu Arg Thr Leu Ala Lys Arg Phe Gln 835 840 845 Ser Glu Gly Asp Pro Ala Ala Ser Ser Ser Ser Phe Leu Leu Asn Arg 850 855 860 Ser Ile Ala Ala Lys Leu Leu Ala Arg Leu Ile Asn Gly Gly Tyr Asp 865 870 875 880 Leu Pro Ala Asp Ile Phe Ala Asn Leu Ala Val Pro Pro Asp Pro Phe 885 890 895 Phe Thr Arg Ala Gln Ile Asp Arg Glu Ala Arg Lys Val Arg Asp Gly 900 905 910 Ile Met Gly Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe Asp Val 915 920 925 Ala Met Ala Thr Val Tyr Tyr Leu Ala Asp Arg Asn Val Ser Gly Glu 930 935 940 Thr Phe His Pro Ser Gly Gly Leu Arg Tyr Glu Arg Thr Pro Thr Gly 945 950 955 960 Gly Glu Leu Phe Gly Leu Pro Ala Pro Glu Arg Leu Ala Glu Leu Val 965 970 975 Gly Ser Thr Val Tyr Leu Ile Gly Glu His Leu Thr Glu His Leu Asn 980 985 990 Leu Leu Ala Arg Ala Tyr Leu Glu Arg Tyr Gly Ala Arg Gln Val Val 995 1000 1005 Met Ile Val Glu Thr Glu Ala Gly Ala Glu Lys Met Arg His Leu Leu 1010 1015 1020 His Asp His Val Glu Ala Gly Arg Leu Pro Ile Ile Val Ala Gly Asp 1025 1030 1035 1040 Gln Ile Glu Ala Ala Ile Asp Gln Ala Ile Ala Asn Tyr Gly Arg Pro 1045 1050 1055 Gly Pro Val Val Cys Thr Pro Phe Arg Pro Leu Pro Ser Ala Pro Leu 1060 1065 1070 Val Gly Arg Lys Asp Ser Asp Trp Ser Thr Val Leu Ser Glu Ala Glu 1075 1080 1085 Phe Ala Glu Leu Cys Glu His Gln Leu Thr His His Phe Arg Val Ala 1090 1095 1100 Arg Lys Ile Ala Leu Ser Asp Gly Ala Ser Leu Ala Leu Val Thr Pro 1105 1110 1115 1120 Glu Thr Thr Ala Thr Ser Ser Thr Glu Gln Phe Ala Leu Ala Asn Phe 1125 1130 1135 Val Lys Thr Thr Leu His Ala Phe Thr Ala Thr Ile Gly Val Glu Ser 1140 1145 1150 Glu Arg Thr Ala Gln Arg Ile Leu Ile Asn Gln Val Asp Leu Thr Arg 1155 1160 1165 Arg Ala Arg Ala Glu Glu Pro Arg Asp Pro Arg Glu Arg Gln Gln Glu 1170 1175 1180 Leu Glu Arg Phe Ile Glu Ala Val Leu Leu Val Thr Ala Pro Leu Pro 1185 1190 1195 1200 Pro Glu Ala Asp Thr Arg Tyr Ala Gly Arg Ile His Arg Gly Arg Ala 1205 1210 1215 Ile Thr Val <210> 110 <211> 1231 <212> PRT <213> HPDH-MCRct <400> 110 Met Arg Arg Thr Lys Pro Glu Cys Leu Arg Arg Asn Glu Ser Thr Met 1 5 10 15 Arg Leu Asn Gly Lys Val Ala Leu Ile Thr Gly Ala Ala Gly Gly Ile 20 25 30 Gly Ser Val Val Thr Arg Met Tyr Leu Arg Glu Gly Ala Arg Val Ala 35 40 45 Ile Ser Ser Arg Ser Leu Glu Arg Ala Glu Gln Phe Arg Ala Ala Leu 50 55 60 Met Ala Glu Gly Phe Ser Gly Asp Asp Ile Leu Pro Val Ala Phe Ala 65 70 75 80 Ser Thr Asp Leu Ala Gly Met Gln Thr Ala Ile Glu Ala Ile Ala Ala 85 90 95 Arg Trp Gly Gly Leu Asp Val Leu Ile Asn Asn Ala Gly Ser Ala Gly 100 105 110 Ala Lys Gln Pro Leu Phe Arg Ile Pro Phe Thr Ser Glu Asp Leu Ala 115 120 125 Gln Leu Ala Ala Glu Gly Phe Thr Glu Thr Glu Thr Met Arg Glu Ser 130 135 140 Ala Ala Asn Leu Leu Gly Met Pro Trp His Leu Thr Arg Leu Ala Leu 145 150 155 160 Pro Tyr Leu Arg Val Gly Ala Ser Ile Ile Asn Val Ser Thr Ile Phe 165 170 175 Ser Arg Thr Asn Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ser 180 185 190 Ala Leu Asn Ala Leu Ser Leu Gly Leu Ala Lys Gln Leu Gly Thr Thr 195 200 205 Asp Arg Ala Val Arg Val Asn Thr Val Phe Pro Gly Pro Ile Asp Ser 210 215 220 Asp Arg Ile Arg Thr Val Phe Gly Ala Met Asp Arg Leu Lys Gly Val 225 230 235 240 Pro Glu Gly Thr Thr Ala Gln Glu Phe Phe Asp Ile Met Ile Leu Ala 245 250 255 Arg Ala Arg Gly Gly Glu Ala Pro Thr Lys Cys Tyr Pro Thr Gln Ala 260 265 270 Asp Val Ala His Val Ile Leu Phe Leu Gly Ser Asp Glu Ser Ala Ala 275 280 285 Ile Ser Gly His Asn Phe Glu Ile Thr His Gly Met Gln Val Lys Ala 290 295 300 Gln Ser Arg Thr Lys Leu Thr Ala Trp Pro Asp Gln Arg Leu Ile Asp 305 310 315 320 Leu Ser Arg Arg Val Val Leu Ile Val Gly Gly Glu Gln Val His Asp 325 330 335 Ala Val Thr Ile Ala Ala Ser Gln Gln Ser Tyr Gly Ala Ser Val Ile 340 345 350 Leu Thr Phe Arg Gln Pro Glu Ala Val Asn Thr Ala Lys Ala Gln Leu 355 360 365 Glu Ser Arg Gly Leu Asn His Ser Ile Ala Val Thr Trp Leu Asp Pro 370 375 380 Leu Arg Arg Glu Ser Val Thr Ser Val Phe Asp Leu Ile Arg Glu Arg 385 390 395 400 Tyr His Arg Leu Asp Thr Val Ile Val Phe Pro Ala His Ala Ala Gly 405 410 415 His Tyr Gly Pro Glu Leu Ile Pro Asp Glu Pro Glu Arg Ile Arg Gln 420 425 430 Phe Thr Asp Glu Ala Ile Leu Gly Thr Thr Ala Phe Ala Ala Gln Leu 435 440 445 Ser Arg Phe Leu Leu Ala Phe Asp Ala Glu Asn Glu Leu Gln Glu Pro 450 455 460 Val Asn Val Leu Phe Val Thr Asn Glu His Asp Gly Ala Thr Asn Ala 465 470 475 480 Phe Glu Asp Ile Tyr Arg Ala Ala Val Glu Gln Leu Leu Arg Val Trp 485 490 495 Arg His Glu Asp Glu Leu Gln Val Ala Ala Gly Thr Arg Arg Phe Ala 500 505 510 Val Arg Thr Asn Gln Ile Val Arg Tyr Ala Asn Ala Glu Pro Asp Asn 515 520 525 Leu Lys Phe Thr Ala Asp Trp Leu Ala Thr Leu Ala Asn Arg Val Arg 530 535 540 Ile Phe Asp Glu Leu Asn Leu Tyr Val Pro Glu Asn Ile Gln Arg Thr 545 550 555 560 Thr Gly Lys Thr Glu Leu Pro Arg Asp Ile Ala Arg Thr Leu Leu Gly 565 570 575 Leu His Ile Gly Lys Val Ala Val Ile Thr Gly Gly Ser Ala Gly Ile 580 585 590 Gly Gly Ala Ile Gly His Tyr Leu Ala Leu Ser Gly Ala Lys Val Val 595 600 605 Leu Ala Ala Arg Asp Ala Glu Arg Leu Ala Ala Leu Lys Arg Glu Ile 610 615 620 Val Ala Glu Leu Phe Ala Val Gly Tyr Pro Gln Pro Glu Thr Arg Val 625 630 635 640 Glu Thr Leu Ala Asn Ile Asp Val Ala Asp Glu Glu Ala Leu Gly Arg 645 650 655 Leu Val Lys Phe Ala Leu Asp Lys Phe Gly Arg Ile Asp Phe Leu Ile 660 665 670 Asn Asn Ala Gly Ile Ala Gly Ala Glu Glu Met Val Val Asp Leu Pro 675 680 685 Leu Ala Ala Trp Arg His Thr Leu Lys Ala Asn Leu Leu Ser Asn Tyr 690 695 700 Ser Leu Ile Tyr Lys Ile Ala Pro Leu Met Lys Lys Gln Gly Ala Gly 705 710 715 720 Tyr Ile Leu Asn Val Ser Ser Tyr Phe Gly Gly Glu Lys Tyr Val Ala 725 730 735 Val Ala Tyr Pro Asn Arg Ser Asp Tyr Ala Val Ser Lys Ala Gly Gln 740 745 750 Arg Ala Leu Ala Glu Ile Leu Ala Arg His Leu Gly Pro Glu Ile Gln 755 760 765 Ile Asn Ala Leu Ser Pro Gly Pro Val Glu Gly Lys Arg Leu Arg Gly 770 775 780 Glu Gly Ser Arg Pro Gly Leu Tyr Ala Arg Arg Ala Arg Leu Ile Leu 785 790 795 800 Glu Asn Lys Arg Leu Asn Asp Ile His Asp Ala Val Ile Arg Ala Trp 805 810 815 Arg Glu Gly Glu Ala Val Lys Ser Thr Leu Gly Ala Leu Ala His Asn 820 825 830 His Phe Glu Asp Val Leu Ala Ala Gly Pro Pro Glu Pro Val Arg Arg 835 840 845 Leu Ile Thr His Leu Arg Ala Ala Ala Val Asn Glu Gly His Ser Asp 850 855 860 Ser His Tyr Leu Met Thr Arg Lys Ile Ala Glu Lys Leu Leu His Arg 865 870 875 880 Leu Phe Glu Gly Gly Tyr Leu Thr Ser Val Glu Asp Arg Glu Ala Phe 885 890 895 Thr Leu Ala Phe Leu Arg Glu Leu Pro Glu Pro Pro Asp Pro Phe Phe 900 905 910 Asp Pro Lys Glu Ile Asp Arg Glu Ala Glu Arg Val Gln Ser Gly Ile 915 920 925 Leu Thr Met Leu Asn Leu Arg Arg Met Pro Thr Glu Glu Glu Ile Ala 930 935 940 Ile Ala Thr Val Phe Tyr Leu Ser Asp Pro Asn Ile Ser Gly Glu Thr 945 950 955 960 Leu His Pro Ser Gly Gly Leu Lys Phe Asp Arg Thr Val Thr Glu Gly 965 970 975 Glu Phe Tyr Gly Leu Pro Arg Asn Glu His Leu Met Glu Leu Arg Arg 980 985 990 Lys Asn Val Leu Leu Ile Gly Asp Tyr Leu Glu Glu Glu Leu Arg Trp 995 1000 1005 Leu Val Gly Ala Phe Ala Ile Gln Leu Val Val Arg Asn Cys Ile Ile 1010 1015 1020 Leu Thr Lys Thr Ala Glu Arg Ala Asp Ser Leu Arg His Tyr Phe Ser 1025 1030 1035 1040 Ala Gln Arg Leu Asn Asn Leu His Ile Phe Ser Ala Gly Ala Asn Val 1045 1050 1055 Glu Ala Ala Met Asp Asp Leu Ile Arg Arg Phe Gly His Leu Asp Val 1060 1065 1070 Val Val Ser Thr Pro Phe Asp Pro Leu Pro Leu Lys Pro Leu Ala Ala 1075 1080 1085 Thr Asp Asp Trp Arg Gly Val Leu Asn Glu Lys Asp Phe Glu Gln Leu 1090 1095 1100 Val Glu Thr His Ile Thr His His Phe Arg Val Ala Lys Lys Ala Ala 1105 1110 1115 1120 Leu Gln Asp Lys Ser Gln Ile Val Leu Val Thr Pro Lys Thr Ser Arg 1125 1130 1135 Asn Ser Ser Arg Glu Glu Phe Ala Leu Ala Leu Phe Val Lys Thr Thr 1140 1145 1150 Leu His Ala Leu Thr Ala Thr Leu Ala Val Glu Cys Glu Arg Phe Thr 1155 1160 1165 His His Pro Thr Val Asn Gln Val Asp Leu Thr Arg Arg Ala Arg Val 1170 1175 1180 Glu Glu Pro Arg Asn Glu Arg Glu Glu Arg Glu Glu Leu Glu Arg Phe 1185 1190 1195 1200 Val Asp Ala Val Leu Leu Ala Ala Ala Pro Ala Pro Glu Pro Glu Gln 1205 1210 1215 Ser Arg Tyr Leu Ser Arg Ile His Arg Gly Asn Ala Ile Thr Val 1220 1225 1230 <210> 111 <211> 1216 <212> PRT <213> HPDH-MCRgb <400> 111 Met Asn Asp Met Ser Asp Ile Pro Gln Asp Leu Leu Thr Gln Ser Ile 1 5 10 15 Ala Gly Arg Arg Leu Asp Asn Lys Cys Ile Ile Leu Thr Gly Ala Ala 20 25 30 Gly Ser Ile Gly Ser Phe Ile Thr Arg Gln Leu Leu Arg Glu Gly Ala 35 40 45 Arg Val Met Leu Thr Gly Arg Asp Gln Ser Lys Leu Asp Asp Phe Ile 50 55 60 Gly Asp Leu Val Ser Glu Gly Phe Glu Glu Thr Phe Met Val Ser Ala 65 70 75 80 Thr Gly Asp Cys Ala Asp Pro Glu Val Cys Arg Glu Ile Val Ala Lys 85 90 95 Thr Val Ala Ala Phe Gly Pro Ile Asp Val Leu Val Asn Asn Ala Gly 100 105 110 Ala Ala Gly Pro Lys Phe Thr Leu Arg Asp Ile Pro Phe Thr Glu Ala 115 120 125 Glu Gln Arg Ala Ala Asp Ser Asp Gln Thr Met Phe Asp Ser Ala Met 130 135 140 Asn Leu Leu Gly Ala Pro Trp Asn Met Ala Arg Ala Ala Ala Pro His 145 150 155 160 Met Ser Ala Gly Ala Ser Ile Ile Asn Val Ser Thr Ile Phe Ser Arg 165 170 175 Thr His Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ser Gly Leu 180 185 190 Asn Ala Leu Gly Lys Gly Leu Ala Leu Glu Leu Gly Glu Lys Gln Gly 195 200 205 Ile Arg Val Asn Thr Leu Phe Pro Gly Pro Ile Glu Ser Glu Arg Ile 210 215 220 Glu Ser Val Phe Ala Arg Met Asp Glu Leu Gln Gly Val Glu Pro Gly 225 230 235 240 Ser Thr Gly Lys Glu Phe Arg Asp Leu Met Ile Thr Thr Arg Thr Ser 245 250 255 Asp Gly Gly Leu Glu Tyr Lys Tyr Pro Thr Pro Thr Asp Val Ala Ser 260 265 270 Gly Ile Val Trp Leu Ala Ser Asp Glu Ser Ser Ala Leu Ser Gly His 275 280 285 His Ile Glu Val Thr Asn Gly Met Gln Val Pro Ala Gln Ser Arg Ser 290 295 300 Gln Leu Val Ser Trp Pro Asp Lys Arg Leu Glu Asp Leu Ser Gly Gln 305 310 315 320 Val Val Leu Ile Leu Gly Gly Asp Asp Tyr Gln Glu Ala Val Thr Phe 325 330 335 Ala Glu Arg Gln Ile Gln Ser Gly Ala Arg Val Met Leu Ala Phe Arg 340 345 350 Asn Leu Asn Thr Val Gly His Ala Arg Ser Leu Val Gln Ser Arg Asn 355 360 365 Leu Gly Asp Val Gln Leu Ser His Leu Asp Pro Leu Arg Arg Glu Ser 370 375 380 Val Asp Arg Ser Met Gln Leu Ile Ala Asp His Phe Gly Arg Leu Asp 385 390 395 400 Gly Val Ile Leu Leu Pro Gln Lys Pro Asn Gly Glu Tyr Gly Tyr Ser 405 410 415 Leu Thr Thr Ala Asn Asp Glu Asp Val Ala Asn Phe Val Arg Asp Glu 420 425 430 Val Val Ala Pro Val Ala Phe Ala Ser Ala Phe Ala Arg Asn Met Asp 435 440 445 Gln Leu Phe Ala Glu Gly Glu Pro Pro Ala Ile Val Tyr Val Thr Asn 450 455 460 Pro Ser Asp Arg His Gly Asn Leu Met Asn Glu Ile Ile Arg Ala Ser 465 470 475 480 Val Glu Ala Leu Ile Arg Gly Trp Arg His Glu Asp Glu Thr Leu Ala 485 490 495 Asn Thr Gly Glu Leu Pro Trp Ala Val Gln Pro Asn Gln Met Val Arg 500 505 510 Tyr Asp Thr Glu Asp Arg Glu Ala Leu Thr Phe Ala Ala Asp Trp Ala 515 520 525 Ala Thr Leu Thr Asn Arg Val Arg Lys Met Asp Ser Ile Asn Leu Trp 530 535 540 Leu Pro Arg Ser Ile Lys Arg Ser Thr Gly Lys Ser Ser Met Pro Ser 545 550 555 560 Ser Ile Ser Arg Val Leu Pro Gly Leu His Lys Gly Arg Thr Ala Val 565 570 575 Ile Thr Gly Gly Ser Leu Gly Ile Gly Leu Gln Leu Gly Arg Phe Leu 580 585 590 Ala Ile Ala Gly Ala Arg Val Leu Leu Ser Ala Arg Ser Glu Gly Lys 595 600 605 Leu Ala Glu Ala Arg Asp Glu Ile Val Asp Glu Leu Arg Asn Ile Gly 610 615 620 Tyr Pro Arg Pro Glu Ser Arg Val Thr Ile Leu Gly Gly Ile Asp Val 625 630 635 640 Gly Asp Pro Asp Ala Leu Asp Arg Leu His Asp His Ala Val Ala Glu 645 650 655 Leu Gly His Val Asp Phe Leu Ile Asn Asn Ala Gly Ile Ser Gly Ala 660 665 670 Glu Glu Met Val Val Asp Met Thr Arg Ala Asp Trp Asp Arg Thr Met 675 680 685 Glu Ala Asn Leu Ile Ser Asn Tyr Ser Leu Ile Arg Lys Phe Ser Pro 690 695 700 Ala Met Lys Ala Gly Gly Lys Gly Ser Ile Leu Asn Val Ser Ser Tyr 705 710 715 720 Phe Gly Gly Glu Lys Tyr Val Ala Val Ala Tyr Pro Asn Arg Ala Asp 725 730 735 Tyr Ala Val Ser Lys Ala Gly Gln Arg Val Leu Ala Glu Ile Leu Ser 740 745 750 Arg His Leu Gly Pro Glu Ile Gln Ile Asn Ala Leu Ala Pro Gly Pro 755 760 765 Val Asp Gly Ala Arg Leu Arg Gly Ser Ala Glu Ala Pro Gly Leu Phe 770 775 780 Asp Arg Arg Gly Arg Leu Val Leu Glu Asn Lys Arg Leu Asn Glu Val 785 790 795 800 His Lys Ala Ile Leu Ala Gly Met Ser Ser Gly Phe Asp Ala Asp Gly 805 810 815 Val Met Ser Leu Ala Ser Asn Ala Val Asp Ser Ile Asp Ile Asp Ser 820 825 830 Leu Pro Lys Pro Val Ala Arg Leu Val Leu Lys Ile Arg Asp Ser Gly 835 840 845 Gly Met Gly Asn Ser Ser His Tyr Leu Met His Met Gly Ile Ala Ala 850 855 860 Lys Leu Met Thr Arg Leu Val Arg Ala Gly Ile Leu Ser Asp Glu Gln 865 870 875 880 Arg Glu Gln Phe Leu Gly Thr Phe Ile Asp Ala Pro Ala Pro Phe Phe 885 890 895 Asp Phe Ser Glu Thr Ser Lys Gln Ala Glu Gln Ile Glu Thr Gly Ile 900 905 910 Leu Asn Arg Leu His Leu His Lys Met Pro Thr Asp Glu Gln Val Gly 915 920 925 Leu Ser Thr Val Phe His Leu Ala Asp Asp Ile Val Ser Gly Glu Thr 930 935 940 Phe His Pro Ser Gly Gly Leu Lys Phe Asp Arg Ser Val Thr Glu Gly 945 950 955 960 Glu Leu Leu Leu Pro Pro Asn Glu Thr Glu Val Ala Lys Leu Lys Gly 965 970 975 Lys Arg Ile Val Leu Ile Gly Asp Ser Met Arg Asn Glu Leu Thr His 980 985 990 Ile Ala Asn Gly Phe Leu Ala Gln Asn Val Glu Lys Leu Trp Val Leu 995 1000 1005 Thr Lys Thr Glu Asp Ser Ala Asn Ala Met Thr His Ser Val Asp Asn 1010 1015 1020 Pro His Gly Ile Asp Ile Glu Cys Arg Ala Ile Gly Asp Asp Leu Glu 1025 1030 1035 1040 Ser Ser Leu Asp Asp Ile Leu Arg Asn Asp Gly Gly Tyr Asp Val Val 1045 1050 1055 Val Ser Ser Pro Phe Glu Arg Leu Pro Leu Asn Ala Leu Ala Ala Asn 1060 1065 1070 Ala Asn Glu Pro Trp Asp Arg Val Leu Ser Asp His Glu Phe Arg Lys 1075 1080 1085 Leu Val His Asp Gln Leu Thr His His Phe Arg Val Ala Arg Thr Ser 1090 1095 1100 Ala Leu Val Pro Asn Cys Gln Ile Val Leu Ile Thr Pro Asp Thr Ser 1105 1110 1115 1120 Leu Ala Ser Thr Arg Glu Glu Phe Ala Leu Ala Leu Phe Val Lys Asn 1125 1130 1135 Ser Leu His Ala Phe Thr Val Thr Leu Gly Val Glu Gly Glu Arg Leu 1140 1145 1150 Pro Thr Val Pro Ala Ile Asn Gln Val Gln Leu Thr Arg Arg Ala His 1155 1160 1165 Thr Glu Glu Pro Ser Asn Asp Gln Glu Leu Glu Glu Glu Met Thr Arg 1170 1175 1180 Leu Val His Ala Val Leu Gln Cys Ser Val Pro Ala Pro Ala Pro Ser 1185 1190 1195 1200 Asp Ser Arg Tyr Leu Ser Lys Ile Phe Arg Gly Asn Ala Val Thr Val 1205 1210 1215 <210> 112 <211> 1221 <212> PRT <213> HPDH-MCRot <400> 112 Met Phe Met Thr Arg Leu Asn Asp Lys Ile Ala Leu Ile Thr Gly Gly 1 5 10 15 Ala Gly Thr Ile Gly Glu Val Ile Thr Arg Arg Tyr Leu Glu Glu Gly 20 25 30 Ala Thr Val Val Met Ala Gly Arg Asn Arg Asp Lys Leu Asp Arg Tyr 35 40 45 Arg Glu Arg Leu Ile Thr Glu Phe His Ala Leu Pro Glu Arg Val Met 50 55 60 Val Val Arg Met Asp Gly Ser Ser Asn Ala Glu Val Arg Met Gly Ile 65 70 75 80 Ala Ala Val Val Ala His Phe Gly Arg Ile Asp Ile Leu Val Asn Asn 85 90 95 Ala Gly Ser Ala Gly Ala Arg Gln Arg Leu Pro Ala Ile Pro Leu Leu 100 105 110 Arg Ser Glu Leu Gln Ala Asp Glu Thr Glu Thr Leu Ala Asp Ser Ile 115 120 125 Gly Asn Leu Ile Gly Ile Thr Trp Asn Leu Ile Arg Ala Ala Ala Pro 130 135 140 Phe Met Pro Ala Gly Ser Ser Val Ile Asn Ile Ser Thr Ile Phe Ala 145 150 155 160 Arg Thr Asp Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ala Ala 165 170 175 Leu His Ala Leu Thr Leu Ala Ala Ala Thr Glu Leu Gly Glu Arg Gly 180 185 190 Ile Arg Val Asn Gln Ile Asn Pro Gly Pro Ile Asp Ser Asp Arg Ile 195 200 205 Arg Thr Val Phe Arg Arg Met Asp Glu Leu Lys Gly Val Pro Glu Gln 210 215 220 Ser Thr Ala Asp Gly Phe Phe Gln Met Met Arg Leu Arg Arg Pro Asn 225 230 235 240 Ala Glu Gly Asp Leu Val Lys Gly Phe Pro Lys Thr Leu Asp Val Ala 245 250 255 Asn Val Ala Val Phe Leu Gly Ser Ala Glu Ser Ala Ala Leu Ser Gly 260 265 270 Glu Thr Leu Asp Val Thr His Gly Met Ala Val Pro Thr Glu Ser Arg 275 280 285 Thr Thr Leu Thr Ser Arg Pro Gly Leu Arg Ala Val Asp Gly Ser Gly 290 295 300 His Thr Thr Leu Ile Cys Val Gly Asp Gln Ile Glu Glu Ala Ala Ala 305 310 315 320 Leu Thr Gly Val Leu Arg Ala Cys Gly Ala Glu Val Val Ile Gly Phe 325 330 335 Arg Ser Arg Ala Ala Ile Ala Arg Phe Asp His Leu Ile Glu Arg Gly 340 345 350 Arg His Leu Pro Ser Gln Glu His Val Ala Pro Val Leu Leu Tyr Leu 355 360 365 Asn Pro Thr Glu Pro Glu Ser Ile Asp Gln Ala Leu Arg Trp Met Ala 370 375 380 Thr Asn Leu Asp Leu Pro Thr Ser Val Ile Ile Leu Pro Ala Gln Arg 385 390 395 400 Gln Pro Leu Pro Pro Ser Val Val Arg Ala Ser Asp Glu Glu Val Ala 405 410 415 Tyr Phe Leu Arg Asp Glu Leu Ser Gly Met Ile Val Leu Ala Ser Arg 420 425 430 Leu Ala Arg Phe Trp Gln Gln Ala Thr Leu Ala Pro Gly Asn Ala Pro 435 440 445 Ile Gln Pro Arg Val Leu Phe Met Thr Asn Pro Asp Asp Gly Gln Gly 450 455 460 Asn Leu Tyr Ala Glu Ile Leu Arg Ala Gly Val Glu Gln Leu Cys Arg 465 470 475 480 Val Trp Arg His Glu Ser Gln Leu Asp Tyr Thr Arg Leu Ala Gln Met 485 490 495 Asp Ala His Pro Pro His Ile Arg Pro Val Trp Ala Asn Gln Leu Val 500 505 510 Arg Phe Ala Asn Asn Glu Gln Glu Asn Leu Glu Tyr Cys Cys Ala Trp 515 520 525 Val Ala Lys Ile Leu Leu Ser Glu Arg Thr Ile Glu Glu Leu Asn Leu 530 535 540 Tyr Leu Pro Arg Gln Ile Gly Ser Thr Thr Gly Ser Arg Gln Pro Ser 545 550 555 560 Phe Gly Trp Ala Glu Asn Leu Ile Gly Leu His Leu Gly Lys Thr Ala 565 570 575 Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Ser Gln Ile Ala Arg Leu 580 585 590 Leu Ala Leu Ser Gly Ala Arg Val Met Leu Cys Ala Arg Asp Glu Arg 595 600 605 Lys Leu Ile Gln Met Arg Asp Met Ile Ile Ala Glu Leu Thr Glu Val 610 615 620 Gly Tyr Asn Gln Val Glu Ser Arg Val Gln Ile Cys Ala Gly Cys Asp 625 630 635 640 Val Gly Glu Glu Glu Gln Leu Glu Ile Ala Val Gln Arg Thr Leu Asp 645 650 655 Leu Phe Gly His Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly 660 665 670 Ala Glu Glu Met Val Leu Asp Leu Pro Leu Glu Ala Trp Gln Arg Thr 675 680 685 Leu Arg Thr Asn Leu Ile Ser Asn Tyr Ser Leu Ile Arg Lys Leu Ala 690 695 700 Pro Gln Met Lys Ser Arg Gly Ser Gly Tyr Ile Leu Asn Val Ser Ser 705 710 715 720 Tyr Phe Gly Gly Glu Lys Tyr Ala Ala Ile Pro Tyr Pro Asn Arg Ala 725 730 735 Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Leu Gly Glu Ala Leu 740 745 750 Ala Arg Leu Leu Gly Pro Glu Val Gln Ile Asn Ala Met Ala Pro Gly 755 760 765 Pro Val Glu Gly Glu Arg Leu Arg Gly Ser Gly Asp Arg Pro Gly Leu 770 775 780 Phe Leu Arg Arg Gly Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Asp 785 790 795 800 Leu His Ala Thr Leu Ile Ala Ala Glu Arg Glu Thr Gln Val Gly Met 805 810 815 Arg Asp Leu Leu Ala Arg Leu Leu His Asn Asp Val Cys Ala Leu Ile 820 825 830 Asp Asp Pro Ala Ala Pro Thr His Leu Arg Ala Leu Ala Glu Arg Ile 835 840 845 Trp Glu Gln Ser Asp Pro Asn Ser Tyr Ala Arg Ala Phe Phe Met Asn 850 855 860 Ala Asn Ile Ala Thr Lys Leu Leu Ala Arg Leu Phe Asn Ala Asp Gln 865 870 875 880 Ile Asp Ala Gln Thr Phe His Thr Ser Gln Pro Asn Leu Pro Pro Glu 885 890 895 Pro Phe Phe Ala Arg Thr Gln Ile Asp Arg Glu Ala Arg Arg Val Arg 900 905 910 Asp Gly Val Met Ser Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe 915 920 925 Asp Val Ala Leu Ala Thr Val Tyr Tyr Leu Asn Asp Arg Ser Val Ser 930 935 940 Gly Glu Thr Phe His Pro Ser Gly Gly Leu Arg His Glu Arg Thr Pro 945 950 955 960 Thr Gly Ala Glu Leu Tyr Gly Ser Pro Ala Pro Gln Arg Leu Ala Ser 965 970 975 Leu Ala Gly Ser Thr Val Tyr Leu Ile Gly Glu Ser Met Ala Ala His 980 985 990 Leu Glu Ala Leu Ala Arg Ala Tyr Ile Glu Arg Tyr Ala Ala Thr Arg 995 1000 1005 Val Val Leu Ile Cys Ala Thr Pro Ala Gly Val Glu Arg Phe Ser His 1010 1015 1020 His Leu Ala Asp His Leu Ala Ser Gly Ala Leu Ala Ile Leu Ser Ala 1025 1030 1035 1040 Glu Glu Gly Ile Glu Ala Ala Leu Ser Glu Ala Leu Arg Arg Phe Gly 1045 1050 1055 Pro Pro Gly Pro Val Val Ser Thr Pro Phe Gln Pro Leu Pro Ser Gln 1060 1065 1070 Pro Leu Ile Gly Arg Asn Asp Ser Asp Trp Ser Thr Val Leu Asp Val 1075 1080 1085 Ala Gly Phe Ser Ala Met Cys Glu Gln Gln Leu Thr His His Phe Arg 1090 1095 1100 Val Thr Arg Lys Leu Ser Leu Val Ala Gly Val Ser Leu Val Leu Val 1105 1110 1115 1120 Thr Pro Glu Thr Asp Ser His Ser Ser Thr Glu Gln Phe Ala Leu Ala 1125 1130 1135 Asn Phe Val Lys Thr Thr Leu His Ala Phe Thr Ala Thr Val Gly Val 1140 1145 1150 Glu Cys Glu Arg Thr Ala His Arg Ile Leu Val Asn Gln Val Asp Leu 1155 1160 1165 Gly Arg Gln Ala Arg Ala Glu Glu Pro Arg Ser Pro Ala Glu Gln Ala 1170 1175 1180 Gln Glu Met Glu Arg Phe Ile Asp Ala Ile Met Leu Thr Thr Ala Pro 1185 1190 1195 1200 Ile Pro Ala Glu Glu Asp Asn Arg Tyr Thr Gly Arg Ile Tyr Arg Gly 1205 1210 1215 Arg Ala Ile Thr Val 1220 <210> 113 <211> 1229 <212> PRT <213> HPDH-MCRrc <400> 113 Met Ser Thr Val Arg Arg Leu Glu Gly Lys Val Ala Leu Ile Thr Gly 1 5 10 15 Gly Ala Gly Asn Ile Gly Glu Val Ile Thr Arg Arg Phe Leu Ala Glu 20 25 30 Gly Ala Thr Val Val Ile Thr Gly Arg Asn Ala Glu Lys Leu Ala Val 35 40 45 Tyr Arg Arg Arg Leu Ile Asp Glu Glu Arg Val Ala Pro Glu Arg Val 50 55 60 Val Ala Leu Arg Met Asp Gly Ser Asp Ile Ala Gln Val Arg Ala Gly 65 70 75 80 Val Ala Gln Ile Val His Gly Gly Thr Asp Val Pro Ile Pro Leu His 85 90 95 Arg Ile Asp Ile Leu Val Asn Asn Ala Gly Ser Ala Gly Pro Arg Arg 100 105 110 Arg Leu Val Asp Ile Pro Leu Glu Pro Ser Glu Val Gln Pro Pro Asp 115 120 125 Ser Glu Thr Leu Ala Gln Ala Val Gly Asn Leu Val Gly Ile Thr Trp 130 135 140 Asn Leu Thr Arg Ala Ala Ala Pro His Met Pro Ser Gly Ser Ser Val 145 150 155 160 Ile Asn Ile Ser Thr Ile Phe Ser Arg Thr Asp Tyr Tyr Gly Arg Ile 165 170 175 Ala Tyr Val Ala Pro Lys Ala Ala Leu Asn Ala Leu Ser Asp Gly Leu 180 185 190 Ala Arg Glu Leu Gly Val Arg Gly Ile Arg Val Asn Thr Ile Tyr Pro 195 200 205 Gly Pro Ile Glu Ser Glu Arg Ile Tyr Thr Met Phe Gln Ala Met Asp 210 215 220 Ala Leu Lys Gly Gln Pro Glu Gly Asp Thr Ala Ser Gly Phe Leu Arg 225 230 235 240 Met Met Arg Leu Ser Arg Ile Asp Gln Asn Gly Glu Val Val Lys Arg 245 250 255 Phe Pro Ser Pro Val Asp Val Ala Asn Thr Ala Val Phe Leu Ala Ser 260 265 270 Asp Glu Ser Ala Ala Phe Thr Gly His Ala Phe Glu Val Thr His Gly 275 280 285 Met Glu Val Pro Thr Glu Ser Arg Thr Thr Phe Val Ser Arg Pro Gly 290 295 300 Leu Arg Ser Val Asp Ala Thr Gly Lys Val Ile Leu Ile Cys Ala Gly 305 310 315 320 Asp Gln Val Asp Asp Ala Val Ala Leu Ala Asp Thr Leu Arg Ser Cys 325 330 335 Arg Ala Thr Val Val Ile Gly Phe Arg Asp Pro Arg Ala Leu Glu Lys 340 345 350 Ala Ser Val Leu Leu Arg Glu Pro Arg His Ala Leu Ala Ala Asp Met 355 360 365 Tyr Gly Arg Pro Thr Met Thr Ala Glu Ala Arg Leu Val Arg Leu Asp 370 375 380 Pro Leu Asp Pro Arg Ala Ala Ala Gln Thr Leu Glu Gln Ile His Ala 385 390 395 400 Glu Leu Gly Ala Ile His His Ala Val Val Leu Pro Gly Gln Ser Arg 405 410 415 His Ala Pro Ser Ala Ser Leu Ile Glu Val Asp Asp Gln Val Val Glu 420 425 430 Arg Phe Leu His Gln Glu Leu Val Gly Thr Ile Ala Leu Ala Arg Glu 435 440 445 Leu Ala Arg Phe Trp Glu Glu Tyr Pro Ser Gly Ser Ser Met His Arg 450 455 460 Val Leu Phe Val Ser Asn Pro Asp Asp Gln Gln Gly Asn Gln Tyr Ser 465 470 475 480 His Ile Leu Arg Ala Ala Val Glu Gln Leu Val Arg Val Trp Arg His 485 490 495 Glu Ser Glu Tyr Asp Ser Val Asn Pro Ala His Gln Gln Glu Gly Gln 500 505 510 Ser Ser Ala Ala Val Trp Ala Asn Gln Leu Ile Arg Tyr Val Asn Asn 515 520 525 Glu Met Ala Asn Leu Asp Phe Thr Cys Ala Trp Val Ala Lys Leu Leu 530 535 540 Gly Ser Asp Arg Arg Ile Ala Glu Ile Asn Leu Tyr Leu Pro Glu Glu 545 550 555 560 Ile Val Gly Thr Ile Gly Val His Asn Pro Gly Phe Gly Trp Ala Glu 565 570 575 Ser Leu Phe Gly Leu His Met Gly Lys Val Ala Leu Ile Thr Gly Gly 580 585 590 Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu Leu Ala Leu Ser Gly 595 600 605 Ala His Val Met Leu Ala Ala Arg Asn Ala Asp Gln Leu Glu Gln Met 610 615 620 Arg Ala Ser Ile Val Arg Glu Val Arg Asp Ala Ser Tyr Pro Asp Ala 625 630 635 640 Glu Ser Arg Val Ala Ile Phe Pro Gly Ser Asp Val Ser Asp Ile Asp 645 650 655 Gly Leu Glu Arg Leu Val Asn His Thr Val Arg Val Phe Gly Lys Val 660 665 670 Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly Ala Glu Glu Met Val 675 680 685 Ile Asp Met Pro Val Asp Ala Trp Arg His Thr Leu Arg Ala Asn Leu 690 695 700 Ile Ser Asn Tyr Ala Leu Leu Arg Arg Leu Ala Pro Gln Met Lys Ala 705 710 715 720 Ala Gly Gly Ala Tyr Val Leu Asn Val Ser Ser Tyr Phe Gly Gly Glu 725 730 735 Lys Tyr Val Ala Ile Pro Tyr Pro Asn Arg Ser Asp Tyr Ala Val Ser 740 745 750 Lys Ala Gly Gln Arg Ala Met Val Glu Ser Leu Ala Arg Phe Leu Gly 755 760 765 Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly Pro Val Glu Gly Glu 770 775 780 Arg Leu Lys Gly Ala Gly Ser Arg Pro Gly Leu Phe Met Arg Arg Ala 785 790 795 800 Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu Val Phe Ala Ala Leu 805 810 815 Leu Ala Ala Arg His Glu Gly Ala Thr Ile Ala Asp Leu Leu Pro Asp 820 825 830 Leu Phe Ala Asn Asp Ile Gln Ser Ile Ala Asn Ser Ala Ala Met Pro 835 840 845 Ala Pro Leu Arg Arg Leu Ala Thr Met Leu Arg Glu Thr Ser Asp Ala 850 855 860 Gly Gly Ser Ala Gln Ser Tyr Leu Met Asn Ala Thr Ile Ala Arg Lys 865 870 875 880 Leu Leu Asn Arg Leu Glu Asn Gly Gly Tyr Ile Thr Leu His Asp Arg 885 890 895 Arg Ala Leu Thr Val Glu Pro Pro Glu Pro Phe Phe Thr Glu Ala Gln 900 905 910 Ile Glu Arg Glu Ala Ile Lys Val Arg Asp Gly Ile Leu Gly Met Leu 915 920 925 His Leu Gln Arg Met Pro Thr Glu Phe Asp Val Ala Leu Ala Thr Val 930 935 940 Phe Tyr Leu Ala Asp Arg Asn Val Thr Gly Glu Thr Phe His Pro Ser 945 950 955 960 Gly Gly Leu Arg Phe Glu Arg Thr Val Thr Glu Gly Glu Leu Phe Gly 965 970 975 Lys Pro Gly Gln Gln Arg Leu Glu Arg Leu Lys Gly Ser Val Val Tyr 980 985 990 Leu Ile Gly Glu His Leu Arg Gln His Leu Val Leu Leu Ala Arg Thr 995 1000 1005 Phe Leu Asp Glu Ile His Val Ala Arg Val Val Leu Leu Thr Glu Thr 1010 1015 1020 Thr Gln Ala Ala Thr Asp Leu Ala Ala Glu Leu Ser Asp Tyr Glu Ala 1025 1030 1035 1040 Ala Gly Arg Phe Val Val Ile Pro Thr Cys Gly Asp Ile Glu Gly Gly 1045 1050 1055 Ile Asp Arg Ala Met Ala Glu Tyr Gly Arg Pro Gly Pro Val Ile Ser 1060 1065 1070 Thr Pro Phe Arg Pro Leu Pro Asp Arg Ala Leu Ser Ala Arg Asn Gly 1075 1080 1085 Asp Trp Ser Ser Val Leu Thr Thr Ala Glu Phe Glu Glu Leu Val Glu 1090 1095 1100 Gln Gln Ile Thr His His Phe Arg Val Ala Arg Lys Ala Gly Leu Ile 1105 1110 1115 1120 Glu Gly Ala Asn Val Thr Leu Val Thr Pro Pro Thr Ser Ala Arg Ser 1125 1130 1135 Thr Ser Glu Glu Phe Ala Leu Ala Asn Phe Val Lys Thr Thr Leu His 1140 1145 1150 Ala Leu Thr Ala Thr Ala Gly Ala Glu Ser Glu Arg Thr Val Pro His 1155 1160 1165 Val Pro Val Asn Gln Val Asp Leu Thr Arg Arg Ala Arg Ser Glu Glu 1170 1175 1180 Pro Arg Thr Pro Ser Glu Glu Glu Glu Glu Leu Gln Arg Phe Val Asn 1185 1190 1195 1200 Ala Val Leu Leu Thr Ser Ala Pro Leu Pro Thr Pro Leu Glu Ser Arg 1205 1210 1215 Tyr Arg Ala Arg Ile Tyr Arg Gly Asn Ala Ile Thr Val 1220 1225 <210> 114 <211> 1204 <212> PRT <213> HPDH-MCRsl <400> 114 Met Asn Ala Met Gly Lys Thr Glu Thr Gln Gly Arg Leu Ala Gly Lys 1 5 10 15 Val Ala Leu Ile Thr Gly Ala Ala Gly Asn Leu Gly Gly Ser Ile Val 20 25 30 Arg Arg Tyr Leu Ala Glu Gly Ala Thr Val Val Met Ser Gly Arg Asp 35 40 45 Ala Ala Arg Thr Gln Ala Ala Leu Asp Ala Ala Val Ala Asp Val Gly 50 55 60 Gly Thr Ala Ser Ile Val Thr Met Asp Gly Ala Asn Ile Glu Ser Val 65 70 75 80 Arg Ala Gly Ile Ala Asp Val Val Ala Arg His Gly Arg Ile Asp Val 85 90 95 Leu Val Asn Asn Ala Gly Ser Ala Gly Pro Lys Gln Val Leu Gly Asp 100 105 110 Leu Pro Leu Leu Pro Glu Glu Leu Glu Gly Thr Pro Asp Ser Glu Thr 115 120 125 Val Gly Asp Ala Ala Arg Asn Ile Met Val Val Ala Trp Asn Leu Val 130 135 140 Arg Ala Ala Ala Ser Ala Met Gly Glu Gly Gly Ser Ile Ile Asn Val 145 150 155 160 Ser Thr Ile Phe Ser Arg Thr Asp Tyr Phe Gly Arg Ala Ala Tyr Thr 165 170 175 Val Pro Lys Ala Ala Leu Asn Ile Leu Ser Lys Gly Ile Ala Arg Glu 180 185 190 Leu Gly Thr Arg Gly Ile Arg Val Asn Gln Leu Tyr Pro Gly Pro Ile 195 200 205 Ala Ser Glu Arg Ile Arg Thr Val Phe Ala Ala Met Asp Lys Leu Lys 210 215 220 Gly Asp Asp Ala Gly Ser Thr Ala Asn His Phe Phe Asn Leu Met Ala 225 230 235 240 Leu Glu Arg Ser Val Gly Glu Asp Ala Lys Ala Lys Thr Phe Pro Thr 245 250 255 Pro Ala Asp Ile Ala Asn Thr Cys Val Phe Leu Gly Ser Asp Glu Ser 260 265 270 Ala Ala Phe Ala Ala His Asp Phe Glu Val Thr His Gly Met Ala Val 275 280 285 Pro Leu Glu Ser Lys Ser Glu Phe Leu His Arg Pro Asp Leu Arg Ala 290 295 300 Ile Asp Gly Asp Gly His Gly Ile Leu Ile Ser Ala Gly Asn Gln Leu 305 310 315 320 Asp Asp Ala Leu Ala Met Ala Gly Leu Phe Ala Ala Gln Gly Ser Lys 325 330 335 Val Leu Leu Gly Phe His Ala Gln Ala Ala Leu Asp Asp Ala Ala Glu 340 345 350 Arg Leu Pro Ala Gly Val Lys Ala Val Arg Phe Pro Arg His Asp His 355 360 365 Met Ala Met Asp Ala Glu Leu Ala Arg Phe Thr Ala His Val Ala Ala 370 375 380 Ile Asp Gly Ala Ile Ile Leu Pro Thr Arg Gly Pro Gly Tyr Phe His 385 390 395 400 Gly Ala Leu Asn Glu Ala Ser Asp Ala Ala Val Glu Arg Phe Val Asp 405 410 415 Asp Glu Leu Glu Gly Ala Ile Ala Val Ala Arg Thr Leu Asp Arg Tyr 420 425 430 Trp Gln Gln Gly Gln Asp Leu Pro Gly Asp Pro Arg Val Leu Phe Ile 435 440 445 Thr Asn Pro Asp Asp Gly Ala Gly Asn Gly Trp Ala Asp Val Gly Arg 450 455 460 Ala Ala Ile Glu Glu Leu Leu Arg Val Trp Arg Asp Glu Ala Arg Val 465 470 475 480 Ala Ala Thr Gln Gly Lys Thr Pro Arg Pro Val Trp Gly Asn Gln Ile 485 490 495 Val Arg Phe Gly Asn Asn Glu Pro Glu Asn Arg Asp Phe Ala Ala Gly 500 505 510 His Ala Ala Arg Leu Leu Leu Gly Gly Arg Gln Ile Glu Pro Ile Asn 515 520 525 Leu Tyr Ile Pro Ala Ser Ile Thr Lys Ala Thr Gly Ala Arg Arg Ala 530 535 540 Leu Ala Gly Thr Ala Glu Asn Ile Thr Gly Leu His Leu Gly Lys Val 545 550 555 560 Ala Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Ala Leu Ala Arg 565 570 575 Leu Leu Ala Leu Ala Gly Ala Lys Val Met Met Val Ala Arg Arg Glu 580 585 590 Ser Glu Leu Asp Ala Ala Arg Glu Arg Ile Val Gly Glu Leu Gln Asp 595 600 605 Ile Gly Phe Ala Gly Val Glu Arg Arg Val Leu Thr Leu Ala Gly Ile 610 615 620 Asp Val Ala Asp Met Lys Ser Leu Lys His Ala Val Asp Glu Thr Val 625 630 635 640 Arg Ala Phe Gly Arg Ile Asp Tyr Leu Ile Asn Asn Ala Gly Val Ser 645 650 655 Gly Ala Glu Glu Met Ala Val Asp Met Gly Val Glu Asn Phe Arg His 660 665 670 Thr Gln Phe Ala Asn Leu Ile Ser Asn Tyr Ala Leu Met His Met Val 675 680 685 Val Pro Met Met Arg Lys Gln Gly Ser Gly Tyr Ile Leu Asn Val Ser 690 695 700 Ser Tyr Phe Gly Gly Glu Lys Tyr Leu Ala Val Ser Tyr Pro Asn Arg 705 710 715 720 Thr Asp Tyr Ala Val Ser Lys Ser Gly Gln Arg Ala Met Val Glu Ser 725 730 735 Met Ala Arg His Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro 740 745 750 Gly Pro Val Asp Gly Asp Arg Leu Ala Gly Leu Gly Gly Lys Pro Gly 755 760 765 Leu Phe Glu Arg Arg Gly Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn 770 775 780 Ala Val His Ser Ala Val Ile Lys Ser Val Arg Ala Gly Gly Ser Val 785 790 795 800 Ala Gln Ile Leu Phe Arg Leu Ser Arg Asn Asp Ser Val Gln Leu Ser 805 810 815 His Asp Lys Ser Asn Pro Gln Pro Leu Arg Asp Leu Ala Leu Ala Cys 820 825 830 Ala Arg Glu Gly Asp Glu Ser Cys Cys Trp Asp Arg Phe Ile Leu Thr 835 840 845 Pro Val Leu Ala Ala Lys Leu Leu Lys Arg Leu Arg Leu Gly Gly Trp 850 855 860 Leu Ile Gly Thr Asp Trp Gln Ser Arg Asp Glu Ser Asp Trp Leu Leu 865 870 875 880 Arg Val Pro Pro Asp Asp Met Pro Trp Leu Pro Val Ala Gln Ile Gly 885 890 895 Arg Glu Ala Ala Lys Val Arg Gly Gly Val Leu Ser Gln Leu His Leu 900 905 910 Gly Lys Met Pro Thr Glu Thr Glu Val Ala Gln Ala Thr Val Phe Phe 915 920 925 Leu Ala Asp Arg Ala Val Ser Gly Glu Thr Phe Met Pro Ser Gly Gly 930 935 940 Leu Asn Val Glu Arg Ser Ala Thr Glu Arg Glu Leu Phe Gly Ser Pro 945 950 955 960 Lys Ala Glu Arg Leu Ala Gln Met Gly Gly Lys Thr Val Trp Leu Val 965 970 975 Gly Glu His Leu Ala Asp Tyr Leu Gly Ala Ala Ala Gln Gly Phe Leu 980 985 990 Asn Cys Asp Val Ala Arg Val Val Leu Ile Thr Ala Ser Ala Ala Gly 995 1000 1005 Ala Thr Ala Ile Lys Gly His Ile Gly Ser Gly Ala Asp Arg Val Glu 1010 1015 1020 Ser Leu Leu Ile Asp Gly Glu Ile Glu Thr Ala Leu Asp Ala Ala Leu 1025 1030 1035 1040 Ala Thr Trp Gly His Pro Thr Thr Ile Val Ser Thr Pro Leu Lys Ala 1045 1050 1055 Leu Pro Asp Gln Leu Phe Asp Ala Asp Asn Pro Leu Asp Gly Asp Gly 1060 1065 1070 Phe Ala Asp Val Val Glu His Asn Leu Thr His His Phe Arg Ile Ala 1075 1080 1085 Arg Lys Val Ser Leu Phe Asp Glu Cys Gln Leu Val Leu Val Ser Pro 1090 1095 1100 Asp Val Pro Met Gly Glu Gly Ala Lys Gly Ala Phe Ala Leu Ala Asn 1105 1110 1115 1120 Phe Val Lys Thr Thr Leu His Ala Phe Thr Ala Thr Ile Ala Val Glu 1125 1130 1135 Asn Glu Arg Leu Val His Glu Val Ala Val Asn Gln Ile Asn Leu Thr 1140 1145 1150 Arg Arg Val Arg Ser Glu Glu Pro Lys Asn Ala Ala Glu His Glu Glu 1155 1160 1165 Glu Val Arg Arg Phe Ala Arg Ala Val Leu Leu Ala Gly Ala Pro Leu 1170 1175 1180 Pro Asp Ala Glu Asp Ser Arg Tyr Arg Ser Arg Ile Tyr Arg Gly Met 1185 1190 1195 1200 Ser Leu Thr Val <210> 115 <211> 1220 <212> PRT <213> HPDH-MCRca_(Variant_3) <400> 115 Met Ser Gly Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly 1 5 10 15 Gly Ala Gly Asn Ile Gly Ser Glu Leu Thr Arg Arg Phe Leu Ala Glu 20 25 30 Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Arg Ala Lys Leu Thr Ala 35 40 45 Leu Ala Glu Arg Met Gln Ala Glu Ala Gly Val Pro Ala Lys Arg Ile 50 55 60 Asp Leu Glu Val Met Asp Gly Ser Asp Pro Val Ala Val Arg Ala Gly 65 70 75 80 Ile Glu Ala Ile Val Ala Arg His Gly Gln Ile Asp Ile Leu Val Asn 85 90 95 Asn Ala Gly Ser Ala Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu 100 105 110 Thr Glu Ala Glu Leu Gly Pro Gly Ala Glu Glu Thr Leu His Ala Ser 115 120 125 Ile Ala Asn Leu Leu Gly Met Gly Trp His Leu Met Arg Ile Ala Ala 130 135 140 Pro His Met Pro Val Gly Ser Ala Val Ile Asn Val Ser Thr Ile Phe 145 150 155 160 Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Thr Pro Lys Ala 165 170 175 Ala Leu Asn Ala Leu Ser Gln Leu Ala Ala Arg Glu Leu Gly Ala Arg 180 185 190 Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Asp Arg 195 200 205 Ile Arg Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu 210 215 220 Gly Asp Thr Ala His His Phe Leu Asn Thr Met Arg Leu Cys Arg Ala 225 230 235 240 Asn Asp Gln Gly Ala Leu Glu Arg Arg Phe Pro Ser Val Gly Asp Val 245 250 255 Ala Asp Ala Ala Val Phe Leu Ala Ser Ala Glu Ser Ala Ala Leu Ser 260 265 270 Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Ala Cys Ser 275 280 285 Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Ser 290 295 300 Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met 305 310 315 320 Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly 325 330 335 Phe Arg Ser Ala Ala Ala Leu Ala Gln Phe Glu Gln Ala Val Asn Glu 340 345 350 Ser Arg Arg Leu Ala Gly Ala Asp Phe Thr Pro Pro Ile Ala Leu Pro 355 360 365 Leu Asp Pro Arg Asp Pro Ala Thr Ile Asp Ala Val Phe Asp Trp Gly 370 375 380 Ala Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala 385 390 395 400 Thr Ser His Glu Pro Ala Pro Cys Val Ile Glu Val Asp Asp Glu Arg 405 410 415 Val Leu Asn Phe Leu Ala Asp Glu Ile Thr Gly Thr Ile Val Ile Ala 420 425 430 Ser Arg Leu Ala Arg Tyr Trp Gln Ser Gln Arg Leu Thr Pro Gly Ala 435 440 445 Arg Ala Arg Gly Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Asp Gln 450 455 460 Asn Gly Asn Val Tyr Gly Arg Ile Gln Ser Ala Ala Ile Gly Gln Leu 465 470 475 480 Ile Arg Val Trp Arg His Glu Ala Glu Leu Asp Tyr Gln Arg Ala Ser 485 490 495 Ala Ala Gly Asp His Val Leu Pro Pro Val Trp Ala Asn Gln Ile Val 500 505 510 Arg Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp 515 520 525 Thr Ala Gln Leu Leu His Ser Gln Arg His Ile Asn Glu Ile Thr Leu 530 535 540 Asn Ile Pro Ala Asn Ile Ser Ala Thr Thr Gly Ala Arg Ser Ala Ser 545 550 555 560 Val Gly Trp Ala Glu Ser Leu Ile Gly Leu His Leu Gly Lys Val Ala 565 570 575 Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu 580 585 590 Leu Ala Leu Ser Gly Ala Arg Val Met Leu Ala Asp Ile Gln Glu Asp 595 600 605 Lys Leu Glu Gln Met Gln Ala Met Ile Gln Ser Glu Leu Ala Glu Val 610 615 620 Gly Tyr Thr Asp Val Glu Asp Arg Val His Ile Ala Pro Gly Cys Asp 625 630 635 640 Val Ser Ser Glu Ala Gln Leu Ala Asp Leu Val Glu Arg Thr Leu Ser 645 650 655 Ala Phe Gly Thr Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly 660 665 670 Val Glu Glu Met Val Ile Asp Met Pro Val Glu Gly Trp Arg His Thr 675 680 685 Leu Phe Ala Asn Leu Ile Ser Asn Tyr Ser Leu Met Arg Lys Leu Ala 690 695 700 Pro Leu Met Lys Lys Gln Gly Ser Gly Tyr Ile Leu Asn Val Ser Ser 705 710 715 720 Tyr Phe Gly Gly Glu Lys Asp Ala Ala Ile Pro Tyr Pro Asn Arg Ala 725 730 735 Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Ala Glu Val Phe 740 745 750 Ala Arg Phe Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly 755 760 765 Pro Val Glu Gly Asp Arg Leu Arg Gly Thr Gly Glu Arg Pro Gly Leu 770 775 780 Phe Ala Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu 785 790 795 800 Leu His Ala Ala Leu Ile Ala Ala Ala Arg Thr Asp Glu Arg Ser Met 805 810 815 His Glu Leu Val Glu Leu Leu Leu Pro Asn Asp Val Ala Ala Leu Glu 820 825 830 Gln Asn Pro Ala Ala Pro Thr Ala Leu Arg Glu Leu Ala Arg Arg Phe 835 840 845 Arg Ser Glu Gly Asp Pro Ala Ala Ser Ser Ser Ser Ala Leu Leu Asn 850 855 860 Arg Ser Ile Ala Ala Lys Leu Leu Ala Arg Leu His Asn Gly Gly Tyr 865 870 875 880 Val Leu Pro Ala Asp Ile Phe Ala Asn Leu Pro Asn Pro Pro Asp Pro 885 890 895 Phe Phe Thr Arg Ala Gln Ile Asp Arg Glu Ala Arg Lys Val Arg Asp 900 905 910 Gly Ile Met Gly Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe Asp 915 920 925 Val Ala Met Ala Thr Val Tyr Tyr Leu Ala Asp Arg Asn Val Ser Gly 930 935 940 Glu Thr Phe His Pro Ser Gly Gly Leu Arg Tyr Glu Arg Thr Pro Thr 945 950 955 960 Gly Gly Glu Leu Phe Gly Leu Pro Ser Pro Glu Arg Leu Ala Glu Leu 965 970 975 Val Gly Ser Thr Val Tyr Leu Ile Gly Glu His Leu Thr Glu His Leu 980 985 990 Asn Leu Leu Ala Arg Ala Tyr Leu Glu Arg Tyr Gly Ala Arg Gln Val 995 1000 1005 Val Met Ile Val Glu Thr Glu Thr Gly Ala Glu Thr Met Arg Arg Leu 1010 1015 1020 Leu His Asp His Val Glu Ala Gly Arg Leu Met Thr Ile Val Ala Gly 1025 1030 1035 1040 Asp Gln Ile Glu Ala Ala Ile Asp Gln Ala Ile Thr Arg Tyr Gly Arg 1045 1050 1055 Pro Gly Pro Val Val Cys Thr Pro Phe Arg Pro Leu Pro Thr Val Pro 1060 1065 1070 Leu Val Gly Arg Lys Asp Ser Asp Trp Ser Thr Val Leu Ser Glu Ala 1075 1080 1085 Glu Phe Ala Glu Leu Cys Glu His Gln Leu Thr His His Phe Arg Val 1090 1095 1100 Ala Arg Lys Ile Ala Leu Ser Asp Gly Ala Ser Leu Ala Leu Val Thr 1105 1110 1115 1120 Pro Glu Thr Thr Ala Thr Ser Thr Thr Glu Gln Phe Ala Leu Ala Asn 1125 1130 1135 Phe Ile Lys Thr Thr Leu His Ala Phe Thr Ala Thr Ile Gly Val Glu 1140 1145 1150 Ser Glu Arg Thr Ala Gln Arg Ile Leu Ile Asn Gln Val Asp Leu Thr 1155 1160 1165 Arg Arg Ala Arg Ala Glu Glu Pro Arg Asp Pro His Glu Arg Gln Gln 1170 1175 1180 Glu Leu Glu Arg Phe Ile Glu Ala Val Leu Leu Val Thr Ala Pro Leu 1185 1190 1195 1200 Pro Pro Glu Ala Asp Thr Arg Tyr Ala Gly Arg Ile His Arg Gly Arg 1205 1210 1215 Ala Ile Thr Val 1220 <210> 116 <211> 1220 <212> PRT <213> HPDH-MCRca_(Variant_6) <400> 116 Met Ser Gly Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly 1 5 10 15 Gly Ala Gly Asn Ile Gly Ser Glu Leu Thr Arg Arg Phe Leu Ala Glu 20 25 30 Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Arg Ala Lys Leu Thr Ala 35 40 45 Leu Ala Glu Arg Met Gln Ala Glu Ala Gly Val Pro Ala Lys Arg Ile 50 55 60 Asp Leu Glu Val Met Asp Gly Ser Asp Pro Val Ala Val Arg Ala Gly 65 70 75 80 Ile Glu Ala Ile Val Ala Arg His Gly Gln Ile Asp Ile Leu Val Asn 85 90 95 Asn Ala Gly Ser Ala Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu 100 105 110 Thr Glu Ala Glu Leu Gly Pro Gly Ala Glu Glu Thr Leu His Ala Ser 115 120 125 Ile Ala Asn Leu Leu Gly Met Gly Trp His Leu Met Arg Ile Ala Ala 130 135 140 Pro His Met Pro Val Gly Ser Ala Val Ile Asn Val Ser Thr Ile Phe 145 150 155 160 Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Thr Pro Lys Ala 165 170 175 Ala Leu Asn Ala Leu Ser Gln Leu Ala Ala Arg Glu Leu Gly Ala Arg 180 185 190 Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Asp Arg 195 200 205 Ile Arg Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu 210 215 220 Gly Asp Thr Ala His His Phe Leu Asn Thr Met Arg Leu Cys Arg Ala 225 230 235 240 Asn Asp Gln Gly Ala Leu Glu Arg Arg Phe Pro Ser Val Gly Asp Val 245 250 255 Ala Asp Ala Ala Val Phe Leu Ala Ser Ala Glu Ser Ala Ala Leu Ser 260 265 270 Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Ala Cys Ser 275 280 285 Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Ser 290 295 300 Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met 305 310 315 320 Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly 325 330 335 Phe Arg Ser Ala Ala Ala Leu Ala Gln Phe Glu Gln Ala Val Asn Glu 340 345 350 Ser Arg Arg Leu Ala Gly Ala Asp Phe Thr Pro Pro Ile Ala Leu Pro 355 360 365 Leu Asp Pro Arg Asp Pro Ala Thr Ile Asp Ala Val Phe Asp Trp Gly 370 375 380 Ala Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala 385 390 395 400 Thr Ser His Glu Pro Ala Pro Cys Val Ile Glu Val Asp Asp Glu Arg 405 410 415 Val Leu Asn Phe Leu Ala Asp Glu Ile Thr Gly Thr Ile Val Ile Ala 420 425 430 Ser Arg Leu Ala Arg Tyr Trp Gln Ser Gln Arg Leu Thr Pro Gly Ala 435 440 445 Arg Ala Arg Gly Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Asp Gln 450 455 460 Asn Gly Asn Val Tyr Gly Arg Ile Gln Ser Ala Ala Ile Gly Gln Leu 465 470 475 480 Ile Arg Val Trp Arg His Glu Ala Glu Leu Asp Tyr Gln Arg Ala Ser 485 490 495 Ala Ala Gly Asp His Val Leu Pro Pro Val Trp Ala Asn Gln Ile Val 500 505 510 Arg Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp 515 520 525 Thr Ala Gln Leu Leu His Ser Gln Arg His Ile Asn Glu Ile Thr Leu 530 535 540 Asn Ile Pro Ala Asn Ile Ser Ala Thr Thr Gly Ala Arg Ser Ala Ser 545 550 555 560 Val Gly Trp Ala Glu Ser Leu Ile Gly Leu His Leu Gly Lys Val Ala 565 570 575 Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu 580 585 590 Leu Ala Leu Ser Gly Ala Arg Val Met Leu Ala Asp Val Asp Thr Arg 595 600 605 Lys Leu Glu Gln Met Gln Ala Met Ile Gln Ser Glu Leu Ala Glu Val 610 615 620 Gly Tyr Thr Asp Val Glu Asp Arg Val His Ile Ala Pro Gly Cys Asp 625 630 635 640 Val Ser Ser Glu Ala Gln Leu Ala Asp Leu Val Glu Arg Thr Leu Ser 645 650 655 Ala Phe Gly Thr Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly 660 665 670 Val Glu Glu Met Val Ile Asp Met Pro Val Glu Gly Trp Arg His Thr 675 680 685 Leu Phe Ala Asn Leu Ile Ser Asn Tyr Ser Leu Met Arg Lys Leu Ala 690 695 700 Pro Leu Met Lys Lys Gln Gly Ser Gly Tyr Ile Leu Asn Val Ser Ser 705 710 715 720 Tyr Phe Gly Gly Glu Lys Asp Ala Ala Ile Pro Tyr Pro Asn Arg Ala 725 730 735 Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Ala Glu Val Phe 740 745 750 Ala Arg Phe Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly 755 760 765 Pro Val Glu Gly Asp Arg Leu Arg Gly Thr Gly Glu Arg Gly Gly Leu 770 775 780 Phe Gly Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu 785 790 795 800 Leu His Ala Ala Leu Ile Ala Ala Ala Arg Thr Asp Glu Arg Ser Met 805 810 815 His Glu Leu Val Glu Leu Leu Leu Pro Asn Asp Val Ala Ala Leu Glu 820 825 830 Gln Asn Pro Ala Ala Pro Thr Ala Leu Arg Glu Leu Ala Arg Arg Phe 835 840 845 Arg Ser Glu Gly Asp Pro Ala Ala Ser Ser Ser Ser Ala Leu Leu Asn 850 855 860 Arg Ser Ile Ala Ala Lys Leu Leu Ala Arg Leu His Asn Gly Gly Tyr 865 870 875 880 Val Leu Pro Ala Asp Ile Phe Ala Asn Leu Pro Asn Pro Pro Asp Pro 885 890 895 Phe Phe Thr Arg Ala Gln Ile Asp Arg Glu Ala Arg Lys Val Arg Asp 900 905 910 Gly Ile Met Gly Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe Asp 915 920 925 Val Ala Met Ala Thr Val Tyr Tyr Leu Ala Asp Arg Asn Val Ser Gly 930 935 940 Glu Thr Phe His Pro Ser Gly Gly Leu Arg Tyr Glu Arg Thr Pro Thr 945 950 955 960 Gly Gly Glu Leu Phe Gly Leu Pro Ser Pro Glu Arg Leu Ala Glu Leu 965 970 975 Val Gly Ser Thr Val Tyr Leu Ile Gly Glu His Leu Thr Glu His Leu 980 985 990 Asn Leu Leu Ala Arg Ala Tyr Leu Glu Arg Tyr Gly Ala Arg Gln Val 995 1000 1005 Val Met Ile Val Glu Thr Glu Thr Gly Ala Glu Thr Met Arg Arg Leu 1010 1015 1020 Leu His Asp His Val Glu Ala Gly Arg Leu Met Thr Ile Val Ala Gly 1025 1030 1035 1040 Asp Gln Ile Glu Ala Ala Ile Asp Gln Ala Ile Thr Arg Tyr Gly Arg 1045 1050 1055 Pro Gly Pro Val Val Cys Thr Pro Phe Arg Pro Leu Pro Thr Val Pro 1060 1065 1070 Leu Val Gly Arg Lys Asp Ser Asp Trp Ser Thr Val Leu Ser Glu Ala 1075 1080 1085 Glu Phe Ala Glu Leu Cys Glu His Gln Leu Thr His His Phe Arg Val 1090 1095 1100 Ala Arg Lys Ile Ala Leu Ser Asp Gly Ala Ser Leu Ala Leu Val Thr 1105 1110 1115 1120 Pro Glu Thr Thr Ala Thr Ser Thr Thr Glu Gln Phe Ala Leu Ala Asn 1125 1130 1135 Phe Ile Lys Thr Thr Leu His Ala Phe Thr Ala Thr Ile Gly Val Glu 1140 1145 1150 Ser Glu Arg Thr Ala Gln Arg Ile Leu Ile Asn Gln Val Asp Leu Thr 1155 1160 1165 Arg Arg Ala Arg Ala Glu Glu Pro Arg Asp Pro His Glu Arg Gln Gln 1170 1175 1180 Glu Leu Glu Arg Phe Ile Glu Ala Val Leu Leu Val Thr Ala Pro Leu 1185 1190 1195 1200 Pro Pro Glu Ala Asp Thr Arg Tyr Ala Gly Arg Ile His Arg Gly Arg 1205 1210 1215 Ala Ile Thr Val 1220 <210> 117 <211> 258 <212> PRT <213> BDHcm <400> 117 Met Val Asn Leu Gln Gly Lys Thr Ala Leu Val Thr Gly Ser Thr Ser 1 5 10 15 Gly Ile Gly Leu Gly Ile Ala Glu Ala Leu Gly Arg Ala Gly Ala Asn 20 25 30 Ile Val Leu Asn Gly Phe Gly Asp Val Asp Ala Ala Leu Ala Thr Ile 35 40 45 Ala Ala Thr Gly Ala Gln Ser Ala His His Pro Ala Asp Met Arg Lys 50 55 60 Pro Asp Glu Ile Glu Ala Met Ile Ala Met Ala Arg Glu Arg Phe Gly 65 70 75 80 Thr Val Asp Val Leu Val Asn Asn Ala Gly Ile Gln His Val Ala Gln 85 90 95 Val Glu Asp Phe Pro Ala Gln Lys Trp Asp Asp Ile Leu Ala Ile Asn 100 105 110 Leu Thr Ser Ser Phe His Thr Thr Arg His Val Ile Pro Ala Met Arg 115 120 125 Ala Arg Asn Trp Gly Arg Ile Val Asn Ile Ala Ser Val His Gly Leu 130 135 140 Val Gly Ser Ala Gly Lys Ser Ala Tyr Val Ala Ala Lys His Gly Leu 145 150 155 160 Val Gly Leu Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Thr Gly Ile 165 170 175 Thr Cys Asn Ala Ile Cys Pro Gly Phe Val Leu Thr Pro Leu Val Ala 180 185 190 Ala Gln Ile Asp Ala Ile Ala Lys Arg Asp Gly Ile Ser Met Glu Ala 195 200 205 Ala Arg Ala Arg Leu Leu Ser Asp Lys Gln Pro Ser Gly Gln Phe Val 210 215 220 Thr Pro Glu Gln Leu Gly Asn Leu Val Leu Met Leu Cys Ser Pro Phe 225 230 235 240 Gly Asp Gln Val Arg Gly Ala Ala Trp Ala Met Asp Gly Gly Trp Thr 245 250 255 Ala Gln <210> 118 <211> 256 <212> PRT <213> BDHkp <400> 118 Met Asn Leu His Gly Lys Thr Ala Leu Val Thr Gly Ser Thr Ser Gly 1 5 10 15 Ile Gly Leu Gly Ile Ala Lys Val Leu Ala Gln Ala Gly Ala Gln Leu 20 25 30 Val Leu Asn Gly Phe Gly Asp Ser Ser His Ala Arg Ala Glu Val Ala 35 40 45 Ala Leu Gly Lys Ile Pro Gly Tyr His Asp Ala Asp Leu Arg Asp Val 50 55 60 Gly Gln Ile Glu Ala Met Met His Tyr Ala Glu Ser Thr Phe Gly Gly 65 70 75 80 Val Asp Ile Val Ile Asn Asn Ala Gly Ile Gln His Val Ala Pro Val 85 90 95 Glu Gln Phe Pro Val Asp Lys Trp Asn Asp Ile Leu Ala Ile Asn Leu 100 105 110 Ser Ser Val Phe His Thr Thr Arg Leu Ala Leu Pro Gly Met Arg Gln 115 120 125 Arg Asn Trp Gly Arg Ile Ile Asn Ile Ala Ser Val His Gly Leu Val 130 135 140 Ala Ser Lys Glu Lys Ser Ala Tyr Val Ala Ala Lys His Ala Val Val 145 150 155 160 Gly Leu Thr Lys Thr Val Ala Leu Glu Thr Ala Arg Ser Gly Ile Thr 165 170 175 Cys Asn Ala Ile Cys Pro Gly Trp Val Leu Thr Pro Leu Val Gln Gln 180 185 190 Gln Ile Asp Lys Arg Ile Ala Glu Gly Val Asp Pro Glu Gln Ala Ser 195 200 205 Ala Gln Leu Leu Ala Glu Lys Gln Pro Ser Gly Glu Phe Val Thr Pro 210 215 220 Gln Gln Leu Gly Glu Met Ala Leu Phe Leu Cys Ser Asp Ala Ala Ala 225 230 235 240 Gln Val Arg Gly Ala Ala Trp Asn Met Asp Gly Gly Trp Val Ala Gln 245 250 255 <210> 119 <211> 370 <212> PRT <213> HBDHos <400> 119 Met Gln Leu Phe Lys Leu Lys Thr Arg Leu Ser Lys Phe Asp His Phe 1 5 10 15 Ala Asp Phe Ala Arg Glu Phe Lys Leu Ser Glu His Asp Leu Val Ile 20 25 30 Thr Asn Glu Phe Leu Tyr Glu Pro Phe Met Lys Ser Leu Asn Leu Pro 35 40 45 Cys His Phe Ile Met Gln Glu Lys Phe Gly Leu Gly Glu Pro Ser Asp 50 55 60 Lys Met Met Asn Thr Ile Leu Lys Glu Val Lys Gly Val Gly Phe Asp 65 70 75 80 Arg Val Ile Ala Val Gly Gly Gly Thr Val Ile Asp Ile Ser Lys Leu 85 90 95 Phe Val Leu Lys Gly Val Glu Asp Val Thr Glu Ala Phe Glu Arg Lys 100 105 110 Ile Pro Ile Val Lys Glu Lys Gln Leu Val Ile Leu Pro Thr Thr Cys 115 120 125 Gly Thr Gly Ser Glu Val Thr Asn Ile Ser Ile Ala Glu Ile Thr Ser 130 135 140 Lys His Thr Lys Met Gly Leu Ala Asp Asp Ala Ile Val Ala Asp Asp 145 150 155 160 Ala Val Leu Val Pro Glu Leu Leu Lys Gly Leu Pro Phe Lys Phe Tyr 165 170 175 Ala Cys Ser Ala Ile Asp Ala Leu Val His Ala Thr Glu Ser Tyr Val 180 185 190 Ser Pro Lys Ser Asn Pro Tyr Thr Arg Leu Tyr Cys Arg Ala Ala Ile 195 200 205 Glu Ile Ile Ile Asp Val Phe Gln Gly Ile Ala Ala Asn Gly Pro Glu 210 215 220 Tyr Arg Phe Glu Arg Leu Gly Asp Met Leu Met Ala Ser Asn Tyr Ala 225 230 235 240 Gly Ile Ala Phe Gly Asn Thr Gly Val Gly Ala Val His Ala Leu Ser 245 250 255 Tyr Pro Leu Gly Gly Ser Tyr His Val Pro His Gly Glu Ser Asn Tyr 260 265 270 Gln Phe Phe Thr Glu Val Phe Lys Leu Tyr Asn Ala Lys Asn Pro Ser 275 280 285 Gly Asp Ile Ala Arg Leu Asn Asp Leu Leu Cys Val Gln Leu Gly Val 290 295 300 Asn Glu Asn Pro Tyr Pro Val Leu Asp Glu Leu Leu Gly Lys Leu Ile 305 310 315 320 Pro Lys Asn Lys Leu Arg Val Tyr Gly Met Lys Pro Glu Glu Ile Gln 325 330 335 Gly Phe Thr Ser Ser Val Leu Gln Thr Gln Gln Arg Leu Leu Ala Asn 340 345 350 Asn Tyr Val Ser Leu Ser Glu Glu Glu Ile Arg Glu Ile Tyr Lys Val 355 360 365 Leu Tyr 370 <210> 120 <211> 378 <212> PRT <213> HBDHps <400> 120 Met Ala Phe Ile Tyr Tyr Val Thr Gln Ile Gln Phe Glu Phe Gly Ala 1 5 10 15 Val Lys Leu Leu Lys Gln Glu Cys Glu Arg Val Gly Ile Thr Arg Pro 20 25 30 Leu Ile Val Thr Asp Pro Gly Val Lys Ala Ala Gly Ile Leu Gln Lys 35 40 45 Ala Leu Asp Ala Leu Pro Gly Ile Ala Val Thr Val Phe Asp Gln Thr 50 55 60 Pro Ser Asn Pro Thr Glu Ala Ala Val Arg Ala Ala Ala Ala Leu Tyr 65 70 75 80 Lys Ala Gly Gly Cys Asp Gly Leu Ile Ala Val Gly Gly Gly Ser Ala 85 90 95 Ile Asp Cys Ala Lys Gly Val Ala Ile Ala Ala Thr His Glu Gly Pro 100 105 110 Leu Thr Thr Tyr Ala Thr Ile Glu Gly Gly Ser Pro Lys Ile Ser Asp 115 120 125 Arg Val Ala Pro Leu Ile Ala Val Pro Thr Thr Ser Gly Thr Gly Ser 130 135 140 Glu Val Ala Arg Gly Ala Ile Ile Ile Val Asp Asp His Arg Lys Leu 145 150 155 160 Gly Phe His Ser Trp His Leu Val Pro Lys Thr Ala Ile Cys Asp Pro 165 170 175 Glu Leu Thr Leu Gly Leu Pro Ala Met Leu Thr Ala Ala Thr Gly Met 180 185 190 Asp Ala Ile Ala His Cys Met Glu Thr Phe Met Ser Pro Ala Phe Asn 195 200 205 Pro Pro Ala Asp Gly Ile Gly Leu Asp Gly Leu Glu Arg Gly Trp Ser 210 215 220 His Ile Glu Arg Ala Thr Arg Asp Gly Ser Asp Arg Glu Ala Arg Leu 225 230 235 240 Asn Leu Met Ser Ala Ser Met Gln Gly Ala Met Ala Phe Gln Lys Gly 245 250 255 Leu Gly Cys Val His Ser Leu Ser His Ser Leu Gly Gly Val Asp Pro 260 265 270 Arg Leu His His Gly Thr Leu Asn Ala Met Phe Leu Pro Ala Val Val 275 280 285 Arg Phe Asn Ala Ser Ala Glu Ser Val Gln Lys Glu Arg Arg Leu Asp 290 295 300 Arg Met Ala Arg Ala Met Gly Leu Arg Ser Gly Ser Asp Ile Pro Asp 305 310 315 320 Ala Ile Lys Asp Met Asn Ala Arg Leu Gly Leu Pro Thr Gly Leu Ala 325 330 335 Ala Met Gly Val Gln Arg Asp Trp Phe Asp Arg Ile Ile Val Gly Ala 340 345 350 Leu Ala Asp His Cys His Lys Thr Asn Pro Arg Leu Ala Ser Pro Glu 355 360 365 Glu Tyr Arg Glu Met Leu Glu Thr Ser Leu 370 375 <210> 121 <211> 288 <212> PRT <213> HIBADHas <400> 121 Met Gln Thr Ile Gly Phe Ile Gly Leu Gly Val Met Gly Lys Ser Met 1 5 10 15 Ala Arg His Leu Leu Arg Ala Gly Tyr Ser Leu Val Val Tyr Thr Arg 20 25 30 Thr Lys Gln Lys Ala Glu Asp Ile Leu Ala Glu Gly Ala Leu Trp Lys 35 40 45 Glu Thr Val Lys Asp Val Ala Lys Ala Ala Asp Val Val Met Thr Met 50 55 60 Val Gly Glu Pro Gln Asp Val Glu Tyr Val Tyr Phe Gly Asp Asp Gly 65 70 75 80 Ile Leu Ala His Ala Lys Arg Gly Thr Tyr Val Ile Asp Phe Thr Thr 85 90 95 Ser Lys Pro Ser Leu Ala Val Arg Ile His Glu Ala Ala Lys Glu Lys 100 105 110 Gly Ile Phe Val Leu Asp Ala Pro Val Ser Gly Gly Asp Ile Gly Ala 115 120 125 Arg Asp Gly Thr Leu Ser Ile Met Val Gly Gly Asp Lys Glu Ala Phe 130 135 140 Ser Ala Cys Met Pro Leu Leu Ser His Leu Gly Lys Asn Ile Val Trp 145 150 155 160 Gln Gly Glu Ala Gly Ala Gly Gln His Thr Lys Met Cys Asn Gln Ile 165 170 175 Ala Ile Ala Thr Asn Met Ile Gly Val Cys Glu Ala Leu Val Tyr Ala 180 185 190 Glu Lys Ala Gly Leu Asp Pro Glu Arg Val Leu Arg Ser Ile Ser Gln 195 200 205 Gly Ala Ala Ser Ser Trp Ser Leu Ser Asn Leu Ala Pro Arg Met Leu 210 215 220 Ala Gly Asp Phe Ala Pro Gly Phe Tyr Val Lys His Phe Ile Lys Asp 225 230 235 240 Met Gly Ile Ala Leu Ala Glu Ala Glu Thr Leu Gly Leu Ser Leu Pro 245 250 255 Gly Leu Lys Leu Ala Lys Gln Leu Tyr Glu Ser Leu Ala Glu Arg Gly 260 265 270 Glu Glu Gln Ser Gly Thr Gln Ala Leu Tyr Lys Trp Tyr Lys Glu Trp 275 280 285 <210> 122 <211> 296 <212> PRT <213> HIBADHbc <400> 122 Met Glu Ile Ala Phe Ile Gly Leu Gly Asn Met Gly Gly Pro Met Ala 1 5 10 15 Ala Asn Leu Leu Lys Ala Gly His Ala Leu Thr Val Phe Asp Leu Asp 20 25 30 Ala His Ala Val Asp Ala Ala Val Arg Ala Gly Ala Ala Ala Ala Thr 35 40 45 Ser Pro Arg Asp Ala Ala Ala Arg Gly Ser Leu Ile Ile Thr Met Leu 50 55 60 Pro Ala Ala Gln His Val Arg Ala Val Tyr Leu Gly Asp Asp Gly Val 65 70 75 80 Leu Ala Gly Ala Arg Ala Gly Ala Thr Leu Ile Asp Cys Ser Thr Ile 85 90 95 Asp Pro Gly Thr Val Arg Ala Val Ala Asp Ala Ala Ala Gln Arg Asp 100 105 110 Phe Pro Leu Ala Asp Ala Pro Val Ser Gly Gly Thr Gly Gly Ala Gln 115 120 125 Ala Gly Thr Leu Thr Phe Met Val Gly Ala Asp Gly Ala Leu Phe Glu 130 135 140 Arg Ile Arg Pro Val Leu Leu Asp Met Gly Arg Asn Val Val His Cys 145 150 155 160 Gly Gly Thr Gly Thr Gly Gln Ile Ala Lys Ile Cys Asn Asn Leu Leu 165 170 175 Leu Gly Ile Ser Met Met Gly Val Ser Glu Ala Met Ala Leu Gly Ala 180 185 190 Ala Leu Gly Ile Asp Pro Thr Val Leu Ala Gly Ile Ile Asn Thr Ser 195 200 205 Thr Gly Arg Cys Trp Ser Ser Asp Ala Tyr Asn Pro Tyr Pro Gly Val 210 215 220 Ser Asp Thr Ala Pro Ala Ala Arg Gly Tyr Ala Gly Gly Phe Ala Ala 225 230 235 240 Asn Leu Met Leu Lys Asp Leu Gly Leu Ala Thr Glu Ala Ala Arg Ser 245 250 255 Ala His Gln Pro Val Trp Met Gly Ala Leu Ala Gln Gln Leu Tyr Gln 260 265 270 Ser Met Ser Gln Gln Gly Leu Gly Thr Leu Asp Phe Ser Ala Cys Val 275 280 285 Lys Leu Tyr Glu Ala Gln Pro Ala 290 295 <210> 123 <211> 295 <212> PRT <213> HIBADHma <400> 123 Met Ala Lys Ile Thr Phe Ile Gly Leu Gly Asn Met Gly Gly Pro Met 1 5 10 15 Ala Gly Asn Leu Val Lys Ala Gly His Glu Val Thr Val Phe Asp Leu 20 25 30 Ser Lys Asp Ala Val Ala Ala Leu Val Ser Glu Gly Ala Lys Thr Ala 35 40 45 Gly Thr Ala His Glu Ala Ala Ser Gly Ala Glu Cys Val Ile Thr Met 50 55 60 Leu Pro Ala Gly Gln His Val Glu Ala Val Tyr Leu Gly Asp Asp Gly 65 70 75 80 Leu Leu Ala Lys Leu Pro Ala Gly Thr Leu Val Ile Asp Ser Ser Thr 85 90 95 Ile Ala Pro Glu Thr Ala Arg Gly Val Ala Glu Val Ala Arg Ala Lys 100 105 110 Asp Ile Pro Phe Leu Asp Ala Pro Val Ser Gly Gly Val Gly Gly Ala 115 120 125 Lys Ala Gly Thr Leu Thr Phe Ile Cys Gly Gly Ser Glu Glu Ala Phe 130 135 140 Asn Lys Ala Lys Pro Ile Leu Asp Thr Met Gly Lys Asn Ile Phe His 145 150 155 160 Ala Gly Asp His Gly Ser Gly Gln Val Ala Lys Ile Cys Asn Asn Met 165 170 175 Leu Leu Ala Ile Leu Met Ser Gly Thr Ser Glu Ala Leu Ala Leu Gly 180 185 190 Val Lys Asn Gly Leu Asp Pro Ala Val Leu Ser Glu Ile Met Lys Gln 195 200 205 Ser Ser Gly Gly Asn Trp Ala Leu Asn Val Tyr Asn Pro Trp Pro Gly 210 215 220 Val Met Glu Gly Val Pro Ala Ser Arg Asn Tyr Glu Gly Gly Phe Leu 225 230 235 240 Val Asn Leu Met Thr Lys Asp Leu Gly Leu Ala Phe Asp Asn Ala Val 245 250 255 Lys Asn Gln Ala Ser Ile Pro Met Gly Ser Leu Ala Arg Asn Leu Phe 260 265 270 Gln Leu His Ala Gly Gln Gly Asn Gly Ser Leu Asp Phe Ser Ser Ile 275 280 285 Gln Arg Leu Tyr Lys Pro Glu 290 295 <210> 124 <211> 298 <212> PRT <213> HIBADHpa <400> 124 Met Thr Asp Ile Ala Phe Leu Gly Leu Gly Asn Met Gly Gly Pro Met 1 5 10 15 Ala Ala Asn Leu Leu Lys Ala Gly His Arg Val Asn Val Phe Asp Leu 20 25 30 Gln Pro Lys Ala Val Leu Gly Leu Val Glu Gln Gly Ala Gln Gly Ala 35 40 45 Asp Ser Ala Leu Gln Cys Cys Glu Gly Ala Glu Val Val Ile Ser Met 50 55 60 Leu Pro Ala Gly Gln His Val Glu Ser Leu Tyr Leu Gly Asp Asp Gly 65 70 75 80 Leu Leu Ala Arg Val Ala Gly Lys Pro Leu Leu Ile Asp Cys Ser Thr 85 90 95 Ile Ala Pro Glu Thr Ala Arg Lys Val Ala Glu Ala Ala Ala Ala Lys 100 105 110 Gly Leu Thr Leu Leu Asp Ala Pro Val Ser Gly Gly Val Gly Gly Ala 115 120 125 Arg Ala Gly Thr Leu Ser Phe Ile Val Gly Gly Pro Ala Glu Gly Phe 130 135 140 Ala Arg Ala Arg Pro Val Leu Glu Asn Met Gly Arg Asn Ile Phe His 145 150 155 160 Ala Gly Asp His Gly Ala Gly Gln Val Ala Lys Ile Cys Asn Asn Met 165 170 175 Leu Leu Gly Ile Leu Met Ala Gly Thr Ala Glu Ala Leu Ala Leu Gly 180 185 190 Val Lys Asn Gly Leu Asp Pro Ala Val Leu Ser Glu Val Met Lys Gln 195 200 205 Ser Ser Gly Gly Asn Trp Ala Leu Asn Leu Tyr Asn Pro Trp Pro Gly 210 215 220 Val Met Pro Gln Ala Pro Ala Ser Asn Gly Tyr Ala Gly Gly Phe Gln 225 230 235 240 Val Arg Leu Met Asn Lys Asp Leu Gly Leu Ala Leu Ala Asn Ala Gln 245 250 255 Ala Val Gln Ala Ser Thr Pro Leu Gly Ala Leu Ala Arg Asn Leu Phe 260 265 270 Ser Leu His Ala Gln Ala Asp Ala Glu His Glu Gly Leu Asp Phe Ser 275 280 285 Ser Ile Gln Lys Leu Tyr Arg Gly Lys Asp 290 295 <210> 125 <211> 295 <212> PRT <213> HIBADHxc <400> 125 Met Ser Lys Ile Ala Phe Ile Gly Leu Gly Asn Met Gly Gly Pro Met 1 5 10 15 Val Ala Asn Leu Ile Lys Ala Gly His Glu Leu Arg Val Phe Asp Leu 20 25 30 Val His Ser Ala Leu Asp Thr Ala His Ala Ala Gly Ala Gln Val Ala 35 40 45 Ser Ser Ala Ser Asp Thr Leu His Gly Ala Glu Val Val Ile Ser Met 50 55 60 Leu Pro Ala Ser Lys His Val Glu Gly Leu Tyr Leu Gly Asp Ala Gly 65 70 75 80 Ile Leu Ala His Ile Pro Ala Gly Ala Leu Val Ile Asp Cys Ser Thr 85 90 95 Ile Ala Pro Ala Met Ala Arg Thr Val Ala Thr Ala Ala His Ala Arg 100 105 110 Gly Leu Asp Met Leu Asp Ala Pro Val Ser Gly Gly Thr Ala Gly Ala 115 120 125 Ala Ala Gly Thr Leu Thr Phe Ile Val Gly Gly Ala Ala Gln Ala Leu 130 135 140 Glu Arg Ala Arg Pro Val Leu Gln Ala Met Gly Lys Asn Leu Phe His 145 150 155 160 Val Gly Asp Asn Gly Ala Gly Gln Val Ala Lys Leu Cys Asn Asn Met 165 170 175 Ala Leu Gly Val Ile Met Ala Ala Thr Gly Glu Ala Ile Ala Leu Gly 180 185 190 Val Ala Gln Gly Leu Asp Pro Ala Val Leu Ser Gln Met Met Ala Val 195 200 205 Ser Thr Gly Arg Ser Trp Ala Thr Glu Val Cys Asn Pro Trp Pro Gly 210 215 220 Val Leu Glu Asn Ala Pro Ala Ser Arg Gly Tyr Ser Gly Gly Phe Gly 225 230 235 240 Asn Asp Leu Met Leu Lys Asp Leu Gly Leu Val Ala Glu Ala Ala Val 245 250 255 Gln Ala Gly Ala Ser Ile Pro Leu Gly Glu Leu Ala Arg Asn Leu Tyr 260 265 270 Ala Met Asn Ser Arg Ala Gly Asn Gly Ala Leu Asp Phe Ser Ser Val 275 280 285 Val Lys Leu Val Ala Lys Val 290 295 <210> 126 <211> 253 <212> PRT <213> HPDHam <400> 126 Met Thr Gln Gln Thr Val Phe Val Thr Gly Ala Ser Ala Gly Phe Gly 1 5 10 15 Ala Ala Ile Ala Arg Arg Phe Val Ala Glu Gly Ala Arg Val Ile Ala 20 25 30 Val Ala Arg Ser Glu Asp Lys Leu Glu Lys Leu Ala Gly Glu Leu Gly 35 40 45 Asp Ala Val Leu Pro Val Lys Leu Asp Val Ser Asp Pro Glu Ala Val 50 55 60 Lys Glu Val Val Thr Asn Leu Pro Ala Asp Trp Gln Gln Val Asp Val 65 70 75 80 Leu Val Asn Asn Ala Gly Leu Ala Lys Gly Leu Glu Pro Ala His Lys 85 90 95 Ala Asp Leu Gln Asp Trp Asp Glu Met Ile Ala Thr Asn Val Arg Gly 100 105 110 Leu Thr His Val Thr Arg Ala Leu Leu Pro Gly Met Val Glu Arg Gly 115 120 125 Arg Gly His Val Ile Asn Ile Gly Ser Ile Ala Gly Thr Tyr Pro Tyr 130 135 140 Pro Gly Gly Asn Val Tyr Gly Ala Thr Lys Ala Phe Val His Gln Phe 145 150 155 160 Ser Leu Asn Leu Arg Ser Asp Leu His Gly Thr Gly Val Arg Val Thr 165 170 175 Asn Val Glu Pro Gly Met Val Gly Gly Thr Asp Phe Ser Lys Val Arg 180 185 190 Phe Asp Gly Asp Gln Ala Lys Ala Asp Lys Val Tyr Glu Gly Thr Thr 195 200 205 Pro Leu Thr Ala Glu Asp Val Ala Glu Ser Val Phe Trp Ala Ala Asn 210 215 220 Gln Pro Ala His Val Asn Ile Asn Val Ile Glu Leu Met Pro Val Val 225 230 235 240 Gln Ser Phe Ser Pro Leu Gln Ile Phe Arg Asp Asn Ser 245 250 <210> 127 <211> 550 <212> PRT <213> HPDHbs <400> 127 Met Thr Ala Gly Lys Gly Lys Gly Ala Lys Val Asp Pro Ala Gly Gly 1 5 10 15 Lys Ala Ala Thr Gly Arg Leu Ala Gly Lys Val Ala Leu Val Thr Gly 20 25 30 Ala Ala Gly Asn Leu Gly Gly Ala Ile Ile Arg Arg Phe Ile Ala Glu 35 40 45 Gly Ala Thr Leu Val Met Thr Gly Arg Thr Lys Glu Arg Leu Ala Ala 50 55 60 Ala Lys Gln Ala Leu Leu Asp Glu Thr Gly Val Glu Glu Ala Arg Val 65 70 75 80 Phe Thr Val Ile Leu Asp Gly Ala Asp Pro Asp Ser Val Arg Ala Gly 85 90 95 Ile Asp Gln Val Arg Ala Ala Leu Gly Arg Ile Asp Val Leu Val Asn 100 105 110 Asn Ala Gly Ser Ala Gly Pro Arg Gln Thr Leu Glu Asn Val Pro Leu 115 120 125 Thr Arg Ala Glu Met Glu Ala Asn Gly Asp Ser Glu Thr Val Ala Asp 130 135 140 Ala Met Arg Asn Leu Leu Gly Val Ser Trp Asn Leu Thr Arg Ala Ala 145 150 155 160 Ala Pro Ile Met Pro Val Gly Ala Ser Val Val Asn Ile Ser Thr Ile 165 170 175 Phe Ser His Thr Arg Tyr Tyr Gly Arg Thr Ala Tyr Val Val Pro Lys 180 185 190 Ala Ala Leu Asn Ala Leu Ser Gln Glu Leu Ala Lys Glu Leu Gly Ala 195 200 205 Ser Gly Ile Arg Val Asn Thr Val Phe Pro Gly Pro Ile Glu Ser Glu 210 215 220 Arg Ile Arg Thr Val Phe Ala Ala Met Asp Ala Leu Gln Gly Glu Pro 225 230 235 240 Glu Asn Ser Thr Ala Asn His Phe Met Gly Arg Met Thr Leu Glu Arg 245 250 255 Ala Ile Gly Asp Ala Pro Lys Arg Lys Thr Leu Pro Val Pro Gly Asp 260 265 270 Ile Ala Asp Ala Cys Leu Phe Leu Ala Ser Asp Glu Ser Ala Ala Leu 275 280 285 Asn Gly Asn Glu Ile Asp Val Thr His Gly Met Gln Val Arg Lys Asp 290 295 300 Ser Arg Ser Thr Tyr Met Thr Arg Pro Ser Met Arg Ser Leu Asp Gly 305 310 315 320 Ala Gly Leu Thr Val Leu Ile Ala Ala Gly Glu Ala Trp Glu Asp Ala 325 330 335 Leu Asp Met Ala Lys Ile Gln Ala Ala Cys Gly Ala Arg Val Leu Leu 340 345 350 Gly Met Thr Arg Gln Val Asp Val Ala Gln Ala Glu Ala Arg Val Lys 355 360 365 Ala Gln Gly Ala Glu Glu Gly Leu Thr Ile Met Arg Phe Asn Arg Ala 370 375 380 Glu Pro Asp Gly Met Glu Ala Ala Ile Arg Ala Tyr Ser Asp Ala His 385 390 395 400 Gly Ala Ile His Ser Ala Ile Val Leu Pro Val Lys Pro Ala Gly His 405 410 415 Phe Gly Asp Ser Leu Val Glu Ala Ser Asp Ala Glu Val Asp Ala Leu 420 425 430 Ile Asp Ile Glu Leu Thr Gly Ala Ile Ala Val Ala Arg Thr Leu Arg 435 440 445 Arg Tyr Trp Lys Thr Cys Ser Asp Leu Met Gln Asp Pro Arg Phe Val 450 455 460 Phe Met Ser Gly Pro Ser Asp Gly Lys Gly Asn Lys Ala Gly Arg Ile 465 470 475 480 Leu Ala Ala Ala Ile Ala Gln Leu Ile Thr Ile Trp Arg Asp Glu Ser 485 490 495 Arg Val Asp Phe Ala Gln Gly Arg Gln Lys Arg Ala Ala Trp Gly Asn 500 505 510 Gln Ile Val Arg Tyr Thr Asn Glu Glu Asp Glu Asn Thr Arg Phe Ala 515 520 525 Ala Gly His Ala Thr Arg Ile Val Phe Lys Glu Gln Lys Ile Thr Glu 530 535 540 Thr Ser Leu Tyr Leu Pro 545 550 <210> 128 <211> 550 <212> PRT <213> HPDHca <400> 128 Met Ser Gly Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly 1 5 10 15 Gly Ala Gly Asn Ile Gly Ser Glu Leu Thr Arg Arg Phe Leu Ala Glu 20 25 30 Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Arg Ala Lys Leu Thr Ala 35 40 45 Leu Ala Glu Arg Met Gln Ala Glu Ala Gly Val Pro Ala Lys Arg Ile 50 55 60 Asp Leu Glu Val Met Asp Gly Ser Asp Pro Val Ala Val Arg Ala Gly 65 70 75 80 Ile Glu Ala Ile Val Ala Arg His Gly Gln Ile Asp Ile Leu Val Asn 85 90 95 Asn Ala Gly Ser Ala Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu 100 105 110 Thr Glu Ala Glu Leu Gly Pro Gly Ala Glu Glu Thr Leu His Ala Ser 115 120 125 Ile Ala Asn Leu Leu Gly Met Gly Trp His Leu Met Arg Ile Ala Ala 130 135 140 Pro His Met Pro Val Gly Ser Ala Val Ile Asn Val Ser Thr Ile Phe 145 150 155 160 Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Thr Pro Lys Ala 165 170 175 Ala Leu Asn Ala Leu Ser Gln Leu Ala Ala Arg Glu Leu Gly Ala Arg 180 185 190 Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Asp Arg 195 200 205 Ile Arg Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu 210 215 220 Gly Asp Thr Ala His His Phe Leu Asn Thr Met Arg Leu Cys Arg Ala 225 230 235 240 Asn Asp Gln Gly Ala Leu Glu Arg Arg Phe Pro Ser Val Gly Asp Val 245 250 255 Ala Asp Ala Ala Val Phe Leu Ala Ser Ala Glu Ser Ala Ala Leu Ser 260 265 270 Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Ala Cys Ser 275 280 285 Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Ser 290 295 300 Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met 305 310 315 320 Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly 325 330 335 Phe Arg Ser Ala Ala Ala Leu Ala Gln Phe Glu Gln Ala Val Asn Glu 340 345 350 Ser Arg Arg Leu Ala Gly Ala Asp Phe Thr Pro Pro Ile Ala Leu Pro 355 360 365 Leu Asp Pro Arg Asp Pro Ala Thr Ile Asp Ala Val Phe Asp Trp Gly 370 375 380 Ala Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala 385 390 395 400 Thr Ser His Glu Pro Ala Pro Cys Val Ile Glu Val Asp Asp Glu Arg 405 410 415 Val Leu Asn Phe Leu Ala Asp Glu Ile Thr Gly Thr Ile Val Ile Ala 420 425 430 Ser Arg Leu Ala Arg Tyr Trp Gln Ser Gln Arg Leu Thr Pro Gly Ala 435 440 445 Arg Ala Arg Gly Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Asp Gln 450 455 460 Asn Gly Asn Val Tyr Gly Arg Ile Gln Ser Ala Ala Ile Gly Gln Leu 465 470 475 480 Ile Arg Val Trp Arg His Glu Ala Glu Leu Asp Tyr Gln Arg Ala Ser 485 490 495 Ala Ala Gly Asp His Val Leu Pro Pro Val Trp Ala Asn Gln Ile Val 500 505 510 Arg Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp 515 520 525 Thr Ala Gln Leu Leu His Ser Gln Arg His Ile Asn Glu Ile Thr Leu 530 535 540 Asn Ile Pro Ala Asn Ile 545 550 <210> 129 <211> 550 <212> PRT <213> HPDHcag <400> 129 Met Ser Val Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly 1 5 10 15 Gly Ala Gly Asn Ile Gly Ser Glu Met Thr Arg Arg Phe Leu Ala Glu 20 25 30 Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Ser Ala Lys Leu Ala Ala 35 40 45 Leu Ala Glu Arg Leu Arg Ser Glu Ala Gly Val Pro Ala Lys Arg Ile 50 55 60 Asp Leu Glu Val Met Asp Gly Ser Asp Pro Ala Ala Val Arg Ala Gly 65 70 75 80 Val Ala Ala Ile Ile Gly Arg His Gly His Ile Asp Ile Leu Val Asn 85 90 95 Asn Ala Gly Ser Thr Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu 100 105 110 Asn Glu Thr Asp Arg Asp Leu Asp Asp Glu Glu Ala Leu Ser Thr Ser 115 120 125 Val Ala Asn Leu Leu Gly Met Ala Trp His Leu Met Arg Ile Leu Ser 130 135 140 Pro His Met Pro Pro Gly Ser Ala Ile Ile Asn Ile Ser Thr Ile Phe 145 150 155 160 Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ala 165 170 175 Ala Leu Asn Thr Leu Thr Gln Ile Ala Ala Arg Glu Leu Gly Ile Arg 180 185 190 Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Glu Arg 195 200 205 Ile Gln Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu 210 215 220 Gly Asp Thr Ala Ser Gln Phe Leu Ala Thr Met Arg Leu Tyr Arg Ala 225 230 235 240 Asn Asp Gln Gly Gln Leu Glu Arg Arg Phe Pro Thr Ile Cys Asp Val 245 250 255 Ala Asp Ala Ala Val Phe Leu Ala Ser Asp Glu Ala Ala Ala Leu Thr 260 265 270 Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Thr Ser Ser 275 280 285 Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Asn 290 295 300 Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met 305 310 315 320 Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly 325 330 335 Phe Arg Ser Glu Ala Ala Leu Ala Gln Phe Glu Gln Ala Ile Gly Glu 340 345 350 Ser Arg Arg Leu Ala Gly Glu Ser Phe Ile Pro Pro Ile Ala Leu Pro 355 360 365 Ile Asp Leu Arg Asn Pro Ser Thr Ile Asp Ala Leu Phe Asp Trp Ala 370 375 380 Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala Ser 385 390 395 400 Gly Arg Glu Pro Ala Thr Gln Val Ile Asp Ile Asp Asp Ala His Val 405 410 415 Gln Ala Phe Leu Asn Asp Glu Ile Val Gly Ser Ile Ile Ile Ala Ser 420 425 430 Arg Leu Ala Arg Tyr Trp Gln Ala Gln Arg Ile Ala Pro Gly Ala Arg 435 440 445 Ala Arg Glu Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Ser Thr Ala 450 455 460 Gly Asn Pro Tyr Gly Arg Ile Gln Ser Ala Ala Ile Glu Gln Leu Ile 465 470 475 480 Arg Val Trp Arg His Glu Ala Ala Leu Asp Tyr Glu Arg Ala Thr Ala 485 490 495 Ala Gly Glu Arg Val Leu Pro Ala Val Trp Ala Ser Gln Ile Val Arg 500 505 510 Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp Thr 515 520 525 Ala Gln Leu Leu His Ser Gln Arg Arg Ile Asn Glu Ile Thr Leu Thr 530 535 540 Ile Pro Ala Asp Ile Ser 545 550 <210> 130 <211> 550 <212> PRT <213> HPDHct <400> 130 Met Arg Arg Thr Lys Pro Glu Cys Leu Arg Arg Asn Glu Ser Thr Met 1 5 10 15 Arg Leu Asn Gly Lys Val Ala Leu Ile Thr Gly Ala Ala Gly Gly Ile 20 25 30 Gly Ser Val Val Thr Arg Met Tyr Leu Arg Glu Gly Ala Arg Val Ala 35 40 45 Ile Ser Ser Arg Ser Leu Glu Arg Ala Glu Gln Phe Arg Ala Ala Leu 50 55 60 Met Ala Glu Gly Phe Ser Gly Asp Asp Ile Leu Pro Val Ala Phe Ala 65 70 75 80 Ser Thr Asp Leu Ala Gly Met Gln Thr Ala Ile Glu Ala Ile Ala Ala 85 90 95 Arg Trp Gly Gly Leu Asp Val Leu Ile Asn Asn Ala Gly Ser Ala Gly 100 105 110 Ala Lys Gln Pro Leu Phe Arg Ile Pro Phe Thr Ser Glu Asp Leu Ala 115 120 125 Gln Leu Ala Ala Glu Gly Phe Thr Glu Thr Glu Thr Met Arg Glu Ser 130 135 140 Ala Ala Asn Leu Leu Gly Met Pro Trp His Leu Thr Arg Leu Ala Leu 145 150 155 160 Pro Tyr Leu Arg Val Gly Ala Ser Ile Ile Asn Val Ser Thr Ile Phe 165 170 175 Ser Arg Thr Asn Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ser 180 185 190 Ala Leu Asn Ala Leu Ser Leu Gly Leu Ala Lys Gln Leu Gly Thr Thr 195 200 205 Asp Arg Ala Val Arg Val Asn Thr Val Phe Pro Gly Pro Ile Asp Ser 210 215 220 Asp Arg Ile Arg Thr Val Phe Gly Ala Met Asp Arg Leu Lys Gly Val 225 230 235 240 Pro Glu Gly Thr Thr Ala Gln Glu Phe Phe Asp Ile Met Ile Leu Ala 245 250 255 Arg Ala Arg Gly Gly Glu Ala Pro Thr Lys Cys Tyr Pro Thr Gln Ala 260 265 270 Asp Val Ala His Val Ile Leu Phe Leu Gly Ser Asp Glu Ser Ala Ala 275 280 285 Ile Ser Gly His Asn Phe Glu Ile Thr His Gly Met Gln Val Lys Ala 290 295 300 Gln Ser Arg Thr Lys Leu Thr Ala Trp Pro Asp Gln Arg Leu Ile Asp 305 310 315 320 Leu Ser Arg Arg Val Val Leu Ile Val Gly Gly Glu Gln Val His Asp 325 330 335 Ala Val Thr Ile Ala Ala Ser Gln Gln Ser Tyr Gly Ala Ser Val Ile 340 345 350 Leu Thr Phe Arg Gln Pro Glu Ala Val Asn Thr Ala Lys Ala Gln Leu 355 360 365 Glu Ser Arg Gly Leu Asn His Ser Ile Ala Val Thr Trp Leu Asp Pro 370 375 380 Leu Arg Arg Glu Ser Val Thr Ser Val Phe Asp Leu Ile Arg Glu Arg 385 390 395 400 Tyr His Arg Leu Asp Thr Val Ile Val Phe Pro Ala His Ala Ala Gly 405 410 415 His Tyr Gly Pro Glu Leu Ile Pro Asp Glu Pro Glu Arg Ile Arg Gln 420 425 430 Phe Thr Asp Glu Ala Ile Leu Gly Thr Thr Ala Phe Ala Ala Gln Leu 435 440 445 Ser Arg Phe Leu Leu Ala Phe Asp Ala Glu Asn Glu Leu Gln Glu Pro 450 455 460 Val Asn Val Leu Phe Val Thr Asn Glu His Asp Gly Ala Thr Asn Ala 465 470 475 480 Phe Glu Asp Ile Tyr Arg Ala Ala Val Glu Gln Leu Leu Arg Val Trp 485 490 495 Arg His Glu Asp Glu Leu Gln Val Ala Ala Gly Thr Arg Arg Phe Ala 500 505 510 Val Arg Thr Asn Gln Ile Val Arg Tyr Ala Asn Ala Glu Pro Asp Asn 515 520 525 Leu Lys Phe Thr Ala Asp Trp Leu Ala Thr Leu Ala Asn Arg Val Arg 530 535 540 Ile Phe Asp Glu Leu Asn 545 550 <210> 131 <211> 248 <212> PRT <213> HPDHec <400> 131 Met Ile Val Leu Val Thr Gly Ala Thr Ala Gly Phe Gly Glu Cys Ile 1 5 10 15 Thr Arg Arg Phe Ile Gln Gln Gly His Lys Val Ile Ala Thr Gly Arg 20 25 30 Arg Gln Glu Arg Leu Gln Glu Leu Lys Asp Glu Leu Gly Asp Asn Leu 35 40 45 Tyr Ile Ala Gln Leu Asp Val Arg Asn Arg Ala Ala Ile Glu Glu Met 50 55 60 Leu Ala Ser Leu Pro Ala Glu Trp Cys Asn Ile Asp Ile Leu Val Asn 65 70 75 80 Asn Ala Gly Leu Ala Leu Gly Met Glu Pro Ala His Lys Ala Ser Val 85 90 95 Glu Asp Trp Glu Thr Met Ile Asp Thr Asn Asn Lys Gly Leu Val Tyr 100 105 110 Met Thr Arg Ala Val Leu Pro Gly Met Val Glu Arg Asn His Gly His 115 120 125 Ile Ile Asn Ile Gly Ser Thr Ala Gly Ser Trp Pro Tyr Ala Gly Gly 130 135 140 Asn Val Tyr Gly Ala Thr Lys Ala Phe Val Arg Gln Phe Ser Leu Asn 145 150 155 160 Leu Arg Thr Asp Leu His Gly Thr Ala Val Arg Val Thr Asp Ile Glu 165 170 175 Pro Gly Leu Val Gly Gly Thr Glu Phe Ser Asn Val Arg Phe Lys Gly 180 185 190 Asp Asp Gly Lys Ala Glu Lys Thr Tyr Gln Asn Thr Val Ala Leu Thr 195 200 205 Pro Glu Asp Val Ser Glu Ala Val Trp Trp Val Ser Thr Leu Pro Ala 210 215 220 His Val Asn Ile Asn Thr Leu Glu Met Met Pro Val Thr Gln Ser Tyr 225 230 235 240 Ala Gly Leu Asn Val His Arg Gln 245 <210> 132 <211> 196 <212> PRT <213> HPDHed <400> 132 Met Ser Glu Ala Ile Thr Pro Gly Ala Leu Glu Thr Leu Phe Thr Gly 1 5 10 15 Ala Arg Thr His Asn Gly Trp Gln Asn Met Pro Val Ser Asp Glu Thr 20 25 30 Leu Arg Glu Ile Tyr Asn Leu Met Lys Trp Gly Pro Thr Ser Ala Asn 35 40 45 Cys Ser Pro Ala Arg Ile Val Phe Val Arg Ser Ala Glu Gly Lys Glu 50 55 60 Lys Leu Arg Pro Ala Leu Ser Ser Gly Asn Leu Glu Lys Thr Leu Thr 65 70 75 80 Ala Pro Val Thr Ala Ile Val Ala Trp Asp Gly Glu Phe Tyr Glu Arg 85 90 95 Leu Pro Glu Leu Phe Pro His Gly Asp Ala Lys Ser Trp Phe Thr Ala 100 105 110 Ser Pro Ala Leu Ala Glu Glu Thr Ala Phe Arg Asn Ser Ser Leu Gln 115 120 125 Ala Ala Tyr Leu Ile Phe Ala Cys Arg Ala Leu Gly Leu Asp Thr Gly 130 135 140 Pro Met Ser Gly Phe Asp Arg Gln Lys Val Asp Glu Ala Phe Phe Thr 145 150 155 160 Gly Thr Thr Leu Lys Ser Asn Leu Leu Ile Asn Ile Gly Tyr Gly Asp 165 170 175 Pro Gly Lys Leu Tyr Gly Arg Leu Pro Arg Leu Asp Phe Asp Asp Ala 180 185 190 Cys Gly Leu Ala 195 <210> 133 <211> 550 <212> PRT <213> HPDHgb <400> 133 Met Asn Asp Met Ser Asp Ile Pro Gln Asp Leu Leu Thr Gln Ser Ile 1 5 10 15 Ala Gly Arg Arg Leu Asp Asn Lys Cys Ile Ile Leu Thr Gly Ala Ala 20 25 30 Gly Ser Ile Gly Ser Phe Ile Thr Arg Gln Leu Leu Arg Glu Gly Ala 35 40 45 Arg Val Met Leu Thr Gly Arg Asp Gln Ser Lys Leu Asp Asp Phe Ile 50 55 60 Gly Asp Leu Val Ser Glu Gly Phe Glu Glu Thr Phe Met Val Ser Ala 65 70 75 80 Thr Gly Asp Cys Ala Asp Pro Glu Val Cys Arg Glu Ile Val Ala Lys 85 90 95 Thr Val Ala Ala Phe Gly Pro Ile Asp Val Leu Val Asn Asn Ala Gly 100 105 110 Ala Ala Gly Pro Lys Phe Thr Leu Arg Asp Ile Pro Phe Thr Glu Ala 115 120 125 Glu Gln Arg Ala Ala Asp Ser Asp Gln Thr Met Phe Asp Ser Ala Met 130 135 140 Asn Leu Leu Gly Ala Pro Trp Asn Met Ala Arg Ala Ala Ala Pro His 145 150 155 160 Met Ser Ala Gly Ala Ser Ile Ile Asn Val Ser Thr Ile Phe Ser Arg 165 170 175 Thr His Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ser Gly Leu 180 185 190 Asn Ala Leu Gly Lys Gly Leu Ala Leu Glu Leu Gly Glu Lys Gln Gly 195 200 205 Ile Arg Val Asn Thr Leu Phe Pro Gly Pro Ile Glu Ser Glu Arg Ile 210 215 220 Glu Ser Val Phe Ala Arg Met Asp Glu Leu Gln Gly Val Glu Pro Gly 225 230 235 240 Ser Thr Gly Lys Glu Phe Arg Asp Leu Met Ile Thr Thr Arg Thr Ser 245 250 255 Asp Gly Gly Leu Glu Tyr Lys Tyr Pro Thr Pro Thr Asp Val Ala Ser 260 265 270 Gly Ile Val Trp Leu Ala Ser Asp Glu Ser Ser Ala Leu Ser Gly His 275 280 285 His Ile Glu Val Thr Asn Gly Met Gln Val Pro Ala Gln Ser Arg Ser 290 295 300 Gln Leu Val Ser Trp Pro Asp Lys Arg Leu Glu Asp Leu Ser Gly Gln 305 310 315 320 Val Val Leu Ile Leu Gly Gly Asp Asp Tyr Gln Glu Ala Val Thr Phe 325 330 335 Ala Glu Arg Gln Ile Gln Ser Gly Ala Arg Val Met Leu Ala Phe Arg 340 345 350 Asn Leu Asn Thr Val Gly His Ala Arg Ser Leu Val Gln Ser Arg Asn 355 360 365 Leu Gly Asp Val Gln Leu Ser His Leu Asp Pro Leu Arg Arg Glu Ser 370 375 380 Val Asp Arg Ser Met Gln Leu Ile Ala Asp His Phe Gly Arg Leu Asp 385 390 395 400 Gly Val Ile Leu Leu Pro Gln Lys Pro Asn Gly Glu Tyr Gly Tyr Ser 405 410 415 Leu Thr Thr Ala Asn Asp Glu Asp Val Ala Asn Phe Val Arg Asp Glu 420 425 430 Val Val Ala Pro Val Ala Phe Ala Ser Ala Phe Ala Arg Asn Met Asp 435 440 445 Gln Leu Phe Ala Glu Gly Glu Pro Pro Ala Ile Val Tyr Val Thr Asn 450 455 460 Pro Ser Asp Arg His Gly Asn Leu Met Asn Glu Ile Ile Arg Ala Ser 465 470 475 480 Val Glu Ala Leu Ile Arg Gly Trp Arg His Glu Asp Glu Thr Leu Ala 485 490 495 Asn Thr Gly Glu Leu Pro Trp Ala Val Gln Pro Asn Gln Met Val Arg 500 505 510 Tyr Asp Thr Glu Asp Arg Glu Ala Leu Thr Phe Ala Ala Asp Trp Ala 515 520 525 Ala Thr Leu Thr Asn Arg Val Arg Lys Met Asp Ser Ile Asn Leu Trp 530 535 540 Leu Pro Arg Ser Ile Lys 545 550 <210> 134 <211> 252 <212> PRT <213> HPDHhw <400> 134 Met Ile Val Leu Val Thr Gly Ala Ser Ser Gly Phe Gly Arg Ala Ile 1 5 10 15 Ala Lys Lys Phe Gly Glu Asn Gly His Lys Val Ile Ala Leu Ala Arg 20 25 30 Arg Lys Glu Arg Leu Gln Ser Leu Gln Lys Glu Ile Pro Tyr Cys Glu 35 40 45 Ile Leu Pro Cys Asp Ile Cys Asp Lys Lys Ala Val Lys Gln Ala Leu 50 55 60 Glu Ala Leu Pro Gln Asp Phe Lys Ser Ile Asp Ile Leu Val Asn Asn 65 70 75 80 Ala Gly Leu Ala Leu Gly Ile Glu Pro Ala Gln Met Cys Asp Phe Lys 85 90 95 Asp Trp Glu Arg Met Ile Ser Val Asn Val Thr Ala Leu Ser Tyr Leu 100 105 110 Thr His Leu Val Leu Pro Gln Met Val Glu Arg Lys Ser Gly His Ile 115 120 125 Ile Thr Leu Gly Ser Ile Ala Gly Lys Tyr Pro Tyr Pro Gly Gly Asn 130 135 140 Val Tyr Gly Ala Thr Lys Ala Phe Val Lys Gln Phe Ala Leu Gly Leu 145 150 155 160 Arg Ala Asp Leu Ala Gly Thr Asn Val Arg Val Ser Asp Ile Gln Pro 165 170 175 Gly Leu Ala Glu Ser Glu Phe Ser Leu Val Arg Phe Lys Gly Asp Lys 180 185 190 Lys Arg Ala Asn Ala Leu Tyr Glu Gly Ser Asn Ala Leu Gln Pro Gln 195 200 205 Asp Ile Ala Glu Ala Val Tyr Trp Val Ala Thr Leu Pro Lys His Ile 210 215 220 Asn Ile Asn Thr Leu Glu Met Met Pro Thr Ser Gln Ser Phe Ser Thr 225 230 235 240 Leu Ala Val His Lys Glu Tyr Ser Phe Leu Thr Lys 245 250 <210> 135 <211> 267 <212> PRT <213> HPDHka <400> 135 Met Ser Gln Gly Arg Arg Ala Ala Asp Arg Leu Ala Gly Lys Val Val 1 5 10 15 Leu Ile Thr Gly Ala Ser Ala Gly Ile Gly Glu Ala Thr Ala Trp Glu 20 25 30 Tyr Leu Phe Ala Ser Asn Gly Gln Ala Lys Leu Ile Leu Thr Ala Arg 35 40 45 Arg Leu Glu Lys Leu Ala Ala Ile Lys Glu Glu Met Lys Lys Glu Tyr 50 55 60 Pro Asn Val Lys Ile His Val Ala Lys Leu Asp Val Ser Asn Thr Glu 65 70 75 80 Glu Ile Lys Ser Leu Leu Gln Cys Leu Pro Thr Glu Phe Lys Ser Ile 85 90 95 Asp Ile Leu Val Asn Asn Ala Gly Lys Ala Leu Gly Ser Glu Lys Val 100 105 110 Gly Asp Ile Ala Ile Glu Asp Ile Lys Gly Met Met Asp Thr Asn Val 115 120 125 Ile Gly Leu Ile Asn Val Thr Gln Ala Val Leu Pro Ile Leu Lys Glu 130 135 140 Lys Asn Ser Gly Asp Ile Val Asn Ile Gly Ser Val Ala Gly Arg Asp 145 150 155 160 Ala Tyr Pro Thr Gly Ser Ile Tyr Cys Ala Ser Lys Phe Ala Val Arg 165 170 175 Ala Phe Thr Glu Ser Leu Arg Lys Glu Leu Leu Asp Thr Gly Ile Arg 180 185 190 Val Ile Leu Ile Ala Pro Gly Leu Val Glu Thr Glu Phe Ser Leu Val 195 200 205 Arg Tyr Lys Gly Asp Glu Lys Arg Ala Asn Ser Val Tyr Glu Gly Ile 210 215 220 Thr Ala Leu Glu Ala Glu Asp Val Ala Glu Leu Ile Val Phe Ser Thr 225 230 235 240 Ser Arg Lys Pro Asn Thr Val Ile Ala Asp Thr Leu Ile Phe Pro Thr 245 250 255 Cys Gln Gly Ser Ala Phe His Val His Arg Ser 260 265 <210> 136 <211> 314 <212> PRT <213> HPDHms <400> 136 Met Thr Glu Lys Val Ser Val Val Gly Ala Gly Val Ile Gly Val Gly 1 5 10 15 Trp Ala Thr Leu Phe Ala Ser Lys Gly Tyr Ser Val Ser Leu Tyr Thr 20 25 30 Glu Lys Lys Glu Thr Leu Asp Lys Gly Ile Glu Lys Leu Arg Asn Tyr 35 40 45 Val Gln Val Met Lys Asn Asn Ser Gln Ile Thr Glu Asp Val Asn Thr 50 55 60 Val Ile Ser Arg Val Ser Pro Thr Thr Asn Leu Asp Glu Ala Val Arg 65 70 75 80 Gly Ala Asn Phe Val Ile Glu Ala Val Ile Glu Asp Tyr Asp Ala Lys 85 90 95 Lys Lys Ile Phe Gly Tyr Leu Asp Ser Val Leu Asp Lys Glu Val Ile 100 105 110 Leu Ala Ser Ser Thr Ser Gly Leu Leu Ile Thr Glu Val Gln Lys Ala 115 120 125 Met Ser Lys His Pro Glu Arg Ala Val Ile Ala His Pro Trp Asn Pro 130 135 140 Pro His Leu Leu Pro Leu Val Glu Ile Val Pro Gly Glu Lys Thr Ser 145 150 155 160 Met Glu Val Val Glu Arg Thr Lys Ser Leu Met Glu Lys Leu Asp Arg 165 170 175 Ile Val Val Val Leu Lys Lys Glu Ile Pro Gly Phe Ile Gly Asn Arg 180 185 190 Leu Ala Phe Ala Leu Phe Arg Glu Ala Val Tyr Leu Val Asp Glu Gly 195 200 205 Val Ala Thr Val Glu Asp Ile Asp Lys Val Met Thr Ala Ala Ile Gly 210 215 220 Leu Arg Trp Ala Phe Met Gly Pro Phe Leu Thr Tyr His Leu Gly Gly 225 230 235 240 Gly Glu Gly Gly Leu Glu Tyr Phe Phe Asn Arg Gly Phe Gly Tyr Gly 245 250 255 Ala Asn Glu Trp Met His Thr Leu Ala Lys Tyr Asp Lys Phe Pro Tyr 260 265 270 Thr Gly Val Thr Lys Ala Ile Gln Gln Met Lys Glu Tyr Ser Phe Ile 275 280 285 Lys Gly Lys Thr Phe Gln Glu Ile Ser Lys Trp Arg Asp Glu Lys Leu 290 295 300 Leu Lys Val Tyr Lys Leu Val Trp Glu Lys 305 310 <210> 137 <211> 550 <212> PRT <213> HPDHot <400> 137 Met Phe Met Thr Arg Leu Asn Asp Lys Ile Ala Leu Ile Thr Gly Gly 1 5 10 15 Ala Gly Thr Ile Gly Glu Val Ile Thr Arg Arg Tyr Leu Glu Glu Gly 20 25 30 Ala Thr Val Val Met Ala Gly Arg Asn Arg Asp Lys Leu Asp Arg Tyr 35 40 45 Arg Glu Arg Leu Ile Thr Glu Phe His Ala Leu Pro Glu Arg Val Met 50 55 60 Val Val Arg Met Asp Gly Ser Ser Asn Ala Glu Val Arg Met Gly Ile 65 70 75 80 Ala Ala Val Val Ala His Phe Gly Arg Ile Asp Ile Leu Val Asn Asn 85 90 95 Ala Gly Ser Ala Gly Ala Arg Gln Arg Leu Pro Ala Ile Pro Leu Leu 100 105 110 Arg Ser Glu Leu Gln Ala Asp Glu Thr Glu Thr Leu Ala Asp Ser Ile 115 120 125 Gly Asn Leu Ile Gly Ile Thr Trp Asn Leu Ile Arg Ala Ala Ala Pro 130 135 140 Phe Met Pro Ala Gly Ser Ser Val Ile Asn Ile Ser Thr Ile Phe Ala 145 150 155 160 Arg Thr Asp Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ala Ala 165 170 175 Leu His Ala Leu Thr Leu Ala Ala Ala Thr Glu Leu Gly Glu Arg Gly 180 185 190 Ile Arg Val Asn Gln Ile Asn Pro Gly Pro Ile Asp Ser Asp Arg Ile 195 200 205 Arg Thr Val Phe Arg Arg Met Asp Glu Leu Lys Gly Val Pro Glu Gln 210 215 220 Ser Thr Ala Asp Gly Phe Phe Gln Met Met Arg Leu Arg Arg Pro Asn 225 230 235 240 Ala Glu Gly Asp Leu Val Lys Gly Phe Pro Lys Thr Leu Asp Val Ala 245 250 255 Asn Val Ala Val Phe Leu Gly Ser Ala Glu Ser Ala Ala Leu Ser Gly 260 265 270 Glu Thr Leu Asp Val Thr His Gly Met Ala Val Pro Thr Glu Ser Arg 275 280 285 Thr Thr Leu Thr Ser Arg Pro Gly Leu Arg Ala Val Asp Gly Ser Gly 290 295 300 His Thr Thr Leu Ile Cys Val Gly Asp Gln Ile Glu Glu Ala Ala Ala 305 310 315 320 Leu Thr Gly Val Leu Arg Ala Cys Gly Ala Glu Val Val Ile Gly Phe 325 330 335 Arg Ser Arg Ala Ala Ile Ala Arg Phe Asp His Leu Ile Glu Arg Gly 340 345 350 Arg His Leu Pro Ser Gln Glu His Val Ala Pro Val Leu Leu Tyr Leu 355 360 365 Asn Pro Thr Glu Pro Glu Ser Ile Asp Gln Ala Leu Arg Trp Met Ala 370 375 380 Thr Asn Leu Asp Leu Pro Thr Ser Val Ile Ile Leu Pro Ala Gln Arg 385 390 395 400 Gln Pro Leu Pro Pro Ser Val Val Arg Ala Ser Asp Glu Glu Val Ala 405 410 415 Tyr Phe Leu Arg Asp Glu Leu Ser Gly Met Ile Val Leu Ala Ser Arg 420 425 430 Leu Ala Arg Phe Trp Gln Gln Ala Thr Leu Ala Pro Gly Asn Ala Pro 435 440 445 Ile Gln Pro Arg Val Leu Phe Met Thr Asn Pro Asp Asp Gly Gln Gly 450 455 460 Asn Leu Tyr Ala Glu Ile Leu Arg Ala Gly Val Glu Gln Leu Cys Arg 465 470 475 480 Val Trp Arg His Glu Ser Gln Leu Asp Tyr Thr Arg Leu Ala Gln Met 485 490 495 Asp Ala His Pro Pro His Ile Arg Pro Val Trp Ala Asn Gln Leu Val 500 505 510 Arg Phe Ala Asn Asn Glu Gln Glu Asn Leu Glu Tyr Cys Cys Ala Trp 515 520 525 Val Ala Lys Ile Leu Leu Ser Glu Arg Thr Ile Glu Glu Leu Asn Leu 530 535 540 Tyr Leu Pro Arg Gln Ile 545 550 <210> 138 <211> 271 <212> PRT <213> HPDHps <400> 138 Met Ser Tyr Gly Ser Lys Ala Ala Glu Arg Leu Ala Asn Lys Val Ile 1 5 10 15 Leu Ile Thr Gly Ala Ser Ala Ala Ala Gly Ile Gly Ala Ala Thr Ala 20 25 30 Arg Glu Phe Ala Glu Ala Ala His Gly Asn Ile Lys Leu Ile Leu Thr 35 40 45 Ala Arg Arg Lys Asp Lys Leu Asp Ala Leu Ala Lys Glu Leu Thr Asp 50 55 60 Lys Tyr Asp Asn Ile Lys Val His Thr Val Ala Leu Asp Val Thr Lys 65 70 75 80 Leu Glu Ser Ile Gly Gln Phe Val Lys Asp Leu Pro Gln Glu Phe Ala 85 90 95 Asp Ile Asp Val Leu Val Asn Asn Ala Gly Leu Ala Leu Gly Arg Asp 100 105 110 Pro Ile Gly Glu Ile Leu Pro Glu Asp Ile Gln Gly Met Phe Gln Thr 115 120 125 Asn Val Leu Gly Leu Ile Thr Leu Thr Gln Ala Val Leu Pro Ile Phe 130 135 140 Lys Ala Lys Asn Ser Gly Asp Ile Val Asn Leu Gly Ser Ile Ala Gly 145 150 155 160 Arg Asp Pro Tyr Pro Gly Gly Gly Ile Tyr Cys Pro Thr Lys Ala Ala 165 170 175 Val Lys Ser Phe Ser His Val Leu Arg Lys Glu Leu Ile Asn Thr Arg 180 185 190 Ile Arg Val Leu Glu Val Asp Pro Gly Asn Val Glu Thr Glu Phe Ser 195 200 205 Ile Thr Arg Phe Lys Asn Asp Val Asp Lys Ala Lys Ala Val Tyr Ala 210 215 220 Asn Thr Glu Pro Leu Val Ala Glu Asp Ile Ala Glu Val Ile Val Phe 225 230 235 240 Gly Ile Thr Arg Lys Gln Asn Thr Val Ile Ala Glu Thr Leu Ile Phe 245 250 255 Ser Thr Asn Gln Ala Ser Ala Ser His Leu Tyr Lys Gly Gln Val 260 265 270 <210> 139 <211> 249 <212> PRT <213> HPDHra <400> 139 Met Ile Ile Phe Val Thr Gly Ala Thr Ala Gly Phe Gly Ala Glu Ile 1 5 10 15 Ala Arg Lys Phe Val Lys Glu Gly His Lys Val Ile Ala Thr Gly Arg 20 25 30 Arg Lys Glu Arg Leu Asp Asp Leu Lys Ala Glu Leu Gly Asp Ala Leu 35 40 45 Tyr Ala Val Glu Leu Asp Val Thr Asn Arg Ala Ser Ile Glu Glu Ala 50 55 60 Ile Ala Ser Leu Pro Ala Asp Trp Gln Asn Ile Asp Val Leu Val Asn 65 70 75 80 Asn Ala Gly Leu Ala Leu Gly Leu Glu Lys Ala Asp Lys Ala Ser Leu 85 90 95 Glu Asp Trp Asp Lys Met Ile Asp Thr Asn Thr Lys Gly Leu Val Tyr 100 105 110 Met Thr Arg Ala Val Leu Pro Gly Met Val Glu Arg Asn Thr Gly His 115 120 125 Val Ile Asn Ile Gly Ser Thr Ala Gly Asn Trp Pro Tyr Ala Gly Gly 130 135 140 Asn Val Tyr Gly Ala Thr Lys Ala Phe Val Arg Gln Phe Ser Leu Asn 145 150 155 160 Leu Arg Thr Asp Leu His Gly Thr His Val Arg Val Thr Asn Ile Glu 165 170 175 Pro Gly Leu Cys Gly Gly Thr Glu Phe Ser Asn Val Arg Phe Lys Gly 180 185 190 Asp Asp Glu Lys Val Ser Lys Thr Tyr Asp Asn Thr Asn Pro Leu Thr 195 200 205 Ala Ala Asp Val Ala Glu Ser Val Phe Trp Ala Ser Thr Leu Pro Ala 210 215 220 His Val Asn Ile Asn Ser Ile Glu Met Met Pro Val Ser Gln Ser Leu 225 230 235 240 Ala Gly Leu Gln Val His Arg Glu Gly 245 <210> 140 <211> 550 <212> PRT <213> HPDHrc <400> 140 Met Ser Thr Val Arg Arg Leu Glu Gly Lys Val Ala Leu Ile Thr Gly 1 5 10 15 Gly Ala Gly Asn Ile Gly Glu Val Ile Thr Arg Arg Phe Leu Ala Glu 20 25 30 Gly Ala Thr Val Val Ile Thr Gly Arg Asn Ala Glu Lys Leu Ala Val 35 40 45 Tyr Arg Arg Arg Leu Ile Asp Glu Glu Arg Val Ala Pro Glu Arg Val 50 55 60 Val Ala Leu Arg Met Asp Gly Ser Asp Ile Ala Gln Val Arg Ala Gly 65 70 75 80 Val Ala Gln Ile Val His Gly Gly Thr Asp Val Pro Ile Pro Leu His 85 90 95 Arg Ile Asp Ile Leu Val Asn Asn Ala Gly Ser Ala Gly Pro Arg Arg 100 105 110 Arg Leu Val Asp Ile Pro Leu Glu Pro Ser Glu Val Gln Pro Pro Asp 115 120 125 Ser Glu Thr Leu Ala Gln Ala Val Gly Asn Leu Val Gly Ile Thr Trp 130 135 140 Asn Leu Thr Arg Ala Ala Ala Pro His Met Pro Ser Gly Ser Ser Val 145 150 155 160 Ile Asn Ile Ser Thr Ile Phe Ser Arg Thr Asp Tyr Tyr Gly Arg Ile 165 170 175 Ala Tyr Val Ala Pro Lys Ala Ala Leu Asn Ala Leu Ser Asp Gly Leu 180 185 190 Ala Arg Glu Leu Gly Val Arg Gly Ile Arg Val Asn Thr Ile Tyr Pro 195 200 205 Gly Pro Ile Glu Ser Glu Arg Ile Tyr Thr Met Phe Gln Ala Met Asp 210 215 220 Ala Leu Lys Gly Gln Pro Glu Gly Asp Thr Ala Ser Gly Phe Leu Arg 225 230 235 240 Met Met Arg Leu Ser Arg Ile Asp Gln Asn Gly Glu Val Val Lys Arg 245 250 255 Phe Pro Ser Pro Val Asp Val Ala Asn Thr Ala Val Phe Leu Ala Ser 260 265 270 Asp Glu Ser Ala Ala Phe Thr Gly His Ala Phe Glu Val Thr His Gly 275 280 285 Met Glu Val Pro Thr Glu Ser Arg Thr Thr Phe Val Ser Arg Pro Gly 290 295 300 Leu Arg Ser Val Asp Ala Thr Gly Lys Val Ile Leu Ile Cys Ala Gly 305 310 315 320 Asp Gln Val Asp Asp Ala Val Ala Leu Ala Asp Thr Leu Arg Ser Cys 325 330 335 Arg Ala Thr Val Val Ile Gly Phe Arg Asp Pro Arg Ala Leu Glu Lys 340 345 350 Ala Ser Val Leu Leu Arg Glu Pro Arg His Ala Leu Ala Ala Asp Met 355 360 365 Tyr Gly Arg Pro Thr Met Thr Ala Glu Ala Arg Leu Val Arg Leu Asp 370 375 380 Pro Leu Asp Pro Arg Ala Ala Ala Gln Thr Leu Glu Gln Ile His Ala 385 390 395 400 Glu Leu Gly Ala Ile His His Ala Val Val Leu Pro Gly Gln Ser Arg 405 410 415 His Ala Pro Ser Ala Ser Leu Ile Glu Val Asp Asp Gln Val Val Glu 420 425 430 Arg Phe Leu His Gln Glu Leu Val Gly Thr Ile Ala Leu Ala Arg Glu 435 440 445 Leu Ala Arg Phe Trp Glu Glu Tyr Pro Ser Gly Ser Ser Met His Arg 450 455 460 Val Leu Phe Val Ser Asn Pro Asp Asp Gln Gln Gly Asn Gln Tyr Ser 465 470 475 480 His Ile Leu Arg Ala Ala Val Glu Gln Leu Val Arg Val Trp Arg His 485 490 495 Glu Ser Glu Tyr Asp Ser Val Asn Pro Ala His Gln Gln Glu Gly Gln 500 505 510 Ser Ser Ala Ala Val Trp Ala Asn Gln Leu Ile Arg Tyr Val Asn Asn 515 520 525 Glu Met Ala Asn Leu Asp Phe Thr Cys Ala Trp Val Ala Lys Leu Leu 530 535 540 Gly Ser Asp Arg Arg Ile 545 550 <210> 141 <211> 322 <212> PRT <213> HPDHsi <400> 141 Met Phe Ile His Met Lys Ser Ile Asn Lys Val Ala Val Val Gly Ala 1 5 10 15 Gly Ile Ile Gly Val Gly Trp Thr Thr Leu Leu Leu Thr Lys Gly Tyr 20 25 30 Lys Val Asn Leu Tyr Thr Glu Lys Lys Glu Thr Leu Glu Lys Gly Leu 35 40 45 Ala Lys Val Ser Ala Tyr Leu Val Asn Leu Lys Asn Leu Arg Met Ile 50 55 60 Asp Gly Glu Pro Ile Asp Tyr Gln Arg Asn Leu Thr Gly Thr Thr Lys 65 70 75 80 Ile Asp Glu Ala Ile Gln Asn Val Glu Phe Val Ile Glu Ala Ile Ile 85 90 95 Glu Asp Tyr Ser Ala Lys Lys Asn Leu Phe Asn Tyr Leu Asp Ser Gln 100 105 110 Leu Pro Arg Asp Ile Ile Ile Ala Ser Ser Thr Ser Gly Leu Leu Met 115 120 125 Thr Glu Ile Gln Lys Ala Met Thr Arg Tyr Pro Asp Arg Gly Val Ile 130 135 140 Ala His Pro Trp Asn Pro Pro His Leu Leu Pro Leu Val Glu Ile Val 145 150 155 160 Pro Gly Asp Lys Thr Ser Lys Tyr Thr Leu Asp Val Thr Lys Asp Phe 165 170 175 Met Glu Lys Leu Asp Arg Val Val Val Val Leu Lys Lys Glu Val Pro 180 185 190 Gly Phe Ile Gly Asn Arg Leu Ala Phe Ala Leu Phe Arg Glu Ala Val 195 200 205 Asn Leu Ile Asp Glu Gly Val Ala Thr Val Glu Asp Ile Asp Lys Val 210 215 220 Met Thr Ala Ala Ile Gly Leu Arg Trp Ala Phe Met Gly Pro Phe Leu 225 230 235 240 Thr Tyr His Leu Gly Gly Gly Glu Gly Gly Ile Glu Tyr Phe Phe Ser 245 250 255 Lys Gly Phe Gly Tyr Gly Ala Asn Glu Trp Met Tyr Thr Leu Ala Lys 260 265 270 Trp Asp Lys Phe Pro Tyr Thr Ala Val Lys Lys Ile Val Asp Gln Met 275 280 285 Lys Glu Tyr Glu Phe Ile Lys Gly Lys Asn Phe Gln Asp Leu Ser Arg 290 295 300 Trp Arg Asp Glu Thr Leu Val Arg Val Tyr Lys Ala Val Phe Gly Glu 305 310 315 320 Lys Arg <210> 142 <211> 550 <212> PRT <213> HPDHsl <400> 142 Met Asn Ala Met Gly Lys Thr Glu Thr Gln Gly Arg Leu Ala Gly Lys 1 5 10 15 Val Ala Leu Ile Thr Gly Ala Ala Gly Asn Leu Gly Gly Ser Ile Val 20 25 30 Arg Arg Tyr Leu Ala Glu Gly Ala Thr Val Val Met Ser Gly Arg Asp 35 40 45 Ala Ala Arg Thr Gln Ala Ala Leu Asp Ala Ala Val Ala Asp Val Gly 50 55 60 Gly Thr Ala Ser Ile Val Thr Met Asp Gly Ala Asn Ile Glu Ser Val 65 70 75 80 Arg Ala Gly Ile Ala Asp Val Val Ala Arg His Gly Arg Ile Asp Val 85 90 95 Leu Val Asn Asn Ala Gly Ser Ala Gly Pro Lys Gln Val Leu Gly Asp 100 105 110 Leu Pro Leu Leu Pro Glu Glu Leu Glu Gly Thr Pro Asp Ser Glu Thr 115 120 125 Val Gly Asp Ala Ala Arg Asn Ile Met Val Val Ala Trp Asn Leu Val 130 135 140 Arg Ala Ala Ala Ser Ala Met Gly Glu Gly Gly Ser Ile Ile Asn Val 145 150 155 160 Ser Thr Ile Phe Ser Arg Thr Asp Tyr Phe Gly Arg Ala Ala Tyr Thr 165 170 175 Val Pro Lys Ala Ala Leu Asn Ile Leu Ser Lys Gly Ile Ala Arg Glu 180 185 190 Leu Gly Thr Arg Gly Ile Arg Val Asn Gln Leu Tyr Pro Gly Pro Ile 195 200 205 Ala Ser Glu Arg Ile Arg Thr Val Phe Ala Ala Met Asp Lys Leu Lys 210 215 220 Gly Asp Asp Ala Gly Ser Thr Ala Asn His Phe Phe Asn Leu Met Ala 225 230 235 240 Leu Glu Arg Ser Val Gly Glu Asp Ala Lys Ala Lys Thr Phe Pro Thr 245 250 255 Pro Ala Asp Ile Ala Asn Thr Cys Val Phe Leu Gly Ser Asp Glu Ser 260 265 270 Ala Ala Phe Ala Ala His Asp Phe Glu Val Thr His Gly Met Ala Val 275 280 285 Pro Leu Glu Ser Lys Ser Glu Phe Leu His Arg Pro Asp Leu Arg Ala 290 295 300 Ile Asp Gly Asp Gly His Gly Ile Leu Ile Ser Ala Gly Asn Gln Leu 305 310 315 320 Asp Asp Ala Leu Ala Met Ala Gly Leu Phe Ala Ala Gln Gly Ser Lys 325 330 335 Val Leu Leu Gly Phe His Ala Gln Ala Ala Leu Asp Asp Ala Ala Glu 340 345 350 Arg Leu Pro Ala Gly Val Lys Ala Val Arg Phe Pro Arg His Asp His 355 360 365 Met Ala Met Asp Ala Glu Leu Ala Arg Phe Thr Ala His Val Ala Ala 370 375 380 Ile Asp Gly Ala Ile Ile Leu Pro Thr Arg Gly Pro Gly Tyr Phe His 385 390 395 400 Gly Ala Leu Asn Glu Ala Ser Asp Ala Ala Val Glu Arg Phe Val Asp 405 410 415 Asp Glu Leu Glu Gly Ala Ile Ala Val Ala Arg Thr Leu Asp Arg Tyr 420 425 430 Trp Gln Gln Gly Gln Asp Leu Pro Gly Asp Pro Arg Val Leu Phe Ile 435 440 445 Thr Asn Pro Asp Asp Gly Ala Gly Asn Gly Trp Ala Asp Val Gly Arg 450 455 460 Ala Ala Ile Glu Glu Leu Leu Arg Val Trp Arg Asp Glu Ala Arg Val 465 470 475 480 Ala Ala Thr Gln Gly Lys Thr Pro Arg Pro Val Trp Gly Asn Gln Ile 485 490 495 Val Arg Phe Gly Asn Asn Glu Pro Glu Asn Arg Asp Phe Ala Ala Gly 500 505 510 His Ala Ala Arg Leu Leu Leu Gly Gly Arg Gln Ile Glu Pro Ile Asn 515 520 525 Leu Tyr Ile Pro Ala Ser Ile Thr Lys Ala Thr Gly Ala Arg Arg Ala 530 535 540 Leu Ala Gly Thr Ala Glu 545 550 <210> 143 <211> 288 <212> PRT <213> HPDHsm <400> 143 Met Ala Ile Leu Ile Thr Gly Ala Ser Ser Gly Phe Gly Ala Ala Met 1 5 10 15 Ala Thr Ala Phe Val Gln Ala Gly Tyr Leu Val Ile Gly Thr Ala Arg 20 25 30 Arg Thr Glu Lys Leu Ala Asp Leu His Ala Lys Leu Gly Asp Arg Phe 35 40 45 Gln Pro Ile Thr Met Asp Met Thr Ser Lys Asp Asp Ile Gln Ala Ala 50 55 60 Leu Ala His Ile Gln Thr Leu Pro Glu Tyr Phe Arg Glu Ile Asp Cys 65 70 75 80 Leu Ile Asn Asn Ala Gly Leu Ala Leu Gly Leu Asp Arg Ala Asp Gln 85 90 95 Ala Asp Phe Thr Asp Trp Glu Thr Met Ile Gln Thr Asn Ile Ile Gly 100 105 110 Leu Thr Tyr Leu Thr Arg Gln Ile Leu Pro Lys Met Val Glu Asn Lys 115 120 125 Arg Gly Tyr Ile Ile Asn Met Gly Ser Ile Ala Gly Asn Tyr Pro Tyr 130 135 140 Ala Gly Ser Asn Val Tyr Gly Ala Thr Lys Ala Tyr Val Arg Gln Phe 145 150 155 160 Ser Leu Asn Leu Arg Ala Asp Leu His Gly Thr Gly Val Arg Val Thr 165 170 175 Asn Ile Glu Pro Gly Leu Val Glu Gly Ser Glu Phe Ser Asn Val Arg 180 185 190 Phe Lys Gly Asp Asp Lys Arg Ala Ala Gln Leu Tyr Glu Tyr Ala Asn 195 200 205 Ala Leu Thr Ser Glu Asp Ile Ala Gln Thr Ala Leu Trp Leu Tyr Gln 210 215 220 Arg Pro Ala His Met Asn Val Asn Thr Ile Glu Leu Met Pro Thr Thr 225 230 235 240 Gln Ser Trp Ala Gly Met Gln Val Tyr Lys Asp Thr Pro Pro Pro Pro 245 250 255 Pro Pro Val Val Glu Pro Lys Pro Ala Asp Phe Ala Pro Gln Pro Ala 260 265 270 Pro Gln Glu Glu Ser Gly Phe Phe Ser Lys Ile Thr Ser Leu Phe Lys 275 280 285 <210> 144 <211> 318 <212> PRT <213> HPDHst <400> 144 Met Arg Gln Ile Lys Thr Val Ser Val Ile Gly Ala Gly Val Ile Gly 1 5 10 15 Ala Gly Trp Ser Thr Leu Leu Ala Leu Lys Gly Tyr Glu Asn Trp Phe 20 25 30 Tyr Thr Glu Lys Lys Glu Thr Leu Asp Lys Gly Leu Ala Lys Ile Lys 35 40 45 Gly Tyr Leu Asn Val Leu Tyr Glu Tyr Lys Leu Ile Asp Lys Glu Pro 50 55 60 Asp Tyr Tyr Met Gln Arg Ile His Pro Thr Thr Lys Leu Asp Glu Ala 65 70 75 80 Ile Ser Asn Thr Asp Phe Val Leu Glu Ala Ile Val Glu Glu Tyr Gly 85 90 95 Ala Lys Lys Ala Leu Phe Lys Gln Leu Asp Glu Lys Leu Asp Lys Asp 100 105 110 Val Ile Leu Ala Ser Ser Thr Ser Gly Leu Leu Met Thr Glu Ile Gln 115 120 125 Lys Ala Met Thr Lys Tyr Pro Glu Arg Gly Ile Ile Ala His Pro Trp 130 135 140 Asn Pro Pro His Leu Leu Pro Leu Val Glu Ile Val Pro Gly Glu Lys 145 150 155 160 Thr Ser Gln Asp Thr Ile Tyr Ala Thr Lys Asp Phe Met Glu Asn Lys 165 170 175 Leu Glu Arg Val Val Val Val Leu Lys Lys Glu Ile Ser Gly Phe Ile 180 185 190 Gly Asn Arg Leu Ala Phe Ala Leu Phe Arg Glu Ala Val Tyr Leu Val 195 200 205 Asp Glu Gly Ile Ala Thr Val Glu Asp Ile Asp Lys Val Met Thr Ala 210 215 220 Ala Ile Gly Leu Arg Trp Ala Phe Met Gly Pro Phe Leu Thr Tyr His 225 230 235 240 Leu Gly Gly Gly Glu Gly Gly Leu Glu Tyr Phe Phe Ser Arg Gly Phe 245 250 255 Gly Tyr Gly Ala Asn Glu Trp Met His Thr Leu Ala Lys Tyr Asp Lys 260 265 270 Phe Pro Tyr Thr Gly Val Val Lys Ser Val Gln Gln Met Lys Glu Tyr 275 280 285 Glu Phe Val Lys Ser Lys Thr Phe Gln Glu Leu Ser Arg Trp Arg Asp 290 295 300 Glu Lys Leu Ile Ser Leu Ile Lys Leu Leu Arg Gly Lys Ile 305 310 315

Claims (13)

  1. AADH를 코딩하는 외인성 유전자; ACC를 코딩하는 내인성 또는 외인성 유전자; MCR을 코딩하는 외인성 유전자; 및 HPDH를 코딩하는 외인성 유전자를 함유하고, [피루브산 → 아세트알데히드 → 아세틸-CoA → 말로닐-CoA → 말로네이트 세미알데히드 → 3-HP]의 생합성 경로를 통해 3-HP를 생산할 수 있는 재조합 효모.
  2. 제1항에 있어서,
    상기 AADH를 코딩하는 유전자는 표 1 내지 3에 기재된 AADH 아미노산 서열들로 구성된 군에서 선택되는 AADH 아미노산 서열과 80% 이상의 상동성을 가지는 아미노산 서열의 AADH를 인코딩하는 핵산인 것을 특징으로 하는 재조합 효모.
  3. 제1항에 있어서,
    상기 ACC를 코딩하는 유전자는 표 4에 기재된 ACC 아미노산 서열들로 구성된 군에서 선택되는 ACC 아미노산 서열과 80% 이상의 상동성을 가지는 아미노산 서열의 ACC를 인코딩하는 핵산인 것을 특징으로 하는 재조합 효모.
  4. 제1항에 있어서,
    상기 MCR은 말로닐-CoA를 말로네이트 세미알데히드로 전환하는 기능과 말로네이트 세미알데히드를 3-HP로 전환하는 기능을 동시에 가지는 2기능성(bi-functional)인 것을 특징으로 하는 재조합 효모.
  5. 제4항에 있어서,
    상기 2기능성 MCR을 코딩하는 유전자는 표 5에 기재된 MCR 아미노산 서열들로 구성된 군에서 선택되는 MCR 아미노산 서열과 80% 이상의 상동성을 가지는 아미노산 서열의 2기능성 MCR을 인코딩하는 핵산인 것을 특징으로 하는 재조합 효모.
  6. 제1항에 있어서,
    상기 MCR은 말로닐-CoA를 말로네이트 세미알데히드로 전환하는 단일기능성(mono-functional)이고, 말로네이트 세미알데히드를 3-HP로 전환시킬 수 있는 효소를 코딩하는 유전자를 추가로 함유하는 것을 특징으로 하는 재조합 효모.
  7. 제6항에 있어서,
    상기 단일기능성 MCR을 코딩하는 유전자는 표 6에 기재된 MCR 아미노산 서열들로 구성된 군에서 선택되는 MCR 아미노산 서열과 80% 이상의 상동성을 가지는 아미노산 서열의 단일기능성 MCR을 인코딩하는 핵산인 것을 특징으로 하는 재조합 효모.
  8. 제6항에 있어서,
    상기 말로네이트 세미알데히드를 3-HP로 전환시킬 수 있는 효소를 코딩하는 유전자는 표 7 내지 표 10에 기재된 HPDH 아미노산 서열들, HIBADH 아미노산 서열들, HBDH 아미노산 서열들 및 BDH 아미노산 서열들로 구성된 군에서 선택되는 HPDH 아미노산 서열, HIBADH 아미노산 서열, HBDH 아미노산 서열 또는 BDH 아미노산 서열과 80% 이상의 상동성을 가지는 아미노산 서열의 효소를 인코딩하는 핵산인 것을 특징으로 하는 재조합 효모.
  9. 제1항에 있어서,
    내산성을 가지는 것을 특징으로 하는 재조합 효모.
  10. 제9항에 있어서,
    사카로마이세스 세레비지애 (Saccharomyces cerevisiae), 카자크스타니아 엑시구아 (Kazachstania exigua), 카자크스타니아 불데리 (Kazachstania bulderi), 및 칸디다 휴밀리스 (Candida humilis)로 구성된 군에서 선택된 것을 특징으로 하는 재조합 효모.
  11. 다음 단계를 포함하는 3-HP의 제조방법:
    (a) 제1항 내지 제10항 중 어느 한 항의 재조합 효모를 적어도 하나의 탄소원을 함유하는 배지에서 배양하여, 3-HP를 생성시키는 단계; 및
    (b) 상기 생성된 3-HP를 회수하는 단계.
  12. 제11항에 있어서,
    상기 탄소원은 글루코즈, 자일로즈, 아라비노즈, 수크로즈, 프룩토즈, 셀룰로오즈, 갈락토오스, 글루코즈 올리고머 및 글리세롤로 구성된 군에서 선택된 하나 이상인 것을 특징으로 하는 3-HP의 제조방법.
  13. 제11항에 있어서,
    상기 배양은 pH 2.6~4.0에서 수행하는 것을 특징으로 하는 3-HP의 제조방법.
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US20210324346A1 (en) * 2020-04-17 2021-10-21 Sk Innovation Co., Ltd. Recombinant acid-resistant yeast with suppressed glycerol production and method of producing lactic acid using the same
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