CN107406818A - 增强产油酵母中核心脂质的生产 - Google Patents
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Abstract
本发明公开了包含一种或更多种例如相对于相同类型的未经修饰细胞提高细胞的脂质含量的遗传修饰的转化细胞。本发明还公开通过提高细胞中一种或更多种蛋白质的活性和/或通过降低细胞中一种或更多种蛋白质的活性来提高细胞脂质含量的方法。
Description
相关申请
本申请要求于2014年12月30日提交的美国临时专利申请序列号62/097,781的优先权权益。
序列表
本申请包含序列表，其已经以ASCII格式电子提交并且在此通过引用整体并入本文。于2015年12月11日创建的所述ASCII副本命名为NGX03825SL.txt并且大小为575,737字节。
背景技术
脂质是食品和化妆品工业中不可或缺的成分，并且其是生物柴油和生化工业中的重要前体。许多产油微生物产生脂质，包括已良好表征的酵母，解脂耶氏酵母(Yarrowialipolytica)。
产油微生物可容易地且有成本效益地大规模培养，这表明用于生物柴油和生物化学生产的广泛适用性。微生物还可被工程化成生产食品工业和饮料工业的高价值产品。此外，这些产品通常被隔离在微生物中，这可以有利于其分离和纯化。
微生物以不同的速率和不同的效率生产脂质产品。真核生物中的脂质产生通常如下进行：在线粒体中通过丙酮酸脱氢酶将丙酮酸氧化成乙酰CoA，并且随后通过代谢中间体柠檬酸将乙酰CoA输出到细胞溶质中。线粒体丙酮酸氧化和柠檬酸输出导致在线粒体中净累积还原型烟酰胺腺嘌呤二核苷酸(NADH)。然而，线粒体中NADH的累积对于脂质产生来说不是最佳的，部分地是因为线粒体NADH不能还原细胞溶质酮，这导致总脂质产率较低。
产油生物的脂质产率可以通过上调、下调或缺失参与脂质途径的基因来提高。然而，即便作最乐观的估计成功的酶调节不能被预测。例如，在解脂耶氏酵母中过表达来自高山被孢霉属(Mortierella alpine)的2型二酰基甘油酰基转移酶对脂质含量没有显著作用(美国专利号7,198,937；其在此通过引用并入)。
发明内容
在一些方面，本发明涉及一种转化细胞，其包含第一遗传修饰和第二遗传修饰，其中所述第一遗传修饰提高细胞中磷酸转酮酶蛋白质的活性，并且所述第二遗传修饰提高细胞中磷酸乙酰转移酶蛋白质的活性。
在一些实施方案中，本发明涉及一种转化细胞，其包含第一遗传修饰、第二遗传修饰、第三遗传修饰和第四遗传修饰，其中所述第一遗传修饰提高细胞中磷酸转酮酶蛋白质的活性，所述第二遗传修饰提高细胞中磷酸乙酰转移酶蛋白质的活性，所述第三遗传修饰提高细胞中果糖-1,6-二磷酸酶蛋白质的活性，并且所述第四遗传修饰降低细胞中磷酸果糖激酶蛋白质的活性。
在一些实施方案中，本发明涉及一种转化细胞，其包含第一遗传修饰、第二遗传修饰和第三遗传修饰，其中所述第一遗传修饰提高细胞中丙酮酸脱羧酶蛋白质的活性，所述第二遗传修饰提高细胞中磷酸乙酰转移酶蛋白质的活性，并且所述第三遗传修饰提高细胞中乙酸激酶的活性。
在一些实施方案中，本发明涉及一种转化细胞，其包含第一遗传修饰、第二遗传修饰和第三遗传修饰，其中所述第一遗传修饰提高细胞中柠檬酸/草酰乙酸线粒体转运蛋白的活性，所述第二遗传修饰提高细胞中细胞溶质苹果酸酶蛋白质的活性，并且所述第三遗传修饰提高细胞中细胞溶质内丙酮酸羧化酶蛋白质的活性。
在一些实施方案中，本发明涉及一种转化细胞，其包含第一遗传修饰和第二遗传修饰，其中所述第一遗传修饰提高细胞中柠檬酸/草酰乙酸线粒体转运蛋白的活性，并且所述第二遗传修饰提高细胞的NADH特异性烯酰基酰基载体还原酶活性。
在一些方面，本发明涉及由本发明的转化细胞获得的产品。在一些实施方案中，所述产品包含油、脂质、脂肪酸、脂肪醇、三酰基甘油酯、类异戊二烯或法呢烯。所述产品可包含硬脂酸、油酸、亚油酸、癸酸、辛酸、己酸、月桂酸、豆蔻酸、棕榈酸、棕榈油酸或角鲨烯。例如，所述产品可以是油酸。
在一些方面，本发明涉及提高细胞的脂质含量的方法，其包括用编码磷酸转酮酶蛋白质的第一核苷酸序列转化所述细胞，以及用编码磷酸乙酰转移酶蛋白质的第二核苷酸序列转化所述细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码磷酸转酮酶蛋白质的第一核苷酸序列转化所述细胞，用编码磷酸乙酰转移酶蛋白质的第二核苷酸序列转化所述细胞，用编码果糖-1,6-二磷酸酶蛋白质的第三核苷酸序列编码所述细胞，以及用降低细胞中天然磷酸果糖激酶蛋白质的活性的第四核苷酸序列转化所述细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码丙酮酸脱羧酶蛋白质的第一核苷酸序列转化所述细胞，用编码磷酸乙酰转移酶蛋白质的第二核苷酸序列转化所述细胞，以及用编码乙酸激酶蛋白质的第三核苷酸序列转化所述细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码柠檬酸/草酰乙线粒体转运蛋白的第一核苷酸序列转化所述细胞，用编码细胞溶质苹果酸酶蛋白质的第二核苷酸序列转化所述细胞，以及用编码细胞溶质丙酮酸羧化酶蛋白质的第三核苷酸序列转化所述细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码柠檬酸/草酰乙酸线粒体转运蛋白的第一核苷酸序列转化所述细胞，以及用第二核苷酸序列转化所述细胞。第二核苷酸序列可以编码NADH特异性烯酰基酰基载体还原酶蛋白质，或者第二核苷酸序列可以能够与天然I型脂肪酸合酶烯酰还原酶基因中的核苷酸序列重组，并且用第二核苷酸序列转化细胞可提高细胞的NADH特异性烯酰基酰基载体还原酶活性。
转化细胞可选自由藻类、细菌、霉菌、真菌、植物和酵母组成的组。细胞可以是酵母。例如，细胞可以是选自由Arxula酵母(Arxula adeninivorans)、酿酒酵母属(Saccharomyces cerevisiae)和解脂耶氏酵母属组成的组的酵母。
参照下面的描述、附图和权利要求，将更好地理解本发明的这些及其他特征、方面和优点。
附图简述
图1示出了在未经修饰酵母中产生乙酰CoA和NADPH的途径。
图2示出了一条新的磷酸转酮酶途径。
图3示出了一条新的磷酸转酮酶途径。
图4示出了一条新的丙酮酸脱羧酶途径。
图5示出了一条新的柠檬酸输出物途径。
图6示出了一条新的柠檬酸输出物途径。
图7是用于在酵母中过表达来自枯草芽孢杆菌(Bacillus subtilis)的磷酸乙酰转移酶基因(SEQ ID NO：116)的pNC582构建体的图谱。“2μori”表示来自2μ环质粒的酵母复制起点；“pMB1ori”表示来自pBR322质粒的大肠杆菌(E.coli)pMB1复制起点；“AmpR”表示用作氨苄西林的选择标记的bla基因；“PR11”表示酿酒酵母(S.cerevisiae)FBA1启动子-822至-1；“NG4”表示用作用潮霉素的选择标记的大肠杆菌(Escherichia coli)hph基因；“TER6”表示停止后205bp的酿酒酵母FBA1终止子；“PR4”表示解脂耶氏酵母(Y.lipolytica)EXP1启动子-999至-1；“NG321”表示由Life Technologies合成的天然枯草芽孢杆菌枯草亚种168PTA cDNA(SEQ ID NO：116)；“TER1”表示停止后300bp的解脂耶氏酵母CYC1终止子；“Sc URA3”表示用于在酵母中选择的酿酒酵母URA3营养缺陷标记。
图8是显示通过将细胞提取物与乙酰CoA和DTNB一起孵育进行的测定在412nm的吸光度的图。标记为NG321、NG322、NG324、NG309、NG310和NG311的细胞提取物是来自以下酿酒酵母的细胞提取物：其各自包含可将乙酰CoA转化为CoA-SH，从而使CoA-SH与DTNB反应产生报告分子5-硫代-2-硝基苯甲酸酯的磷酸乙酰转移酶的基因。标记为NG306的细胞提取物是来自酿酒酵母的细胞提取物，包含来自里氏木霉(Trichoderma reesei)的磷酸转酮酶基因，其作为阴性对照。
图9是显示来自包含磷酸乙酰转移酶基因的酿酒酵母和阴性对照酿酒酵母菌株NS20的细胞提取物的磷酸乙酰转移酶比活性的图。
图10是显示来自包含磷酸乙酰转移酶基因的酿酒酵母的细胞提取物和阴性对照酿酒酵母菌株NS20的磷酸乙酰转移酶比活性的图。
图11是显示来自包含磷酸乙酰转移酶基因的Arxula酵母(Arxulaadeninivorans)的细胞提取物和各自包含磷酸转酮酶基因的阴性对照Arxula酵母(Arxulaadeninivorans)菌株(NG304和NG306)的磷酸乙酰转移酶比活性的图。
图12是显示来自包含磷酸乙酰转移酶基因的Arxula酵母(Arxulaadeninivorans)和阴性对照Arxula酵母(Arxula adeninivorans)菌株NS252的细胞提取物的磷酸乙酰转移酶比活性的图。
图13是显示来自包含磷酸乙酰转移酶基因的解脂耶氏酵母和阴性对照解脂耶氏酵母菌株NS18的细胞提取物的磷酸乙酰转移酶比活性的图。
图14是显示来自包含磷酸乙酰转移酶基因的解脂耶氏酵母的细胞提取物和阴性对照解脂耶氏酵母菌株NS18的磷酸乙酰转移酶比活性的图。
图15是用于扩增敲除盒SEQ ID NO：143和SEQ ID NO：144以缺失解脂耶氏酵母菌株NS18中PFK1基因的pNC468构建体的图谱。“2μori”表示来自2μ环质粒的酿酒酵母复制起点；“pMB1ori”表示来自pBR322质粒的大肠杆菌pMB1复制起点；“AmpR”表示用作氨苄西林的选择标记的bla基因；“PR22”表示酿酒酵母TEF1启动子-412至-1；“NG3”表示用作诺尔丝菌素的选择标记的诺尔斯链霉菌(Streptomyces noursei)Nat基因；“TER2”表示停止后275bp的酿酒酵母CYC1终止子；“PR1”表示解脂耶氏酵母TEF1启动子-406至-1；“NG341”表示由Genscript合成的单纯疱疹病毒TDK基因cDNA；“TER7”表示停止后400bp的解脂耶氏酵母TEF1终止子；“Sc URA3”表示用于在酵母中选择的酿酒酵母URA3营养缺陷标记。
图16由标记为(A)和(B)的两幅图组成。这两幅图示出了包含PCR产物的成像DNA凝胶，所述PCR产物来自对转化有对应于SEQ ID NO:143和SEQ ID NO:144的敲除盒的解脂耶氏酵母菌株进行的PCR。另外，分析了来自亲本解脂耶氏酵母菌株NS18的PCR产物作为对照。图(A)(上图)示出了由PCR获得的结果，所述PCR用用于评估解脂耶氏酵母菌菌落是否具有完整的PFK1基因的引物NP2784(SEQ ID NO：157)和NP2785(SEQ ID NO：158)进行。只有阴性对照NS18显示出对应于NP2784/NP2785PCR产物的条带。图(B)(底部)示出了由PCR获得的结果，所述PCR用用于评估解脂耶氏酵母菌落是否包含整合到解脂耶氏酵母基因组中PFK1基因座的敲除盒的引物NP2784(SEQ ID NO：157)和NP356(SEQ ID NO：152)进行。菌落1、2、3、5、7和8各自显示出对应于NP2784/NP356基因产物的条带。菌落1被命名为菌株NS807。
图17是解脂耶氏酵母菌株NS18(野生型菌株)和转化有对应于SEQ ID NO：143和SEQ ID NO：144的敲除盒的菌株NS807的图像。在包含以葡萄糖作为唯一碳源的基本培养基的平板上培养菌株。菌株NS18能够在葡萄糖上生长，而被工程化为缺失磷酸果糖激酶1的NS807菌株在葡萄糖上不生长。
图18是显示野生型解脂耶氏酵母菌株NS18和敲除菌株NS807的磷酸果糖激酶比活性的图。
发明详述
综述
在一些方面，本发明涉及在产油酵母例如解脂耶氏酵母和Arxula酵母(Arxulaadeninivorans)中产生脂质(例如中性脂质和三酰甘油)。在一些方面，本发明涉及一种转化细胞，其包含一种或更多种相对于相同类型的未经修饰细胞提高细胞中由葡萄糖的脂质产率的遗传修饰。在一些方面，本发明涉及如下提高细胞的脂质含量的方法：用一种或更多种编码相对于相同类型的未经修饰细胞提高细胞中由葡萄糖的脂质产率的蛋白质的核苷酸序列转化细胞。
在一些方面，本发明涉及一种转化细胞，其包含一个或更多个相对于相同类型的未经修饰细胞更高效地产生用于合成脂质分子的代谢前体的代谢工程化途径，所述代谢前体例如乙酰辅酶A(乙酰CoA)、还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)、NADH和三磷酸腺苷(ATP)。在一些方面，本发明涉及通过用一种或更多种核苷酸序列转化细胞来提高细胞的脂质含量的方法，所述核苷酸序列编码催化或以其他方式完成代谢途径中产生代谢前体(例如乙酰CoA、NADPH、NADH或ATP)的一个或更多个步骤的蛋白质。
在线粒体内的丙酮酸氧化和通过代谢中间体柠檬酸将乙酰CoA从线粒体输出到细胞溶质中导致在线粒体中净积累NADH(图1)。这种积累对于获得高细胞脂质含量是不利的，因为细胞溶质中必须有还原等同物(例如，NADH和NADPH)可供用于将乙酰基还原成生长脂肪酸链上的酰基。用于这种还原的优选电子载体是定位于细胞质的NADPH。在一些方面，本发明涉及一种转化细胞，其包含一种或更多种相对于相同类型的未经修饰细胞提高细胞中NADPH产生(例如，细胞溶质NADPH产生)的遗传修饰。在一些方面，本发明涉及如下提高细胞的脂质含量的方法：用一种或更多种编码相对于相同类型的未经修饰细胞提高细胞中NADPH产生(例如细胞溶质NADPH产生)的蛋白质的核苷酸序列转化细胞。在一些方面，本发明涉及转化细胞，其包含一种或更多种相对于相同类型的未经修饰细胞提高脂质产生中细胞溶质NADH和/或细胞溶质NADPH的利用的遗传修饰。在一些方面，本发明涉及通过用一种或更多种核苷酸序列转化细胞来提高细胞的脂质含量的方法，所述核苷酸序列编码提高脂质产生中细胞溶质NADH和/或细胞溶质NADPH的利用的蛋白质。
定义
本文中使用的没有数量词修饰的名词指一个/种或多于一个/种(即至少一个/种)。作为实例，“要素”是指一个要素或多于一个要素。
术语“活性”是指细胞执行功能的总能力。例如，降低细胞中酶的活性的遗传修饰可以降低细胞中酶的量或降低酶的效率。敲除通过降低细胞中蛋白质的量来降低蛋白质的活性。或者，基因的突变可以降低其蛋白质产物的效率，而对细胞中蛋白质的量具有很小影响。降低酶效率的突变可影响活性位点，例如通过改变一个或更多个活性位点残基；其可损害酶的动力学，例如通过空间阻挡底物或产物；其可影响蛋白质折叠或动力学，例如通过降低正确折叠的酶的比例；其可影响蛋白质定位，例如通过阻止蛋白质定位于脂质颗粒；或者其可影响蛋白质降解，例如通过添加一个或更多个蛋白质切割位点，或者通过添加一个或更多个靶向蛋白质以蛋白质水解的残基或者氨基酸序列。这些突变影响编码区。降低蛋白质活性的突变可能反而影响基因的转录或翻译。例如，增强子或启动子的突变可通过降低其表达来降低蛋白质的活性。突变或缺失基因的非编码部分(例如其内含子)也可以降低转录或翻译。另外，基因上游调节子的突变可影响其蛋白质产物的活性；例如，一种或更多种阻遏物的过度表达可降低蛋白质的活性，以及一种或更多种激活物的敲除或突变可类似地降低蛋白质的活性。
提高细胞中蛋白质的活性的遗传修饰可以增加细胞中蛋白质的量或提高蛋白质的效率(例如，酶的效率)。例如，遗传修饰可简单地将额外的蛋白质拷贝插入细胞中，使得额外拷贝被转录并翻译成额外的功能蛋白质。添加的基因可以是宿主生物体天然的或来自不同生物体。或者，突变或缺失基因的非编码部分(例如其内含子)也可提高翻译。可以通过添加引起更多转录的新启动子来改变天然基因。类似地，可向基因添加增强子以提高转录，或者可以从基因中突变或缺失沉默子以提高转录。对天然基因编码区的突变也可提高蛋白质的活性，例如通过产生不与抑制性蛋白质或分子相互作用的蛋白质变体。一种或更多种激活物的过表达可通过提高蛋白质的表达来提高蛋白质的活性，以及一种或更多种阻遏物的敲除或突变可类似地提高蛋白质的活性。
术语“生物活性部分”指小于全长氨基酸序列但表现出全长序列的至少一种活性的氨基酸序列。例如，磷酸转酮酶的生物活性部分可指具有将木酮糖-5-磷酸转化成甘油醛-3-磷酸的生物活性的磷酸转酮酶的一个或更多个结构域。蛋白质的生物活性部分包括这样的肽或多肽，其包含与该蛋白质的氨基酸序列足够相同或由该蛋白质的氨基酸序列衍生的氨基酸序列，例如SEQ ID NO:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、121、123或125所示的氨基酸序列，这些序列包含少于全长蛋白质的氨基酸但是表现出该蛋白质的至少一种活性。类似地，蛋白质的生物活性部分包括这样的肽或多肽，其包含与该蛋白质的氨基酸序列足够相同或由该蛋白质的氨基酸序列衍生的氨基酸序列，例如SEQ ID NO:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、121、123或125所示的氨基酸序列，这些序列包含少于全长蛋白质的氨基酸但是表现出该蛋白质的至少一种活性。蛋白质的生物活性部分可包含例如至少100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223、224、225、226、227、228、229、230、231、232、233、234、235、236、237、238、239、240、241、242、243、244、245、246、247、248、249、250、251、252、253、254、255、256、257、258、259、260、261、262、263、264、265、266、267、268、269、270、271、272、273、274、275、276、277、278、279、280、281、282、283、284、285、286、287、288、289、290、291、292、293、294、295、296、297、298、299、300、301、302、303、304、305、306、307、308、309、310、311、312、313、314、315、316、317、318、319、320、321、322、323、324、325、326、327、328、329、330、331、332、333、334、335、336、337、338、339、340、341、342、343、344、345、346、347、348、349、350、351、352、353、354、355、356、357、358、359、360、361、362、363、364、365、366、367、368、369、370、371、372、373、374、375、376、377、378、379、380、381、382、383、384、385、386、387、388、389、390、391、392、393、394、395、396、397、398、399、400、401、402、403、404、405、406、407、408、409、410、411、412、413、414、415、416、417、418、419、420、421、422、423、424、425、426、427、428、429、430、431、432、433、434、435、436、437、438、439、440、441、442、443、444、445、446、447、448、449、450、451、452、453、454、455、456、457、458、459、460、461、462、463、464、465、466、467、468、469、470、471、472、473、474、475、476、477、478、479、480、481、482、483、484、485、486、487、488、489、490、491、492、493、494、495、496、497、498、499、500、501、502、503、504、505、506、507、508、509、510、511、512、513、514、515、516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、620、621、622、623、624、625、626、627、628、629、630、631、632、633、634、635、636、637、638、639、640、641、642、643、644、645、646、647、648、649、650、651、652、653、654、655、656、657、658、659、660、661、662、663、664、665、666、667、668、669、670、671、672、673、674、675、676、677、678、679、680、681、682、683、684、685、686、687、688、689、690、691、692、693、694、695、696、697、698、699、700个或更多个氨基酸。通常来说，生物活性部分包含具有催化活性，例如用于产生脂肪酸生物合成途径中的分子的催化活性，或者具有转运蛋白活性，例如用于线粒体转运的转运蛋白活性的结构域或基序。蛋白质的生物活性部分包括与全长肽具有相同活性的蛋白质的部分以及活性高于背景的每个部分。例如，酶的生物活性部分相对于全长酶可具有0.1％、0.5％、1％、2％、3％、4％、5％、10％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％、99.6％、99.7％、99.8％、99.9％、100％、100.1％、100.2％、100.3％、100.4％、100.5％、100.6％、100.7％、100.8％、100.9％、101％、105％、110％、115％、120％、125％、130％、135％、140％、145％、150％、160％、170％、180％、190％、200％、220％、240％、260％、280％、300％、320％、340％、360％、380％、400％或更高活性。蛋白质的生物活性部分可以包括缺少将该蛋白质靶向至细胞隔室的结构域的蛋白质部分。磷酸乙酰转移酶蛋白质的生物活性部分可以是长度为例如310个氨基酸的多肽。
术语“结构域”指蛋白质的氨基酸序列的一部分，其能够独立于蛋白质的剩余部分而折叠成稳定的三维结构。
术语“药物”指这样的任何分子，其抑制细胞生长或增殖，从而为含有赋予药物抗性的基因的细胞提供选择优势。药物包括抗生素、抗微生物剂、毒素和杀虫剂。
“干重”和“干细胞重量”意指在相对不存在水的情况下确定的重量。例如，提及产油细胞包含按干重计特定百分比的特定组分意指该百分比是基于细胞在基本上所有水被去除后的重量来计算的。
术语“编码”指包含编码区、编码区的部分或其互补体的核酸。DNA和RNA二者都可编码基因。DNA和RNA二者都可编码蛋白质。
本文中使用的术语“酶”指可催化化学反应的蛋白质。
术语“外源的”指引入细胞中的任何物质。“外源核酸”是通过细胞膜进入细胞的核酸。外源核酸可包含细胞的天然基因组中存在的和/或细胞基因组中先前不存在的核苷酸序列。外源核酸包括外源基因。“外源基因”是已引入细胞中(例如，通过转化/转染)的编码用于RNA和/或蛋白质的表达的核酸，并且还称为“转基因”。包含外源基因的细胞可称为重组细胞，可向其中引入另外的外源基因。外源基因相对于被转化的细胞可来自相同或不同的物种。因此，相对于基因的内源拷贝，外源基因可以包括在细胞基因组中占据不同位置或处于不同控制下的天然基因。外源基因可在细胞中以多于一个拷贝存在。外源基因可作为插入到基因组(核或质体)中或作为附加体分子而维持在细胞中。
术语“表达”指细胞中核酸或氨基酸序列(例如肽、多肽或蛋白质)的量。基因的表达提高指该基因的转录提高。氨基酸序列、肽、多肽或蛋白质的表达提高指编码该氨基酸序列、肽、多肽或蛋白质的核酸的翻译提高。
本文中使用的术语“基因”可涵盖包含外显子的基因组序列，特别是编码参与特定活性的多肽序列的多核苷酸序列。该术语还涵盖不衍生自基因组序列的合成核酸。在某些实施方案中，基因缺少内含子，如其基于cDNA和蛋白质序列的已知DNA序列合成。在另一些实施方案中，基因是合成的非天然cDNA，其中密码子已基于密码子使用针对在解脂耶氏酵母中表达被优化。该术语还可包括含有上游、下游和/或内含子核苷酸序列的核酸分子。
术语“遗传修饰”指转化的结果。根据定义，每种转化都会导致遗传修饰。
本文中使用的术语“同源物”指(a)相对于所讨论的未经修饰蛋白质具有氨基酸替换、缺失和/或插入以及与其衍生自的未经修饰蛋白质具有类似生物活性和功能活性的肽、寡肽、多肽、蛋白质和酶；以及(b)编码具有与(a)中所述相同特征的肽、寡肽、多肽、蛋白质和酶的核酸。
“诱导型启动子”是响应于特定刺激而介导可操作连接的基因的转录的启动子。
术语“整合的”指作为插入到细胞基因组中，例如插入到染色体中，包括插入到质体基因组中而维持在细胞中的核酸。
“可操作连接”指两个核酸序列，例如控制序列(通常为启动子)和连接的序列(通常是编码蛋白质的序列，也称为编码序列)之间的功能性连接。如果启动子可介导基因的转录，则启动子与基因可操作连接。
术语“敲除突变”或“敲除”指阻止天然基因被转录并翻译成功能蛋白质的遗传修饰。
术语“天然的”指在转化事件之前细胞或亲本细胞的组成。“天然基因”指不是通过转化事件引入细胞中的编码蛋白质的核苷酸序列。“天然蛋白质”指由天然基因编码的氨基酸序列。
术语“核酸”指任何长度的核苷酸，即脱氧核糖核苷酸或核糖核苷酸或者其类似物的聚合物形式。多核苷酸可具有任何三维结构，并且可执行任何功能。以下是多核苷酸的非限制性实例：基因或基因片段的编码区或非编码区、由连锁分析限定的基因座、外显子、内含子、信使RNA(mRNA)、转移RNA、核糖体RNA、核酶、cDNA、重组多核苷酸、支化多核苷酸、质粒、载体、任何序列的分离的DNA、任何序列的分离的RNA、核酸探针和引物。多核苷酸可包含经修饰的核苷酸，例如甲基化核苷酸和核苷酸类似物。如果存在的话，则可在聚合物组装之前或之后赋予对核苷酸结构的修饰。可进一步修饰多核苷酸，例如通过与标记组分缀合。在本文中提供的所有核酸序列中，U核苷酸可与T核苷酸互换。
首字母缩略词“ORF”代表开放阅读框。
术语“亲本细胞”指细胞从其传代的每种细胞。细胞的基因组由亲本细胞的基因组和随后对亲本细胞基因组的任何遗传修饰组成。
本文中使用的术语“质粒”是指与生物体的基因组DNA物理分离的环状DNA分子。质粒在被引入宿主细胞之前可被线性化(本文中称为线性化质粒)。线性化质粒不能自我复制，但是可整合到生物体的基因组DNA中并随其复制。
术语“部分”指肽、寡肽、多肽、蛋白质结构域和蛋白质。编码“蛋白质的部分”的核苷酸序列包括可被转录和/或翻译的核苷酸序列以及必须进行一个或更多个重组事件以被转录和/或翻译的核苷酸序列两种。例如，核酸可包含一个或更多个编码可选择标记蛋白质的氨基酸的核苷酸序列。这种核酸可被工程化以与编码蛋白质剩余部分的一个或更多个不同核苷酸序列重组。这样的核酸可用于产生敲除突变，因为仅与靶序列重组可重构全长可选择标记基因，而随机整合事件不太可能产生可产生功能性标记蛋白质的核苷酸序列。
“启动子”指导核酸转录的核酸控制序列。本文中使用的启动子包括在转录起始位点附近的必需核酸序列。启动子还任选地包括远端增强子或阻遏物元件，其可以位于距转录起始位点多达数千个碱基对。
术语“蛋白质”指包含氨基酸序列的分子，其中氨基酸通过肽键连接。
“重组的”指由于引入外源核酸或改变天然核酸而已经被修饰的细胞、核酸、蛋白质或载体。因此，例如，重组细胞可表达细胞的天然(非重组)形式中未发现的基因或者表达与非重组细胞表达的那些基因不同的天然基因。重组细胞可以但不限于包含编码基因产物或降低细胞中活性基因产物的水平的抑制元件例如突变、敲除、反义、干扰RNA(RNAi)或dsRNA的重组核酸。“重组核酸”是最初在体外，一般通过操作核酸，例如使用聚合酶、连接酶、外切核酸酶和内切核酸酶操作核酸形成的核酸，或者另外地是在自然界中通常不存在的形式。可以产生重组核酸例如以将两个或更多个核酸设置成可操作连接。因此，出于本发明的目的，分离的核酸或者通过连接天然通常不连接的DNA分子体外形成的表达载体都被认为是重组的。一旦重组核酸得以制备并引入宿主细胞或生物体中，其可使用宿主细胞的体内细胞机制进行复制；然而，出于本发明的目的。这样的核酸一旦重组产生尽管后续在细胞内复制但是仍然被认为是重组的。类似地，“重组蛋白质”是使用重组技术，即通过表达重组核酸制备的蛋白质。
术语“调控区”指影响基因的转录或翻译但不编码氨基酸序列的核苷酸序列。调控区包括启动子、操纵子、增强子和沉默子。
术语“基本上相同的”指编码蛋白质的生物活性部分的核苷酸或氨基酸序列，其与参照序列具有70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高的序列同一性。对于酶，基本上相同的序列通常保留参照序列的酶活性。例如，如果序列编码具有参照酶的10％至1,000％酶活性的酶，则其与该参照序列基本相同。
“转化”指将核酸转移到宿主生物体或宿主生物体的基因组中，实现遗传上稳定的遗传。将含有转化的核酸片段的宿主生物体称为“重组”、“转基因”或“转化”生物体。因此，本发明的分离的多核苷酸可以并入能够引入宿主细胞中并在宿主细胞中复制的重组构建体，通常为DNA构建体。这样的构建体可以是包含复制系统和能够在给定宿主细胞中转录和翻译多肽编码序列的序列的载体。通常来说，表达载体包含例如在5’和3’调控序列的转录控制之下的一个或更多个克隆基因和可选择标记。这样的载体还可以含有启动子调控区(例如，控制诱导型或组成型、环境或发育调节的、或位置特异性表达的调控区)、转录起始开始位点、核糖体结合位点、转录终止位点和/或多聚腺苷酸化信号。
术语“转化细胞”指已经进行转化的细胞。因此，转化细胞包含亲本基因组和可遗传的遗传修饰。
术语“三酰基甘油酯”、“三酰甘油”、“甘油三酯”和“TAG”是由甘油和三个脂肪酸构成的酯。
术语“载体”指通过其核酸可以在生物体、细胞或细胞组分之间增殖和/或转移的工具。载体包括质粒、线性DNA片段、病毒、噬菌体、前病毒、噬菌粒、转座子和人工染色体等，其可以或不可以能够自主地复制或整合到宿主细胞的染色体中。
微生物工程
A.概述
在本发明的某些实施方案中，对微生物进行遗传修饰以提高其脂质含量。
可向微生物宿主细胞中引入基因和基因产物。用于表达基因和核酸分子的合适宿主细胞是可以在真菌或细菌家族中广泛发现的微生物宿主。合适宿主菌株的实例包括但不限于真菌或酵母物物种，例如Arxula酵母属(Arxula)、曲霉属(Aspegillus)、裂殖壶菌属(Aurantiochytrium)、假丝酵母属(Candida)、麦角菌属(Claviceps)、隐球菌属(Cryptococcus)、小克银汉霉属(Cunninghamella)、地霉属(Geotrichum)、汉逊酵母属(Hansenula)、克鲁维酵母属(Kluyveromyces)、柯达酵母属(Kodamaea)、白冬孢酵母属(Leucosporidiella)、油脂酵母属(Lipomyces)、被孢霉属(Mortierella)、Ogataea酵母属(Ogataea)、毕赤酵母属(Pichia)、原壁菌属(Prototheca)、根霉属(Rhizopus)、红冬孢酵母(Rhodosporidium)、红酵母属(Rhodotorula)、酵母属(Saccharomyces)、裂殖酵母属(Schizosaccharomyces)、银耳属(Tremella)、毛孢子菌属(Trichosporon)、威克汉姆酵母属(Wickerhamomyces)和耶氏酵母属(Yarrowia)；或者细菌物种，例如变形菌和放线菌的成员，以及以下属：不动杆菌属(Acinetobacter)、节杆菌属(Arthrobacter)、短杆菌属(Brevibacterium)、食酸菌属(Acidovorax)、芽孢杆菌属(Bacillus)、梭菌属(Clostridia)、链霉菌属(Streptomyces)、埃希氏菌属(Escherichia)、沙门氏菌属(Salmonella)、假单胞菌属(Pseudomonas)和棒杆菌属(Cornyebacterium)。解脂耶氏酵母(Yarrowia lipolytica)和Arxula酵母(Arxula adeninivorans)适合用作宿主微生物，因为它们可积累为它们重量的很大百分比的三酰甘油。
含有指导外源蛋白质的高水平表达的调控序列的微生物表达系统和表达载体是本领域技术人员已知的。可使用任何这些来构建嵌合基因以产生任一种本发明序列的基因产物。然后，可通过转化技术将这些嵌合基因引入合适的微生物中以提供酶的高水平表达。
例如，可将编码酶的基因克隆在合适的质粒中，并可用所得质粒转化上述作为宿主的起始亲本菌株。这种方法可以增加编码酶的每个基因的拷贝数，并且结果可以提高酶的活性。质粒没有特别限制，只要其使微生物后代可遗传期望的遗传修饰即可。
可用于转化合适宿主细胞的载体或盒是本领域公知的。通常来说，载体或盒包含指导相关基因的转录和翻译的序列、可选择标记和允许自主复制或染色体整合的序列。合适的载体包含携带转录起始控制的基因的5’区和控制转录终止的DNA片段的3’区。优选地，两个控制区都来源于与转化宿主细胞同源的基因时，但是应当理解，这样的控制区不需要来源于对选择作为生产宿主的的特定物种天然的基因。
启动子、cDNA、和3’UTR以及载体的其他元件可以使用分离自天然来源的片段通过克隆技术产生(Green&Sambrook,Molecular Cloning:A Laboratory Manual,(第4版,2012)；美国专利No.4,683,202；其通过引用并入)。或者，可以使用已知方法合成地生成元件(Gene 164:49-53(1995))。
B.同源重组
同源重组是互补DNA序列对齐和交换同源区的能力。将含有与所靶向基因组序列(“模板”)同源的序列的转基因DNA(“供体”)引入生物体，然后在对应同源基因组序列的位点重组到基因组中。
在宿主生物体中进行同源重组的能力对于在分子遗传学水平上可以进行的具有许多实际意义，并且可用于产生能够产生期望产物的微生物。由于其性质，同源重组是精确的基因靶向事件，并且因此用相同靶向序列产生的大多数转基因系在表型方面将基本相同，从而需要筛选远远更少的转化事件。同源重组还将基因插入事件靶向到宿主染色体中，甚至在不存在遗传选择下也可能产生优异的遗传稳定性。由于不同的染色体基因座可能会影响基因表达，甚至从外源启动子/UTR影响基因表达，因此同源重组可以是在不熟悉的基因组环境中查询基因座并评估这些环境对基因表达的影响的方法。
使用同源重组的特别有用的基因工程方法是指派特定宿主调控元件(例如启动子/UTR)从而以高度特异性方式驱动异源基因表达。
由于同源重组是精确的基因靶向事件，因此只要确定足够的侧翼区域，就可以将其用于精确修饰目的基因或区域内的任何核苷酸。因此，同源重组可用作修饰影响RNA和/或蛋白质的基因表达的调控序列的手段。其还可用于修饰蛋白质编码区域以试图修饰酶活性，例如底物特异性、亲和力和Km，从而影响宿主细胞代谢的期望变化。同源重组提供了操作宿主基因组的有力手段，产生基因靶向、基因转换、基因缺失、基因重复、基因倒位和交换基因表达调控元件(如启动子，增强子和3’UTR)。
同源重组可通过使用靶向构建体来实现，所述靶向构建体包含“靶向”内源宿主细胞基因组内的目的基因或区域的内源序列部分。这样的靶向序列可以位于目的基因或区域的5’，目的基因/区域的3’，或者甚至位于目的基因/区域的侧翼。这样的靶向构建体可以作为具有另外载体骨架的超螺旋质粒DNA、没有载体骨架的PCR产物或作为线性化分子转化到宿主细胞中。在一些情况下，首先通过用限制性酶切割转基因DNA来暴露转基因DNA(供体DNA)内的同源序列可能是有利的。该步骤可以提高重组效率并降低不期望事件的发生。提高重组效率的另一些方法包括使用PCR产生含有与所靶向基因组序列同源的线性末端的转化转基因DNA。
C.载体和载体成分
根据本发明用于转化微生物的载体可根据本文中公开内容通过本领域技术人员熟悉的已知技术来制备。载体通常包含一个或更多个基因，其中每个基因编码用于表达期望产物(基因产物)并且与一个或更多个控制序列可操作连接，所述控制序列调控基因表达或将基因产物靶向到重组细胞中的特定位置。
1.控制序列
控制序列是调控编码序列的表达或者引导基因产物到细胞内或细胞外的特定位置的核酸。调控表达的控制序列包括例如调控编码序列转录的启动子和终止编码序列转录的终止子。另一个控制序列是位于编码序列末端的编码多聚腺苷酸化信号的3’非翻译序列。引导基因产物到特定位置的控制序列包括编码信号肽的那些序列，信号肽引导与其连接的蛋白质到细胞内或细胞外的特定位置。
因此，用于在微生物中表达基因的示例性载体设计包含与在酵母中具有活性的启动子可操作连接的针对期望基因产物(例如，可选择标记或酶)的编码序列。或者，如果载体不包含与目的编码序列可操作连接的启动子，则可将编码序列转化到细胞中使得其在载体整合点变得与内源启动子可操作连接。
用于表达基因的启动子可以是与该基因天然连接的启动子或不同的启动子。
启动子通常可以被表征为组成型的或诱导型的。组成型启动子普遍具有活性并且用于随时(或在细胞生命周期中的某些时间)以相同水平驱动表达。相反，诱导型启动子仅响应于刺激而具有活性(或变得不具有活性)或者显著上调或下调。两种类型的启动子均可应用于本发明的方法。可用于本发明的诱导型启动子包括响应于刺激，例如外源提供的小分子、温度(热或冷)、培养基中缺乏氮等而介导可操作连接基因转录的那些启动子。合适的启动子可以激活基本沉默基因的转录或上调以低水平转录的可操作连接基因的转录。
任选包含终止区控制序列，并且如果使用的话，则选择主要是考虑方便性，因为终止区是相对可互换的。终止区对转录起始区(启动子)可以是天然的、对目的DNA序列可以是天然的或者可从其他来源获得(参见例如Chen和Orozco,Nucleic Acids Research 16:8411(1988))。
2.基因和密码子优化
通常来说，基因包含启动子、编码序列和终止控制序列。当通过重组DNA技术组装时，基因可被称为表达盒，并且可以以限制性位点为侧翼，所述限制性位点用于方便插入到用于将重组基因引入宿主细胞中的载体中。表达盒可以以来自基因组或其他核酸靶标的DNA序列为侧翼以有利于表达盒通过同源重组稳定整合到基因组中。或者，载体及其表达盒可以保持不整合(例如，附加体)，在这种情况下，载体通常包含能够提供载体DNA复制的复制起点。
存在于载体上的常见基因是编码这样的蛋白质的基因，其表达允许含有该蛋白质的重组细胞与不表达该蛋白质的细胞区分开。这样的基因及其对应基因产物被称为可选择标记或选择标记。在可用于转化本发明生物体的转基因构建体中可以使用任意广泛多种可选择标记。
为了最佳表达重组蛋白质，采用这样的编码序列是有益的，其产生具有待转化宿主细胞最佳使用的密码子的mRNA。因此，转基因的适当表达可要求转基因的密码子使用匹配表达转基因的生物体的特定密码子偏倚。这种效应隐藏的精确机理有很多但包括可利用氨酰基化tRNA库与细胞中正合成蛋白质的适当平衡，以及当这一需要满足时更高效地翻译转基因信使RNA(mRNA)。当转基因中的密码子使用未优化时，可用tRNA库不足以允许转基因mRNA的高校翻译，导致核糖体失速和终止以及转基因mRNA的可能的不稳定性。
C.转化
细胞可通过任何合适的技术进行转化，包括例如基因枪法、电穿孔、玻璃珠转化和碳化硅晶须转化。在本发明中可采用用于将转基因引入微生物中的任何方便技术。转化可通过例如D.M.Morrison(Methods in Enzymology 68:326(1979))的方法来实现，所述方法通过用氯化钙来增加受体细胞对DNA的渗透性(Mandel和Higa,J.Molecular Biology,53:159(1970))，等等。
在产油酵母(例如，解脂耶氏酵母)中表达转基因的实例可见于文献中(Bordes等,J.Microbiological Methods,70:493(2007)；Chen等,Applied Microbiology&Biotechnology 48:232(1997))。在细菌(例如大肠杆菌)中表达外源基因的实例是公知的(Green&Sambrook,Molecular Cloning:A Laboratory Manual,(第4版,2012)。
根据本发明用于转化微生物的载体可通过本领域技术人员熟知的已知技术来制备。在一个实施方案中，用于在微生物中表达基因的示例性载体设计包含与在微生物中具有活性的启动子可操作连接的编码酶的基因。或者，如果载体不包含与目的基因可操作连接的启动子，则可将基因转化到细胞中使得其变得在载体整合点与天然启动子可操作连接。载体还可包含编码蛋白质的第二基因。任选地，在一个或两个基因之后是包含多聚腺苷酸化信号的3’非翻译序列。编码两个基因的表达盒可在载体中物理连接或物理连接在单独的载体上。还可使用微生物的共转化，其中同时使用不同的载体分子转化细胞(Protist155:381-93(2004))。可以任选地基于在缺乏抗性盒的细胞不能生长的条件下在存在抗生素或其他选择标记下的生长的能力对细胞进行选择。
D.提高蛋白质的活性的核酸和方法
本发明的基因可以包含保守置换、缺失和/或插入，同时仍然编码具有活性的蛋白质。例如，可以针对特定宿主细胞对密码子进行优化、可为方便起见而替换不同的密码子例如以引入限制性位点或产生最佳PCR引物，或者可出于另外目的而替换密码子。类似地，可改变核苷酸序列以产生保守的氨基酸替换、缺失和/或插入。
蛋白质可包含保守替换、缺失和/或插入，同时仍维持活性。保守替换表格在本领域中是公知的(Creighton,Proteins(第2版,1992))。
氨基酸替换、缺失和/或插入可容易地使用重组DNA操作技术来进行。用于操作DNA序列以产生蛋白质的替换、插入或缺失变体的方法是本领域公知的。这些方法包括M13诱变、T7-Gen体外诱变(USB,Cleveland,OH)、快速改变定点诱变(Stratagene,San Diego,CA)、PCR介导的定点诱变以及其他定点诱变方案。
为了确定两个氨基酸序列或两个核酸序列的百分比同一性，可出于最佳比较目的对序列进行比对(例如，可以在第一和第二氨基酸或核酸序列中一个或两个中引入空位以进行最佳比对，并且可出于比较目的而忽略不相同的序列)。为了比较目的而比对的参考序列的长度可以是该参考序列的长度的至少95％。然后，可比较在对应氨基酸位置或核苷酸位置的氨基酸残基或核苷酸。当第一序列中的位置被与第二序列中对应位置的相同氨基酸残基或核苷酸占据时，则分子在该位置处是相同的(如本文中使用的，氨基酸或核酸“同一性”等同于氨基酸或核酸“同源性”)。考虑到为最佳比对两个序列而需要引入的空位的数量以及每个空位的长度，两个序列之间的百分比同一性是序列所共有相同位置的数量的函数。
序列比较和两个序列之间百分比同一性的确定可以使用数学算法来完成。在一个实施方案中，两个氨基酸序列之间的百分比同一性可使用已经并入GCG软件包(可在http://www.gcg.com获得)中GAP程序中的Needleman和Wunsch(J.Molecular Biology 48:444-453(1970))算法、使用Blosum 62矩阵或PAM250矩阵、以及16、14、12、10、8、6或4的空位权重和1、2、3、4、5或6的长度权重来确定。在另一个实施方案中，两个核苷酸序列之间的百分比同一性可使用GCG软件包(可在http://www.gcg.com获得)中的GAP程序、使用NWSgapdna.CMP矩阵以及40、50、60、70或80的空位权重和1、2、3、4、5或6的长度权重来确定。在另一个实施方案中，两个氨基酸或核苷酸序列之间的百分比同一性可使用已经并入ALIGN程序(版本2.0或2.0U)中的E.Meyers和W.Miller(Computer Applications in theBiosciences 4:11-17(1988))的算法、使用PAM120权重残基表、12的空位长度罚分和4的空位罚分来确定。
可用于确定两个序列之间同一性的示例性计算机程序包括但不限于BLAST程序套件，例如BLASTN、MEGABLAST、BLASTX、TBLASTN、TBLASTX和BLASTP；以及Clustal程序，例如ClustalW、ClustalX和Clustal Omega。
当相对于GenBank DNA序列及其他公共数据库中的核酸序列评价给定核酸序列时，通常使用BLASTN程序进行序列检索。BLASTX程序有效地用于针对GenBank蛋白质序列及其他公共数据库中的氨基酸序列检索已经在所有阅读框中翻译的核酸序列。
为了确定两个或更多个序列之间“同一性％”的所选序列的比对使用例如CLUSTAL-W程序来进行。
“编码序列”或“编码区”指具有表达序列时产生蛋白质产物(例如氨基酸或多肽)所必需的序列信息的核酸分子。编码序列可以包含在翻译区内的非翻译序列(包括内含子或着5’或3’非翻译区)和/或由其组成，或者可缺少这样的插入非翻译序列(例如，如在cDNA中)。
说明书通篇使用的涉及核酸(包含核苷酸序列和/或由核苷酸序列组成)的缩写是常规的单字母缩写。因此，当包含在核酸中时，天然存在的编码核苷酸缩写如下：腺嘌呤(A)、鸟嘌呤(G)、胞嘧啶(C)、胸腺嘧啶(T)和尿嘧啶(U)。此外，除非另外说明，否则本文提供的核酸序列为5'→3'方向。
本文中使用的术语“互补的”及其衍生词用于指通过以下公知规则的核酸配对：A与T或U配对，C与G配对。互补可以是“部分的”或“完全的”。在部分互补中，只有一些核酸碱基根据碱基配对规则进行匹配；在完全或全部互补中，所有的碱基都根据配对规则进行匹配。如本领域中公知的，核酸链之间的互补程度可对核酸链之间杂交的效率和强度具有显著影响。所述杂交的效率和强度取决于检测方法。
氨基酸和核苷酸序列可来源于具有高天然水平的脂质积累的产油生物。(Bioresource Technology 144:360-69(2013)；Progress Lipid Research52:395-408(2013)；Applied Microbiology&Biotechnology 90:1219-27(2011)；European JournalLipid Science&Technology 113:1031-51(2011)；Food Technology&Biotechnology 47:215-20(2009)；Advances Applied Microbiology 51:1-51(2002)；Lipids 11:837-44(1976))。报道脂质含量为约50％及更高的生物体的列表示于表1中。圆红冬孢酵母属(R.toruloides)和斯达油脂酵母属(L.starkeyi)具有最高的脂质含量。
表1
报道脂质含量为约50％及以上的产油真菌的列表
通过过表达蛋白质可提高蛋白质的活性。可使用多种遗传修饰来在细胞中过表达蛋白质。在一些实施方案中，遗传修饰提高天然蛋白质的表达。可通过修饰编码蛋白质的基因的上游转录调节子，例如通过提高转录激活物的表达或降低转录阻遏物的表达来过表达天然蛋白质。或者，可以通过与外源核酸重组用组成活性或诱导型启动子取代天然基因的启动子。
在一些实施方案中，提高蛋白质活性的遗传修饰包括用包含编码该蛋白质的基因的核酸进行转化。所述基因可以是细胞天然的或来自不同的物种。在某些实施方案中，所述基因对转化细胞的后代是可遗传的。在一些实施方案中，所述基因由于其存在于质粒上而是可遗传的。在某些实施方案中，所述基因由于其整合到转化细胞的基因组中而是可遗传的。
E.降低天然蛋白质的活性的核酸和方法
在一些实施方案中，转化的产油细胞包含降低天然蛋白质活性的遗传修饰。这种遗传修饰可影响调控天然蛋白质转录的蛋白质，包括降低转录激活物表达和/或提高转录阻遏物表达的修饰。影响调节蛋白质的修饰可降低天然蛋白质的表达以及改变使细胞平衡向增加油酸积聚移动的其他基因表达谱。或者，遗传修饰可为引入干扰核酸，例如小干扰RNA或编码干扰核酸的核酸。在另一些实施方案中，遗传修饰由编码天然蛋白质的基因的核酸和调控区的同源重组组成。基因的调控区可包括操纵子、启动子、启动子上游的序列、增强子和/或基因下游的序列。
在一些实施方案中，转化的产油细胞包含由同源重组事件组成的遗传修饰。在某些实施方案中，转化细胞包含由天然基因和核酸之间的同源重组事件组成的遗传修饰。因此，遗传修饰缺失天然基因、阻止其转录、或者阻止可翻译成完全活性蛋白质的基因转录。同源重组事件可以突变或缺失天然基因的一部分。例如，同源重组事件可突变天然酶的活性位点中的一个或更多个残基，从而降低酶的效率或使其无活性。或者，同源重组事件可影响翻译后修饰、折叠、稳定性或在细胞内的定位。在一些实施方案中，同源重组事件用驱动较低转录的启动子取代启动子。在另一些实施方案中，同源重组事件突变启动子以损害其驱动转录的能力。在某些实施方案中，遗传修饰是敲除突变。
敲除突变可缺失一个或更多个基因。另外，敲除突变可用编码不同蛋白质的外源基因替代天然基因。外源基因可与外源启动子可操作连接。在某些实施方案中，该基因不与外源启动子连接，而是将该基因配置为与天然基因重组使得天然基因的启动子驱动外源基因的转录。因此，如果基因随机整合到细胞的基因组中，则该基因不太可能被表达。用于产生敲除的方法在本领域中是公知的(参见例如Fickers等,J.Microbiological Methods55:727(2003))。
在某些实施方案中，遗传修饰包括两个同源重组事件。在第一事件中，编码基因一部分的核酸与天然基因重组，在第二事件中，编码基因剩余部分的核酸与天然基因重组。基因的这两个部分被设计成使得除非其彼此重组否则任一部分均不具有功能性。这两个事件进一步降低基因在随机整合事件后表达的可能性。
在某些实施方案中，基因编码标记蛋白质，例如显性可选择标记。因此，可通过筛选标记来选择敲除细胞。在一些实施方案中，显性可选择标记是抗药性标记。抗药性标记是这样的显性选择标记，其当由细胞表达时允许细胞在通常抑制细胞生长和/或存活的药物存在下生长和/或存活。可以通过在药物存在下培养细胞来选择表达抗药性标记的细胞。在一些实施方案中，抗药性标记是抗生素抗性标记。在一些实施方案中、抗药性标记赋予对选自以下的药物的抗性：两性霉素B、克念菌素、非律平、哈霉素、那他霉素、制霉菌素、龟裂霉素、联苯苄唑、布康唑、克霉唑、益康唑、芬替康唑、异康唑、酮康唑、卢立康唑、咪康唑、奥莫康唑、奥昔康唑、舍他康唑、硫康唑、噻康唑、阿巴康唑、氟康唑、艾沙康唑、伊曲康唑、泊沙康唑、雷夫康唑、特康唑、伏立康唑、阿巴芬净、阿莫洛芬(Amorolfin)、布替萘芬、萘替芳、特比萘芬、阿尼芬净、卡泊芬净、米卡凡金、苯甲酸、环吡酮、氟胞嘧啶、5-氟胞嘧啶、灰黄霉素、卤普罗近、水蓼二醛、托萘酯、结晶紫、阿米卡星、庆大霉素、卡那霉素、新霉素、奈替米星、托普霉素、巴龙霉素、大观霉素、格尔德霉素、除莠霉素、利福昔明、链霉素、氯碳头孢、厄他培南、多尼培南、亚胺培南、美罗培南、头孢羟氨苄、头孢唑林、头孢噻吩、头孢氨苄、头孢克洛、头孢孟多、头孢西丁、头孢丙烯、头孢呋辛、头孢克肟、头孢地尼、头孢托仑、头孢哌酮、头孢噻肟酸、头孢泊肟、头孢他啶、头孢布烯、头孢唑肟、头孢曲松、头孢吡肟、头孢洛林酯(Ceftaroline fosamil)、头孢比普、替考拉宁、万古霉素、泰拉万星、克林霉素、林肯霉素、达托霉素、阿齐霉素、克拉霉素、地红霉素、红霉素、罗红霉素、醋竹桃霉素、泰利霉素、螺旋霉素、氨曲南、呋喃唑酮、呋喃妥因、利奈唑胺、泊西唑利德(Posizolid)、雷得唑来(Radezolid)、泰地唑胺(Torezolid)、阿莫西林、氨苄西林、阿洛西林、羧苄西林、氯唑西林、双氯西林、氟氯西林、美洛西林、甲氧西林、萘夫西林、苯唑西林、青霉素G、青霉素V、哌拉西林、青霉素G、替莫西林、替卡西林、克拉维、舒巴坦、他佐巴坦、克拉维、杆菌肽、黏菌素、多黏菌素B、环丙沙星、依诺沙星、加替沙星、左氧氟沙星、洛美沙星、莫西沙星、萘啶酸、诺氟沙星、氧氟沙星、曲伐沙星、格帕沙星、司帕沙星、替马沙星、磺胺米隆、磺胺醋酰、磺胺嘧啶、磺胺嘧啶银、磺胺地索辛、磺胺甲二唑、磺胺甲唑、对氨基苯磺酰胺(sulfanilimide)、硫氮磺吡啶、硫代异唑、甲氧苄啶-磺胺甲基异唑、复方新诺明、磺胺柯衣定(Sulfonamidochrysoidine)、地美环素、多西环素、米诺环素、氧四环素、四环素、氯苯吩嗪、氨苯砜、卷曲霉素、环丝氨酸、乙胺丁醇、乙硫异烟胺、异烟肼、吡嗪酰胺、利福平、利福布汀、利福喷汀、链霉素、胂凡纳明、氯霉素、磷霉素、梭链孢酸、甲硝唑、莫匹罗星、平板霉素、奎奴普丁、达福普汀、甲砜霉素、替加环素、替硝唑、甲氧苄啶、遗传霉素、诺尔丝菌素、潮霉素、博来霉素和嘌呤霉素。
在一些实施方案中，显性可选择标记是营养标记。营养标记是这样的显性可选择标记，当由细胞表达时使细胞能够使用一种或更多种特定营养源生长或存活。表达营养标记的细胞可通过在限制营养条件下培养细胞来选择，其中表达营养标记的细胞可存活和/或生长，但是缺乏营养标记的细胞则不能。在一些实施方案中，营养标记选自乳清苷5-磷酸脱羧酶、亚磷酸盐特异性氧化还原酶、α-酮戊二酸盐依赖性次磷酸盐加双氧酶、碱性磷酸酶、氰酰胺水合酶、三聚氰胺脱氨酶、氰脲酸盐氨基水解酶、缩二脲水解酶、脲酰胺裂解酶、三聚氰酸一酰胺氨基水解酶、鸟嘌呤脱氨酶、磷酸二酯酶、磷酸三酯酶、亚磷酸盐氢化酶、甘油磷酸二酯酶、对硫磷水解酶、亚磷酸盐脱氢酶、二苯并噻吩脱硫酶、芳族脱亚磺酸酶、FMN还原酶、NADH依赖性FMN还原酶、氨基嘌呤转运蛋白、羟胺氧化还原酶、转化酶、β-葡糖苷酶、α-葡糖苷酶、β-半乳糖苷酶、α-半乳糖苷酶、淀粉酶、纤维素酶和普鲁兰酶。
可使用不同的方法来敲除酵母细胞中的基因(参见例如Dulermo等,BiochimicaBiophysica Acta 1831:1486(2013))。本文公开的方法以及本领域已知的其他方法可用于敲除其他物种(例如Arxula酵母)中的不同基因。
在一些实施方案中，遗传修饰使天然基因的表达降低0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、1％、2％、3％、4％、5％、6％、7％、8％、9％、10％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或100％。
在一些实施方案中，遗传修饰使天然蛋白质的效率降低0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、1％、2％、3％、4％、5％、6％、7％、8％、9％、10％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或100％。
在一些实施方案中，遗传修饰使天然蛋白质的活性降低0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、1％、2％、3％、4％、5％、6％、7％、8％、9％、10％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或100％。
示例性细胞、核酸和方法
A.转化细胞
在一些实施方案中，转化细胞是原核细胞，例如细菌细胞。在一些实施方案中，细胞是真核细胞，例如哺乳动物细胞、酵母细胞、丝状真菌细胞、原生生物细胞、藻类细胞、禽细胞、植物细胞或昆虫细胞。在一些实施方案中，细胞是酵母。本领域技术人员将认识到许多形式的丝状真菌产生酵母样生长，并且本文中酵母的定义包括这样的细胞。
细胞可以选自Arxula酵母属、曲霉属、裂殖壶菌属、假丝酵母属、麦角菌属、隐球菌属、小克银汉霉属、地霉属、汉逊酵母属、克鲁维酵母属、柯达酵母属、白冬孢酵母属、油脂酵母属、被孢霉属、Ogataea酵母属、毕赤酵母属、原壁菌属、根霉属、红冬孢酵母属、红酵母属、酵母属、裂殖酵母属、银耳属、毛孢子菌属、威克汉姆酵母属和耶氏酵母属。
在一些实施方案中，细胞选自Arxula酵母、黑曲霉、米曲霉、土曲霉、裂殖壶菌、产朊假丝酵母、麦角菌、白色隐球菌、弯曲隐球菌、新型隐球菌、地生隐球酵母、威氏隐球菌、刺孢小克银汉霉、山茶小克银汉霉、发酵地霉、多形汉逊酵母、乳酸克鲁维酵母、马克思克鲁维酵母、奥默柯达菌、冰岛白冬孢酵母、产油油脂酵母、斯达油脂酵母、子囊菌油脂酵母、深黄被孢霉、高山被孢霉、Ogataea酵母、西弗毕赤酵母、季也蒙毕赤酵母、巴斯德毕赤酵母、树干毕赤酵母、左氏原壁菌、无根根霉、巴布维红冬孢酵母、圆红冬孢酵母、沼泽生红冬孢酵母、黏红酵母、胶红酵母、酿酒酵母、粟酒裂殖酵母、银耳、皮肤毛孢子菌、发酵毛孢子菌、威克汉姆西弗酵母和解脂耶氏酵母。
在某些实施方案中，细胞是酿酒酵母、解脂耶氏酵解酵母或Arxula酵母。在某些实施方案中，细胞不是酿酒酵母。
在一些实施方案中，细胞是酵母、真菌或酵母样藻类。细胞可选自破囊壶菌(裂殖壶菌属)和无叶绿素单细胞藻类(原壁菌属)。
在某些实施方案中，如通过干细胞重量％测量的，转化细胞包含至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％或更多脂质。
B.包含磷酸转酮酶和磷酸乙酰转移酶活性的细胞
包含磷酸转酮酶和磷酸乙酰转移酶活性的细胞可以催化木酮糖-5-磷酸转化为乙酰CoA，这允许细胞在细细胞溶质中由葡萄糖产生乙酰CoA，从而相对于依赖线粒体途径的细胞增加细胞溶质NADPH(图2和图3)。
存在三种形式的磷酸转酮酶：作用于五碳木酮糖-5-磷酸(X-5-P)的那些EC4.1.2.9；作用于六碳果糖-6-磷酸(F-6-P)的那些，EC 4.1.2.22；以及作用于两种底物的具有双功能活性的那些。6-碳磷酸转酮酶与存在于所有微生物中的转酮醇酶(EC 2.2.1.1)一起催化与5-碳磷酸转酮酶相同的木酮糖-5-磷酸向乙酰磷酸(Ac-P)和甘油醛-3-磷酸(Ga-3-P)净转化的反应。转酮醇酶将木酮糖-5-磷酸(X-5-P)和赤藓糖-4-磷酸(E-4-P)转化成果糖-6-磷酸(F-6-P)和甘油醛-3-磷酸(Ga-3-P)。
i)EC 4.1.2.9X-5-P+Pi->Ac-P+Ga-3-P+H2O
ii)EC 4.1.2.22 F-6-P+Pi->Ac-P+E-4-P+H2O
EC 2.2.1.1 X-5-P+E-4-P->F-6-P+Ga-3-P
净:X-5-P+Pi->Ac-P+Ga-3-P+H2O
将编码磷酸转酮酶和磷酸乙酰转移酶蛋白质的基因引入细胞中的方法是本领域已知的(参见例如美国专利申请公开No.2012/0156735，其通过引用并入本文)。
在一些实施方案中，本发明涉及一种转化细胞，其包含第一遗传修饰和第二遗传修饰，其中第一遗传修饰提高细胞中磷酸转酮酶蛋白质的活性，并且第二遗传修饰提高细胞中磷酸乙酰转移酶蛋白质的活性。在一些实施方案中，转化细胞可催化上述磷酸转酮酶/磷酸乙酰转移酶途径。在一些实施方案中，转化细胞不催化磷酸转酮酶/磷酸乙酰转移酶途径，例如细胞可利用磷酸转酮酶和/或磷酸乙酰转移酶蛋白质将木酮糖-5-磷酸、果糖-6-磷酸或不同底物(例如不同糖)转化成乙酰磷酸和甘油醛-3-磷酸酯或者不同的产品；或者例如细胞可用作用于分子克隆的宿主细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码磷酸转酮酶蛋白质的第一核苷酸序列转化所述细胞，以及用编码磷酸乙酰转移酶蛋白质的第二核苷酸序列转化所述细胞。所述方法可通过完成上述磷酸转酮酶/磷酸乙酰转移酶途径来提高细胞的脂质含量。在一些实施方案中，所述方法通过除磷酸转酮酶/磷酸乙酰转移酶途径之外的分子途径来提高细胞的脂质含量，例如转化细胞可利用磷酸转酮酶和/或磷酸乙酰转移酶蛋白质将木酮糖-5-磷酸、果糖-6-磷酸或不同底物(例如不同糖)转化成乙酰磷酸和甘油醛-3-磷酸或者不同的产物。磷酸转酮酶和/或磷酸乙酰转移酶可催化不同代谢途径，例如趋于提高脂质生物合成或降低脂质降解的代谢途径中的步骤。
在一些实施方案中，磷酸转酮酶蛋白质通过酶学委员会编号EC 4.1.2.9或EC4.1.2.22分类。
磷酸转酮酶蛋白质可包含与SEQ ID NO:21、SEQ ID NO:23、SEQ ID NO:25、SEQ IDNO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ ID NO:125所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，磷酸转酮酶蛋白质可与SEQ ID NO:21、SEQ ID NO:23、SEQ ID NO:25、SEQ IDNO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ ID NO:125基本上相同，并且保留SEQ ID NO:21、SEQ ID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ IDNO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ ID NO:125的蛋白质的功能活性，但是氨基酸序列不同，例如由于天然等位基因变异或诱变而不同。在一些实施方案中，磷酸转酮酶蛋白质包含SEQ ID NO:21、SEQ ID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ ID NO:125的氨基酸序列。
第一遗传修饰可以是第一核酸的转化，并且第一核酸可编码磷酸转酮酶蛋白质，即第一核酸可包含编码磷酸转酮酶蛋白质的第一核苷酸序列。
在一些实施方案中，第一核苷酸序列与SEQ ID NO:22、SEQ ID NO:24、SEQ ID NO:26、SEQ ID NO:28、SEQ ID NO:30、SEQ ID NO:32、SEQ ID NO:122、SEQ ID NO:124或SEQ IDNO:126所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性。例如，第一核苷酸序列可具有SEQ ID NO:22、SEQ IDNO:24、SEQ ID NO:26、SEQ ID NO:28、SEQ ID NO:30、SEQ ID NO:32、SEQ ID NO:122、SEQID NO:124或SEQ ID NO:126所示的序列。在一些实施方案中，第一核苷酸序列与SEQ IDNO:22、SEQ ID NO:24、SEQ ID NO:26、SEQ ID NO:28、SEQ ID NO:30、SEQ ID NO:32、SEQ IDNO:122、SEQ ID NO:124或SEQ ID NO:126所示的序列基本上相同，并且第一核苷酸序列编码保留由SEQ ID NO:21、SEQ ID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ ID NO:29、SEQID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ ID NO:125编码的蛋白质的活性的磷酸转酮酶蛋白质，但是第一核苷酸序列与SEQ ID NO:22、SEQ ID NO:24、SEQ ID NO:26、SEQ IDNO:28、SEQ ID NO:30、SEQ ID NO:32、SEQ ID NO:122、SEQ ID NO:124或SEQ ID NO:126所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，第一核苷酸序列编码SEQ ID NO:21、SEQ ID NO:23、SEQ IDNO:25、SEQ ID NO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQID NO:125所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，第一核苷酸序列可编码SEQ ID NO:21、SEQ ID NO:23、SEQ IDNO:25、SEQ ID NO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQID NO:125所示的氨基酸序列。在一些实施方案中，第一核苷酸序列编码与SEQ ID NO:21、SEQ ID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ ID NO:125所示的序列基本上相同的序列，并且第一核苷酸序列编码保留由SEQ ID NO:21、SEQ ID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ ID NO:125编码的蛋白质的活性的磷酸转酮酶蛋白质，但是第一核苷酸序列编码与SEQ ID NO:21、SEQ ID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ IDNO:125所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，磷酸乙酰转移酶蛋白质通过酶学委员会编号EC2.3.1.8分类。
磷酸乙酰转移酶蛋白质可包含与SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ IDNO:115、SEQ ID NO:117、SEQ ID NO:119所示的序列或其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，磷酸乙酰转移酶蛋白质可与SEQ IDNO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ IDNO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ IDNO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ IDNO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ IDNO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ IDNO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119基本上相同，并且保留SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ IDNO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ IDNO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ IDNO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119的蛋白质的功能活性，但是氨基酸序列不同，例如由于于天然等位基因变异或诱变而不同。在一些实施方案中，磷酸乙酰转移酶蛋白质包含SEQ ID NO:1、SEQID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ IDNO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ IDNO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ IDNO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ IDNO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ IDNO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ IDNO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119所示的氨基酸序列。
第二遗传修饰可以是第二核酸的转化，并且第二核酸可编码磷酸乙酰转移酶蛋白质，即第二核酸可包含编码磷酸乙酰转移酶蛋白质的第二核苷酸序列。
在一些实施方案中，第二核苷酸序列与SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:34、SEQ ID NO:36、SEQ ID NO:38、SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:44、SEQ ID NO:46、SEQ ID NO:48、SEQ ID NO:50、SEQ ID NO:52、SEQ ID NO:54、SEQ IDNO:56、SEQ ID NO:58、SEQ ID NO:60、SEQ ID NO:62、SEQ ID NO:64、SEQ ID NO:66、SEQ IDNO:68、SEQ ID NO:70、SEQ ID NO:72、SEQ ID NO:74、SEQ ID NO:76、SEQ ID NO:78、SEQ IDNO:80、SEQ ID NO:82、SEQ ID NO:84、SEQ ID NO:86、SEQ ID NO:88、SEQ ID NO:90、SEQ IDNO:92、SEQ ID NO:94、SEQ ID NO:96、SEQ ID NO:98、SEQ ID NO:100、SEQ ID NO:102、SEQID NO:104、SEQ ID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ ID NO:118、or SEQ ID NO:120所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性。例如，第二核苷酸序列可具有SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:34、SEQ IDNO:36、SEQ ID NO:38、SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:44、SEQ ID NO:46、SEQ IDNO:48、SEQ ID NO:50、SEQ ID NO:52、SEQ ID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ IDNO:60、SEQ ID NO:62、SEQ ID NO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ IDNO:72、SEQ ID NO:74、SEQ ID NO:76、SEQ ID NO:78、SEQ ID NO:80、SEQ ID NO:82、SEQ IDNO:84、SEQ ID NO:86、SEQ ID NO:88、SEQ ID NO:90、SEQ ID NO:92、SEQ ID NO:94、SEQ IDNO:96、SEQ ID NO:98、SEQ ID NO:100、SEQ ID NO:102、SEQ ID NO:104、SEQ ID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ IDNO:118或SEQ ID NO:120所示的序列。在一些实施方案中，第二核苷酸序列与SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:34、SEQ ID NO:36、SEQ ID NO:38、SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:44、SEQ ID NO:46、SEQ ID NO:48、SEQ ID NO:50、SEQ ID NO:52、SEQ ID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ ID NO:60、SEQ ID NO:62、SEQ IDNO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ ID NO:72、SEQ ID NO:74、SEQ IDNO:76、SEQ ID NO:78、SEQ ID NO:80、SEQ ID NO:82、SEQ ID NO:84、SEQ ID NO:86、SEQ IDNO:88、SEQ ID NO:90、SEQ ID NO:92、SEQ ID NO:94、SEQ ID NO:96、SEQ ID NO:98、SEQ IDNO:100、SEQ ID NO:102、SEQ ID NO:104、SEQ ID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ ID NO:118或SEQ ID NO:120所示的序列基本上相同，并且第二核苷酸序列编码保留由SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ IDNO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ IDNO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ IDNO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ IDNO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119编码蛋白质的活性的磷酸乙酰转移酶蛋白质，但是第二核苷酸序列与SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:34、SEQ ID NO:36、SEQ ID NO:38、SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:44、SEQ IDNO:46、SEQ ID NO:48、SEQ ID NO:50、SEQ ID NO:52、SEQ ID NO:54、SEQ ID NO:56、SEQ IDNO:58、SEQ ID NO:60、SEQ ID NO:62、SEQ ID NO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ IDNO:70、SEQ ID NO:72、SEQ ID NO:74、SEQ ID NO:76、SEQ ID NO:78、SEQ ID NO:80、SEQ IDNO:82、SEQ ID NO:84、SEQ ID NO:86、SEQ ID NO:88、SEQ ID NO:90、SEQ ID NO:92、SEQ IDNO:94、SEQ ID NO:96、SEQ ID NO:98、SEQ ID NO:100、SEQ ID NO:102、SEQ ID NO:104、SEQID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ ID NO:118或SEQ ID NO:120所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，第二核苷酸序列编码与SEQ ID NO:1、SEQ ID NO:3、SEQ IDNO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ IDNO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ IDNO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ IDNO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ IDNO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ IDNO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，第二核苷酸序列可编码SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ IDNO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ IDNO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ IDNO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ IDNO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ IDNO:119所示的氨基酸序列。在一些实施方案中，第二核苷酸序列编码SEQ ID NO:1、SEQ IDNO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ IDNO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ IDNO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ IDNO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ IDNO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ IDNO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ IDNO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119基本上相同的氨基酸序列，并且第二核苷酸序列编码保留由SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ IDNO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ IDNO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ IDNO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ IDNO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119编码的蛋白质的活性的磷酸乙酰转移酶蛋白质，但是第二核苷酸序列编码与SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ IDNO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ IDNO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ IDNO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ IDNO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ IDNO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ IDNO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，转化细胞不包含天然丙酮酸脱羧酶基因中的缺失、突变或置换。在一些实施方案中，转化细胞不包含编码将丙酮酸转化成乙醛、乙酰磷酸或乙酰CoA的多肽的天然基因中的缺失、突变或置换。在一些实施方案中，转化细胞包含丙酮酸脱羧酶蛋白质。
1.降低磷酸果糖激酶蛋白质、果糖二磷酸醛缩酶蛋白质和/或丙糖磷酸异构酶蛋
白质的活性
降低天然磷酸果糖激酶蛋白质、天然果糖二磷酸醛缩酶蛋白质或天然丙糖磷酸异构酶蛋白质的活性阻碍与磷酸转酮酶途径竞争的细胞途径，从而提高磷酸转酮酶途径的利用(图2)。
a.降低磷酸果糖激酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然磷酸果糖激酶蛋白质的活性的遗传修饰，例如细胞可包含天然磷酸果糖激酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然磷酸果糖激酶酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然磷酸果糖激酶基因中的核苷酸序列和/或天然磷酸果糖激酶基因的调控区中的核苷酸序列重组。
磷酸果糖激酶蛋白质可通过酶学委员会编号EC 2.7.1.11分类。
b.降低果糖二磷酸醛缩酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然果糖二磷酸醛缩酶蛋白质的活性的遗传修饰，例如细胞可包含天然果糖-二磷酸醛缩酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然果糖二磷酸醛缩酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然果糖二磷酸醛缩酶基因中的核苷酸序列和/或天然果糖二磷酸醛缩酶基因的调控区中的核苷酸序列重组。
果糖二磷酸醛缩酶蛋白质可通过酶学委员会编号EC 4.1.2.13分类。
c.降低丙糖磷酸异构酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然丙糖磷酸异构酶蛋白质的活性的遗传修饰，例如细胞可包含天然丙糖磷酸异构酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然丙糖磷酸异构酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然丙糖磷酸异构酶基因中的核苷酸序列和/或天然丙糖磷酸异构酶基因的调控区中的核苷酸序列重组。
丙糖磷酸异构酶蛋白质可通过酶学委员会编号EC 5.3.1.1分类。
2.提高果糖-1,6-二磷酸酶蛋白质的活性并降低磷酸果糖激酶蛋白质的活性
提高细胞中果糖-1,6-二磷酸酶蛋白质的活性提高了甘油醛-3-磷酸向葡萄糖-6-磷酸的转化，这为磷酸转酮酶反应的甘油醛-3-磷酸产物提供吸收物，并增加葡萄糖-6-磷酸前体的浓度，从而提高磷酸转酮酶途径的利用(图3)。降低天然磷酸果糖激酶蛋白质的活性阻碍与果糖-1,6-二磷酸酶途径竞争的细胞途径，从而提高果糖-1,6-二磷酸酶途径的利用。
a.提高果糖-1,6-二磷酸酶蛋白质的活性
在一些实施方案中，转化细胞包含第三遗传修饰，其中所述第三遗传修饰提高细胞中果糖-1,6-二磷酸酶蛋白质的活性。第三遗传修饰可以是用第三核酸的转化，并且第三核酸可编码果糖-1,6-二磷酸酶蛋白质。
在一些实施方案中，所述方法包括用第三核苷酸序列转化细胞，其中所述第三核苷酸序列提高细胞中果糖-1,6-二磷酸酶蛋白质的活性。例如，第三核苷酸序列可编码果糖-1,6-二磷酸酶蛋白质。
果糖-1,6-二磷酸酶蛋白质可通过酶学委员会编号EC 3.1.3.11分类。
b.降低磷酸果糖激酶蛋白质的活性
在一些实施方案中，转化细胞包含降低细胞中天然磷酸果糖激酶蛋白质的活性的第四遗传修饰，例如细胞可包含天然磷酸果糖激酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用第四核苷酸序列转化细胞，其中所述第四核苷酸序列降低细胞中天然磷酸果糖激酶蛋白质的活性。例如，第四核苷酸序列可以能够与天然磷酸果糖激酶基因中的核苷酸序列和/或天然磷酸果糖激酶基因的调控区中的核苷酸序列重组。
磷酸果糖激酶蛋白质可通过学酶委员会编号EC 2.7.1.11分类。
c.降低转醛醇酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然转醛醇酶蛋白质的活性的遗传修饰，例如细胞可包含天然转醛醇酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然转醛醇酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然转醛醇酶基因中的核苷酸序列和/或天然转醛醇酶基因的调控区中的核苷酸序列重组。
转醛醇酶蛋白质可通过酶学委员会号EC 2.2.1.2分类。
d.降低甘油醛3-磷酸脱氢酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然甘油醛3-磷酸脱氢酶蛋白质的活性的遗传修饰，例如细胞可包含天然甘油醛3-磷酸脱氢酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然甘油醛3-磷酸脱氢酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然甘油醛-3-磷酸脱氢酶基因中的核苷酸序列和/或天然甘油醛-3-磷酸脱氢酶基因的调控区中的核苷酸序列重组。
甘油醛-3-磷酸脱氢酶蛋白质可通过酶学委员会编号EC 1.2.1.12分类。
e.降低磷酸甘油酸激酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然磷酸甘油酸激酶蛋白质的活性的遗传修饰，例如细胞可包含天然磷酸甘油酸激酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然磷酸甘油酸激酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然磷酸甘油酸激酶基因的核苷酸序列中和/或天然磷酸甘油酸激酶基因的调控区中的核苷酸序列重组。
磷酸甘油酸激酶蛋白质可通过酶学委员会编号EC 2.7.2.3分类。
f.降低磷酸甘油酸变位酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然磷酸甘油酸变位酶蛋白质的活性的遗传修饰，例如细胞可包含天然磷酸甘油酸变位酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然磷酸甘油酸变位酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然磷酸甘油酸变位酶基因中的核苷酸序列和/或天然磷酸甘油酸变位酶基因的调控区中的核苷酸序列重组。
磷酸甘油酸变位酶蛋白质可通过酶学委员会编号EC 5.4.2.11分类。
g.降低烯醇化酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然烯醇化酶蛋白质的活性的遗传修饰，例如细胞可包含天然烯醇化酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然烯醇化酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然烯醇化酶基因中的核苷酸序列和/或天然烯醇化酶基因的调控区中的核苷酸序列重组。
烯醇化酶蛋白质可通过酶学委员会编号EC 4.2.1.11分类。
h.降低丙酮酸激酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然丙酮酸激酶蛋白质的活性的遗传修饰，例如细胞可包含天然丙酮酸激酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然丙酮酸激酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然丙酮酸激酶基因中的核苷酸序列和/或天然丙酮酸激酶基因的调控区中的核苷酸序列重组。
丙酮酸激酶蛋白质可通过酶学委员会编号EC 2.7.1.40分类。
i.提高氧化还原酶蛋白质的活性
在一些实施方案中，转化细胞还包含提高细胞中氧化还原酶蛋白质的活性的遗传修饰。例如，遗传修饰可以是编码氧化还原酶蛋白质的核酸的转化。
在一些实施方案中，所述方法还包括用提高细胞中氧化还原酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可编码氧化还原酶蛋白质。
在一些实施方案中，转化细胞还包含提高细胞中NADPH外部氧化还原酶蛋白质的活性的遗传修饰。例如，所述遗传修饰可以是编码NADPH外部氧化还原酶蛋白质的核酸的转化。
在一些实施方案中，所述方法还包括用提高细胞中NADPH外部氧化还原酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可编码NADPH外部氧化还原酶蛋白质。
j.提高可溶性转氢酶蛋白质的活性
在一些实施方案中，转化细胞还包含提高细胞中转氢酶蛋白质的活性的遗传修饰。例如，所述遗传修饰可以是使用编码转氢酶蛋白质的核酸的转化。
在一些实施方案中，所述方法还包括使用提高细胞中转氢酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可编码转氢酶蛋白质。
在一些实施方案中，转化细胞还包含提高细胞中可溶性NADPH:NADH转氢酶蛋白质的活性的遗传修饰。例如，所述遗传修饰可以是编码可溶性NADPH:NADH转氢酶蛋白质的核酸的转化。
在一些实施方案中，所述方法还包括用提高细胞中可溶性NADPH:NADH转氢酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可编码可溶性NADPH:NADH转氢酶蛋白质。
C.包含丙酮酸脱羧酶活性、磷酸乙酰转移酶活性和乙酸激酶活性的细胞
提高细胞中丙酮酸脱羧酶、磷酸乙酰转移酶和乙酸激酶的活性允许细胞由细胞溶质中的丙酮酸产生乙酰CoA(图4)。
在一些实施方案中，本发明涉及一种转化细胞，其包含第一遗传修饰、第二遗传修饰和第三遗传修饰，其中所述第一遗传修饰提高细胞中丙酮酸脱羧酶蛋白质的活性，所述第二遗传修饰提高细胞中磷酸乙酰转移酶蛋白质的活性，并且所述第三遗传修饰提高细胞中乙酸激酶的活性。在一些实施方案中，转化细胞由细胞溶质中的丙酮酸生成乙酰CoA。在一些实施方案中，转化细胞不由细胞溶质中的丙酮酸产生乙酰CoA，例如细胞可将丙酮酸或不同底物转化成乙酰CoA或不同产物；或者例如细胞可用作用于分子克隆的宿主细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码丙酮酸脱羧酶蛋白质的第一核苷酸序列转化所述细胞，用编码磷酸乙酰转移酶蛋白质的第二核苷酸序列转化所述细胞，以及用编码乙酸激酶蛋白质的第三核苷酸序列转化所述细胞。在一些实施方案中，所述方法通过由丙酮酸至乙酰CoA的细胞溶质生产来提高细胞的脂质含量。在一些实施方案中，所述方法通过除由丙酮酸至乙酰CoA的细胞溶质生产之外的分子途径来提高细胞的脂质含量，例如转化细胞可利用丙酮酸脱羧酶、磷酸乙酰转移酶和/或乙酸激酶蛋白质将丙酮酸或不同底物转化成乙酰CoA或不同产物。例如，丙酮酸脱羧酶、磷酸乙酰转移酶和/或乙酸激酶可催化提高脂质生物合成或降低脂质降解的不同代谢途径中的步骤。
a.提高丙酮酸脱羧酶蛋白质的活性
在一些实施方案中，丙酮酸脱羧酶蛋白质通过酶学委员会编号EC 4.1.1.1分类。
丙酮酸脱羧酶蛋白质可包含与SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，丙酮酸脱羧酶蛋白质可与SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11基本上相同并且保留SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11的蛋白质的功能活性，但是氨基酸序列不同，例如由于天然等位基因变异或诱变而不同。在一些实施方案中，丙酮酸脱羧酶蛋白质包含SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11所示的氨基酸序列。
第一遗传修饰可以是使用第一核酸的转化，并且第一核酸可编码丙酮酸脱羧酶蛋白质，即第一核酸可包含编码丙酮酸脱羧酶蛋白质的第一核苷酸序列。
在一些实施方案中，第一核苷酸序列与SEQ ID NO:8、SEQ ID NO:10或SEQ ID NO:12所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性。例如，第一核苷酸序列可具有SEQ ID NO:8、SEQ ID NO:10或SEQID NO:12所示的序列。在一些实施方案中，第一核苷酸序列与SEQ ID NO:8、SEQ ID NO:10或SEQ ID NO:12所示的序列基本上相同，并且第一核苷酸序列编码保留由SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11编码的蛋白质的活性的丙酮酸脱酸酶蛋白质，但是第一核苷酸序列与SEQ ID NO:8、SEQ ID NO:10或SEQ ID NO:12所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，第一核苷酸序列编码与SEQ ID NO:7、SEQ ID NO:9或SEQ IDNO:11所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，第一核苷酸序列可编码SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11所示的氨基酸序列。在一些实施方案中，第一核苷酸序列编码与SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11所示的序列基本上相同的氨基酸序列，并且第一核苷酸序列编码保留由SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11编码的蛋白质的活性的丙酮酸脱羧酶，但是第一核苷酸序列编码与SEQ ID NO:7、SEQ ID NO:9或SEQ ID NO:11所示的序列不同，例如由于天然等位基因变异或诱变而不同。
b.提高磷酸乙酰转移酶蛋白质的活性
在一些实施方案中，磷酸乙酰转移酶蛋白质通过酶学委员会号EC 2.2.1.8分类。
磷酸乙酰转移酶蛋白质可包含与SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ IDNO:115、SEQ ID NO:117或SEQ ID NO:119所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，磷酸乙酰转移酶蛋白质可与SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ IDNO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ IDNO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ IDNO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ IDNO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ IDNO:119基本上相同，并且保留SEQ ID NO:1、SEQ ID NO:3或SEQ ID NO:5的蛋白质的功能活性，但是氨基酸序列不同，例如由于天然等位基因变异或诱变而不同。在一些实施方案中，磷酸乙酰转移酶蛋白质包含SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ ID NO:93、SEQID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ IDNO:117或SEQ ID NO:119所示的氨基酸序列。
第二遗传修饰可以是使用第二核酸的转化，并且第二核酸可编码磷酸乙酰转移酶蛋白质，即第二核酸可包含编码磷酸乙酰转移酶蛋白质的第二核苷酸序列。
在一些实施方案中，第二核苷酸序列与SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:34、SEQ ID NO:36、SEQ ID NO:38、SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:44、SEQ ID NO:46、SEQ ID NO:48、SEQ ID NO:50、SEQ ID NO:52、SEQ ID NO:54、SEQ IDNO:56、SEQ ID NO:58、SEQ ID NO:60、SEQ ID NO:62、SEQ ID NO:64、SEQ ID NO:66、SEQ IDNO:68、SEQ ID NO:70、SEQ ID NO:72、SEQ ID NO:74、SEQ ID NO:76、SEQ ID NO:78、SEQ IDNO:80、SEQ ID NO:82、SEQ ID NO:84、SEQ ID NO:86、SEQ ID NO:88、SEQ ID NO:90、SEQ IDNO:92、SEQ ID NO:94、SEQ ID NO:96、SEQ ID NO:98、SEQ ID NO:100、SEQ ID NO:102、SEQID NO:104、SEQ ID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ ID NO:118或SEQ ID NO:120所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性。例如，第二核苷酸序列可具有SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:34、SEQ IDNO:36、SEQ ID NO:38、SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:44、SEQ ID NO:46、SEQ IDNO:48、SEQ ID NO:50、SEQ ID NO:52、SEQ ID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ IDNO:60、SEQ ID NO:62、SEQ ID NO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ IDNO:72、SEQ ID NO:74、SEQ ID NO:76、SEQ ID NO:78、SEQ ID NO:80、SEQ ID NO:82、SEQ IDNO:84、SEQ ID NO:86、SEQ ID NO:88、SEQ ID NO:90、SEQ ID NO:92、SEQ ID NO:94、SEQ IDNO:96、SEQ ID NO:98、SEQ ID NO:100、SEQ ID NO:102、SEQ ID NO:104、SEQ ID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ IDNO:118或SEQ ID NO:120所示的序列。在一些实施方案中，第二核苷酸序列与SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:34、SEQ ID NO:36、SEQ ID NO:38、SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:44、SEQ ID NO:46、SEQ ID NO:48、SEQ ID NO:50、SEQ ID NO:52、SEQ ID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ ID NO:60、SEQ ID NO:62、SEQ IDNO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ ID NO:72、SEQ ID NO:74、SEQ IDNO:76、SEQ ID NO:78、SEQ ID NO:80、SEQ ID NO:82、SEQ ID NO:84、SEQ ID NO:86、SEQ IDNO:88、SEQ ID NO:90、SEQ ID NO:92、SEQ ID NO:94、SEQ ID NO:96、SEQ ID NO:98、SEQ IDNO:100、SEQ ID NO:102、SEQ ID NO:104、SEQ ID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ ID NO:118或SEQ ID NO:120所示的序列基本上相同，并且第二核苷酸序列编码编码保留由SEQ ID NO:1、SEQ ID NO:3、SEQ IDNO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ IDNO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ IDNO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ IDNO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ IDNO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ IDNO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117、or SEQ ID NO:119编码的蛋白质的活性的磷酸乙酰转移酶蛋白质，但是核苷酸序列与SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ ID NO:34、SEQ ID NO:36、SEQ ID NO:38、SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:44、SEQ IDNO:46、SEQ ID NO:48、SEQ ID NO:50、SEQ ID NO:52、SEQ ID NO:54、SEQ ID NO:56、SEQ IDNO:58、SEQ ID NO:60、SEQ ID NO:62、SEQ ID NO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ IDNO:70、SEQ ID NO:72、SEQ ID NO:74、SEQ ID NO:76、SEQ ID NO:78、SEQ ID NO:80、SEQ IDNO:82、SEQ ID NO:84、SEQ ID NO:86、SEQ ID NO:88、SEQ ID NO:90、SEQ ID NO:92、SEQ IDNO:94、SEQ ID NO:96、SEQ ID NO:98、SEQ ID NO:100、SEQ ID NO:102、SEQ ID NO:104、SEQID NO:106、SEQ ID NO:108、SEQ ID NO:110、SEQ ID NO:112、SEQ ID NO:114、SEQ ID NO:116、SEQ ID NO:118或SEQ ID NO:120所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，第二核苷酸序列编码与SEQ ID NO:1、SEQ ID NO:3、SEQ IDNO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ IDNO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ IDNO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ IDNO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ IDNO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ IDNO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，第二核苷酸序列可编码SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ IDNO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ IDNO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ IDNO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ IDNO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ IDNO:119所示的氨基酸序列。在一些实施方案中，第二核苷酸序列编码与SEQ ID NO:1、SEQID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ IDNO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ IDNO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ IDNO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ IDNO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ IDNO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ IDNO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117、or SEQ ID NO:119所示的序列基本上相同的氨基酸序列，并且第二核苷酸编码保留由SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ IDNO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ IDNO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ IDNO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117、or SEQ ID NO:119编码的蛋白质的活性的磷酸乙酰转移酶蛋白质，但是第二核苷酸序列编码与SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ ID NO:65、SEQ ID NO:67、SEQID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ ID NO:77、SEQ ID NO:79、SEQID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:89、SEQ ID NO:91、SEQID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ IDNO:115、SEQ ID NO:117或SEQ ID NO:119所示的序列不同，例如由于天然等位基因变异或诱变而不同。
c.提高乙酸激酶蛋白质的活性
在一些实施方案中，乙酸激酶蛋白质通过酶学委员会编号EC 2.7.2.1分类。
乙酸激酶蛋白质可包含与SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQ IDNO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137或SEQ ID NO:139所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，乙酸激酶蛋白质可与SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQ IDNO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137或SEQ ID NO:139基本上相同，并且保留SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQ ID NO:129、SEQID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137或SEQ ID NO:139的蛋白质的功能活性，但是氨基酸序列不同，例如由于天然等位基因变异或诱变而不同。在一些实施方案中，乙酸激酶蛋白质包含SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137或SEQ ID NO:139所示的氨基酸序列。
第三遗传修饰可以是使用第三核酸的转化，并且第三核酸可编码乙酸激酶蛋白质，即第三核酸可包含编码乙酸激酶蛋白质的第三核苷酸序列。
在一些实施方案中，第三核苷酸序列与SEQ ID NO:14、SEQ ID NO:16、SEQ ID NO:128、SEQ ID NO:130、SEQ ID NO:132、SEQ ID NO:134、SEQ ID NO:136、SEQ ID NO:138或SEQ ID NO:140所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性。例如，第三核苷酸序列可具有SEQ ID NO:14、SEQ ID NO:16、SEQ ID NO:128、SEQ ID NO:130、SEQ ID NO:132、SEQ ID NO:134、SEQ IDNO:136、SEQ ID NO:138或SEQ ID NO:140所示的序列，在一些实施方案中，第三核苷酸序列与SEQ ID NO:14、SEQ ID NO:16、SEQ ID NO:128、SEQ ID NO:130、SEQ ID NO:132、SEQ IDNO:134、SEQ ID NO:136、SEQ ID NO:138或SEQ ID NO:140基本上相同，并且第三核苷酸序列编码保留由SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137或SEQ ID NO:139编码的蛋白质的活性的乙酸激酶蛋白质，但是第三核苷酸序列与SEQ ID NO:14、SEQ ID NO:16、SEQ ID NO:128、SEQ ID NO:130、SEQ ID NO:132、SEQ ID NO:134、SEQ ID NO:136、SEQ ID NO:138或SEQ ID NO:140所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，第三核苷酸序列编码与SEQ ID NO:13、SEQ ID NO:15、SEQ IDNO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137或SEQ ID NO:139所示的序列或者其中任一个的生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，第三核苷酸序列编码可编码SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQID NO:137或SEQ ID NO:139所示的氨基酸序列。在一些实施方案中，第三核苷酸序列编码与SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ IDNO:133、SEQ ID NO:135、SEQ ID NO:137或SEQ ID NO:139所示的序列基本上相同的氨基酸序列，并且第三核苷酸序列编码保留由SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ ID NO:135、SEQ ID NO:137或SEQ ID NO:139编码的蛋白质的活性的乙酸激酶蛋白质，但是第三核苷酸序列编码与SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:127、SEQ ID NO:129、SEQ ID NO:131、SEQ ID NO:133、SEQ IDNO:135、SEQ ID NO:137或SEQ ID NO:139所示的序列不同，例如由于天然等位基因变异或诱变而不同。
d.提高乙醛脱氢酶蛋白质的活性
在一些实施方案中，转化细胞还包含提高细胞中乙醛脱氢酶蛋白质的活性的遗传修饰。例如，遗传修饰可以是编码乙醛脱氢酶蛋白质的核酸的转化。
在一些实施方案中，所述方法还包括用提高细胞中乙醛脱氢酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可编码乙醛脱氢酶蛋白质。
在一些实施方案中，转化细胞还包含提高细胞中NADP-乙醛脱氢酶蛋白质的活性的遗传修饰。例如，遗传修饰可以是使用编码NADP-乙醛脱氢酶蛋白质的核酸的转化。
在一些实施方案中，所述方法还包括用提高细胞中NADP-乙醛脱氢酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可编码NADP-乙醛脱氢酶蛋白质。
在一些实施方案中，乙醛脱氢酶蛋白质通过酶学委员会编号EC 1.2.1.4分类。
乙醛脱氢酶蛋白质可包含与SEQ ID NO:17所示的序列或其生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，乙醛脱氢酶蛋白质可与SEQ ID NO:17基本上相同，并且保留SEQ ID NO:17的蛋白质的功能活性，但是氨基酸序列不同，例如由于天然等位基因变异或诱变而不同。在一些实施方案中，乙醛脱氢酶蛋白质包含SEQ IDNO:17所示的氨基酸序列。
遗传修饰可以是使用编码乙醛脱氢酶蛋白质的核酸的转化，即所述核酸可包含编码乙醛脱氢酶蛋白质的核苷酸序列。
在一些实施方案中，所述核苷酸序列与SEQ ID NO:18所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性。例如，所述核苷酸序列可具有SEQ ID NO:18所示的序列。在一些实施方案中，所述核苷酸序列与SEQ ID NO:18所示的序列基本上相同，并且所述核苷酸序列编码保留由SEQ ID NO:17编码的蛋白质的活性的乙醛脱氢酶蛋白质，但是所述核苷酸序列与SEQ ID NO:18所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，所述核苷酸序列编码与SEQ ID NO:17所示的序列或其生物活性部分具有80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，所述核苷酸序列可编码SEQID NO:17所示的氨基酸序列。在一些实施方案中，所述核苷酸序列编码与SEQ ID NO:17所示的序列基本上相同的氨基酸序列，并且所述核苷酸序列编码保留由SEQ ID NO:17编码的蛋白质的活性的乙醛脱氢酶蛋白质，但是所述核苷酸序列编码与SEQ ID NO:17所示的序列不同，例如由于天然等位基因变异或诱变而不同。
e.降低丙酮酸脱氢酶复合蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然丙酮酸脱氢酶复合蛋白质的活性的遗传修饰，例如，细胞可包含天然丙酮酸脱氢酶复合基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然丙酮酸脱氢酶复合蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然丙酮酸脱氢酶复合基因中的核苷酸序列和/或天然丙酮酸脱氢酶复合基因的调控区中的核苷酸序列重组。
丙酮酸脱氢酶复合蛋白质可通过酶学委员会编号EC 1.2.4.1、EC 2.3.1.12或EC1.8.1.4分类。
f.降低ATP:柠檬酸裂合酶蛋白质的活性
在一些实施方案中，转化细胞还包含降低细胞中天然ATP:柠檬酸裂解酶蛋白质的活性的遗传修饰，例如细胞可包含天然ATP:柠檬酸裂合酶激酶基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然ATP:柠檬酸裂合酶蛋白质的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然ATP:柠檬酸裂合酶酶基因中的核苷酸序列和/或天然ATP:柠檬酸列合酶基因的调控区中的核苷酸序列重组。
ATP：柠檬酸裂合酶蛋白质可通过酶学委员会编号EC 2.3.3.8分类。
D.包含柠檬酸/草酰乙酸线粒体转运蛋白活性、细胞溶质苹果酸酶活性和细胞溶
质丙酮酸羧化酶活性的细胞
提高细胞中柠檬酸/草酰乙酸线粒体转运蛋白、细胞溶质苹果酸酶和细胞溶质丙酮酸羧化酶的活性允许细胞更有效地由细胞溶质柠檬酸合成脂肪酸(图5)。
在一些实施方案中，本发明涉及一种转化细胞，其包含第一遗传修饰、第二遗传修饰和第三遗传修饰，其中所述第一遗传修饰提高细胞中柠檬酸/草酰乙酰线粒体转运蛋白的活性，所述第二遗传修饰提高细胞中细胞溶质苹果酸酶蛋白质的活性，并且所述第三遗传修饰提高细胞中细胞溶质丙酮酸羧化酶蛋白质的活性。在一些实施方案中，转化细胞由细胞溶质柠檬酸合成脂肪酸。在一些实施方案中，转化细胞不由细胞溶质柠檬酸合成脂肪酸，例如，细胞可提高其由其他底物的脂肪酸产生；或者例如细胞可用作用于分子克隆的宿主细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码柠檬酸/草酰乙酸线粒体转运蛋白的第一核苷酸序列转化所述细胞，用编码细胞溶质苹果酸酶蛋白质的第二核苷酸序列转化所述细胞，以及用编码细胞溶质丙酮酸羧化酶蛋白质的第三核苷酸序列转化所述细胞。在一些实施方案中，所述方法通过使细胞能够通过由在糖酵解期间产生的细胞溶质NADH产生细胞溶质NADPH而更有效地由细胞溶质柠檬酸产生脂肪酸来提高细胞的脂质含量。在天然途径中，糖酵解NADH在苹果酸/柠檬酸交换期间穿过线粒体膜被输入到线粒体中，在一些实施方案中，所述方法通过除由细胞溶质柠檬酸产生脂肪酸之外的分子途径来提高细胞的脂质含量，例如细胞溶质柠檬酸增加可提高由不同底物产生脂质，或者细胞溶质柠檬酸增加可降低脂质的降解。
a.提高柠檬酸/草酰乙酸线粒体转运蛋白的活性
柠檬酸/草酰乙酸线粒体转运蛋白可包含与SEQ ID NO:19所示的序列或其生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，柠檬酸/草酰乙酸线粒体转运蛋白可与SEQ ID NO:19基本上相同，并且保留SEQ ID NO:19的蛋白质的功能活性，但氨基酸序列不同，例如由于天然等位基因变异或诱变而不同。在一些实施方案中，柠檬酸/草酰乙酸线粒体转运蛋白包含SEQ ID NO:19所示的氨基酸序列。
第一种遗传修饰可以是使用第一核酸的转化，并且第一核酸可编码柠檬酸/草酰乙酸线粒体转运蛋白，即第一核酸可包含编码柠檬酸/草酰乙酸线粒体转运蛋白的第一核苷酸序列。
在一些实施方案中，第一核苷酸序列与SEQ ID NO:20所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性。例如，第一核苷酸序列可具有SEQ ID NO:20所示的序列。在一些实施方案中，第一核苷酸序列与SEQ ID NO:20所示的序列基本上相同，并且第一核苷酸序列编码保留由SEQ ID NO:19编码的蛋白质的活性的柠檬酸/草酰乙酸线粒体转运蛋白，但是第一核苷酸序列与SEQ ID NO:20所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，第一核苷酸序列编码与SEQ ID NO:19所示的序列或其生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，第一核苷酸序列可编码SEQ ID NO:19所示的氨基酸序列。在一些实施方案中，第一核苷酸序列编码与SEQ IDNO:19所示的序列基本上相同的氨基酸序列，并且第一核苷酸序列编码保留由SEQ ID NO:19编码的蛋白质的活性的柠檬酸/草酰乙酸线粒体转运蛋白，但是第一核苷酸序列编码与SEQ ID NO:19所示的序列不同，例如由于天然等位变异或诱变而不同。
b.提高细胞溶质苹果酸酶蛋白质的活性
在一些实施方案中，细胞溶质苹果酸酶蛋白质通过酶学委员会编号EC 1.1.1.39或1.1.1.40分类。
第二遗传修饰可以是使用第二核酸的转化，并且第二核酸可编码细胞溶质苹果酸酶蛋白质，即第二核酸可包含编码细胞溶质苹果酸酶蛋白质的第二核苷酸序列。
c.提高细胞溶质丙酮酸羧化酶蛋白质的活性
第三遗传修饰可以是使用第三核酸的转化，并且第三核酸可编码细胞溶质丙酮酸羧化酶蛋白质，即第三核酸可包含编码细胞溶质丙酮酸羧化酶蛋白质的第三核苷酸序列。
d.降低柠檬酸/苹果酸线粒体转运蛋白的活性
在一些实施方案中，转化细胞还包含降低细胞中天然柠檬酸/苹果酸线粒体转运蛋白的活性的遗传修饰，例如细胞可包含天然柠檬酸/苹果酸粒体转运蛋白复合基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然柠檬酸/苹果酸线粒体转运蛋白的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然柠檬酸/苹果酸线粒体转运蛋白基因中的核苷酸序列和/或天然柠檬酸/苹果酸线粒体转运蛋白基因的调控区中的核苷酸序列重组。
E.包含柠檬酸/草酰乙酸线粒体转运蛋白活性和烯酰基酰基载体还原酶活性的细
胞
在细胞中提高柠檬酸/草酰乙酸线粒体转运蛋白和烯酰基酰基载体还原酶的活性允许细胞更有效地由细胞溶质柠檬酸合成脂肪酸(图6)。
在一些实施方案中，本发明涉及包含一种转化细胞，其包含第一遗传修饰和第二遗传修饰，其中所述第一遗传修饰提高细胞中柠檬酸/草酰乙酸线粒体转运蛋白的活性，并且所述第二遗传修饰提高细胞的烯酰基酰基载体还原酶活性。在一些实施方案中，转化细胞由细胞溶质柠檬酸合成脂肪酸。在一些实施方案中，转化细胞不由细胞溶质柠檬酸合成脂肪酸，例如细胞可提高其由其他底物的脂肪酸产生；或者例如细胞可用作用于分子克隆的宿主细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码柠檬酸/草酰乙酸线粒体转运蛋白的第一核苷酸序列转化所述细胞，以及用第二核苷酸序列转化所述细胞。第二核苷酸序列可编码烯酰基酰基载体还原酶蛋白质，或者第二核苷酸序列可以能够与天然I型脂肪酸合酶烯酰还原酶基因中的核苷酸序列重组；并且用第二核苷酸序列转化细胞可提高细胞的烯酰基酰基载体还原酶活性。在一些实施方案中，所述方法通过使细胞能够由细胞溶质柠檬酸更高效地产生脂肪酸来提高细胞的脂质含量。在一些实施方案中，所述方法通过除由细胞溶质柠檬酸产生脂肪酸之外的分子途径来提高细胞的脂质含量，例如细胞溶质柠檬酸增加可提高由不同底物产生脂质，或者细胞溶质柠檬酸增加可降低脂质降解。
在一些实施方案中，本发明涉及包含一种转化细胞，其包含第一遗传修饰和第二遗传修饰，其中所述第一遗传修饰提高细胞中柠檬酸/草酰乙酸线粒体转运蛋白的活性，并且所述第二遗传修饰提高细胞的NADH特异性烯酰基酰基载体还原酶活性。在一些实施方案中，转化细胞由细胞溶质柠檬酸合成脂肪酸。在一些实施方案中，转化细胞不由细胞溶质柠檬酸合成脂肪酸，例如，细胞可提高其由其他底物的脂肪酸产生；或者例如细胞可用作用于分子克隆的宿主细胞。
在一些实施方案中，本发明涉及提高细胞的脂质含量的方法，其包括用编码柠檬酸/草酰乙酸线粒体转运蛋白的第一核苷酸序列转化所述细胞，以及用第二核苷酸序列转化所述细胞。第二核苷酸序列可编码NADH特异性烯酰基酰基载体还原酶蛋白质，或者第二核苷酸序列可与天然I型脂肪酸合酶烯酰还原酶基因中的核苷酸序列重组；并且用第二核苷酸序列转化细胞可提高细胞的NADH特异性烯酰基酰基载体还原酶活性。在一些实施方案中，所述方法通过使细胞能够由细胞溶质柠檬酸更高效地产生脂肪酸来提高细胞的脂质含量。在一些实施方案中，所述方法通过除由细胞溶质柠檬酸产生脂肪酸之外的分子途径来提高细胞的脂质含量，例如增细胞溶质柠檬酸增加可提高由不同底物产生脂质，或者细胞溶质柠檬酸增加可降低脂质降解。
a.提高柠檬酸/草酰乙酸线粒体转运蛋白的活性
柠檬酸/草酰乙酸线粒体转运蛋白可包含与SEQ ID NO:19所示的序列或其生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，柠檬酸/草酰乙酸线粒体转运蛋白可与SEQ ID NO:19基本上相同，并且保留SEQ ID NO:19的蛋白质的功能活性，但氨基酸序列不同，例如由于天然等位基因变异或诱变而不同。在一些实施方案中，柠檬酸/草酰乙酸线粒体转运蛋白包含SEQ ID NO:19所示的氨基酸序列。
第一遗传修饰可以是使用第一核酸的转化，并且第一核酸可编码柠檬酸/草酰乙酸线粒体转运蛋白，即第一核酸可包含编码柠檬酸/草酰乙酸线粒体转运蛋白的第一核苷酸序列。
在一些实施方案中，第一核苷酸序列与SEQ ID NO:20所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性。例如，第一核苷酸序列可具有SEQ ID NO:20所示的序列。在一些实施方案中，第一核苷酸序列与SEQ ID NO:20所示的序列基本上相同，并且第一核苷酸序列编码保留由SEQ ID NO:19编码的蛋白质的活性的柠檬酸/草酰乙酸线粒体转运蛋白，但是第一核苷酸序列与SEQ ID NO:20所示的序列不同，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，第一核苷酸序列编码与SEQ ID NO:19所示的序列或其生物活性部分具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更高序列同源性的氨基酸序列。例如，第一核苷酸序列可编码SEQ ID NO:19所示的氨基酸序列。在一些实施方案中，第一核苷酸序列编码与SEQ IDNO:19所示的序列基本上相同的氨基酸序列，并且第一核苷酸序列编码保留由SEQ ID NO:19编码的蛋白质的活性的柠檬酸/草酰乙酸线粒体转运蛋白，但是第一核苷酸序列编码与SEQ ID NO:19所示的序列不同，例如由于天然等位变异或诱变而不同的氨基酸序列。
b.提高烯酰基酰基还原酶活性
在一些实施方案中，第二遗传修饰可以是使用第二核酸的转化，并且第二核酸可编码烯酰基酰基载体还原酶蛋白质，即第二核酸可包含编码烯酰基酰基载体还原酶蛋白质的第二核苷酸序列。
在一些实施方案中，第二遗传修饰可以是使用第二核酸的转化，并且第二核酸可编码NADH特异性烯酰基酰基载体还原酶蛋白质，即第二核酸可包含编码NADH特异性烯酰基酰基还原酶蛋白质的第二核苷酸序列。
NADH特异性烯酰基酰基还原酶蛋白质可通过酶学委员会编号EC 1.3.1.9分类。
在一些实施方案中，第二遗传修饰是天然I型脂肪酸合酶烯酰还原酶蛋白质的突变，其中所述突变提高天然I型脂肪酸合酶烯酰还原酶蛋白质作为电子供体接受NADH的能力。
在一些实施方案中，所述方法包括用第二核苷酸序列转化细胞，所述第二核苷酸序列能够与天然I型脂肪酸合酶烯酰还原酶基因中的核苷酸序列重组以产生提高天然I型脂肪酸合酶烯酰还原酶蛋白质基因产物作为电子供体接受NADH的能力的突变。
c.降低柠檬酸/苹果酸线粒体转运蛋白的活性
在一些实施方案中，转化细胞还包含降低细胞中天然柠檬酸/苹果酸线粒体转运蛋白的活性的遗传修饰，例如细胞可包含天然柠檬酸/苹果酸线粒体转运蛋白复合基因中的敲除突变。
在一些实施方案中，所述方法还包括用降低细胞中天然柠檬酸/苹果酸线粒体转运蛋白的活性的核苷酸序列转化细胞。例如，所述核苷酸序列可以能够与天然柠檬酸/苹果酸线粒体转运蛋白基因中的核苷酸序列和/或天然柠檬酸/苹果酸线粒体转运蛋白基因的调控区中的核苷酸序列重组。
F.产品
在一些方面，本发明涉及一种生产产品的方法，其包括提供转化细胞以及在底物上培养所述细胞一段时间，从而生产所述产品。
底物可包含解聚的糖用甜菜浆、甘油、黑液、玉米、玉米淀粉、玉米糊精、解聚的纤维素材料、玉米秸秆、糖用甜菜浆、柳枝稷、乳清、糖蜜、马铃薯、稻、高粱、甘蔗、稠甘蔗汁、糖用甜菜汁和/或小麦。在某些实施方案中，在存在外源性脂肪酸、葡萄糖、乙醇、木糖、蔗糖、淀粉、淀粉糊精、甘油、纤维素和/或乙酸下培养转化细胞。这些化合物可在培养期间添加到底物中以提高脂质生产。外源性脂肪酸可包括硬脂酸酯/硬脂酸、油酸、亚油酸、γ-亚麻酸、二高-γ-亚麻酸、花生四烯酸、α-亚麻酸、十八碳四烯酸、二十碳四烯酸、二十碳五烯酸、二十二碳五烯酸、二十碳二烯酸和/或二十碳三烯酸。
在某些实施方案中，本发明涉及通过本文所述的经修饰宿主细胞生产的产品。在某些实施方案中，所述产品是油、脂质、脂肪酸、脂肪醇、三酰基甘油酯、类异戊二烯或法呢烯。在一些实施方案中，所述产品是硬脂酸、油酸、亚油酸、癸酸、辛酸、己酸、月桂酸、豆蔻酸、棕榈酸、棕榈油酸或角鲨烯。在某些实施方案中，所述产品是饱和脂肪酸。因此，所述产品可以是辛酸、癸酸、月桂酸、豆蔻酸、棕榈酸、硬脂酸、花生酸、山嵛酸、木蜡酸或蜡酸。在一些实施方案中，所述产品是不饱和脂肪酸。因此，所述产品可以是豆蔻酸、棕榈油酸、顺十六碳烯酸、油酸、反油酸、异油酸、亚油酸、亚反油酸、α-亚麻酸、花生四烯酸、二十碳五烯酸、芥酸或二十二碳六烯酸。
在一些实施方案中，所述产品包含18-碳脂肪酸。在一些实施方案中。所述产品包含油酸、硬脂酸或亚油酸。例如，所述产品可以是油酸。
在一些实施方案中，所述方法包括收集产品。所述方法可包括纯化产品，例如使一个或更多个转化细胞的培养物的脂质级分与一个或更多个该培养物的水性级分分离。
本领域技术人员将容易地理解，本发明很好地适用于实现所提及的目的并获得所提及的终产品和优点以及其中固有的那些。本文描述的实施方案不旨在限制本发明的范围。
实施例
实施例1-在酵母中表达磷酸转酮酶
将来自里氏木霉的磷酸转酮酶基因(NG306；SEQ ID NO:28)克隆到酿酒酵母菌株NS20中。将来自里氏木霉(NG306；SEQ ID NO:28)和黑曲霉(NG304；SEQ ID NO:24)的磷酸转酮酶基因单独克隆到Arxula酵母NS252菌株中。
实施例2-在酵母中表达磷酸乙酰转移酶
将构建体工程化为在酵母中过表达来自枯草芽孢杆菌的磷酸乙酰转移酶基因(NG321；SEQ ID NO:116)(图7)。使用类似的构建体在酵母中过表达来自以下的磷酸乙酰转移酶基因：丙酮丁醇梭菌(Clostridium acetobutylicum)菌株ATCC 824(NG322；SEQ IDNO:118)、解糖热厌氧杆菌(Thermoanaerobacterium saccharolyticum)(NG324；SEQ IDNO:120)、嗜热甲烷八叠球菌(Methanosarcina thermophila)(NG309；SEQ ID NO:2)、巴氏甲烷八叠球菌菌株Fusaro(Methanosarcina barkeri strain Fusaro)(NG310；SEQ ID NO:4)、乙酸甲烷八叠球菌(Methanosarcina acetivorans)(NG311；SEQ ID NO:6)、螯虾丝囊霉(Aphanomyces astaci)(NG350；SEQ ID NO:34)、真菌媒介丝囊霉(Aphanomyces invadans)(NG351；SEQ ID NO:36)、原壳小球藻(Auxenochlorella protothecoides)(NG352；SEQ IDNO:38)、巴西安白僵菌(Beauveria bassiana)(NG353；SEQ ID NO:40)、莱茵衣藻(Chlamydomonas reinhardtii)(NG354；SEQ ID NO:42)、蓝隐藻(Guillardia theta)(NG356；SEQ ID NO:46)、螺旋孢子虫(Helicosporidium)(NG357；SEQ ID NO:48)、海水派金虫(Perkinsus marinus)(NG358；SEQ ID NO:50)、寄生疫霉(Phytophthora parasitica)(NG361；SEQ ID NO:56)、橡树疫霉(Phytophthora ramorum)(NG362；SEQ ID NO:58)、大豆疫霉(Phytophthora sojae)(NG363；SEQ ID NO:60)、终极腐霉(Pythium ultimum)(NG364；SEQ ID NO:62)、异株水霉(Saprolegnia diclina)(NG365；SEQ ID NO:64)、江南卷柏(Selaginella moellendorffii)(NG367；SEQ ID NO:68)、强壮团藻(Volvox carteri)(NG369；SEQ ID NO:72)、枯草芽孢杆菌枯草亚种(Bacillus subtilis subsp.subtilis)菌株168(NG370；SEQ ID NO:74)和解糖热厌氧杆菌(Thermoanaerobacteriumsaccharolyticum)(NG371；SEQ ID NO:76)。
使用构建体转化酿酒酵母菌株NS20、解脂耶氏酵母菌株NS18和Arxula酵母菌株NS252。使用Bock等(J.Bacteriology 181(6):1861-67(1999))所述的测定筛选转化体的磷酸乙酰转移酶活性。简言之，使用Plus可透析酵母蛋白质提取试剂(PierceBiotechnologies,目录#78999)由5ml的过夜YPD培养物制备无细胞提取物。通过以3000rpm离心3分钟使细胞沉淀，并通过涡旋将其重悬于125μl至250μl Y-PER试剂/50m细胞。每1mLY-PER Plus/细胞混合物添加5μl Thermo ScientificTMPierceTM蛋白质酶抑制剂(目录#PI-36978)。搅拌混合物并在室温下静置20分钟。通过以14,000×g离心10分钟使细胞碎片沉淀，并将上清液储存在-80℃直至使用。
将10μl至80μl无细胞提取物添加到在1mL水中包含终浓度100mM Tris-HCl(pH7.2)、5mM MgCl2、5mM KH2PO4和0.1mM DTNB的乙酰CoA反应混合物中。添加乙酰CoA底物至终浓度为0.1mM，并且在412nm下监测5-硫代-2-硝基苯甲酸的吸光度(ε＝14.5mM-1cm-1)。
与转化有来自里氏木霉的磷酸转酮酶基因的对照酿酒酵母细胞相比，每种转化有磷酸乙酰转移酶基因的酿酒酵母细胞都产生更多的5-硫代-2-硝基苯甲酸(图8)。转化有来自枯草芽孢杆菌(NG321；SEQ ID NO:116)或解糖热厌氧杆菌(NG324；SEQ ID NO:120)的磷酸乙酰转移酶基因的酿酒酵母在磷酸乙酰转移酶测定中表现出优异的性能。通过计算比活性，即通过将5-硫代-2-硝基苯甲酸产生速率除以每份无细胞提取物中蛋白质的量将结果归一化。使用PierceTMCoomassie(Bradford)蛋白质测定试剂盒测量蛋白质浓度。与阴性对照相比，转化有来自枯草芽孢杆菌(NG321；SEQ ID NO:116)或解糖热厌氧杆菌(NG324；SEQID NO:120)的磷酸乙酰转移酶基因的酿酒酵母显示出更高的比活性(图9)。
将来自枯草芽孢杆菌的磷酸乙酰转移酶基因(NG321；SEQ ID NO:116)针对酵母进行密码子优化，得到SEQ ID NO:74(NG370)。将来自解糖热厌氧杆菌的磷酸乙酰转移酶基因(NGA24；SEQ ID NO:120)针对酵母进行密码子优化，得到SEQ ID NO:76(NG371)。
来自转化有来自以下的磷酸乙酰转移酶基因的细胞的酿酒酵母细胞提取物的比活性示于图10中：螯虾丝囊霉(NG350；SEQ ID NO:34)、真菌媒介丝囊霉(NG351；SEQ ID NO:36)、原壳小球藻(NG352；SEQ ID NO:38)、巴西安白僵菌(NG353；SEQ ID NO:40)、莱茵衣藻(NG354；SEQ ID NO:42)、蓝隐藻(NG356；SEQ ID NO:46)、螺旋孢子虫(NG357；SEQ ID NO:48)、海水派金虫(NG358；SEQ ID NO:50)、寄生疫霉(NG361；SEQ ID NO:56)、橡树疫霉(NG362；SEQ ID NO:58)、大豆疫霉(NG363；SEQ ID NO:60)、终极腐霉(NG364；SEQ ID NO:62)、异株水霉(NG365；SEQ ID NO:64)、江南卷柏(NG367；SEQ ID NO:68)或强壮团藻(NG369；SEQ ID NO:72)。
用来自枯草芽孢杆菌(NG321；SEQ ID NO:116)、丙酮丁醇梭菌菌株ATCC 824(NG322；SEQ ID NO:118)、解糖热厌氧杆菌(NG324；SEQ ID NO:120)或巴氏甲烷八叠球菌菌株Fusaro(NG310；SEQ ID NO:4)的磷酸乙酰转移酶基因转化Arxula酵母菌株NS252，并如上所述监测磷酸乙酰转移酶比活性(图11)。测定转化有来自里氏木霉(NG306；SEQ ID NO:28)和黑曲霉(NG304；SEQ ID NO:24)的磷酸转酮酶基因的菌株NS252作为阴性对照。来自枯草芽孢杆菌的磷酸乙酰转移酶(NG321；SEQ ID NO:116)显示出最高的活性。
用来自以下的磷酸乙酰转移酶基因转化Arxula酵母菌株NS252，并如上所述监测磷酸乙酰转移酶比活性(图12)：螯虾丝囊霉(NG350；SEQ ID NO:34)、真菌媒介丝囊霉(NG351；SEQ ID NO:36)、原壳小球藻(NG352；SEQ ID NO:38)、巴西安白僵菌(NG353；SEQ IDNO:40)、莱茵衣藻(NG354；SEQ ID NO:42)、蓝隐藻(NG356；SEQ ID NO:46)、螺旋孢子虫(NG357；SEQ ID NO:48)、海水派金虫(NG358；SEQ ID NO:50)、寄生疫霉(NG361；SEQ ID NO:56)、橡树疫霉(NG362；SEQ ID NO:58)、大豆疫霉(NG363；SEQ ID NO:60)、终极腐霉(NG364；SEQ ID NO:62)、异株水霉(NG365；SEQ ID NO:64)、江南卷柏(NG367；SEQ ID NO:68)或强壮团藻(NG369；SEQ ID NO:72)、枯草芽孢杆菌枯草亚种菌株168(NG370；SEQ ID NO:74)或解糖热厌氧杆菌(NG371；SEQ ID NO:76)。使用类似于pNC582的构建体(图7)每种基因被转化到Arxula酵母中，并受控于来自解脂耶氏酵母的PR4启动子，在所述构建体中用磷酸乙酰转移酶基因替代NG321基因。分析每种构建体的四个转化体。来自枯枯草芽孢杆菌枯草亚种菌株168的磷酸乙酰转移酶(NG370；SEQ ID NO:74)显示出最高的活性。
用来自以下的磷酸乙酰转移酶基因转化解脂耶氏酵母菌株NS18，并如上所述监测磷酸乙酰转移酶比活性(图13)：螯虾丝囊霉(NG350；SEQ ID NO:34)、真菌媒介丝囊霉(NG351；SEQ ID NO:36)、原壳小球藻(NG352；SEQ ID NO:38)、巴西安白僵菌(NG353；SEQ IDNO:40)、莱茵衣藻(NG354；SEQ ID NO:42)、枯草芽孢杆菌枯草亚种菌株168(NG370；SEQ IDNO:74)或解糖热厌氧杆菌(NG371；SEQ ID NO:76)。来自枯草芽孢杆菌枯草亚种菌株168(NG370；SEQ ID NO:74)或解糖热厌氧杆菌(NG371；SEQ ID NO:76)的磷酸乙酰转移酶显示出最高活性。
用来自以下的磷酸乙酰转移酶基因转化解脂耶氏酵母菌株NS18，并如上所述监测磷酸乙酰转移酶比活性(图14)：海水派金虫(NG358；SEQ ID NO:50)、寄生疫霉(NG361；SEQID NO:56)、橡树疫霉(NG362；SEQ ID NO:58)、大豆疫霉(NG363；SEQ ID NO:60)、终极腐霉(NG364；SEQ ID NO:62)、异株水霉(NG365；SEQ ID NO:64)、江南卷柏(NG367；SEQ ID NO:68)或强壮团藻(NG369；SEQ ID NO:72)
实施例3-在酵母中缺失6-磷酸果糖激酶基因
解脂耶氏酵母中的PFK1基因编码6-磷酸果糖激酶1蛋白质PFK1(SEQ ID NO:141)。SEQ ID NO:142包含PFK1核苷酸序列、100个上游核苷酸和100个下游核苷酸，并且该序列用于设计能够与天然解脂耶氏酵母6-磷酸果糖激酶1基因重组以产生PFK1敲除的核酸。
包含SEQ ID NO:143和SEQ ID NO:144所示的核苷酸序列的敲除盒各自包含诺尔丝菌素抗性基因Nat的一部分作为标记。SEQ ID NO:144还包含赋予针对5-氟-2’-脱氧尿苷(FUdR)的敏感性的阴性选择标记基因胸苷激酶(TDK)。SEQ ID NO:143和SEQ ID NO:144二者都不编码功能蛋白质，但是这两个序列在成功重组之后能够编码赋予诺尔丝菌素抗性的功能蛋白质。此外，SEQ ID NO:143不包含启动子，并且SEQ ID NO:144不包含终止子，因此其依赖于与解脂耶氏酵母PFK1基因的同源重组来使Nat基因和TDK基因被转录和翻译。以此方式，可以通过在包含诺尔丝菌素的培养基上培养细胞来选择成功转化的细胞。另外，转化细胞由于功能性TDK基因而不能在含有FUdR的培养基中生长。添加TDK基因作为阴性选择标记有利于在后续转化期间去除敲除盒，即成功移除敲除盒意味着新转化的细胞能够在含有5-氟-2’-脱氧尿苷的培养基上生长。
用于SEQ ID NO:143的敲除盒通过用引物NP2782(SEQ ID NO:149)和引物NP356(SEQ ID NO:152)扩增诺尔丝菌素抗性基因Nat(SEQ ID NO:146)来制备。用于SEQ ID NO:144的敲除盒(SEQ ID NO:150)通过用引物NP355(SEQ ID NO:151)和引物NP2783扩增诺尔丝菌素抗性抗性基因Nat(SEQ ID NO:146)和FUdR敏感基因TDK(SEQ ID NO:148)来制备。用于这些PCR反应的模板载体是载体pNC468(图15)。可以设计类似的敲除盒来降低Arxula酵母(Arxula adeninivorans)的PFK1基因(SEQ ID NO:154)、Arxula酵母(Arxulaadeninivorans)的PFK2基因(SEQ ID NO:156)或者Arxula或不同物种的另外已知基因的活性。
用于SEQ ID NO:143和SEQ ID NO:144的敲除盒用于从解脂耶氏酵母菌株NS18中缺失PFK1基因。使用PCR来检测完整PFK1基因的存在并检测SEQ ID NO:143和SEQ ID NO:144核苷酸序列的成功重组。引物NP2784(SEQ ID NO:157)与解脂耶氏酵母PFK1基因上游的区域结合，引物NP2785(SEQ ID NO:158)与PFK1基因的内部区域结合，并这两种引物用于确定转化细胞是否包含完整的PFK1基因。图16的图A显示八个转化菌落均不包含完整的PFK1基因，而未转化的阴性对照NG18包含完整的PFK1基因。因此，两个敲除盒成功地缺失每个转化体中的PFK1基因。引物NP2784(SEQ ID NO:157)与PFK1基因上游的区域结合，引物NP356(SEQ ID NO:152)与Nat基因结合，并且这两种引物用于确定转化细胞是否包含敲除盒。图16的图B显示八个转化体中有六个包含整合到PFK1基因座中的敲除盒。包含PFK1缺失和整合敲除盒的菌落1被命名为NS807菌株。
将野生型解脂耶氏酵母菌株NS18和PFK1敲除菌株NS807平板接种在包含葡萄糖作为唯一碳源的基本培养基上。菌株NS18能够在葡萄糖上生长，而NS807菌株则不能，从而确定菌株NS807中的PFK1缺失(图17)。
使用Flores等(Microbiology,151:1465-74(2005))所述的磷酸果糖激酶测定评估野生型解脂耶氏酵母菌株NS18和PFK1敲除菌株NS807的磷酸果糖激酶活性。从每种菌株培养至OD600为0.5至0.6的2L过夜培养物提取蛋白质。在4℃以5000rpm使细胞沉淀，并将其在含有1M山梨醇的20mM HEPES(pH7.6)中洗涤两次。将细胞沉淀物在包含100mM HEPES(pH7.6)的裂解缓冲液中洗涤，HEPES含有0.8M山梨醇、10mM乙酸镁、2mM EDTA和300mM谷氨酸钾，然后悬浮于最小体积的裂解缓冲液(约沉淀物体积的1/3)中。将细胞在液氮中冷冻并储存在-80℃。在咖啡研磨机中使用20秒脉冲用干冰通过研磨15至20次来裂解细胞。将经研磨的干冰/细胞混合物转移到离心管中，并使干冰在室温下升华。在4℃下以22,000rpm持续30分钟使细胞碎片沉淀，将上清液储存在-80℃直至使用。如Flores等(Microbiology,151:1465-74(2005))等所述使用上清液来评估磷酸果糖激酶活性。使用利用PierceTMCoomassie(Bradford)蛋白质测定试剂盒计算的总蛋白质浓度归一化活性。从解脂耶氏酵母中缺失PFK1基因相对于野生型菌株NS18降低了菌株NS807中的PFK活性(图18)。
通过引用并入
本文引用的所有专利、公开的专利申请和非专利文献均在此通过引用并入。
等同方案
本领域技术人员将认识到或能够仅使用常规实验就可确定本文所述的本发明具体实施方案的许多等同方案。这样的等同方案旨在被所附权利要求书涵盖。
序列表
<110> 诺沃吉公司
<120> 增强产油酵母中核心脂质的生产
<130> NGX-038.25
<140>
<141>
<150> 62/097,781
<151> 2014-12-30
<160> 166
<170> PatentIn version 3.5
<210> 1
<211> 333
<212> PRT
<213> Methanosarcina thermophila
<400> 1
Leu Val Thr Phe Leu Glu Lys Ile Ser Glu Arg Ala Lys Lys Leu Asn
1 5 10 15
Lys Thr Ile Ala Leu Pro Glu Thr Glu Asp Ile Arg Thr Leu Gln Ala
20 25 30
Ala Ala Lys Ile Leu Glu Arg Gly Ile Ala Asp Ile Val Leu Val Gly
35 40 45
Asn Glu Ala Asp Ile Lys Ala Leu Ala Gly Asp Leu Asp Leu Ser Lys
50 55 60
Ala Lys Ile Val Asp Pro Lys Thr Tyr Glu Lys Lys Asp Glu Tyr Ile
65 70 75 80
Asn Ala Phe Tyr Glu Leu Arg Lys His Lys Gly Ile Thr Leu Glu Asn
85 90 95
Ala Ala Glu Ile Met Ser Asp Tyr Val Tyr Phe Ala Val Met Met Ala
100 105 110
Lys Leu Gly Glu Val Asp Gly Val Val Ser Gly Ala Ala His Ser Ser
115 120 125
Ser Asp Thr Leu Arg Pro Ala Val Gln Ile Val Lys Thr Ala Lys Gly
130 135 140
Ala Ala Leu Ala Ser Ala Phe Phe Ile Ile Ser Val Pro Asp Cys Glu
145 150 155 160
Tyr Gly Ser Asp Gly Thr Phe Leu Phe Ala Asp Ser Gly Met Val Glu
165 170 175
Met Pro Ser Val Glu Asp Val Ala Asn Ile Ala Val Ile Ser Ala Lys
180 185 190
Thr Phe Glu Leu Leu Val Gln Asp Val Pro Lys Val Ala Met Leu Ser
195 200 205
Tyr Ser Thr Lys Gly Ser Ala Lys Ser Lys Leu Thr Glu Ala Thr Ile
210 215 220
Ala Ser Thr Lys Leu Ala Gln Glu Leu Ala Pro Asp Ile Ala Ile Asp
225 230 235 240
Gly Glu Leu Gln Val Asp Ala Ala Ile Val Pro Lys Val Ala Ala Ser
245 250 255
Lys Ala Pro Gly Ser Pro Val Ala Gly Lys Ala Asn Val Phe Ile Phe
260 265 270
Pro Asp Leu Asn Cys Gly Asn Ile Ala Tyr Lys Ile Ala Gln Arg Leu
275 280 285
Ala Lys Ala Glu Ala Tyr Gly Pro Ile Thr Gln Gly Leu Ala Lys Pro
290 295 300
Ile Asn Asp Leu Ser Arg Gly Cys Ser Asp Glu Asp Ile Val Gly Ala
305 310 315 320
Val Ala Ile Thr Cys Val Gln Ala Ala Ala Gln Asp Lys
325 330
<210> 2
<211> 1002
<212> DNA
<213> Methanosarcina thermophila
<400> 2
ttggtaacat ttttagagaa gatcagtgaa agagcaaaga aacttaacaa aacaatcgct 60
ttacccgaaa ctgaagatat aagaaccctc caggcagctg ccaagatcct tgaaagaggt 120
attgcagaca ttgtccttgt cggtaatgag gcagatatta aggcgctcgc aggagatctg 180
gatctctcaa aagcaaaaat tgtagatcct aaaacctatg agaaaaaaga tgaatacatt 240
aacgctttct acgagttgag aaagcacaaa ggcatcacac tcgaaaatgc agctgaaatc 300
atgagcgatt acgtttactt cgctgttatg atggccaaac tcggggaagt agacggtgta 360
gtatcaggcg ctgcccactc ttcttcagac accctgaggc ctgctgtcca gatcgtgaaa 420
acagccaagg gcgcagctct tgcatccgct ttcttcataa tctctgtgcc tgactgtgaa 480
tatgggtcag atggcacatt ccttttcgct gactctggca tggttgaaat gccgagcgta 540
gaagacgttg caaacattgc agttatttcc gcaaagacct tcgaattgct ggtccaggac 600
gtgccaaagg ttgcaatgct ctcctactcc accaagggaa gcgccaagag caaactgacc 660
gaagcaacaa ttgcttctac aaaacttgca caggaacttg ctcctgatat cgcaattgac 720
ggtgaactcc aggttgacgc cgcgattgtc cccaaagttg cagcttcaaa agcccccgga 780
agccctgttg caggcaaagc caatgtcttc attttccctg acctgaactg tggaaacatc 840
gcatacaaga tcgcccagag gcttgctaaa gctgaagctt atggtcctat cacccaggga 900
ctggcaaagc caattaacga cctgtccaga ggctgcagcg acgaagatat tgtcggtgcc 960
gttgccatta cctgtgtaca ggccgcagca caggacaaat aa 1002
<210> 3
<211> 333
<212> PRT
<213> Methanosarcina barkeri
<400> 3
Met Val Thr Phe Leu Glu Lys Ile Ser Glu Arg Ala Lys Lys Leu Asn
1 5 10 15
Lys Thr Ile Ala Leu Pro Glu Thr Thr Asp Ile Arg Thr Leu Gln Ala
20 25 30
Ala Ala Lys Ala Leu Glu Arg Gly Val Ala Asn Ile Val Leu Ile Gly
35 40 45
Asp Glu Ala Asn Ile Lys Glu Leu Ala Gly Asp Leu Asp Leu Ser Lys
50 55 60
Ala Lys Ile Val Asn Pro Glu Thr Tyr Glu Lys Lys Asp Glu Tyr Ile
65 70 75 80
Gln Ala Phe Tyr Glu Leu Arg Lys His Lys Gly Ile Thr Leu Glu Ser
85 90 95
Ala Ala Glu Ile Met Lys Asp Tyr Val Tyr Phe Ala Val Met Ala Ala
100 105 110
Lys Leu Asn Glu Val Asp Gly Val Val Ser Gly Ala Val His Ser Ser
115 120 125
Ser Asp Thr Leu Arg Pro Ala Val Gln Ile Val Lys Thr Ala Pro Asp
130 135 140
Ala Ala Leu Ala Ser Ala Phe Phe Ile Ile Ala Val Pro Asp Cys Glu
145 150 155 160
Tyr Gly Ser Glu Gly Thr Phe Leu Phe Ala Asp Ser Gly Met Val Glu
165 170 175
Met Pro Ser Pro Glu Asp Val Ala Asn Ile Ala Ile Ile Ser Ala Lys
180 185 190
Thr Phe Glu Leu Leu Val Gln Asp Asp Pro Tyr Val Ala Met Leu Ser
195 200 205
Tyr Ser Thr Lys Gly Ser Ala His Ser Lys Leu Thr Glu Ala Thr Ile
210 215 220
Ala Ala Thr Lys Leu Ala Gln Glu Leu Ala Pro Asp Ile Pro Ile Asp
225 230 235 240
Gly Glu Leu Gln Val Asp Ala Ala Ile Val Pro Lys Val Ala Ala Ser
245 250 255
Lys Ala Pro Gly Ser Pro Val Ala Gly Lys Ala Asn Val Phe Ile Phe
260 265 270
Pro Asp Leu Asn Ala Gly Asn Ile Ala Tyr Lys Ile Ala Gln Arg Leu
275 280 285
Ala Lys Ala Glu Ala Tyr Gly Pro Ile Thr Gln Gly Leu Ala Lys Pro
290 295 300
Ile Asn Asp Leu Ser Arg Gly Cys Ser Asp Glu Asp Ile Val Gly Ala
305 310 315 320
Ile Ala Ile Thr Cys Val Gln Ala Ala Ala Gln Asp Lys
325 330
<210> 4
<211> 1002
<212> DNA
<213> Methanosarcina barkeri
<400> 4
ttggtaacat ttttagaaaa aatcagtgaa agagcaaaga aactcaacaa aacaattgct 60
ttacctgaaa ctaccgatat aagaactctt caggcagctg ccaaggccct cgaaagaggg 120
gttgcaaata tcgttctcat cggcgatgaa gcaaatatta aggagcttgc aggagatctt 180
gacctctcaa aagcaaaaat tgtaaatcct gagacttacg agaaaaagga tgaatacatt 240
caggctttct acgagctgag aaagcataag ggtattacac tcgaaagtgc agccgaaatt 300
atgaaggatt acgtttactt cgctgttatg gcggctaaac tcaatgaagt agacggtgta 360
gtttcaggtg ctgttcactc ttcctctgat acacttagac ctgctgtcca gattgttaaa 420
actgcccctg atgcagctct cgcatctgct tttttcatta ttgccgtgcc ggactgtgaa 480
tatgggtcag aagggacatt cctctttgct gactcaggta tggttgaaat gcccagtcct 540
gaagacgttg caaacattgc tatcatttct gcaaaaacct ttgaactgct ggttcaggat 600
gatccatatg ttgcaatgct ttcttattcc actaagggaa gtgcacacag caaactgact 660
gaggcaacaa ttgctgccac aaagcttgca caggaactcg ctccagatat tccaattgat 720
ggtgaactcc aggtagatgc agcaattgtt ccaaaagttg cagcttcaaa ggctccagga 780
agccctgttg caggcaaagc taatgtcttt atcttccctg accttaacgc tggaaacata 840
gcatacaaga ttgcccagag gctcgccaag gccgaagctt atggccctat tactcaggga 900
cttgccaagc caattaatga cttatccaga ggctgcagcg acgaagacat tgtcggtgct 960
attgcaatta cgtgcgttca ggccgcagca caggacaaat aa 1002
<210> 5
<211> 333
<212> PRT
<213> Methanosarcina acetivorans
<400> 5
Leu Val Thr Phe Leu Glu Lys Ile Ser Glu Arg Ala Lys Lys Leu Asn
1 5 10 15
Lys Thr Ile Ala Leu Pro Glu Thr Glu Asp Ile Arg Thr Leu Gln Ala
20 25 30
Ala Ala Lys Ile Leu Glu Arg Gly Ile Ala Asn Val Val Leu Val Gly
35 40 45
Asp Glu Ala Asp Ile Lys Ala Leu Ala Gly Asp Leu Asp Leu Ser Lys
50 55 60
Ala Lys Ile Val Asn Pro Lys Thr Tyr Glu Lys Lys Asp Glu Tyr Ile
65 70 75 80
Asn Thr Phe Tyr Glu Leu Arg Lys His Lys Gly Ile Thr Pro Glu Thr
85 90 95
Ala Ala Glu Val Met Ser Asp Tyr Val Tyr Phe Ala Val Met Met Ala
100 105 110
Lys Leu Gly Glu Val Asp Gly Val Val Ser Gly Ala Ala His Ser Ser
115 120 125
Ser Asp Thr Leu Arg Pro Ala Val Gln Ile Val Lys Thr Ala Pro Gly
130 135 140
Ala Ala Leu Ala Ser Ala Phe Phe Ile Ile Ala Val Pro Asp Cys Glu
145 150 155 160
Tyr Gly Ser Asp Gly Thr Phe Leu Phe Ala Asp Ser Gly Met Val Glu
165 170 175
Ile Pro Ser Val Glu Asp Val Ala Asn Ile Ala Val Ile Ser Ala Lys
180 185 190
Thr Phe Glu Leu Leu Val Gln Asp Thr Pro Tyr Val Ala Met Leu Ser
195 200 205
Tyr Ser Thr Lys Gly Ser Ala His Ser Lys Leu Thr Glu Ala Thr Val
210 215 220
Ala Ala Thr Lys Leu Ala Gln Glu Leu Ala Pro Asp Val Ala Ile Asp
225 230 235 240
Gly Glu Leu Gln Val Asp Ala Ala Val Val Pro Lys Val Ala Ala Ser
245 250 255
Lys Ala Pro Gly Ser Pro Val Ala Gly Lys Ala Asn Val Phe Ile Phe
260 265 270
Pro Asp Leu Asn Ala Gly Asn Ile Ala Tyr Lys Ile Ala Gln Arg Leu
275 280 285
Ala Lys Ala Glu Ala Tyr Gly Pro Ile Thr Gln Gly Leu Ala Lys Pro
290 295 300
Ile Asn Asp Leu Ser Arg Gly Cys Ser Asp Glu Asp Ile Val Gly Ala
305 310 315 320
Val Ala Ile Thr Cys Val Gln Ala Ala Ala Gln Asp Lys
325 330
<210> 6
<211> 1002
<212> DNA
<213> Methanosarcina acetivorans
<400> 6
ttggtaacat ttttagagaa gatcagtgaa agagcaaaga aactcaacaa gacaatcgct 60
ttacctgaaa ctgaagatat aaggaccctc caggcagctg ccaagatcct tgaaagaggt 120
attgcaaacg ttgtccttgt cggtgatgaa gccgacatta aggcgctcgc aggagatctt 180
gatctctcaa aagcaaaaat tgtaaatccg aaaacctatg agaaaaaaga cgaatacatt 240
aacactttct atgagctgag aaagcacaaa ggtataaccc ccgaaactgc agctgaagtt 300
atgagcgatt acgtttactt cgccgttatg atggcaaaac tcggagaagt agacggagta 360
gtatcaggag ctgctcactc ttcttcagac accctgagac ctgctgtcca gatagttaag 420
actgctccag gcgcagcact tgcatccgct ttcttcatca ttgccgtacc tgactgtgaa 480
tacggatcag atgggacctt cctctttgcc gactcgggta tggttgaaat tccaagtgta 540
gaggatgttg caaacattgc agttatttct gcaaagacct tcgaactgct ggttcaggac 600
actccgtatg ttgcaatgct ttcctattcc accaaaggaa gtgcacacag caaactgacc 660
gaggcaacag tcgctgccac aaagcttgca caggaacttg ctcctgacgt tgcaatcgac 720
ggtgaactgc aggttgatgc agcagttgtc cccaaagttg cagcttcaaa ggctcccgga 780
agccctgtcg caggtaaagc caatgtcttt atcttccccg atctgaacgc tggaaacatc 840
gcatacaaga tcgcccagag gcttgccaag gctgaagcat atggtcctat cacccaggga 900
cttgcaaagc cgattaacga cctgtccaga ggatgcagtg acgaagatat tgtcggtgct 960
gttgcgatta cctgtgtcca ggccgcagcc caggacaaat aa 1002
<210> 7
<211> 549
<212> PRT
<213> Saccharomyces cerevisiae
<400> 7
Met Ser Glu Ile Thr Leu Gly Lys Tyr Leu Phe Glu Arg Leu Lys Gln
1 5 10 15
Val Asn Val Asn Thr Val Phe Gly Leu Pro Gly Asp Phe Asn Leu Ser
20 25 30
Leu Leu Asp Lys Ile Tyr Glu Val Glu Gly Met Arg Trp Ala Gly Asn
35 40 45
Ala Asn Glu Leu Asn Ala Ala Tyr Ala Ala Asp Gly Tyr Ala Arg Ile
50 55 60
Lys Gly Met Ser Cys Ile Ile Thr Thr Phe Gly Val Gly Glu Leu Ser
65 70 75 80
Ala Leu Asn Gly Ile Ala Gly Ser Tyr Ala Glu His Val Gly Val Leu
85 90 95
His Val Val Gly Val Pro Ser Ile Ser Ser Gln Ala Lys Gln Leu Leu
100 105 110
Leu His Thr Leu Gly Asn Gly Asp Phe Thr Val Phe His Arg Met Ser
115 120 125
Ala Asn Ile Ser Glu Thr Thr Ala Met Ile Thr Asp Ile Cys Thr Pro
130 135 140
Gln Ala Glu Ile Asp Arg Cys Ile Arg Thr Thr Tyr Val Thr Gln Arg
145 150 155 160
Pro Val Tyr Leu Gly Leu Pro Ala Asn Leu Val Asp Leu Asn Val Pro
165 170 175
Ala Lys Leu Leu Gln Thr Pro Ile Asp Met Ser Leu Lys Pro Asn Asp
180 185 190
Ala Glu Ser Glu Lys Glu Val Ile Asp Thr Ile Leu Val Leu Val Lys
195 200 205
Asp Ala Lys Asn Pro Val Ile Leu Ala Asp Ala Cys Cys Ser Arg His
210 215 220
Asp Val Lys Ala Glu Thr Lys Lys Leu Ile Asp Leu Thr Gln Phe Pro
225 230 235 240
Ala Phe Val Thr Pro Met Gly Lys Gly Ser Ile Ser Glu Gln His Pro
245 250 255
Arg Tyr Gly Gly Val Tyr Val Gly Thr Leu Ser Lys Pro Glu Val Lys
260 265 270
Glu Ala Val Glu Ser Ala Asp Leu Ile Leu Ser Val Gly Ala Leu Leu
275 280 285
Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Ser Tyr Lys Thr Lys Asn
290 295 300
Ile Val Glu Phe His Ser Asp His Met Lys Ile Arg Asn Ala Thr Phe
305 310 315 320
Pro Gly Val Gln Met Lys Phe Val Leu Gln Lys Leu Leu Thr Asn Ile
325 330 335
Ala Asp Ala Ala Lys Gly Tyr Lys Pro Val Ala Val Pro Ala Arg Thr
340 345 350
Pro Ala Asn Ala Ala Val Pro Ala Ser Thr Pro Leu Lys Gln Glu Trp
355 360 365
Met Trp Asn Gln Leu Gly Asn Phe Leu Gln Glu Gly Asp Val Val Ile
370 375 380
Ala Glu Thr Gly Thr Ser Ala Phe Gly Ile Asn Gln Thr Thr Phe Pro
385 390 395 400
Asn Asn Thr Tyr Gly Ile Ser Gln Val Leu Trp Gly Ser Ile Gly Phe
405 410 415
Thr Thr Gly Ala Thr Leu Gly Ala Ala Phe Ala Ala Glu Glu Ile Asp
420 425 430
Pro Lys Lys Arg Val Ile Leu Phe Ile Gly Asp Gly Ser Leu Gln Leu
435 440 445
Thr Val Gln Glu Ile Ser Thr Met Ile Arg Trp Gly Leu Lys Pro Tyr
450 455 460
Leu Phe Val Leu Asn Asn Asp Gly Tyr Thr Ile Glu Lys Leu Ile His
465 470 475 480
Gly Pro Lys Ala Gln Tyr Asn Glu Ile Gln Gly Trp Asp His Leu Ser
485 490 495
Leu Leu Pro Thr Phe Gly Ala Lys Asp Tyr Glu Thr His Arg Val Ala
500 505 510
Thr Thr Gly Glu Trp Asp Lys Leu Thr Gln Asp Lys Ser Phe Asn Asp
515 520 525
Asn Ser Lys Ile Arg Met Ile Glu Val Met Leu Pro Val Phe Asp Cys
530 535 540
Ser Thr Lys Leu Gly
545
<210> 8
<211> 1650
<212> DNA
<213> Saccharomyces cerevisiae
<400> 8
atgtctgaaa ttactttggg taaatatttg ttcgaaagat taaagcaagt caacgttaac 60
accgttttcg gtttgccagg tgacttcaac ttgtccttgt tggacaagat ctacgaagtt 120
gaaggtatga gatgggctgg taacgccaac gaattgaacg ctgcttacgc cgctgatggt 180
tacgctcgta tcaagggtat gtcttgtatc atcaccacct tcggtgtcgg tgaattgtct 240
gctttgaacg gtattgccgg ttcttacgct gaacacgtcg gtgttttgca cgttgttggt 300
gtcccatcca tctcttctca agctaagcaa ttgttgttgc acaccttggg taacggtgac 360
ttcactgttt tccacagaat gtctgccaac atttctgaaa ccactgctat gatcactgac 420
atctgtacgc cccaggctga aattgacaga tgtatcagaa ccacttacgt cacccaaaga 480
ccagtctact taggtttgcc agctaacttg gtcgacttga acgtcccagc taagttgttg 540
caaactccaa ttgacatgtc tttgaagcca aacgatgctg aatccgaaaa ggaagtcatt 600
gacaccatct tggtcttggt caaggatgct aagaacccag ttattctggc tgatgcttgt 660
tgttccagac acgacgtcaa ggctgaaact aagaagttga ttgacttgac tcaattccca 720
gctttcgtca ccccaatggg taagggttcc attagcgaac aacacccaag atacggtggt 780
gtttacgtcg gtaccttgtc caagccagaa gttaaggaag ccgttgaatc tgctgacttg 840
attttgtctg tcggtgcttt gttgtctgat ttcaacaccg gttctttctc ttactcttac 900
aagaccaaga acattgtcga attccactcc gaccacatga agatcagaaa cgccactttc 960
ccaggtgtcc aaatgaaatt cgttttgcaa aagttgttga ccaatattgc tgacgccgct 1020
aagggttaca agccagttgc tgtcccagct agaactccag ctaacgctgc tgtcccagct 1080
tctaccccat tgaagcaaga atggatgtgg aaccaattgg gtaacttctt gcaagaaggt 1140
gatgttgtca ttgctgaaac cggtacctcc gctttcggta tcaaccaaac cactttccca 1200
aacaacacct acggtatctc tcaagtctta tggggttcca ttggtttcac cactggtgct 1260
accttgggtg ctgctttcgc tgctgaagaa attgatccaa agaagagagt tatcttattc 1320
attggtgacg gttctttgca attgactgtt caagaaatct ccaccatgat cagatggggc 1380
ttgaagccat acttgttcgt cttgaacaac gatggttaca ccattgaaaa gttgattcac 1440
ggtccaaagg ctcaatacaa cgaaattcaa ggttgggacc acctatcctt gttgccaact 1500
ttcggtgcta aggactacga aacccacaga gtcgctacca ccggtgaatg ggacaagttg 1560
acccaagaca agtctttcaa cgacaactct aagatcagaa tgattgaggt tatgttgcca 1620
gtcttcgatt gctccacaaa acttggttga 1650
<210> 9
<211> 563
<212> PRT
<213> Saccharomyces cerevisiae
<400> 9
Met Ser Glu Ile Thr Leu Gly Lys Tyr Leu Phe Glu Arg Leu Ser Gln
1 5 10 15
Val Asn Cys Asn Thr Val Phe Gly Leu Pro Gly Asp Phe Asn Leu Ser
20 25 30
Leu Leu Asn Lys Leu Tyr Glu Val Lys Gly Met Arg Trp Ala Gly Asn
35 40 45
Ala Asn Glu Leu Asn Ala Ala Tyr Ala Ala Asp Gly Tyr Ala Arg Ile
50 55 60
Lys Gly Met Ser Cys Ile Ile Thr Thr Phe Gly Val Gly Glu Leu Ser
65 70 75 80
Ala Leu Asn Gly Ile Ala Gly Ser Tyr Ala Glu His Val Gly Val Leu
85 90 95
His Val Val Gly Val Pro Ser Ile Ser Ser Gln Ala Lys Gln Leu Leu
100 105 110
Leu His His Thr Leu Gly Asn Gly Asp Phe Thr Val Phe His Arg Met
115 120 125
Ser Ala Asn Ile Ser Glu Thr Thr Ala Met Ile Thr Asp Ile Arg Asn
130 135 140
Ala Pro Ala Glu Ile Asp Arg Cys Ile Arg Thr Thr Tyr Thr Thr Gln
145 150 155 160
Arg Pro Val Tyr Leu Gly Leu Pro Ala Asn Leu Val Asp Leu Asn Val
165 170 175
Pro Ala Lys Leu Leu Glu Thr Pro Ile Asp Leu Ser Leu Lys Pro Asn
180 185 190
Asp Ala Glu Ala Glu Ala Glu Val Val Arg Thr Val Val Glu Phe Ile
195 200 205
Lys Asp Ala Lys Asn Pro Val Ile Leu Ala Asp Ala Cys Cys Ser Arg
210 215 220
His Asp Val Lys Ala Glu Thr Lys Lys Leu Met Asp Leu Thr Gln Phe
225 230 235 240
Pro Val Tyr Val Thr Pro Met Gly Lys Gly Ala Ile Asp Glu Gln His
245 250 255
Pro Arg Tyr Gly Gly Val Tyr Val Gly Thr Leu Ser Arg Pro Glu Val
260 265 270
Lys Lys Ala Val Glu Ser Ala Asp Leu Ile Leu Ser Ile Gly Ala Leu
275 280 285
Leu Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Ser Tyr Lys Thr Lys
290 295 300
Asn Ile Val Glu Phe His Ser Asp His Ile Lys Ile Arg Asn Ala Thr
305 310 315 320
Phe Pro Gly Val Gln Met Lys Phe Ala Leu Gln Lys Leu Leu Asp Ala
325 330 335
Ile Pro Glu Val Ala Lys Asp Tyr Lys Pro Val Ala Val Pro Ala Arg
340 345 350
Val Pro Ile Thr Lys Ser Thr Pro Ala Asn Thr Pro Met Lys Gln Glu
355 360 365
Trp Met Trp Asn Gln Leu Gly Asn Phe Leu Arg Glu Gly Asp Ile Val
370 375 380
Ile Ala Glu Thr Gly Thr Ser Ala Phe Gly Ile Asn Gln Thr Thr Phe
385 390 395 400
Pro Thr Asp Val Tyr Ala Ile Val Gln Val Leu Trp Gly Ser Ile Gly
405 410 415
Phe Thr Val Gly Ala Leu Leu Gly Ala Thr Met Ala Ala Glu Glu Leu
420 425 430
Asp Pro Lys Lys Arg Val Ile Leu Phe Ile Gly Asp Gly Ser Leu Gln
435 440 445
Leu Thr Val Gln Glu Ile Ser Thr Met Ile Arg Trp Gly Leu Lys Pro
450 455 460
Tyr Ile Phe Val Leu Asn Asn Asn Gly Tyr Thr Ile Glu Lys Leu Ile
465 470 475 480
His Gly Pro His Ala Glu Tyr Asn Glu Ile Gln Gly Trp Asp His Leu
485 490 495
Ala Leu Leu Pro Thr Phe Gly Ala Arg Asn Tyr Glu Thr His Arg Val
500 505 510
Ala Thr Thr Gly Glu Trp Glu Lys Leu Thr Gln Asp Lys Asp Phe Gln
515 520 525
Asp Asn Ser Lys Ile Arg Met Ile Glu Val Met Leu Pro Val Phe Asp
530 535 540
Ala Pro Gln Asn Leu Val Lys Gln Ala Gln Leu Thr Ala Ala Thr Asn
545 550 555 560
Ala Lys Gln
<210> 10
<211> 1692
<212> DNA
<213> Saccharomyces cerevisiae
<400> 10
atgtctgaaa taaccttagg taaatattta tttgaaagat tgagccaagt caactgtaac 60
accgtcttcg gtttgccagg tgactttaac ttgtctcttt tgaataagct ttatgaagtc 120
aaaggtatga gatgggctgg taacgctaac gaattgaacg ctgcctatgc tgctgatggt 180
tacgctcgta tcaagggtat gtcctgtatt attaccacct tcggtgtcgg tgaattgtct 240
gctttgaatg gtattgccgg ttcttacgct gaacatgtcg gtgttttgca cgttgttggt 300
gttccatcca tctcttctca agctaagcaa ttgttgttgc atcatacctt gggtaacggt 360
gacttcactg ttttccacag aatgtctgcc aacatttctg aaaccactgc catgatcact 420
gatattcgta acgctccagc tgaaattgac agatgtatca gaaccaccta cactacccaa 480
agaccagtct acttgggttt gccagctaac ttggttgact tgaacgtccc agccaagtta 540
ttggaaactc caattgactt gtctttgaag ccaaacgacg ctgaagctga agctgaagtt 600
gttagaactg ttgttgaatt catcaaggat gctaagaacc cagttatctt ggctgatgct 660
tgttgctcta gacatgatgt caaggctgaa actaagaagt tgatggactt gactcaattc 720
ccagtttacg tcaccccaat gggtaagggt gctattgacg aacaacaccc aagatacggt 780
ggtgtttacg ttggtacctt gtctagacca gaagttaaga aggctgtaga atctgctgat 840
ttgatattgt ctatcggtgc tttgttgtct gatttcaaca ccggttcttt ctcttactcc 900
tacaagacca agaacattgt cgaattccac tctgaccaca tcaagatcag aaacgccacc 960
ttcccaggtg ttcaaatgaa atttgccttg caaaaattgt tggatgctat tccagaagtc 1020
gctaaggact acaaaccagt tgctgtccca gctagagttc caattaccaa gtctactcca 1080
gctaacactc caatgaagca agaatggatg tggaaccaat tgggtaactt cttgagagaa 1140
ggtgatattg ttattgctga aaccggtact tccgccttcg gtattaacca aactactttc 1200
ccaacagatg tatacgctat cgtccaagtc ttgtggggtt ccattggttt cacagtcggt 1260
gctctattgg gtgctactat ggccgctgaa gaacttgatc caaagaagag agttatttta 1320
ttcattggtg acggttctct acaattgact gttcaagaaa tctctaccat gattagatgg 1380
ggtttgaagc catacatttt tgtcttgaat aacaacggtt acaccattga aaaattgatt 1440
cacggtcctc atgccgaata taatgaaatt caaggttggg accacttggc cttattgcca 1500
actttcggtg ctagaaacta cgaaacccac agagttgcta ccactggtga atgggaaaag 1560
ttgactcaag acaaggactt ccaagacaac tctaagatta gaatgattga agttatgttg 1620
ccagtctttg atgctccaca aaacttggtt aaacaagctc aattgactgc cgctactaac 1680
gctaaacaat aa 1692
<210> 11
<211> 563
<212> PRT
<213> Saccharomyces cerevisiae
<400> 11
Met Ser Glu Ile Thr Leu Gly Lys Tyr Leu Phe Glu Arg Leu Lys Gln
1 5 10 15
Val Asn Val Asn Thr Ile Phe Gly Leu Pro Gly Asp Phe Asn Leu Ser
20 25 30
Leu Leu Asp Lys Ile Tyr Glu Val Asp Gly Leu Arg Trp Ala Gly Asn
35 40 45
Ala Asn Glu Leu Asn Ala Ala Tyr Ala Ala Asp Gly Tyr Ala Arg Ile
50 55 60
Lys Gly Leu Ser Val Leu Val Thr Thr Phe Gly Val Gly Glu Leu Ser
65 70 75 80
Ala Leu Asn Gly Ile Ala Gly Ser Tyr Ala Glu His Val Gly Val Leu
85 90 95
His Val Val Gly Val Pro Ser Ile Ser Ala Gln Ala Lys Gln Leu Leu
100 105 110
Leu His His Thr Leu Gly Asn Gly Asp Phe Thr Val Phe His Arg Met
115 120 125
Ser Ala Asn Ile Ser Glu Thr Thr Ser Met Ile Thr Asp Ile Ala Thr
130 135 140
Ala Pro Ser Glu Ile Asp Arg Leu Ile Arg Thr Thr Phe Ile Thr Gln
145 150 155 160
Arg Pro Ser Tyr Leu Gly Leu Pro Ala Asn Leu Val Asp Leu Lys Val
165 170 175
Pro Gly Ser Leu Leu Glu Lys Pro Ile Asp Leu Ser Leu Lys Pro Asn
180 185 190
Asp Pro Glu Ala Glu Lys Glu Val Ile Asp Thr Val Leu Glu Leu Ile
195 200 205
Gln Asn Ser Lys Asn Pro Val Ile Leu Ser Asp Ala Cys Ala Ser Arg
210 215 220
His Asn Val Lys Lys Glu Thr Gln Lys Leu Ile Asp Leu Thr Gln Phe
225 230 235 240
Pro Ala Phe Val Thr Pro Leu Gly Lys Gly Ser Ile Asp Glu Gln His
245 250 255
Pro Arg Tyr Gly Gly Val Tyr Val Gly Thr Leu Ser Lys Gln Asp Val
260 265 270
Lys Gln Ala Val Glu Ser Ala Asp Leu Ile Leu Ser Val Gly Ala Leu
275 280 285
Leu Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Ser Tyr Lys Thr Lys
290 295 300
Asn Val Val Glu Phe His Ser Asp Tyr Val Lys Val Lys Asn Ala Thr
305 310 315 320
Phe Leu Gly Val Gln Met Lys Phe Ala Leu Gln Asn Leu Leu Lys Val
325 330 335
Ile Pro Asp Val Val Lys Gly Tyr Lys Ser Val Pro Val Pro Thr Lys
340 345 350
Thr Pro Ala Asn Lys Gly Val Pro Ala Ser Thr Pro Leu Lys Gln Glu
355 360 365
Trp Leu Trp Asn Glu Leu Ser Lys Phe Leu Gln Glu Gly Asp Val Ile
370 375 380
Ile Ser Glu Thr Gly Thr Ser Ala Phe Gly Ile Asn Gln Thr Ile Phe
385 390 395 400
Pro Lys Asp Ala Tyr Gly Ile Ser Gln Val Leu Trp Gly Ser Ile Gly
405 410 415
Phe Thr Thr Gly Ala Thr Leu Gly Ala Ala Phe Ala Ala Glu Glu Ile
420 425 430
Asp Pro Asn Lys Arg Val Ile Leu Phe Ile Gly Asp Gly Ser Leu Gln
435 440 445
Leu Thr Val Gln Glu Ile Ser Thr Met Ile Arg Trp Gly Leu Lys Pro
450 455 460
Tyr Leu Phe Val Leu Asn Asn Asp Gly Tyr Thr Ile Glu Lys Leu Ile
465 470 475 480
His Gly Pro His Ala Glu Tyr Asn Glu Ile Gln Thr Trp Asp His Leu
485 490 495
Ala Leu Leu Pro Ala Phe Gly Ala Lys Lys Tyr Glu Asn His Lys Ile
500 505 510
Ala Thr Thr Gly Glu Trp Asp Ala Leu Thr Thr Asp Ser Glu Phe Gln
515 520 525
Lys Asn Ser Val Ile Arg Leu Ile Glu Leu Lys Leu Pro Val Phe Asp
530 535 540
Ala Pro Glu Ser Leu Ile Lys Gln Ala Gln Leu Thr Ala Ala Thr Asn
545 550 555 560
Ala Lys Gln
<210> 12
<211> 1692
<212> DNA
<213> Saccharomyces cerevisiae
<400> 12
atgtctgaaa ttactcttgg aaaatactta tttgaaagat tgaagcaagt taatgttaac 60
accatttttg ggctaccagg cgacttcaac ttgtccctat tggacaagat ttacgaggta 120
gatggattga gatgggctgg taatgcaaat gagctgaacg ccgcctatgc cgccgatggt 180
tacgcacgca tcaagggttt atctgtgctg gtaactactt ttggcgtagg tgaattatcc 240
gccttgaatg gtattgcagg atcgtatgca gaacacgtcg gtgtactgca tgttgttggt 300
gtcccctcta tctccgctca ggctaagcaa ttgttgttgc atcatacctt gggtaacggt 360
gattttaccg tttttcacag aatgtccgcc aatatctcag aaactacatc aatgattaca 420
gacattgcta cagccccttc agaaatcgat aggttgatca ggacaacatt tataacacaa 480
aggcctagct acttggggtt gccagcgaat ttggtagatc taaaggttcc tggttctctt 540
ttggaaaaac cgattgatct atcattaaaa cctaacgatc ccgaagctga aaaggaagtt 600
attgataccg tactagaatt gatccagaat tcgaaaaacc ctgttatact atcggatgcc 660
tgtgcttcta ggcacaacgt taaaaaagaa acccagaagt taattgattt gacgcaattc 720
ccagcttttg tgacacctct aggtaaaggg tcaatagatg aacagcatcc cagatatggc 780
ggtgtttatg tgggaacgct gtccaaacaa gacgtgaaac aggccgttga gtcggctgat 840
ttgatccttt cggtcggtgc tttgctctct gattttaaca caggttcgtt ttcctactcc 900
tacaagacta aaaatgtagt ggagtttcat tccgattacg taaaggtgaa gaacgctacg 960
ttcctcggtg tacaaatgaa atttgcacta caaaacttac tgaaggttat tcccgatgtt 1020
gttaagggct acaagagcgt tcccgtacca accaaaactc ccgcaaacaa aggtgtacct 1080
gctagcacgc ccttgaaaca agagtggttg tggaacgaat tgtccaaatt cttgcaagaa 1140
ggtgatgtta tcatttccga gaccggcacg tctgccttcg gtatcaatca aactatcttt 1200
cctaaggacg cctacggtat ctcgcaggtg ttgtgggggt ccatcggttt tacaacagga 1260
gcaactttag gtgctgcctt tgccgctgag gagattgacc ccaacaagag agtcatctta 1320
ttcataggtg acgggtcttt gcagttaacc gtccaagaaa tctccaccat gatcagatgg 1380
gggttaaagc cgtatctttt tgtccttaac aacgacggct acactatcga aaagctgatt 1440
catgggcctc acgcagagta caacgaaatc cagacctggg atcacctcgc cctgttgccc 1500
gcatttggtg cgaaaaagta cgaaaatcac aagatcgcca ctacgggtga gtgggatgcc 1560
ttaaccactg attcagagtt ccagaaaaac tcggtgatca gactaattga actgaaactg 1620
cccgtctttg atgctccgga aagtttgatc aaacaagcgc aattgactgc cgctacaaat 1680
gccaaacaat aa 1692
<210> 13
<211> 408
<212> PRT
<213> Methanosarcina thermophila
<400> 13
Met Lys Val Leu Val Ile Asn Ala Gly Ser Ser Ser Leu Lys Tyr Gln
1 5 10 15
Leu Ile Asp Met Thr Asn Glu Ser Ala Leu Ala Val Gly Leu Cys Glu
20 25 30
Arg Ile Gly Ile Asp Asn Ser Ile Ile Thr Gln Lys Lys Phe Asp Gly
35 40 45
Lys Lys Leu Glu Lys Leu Thr Asp Leu Pro Thr His Lys Asp Ala Leu
50 55 60
Glu Glu Val Val Lys Ala Leu Thr Asp Asp Glu Phe Gly Val Ile Lys
65 70 75 80
Asp Met Gly Glu Ile Asn Ala Val Gly His Arg Val Val His Gly Gly
85 90 95
Glu Lys Phe Thr Thr Ser Ala Leu Tyr Asp Glu Gly Val Glu Lys Ala
100 105 110
Ile Lys Asp Cys Phe Glu Leu Ala Pro Leu His Asn Pro Pro Asn Met
115 120 125
Met Gly Ile Ser Ala Cys Ala Glu Ile Met Pro Gly Thr Pro Met Val
130 135 140
Ile Val Phe Asp Thr Ala Phe His Gln Thr Met Pro Pro Tyr Ala Tyr
145 150 155 160
Met Tyr Ala Leu Pro Tyr Asp Leu Tyr Glu Lys His Gly Val Arg Lys
165 170 175
Tyr Gly Phe His Gly Thr Ser His Lys Tyr Val Ala Glu Arg Ala Ala
180 185 190
Leu Met Leu Gly Lys Pro Ala Glu Glu Thr Lys Ile Ile Thr Cys His
195 200 205
Leu Gly Asn Gly Ser Ser Ile Thr Ala Val Glu Gly Gly Lys Ser Val
210 215 220
Glu Thr Ser Met Gly Phe Thr Pro Leu Glu Gly Leu Ala Met Gly Thr
225 230 235 240
Arg Cys Gly Ser Ile Asp Pro Ala Ile Val Pro Phe Leu Met Glu Lys
245 250 255
Glu Gly Leu Thr Thr Arg Glu Ile Asp Thr Leu Met Asn Lys Lys Ser
260 265 270
Gly Val Leu Gly Val Ser Gly Leu Ser Asn Asp Phe Arg Asp Leu Asp
275 280 285
Glu Ala Ala Ser Lys Gly Asn Arg Lys Ala Glu Leu Ala Leu Glu Ile
290 295 300
Phe Ala Tyr Lys Val Lys Lys Phe Ile Gly Glu Tyr Ser Ala Val Leu
305 310 315 320
Asn Gly Ala Asp Ala Val Val Phe Thr Ala Gly Ile Gly Glu Asn Ser
325 330 335
Ala Ser Ile Arg Lys Arg Ile Leu Thr Gly Leu Asp Gly Ile Gly Ile
340 345 350
Lys Ile Asp Asp Glu Lys Asn Lys Ile Arg Gly Gln Glu Ile Asp Ile
355 360 365
Ser Thr Pro Asp Ala Lys Val Arg Val Phe Val Ile Pro Thr Asn Glu
370 375 380
Glu Leu Ala Ile Ala Arg Glu Thr Lys Glu Ile Val Glu Thr Glu Val
385 390 395 400
Lys Leu Arg Ser Ser Ile Pro Val
405
<210> 14
<211> 1227
<212> DNA
<213> Methanosarcina thermophila
<400> 14
atgaaagtac tggttataaa cgcagggagt tcatctctca aatatcaatt aattgatatg 60
acaaatgagt cagctcttgc agtaggtctt tgcgagagga taggtattga caactcgatc 120
atcactcaga agaagtttga cggcaagaag ctggaaaagc tgactgacct ccccactcac 180
aaggacgcac ttgaggaagt cgtaaaagct cttaccgatg atgaattcgg tgtcatcaaa 240
gacatgggtg agatcaatgc agtcggacac agagttgtgc atggtggaga gaaattcacg 300
acgtctgctt tatatgatga gggcgtagaa aaggctatca aggactgctt tgaactggca 360
ccccttcaca accctccaaa catgatggga atttcagctt gtgcagagat catgcctggg 420
acgccaatgg ttattgtttt tgatactgca ttccaccaga caatgccgcc atatgcctac 480
atgtatgctc tcccgtacga cctgtacgag aagcatgggg tcaggaaata cggtttccac 540
ggcacatccc acaagtacgt tgccgaaagg gctgctctta tgctcggaaa gcccgcagaa 600
gaaaccaaaa ttatcacctg tcaccttgga aatggttcaa gcattacagc tgtagaaggc 660
ggaaaatccg ttgaaaccag catgggcttc acacctcttg aagggcttgc aatgggcaca 720
agatgcggtt cgattgaccc tgcaatagtc cccttcctta tggaaaaaga aggcttgaca 780
acaagagaaa ttgacaccct tatgaacaag aagtcaggtg tgcttggtgt ttccgggctc 840
agcaatgact tcagagacct cgatgaagca gcttccaagg gcaacaggaa agccgaactt 900
gctcttgaaa ttttcgcata caaggtcaag aagttcatag gtgaatattc agctgtcctc 960
aatggtgcag atgcagtggt ctttactgca ggcattggag aaaacagcgc aagcatcagg 1020
aagagaatcc tcaccggtct tgatggcatc ggcataaaaa tcgatgacga aaagaacaag 1080
atcagaggtc aggaaatcga tatcagcaca ccggatgcaa aagtaagagt ttttgtcatc 1140
ccaaccaatg aggaacttgc cattgcaagg gaaacaaagg aaattgttga gaccgaagtg 1200
aagttacgca gttctatacc tgtataa 1227
<210> 15
<211> 400
<212> PRT
<213> Escherichia coli
<400> 15
Met Ser Ser Lys Leu Val Leu Val Leu Asn Cys Gly Ser Ser Ser Leu
1 5 10 15
Lys Phe Ala Ile Ile Asp Ala Val Asn Gly Glu Glu Tyr Leu Ser Gly
20 25 30
Leu Ala Glu Cys Phe His Leu Pro Glu Ala Arg Ile Lys Trp Lys Met
35 40 45
Asp Gly Asn Lys Gln Glu Ala Ala Leu Gly Ala Gly Ala Ala His Ser
50 55 60
Glu Ala Leu Asn Phe Ile Val Asn Thr Ile Leu Ala Gln Lys Pro Glu
65 70 75 80
Leu Ser Ala Gln Leu Thr Ala Ile Gly His Arg Ile Val His Gly Gly
85 90 95
Glu Lys Tyr Thr Ser Ser Val Val Ile Asp Glu Ser Val Ile Gln Gly
100 105 110
Ile Lys Asp Ala Ala Ser Phe Ala Pro Leu His Asn Pro Ala His Leu
115 120 125
Ile Gly Ile Glu Glu Ala Leu Lys Ser Phe Pro Gln Leu Lys Asp Lys
130 135 140
Asn Val Ala Val Phe Asp Thr Ala Phe His Gln Thr Met Pro Glu Glu
145 150 155 160
Ser Tyr Leu Tyr Ala Leu Pro Tyr Asn Leu Tyr Lys Glu His Gly Ile
165 170 175
Arg Arg Tyr Gly Ala His Gly Thr Ser His Phe Tyr Val Thr Gln Glu
180 185 190
Ala Ala Lys Met Leu Asn Lys Pro Val Glu Glu Leu Asn Ile Ile Thr
195 200 205
Cys His Leu Gly Asn Gly Gly Ser Val Ser Ala Ile Arg Asn Gly Lys
210 215 220
Cys Val Asp Thr Ser Met Gly Leu Thr Pro Leu Glu Gly Leu Val Met
225 230 235 240
Gly Thr Arg Ser Gly Asp Ile Asp Pro Ala Ile Ile Phe His Leu His
245 250 255
Asp Thr Leu Gly Met Ser Val Asp Ala Ile Asn Lys Leu Leu Thr Lys
260 265 270
Glu Ser Gly Leu Leu Gly Leu Thr Glu Val Thr Ser Asp Cys Arg Tyr
275 280 285
Val Glu Asp Asn Tyr Ala Thr Lys Glu Asp Ala Lys Arg Ala Met Asp
290 295 300
Val Tyr Cys His Arg Leu Ala Lys Tyr Ile Gly Ala Tyr Thr Ala Leu
305 310 315 320
Met Asp Gly Arg Leu Asp Ala Val Val Phe Thr Gly Gly Ile Gly Glu
325 330 335
Asn Ala Ala Met Val Arg Glu Leu Ser Leu Gly Lys Leu Gly Val Leu
340 345 350
Gly Phe Glu Val Asp His Glu Arg Asn Leu Ala Ala Arg Phe Gly Lys
355 360 365
Ser Gly Phe Ile Asn Lys Glu Gly Thr Arg Pro Ala Val Val Ile Pro
370 375 380
Thr Asn Glu Glu Leu Val Ile Ala Gln Asp Ala Ser Arg Leu Thr Ala
385 390 395 400
<210> 16
<211> 1203
<212> DNA
<213> Escherichia coli
<400> 16
atgtcgagta agttagtact ggttctgaac tgcggtagtt cttcactgaa atttgccatc 60
atcgatgcag taaatggtga agagtacctt tctggtttag ccgaatgttt ccacctgccc 120
gaagcacgta tcaaatggaa aatggacggc aataaacagg aagcggcttt aggtgcaggc 180
gccgctcaca gcgaagcgct caactttatc gttaatacta ttctggcaca aaaaccagaa 240
ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac acggcggcga aaagtatacc 300
agctccgtag tgatcgatga gtctgttatt cagggtatca aagatgcagc ttcttttgca 360
ccgctgcaca acccggctca cctgatcggt atcgaagaag ctctgaaatc tttcccacag 420
ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc accagactat gccggaagag 480
tcttacctct acgccctgcc ttacaacctg tacaaagagc acggcatccg tcgttacggc 540
gcgcacggca ccagccactt ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg 600
gtagaagaac tgaacatcat cacctgccac ctgggcaacg gtggttccgt ttctgctatc 660
cgcaacggta aatgcgttga cacctctatg ggcctgaccc cgctggaagg tctggtcatg 720
ggtacccgtt ctggtgatat cgatccggcg atcatcttcc acctgcacga caccctgggc 780
atgagcgttg acgcaatcaa caaactgctg accaaagagt ctggcctgct gggtctgacc 840
gaagtgacca gcgactgccg ctatgttgaa gacaactacg cgacgaaaga agacgcgaag 900
cgcgcaatgg acgtttactg ccaccgcctg gcgaaataca tcggtgccta cactgcgctg 960
atggatggtc gtctggacgc tgttgtattc actggtggta tcggtgaaaa tgccgcaatg 1020
gttcgtgaac tgtctctggg caaactgggc gtgctgggct ttgaagttga tcatgaacgc 1080
aacctggctg cacgtttcgg caaatctggt ttcatcaaca aagaaggtac ccgtcctgcg 1140
gtggttatcc caaccaacga agaactggtt atcgcgcaag acgcgagccg cctgactgcc 1200
taa 1203
<210> 17
<211> 501
<212> PRT
<213> Saccharomyces cerevisiae
<400> 17
Met Thr Lys Leu His Phe Asp Thr Ala Glu Pro Val Lys Ile Thr Leu
1 5 10 15
Pro Asn Gly Leu Thr Tyr Glu Gln Pro Thr Gly Leu Phe Ile Asn Asn
20 25 30
Lys Phe Met Lys Ala Gln Asp Gly Lys Thr Tyr Pro Val Glu Asp Pro
35 40 45
Ser Thr Glu Asn Thr Val Cys Glu Val Ser Ser Ala Thr Thr Glu Asp
50 55 60
Val Glu Tyr Ala Ile Glu Cys Ala Asp Arg Ala Phe His Asp Thr Glu
65 70 75 80
Trp Ala Thr Gln Asp Pro Arg Glu Arg Gly Arg Leu Leu Ser Lys Leu
85 90 95
Ala Asp Glu Leu Glu Ser Gln Ile Asp Leu Val Ser Ser Ile Glu Ala
100 105 110
Leu Asp Asn Gly Lys Thr Leu Ala Phe Lys Ala Arg Gly Asp Val Thr
115 120 125
Ile Ala Ile Asn Cys Leu Arg Asp Ala Ala Ala Tyr Ala Asp Lys Val
130 135 140
Asn Gly Arg Thr Ile Asn Thr Gly Asp Gly Tyr Met Asn Phe Thr Thr
145 150 155 160
Leu Glu Pro Ile Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro
165 170 175
Ile Met Met Leu Ala Trp Lys Ile Ala Pro Ala Leu Ala Met Gly Asn
180 185 190
Val Cys Ile Leu Lys Pro Ala Ala Val Thr Pro Leu Asn Ala Leu Tyr
195 200 205
Phe Ala Ser Leu Cys Lys Lys Val Gly Ile Pro Ala Gly Val Val Asn
210 215 220
Ile Val Pro Gly Pro Gly Arg Thr Val Gly Ala Ala Leu Thr Asn Asp
225 230 235 240
Pro Arg Ile Arg Lys Leu Ala Phe Thr Gly Ser Thr Glu Val Gly Lys
245 250 255
Ser Val Ala Val Asp Ser Ser Glu Ser Asn Leu Lys Lys Ile Thr Leu
260 265 270
Glu Leu Gly Gly Lys Ser Ala His Leu Val Phe Asp Asp Ala Asn Ile
275 280 285
Lys Lys Thr Leu Pro Asn Leu Val Asn Gly Ile Phe Lys Asn Ala Gly
290 295 300
Gln Ile Cys Ser Ser Gly Ser Arg Ile Tyr Val Gln Glu Gly Ile Tyr
305 310 315 320
Asp Glu Leu Leu Ala Ala Phe Lys Ala Tyr Leu Glu Thr Glu Ile Lys
325 330 335
Val Gly Asn Pro Phe Asp Lys Ala Asn Phe Gln Gly Ala Ile Thr Asn
340 345 350
Arg Gln Gln Phe Asp Thr Ile Met Asn Tyr Ile Asp Ile Gly Lys Lys
355 360 365
Glu Gly Ala Lys Ile Leu Thr Gly Gly Glu Lys Val Gly Asp Lys Gly
370 375 380
Tyr Phe Ile Arg Pro Thr Val Phe Tyr Asp Val Asn Glu Asp Met Arg
385 390 395 400
Ile Val Lys Glu Glu Ile Phe Gly Pro Val Val Thr Val Ala Lys Phe
405 410 415
Lys Thr Leu Glu Glu Gly Val Glu Met Ala Asn Ser Ser Glu Phe Gly
420 425 430
Leu Gly Ser Gly Ile Glu Thr Glu Ser Leu Ser Thr Gly Leu Lys Val
435 440 445
Ala Lys Met Leu Lys Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asp
450 455 460
Phe Asp Ser Arg Val Pro Phe Gly Gly Val Lys Gln Ser Gly Tyr Gly
465 470 475 480
Arg Glu Met Gly Glu Glu Val Tyr His Ala Tyr Thr Glu Val Lys Ala
485 490 495
Val Arg Ile Lys Leu
500
<210> 18
<211> 1506
<212> DNA
<213> Saccharomyces cerevisiae
<400> 18
atgactaagc tacactttga cactgctgaa ccagtcaaga tcacacttcc aaatggtttg 60
acatacgagc aaccaaccgg tctattcatt aacaacaagt ttatgaaagc tcaagacggt 120
aagacctatc ccgtcgaaga tccttccact gaaaacaccg tttgtgaggt ctcttctgcc 180
accactgaag atgttgaata tgctatcgaa tgtgccgacc gtgctttcca cgacactgaa 240
tgggctaccc aagacccaag agaaagaggc cgtctactaa gtaagttggc tgacgaattg 300
gaaagccaaa ttgacttggt ttcttccatt gaagctttgg acaatggtaa aactttggcc 360
tttaaggccc gtggggatgt taccattgca atcaactgtc taagagatgc tgctgcctat 420
gccgacaaag tcaacggtag aacaatcaac accggtgacg gctacatgaa cttcaccacc 480
ttagagccaa tcggtgtctg tggtcaaatt attccatgga actttccaat aatgatgttg 540
gcttggaaga tcgccccagc attggccatg ggtaacgtct gtatcttgaa acccgctgct 600
gtcacacctt taaatgccct atactttgct tctttatgta agaaggttgg tattccagct 660
ggtgtcgtca acatcgttcc aggtcctggt agaactgttg gtgctgcttt gaccaacgac 720
ccaagaatca gaaagctggc ttttaccggt tctacagaag tcggtaagag tgttgctgtc 780
gactcttctg aatctaactt gaagaaaatc actttggaac taggtggtaa gtccgcccat 840
ttggtctttg acgatgctaa cattaagaag actttaccaa atctagtaaa cggtattttc 900
aagaacgctg gtcaaatttg ttcctctggt tctagaattt acgttcaaga aggtatttac 960
gacgaactat tggctgcttt caaggcttac ttggaaaccg aaatcaaagt tggtaatcca 1020
tttgacaagg ctaacttcca aggtgctatc actaaccgtc aacaattcga cacaattatg 1080
aactacatcg atatcggtaa gaaagaaggc gccaagatct taactggtgg cgaaaaagtt 1140
ggtgacaagg gttacttcat cagaccaacc gttttctacg atgttaatga agacatgaga 1200
attgttaagg aagaaatttt tggaccagtt gtcactgtcg caaagttcaa gactttagaa 1260
gaaggtgtcg aaatggctaa cagctctgaa ttcggtctag gttctggtat cgaaacagaa 1320
tctttgagca caggtttgaa ggtggccaag atgttgaagg ccggtaccgt ctggatcaac 1380
acatacaacg attttgactc cagagttcca ttcggtggtg ttaagcaatc tggttacggt 1440
agagaaatgg gtgaagaagt ctaccatgca tacactgaag taaaagctgt cagaattaag 1500
ttgtaa 1506
<210> 19
<211> 314
<212> PRT
<213> Saccharomyces cerevisiae
<400> 19
Met Pro Ser Thr Thr Asn Thr Ala Ala Ala Asn Val Ile Glu Lys Lys
1 5 10 15
Pro Val Ser Phe Ser Asn Ile Leu Leu Gly Ala Cys Leu Asn Leu Ser
20 25 30
Glu Val Thr Thr Leu Gly Gln Pro Leu Glu Val Val Lys Thr Thr Met
35 40 45
Ala Ala Asn Arg Asn Phe Thr Phe Leu Glu Ser Val Lys His Val Trp
50 55 60
Ser Arg Gly Gly Ile Leu Gly Tyr Tyr Gln Gly Leu Ile Pro Trp Ala
65 70 75 80
Trp Ile Glu Ala Ser Thr Lys Gly Ala Val Leu Leu Phe Val Ser Ala
85 90 95
Glu Ala Glu Tyr Arg Phe Lys Ser Leu Gly Leu Asn Asn Phe Ala Ser
100 105 110
Gly Ile Leu Gly Gly Val Thr Gly Gly Val Thr Gln Ala Tyr Leu Thr
115 120 125
Met Gly Phe Cys Thr Cys Met Lys Thr Val Glu Ile Thr Arg His Lys
130 135 140
Ser Ala Ser Ala Gly Gly Val Pro Gln Ser Ser Trp Ser Val Phe Lys
145 150 155 160
Asn Ile Tyr Lys Lys Glu Gly Ile Arg Gly Ile Asn Lys Gly Val Asn
165 170 175
Ala Val Ala Ile Arg Gln Met Thr Asn Trp Gly Ser Arg Phe Gly Leu
180 185 190
Ser Arg Leu Val Glu Asp Gly Ile Arg Lys Ile Thr Gly Lys Thr Asn
195 200 205
Lys Asp Asp Lys Leu Asn Pro Phe Glu Lys Ile Gly Ala Ser Ala Leu
210 215 220
Gly Gly Gly Leu Ser Ala Trp Asn Gln Pro Ile Glu Val Ile Arg Val
225 230 235 240
Glu Met Gln Ser Lys Lys Glu Asp Pro Asn Arg Pro Lys Asn Leu Thr
245 250 255
Val Gly Lys Thr Phe Lys Tyr Ile Tyr Gln Ser Asn Gly Leu Lys Gly
260 265 270
Leu Tyr Arg Gly Val Thr Pro Arg Ile Gly Leu Gly Ile Trp Gln Thr
275 280 285
Val Phe Met Val Gly Phe Gly Asp Met Ala Lys Glu Phe Val Ala Arg
290 295 300
Met Thr Gly Glu Thr Pro Val Ala Lys His
305 310
<210> 20
<211> 945
<212> DNA
<213> Saccharomyces cerevisiae
<400> 20
atgccatcta ccactaatac tgctgcagca aacgtaatag aaaaaaagcc agtctcgttt 60
tctaatatcc tattgggtgc ctgtttaaac ttgtcagagg tgactacatt agggcaacct 120
ttggaggttg ttaagaccac aatggctgca aacagaaact tcacattttt agaatctgtt 180
aagcatgtct ggtcaagagg tggtatcttg ggttactacc aaggtttgat tccatgggca 240
tggatcgaag cctccactaa aggtgctgtg ttgctgttcg tgtcagctga ggctgagtat 300
cgtttcaaaa gtttggggtt gaacaacttt gcctcaggta tattaggtgg tgtcacgggt 360
ggtgtcactc aagcctactt aaccatgggg ttctgtacct gtatgaaaac ggtggaaatt 420
acaagacata aatctgcctc cgcaggtggt gtcccacaat cttcttggag tgtgttcaag 480
aatatttata aaaaggaagg tattagaggt attaataagg gtgttaatgc tgttgctatt 540
agacaaatga ccaactgggg ttctcgtttt ggtttgtcca gactagtgga agatggtatc 600
agaaagatca ccgggaaaac caataaagac gacaagttga atccgttcga gaaaattggt 660
gccagtgctt taggtggtgg tttaagtgct tggaatcaac caatcgaagt cattagagtt 720
gaaatgcaat ctaagaagga agatccaaac agaccaaaaa atttgactgt tggtaagaca 780
tttaaataca tctatcaatc aaatggtcta aagggtcttt accgtggtgt caccccaaga 840
attggtttag gtatctggca aactgtcttc atggttggtt ttggtgatat ggcgaaggaa 900
tttgtcgcca gaatgactgg tgaaacccca gttgccaaac attag 945
<210> 21
<211> 865
<212> PRT
<213> Rhodosporidium toruloides
<400> 21
Met Ala Asp Asn Ala Asp Ala Pro Pro Pro Pro Ile Val Pro Ser Gln
1 5 10 15
Tyr Ala Gln His Pro Asp Ala Pro Leu Ser Ser Leu Pro Val Gln Leu
20 25 30
Asp Pro Ser Gln Tyr Thr Ala Lys Tyr Pro Ala Lys His Leu Asp Ala
35 40 45
Ile Val Ala Asn Trp Arg Leu Ser Cys Tyr Leu Gly Ala Ser Gln Ile
50 55 60
Phe Leu Gln Ser Asn Ala Ile Leu Ser Arg Lys Leu Thr Lys Asp Asp
65 70 75 80
Val Lys Pro Arg Arg Ala His Thr Asn Leu Ala Gly Asp Ile Gln Gly
85 90 95
Gly Leu Ser Leu Ala Tyr Val His Thr Gln Ala Leu Ile Arg Arg Lys
100 105 110
Gly Asp Glu Glu Gly Ala Glu Pro Lys Met Ile Phe Val Thr Gly Pro
115 120 125
Gly His Gly Ala Pro Ala Ile Leu Ser Pro Leu Tyr Ile Glu Gly Ala
130 135 140
Ile Ser Lys Phe Tyr Pro Gln Tyr Pro Leu Asn Glu Gln Gly Leu Glu
145 150 155 160
Lys Phe Val Lys Tyr Phe Ser Trp Pro Gly Gly Phe Pro Ser His Val
165 170 175
Asn Ala Glu Thr Pro Gly Cys Ile His Glu Gly Gly Glu Leu Gly Tyr
180 185 190
Ala Leu Gly Val Ala Tyr Gly Ser Val Met Asp Arg Pro Glu Gln Ile
195 200 205
Ser Val Val Val Val Gly Asp Gly Glu Ser Glu Thr Gly Pro Thr Ala
210 215 220
Thr Ala Trp His Ser His Lys Trp Leu Asp Pro Ala Glu Ser Gly Ala
225 230 235 240
Val Leu Pro Ile Leu His Val Asn Gly Phe Lys Ile Ser Glu Arg Thr
245 250 255
Ile Pro Gly Thr Met Asp Asn Val Glu Leu Ser Leu Leu Tyr Ser Gly
260 265 270
Tyr Gly Tyr Gln Val Arg Phe Val Glu Tyr Lys Ala Gln Gly Glu Ala
275 280 285
His Met Gly Gly Asn Asp Pro Ala Asp Arg Val Leu His Glu Asp Met
290 295 300
Ala Ala Ser Leu Asp Trp Ala Tyr Gly Glu Ile Arg Lys Ile Gln Lys
305 310 315 320
Ala Ala Arg Ser Gly Gly Lys Pro Ile Asp Lys Pro Arg Trp Pro Met
325 330 335
Ile Ile Leu Arg Ser Pro Lys Gly Trp Thr Gly Pro Ser Ser Glu His
340 345 350
Gly Lys Gln Leu Leu Asn Asn Phe Ala Ser His Gln Val Pro Leu Pro
355 360 365
Asp Ala Lys Thr Asp Asp Glu Ala Asn Ala Tyr Leu Glu Arg Trp Leu
370 375 380
Lys Ser Tyr Glu Ala Asp Lys Leu Phe Asp Phe Ser Glu Asp Asn Leu
385 390 395 400
Lys Arg Gly Thr Ile Phe Asp Gln Leu Leu Tyr Glu Ala Leu Pro Lys
405 410 415
Asp Met Glu Arg Arg Leu Gly Phe Val Lys Glu Thr Tyr Asn Gly Tyr
420 425 430
Lys Pro Leu Glu Leu Asp Asp Trp Lys Lys Tyr Gly Phe Lys Lys Gly
435 440 445
Glu Asp Val Ser Cys Met Lys Ala Ile Ala Gly Tyr Leu Thr Asp Val
450 455 460
Ile Lys Arg Asn Pro Lys Glu Phe Arg Ile Phe Ser Pro Asp Glu Leu
465 470 475 480
Ala Leu Asn Lys Leu Asp Gly Val Phe Ser Val Thr Glu Arg Asn Met
485 490 495
Gln Trp Asp Pro Glu Thr Ala His Lys Gly Gly Arg Val Thr Glu Met
500 505 510
Leu Ser Glu His Ser Leu Gln Ala Trp Leu Gln Gly Tyr Thr Leu Thr
515 520 525
Gly Arg His Gly Val Phe Pro Ser Tyr Glu Ala Phe Leu Gly Ile Val
530 535 540
Ala Thr Met Thr Val Gln Tyr Thr Lys Phe Met Lys Met Ala Leu Glu
545 550 555 560
Thr Asn Trp Arg Gly Pro Thr Ala Ser Leu Thr Tyr Ile Glu Thr Ser
565 570 575
Thr Trp Thr Arg Gln Glu His Asn Gly Tyr Ser His Gln Asn Pro Gly
580 585 590
Phe Val Ser Thr Val Leu Ser Leu Pro Ser Gln Leu Ala Arg Val Tyr
595 600 605
Phe Pro Ser Asp Ala Asn Thr Ser Val Ser Val Ile Ala His Cys Leu
610 615 620
Arg Ser Lys Asn Tyr Ile Asn Leu Ile Val Gly Thr Lys Ala Pro Thr
625 630 635 640
Pro Val Tyr Leu Ser Val Glu Glu Ala Glu Arg His Cys Ile Ala Gly
645 650 655
Ala Ser Val Trp Glu Asn Tyr Ser Val Asp Lys Gly Val Asp Pro Asp
660 665 670
Val Val Leu Val Gly Ile Gly Tyr Glu Leu Thr Glu Glu Val Ile His
675 680 685
Ala Ala Ala Leu Leu Arg Lys Asp Phe Gly Thr Glu Leu Arg Val Arg
690 695 700
Val Val Asn Val Val Asp Leu Leu Val Leu Ala Pro Lys Gly Asp His
705 710 715 720
Pro His Ala Leu Asp Glu Ala Gly Phe Asn Ser Leu Phe Pro Pro Gly
725 730 735
Val Pro Ile Ile Phe Asn Tyr His Gly Tyr Ala Gly Gln Leu Ala Ser
740 745 750
Leu Leu Phe Asp Arg Lys His Ser Val Gly Arg Ser Arg Met Arg Ile
755 760 765
Phe Ala Tyr Ser Glu Gln Gly Thr Thr Thr Thr Pro Phe Ala Met Met
770 775 780
Cys Cys Asn Asn Thr Asp Arg Phe Asn Leu Ala Ala Glu Ala Leu Glu
785 790 795 800
Met Val Thr Leu Asn Leu Thr Thr Gln His Asn Ile Thr Gly Glu Glu
805 810 815
Lys Arg His Arg Val Gly Ser Val Val Ala Arg Ala His Glu Arg Met
820 825 830
Ser Phe Tyr Lys His Lys Lys Val Val Met Met Arg Tyr Ala Ala Glu
835 840 845
Thr Gln Lys Asp His Pro Glu Ile Gly Glu Val Ala Thr Leu Ala Glu
850 855 860
Gln
865
<210> 22
<211> 2598
<212> DNA
<213> Rhodosporidium toruloides
<400> 22
atggcagaca acgcagacgc ccctcccccg cccatcgtgc cctcgcagta cgcccagcac 60
ccggacgcac ctctctcgag tctccccgtc cagctcgacc cgagccagta caccgccaag 120
taccccgcca agcacctcga tgccattgtc gcaaactggc gcttgtcgtg ctacttggga 180
gcgtcgcaaa tcttcctcca gtcgaacgcg atcctctcga ggaagttgac caaggacgac 240
gtcaagcctc ggagggccca cactaacctt gctggcgaca tacagggagg tctttcgctc 300
gcctacgtcc acactcaagc gctcatccgc cgcaagggcg acgaggaagg cgccgaaccc 360
aagatgatct tcgtcaccgg tccgggccac ggcgcacccg ccatcctctc gcctctctac 420
atcgaaggcg ccatctccaa gttttacccc cagtaccctc tcaacgagca aggactcgag 480
aagtttgtca agtacttctc gtggccggga ggattccctt cgcatgttaa cgctgagacg 540
ccgggatgta tccacgaggg cggagagctc gggtatgcgc ttggagtcgc gtacggatcg 600
gtcatggacc gcccggagca gatctcggtc gttgttgtcg gtgacggcga gagcgagacg 660
ggaccgaccg cgacggcgtg gcactcgcac aagtggctcg accccgccga gtcaggcgcc 720
gtcctcccca tccttcacgt caacggcttc aagatctcgg agcgcaccat ccccggcacg 780
atggacaacg tcgagctttc ccttctctac tccggctacg ggtaccaggt ccgcttcgtc 840
gaatacaagg cgcagggcga ggcgcacatg ggtggcaacg atccggccga ccgcgtcctc 900
cacgaggata tggctgcctc gctcgactgg gcctacggcg agatccgcaa gatccagaag 960
gctgctcgct ccggcggcaa gcccatcgac aagcctcgct ggccgatgat catcctccgc 1020
tcgcccaagg gatggaccgg cccgtcgagt gagcacggca agcagctcct caacaacttc 1080
gcctcgcacc aggtccccct ccccgacgcc aagacggacg acgaagcgaa cgcctacctc 1140
gagcgctggc tcaagtcgta cgaagccgac aaactcttcg acttttccga agacaacctc 1200
aagcgcggta ccatctttga ccagctcttg tacgaggcgc tcccgaagga tatggaaagg 1260
cgccttgggt ttgtcaagga gacctacaac ggttacaagc cccttgagct ggacgactgg 1320
aagaagtacg gcttcaagaa gggcgaggat gtctcgtgca tgaaggcgat cgctggctac 1380
ctcaccgacg tgatcaagcg caacccgaaa gagttccgca tcttctcgcc cgacgagctc 1440
gccttgaaca agctcgatgg cgtcttctcc gtcaccgagc gcaacatgca gtgggacccg 1500
gagacggcgc acaagggcgg cagggtcacc gagatgcttt ctgagcactc gttgcaggcc 1560
tggttgcagg ggtacaccct cacgggcagg catggcgtct tcccctcgta cgaggccttc 1620
ctcggcatcg tcgcgaccat gaccgtccaa tacaccaagt tcatgaagat ggcgctcgag 1680
accaactggc gcggaccgac cgcctcgctg acctacatcg agacctcgac ctggacccgc 1740
caggagcaca acggctactc gcaccagaac cctggcttcg tctcgaccgt cctctcgctc 1800
ccctcccagc tcgcccgcgt ctacttcccc tcggacgcta acacgagcgt cagcgtcatc 1860
gcacactgct tgcgcagcaa gaactacatc aacctcatcg tcggcaccaa ggctcctacg 1920
cctgtctacc tcagcgtcga ggaggccgag cgtcactgca ttgccggcgc ttcggtctgg 1980
gagaactact cggtcgacaa gggtgtcgac ccggatgtcg tcctcgtcgg tatcggctac 2040
gagctgacgg aggaggtcat ccatgccgcg gcgctcctcc gcaaggactt tggcaccgaa 2100
ttgagggtca gggtcgtcaa tgtcgtcgac ttgctcgtcc tcgcgcccaa gggcgaccac 2160
ccgcatgctc tcgacgaagc cggcttcaac tcactcttcc cgcccggcgt tcctatcatc 2220
ttcaactacc acggctacgc tggccagctc gcgtcgctcc tcttcgaccg caagcactcg 2280
gtcggccgtt cgcgcatgcg catctttgcc tactcggagc agggcacgac gacgactccg 2340
ttcgccatga tgtgctgcaa caacaccgac aggttcaacc tcgcggccga ggcgctcgag 2400
atggtcaccc tcaacctaac gacgcagcac aacatcaccg gcgaggagaa gcgccaccgc 2460
gtcggctcgg tcgtcgcgcg cgcgcacgag cgcatgtcgt tctacaagca caagaaggtc 2520
gtcatgatgc gctacgctgc cgagactcag aaggaccacc cggagattgg cgaggttgcc 2580
acgcttgccg agcagtaa 2598
<210> 23
<211> 806
<212> PRT
<213> Aspergillus niger
<400> 23
Met Pro Gly Glu Val Ile Asp Arg Pro Asn Pro Lys Ala Glu Pro Ser
1 5 10 15
His Ile Pro Asp Leu Val Asn Gln Leu Gln Val Lys Leu Gln Glu Thr
20 25 30
Arg Leu Glu Glu Thr Asp Tyr Asn Ala Leu Leu Lys Phe Arg Arg Ala
35 40 45
Ala Ala Tyr Ile Ala Ala Ala Met Ile Phe Leu Gln Asp Asn Val Leu
50 55 60
Leu Lys Gln Asn Leu Arg His Glu Asp Ile Lys Pro Arg Leu Leu Gly
65 70 75 80
His Trp Gly Thr Cys Pro Gly Leu Ile Leu Val Tyr Ser His Leu Asn
85 90 95
Tyr Ile Ile Arg Lys Gln Asn Leu Asp Met Leu Tyr Val Val Gly Pro
100 105 110
Gly His Gly Ala Pro Ala Ile Leu Ala Ser Leu Trp Leu Glu Gly Ser
115 120 125
Leu Glu Lys Phe Tyr Pro His Tyr Ser Arg Asp Met Asp Gly Leu His
130 135 140
Glu Leu Ile Ser Thr Phe Ser Thr Ser Ala Gly Leu Pro Ser His Ile
145 150 155 160
Asn Ala Glu Thr Pro Gly Ala Ile His Glu Gly Gly Glu Leu Gly Tyr
165 170 175
Ala Leu Ala Val Ser Phe Gly Ala Val Met Asp Asn Pro Asp Met Ile
180 185 190
Val Thr Cys Val Val Gly Asp Gly Glu Ala Glu Thr Gly Pro Thr Ala
195 200 205
Thr Ser Trp His Ala Ile Lys Tyr Ile Asp Pro Ala Glu Ser Gly Ala
210 215 220
Val Leu Pro Ile Leu His Val Asn Gly Phe Lys Ile Ser Glu Arg Thr
225 230 235 240
Ile Tyr Gly Cys Met Asp Asn Lys Glu Leu Val Ser Leu Phe Thr Gly
245 250 255
Tyr Gly Tyr Gln Val Arg Ile Val Glu Asn Leu Asp Asp Ile Asp Ala
260 265 270
Asp Leu His Ser Ser Met Met Trp Ala Val Glu Glu Ile His Lys Ile
275 280 285
Gln Lys Ala Ala Arg Ser Gly Lys Pro Ile Met Lys Pro Arg Trp Pro
290 295 300
Met Ile Val Leu Arg Thr Pro Lys Gly Trp Ser Gly Pro Lys Glu Leu
305 310 315 320
His Gly Ser Phe Ile Glu Gly Ser Phe His Ser His Gln Val Pro Leu
325 330 335
Pro Asn Ala Lys Lys Asp Lys Glu Glu Leu Gln Ala Leu Gln Lys Trp
340 345 350
Leu Ser Ser Tyr Asn Pro His Glu Leu Phe Thr Glu Thr Gly Asp Ile
355 360 365
Ile Asp Asp Ile Lys Ser Val Ile Pro Leu Glu Asp Thr Lys Lys Leu
370 375 380
Gly Gln Arg Ala Glu Ala Tyr Lys Gly Tyr Arg Ala Pro Asp Leu Pro
385 390 395 400
Asp Trp Arg Lys Phe Gly Val Glu Lys Gly Ser Gln Gln Ser Ala Met
405 410 415
Lys Thr Ile Gly Lys Phe Ile Asp Gln Val Phe Thr Gln Asn Pro His
420 425 430
Gly Val Arg Val Phe Ser Pro Asp Glu Leu Glu Ser Asn Lys Leu Asp
435 440 445
Ala Ala Leu Ala His Thr Gly Arg Asn Phe Gln Trp Asp Gln Phe Ser
450 455 460
Asn Ala Lys Gly Gly Arg Val Ile Glu Val Leu Ser Glu His Leu Cys
465 470 475 480
Gln Gly Phe Met Gln Gly Tyr Thr Leu Thr Gly Arg Val Gly Ile Phe
485 490 495
Pro Ser Tyr Glu Ser Phe Leu Gly Ile Ile His Thr Met Met Val Gln
500 505 510
Tyr Ala Lys Phe Asn Lys Met Ala Gln Glu Thr Thr Trp His Lys Pro
515 520 525
Val Ser Ser Ile Asn Tyr Ile Glu Thr Ser Thr Trp Ala Arg Gln Glu
530 535 540
His Asn Gly Phe Ser His Gln Asn Pro Ser Phe Ile Gly Ala Val Leu
545 550 555 560
Arg Leu Lys Pro Thr Ala Ala Arg Val Tyr Leu Pro Pro Asp Ala Asn
565 570 575
Thr Phe Leu Thr Thr Leu His His Cys Leu Lys Ser Lys Asn Tyr Val
580 585 590
Asn Leu Met Val Gly Ser Lys Gln Pro Thr Pro Val Tyr Leu Ser Pro
595 600 605
Glu Glu Ala Glu Ser His Cys Arg Ala Gly Ala Ser Ile Trp Arg Phe
610 615 620
Cys Ser Thr Asp Asn Gly Leu Asn Pro Asp Val Val Leu Val Gly Ile
625 630 635 640
Gly Val Glu Val Met Phe Glu Val Ile Tyr Ala Ala Ala Ile Leu Arg
645 650 655
Lys Arg Cys Pro Glu Leu Arg Val Arg Val Val Asn Val Thr Asp Leu
660 665 670
Met Ile Leu Glu Lys Glu Gly Leu His Pro His Ala Leu Thr Thr Glu
675 680 685
Ala Phe Asp Ser Leu Phe Gly Ser Asp Arg Pro Ile His Phe Asn Tyr
690 695 700
His Gly Tyr Pro Gly Glu Leu Lys Gly Leu Leu Phe Gly Arg Pro Arg
705 710 715 720
Leu Asp Arg Val Ser Val Glu Gly Tyr Met Glu Glu Gly Ser Thr Thr
725 730 735
Thr Pro Phe Asp Met Met Leu Leu Asn Arg Val Ser Arg Tyr His Val
740 745 750
Ala Gln Ala Ala Val Ile Gly Ala Ser Arg Arg Asn Glu Lys Val Gln
755 760 765
Val Arg Gln His Glu Leu Val Ser Glu Phe Gly His Asn Ile Val Glu
770 775 780
Thr Arg Lys Tyr Ile Leu Ala Asn Arg Lys Asp Pro Asp Asp Thr Tyr
785 790 795 800
Asp Met Pro Ser Phe Glu
805
<210> 24
<211> 2421
<212> DNA
<213> Aspergillus niger
<400> 24
atgcctggag aggtcatcga caggccaaat cccaaggctg agccttcaca catccccgat 60
cttgtcaatc aattgcaggt caaacttcaa gagacgcgtt tggaggaaac tgattacaat 120
gcccttctga aattccgccg tgcagcggcc tacattgctg ctgcaatgat ctttctccaa 180
gacaatgtgc tgctgaagca gaatctaagg cacgaggaca tcaagcccag gcttcttggc 240
cactggggaa catgtcccgg gttgattctt gtatactctc acttgaacta catcatcaga 300
aagcagaacc tggatatgtt gtatgtcgtc gggcctggcc acggcgcgcc agctattttg 360
gcctcactgt ggcttgaggg ctctttagag aaattctacc cccactactc acgagacatg 420
gatggtctcc atgagctcat ctcgaccttc agcacaagtg ctggattacc aagccatatc 480
aatgcggaaa ctcccggtgc aatccatgaa ggtggtgaat tgggttatgc gttagctgtc 540
tcttttggtg ctgttatgga caatcccgac atgatcgtca cctgcgtggt tggtgacggg 600
gaagcagaaa ctggtcctac cgcgacgtcc tggcatgcaa tcaagtacat tgaccccgca 660
gaatcaggtg ccgtcctgcc gattctccac gttaatggct ttaagatcag cgagcgcacc 720
atttatggct gcatggacaa caaagagctg gtctccctct tcacgggtta tggataccag 780
gtgcgcattg ttgagaacct ggatgacatc gacgcagatc tccatagctc tatgatgtgg 840
gcagttgagg agatccacaa gatccaaaaa gcggcgcgtt ccggcaagcc aattatgaag 900
cctagatggc caatgattgt tttgcgcaca ccgaagggtt ggtcaggacc taaagagctc 960
cacgggtcat tcatagaggg atctttccac tcacatcagg ttcctctacc taatgcaaag 1020
aaggataaag aggagcttca ggctctgcag aaatggctgt cctcgtataa tccgcacgaa 1080
cttttcactg agacgggaga catcattgac gacatcaagt cagtgatccc tctggaagac 1140
accaagaagc ttgggcagcg agcagaagcc tacaagggct atagggcacc cgatctccca 1200
gactggcgca agtttggcgt agaaaagggc tcccagcaga gcgctatgaa aacaattgga 1260
aagttcattg accaagtgtt tacccaaaat cctcatggcg tccgtgtatt ttcgccagac 1320
gagctagaga gcaacaagct ggatgcagca ctggcgcaca cgggaaggaa ctttcagtgg 1380
gatcaattct cgaatgccaa aggcggccgc gtcatcgagg tgctcagtga gcacctgtgc 1440
cagggcttta tgcagggata cacgttgacg ggccgggtgg gcattttccc atcgtacgaa 1500
agcttcttgg gaatcatcca taccatgatg gtgcaatatg ccaaatttaa caaaatggct 1560
caagagacga cctggcataa gccggttagt agcatcaact atatcgaaac gagtacgtgg 1620
gctcgtcagg agcacaatgg attctctcac cagaacccct cctttatcgg agctgtgctc 1680
aggctgaagc ccaccgccgc gcgagtttat ctgccacctg atgctaacac atttttgacc 1740
acccttcacc actgtctcaa gtccaagaat tatgtcaacc tcatggtagg ttcaaaacag 1800
ccaactcccg tgtacttgag ccccgaggaa gcagagagcc actgccgagc cggcgcatcg 1860
atctggagat tctgtagtac cgacaatggg ctgaacccgg atgtcgtgct ggttggcatt 1920
ggagtagagg tgatgttcga ggtcatctac gcggcggcca tcctccgcaa gcgttgtcca 1980
gaactccggg tgcgtgtggt caatgtgacc gacttgatga ttctggagaa ggaaggtcta 2040
catccacatg cattgacgac cgaagctttc gacagtctgt ttggctcgga ccggccgata 2100
cacttcaact accacggata cccgggcgag ctcaaaggtc tgctctttgg gcggccccgc 2160
ctggaccgag tttcagtaga aggatacatg gaggaaggaa gcacgacgac gccgttcgat 2220
atgatgttgc tgaaccgcgt ctcacgatac cacgtggcgc aggcagccgt gatcggggcg 2280
tccagacgga atgagaaggt tcaagttcgg cagcacgaac tggtcagcga attcggccac 2340
aacatcgtgg agacacgcaa atacattctg gccaaccgca aagacccgga tgatacgtat 2400
gatatgccct cctttgaata a 2421
<210> 25
<211> 822
<212> PRT
<213> Penicillium chrysogenum
<400> 25
Met Pro Ala Glu Val Ile Ser Glu Pro Asn Pro Gln Ala Leu Pro Ser
1 5 10 15
His Leu Pro Asp Tyr Leu Glu Lys Leu Ser Val Ser Leu Glu Arg Glu
20 25 30
Lys Leu Asp Glu Lys Thr Tyr Asp Ala Leu Ile Lys Phe Arg Arg Ala
35 40 45
Ala Cys Tyr Ile Ala Ala Ala Met Ile Phe Leu Gln Glu Asn Thr Leu
50 55 60
Leu Lys Ser Glu Leu Thr Phe Gln His Val Lys Pro Arg Leu Leu Gly
65 70 75 80
His Trp Gly Thr Cys Pro Gly Leu Ile Phe Val Tyr Ser His Leu Asn
85 90 95
Tyr Leu Ile Arg Thr Met Asn Leu Asp Met Leu Tyr Val Val Gly Pro
100 105 110
Gly His Gly Ala Pro Ala Ile Leu Ala Ala Leu Trp Leu Glu Gly Ser
115 120 125
Leu Glu Lys Phe Tyr Pro His Tyr Ser Arg Asp Glu Lys Gly Leu His
130 135 140
Arg Leu Ile Ser Thr Phe Ser Thr Thr Gly Gly Phe Pro Ser His Ile
145 150 155 160
Asn Ser Glu Thr Pro Gly Ala Ile His Glu Gly Gly Glu Leu Gly Tyr
165 170 175
Ala Leu Ala Val Ser Phe Gly Ala Val Met Asp Asn Pro Asp Leu Ile
180 185 190
Val Thr Cys Ile Val Gly Asp Gly Glu Ala Glu Ser Gly Pro Thr Ala
195 200 205
Thr Ser Trp His Ala Ile Lys Tyr Ile Asp Pro Lys Glu Ser Gly Ala
210 215 220
Val Leu Pro Ile Leu His Leu Asn Gly Phe Lys Ile Ser Glu Arg Thr
225 230 235 240
Ile Phe Gly Cys Met Asp His Lys Glu Leu Leu Thr Leu Phe Ser Gly
245 250 255
Tyr Gly Tyr Gln Val Arg Phe Val Glu Asp Asn Asn Asp Ile Asp Ala
260 265 270
Asp Leu His Thr Ser Met Ile Trp Ala Val Asn Glu Ile Gln Lys Ile
275 280 285
Gln Lys Ala Ala Arg Ser Gly Lys Pro Ile Met Lys Pro Arg Trp Pro
290 295 300
Met Leu Ile Leu Arg Thr Ser Lys Gly Trp Thr Gly Pro Lys Gln Leu
305 310 315 320
His Gly Lys Phe Leu Glu Gly Ser Tyr His Ser His Gln Val Pro Leu
325 330 335
Pro Lys Ala Lys Thr Asp Lys Glu Gln Leu Asp Leu Leu Gln Asn Trp
340 345 350
Leu Ser Ser Tyr Lys Pro Glu Glu Leu Phe Thr Ser Asn Gly Asp Val
355 360 365
Ile Asp Glu Ile Lys Ser Val Ile Pro Thr Glu Asp Lys Lys Lys Leu
370 375 380
Gly Gln Arg Ile Glu Val Tyr Asn Ser Tyr Thr Pro Pro Asn Leu Pro
385 390 395 400
Asp Trp Lys Pro Phe Cys Ala Asp Lys Gly Ser Gln Glu Ser Ala Met
405 410 415
Lys Ala Ala Gly Thr Phe Ile Asn Gln Thr Phe Lys Asp Asn Pro Asn
420 425 430
Ser Val Arg Leu Phe Ser Pro Asp Glu Leu Glu Ser Asn Lys Leu Asp
435 440 445
Ala Val Phe Glu Tyr Thr Asn Arg Asn Phe Gln Trp Asp Glu Phe Ala
450 455 460
Asn Ala Arg Gly Gly Arg Val Ile Glu Val Leu Ser Glu His Met Cys
465 470 475 480
Gln Gly Phe Met Gln Gly Tyr Thr Leu Thr Gly Arg Ile Gly Ile Phe
485 490 495
Pro Ser Tyr Glu Ser Phe Leu Gly Ile Ile His Thr Met Met Val Gln
500 505 510
Tyr Ala Lys Phe Ile Lys Met Gly Leu Glu Thr Thr Trp His Ser Gly
515 520 525
Val Ser Ser Val Asn Tyr Ile Glu Ser Ser Thr Trp Ala Arg Gln Glu
530 535 540
His Asn Gly Phe Ser His Gln Asn Pro Ser Phe Ile Gly Ala Val Leu
545 550 555 560
Lys Leu Lys Pro Ser Ala Ala Arg Val Tyr Leu Pro Pro Asp Ala Asn
565 570 575
Thr Phe Leu Thr Thr Ile His His Cys Leu Lys Ser Lys Asn Tyr Ile
580 585 590
Asn Leu Met Val Gly Ser Lys Gln Pro Thr Pro Val Tyr Leu Thr Pro
595 600 605
Lys Glu Ala Glu Ser His Cys Arg Ala Gly Ala Ser Ile Trp Lys Phe
610 615 620
Cys Ser Thr Asp Asp Gly Ile Asn Pro Asp Val Val Leu Val Gly Ile
625 630 635 640
Gly Val Glu Val Met Phe Glu Val Ile Ala Ala Ala Ala Leu Leu Arg
645 650 655
Lys Leu Ile Pro Glu Leu Arg Val Cys Val Ile Asn Val Thr Asp Leu
660 665 670
Met Ile Leu Asp Asn Glu Gly Ala His Pro His Ala Leu Ser Thr Glu
675 680 685
Ala Phe Asp Gly Leu Phe Thr Ser Asp Arg Pro Ile His Phe Asn Tyr
690 695 700
His Gly Tyr Pro Thr Glu Leu Gln Gly Leu Leu Phe Gly Arg Pro Arg
705 710 715 720
Leu Asp Arg Val Ser Val Ala Gly Tyr Ile Glu Glu Gly Ser Thr Thr
725 730 735
Thr Pro Phe Asp Met Met Leu Val Asn Arg Val Ser Arg Phe His Val
740 745 750
Ala Gln His Ala Ile Arg Gly Ala Ala Lys Val Asn Glu Lys Val Arg
755 760 765
Val Tyr Gln Gln Glu Leu Asn Ala Gln Leu Glu Ala Ser Met Val Ser
770 775 780
Thr Arg Lys Tyr Ile Val Glu Asn Arg Asp Asp Pro Asp Gly Ile Tyr
785 790 795 800
Asp Met Pro Gln Phe His Ser Phe His Lys Pro Ala Glu Ser Glu Thr
805 810 815
Phe Trp Asn Ile Ala Gln
820
<210> 26
<211> 2469
<212> DNA
<213> Penicillium chrysogenum
<400> 26
atgcctgcag aagttataag cgaaccaaat ccacaagctc tgccgtcgca tctgccagac 60
tatcttgaga aattgagcgt cagcttggaa cgtgagaaac tagatgaaaa gacctacgat 120
gccttgatca agttccgccg agcggcttgt tacattgcgg cagctatgat atttctgcaa 180
gaaaatactc ttttgaagtc agaactcaca tttcaacatg tcaagcctag actactcggt 240
cactggggaa catgcccggg cctaatcttt gtctactctc atttgaacta cctgatccgg 300
acaatgaatc tggacatgtt gtacgttgtc ggtccaggac atggcgcacc ggcaatacta 360
gccgcgctat ggttggaggg ttcgctggag aaattctatc cccactattc gcgagacgaa 420
aagggcctac acagattgat ctcgaccttt agtaccacag gtggttttcc cagccacatc 480
aattccgaga cccccggtgc aatccatgaa ggtggagaac tgggttacgc tctggcagtg 540
tcctttggcg ctgtcatgga taaccccgat ctgattgtga cttgtattgt gggggacggg 600
gaggctgaaa gcggtcccac tgctacgtcc tggcatgcga tcaagtacat tgatccaaag 660
gagtccggtg cagttttacc aattctgcat ttgaatggat tcaagatcag cgagcgcact 720
atcttcgggt gcatggacca caaagagctt ttgactctct tcagtggata cgggtaccag 780
gttcgctttg tcgaggataa caacgatatt gatgcggatt tacacacctc tatgatctgg 840
gccgtcaatg aaatccagaa gatccagaag gctgcccgtt cagggaagcc gattatgaag 900
ccaaggtggc cgatgctgat tctgcgtact tccaagggct ggactgggcc caagcagctt 960
catggcaagt ttcttgaggg ctcataccat tctcaccagg tgccattgcc caaagcaaag 1020
accgacaagg aacaactaga tctgctgcag aattggctgt ctagttataa gccagaagag 1080
ctgttcactt cgaacggtga tgtaattgat gagatcaagt ctgtgatacc cacagaagac 1140
aaaaagaagc ttggccagcg catcgaagtc tacaatagtt atacaccacc gaatctgccg 1200
gattggaagc ccttctgcgc ggataaaggc tctcaagaaa gcgctatgaa agcggctggc 1260
accttcatta atcagacatt caaggacaac ccgaacagcg tgcgactctt ctcgccggac 1320
gagttggaaa gcaacaaact cgatgcggta tttgaataca caaaccgcaa tttccaatgg 1380
gacgagttcg ccaatgcccg cggcggccgt gtaattgaag tccttagcga acatatgtgt 1440
caaggattca tgcagggcta taccttgacc gggcgcattg gtattttccc gtcctatgag 1500
agcttcctcg gcatcattca tacgatgatg gtgcagtatg ccaagttcat caagatggga 1560
ctggaaacaa cttggcactc cggcgtcagt agtgtgaact acatcgaatc gagcacctgg 1620
gcacgccaag aacacaacgg cttctctcac cagaacccat ccttcatcgg cgctgtgcta 1680
aagctgaagc ctagtgcagc tcgggtatac ttaccaccag atgcgaatac tttcctgaca 1740
accatccacc attgtttaaa atcaaagaat tacatcaacc taatggtagg ctcaaagcag 1800
ccaactccag tgtacctgac tcccaaagag gcggaaagcc actgtcgcgc aggagcatcc 1860
atttggaagt tctgcagcac ggacgatgga attaatccgg atgtcgtgct ggtcggtatt 1920
ggtgttgagg tgatgttcga agtaattgcg gcagcggcgc ttctgcgtaa gctcatccca 1980
gagcttcgtg tttgtgtcat taacgtgacg gacctgatga ttcttgataa cgagggtgcg 2040
catccgcatg ccctgtccac tgaggcattc gatggtctct ttacttccga caggcccatt 2100
cacttcaatt accacggcta tccaactgag ctgcagggtt tgctctttgg acgtccccgc 2160
cttgatcgcg tcagtgtcgc cggctatatc gaagagggta gcaccaccac gcctttcgac 2220
atgatgcttg tcaaccgtgt ctcgcgtttc catgtcgccc agcatgctat tcgtggtgct 2280
gccaaagtaa atgagaaggt cagggtgtat cagcaggagc tgaatgctca gcttgaggcg 2340
agtatggtca gcacgaggaa gtatattgtg gaaaaccggg atgaccccga cggcatttat 2400
gacatgccac aattccacag cttccacaaa ccggctgagt ctgagacatt ctggaatatt 2460
gcccagtag 2469
<210> 27
<211> 808
<212> PRT
<213> Trichoderma reesei
<400> 27
Met Pro Gly Glu Val Ile Asp Gln Pro Asn Pro Pro Pro Leu Thr Ser
1 5 10 15
His Leu Pro Asp Thr Ile Glu Glu Leu Ala Val Lys Pro Ser Lys Ala
20 25 30
Pro Leu Ser Asn Leu Asp Leu Val Ser Leu Arg Glu Phe Gln Arg Ala
35 40 45
Ala Cys Tyr Ile Ala Ser Ala Met Ile Phe Leu Lys Asp Asn Val Leu
50 55 60
Leu Asp Arg Glu Leu Arg Phe Glu Asp Val Lys Pro Arg Leu Leu Gly
65 70 75 80
His Trp Gly Thr Cys Pro Gly Leu Ile Leu Ile Trp Ser His Leu Asn
85 90 95
Leu Leu Ile Arg Asp Ser Ser Gln Asp Met Leu Phe Val Ile Gly Pro
100 105 110
Gly His Gly Ala Pro Ala Ala Leu Ala Cys Leu Trp Leu Glu Gly Ser
115 120 125
Leu Glu Arg Phe Tyr Pro Asp Lys Tyr Arg Thr Asp Lys Glu Gly Leu
130 135 140
His Asn Leu Ile Thr Lys Phe Ser Val Pro Thr Gly Phe Pro Ser His
145 150 155 160
Ile Asn Pro Glu Thr Pro Gly Cys Ile His Glu Gly Gly Glu Leu Gly
165 170 175
Tyr Ala Leu Ala Val Ser Phe Gly Ala Val Met Asp Lys Pro Asp Leu
180 185 190
Ile Val Pro Cys Val Val Gly Asp Gly Glu Ala Glu Thr Gly Pro Thr
195 200 205
Ala Ala Ala Trp His Ser Ile Lys Tyr Leu Asp Pro Ala Glu Ser Gly
210 215 220
Ala Val Ile Pro Ile Leu His Val Asn Gly Phe Lys Ile Ser Glu Arg
225 230 235 240
Thr Ile Phe Gly Cys Met Asp Asn Thr Glu Leu Val Leu Leu Phe Ser
245 250 255
Gly Tyr Gly Tyr Glu Val Cys Ile Val Glu Asn Leu Asp Ala Ile Asp
260 265 270
Thr Glu Leu His Thr Ala Leu Phe Trp Ala Leu Ser Glu Ile Lys Arg
275 280 285
Ile Gln Gly Ala Ala Arg Ser Gly Asn Pro Ile Thr Lys Pro Arg Trp
290 295 300
Pro Met Ile Ile Leu Arg Thr Pro Lys Gly Trp Thr Gly Pro Arg Thr
305 310 315 320
Val Asp Asp Lys Ile Ile Glu Gly Ser Phe His Ala His Gln Val Pro
325 330 335
Val Thr Lys Ala Asn Lys Asp Glu Gly His Leu Arg Ile Leu Gln Asp
340 345 350
Trp Leu Lys Ser Tyr Asp Val Arg Gly Leu Leu Pro Asp Gly Lys Pro
355 360 365
Ser Gly Asp Phe Leu Asp Ile Leu Pro Pro Asp Pro His Lys Arg Leu
370 375 380
Gly Gln Ser Lys Leu Ala Tyr Asp Cys His Gln Pro Leu Asp Leu Pro
385 390 395 400
Asp Trp Arg Pro His Ser Val Asp Lys Phe Glu Glu Ala Ser Ser Met
405 410 415
Gln Gln Ser Gly Lys Phe Leu Asp Val Val Ala Arg Gln Asn Met Lys
420 425 430
Thr Phe Arg Ile Phe Ser Pro Asp Glu Leu Glu Ser Asn Lys Leu Ser
435 440 445
Ala Val Leu Asp His Ser Ser Arg Asn Phe Gln Trp Asp Gln Tyr Ser
450 455 460
Arg Ala Gln Gly Gly Arg Val Ile Glu Ile Leu Ser Glu His Cys Cys
465 470 475 480
Gln Gly Phe Leu Gln Gly Tyr Thr Leu Thr Gly Arg Thr Ala Ile Phe
485 490 495
Pro Ser Tyr Glu Ser Phe Leu Gly Ile Ile His Thr Met Met Ile Gln
500 505 510
Tyr Ser Lys Phe Ser Lys Ile Ser Arg Lys Leu Pro Trp Arg Gly Asp
515 520 525
Leu Ser Ser Ile Asn Tyr Ile Glu Thr Ser Thr Trp Ala Arg Gln Glu
530 535 540
His Asn Gly Phe Ser His Gln Asn Pro Ser Phe Ile Gly Ala Val Leu
545 550 555 560
Asn Leu Lys Ala Glu Ile Ala Arg Val Tyr Leu Pro Pro Asp Ala Asn
565 570 575
Cys Phe Leu Ser Thr Leu His His Cys Leu Gln Ser Lys Asn Tyr Val
580 585 590
Asn Leu Met Ile Gly Ser Lys Gln Pro Thr Pro Val Tyr Leu Ser Ala
595 600 605
Glu Asp Ala Gln Arg His Cys Glu Asp Gly Ala Ser Ile Trp Arg Trp
610 615 620
Ala Ser Thr His Asp Gly Glu His Pro Asp Val Val Leu Val Gly Ile
625 630 635 640
Gly Val Glu Val Thr Phe Glu Val Ile Lys Ala Ala Gln Leu Leu Ser
645 650 655
Arg Leu Ala Pro Asn Leu Arg Val Arg Val Val Asn Val Thr Asp Leu
660 665 670
Leu Val Leu Pro His Glu Ser His His Pro His Ala Leu Asp Ser Lys
675 680 685
Ala Phe Glu Asp Met Phe Thr Leu Asp Lys Pro Val Cys Phe Asn Tyr
690 695 700
His Ser Tyr Ala Thr Glu Leu Gln Gly Leu Leu Phe Gly Arg Pro Ala
705 710 715 720
Leu His Arg Met Ser Val Glu Gly Tyr Lys Glu Glu Gly Ser Thr Thr
725 730 735
Thr Pro Phe Asp Met Met Leu Val Asn Thr Val Ser Arg Phe His Val
740 745 750
Ala Ser Arg Ala Leu Lys Ala Ala Ala Ala Gln Asn Asp Glu Val Lys
755 760 765
Glu Asn Leu Ser Ala Leu Leu Ala Lys Val Asp Asp Glu Met Lys Ser
770 775 780
Val Lys Asp Tyr Ile Glu Gln Trp Gly Lys Asp Pro Asp Asp Ile Tyr
785 790 795 800
Glu Leu Asp Phe Leu Lys Lys Asp
805
<210> 28
<211> 2427
<212> DNA
<213> Trichoderma reesei
<400> 28
atgcctggag aagtcattga tcaaccgaac cccccgccgc tcacgagcca tctcccggat 60
acgatagagg agctggctgt caagccctca aaggcaccgc tgagcaacct cgacttggtc 120
tcgctcagag agttccaacg ggcagcatgt tacattgcga gcgccatgat attcctcaag 180
gacaatgtcc ttttggaccg cgagctacgg tttgaagatg tgaaacccag gcttctaggc 240
cactggggta cctgccctgg tctcatcctc atctggtcac atttgaatct ccttatccgc 300
gacagttccc aggacatgct ctttgtcatt ggacctggtc atggagcccc agctgccttg 360
gcctgcctct ggctcgaagg ttcacttgaa cgattctatc cagacaaata cagaaccgat 420
aaagagggac tgcataacct cattacgaaa ttctctgttc ctacagggtt tccaagccac 480
atcaacccgg agactccagg ttgcatccac gagggcggag agctgggata tgcgttggca 540
gtgtcctttg gagcagtcat ggataagcca gacctcattg ttccatgtgt tgtcggcgat 600
ggcgaggccg agactggccc tacggcagcc gcatggcatt ccatcaagta cttggatccc 660
gcagagtctg gagcagtcat ccccatcctg cacgtcaacg gcttcaagat cagcgagcgg 720
accatcttcg gctgcatgga caacacagag ctcgtcctgc tcttctcggg ctacggctac 780
gaggtgtgca tcgtcgagaa cctggatgcc atagacaccg aactgcatac ggcactattc 840
tgggccctat cagaaatcaa gaggatccaa ggagccgcgc gatcaggaaa ccccatcacc 900
aagccccggt ggcccatgat catcctccga acgccaaagg gatggacagg accccgaacg 960
gtcgacgaca agatcatcga gggctcattc cacgcgcacc aggtgccggt gacaaaggcc 1020
aacaaagacg agggccatct tcgcatcctg caagactggc tcaagagcta cgacgtgcgc 1080
ggactgctcc ccgacggcaa gccttcaggc gacttcctcg acattctgcc gccagaccct 1140
cacaagaggc tgggtcagtc caagcttgcg tacgactgcc atcagcccct cgatctgccg 1200
gactggaggc cgcattcggt ggacaagttt gaagaagcca gcagcatgca gcagtcgggc 1260
aagttcctcg acgtcgtggc gaggcagaac atgaagacgt tccgcatctt ctcccccgac 1320
gagctggaga gcaacaagct gagcgcagtc ctggaccact cgagccgaaa cttccaatgg 1380
gaccagtact cccgcgcgca gggcggccgc gtcattgaga tcctgtccga gcactgctgt 1440
caggggttcc tccagggata caccctgacg gggcgaacgg ccatcttccc cagctacgag 1500
tcgttcctgg gcatcatcca caccatgatg attcagtact ccaaattctc caagatcagc 1560
cgcaagctgc cctggcgagg cgacctgagc tccatcaact acatcgagac gagcacctgg 1620
gcgcgacagg agcacaacgg cttctcgcac cagaaccctt ccttcatcgg cgccgtcctg 1680
aacctcaagg ccgaaatcgc acgggtctac ctgccccctg acgcaaactg cttcctcagc 1740
acccttcacc actgcctcca gtccaagaac tacgtcaacc tgatgattgg ctccaagcag 1800
cccacgcccg tctacctctc tgccgaagac gcccagaggc attgtgaaga cggcgcctcc 1860
atctggagat gggcaagcac ccacgacggc gagcacccag acgtggtgct cgtcggaatc 1920
ggcgtcgagg tgacctttga agtcatcaaa gccgcccaac tcctctccag gctcgcaccg 1980
aatctgcgcg tccgcgtcgt caacgtgacc gatctcctgg tcctccccca cgaatcgcac 2040
caccctcacg ccctcgactc caaggccttt gaagacatgt tcacgctcga caagcccgtg 2100
tgcttcaact accacagcta cgcgacggag ctgcaggggc ttctgtttgg ccgcccggcg 2160
ctgcatcgca tgagcgtgga gggctacaag gaggagggca gcacgacgac gccgtttgac 2220
atgatgcttg tgaataccgt cagccgcttc catgtggcgt cgagggcatt gaaagctgcg 2280
gctgcgcaaa acgacgaggt caaggagaat ctgtctgcct tgttggccaa ggttgacgac 2340
gagatgaaga gcgtaaagga ctatatcgag cagtggggga aagacccaga tgatatttac 2400
gaattggatt tcttgaagaa ggattag 2427
<210> 29
<211> 787
<212> PRT
<213> Lactobacillus curvatus
<400> 29
Met Thr Asp Tyr Ser Ser Lys Glu Tyr Leu Ala Lys Val Asp Ala Phe
1 5 10 15
Trp Arg Ala Ala Asn Tyr Ile Ser Val Gly Gln Leu Tyr Leu Lys Asp
20 25 30
Asn Pro Leu Leu Lys Cys Pro Leu Glu Ala Lys Asp Val Lys Ala Lys
35 40 45
Pro Ile Gly His Trp Gly Thr Ile Ser Gly Gln Asn Phe Leu Tyr Ala
50 55 60
His Leu Asn Arg Ala Ile Asn Lys Tyr Asn Leu Asn Met Phe Tyr Val
65 70 75 80
Glu Gly Pro Gly His Gly Gly Gln Val Met Val Ser Asn Ser Tyr Leu
85 90 95
Asp Gly Ser Tyr Ser Glu Ile Tyr Pro Glu Ile Ser Gln Asp Val Glu
100 105 110
Gly Met Lys Lys Leu Phe Lys Gln Phe Ser Phe Pro Gly Gly Val Ala
115 120 125
Ser His Ala Ala Pro Glu Thr Pro Gly Ser Ile His Glu Gly Gly Glu
130 135 140
Leu Gly Tyr Ser Leu Ser His Gly Val Gly Ala Ile Leu Asp Asn Pro
145 150 155 160
Asp Val Ile Ala Ala Val Val Val Gly Asp Gly Glu Ala Glu Thr Gly
165 170 175
Pro Leu Ala Ala Ser Trp Leu Ser Ser Thr Phe Ile Asn Pro Lys Asn
180 185 190
Asp Gly Ala Val Leu Pro Ile Leu Asn Leu Asn Gly Phe Lys Ile Ser
195 200 205
Asn Pro Thr Ile Leu Ser Arg Lys Ser Asp Glu Glu Leu Thr Lys Tyr
210 215 220
Phe Glu Gly Asn Gly Trp Glu Pro Ile Phe Val Glu Gly Asp Asp Pro
225 230 235 240
Glu Lys Met His Pro Ala Thr Ala Ala Ala Met Asp Glu Ala Ile Glu
245 250 255
Lys Ile Gln Ala Ile Gln Lys Asn Ala Arg Glu Asn Gly Asp Ser Ser
260 265 270
Arg Pro Val Trp Pro Met Ile Val Phe Arg Ala Pro Lys Gly Trp Thr
275 280 285
Gly Pro Lys Thr Trp Asp Gly Ala Pro Ile Glu Asn Ser Phe Arg Ala
290 295 300
His Gln Ile Pro Val Pro Ile Asp Ser Ala Asp Met Gln His Val Asp
305 310 315 320
Ala Leu Val Asp Trp Met Lys Ser Tyr Arg Pro Glu Glu Leu Phe Thr
325 330 335
Glu Glu Gly Gln Leu Lys Pro Glu Ile Ala Ala Ile Ala Pro Lys Gly
340 345 350
Asp Gln Arg Met Ala Ala Asn Pro Ile Thr Asn Gly Gly Ile Asp Pro
355 360 365
Lys Pro Leu Arg Leu Pro Asp Tyr Arg Asp Tyr Ala Val Asp Asn Ser
370 375 380
Glu His Gly Lys Val Val Ala Gln Asp Met Ile Val Leu Gly Glu Tyr
385 390 395 400
Val Arg Asp Ile Ile Lys Asp Asn Asp Gln Asn Lys Asn Phe Arg Ile
405 410 415
Phe Gly Pro Asp Glu Thr Met Ser Asn Arg Leu Asn His Ile Phe Glu
420 425 430
Ala Thr Asn Arg Gln Trp Met Glu Pro Ile Lys Glu Pro Asn Asp Gln
435 440 445
Tyr Met Ala Thr Glu Gly Arg Val Leu Asp Ser Gln Leu Ser Glu His
450 455 460
Gln Ala Glu Gly Trp Leu Glu Gly Tyr Val Leu Thr Gly Arg His Gly
465 470 475 480
Phe Phe Ala Ser Tyr Glu Ser Phe Leu Arg Val Val Asp Ser Met Leu
485 490 495
Thr Gln His Phe Lys Trp Leu Arg Lys Ala Asp Glu Gln Ala Trp Arg
500 505 510
Asn Lys Tyr Pro Ser Leu Asn Val Ile Ala Thr Ser Thr Val Phe Gln
515 520 525
Gln Asp His Asn Gly Tyr Thr His Gln Asp Pro Gly Ile Leu Thr His
530 535 540
Leu Ala Glu Lys Lys Pro Glu Phe Ile Arg Glu Tyr Leu Pro Ala Asp
545 550 555 560
Ala Asn Thr Leu Leu Ala Thr Met Asp Thr Val Phe Lys Ser Gln Glu
565 570 575
Lys Ile Asn Leu Val Val Ala Ser Lys His Pro Arg Gln Gln Trp Phe
580 585 590
Ser Ile Asp Glu Ala Thr Val Leu Val Lys Asn Gly Leu Lys Ile Ile
595 600 605
Asp Trp Ala Ser Thr Asp Gln Asp Ala Glu Pro Asp Val Val Ile Ala
610 615 620
Ala Ala Gly Thr Glu Pro Thr Leu Glu Ser Leu Ala Ala Ile Ser Ile
625 630 635 640
Leu His Lys Gln Tyr Pro Asp Met Lys Ile Arg Phe Ile Asn Val Val
645 650 655
Asp Leu Leu Lys Leu Arg Ser Pro Lys Val Asp Pro Arg Gly Leu Thr
660 665 670
Asp Glu Glu Phe Asp Met Tyr Phe Thr Lys Asp Lys Pro Val Val Phe
675 680 685
Ala Phe His Gly Phe Glu Gly Leu Val Arg Asp Ile Phe Phe Asp Arg
690 695 700
His Asn His Asn Leu His Val His Gly Tyr Arg Glu Asn Gly Asp Ile
705 710 715 720
Thr Thr Pro Phe Asp Met Arg Val Leu Asn Gln Met Asp Arg Phe Ser
725 730 735
Leu Ser Lys Glu Val Ala Val Asp Val Leu Gly Asp Gln Ala Gly Gln
740 745 750
Phe Ala Gln Ser Met Asp Asp Met Val Ala Lys His Asn Gln Tyr Ile
755 760 765
Arg Asp Glu Gly Thr Asp Leu Pro Glu Val Glu Glu Trp Gln Trp Glu
770 775 780
Pro Leu Arg
785
<210> 30
<211> 2364
<212> DNA
<213> Lactobacillus curvatus
<400> 30
atgacagatt attcaagcaa agaatacctt gctaaagttg acgcattttg gcgggctgca 60
aattatatct cagttggtca attatactta aaagataacc cactattgaa atgtcctttg 120
gaagcaaaag atgttaaggc aaaaccaatt ggtcactggg ggacaatctc aggccaaaac 180
ttcctatatg ctcatttaaa ccgtgcaatt aataaatata acttaaacat gttctacgtt 240
gaaggcccag gtcacggtgg tcaagtgatg gtctcaaact catatttaga tggtagctat 300
tcagaaatct atccagaaat ttctcaagat gttgaaggga tgaagaaatt attcaaacaa 360
ttctcattcc caggcggcgt tgcttctcat gcggctcctg aaacacctgg ttcaatccat 420
gaaggtggcg aacttggtta ttcattatca cacggtgttg gtgcgatctt agataaccca 480
gacgtgattg ctgctgttgt tgtgggtgat ggggaagctg aaactggccc attagctgca 540
tcatggttat caagcacatt catcaatcct aagaatgatg gtgctgtctt accaatcttg 600
aacttaaacg gtttcaagat ttctaaccca acaattcttt cacgcaagag tgatgaagaa 660
ttaacgaaat acttcgaagg caatggttgg gaaccaatct ttgttgaagg cgacgaccct 720
gaaaagatgc acccagcaac tgctgctgca atggatgaag ccatcgaaaa aatccaagca 780
attcaaaaga atgcgcgtga aaatggcgat tcatctcgtc cagtatggcc aatgatcgtc 840
ttccgcgcac ctaagggttg gactggtcct aagacatggg atggcgcacc aatcgaaaat 900
tcattccgcg cgcaccaaat tccagtgcca atcgacagtg ctgacatgca acacgttgat 960
gcattagtag actggatgaa gtcataccgt ccagaagaat tgtttacaga agaaggccaa 1020
ttaaaacctg aaatcgcagc aattgcacct aaaggcgatc aacggatggc tgctaaccca 1080
attaccaatg gtgggattga tcctaaacca ttacgcttac cagattaccg tgattatgct 1140
gtggataatt cagaacacgg taaagtcgta gcgcaagaca tgattgtttt aggcgaatat 1200
gttcgtgata tcatcaaaga caacgatcaa aataaaaact tcagaatctt cggccctgac 1260
gaaacaatgt caaaccgttt gaaccatatt tttgaagcaa caaaccgtca atggatggaa 1320
ccaatcaaag aaccaaacga tcaatacatg gcaacagaag gccgtgttct tgattcacaa 1380
ttatcagaac atcaagctga aggttggtta gaaggttatg tcctaactgg tcgtcatggt 1440
ttctttgcaa gttacgaatc attcttgcgc gttgtggatt caatgttaac acaacacttc 1500
aagtggttac gtaaggctga tgaacaagca tggcgtaaca agtatccttc attgaacgtg 1560
attgccacat caactgtttt ccaacaagat cacaatggtt acacacatca agatccaggt 1620
atcttaacac acttagctga aaagaaacct gaatttattc gtgaatactt acctgctgat 1680
gctaacacgt tgttagcaac aatggatact gtcttcaaga gtcaagaaaa gatcaattta 1740
gtggttgctt caaaacaccc acgtcaacaa tggttctcaa ttgacgaagc aacagtctta 1800
gttaaaaatg gtttgaagat tattgactgg gcaagtacag accaagatgc tgaaccagat 1860
gttgtgattg cagctgctgg gacagaacca acacttgaaa gtttggctgc aatctcaatc 1920
ttgcacaaac aatatcctga catgaagatt cgtttcatta acgttgttga tttattgaaa 1980
cttcgttcac ctaaagtgga ccctcgtggt ttaacagatg aagaattcga catgtacttt 2040
acaaaagaca aaccagtggt tttcgcattc cacggtttcg aaggcttagt acgtgatatc 2100
ttctttgacc gtcacaacca taaccttcat gttcatggct accgtgaaaa tggtgacatt 2160
acaacaccat ttgacatgcg tgtcttgaat caaatggatc gtttcagtct atctaaagaa 2220
gttgcagttg acgtacttgg cgatcaagct ggtcaattcg cacaatcaat ggatgacatg 2280
gttgctaaac acaatcaata cattcgcgat gaaggtactg acttaccaga agttgaagaa 2340
tggcaatggg aaccactccg ttaa 2364
<210> 31
<211> 825
<212> PRT
<213> Bifidobacterium adolescentis
<400> 31
Met Thr Ser Pro Val Ile Gly Thr Pro Trp Lys Lys Leu Asn Ala Pro
1 5 10 15
Val Ser Glu Glu Ala Ile Glu Gly Val Asp Lys Tyr Trp Arg Ala Ala
20 25 30
Asn Tyr Leu Ser Ile Gly Gln Ile Tyr Leu Arg Ser Asn Pro Leu Met
35 40 45
Lys Glu Pro Phe Thr Arg Glu Asp Val Lys His Arg Leu Val Gly His
50 55 60
Trp Gly Thr Thr Pro Gly Leu Asn Phe Leu Ile Gly His Ile Asn Arg
65 70 75 80
Leu Ile Ala Asp His Gln Gln Asn Thr Val Ile Ile Met Gly Pro Gly
85 90 95
His Gly Gly Pro Ala Gly Thr Ala Gln Ser Tyr Leu Asp Gly Thr Tyr
100 105 110
Thr Glu Tyr Phe Pro Asn Ile Thr Lys Asp Glu Ala Gly Leu Gln Lys
115 120 125
Phe Phe Arg Gln Phe Ser Tyr Pro Gly Gly Ile Pro Ser His Tyr Ala
130 135 140
Pro Glu Thr Pro Gly Ser Ile His Glu Gly Gly Glu Leu Gly Tyr Ala
145 150 155 160
Leu Ser His Ala Tyr Gly Ala Val Met Asn Asn Pro Ser Leu Phe Val
165 170 175
Pro Ala Ile Val Gly Asp Gly Glu Ala Glu Thr Gly Pro Leu Ala Thr
180 185 190
Gly Trp Gln Ser Asn Lys Leu Ile Asn Pro Arg Thr Asp Gly Ile Val
195 200 205
Leu Pro Ile Leu His Leu Asn Gly Tyr Lys Ile Ala Asn Pro Thr Ile
210 215 220
Leu Ser Arg Ile Ser Asp Glu Glu Leu His Glu Phe Phe His Gly Met
225 230 235 240
Gly Tyr Glu Pro Tyr Glu Phe Val Ala Gly Phe Asp Asn Glu Asp His
245 250 255
Leu Ser Ile His Arg Arg Phe Ala Glu Leu Phe Glu Thr Val Phe Asp
260 265 270
Glu Ile Cys Asp Ile Lys Ala Ala Ala Gln Thr Asp Asp Met Thr Arg
275 280 285
Pro Phe Tyr Pro Met Ile Ile Phe Arg Thr Pro Lys Gly Trp Thr Cys
290 295 300
Pro Lys Phe Ile Asp Gly Lys Lys Thr Glu Gly Ser Trp Arg Ser His
305 310 315 320
Gln Val Pro Leu Ala Ser Ala Arg Asp Thr Glu Ala His Phe Glu Val
325 330 335
Leu Lys Asn Trp Leu Glu Ser Tyr Lys Pro Glu Glu Leu Phe Asp Glu
340 345 350
Asn Gly Ala Val Lys Pro Glu Val Thr Ala Phe Met Pro Thr Gly Glu
355 360 365
Leu Arg Ile Gly Glu Asn Pro Asn Ala Asn Gly Gly Arg Ile Arg Glu
370 375 380
Glu Leu Lys Leu Pro Lys Leu Glu Asp Tyr Glu Val Lys Glu Val Ala
385 390 395 400
Glu Tyr Gly His Gly Trp Gly Gln Leu Glu Ala Thr Arg Arg Leu Gly
405 410 415
Val Tyr Thr Arg Asp Ile Ile Lys Asn Asn Pro Asp Ser Phe Arg Ile
420 425 430
Phe Gly Pro Asp Glu Thr Ala Ser Asn Arg Leu Gln Ala Ala Tyr Asp
435 440 445
Val Thr Asn Lys Gln Trp Asp Ala Gly Tyr Leu Ser Ala Gln Val Asp
450 455 460
Glu His Met Ala Val Thr Gly Gln Val Thr Glu Gln Leu Ser Glu His
465 470 475 480
Gln Met Glu Gly Phe Leu Glu Gly Tyr Leu Leu Thr Gly Arg His Gly
485 490 495
Ile Trp Ser Ser Tyr Glu Ser Phe Val His Val Ile Asp Ser Met Leu
500 505 510
Asn Gln His Ala Lys Trp Leu Glu Ala Thr Val Arg Glu Ile Pro Trp
515 520 525
Arg Lys Pro Ile Ser Ser Met Asn Leu Leu Val Ser Ser His Val Trp
530 535 540
Arg Gln Asp His Asn Gly Phe Ser His Gln Asp Pro Gly Val Thr Ser
545 550 555 560
Val Leu Leu Asn Lys Cys Phe Asn Asn Asp His Val Ile Gly Ile Tyr
565 570 575
Phe Pro Val Asp Ser Asn Met Leu Leu Ala Val Ala Glu Lys Cys Tyr
580 585 590
Lys Ser Thr Asn Lys Ile Asn Ala Ile Ile Ala Gly Lys Gln Pro Ala
595 600 605
Ala Thr Trp Leu Thr Leu Asp Glu Ala Arg Ala Glu Leu Glu Lys Gly
610 615 620
Ala Ala Glu Trp Lys Trp Ala Ser Asn Val Lys Ser Asn Asp Glu Ala
625 630 635 640
Gln Ile Val Leu Ala Ala Thr Gly Asp Val Pro Thr Gln Glu Ile Met
645 650 655
Ala Ala Ala Asp Lys Leu Gly Ala Met Gly Ile Lys Phe Lys Val Val
660 665 670
Asn Val Val Asp Leu Val Lys Leu Gln Ser Ala Lys Glu Asn Asn Glu
675 680 685
Ala Leu Ser Asp Glu Glu Phe Ala Glu Leu Phe Thr Glu Asp Lys Pro
690 695 700
Val Leu Phe Ala Tyr His Ser Tyr Ala Arg Asp Val Arg Gly Leu Ile
705 710 715 720
Tyr Asp Arg Pro Asn His Asp Asn Phe Asn Val His Gly Tyr Glu Glu
725 730 735
Gln Gly Ser Thr Thr Thr Pro Tyr Asp Met Val Arg Val Asn Asn Ile
740 745 750
Asp Arg Tyr Glu Leu Gln Ala Glu Ala Leu Arg Met Ile Asp Ala Asp
755 760 765
Lys Tyr Ala Asp Lys Ile Asn Glu Leu Glu Ala Phe Arg Gln Glu Ala
770 775 780
Phe Gln Phe Ala Val Asp Asn Gly Tyr Asp His Pro Asp Tyr Thr Asp
785 790 795 800
Trp Val Tyr Ser Gly Val Asn Thr Asn Lys Gln Gly Ala Ile Ser Ala
805 810 815
Thr Ala Ala Thr Ala Gly Asp Asn Glu
820 825
<210> 32
<211> 2478
<212> DNA
<213> Bifidobacterium adolescentis
<400> 32
atgacgagtc ctgttattgg caccccttgg aagaagctga acgctccggt ttccgaggaa 60
gctatcgaag gcgtggataa gtactggcgc gcagccaact acctctccat cggccagatc 120
tatctgcgta gcaacccgct gatgaaggag cctttcaccc gcgaagacgt caagcaccgt 180
ctggtcggtc actggggcac caccccgggc ctgaacttcc tcatcggcca catcaaccgt 240
ctcattgctg atcaccagca gaacactgtg atcatcatgg gcccgggcca cggcggcccg 300
gctggtaccg ctcagtccta cctggacggc acctacaccg agtacttccc gaacatcacc 360
aaggatgagg ctggcctgca gaagttcttc cgccagttct cctacccggg tggcatcccg 420
tcccactacg ctccggagac cccgggctcc atccacgaag gcggcgagct gggttacgcc 480
ctgtcccacg cctacggcgc tgtgatgaac aacccgagcc tgttcgtccc ggccatcgtc 540
ggcgacggtg aagctgagac cggcccgctg gccaccggct ggcagtccaa caagctcatc 600
aacccgcgca ccgacggtat cgtgctgccg atcctgcacc tcaacggcta caagatcgcc 660
aacccgacca tcctgtcccg catctccgac gaagagctcc acgagttctt ccacggcatg 720
ggctatgagc cgtacgagtt cgtcgctggc ttcgacaacg aggatcacct gtcgatccac 780
cgtcgtttcg ccgagctgtt cgagaccgtc ttcgacgaga tctgcgacat caaggccgcc 840
gctcagaccg acgacatgac tcgtccgttc tacccgatga tcatcttccg taccccgaag 900
ggctggacct gcccgaagtt catcgacggc aagaagaccg agggctcctg gcgttcccac 960
caggtgccgc tggcttccgc ccgcgatacc gaggcccact tcgaggtcct caagaactgg 1020
ctcgagtcct acaagccgga agagctgttc gacgagaacg gcgccgtgaa gccggaagtc 1080
accgccttca tgccgaccgg cgaactgcgc atcggtgaga acccgaacgc caacggtggc 1140
cgcatccgcg aagagctgaa gctgccgaag ctggaagact acgaggtcaa ggaagtcgcc 1200
gagtacggcc acggctgggg ccagctcgag gccacccgtc gtctgggcgt ctacacccgc 1260
gacatcatca agaacaaccc ggactccttc cgtatcttcg gaccggatga gaccgcttcc 1320
aaccgtctgc aggccgctta cgacgtcacc aacaagcagt gggacgccgg ctacctgtcc 1380
gctcaggtcg acgagcacat ggctgtcacc ggccaggtca ccgagcagct ttccgagcac 1440
cagatggaag gcttcctcga gggctacctg ctgaccggcc gtcacggcat ctggagctcc 1500
tatgagtcct tcgtgcacgt gatcgactcc atgctgaacc agcacgccaa gtggctcgag 1560
gctaccgtcc gcgagattcc gtggcgcaag ccgatctcct ccatgaacct gctcgtctcc 1620
tcccacgtgt ggcgtcagga tcacaacggc ttctcccacc aggatccggg tgtcacctcc 1680
gtcctgctga acaagtgctt caacaacgat cacgtgatcg gcatctactt cccggtggat 1740
tccaacatgc tgctcgctgt ggctgagaag tgctacaagt ccaccaacaa gatcaacgcc 1800
atcatcgccg gcaagcagcc ggccgccacc tggctgaccc tggacgaagc tcgcgccgag 1860
ctcgagaagg gtgctgccga gtggaagtgg gcttccaacg tgaagtccaa cgatgaggct 1920
cagatcgtgc tcgccgccac cggtgatgtt ccgactcagg aaatcatggc cgctgccgac 1980
aagctgggcg ccatgggcat caagttcaag gtcgtcaacg tggttgacct ggtcaagctg 2040
cagtccgcca aggagaacaa cgaggccctc tccgatgagg agttcgctga gctgttcacc 2100
gaggacaagc cggtcctgtt cgcttaccac tcctatgccc gcgatgtgcg tggtctgatc 2160
tacgatcgcc cgaaccacga caacttcaac gttcacggct acgaggagca gggctccacc 2220
accaccccgt acgacatggt tcgcgtgaac aacatcgatc gctacgagct ccaggctgaa 2280
gctctgcgca tgattgacgc tgacaagtac gccgacaaga tcaacgagct cgaggccttc 2340
cgtcaggaag ccttccagtt cgctgtcgac aacggctacg atcacccgga ttacaccgac 2400
tgggtctact ccggtgtcaa caccaacaag cagggtgcta tctccgctac cgccgcaacc 2460
gctggcgaca acgagtga 2478
<210> 33
<211> 733
<212> PRT
<213> Aphanomyces astaci
<400> 33
Met Ser His Gln Phe Thr Thr Lys Ser Val Ala Ser Gln Ser Thr Met
1 5 10 15
Leu Arg Val Arg Pro Phe Leu Ser Ser Arg Lys Ala Ala Ile Thr Leu
20 25 30
Leu Pro Arg Ala Thr Thr Ser Arg Phe Phe Thr Asp Asp Ala Thr Lys
35 40 45
Lys Asn Asp Arg Leu Leu Val Met Thr Asn Gly Gly Val Ala Lys His
50 55 60
Ser His Leu Leu Leu Gly Leu Met Asn Lys Leu Ser Tyr Thr Phe Pro
65 70 75 80
Ser Val Gly Tyr Phe Arg Pro Val Ala Pro Asn Phe His Ser Thr His
85 90 95
Gly Asp His His Val Asp Leu Ile Arg Ser Glu Phe Lys Ile Lys Asp
100 105 110
Glu Pro Tyr Gln Leu Val Gly Met Thr Gln Ala Asp Ile Thr His Ala
115 120 125
His Leu Glu Gly Asp Thr Asp Ser Val Ile Asp Thr Met Leu Ser Lys
130 135 140
Phe Glu Tyr Leu Arg Glu Lys His Asp Phe Val Val Met Glu Gly Ala
145 150 155 160
Val Leu Asp Thr Ser Pro Glu Leu Ser Trp Glu Leu Asn Val Asp Ile
165 170 175
Ala Lys Ser Leu Asn Ala Pro Val Leu Leu Thr Val Asp Ala Asp Asp
180 185 190
Leu Thr Val Asp Pro Ala Leu His Trp Thr Ala Ala Glu Ser Val Ala
195 200 205
Trp Leu Ala Asp Gln Ile Thr Thr Arg Val Leu Leu Ala Lys Asp Met
210 215 220
Ala His Ala Glu Gly Leu Thr His Val Gly Thr Ile Val Asn Arg Val
225 230 235 240
Lys Thr Asp Asp Ala Leu Glu Leu Arg Asp Leu Val His Ala Gln Ile
245 250 255
Lys Ala Arg Gly Phe Asp Pro Thr Lys Leu Leu Gly Ile Leu Pro Leu
260 265 270
Asp Pro Val Leu Asn Ser Lys Arg Leu Asn Glu Val Val Ala Gln Leu
275 280 285
His Ala Lys Gln Leu Tyr Gly Asn Pro Met Ser Asn Ser Val Val Val
290 295 300
Thr Asp Gly Leu Met Ala Thr Thr Glu Leu Lys Asp Leu Phe Lys His
305 310 315 320
Ile Asn Lys His Asp Asp Gly Leu Leu Val Ile Val Ser Ser Glu Arg
325 330 335
Thr Asp Val Ile Leu Gly Leu Leu Ala Ser Arg Leu Ser Gly Ala Leu
340 345 350
Pro Gln Ile Ser Gly Ile Ile Leu Thr Asn Gly Gly Ile Pro Gln Asn
355 360 365
Glu Cys Gln Asp Ile Leu Ile Gly Leu Ala Gln Ile Asp Lys Ala Ser
370 375 380
Val Pro Ile Tyr Ser Val Glu Leu Asp Ser Tyr Arg Thr Ala Ile Ala
385 390 395 400
Leu Ser Lys Val Thr Cys Asp Ile Leu Pro Thr Ser Gln Asn Lys Ile
405 410 415
Gln Gln Ala Tyr Ile Leu Phe Asp Thr Asn Val Glu Ser Asp Glu Leu
420 425 430
Leu Ser His Leu Ile Glu Arg Thr Gly Gly His Gly Arg Thr Pro Lys
435 440 445
Gln Phe Lys His Phe Leu Phe Glu Ala Ser Arg Lys Ala Asp Gln His
450 455 460
Ile Val Leu Thr Glu Gly Glu Asp Asp Arg Ile Leu Gln Ala Ala Asp
465 470 475 480
Glu Val Leu Arg Arg Gly Ile Ala Arg Leu Thr Ile Leu Gly Asp Val
485 490 495
Glu Ser Ile Asn Ala Arg Ala Lys Thr Leu Arg Leu Asp Leu Ser Gln
500 505 510
Ala Thr Leu Leu Asp Pro Ser Lys Ala Asp Lys Leu Ala Thr Tyr Ala
515 520 525
Asp His Tyr Phe Glu Lys Arg Lys Ser Lys Gly Ile Thr Pro Glu Leu
530 535 540
Ala Lys Glu Thr Val Gly Glu Ala Thr Tyr Phe Gly Thr Val Met Val
545 550 555 560
Asp Leu Asp Asp Ala Asp Gly Met Val Ser Gly Val Cys His Thr Thr
565 570 575
Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile Lys Thr Arg Pro Asp
580 585 590
Ile Pro Leu Val Ser Ser Val Phe Phe Met Cys Leu Glu His Asp Val
595 600 605
Val Leu Tyr Gly Asp Cys Ala Val Asn Thr Asp Pro Thr Ala Gln Gln
610 615 620
Leu Ala Gln Ile Ala Val Gln Ser Ala Glu Ser Ala Val Ala Phe Gly
625 630 635 640
Ile Glu Pro Arg Val Ala Leu Leu Ser Tyr Ala Thr Gly Asp Ser Asn
645 650 655
Lys Gly Pro Ile Ile Asp Lys Val Arg Glu Ala Thr Lys Leu Ala Gln
660 665 670
Ser Met Ala Pro Gly Val Ser Ile Tyr Gly Pro Ile Gln Tyr Asp Ala
675 680 685
Ala Thr Asn Pro Ser Ile Ala Lys Gln Lys Val Lys Gly Leu Lys Gln
690 695 700
Ser Glu Met Glu Val Ala Gly His Ala Asn Val Leu Val Phe Pro Asp
705 710 715 720
Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Arg Val
725 730
<210> 34
<211> 2340
<212> DNA
<213> Aphanomyces astaci
<400> 34
atgtcccatc aattcactac caagtctgtt gcttctcaat ctaccatgtt gagagtcaga 60
ccattcttgt catctagaaa ggctgctatt actttgttgc caagagctac tacttctaga 120
ttcttcactg atgatgctac caagaagaac gacagattat tggttatgac taacggtggt 180
gttgctaagc actctcattt gttgttgggt ttgatgaaca agttgtctta cacctttcca 240
tccgttggtt actttagacc agttgctcca aatttccatt ctacccatgg tgatcatcac 300
gttgatttga tcagatccga attcaagatc aaggacgaac catatcaatt ggttggtatg 360
acccaagctg atattactca tgctcatttg gaaggtgata ccgattctgt tattgacacc 420
atgttgtcca agttcgaata cttgagagaa aagcacgatt tcgttgttat ggaaggtgct 480
gttttggata cttctccaga attgtcttgg gaattgaacg ttgatattgc caagtctttg 540
aacgctccag ttttgttgac tgttgatgct gatgatttga cagttgatcc agctttacat 600
tggactgctg ctgaatcagt tgcttggttg gcagatcaaa ttactactag agtcttgttg 660
gctaaggata tggctcatgc tgaaggtttg actcatgttg gtactatcgt taacagagtt 720
aagaccgatg atgccttgga attgagagat ttggttcatg ctcaaatcaa ggctagaggt 780
tttgatccaa ctaagttgtt aggtatcttg ccattggatc cagtcttgaa ctctaagaga 840
ttgaacgaag ttgttgctca attgcacgcc aaacaattat acggtaatcc aatgtccaac 900
tccgttgttg ttactgatgg tttgatggct actaccgaat tgaaggattt gttcaagcac 960
atcaacaagc acgatgacgg tttgttggtt atcgtttctt ctgaaagaac cgatgtcatc 1020
ttgggtttgt tagcttcaag attgtctggt gctttgccac aaatttccgg tattatctta 1080
acaaacggtg gtatcccaca aaacgaatgc caagatattt tgattggttt ggcccaaatt 1140
gataaggcct ctgttccaat ctattccgtt gaattggatt cttacagaac cgctattgcc 1200
ttgtctaagg ttacctgtga tattttgcca acctcccaaa acaaaatcca acaagcctac 1260
attttgttcg acaccaacgt tgaatccgat gaattattgt cccacttgat cgaaagaact 1320
ggtggtcatg gtagaactcc aaagcaattc aaacacttct tgttcgaagc ttccagaaaa 1380
gccgatcaac atatcgtttt gactgaaggt gaagatgaca gaatattgca agctgctgat 1440
gaagttttga gaagaggtat tgctagattg accattttgg gtgatgtcga atctattaac 1500
gctagagcta agaccttgag attggatttg tcacaagcta ccttgttgga cccatctaaa 1560
gctgataagt tggctactta tgccgatcac tacttcgaaa agagaaagtc taaaggtatc 1620
accccagaat tggctaaaga aactgttggt gaagctactt acttcggtac tgttatggtt 1680
gatttggatg atgcagatgg tatggtttct ggtgtttgtc atacaactgc taacactatt 1740
agaccagcct tgcaattgat taagaccaga ccagatattc cattggtttc ttccgttttc 1800
ttcatgtgtt tggaacacga tgttgtcttg tatggtgatt gtgctgttaa tactgatcca 1860
accgctcaac aattggctca aattgctgtt caatcagctg aatctgctgt tgcttttggt 1920
attgaaccta gagttgcttt gttgtcttat gctactggtg attctaacaa gggtccaatc 1980
attgacaaag ttagagaagc tacaaagttg gctcaatcta tggctccagg tgtttctatc 2040
tatggtccta ttcaatatga tgctgccacc aatccatcca ttgctaaaca aaaagttaag 2100
ggtttgaagc aatccgaaat ggaagttgct ggtcatgcta atgttttggt tttcccagat 2160
ttgaacactg gtaacaacac ttacaaggcc gttcaacaat ctactgattg cttggctatt 2220
ggtccaatgt tgcaaggttt gaacaagcca gttaacgatt tgtctagagg tgctacagtt 2280
ggtgatatcg ttactacagt tgcattgact gctatccaag ctaagcaatc taagaactaa 2340
<210> 35
<211> 764
<212> PRT
<213> Aphanomyces invadans
<400> 35
Met Leu Arg Cys Arg Pro Phe Leu Ser Gly Arg Lys Ala Ala Ala Thr
1 5 10 15
Leu Leu Pro Arg Ala Thr Ala Ser Arg Phe Leu Ser Asp Glu Ala Thr
20 25 30
Lys Lys Asn Asp Arg Leu Leu Val Met Thr Asn Gly Ser Val Ala Lys
35 40 45
His Ser His Val Leu Leu Gly Leu Met Asn Lys Leu Ser Tyr Thr Phe
50 55 60
Pro Ser Val Gly Tyr Phe Arg Pro Val Ala Pro Asn Phe His Ser Ser
65 70 75 80
Tyr Asn Asp His His Val Glu Leu Ile Arg Ser Glu Phe Lys Ile Lys
85 90 95
Asp Glu Pro Tyr Gln Leu Val Gly Met Thr Gln Ser Asp Ile Thr His
100 105 110
Ala His Leu Glu Gly Asp Pro Asp Ser Val Ile Glu Thr Met Leu Ser
115 120 125
Lys Phe Glu Asp Leu Arg Ala Lys His Asp Phe Val Val Met Glu Gly
130 135 140
Ala Lys Leu Asp Ser Ser Pro Glu Leu Ser Trp Glu Leu Asn Val Asp
145 150 155 160
Ile Ala Lys Ser Leu Asn Ala Pro Val Leu Leu Thr Val Asp Ala Asp
165 170 175
Asp Leu Val Val Asp Pro Gly Leu His Trp Thr Asp Ala Glu Ala Leu
180 185 190
Ser Trp Leu Ala Asp Gln Ile Thr Thr Arg Val Leu Leu Ala Lys Asp
195 200 205
Met Ala Gln Ala Glu Gly Leu Thr His Val Gly Thr Leu Val Asn Arg
210 215 220
Val Arg Thr Pro Asp Ala Leu Glu Leu Arg Glu His Val His Ala Gln
225 230 235 240
Ile Lys Ala Arg Gly Phe Asp Pro Ser Lys Leu Leu Gly Ile Leu Pro
245 250 255
Val Asp Pro Val Leu Asp Ser Lys Arg Leu Asn Glu Val Val Ala Gln
260 265 270
Leu His Ala Lys Gln Leu Tyr Gly Asn Pro Met Ser Asn Ser Val Val
275 280 285
Val Thr Asp Gly Leu Met Ala Thr Ala Asp Leu Lys Asp Leu Phe Lys
290 295 300
His Ile Asn Lys His Asp Asp Gly Leu Leu Val Ile Val Ser Ala Glu
305 310 315 320
Arg Thr Asp Val Ile Leu Gly Leu Val Ala Ser Arg Leu Ser Gly Ala
325 330 335
Leu Pro Gln Ile Ser Gly Ile Ile Leu Thr Asn Gly Gly Ile Pro Gln
340 345 350
Asn Glu Cys Gln Glu Ile Leu Lys Gly Leu Ser Lys Ile Gly Lys Ala
355 360 365
Ser Val Pro Ile Tyr Ser Val Glu Thr Asp Ser Tyr His Thr Gly Ile
370 375 380
Ala Leu Ser Lys Val Thr Cys Asp Ile Leu Pro Thr Ser Gln Asn Lys
385 390 395 400
Ile Gln His Ala Tyr Ile Leu Phe Asp Lys Asn Val Glu Ser Lys Glu
405 410 415
Leu Leu Ser His Leu Val Glu Gln Ser Gly Gly Asn Arg Arg Thr Pro
420 425 430
Lys Gln Phe Lys His Phe Leu Phe Glu Ala Ser His Lys Ala Asp Gln
435 440 445
His Ile Val Leu Thr Glu Gly Glu Asp Asp Arg Ile Leu Gln Ala Ala
450 455 460
Asp Glu Val Leu Arg Arg Gly Ile Ala Arg Leu Thr Ile Leu Gly Asp
465 470 475 480
Val Asp Ser Ile Asn Ala Arg Ala Lys Thr Leu Arg Leu Asp Leu Ser
485 490 495
Lys Ala Thr Leu Leu Asn Pro Leu Lys Ala Glu Lys Leu Ala Met Tyr
500 505 510
Ala Asp His Tyr Tyr Glu Lys Arg Lys Ala Lys Gly Ile Thr Pro Glu
515 520 525
Leu Ala Lys Glu Thr Val Gly Glu Ala Thr Tyr Phe Gly Thr Val Met
530 535 540
Val Asp Val Asn Asp Ala Asp Gly Met Val Ser Gly Val Cys His Thr
545 550 555 560
Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile Lys Thr Arg Pro
565 570 575
Asp Ile Pro Leu Val Ser Ser Val Phe Phe Met Cys Leu Glu His Asp
580 585 590
Val Val Leu Tyr Gly Asp Cys Ala Val Asn Thr Asp Pro Thr Ala Glu
595 600 605
Gln Leu Ala Gln Ile Ala Val Gln Ser Ala Glu Ser Ala Lys Ala Phe
610 615 620
Asp Ile Glu Pro Arg Val Ala Leu Leu Ser Tyr Ala Thr Gly Asp Ser
625 630 635 640
Asn Lys Gly Pro Ile Ile Asp Lys Val Arg Glu Ala Thr Lys Leu Ala
645 650 655
Gln Thr Met Ala Pro Glu Val Ser Ile Tyr Gly Pro Ile Gln Tyr Asp
660 665 670
Ala Ala Thr Asn Pro Ser Ile Ala Lys Gln Lys Val Lys Gly Leu Lys
675 680 685
Gln Ser Glu Met Asp Val Ala Gly His Ala Asn Val Leu Val Phe Pro
690 695 700
Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln Gln Ser Thr
705 710 715 720
Asp Cys Leu Ala Ile Gly Pro Met Leu Gln Gly Leu Asn Lys Pro Val
725 730 735
Asn Asp Leu Ser Arg Gly Ala Thr Val Gly Asp Ile Val Thr Thr Val
740 745 750
Ala Leu Thr Ala Ile Gln Ala Arg Gln Ser Lys Lys
755 760
<210> 36
<211> 2295
<212> DNA
<213> Aphanomyces invadans
<400> 36
atgttgagat gcagaccatt cttgtctggt agaaaagctg ctgctacttt gttgccaaga 60
gctactgctt ctagattttt gtctgatgaa gctaccaaga agaacgacag attattggtt 120
atgaccaacg gttccgttgc caaacattct catgttttgt tgggtttgat gaacaagttg 180
tcttacacct ttccatccgt tggttacttt agaccagttg ctccaaattt ccactcctct 240
tacaatgatc atcacgtcga attgattaga tccgaattca agatcaagga cgaaccatat 300
caattggttg gtatgaccca atccgatatt actcatgctc atttggaagg tgatccagat 360
tctgttattg aaaccatgtt gtccaagttc gaagatttga gagctaagca cgatttcgtt 420
gttatggaag gtgctaagtt ggattcttct ccagaattgt cttgggaatt gaacgttgat 480
attgccaagt ctttgaacgc tccagttttg ttgactgttg atgctgatga tttggttgtt 540
gatccaggtt tacattggac tgatgctgaa gctttgtctt ggttggctga tcaaattact 600
actagagtct tgttggctaa ggatatggct caagctgaag gtttgactca tgttggtact 660
ttggttaaca gagttagaac tccagatgcc ttggaattga gagaacacgt tcatgctcaa 720
atcaaggcta gaggttttga tccatctaag ttgttgggta tcttgccagt tgatcctgtt 780
ttggattcta agagattgaa cgaagttgtt gctcaattgc acgccaaaca attatacggt 840
aatccaatgt ccaactccgt tgttgttact gatggtttga tggctactgc tgatttgaag 900
gatttgttca agcacatcaa caagcacgat gacggtttgt tggttatagt ttctgctgaa 960
agaaccgatg tcatcttggg tttggttgct tcaagattgt ctggtgcttt gccacaaatt 1020
tccggtatta tcttgactaa cggtggtatc ccacaaaacg aatgccaaga aattttgaaa 1080
ggtttgtcca agattggtaa ggcctctgtt ccaatctatt ctgttgaaac cgattcttac 1140
catactggta ttgccttgtc taaggttacc tgtgatattt tgccaacctc ccaaaacaaa 1200
atccaacatg cctacatctt gttcgacaag aacgtcgaat ccaaagaatt attgtcccac 1260
ttggttgaac aatccggtgg taatagaaga actccaaagc aattcaagca ctttttgttc 1320
gaagcttccc acaaagctga tcaacatatc gttttgactg aaggtgaaga tgacagaata 1380
ttgcaagctg ctgatgaagt tttgagaaga ggtattgcta gattgaccat tttgggtgat 1440
gttgattcca ttaacgctag agctaagacc ttgagattgg atttgtctaa agctaccttg 1500
ttgaacccat tgaaggctga aaaattggct atgtacgctg atcactacta cgaaaaaaga 1560
aaggctaagg gtattacccc agaattggct aaagaaactg ttggtgaagc tacttacttc 1620
ggtactgtta tggttgatgt taatgatgcc gatggtatgg tttctggtgt ttgtcataca 1680
actgctaaca ctattagacc agccttgcaa ttgattaaga ccagaccaga tattccattg 1740
gtttcttccg ttttcttcat gtgtttggaa cacgatgttg tcttgtatgg tgattgtgct 1800
gttaacactg atccaactgc tgaacaatta gctcaaatcg ctgttcaatc tgctgaatct 1860
gctaaggctt ttgatatcga acctagagtt gctttgttgt cttatgctac tggtgattct 1920
aacaagggtc caatcattga taaggttaga gaagctacta agttggctca aactatggct 1980
cctgaagttt ctatctatgg tccaattcaa tacgatgctg ctaccaatcc atctatcgct 2040
aaacaaaagg ttaagggttt gaagcaatcc gaaatggatg ttgctggtca tgctaatgtt 2100
ttggttttcc cagatttgaa cactggtaac aacacttaca aggccgttca acaatctact 2160
gattgcttgg ctattggtcc aatgttgcaa ggtttgaaca agccagttaa tgacttgtct 2220
agaggtgcaa cagttggtga tatagttact actgttgctt tgaccgctat tcaagctaga 2280
caatctaaga agtaa 2295
<210> 37
<211> 550
<212> PRT
<213> Auxenochlorella protothecoides
<400> 37
Met Asp Leu Asn Arg Asp Asn Pro Gly Thr Ala Ser Glu Ile Ala Ser
1 5 10 15
Arg Ala Leu Arg His Arg Arg Glu Leu Gln Ala Asp Arg Ala Ser Val
20 25 30
Leu Gly Leu Leu Leu Asn Lys Val Pro Leu Ala Ala His Ala Asp Ile
35 40 45
Val Ser Gly Val Ala Ala Glu Leu Ala Asp Ser Gly Leu Thr Phe Ala
50 55 60
Gly Gly Ile Pro Tyr Asp Arg Ile Ile Gly Thr Ala Arg Val Asn Glu
65 70 75 80
Phe Ala Asn Ala Leu Gly Ala Lys Gln Ile Tyr Gly Arg Pro Glu Leu
85 90 95
Ile Asp Ser Gly Val Trp Gly Gly Arg Glu Asp Asn Arg Phe Gly Gly
100 105 110
Arg Ser Val His Gly Phe Leu Asp Lys Leu Glu Ala Ile Arg Ala Gln
115 120 125
Arg Glu Ala Glu Gly Gln Thr Phe Phe Arg Pro Leu Val Leu Thr Thr
130 135 140
Lys Asp Arg Gln Asp Leu Val Leu Gly Leu Ala Ala Ala Ser Leu Ser
145 150 155 160
Gly Ala Cys Pro Pro Leu Gly Gly Leu Val Leu Cys Asp Gly Gly Ala
165 170 175
Cys Ala Ile Thr Pro Pro Val His Ala Ile Met Ala Arg Leu Gly Pro
180 185 190
Asp Thr Leu Pro Val Leu Glu Val Pro His Gly Ala Phe Glu Thr Ala
195 200 205
Arg Arg Met Ala Arg Val Asn Pro Gly Ile Leu Pro Thr Ser Val Arg
210 215 220
Lys Val Arg Glu Ala Arg Ala Leu Phe Gly Arg His Val Asp Val Asp
225 230 235 240
Ala Val Ala Ser Gly Met Ala Val His Arg Glu Pro Arg Leu Thr Pro
245 250 255
Lys Arg Phe Pro Gly Glu Val Ala Ala Ala Ala Ala Arg Phe Asn Val
260 265 270
Asp Val Ser Arg Cys Glu Val Glu Asp His Arg Ser Ser Thr Arg Leu
275 280 285
Asp Ala Tyr Ala Asp Phe Leu Val Glu Ala Arg Lys Lys Lys Ala Leu
290 295 300
Thr Lys Ala Ala Ala Leu Asp Gln Leu Thr Asp Ile Asn Met Phe Gly
305 310 315 320
Thr Ile Met Val Ala Met Gly Asp Ala Asp Gly Met Val Ser Gly Ala
325 330 335
Thr Cys Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Val Leu Lys
340 345 350
Thr Pro Asp Arg Arg Leu Ile Ser Ser Val Phe Phe Met Cys Leu Pro
355 360 365
Asp Arg Val Leu Val Tyr Gly Asp Cys Ala Val Asn Val Glu Pro Thr
370 375 380
Ala Ala Glu Leu Ala Gln Ile Ala Thr Thr Ser Ala Asp Thr Ala Ala
385 390 395 400
Ala Phe Gly Val Glu Pro Arg Val Ala Met Leu Ser Tyr Ser Thr Leu
405 410 415
Gly Ser Gly Ala Gly Pro Gln Val Asp Leu Val Thr Glu Ala Thr Ala
420 425 430
Leu Ala Arg Ala Ala Arg Pro Asp Leu Ala Ile Glu Gly Pro Ile Gln
435 440 445
Tyr Asp Ala Ala Val Asp Pro Gly Val Ala Ala Thr Lys Val Lys Gly
450 455 460
Arg Ser Glu Val Ala Gly Arg Ala Thr Val Cys Val Phe Pro Asp Leu
465 470 475 480
Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln Gln Ser Thr Gly Ala
485 490 495
Ile Ala Ile Gly Pro Leu Met Gln Gly Leu Ala Arg Pro Val Asn Asp
500 505 510
Leu Ser Arg Gly Cys Thr Val Ala Asp Ile Val Asn Thr Val Ala Cys
515 520 525
Thr Ala Val Gln Ala Ala Gly Leu Lys Ala Ala Ala Gln Gln Ala Thr
530 535 540
Ser Ala Ala Ala Ala Ala
545 550
<210> 38
<211> 1653
<212> DNA
<213> Auxenochlorella protothecoides
<400> 38
atggacttga acagagataa tccaggtact gcttctgaaa ttgcttctag agctttgaga 60
cacagaagag aattgcaagc tgatagagct tctgttttgg gtttgttgtt gaacaaagtt 120
ccattggctg ctcatgctga tatagtttct ggtgttgctg ctgaattggc tgattctggt 180
ttgacttttg ctggtggtat tccatacgat agaattattg gtactgccag agttaacgaa 240
tttgctaatg ctttgggtgc caaacaaatc tatggtagac cagaattgat tgactctggt 300
gtttggggtg gtagagaaga taatagattt ggtggtagat ccgttcatgg tttcttggat 360
aagttggaag ctattagagc acaaagagaa gctgaaggtc aaactttttt cagaccattg 420
gttttgacca ccaaggatag acaagatttg gttttaggtt tggctgctgc ttctttgtct 480
ggtgcttgtc caccattggg tggtttggtt ttgtgtgatg gtggtgcatg tgctattact 540
ccaccagttc atgctattat ggctagattg ggtccagata ctttgccagt tttggaagtt 600
ccacatggtg cttttgaaac tgctagaaga atggctagag ttaacccagg tattttgcca 660
acttctgtta gaaaggttag agaagctaga gccttgtttg gtagacatgt tgatgttgat 720
gctgttgctt ctggtatggc tgttcataga gaacctagat tgactccaaa aagattccca 780
ggtgaagttg ctgcagctgc tgctagattc aatgttgacg tttctagatg cgaagtcgaa 840
gatcatagat cctctactag attggatgct tacgctgatt ttttggttga agccagaaaa 900
aagaaggctt tgacaaaagc tgctgccttg gatcaattga ccgatattaa catgttcggt 960
actatcatgg ttgctatggg tgatgctgat ggtatggttt caggtgctac ttgtactact 1020
gctaacacta ttagaccagc cttgcaagtt ttgaaaaccc cagatagaag attgatctcc 1080
tccgtttttt tcatgtgctt gccagataga gttttggttt atggtgattg cgctgttaac 1140
gttgaaccta ctgctgcaga attagctcaa attgctacta cttctgctga tactgctgct 1200
gcatttggtg tcgaacctag agttgctatg ttgtcttatt ctactttggg ttctggtgca 1260
ggtccacaag ttgatttggt tactgaagct actgctttgg ctagagctgc tagacctgat 1320
ttggctattg aaggtccaat tcaatatgat gctgctgttg atccaggtgt tgcagctaca 1380
aaagttaagg gtagatctga agttgcaggt agagctacag tttgtgtttt tccagatttg 1440
aacactggta acaacaccta caaagccgtt caacaatcta ctggtgctat tgctattggt 1500
ccattgatgc aaggtttagc tagaccagtt aatgacttgt ctagaggttg tactgttgcc 1560
gatatcgtta atactgttgc ttgtactgct gttcaagctg ctggtttgaa agctgccgct 1620
caacaagcta catctgctgc cgctgcagct taa 1653
<210> 39
<211> 708
<212> PRT
<213> Beauveria bassiana
<400> 39
Met Leu Ile Pro Thr Gly Thr Ser Val Gly Leu Thr Ser Val Ser Leu
1 5 10 15
Gly Val Ile Arg Ala Met Glu Gln Lys Gly Val Arg Leu Ser Val Phe
20 25 30
Lys Pro Ile Ala Gln Pro Arg Thr Gly Gly Asp Ser Pro Asp Gln Thr
35 40 45
Thr Ser Ile Ile Arg Ala Asn Ser Thr Ile Pro Ala Ala Glu Pro Leu
50 55 60
Asn Met Gly His Val Glu Ser Leu Leu Ser Ser Asn Gln Gln Asp Val
65 70 75 80
Leu Met Glu Glu Ile Ile Ala Asn Tyr His Ala Thr Ser Lys Asp Ala
85 90 95
Glu Val Val Leu Val Glu Gly Leu Val Pro Thr Arg Lys His Gln Phe
100 105 110
Ala Gln Ala Leu Asn Tyr Glu Ile Ala Lys Thr Leu Asn Ala Glu Ile
115 120 125
Val Phe Val Met Ser Leu Gly Asn Asp Ser Pro Glu Gln Leu Lys Glu
130 135 140
Arg Ile Glu Leu Thr Arg Ser Ser Phe Gly Gly Ser Lys Asn Thr Asn
145 150 155 160
Ile Thr Gly Val Ile Ile Asn Lys Leu Asn Ala Pro Val Asp Glu Gln
165 170 175
Gly Arg Thr Arg Pro Asp Leu Ser Glu Ile Phe Asp Asp Ser Ser Lys
180 185 190
Ala Ser Ile Ala Asn Ile Asp Pro Lys Gln Leu Phe Ala Asp Ser Pro
195 200 205
Leu Pro Val Leu Gly Cys Val Pro Trp Ser Phe Glu Leu Ile Ala Thr
210 215 220
Arg Ala Ile Asp Met Ala Arg His Leu Asn Ala Thr Ile Val Asn Glu
225 230 235 240
Gly Asp Ile Asn Thr Arg Arg Val Lys Ser Val Thr Phe Cys Ala Arg
245 250 255
Ser Ile Pro His Met Leu Glu His Phe Arg Pro Gly Ser Leu Leu Val
260 265 270
Thr Ser Ala Asp Arg Pro Asp Val Leu Val Ala Ala Cys Leu Ala Ala
275 280 285
Met Asn Gly Val Glu Ile Gly Ala Ile Leu Leu Thr Gly Gly Tyr Glu
290 295 300
Met Asp Pro Arg Ile Ser Lys Leu Cys Glu Arg Ala Phe Ala Thr Gly
305 310 315 320
Leu Pro Leu Phe Met Val Glu Thr Asn Thr Trp Gln Thr Ser Leu Ser
325 330 335
Leu Gln Ser Phe Asn Leu Glu Val Pro Thr Asp Asp His Gln Arg Ile
340 345 350
Glu Lys Val Gln Glu Tyr Val Ala Ser His Ile Asp Ala Asn Trp Ile
355 360 365
Glu Ser Leu Thr Ala Thr Ser Glu Arg Ser Arg Arg Leu Ser Pro Pro
370 375 380
Ala Phe Arg Tyr Gln Leu Thr Glu Leu Ala Arg Lys Ala Gly Lys Arg
385 390 395 400
Val Val Leu Pro Glu Gly Asp Glu Pro Arg Thr Val Lys Ala Ala Ala
405 410 415
Ile Cys Ala Glu Arg Gly Ile Ala Thr Cys Val Leu Leu Gly Asn Pro
420 425 430
Asp Glu Ile Thr Arg Val Ala Ala Ala Gln Gly Val Glu Leu Gly Ala
435 440 445
Gly Ile Glu Ile Val Asp Pro Glu Val Val Arg Glu Ser Tyr Val Ala
450 455 460
Arg Leu Val Glu Leu Arg Lys Ser Lys Gly Met Thr Glu Ala Val Ala
465 470 475 480
Arg Glu Gln Leu Glu Asp Asn Val Val Leu Gly Thr Leu Met Leu Glu
485 490 495
Gln Asp Glu Val Asp Gly Leu Val Ser Gly Ala Val His Thr Thr Ala
500 505 510
Asn Thr Ile Arg Pro Pro Leu Gln Leu Ile Lys Thr Ala Pro Gly Ser
515 520 525
Ser Leu Val Ser Ser Val Phe Phe Met Leu Leu Pro Glu Gln Val Tyr
530 535 540
Val Tyr Gly Asp Cys Ala Ile Asn Pro Asp Pro Thr Ala Glu Gln Leu
545 550 555 560
Ala Glu Ile Ala Ile Gln Ser Ala Asp Ser Ala Ala Ala Phe Gly Ile
565 570 575
Asp Pro Arg Val Ala Met Leu Ser Tyr Ser Thr Gly Asn Ser Gly Ala
580 585 590
Gly Ser Asp Val Glu Lys Val Arg Glu Ala Thr Arg Ile Ala Gln Glu
595 600 605
Lys Arg Pro Asp Leu Val Ile Asp Gly Pro Leu Gln Tyr Asp Ala Ala
610 615 620
Val Met Ala Asp Val Ala Lys Ser Lys Ala Pro Asn Ser Pro Val Ala
625 630 635 640
Gly Arg Ala Thr Val Phe Ile Phe Pro Asp Leu Asn Thr Gly Asn Thr
645 650 655
Thr Tyr Lys Ala Val Gln Arg Ser Ala Asp Leu Ile Ser Ile Gly Pro
660 665 670
Met Leu Gln Gly Met Arg Lys Pro Val Asn Asp Leu Ser Arg Gly Ala
675 680 685
Leu Val Asp Asp Ile Val Tyr Thr Ile Ala Leu Thr Ala Ile Gln Ser
690 695 700
Ala Gln Gln Ala
705
<210> 40
<211> 2127
<212> DNA
<213> Beauveria bassiana
<400> 40
atgttgattc caaccggtac ttctgttggt ttgacttctg tttctttggg tgttattaga 60
gccatggaac aaaagggtgt tagattgtct gttttcaagc caattgctca acctagaact 120
ggtggtgatt ctccagatca aactacctct attatcagag ccaactctac tattccagct 180
gctgaaccat tgaatatggg tcatgttgaa tccttgttgt cctccaatca acaagatgtc 240
ttgatggaag aaattatcgc taactaccat gccacttcta aggatgctga agttgttttg 300
gttgaaggtt tggttccaac tagaaagcac caatttgctc aagccttgaa ttacgaaatt 360
gccaagactt tgaacgccga aatcgttttc gttatgtctt tgggtaatga ctccccagaa 420
caattgaaag aaagaatcga attgaccaga tcctctttcg gtggttctaa aaacactaac 480
attaccggtg tcatcatcaa caagttgaat gctccagttg atgaacaagg tagaactaga 540
ccagatttgt ccgaaatctt cgatgattct tccaaggctt ccattgctaa cattgaccca 600
aagcaattat ttgccgattc tccattgcca gttttgggtt gtgttccttg gtcttttgaa 660
ttgattgcta ccagagctat tgatatggcc agacatttga atgctaccat tgtcaatgaa 720
ggtgacatca acactagaag agttaagtct gttaccttct gcgctagatc tattcctcat 780
atgttggaac atttcagacc aggttctttg ttggttactt ctgctgatag accagacgtt 840
ttagttgctg cttgtttggc tgctatgaac ggtgttgaaa taggtgctat tttgttgact 900
ggtggttacg aaatggatcc aagaatttct aagttgtgcg aaagagcttt tgctactggt 960
ttgcctttgt tcatggttga aactaatact tggcaaacct ccttgtcctt gcaatctttt 1020
aacttggaag ttccaaccga tgaccaccaa agaattgaaa aggttcaaga atacgttgcc 1080
tcccatattg atgctaactg gattgaatct ttgaccgcta cttctgaaag atccagaaga 1140
ttgtctccac cagcttttag ataccaattg actgaattgg ctagaaaggc tggtaaaaga 1200
gttgttttac ctgaaggtga tgaacctaga acagtaaaag ctgctgctat ttgcgctgaa 1260
agaggtattg caacttgcgt tttgttgggt aatccagacg aaattactag agttgctgca 1320
gctcaaggtg ttgaattggg tgcaggtatt gaaatagttg acccagaagt tgtcagagaa 1380
tcttacgttg ctagattggt cgaattgaga aagtctaagg gtatgactga agctgttgcc 1440
agagaacaat tagaagataa tgttgtcttg ggtactttga tgttagaaca agatgaagtc 1500
gacggtttgg tttctggtgc tgttcataca acagctaaca ctattagacc accattgcaa 1560
ttgattaaga ctgctcctgg ttcttcctta gtctcttctg tttttttcat gttgttgcct 1620
gaacaagtct acgtttatgg tgattgtgct attaacccag atccaacagc tgaacaattg 1680
gccgaaattg ctattcaatc tgctgattct gcagctgctt ttggtattga tccaagagtt 1740
gctatgttga gttactctac tggtaattca ggtgctggtt ctgatgttga aaaagttaga 1800
gaagctacca gaatcgccca agaaaaaaga cctgatttgg ttattgatgg tccattacaa 1860
tacgatgctg ctgttatggc agatgttgct aaatctaaag ctccaaattc tccagttgct 1920
ggtagagcta ctgttttcat ttttccagat ttgaacactg gtaacaccac ctacaaagct 1980
gttcaaagaa gtgccgattt gatttccatt ggtccaatgt tgcaaggtat gagaaagcca 2040
gttaacgatt tgtctagagg tgctttggtt gatgatatcg tttacactat tgccttgacc 2100
gcaattcaat cagctcaaca agcttga 2127
<210> 41
<211> 769
<212> PRT
<213> Chlamydomonas reinhardtii
<400> 41
Met Ala Phe Ala Ser Ser Ser Met Ala Ala Leu Ser Arg Pro Leu Ala
1 5 10 15
Ala Val Ser Ser Gly Leu Gly Ser Ala Leu Ser Arg Ala Ser Gln Leu
20 25 30
Leu Thr Ser Gly Ser Leu Ser Ser Ser Pro Thr Ala Ser His Ser Ser
35 40 45
Thr Arg Arg Phe Ile Ser Asp Gly Thr Val Gly Ser Lys Gly Arg Pro
50 55 60
Asp Ser Leu Phe Leu Ser Asp Ile Ser Gly Arg Pro Asn Ala Thr Ala
65 70 75 80
Asn Asp Tyr Tyr Asn Lys Ala Met Val Lys Arg Gln Val Phe Ala Asp
85 90 95
His Lys Val Asp Val Leu Gly Val Val Ile Asn Gly Leu Pro Arg Glu
100 105 110
His His Ala Ile Leu Ser Ser Gln Leu Arg Asp Lys Leu Glu Arg Ala
115 120 125
Gly Leu Pro Phe Ala Gly Ala Leu Pro Glu Asp Pro Val Leu Ser Ser
130 135 140
Val Arg Pro Pro Pro Pro Ala Leu Asp Lys Pro Ser Pro Gly Leu Thr
145 150 155 160
Cys Val Ser Ser Cys Ala Pro Ser His Arg Tyr Pro Pro Thr Leu Pro
165 170 175
Pro Leu Gly Leu Gly Leu Leu Leu Cys Pro Cys Pro Val Pro Ala Arg
180 185 190
Leu Gln Leu Ile Ala Ser Gly Lys Gln Ser Glu Val Leu Asp Arg Ile
195 200 205
Tyr Ala Ala Tyr Met Ala Tyr Lys Ala Gly Gly Glu Leu Asp Leu Cys
210 215 220
Leu Val Glu Gly Pro Gly Pro Leu Met Gly Gly Thr Glu Leu Asp Ala
225 230 235 240
Gln Val Val Val Gly Ser Gln Arg Leu Glu Glu Leu Leu Glu Thr Leu
245 250 255
Val Glu Arg Pro Met Gly Arg Pro Leu Val Val Thr Ser Ala Asp Arg
260 265 270
Leu Asp Ile Val Leu Gly Leu Leu Ala Ala Gln Leu Ser Val Arg Gly
275 280 285
Pro Ser Val Ala Gly Val Leu Leu Thr Gln Ala Gly Ala Ser Arg Ile
290 295 300
Thr Arg Ser Tyr Ala Lys Ser Ala Val Asp Asn Ile Phe Ala Gly Leu
305 310 315 320
Ser Asn Asn Thr Gly Ala Ser Gly Gly Gly Pro Asp Gly Ala Ala Ala
325 330 335
Ala Asn Gly Ser Ala Gln Gly Ser Leu Tyr Arg Gly Ala Leu Leu Pro
340 345 350
Val Leu Ser Thr Asp Lys His Leu Ala Glu Ala Leu Ala Val Ile Gly
355 360 365
Arg Met Asp Ala Ser Ile Leu Pro Thr Ser Ile Arg Lys Val Thr Gln
370 375 380
Cys Lys Val Ala Gly Ala Ala Val Met Leu Phe Asp Lys Tyr Ile Asp
385 390 395 400
Ala Asn Ala Val Val Thr Gly Leu Gln Lys Ser Arg Pro Thr Arg Val
405 410 415
Thr Pro Lys Met Phe Gln His Thr Met Lys Ala Met Cys Arg Ala Ser
420 425 430
Pro Gln His Ile Val Leu Pro Glu Ser Val Asp Lys Arg Val Leu Ala
435 440 445
Ala Ala Ala Asp Val Thr Ala Arg Gly Leu Ala Arg Val Thr Leu Leu
450 455 460
Gly Asp Pro Thr Thr Val Gln Gln Glu Pro Pro Pro Ser Pro Ser Thr
465 470 475 480
Pro Ala Ala Met Ala Ala Pro Ala Ser Ala Ser Ser Asp Arg Phe Asp
485 490 495
Lys Tyr Val Asp Met Leu Val Glu Ala Arg Lys Lys Lys Gly Met Thr
500 505 510
Arg Glu Ala Ala Ala Asp Thr Leu His Gly Asp Ile Asn Phe Phe Gly
515 520 525
Thr Met Met Val Ala Ala Gly Asp Ala Asp Gly Met Val Ser Gly Ala
530 535 540
Ile His Thr Thr Ala Ser Thr Ile Arg Pro Ala Leu Gln Met Leu Lys
545 550 555 560
Asn Pro Ala Ser Ser Leu Val Ser Ser Ile Phe Phe Met Cys Leu Pro
565 570 575
Asp Arg Val Leu Val Tyr Gly Asp Cys Ala Val Asn Val Ser Pro Ser
580 585 590
Ala Ala Asp Leu Ala Ala Ile Ala Thr Thr Ser Ala Asp Thr Ala Ala
595 600 605
Ala Phe Gly Ile Glu Pro Arg Val Ala Met Leu Ser Tyr Ser Thr Leu
610 615 620
Gly Ser Gly Ala Gly Pro Asp Val Gln Lys Val Thr Glu Ala Val Ala
625 630 635 640
Leu Val Lys Gln Gln Arg Gln Asp Ile Lys Val Glu Gly Pro Ile Gln
645 650 655
Tyr Asp Ala Ala Ile Asp Pro Ala Val Ala Ala Val Lys Val Lys Gly
660 665 670
Gly Ser Glu Val Ala Gly Arg Ala Thr Val Phe Val Phe Pro Asp Leu
675 680 685
Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln Gln Ser Thr Gly Ala
690 695 700
Ile Ala Met Gly Pro Val Met Gln Val Cys Val Arg Val Ser Ala Gly
705 710 715 720
Val Gly Gly Leu Leu Lys Pro Val Asn Asp Leu Ser Arg Gly Cys Thr
725 730 735
Val Pro Asp Ile Val Asn Thr Ile Cys Val Thr Ser Ile Gln Ala Met
740 745 750
Gln Phe Lys Gln Arg Thr Gln Ala Ala Val Ala Ala Ala Ala Ala Pro
755 760 765
Lys
<210> 42
<211> 2310
<212> DNA
<213> Chlamydomonas reinhardtii
<400> 42
atggctttcg cttcttcttc tatggctgct ttgtctagac ctttggctgc tgtttcttct 60
ggtttgggtt ctgctttatc aagagcctct caattattga cctctggttc tttatcttct 120
tctccaactg cttctcattc ctccactaga agattcattt ctgatggtac tgttggttct 180
aagggtagac cagattcttt gttcttgtcc gatatttctg gtagaccaaa tgctactgct 240
aacgactatt acaacaaggc catggttaag agacaagttt tcgctgatca taaggttgat 300
gttttgggtg ttgttatcaa cggtttgcca agagaacatc atgccatttt gtcatcacaa 360
ttgagagata agttggaaag agctggtttg ccatttgctg gtgctttgcc agaagatcca 420
gttttatctt cagttagacc accacctcca gctttggata agccatctcc aggtttgact 480
tgtgtttctt catgtgctcc atctcataga tatccaccaa ctttgccacc attgggttta 540
ggtttgttgt tgtgtccatg tccagttcca gctagattgc aattgattgc ttctggtaag 600
caatccgaag ttttggatag aatctatgct gcttacatgg cttataaggc tggtggtgaa 660
ttggatttgt gtttggttga aggtccaggt ccattgatgg gtggtactga attagatgct 720
caagttgttg ttggttccca aagattggaa gaattattgg aaaccttggt tgaaagacca 780
atgggtagac ctttagttgt tacttctgct gatagattgg atatcgtctt gggtttgttg 840
gctgctcaat tgtctgttag aggtccatct gttgctggtg ttttattgac tcaagctggt 900
gcatctagaa tcactagatc ttatgctaag tccgccgttg acaatatttt cgctggtttg 960
tctaacaata ctggtgctag tggtggtggt ccagatggtg ctgctgctgc aaatggttct 1020
gctcaaggtt cattatacag aggtgctttg ttgccagttt tgtctactga caaacatttg 1080
gctgaagctt tggctgttat tggtagaatg gatgcttcta ttttgccaac ctccattaga 1140
aaggttaccc aatgtaaagt tgccggtgct gcagttatgt tgttcgataa gtatattgat 1200
gccaacgctg ttgtcaccgg tttacaaaaa tctagaccaa ctagagttac cccaaagatg 1260
tttcaacata ccatgaaggc tatgtgtaga gcttctccac aacatatcgt tttgcctgaa 1320
tctgttgata agagagtttt ggctgcagct gctgatgtta ctgctagagg tttggcaaga 1380
gttactttgt tgggtgatcc aactactgtt caacaagaac ctccaccatc tccatctact 1440
ccagctgcta tggcagctcc agcttctgct tcttcagata gatttgataa gtacgtcgac 1500
atgttggtcg aagccagaaa aaagaaaggt atgactagag aagctgctgc agatacttta 1560
catggtgaca ttaacttttt cggtactatg atggttgctg ccggtgatgc tgatggtatg 1620
gtttctggtg ctattcatac aaccgcttct actattagac cagccttgca aatgttgaaa 1680
aacccagctt cttcattggt ttcttccatt tttttcatgt gtttgccaga cagagtcttg 1740
gtttatggtg attgtgctgt taacgtttct ccatctgcag ctgatttggc agctattgct 1800
actacttcag cagatacagc tgctgctttt ggtattgaac ctagagttgc tatgttgtcc 1860
tattctactt tgggttcagg tgctggtcct gatgttcaaa aagttactga agctgttgcc 1920
ttggtcaagc aacaaagaca agatatcaaa gtcgaaggtc caattcaata cgatgctgct 1980
attgatccag ctgttgcagc tgttaaggtt aagggtggtt ctgaagttgc tggtagagct 2040
actgtttttg ttttcccaga tttgaacacc ggtaacaaca cttacaaagc cgttcaacaa 2100
tctacaggtg ctattgcaat gggtccagtt atgcaagttt gtgttagagt ttcagctggt 2160
gttggtggtt tgttgaagcc agttaatgat ttgtcaagag gttgtaccgt tccagatatc 2220
gttaacacta tttgcgttac ctccattcaa gccatgcaat tcaaacaaag aacacaagct 2280
gctgttgctg ctgccgctgc tccaaagtaa 2310
<210> 43
<211> 801
<212> PRT
<213> Coccomyxa subellipsoidea
<400> 43
Met Leu Pro Arg Ala Leu Arg Ser Ala Lys Ser Ile Phe Ser Ala Val
1 5 10 15
Arg Pro Pro Ala Gly Val His Asp Val Leu Ser Leu Pro Gly Ile Gln
20 25 30
His Val Pro Met Glu Ala Val Gln Arg Gln Ala Leu Pro Cys Leu Leu
35 40 45
Pro Arg Met Met Ser Thr Ser Ile Ser Asp Glu His Gln Pro Ser Lys
50 55 60
Lys Ser Leu Tyr Val Leu Asn Val Glu Gly Lys Arg Thr Leu Gly Pro
65 70 75 80
Leu Leu Ile Gly Leu Met Asp Tyr Phe Glu Arg Trp Leu Pro Asn Val
85 90 95
Gly Phe Phe Gln Pro Ile Gly Gly Glu Pro Phe Pro Asp Ser Asp Ser
100 105 110
Asp Glu Pro Arg His Val Glu Leu Ile Arg Lys Ala Phe Asp Leu Lys
115 120 125
Asp Asp Pro Arg Ser Met Tyr Ala Val His Arg Arg Glu Ala Ile Ser
130 135 140
Leu Leu Ala His Asp Lys Ala Asp Glu Leu Leu Asp Lys Ile Tyr Ser
145 150 155 160
Ser Phe Glu Glu Tyr Gln Ser Arg His Asp Leu Val Val Ile Glu Gly
165 170 175
Thr His Glu Asp Gly Arg Leu Asn Val Pro Gly Asn Arg Leu Glu Leu
180 185 190
Asn Gly Arg Ile Ala Ala Thr Leu Ala Ala Pro Val Leu Met Val Leu
195 200 205
Asp Ala Gly Asp Asp Ile Ser Val Asp Asp Leu Ile Asp Lys Ala Leu
210 215 220
Leu Ser Lys Asn Gly Leu Glu Glu Gln Arg Cys Glu Val Leu Gly Leu
225 230 235 240
Ile Val Asn Lys Ala Pro Gln Lys Glu His Pro Ile Leu Lys Ala Gln
245 250 255
Leu Ser Lys Lys Leu Ala Glu His Ser Leu Pro Leu Val Gly Val Val
260 265 270
Pro His Asp Pro Leu Ile Ser Ser Val Arg Leu Asp Glu Ile Gln Ala
275 280 285
Ala Leu Ser Ala Lys Val Ile Ala Gly Arg Lys Gly Pro His Asp Leu
290 295 300
Thr Val Asp Lys Val Tyr Val Ala Thr Ala Asp Leu Asp Thr Thr Leu
305 310 315 320
Arg Arg Leu Thr Asp His Thr Ser Ser Arg Pro Leu Ile Val Thr Asp
325 330 335
Ile Gly Arg Ser Asp Leu Ile Leu Gly Leu Thr Ser Ala Asn Glu Ser
340 345 350
Thr Ile Gly Pro His Val Thr Gly Ile Leu Cys Thr Asn Ser Glu Tyr
355 360 365
Gly Arg Arg Asp Met Ser Pro His Val His Ala Ile Leu Gln Ala Lys
370 375 380
His Ser Ala Leu Lys Asp Gln Glu Asp Ala Gly Leu Val Ala Phe Phe
385 390 395 400
Pro Val Met Ser Ser Asp Cys Asn Thr Trp Asp Ala Val Thr Ala Val
405 410 415
Ser Arg Ile Gln Pro Ser Ile Arg Pro Thr Ser Lys Ala Lys Ile Gln
420 425 430
Glu Ala Lys Ala Leu Phe Gln Lys Tyr Val Glu Gly Asn Leu Leu Val
435 440 445
Asp Ala Leu Glu Ala Glu Arg Glu Phe Val Met Thr Pro Lys Met Phe
450 455 460
Met His Asn Ile Asn Arg Ile Cys Leu Ser Asn Arg Gln Arg Val Val
465 470 475 480
Leu Pro Glu Ser Asp Asp Ser Arg Val Leu Ala Ala Ala Glu Glu Leu
485 490 495
Thr His Arg Gly Leu Ala Asp Ile Ile Leu Leu Gly Glu Pro Asp Lys
500 505 510
Val Thr Ala Gln Ala Arg Arg Leu Asp Ile Asp Ile Ser Gln Cys Glu
515 520 525
Ile Ile Asp Pro Glu Lys Ser Gly Arg Leu Glu Ala Tyr Ile Glu Lys
530 535 540
Leu Val Glu Val Arg Arg Lys Lys Asn Val Thr Pro Asp Met Ala Arg
545 550 555 560
Asp Phe Leu His Asp Pro Asn Tyr Phe Gly Thr Met Met Thr Leu Cys
565 570 575
Gly Asp Ala Asp Gly Met Val Ser Gly Ala Lys His Thr Thr Ala Ala
580 585 590
Thr Ile Arg Pro Gly Leu Gln Val Leu Arg Thr Lys Asp Ser Pro Leu
595 600 605
Val Ser Ser Val Phe Phe Met Cys Leu Pro Asp Lys Val Leu Ile Tyr
610 615 620
Gly Asp Cys Ala Val Asn Val His Pro Ser Ser Asn Glu Leu Ala Gln
625 630 635 640
Ile Ala Val Thr Ser Ala Asp Thr Ala Ala Ala Phe Gly Val Glu Pro
645 650 655
Arg Val Ala Leu Leu Ser Tyr Ser Thr Phe Gly Ser Gly Ser Gly Pro
660 665 670
Glu Val Asp Arg Val Ala Glu Ala Val Arg Ile Ala Lys Glu Met Arg
675 680 685
Pro Asp Leu Lys Leu Glu Gly Pro Ile Gln Tyr Asp Ala Ala Val Asp
690 695 700
Pro Ala Val Ala Arg Gln Lys Val Lys Gly His Ser Glu Val Ala Gly
705 710 715 720
Lys Ala Thr Val Leu Ile Phe Pro Ser Leu Glu Ala Gly Asn Asn Thr
725 730 735
Tyr Lys Ala Val Gln Gln Ser Thr Gly Ala Ile Ala Ile Gly Pro Ile
740 745 750
Leu Gln Gly Leu Ser Arg Pro Val Asn Asp Leu Ser Arg Gly Cys Thr
755 760 765
Val Val Asp Ile Ile Asn Thr Val Thr Cys Thr Cys Val Gln Ala Val
770 775 780
Ala Ile Lys Asp Arg Glu Lys Ser Pro Ala Pro Glu Ala Ala Pro Ala
785 790 795 800
Ala
<210> 44
<211> 2406
<212> DNA
<213> Coccomyxa subellipsoidea
<400> 44
atgttgccaa gagctttgag atccgctaag tctatttttt ctgctgttag acctccagct 60
ggtgttcatg atgttttgtc tttgccaggt attcaacacg ttccaatgga agctgttcaa 120
agacaagctt tgccatgttt gttaccaaga atgatgtcca cctccatctc tgatgaacat 180
caaccatcca agaagtcctt gtacgttttg aatgttgaag gtaagagaac cttgggtcct 240
ttgttgattg gtttgatgga ttacttcgaa agatggttgc caaacgttgg tttctttcaa 300
cctattggtg gtgaaccatt tccagattct gattcagatg aacctagaca cgttgaattg 360
attagaaagg ccttcgattt gaaggatgac ccaagatcta tgtacgctgt tcatagaaga 420
gaagccattt ctttgttggc tcatgataag gccgatgaat tattggacaa gatctactcc 480
tcattcgaag aataccaatc cagacacgat ttggttgtta tcgaaggtac tcatgaagat 540
ggtagattga atgttccagg taacagattg gaattgaacg gtagaattgc tgctactttg 600
gctgctccag ttttgatggt tttggatgct ggtgatgata tctccgttga tgatttgatt 660
gacaaggctt tgttgtccaa gaacggtttg gaagaacaaa gatgtgaagt cttgggtttg 720
atcgttaaca aggctccaca aaaagaacac ccaattttga aggctcaatt gtctaagaag 780
ttggccgaac attctttgcc attggttggt gttgttccac atgatccatt gatctcttca 840
gttagattgg acgaaattca agctgctttg tccgctaaag ttattgctgg tagaaaaggt 900
cctcatgatt tgaccgttga taaggtttat gttgctactg ctgatttgga taccaccttg 960
agaagattga ctgatcatac ttcttccaga ccattgattg tcaccgatat tggtagatcc 1020
gatttgattt tgggtttaac ctctgctaac gaatccacta ttggtccaca tgttactggt 1080
attttgtgca ccaattctga atacggtaga agagatatgt ccccacatgt tcatgctatc 1140
ttgcaagcta aacattctgc cttgaaggat caagaagatg ctggtttggt tgcttttttc 1200
cctgttatgt catctgattg caatacctgg gatgctgtta ctgctgtttc tagaattcaa 1260
ccatctatca gaccaacctc caaggctaaa attcaagaag ctaaagcttt gttccaaaag 1320
tatgtcgaag gtaacttgtt ggttgatgct ttggaagctg aaagagaatt cgttatgact 1380
ccaaagatgt ttatgcacaa catcaacaga atctgcttgt ccaacagaca aagagttgtt 1440
ttgccagaat ccgatgattc tagagtttta gctgctgctg aagaattgac tcatagaggt 1500
ttggctgata tcatcttgtt gggtgaacca gataaggtta ctgctcaagc tagaagattg 1560
gatatcgaca tttcccaatg cgaaatcatc gatccagaaa agtctggtag attagaagcc 1620
tacatcgaaa agttggtcga agttagaaga aagaagaacg ttactccaga tatggccaga 1680
gatttcttgc atgatccaaa ttacttcggt actatgatga ccttgtgtgg tgatgctgat 1740
ggtatggttt ctggtgctaa acatacaact gctgctacaa ttagaccagg tttacaagtc 1800
ttgagaacta aggattctcc attggtcagt tccgttttct ttatgtgttt gccagacaag 1860
gttttgatct acggtgattg tgctgttaac gttcacccat cttctaatga attggctcaa 1920
attgctgtta cctctgctga tactgcagct gcttttggtg tagaacctag agttgctttg 1980
ttatcctact ctacttttgg ttctggttca ggtccagaag ttgatagagt tgcagaagct 2040
gttagaatcg ctaaagaaat gagaccagac ttgaaattgg aaggtccaat tcaatatgat 2100
gctgctgttg atccagctgt tgctagacaa aaagttaagg gtcattctga agttgctggt 2160
aaggctactg ttttgatttt cccatcttta gaagctggta acaacaccta taaggccgtt 2220
caacaatcta ctggtgctat tgcaattggt ccaatattgc aaggtttgtc cagaccagtt 2280
aacgatttgt ctagaggttg taccgttgtt gacattatta acaccgttac ctgtacctgt 2340
gttcaagcag ttgctattaa ggacagagaa aaatctccag ctccagaagc tgctcctgct 2400
gcttaa 2406
<210> 45
<211> 683
<212> PRT
<213> Guillardia theta
<400> 45
Met Thr Ala Val Glu Glu Val Gly Leu Leu Tyr Ala Val Leu Pro Arg
1 5 10 15
Ala Pro Arg Val Gly Tyr Phe Arg Pro Phe Ala Glu Gly Glu Asn Asp
20 25 30
Arg Ser Ile Ser Leu Met Arg Ser Ile Phe Arg Leu Asp Asp Ser Pro
35 40 45
Glu Val Met Gln Gly Ile Thr Val Ala Glu Ala Thr Lys Leu Leu Ser
50 55 60
His Gly Gln Glu Lys Glu Leu Phe Asp Gln Ile Leu Ser Lys Tyr Val
65 70 75 80
Glu Tyr Arg Lys Gly Lys Asp Phe Val Leu Val Ser Cys Gly Arg Leu
85 90 95
Glu Asn Asp Ser His Phe Trp Ser Gln Lys Met Ala Ala Ala Leu Asn
100 105 110
Leu Pro Val Leu Leu Ile Ser Asp Val Gly His Glu Ser Asp Leu Ala
115 120 125
Ile Ile Lys Gly Gly Leu Glu Ser Ser Asn Val Lys Ile Ala Gly Val
130 135 140
Leu Met Ser Gly Leu Pro Pro Gly Asn Glu Tyr Ala Glu Thr Ala Asn
145 150 155 160
Glu Cys Lys Glu Ala Ile Glu Gln Leu Gly Leu Arg Ser Val Gly Met
165 170 175
Leu Pro Lys Ser Ala Ile Ile His Gln Val Thr Met Ala Glu Val Val
180 185 190
Asp Ala Leu Gly Ala Lys Val Phe Phe Gly Glu Glu Ser Leu Asp Arg
195 200 205
Ser Ile Val Lys Asp Ile Thr Val Ala Thr Leu Asp Met Asn Arg Met
210 215 220
Leu His Arg Leu Arg Val His Pro Gly Thr Leu Val Ile Val His Ser
225 230 235 240
Gly Arg Ala Asp Val Leu Leu Ser Leu Val Leu Ala Ala Arg Ser Ser
245 250 255
Asn Tyr Pro Arg Pro Ala Gly Ile Leu Leu Thr Gly Ser Arg Asn Leu
260 265 270
Asp Pro Asp Val Asp Asp Ile Leu Cys Gly Leu Asn Gly Ile Ala Met
275 280 285
Pro Val Ile Gly Val Glu Glu Asp Thr Phe Ala Cys Thr Thr Thr Leu
290 295 300
Leu Lys His Arg Pro Val Leu Leu Pro Ser Ser Thr Thr Lys Ile Glu
305 310 315 320
Ala Ala Gln Val Leu Phe Gln Lys His Ile Asp Pro Lys Phe Leu Asn
325 330 335
Gln Leu Val Ala Thr Asn Ala Asp Asp Tyr Val Val Thr Pro Lys Leu
340 345 350
Phe Gln His Asn Ile Phe Ser Ala Ala Arg Thr Asp Lys Gln Arg Ile
355 360 365
Val Leu Pro Glu Gly Asp Asp Pro Arg Val Leu Ala Ala Ala Gly Glu
370 375 380
Leu Leu Ala Arg Asn Leu Cys Glu Val Thr Ile Leu Gly Lys Glu Asp
385 390 395 400
Leu Ile Gln Asn Lys Ala Lys Arg Asn His Val Ser Leu Lys Gly Ala
405 410 415
Lys Ile Val Asp Pro Glu Thr Glu Thr Thr Glu Glu Met Val Asp Ala
420 425 430
Leu Tyr Asn Ala Arg Lys Ser Lys Gly Met Thr Lys Glu Leu Ala Arg
435 440 445
Asp Met Leu Gln Gly Glu Pro Asn Trp Phe Gly Thr Met Met Met Tyr
450 455 460
Leu Asp Gln Ala Asp Gly Met Val Ser Gly Ala Cys His Ser Thr Ala
465 470 475 480
Ala Thr Met Arg Pro Ala Leu Gln Val Ile Lys Met Ala Pro Gly Phe
485 490 495
Ser Leu Val Ser Ser Val Phe Phe Met Leu Leu Pro Gly Arg Val Leu
500 505 510
Val Phe Gly Asp Cys Ala Ile Asn Val Asp Pro Thr Ala Asp Glu Leu
515 520 525
Ala Glu Ile Ala Val Ala Ser Ala His Thr Ala Arg Ala Phe Gly Ile
530 535 540
Leu Pro Arg Val Ala Met Leu Ser Tyr Ala Thr Gly Asp Ser Asn Gln
545 550 555 560
Gly Pro Met Ile Asp Lys Val Arg Glu Ala Thr Lys Lys Ala Arg Leu
565 570 575
Leu Ala Pro Asn Glu Leu Ile Glu Gly Pro Ile Gln Phe Asp Ala Ala
580 585 590
Val Asp Pro Ala Val Ala Ala Val Lys Tyr Lys Gly Gln Asp Ser Pro
595 600 605
Val Ala Gly Lys Ala Thr Val Leu Val Phe Pro Asp Leu Asn Ala Gly
610 615 620
Asn Asn Ala Tyr Lys Ala Val Gln Gln Ala Ser Lys Ser Ile Ala Val
625 630 635 640
Gly Pro Ile Met Gln Gly Leu Arg Lys Pro Val Asn Asp Leu Ser Arg
645 650 655
Gly Cys Thr Ile Asp Asp Ile Val Asn Thr Val Val Val Thr Cys Leu
660 665 670
Gln Ser Lys Ala Ser Lys Glu Leu Ala Lys Lys
675 680
<210> 46
<211> 2052
<212> DNA
<213> Guillardia theta
<400> 46
atgaccgctg ttgaagaagt tggtttgttg tacgctgttt tgccaagagc accaagagtt 60
ggttatttta gaccatttgc tgaaggtgaa aacgacagat ccatttcctt gatgagatcc 120
atcttcagat tggatgattc cccagaagtt atgcaaggta ttactgttgc tgaagctacc 180
aagttgttgt ctcatggtca agaaaaagaa ttattcgacc aaatcttgtc caagtacgtc 240
gaatacagaa agggtaagga tttcgttttg gtttcttgcg gtagattgga aaacgattct 300
catttctggt cccaaaaaat ggctgctgct ttgaatttgc cagtcttgtt gatttctgat 360
gtcggtcatg aatctgattt ggccattatt aagggtggtt tggaatcctc caatgttaag 420
attgctggtg ttttgatgtc tggtttgcca ccaggtaatg aatatgctga aactgctaac 480
gaatgcaaag aagccattga acaattgggt ttgagatccg ttggtatgtt gccaaagtct 540
gccattattc atcaagttac catggccgaa gttgttgatg ctttgggtgc taaagttttc 600
ttcggtgaag aatccttgga cagatctatc gttaaggata ttacagttgc caccttggac 660
atgaacagaa tgttgcatag attgagagtt cacccaggta ctttggttat agttcattct 720
ggtagagccg atgtcttgtt gtctttggtt ttggctgcta gatcttctaa ttatccaaga 780
ccagcaggta ttttgttgac cggttctaga aatttggatc cagatgttga tgatatcttg 840
tgcggtttga atggtattgc catgccagtt attggtgtcg aagaagatac tttcgcttgt 900
actactactt tgttgaagca cagaccagtt ttgttgccat cttctactac taagattgaa 960
gctgcccaag tcttgttcca aaaacatatt gatccaaagt tcttgaatca attggttgct 1020
accaacgccg atgattatgt tgttactcca aagttgttcc aacacaacat tttctctgct 1080
gctagaaccg ataagcaaag aatagttttg ccagaaggtg acgatccaag agttttagct 1140
gctgctggtg aattattggc tagaaacttg tgcgaagtta ctatcttggg taaagaagat 1200
ttgattcaaa acaaggccaa gagaaaccac gtttctttga aaggtgctaa gatcgttgat 1260
ccagaaactg aaactactga agaaatggtt gatgccttgt acaacgctag aaaatctaag 1320
ggtatgacca aagaattggc cagagatatg ttgcaaggtg aacctaattg gttcggtact 1380
atgatgatgt atttggacca agctgatggt atggtttctg gtgcttgtca ttctacagct 1440
gctactatga gaccagcttt acaagttatt aagatggctc caggtttctc cttagtctct 1500
tctgtttttt tcatgttgtt gccaggtaga gtcttggttt ttggtgattg tgctatcaat 1560
gttgatccaa ccgctgatga attggctgaa attgctgttg cttctgctca tactgctaga 1620
gcttttggta ttttgcctag agttgctatg ttgtcttatg ctaccggtga ttctaatcaa 1680
ggtccaatga ttgataaggt tagagaagct actaagaagg ccagattatt ggcaccaaac 1740
gaattgattg aaggtcctat tcaattcgat gctgctgttg atcctgctgt tgcagctgtt 1800
aagtacaaag gtcaagattc tccagttgct ggtaaagcta ctgttttagt tttcccagat 1860
ttgaacgctg gtaacaatgc ttacaaagct gttcaacaag cctccaagtc tattgcagtt 1920
ggtccaatca tgcaaggttt gagaaaacca gttaacgact tgtctagagg ttgcactata 1980
gatgatatcg ttaacaccgt tgttgttacc tgcttgcaat ctaaggcttc taaagaatta 2040
gccaagaagt aa 2052
<210> 47
<211> 293
<212> PRT
<213> Helicosporidium sp.
<400> 47
Met Arg Gly Gln Ala Asp Gly Ala Asp Arg Leu Asn Ala Pro Leu Ser
1 5 10 15
Ser Ala Ser Pro Pro Ser Ser Ser Ala Pro Ser Leu His Ser Lys Tyr
20 25 30
Thr Gln Leu Leu Leu Ser Arg Arg Ala His Lys Gly Leu Thr Leu Ala
35 40 45
Ser Ala Leu Asp Gln Met Gln Asp Pro Asn Val Val Gly Thr Leu Met
50 55 60
Val Ala Cys Gly Asp Ala Asp Gly Met Val Ser Gly Ala Thr Cys Thr
65 70 75 80
Thr Ala Ala Thr Ile Arg Pro Ala Leu Gln Leu Leu Lys Ala Pro Gly
85 90 95
Arg Leu Val Ser Ser Leu Phe Phe Met Cys Leu Pro Asp Arg Val Leu
100 105 110
Ala Tyr Gly Asp Cys Ala Val Asn Pro Asp Pro Ser Ala Glu Gln Leu
115 120 125
Ala Gln Ile Ala Glu Ser Ala Ala Glu Thr Thr Arg Ala Phe Gly Val
130 135 140
Glu Pro Arg Val Ala Met Leu Ser Tyr Ser Thr Leu Gly Ser Gly Ser
145 150 155 160
Gly Pro Ala Val Asp Lys Val Ala Ala Ala Val Glu Lys Leu Gln Ala
165 170 175
Gln Arg Pro Asp Leu Met Val Glu Gly Pro Ile Gln Tyr Asp Ala Ala
180 185 190
Val Asp Ser Thr Val Ala Ala Ala Lys Val Lys Lys Ala Ser Glu Val
195 200 205
Ala Gly Arg Ala Thr Val Cys Val Phe Pro Asp Leu Asn Thr Gly Asn
210 215 220
Asn Thr Tyr Lys Ala Val Gln Gln Ser Thr Gly Ala Leu Ala Val Gly
225 230 235 240
Pro Leu Met Met Gly Leu Gln Arg Pro Val Asn Asp Leu Ser Arg Gly
245 250 255
Cys Thr Val Ala Asp Ile Val Asn Thr Ile Ala Cys Thr Ala Val Gln
260 265 270
Ala Ile Gly Leu Lys Asp Ala Asn Ala Ala Ala Glu Glu Lys Lys Ser
275 280 285
Asp Val Tyr Glu Gly
290
<210> 48
<211> 882
<212> DNA
<213> Helicosporidium sp.
<400> 48
atgagaggtc aagctgatgg tgctgataga ttgaatgctc cattgtcaag tgcttctcca 60
ccatcttcat ctgctccatc tttacattct aagtacaccc aattgttgtt gtctagaaga 120
gcacataagg gtttgacttt ggcttctgct ttggatcaaa tgcaagatcc aaatgttgtc 180
ggtactttga tggttgcttg tggtgatgct gatggtatgg tttctggtgc tacttgtact 240
actgctgcta ctattagacc agccttgcaa ttattgaaag ctccaggtag attggtttcc 300
tccttgtttt ttatgtgctt gccagataga gttttggctt atggtgattg tgctgttaat 360
ccagatccat ctgctgaaca attggctcaa attgctgaat ctgctgctga aactactaga 420
gcttttggtg ttgaacctag agttgctatg ttgtcttatt ctactttggg ttctggttct 480
ggtccagctg ttgataaggt tgctgctgct gttgaaaaat tgcaagctca aagaccagat 540
ttgatggtcg aaggtccaat tcaatatgat gctgcagttg attctactgt tgctgcagct 600
aaagttaaga aggcttctga agttgctggt agagctactg tttgtgtttt tccagacttg 660
aacactggta acaacactta caaagccgtt caacaatcta caggtgcttt ggctgttggt 720
ccattgatga tgggtttaca aagacctgtt aacgacttgt ctagaggttg tacagttgct 780
gatatcgtta acactattgc ttgtactgct gttcaagcca ttggtttgaa agatgctaat 840
gctgctgccg aagaaaaaaa gtctgatgtt tacgaaggtt aa 882
<210> 49
<211> 353
<212> PRT
<213> Perkinsus marinus
<400> 49
Met Leu Ser Arg Ser Thr Ala Arg Cys Ala Pro Ala Ala Leu Ala Gly
1 5 10 15
Ile Arg Gln Arg Ala Met Gln Thr Gly Leu Glu Lys Phe Ile Ala Phe
20 25 30
Pro Glu Val Thr Asp Glu Arg Val Ile Pro Ala Val Ala Lys Val Leu
35 40 45
Lys Glu Lys Ile Ala Gln Pro Val Leu Val Gly Asp Arg Glu Ala Ala
50 55 60
Tyr Lys Cys Ala Lys Ala Asn Asn Val Ser Leu Glu Gly Val Arg Ile
65 70 75 80
Ile Asp Pro Ser Leu His Pro Glu Val Val Glu Gln Thr Ala Thr Val
85 90 95
Leu Phe Gln Lys Arg Gln Lys Lys Gly Met Thr Leu Asp Ala Ala Leu
100 105 110
Asp Thr Val Lys Asn Ser Pro Leu Met Met Ala Asp Leu Met Leu Thr
115 120 125
Thr Gly His Val Gln Gly Cys Val Ala Gly Ala Ser His Thr Ser Ala
130 135 140
Asp Val Ala Arg Ala Ala Leu Gln Thr Val Gly Val Lys Lys Gly Leu
145 150 155 160
Lys Thr Ala Ser Ser Phe Phe Ile Ile Ala Lys Asp Asp Lys Thr Phe
165 170 175
Leu Phe Ser Asp Cys Gly Phe Cys Ile Ala Pro Ser Ile Ser Gln Leu
180 185 190
Ala Glu Ile Ala Ile Thr Thr Ala Gln Thr Cys Glu Asp Val Leu Ala
195 200 205
Thr Thr Pro Arg Val Ala Met Leu Ser Phe Ser Thr Phe Gly Ser Ala
210 215 220
Lys His Glu Tyr Val Thr Arg Val Glu Glu Ala Leu Ala Leu Ala Arg
225 230 235 240
Lys Glu Lys Pro Asp Leu Ala Ile Asp Gly Glu Met Gln Val Asp Ala
245 250 255
Ala Ile Val Pro Glu Val Ala Ala Lys Lys Ala Pro Gly Ser Lys Val
260 265 270
Ala Gly His Ala Asn Val Leu Ile Phe Pro Asp Leu Asn Ala Gly Asn
275 280 285
Ile Ala Tyr Lys Val Ala Glu Arg Phe Gly Gly Tyr Gln Ala Val Gly
290 295 300
Pro Ile Phe Gln Gly Leu Ala Tyr Pro Thr Asn Asp Leu Ser Arg Gly
305 310 315 320
Cys His Ala Glu Asp Val Val Asp Ala Ala Ala Val Thr Val Leu Gln
325 330 335
Gly Ser Ser Ile Pro Ile Pro Thr Gly Pro Ala Pro Gly Asp Ile Leu
340 345 350
Asn
<210> 50
<211> 1062
<212> DNA
<213> Perkinsus marinus
<400> 50
atgttgtcta gatctactgc tagatgtgct ccagctgctt tggctggtat tagacaaaga 60
gctatgcaaa ctggtttgga aaagttcatt gctttcccag aagttaccga cgaaagagtt 120
attccagctg ttgctaaggt cttgaaagaa aagattgctc aaccagtttt ggtcggtgat 180
agagaagctg cttacaaatg tgctaaggct aacaacgttt ctttggaagg tgttagaatc 240
atcgatccat cattgcatcc agaagttgtt gaacaaactg ctaccgtttt gttccaaaaa 300
agacaaaaaa agggtatgac cttggatgct gctttagata ctgttaagaa ctccccattg 360
atgatggctg atttgatgtt gactactggt catgttcaag gttgtgttgc tggtgcttct 420
catacttctg ctgatgttgc tagagctgca ttgcaaactg ttggtgttaa gaagggtttg 480
aaaactgcct cctccttctt cattattgcc aaagatgata agaccttctt gttctctgat 540
tgcggtttct gtattgcccc atctatttct caattggccg aaattgctat tactaccgct 600
caaacttgtg aagatgtttt ggctactact ccaagagttg ctatgttgtc tttctctact 660
ttcggttccg ctaaacatga atacgttacc agagttgaag aagctttggc tttggctaga 720
aaagaaaaac cagatttggc cattgacggt gaaatgcaag ttgatgctgc tatagttcct 780
gaagttgctg ctaaaaaagc tccaggttct aaagttgctg gtcatgctaa tgttttgatc 840
ttcccagatt tgaacgctgg taacattgct tataaggttg ctgaaagatt cggtggttat 900
caagctgttg gtccaatttt tcaaggtttg gcttacccaa ctaacgactt gtctagaggt 960
tgtcatgccg aagatgttgt tgatgcagct gctgttactg ttttacaagg ttcctctatt 1020
ccaattccaa ctggtccagc tcctggtgat attttgaact aa 1062
<210> 51
<211> 788
<212> PRT
<213> Physcomitrella patens
<400> 51
Met Leu Arg Ala Val Gly Lys Lys Val Arg Arg Val Leu Arg Glu Glu
1 5 10 15
Leu Leu Ala Gly Leu Gln Gly Arg Gly Leu Gly Arg Ile Tyr Asp Ala
20 25 30
Ser Gly Thr Gly Val Arg Lys His Gly Trp Gly Ile Ser Ser Arg Glu
35 40 45
Thr His Thr Asn Ser Leu Tyr Ile His His Thr Ile Gly Gly Val Gly
50 55 60
Arg Asp Ser Val Ala Val Thr Val Gly Leu Leu His Ser Leu Glu Arg
65 70 75 80
Leu Gln Pro Gly Ile Gly Tyr Phe Arg Pro Ile Asp Gln Thr Thr Ile
85 90 95
Gly Gly Tyr Arg Ser Lys Leu Ile Lys Ser Val Phe Lys Met Lys Asp
100 105 110
Asp Pro Ala Ile Met Gln Gly Val Thr Gln Asp Arg Ala Tyr Glu Leu
115 120 125
Val Thr Asn Asp Lys Ile Asp Asp Leu Leu Glu Glu Val Leu Lys Ala
130 135 140
Tyr Glu Ala Cys Arg Val Lys His Asp Phe Val Val Val Glu Gly Thr
145 150 155 160
Ser Leu Arg Gly Gly Gly Asp Asp Thr Val Thr Leu Asn Ala Lys Ile
165 170 175
Ala Gln Thr Leu Gly Ser Ser Ala Leu Leu Val Thr Asp Ala Gly Ile
180 185 190
Ala Cys Gly Lys Met Asp Lys Met Lys Asn Trp Asp Gly Phe Asp Trp
195 200 205
Glu Lys Arg Val Val Asn Asn Ala Lys Leu Ser Asp Leu Val Phe Arg
210 215 220
Arg Glu His Val Asp Val Val Gly Ala Ile Val His Arg Thr Pro Gln
225 230 235 240
Thr Glu Arg Lys Asp Lys Leu Leu Arg Lys Val Phe Glu Glu Met Lys
245 250 255
Ile Pro Phe Val Gly Ala Leu Pro Glu Asp Ser Val Leu Arg Ser Val
260 265 270
Gln Val Gln Asp Val Ala Lys Lys Leu Glu Ala Gly Leu Leu Tyr Pro
275 280 285
Val Glu Asp Glu Glu Val Ala Met Ser Thr Glu Val Thr Gln Tyr Leu
290 295 300
Val Ala Thr Glu Gln Leu Ser Asp Leu Leu Arg His Leu Pro Arg Tyr
305 310 315 320
Val Asp Pro Thr Lys Gly Ser Ile Val Ile Thr Ser Ala Asn Arg Val
325 330 335
Asp Ile Leu Leu Gly Leu Ile His Leu His Glu Ser Lys Ser Asn Ala
340 345 350
Asn Ile Ala Ala Val Val Leu Ser Gly Gly Lys Pro Pro Pro Arg Glu
355 360 365
Val His Glu Leu Leu Lys Ala Arg Asn Ser Gly Thr Leu Pro Ile Ile
370 375 380
Leu Ser Pro Gln Met Thr Phe Glu Thr Ala Ser Ala Leu Ala Asn Val
385 390 395 400
Glu Gly Tyr Ile Ser Ser Lys Thr Pro Leu Lys Val Glu Arg Ala Gln
405 410 415
Thr Leu Phe Asp Asp Asn Ile Asn Met Lys Leu Ile Lys Asp Ala Met
420 425 430
Phe Gln Glu Arg Pro Val Arg Met Asn Ser Lys Leu Phe Gln His Asn
435 440 445
Leu Phe Thr Arg Ala Lys Gln Cys Ile Gln Thr Ile Val Leu Pro Glu
450 455 460
Gly Glu Glu Pro Arg Thr Leu Gln Ala Ala Gly Thr Val Leu Arg Arg
465 470 475 480
Gly Leu Cys Asn Leu Ile Leu Leu Gly Asp Arg Glu Lys Ile Glu Thr
485 490 495
Leu Ala Lys Gln Phe Arg Val Asp Ile Ser Gln Ala Arg Ile Val Asp
500 505 510
Pro Arg Asp Cys Pro Glu Thr Glu Lys Tyr Ala Arg Tyr Phe Tyr Glu
515 520 525
Ser Arg Lys His Lys Gly Ile Thr Leu Gly Gln Ala His Asn Ile Leu
530 535 540
Ile Gly Asp Val Asn Tyr Phe Gly Thr Cys Met Val Ala Glu Gly Ala
545 550 555 560
Ala Asp Gly Met Val Ser Gly Ala Val His Thr Thr Ala Asn Thr Val
565 570 575
Arg Pro Ala Leu Gln Leu Ile Lys Thr Leu Pro Gly Ile Pro Val Val
580 585 590
Ser Ser Val Phe Phe Met Cys Leu Pro Gly Lys Val Leu Val Tyr Gly
595 600 605
Asp Cys Ala Ile Asn Ser Asp Pro Thr Ser Glu Glu Leu Ala Ala Ile
610 615 620
Ala Ile Ala Ser Ala Asp Thr Ala Ala Ala Phe Gly Ile Lys Pro Arg
625 630 635 640
Val Ala Met Leu Ser Tyr Ala Thr Gly Asp Ser Asn Lys Gly Pro Leu
645 650 655
Ile Gln Lys Val Ile Asp Ala Thr Ala Ile Ala Arg Lys Leu Arg Pro
660 665 670
Asp Leu Leu Ile Glu Gly Pro Leu Gln Tyr Asp Ala Ala Val Asp Pro
675 680 685
Val Ile Ala Lys Thr Lys Met Lys Gly Ala Glu Ser Glu Val Ala Gly
690 695 700
Lys Ala Ser Val Leu Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr
705 710 715 720
Tyr Lys Ala Val Gln Gln Thr Thr Gly Ala Val Ala Met Gly Pro Leu
725 730 735
Leu Gln Gly Leu Arg Lys Pro Val Asn Asp Leu Ser Arg Gly Cys Thr
740 745 750
Val Pro Asp Ile Val Thr Thr Ile Ala Leu Thr Ala Val Gln Ala Ala
755 760 765
Ala Met Lys Glu Thr Ala Lys Arg Glu Thr Pro Lys Glu Asn Leu Val
770 775 780
Ala Ser Ala Ala
785
<210> 52
<211> 2367
<212> DNA
<213> Physcomitrella patens
<400> 52
atgttgagag ctgttggtaa gaaggttaga agagtcttga gagaagaatt attggccggt 60
ttacaaggta gaggtttggg tagaatctat gatgcttctg gtactggtgt tagaaaacat 120
ggttggggta tttcttctag agaaacccat accaactcct tgtacattca tcatacaatt 180
ggtggtgttg gtagagattc tgttgctgtt actgttggtt tgttgcactc tttggaaaga 240
ttacaaccag gtatcggtta cttcagacca attgatcaaa ctaccattgg tggttacaga 300
tccaagttga ttaagtccgt tttcaagatg aaggatgatc cagctattat gcaaggtgtt 360
actcaagata gagcctacga attggttacc aacgataaga tcgatgattt gttggaagaa 420
gtcttgaagg cttacgaagc ttgtagagtt aagcacgatt tcgttgttgt tgaaggtact 480
tctttgagag gtggtggtga tgatactgtt actttgaatg ctaagattgc ccaaaccttg 540
ggttcttctg ctttgttggt tactgatgct ggtattgctt gtggtaagat ggataagatg 600
aagaactggg atggtttcga ttgggaaaaa agagttgtca acaacgccaa gttgtccgat 660
ttggttttta gaagagaaca cgttgatgtt gttggtgcca tagttcatag aactccacaa 720
actgaaagaa aggacaagtt gttgagaaag gttttcgaag aaatgaagat cccattcgtt 780
ggtgctttgc cagaagattc agttttgaga tctgttcaag ttcaagatgt cgccaaaaaa 840
ttggaagcag gtttgttgta tccagttgaa gatgaagaag ttgccatgtc tactgaagtt 900
acccaatatt tggttgccac cgaacaattg tcagatttgt tgagacattt gccaagatac 960
gttgatccaa ctaagggttc tatcgttatt acctctgcta acagagttga catcttgttg 1020
ggtttgattc acttgcacga atctaagtcc aacgctaata ttgctgctgt tgttttgtct 1080
ggtggtaaac caccaccaag agaagttcat gaattattga aggctagaaa ctccggtact 1140
ttgccaatta tcttgtctcc acaaatgact ttcgaaactg cttcagcttt ggctaacgtt 1200
gaaggttaca tttcctctaa gactccattg aaagttgaaa gagcccaaac tttgttcgac 1260
gataacatca acatgaagtt gatcaaggat gccatgttcc aagaaagacc agttagaatg 1320
aactccaagt tgttccaaca taacttgttc accagagcca agcaatgtat tcaaactatt 1380
gtcttgcctg aaggtgaaga acctagaact ttacaagctg ctggtactgt tttgagaaga 1440
ggtttgtgta acttgatttt gttgggtgac agagaaaaga ttgaaacctt ggctaagcaa 1500
ttcagagtcg atatttctca agccagaatt gtcgatccaa gagattgtcc agaaacagaa 1560
aagtacgcta gatacttcta cgaatccaga aagcacaagg gtattacttt gggtcaagcc 1620
cataacattt tgatcggtga tgttaactac ttcggtactt gtatggttgc tgaaggtgct 1680
gctgatggta tggtttcagg tgctgttcat acaactgcta atactgttag accagccttg 1740
caattgatta agactttgcc aggtattcca gttgtctcct ctgttttttt catgtgtttg 1800
cctggtaagg ttttggttta tggtgattgc gctattaact ctgatccaac ctctgaagaa 1860
ttggctgcta ttgctattgc atctgctgat actgctgctg cttttggtat taagccaaga 1920
gttgctatgt tgtcttatgc tactggtgat tctaacaagg gtccattgat tcaaaaggtt 1980
attgatgcta ctgccattgc cagaaagtta agaccagact tgttaatcga aggtccattg 2040
caatatgatg ctgcagttga tccagttatt gctaagacaa aaatgaaggg tgccgaatct 2100
gaagttgctg gtaaagcttc tgttttgatc ttcccagatt tgaacactgg taacaacact 2160
tacaaggctg ttcaacaaac tactggtgct gttgctatgg gtccattatt gcaaggtttg 2220
agaaagccag tcaacgattt gtctagaggt tgtactgttc cagatatcgt taccactatt 2280
gctttgactg ctgttcaagc tgccgctatg aaggaaactg ctaaaagaga aactccaaaa 2340
gaaaacttgg tagcttctgc tgcttga 2367
<210> 53
<211> 761
<212> PRT
<213> Phytophthora infestans
<400> 53
Met Trp Thr Leu Arg Asn Thr Phe Arg Arg Thr Ser Val Ala Leu Pro
1 5 10 15
Ser Gln Arg Arg Ala Leu Thr Ala Ala Ala Ile Ala Glu Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Phe Val Thr Ser Thr Glu Val Thr Glu Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Thr Asn Thr Leu Glu Gln Lys Phe Ala
50 55 60
Arg Val Gly Tyr Phe Arg Pro Ile Gln Pro Ile Val Glu Thr Asp His
65 70 75 80
His Val Asp Val Met Lys Gln Gln Leu Gly Leu Thr Lys Ser Val Glu
85 90 95
Gln Leu Tyr Gly Val Thr Ser Glu Arg Ala Ile Glu Tyr Trp Leu Asn
100 105 110
Gly Lys Gly Asp Asp Leu Val Glu Glu Ile Leu Asp Arg Tyr Glu Ala
115 120 125
Cys Arg Glu Gly His Asp Phe Met Ile Ile Glu Gly Ser Gln Leu Ser
130 135 140
Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp Ile Ala Lys
145 150 155 160
Ala Ile Gly Ser Pro Val Leu Thr Ile Ser Asp Phe Ser Glu Ser Ala
165 170 175
Asn Thr Asn Ser Asp Leu Leu Glu Glu Ile Leu Ser Arg Thr Ala Phe
180 185 190
Asn Lys Asp Gln Val Glu Gly Ala Gly Leu Asn Phe Ile Gly Asn Ile
195 200 205
Ala Asn Arg Val Asn Thr Lys Asp Pro Lys Ala Leu Arg Glu Ala Ile
210 215 220
Arg Ala Lys Leu Arg Glu Lys Asp Leu Pro Phe Leu Gly Phe Leu Pro
225 230 235 240
Lys Asp Asp Phe Ile Ala Ser Lys Arg Leu Asn Glu Val Thr His Gln
245 250 255
Leu Gly Ala Lys Gln Leu Phe Gly Thr Lys Ala Ile Pro Asn Asn Val
260 265 270
Val Val Thr Ser Ala Val Val Ala Thr Ser Ala Leu Lys Asp Leu Phe
275 280 285
Ala His Leu Lys Asn Tyr Lys Asp Gly Ala Leu Val Ile Thr Ser Ala
290 295 300
Asp Arg Ser Asp Val Met Leu Gly Leu Met Ala Ser Arg Leu Pro Gly
305 310 315 320
Ile Leu Pro Asn Val Ser Ala Ile Val Leu Thr Asn Gly Ser Tyr Pro
325 330 335
His Ser Asn Thr Gln Glu Ile Leu Gln Gly Val Glu Ala Leu Asp Lys
340 345 350
Thr Gly Leu Ser Ile Pro Ile Phe Ser Val Pro Glu Asp Thr Phe Thr
355 360 365
Thr Ala Asp Lys Phe Ser Lys Val Ser Thr Asp Ile Leu Pro Thr Ser
370 375 380
Gln Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe Val Gly
385 390 395 400
Lys Glu Gly Ile Ile Gly Glu Leu Asp Glu Gly Met Val Val Asn Arg
405 410 415
Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg Ala Val
420 425 430
Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val Leu Gln
435 440 445
Ala Ala Asp Gln Val Leu Arg Gln Lys Leu Ser Lys Val Thr Ile Leu
450 455 460
Gly Asn Pro Asp Asp Ile Glu Arg His Ala Lys Ser Leu Thr Leu Asp
465 470 475 480
Leu Ser Arg Ala Asn Ile Val Arg Thr Ala Asp Ser Glu Leu Leu Asp
485 490 495
Lys Tyr Val Asp Gln Tyr Phe Glu Lys Arg Lys His Lys Gly Val Thr
500 505 510
Arg Glu Ser Ala Arg Asp Ala Val Leu Glu Glu Thr Cys Phe Gly Thr
515 520 525
Met Met Val Glu Met Gly Asp Ala Asp Gly Met Val Ser Gly Ala Cys
530 535 540
His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile Lys Thr
545 550 555 560
Thr Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys Leu Glu
565 570 575
Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp Pro Ser
580 585 590
Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser Ala Glu
595 600 605
Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala Thr Gly
610 615 620
Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala Thr Lys
625 630 635 640
Met Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro Ile Gln
645 650 655
Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu Lys Ala
660 665 670
Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn Val Leu Ile Phe
675 680 685
Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln Gln Ser
690 695 700
Thr Gly Cys Ile Ala Met Gly Pro Met Leu Gln Gly Leu Arg Lys Pro
705 710 715 720
Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp Ile Val Thr Thr
725 730 735
Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile Leu Lys Arg Glu
740 745 750
Ala Lys Ala Lys Val Glu Ala Ala Ala
755 760
<210> 54
<211> 2286
<212> DNA
<213> Phytophthora infestans
<400> 54
atgtggactt tgagaaatac cttcagaaga acctctgttg ctttgccatc tcaaagaaga 60
gctttgactg ctgctgctat tgctgaaggt aaagttccaa tcaacaactt gttcgttacc 120
tctactgaag ttactgaaaa gactgcccca gttttgattg gtttgactaa cactttggaa 180
caaaagttcg ctagagtcgg ttacttcaga ccaattcaac ctatcgttga aaccgatcat 240
cacgttgatg ttatgaagca acaattgggt ttgaccaagt ccgtcgaaca attatatggt 300
gttacttccg aaagagccat cgaatattgg ttgaatggta agggtgatga cttggtcgaa 360
gaaatcttgg atagatacga agcttgtaga gaaggtcacg atttcatgat tatcgaaggt 420
tcccaattgt ccaaacatga atctgctatg tcctggaaga ttaacgttga tattgctaag 480
gctatcggtt ctccagtctt gactatttct gatttttctg aatccgccaa caccaactct 540
gatttgttgg aagaaatttt gtccagaacc gccttcaaca aggatcaagt tgaaggtgct 600
ggtttgaact tcattggtaa cattgctaac agagtcaaca ccaaagatcc aaaggctttg 660
agagaagcta ttagagctaa gttgagagaa aaggacttgc catttttggg tttcttgcca 720
aaggatgatt tcattgcctc taagagattg aacgaagtta cccatcaatt gggtgccaag 780
caattatttg gtacaaaggc tattccaaac aacgtcgttg taacttctgc tgttgttgct 840
acttctgctt tgaaggattt gttcgctcat ttgaagaact acaaggatgg tgctttggtt 900
attacctctg ctgatagatc agatgtcatg ttgggtttaa tggcttctag attgccaggt 960
attttgccaa atgtttccgc tatcgttttg accaatggtt cttacccaca ttctaacacc 1020
caagaaatat tgcaaggtgt tgaagctttg gataagaccg gtttgtctat tccaattttc 1080
tccgttccag aagatacttt cactaccgct gataagttct ctaaggtttc cactgatatc 1140
ttgccaacct cccaattgaa gatcgataga tccaaacaat tattcgatga attcgttggt 1200
aaagaaggta tcatcggtga attggatgaa ggtatggttg ttaacagatc cccaaagcaa 1260
ttccaacact tcttgttctc taaatccaga gccgttcaaa gacatattgt cttgactgaa 1320
ggtgaagata ttagagtctt gcaagctgct gatcaagtct tgagacaaaa gttgtccaag 1380
gttaccattt tgggtaaccc agatgatatt gaaagacacg ctaagtcttt gaccttggat 1440
ttgtctagag ctaacatcgt tagaaccgcc gattctgaat tattggataa gtacgttgac 1500
caatacttcg aaaaaagaaa gcacaagggt gtcactagag aatcagctag agatgctgtt 1560
ttagaagaaa cctgtttcgg tactatgatg gttgaaatgg gtgatgctga tggtatggtt 1620
tctggtgctt gtcatacaac tgctaacact attagaccag ccttgcaatt gattaagact 1680
actccaaata gaccaattgt ctcctccatt ttcttcatgt gtttggaaga tggtgtcaga 1740
atctatggtg attgtgctgt taatactgat ccatccgctc aagatttggc tcaaattgct 1800
gttacatctg ctgaatcagc tgaagctttt ggtttgattc caaaggttgc tttgttgtct 1860
tatgctaccg gtgattctaa ttccggtcca atcattgata aggttagaga agcaactaag 1920
atggcccaag aattgagacc agatttggat atctatggtc caattcaata cgatgctgcc 1980
gttgatgaat ccattgctaa aactaagttg aaagccattc catccggtgc taaagttggt 2040
ggtcaagcta atgttttgat cttcccagat ttgaacactg gtaacaacac ttacaaggcc 2100
gttcaacaat ctactggttg tattgctatg ggtccaatgt tgcaaggttt aagaaagcca 2160
gttaacgact tatctagagg tgctactgtt aaggatatcg ttactactgt tgctattacc 2220
gctattcaag ccgatcaagt tatcttgaaa agagaagcca aagctaaggt tgaagctgct 2280
gcttaa 2286
<210> 55
<211> 760
<212> PRT
<213> Phytophthora parasitica
<400> 55
Met Trp Thr Leu Arg Arg Ser Leu Arg Arg Ser Thr Gly Val Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Thr
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ala Asn Asp Ala Leu Ile Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Asp Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Ser Asn Val Asp Ser Leu Arg
210 215 220
Ala Ser Leu Lys Arg Lys Met Gly Asp Lys Asp Ile Pro Phe Leu Gly
225 230 235 240
Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu Val
245 250 255
Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly His Ser Ile Ala Asn
260 265 270
Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile Thr
290 295 300
Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg Leu
305 310 315 320
Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly Asn
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Gln Ala Leu
340 345 350
Asp Lys Thr Ala Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp Thr
355 360 365
Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile Leu Pro
370 375 380
Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Lys Ile Thr
450 455 460
Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn Ala Lys Met Ala Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Val Ser Pro Ala Asn Ser Ala Leu
485 490 495
Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys Arg Lys His Lys Gly
500 505 510
Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr Phe
515 520 525
Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys
565 570 575
Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu
660 665 670
Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn Val Leu
675 680 685
Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln
690 695 700
Gln Ser Thr Gly Cys Ile Ala Met Gly Pro Met Leu Gln Gly Leu Arg
705 710 715 720
Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp Ile Val
725 730 735
Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile Met Lys
740 745 750
Arg Glu Ala Glu Asn Ala Thr Lys
755 760
<210> 56
<211> 2283
<212> DNA
<213> Phytophthora parasitica
<400> 56
atgtggacct tgagaagaag tttgagaaga tctactggtg ttgctttgcc acatagaaga 60
gctttgactg ctgctgctat ttcacaaggt aaagttccaa tcaacaactt gtacgttacc 120
tctactgaag tcactaagaa aactgcccca gttttgattg gtttggctca tgttttggaa 180
caaaagttca ctaaggtcgg ttacttcaga ccaattcaac catctccaga ttcttctatg 240
gctgatcatc atgttgatgt catgaagcaa caattggaat tgtccaagga cgtcgaagaa 300
ttatacggtg ttacttcttc tagagctatg gaagctatgt tgactggtaa aggtgatgat 360
gttgtcgaag aaatcttgga aagatacgaa caatgcagaa agggtcacga tttcatgatt 420
atcgaaggtt cccaaatctc caaacatgaa tctgctatgt cctggaagat taacgttgat 480
attgctaagg ctatcggttc tcctgttttg atggttactg atttcggtga tacttctgct 540
gctaacgatg ctttgattga agaaatggtt tccagaaccg ttatgggtag agatcaagct 600
gaagatgctg gtttgaatta cttgggtact attgccaata gagtcagagc ctctaatgtt 660
gattctttga gagcttcctt gaagagaaag atgggtgata aggatattcc attcttgggt 720
tttttgccaa tggacgaaat tatcgcctct aagagattga acgaagttac ccatcaattg 780
ggtgccactc aattattcgg tcattccatt gctaatgatg ccgttgttac atctgctgtt 840
gttgctgctt ctgctttgaa agatttgttc gcccatttga aaaagtacaa ggatggtgcc 900
atgatcatta cctctggtga tagatctgat ttgatgttgg gtttgatggt cagtagattg 960
ccaggtgttt tgccaaatat ttccgctatc gttttgacca atggtaacta cccacattct 1020
aacacccaag aaattttgaa gggtgttcaa gctttggata agaccgcttt gtctttgcca 1080
attttctcta ctccaaacga tactttctct accgctgatg gttttgctaa ggtttctact 1140
gatatcttgc catcctccaa gttgaagatc gatagatcca agcaattatt tgatgaattc 1200
gttgaaaaag aaatgttgat cggtgaattg gacgaaggta tggttgttaa tagatcccca 1260
aagcaattcc aacacttctt gttctctaag tccagagctg ttcaaagaca tattgtcttg 1320
actgaaggtg aagatattag agtcttgcaa gctgccgatc aaatcttgag acaaaacttg 1380
tccaagatca ccattttggg tgatccagac gaaattttgt tgaacgctaa gatggctaac 1440
ttggatttgt ctagagccaa tatcgtttct ccagctaatt ctgccttgtt ggataagtac 1500
gttgattact tctacgccaa gagaaaacat aagggtgtca caaaagaatt ggccagagat 1560
tactgtaagg acgaaactta ctttggtaca ttgatggtcg aattaggtga tgctgatggt 1620
atggtttctg gtgcttgtca tacaactgct aacactatta gaccagcctt gcaattgatt 1680
aagactgctc caaatagacc aatcgtttcc tccattttct tcatgtgctt ggaagatggt 1740
gtcagaatct atggtgattg tgctgttaat actgatccat ctgcacaaga tttggctcaa 1800
attgctgtta ctagtgctga atcagctgaa gcttttggtt tgattccaaa ggttgctttg 1860
ttgtcttatg ctaccggtga ttctaattcc ggtccaatca ttgataaggt tagagaagct 1920
accaagatcg cccaagaatt gagaccagat ttggatatct atggtccaat ccaatatgat 1980
gctgccgttg atgaatctat cgctaagaca aaattgaagg ccattccatc tggtgctaaa 2040
gttggtggtc aagctaatgt tttgatcttc ccagatttga acactggtaa caacacttac 2100
aaggctgttc aacaatctac cggttgtatt gctatgggtc caatgttgca aggtttgaga 2160
aaaccagtta acgacttatc tagaggtgct accgttaagg atatcgttac tactgttgct 2220
attaccgcta ttcaagccga tcaagttatc atgaagagag aagctgaaaa cgctaccaag 2280
tga 2283
<210> 57
<211> 762
<212> PRT
<213> Phytophthora ramorum
<400> 57
Met Trp Thr Leu Arg His Ser Val His Arg Ala Pro Thr Ala Leu Pro
1 5 10 15
Gln Arg Arg Ala Leu Thr Ala Ala Ala Ile Ala Gln Gly Lys Val Pro
20 25 30
Ile Asp Asn Leu Phe Val Thr Ser Thr Glu Val Thr Lys Lys Thr Ala
35 40 45
Pro Val Leu Val Gly Leu Ala Asn Thr Leu Glu Gln Lys Phe Ala Lys
50 55 60
Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Ala Gln Ser Ser Met Ala
65 70 75 80
Asp His His Val Glu Val Met Arg Glu Gln Leu Gly Leu Thr Lys Glu
85 90 95
Val His Glu Leu Phe Gly Val Ser Ser Glu Arg Ala Ile Glu Ser Trp
100 105 110
Leu Ser Gly Lys Glu Asp Asp Leu Val Glu Glu Ile Leu Asp Arg Phe
115 120 125
Glu Gln Cys Arg Glu Gly His Asp Phe Met Ile Ile Glu Gly Ser Gln
130 135 140
Leu Ser Lys His Glu Ser Ala Met Ser Trp Lys Val Asn Val Asp Ile
145 150 155 160
Ala Lys Ala Ile Gly Ser Pro Val Leu Thr Ile Ser Asp Phe Ser Glu
165 170 175
Ser Thr His Ser Asn Gly Glu Leu Leu Glu Glu Ile Leu Ser Arg Thr
180 185 190
Ala Leu Asn Lys Asp Gln Val Glu Gly Ala Gly Leu Asn Phe Ile Gly
195 200 205
Asn Ile Ala Asn Arg Val Asn Thr Lys Asp Pro Lys Ala Leu Arg Asp
210 215 220
Ala Leu Arg Ser Lys Leu Asn Glu Lys Asp Leu Pro Phe Leu Gly Phe
225 230 235 240
Leu Pro Lys Asp Asp Phe Ile Ala Ser Lys Arg Leu Asn Glu Val Thr
245 250 255
His Gln Leu Gly Ala Thr Gln Leu Phe Gly Thr Lys Ala Ile Pro Asn
260 265 270
Asn Val Val Val Thr Ser Ala Val Val Ala Thr Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ser His Leu Lys Asn Tyr Lys Asp Gly Ala Leu Val Ile Thr
290 295 300
Ser Ala Asp Arg Ser Asp Ile Met Leu Gly Leu Met Ala Ser Arg Leu
305 310 315 320
Pro Gly Ile Leu Pro Asn Val Ser Ala Ile Val Leu Thr Asn Gly Ser
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Glu Ala Leu
340 345 350
Asp Lys Thr Gly Leu Ser Ile Pro Ile Phe Ser Val Pro Glu Asp Thr
355 360 365
Phe Thr Thr Ala Asp Lys Phe Ser Lys Val Ser Thr Asp Ile Leu Pro
370 375 380
Thr Ser Gln Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Gly Lys Glu Asn Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Val Leu Arg Gln Lys Leu Ser Lys Val Thr
450 455 460
Ile Leu Gly Asn Pro Asp Asp Ile Gln Arg His Ala Lys Ser Leu Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Val Arg Thr Ala Glu Ser Asp Leu
485 490 495
Leu Glu Lys Tyr Val Asp Gln Tyr Phe Glu Lys Arg Lys His Lys Gly
500 505 510
Val Thr Arg Glu Thr Ala Arg Asp Ala Val Leu Glu Glu Thr Cys Phe
515 520 525
Gly Thr Met Met Val Glu Met Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Thr Pro Asn Arg Pro Ile Val Ser Ser Val Phe Phe Met Cys
565 570 575
Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Ala Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Val Asp Ala Ser Ile Ala Lys Thr Lys Leu
660 665 670
Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn Val Leu
675 680 685
Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln
690 695 700
Gln Ser Thr Gly Cys Val Ala Met Gly Pro Met Leu Gln Gly Leu Arg
705 710 715 720
Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp Ile Val
725 730 735
Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile Leu Lys
740 745 750
Arg Glu Ala Gly Ala Ala Ala Ala Lys Leu
755 760
<210> 58
<211> 2289
<212> DNA
<213> Phytophthora ramorum
<400> 58
atgtggacct tgagacattc tgttcataga gcaccaactg ctttgccaca aagaagagct 60
ttgactgctg ctgctattgc tcaaggtaaa gttccaattg ataacttgtt cgtcacctct 120
actgaagtca ctaagaaaac tgctccagtt ttggttggtt tggctaacac tttggaacaa 180
aagtttgcta aggtcggtta cttcagacca attcaaccat ctgctcaatc ttctatggct 240
gatcatcatg ttgaagttat gagagaacaa ttgggtttga ccaaagaagt ccacgaatta 300
ttcggtgtct cttccgaaag agctattgaa tcttggttgt ccggtaaaga agatgacttg 360
gttgaagaaa tcttggacag attcgaacaa tgcagagaag gtcatgattt catgatcatc 420
gaaggttccc aattgtctaa acatgaatct gctatgtcct ggaaggttaa cgttgatatt 480
gctaaggcta ttggttcccc agttttgacc atttctgatt tctctgaatc cacccattcc 540
aacggtgaat tattggaaga aattttgtcc agaaccgcct tgaacaagga tcaagttgaa 600
ggtgctggtt tgaacttcat tggtaacatt gctaacagag tcaacaccaa agatccaaag 660
gctttgagag atgctttgag atctaagttg aacgaaaagg acttgccatt cttgggtttt 720
ttgccaaagg atgacttcat tgcctctaag agattgaacg aagttaccca tcaattgggt 780
gccactcaat tatttggtac aaaggctatt ccaaacaacg tcgttgtaac ttctgctgtt 840
gttgctactt ctgctttgaa ggatttgttc tcccatttga agaactacaa ggatggtgct 900
ttggttatta cctctgctga tagatccgat atcatgttgg gtttaatggc ttctagattg 960
ccaggtatct tgccaaatgt ttctgctatc gttttgacta acggttccta cccacattct 1020
aacacccaag aaatattgaa gggtgttgaa gctttggata agaccggttt gtctattcca 1080
attttctccg ttccagaaga tactttcact accgctgata agttctctaa ggtttccact 1140
gatattttgc caacctctca attgaagatc gacagatcca agcaattatt cgatgaattc 1200
gttggtaaag aaaacttgat cggtgaattg gatgaaggta tggttgttaa cagatcccca 1260
aagcaattcc aacacttctt gttctctaaa tccagagccg ttcaaagaca tattgtcttg 1320
actgaaggtg aagatatcag agttttacaa gctgctgatc aagtcttgag acaaaagttg 1380
tccaaggtta ccattttggg taacccagat gatatccaaa gacatgccaa gtctttgaat 1440
ttggatttgt ctagagccaa catcgttaga actgctgaat ctgatttgtt ggaaaagtat 1500
gtcgaccaat acttcgaaaa aagaaagcac aagggtgtta ctagagaaac tgctagagat 1560
gctgttttag aagaaacctg tttcggtact atgatggtcg aaatgggtga tgctgatggt 1620
atggtttctg gtgcttgtca tacaactgct aacactatta gaccagcctt gcaattgatt 1680
aagactactc caaatagacc aatcgtttcc tccgttttct tcatgtgttt ggaagatggt 1740
gtcagaatct atggtgattg tgctgttaat actgatccat ctgctgctga tttggctcaa 1800
attgctgtta catctgcaga atctgctgaa gcttttggtt tgattccaaa ggttgctttg 1860
ttgtcttatg ctaccggtga ttctaattcc ggtccaatca ttgataaggt tagagaagct 1920
accaagatcg cccaagaatt gagaccagat ttggacatct atggtccaat tcaatatgat 1980
gctgctgttg atgcctccat tgctaagaca aaattgaaag ctattccatc cggtgctaaa 2040
gttggtggtc aagctaatgt tttgatcttc ccagatttga acactggtaa caacacttac 2100
aaggccgttc aacaatctac tggttgtgtt gctatgggtc caatgttgca aggtttgaga 2160
aaaccagtta acgacttatc tagaggtgct accgttaagg atatcgttac tactgttgct 2220
attaccgcta ttcaagccga ccaagttatt ttgaaaagag aagctggtgc tgctgcagct 2280
aagttgtaa 2289
<210> 59
<211> 764
<212> PRT
<213> Phytophthora sojae
<400> 59
Met Trp Thr Leu Arg Arg Ser Leu Arg Arg Ser Pro Val Ser Leu Met
1 5 10 15
Leu Pro Ala His Arg Arg Ala Leu Thr Ala Ala Ala Ala Ser Gln Gly
20 25 30
Lys Val Pro Ile Glu Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys
35 40 45
Lys Thr Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu His Lys
50 55 60
Phe Asp Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser
65 70 75 80
Ser Met Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu
85 90 95
Pro His Asn Val Glu Gln Leu Tyr Gly Val Thr Ser Gln Arg Ala Met
100 105 110
Glu Ala Met Leu Asn Gly Lys Gly Asp Asp Ile Val Glu Glu Ile Leu
115 120 125
Glu Arg Tyr Glu Glu Cys Arg Lys Gly His Asp Phe Met Leu Ile Glu
130 135 140
Gly Ser Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn
145 150 155 160
Val Asp Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp
165 170 175
Phe Gly Asp Ser Ala Ala Lys Asn Gly Glu Leu Leu Glu Glu Met Val
180 185 190
Ser Arg Thr Val Met Gly Lys Asp Gln Ala Asp Ala Ala Gly Leu Asn
195 200 205
Tyr Leu Gly Thr Ile Ala Asn Arg Val Arg Ala Lys Asp Ala Asp Lys
210 215 220
Leu Arg Ala Asp Leu Lys Glu Lys Leu Asp Glu Lys Asp Ile Pro Phe
225 230 235 240
Leu Gly Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn
245 250 255
Glu Val Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly Asn Thr Ile
260 265 270
Ala Asn Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu
275 280 285
Lys Asp Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile
290 295 300
Ile Thr Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser
305 310 315 320
Arg Leu Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn
325 330 335
Gly Asn Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Glu
340 345 350
Ala Leu Asp Lys Thr Gly Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn
355 360 365
Asp Thr Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile
370 375 380
Leu Pro Thr Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp
385 390 395 400
Glu Phe Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Gln Gly Met
405 410 415
Val Val Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys
420 425 430
Ser Arg Ala Val Gln Arg His Ile Val Leu Ser Glu Gly Glu Asp Ile
435 440 445
Arg Val Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Lys
450 455 460
Ile Thr Ile Leu Gly Asn Pro Asp Glu Ile Leu Leu Asn Ala Lys Thr
465 470 475 480
Ala Asn Leu Asp Leu Ser Arg Ala Asn Ile Val Arg Pro Ser Asp Ser
485 490 495
Glu Leu Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Glu Lys Arg Lys His
500 505 510
Lys Gly Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr
515 520 525
Tyr Phe Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val
530 535 540
Ser Gly Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln
545 550 555 560
Leu Ile Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser Val Phe Phe
565 570 575
Met Cys Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn
580 585 590
Thr Asp Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala
595 600 605
Glu Ser Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser
610 615 620
Tyr Ala Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg
625 630 635 640
Glu Ala Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr
645 650 655
Gly Pro Ile Gln Tyr Asp Ala Ala Val Asp Ala Ser Ile Ala Lys Thr
660 665 670
Lys Leu Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn
675 680 685
Val Leu Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala
690 695 700
Val Gln Gln Ser Thr Gly Cys Ile Ala Met Gly Pro Met Leu Gln Gly
705 710 715 720
Leu Arg Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp
725 730 735
Ile Val Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile
740 745 750
Leu Lys Arg Glu Ala Glu Ala Ala Thr Ala Lys Leu
755 760
<210> 60
<211> 2295
<212> DNA
<213> Phytophthora sojae
<400> 60
atgtggacct tgagaagatc tttaagaaga tccccagtct ctttgatgtt gccagctcat 60
agaagagctt tgacagctgc tgctgcttca caaggtaaag ttccaattga aaacttgtac 120
gtcacctcta ctgaagtcac taagaaaact gctccagttt tgattggttt ggcccatgtt 180
ttggaacaca agtttgataa ggttggttac ttcagaccaa tccaaccatc tccagattct 240
tcaatggctg atcatcatgt tgatgtcatg aagcaacaat tggaattgcc acacaacgtc 300
gaacaattat acggtgttac ttctcaaaga gctatggaag ctatgttgaa tggtaagggt 360
gatgatatcg tcgaagaaat cttggaaaga tacgaagaat gcagaaaggg tcacgacttt 420
atgttgattg aaggttccca aatctccaag cacgaatctg ctatgtcttg gaagattaac 480
gttgatattg ctaaggccat tggttctcca gtcttaatgg ttactgattt cggtgattct 540
gctgctaaga acggtgaatt attggaagaa atggtttcca gaaccgttat gggtaaagat 600
caagctgatg ctgctggttt gaattacttg ggtactattg ctaacagagt tagagctaag 660
gatgccgata agttgagagc tgatttgaaa gaaaagttgg acgaaaagga catcccattc 720
ttgggttttt tgccaatgga cgaaattatc gcctctaaga gattgaacga agttacccat 780
caattgggtg ccactcaatt attcggtaat accattgcta acgatgccgt tgttacatct 840
gctgttgttg ctgcttctgc tttgaaagat ttgttcgccc atttgaaaaa gtacaaggat 900
ggtgccatga ttatcacctc tggtgataga tctgatttga tgttaggttt gatggtttct 960
agattgccag gtgttttgcc aaacatttct gctatcgttt tgaccaatgg taactaccca 1020
cattctaaca cccaagaaat tttgaagggt gttgaagctt tggataagac cggtttgtct 1080
ttgccaattt tctctactcc aaacgatact ttctctaccg ctgatggttt tgctaaggtt 1140
tctactgata ttttgccaac ctccaagttg aagatcgaca gatctaagca attatttgat 1200
gaattcgttg aaaaagaaat gttgatcggt gaattggacc aaggtatggt tgttaataga 1260
tccccaaagc aatttcaaca cttcttgttc tctaagtcca gagccgttca aagacatatt 1320
gtcttgtctg aaggtgaaga tatcagagtt ttacaagccg ccgatcaaat cttgagacaa 1380
aacttgtcta agatcaccat cttgggtaac ccagacgaaa ttttgttgaa cgctaagact 1440
gctaacttgg atttgtctag agctaacatc gttagaccat ccgactctga attattagat 1500
aagtacgttg actacttcta cgaaaaaaga aagcacaagg gtgtcaccaa agaattggct 1560
agagattact gtaaggacga aacttacttc ggtactttga tggtagaatt gggtgatgct 1620
gatggtatgg tttctggtgc ttgtcataca actgctaaca ctattagacc agccttgcaa 1680
ttgattaaga ccgctccaaa tagaccaatc gtcagttctg ttttcttcat gtgtttggaa 1740
gatggtgtca gaatctatgg tgattgtgct gttaatactg atccatctgc acaagatttg 1800
gctcaaattg ctgttactag tgctgaatct gctgaagctt ttggtttgat tccaaaggtt 1860
gctttgttgt cttatgccac tggtgattct aattccggtc caattattga caaggttaga 1920
gaagctacca agatcgctca agaattgaga ccagatttgg atatctacgg tccaatccaa 1980
tatgatgctg cagttgatgc ttctattgcc aagacaaaat tgaaggctat tccatccggt 2040
gctaaagttg gtggtcaagc taatgttttg atcttcccag atttgaacac tggtaacaac 2100
acttacaagg ctgttcaaca atctaccggt tgtattgcta tgggtccaat gttgcaaggt 2160
ttaagaaagc cagttaacga cttgtcaaga ggtgctactg ttaaggatat cgttactact 2220
gttgctatta ccgctattca agccgatcaa gttatcttga aaagagaagc tgaagcagct 2280
actgctaagt tgtaa 2295
<210> 61
<211> 760
<212> PRT
<213> Pythium ultimum
<400> 61
Met Trp Lys Cys Gly Arg Ser Met Arg Arg Met Thr Ala Leu Val Asn
1 5 10 15
Gln Arg Thr Ala Pro His Cys Arg Ala Leu Thr Ala Ala Ala Leu Glu
20 25 30
Gln Gly Arg Val Pro Val Glu Ser Leu Tyr Val Thr Ser Thr Glu Tyr
35 40 45
Thr Lys Lys Thr Ser Pro Val Leu Leu Gly Leu Ala Asn Thr Leu Glu
50 55 60
Gln Lys Phe Ser Ser Val Gly Tyr Phe Arg Pro Ile Ala Pro Gly Lys
65 70 75 80
Ser Ser Gly Ile Glu Asp His His Met Glu Leu Met Lys Ser Glu Leu
85 90 95
Glu Leu Pro Glu Gln Ile Asn Glu Leu Tyr Gly Val Thr Ser Glu Arg
100 105 110
Ala Leu Glu Cys Trp Met Thr Gly Lys Ser Asp Asp Leu Val Glu Glu
115 120 125
Ile Leu Ser Ser Phe Glu His Cys Lys Lys Asn His Asp Phe Met Ile
130 135 140
Ile Glu Gly Ser Pro Val Thr Glu His Glu Ser Ala Met Ser Trp Lys
145 150 155 160
Ile Asn Ile Asp Ile Ala Arg Ala Ile Gly Ser Pro Val Leu Leu Leu
165 170 175
Thr Asp Met Ser Ala Met Ser Tyr Thr Asn Gly Asp Leu Val Asp Glu
180 185 190
Ile Val Ser Arg Thr Val Leu Gly Lys Glu Gln Val Glu Ala Ala Gly
195 200 205
Leu Asn Tyr Phe Gly Thr Ile Ala Asn Arg Val Arg Ser Ala Asp Pro
210 215 220
Thr Thr Met Gln Gly Lys Leu Lys Asp Ala Met Ala Lys Lys Asn Leu
225 230 235 240
Pro Phe Leu Gly Phe Leu Pro His Asp Asn Leu Ile Ala Ser Lys Arg
245 250 255
Leu Asn Glu Val Ala His Lys Leu Gly Ala Lys Gln Leu Phe Gly Ser
260 265 270
Lys Glu Ile Ser Asn Asn Val Ile Val Ser Asp Ala Val Val Ala Thr
275 280 285
Ser His Leu Arg Asp Leu Phe Ala His Leu Lys Lys Tyr Asn Asp Gly
290 295 300
Ile Leu Val Ile Thr Ser Ala Asp Arg Ser Asp Ile Leu Leu Gly Leu
305 310 315 320
Leu Ala Ser Arg Ile Pro Gly Val Leu Pro Asn Val Ala Gly Ile Val
325 330 335
Leu Thr Asn Gly Asp Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Gln
340 345 350
Gly Val Ser Glu Leu Asp Lys Thr Gly Leu Ser Ile Pro Ile Phe Val
355 360 365
Val Pro His Asp Thr Tyr Ser Thr Ala Thr Ala Val Ser His Val Ser
370 375 380
Thr Asp Ile Met Pro Thr Ser Val Arg Lys Ile Gly Gln Cys Lys Asn
385 390 395 400
Leu Phe Asp Gln Phe Ile Glu Lys Thr Asn Leu Ile Gly Glu Leu Asp
405 410 415
Glu Gly Val Val Leu His Arg Ser Pro Lys His Phe Asn His Phe Val
420 425 430
Leu Asn Lys Ala Arg Ala Ala Gln Arg His Ile Val Leu Thr Glu Gly
435 440 445
Glu Asp Ile Arg Ile Leu Gln Ala Ala Asp Glu Ile Leu Arg Gln Arg
450 455 460
Leu Ala Lys Leu Thr Ile Leu Gly Asp Pro Asp Glu Ile Arg Leu His
465 470 475 480
Ala Lys Thr Met Asn Leu Asp Leu Ser Gly Ala Asn Ile Ile Lys Pro
485 490 495
Met Asn Ser Asp Arg Leu Gly Ser Tyr Thr Glu Arg Leu Tyr Glu Met
500 505 510
Arg Lys His Lys Gly Met Thr Lys Glu Ile Ala Arg Asp Thr Ile Ala
515 520 525
Glu Glu Thr Tyr Tyr Gly Thr Met Met Val Glu Met Gly Asp Ala Asp
530 535 540
Gly Met Val Ser Gly Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro
545 550 555 560
Ala Leu Gln Leu Ile Lys Thr Ser Pro Glu Arg Pro Leu Val Ser Ser
565 570 575
Val Phe Phe Met Cys Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys
580 585 590
Ala Val Asn Thr Ser Pro Thr Ala Glu Glu Leu Ala Gln Ile Ala Val
595 600 605
Thr Ser Ala Glu Ser Ala Glu Ala Phe Gly Met Ile Pro Arg Val Ala
610 615 620
Leu Leu Ser Tyr Ala Thr Gly Asp Ser Asn Lys Gly Pro Ile Ile Asp
625 630 635 640
Lys Val Arg Glu Ala Thr Lys Ile Ala Gln Glu Met Arg Pro Asp Leu
645 650 655
Asp Ile Tyr Gly Pro Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile
660 665 670
Ala Lys Gln Lys Leu Lys Ala Asp Ser Thr Gly Ala Arg Val Ala Gly
675 680 685
Arg Ala Asn Val Leu Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr
690 695 700
Tyr Lys Ala Val Gln Gln Ser Thr Gln Cys Val Ala Met Gly Pro Met
705 710 715 720
Leu Gln Gly Leu Arg Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr
725 730 735
Val Lys Asp Ile Ile Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Glu
740 745 750
Gln Met Ile Ala Lys Lys Ala Glu
755 760
<210> 62
<211> 2283
<212> DNA
<213> Pythium ultimum
<400> 62
atgtggaagt gtggtagatc catgagaaga atgactgcct tggttaatca aagaactgct 60
ccacattgca gagctttgac tgctgctgct ttggaacaag gtagagttcc agttgaatcc 120
ttgtacgtta cttctactga atacaccaaa aagacctccc cagttttgtt gggtttggct 180
aatactttag aacaaaagtt ctcctccgtc ggttacttta gaccaattgc tccaggtaaa 240
tcctccggta ttgaagatca tcatatggaa ttgatgaagt ccgaattgga attgccagaa 300
caaatcaacg aattatacgg tgtcacttcc gaaagagctt tggaatgttg gatgactggt 360
aagtctgatg atttggtcga agaaatcttg tcctctttcg aacactgcaa aaagaaccac 420
gatttcatga tcattgaagg ttctccagtt accgaacatg aatctgctat gtcttggaag 480
atcaacattg atattgctag agccattggt tcaccagtct tgttgttgac tgatatgtca 540
gctatgtcct acactaacgg tgatttggtt gacgaaattg tctctagaac cgtcttgggt 600
aaagaacaag ttgaagctgc tggtttgaat tacttcggta ctattgctaa cagagttaga 660
tctgctgatc caactactat gcaaggtaaa ttgaaagatg ctatggccaa gaagaacttg 720
ccatttttgg gttttttgcc acacgataac ttgatcgctt ctaagagatt gaatgaagtt 780
gcccataagt tgggtgccaa gcaattattt ggttccaaag aaatctccaa caacgtcatc 840
gtttctgatg ctgttgttgc tacttctcac ttgagagatt tgttcgccca tttgaaaaag 900
tacaacgatg gtatcttggt tatcacctct gctgatagat ccgatatctt gttaggtttg 960
ttggcctcta gaattccagg tgttttgcca aatgttgctg gtatcgtttt gaccaatggt 1020
gattacccac attctaacac ccaagaaata ttgcaaggtg tctctgaatt ggataagacc 1080
ggtttgtcta ttccaatctt cgttgttcca catgatactt actctactgc tacagctgtt 1140
tctcatgttt ccactgatat tatgccaacc tccgttagaa aaatcggtca atgtaagaac 1200
ttgttcgatc aattcattga aaagaccaac ttgattggtg aattggacga aggtgttgtc 1260
ttgcatagat ctccaaaaca tttcaaccac ttcgttttga acaaagctag agctgctcaa 1320
agacatattg tcttgactga aggtgaagat atcagaatct tgcaagctgc agacgaaatc 1380
ttgagacaaa gattggctaa gttgaccatt ttgggtgatc ctgacgaaat cagattgcat 1440
gctaagacta tgaacttgga tttgtctggt gccaacatta tcaagccaat gaactctgat 1500
agattgggtt cttacaccga aagattatac gaaatgagaa agcacaaggg tatgacaaaa 1560
gaaatcgcca gagataccat tgctgaagaa acttattacg gtactatgat ggtcgaaatg 1620
ggtgatgctg atggtatggt ttctggtgct tgtcatacaa ctgctaacac tattagacca 1680
gccttgcaat tgattaagac ctctccagaa agacctttgg tcagttctgt tttcttcatg 1740
tgtttggaag atggtgtcag aatctatggt gattgtgctg ttaatacttc tccaaccgct 1800
gaagaattgg ctcaaattgc tgttacatct gcagaatctg ctgaagcttt tggtatgatt 1860
ccaagagttg ctttgttgtc ttatgctacc ggtgattcta acaagggtcc aatcattgat 1920
aaggttagag aagctaccaa gatcgctcaa gaaatgagac cagatttgga tatctacggt 1980
ccaatccaat atgatgctgc agttgatgaa tccatagcca agcaaaaatt gaaggctgat 2040
tctactggtg ctagagttgc aggtagagct aatgttttga ttttcccaga tttgaacacc 2100
ggtaacaaca cttacaaagc cgttcaacaa tctacccaat gtgttgctat gggtccaatg 2160
ttgcaaggtt tgagaaaacc agttaacgac ttgtctagag gtgctactgt taaggatatt 2220
atcaccactg ttgctattac cgctattcaa gccgaacaaa tgattgctaa aaaggctgaa 2280
tga 2283
<210> 63
<211> 743
<212> PRT
<213> Saprolegnia diclina
<400> 63
Met Phe Arg Leu Arg Arg Gln Leu Pro Ser Val Ala Arg Arg Trp Tyr
1 5 10 15
Ser Glu Thr His Lys Val Asn Asp Arg Val Ile Val Leu Ser Asn Asp
20 25 30
Ala Thr Ser His Gln Thr Pro Val Leu Leu Gly Leu Met Asn Thr Leu
35 40 45
Ala Ile Lys Tyr His Ser Val Gly Tyr Phe Arg Pro Ile Ala Pro Pro
50 55 60
Leu Gly Ser Asp His His Val Glu Leu Phe Lys Ser Glu Leu Lys Leu
65 70 75 80
Pro Glu Thr Tyr Glu Gln Leu Val Gly Leu His His Asp Asp Val Val
85 90 95
Asn Ala Arg Leu Thr Gly Asp Leu Asp Ile Ile Thr Asp Thr Ile Val
100 105 110
Ala Lys Phe Glu Ala Leu Arg Ala Lys His Asp Phe Val Val Ile Glu
115 120 125
Gly Ala Thr Phe Glu Ser Ala Pro Glu Leu Ala Trp Asp Ile Asn Val
130 135 140
Glu Leu Ala Lys Thr Leu Gly Ala Pro Val Leu Leu Thr Asn Asp Phe
145 150 155 160
Cys Asp Leu Pro Asp Thr Gln Arg Ile Glu Asp Ala Ile Ala Thr Arg
165 170 175
Val Leu Leu Gly Lys Asp Ala Val Asp Ala Ala Gly Leu Thr Tyr Ile
180 185 190
Gly Ser Ile Ala Asn Arg Val Arg Ser Ser Thr Pro Leu Glu Thr Arg
195 200 205
Lys Arg Val Gln Asp Leu Leu Arg Glu Lys Gly Ala Ala Asp Pro Thr
210 215 220
Ile Phe Leu Gly Ala Leu Pro Leu Asp Ser Ile Leu Ala Ser Lys Arg
225 230 235 240
Leu Asn Glu Val Val Ala Gln Leu Gln Ala Thr Gln Leu Tyr Gly Pro
245 250 255
Ala Ser Pro Asn Ser Val Val Val Thr Asp Gly Leu Ile Gly Thr Ser
260 265 270
Asp Leu Lys Glu Leu Phe Gly His Leu Lys Ala His Asp Asp Gly Leu
275 280 285
Leu Val Ile Thr Ser Ala Asp Arg Thr Asp Val Val Leu Gly Leu Leu
290 295 300
Ala Ser Arg Ala Ser Gly Ala Leu Pro Asn Val Ala Gly Val Ile Leu
305 310 315 320
Thr Asn Gly Ser Tyr Pro Gln Asp His Val Lys Asp Ile Leu Asp Gly
325 330 335
Met Ala Lys Ile Asp Asn Ala Thr Ile Pro Ile Tyr Thr Val Glu Gly
340 345 350
Asp Ala Tyr Lys Thr Ala Asn Ala Leu Ser Arg Val Thr Cys Asp Ile
355 360 365
Leu Pro Thr Ser Gln Thr Lys Ile Gln Gln Ser Asn Ile Leu Phe Asp
370 375 380
Lys Phe Val Ser Arg Ser Ala Leu Met Asp Thr Val Cys Gln Ala Val
385 390 395 400
Lys Ser Thr Lys Arg Thr Pro Lys Gln Phe Lys His Phe Leu Phe Ser
405 410 415
Lys Ala Arg Lys Val Gln Gln His Ile Val Leu Thr Glu Gly Glu Asp
420 425 430
Asp Arg Ile Leu Gln Ala Ala Asp Glu Val Leu Arg Arg Asp Ile Ala
435 440 445
Lys Ile Thr Ile Leu Gly Asp Val Asp Ser Ile Ala Ala Arg Ala Lys
450 455 460
Thr Leu Arg Leu Asp Leu Ser Ala Ala Ser Ile Ile Asp Pro Ser Lys
465 470 475 480
Ala Ala Asp Leu Asp Leu Leu Ala Ala Arg Phe Tyr Glu Lys Arg Lys
485 490 495
Val Lys Gly Val Ser Leu Glu Phe Ala Arg Glu Ser Ala Ser Glu Ala
500 505 510
Thr Cys Tyr Gly Thr Leu Met Val Glu Met Gly Leu Ala Asp Gly Met
515 520 525
Val Ser Gly Ala Cys His Thr Thr Ala Asn Thr Val Arg Pro Ala Leu
530 535 540
Gln Leu Ile Lys Thr Arg Pro Asp Arg Pro Leu Val Ser Ser Ile Phe
545 550 555 560
Phe Met Cys Leu Glu Asp Asp Val Val Val Tyr Gly Asp Cys Ala Ile
565 570 575
Asn Thr Asp Pro Thr Ala Glu Asp Leu Ala Asn Ile Ala Val Gln Ser
580 585 590
Ala Glu Ser Ala Arg Ala Phe Gly Met Glu Pro Arg Val Ala Leu Leu
595 600 605
Ser Tyr Ala Thr Gly Asp Ser Asn Lys Gly Pro Ile Ile Asp Lys Val
610 615 620
Arg Glu Ala Thr Lys Leu Ala Gln Lys Met Ala Pro Glu Ile Pro Met
625 630 635 640
Tyr Gly Pro Ile Gln Tyr Asp Ala Ala Met Asn Pro Leu Ile Ala Lys
645 650 655
Gln Lys Val Lys Gly Leu Lys Lys Thr Glu Met Glu Val Ala Gly Asn
660 665 670
Ala Asn Val Leu Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr
675 680 685
Lys Ala Val Gln Gln Ser Thr Asn Cys Leu Ala Met Gly Pro Met Leu
690 695 700
Gln Gly Leu Asn Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val
705 710 715 720
Gly Asp Ile Val Thr Thr Ile Ala Ile Thr Ala Ile Gln Ala His Gln
725 730 735
Met Asn Glu Ala Ala Ser Thr
740
<210> 64
<211> 2232
<212> DNA
<213> Saprolegnia diclina
<400> 64
atgttcagat tgagaagaca attgccatcc gttgctagaa gatggtattc tgaaactcac 60
aaggttaacg atagagtcat cgttttgtct aacgatgcta cttctcatca aaccccagtt 120
ttgttgggtt taatgaacac cttggccatc aagtatcatt ccgttggtta ctttagacca 180
attgctccac cattgggttc tgatcatcat gtcgaattat tcaagtccga attgaagttg 240
ccagaaacct acgaacaatt ggttggttta catcacgatg atgttgttaa cgctagattg 300
actggtgatt tggatattat caccgatacc atcgttgcta agttcgaagc tttgagagct 360
aagcacgatt tcgttgttat tgaaggtgct acttttgaat ccgctccaga attggcttgg 420
gatattaacg ttgaattggc taaaactttg ggtgctcctg ttttgttgac taacgatttt 480
tgtgatttgc cagacaccca aagaatcgaa gatgctattg ctaccagagt cttgttgggt 540
aaagatgctg ttgatgctgc tggtttgact tacattggtt ccattgctaa tagagtcaga 600
tcttctactc cattggaaac cagaaagaga gttcaagact tgttgagaga aaaaggtgct 660
gctgatccaa ctattttctt gggtgctttg ccattggatt ctattttggc ttctaagaga 720
ttgaacgaag ttgtcgctca attgcaagct actcaattat atggtccagc ttctccaaac 780
tctgttgttg ttactgatgg tttgatcggt acttccgatt tgaaagaatt attcggtcat 840
ttgaaggccc atgatgacgg tttgttggtt attacttctg ctgatagaac cgatgttgtc 900
ttgggtttgt tagcttctag agcttctggt gctttaccaa atgttgctgg tgttattttg 960
accaacggtt cttatccaca agatcacgtt aaggatatct tggatggtat ggccaagatt 1020
gataacgcta ctattccaat ctacaccgtt gaaggtgatg cttacaaaac tgctaatgcc 1080
ttgtctagag ttacctgcga tattttgcca acctcccaaa ctaagatcca acaatccaat 1140
atcttgttcg acaagttcgt ttccagatcc gctttgatgg atactgtttg tcaagctgtt 1200
aagtctacta agagaacccc aaaacaattc aagcacttct tgttctctaa ggccagaaag 1260
gttcaacaac atattgtctt gactgaaggt gaagatgaca gaatcttgca agctgctgat 1320
gaagttttga gaagagatat tgctaagatc accatcttgg gtgatgttga ttctattgct 1380
gctagagcta agaccttgag attggatttg tctgctgcct ctattatcga tccatctaaa 1440
gctgcagatt tggacttgtt agctgccaga ttttacgaaa aaagaaaggt caagggtgtc 1500
tccttggaat ttgctagaga atctgcttct gaagctactt gttatggtac tttgatggtc 1560
gaaatgggtt tggctgacgg tatggtttca ggtgcttgtc atacaacagc taatactgtt 1620
agaccagcct tacaattgat taagaccaga ccagatagac cattggtttc ttccattttc 1680
ttcatgtgct tggaagatga tgtcgttgtt tatggtgatt gcgccattaa cacagatcca 1740
actgctgaag atttggctaa cattgctgtt caatctgctg aatcagctag agcatttggt 1800
atggaaccta gagttgcttt gttgtcttat gctacaggtg attctaacaa gggtccaatc 1860
attgataagg ttagagaagc tactaagttg gctcaaaaaa tggctccaga aattccaatg 1920
tacggtccaa ttcaatacga tgctgctatg aatccattga tcgctaagca aaaagtcaag 1980
ggtttgaaaa agaccgaaat ggaagttgct ggtaacgcca atgttttgat tttcccagat 2040
ttgaacactg gtaacaacac ctacaaagcc gttcaacaat ctacaaactg tttggctatg 2100
ggtccaatgt tgcaaggttt gaacaaacca gttaacgact tatctagagg tgctacagtt 2160
ggtgatatcg ttactactat tgctattacc gctatccaag cccatcaaat gaatgaagct 2220
gcttctactt ga 2232
<210> 65
<211> 763
<212> PRT
<213> Saprolegnia parasitica
<400> 65
Met Leu Arg Ala Ser Arg His Leu Leu Pro Thr Arg Arg Ser Ala Leu
1 5 10 15
Leu Ala Arg Ala Ala Thr Ser Arg Leu Phe Ser Asp Ala Pro His Ala
20 25 30
Asn Asp Arg Val Phe Val Met Ser Asn Asp Thr Thr Ser His Gln Ser
35 40 45
Pro Val Leu Leu Gly Leu Met Asn Thr Leu Ser Val Lys Tyr Pro Ser
50 55 60
Val Gly Tyr Phe Arg Pro Ile Ala Pro Asn Lys Asp Pro Arg Val Ser
65 70 75 80
Asp His His Val Glu Val Met Lys Ser Glu Phe Lys Leu Pro Glu Glu
85 90 95
Lys Asp Gln Leu Val Gly Leu Tyr Asn Asp Asp Val Val Gln Ala Arg
100 105 110
Leu Lys Gly Asp Leu Asp Ser Val Thr Asp Thr Ile Ile Ser Lys Phe
115 120 125
Glu Ala Leu Arg Ala Lys His Asp Phe Val Val Ile Glu Gly Ala Met
130 135 140
Phe Glu Ser Ala Pro Asp Leu Ala Trp Asp Ile Asn Val Glu Leu Ala
145 150 155 160
Lys Thr Leu Gly Ala Pro Val Leu Leu Thr Asn Asp Leu Ala Asp Val
165 170 175
Glu Ala Asp Glu Ser Leu Ser Glu Asp Glu Arg Thr Lys Lys Leu Val
180 185 190
Asp Thr Ile Val Thr Arg Val Leu Leu Ala Lys Asp Ala Val Gln Asp
195 200 205
Ala Gly Leu Thr Tyr Val Gly Ser Ile Ala Asn Arg Val His Thr Lys
210 215 220
Asp Pro Ile Glu Ile Arg Lys Arg Val Gln Ala Ile Leu Ala Glu Lys
225 230 235 240
Gly Glu Thr Asn Ser Ile Phe His Gly Ala Leu Pro Leu Asp Ser Ile
245 250 255
Leu Ala Ser Lys Arg Leu Asn Glu Val Val Asp Gln Leu His Ala Thr
260 265 270
Gln Leu Tyr Gly Pro Pro Ser Pro Asn Ser Val Val Val Thr Asp Gly
275 280 285
Phe Val Gly Thr Ser Asp Leu Lys Asp Leu Phe Gly His Met Lys Lys
290 295 300
His Asp Asp Gly Leu Leu Val Ile Val Ser Ala Asp Arg Thr Asp Val
305 310 315 320
Met Leu Gly Leu Leu Val Ser Lys Leu Ser Gly Ala Leu Pro Asn Val
325 330 335
Ala Ala Val Ile Leu Thr Asn Gly Lys Phe Pro Gln Asp His Val Lys
340 345 350
Glu Ile Leu Glu Gly Met Ala Lys Ile Asp Asn Ala Thr Ile Pro Ile
355 360 365
Tyr Thr Val Glu Gly Asp Ser Tyr Lys Thr Ala Asn Ala Leu Ser Arg
370 375 380
Val Thr Cys Asp Ile Leu Pro Thr Ser Gln Thr Lys Ile Gln Gln Ser
385 390 395 400
Tyr Ile Leu Phe Asp Lys Tyr Val Ala Arg Asp Ser Val Val Gly Gly
405 410 415
Val Ser Lys Glu Met Ser Thr Lys Arg Thr Pro Lys Gln Phe Lys His
420 425 430
Phe Leu Phe Ser Lys Ala Arg Lys Val Gln Gln His Ile Val Leu Thr
435 440 445
Glu Gly Glu Asp Asp Arg Ile Leu Gln Ala Ala Asp Glu Val Leu Arg
450 455 460
Arg Asp Ile Ala Lys Leu Thr Ile Leu Gly Asp Val Glu Ser Ile Ala
465 470 475 480
Ala Arg Ala Lys Thr Leu Arg Leu Asp Leu Ser Ala Ala Ser Ile Val
485 490 495
Asp Pro Ser Lys Ser Ala Asp Leu Asp Met Tyr Ala Asp Arg Phe Tyr
500 505 510
Glu Lys Arg Lys Ile Lys Gly Val Ser Arg Glu Val Ala Arg Glu Ser
515 520 525
Ala Ala Glu Ala Thr Cys Tyr Gly Thr Leu Met Val Glu Met Gly Leu
530 535 540
Ala Asp Gly Met Val Ser Gly Ala Cys His Thr Thr Ala Asn Thr Val
545 550 555 560
Arg Pro Ala Leu Gln Leu Ile Lys Thr Arg Pro Asp Arg Pro Leu Val
565 570 575
Ser Ser Val Phe Phe Met Cys Leu Asp His Asp Val Val Leu Tyr Gly
580 585 590
Asp Cys Ala Ile Asn Thr Asp Pro Thr Ala Glu Asp Leu Ala Asn Ile
595 600 605
Ala Val Gln Ser Ala Glu Ser Ala Ile Ala Phe Gly Met Glu Pro Arg
610 615 620
Val Ala Leu Leu Ser Tyr Ala Thr Gly Asp Ser Asn Lys Gly Pro Ile
625 630 635 640
Ile Asp Lys Val Arg Glu Ala Thr Lys Leu Ala Gln Lys Met Ala Pro
645 650 655
Glu Ile Pro Met Tyr Gly Pro Ile Gln Tyr Asp Ala Ala Met Asn Pro
660 665 670
Leu Ile Ala Lys Gln Lys Val Lys Gly Leu Lys Lys Thr Glu Met Glu
675 680 685
Val Ala Gly Asn Ala Asn Val Leu Ile Phe Pro Asp Leu Asn Thr Gly
690 695 700
Asn Asn Thr Tyr Lys Ala Val Gln Gln Ser Thr Asn Cys Leu Ala Met
705 710 715 720
Gly Pro Met Leu Gln Gly Leu Asn Lys Pro Val Asn Asp Leu Ser Arg
725 730 735
Gly Ala Thr Val Gly Asp Ile Val Thr Thr Ile Ala Ile Thr Ala Ile
740 745 750
Gln Ala Asp Gln Met Asn Glu Ala Ala Lys Pro
755 760
<210> 66
<211> 2292
<212> DNA
<213> Saprolegnia parasitica
<400> 66
atgctgcgag cctcccgaca cctgctgccc acccgacgat ccgccctgct ggcccgagcc 60
gccacctccc gactgttctc cgacgccccc cacgccaacg accgagtctt cgtcatgtcc 120
aacgacacca cctcccacca gtcccccgtc ctgctgggcc tgatgaacac cctgtccgtc 180
aagtacccct ccgtcggcta cttccgaccc atcgccccca acaaggaccc ccgagtctcc 240
gaccaccacg tcgaggtcat gaagtccgag ttcaagctgc ccgaggagaa ggaccagctg 300
gtcggcctgt acaacgacga cgtcgtccag gcccgactga agggcgacct ggactccgtc 360
accgacacca tcatctccaa gttcgaggcc ctgcgagcca agcacgactt cgtcgtcatc 420
gagggcgcca tgttcgagtc cgcccccgac ctggcctggg acatcaacgt cgagctggcc 480
aagaccctgg gcgcccccgt cctgctgacc aacgacctgg ccgacgtcga ggccgacgag 540
tccctgtccg aggacgagcg aaccaagaag ctggtcgaca ccatcgtcac ccgagtcctg 600
ctggccaagg acgccgtcca ggacgccggc ctgacctacg tcggctccat cgccaaccga 660
gtccacacca aggaccccat cgagatccga aagcgagtcc aggccatcct ggccgagaag 720
ggcgagacca actccatctt ccacggcgcc ctgcccctgg actccatcct ggcctccaag 780
cgactgaacg aggtcgtcga ccagctgcac gccacccagc tgtacggccc cccctccccc 840
aactccgtcg tcgtcaccga cggcttcgtc ggcacctccg acctgaagga cctgttcggc 900
cacatgaaga agcacgacga cggcctgctg gtcatcgtct ccgccgaccg aaccgacgtc 960
atgctgggcc tgctggtctc caagctgtcc ggcgccctgc ccaacgtcgc cgccgtcatc 1020
ctgaccaacg gcaagttccc ccaggaccac gtcaaggaga tcctggaggg catggccaag 1080
atcgacaacg ccaccatccc catctacacc gtcgagggcg actcctacaa gaccgccaac 1140
gccctgtccc gagtcacctg cgacatcctg cccacctccc agaccaagat ccagcagtcc 1200
tacatcctgt tcgacaagta cgtcgcccga gactccgtcg tcggcggcgt ctccaaggag 1260
atgtccacca agcgaacccc caagcagttc aagcacttcc tgttctccaa ggcccgaaag 1320
gtccagcagc acatcgtcct gaccgagggc gaggacgacc gaatcctgca ggccgccgac 1380
gaggtcctgc gacgagacat cgccaagctg accatcctgg gcgacgtcga gtccatcgcc 1440
gcccgagcca agaccctgcg actggacctg tccgccgcct ccatcgtcga cccctccaag 1500
tccgccgacc tggacatgta cgccgaccga ttctacgaga agcgaaagat caagggcgtc 1560
tcccgagagg tcgcccgaga gtccgccgcc gaggccacct gctacggcac cctgatggtc 1620
gagatgggcc tggccgacgg catggtctcc ggcgcctgcc acaccaccgc caacaccgtc 1680
cgacccgccc tgcagctgat caagacccga cccgaccgac ccctggtctc ctccgtcttc 1740
ttcatgtgcc tggaccacga cgtcgtcctg tacggcgact gcgccatcaa caccgacccc 1800
accgccgagg acctggccaa catcgccgtc cagtccgccg agtccgccat cgccttcggc 1860
atggagcccc gagtcgccct gctgtcctac gccaccggcg actccaacaa gggccccatc 1920
atcgacaagg tccgagaggc caccaagctg gcccagaaga tggcccccga gatccccatg 1980
tacggcccca tccagtacga cgccgccatg aaccccctga tcgccaagca gaaggtcaag 2040
ggcctgaaga agaccgagat ggaggtcgcc ggcaacgcca acgtcctgat cttccccgac 2100
ctgaacaccg gcaacaacac ctacaaggcc gtccagcagt ccaccaactg cctggccatg 2160
ggccccatgc tgcagggcct gaacaagccc gtcaacgacc tgtcccgagg cgccaccgtc 2220
ggcgacatcg tcaccaccat cgccatcacc gccatccagg ccgaccagat gaacgaggcc 2280
gccaagccct aa 2292
<210> 67
<211> 422
<212> PRT
<213> Selaginella moellendorffii
<400> 67
Met Trp Gly Ile Asp Arg Arg Lys Ala Leu Glu Phe Leu Ala Asn Gly
1 5 10 15
Asp Leu Asp Glu Met Leu Glu Gln Ile Tyr Val Ala Tyr Glu Glu Leu
20 25 30
Lys Arg Arg His Glu Cys Val Leu Ile Glu Gly Thr Gln Phe Cys Arg
35 40 45
Asp Ala Ser Ala Leu Asp Ala His Ile Ala Ser Ala Leu Gly Ser Pro
50 55 60
Val Leu Leu Ala Thr Asn Val Asp Ala Leu Arg Gln Leu Trp Ser Ser
65 70 75 80
Lys Asp His Ala Gly Asn Leu Gln Asp Trp Ala Thr Glu Ile Ala Thr
85 90 95
Tyr Thr Arg Cys Ser Ala Leu Ala Phe Glu Lys Leu Lys Val Arg Val
100 105 110
Val Gly Gly Phe Val Tyr Gly Ser Ser Asp Ala Pro Asp Met Arg Lys
115 120 125
Val Phe Ser Lys Trp Lys Leu Gln Phe Val Gly Ala Leu Pro Gly Phe
130 135 140
Asp Glu Arg Glu Asn Pro Val Lys Ala Phe Ala Asn Asn Ile Glu Met
145 150 155 160
Glu Ala Leu Lys Arg Asn Met Pro Glu Glu Asn Ala Ala Arg Val Ser
165 170 175
Pro Leu Leu Phe Arg Asn Ser Leu Phe Ser Arg Ala Lys Glu Asn Asn
180 185 190
Gln Val Ile Leu Leu Pro Glu Gly Asp Glu Pro Arg Thr Val Gln Ala
195 200 205
Ala Gly Phe Ile Leu Gln His Gly Leu Cys Ser Leu Ile Leu Leu Gly
210 215 220
Glu Arg Glu Lys Leu Leu Glu Ala Ala Lys Val Ser Asn Val Asp Leu
225 230 235 240
Arg Ser Ala Ile Ile Lys Asp Pro Ser Asp Pro Gln Glu Leu Glu Lys
245 250 255
Tyr Ala Thr Val Tyr Tyr Gln Thr Arg Lys His Lys Gly Met Thr Leu
260 265 270
Glu Lys Ala Arg Glu Ile Leu Gly Asn Asp Pro Ile Thr Leu Gly Thr
275 280 285
Cys Met Val Ser Ala Gly Asp Ala Asp Gly Met Val Cys Gly Ala Val
290 295 300
His Thr Thr Ala Asn Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr
305 310 315 320
Asp Pro Ala Thr Pro Ile Val Ser Ser Val Met Phe Ile Cys Leu Glu
325 330 335
Asp Ala Val Val Ala Tyr Ala Asp Val Ala Ile Asn Ala Ser Pro Ser
340 345 350
Ala Asp Glu Leu Ala Thr Ile Ala Ile Ala Ser Ala Asp Thr Val Thr
355 360 365
Ala Phe Gly Leu Glu Pro Arg Val Ala Leu Leu Ser Tyr Ala Thr Gly
370 375 380
Asp Ser Asn Ala Gly Arg Gly Arg Cys Gln His Arg Lys Ile Gln Thr
385 390 395 400
Pro Gly Phe Ile Thr Arg Gly Thr Ile Pro Gly Ala Ala Ser Ile Cys
405 410 415
Leu Pro Arg Ser Gln Gly
420
<210> 68
<211> 1269
<212> DNA
<213> Selaginella moellendorffii
<400> 68
atgtggggta tcgatagaag aaaggctttg gaatttttgg ccaatggtga tttggacgaa 60
atgttggaac aaatctacgt tgcctacgaa gaattgaaga gaagacatga atgcgtcttg 120
atcgaaggta ctcaattctg tagagatgct tctgctttgg atgctcatat tgcttcagct 180
ttaggttctc cagttttgtt ggctactaat gttgatgcct tgagacaatt gtggtcctct 240
aaagatcatg ctggtaactt gcaagattgg gctactgaaa ttgctactta cactagatgt 300
tccgctttgg cttttgaaaa gttgaaggtt agagttgttg gtggtttcgt ttacggttct 360
tctgatgctc cagatatgag aaaggttttc tctaagtgga agttgcaatt cgttggtgct 420
ttgccaggtt ttgacgaaag agaaaatcca gttaaggctt tcgccaacaa cattgaaatg 480
gaagccttga aaagaaacat gccagaagaa aacgctgcta gagtttcacc tttgttgttc 540
agaaactcct tgttctccag agccaaagaa aacaatcaag tcatcttgtt gcctgaaggt 600
gatgaaccta gaactgttca agctgctggt tttatcttgc aacatggttt gtgttccttg 660
atcttgttgg gtgaaagaga aaagttgttg gaagctgcta aggtttccaa cgttgatttg 720
agatctgcca ttatcaagga tccatctgat ccacaagaat tggaaaagta cgctaccgtt 780
tactaccaaa ctagaaaaca taagggtatg accttggaaa aggccagaga aattttgggt 840
aacgatccaa ttactttggg tacttgtatg gtttctgctg gtgatgctga tggtatggtt 900
tgcggtgctg ttcatacaac tgctaatact gttagaccag ccttgcaaat tatcaagact 960
gatccagcta ctccaatcgt ttcttccgtt atgttcattt gcttggaaga tgctgttgtt 1020
gcttacgctg atgttgctat taacgcttct ccatctgctg atgaattagc tactattgct 1080
attgcttctg ccgatactgt tactgctttt ggtttggaac ctagagttgc tttgttgtct 1140
tatgctactg gtgattctaa tgctggtaga ggtagatgtc aacacagaaa gattcaaacc 1200
ccaggtttca ttaccagagg tactattcca ggtgctgctt ctatttgttt gccaagatct 1260
caaggttaa 1269
<210> 69
<211> 240
<212> PRT
<213> Tetraselmis sp.
<220>
<221> MOD_RES
<222> (98)..(98)
<223> 任意氨基酸
<220>
<221> MOD_RES
<222> (100)..(100)
<223> 任意氨基酸
<220>
<221> MOD_RES
<222> (139)..(139)
<223> 任意氨基酸
<220>
<221> MOD_RES
<222> (183)..(183)
<223> 任意氨基酸
<400> 69
Met Arg Pro Ala Leu Gln Val Ile Lys Cys Ala Pro Gly Ala Asn Met
1 5 10 15
Val Ser Ser Ala Phe Phe Met Leu Leu Asp Ser Gly Val Lys Val Phe
20 25 30
Ala Asp Cys Ala Ile Val Glu Ser Pro Thr Ala Glu Gln Leu Ala Glu
35 40 45
Ile Gly Ala Ala Ser Ala Val Thr Ala Ala Ser Phe Gly Leu Ser Pro
50 55 60
Arg Ile Ala Met Leu Ser Tyr Ala Thr Gly Asp Ser Asn Ser Gly Pro
65 70 75 80
Met Ile Thr Lys Val Arg Glu Ala Thr Glu Leu Leu Arg Arg His Pro
85 90 95
Leu Xaa Val Xaa Arg Gly Tyr Pro Val Glu Gly Pro Ile Gln Phe Asp
100 105 110
Ala Ala Val Asp Pro Met Val Ala Glu Val Lys Phe Arg Gly Ser Pro
115 120 125
Gly Pro Val Ala Gly Arg Ala Asn Val Cys Xaa Phe Pro Asp Leu Asn
130 135 140
Ala Gly Asn Asn Ala Tyr Lys Ala Val Gln Gln Ala Ser Gly Cys Val
145 150 155 160
Ala Ile Gly Pro Val Met Gln Gly Leu Lys Leu Pro Val Asn Asp Leu
165 170 175
Ser Arg Gly Cys Thr Val Xaa Asp Ile Val Gln Thr Val Ile Val Thr
180 185 190
Cys Val Gln Ala Ile Ala Ala Lys Gln Ala Gln Met Pro Glu Glu Glu
195 200 205
Val Leu Ser Pro Lys Pro Ile Lys Asp Arg His Leu Leu Ile Lys Ala
210 215 220
Val Glu Ser Arg Gly Ser Leu Asn Asn Leu Thr Ser His Pro Leu Asn
225 230 235 240
<210> 70
<211> 723
<212> DNA
<213> Tetraselmis sp.
<220>
<221> modified_base
<222> (292)..(294)
<223> a, c, t, g, 未知或其它
<220>
<221> modified_base
<222> (298)..(300)
<223> a, c, t, g, 未知或其它
<220>
<221> modified_base
<222> (415)..(417)
<223> a, c, t, g, 未知或其它
<220>
<221> modified_base
<222> (547)..(549)
<223> a, c, t, g, 未知或其它
<400> 70
atgcgacccg ccctgcaggt catcaagtgc gcccccggcg ccaacatggt ctcctccgcc 60
ttcttcatgc tgctggactc cggcgtcaag gtcttcgccg actgcgccat cgtcgagtcc 120
cccaccgccg agcagctggc cgagatcggc gccgcctccg ccgtcaccgc cgcctccttc 180
ggcctgtccc cccgaatcgc catgctgtcc tacgccaccg gcgactccaa ctccggcccc 240
atgatcacca aggtccgaga ggccaccgag ctgctgcgac gacaccccct gnnngtcnnn 300
cgaggctacc ccgtcgaggg ccccatccag ttcgacgccg ccgtcgaccc catggtcgcc 360
gaggtcaagt tccgaggctc ccccggcccc gtcgccggcc gagccaacgt ctgcnnnttc 420
cccgacctga acgccggcaa caacgcctac aaggccgtcc agcaggcctc cggctgcgtc 480
gccatcggcc ccgtcatgca gggcctgaag ctgcccgtca acgacctgtc ccgaggctgc 540
accgtcnnng acatcgtcca gaccgtcatc gtcacctgcg tccaggccat cgccgccaag 600
caggcccaga tgcccgagga ggaggtcctg tcccccaagc ccatcaagga ccgacacctg 660
ctgatcaagg ccgtcgagtc ccgaggctcc ctgaacaacc tgacctccca ccccctgaac 720
taa 723
<210> 71
<211> 803
<212> PRT
<213> Volvox carteri
<400> 71
Met Ala Ala His Leu Gln Arg Cys Arg Pro Cys Asp Ser Asp Thr Gly
1 5 10 15
Ala Ser Phe Ile Pro Glu Ser Ala Leu Thr Arg Arg Ala Ala Leu Pro
20 25 30
Pro Ala Gly Arg Ile Leu His Arg Arg Ser Tyr Ser Ala Val Ala Pro
35 40 45
Ile Ala Arg Ala Ser Arg Ser Ser Ser Lys Gln Ser Ser Asn Ser Gly
50 55 60
Leu Gln Ser Leu Phe Leu Ser Asp Ile Ser Leu Ile Gly Gln Arg Thr
65 70 75 80
Pro Leu Leu Leu Gly Phe Phe Asn Tyr Phe Glu Arg His Leu Pro His
85 90 95
Val Gly Phe Phe Glu Pro Ile Ala His Glu Ala Leu Ala Ser Ser Glu
100 105 110
Leu Lys Ile Asp Arg His Val Glu Leu Val Tyr Lys Val Phe Asn Leu
115 120 125
Lys Gly Asp Ala Thr Arg Met Thr Gly Val Gln Asp Ala Glu Ala Ala
130 135 140
Arg Met Ile Ala Asn Gly Gln His Ser Glu Leu Leu Asp Arg Ile Tyr
145 150 155 160
Ala Asn Phe Val Ser Tyr Lys Glu Gly His Asp Leu Val Leu Val Glu
165 170 175
Gly Pro Gly Pro Leu Met Gly Gly Thr Glu Leu Asp Ala Gln Ile Ala
180 185 190
Ala Thr Ile Asn Ala Pro Val Leu Met Thr Met Thr Gly Ser Pro Asn
195 200 205
Cys Ser Val Ser Asp Tyr Tyr Asn Arg Ala Met Val Lys Arg Gln Val
210 215 220
Phe Leu Asp His Lys Val Glu Val Leu Gly Leu Val Met Asn Gly Leu
225 230 235 240
Pro Arg Asn Ser His Ala Leu Met Thr Ala Gln Leu Lys Ala Arg Phe
245 250 255
Asn Gln Ser Gly Leu Pro Phe Ala Gly Ala Ile Pro Gln Asp Pro Ile
260 265 270
Leu Lys Asn Val Arg Leu Asp Glu Val Gln Thr Ala Leu Gln Ala Val
275 280 285
Arg Leu Tyr Gly Asp Ser Leu Leu Thr Asp Val Glu Phe Asp Asp Val
290 295 300
Val Val Gly Cys Gln Arg Leu Glu Glu Leu Leu Glu Ile Leu Gly Glu
305 310 315 320
Arg Pro Gly Gly Arg Pro Leu Val Ile Thr Ser Ala Asp Arg Leu Asp
325 330 335
Ile Val Leu Gly Leu Leu Ala Ala Gln Leu Ser Val Ser Gly Pro Gly
340 345 350
Val Ala Gly Val Leu Leu Thr Gln Ala Gly Ser Ser Arg Ser Gly Arg
355 360 365
Asn Tyr Ala Arg Asp Thr Ile Asp Arg Ile Phe Ala Gly Leu Val Asn
370 375 380
Ser Gly Leu Tyr Lys Gly Ser Leu Leu Pro Arg Leu Phe Glu Gln Tyr
385 390 395 400
Val Asp Ala Asn Ala Val Val Ala Glu Leu Gln Arg Ile Lys Pro Thr
405 410 415
Arg Met Thr Pro Lys Met Phe Met His Thr Leu Lys Thr Met Cys Arg
420 425 430
Glu Asn Pro Gln His Ile Ile Leu Pro Glu Ser Asp Asp Lys Arg Val
435 440 445
Leu Ala Ala Ala Ala Asp Val Thr Thr Arg Gly Leu Ala Lys Ile Thr
450 455 460
Leu Leu Gly Asp Pro Thr Thr Ile Thr Ala Glu Ala Ala Lys Leu Gly
465 470 475 480
Leu Asp Leu Ser Gln Cys Asn Ile His Asn Pro Asn Thr Ala Gly Arg
485 490 495
Phe Asp Ala Tyr Ala Glu Leu Leu Val Glu Leu Arg Lys His Lys Gly
500 505 510
Met Thr Pro Asp Arg Ala Leu Asp Thr Leu His Gly Asp Met Asn Phe
515 520 525
Tyr Ala Thr Met Met Ile Ala Ala Gly Asp Ala Asp Gly Met Val Ser
530 535 540
Gly Ala Cys His Thr Thr Ala Ser Thr Val Arg Pro Ala Met Gln Val
545 550 555 560
Leu Lys Ser Ala Asp Ser Pro Leu Val Ser Ser Val Phe Ile Met Cys
565 570 575
Leu Pro Asp Arg Val Val Val Tyr Gly Asp Cys Ala Val Asn Val Asn
580 585 590
Pro Thr Ala Ala Glu Leu Ala Thr Ile Ala Ile Thr Ser Ala Asp Thr
595 600 605
Ala Ala Ala Phe Gly Ile Glu Pro Arg Val Ala Met Leu Ser Tyr Ser
610 615 620
Thr Leu Gly Ser Gly Thr Gly Pro Asp Val Leu Lys Val Glu Glu Ala
625 630 635 640
Val Ala Leu Ala Lys Ala Arg Arg Pro Asp Leu Lys Ile Glu Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Ile Asp Pro Lys Val Ala Ala Val Lys Val
660 665 670
Thr Gly Gly Ser Glu Val Ala Gly Lys Ala Thr Val Phe Val Phe Pro
675 680 685
Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln Gln Ser Ser
690 695 700
Gly Ala Ile Ala Met Gly Pro Ile Met Gln Val Pro Val Trp Gly Gly
705 710 715 720
Trp Pro Tyr Leu Pro Trp Pro Gly Leu Leu Lys Pro Val Asn Asp Leu
725 730 735
Ser Arg Gly Cys Thr Val Pro Asp Ile Val Asn Thr Ile Cys Val Thr
740 745 750
Ser Ile Gln Ala Ser Arg Leu Arg Arg Gly Asn Arg Gln Ser Ala Asp
755 760 765
Ser Thr Pro Thr Gln Ser Met Asp Gly Gly Pro Gly Pro Ser Asn Gly
770 775 780
Asn Gly Asn Gly Asn Gly Asn Gly Ser Gly Val Ile Pro Pro Gln Leu
785 790 795 800
Ala Ile Val
<210> 72
<211> 2412
<212> DNA
<213> Volvox carteri
<400> 72
atggctgctc acttgcaaag atgtagacca tgtgattctg atactggtgc ttctttcatt 60
ccagaatctg ctttgactag aagagctgct ttgccaccag caggtagaat attgcataga 120
agatcttatt ctgccgttgc tccaattgct agagcttcta gatcttcctc taaacaatct 180
tccaactccg gtttacaatc cttgttcttg tccgatattt ccttgattgg tcaaagaacc 240
cctttgttgt tgggtttctt taactacttc gaaagacact tgccacacgt tggttttttt 300
gaacctattg ctcatgaagc tttggcctct tccgaattga aaatcgatag acatgttgaa 360
ttggtctaca aggttttcaa cttgaagggt gatgctacta gaatgactgg tgttcaagat 420
gctgaagctg ctagaatgat tgctaatggt caacactccg aattattgga tagaatctac 480
gccaacttcg tttcctacaa agaaggtcac gatttggttt tggttgaagg tccaggtcca 540
ttgatgggtg gtactgaatt ggatgctcaa attgctgcta ctattaacgc tccagttttg 600
atgactatga ctggttctcc aaactgctct gtttctgatt actacaatag agccatggtc 660
aagagacaag ttttcttgga tcataaggtt gaagtcttgg gtttggttat gaatggtttg 720
ccaagaaact ctcatgcttt gatgacagct caattgaagg ccagattcaa tcaatcaggt 780
ttgccatttg ctggtgctat tccacaagat ccaattttga agaacgtcag attggatgaa 840
gttcaaactg cattgcaagc cgttagatta tacggtgatt ctttgttgac tgacgttgaa 900
ttcgatgatg ttgttgtcgg ttgtcaaaga ttggaagaat tattagaaat cttgggtgaa 960
agaccaggtg gtagaccatt ggttattaca tctgctgata gattggatat cgttttgggt 1020
ttgttagctg cccaattgtc tgtttcaggt cctggtgttg ctggtgtttt gttaactcaa 1080
gctggttctt caagatccgg tagaaattat gctagagata ccatcgatag aattttcgct 1140
ggtttggtca attccggttt gtacaagggt tctttattgc caagattatt cgaacaatac 1200
gttgatgcta acgctgttgt tgctgaattg caaagaatca agccaacaag aatgacccca 1260
aagatgttca tgcatacctt gaaaactatg tgcagagaaa accctcaaca catcattttg 1320
ccagaatccg atgataagag agttttggct gctgctgcag atgttactac tagaggtttg 1380
gctaagatta ccttgttggg tgatccaact actattactg ctgaagcagc aaagttgggt 1440
ttagatttgt ctcaatgcaa catccataac ccaaatactg ctggtagatt cgatgcttac 1500
gctgaattat tggttgaatt gagaaagcac aagggtatga ctccagatag agctttggat 1560
actttacacg gtgatatgaa cttctacgcc actatgatga ttgctgctgg tgatgctgat 1620
ggtatggttt ctggtgcttg tcatacaact gcttctactg ttagaccagc tatgcaagtt 1680
ttgaagtctg ctgattctcc attggtcagt tccgttttca ttatgtgttt gccagacaga 1740
gttgttgttt atggtgattg tgccgttaat gttaatccaa ctgctgctga attagctacc 1800
attgctatta cttcagcaga tacagctgct gcttttggta ttgaacctag agttgctatg 1860
ttgtcctatt ccactttggg ttctggtact ggtccagatg ttttgaaagt tgaagaagct 1920
gttgctttgg ctaaagctag aagaccagat ttgaagatcg aaggtccaat tcaatacgat 1980
gctgctattg atccaaaagt tgctgctgtt aaggttactg gtggttctga agttgctggt 2040
aaagctactg tttttgtttt cccagacttg aacactggta acaacactta caaagccgtc 2100
caacaatcat caggtgctat tgctatgggt ccaatcatgc aagttccagt ttggggtggt 2160
tggccatatt tgccatggcc aggtttgttg aaaccagtta atgatttgtc tagaggttgc 2220
accgttccag atatcgttaa cactatttgc gttacctcca ttcaagcctc tagattgaga 2280
agaggtaata gacaatccgc tgattctact ccaactcaat ctatggatgg tggtcctggt 2340
ccatctaatg gtaacggtaa tggtaatggt aacggttctg gtgttattcc accacaattg 2400
gctatcgtct aa 2412
<210> 73
<211> 323
<212> PRT
<213> Bacillus subtilis
<400> 73
Met Ala Asp Leu Phe Ser Thr Val Gln Glu Lys Val Ala Gly Lys Asp
1 5 10 15
Val Lys Ile Val Phe Pro Glu Gly Leu Asp Glu Arg Ile Leu Glu Ala
20 25 30
Val Ser Lys Leu Ala Gly Asn Lys Val Leu Asn Pro Ile Val Ile Gly
35 40 45
Asn Glu Asn Glu Ile Gln Ala Lys Ala Lys Glu Leu Asn Leu Thr Leu
50 55 60
Gly Gly Val Lys Ile Tyr Asp Pro His Thr Tyr Glu Gly Met Glu Asp
65 70 75 80
Leu Val Gln Ala Phe Val Glu Arg Arg Lys Gly Lys Ala Thr Glu Glu
85 90 95
Gln Ala Arg Lys Ala Leu Leu Asp Glu Asn Tyr Phe Gly Thr Met Leu
100 105 110
Val Tyr Lys Gly Leu Ala Asp Gly Leu Val Ser Gly Ala Ala His Ser
115 120 125
Thr Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys Glu
130 135 140
Gly Val Lys Lys Thr Ser Gly Val Phe Ile Met Ala Arg Gly Glu Glu
145 150 155 160
Gln Tyr Val Phe Ala Asp Cys Ala Ile Asn Ile Ala Pro Asp Ser Gln
165 170 175
Asp Leu Ala Glu Ile Ala Ile Glu Ser Ala Asn Thr Ala Lys Met Phe
180 185 190
Asp Ile Glu Pro Arg Val Ala Met Leu Ser Phe Ser Thr Lys Gly Ser
195 200 205
Ala Lys Ser Asp Glu Thr Glu Lys Val Ala Asp Ala Val Lys Ile Ala
210 215 220
Lys Glu Lys Ala Pro Glu Leu Thr Leu Asp Gly Glu Phe Gln Phe Asp
225 230 235 240
Ala Ala Phe Val Pro Ser Val Ala Glu Lys Lys Ala Pro Asp Ser Glu
245 250 255
Ile Lys Gly Asp Ala Asn Val Phe Val Phe Pro Ser Leu Glu Ala Gly
260 265 270
Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Asn Phe Glu Ala Val
275 280 285
Gly Pro Ile Leu Gln Gly Leu Asn Met Pro Val Asn Asp Leu Ser Arg
290 295 300
Gly Cys Asn Ala Glu Asp Val Tyr Asn Leu Ala Leu Ile Thr Ala Ala
305 310 315 320
Gln Ala Leu
<210> 74
<211> 972
<212> DNA
<213> Bacillus subtilis
<400> 74
atggccgatt tgttctctac cgttcaagaa aaagttgctg gtaaggatgt caagatcgtt 60
tttccagaag gtttggacga aagaattttg gaagctgttt ctaaattggc cggtaacaag 120
gttttgaacc caatcgttat tggtaacgaa aacgaaattc aagccaaggc caaagaattg 180
aacttgactt tgggtggtgt taagatctac gatccacata cttatgaagg tatggaagat 240
ttggttcaag ccttcgttga aagaagaaaa ggtaaggcta ctgaagaaca agctagaaaa 300
gctttgttag acgaaaacta cttcggtact atgttggtct acaaaggttt ggctgatggt 360
ttggtttctg gtgctgctca ttctactgct gatactgtta gaccagcatt gcaaatcatc 420
aagacaaaag aaggtgtcaa aaagacctcc ggtgttttca ttatggctag aggtgaagaa 480
caatacgttt tcgctgattg cgctattaac attgctccag attctcaaga tttggccgaa 540
attgctattg aatctgctaa cactgctaag atgttcgaca ttgaacctag agttgctatg 600
ttgtcattct ctacaaaagg ttctgctaag tctgacgaaa ctgaaaaggt tgctgatgca 660
gttaagatcg ctaaagaaaa agctccagaa ttgaccttgg atggtgaatt tcaatttgat 720
gctgctttcg ttccatccgt tgctgaaaaa aaagcaccag attctgaaat caagggtgat 780
gccaatgttt tcgtattccc atctttagaa gctggtaaca tcggttacaa gattgctcaa 840
agattgggta actttgaagc tgttggtcca atattgcaag gtttgaatat gccagttaac 900
gatttgtcta gaggttgcaa tgcagaagat gtttacaact tggctttgat tactgctgct 960
caagctttgt aa 972
<210> 75
<211> 328
<212> PRT
<213> Thermoanaerobacterium saccharolyticum
<400> 75
Met Ser Ile Ile Gln Asn Ile Ile Glu Lys Ala Lys Ser Asp Lys Lys
1 5 10 15
Lys Ile Val Leu Pro Glu Gly Ala Glu Pro Arg Thr Leu Lys Ala Ala
20 25 30
Glu Ile Val Leu Lys Glu Gly Ile Ala Asp Leu Val Leu Leu Gly Asn
35 40 45
Glu Asp Glu Ile Arg Asn Ala Ala Lys Asp Leu Asp Ile Ser Lys Ala
50 55 60
Glu Ile Ile Asp Pro Val Lys Ser Glu Met Phe Asp Arg Tyr Ala Asn
65 70 75 80
Asp Phe Tyr Glu Leu Arg Lys Asn Lys Gly Ile Thr Leu Glu Lys Ala
85 90 95
Arg Glu Thr Ile Lys Asp Asn Ile Tyr Phe Gly Cys Met Met Val Lys
100 105 110
Glu Gly Tyr Ala Asp Gly Leu Val Ser Gly Ala Ile His Ala Thr Ala
115 120 125
Asp Leu Leu Arg Pro Ala Phe Gln Ile Ile Lys Thr Ala Pro Gly Ala
130 135 140
Lys Ile Val Ser Ser Phe Phe Ile Met Glu Val Pro Asn Cys Glu Tyr
145 150 155 160
Gly Glu Asn Gly Val Phe Leu Phe Ala Asp Cys Ala Val Asn Pro Ser
165 170 175
Pro Asn Ala Glu Glu Leu Ala Ser Ile Ala Val Gln Ser Ala Asn Thr
180 185 190
Ala Lys Asn Leu Leu Gly Phe Glu Pro Lys Val Ala Met Leu Ser Phe
195 200 205
Ser Thr Lys Gly Ser Ala Ser His Glu Leu Val Asp Lys Val Arg Lys
210 215 220
Ala Thr Glu Ile Ala Lys Glu Leu Met Pro Asp Val Ala Ile Asp Gly
225 230 235 240
Glu Leu Gln Leu Asp Ala Ala Leu Val Lys Glu Val Ala Glu Leu Lys
245 250 255
Ala Pro Gly Ser Lys Val Ala Gly Cys Ala Asn Val Leu Ile Phe Pro
260 265 270
Asp Leu Gln Ala Gly Asn Ile Gly Tyr Lys Leu Val Gln Arg Leu Ala
275 280 285
Lys Ala Asn Ala Ile Gly Pro Ile Thr Gln Gly Met Gly Ala Pro Val
290 295 300
Asn Asp Leu Ser Arg Gly Cys Ser Tyr Arg Asp Ile Val Asp Val Ile
305 310 315 320
Ala Thr Thr Ala Val Gln Ala Gln
325
<210> 76
<211> 987
<212> DNA
<213> Thermoanaerobacterium saccharolyticum
<400> 76
atgtccatca tccaaaacat catcgaaaag gccaagtccg ataagaagaa aatcgttttg 60
ccagaaggtg ctgaacctag aactttgaaa gctgctgaaa tcgtcttgaa agaaggtatt 120
gctgatttgg tcttgttggg taacgaagac gaaattagaa atgctgccaa ggatttggat 180
atctccaagg ccgaaattat cgatccagtt aagtctgaaa tgttcgacag atacgctaac 240
gacttctacg aattgagaaa gaacaagggt atcaccttgg aaaaggctag agaaaccatt 300
aaggacaaca tctacttcgg ttgcatgatg gtcaaagaag gttacgctga cggtttggtt 360
tctggtgcta ttcatgctac agctgatttg ttaagaccag ccttccaaat tatcaaaact 420
gctccaggtg ctaagatcgt cagttcattt ttcattatgg aagtcccaaa ctgcgaatac 480
ggtgaaaatg gtgttttttt gttcgctgat tgtgccgtta atccatctcc aaatgctgaa 540
gaattggctt ccattgctgt tcaatctgct aatactgcta agaatttgtt gggtttcgaa 600
cctaaggttg ccatgttgtc tttttcaaca aaaggttccg cttcccatga attggttgat 660
aaggttagaa aggctaccga aatcgccaaa gaattgatgc cagatgttgc tattgatggt 720
gaattacaat tggatgctgc cttggtaaaa gaagttgctg aattgaaagc tccaggttca 780
aaagttgctg gttgtgctaa tgttttgatc ttcccagact tacaagctgg taacattggt 840
tacaagttgg ttcaaagatt ggctaaggct aatgccattg gtccaattac tcaaggtatg 900
ggtgctccag ttaatgattt gtctagaggt tgttcctaca gagatatcgt tgatgttatt 960
gctactaccg ctgttcaagc tcaataa 987
<210> 77
<211> 733
<212> PRT
<213> Aphanomyces astaci
<400> 77
Met Ser His Gln Phe Thr Thr Lys Ser Val Ala Ser Gln Ser Thr Met
1 5 10 15
Leu Arg Val Arg Pro Phe Leu Ser Ser Arg Lys Ala Ala Ile Thr Leu
20 25 30
Leu Pro Arg Ala Thr Thr Ser Arg Phe Phe Thr Asp Asp Ala Thr Lys
35 40 45
Lys Asn Asp Arg Leu Leu Val Met Thr Asn Gly Gly Val Ala Lys His
50 55 60
Ser His Leu Leu Leu Gly Leu Met Asn Lys Leu Ser Tyr Thr Phe Pro
65 70 75 80
Ser Val Gly Tyr Phe Arg Pro Val Ala Pro Asn Phe His Ser Thr His
85 90 95
Gly Asp His His Val Asp Leu Ile Arg Ser Glu Phe Lys Ile Lys Asp
100 105 110
Glu Pro Tyr Gln Leu Val Gly Met Thr Gln Ala Asp Ile Thr His Ala
115 120 125
His Leu Glu Gly Asp Thr Asp Ser Val Ile Asp Thr Met Leu Ser Lys
130 135 140
Phe Glu Tyr Leu Arg Glu Lys His Asp Phe Val Val Met Glu Gly Ala
145 150 155 160
Val Leu Asp Thr Ser Pro Glu Leu Ser Trp Glu Leu Asn Val Asp Ile
165 170 175
Ala Lys Ser Leu Asn Ala Pro Val Leu Leu Thr Val Asp Ala Asp Asp
180 185 190
Leu Thr Val Asp Pro Ala Leu His Trp Thr Ala Ala Glu Ser Val Ala
195 200 205
Trp Leu Ala Asp Gln Ile Thr Thr Arg Val Leu Leu Ala Lys Asp Met
210 215 220
Ala His Ala Glu Gly Leu Thr His Val Gly Thr Ile Val Asn Arg Val
225 230 235 240
Lys Thr Asp Asp Ala Leu Glu Leu Arg Asp Leu Val His Ala Gln Ile
245 250 255
Lys Ala Arg Gly Phe Asp Pro Thr Lys Leu Leu Gly Ile Leu Pro Leu
260 265 270
Asp Pro Val Leu Asn Ser Lys Arg Leu Asn Glu Val Val Ala Gln Leu
275 280 285
His Ala Lys Gln Leu Tyr Gly Asn Pro Met Ser Asn Ser Val Val Val
290 295 300
Thr Asp Gly Leu Met Ala Thr Thr Glu Leu Lys Asp Leu Phe Lys His
305 310 315 320
Ile Asn Lys His Asp Asp Gly Leu Leu Val Ile Val Ser Ser Glu Arg
325 330 335
Thr Asp Val Ile Leu Gly Leu Leu Ala Ser Arg Leu Ser Gly Ala Leu
340 345 350
Pro Gln Ile Ser Gly Ile Ile Leu Thr Asn Gly Gly Ile Pro Gln Asn
355 360 365
Glu Cys Gln Asp Ile Leu Ile Gly Leu Ala Gln Ile Asp Lys Ala Ser
370 375 380
Val Pro Ile Tyr Ser Val Glu Leu Asp Ser Tyr Arg Thr Ala Ile Ala
385 390 395 400
Leu Ser Lys Val Thr Cys Asp Ile Leu Pro Thr Ser Gln Asn Lys Ile
405 410 415
Gln Gln Ala Tyr Ile Leu Phe Asp Thr Asn Val Glu Ser Asp Glu Leu
420 425 430
Leu Ser His Leu Ile Glu Arg Thr Gly Gly His Gly Arg Thr Pro Lys
435 440 445
Gln Phe Lys His Phe Leu Phe Glu Ala Ser Arg Lys Ala Asp Gln His
450 455 460
Ile Val Leu Thr Glu Gly Glu Asp Asp Arg Ile Leu Gln Ala Ala Asp
465 470 475 480
Glu Val Leu Arg Arg Gly Ile Ala Arg Leu Thr Ile Leu Gly Asp Val
485 490 495
Glu Ser Ile Asn Ala Arg Ala Lys Thr Leu Arg Leu Asp Leu Ser Gln
500 505 510
Ala Thr Leu Leu Asp Pro Ser Lys Ala Asp Lys Leu Ala Thr Tyr Ala
515 520 525
Asp His Tyr Phe Glu Lys Arg Lys Ser Lys Gly Ile Thr Pro Glu Leu
530 535 540
Ala Lys Glu Thr Val Gly Glu Ala Thr Tyr Phe Gly Thr Val Met Val
545 550 555 560
Asp Leu Asp Asp Ala Asp Gly Met Val Ser Gly Val Cys His Thr Thr
565 570 575
Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile Lys Thr Arg Pro Asp
580 585 590
Ile Pro Leu Val Ser Ser Val Phe Phe Met Cys Leu Glu His Asp Val
595 600 605
Val Leu Tyr Gly Asp Cys Ala Val Asn Thr Asp Pro Thr Ala Gln Gln
610 615 620
Leu Ala Gln Ile Ala Val Gln Ser Ala Glu Ser Ala Val Ala Phe Gly
625 630 635 640
Ile Glu Pro Arg Val Ala Leu Leu Ser Tyr Ala Thr Gly Asp Ser Asn
645 650 655
Lys Gly Pro Ile Ile Asp Lys Val Arg Glu Ala Thr Lys Leu Ala Gln
660 665 670
Ser Met Ala Pro Gly Val Ser Ile Tyr Gly Pro Ile Gln Tyr Asp Ala
675 680 685
Ala Thr Asn Pro Ser Ile Ala Lys Gln Lys Val Lys Gly Leu Lys Gln
690 695 700
Ser Glu Met Glu Val Ala Gly His Ala Asn Val Leu Val Phe Pro Asp
705 710 715 720
Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Arg Val
725 730
<210> 78
<211> 2202
<212> DNA
<213> Aphanomyces astaci
<400> 78
atgtcccacc agttcaccac caagtccgtc gcctcccagt ccaccatgct gcgagtccga 60
cccttcctgt cctcccgaaa ggccgccatc accctgctgc cccgagccac cacctcccga 120
ttcttcaccg acgacgccac caagaagaac gaccgactgc tggtcatgac caacggcggc 180
gtcgccaagc actcccacct gctgctgggc ctgatgaaca agctgtccta caccttcccc 240
tccgtcggct acttccgacc cgtcgccccc aacttccact ccacccacgg cgaccaccac 300
gtcgacctga tccgatccga gttcaagatc aaggacgagc cctaccagct ggtcggcatg 360
acccaggccg acatcaccca cgcccacctg gagggcgaca ccgactccgt catcgacacc 420
atgctgtcca agttcgagta cctgcgagag aagcacgact tcgtcgtcat ggagggcgcc 480
gtcctggaca cctcccccga gctgtcctgg gagctgaacg tcgacatcgc caagtccctg 540
aacgcccccg tcctgctgac cgtcgacgcc gacgacctga ccgtcgaccc cgccctgcac 600
tggaccgccg ccgagtccgt cgcctggctg gccgaccaga tcaccacccg agtcctgctg 660
gccaaggaca tggcccacgc cgagggcctg acccacgtcg gcaccatcgt caaccgagtc 720
aagaccgacg acgccctgga gctgcgagac ctggtccacg cccagatcaa ggcccgaggc 780
ttcgacccca ccaagctgct gggcatcctg cccctggacc ccgtcctgaa ctccaagcga 840
ctgaacgagg tcgtcgccca gctgcacgcc aagcagctgt acggcaaccc catgtccaac 900
tccgtcgtcg tcaccgacgg cctgatggcc accaccgagc tgaaggacct gttcaagcac 960
atcaacaagc acgacgacgg cctgctggtc atcgtctcct ccgagcgaac cgacgtcatc 1020
ctgggcctgc tggcctcccg actgtccggc gccctgcccc agatctccgg catcatcctg 1080
accaacggcg gcatccccca gaacgagtgc caggacatcc tgatcggcct ggcccagatc 1140
gacaaggcct ccgtccccat ctactccgtc gagctggact cctaccgaac cgccatcgcc 1200
ctgtccaagg tcacctgcga catcctgccc acctcccaga acaagatcca gcaggcctac 1260
atcctgttcg acaccaacgt cgagtccgac gagctgctgt cccacctgat cgagcgaacc 1320
ggcggccacg gccgaacccc caagcagttc aagcacttcc tgttcgaggc ctcccgaaag 1380
gccgaccagc acatcgtcct gaccgagggc gaggacgacc gaatcctgca ggccgccgac 1440
gaggtcctgc gacgaggcat cgcccgactg accatcctgg gcgacgtcga gtccatcaac 1500
gcccgagcca agaccctgcg actggacctg tcccaggcca ccctgctgga cccctccaag 1560
gccgacaagc tggccaccta cgccgaccac tacttcgaga agcgaaagtc caagggcatc 1620
acccccgagc tggccaagga gaccgtcggc gaggccacct acttcggcac cgtcatggtc 1680
gacctggacg acgccgacgg catggtctcc ggcgtctgcc acaccaccgc caacaccatc 1740
cgacccgccc tgcagctgat caagacccga cccgacatcc ccctggtctc ctccgtcttc 1800
ttcatgtgcc tggagcacga cgtcgtcctg tacggcgact gcgccgtcaa caccgacccc 1860
accgcccagc agctggccca gatcgccgtc cagtccgccg agtccgccgt cgccttcggc 1920
atcgagcccc gagtcgccct gctgtcctac gccaccggcg actccaacaa gggccccatc 1980
atcgacaagg tccgagaggc caccaagctg gcccagtcca tggcccccgg cgtctccatc 2040
tacggcccca tccagtacga cgccgccacc aacccctcca tcgccaagca gaaggtcaag 2100
ggcctgaagc agtccgagat ggaggtcgcc ggccacgcca acgtcctggt cttccccgac 2160
ctgaacaccg gcaacaacac ctacaaggcc gtccgagtct aa 2202
<210> 79
<211> 792
<212> PRT
<213> Chlamydomonas reinhardtii
<400> 79
Met Ser Leu Asn Ser Ser Thr Met Ser Arg Arg Gln Ala Val Ala Gly
1 5 10 15
Ala Pro Ala Val Ala Pro Phe Arg His Ala Gly Leu Phe Pro Arg Val
20 25 30
Arg Leu Cys Ala Asn Arg Arg Val Ala Arg Val Ala Pro Lys Ala Ala
35 40 45
Gly Asn Gly Asn Ile Ala Gln Gly Glu Gln Gly Phe Asp Thr Leu Phe
50 55 60
Leu Ser Asp Ile Ser Leu Val Gly Gln Arg Thr Pro Leu Leu Leu Gly
65 70 75 80
Phe Phe Asn Tyr Phe Glu Arg His Leu Pro His Val Gly Phe Phe Glu
85 90 95
Pro Ile Ala Ala Glu Ala Leu Ala Ser Ser Glu Leu Arg Ile Asp Arg
100 105 110
His Val Glu Leu Val Tyr Lys Val Phe Asn Leu Lys Gly Asp Val Arg
115 120 125
Ala Met Thr Gly Val Gln Asp Ala Glu Ala Ala Arg Met Ile Ala Asn
130 135 140
Gly Gln His Ser Glu Leu Leu Asp Lys Ile Tyr Ser Gln Tyr Ala Ser
145 150 155 160
Tyr Lys Glu Gly Gln Asp Leu Val Leu Val Glu Gly Pro Gly Pro Leu
165 170 175
Met Gly Gly Thr Glu Leu Asp Ala Gln Ile Ala Ala Ala Leu Asn Ala
180 185 190
Pro Val Leu Met Thr Met Thr Gly Gln Pro Asn Ala Thr Val Ala Asp
195 200 205
Tyr Tyr Asn Arg Ala Met Val Lys Arg Gln Val Phe Leu Asp His His
210 215 220
Val Glu Val Leu Gly Leu Val Met Asn Gly Leu Pro Arg Gln Ser His
225 230 235 240
Ala Ile Leu Ser Gly Gln Leu Arg Asp Lys Phe Ala Ala Ala Gly Leu
245 250 255
Pro Phe Ala Gly Ala Ile Pro Thr Asp Ile Met Leu Arg Asn Val Arg
260 265 270
Leu Asp Glu Val Gln Thr Ala Met Gly Ala Gln Arg Leu Tyr Gly Asp
275 280 285
Ser Leu Leu Thr Asp Val Glu Phe Asp Asp Val Val Val Ala Ser Gln
290 295 300
Arg Leu Glu Glu Leu Leu Glu Ile Leu Ala Glu Arg Pro Met Gly Arg
305 310 315 320
Pro Leu Val Val Thr Ser Ala Asp Arg Leu Asp Ile Val Leu Gly Leu
325 330 335
Leu Ala Ala Gln Leu Ser Val Ser Gly Pro Gly Val Ala Gly Ile Leu
340 345 350
Leu Thr Gln Ala Gly Ser Ala Arg Ser Gly Arg Asn Tyr Ala Arg Asp
355 360 365
Thr Ile Asp Arg Ile Phe Ala Gly Leu Ser Ser Ser Gly Leu Tyr Lys
370 375 380
Gly Ser Leu Leu Pro Val Leu Val Thr Asp Met Pro Leu Arg Asp Ala
385 390 395 400
Ile Arg Lys Leu Asp Asn Leu Asp Ala Ala Ile Leu Pro Ser Ser Thr
405 410 415
Arg Lys Ile Ser Gln Cys Lys Arg Leu Phe Glu Gln Tyr Val Asp Ala
420 425 430
Asn Ala Val Val Ala Arg Leu Gln Asn Met Val Arg Pro Asn Arg Met
435 440 445
Thr Pro Lys Met Phe Met His Thr Leu Lys Ser Met Cys Asn Ala Thr
450 455 460
Pro Gln His Ile Val Leu Pro Glu Ser Glu Asp Lys Arg Val Leu Ala
465 470 475 480
Ala Ala Ala Asp Val Val Gln Arg Gly Leu Ala Lys Ile Thr Leu Leu
485 490 495
Gly Asp Pro Thr Thr Ile Leu Ala Glu Ala Ala Lys Leu Gly Leu Asp
500 505 510
Leu Ser Gly Cys Asn Ile His Asn Pro Asn Thr Ser Asp Arg Phe Asp
515 520 525
Lys Tyr Val Asp Met Leu Val Glu Ala Arg Lys Lys Lys Gly Met Thr
530 535 540
Arg Glu Val Ala Ala Asp Thr Leu His Gly Asp Val Asn Phe Phe Ala
545 550 555 560
Thr Met Met Ile Val Ala Gly Asp Ala Asp Gly Met Val Ser Gly Ala
565 570 575
Val His Thr Thr Ala Ser Thr Val Arg Pro Ala Leu Gln Val Leu Lys
580 585 590
Ser Pro Asp Thr Pro Leu Val Ser Ser Val Phe Ile Met Cys Leu Pro
595 600 605
Asp Arg Val Val Val Tyr Gly Asp Cys Ala Val Asn Val Asn Pro Ser
610 615 620
Ala Ala Asp Leu Ala Gln Ile Ala Ile Thr Ser Asn Asp Thr Ala Ala
625 630 635 640
Ala Phe Gly Ile Glu Pro Arg Val Ala Met Leu Ser Tyr Ser Thr Leu
645 650 655
Gly Ser Gly Ser Gly Pro Asp Val Gln Lys Val Ser Glu Ala Val Ala
660 665 670
Ile Val Lys Gln Arg Arg Pro Asp Ile Lys Val Glu Gly Pro Ile Gln
675 680 685
Tyr Asp Ala Ala Ile Asp Pro Lys Val Ala Ala Val Lys Val Gln Gly
690 695 700
Leu Ser Glu Val Ala Gly Lys Ala Thr Val Phe Ile Phe Pro Asp Leu
705 710 715 720
Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln Gln Ser Thr Gly Ala
725 730 735
Ile Ala Met Gly Pro Val Met Gln Gly Leu Leu Arg Pro Val Asn Asp
740 745 750
Leu Ser Arg Gly Cys Thr Val Pro Asp Ile Ile Asn Thr Ile Cys Val
755 760 765
Thr Ser Ile Gln Ala Ser Arg Met Ser Ser Ala Ala Arg Ala Ala Ala
770 775 780
Ala Lys Ala Ala Val Ala Ala Val
785 790
<210> 80
<211> 2379
<212> DNA
<213> Chlamydomonas reinhardtii
<400> 80
atgtccctga actcctccac catgtcccga cgacaggccg tcgccggcgc ccccgccgtc 60
gcccccttcc gacacgccgg cctgttcccc cgagtccgac tgtgcgccaa ccgacgagtc 120
gcccgagtcg cccccaaggc cgccggcaac ggcaacatcg cccagggcga gcagggcttc 180
gacaccctgt tcctgtccga catctccctg gtcggccagc gaacccccct gctgctgggc 240
ttcttcaact acttcgagcg acacctgccc cacgtcggct tcttcgagcc catcgccgcc 300
gaggccctgg cctcctccga gctgcgaatc gaccgacacg tcgagctggt ctacaaggtc 360
ttcaacctga agggcgacgt ccgagccatg accggcgtcc aggacgccga ggccgcccga 420
atgatcgcca acggccagca ctccgagctg ctggacaaga tctactccca gtacgcctcc 480
tacaaggagg gccaggacct ggtcctggtc gagggccccg gccccctgat gggcggcacc 540
gagctggacg cccagatcgc cgccgccctg aacgcccccg tcctgatgac catgaccggc 600
cagcccaacg ccaccgtcgc cgactactac aaccgagcca tggtcaagcg acaggtcttc 660
ctggaccacc acgtcgaggt cctgggcctg gtcatgaacg gcctgccccg acagtcccac 720
gccatcctgt ccggccagct gcgagacaag ttcgccgccg ccggcctgcc cttcgccggc 780
gccatcccca ccgacatcat gctgcgaaac gtccgactgg acgaggtcca gaccgccatg 840
ggcgcccagc gactgtacgg cgactccctg ctgaccgacg tcgagttcga cgacgtcgtc 900
gtcgcctccc agcgactgga ggagctgctg gagatcctgg ccgagcgacc catgggccga 960
cccctggtcg tcacctccgc cgaccgactg gacatcgtcc tgggcctgct ggccgcccag 1020
ctgtccgtct ccggccccgg cgtcgccggc atcctgctga cccaggccgg ctccgcccga 1080
tccggccgaa actacgcccg agacaccatc gaccgaatct tcgccggcct gtcctcctcc 1140
ggcctgtaca agggctccct gctgcccgtc ctggtcaccg acatgcccct gcgagacgcc 1200
atccgaaagc tggacaacct ggacgccgcc atcctgccct cctccacccg aaagatctcc 1260
cagtgcaagc gactgttcga gcagtacgtc gacgccaacg ccgtcgtcgc ccgactgcag 1320
aacatggtcc gacccaaccg aatgaccccc aagatgttca tgcacaccct gaagtccatg 1380
tgcaacgcca ccccccagca catcgtcctg cccgagtccg aggacaagcg agtcctggcc 1440
gccgccgccg acgtcgtcca gcgaggcctg gccaagatca ccctgctggg cgaccccacc 1500
accatcctgg ccgaggccgc caagctgggc ctggacctgt ccggctgcaa catccacaac 1560
cccaacacct ccgaccgatt cgacaagtac gtcgacatgc tggtcgaggc ccgaaagaag 1620
aagggcatga cccgagaggt cgccgccgac accctgcacg gcgacgtcaa cttcttcgcc 1680
accatgatga tcgtcgccgg cgacgccgac ggcatggtct ccggcgccgt ccacaccacc 1740
gcctccaccg tccgacccgc cctgcaggtc ctgaagtccc ccgacacccc cctggtctcc 1800
tccgtcttca tcatgtgcct gcccgaccga gtcgtcgtct acggcgactg cgccgtcaac 1860
gtcaacccct ccgccgccga cctggcccag atcgccatca cctccaacga caccgccgcc 1920
gccttcggca tcgagccccg agtcgccatg ctgtcctact ccaccctggg ctccggctcc 1980
ggccccgacg tccagaaggt ctccgaggcc gtcgccatcg tcaagcagcg acgacccgac 2040
atcaaggtcg agggccccat ccagtacgac gccgccatcg accccaaggt cgccgccgtc 2100
aaggtccagg gcctgtccga ggtcgccggc aaggccaccg tcttcatctt ccccgacctg 2160
aacaccggca acaacaccta caaggccgtc cagcagtcca ccggcgccat cgccatgggc 2220
cccgtcatgc agggcctgct gcgacccgtc aacgacctgt cccgaggctg caccgtcccc 2280
gacatcatca acaccatctg cgtcacctcc atccaggcct cccgaatgtc ctccgccgcc 2340
cgagccgccg ccgccaaggc cgccgtcgcc gccgtctaa 2379
<210> 81
<211> 353
<212> PRT
<213> Perkinsus marinus
<400> 81
Met Leu Ser Arg Ser Thr Ala Arg Cys Ala Pro Ala Ala Leu Ala Gly
1 5 10 15
Ile Arg Gln Arg Ala Met Gln Thr Gly Leu Lys Lys Phe Ile Ala Phe
20 25 30
Pro Glu Val Ser Asp Glu Arg Val Ile Pro Ala Val Ala Lys Val Leu
35 40 45
Lys Glu Lys Ile Ala Gln Pro Val Leu Val Gly Asp Arg Glu Ala Ala
50 55 60
Tyr Lys Cys Ala Lys Ala Asn Asn Val Ser Leu Glu Gly Val Arg Ile
65 70 75 80
Ile Asp Pro Ala Leu His Pro Glu Val Val Glu Gln Thr Ala Thr Val
85 90 95
Leu Phe Gln Lys Arg Gln Lys Lys Gly Met Thr Ile Asp Ala Ala Leu
100 105 110
Asp Thr Val Lys Asn Ser Pro Leu Met Met Ala Asn Leu Met Leu Thr
115 120 125
Thr Gly His Val Gln Gly Cys Val Ala Gly Ala Ser His Thr Ser Ala
130 135 140
Asp Val Ala Arg Ala Ala Leu Gln Thr Val Gly Val Lys Lys Gly Leu
145 150 155 160
Lys Thr Ala Ser Ser Phe Phe Ile Ile Ala Lys Asp Asp Lys Thr Phe
165 170 175
Leu Phe Ser Asp Cys Gly Phe Cys Ile Ala Pro Ser Ile Ser Gln Leu
180 185 190
Ala Glu Ile Ala Ile Thr Thr Ala Gln Thr Cys Glu Asp Val Leu Ala
195 200 205
Ser Thr Pro Arg Ile Ala Met Leu Ser Phe Ser Thr Phe Gly Ser Ala
210 215 220
Lys His Glu Tyr Val Thr Arg Val Glu Glu Ala Leu Ala Leu Ala Arg
225 230 235 240
Lys Glu Arg Pro Asp Leu Ala Ile Asp Gly Glu Met Gln Val Asp Ala
245 250 255
Ala Ile Val Pro Glu Val Ala Ala Lys Lys Ala Pro Gly Ser Lys Val
260 265 270
Ala Gly His Ala Asn Val Leu Ile Phe Pro Asp Leu Asn Ala Gly Asn
275 280 285
Ile Ala Tyr Lys Val Ala Glu Arg Phe Gly Gly Tyr Gln Ala Val Gly
290 295 300
Pro Val Phe Gln Gly Leu Ala Tyr Pro Thr Asn Asp Leu Ser Arg Gly
305 310 315 320
Cys His Ala Glu Asp Val Val Asp Ala Ala Ala Val Thr Val Leu Gln
325 330 335
Gly Ala Ser Ile Pro Ile Pro Thr Gly Pro Ala Pro Gly Asp Val Leu
340 345 350
Asn
<210> 82
<211> 1062
<212> DNA
<213> Perkinsus marinus
<400> 82
atgctgtccc gatccaccgc ccgatgcgcc cccgccgccc tggccggcat ccgacagcga 60
gccatgcaga ccggcctgaa gaagttcatc gccttccccg aggtctccga cgagcgagtc 120
atccccgccg tcgccaaggt cctgaaggag aagatcgccc agcccgtcct ggtcggcgac 180
cgagaggccg cctacaagtg cgccaaggcc aacaacgtct ccctggaggg cgtccgaatc 240
atcgaccccg ccctgcaccc cgaggtcgtc gagcagaccg ccaccgtcct gttccagaag 300
cgacagaaga agggcatgac catcgacgcc gccctggaca ccgtcaagaa ctcccccctg 360
atgatggcca acctgatgct gaccaccggc cacgtccagg gctgcgtcgc cggcgcctcc 420
cacacctccg ccgacgtcgc ccgagccgcc ctgcagaccg tcggcgtcaa gaagggcctg 480
aagaccgcct cctccttctt catcatcgcc aaggacgaca agaccttcct gttctccgac 540
tgcggcttct gcatcgcccc ctccatctcc cagctggccg agatcgccat caccaccgcc 600
cagacctgcg aggacgtcct ggcctccacc ccccgaatcg ccatgctgtc cttctccacc 660
ttcggctccg ccaagcacga gtacgtcacc cgagtcgagg aggccctggc cctggcccga 720
aaggagcgac ccgacctggc catcgacggc gagatgcagg tcgacgccgc catcgtcccc 780
gaggtcgccg ccaagaaggc ccccggctcc aaggtcgccg gccacgccaa cgtcctgatc 840
ttccccgacc tgaacgccgg caacatcgcc tacaaggtcg ccgagcgatt cggcggctac 900
caggccgtcg gccccgtctt ccagggcctg gcctacccca ccaacgacct gtcccgaggc 960
tgccacgccg aggacgtcgt cgacgccgcc gccgtcaccg tcctgcaggg cgcctccatc 1020
cccatcccca ccggccccgc ccccggcgac gtcctgaact aa 1062
<210> 83
<211> 297
<212> PRT
<213> Perkinsus marinus
<400> 83
Val Gly Gly Arg Pro Arg Gly Asn Tyr Lys Cys Ala Lys Ala Asn Asn
1 5 10 15
Val Ser Leu Glu Gly Val Arg Ile Ile Asp Pro Ala Leu His Pro Glu
20 25 30
Val Val Glu Gln Thr Ala Thr Val Leu Phe Gln Lys Arg Gln Lys Lys
35 40 45
Gly Met Thr Ile Asp Ala Ala Leu Asp Thr Val Lys Asn Ser Pro Leu
50 55 60
Met Met Ala Asn Leu Met Leu Thr Thr Gly His Val Gln Gly Cys Val
65 70 75 80
Ala Gly Ala Ser His Thr Ser Ala Asp Val Ala Arg Ala Ala Leu Gln
85 90 95
Thr Val Gly Val Lys Lys Gly Leu Lys Thr Ala Ser Ser Phe Phe Ile
100 105 110
Ile Ala Lys Asp Asp Lys Thr Phe Leu Phe Ser Asp Cys Gly Phe Cys
115 120 125
Ile Ala Pro Ser Ile Ser Gln Leu Ala Glu Ile Ala Ile Thr Thr Ala
130 135 140
Gln Thr Cys Glu Asp Val Leu Ala Ser Thr Pro Arg Ile Ala Met Leu
145 150 155 160
Ser Phe Ser Thr Phe Gly Ser Ala Lys His Glu Tyr Val Thr Arg Val
165 170 175
Glu Glu Ala Leu Ala Leu Ala Arg Lys Glu Arg Pro Asp Leu Ala Ile
180 185 190
Asp Gly Glu Met Gln Val Asp Ala Ala Ile Val Pro Glu Val Ala Ala
195 200 205
Lys Lys Ala Pro Gly Ser Lys Val Ala Gly Gln Ala Asn Val Leu Ile
210 215 220
Phe Pro Asp Leu Asn Ala Gly Asn Ile Ala Tyr Lys Val Ala Glu Arg
225 230 235 240
Phe Gly Gly Tyr Gln Ala Val Gly Pro Val Phe Gln Gly Leu Ala Tyr
245 250 255
Pro Thr Asn Asp Leu Ser Arg Gly Cys His Ala Glu Asp Val Val Asp
260 265 270
Ala Ala Ala Val Thr Val Leu Gln Gly Ala Ser Ile Pro Ile Pro Thr
275 280 285
Gly Pro Ala Pro Gly Asp Val Leu Asn
290 295
<210> 84
<211> 894
<212> DNA
<213> Perkinsus marinus
<400> 84
gtcggcggcc gaccccgagg caactacaag tgcgccaagg ccaacaacgt ctccctggag 60
ggcgtccgaa tcatcgaccc cgccctgcac cccgaggtcg tcgagcagac cgccaccgtc 120
ctgttccaga agcgacagaa gaagggcatg accatcgacg ccgccctgga caccgtcaag 180
aactcccccc tgatgatggc caacctgatg ctgaccaccg gccacgtcca gggctgcgtc 240
gccggcgcct cccacacctc cgccgacgtc gcccgagccg ccctgcagac cgtcggcgtc 300
aagaagggcc tgaagaccgc ctcctccttc ttcatcatcg ccaaggacga caagaccttc 360
ctgttctccg actgcggctt ctgcatcgcc ccctccatct cccagctggc cgagatcgcc 420
atcaccaccg cccagacctg cgaggacgtc ctggcctcca ccccccgaat cgccatgctg 480
tccttctcca ccttcggctc cgccaagcac gagtacgtca cccgagtcga ggaggccctg 540
gccctggccc gaaaggagcg acccgacctg gccatcgacg gcgagatgca ggtcgacgcc 600
gccatcgtcc ccgaggtcgc cgccaagaag gcccccggct ccaaggtcgc cggccaggcc 660
aacgtcctga tcttccccga cctgaacgcc ggcaacatcg cctacaaggt cgccgagcga 720
ttcggcggct accaggccgt cggccccgtc ttccagggcc tggcctaccc caccaacgac 780
ctgtcccgag gctgccacgc cgaggacgtc gtcgacgccg ccgccgtcac cgtcctgcag 840
ggcgcctcca tccccatccc caccggcccc gcccccggcg acgtcctgaa ctaa 894
<210> 85
<211> 219
<212> PRT
<213> Perkinsus marinus
<400> 85
Val Ser Pro Gly Ala Ser His Thr Ser Ala Asp Val Ala Arg Ala Ala
1 5 10 15
Leu Gln Thr Val Gly Val Lys Lys Gly Leu Lys Thr Ala Ser Ser Phe
20 25 30
Phe Ile Ile Ala Lys Asp Asp Lys Thr Phe Leu Phe Ser Asp Cys Gly
35 40 45
Phe Cys Ile Ala Pro Ser Ile Ser Gln Leu Ala Glu Ile Ala Ile Thr
50 55 60
Thr Ala Gln Thr Cys Glu Asp Val Leu Ala Thr Thr Pro Arg Val Ala
65 70 75 80
Met Leu Ser Phe Ser Thr Phe Gly Ser Ala Lys His Glu Tyr Val Thr
85 90 95
Arg Val Glu Glu Ala Leu Ala Leu Ala Arg Lys Glu Lys Pro Asp Leu
100 105 110
Ala Ile Asp Gly Glu Met Gln Val Asp Ala Ala Ile Val Pro Glu Val
115 120 125
Ala Ala Lys Lys Ala Pro Gly Ser Lys Val Ala Gly His Ala Asn Val
130 135 140
Leu Ile Phe Pro Asp Leu Asn Ala Gly Asn Ile Ala Tyr Lys Val Ala
145 150 155 160
Glu Arg Phe Gly Gly Tyr Gln Ala Val Gly Pro Ile Phe Gln Gly Leu
165 170 175
Ala Tyr Pro Thr Asn Asp Leu Ser Arg Gly Cys His Ala Glu Asp Val
180 185 190
Val Asp Ala Ala Ala Val Thr Val Leu Gln Gly Ser Ser Ile Pro Ile
195 200 205
Pro Thr Gly Pro Ala Pro Gly Asp Ile Leu Asn
210 215
<210> 86
<211> 660
<212> DNA
<213> Perkinsus marinus
<400> 86
gtctcccccg gcgcctccca cacctccgcc gacgtcgccc gagccgccct gcagaccgtc 60
ggcgtcaaga agggcctgaa gaccgcctcc tccttcttca tcatcgccaa ggacgacaag 120
accttcctgt tctccgactg cggcttctgc atcgccccct ccatctccca gctggccgag 180
atcgccatca ccaccgccca gacctgcgag gacgtcctgg ccaccacccc ccgagtcgcc 240
atgctgtcct tctccacctt cggctccgcc aagcacgagt acgtcacccg agtcgaggag 300
gccctggccc tggcccgaaa ggagaagccc gacctggcca tcgacggcga gatgcaggtc 360
gacgccgcca tcgtccccga ggtcgccgcc aagaaggccc ccggctccaa ggtcgccggc 420
cacgccaacg tcctgatctt ccccgacctg aacgccggca acatcgccta caaggtcgcc 480
gagcgattcg gcggctacca ggccgtcggc cccatcttcc agggcctggc ctaccccacc 540
aacgacctgt cccgaggctg ccacgccgag gacgtcgtcg acgccgccgc cgtcaccgtc 600
ctgcagggct cctccatccc catccccacc ggccccgccc ccggcgacat cctgaactaa 660
<210> 87
<211> 771
<212> PRT
<213> Phytophthora infestans
<400> 87
Met Trp Thr Leu Arg His Ser Leu Arg Arg Ser Val Gly Met Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Thr
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys His Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Ile Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Glu Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Val Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ser Ser Asn Ala Leu Leu Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Ala Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Lys Asp Val Ala Thr Leu Arg
210 215 220
Glu Asp Leu Lys Leu Lys Met Gly Lys Lys Glu Ile Pro Phe Leu Gly
225 230 235 240
Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu Val
245 250 255
Thr His Gln Leu Gly Ala Lys Gln Leu Phe Gly Asn Ser Ile Ala Asn
260 265 270
Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile Thr
290 295 300
Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg Leu
305 310 315 320
Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly Asn
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Glu Ala Leu
340 345 350
Asp Lys Thr Gly Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp Thr
355 360 365
Asn Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Tyr Ile Leu Pro
370 375 380
Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Ser Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Arg Ile Thr
450 455 460
Ile Leu Gly Asp Pro Asp Glu Ile Thr Val Asn Ala Lys Met Ala Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Ile Arg Pro Val Asp Ser His Leu
485 490 495
Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys Arg Lys His Lys Gly
500 505 510
Val Thr Arg Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr Phe
515 520 525
Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Thr Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys
565 570 575
Leu Lys Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Met Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu
660 665 670
Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn Val Leu
675 680 685
Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln
690 695 700
Gln Ser Thr Gly Cys Ile Ala Met Gly Pro Met Leu Gln Gly Leu Arg
705 710 715 720
Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp Ile Val
725 730 735
Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile Leu Lys
740 745 750
Arg Glu Ala Glu Asn Ser Lys Asn Gln Asn Gly Glu Phe Leu Glu Ser
755 760 765
Ala Thr Met
770
<210> 88
<211> 2316
<212> DNA
<213> Phytophthora infestans
<400> 88
atgtggaccc tgcgacactc cctgcgacga tccgtcggca tggccctgcc ccaccgacga 60
gccctgaccg ccgccgccat ctcccagggc aaggtcccca tcaacaacct gtacgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctggccca cgtcctggag 180
cagaagttca ccaaggtcgg ctacttccga cccatccagc cctcccccga ctcctccatg 240
gccgaccacc acgtcgacgt catgaagcac cagctggagc tgtccaagga cgtcgaggag 300
ctgtacggcg tcacctcctc ccgagccatg gaggccatgc tgaccggcaa gggcgacgac 360
atcgtcgagg agatcctgga gcgatacgag gagtgccgaa agggccacga cttcatgatc 420
atcgagggct cccaggtctc caagcacgag tccgccatgt cctggaagat caacgtcgac 480
atcgccaagg ccatcggctc ccccgtcctg atggtcaccg acttcggcga cacctccgcc 540
tcctccaacg ccctgctgga ggagatggtc tcccgaaccg tcatgggccg agaccaggcc 600
gaggccgccg gcctgaacta cctgggcacc atcgccaacc gagtccgagc caaggacgtc 660
gccaccctgc gagaggacct gaagctgaag atgggcaaga aggagatccc cttcctgggc 720
ttcctgccca tggacgagat catcgcctcc aagcgactga acgaggtcac ccaccagctg 780
ggcgccaagc agctgttcgg caactccatc gccaacgacg ccgtcgtcac ctccgccgtc 840
gtcgccgcct ccgccctgaa ggacctgttc gcccacctga agaagtacaa ggacggcgcc 900
atgatcatca cctccggcga ccgatccgac ctgatgctgg gcctgatggt ctcccgactg 960
cccggcgtcc tgcccaacat ctccgccatc gtcctgacca acggcaacta cccccactcc 1020
aacacccagg agatcctgaa gggcgtcgag gccctggaca agaccggcct gtccctgccc 1080
atcttctcca cccccaacga caccaactcc accgccgacg gcttcgccaa ggtctccacc 1140
tacatcctgc cctcctccaa gctgaagatc gaccgatcca agcagctgtt cgacgagttc 1200
gtcgagaagg agatgctgat cggcgagctg gacgagggca tggtcgtctc ccgatccccc 1260
aagcagttcc agcacttcct gttctccaag tcccgagccg tccagcgaca catcgtcctg 1320
accgagggcg aggacatccg agtcctgcag gccgccgacc agatcctgcg acagaacctg 1380
tcccgaatca ccatcctggg cgaccccgac gagatcaccg tcaacgccaa gatggccaac 1440
ctggacctgt cccgagccaa catcatccga cccgtcgact cccacctgct ggacaagtac 1500
gtcgactact tctacgccaa gcgaaagcac aagggcgtca cccgagagct ggcccgagac 1560
tactgcaagg acgagaccta cttcggcacc ctgatggtcg agctgggcga cgccgacggc 1620
atggtctccg gcgcctgcca caccaccgcc aacaccatcc gacccgccct gcagctgatc 1680
aagaccaccc ccaaccgacc catcgtctcc tccatcttct tcatgtgcct gaaggacggc 1740
gtccgaatct acggcgactg cgccgtcaac accgacccct ccgcccagga cctggcccag 1800
atcgccgtca cctccgccga gtccgccgag gccttcggcc tgatccccaa ggtcgccctg 1860
ctgtcctacg ccaccggcga ctccaactcc ggccccatca tcgacaaggt ccgagaggcc 1920
accaagatgg cccaggagct gcgacccgac ctggacatct acggccccat ccagtacgac 1980
gccgccgtcg acgagtccat cgccaagacc aagctgaagg ccatcccctc cggcgccaag 2040
gtcggcggcc aggccaacgt cctgatcttc cccgacctga acaccggcaa caacacctac 2100
aaggccgtcc agcagtccac cggctgcatc gccatgggcc ccatgctgca gggcctgcga 2160
aagcccgtca acgacctgtc ccgaggcgcc accgtcaagg acatcgtcac caccgtcgcc 2220
atcaccgcca tccaggccga ccaggtcatc ctgaagcgag aggccgagaa ctccaagaac 2280
cagaacggcg agttcctgga gtccgccacc atgtaa 2316
<210> 89
<211> 760
<212> PRT
<213> Phytophthora parasitica
<400> 89
Met Trp Thr Leu Arg Arg Ser Leu Arg His Ser Thr Gly Val Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Thr
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ala Asn Asp Ala Leu Ile Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Asp Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Ser Asn Val Asp Ser Leu Arg
210 215 220
Ala Ser Leu Lys Arg Lys Met Gly Asp Lys Asp Ile Pro Phe Leu Gly
225 230 235 240
Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu Val
245 250 255
Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly His Ser Ile Ala Asn
260 265 270
Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile Thr
290 295 300
Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg Leu
305 310 315 320
Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly Asn
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Gln Ala Leu
340 345 350
Asp Lys Thr Ala Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp Thr
355 360 365
Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile Leu Pro
370 375 380
Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Lys Ile Thr
450 455 460
Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn Ala Lys Met Ala Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Val Ser Pro Ala Asn Ser Ala Leu
485 490 495
Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys Arg Lys His Lys Gly
500 505 510
Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr Phe
515 520 525
Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys
565 570 575
Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu
660 665 670
Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn Val Leu
675 680 685
Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln
690 695 700
Gln Ser Thr Gly Cys Ile Ala Met Gly Pro Met Leu Gln Gly Leu Arg
705 710 715 720
Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp Ile Val
725 730 735
Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile Met Lys
740 745 750
Arg Glu Ala Glu Asn Ala Thr Lys
755 760
<210> 90
<211> 2283
<212> DNA
<213> Phytophthora parasitica
<400> 90
atgtggaccc tgcgacgatc cctgcgacac tccaccggcg tcgccctgcc ccaccgacga 60
gccctgaccg ccgccgccat ctcccagggc aaggtcccca tcaacaacct gtacgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctggccca cgtcctggag 180
cagaagttca ccaaggtcgg ctacttccga cccatccagc cctcccccga ctcctccatg 240
gccgaccacc acgtcgacgt catgaagcag cagctggagc tgtccaagga cgtcgaggag 300
ctgtacggcg tcacctcctc ccgagccatg gaggccatgc tgaccggcaa gggcgacgac 360
gtcgtcgagg agatcctgga gcgatacgag cagtgccgaa agggccacga cttcatgatc 420
atcgagggct cccagatctc caagcacgag tccgccatgt cctggaagat caacgtcgac 480
atcgccaagg ccatcggctc ccccgtcctg atggtcaccg acttcggcga cacctccgcc 540
gccaacgacg ccctgatcga ggagatggtc tcccgaaccg tcatgggccg agaccaggcc 600
gaggacgccg gcctgaacta cctgggcacc atcgccaacc gagtccgagc ctccaacgtc 660
gactccctgc gagcctccct gaagcgaaag atgggcgaca aggacatccc cttcctgggc 720
ttcctgccca tggacgagat catcgcctcc aagcgactga acgaggtcac ccaccagctg 780
ggcgccaccc agctgttcgg ccactccatc gccaacgacg ccgtcgtcac ctccgccgtc 840
gtcgccgcct ccgccctgaa ggacctgttc gcccacctga agaagtacaa ggacggcgcc 900
atgatcatca cctccggcga ccgatccgac ctgatgctgg gcctgatggt ctcccgactg 960
cccggcgtcc tgcccaacat ctccgccatc gtcctgacca acggcaacta cccccactcc 1020
aacacccagg agatcctgaa gggcgtccag gccctggaca agaccgccct gtccctgccc 1080
atcttctcca cccccaacga caccttctcc accgccgacg gcttcgccaa ggtctccacc 1140
gacatcctgc cctcctccaa gctgaagatc gaccgatcca agcagctgtt cgacgagttc 1200
gtcgagaagg agatgctgat cggcgagctg gacgagggca tggtcgtcaa ccgatccccc 1260
aagcagttcc agcacttcct gttctccaag tcccgagccg tccagcgaca catcgtcctg 1320
accgagggcg aggacatccg agtcctgcag gccgccgacc agatcctgcg acagaacctg 1380
tccaagatca ccatcctggg cgaccccgac gagatcctgc tgaacgccaa gatggccaac 1440
ctggacctgt cccgagccaa catcgtctcc cccgccaact ccgccctgct ggacaagtac 1500
gtcgactact tctacgccaa gcgaaagcac aagggcgtca ccaaggagct ggcccgagac 1560
tactgcaagg acgagaccta cttcggcacc ctgatggtcg agctgggcga cgccgacggc 1620
atggtctccg gcgcctgcca caccaccgcc aacaccatcc gacccgccct gcagctgatc 1680
aagaccgccc ccaaccgacc catcgtctcc tccatcttct tcatgtgcct ggaggacggc 1740
gtccgaatct acggcgactg cgccgtcaac accgacccct ccgcccagga cctggcccag 1800
atcgccgtca cctccgccga gtccgccgag gccttcggcc tgatccccaa ggtcgccctg 1860
ctgtcctacg ccaccggcga ctccaactcc ggccccatca tcgacaaggt ccgagaggcc 1920
accaagatcg cccaggagct gcgacccgac ctggacatct acggccccat ccagtacgac 1980
gccgccgtcg acgagtccat cgccaagacc aagctgaagg ccatcccctc cggcgccaag 2040
gtcggcggcc aggccaacgt cctgatcttc cccgacctga acaccggcaa caacacctac 2100
aaggccgtcc agcagtccac cggctgcatc gccatgggcc ccatgctgca gggcctgcga 2160
aagcccgtca acgacctgtc ccgaggcgcc accgtcaagg acatcgtcac caccgtcgcc 2220
atcaccgcca tccaggccga ccaggtcatc atgaagcgag aggccgagaa cgccaccaag 2280
taa 2283
<210> 91
<211> 761
<212> PRT
<213> Phytophthora parasitica
<400> 91
Met Trp Thr Leu Arg Arg Ser Leu Arg His Ser Thr Gly Val Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Ala
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ala Asn Asp Ala Leu Ile Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Asp Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Ser Asn Val Asp Ser Leu Arg
210 215 220
Ala Ser Leu Lys Arg Lys Met Gly Asp Lys Asp Ile Pro Phe Leu Gly
225 230 235 240
Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu Val
245 250 255
Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly His Ser Ile Ala Asn
260 265 270
Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile Thr
290 295 300
Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg Leu
305 310 315 320
Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly Asn
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Gln Ala Leu
340 345 350
Asp Lys Thr Ala Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp Thr
355 360 365
Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile Leu Pro
370 375 380
Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Lys Ile Thr
450 455 460
Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn Ala Lys Met Ala Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Val Ser Pro Ala Asn Ser Ala Leu
485 490 495
Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys Arg Lys His Lys Gly
500 505 510
Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr Phe
515 520 525
Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys
565 570 575
Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu
660 665 670
Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn Val Leu
675 680 685
Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln
690 695 700
Gln Ser Thr Gly Cys Ile Ala Met Gly Pro Met Leu Gln Gly Leu Arg
705 710 715 720
Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp Ile Val
725 730 735
Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile Leu Lys
740 745 750
Arg Glu Ala Glu Thr Glu Ala Ala Ala
755 760
<210> 92
<211> 2286
<212> DNA
<213> Phytophthora parasitica
<400> 92
atgtggaccc tgcgacgatc cctgcgacac tccaccggcg tcgccctgcc ccaccgacga 60
gccctgaccg ccgccgccat ctcccagggc aaggtcccca tcaacaacct gtacgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctggccca cgtcctggag 180
cagaagttcg ccaaggtcgg ctacttccga cccatccagc cctcccccga ctcctccatg 240
gccgaccacc acgtcgacgt catgaagcag cagctggagc tgtccaagga cgtcgaggag 300
ctgtacggcg tcacctcctc ccgagccatg gaggccatgc tgaccggcaa gggcgacgac 360
gtcgtcgagg agatcctgga gcgatacgag cagtgccgaa agggccacga cttcatgatc 420
atcgagggct cccagatctc caagcacgag tccgccatgt cctggaagat caacgtcgac 480
atcgccaagg ccatcggctc ccccgtcctg atggtcaccg acttcggcga cacctccgcc 540
gccaacgacg ccctgatcga ggagatggtc tcccgaaccg tcatgggccg agaccaggcc 600
gaggacgccg gcctgaacta cctgggcacc atcgccaacc gagtccgagc ctccaacgtc 660
gactccctgc gagcctccct gaagcgaaag atgggcgaca aggacatccc cttcctgggc 720
ttcctgccca tggacgagat catcgcctcc aagcgactga acgaggtcac ccaccagctg 780
ggcgccaccc agctgttcgg ccactccatc gccaacgacg ccgtcgtcac ctccgccgtc 840
gtcgccgcct ccgccctgaa ggacctgttc gcccacctga agaagtacaa ggacggcgcc 900
atgatcatca cctccggcga ccgatccgac ctgatgctgg gcctgatggt ctcccgactg 960
cccggcgtcc tgcccaacat ctccgccatc gtcctgacca acggcaacta cccccactcc 1020
aacacccagg agatcctgaa gggcgtccag gccctggaca agaccgccct gtccctgccc 1080
atcttctcca cccccaacga caccttctcc accgccgacg gcttcgccaa ggtctccacc 1140
gacatcctgc cctcctccaa gctgaagatc gaccgatcca agcagctgtt cgacgagttc 1200
gtcgagaagg agatgctgat cggcgagctg gacgagggca tggtcgtcaa ccgatccccc 1260
aagcagttcc agcacttcct gttctccaag tcccgagccg tccagcgaca catcgtcctg 1320
accgagggcg aggacatccg agtcctgcag gccgccgacc agatcctgcg acagaacctg 1380
tccaagatca ccatcctggg cgaccccgac gagatcctgc tgaacgccaa gatggccaac 1440
ctggacctgt cccgagccaa catcgtctcc cccgccaact ccgccctgct ggacaagtac 1500
gtcgactact tctacgccaa gcgaaagcac aagggcgtca ccaaggagct ggcccgagac 1560
tactgcaagg acgagaccta cttcggcacc ctgatggtcg agctgggcga cgccgacggc 1620
atggtctccg gcgcctgcca caccaccgcc aacaccatcc gacccgccct gcagctgatc 1680
aagaccgccc ccaaccgacc catcgtctcc tccatcttct tcatgtgcct ggaggacggc 1740
gtccgaatct acggcgactg cgccgtcaac accgacccct ccgcccagga cctggcccag 1800
atcgccgtca cctccgccga gtccgccgag gccttcggcc tgatccccaa ggtcgccctg 1860
ctgtcctacg ccaccggcga ctccaactcc ggccccatca tcgacaaggt ccgagaggcc 1920
accaagatcg cccaggagct gcgacccgac ctggacatct acggccccat ccagtacgac 1980
gccgccgtcg acgagtccat cgccaagacc aagctgaagg ccatcccctc cggcgccaag 2040
gtcggcggcc aggccaacgt cctgatcttc cccgacctga acaccggcaa caacacctac 2100
aaggccgtcc agcagtccac cggctgcatc gccatgggcc ccatgctgca gggcctgcga 2160
aagcccgtca acgacctgtc ccgaggcgcc accgtcaagg acatcgtcac caccgtcgcc 2220
atcaccgcca tccaggccga ccaggtcatc ctgaagcgag aggccgagac cgaggccgcc 2280
gcctaa 2286
<210> 93
<211> 761
<212> PRT
<213> Phytophthora parasitica
<400> 93
Met Trp Thr Leu Arg Arg Ser Leu Arg His Ser Thr Gly Val Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Thr
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ala Asn Asp Ala Leu Ile Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Asp Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Ser Asn Val Asp Ser Leu Arg
210 215 220
Ala Ser Leu Lys Arg Lys Met Gly Asp Lys Asp Ile Pro Phe Leu Gly
225 230 235 240
Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu Val
245 250 255
Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly His Ser Ile Ala Asn
260 265 270
Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile Thr
290 295 300
Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg Leu
305 310 315 320
Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly Asn
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Gln Ala Leu
340 345 350
Asp Lys Thr Ala Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp Thr
355 360 365
Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile Leu Pro
370 375 380
Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Lys Ile Thr
450 455 460
Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn Ala Lys Met Ala Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Val Ser Pro Ala Asn Ser Ala Leu
485 490 495
Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys Arg Lys His Lys Gly
500 505 510
Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr Phe
515 520 525
Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys
565 570 575
Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu
660 665 670
Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn Val Leu
675 680 685
Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val Gln
690 695 700
Gln Ser Thr Gly Cys Ile Ala Met Gly Pro Met Leu Gln Gly Leu Arg
705 710 715 720
Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp Ile Val
725 730 735
Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile Leu Lys
740 745 750
Arg Glu Ala Glu Thr Glu Ala Ala Ala
755 760
<210> 94
<211> 2286
<212> DNA
<213> Phytophthora parasitica
<400> 94
atgtggaccc tgcgacgatc cctgcgacac tccaccggcg tcgccctgcc ccaccgacga 60
gccctgaccg ccgccgccat ctcccagggc aaggtcccca tcaacaacct gtacgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctggccca cgtcctggag 180
cagaagttca ccaaggtcgg ctacttccga cccatccagc cctcccccga ctcctccatg 240
gccgaccacc acgtcgacgt catgaagcag cagctggagc tgtccaagga cgtcgaggag 300
ctgtacggcg tcacctcctc ccgagccatg gaggccatgc tgaccggcaa gggcgacgac 360
gtcgtcgagg agatcctgga gcgatacgag cagtgccgaa agggccacga cttcatgatc 420
atcgagggct cccagatctc caagcacgag tccgccatgt cctggaagat caacgtcgac 480
atcgccaagg ccatcggctc ccccgtcctg atggtcaccg acttcggcga cacctccgcc 540
gccaacgacg ccctgatcga ggagatggtc tcccgaaccg tcatgggccg agaccaggcc 600
gaggacgccg gcctgaacta cctgggcacc atcgccaacc gagtccgagc ctccaacgtc 660
gactccctgc gagcctccct gaagcgaaag atgggcgaca aggacatccc cttcctgggc 720
ttcctgccca tggacgagat catcgcctcc aagcgactga acgaggtcac ccaccagctg 780
ggcgccaccc agctgttcgg ccactccatc gccaacgacg ccgtcgtcac ctccgccgtc 840
gtcgccgcct ccgccctgaa ggacctgttc gcccacctga agaagtacaa ggacggcgcc 900
atgatcatca cctccggcga ccgatccgac ctgatgctgg gcctgatggt ctcccgactg 960
cccggcgtcc tgcccaacat ctccgccatc gtcctgacca acggcaacta cccccactcc 1020
aacacccagg agatcctgaa gggcgtccag gccctggaca agaccgccct gtccctgccc 1080
atcttctcca cccccaacga caccttctcc accgccgacg gcttcgccaa ggtctccacc 1140
gacatcctgc cctcctccaa gctgaagatc gaccgatcca agcagctgtt cgacgagttc 1200
gtcgagaagg agatgctgat cggcgagctg gacgagggca tggtcgtcaa ccgatccccc 1260
aagcagttcc agcacttcct gttctccaag tcccgagccg tccagcgaca catcgtcctg 1320
accgagggcg aggacatccg agtcctgcag gccgccgacc agatcctgcg acagaacctg 1380
tccaagatca ccatcctggg cgaccccgac gagatcctgc tgaacgccaa gatggccaac 1440
ctggacctgt cccgagccaa catcgtctcc cccgccaact ccgccctgct ggacaagtac 1500
gtcgactact tctacgccaa gcgaaagcac aagggcgtca ccaaggagct ggcccgagac 1560
tactgcaagg acgagaccta cttcggcacc ctgatggtcg agctgggcga cgccgacggc 1620
atggtctccg gcgcctgcca caccaccgcc aacaccatcc gacccgccct gcagctgatc 1680
aagaccgccc ccaaccgacc catcgtctcc tccatcttct tcatgtgcct ggaggacggc 1740
gtccgaatct acggcgactg cgccgtcaac accgacccct ccgcccagga cctggcccag 1800
atcgccgtca cctccgccga gtccgccgag gccttcggcc tgatccccaa ggtcgccctg 1860
ctgtcctacg ccaccggcga ctccaactcc ggccccatca tcgacaaggt ccgagaggcc 1920
accaagatcg cccaggagct gcgacccgac ctggacatct acggccccat ccagtacgac 1980
gccgccgtcg acgagtccat cgccaagacc aagctgaagg ccatcccctc cggcgccaag 2040
gtcggcggcc aggccaacgt cctgatcttc cccgacctga acaccggcaa caacacctac 2100
aaggccgtcc agcagtccac cggctgcatc gccatgggcc ccatgctgca gggcctgcga 2160
aagcccgtca acgacctgtc ccgaggcgcc accgtcaagg acatcgtcac caccgtcgcc 2220
atcaccgcca tccaggccga ccaggtcatc ctgaagcgag aggccgagac cgaggccgcc 2280
gcctaa 2286
<210> 95
<211> 684
<212> PRT
<213> Phytophthora parasitica
<400> 95
Met Trp Thr Leu Arg Asn Thr Phe Arg Arg Thr Ser Ala Ala Phe Ala
1 5 10 15
Pro Gln Arg Arg Ala Leu Thr Ala Ala Ala Ile Ala Glu Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Phe Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Thr Asn Thr Leu Glu Gln Lys Phe Thr
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ile Val Glu Thr Asp His
65 70 75 80
His Ile Asp Val Met Lys Gln Gln Leu Gly Leu Thr Lys Ser Val Asp
85 90 95
Gln Leu Tyr Gly Val Thr Ser Glu Arg Ala Ile Glu Tyr Trp Leu Asn
100 105 110
Gly Lys Gly Asp Asp Leu Val Glu Glu Ile Leu Glu Arg Tyr Glu Ala
115 120 125
Cys Arg Glu Gly His Asp Phe Met Ile Ile Glu Gly Ser Gln Ile Ser
130 135 140
Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp Ile Ala Lys
145 150 155 160
Ala Ile Gly Ser Pro Val Leu Thr Ile Ser Asp Phe Ser Glu Ser Ala
165 170 175
Asn Thr Asn Gly Glu Leu Leu Glu Glu Ile Leu Ser Arg Thr Ala Leu
180 185 190
Asn Lys Asp Gln Val Glu Gly Ala Gly Leu Asn Phe Ile Gly Asn Ile
195 200 205
Ala Asn Arg Val Asn Thr Lys Asp Pro Lys Ala Leu Arg Asp Ala Ile
210 215 220
Arg Ser Lys Leu Arg Glu Lys Asp Leu Pro Phe Leu Gly Phe Leu Pro
225 230 235 240
Arg Asp Asp Phe Ile Ala Ser Lys Arg Leu Asn Glu Val Thr His Gln
245 250 255
Leu Gly Ala Lys Gln Leu Phe Gly Thr Lys Ala Ile Pro Asn Asn Val
260 265 270
Val Val Thr Ser Ala Val Val Ala Thr Ser Ala Leu Lys Asp Leu Phe
275 280 285
Ala His Leu Lys Asn Tyr Lys Asp Gly Ala Leu Val Ile Thr Ser Ala
290 295 300
Asp Arg Ser Asp Val Met Leu Gly Leu Met Ala Ser Arg Leu Pro Gly
305 310 315 320
Ile Leu Pro Asn Val Ser Ala Ile Val Leu Thr Asn Gly Ser Tyr Pro
325 330 335
His Ser Asn Thr Gln Glu Ile Leu Gln Gly Val Glu Ala Leu Asp Lys
340 345 350
Thr Gly Leu Ser Ile Pro Ile Phe Ser Val Pro Glu Asp Thr Phe Thr
355 360 365
Thr Ala Asp Lys Phe Ser Lys Val Ser Thr Asp Ile Leu Pro Thr Ser
370 375 380
Ser Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe Val Gly
385 390 395 400
Lys Glu Ser Ile Ile Gly Glu Leu Asp Glu Gly Met Val Val Asn Arg
405 410 415
Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg Ala Val
420 425 430
Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val Leu Gln
435 440 445
Ala Ala Asp Gln Val Leu Arg Gln Lys Leu Ser Lys Val Thr Ile Leu
450 455 460
Gly Asn Pro Asp Asp Ile Glu Arg His Ala Lys Ser Leu Asn Leu Asp
465 470 475 480
Leu Ser Arg Ala Asn Ile Val Arg Thr Ala Asp Ser Asp Leu Leu Glu
485 490 495
Arg Tyr Val Asp Gln Tyr Phe Glu Lys Arg Lys His Lys Gly Val Thr
500 505 510
Arg Glu Ser Ala Arg Asp Ala Val Leu Glu Glu Thr Cys Phe Gly Thr
515 520 525
Met Met Val Glu Met Gly Asp Ala Asp Gly Met Val Ser Gly Ala Cys
530 535 540
His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile Lys Thr
545 550 555 560
Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys Leu Glu
565 570 575
Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp Pro Ser
580 585 590
Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser Ala Glu
595 600 605
Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala Thr Gly
610 615 620
Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala Thr Lys
625 630 635 640
Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro Ile Gln
645 650 655
Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu Lys Ala
660 665 670
Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn
675 680
<210> 96
<211> 2055
<212> DNA
<213> Phytophthora parasitica
<400> 96
atgtggaccc tgcgaaacac cttccgacga acctccgccg ccttcgcccc ccagcgacga 60
gccctgaccg ccgccgccat cgccgagggc aaggtcccca tcaacaacct gttcgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctgaccaa caccctggag 180
cagaagttca ccaaggtcgg ctacttccga cccatccagc ccatcgtcga gaccgaccac 240
cacatcgacg tcatgaagca gcagctgggc ctgaccaagt ccgtcgacca gctgtacggc 300
gtcacctccg agcgagccat cgagtactgg ctgaacggca agggcgacga cctggtcgag 360
gagatcctgg agcgatacga ggcctgccga gagggccacg acttcatgat catcgagggc 420
tcccagatct ccaagcacga gtccgccatg tcctggaaga tcaacgtcga catcgccaag 480
gccatcggct cccccgtcct gaccatctcc gacttctccg agtccgccaa caccaacggc 540
gagctgctgg aggagatcct gtcccgaacc gccctgaaca aggaccaggt cgagggcgcc 600
ggcctgaact tcatcggcaa catcgccaac cgagtcaaca ccaaggaccc caaggccctg 660
cgagacgcca tccgatccaa gctgcgagag aaggacctgc ccttcctggg cttcctgccc 720
cgagacgact tcatcgcctc caagcgactg aacgaggtca cccaccagct gggcgccaag 780
cagctgttcg gcaccaaggc catccccaac aacgtcgtcg tcacctccgc cgtcgtcgcc 840
acctccgccc tgaaggacct gttcgcccac ctgaagaact acaaggacgg cgccctggtc 900
atcacctccg ccgaccgatc cgacgtcatg ctgggcctga tggcctcccg actgcccggc 960
atcctgccca acgtctccgc catcgtcctg accaacggct cctaccccca ctccaacacc 1020
caggagatcc tgcagggcgt cgaggccctg gacaagaccg gcctgtccat ccccatcttc 1080
tccgtccccg aggacacctt caccaccgcc gacaagttct ccaaggtctc caccgacatc 1140
ctgcccacct cctccctgaa gatcgaccga tccaagcagc tgttcgacga gttcgtcggc 1200
aaggagtcca tcatcggcga gctggacgag ggcatggtcg tcaaccgatc ccccaagcag 1260
ttccagcact tcctgttctc caagtcccga gccgtccagc gacacatcgt cctgaccgag 1320
ggcgaggaca tccgagtcct gcaggccgcc gaccaggtcc tgcgacagaa gctgtccaag 1380
gtcaccatcc tgggcaaccc cgacgacatc gagcgacacg ccaagtccct gaacctggac 1440
ctgtcccgag ccaacatcgt ccgaaccgcc gactccgacc tgctggagcg atacgtcgac 1500
cagtacttcg agaagcgaaa gcacaagggc gtcacccgag agtccgcccg agacgccgtc 1560
ctggaggaga cctgcttcgg caccatgatg gtcgagatgg gcgacgccga cggcatggtc 1620
tccggcgcct gccacaccac cgccaacacc atccgacccg ccctgcagct gatcaagacc 1680
gcccccaacc gacccatcgt ctcctccatc ttcttcatgt gcctggagga cggcgtccga 1740
atctacggcg actgcgccgt caacaccgac ccctccgccc aggacctggc ccagatcgcc 1800
gtcacctccg ccgagtccgc cgaggccttc ggcctgatcc ccaaggtcgc cctgctgtcc 1860
tacgccaccg gcgactccaa ctccggcccc atcatcgaca aggtccgaga ggccaccaag 1920
atcgcccagg agctgcgacc cgacctggac atctacggcc ccatccagta cgacgccgcc 1980
gtcgacgagt ccatcgccaa gaccaagctg aaggccatcc cctccggcgc caaggtcggc 2040
ggccaggcca actaa 2055
<210> 97
<211> 675
<212> PRT
<213> Phytophthora parasitica
<400> 97
Met Trp Thr Leu Arg Arg Ser Leu Arg His Ser Thr Gly Val Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Ala
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ala Asn Asp Ala Leu Ile Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Asp Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Ser Asn Val Asp Ser Leu His
210 215 220
Ile Pro Phe Leu Gly Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys
225 230 235 240
Arg Leu Asn Glu Val Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly
245 250 255
His Ser Ile Ala Asn Asp Ala Val Val Thr Ser Ala Val Val Ala Ala
260 265 270
Ser Ala Leu Lys Asp Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly
275 280 285
Ala Met Ile Ile Thr Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu
290 295 300
Met Val Ser Arg Leu Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val
305 310 315 320
Leu Thr Asn Gly Asn Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys
325 330 335
Gly Val Gln Ala Leu Asp Lys Thr Ala Leu Ser Leu Pro Ile Phe Ser
340 345 350
Thr Pro Asn Asp Thr Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser
355 360 365
Thr Asp Ile Leu Pro Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln
370 375 380
Leu Phe Asp Glu Phe Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp
385 390 395 400
Glu Gly Met Val Val Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu
405 410 415
Phe Ser Lys Ser Arg Ala Val Gln Arg His Ile Val Leu Thr Glu Gly
420 425 430
Glu Asp Ile Arg Val Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn
435 440 445
Leu Ser Lys Ile Thr Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn
450 455 460
Ala Lys Met Ala Asn Leu Asp Leu Ser Arg Ala Asn Ile Val Ser Pro
465 470 475 480
Ala Asn Ser Ala Leu Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys
485 490 495
Arg Lys His Lys Gly Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys
500 505 510
Asp Glu Thr Tyr Phe Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp
515 520 525
Gly Met Val Ser Gly Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro
530 535 540
Ala Leu Gln Leu Ile Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser
545 550 555 560
Ile Phe Phe Met Cys Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys
565 570 575
Ala Val Asn Thr Asp Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val
580 585 590
Thr Ser Ala Glu Ser Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala
595 600 605
Leu Leu Ser Tyr Ala Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp
610 615 620
Lys Val Arg Glu Ala Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu
625 630 635 640
Asp Ile Tyr Gly Pro Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile
645 650 655
Ala Lys Thr Lys Leu Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly
660 665 670
Gln Ala Asn
675
<210> 98
<211> 2028
<212> DNA
<213> Phytophthora parasitica
<400> 98
atgtggaccc tgcgacgatc cctgcgacac tccaccggcg tcgccctgcc ccaccgacga 60
gccctgaccg ccgccgccat ctcccagggc aaggtcccca tcaacaacct gtacgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctggccca cgtcctggag 180
cagaagttcg ccaaggtcgg ctacttccga cccatccagc cctcccccga ctcctccatg 240
gccgaccacc acgtcgacgt catgaagcag cagctggagc tgtccaagga cgtcgaggag 300
ctgtacggcg tcacctcctc ccgagccatg gaggccatgc tgaccggcaa gggcgacgac 360
gtcgtcgagg agatcctgga gcgatacgag cagtgccgaa agggccacga cttcatgatc 420
atcgagggct cccagatctc caagcacgag tccgccatgt cctggaagat caacgtcgac 480
atcgccaagg ccatcggctc ccccgtcctg atggtcaccg acttcggcga cacctccgcc 540
gccaacgacg ccctgatcga ggagatggtc tcccgaaccg tcatgggccg agaccaggcc 600
gaggacgccg gcctgaacta cctgggcacc atcgccaacc gagtccgagc ctccaacgtc 660
gactccctgc acatcccctt cctgggcttc ctgcccatgg acgagatcat cgcctccaag 720
cgactgaacg aggtcaccca ccagctgggc gccacccagc tgttcggcca ctccatcgcc 780
aacgacgccg tcgtcacctc cgccgtcgtc gccgcctccg ccctgaagga cctgttcgcc 840
cacctgaaga agtacaagga cggcgccatg atcatcacct ccggcgaccg atccgacctg 900
atgctgggcc tgatggtctc ccgactgccc ggcgtcctgc ccaacatctc cgccatcgtc 960
ctgaccaacg gcaactaccc ccactccaac acccaggaga tcctgaaggg cgtccaggcc 1020
ctggacaaga ccgccctgtc cctgcccatc ttctccaccc ccaacgacac cttctccacc 1080
gccgacggct tcgccaaggt ctccaccgac atcctgccct cctccaagct gaagatcgac 1140
cgatccaagc agctgttcga cgagttcgtc gagaaggaga tgctgatcgg cgagctggac 1200
gagggcatgg tcgtcaaccg atcccccaag cagttccagc acttcctgtt ctccaagtcc 1260
cgagccgtcc agcgacacat cgtcctgacc gagggcgagg acatccgagt cctgcaggcc 1320
gccgaccaga tcctgcgaca gaacctgtcc aagatcacca tcctgggcga ccccgacgag 1380
atcctgctga acgccaagat ggccaacctg gacctgtccc gagccaacat cgtctccccc 1440
gccaactccg ccctgctgga caagtacgtc gactacttct acgccaagcg aaagcacaag 1500
ggcgtcacca aggagctggc ccgagactac tgcaaggacg agacctactt cggcaccctg 1560
atggtcgagc tgggcgacgc cgacggcatg gtctccggcg cctgccacac caccgccaac 1620
accatccgac ccgccctgca gctgatcaag accgccccca accgacccat cgtctcctcc 1680
atcttcttca tgtgcctgga ggacggcgtc cgaatctacg gcgactgcgc cgtcaacacc 1740
gacccctccg cccaggacct ggcccagatc gccgtcacct ccgccgagtc cgccgaggcc 1800
ttcggcctga tccccaaggt cgccctgctg tcctacgcca ccggcgactc caactccggc 1860
cccatcatcg acaaggtccg agaggccacc aagatcgccc aggagctgcg acccgacctg 1920
gacatctacg gccccatcca gtacgacgcc gccgtcgacg agtccatcgc caagaccaag 1980
ctgaaggcca tcccctccgg cgccaaggtc ggcggccagg ccaactaa 2028
<210> 99
<211> 686
<212> PRT
<213> Phytophthora parasitica
<400> 99
Met Trp Thr Leu Arg Arg Ser Leu Arg His Ser Thr Gly Val Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Ala
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ala Asn Asp Ala Leu Ile Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Asp Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Ser Asn Val Asp Ser Leu Arg
210 215 220
Ala Ser Leu Lys Arg Lys Met Gly Asp Lys Asp Ile Pro Phe Leu Gly
225 230 235 240
Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu Val
245 250 255
Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly His Ser Ile Ala Asn
260 265 270
Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile Thr
290 295 300
Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg Leu
305 310 315 320
Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly Asn
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Gln Ala Leu
340 345 350
Asp Lys Thr Ala Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp Thr
355 360 365
Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile Leu Pro
370 375 380
Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Lys Ile Thr
450 455 460
Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn Ala Lys Met Ala Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Val Ser Pro Ala Asn Ser Ala Leu
485 490 495
Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys Arg Lys His Lys Gly
500 505 510
Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr Phe
515 520 525
Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys
565 570 575
Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu
660 665 670
Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn
675 680 685
<210> 100
<211> 2061
<212> DNA
<213> Phytophthora parasitica
<400> 100
atgtggaccc tgcgacgatc cctgcgacac tccaccggcg tcgccctgcc ccaccgacga 60
gccctgaccg ccgccgccat ctcccagggc aaggtcccca tcaacaacct gtacgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctggccca cgtcctggag 180
cagaagttcg ccaaggtcgg ctacttccga cccatccagc cctcccccga ctcctccatg 240
gccgaccacc acgtcgacgt catgaagcag cagctggagc tgtccaagga cgtcgaggag 300
ctgtacggcg tcacctcctc ccgagccatg gaggccatgc tgaccggcaa gggcgacgac 360
gtcgtcgagg agatcctgga gcgatacgag cagtgccgaa agggccacga cttcatgatc 420
atcgagggct cccagatctc caagcacgag tccgccatgt cctggaagat caacgtcgac 480
atcgccaagg ccatcggctc ccccgtcctg atggtcaccg acttcggcga cacctccgcc 540
gccaacgacg ccctgatcga ggagatggtc tcccgaaccg tcatgggccg agaccaggcc 600
gaggacgccg gcctgaacta cctgggcacc atcgccaacc gagtccgagc ctccaacgtc 660
gactccctgc gagcctccct gaagcgaaag atgggcgaca aggacatccc cttcctgggc 720
ttcctgccca tggacgagat catcgcctcc aagcgactga acgaggtcac ccaccagctg 780
ggcgccaccc agctgttcgg ccactccatc gccaacgacg ccgtcgtcac ctccgccgtc 840
gtcgccgcct ccgccctgaa ggacctgttc gcccacctga agaagtacaa ggacggcgcc 900
atgatcatca cctccggcga ccgatccgac ctgatgctgg gcctgatggt ctcccgactg 960
cccggcgtcc tgcccaacat ctccgccatc gtcctgacca acggcaacta cccccactcc 1020
aacacccagg agatcctgaa gggcgtccag gccctggaca agaccgccct gtccctgccc 1080
atcttctcca cccccaacga caccttctcc accgccgacg gcttcgccaa ggtctccacc 1140
gacatcctgc cctcctccaa gctgaagatc gaccgatcca agcagctgtt cgacgagttc 1200
gtcgagaagg agatgctgat cggcgagctg gacgagggca tggtcgtcaa ccgatccccc 1260
aagcagttcc agcacttcct gttctccaag tcccgagccg tccagcgaca catcgtcctg 1320
accgagggcg aggacatccg agtcctgcag gccgccgacc agatcctgcg acagaacctg 1380
tccaagatca ccatcctggg cgaccccgac gagatcctgc tgaacgccaa gatggccaac 1440
ctggacctgt cccgagccaa catcgtctcc cccgccaact ccgccctgct ggacaagtac 1500
gtcgactact tctacgccaa gcgaaagcac aagggcgtca ccaaggagct ggcccgagac 1560
tactgcaagg acgagaccta cttcggcacc ctgatggtcg agctgggcga cgccgacggc 1620
atggtctccg gcgcctgcca caccaccgcc aacaccatcc gacccgccct gcagctgatc 1680
aagaccgccc ccaaccgacc catcgtctcc tccatcttct tcatgtgcct ggaggacggc 1740
gtccgaatct acggcgactg cgccgtcaac accgacccct ccgcccagga cctggcccag 1800
atcgccgtca cctccgccga gtccgccgag gccttcggcc tgatccccaa ggtcgccctg 1860
ctgtcctacg ccaccggcga ctccaactcc ggccccatca tcgacaaggt ccgagaggcc 1920
accaagatcg cccaggagct gcgacccgac ctggacatct acggccccat ccagtacgac 1980
gccgccgtcg acgagtccat cgccaagacc aagctgaagg ccatcccctc cggcgccaag 2040
gtcggcggcc aggccaacta a 2061
<210> 101
<211> 676
<212> PRT
<213> Phytophthora parasitica
<400> 101
Met Trp Thr Leu Arg Arg Ser Leu Arg His Ser Thr Gly Val Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Ala
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ala Asn Asp Ala Leu Ile Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Asp Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Ser Asn Val Asp Ser Leu Arg
210 215 220
Ala Ser Leu Lys Arg Lys Met Gly Asp Lys Asp Ile Pro Phe Leu Gly
225 230 235 240
Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu Val
245 250 255
Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly His Ser Ile Ala Asn
260 265 270
Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile Thr
290 295 300
Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg Leu
305 310 315 320
Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly Asn
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Gln Ala Leu
340 345 350
Asp Lys Thr Ala Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp Thr
355 360 365
Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile Leu Pro
370 375 380
Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Gln Ile Thr
450 455 460
Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn Ala Lys Met Ala Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Val Ser Pro Ala Asn Ser Ala Leu
485 490 495
Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys Arg Lys His Lys Gly
500 505 510
Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr Phe
515 520 525
Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys
565 570 575
Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile Gln Tyr Asp Ala Ala Val Asp Glu Ser Ile Ala Lys Thr Lys Leu
660 665 670
Lys Ala Ile Pro
675
<210> 102
<211> 2031
<212> DNA
<213> Phytophthora parasitica
<400> 102
atgtggaccc tgcgacgatc cctgcgacac tccaccggcg tcgccctgcc ccaccgacga 60
gccctgaccg ccgccgccat ctcccagggc aaggtcccca tcaacaacct gtacgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctggccca cgtcctggag 180
cagaagttcg ccaaggtcgg ctacttccga cccatccagc cctcccccga ctcctccatg 240
gccgaccacc acgtcgacgt catgaagcag cagctggagc tgtccaagga cgtcgaggag 300
ctgtacggcg tcacctcctc ccgagccatg gaggccatgc tgaccggcaa gggcgacgac 360
gtcgtcgagg agatcctgga gcgatacgag cagtgccgaa agggccacga cttcatgatc 420
atcgagggct cccagatctc caagcacgag tccgccatgt cctggaagat caacgtcgac 480
atcgccaagg ccatcggctc ccccgtcctg atggtcaccg acttcggcga cacctccgcc 540
gccaacgacg ccctgatcga ggagatggtc tcccgaaccg tcatgggccg agaccaggcc 600
gaggacgccg gcctgaacta cctgggcacc atcgccaacc gagtccgagc ctccaacgtc 660
gactccctgc gagcctccct gaagcgaaag atgggcgaca aggacatccc cttcctgggc 720
ttcctgccca tggacgagat catcgcctcc aagcgactga acgaggtcac ccaccagctg 780
ggcgccaccc agctgttcgg ccactccatc gccaacgacg ccgtcgtcac ctccgccgtc 840
gtcgccgcct ccgccctgaa ggacctgttc gcccacctga agaagtacaa ggacggcgcc 900
atgatcatca cctccggcga ccgatccgac ctgatgctgg gcctgatggt ctcccgactg 960
cccggcgtcc tgcccaacat ctccgccatc gtcctgacca acggcaacta cccccactcc 1020
aacacccagg agatcctgaa gggcgtccag gccctggaca agaccgccct gtccctgccc 1080
atcttctcca cccccaacga caccttctcc accgccgacg gcttcgccaa ggtctccacc 1140
gacatcctgc cctcctccaa gctgaagatc gaccgatcca agcagctgtt cgacgagttc 1200
gtcgagaagg agatgctgat cggcgagctg gacgagggca tggtcgtcaa ccgatccccc 1260
aagcagttcc agcacttcct gttctccaag tcccgagccg tccagcgaca catcgtcctg 1320
accgagggcg aggacatccg agtcctgcag gccgccgacc agatcctgcg acagaacctg 1380
tcccagatca ccatcctggg cgaccccgac gagatcctgc tgaacgccaa gatggccaac 1440
ctggacctgt cccgagccaa catcgtctcc cccgccaact ccgccctgct ggacaagtac 1500
gtcgactact tctacgccaa gcgaaagcac aagggcgtca ccaaggagct ggcccgagac 1560
tactgcaagg acgagaccta cttcggcacc ctgatggtcg agctgggcga cgccgacggc 1620
atggtctccg gcgcctgcca caccaccgcc aacaccatcc gacccgccct gcagctgatc 1680
aagaccgccc ccaaccgacc catcgtctcc tccatcttct tcatgtgcct ggaggacggc 1740
gtccgaatct acggcgactg cgccgtcaac accgacccct ccgcccagga cctggcccag 1800
atcgccgtca cctccgccga gtccgccgag gccttcggcc tgatccccaa ggtcgccctg 1860
ctgtcctacg ccaccggcga ctccaactcc ggccccatca tcgacaaggt ccgagaggcc 1920
accaagatcg cccaggagct gcgacccgac ctggacatct acggccccat ccagtacgac 1980
gccgccgtcg acgagtccat cgccaagacc aagctgaagg ccatccccta a 2031
<210> 103
<211> 655
<212> PRT
<213> Phytophthora parasitica
<400> 103
Met Trp Thr Leu Arg Asn Thr Phe Arg Arg Thr Ser Ala Ala Phe Ala
1 5 10 15
Pro Gln Arg Arg Ala Leu Thr Ala Ala Ala Ile Ala Glu Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Phe Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Thr Asn Thr Leu Glu Gln Lys Phe Thr
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ile Val Glu Thr Asp His
65 70 75 80
His Ile Asp Val Met Lys Gln Gln Leu Gly Leu Thr Lys Ser Val Asp
85 90 95
Gln Leu Tyr Gly Val Thr Ser Glu Arg Ala Ile Glu Tyr Trp Leu Asn
100 105 110
Gly Lys Gly Asp Asp Leu Val Glu Glu Ile Leu Glu Arg Tyr Glu Ala
115 120 125
Cys Arg Glu Gly His Asp Phe Met Ile Ile Glu Gly Ser Gln Ile Ser
130 135 140
Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp Ile Ala Lys
145 150 155 160
Ala Ile Gly Ser Pro Val Leu Thr Ile Ser Asp Phe Ser Glu Ser Ala
165 170 175
Asn Thr Asn Gly Glu Leu Leu Glu Glu Ile Leu Ser Arg Thr Ala Leu
180 185 190
Asn Lys Asp Gln Val Glu Gly Ala Gly Leu Asn Phe Ile Gly Asn Ile
195 200 205
Ala Asn Arg Val Asn Thr Lys Asp Pro Lys Ala Leu Arg Asp Ala Ile
210 215 220
Arg Ser Lys Leu Arg Glu Lys Asp Leu Pro Phe Leu Gly Phe Leu Pro
225 230 235 240
Arg Asp Asp Phe Ile Ala Ser Lys Arg Leu Asn Glu Val Thr His Gln
245 250 255
Leu Gly Ala Lys Gln Leu Phe Gly Thr Lys Ala Ile Pro Asn Asn Val
260 265 270
Val Val Thr Ser Ala Val Val Ala Thr Ser Ala Leu Lys Asp Leu Phe
275 280 285
Ala His Leu Lys Asn Tyr Lys Asp Gly Ala Leu Val Ile Thr Ser Ala
290 295 300
Asp Arg Ser Asp Val Met Leu Gly Leu Met Ala Ser Arg Leu Pro Gly
305 310 315 320
Ile Leu Pro Asn Val Ser Ala Ile Val Leu Thr Asn Gly Ser Tyr Pro
325 330 335
His Ser Asn Thr Gln Glu Ile Leu Gln Gly Val Glu Ala Leu Asp Lys
340 345 350
Thr Gly Leu Ser Ile Pro Ile Phe Ser Val Pro Glu Asp Thr Phe Thr
355 360 365
Thr Ala Asp Lys Phe Ser Lys Val Ser Thr Asp Ile Leu Pro Thr Ser
370 375 380
Ser Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe Val Gly
385 390 395 400
Lys Glu Ser Ile Ile Gly Glu Leu Asp Glu Gly Met Val Val Asn Arg
405 410 415
Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg Ala Val
420 425 430
Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val Leu Gln
435 440 445
Ala Ala Asp Gln Val Leu Arg Gln Lys Leu Ser Lys Val Thr Ile Leu
450 455 460
Gly Asn Pro Asp Asp Ile Glu Arg His Ala Lys Ser Leu Asn Leu Asp
465 470 475 480
Leu Ser Arg Ala Asn Ile Val Arg Thr Ala Asp Ser Asp Leu Leu Glu
485 490 495
Arg Tyr Val Asp Gln Tyr Phe Glu Lys Arg Lys His Lys Gly Val Thr
500 505 510
Arg Glu Ser Ala Arg Asp Ala Val Leu Glu Glu Thr Cys Phe Gly Thr
515 520 525
Met Met Val Glu Met Gly Asp Ala Asp Gly Met Val Ser Gly Ala Cys
530 535 540
His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile Lys Thr
545 550 555 560
Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys Leu Glu
565 570 575
Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp Pro Ser
580 585 590
Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser Ala Glu
595 600 605
Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala Thr Gly
610 615 620
Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala Thr Lys
625 630 635 640
Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro Ile
645 650 655
<210> 104
<211> 1968
<212> DNA
<213> Phytophthora parasitica
<400> 104
atgtggaccc tgcgaaacac cttccgacga acctccgccg ccttcgcccc ccagcgacga 60
gccctgaccg ccgccgccat cgccgagggc aaggtcccca tcaacaacct gttcgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctgaccaa caccctggag 180
cagaagttca ccaaggtcgg ctacttccga cccatccagc ccatcgtcga gaccgaccac 240
cacatcgacg tcatgaagca gcagctgggc ctgaccaagt ccgtcgacca gctgtacggc 300
gtcacctccg agcgagccat cgagtactgg ctgaacggca agggcgacga cctggtcgag 360
gagatcctgg agcgatacga ggcctgccga gagggccacg acttcatgat catcgagggc 420
tcccagatct ccaagcacga gtccgccatg tcctggaaga tcaacgtcga catcgccaag 480
gccatcggct cccccgtcct gaccatctcc gacttctccg agtccgccaa caccaacggc 540
gagctgctgg aggagatcct gtcccgaacc gccctgaaca aggaccaggt cgagggcgcc 600
ggcctgaact tcatcggcaa catcgccaac cgagtcaaca ccaaggaccc caaggccctg 660
cgagacgcca tccgatccaa gctgcgagag aaggacctgc ccttcctggg cttcctgccc 720
cgagacgact tcatcgcctc caagcgactg aacgaggtca cccaccagct gggcgccaag 780
cagctgttcg gcaccaaggc catccccaac aacgtcgtcg tcacctccgc cgtcgtcgcc 840
acctccgccc tgaaggacct gttcgcccac ctgaagaact acaaggacgg cgccctggtc 900
atcacctccg ccgaccgatc cgacgtcatg ctgggcctga tggcctcccg actgcccggc 960
atcctgccca acgtctccgc catcgtcctg accaacggct cctaccccca ctccaacacc 1020
caggagatcc tgcagggcgt cgaggccctg gacaagaccg gcctgtccat ccccatcttc 1080
tccgtccccg aggacacctt caccaccgcc gacaagttct ccaaggtctc caccgacatc 1140
ctgcccacct cctccctgaa gatcgaccga tccaagcagc tgttcgacga gttcgtcggc 1200
aaggagtcca tcatcggcga gctggacgag ggcatggtcg tcaaccgatc ccccaagcag 1260
ttccagcact tcctgttctc caagtcccga gccgtccagc gacacatcgt cctgaccgag 1320
ggcgaggaca tccgagtcct gcaggccgcc gaccaggtcc tgcgacagaa gctgtccaag 1380
gtcaccatcc tgggcaaccc cgacgacatc gagcgacacg ccaagtccct gaacctggac 1440
ctgtcccgag ccaacatcgt ccgaaccgcc gactccgacc tgctggagcg atacgtcgac 1500
cagtacttcg agaagcgaaa gcacaagggc gtcacccgag agtccgcccg agacgccgtc 1560
ctggaggaga cctgcttcgg caccatgatg gtcgagatgg gcgacgccga cggcatggtc 1620
tccggcgcct gccacaccac cgccaacacc atccgacccg ccctgcagct gatcaagacc 1680
gcccccaacc gacccatcgt ctcctccatc ttcttcatgt gcctggagga cggcgtccga 1740
atctacggcg actgcgccgt caacaccgac ccctccgccc aggacctggc ccagatcgcc 1800
gtcacctccg ccgagtccgc cgaggccttc ggcctgatcc ccaaggtcgc cctgctgtcc 1860
tacgccaccg gcgactccaa ctccggcccc atcatcgaca aggtccgaga ggccaccaag 1920
atcgcccagg agctgcgacc cgacctggac atctacggcc ccatctaa 1968
<210> 105
<211> 657
<212> PRT
<213> Phytophthora parasitica
<400> 105
Met Trp Thr Leu Arg Arg Ser Leu Arg His Ser Thr Gly Val Ala Leu
1 5 10 15
Pro His Arg Arg Ala Leu Thr Ala Ala Ala Ile Ser Gln Gly Lys Val
20 25 30
Pro Ile Asn Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys Thr
35 40 45
Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe Thr
50 55 60
Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Pro Asp Ser Ser Met
65 70 75 80
Ala Asp His His Val Asp Val Met Lys Gln Gln Leu Glu Leu Ser Lys
85 90 95
Asp Val Glu Glu Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu Ala
100 105 110
Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Glu Arg
115 120 125
Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly Ser
130 135 140
Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val Asp
145 150 155 160
Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe Gly
165 170 175
Asp Thr Ser Ala Ala Asn Asp Ala Leu Ile Glu Glu Met Val Ser Arg
180 185 190
Thr Val Met Gly Arg Asp Gln Ala Glu Asp Ala Gly Leu Asn Tyr Leu
195 200 205
Gly Thr Ile Ala Asn Arg Val Arg Ala Ser Asn Val Asp Ser Leu Arg
210 215 220
Ala Ser Leu Lys Arg Lys Met Gly Asp Lys Asp Ile Pro Phe Leu Gly
225 230 235 240
Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu Val
245 250 255
Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly His Ser Ile Ala Asn
260 265 270
Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys Asp
275 280 285
Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile Thr
290 295 300
Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg Leu
305 310 315 320
Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly Asn
325 330 335
Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Gln Ala Leu
340 345 350
Asp Lys Thr Ala Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp Thr
355 360 365
Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile Leu Pro
370 375 380
Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu Phe
385 390 395 400
Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Glu Gly Met Val Val
405 410 415
Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser Arg
420 425 430
Ala Val Gln Arg His Ile Val Leu Thr Glu Gly Glu Asp Ile Arg Val
435 440 445
Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Lys Ile Thr
450 455 460
Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn Ala Lys Met Ala Asn
465 470 475 480
Leu Asp Leu Ser Arg Ala Asn Ile Val Ser Pro Ala Asn Ser Ala Leu
485 490 495
Leu Asp Lys Tyr Val Asp Tyr Phe Tyr Ala Lys Arg Lys His Lys Gly
500 505 510
Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr Phe
515 520 525
Gly Thr Leu Met Val Glu Leu Gly Asp Ala Asp Gly Met Val Ser Gly
530 535 540
Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu Ile
545 550 555 560
Lys Thr Ala Pro Asn Arg Pro Ile Val Ser Ser Ile Phe Phe Met Cys
565 570 575
Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr Asp
580 585 590
Pro Ser Ala Gln Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu Ser
595 600 605
Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr Ala
610 615 620
Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu Ala
625 630 635 640
Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly Pro
645 650 655
Ile
<210> 106
<211> 1974
<212> DNA
<213> Phytophthora parasitica
<400> 106
atgtggaccc tgcgacgatc cctgcgacac tccaccggcg tcgccctgcc ccaccgacga 60
gccctgaccg ccgccgccat ctcccagggc aaggtcccca tcaacaacct gtacgtcacc 120
tccaccgagg tcaccaagaa gaccgccccc gtcctgatcg gcctggccca cgtcctggag 180
cagaagttca ccaaggtcgg ctacttccga cccatccagc cctcccccga ctcctccatg 240
gccgaccacc acgtcgacgt catgaagcag cagctggagc tgtccaagga cgtcgaggag 300
ctgtacggcg tcacctcctc ccgagccatg gaggccatgc tgaccggcaa gggcgacgac 360
gtcgtcgagg agatcctgga gcgatacgag cagtgccgaa agggccacga cttcatgatc 420
atcgagggct cccagatctc caagcacgag tccgccatgt cctggaagat caacgtcgac 480
atcgccaagg ccatcggctc ccccgtcctg atggtcaccg acttcggcga cacctccgcc 540
gccaacgacg ccctgatcga ggagatggtc tcccgaaccg tcatgggccg agaccaggcc 600
gaggacgccg gcctgaacta cctgggcacc atcgccaacc gagtccgagc ctccaacgtc 660
gactccctgc gagcctccct gaagcgaaag atgggcgaca aggacatccc cttcctgggc 720
ttcctgccca tggacgagat catcgcctcc aagcgactga acgaggtcac ccaccagctg 780
ggcgccaccc agctgttcgg ccactccatc gccaacgacg ccgtcgtcac ctccgccgtc 840
gtcgccgcct ccgccctgaa ggacctgttc gcccacctga agaagtacaa ggacggcgcc 900
atgatcatca cctccggcga ccgatccgac ctgatgctgg gcctgatggt ctcccgactg 960
cccggcgtcc tgcccaacat ctccgccatc gtcctgacca acggcaacta cccccactcc 1020
aacacccagg agatcctgaa gggcgtccag gccctggaca agaccgccct gtccctgccc 1080
atcttctcca cccccaacga caccttctcc accgccgacg gcttcgccaa ggtctccacc 1140
gacatcctgc cctcctccaa gctgaagatc gaccgatcca agcagctgtt cgacgagttc 1200
gtcgagaagg agatgctgat cggcgagctg gacgagggca tggtcgtcaa ccgatccccc 1260
aagcagttcc agcacttcct gttctccaag tcccgagccg tccagcgaca catcgtcctg 1320
accgagggcg aggacatccg agtcctgcag gccgccgacc agatcctgcg acagaacctg 1380
tccaagatca ccatcctggg cgaccccgac gagatcctgc tgaacgccaa gatggccaac 1440
ctggacctgt cccgagccaa catcgtctcc cccgccaact ccgccctgct ggacaagtac 1500
gtcgactact tctacgccaa gcgaaagcac aagggcgtca ccaaggagct ggcccgagac 1560
tactgcaagg acgagaccta cttcggcacc ctgatggtcg agctgggcga cgccgacggc 1620
atggtctccg gcgcctgcca caccaccgcc aacaccatcc gacccgccct gcagctgatc 1680
aagaccgccc ccaaccgacc catcgtctcc tccatcttct tcatgtgcct ggaggacggc 1740
gtccgaatct acggcgactg cgccgtcaac accgacccct ccgcccagga cctggcccag 1800
atcgccgtca cctccgccga gtccgccgag gccttcggcc tgatccccaa ggtcgccctg 1860
ctgtcctacg ccaccggcga ctccaactcc ggccccatca tcgacaaggt ccgagaggcc 1920
accaagatcg cccaggagct gcgacccgac ctggacatct acggccccat ctaa 1974
<210> 107
<211> 760
<212> PRT
<213> Phytophthora ramorum
<400> 107
Met Trp Thr Leu Arg Arg Ser Leu Arg Arg Ser Thr Ala Ser Ala Thr
1 5 10 15
Leu Pro Thr Arg Arg Ala Leu Thr Ala Ala Ser Ile Ala Gln Gly Lys
20 25 30
Val Pro Ile Glu Asn Leu Tyr Val Thr Ser Thr Glu Val Thr Lys Lys
35 40 45
Thr Ala Pro Val Leu Ile Gly Leu Ala His Val Leu Glu Gln Lys Phe
50 55 60
Ser Lys Val Gly Tyr Phe Arg Pro Ile Gln Pro Ser Arg Asp Ser Ser
65 70 75 80
Met Ala Asp His His Val Asp Val Met Arg Gln Gln Leu Glu Leu Ser
85 90 95
Gln Asp Val Glu Gln Leu Tyr Gly Val Thr Ser Ser Arg Ala Met Glu
100 105 110
Ala Met Leu Thr Gly Lys Gly Asp Asp Val Val Glu Glu Ile Leu Asp
115 120 125
Arg Tyr Glu Gln Cys Arg Lys Gly His Asp Phe Met Ile Ile Glu Gly
130 135 140
Ser Gln Ile Ser Lys His Glu Ser Ala Met Ser Trp Lys Ile Asn Val
145 150 155 160
Asp Ile Ala Lys Ala Ile Gly Ser Pro Val Leu Met Val Thr Asp Phe
165 170 175
Gly Asp Ser Ser Ala Val Asn Gly Glu Leu Leu Glu Glu Met Val Ser
180 185 190
Arg Thr Leu Met Gly Arg Asp Gln Ala Asp Ala Ala Gly Leu Asn Tyr
195 200 205
Leu Gly Thr Ile Ala Asn Arg Val Arg Ala Lys Asp Ala Glu Ala Leu
210 215 220
Arg Ala Glu Leu Lys Lys Thr Val Ser Gly Lys Glu Ile Pro Phe Leu
225 230 235 240
Gly Phe Leu Pro Met Asp Glu Ile Ile Ala Ser Lys Arg Leu Asn Glu
245 250 255
Val Thr His Gln Leu Gly Ala Thr Gln Leu Phe Gly Asn Ser Ile Ala
260 265 270
Asn Asp Ala Val Val Thr Ser Ala Val Val Ala Ala Ser Ala Leu Lys
275 280 285
Asp Leu Phe Ala His Leu Lys Lys Tyr Lys Asp Gly Ala Met Ile Ile
290 295 300
Thr Ser Gly Asp Arg Ser Asp Leu Met Leu Gly Leu Met Val Ser Arg
305 310 315 320
Leu Pro Gly Val Leu Pro Asn Ile Ser Ala Ile Val Leu Thr Asn Gly
325 330 335
Asn Tyr Pro His Ser Asn Thr Gln Glu Ile Leu Lys Gly Val Glu Ala
340 345 350
Leu Asp Lys Thr Gly Leu Ser Leu Pro Ile Phe Ser Thr Pro Asn Asp
355 360 365
Thr Phe Ser Thr Ala Asp Gly Phe Ala Lys Val Ser Thr Asp Ile Leu
370 375 380
Pro Ser Ser Lys Leu Lys Ile Asp Arg Ser Lys Gln Leu Phe Asp Glu
385 390 395 400
Phe Val Glu Lys Glu Met Leu Ile Gly Glu Leu Asp Gln Gly Met Val
405 410 415
Val Asn Arg Ser Pro Lys Gln Phe Gln His Phe Leu Phe Ser Lys Ser
420 425 430
Arg Ala Val Gln Arg Arg Ile Val Leu Thr Glu Gly Glu Asp Ile Arg
435 440 445
Val Leu Gln Ala Ala Asp Gln Ile Leu Arg Gln Asn Leu Ser Lys Ile
450 455 460
Thr Ile Leu Gly Asp Pro Asp Glu Ile Leu Leu Asn Ala Lys Thr Ser
465 470 475 480
Asn Leu Asp Leu Ser Arg Ala Asn Ile Val Arg Pro Ala Asp Ser Asp
485 490 495
Leu Leu Glu Lys Tyr Val Asp Tyr Phe Tyr Glu Lys Arg Lys His Lys
500 505 510
Gly Val Thr Lys Glu Leu Ala Arg Asp Tyr Cys Lys Asp Glu Thr Tyr
515 520 525
Phe Gly Thr Leu Met Val Glu Met Gly Asp Ala Asp Gly Met Val Ser
530 535 540
Gly Ala Cys His Thr Thr Ala Asn Thr Ile Arg Pro Ala Leu Gln Leu
545 550 555 560
Ile Lys Thr Thr Pro Asn Arg Pro Ile Val Ser Ser Val Phe Phe Met
565 570 575
Cys Leu Glu Asp Gly Val Arg Ile Tyr Gly Asp Cys Ala Val Asn Thr
580 585 590
Asp Pro Ser Ala Ala Asp Leu Ala Gln Ile Ala Val Thr Ser Ala Glu
595 600 605
Ser Ala Glu Ala Phe Gly Leu Ile Pro Lys Val Ala Leu Leu Ser Tyr
610 615 620
Ala Thr Gly Asp Ser Asn Ser Gly Pro Ile Ile Asp Lys Val Arg Glu
625 630 635 640
Ala Thr Lys Ile Ala Gln Glu Leu Arg Pro Asp Leu Asp Ile Tyr Gly
645 650 655
Pro Ile Gln Tyr Asp Ala Ala Val Asp Ala Ser Ile Ala Lys Thr Lys
660 665 670
Leu Lys Ala Ile Pro Ser Gly Ala Lys Val Gly Gly Gln Ala Asn Val
675 680 685
Leu Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr Lys Ala Val
690 695 700
Gln Gln Ser Thr Gly Cys Val Ala Met Gly Pro Met Leu Gln Gly Leu
705 710 715 720
Arg Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val Lys Asp Ile
725 730 735
Val Thr Thr Val Ala Ile Thr Ala Ile Gln Ala Asp Gln Val Ile Leu
740 745 750
Lys Arg Glu Ala Ala Thr Lys Lys
755 760
<210> 108
<211> 2283
<212> DNA
<213> Phytophthora ramorum
<400> 108
atgtggaccc tgcgacgatc cctgcgacga tccaccgcct ccgccaccct gcccacccga 60
cgagccctga ccgccgcctc catcgcccag ggcaaggtcc ccatcgagaa cctgtacgtc 120
acctccaccg aggtcaccaa gaagaccgcc cccgtcctga tcggcctggc ccacgtcctg 180
gagcagaagt tctccaaggt cggctacttc cgacccatcc agccctcccg agactcctcc 240
atggccgacc accacgtcga cgtcatgcga cagcagctgg agctgtccca ggacgtcgag 300
cagctgtacg gcgtcacctc ctcccgagcc atggaggcca tgctgaccgg caagggcgac 360
gacgtcgtcg aggagatcct ggaccgatac gagcagtgcc gaaagggcca cgacttcatg 420
atcatcgagg gctcccagat ctccaagcac gagtccgcca tgtcctggaa gatcaacgtc 480
gacatcgcca aggccatcgg ctcccccgtc ctgatggtca ccgacttcgg cgactcctcc 540
gccgtcaacg gcgagctgct ggaggagatg gtctcccgaa ccctgatggg ccgagaccag 600
gccgacgccg ccggcctgaa ctacctgggc accatcgcca accgagtccg agccaaggac 660
gccgaggccc tgcgagccga gctgaagaag accgtctccg gcaaggagat ccccttcctg 720
ggcttcctgc ccatggacga gatcatcgcc tccaagcgac tgaacgaggt cacccaccag 780
ctgggcgcca cccagctgtt cggcaactcc atcgccaacg acgccgtcgt cacctccgcc 840
gtcgtcgccg cctccgccct gaaggacctg ttcgcccacc tgaagaagta caaggacggc 900
gccatgatca tcacctccgg cgaccgatcc gacctgatgc tgggcctgat ggtctcccga 960
ctgcccggcg tcctgcccaa catctccgcc atcgtcctga ccaacggcaa ctacccccac 1020
tccaacaccc aggagatcct gaagggcgtc gaggccctgg acaagaccgg cctgtccctg 1080
cccatcttct ccacccccaa cgacaccttc tccaccgccg acggcttcgc caaggtctcc 1140
accgacatcc tgccctcctc caagctgaag atcgaccgat ccaagcagct gttcgacgag 1200
ttcgtcgaga aggagatgct gatcggcgag ctggaccagg gcatggtcgt caaccgatcc 1260
cccaagcagt tccagcactt cctgttctcc aagtcccgag ccgtccagcg acgaatcgtc 1320
ctgaccgagg gcgaggacat ccgagtcctg caggccgccg accagatcct gcgacagaac 1380
ctgtccaaga tcaccatcct gggcgacccc gacgagatcc tgctgaacgc caagacctcc 1440
aacctggacc tgtcccgagc caacatcgtc cgacccgccg actccgacct gctggagaag 1500
tacgtcgact acttctacga gaagcgaaag cacaagggcg tcaccaagga gctggcccga 1560
gactactgca aggacgagac ctacttcggc accctgatgg tcgagatggg cgacgccgac 1620
ggcatggtct ccggcgcctg ccacaccacc gccaacacca tccgacccgc cctgcagctg 1680
atcaagacca cccccaaccg acccatcgtc tcctccgtct tcttcatgtg cctggaggac 1740
ggcgtccgaa tctacggcga ctgcgccgtc aacaccgacc cctccgccgc cgacctggcc 1800
cagatcgccg tcacctccgc cgagtccgcc gaggccttcg gcctgatccc caaggtcgcc 1860
ctgctgtcct acgccaccgg cgactccaac tccggcccca tcatcgacaa ggtccgagag 1920
gccaccaaga tcgcccagga gctgcgaccc gacctggaca tctacggccc catccagtac 1980
gacgccgccg tcgacgcctc catcgccaag accaagctga aggccatccc ctccggcgcc 2040
aaggtcggcg gccaggccaa cgtcctgatc ttccccgacc tgaacaccgg caacaacacc 2100
tacaaggccg tccagcagtc caccggctgc gtcgccatgg gccccatgct gcagggcctg 2160
cgaaagcccg tcaacgacct gtcccgaggc gccaccgtca aggacatcgt caccaccgtc 2220
gccatcaccg ccatccaggc cgaccaggtc atcctgaagc gagaggccgc caccaagaag 2280
taa 2283
<210> 109
<211> 743
<212> PRT
<213> Saprolegnia parasitica
<400> 109
Met Phe Arg Leu Arg Arg Pro Leu Gln Ser Ala Pro Arg Arg Trp Tyr
1 5 10 15
Ser Glu Thr His Lys Val Asn Asp Arg Val Val Val Leu Ser Asn Asp
20 25 30
Ala Thr Ser His Gln Thr Pro Val Leu Leu Gly Leu Met Asn Thr Leu
35 40 45
Ala Ile Lys Tyr His Ser Val Gly Tyr Phe Arg Pro Ile Ala Pro Pro
50 55 60
Val Gly Ser Asp His His Val Glu Leu Phe Lys Ser Glu Leu Lys Leu
65 70 75 80
Pro Glu Thr Tyr Glu Gln Leu Val Gly Leu His His Asp Asp Val Val
85 90 95
Asn Ala Arg Leu Ser Gly Asp Leu Asp Val Val Thr Asp Thr Ile Val
100 105 110
Ala Lys Phe Glu Ala Leu Arg Ala Lys His Asp Phe Val Val Ile Glu
115 120 125
Gly Ala Thr Phe Glu Ser Ala Pro Asp Leu Ala Trp Asp Ile Asn Val
130 135 140
Glu Leu Ala Lys Thr Leu Gly Ala Pro Val Leu Leu Thr Asn Asp Phe
145 150 155 160
Gly Asp Val Pro Glu Val Gln Arg Ile Glu Asp Ala Ile Ala Thr Arg
165 170 175
Val Leu Leu Gly Lys Asp Ala Val Asp Ala Ala Gly Leu Thr Tyr Ile
180 185 190
Gly Ser Ile Ala Asn Arg Val Arg Ser Ser Thr Pro Leu Glu Thr Arg
195 200 205
Gln Arg Val His Asp Leu Leu Arg Glu Lys Gly Ala Ala Asp Pro Thr
210 215 220
Ile Phe Leu Gly Ala Leu Pro Leu Asp Ser Ile Leu Ala Ser Lys Arg
225 230 235 240
Leu Asn Glu Val Val Ala Gln Leu Gln Ala Thr Gln Leu Tyr Gly Pro
245 250 255
Ala Ser Pro Asn Ser Val Val Val Thr Glu Gly Leu Ile Gly Thr Ser
260 265 270
Asp Leu Lys Glu Leu Phe Gly His Leu Lys Ala His Asp Asp Gly Leu
275 280 285
Leu Val Ile Thr Ser Ala Asp Arg Thr Asp Val Val Leu Gly Leu Leu
290 295 300
Ala Ser Arg Ala Ser Gly Ala Leu Pro Asn Val Ala Gly Val Val Leu
305 310 315 320
Thr Asn Gly Ala Tyr Pro Gln Asp His Val Lys Asn Ile Leu Asp Gly
325 330 335
Met Ala Lys Ile Asp Asn Ala Thr Ile Pro Ile Tyr Thr Val Glu Gly
340 345 350
Asp Ala Tyr Lys Thr Ala Asn Ala Leu Ser Arg Val Thr Cys Asp Ile
355 360 365
Leu Pro Thr Ser Gln Thr Lys Ile Gln Gln Ser Asn Ile Leu Phe Asp
370 375 380
Lys Phe Val Ser Arg Ser Ala Leu Met Asp Thr Val Cys Gln Ala Val
385 390 395 400
Lys Ser Thr Lys Arg Thr Pro Lys Gln Phe Lys His Phe Leu Phe Ser
405 410 415
Lys Ala Arg Lys Val Gln Gln His Ile Val Leu Thr Glu Gly Glu Asp
420 425 430
Asp Arg Ile Leu Gln Ala Ala Asp Glu Val Leu Arg Arg Asp Ile Ala
435 440 445
Lys Leu Thr Ile Leu Gly Asp Val Glu Ser Ile Ala Ala Arg Ala Lys
450 455 460
Thr Leu Arg Leu Asp Leu Ser Ala Ala Ser Ile Val Asp Pro Ser Lys
465 470 475 480
Ser Ala Asp Leu Asp Leu Leu Ala Ala Arg Phe Tyr Glu Lys Arg Lys
485 490 495
Val Lys Gly Val Ser Leu Glu Phe Ala Arg Glu Ser Ala Ala Glu Ala
500 505 510
Thr Cys Tyr Gly Thr Leu Met Val Glu Met Gly Leu Ala Asp Gly Met
515 520 525
Val Ser Gly Ala Cys His Thr Thr Ala Asn Thr Val Arg Pro Ala Leu
530 535 540
Gln Leu Ile Lys Thr Arg Pro Asp Arg Pro Leu Val Ser Ser Val Phe
545 550 555 560
Phe Met Cys Leu Glu Asp Asp Val Val Val Tyr Gly Asp Cys Ala Ile
565 570 575
Asn Thr Asp Pro Thr Ala Glu Asp Leu Ala Asn Ile Ala Val Gln Ser
580 585 590
Ala Glu Ser Ala Ile Ala Phe Gly Met Glu Pro Arg Val Ala Leu Leu
595 600 605
Ser Tyr Ala Thr Gly Asp Ser Asn Lys Gly Pro Ile Ile Asp Lys Val
610 615 620
Arg Glu Ala Thr Lys Leu Ala Gln Lys Met Ala Pro Glu Ile Pro Met
625 630 635 640
Tyr Gly Pro Ile Gln Tyr Asp Ala Ala Met Asn Pro Leu Ile Ala Lys
645 650 655
Gln Lys Val Lys Gly Leu Lys Lys Thr Glu Met Glu Val Ala Gly Asn
660 665 670
Ala Asn Val Leu Ile Phe Pro Asp Leu Asn Thr Gly Asn Asn Thr Tyr
675 680 685
Lys Ala Val Gln Gln Ser Thr Asn Cys Leu Ala Met Gly Pro Met Leu
690 695 700
Gln Gly Leu Asn Lys Pro Val Asn Asp Leu Ser Arg Gly Ala Thr Val
705 710 715 720
Gly Asp Ile Val Thr Thr Ile Ala Ile Thr Ala Ile Gln Ala Asp Gln
725 730 735
Met Arg Thr Ala Ala Ser Leu
740
<210> 110
<211> 2232
<212> DNA
<213> Saprolegnia parasitica
<400> 110
atgttccgac tgcgacgacc cctgcagtcc gccccccgac gatggtactc cgagacccac 60
aaggtcaacg accgagtcgt cgtcctgtcc aacgacgcca cctcccacca gacccccgtc 120
ctgctgggcc tgatgaacac cctggccatc aagtaccact ccgtcggcta cttccgaccc 180
atcgcccccc ccgtcggctc cgaccaccac gtcgagctgt tcaagtccga gctgaagctg 240
cccgagacct acgagcagct ggtcggcctg caccacgacg acgtcgtcaa cgcccgactg 300
tccggcgacc tggacgtcgt caccgacacc atcgtcgcca agttcgaggc cctgcgagcc 360
aagcacgact tcgtcgtcat cgagggcgcc accttcgagt ccgcccccga cctggcctgg 420
gacatcaacg tcgagctggc caagaccctg ggcgcccccg tcctgctgac caacgacttc 480
ggcgacgtcc ccgaggtcca gcgaatcgag gacgccatcg ccacccgagt cctgctgggc 540
aaggacgccg tcgacgccgc cggcctgacc tacatcggct ccatcgccaa ccgagtccga 600
tcctccaccc ccctggagac ccgacagcga gtccacgacc tgctgcgaga gaagggcgcc 660
gccgacccca ccatcttcct gggcgccctg cccctggact ccatcctggc ctccaagcga 720
ctgaacgagg tcgtcgccca gctgcaggcc acccagctgt acggccccgc ctcccccaac 780
tccgtcgtcg tcaccgaggg cctgatcggc acctccgacc tgaaggagct gttcggccac 840
ctgaaggccc acgacgacgg cctgctggtc atcacctccg ccgaccgaac cgacgtcgtc 900
ctgggcctgc tggcctcccg agcctccggc gccctgccca acgtcgccgg cgtcgtcctg 960
accaacggcg cctaccccca ggaccacgtc aagaacatcc tggacggcat ggccaagatc 1020
gacaacgcca ccatccccat ctacaccgtc gagggcgacg cctacaagac cgccaacgcc 1080
ctgtcccgag tcacctgcga catcctgccc acctcccaga ccaagatcca gcagtccaac 1140
atcctgttcg acaagttcgt ctcccgatcc gccctgatgg acaccgtctg ccaggccgtc 1200
aagtccacca agcgaacccc caagcagttc aagcacttcc tgttctccaa ggcccgaaag 1260
gtccagcagc acatcgtcct gaccgagggc gaggacgacc gaatcctgca ggccgccgac 1320
gaggtcctgc gacgagacat cgccaagctg accatcctgg gcgacgtcga gtccatcgcc 1380
gcccgagcca agaccctgcg actggacctg tccgccgcct ccatcgtcga cccctccaag 1440
tccgccgacc tggacctgct ggccgcccga ttctacgaga agcgaaaggt caagggcgtc 1500
tccctggagt tcgcccgaga gtccgccgcc gaggccacct gctacggcac cctgatggtc 1560
gagatgggcc tggccgacgg catggtctcc ggcgcctgcc acaccaccgc caacaccgtc 1620
cgacccgccc tgcagctgat caagacccga cccgaccgac ccctggtctc ctccgtcttc 1680
ttcatgtgcc tggaggacga cgtcgtcgtc tacggcgact gcgccatcaa caccgacccc 1740
accgccgagg acctggccaa catcgccgtc cagtccgccg agtccgccat cgccttcggc 1800
atggagcccc gagtcgccct gctgtcctac gccaccggcg actccaacaa gggccccatc 1860
atcgacaagg tccgagaggc caccaagctg gcccagaaga tggcccccga gatccccatg 1920
tacggcccca tccagtacga cgccgccatg aaccccctga tcgccaagca gaaggtcaag 1980
ggcctgaaga agaccgagat ggaggtcgcc ggcaacgcca acgtcctgat cttccccgac 2040
ctgaacaccg gcaacaacac ctacaaggcc gtccagcagt ccaccaactg cctggccatg 2100
ggccccatgc tgcagggcct gaacaagccc gtcaacgacc tgtcccgagg cgccaccgtc 2160
ggcgacatcg tcaccaccat cgccatcacc gccatccagg ccgaccagat gcgaaccgcc 2220
gcctccctgt aa 2232
<210> 111
<211> 343
<212> PRT
<213> Selaginella moellendorffii
<400> 111
Phe Arg Asn Ser Leu Phe Ser Arg Ala Lys Glu Asn Asn Gln Val Ile
1 5 10 15
Leu Leu Pro Glu Gly Asp Glu Pro Arg Thr Val Gln Ala Ala Gly Phe
20 25 30
Ile Leu Gln His Gly Leu Cys Ser Leu Ile Leu Leu Gly Glu Arg Glu
35 40 45
Lys Leu Leu Glu Ala Ala Lys Val Tyr Asn Val Asp Leu Arg Ser Ala
50 55 60
Ile Ile Lys Asp Pro Ser Asp Pro Gln Glu Leu Glu Lys Tyr Ala Thr
65 70 75 80
Val Tyr Tyr Gln Thr Arg Lys His Lys Gly Met Thr Leu Glu Lys Ala
85 90 95
Arg Glu Ile Leu Gly Asn Asp Pro Ile Thr Leu Gly Thr Cys Met Val
100 105 110
Ser Ala Gly Asp Ala Asp Gly Met Val Cys Gly Ala Val His Thr Thr
115 120 125
Ala Asn Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Asp Pro Ala
130 135 140
Thr Pro Ile Val Ser Ser Val Met Phe Ile Cys Leu Glu Asp Ala Val
145 150 155 160
Val Ala Tyr Ala Asp Val Ala Ile Asn Ala Ser Pro Ser Ala Asp Glu
165 170 175
Leu Ala Thr Ile Ala Ile Ala Ser Ala Asp Thr Val Thr Ala Phe Gly
180 185 190
Leu Glu Pro Arg Val Ala Leu Leu Ser Tyr Ala Thr Gly Asp Ser Asn
195 200 205
Ala Gly Pro Leu Val Gln Lys Val Ala Asp Ala Ala Ser Ile Ala Arg
210 215 220
Ser Arg Arg Pro Asp Leu Leu Leu Glu Gly Pro Phe Gln Tyr Asp Ala
225 230 235 240
Ala Val Asn Ala Ala Ala Ala Lys Ile Lys Leu Lys Gly Lys Asn Ser
245 250 255
Glu Val Ala Gly Lys Ala Asn Val Phe Ile Phe Pro Asp Leu Asn Ser
260 265 270
Ser Asn Ile Ala Cys Lys Val Val Gln Gln His Thr Gly Ala Thr Val
275 280 285
Ile Gly Pro Ile Leu Gln Gly Leu Arg Lys Pro Val Asn Asp Leu Ser
290 295 300
Arg Gly Cys Thr Val Lys Asp Ile Ile Ala Thr Ile Ala Thr Thr Ala
305 310 315 320
Ile Gln Ala Ala Ala Glu Lys Lys Lys Ser Lys Ala Val Ala Val Glu
325 330 335
Ala Met Lys Ala Arg Lys Glu
340
<210> 112
<211> 1032
<212> DNA
<213> Selaginella moellendorffii
<400> 112
ttccgaaact ccctgttctc ccgagccaag gagaacaacc aggtcatcct gctgcccgag 60
ggcgacgagc cccgaaccgt ccaggccgcc ggcttcatcc tgcagcacgg cctgtgctcc 120
ctgatcctgc tgggcgagcg agagaagctg ctggaggccg ccaaggtcta caacgtcgac 180
ctgcgatccg ccatcatcaa ggacccctcc gacccccagg agctggagaa gtacgccacc 240
gtctactacc agacccgaaa gcacaagggc atgaccctgg agaaggcccg agagatcctg 300
ggcaacgacc ccatcaccct gggcacctgc atggtctccg ccggcgacgc cgacggcatg 360
gtctgcggcg ccgtccacac caccgccaac accgtccgac ccgccctgca gatcatcaag 420
accgaccccg ccacccccat cgtctcctcc gtcatgttca tctgcctgga ggacgccgtc 480
gtcgcctacg ccgacgtcgc catcaacgcc tccccctccg ccgacgagct ggccaccatc 540
gccatcgcct ccgccgacac cgtcaccgcc ttcggcctgg agccccgagt cgccctgctg 600
tcctacgcca ccggcgactc caacgccggc cccctggtcc agaaggtcgc cgacgccgcc 660
tccatcgccc gatcccgacg acccgacctg ctgctggagg gccccttcca gtacgacgcc 720
gccgtcaacg ccgccgccgc caagatcaag ctgaagggca agaactccga ggtcgccggc 780
aaggccaacg tcttcatctt ccccgacctg aactcctcca acatcgcctg caaggtcgtc 840
cagcagcaca ccggcgccac cgtcatcggc cccatcctgc agggcctgcg aaagcccgtc 900
aacgacctgt cccgaggctg caccgtcaag gacatcatcg ccaccatcgc caccaccgcc 960
atccaggccg ccgccgagaa gaagaagtcc aaggccgtcg ccgtcgaggc catgaaggcc 1020
cgaaaggagt aa 1032
<210> 113
<211> 329
<212> PRT
<213> Gluconacetobacter diazotrophicus
<400> 113
Met Met Ala Pro Pro Thr Pro Gly His Gly Met Thr Asp Glu Val Thr
1 5 10 15
Asp Ala Lys Met Asp Ala Met Ile Ala Gly Pro Gly Phe Leu Asp Thr
20 25 30
Leu Ser Ala Arg Leu Ala Gly Ser Pro Pro Val Asp Ala Ala Val Ile
35 40 45
Tyr Pro Cys Ser Leu Pro Ser Leu Gln Ala Ala Val Gly Leu Gly Gln
50 55 60
Gln Gly Ile Val Gln Pro Ile Leu Ile Gly Pro Gly Glu Arg Ile Arg
65 70 75 80
Ala Leu Ala Arg Ser Ala Ser Leu Asp Leu Ala Ala Cys Arg Leu Val
85 90 95
Glu Ala Ala Asp Glu His Leu Ala Ala Ala Arg Gly Val Ala Leu Ala
100 105 110
Arg Asp Gly Thr Ala Arg Met Leu Met Lys Gly Ser Leu His Ser Ser
115 120 125
Ile Phe Leu Arg Glu Ile Gly His His Glu Ser Gly Leu Arg Thr Asp
130 135 140
Arg Arg Met Ser His Val Phe Val Leu Asp Val Pro Thr Cys Ser Arg
145 150 155 160
Pro Leu Leu Val Thr Asp Gly Ala Val Asn Ile Ala Pro Asp Leu Pro
165 170 175
Ala Arg Arg Asp Ile Val Gln Asn Ala Ile Asp Leu Ala Arg Thr Ile
180 185 190
Gly Ile Ser Arg Pro Arg Val Ala Ile Leu Ser Ala Ile Glu Thr Val
195 200 205
Asn Pro Glu Leu Pro Ser Thr Val Asp Ala Ala Leu Leu Ala Lys Met
210 215 220
Ala Glu Arg Gly Gln Ile Thr Gly Gly Ile Val Asp Gly Pro Leu Ala
225 230 235 240
Leu Asp Asn Ala Leu Ser Ala Glu Ala Ala Arg Cys Lys Gly Val Glu
245 250 255
Ser Pro Val Ala Gly Cys Ala Asp Ile Leu Val Val Pro Asp Leu Glu
260 265 270
Ala Gly Asn Met Leu Ala Lys Gln Leu Thr Phe Met Gly Gly Ala Ser
275 280 285
Ala Ala Gly Val Val Leu Gly Ala Arg Val Pro Val Val Leu Thr Ser
290 295 300
Arg Ala Asp Ser Val Arg Thr Arg Ile Leu Ser Gly Ile Leu Ala Ala
305 310 315 320
Val Leu Ala Gln Ala Arg Gly Met Asp
325
<210> 114
<211> 990
<212> DNA
<213> Gluconacetobacter diazotrophicus
<400> 114
atgatggctc ctcctacacc tggtcatggt atgacagatg aagtaacaga tgctaaaatg 60
gatgctatga tagctggtcc tggtttttta gatacattat cagctagatt agctggttca 120
cctcctgtag atgctgctgt aatatatcct tgttcattac cttcattaca agctgctgta 180
ggtttaggtc aacaaggtat agtacaacct atattaatag gtcctggtga aagaataaga 240
gctttagcta gatcagcttc attagattta gctgcttgta gattagtaga agctgctgat 300
gaacatttag ctgctgctag aggtgtagct ttagctagag atggtacagc tagaatgtta 360
atgaaaggtt cattacattc atcaatattt ttaagagaaa taggtcatca tgaatcaggt 420
ttaagaacag atagaagaat gtcacatgta tttgtattag atgtacctac atgttcaaga 480
cctttattag taacagatgg tgctgtaaat atagctcctg atttacctgc tagaagagat 540
atagtacaaa atgctataga tttagctaga acaataggta tatcaagacc tagagtagct 600
atattatcag ctatagaaac agtaaatcct gaattacctt caacagtaga tgctgcttta 660
ttagctaaaa tggctgaaag aggtcaaata acaggtggta tagtagatgg tcctttagct 720
ttagataatg ctttatcagc tgaagctgct agatgtaaag gtgtagaatc acctgtagct 780
ggttgtgctg atatattagt agtacctgat ttagaagctg gtaatatgtt agctaaacaa 840
ttaacattta tgggtggtgc ttcagctgct ggtgtagtat taggtgctag agtacctgta 900
gtattaacat caagagctga ttcagtaaga acaagaatat tatcaggtat attagctgct 960
gtattagctc aagctagagg tatggattga 990
<210> 115
<211> 953
<212> PRT
<213> Yarrowia lipolytica
<400> 115
Met Ile Glu Gly Ile Ser Phe Ala Ser Phe Val Thr His Glu Lys Pro
1 5 10 15
Lys Phe Val Arg Ala Leu Asp Phe Tyr Lys Ala Leu Gly Phe Leu Pro
20 25 30
Thr Lys Glu Tyr Lys His Gly Thr Asp His His Ala Thr Asp Glu Glu
35 40 45
Gly Ala Gly Ser Ile Gln Glu Val Trp Leu Thr Ser Ser Arg Ala Gly
50 55 60
Val Pro Ser Val Thr Val Lys Leu Arg Leu Ser Arg His Gly Asn Glu
65 70 75 80
His Val Ser Leu Pro Asn Leu Lys His Asp Trp Arg Ser Leu Val Pro
85 90 95
Ser Leu Val Tyr Tyr Ala Pro Asp Leu Asp Ala Val Arg Ala Ala Ile
100 105 110
Thr Pro Phe Leu His Glu Asp His Ser Thr Leu Leu Glu Arg Pro Ser
115 120 125
His Thr Asn Phe Ile Glu Leu Tyr Ala Ile Asp Pro Met Gly Asn Leu
130 135 140
Val Gly Phe Ser Arg Arg Glu Asn Pro Tyr Ser Ser Ala Met Gln Lys
145 150 155 160
Pro Phe Ser Ala Asp Asp Ile Gly Pro Gln Asn Phe Ser Lys Pro Asn
165 170 175
Glu Thr Lys Ile Lys Gly Lys Lys Arg Ile Gly Val Met Thr Ser Gly
180 185 190
Gly Asp Ala Pro Gly Met Cys Ala Ala Val Arg Ala Val Val Arg Ala
195 200 205
Gly Ile Ala Arg Gly Cys Glu Val Tyr Ala Val Arg Glu Gly Tyr Glu
210 215 220
Gly Leu Val Lys Gly Gly Asp Leu Ile Glu Pro Leu Ser Trp Glu Asp
225 230 235 240
Val Arg Gly Trp Leu Ser Leu Gly Gly Thr Leu Ile Gly Thr Ala Arg
245 250 255
Cys Lys Glu Phe Arg Glu Arg Glu Gly Arg Leu Ala Gly Ala Leu Asn
260 265 270
Met Val Lys Asn Gly Ile Asp Ala Leu Ile Val Ile Gly Gly Asp Gly
275 280 285
Ser Leu Thr Gly Ala Asp Leu Phe Arg Glu Glu Trp Pro Ser Leu Ile
290 295 300
Glu Glu Leu Val Thr Asn Gly Ser Ile Thr Ala Glu Gln Ala Glu Arg
305 310 315 320
His Arg His Leu Asp Ile Cys Gly Met Val Gly Ser Ile Asp Asn Asp
325 330 335
Met Ala Thr Thr Asp Val Thr Ile Gly Ala Tyr Ser Ser Leu Asp Arg
340 345 350
Ile Cys Glu Leu Val Asp Phe Ile Asp Ala Thr Ala Gln Ser His Ser
355 360 365
Arg Ala Phe Val Val Glu Val Met Gly Arg His Cys Gly Trp Leu Ala
370 375 380
Leu Met Ala Gly Thr Ala Thr Gly Ala Asp Tyr Ile Phe Ile Pro Glu
385 390 395 400
Ala Ala Pro Asp Ala Thr Gln Trp Ala Glu Lys Met Thr Arg Val Val
405 410 415
Lys Arg His Arg Ser Gln Gly Lys Arg Lys Thr Val Val Ile Val Ala
420 425 430
Glu Gly Ala Ile Asp Ser Asp Leu Asn Pro Ile Thr Ala Lys Met Val
435 440 445
Lys Asp Val Leu Asp Gly Ile Gly Leu Asp Thr Arg Ile Ser Thr Leu
450 455 460
Gly His Val Gln Arg Gly Gly Pro Pro Val Ala Ala Asp Arg Val Leu
465 470 475 480
Ala Ser Leu Gln Gly Val Glu Ala Ile Asp Ala Ile Leu Ser Leu Thr
485 490 495
Pro Glu Thr Pro Ser Pro Met Ile Ala Leu Asn Glu Asn Lys Ile Thr
500 505 510
Arg Lys Pro Leu Val Glu Ser Val Ala Leu Thr Lys Lys Val Ala Asp
515 520 525
Ala Ile Gly Asn Lys Asp Phe Ala Glu Ala Met Arg Leu Arg Asn Pro
530 535 540
Glu Phe Val Glu Gln Leu Gln Gly Phe Leu Leu Thr Asn Ser Ala Asp
545 550 555 560
Lys Asp Arg Pro Gln Glu Pro Ala Lys Asp Pro Leu Arg Val Ala Ile
565 570 575
Val Cys Thr Gly Ala Pro Ala Gly Gly Met Asn Ala Ala Ile Arg Ser
580 585 590
Ala Val Leu Tyr Gly Leu Ala Arg Gly His Gln Met Phe Ala Ile His
595 600 605
Asn Gly Trp Ser Gly Leu Val Lys Asn Gly Asp Asp Ala Val Arg Glu
610 615 620
Leu Thr Trp Leu Glu Val Glu Pro Leu Cys Gln Lys Gly Gly Cys Glu
625 630 635 640
Ile Gly Thr Asn Arg Ser Leu Pro Glu Cys Asp Leu Gly Met Ile Ala
645 650 655
Tyr His Phe Gln Arg Gln Arg Phe Asp Gly Leu Ile Val Ile Gly Gly
660 665 670
Phe Glu Ala Phe Arg Ala Leu Asn Gln Leu Asp Asp Ala Arg His Ala
675 680 685
Tyr Pro Ala Leu Arg Ile Pro Met Val Gly Ile Pro Ala Thr Ile Ser
690 695 700
Asn Asn Val Pro Gly Thr Asp Tyr Ser Leu Gly Ala Asp Thr Cys Leu
705 710 715 720
Asn Ser Leu Val Gln Tyr Cys Asp Val Leu Lys Thr Ser Ala Ser Ala
725 730 735
Thr Arg Leu Arg Leu Phe Val Val Glu Val Gln Gly Gly Asn Ser Gly
740 745 750
Tyr Ile Ala Thr Val Ala Gly Leu Ile Thr Gly Ala Tyr Val Val Tyr
755 760 765
Thr Pro Glu Ser Gly Ile Asn Leu Arg Leu Leu Gln His Asp Ile Ser
770 775 780
Tyr Leu Lys Asp Thr Phe Ala His Gln Ala Asp Val Asn Arg Thr Gly
785 790 795 800
Lys Leu Leu Leu Arg Asn Glu Arg Ser Ser Asn Val Phe Thr Thr Asp
805 810 815
Val Ile Thr Gly Ile Ile Asn Glu Glu Ala Lys Gly Ser Phe Asp Ala
820 825 830
Arg Thr Ala Ile Pro Gly His Val Gln Gln Gly Gly His Pro Ser Pro
835 840 845
Thr Asp Arg Val Arg Ala Gln Arg Phe Ala Ile Lys Ala Val Gln Phe
850 855 860
Ile Glu Glu His His Gly Ser Lys Asn Asn Ala Asp His Cys Val Ile
865 870 875 880
Leu Gly Val Arg Gly Ser Lys Phe Lys Tyr Thr Ser Val Ser His Leu
885 890 895
Tyr Ala His Lys Thr Glu His Gly Ala Arg Arg Pro Lys His Ser Tyr
900 905 910
Trp His Ala Ile Gly Asp Ile Ala Asn Met Leu Val Gly Arg Lys Ala
915 920 925
Pro Pro Leu Pro Glu Thr Leu Asn Asp Glu Ile Glu Lys Asn Ile Ala
930 935 940
Lys Glu Gln Gly Ile Ile Asp Pro Cys
945 950
<210> 116
<211> 972
<212> DNA
<213> Bacillus subtilis
<400> 116
atggcagatt tattttcaac agtgcaagaa aaagtagctg gaaaagacgt taaaattgta 60
tttcctgaag gcttagacga gcgtatttta gaagcggtca gcaagcttgc aggaaacaaa 120
gtgctgaatc cgattgtgat cggcaatgaa aatgagatcc aagcaaaagc aaaagaattg 180
aaccttacgc tgggcggcgt taagatttat gatcctcata catatgaagg catggaagac 240
cttgtacaag cattcgtaga acgccgcaaa ggcaaagcga ctgaagaaca ggctcgtaaa 300
gcattattag acgagaacta cttcggtaca atgctggtgt ataaaggact tgcagacgga 360
cttgtaagcg gagctgctca ctcaactgct gacactgtcc gcccggctct tcaaatcatc 420
aaaacaaaag aaggcgtgaa aaagacttca ggcgtgttca tcatggctcg cggagaagag 480
caatacgtat tcgcagattg cgcgatcaac attgcacctg acagccaaga tcttgccgag 540
attgcgatcg aaagtgccaa tacggcaaaa atgttcgaca ttgagcctcg cgtggcaatg 600
ctcagcttct ctacaaaagg ctcagcaaaa tctgatgaaa cagaaaaagt agcggatgca 660
gtgaaaatcg cgaaagaaaa agcgcctgaa ctgacacttg acggcgaatt ccaatttgat 720
gctgcatttg ttccatctgt agctgagaaa aaagcgccgg attccgagat caaaggggac 780
gctaacgtat tcgtattccc aagtcttgaa gcaggaaaca tcggctataa aatcgctcag 840
cgtttgggca actttgaagc ggtaggacca atcctgcaag gtttaaatat gcctgtaaac 900
gacctttcaa gaggatgtaa cgctgaagat gtttacaatc tcgcattaat tacagcggcg 960
caagcactgt aa 972
<210> 117
<211> 333
<212> PRT
<213> Clostridium acetobutylicum
<400> 117
Met Asp Leu Ile Glu Ser Ile Trp Glu Cys Ala Lys Gln Asp Lys Lys
1 5 10 15
Arg Ile Ile Leu Ala Glu Gly Glu Glu Lys Arg Asn Leu Ile Ala Ala
20 25 30
Asp Lys Ile Ile Lys Glu Gly Leu Ala Glu Leu Val Leu Val Gly Asp
35 40 45
Glu Asn Lys Ile Lys Glu Lys Ala Ser Glu Leu Asn Leu Asp Ile Ser
50 55 60
Lys Ala Glu Ile Met Asp Pro Glu Thr Ser Leu Lys Thr Glu Thr Tyr
65 70 75 80
Ala Arg Asp Phe Tyr Glu Leu Arg Lys His Lys Gly Met Thr Ile Glu
85 90 95
Lys Ser Glu Lys Met Val Arg Asp Pro Leu Tyr Phe Ala Thr Met Ala
100 105 110
Leu Lys Asp Gly Tyr Val Asp Gly Met Val Ser Gly Ala Val His Thr
115 120 125
Thr Gly Asp Leu Leu Arg Pro Gly Leu Gln Ile Ile Lys Thr Ala Pro
130 135 140
Gly Val Lys Ile Val Ser Gly Phe Phe Val Met Ile Ile Pro Asp Cys
145 150 155 160
Asp Tyr Gly Glu Glu Gly Leu Leu Leu Phe Ala Asp Cys Ala Val Asn
165 170 175
Pro Asn Pro Thr Ser Asp Glu Leu Ala Asp Ile Ala Ile Thr Thr Ala
180 185 190
Glu Thr Ala Arg Lys Leu Cys Asn Val Glu Pro Lys Val Ala Met Leu
195 200 205
Ser Phe Ser Thr Met Gly Ser Ala Lys Gly Glu Met Val Asp Lys Val
210 215 220
Lys Asn Ala Val Glu Ile Thr Lys Lys Phe Arg Pro Asp Leu Ala Ile
225 230 235 240
Asp Gly Glu Leu Gln Leu Asp Ala Ala Ile Asp Ser Glu Val Ala Ala
245 250 255
Leu Lys Ala Pro Ser Ser Asn Val Ala Gly Asn Ala Asn Val Leu Val
260 265 270
Phe Pro Asp Leu Gln Thr Gly Asn Ile Gly Tyr Lys Leu Val Gln Arg
275 280 285
Phe Ala Lys Ala Lys Ala Ile Gly Pro Ile Cys Gln Gly Phe Ala Lys
290 295 300
Pro Ile Asn Asp Leu Ser Arg Gly Cys Ser Ser Glu Asp Ile Val Asn
305 310 315 320
Val Val Ala Ile Thr Val Val Gln Ala Gln Arg Gly Ile
325 330
<210> 118
<211> 1002
<212> DNA
<213> Clostridium acetobutylicum
<400> 118
atggatttaa tagaaagcat atgggagtgt gctaagcaag acaaaaaaag gataatatta 60
gctgaaggtg aagaaaaaag aaatctaatt gccgcagata aaattatcaa agagggatta 120
gcagagcttg ttcttgtagg tgatgaaaat aaaattaaag aaaaagcaag tgagttgaat 180
cttgacattt cgaaggctga aataatggat ccagagacat cactaaaaac agaaacatat 240
gctagagatt tttatgaact tagaaaacac aaaggaatga ctattgaaaa atctgaaaaa 300
atggtaagag atcctcttta ttttgcaaca atggctttaa aagatggcta tgttgatgga 360
atggtttcag gagctgttca cacaactgga gatttattaa gaccaggact tcaaattata 420
aaaactgcac caggagttaa aatagtatca ggattctttg ttatgataat acctgactgc 480
gattatggtg aagagggtct tttattattt gcagattgtg ctgtaaatcc taacccaaca 540
tcagatgaac tagctgatat tgctataact acagctgaaa cagctagaaa attatgtaac 600
gtagagccta aagttgcgat gctttcattc tcaactatgg gaagtgcaaa aggcgaaatg 660
gtagataagg ttaaaaatgc tgttgaaatc acaaagaaat tcagaccgga tcttgctatt 720
gatggtgagc ttcagcttga tgctgcaata gatagtgaag tagcggcttt aaaagcacct 780
tctagtaatg ttgcaggaaa tgcaaatgtt cttgtattcc cagatcttca aacaggaaac 840
attgggtaca agcttgttca aagatttgca aaagcaaaag caataggacc tatatgtcaa 900
ggatttgcaa aacctattaa tgatttatca agaggctgta gctcagagga tatagtaaat 960
gttgttgcta taactgttgt tcaggctcaa agaggtatat aa 1002
<210> 119
<211> 328
<212> PRT
<213> Thermoanaerobacterium saccharolyticum
<400> 119
Met Ser Ile Ile Gln Asn Ile Ile Glu Lys Ala Lys Ser Asp Lys Lys
1 5 10 15
Lys Ile Val Leu Pro Glu Gly Ala Glu Pro Arg Thr Leu Lys Ala Ala
20 25 30
Glu Ile Val Leu Lys Glu Gly Ile Ala Asp Leu Val Leu Leu Gly Asn
35 40 45
Glu Asp Glu Ile Arg Asn Ala Ala Lys Asp Leu Asp Ile Ser Lys Ala
50 55 60
Glu Ile Ile Asp Pro Val Lys Ser Glu Met Phe Asp Arg Tyr Ala Asn
65 70 75 80
Asp Phe Tyr Glu Leu Arg Lys Asn Lys Gly Ile Thr Leu Glu Lys Ala
85 90 95
Arg Glu Thr Ile Lys Asp Asn Ile Tyr Phe Gly Cys Met Met Val Lys
100 105 110
Glu Gly Tyr Ala Asp Gly Leu Val Ser Gly Ala Ile His Ala Thr Ala
115 120 125
Asp Leu Leu Arg Pro Ala Phe Gln Ile Ile Lys Thr Ala Pro Gly Ala
130 135 140
Lys Ile Val Ser Ser Phe Phe Ile Met Glu Val Pro Asn Cys Glu Tyr
145 150 155 160
Gly Glu Asn Gly Val Phe Leu Phe Ala Asp Cys Ala Val Asn Pro Ser
165 170 175
Pro Asn Ala Glu Glu Leu Ala Ser Ile Ala Val Gln Ser Ala Asn Thr
180 185 190
Ala Lys Asn Leu Leu Gly Phe Glu Pro Lys Val Ala Met Leu Ser Phe
195 200 205
Ser Thr Lys Gly Ser Ala Ser His Glu Leu Val Asp Lys Val Arg Lys
210 215 220
Ala Thr Glu Ile Ala Lys Glu Leu Met Pro Asp Val Ala Ile Asp Gly
225 230 235 240
Glu Leu Gln Leu Asp Ala Ala Leu Val Lys Glu Val Ala Glu Leu Lys
245 250 255
Ala Pro Gly Ser Lys Val Ala Gly Cys Ala Asn Val Leu Ile Phe Pro
260 265 270
Asp Leu Gln Ala Gly Asn Ile Gly Tyr Lys Leu Val Gln Arg Leu Ala
275 280 285
Lys Ala Asn Ala Ile Gly Pro Ile Thr Gln Gly Met Gly Ala Pro Val
290 295 300
Asn Asp Leu Ser Arg Gly Cys Ser Tyr Arg Asp Ile Val Asp Val Ile
305 310 315 320
Ala Thr Thr Ala Val Gln Ala Gln
325
<210> 120
<211> 987
<212> DNA
<213> Thermoanaerobacterium saccharolyticum
<400> 120
atgagcatta ttcaaaacat cattgaaaaa gctaaaagcg ataaaaagaa aattgttctg 60
ccagaaggtg cagaacccag gacattaaaa gctgctgaaa tagttttaaa agaagggatt 120
gcagatttag tgcttcttgg aaatgaagat gagataagaa atgctgcaaa agacttggac 180
atatccaaag ctgaaatcat tgaccctgta aagtctgaaa tgtttgatag gtatgctaat 240
gatttctatg agttaaggaa gaacaaagga atcacgttgg aaaaagccag agaaacaatc 300
aaggataata tctattttgg atgtatgatg gttaaagaag gttatgctga tggattggta 360
tctggcgcta ttcatgctac tgcagattta ttaagacctg catttcagat aattaaaacg 420
gctccaggag caaagatagt atcaagcttt tttataatgg aagtgcctaa ttgtgaatat 480
ggtgaaaatg gtgtattctt gtttgctgat tgtgcggtca acccatcgcc taatgcagaa 540
gaacttgctt ctattgccgt acaatctgct aatactgcaa agaatttgtt gggctttgaa 600
ccaaaagttg ccatgctatc attttctaca aaaggtagtg catcacatga attagtagat 660
aaagtaagaa aagcgacaga gatagcaaaa gaattgatgc cagatgttgc tatcgacggt 720
gaattgcaat tggatgctgc tcttgttaaa gaagttgcag agctaaaagc gccgggaagc 780
aaagttgcgg gatgtgcaaa tgtgcttata ttccctgatt tacaagctgg taatatagga 840
tataagcttg tacagaggtt agctaaggca aatgcaattg gacctataac acaaggaatg 900
ggtgcaccgg ttaatgattt atcaagagga tgcagctata gagatattgt tgacgtaata 960
gcaacaacag ctgtgcaggc tcaataa 987
<210> 121
<211> 813
<212> PRT
<213> Leuconostoc mesenteroides
<400> 121
Met Ala Asp Phe Asp Ser Lys Glu Tyr Leu Glu Leu Val Asp Lys Trp
1 5 10 15
Trp Arg Ala Thr Asn Tyr Leu Ser Ala Gly Met Ile Phe Leu Lys Ser
20 25 30
Asn Pro Leu Phe Ser Val Thr Asn Thr Pro Ile Lys Ala Glu Asp Val
35 40 45
Lys Val Lys Pro Ile Gly His Trp Gly Thr Ile Ser Gly Gln Thr Phe
50 55 60
Leu Tyr Ala His Ala Asn Arg Leu Ile Asn Lys Tyr Gly Leu Asn Met
65 70 75 80
Phe Tyr Val Gly Gly Pro Gly His Gly Gly Gln Val Met Val Thr Asn
85 90 95
Ala Tyr Leu Asp Gly Ala Tyr Thr Glu Asp Tyr Pro Glu Ile Thr Gln
100 105 110
Asp Ile Glu Gly Met Ser His Leu Phe Lys Arg Phe Ser Phe Pro Gly
115 120 125
Gly Ile Gly Ser His Met Thr Ala Gln Thr Pro Gly Ser Leu His Glu
130 135 140
Gly Gly Glu Leu Gly Tyr Ser Leu Ser His Ala Phe Gly Ala Val Leu
145 150 155 160
Asp Asn Pro Asp Gln Val Ala Phe Ala Val Val Gly Asp Gly Glu Ala
165 170 175
Glu Thr Gly Pro Ser Met Ala Ser Trp His Ser Ile Lys Phe Leu Asn
180 185 190
Ala Lys Asn Asp Gly Ala Val Leu Pro Val Leu Asp Leu Asn Gly Phe
195 200 205
Lys Ile Ser Asn Pro Thr Ile Phe Ser Arg Met Ser Asp Glu Glu Ile
210 215 220
Thr Lys Phe Phe Glu Gly Leu Gly Tyr Ser Pro Arg Phe Ile Glu Asn
225 230 235 240
Asp Asp Ile His Asp Tyr Ala Thr Tyr His Gln Leu Ala Ala Asn Ile
245 250 255
Leu Asp Gln Ala Ile Glu Asp Ile Gln Ala Ile Gln Asn Asp Ala Arg
260 265 270
Glu Asn Gly Lys Tyr Gln Asp Gly Glu Ile Pro Ala Trp Pro Val Ile
275 280 285
Ile Ala Arg Leu Pro Lys Gly Trp Gly Gly Pro Thr His Asp Ala Ser
290 295 300
Asn Asn Pro Ile Glu Asn Ser Phe Arg Ala His Gln Val Pro Leu Pro
305 310 315 320
Leu Glu Gln His Asp Leu Ala Thr Leu Pro Glu Phe Glu Asp Trp Met
325 330 335
Asn Ser Tyr Lys Pro Glu Glu Leu Phe Asn Ala Asp Gly Ser Leu Lys
340 345 350
Asp Glu Leu Lys Ala Ile Ala Pro Lys Gly Asp Lys Arg Met Ser Ala
355 360 365
Asn Pro Ile Thr Asn Gly Gly Ala Asp Arg Ser Asp Leu Lys Leu Pro
370 375 380
Asn Trp Arg Glu Phe Ala Asn Asp Ile Asn Asp Asp Thr Arg Gly Lys
385 390 395 400
Glu Phe Ala Asp Ser Lys Arg Asn Met Asp Met Ala Thr Leu Ser Asn
405 410 415
Tyr Leu Gly Ala Val Ser Gln Leu Asn Pro Thr Arg Phe Arg Phe Phe
420 425 430
Gly Pro Asp Glu Thr Met Ser Asn Arg Leu Trp Gly Leu Phe Asn Val
435 440 445
Thr Pro Arg Gln Trp Met Glu Glu Ile Lys Glu Pro Gln Asp Gln Leu
450 455 460
Leu Ser Pro Thr Gly Arg Ile Ile Asp Ser Gln Leu Ser Glu His Gln
465 470 475 480
Ala Glu Gly Trp Leu Glu Gly Tyr Thr Leu Thr Gly Arg Val Gly Ile
485 490 495
Phe Ala Ser Tyr Glu Ser Phe Leu Arg Val Val Asp Thr Met Val Thr
500 505 510
Gln His Phe Lys Trp Leu Arg His Ala Ser Glu Gln Ala Trp Arg Asn
515 520 525
Asp Tyr Pro Ser Leu Asn Leu Ile Ala Thr Ser Thr Ala Phe Gln Gln
530 535 540
Asp His Asn Gly Tyr Thr His Gln Asp Pro Gly Met Leu Thr His Leu
545 550 555 560
Ala Glu Lys Lys Ser Asn Phe Ile Arg Glu Tyr Leu Pro Ala Asp Gly
565 570 575
Asn Ser Leu Leu Ala Val Gln Glu Arg Ala Phe Ser Glu Arg His Lys
580 585 590
Val Asn Leu Leu Ile Ala Ser Lys Gln Pro Arg Gln Gln Trp Phe Thr
595 600 605
Val Glu Glu Ala Glu Val Leu Ala Asn Glu Gly Leu Lys Ile Ile Asp
610 615 620
Trp Ala Ser Thr Ala Pro Ser Gly Asp Val Asp Ile Thr Phe Ala Ser
625 630 635 640
Ala Gly Thr Glu Pro Thr Ile Glu Thr Leu Ala Ala Leu Trp Leu Ile
645 650 655
Asn Gln Ala Phe Pro Asp Val Lys Phe Arg Tyr Val Asn Val Val Glu
660 665 670
Leu Leu Arg Leu Gln Lys Lys Ser Glu Pro Asn Met Asn Asp Glu Arg
675 680 685
Glu Leu Ser Ala Glu Glu Phe Asn Lys Tyr Phe Gln Ala Asp Thr Pro
690 695 700
Val Ile Phe Gly Phe His Ala Tyr Glu Asn Leu Ile Glu Ser Phe Phe
705 710 715 720
Phe Glu Arg Lys Phe Thr Gly Asp Val Tyr Val His Gly Tyr Arg Glu
725 730 735
Asp Gly Asp Ile Thr Thr Thr Tyr Asp Met Arg Val Tyr Ser His Leu
740 745 750
Asp Arg Phe His Gln Ala Lys Glu Ala Ala Glu Ile Leu Ser Ala Asn
755 760 765
Gly Lys Ile Asp Gln Ala Ala Ala Asp Thr Phe Ile Ala Lys Met Asp
770 775 780
Asp Thr Leu Ala Lys His Phe Gln Val Thr Arg Asn Glu Gly Arg Asp
785 790 795 800
Ile Glu Glu Phe Thr Asp Trp Thr Trp Ser Pro Leu Lys
805 810
<210> 122
<211> 2442
<212> DNA
<213> Leuconostoc mesenteroides
<400> 122
atggcagatt tcgattcaaa agagtacttg gaacttgttg ataagtggtg gcgcgcaact 60
aactatttgt cagctgggat gatctttttg aagagcaacc cattgttctc agttactaat 120
acacctatca aggctgaaga tgtaaaagtt aagccaatcg gacactgggg tactatctca 180
ggtcagacat tcttgtatgc acatgctaac cgtttgatca acaagtatgg tttgaacatg 240
ttttacgttg gtggccctgg tcacggtggc caagttatgg ttactaacgc ttacttagac 300
ggcgcatata ctgaagatta tcctgaaatc actcaagata tcgaaggtat gagccacttg 360
ttcaagcgtt tctcattccc tggcggtatt ggatcacaca tgacagctca aacacctggt 420
tcattacacg aaggtggtga attgggctat tcattgagcc acgcttttgg tgccgttttg 480
gacaatcctg accaagttgc tttcgcagtt gttggtgatg gtgaagctga aacaggtcct 540
tcaatggctt catggcactc aattaagttt ttgaatgcta agaatgatgg tgccgttttg 600
cctgtcttgg atttgaacgg attcaagatt tcaaacccaa ctatcttctc acgtatgagt 660
gatgaagaaa tcacaaagtt ctttgaaggt ttgggttatt cacctcgctt catcgaaaac 720
gatgatattc atgactacgc aacataccac caacttgcag caaacatttt ggatcaagct 780
attgaagata ttcaagctat tcaaaatgat gcacgtgaaa atggtaagta tcaagatggt 840
gaaatccctg catggccagt aattattgct cgcttgccaa agggctgggg tggaccaacg 900
cacgatgcaa gtaacaatcc tattgaaaac tcattccgtg cgcaccaagt gccattgcct 960
cttgaacaac acgatcttgc aacattgcct gaattcgaag actggatgaa ctcatacaag 1020
cctgaagaat tattcaatgc tgatggttct ttgaaggatg aattgaaagc tatcgctcct 1080
aagggtgaca agcgtatgtc agctaaccct attacaaatg gtggtgctga tcgttcagac 1140
ttgaagttgc ctaactggag agaattcgct aacgatatca atgatgatac acgtggtaag 1200
gaattcgctg atagcaagcg caatatggac atggcaacat tgtcaaacta cttgggtgct 1260
gtttcacaat tgaacccaac tcgtttccgc ttcttcggtc ctgatgaaac aatgtcaaac 1320
cgtttgtggg gattgttcaa tgttacacca cgtcaatgga tggaagaaat caaggaacca 1380
caagatcaat tattgagccc tacgggtcgc attattgatt cacaattgtc tgaacatcaa 1440
gctgaaggtt ggcttgaagg atatactttg actggtcgtg ttggaatctt cgcatcatac 1500
gagtcattct tgcgtgttgt cgatacaatg gttacgcaac acttcaagtg gttgcgtcac 1560
gcttcagaac aagcatggcg taatgactat ccatcattga acttgattgc aacttcaact 1620
gctttccaac aagatcacaa tggatatact caccaagatc caggtatgtt gactcacttg 1680
gctgaaaaga agtctaactt tattcgtgaa tatttgccag ctgatggtaa ctcattgttg 1740
gctgttcaag aacgtgcctt ctcagaacgt cataaggtta acttgttgat tgcttctaag 1800
caaccacgtc aacaatggtt tacagttgaa gaagctgaag tattggctaa cgaaggtttg 1860
aagatcattg attgggcttc tactgcacct tctggtgatg ttgatattac attcgcatct 1920
gctggtactg aaccaacaat tgaaactttg gctgctttgt ggttgattaa ccaagcattc 1980
ccagatgtta agttccgtta tgttaacgtt gttgaattac tacgtttgca aaagaagtca 2040
gaacctaaca tgaatgatga acgtgaatta tcagccgaag aattcaacaa gtatttccaa 2100
gctgatacac cagttatctt cggtttccat gcttatgaaa acttgattga atcattcttc 2160
ttcgaacgta agttcacggg tgatgtatac gttcatggat atcgtgaaga tggtgacatc 2220
acaacgacat atgatatgcg tgtatattca cacttggatc gcttccatca agctaaggaa 2280
gctgctgaaa tcttgtctgc aaatggtaag attgatcaag ctgctgctga tacattcatc 2340
gctaagatgg atgatacttt ggcaaagcat ttccaagtta ctcgtaacga aggtcgtgat 2400
atcgaagaat tcactgactg gacatggtca ccacttaagt aa 2442
<210> 123
<211> 822
<212> PRT
<213> Lactococcus lactis
<400> 123
Met Thr Glu Tyr Asn Ser Glu Ala Tyr Leu Lys Lys Leu Asp Lys Trp
1 5 10 15
Trp Arg Ala Ala Thr Tyr Leu Gly Ala Gly Met Ile Phe Leu Lys Glu
20 25 30
Asn Pro Leu Phe Ser Val Thr Gly Thr Pro Ile Lys Ala Glu Asn Leu
35 40 45
Lys Ala Asn Pro Ile Gly His Trp Gly Thr Val Ser Gly Gln Thr Phe
50 55 60
Leu Tyr Ala His Ala Asn Arg Leu Ile Asn Lys Tyr Asp Gln Lys Met
65 70 75 80
Phe Tyr Met Gly Gly Pro Gly His Gly Gly Gln Ala Met Val Val Pro
85 90 95
Ser Tyr Leu Asp Gly Ser Tyr Thr Glu Ala Tyr Pro Glu Ile Thr Gln
100 105 110
Asp Leu Glu Gly Met Ser Arg Leu Phe Lys Arg Phe Ser Phe Pro Gly
115 120 125
Gly Ile Gly Ser His Met Thr Ala Gln Thr Pro Gly Ser Leu His Glu
130 135 140
Gly Gly Glu Leu Gly Tyr Val Leu Ser His Ala Thr Gly Ala Ile Leu
145 150 155 160
Asp Gln Pro Glu Gln Ile Ala Phe Ala Val Val Gly Asp Gly Glu Ala
165 170 175
Glu Thr Gly Pro Leu Met Thr Ser Trp His Ser Ile Lys Phe Ile Asn
180 185 190
Pro Lys Asn Asp Gly Ala Ile Leu Pro Ile Leu Asp Leu Asn Gly Phe
195 200 205
Lys Ile Ser Asn Pro Thr Leu Phe Ala Arg Thr Ser Asp Val Asp Ile
210 215 220
Arg Lys Phe Phe Glu Gly Leu Gly Tyr Ser Pro Arg Tyr Ile Glu Asn
225 230 235 240
Asp Asp Ile His Asp Tyr Met Ala Tyr His Lys Leu Ala Ala Glu Val
245 250 255
Phe Asp Lys Ala Ile Glu Asp Ile His Gln Ile Gln Lys Asp Ala Arg
260 265 270
Glu Asp Asn Arg Tyr Gln Asn Gly Glu Ile Pro Ala Trp Pro Ile Val
275 280 285
Ile Ala Arg Leu Pro Lys Gly Trp Gly Gly Pro Arg Tyr Asn Asp Trp
290 295 300
Ser Gly Pro Lys Phe Asp Gly Lys Gly Met Pro Ile Glu His Ser Phe
305 310 315 320
Arg Ala His Gln Val Pro Leu Pro Leu Ser Ser Lys Asn Met Gly Thr
325 330 335
Leu Pro Glu Phe Val Lys Trp Met Thr Ser Tyr Gln Pro Glu Thr Leu
340 345 350
Phe Asn Ala Asp Gly Ser Leu Lys Glu Glu Leu Arg Asp Phe Ala Pro
355 360 365
Lys Gly Glu Met Arg Met Ala Ser Asn Pro Val Thr Asn Gly Gly Val
370 375 380
Asp Ser Ser Asn Leu Val Leu Pro Asp Trp Gln Glu Phe Ala Asn Pro
385 390 395 400
Ile Ser Glu Asn Asn Arg Gly Lys Leu Leu Pro Asp Thr Asn Asp Asn
405 410 415
Met Asp Met Asn Val Leu Ser Lys Tyr Phe Ala Glu Ile Val Lys Leu
420 425 430
Asn Pro Thr Arg Phe Arg Leu Phe Gly Pro Asp Glu Thr Met Ser Asn
435 440 445
Arg Phe Trp Glu Met Phe Lys Val Thr Asn Arg Gln Trp Met Gln Val
450 455 460
Ile Lys Asn Pro Asn Asp Glu Phe Ile Ser Pro Glu Gly Arg Ile Ile
465 470 475 480
Asp Ser Gln Leu Ser Glu His Gln Ala Glu Gly Trp Leu Glu Gly Tyr
485 490 495
Thr Leu Thr Gly Arg Thr Gly Ala Phe Ala Ser Tyr Glu Ser Phe Leu
500 505 510
Arg Val Val Asp Ser Met Leu Thr Gln His Phe Lys Trp Ile Arg Gln
515 520 525
Ala Ala Asp Gln Lys Trp Arg His Asp Tyr Pro Ser Leu Asn Val Ile
530 535 540
Ser Thr Ser Thr Val Phe Gln Gln Asp His Asn Gly Tyr Thr His Gln
545 550 555 560
Asp Pro Gly Met Leu Thr His Leu Ala Glu Lys Lys Ser Asp Phe Ile
565 570 575
Arg Gln Tyr Leu Pro Ala Asp Gly Asn Thr Leu Leu Ala Val Phe Asp
580 585 590
Arg Ala Phe Gln Asp Arg Ser Lys Ile Asn His Ile Val Ala Ser Lys
595 600 605
Gln Pro Arg Gln Gln Trp Phe Thr Lys Glu Glu Ala Glu Lys Leu Ala
610 615 620
Thr Asp Gly Ile Ala Thr Ile Asp Trp Ala Ser Thr Ala Lys Asp Gly
625 630 635 640
Glu Ala Val Asp Leu Val Phe Ala Ser Ala Gly Ala Glu Pro Thr Ile
645 650 655
Glu Thr Leu Ala Ala Leu His Leu Val Asn Glu Val Phe Pro Gln Ala
660 665 670
Lys Phe Arg Tyr Val Asn Val Val Glu Leu Gly Arg Leu Gln Lys Lys
675 680 685
Lys Gly Ala Leu Asn Gln Glu Arg Glu Leu Ser Asp Glu Glu Phe Glu
690 695 700
Lys Tyr Phe Gly Pro Ser Gly Thr Pro Val Ile Phe Gly Phe His Gly
705 710 715 720
Tyr Glu Asp Leu Ile Glu Ser Ile Phe Tyr Gln Arg Gly His Asp Gly
725 730 735
Leu Ile Val His Gly Tyr Arg Glu Asp Gly Asp Ile Thr Thr Thr Tyr
740 745 750
Asp Met Arg Val Tyr Ser Glu Leu Asp Arg Phe His Gln Ala Ile Asp
755 760 765
Ala Met Gln Val Leu Tyr Val Asn Arg Lys Val Asn Gln Gly Leu Ala
770 775 780
Lys Ala Phe Ile Asp Arg Met Lys Arg Thr Leu Val Lys His Phe Glu
785 790 795 800
Val Thr Arg Asn Glu Gly Val Asp Ile Pro Asp Phe Thr Glu Trp Val
805 810 815
Trp Ser Asp Leu Lys Lys
820
<210> 124
<211> 2469
<212> DNA
<213> Lactococcus lactis
<400> 124
atgacagaat ataattcaga agcttatttg aaaaagcttg ataaatggtg gcgagcagca 60
acttatcttg gagcaggaat gatcttcttg aaagaaaatc cattgttctc tgtgacaggt 120
actccaatta aagcggaaaa ccttaaagcc aatcctattg ggcactgggg gacggtttca 180
ggacaaactt tcctctatgc tcatgctaat cgtctaatca ataaatatga tcaaaagatg 240
ttttacatgg gtggccccgg acatggtgga caagctatgg ttgttccttc ttatcttgat 300
ggctcatata cagaagctta tccagagatt acccaagatt tggaaggaat gtcacgtttg 360
tttaaacgtt tctcatttcc tggaggaata gggtcgcata tgacagcaca aacccctggt 420
tcacttcatg aaggaggtga gttgggttat gtgctatcac atgcaacagg ggctattctt 480
gatcaacctg aacagattgc ttttgctgtt gttggggatg gagaagctga aactggaccg 540
ttgatgacaa gttggcactc tattaaattc attaatccta agaatgatgg ggcgatttta 600
ccaattcttg atttaaatgg ttttaaaatt tcaaatccta ctttgttcgc tcgaacttca 660
gatgttgata ttcgtaaatt ctttgaagga ctgggttact cacctcgtta tattgaaaat 720
gatgatattc atgattacat ggcttatcat aaattagcag ctgaagtttt tgataaagcg 780
attgaagaca ttcatcaaat tcagaaagat gcgcgtgaag ataatcgtta tcaaaatgga 840
gagattccag cttggccaat tgttatcgca cgtttaccaa aaggttgggg tggtccacgt 900
tataatgatt ggtcaggtcc taaatttgac ggtaagggaa tgccaattga acatagtttc 960
cgtgcgcatc aagttccact tccgttatct tctaaaaata tgggaacttt accagaattt 1020
gtaaaatgga tgacttctta ccaaccagaa actttattta atgctgatgg aagtttgaaa 1080
gaagagttgc gtgattttgc accaaaaggt gagatgcgaa tggcttcaaa ccctgtaaca 1140
aatgggggag ttgattcttc taatttggtt ttaccagatt ggcaagaatt tgcaaatcca 1200
atttctgaaa ataatcgagg gaaattactc cctgatacaa atgacaatat ggatatgaat 1260
gttttgtcaa aatattttgc tgaaatagtc aaacttaatc ctacgcgttt ccgtttgttt 1320
ggtcctgatg aaaccatgtc taatcgtttt tgggaaatgt ttaaggtgac gaatcgtcag 1380
tggatgcaag tcataaaaaa tccaaatgat gaatttatct cacctgaggg tcgcattatt 1440
gattctcaat tatcagaaca ccaagcagaa ggttggcttg aaggttatac tttaaccgga 1500
cgcacaggag catttgcaag ttatgaatca ttcttgcgag tcgtagattc aatgttaact 1560
caacatttca aatggattcg tcaagctgca gaccaaaaat ggcgccatga ttatccttcg 1620
cttaatgtta tttcgacctc aaccgttttc caacaagacc ataatggtta tactcaccaa 1680
gatcctggaa tgttgactca tttggctgaa aagaaatctg attttatcag acaatacttg 1740
ccggctgatg ggaatacttt gcttgccgta tttgaccgtg cttttcaaga tagaagtaaa 1800
attaatcata ttgtagcctc taaacaacct cgtcaacaat ggtttactaa agaagaagct 1860
gaaaaattgg cgactgacgg aattgcaaca attgattggg cttcaacggc taaagatgga 1920
gaagcagtag atttagtttt tgcctcagca ggagctgagc ctacaattga aacactggca 1980
gctttacatc ttgtaaacga agttttccca caggcaaaat tccgttatgt gaacgtggtt 2040
gaattgggtc ggttgcaaaa gaaaaaggga gcactcaatc aagaacgtga actctcagat 2100
gaagaatttg aaaaatactt tggcccttca ggcactccag taatttttgg attccatgga 2160
tatgaagatt taatcgaatc cattttctat caaagaggac atgatggttt gattgttcat 2220
ggttaccgtg aagatggtga catcacgacg acttatgata tgcgggttta ctctgagctt 2280
gaccgtttcc accaagcgat tgatgccatg caagttctat atgtcaaccg aaaagttaat 2340
caaggtctag cgaaagcttt cattgaccga atgaaacgga cactagttaa acactttgaa 2400
gtgacaagaa atgaaggagt tgatattcct gattttactg aatgggtttg gtcggattta 2460
aagaaatag 2469
<210> 125
<211> 780
<212> PRT
<213> Aspergillus nidulans
<400> 125
Met Pro Gly Glu Val Ile Glu Arg Pro Asn Pro Ala Pro Lys Pro Ser
1 5 10 15
His Val Pro Asp Leu Val Glu Lys Leu Ile Ile Pro Ala Gln Lys Thr
20 25 30
Lys Leu Glu Lys Ser Asp Cys Asp Ala Leu His Lys Tyr Arg Arg Ala
35 40 45
Ala Ala Tyr Ile Ala Ala Gly His Trp Gly Thr Cys Pro Gly Leu Ile
50 55 60
Leu Val Tyr Ser His Leu Asn Tyr Leu Ile Lys Lys Gln Asn Leu Asp
65 70 75 80
Met Leu Tyr Val Val Gly Pro Gly His Gly Ala Pro Gly Leu Leu Ala
85 90 95
Ser Leu Trp Leu Glu Gly Ser Leu Gly Lys Phe Tyr Pro Gln Tyr Thr
100 105 110
Lys Asp Lys Glu Gly Leu His Asn Leu Ile Ser Thr Phe Ser Thr Ser
115 120 125
Ala Gly Leu Pro Ser His Ile Asn Ala Glu Thr Pro Gly Ala Ile His
130 135 140
Glu Gly Gly Glu Leu Gly Tyr Ala Leu Ser Val Ser Phe Gly Ala Val
145 150 155 160
Met Asp Asn Pro Asp Leu Ile Val Thr Cys Val Val Gly Asp Gly Glu
165 170 175
Ala Glu Thr Gly Pro Thr Ala Thr Ser Trp His Ala Ile Lys Tyr Ile
180 185 190
Asp Pro Ala Glu Ser Gly Ala Val Leu Pro Ile Leu His Val Asn Gly
195 200 205
Phe Lys Ile Ser Glu Arg Thr Ile Phe Gly Cys Met Asp Asn Arg Glu
210 215 220
Ile Val Cys Leu Phe Thr Gly Tyr Gly Tyr Gln Val Arg Ile Val Glu
225 230 235 240
Asp Leu Glu Asp Ile Asp Asn Asp Leu His Ser Ala Met Ser Trp Ala
245 250 255
Val Glu Glu Ile Arg Asn Ile Gln Lys Ala Ala Arg Ser Gly Lys Pro
260 265 270
Ile Met Lys Pro Gln Trp Pro Met Ile Val Leu Arg Thr Pro Lys Gly
275 280 285
Trp Ser Gly Pro Lys Glu Leu His Gly Gln Phe Ile Glu Gly Ser Phe
290 295 300
His Ser His Gln Val Pro Leu Pro Asn Ala Lys Lys Asp Asp Glu Glu
305 310 315 320
Leu Gln Ala Leu Gln Lys Trp Leu Ser Ser Tyr Lys Pro Asp Glu Leu
325 330 335
Phe Thr Glu Ser Gly Asp Val Ile Asp Glu Ile Leu Ser Ile Ile Pro
340 345 350
Ser Asp Asp Lys Lys Leu Gly Met Arg Pro Glu Ala Tyr Lys Thr His
355 360 365
Leu Pro Pro Asp Leu Pro Asp Trp Arg Gln Phe Cys Val Lys Lys Gly
370 375 380
Asp Gln Phe Ser Ala Met Lys Ala Ile Gly Ser Phe Ile Asp Gln Val
385 390 395 400
Phe Val Lys Asn Pro His Thr Val Arg Leu Phe Ser Pro Asp Glu Leu
405 410 415
Glu Ser Asn Lys Leu Ser Ala Ala Leu Ser His Thr Gly Arg Asn Phe
420 425 430
Gln Trp Asp Glu Phe Ser Asn Ala Lys Gly Gly Arg Val Ile Glu Val
435 440 445
Leu Ser Glu His Leu Cys Gln Gly Phe Met Gln Gly Tyr Thr Leu Thr
450 455 460
Gly Arg Thr Gly Ile Phe Pro Ser Tyr Glu Ser Phe Leu Gly Ile Ile
465 470 475 480
His Thr Met Met Val Gln Tyr Ala Lys Phe Ala Lys Met Ala Lys Glu
485 490 495
Thr Ala Trp His His Asp Val Ser Ser Ile Asn Tyr Ile Glu Thr Ser
500 505 510
Thr Trp Ala Arg Gln Glu His Asn Gly Phe Ser His Gln Asn Pro Ser
515 520 525
Phe Ile Gly Ala Val Leu Lys Leu Lys Pro Tyr Ala Ala Arg Val Tyr
530 535 540
Leu Pro Pro Asp Ala Asn Thr Phe Leu Thr Thr Leu His His Cys Leu
545 550 555 560
Lys Ser Lys Asn Tyr Ile Asn Leu Met Val Gly Ser Lys Gln Pro Thr
565 570 575
Pro Val Tyr Leu Ser Pro Glu Glu Ala Glu Ser His Cys Arg Ala Gly
580 585 590
Ala Ser Ile Phe Lys Phe Cys Ser Thr Asp Gly Gly Leu Arg Pro Asp
595 600 605
Val Val Leu Val Gly Ile Gly Val Glu Val Met Phe Glu Val Ile Lys
610 615 620
Ala Ala Ala Ile Leu Arg Glu Arg Cys Pro Glu Leu Arg Val Arg Val
625 630 635 640
Val Asn Val Thr Asp Leu Phe Ile Leu Glu Asn Glu Gly Ala His Pro
645 650 655
His Ala Leu Lys His Glu Ala Phe Asp Asn Leu Phe Thr Glu Asp Arg
660 665 670
Ser Ile His Phe Asn Tyr His Gly Tyr Val Asn Glu Leu Gln Gly Leu
675 680 685
Leu Phe Gly Arg Pro Arg Leu Asp Arg Ala Thr Ile Lys Gly Tyr Lys
690 695 700
Glu Glu Gly Ser Thr Thr Thr Pro Phe Asp Met Met Leu Val Asn Glu
705 710 715 720
Val Ser Arg Tyr His Val Ala Lys Ala Ala Val Thr Gly Gly Ala Arg
725 730 735
Phe Asn Glu Lys Val Lys Leu Arg His Gln Glu Leu Cys Ser Glu Phe
740 745 750
Asp His Asn Ile Ala Glu Thr Arg Lys Tyr Ile Met Asn Asn His Gln
755 760 765
Asp Pro Glu Asp Thr Tyr Asn Met Pro Ser Phe Asn
770 775 780
<210> 126
<211> 2343
<212> DNA
<213> Aspergillus nidulans
<400> 126
atgccaggag aagtcatcga gcgaccaaac ccggcgccaa agccgtcgca tgtccccgat 60
ttagtagaaa aactcataat tcccgcgcag aagacgaagc tggagaaaag cgattgtgat 120
gcgttgcaca agtatcgtcg cgcggcggcg tatattgctg ctggacactg gggaacatgt 180
cctgggttga tcctcgttta ctctcacctg aactacctga tcaagaagca gaacctcgat 240
atgctgtatg ttgttgggcc agggcatgga gcaccgggct tgctagcatc gttgtggctt 300
gagggctcgc tggggaagtt ttatccgcag tataccaagg acaaggaggg tctgcacaac 360
ctcatctcga cgttcagtac cagtgccgga ctgcctagcc acatcaatgc tgaaacaccc 420
ggcgctatcc acgaaggagg agagctgggc tatgcgctgt ctgtctcctt cggcgcagtc 480
atggataacc ccgatttgat tgtaacatgt gtagttggag acggagaggc ggaaactggt 540
ccaacagcca catcatggca tgcgatcaag tacatcgatc cagctgagtc aggagccgtg 600
cttccaatcc tgcatgtgaa tggcttcaag atcagtgagc gcactatttt tgggtgcatg 660
gataacaggg agatagtctg cctgttcaca gggtacgggt atcaggtgcg cattgtcgag 720
gacctcgagg acatcgacaa cgaccttcac agcgctatgt cctgggcggt tgaggaaatc 780
cgtaatattc agaaagcagc gcgctccgga aagcctatca tgaagcctca atggcccatg 840
attgtcttgc gaacgcccaa gggttggtca gggccgaaag agctgcatgg ccagttcatc 900
gaaggatcgt tccactccca ccaggttccc ctccctaatg ctaagaaaga cgatgaggag 960
ctccaggctc tgcagaaatg gctttcctct tacaaacccg atgagctgtt taccgagtct 1020
ggcgacgtta tcgacgaaat cctatccatt attccttcgg atgataagaa actcggcatg 1080
agacccgagg cctacaagac tcatctaccg ccggacctcc ctgactggag acagttctgc 1140
gtgaaaaaag gggatcagtt cagcgcaatg aaggccattg gtagcttcat cgaccaggtt 1200
ttcgtcaaga acccgcatac cgtccggtta ttctcacccg acgagctgga aagcaacaag 1260
ttgagcgctg ccctatcaca tacgggaagg aatttccagt gggatgagtt ctcgaatgca 1320
aaaggtgggc gggtgatcga ggtcctgagt gagcatttgt gtcagggctt catgcagggg 1380
tatacattga ccggccggac gggcatcttc ccatcatatg agagtttctt gggtattata 1440
cataccatga tggtccagta tgccaagttc gcaaagatgg ctaaagaaac ggcatggcac 1500
catgacgtga gtagtatcaa ctacatcgag accagcacct gggcccgaca ggagcacaat 1560
ggcttctctc accaaaatcc atccttcatc ggcgcggttc tcaaactgaa gccgtacgcc 1620
gcccgcgtct acctgcctcc cgacgccaac acatttctta ccactttgca ccactgcctg 1680
aaatcaaaga attatatcaa cctcatggtc ggctcaaagc aacccacccc agtctacctg 1740
agccccgagg aagcggaaag ccactgccga gccggagcct cgatcttcaa gttctgcagt 1800
accgacggtg ggctccgccc ggatgtcgta ctcgttggaa tcggtgttga ggtcatgttc 1860
gaagttatca aggcggcagc catactgcga gaacgatgcc ctgagctgcg tgttcgtgta 1920
gtcaacgtga cggatttatt cattctcgag aacgagggtg cccaccccca cgccttgaag 1980
cacgaggcct tcgacaacct cttcaccgag gatcgctcca tccatttcaa ctatcatgga 2040
tatgtgaacg aactccaggg cctgctcttt ggccgcccta ggctcgaccg ggcaaccatc 2100
aagggatata aggaagaggg aagcaccaca actccatttg acatgatgct tgtgaatgaa 2160
gtatcgcggt accacgtcgc gaaggcagcc gtcacgggag gagcgaggtt caatgagaaa 2220
gtcaagctgc ggcaccagga gctttgctct gaattcgatc ataacattgc tgagacgcgc 2280
aagtacatca tgaacaatca tcaagatccc gaagacacat acaatatgcc ctcatttaac 2340
tag 2343
<210> 127
<211> 408
<212> PRT
<213> Methanosarcina acetivorans
<400> 127
Met Lys Val Leu Val Ile Asn Ala Gly Ser Ser Ser Leu Lys Tyr Gln
1 5 10 15
Leu Ile Asp Met Thr Asn Glu Ser Pro Leu Ala Ile Gly Leu Cys Glu
20 25 30
Arg Ile Gly Ile Asp Asn Ser Ile Ile Thr Gln Lys Arg Ser Asp Gly
35 40 45
Lys Lys Leu Glu Lys Gln Thr Asp Leu Pro Asn His Lys Val Ala Leu
50 55 60
Glu Glu Val Val Lys Ala Leu Thr Asp Ser Glu Leu Gly Val Ile Lys
65 70 75 80
Ser Met Asp Glu Ile Asn Ala Val Gly His Arg Val Val His Gly Gly
85 90 95
Glu Lys Phe Thr Ser Ser Ala Leu Ile Asp Glu Gly Val Glu Gln Ala
100 105 110
Ile Lys Asp Cys Phe Glu Leu Ala Pro Leu His Asn Pro Pro Asn Met
115 120 125
Met Gly Ile Thr Ala Cys Gln Glu Ile Met Pro Gly Val Pro Met Val
130 135 140
Ala Val Phe Asp Thr Ala Phe His Gln Thr Ile Pro Ala Tyr Ala Tyr
145 150 155 160
Met Tyr Ala Leu Pro Tyr Thr Leu Tyr Glu Lys Tyr Gly Ile Arg Lys
165 170 175
Tyr Gly Phe His Gly Thr Ser His Phe Tyr Val Ala Arg Arg Ala Ala
180 185 190
Ala Met Leu Gly Lys Pro Glu Glu Glu Val Lys Val Ile Thr Cys His
195 200 205
Leu Gly Asn Gly Ser Ser Ile Thr Ala Val Asn Gly Gly Lys Ser Val
210 215 220
Glu Thr Thr Met Gly Phe Thr Pro Leu Glu Gly Val Ala Met Gly Thr
225 230 235 240
Arg Cys Gly Ser Ile Asp Pro Ala Val Val Pro Phe Val Met Glu Lys
245 250 255
Glu Gly Leu Thr Thr Arg Glu Ile Asp Thr Leu Met Asn Lys Lys Ser
260 265 270
Gly Val Leu Gly Val Ser Gly Leu Ser Asn Asp Phe Arg Asp Leu Asp
275 280 285
Glu Ala Ala Ser Lys Gly Asn Gln Arg Ala Glu Leu Ala Leu Glu Ile
290 295 300
Phe Ala Tyr Lys Ile Lys Lys Val Ile Gly Glu Tyr Ser Ala Val Leu
305 310 315 320
Asn Gly Ala Asp Ala Val Val Phe Thr Ala Gly Ile Gly Glu Asn Ser
325 330 335
Ala Ser Ile Arg Lys Arg Ile Leu Ser Gly Leu Asp Gly Leu Gly Ile
340 345 350
Glu Ile Asp Glu Glu Lys Asn Lys Ile Arg Gly Gln Glu Ile Asp Ile
355 360 365
Ser Thr Pro Asp Ala Lys Val Arg Val Leu Val Ile Pro Thr Asn Glu
370 375 380
Glu Leu Thr Ile Ala Arg Asp Thr Lys Glu Ile Cys Glu Thr Glu Val
385 390 395 400
Lys Leu Arg Arg Ser Val Ser Ile
405
<210> 128
<211> 1227
<212> DNA
<213> Methanosarcina acetivorans
<400> 128
atgaaagtac tggttataaa cgcagggagt tcttctctca aatatcaatt aattgatatg 60
acaaacgagt cccctcttgc aatagggctc tgtgagagga taggcatcga caactcgatc 120
attacccaga agaggtctga cggcaagaaa ctggaaaagc agaccgacct tcctaaccac 180
aaggttgccc ttgaggaagt cgtcaaggct cttacggact ccgagctcgg tgtcatcaaa 240
agcatggacg aaatcaatgc agtcgggcac agagttgtgc acggtggaga aaagttcact 300
tcctcggctt tgatcgatga aggcgtagaa caggcaatca aggactgctt tgaactggct 360
cctctccaca atcctccaaa catgatggga attactgcct gtcaggagat catgccaggc 420
gtcccgatgg ttgctgtttt tgacacagca ttccaccaga caatcccggc ctatgcctac 480
atgtatgctc tgccgtacac actgtacgaa aagtacggga tcagaaaata cggtttccac 540
ggcacttctc acttttacgt tgccagaagg gctgccgcta tgctcggaaa acccgaagaa 600
gaagtcaaag tcatcacctg ccacctagga aacggctcaa gcattacggc tgttaacggc 660
ggaaaatccg ttgagacaac aatgggcttt accccgctcg aaggagttgc aatgggtacc 720
aggtgcggtt cgattgaccc tgcagtagtc cccttcgtta tggaaaagga aggccttacg 780
acccgagaaa tcgataccct catgaacaag aagtcaggcg tgcttggagt ctccgggctc 840
agcaacgact tcagggacct cgatgaagca gcttccaaag gcaaccagag agccgaactc 900
gcccttgaaa tctttgcata caagattaag aaggtcatag gtgagtattc agccgtactc 960
aatggtgcgg atgcggtagt ctttacagca ggcattggag aaaacagtgc aagtatcagg 1020
aagagaatcc tctccggcct tgacggtctc ggcatagaga tcgacgaaga aaagaacaag 1080
atcagaggtc aggaaatcga tatcagcact cctgatgcaa aagtaagagt ccttgttatc 1140
ccgaccaacg aagaactaac cattgcaagg gacacaaagg aaatctgtga gaccgaagta 1200
aagctgcgca gatcagtatc aatctga 1227
<210> 129
<211> 408
<212> PRT
<213> Methanosarcina barkeri
<400> 129
Met Lys Val Leu Val Ile Asn Ala Gly Ser Ser Ser Leu Lys Tyr Gln
1 5 10 15
Leu Ile Asp Met Ile Asn Glu Ser Pro Leu Ala Val Gly Leu Cys Glu
20 25 30
Arg Val Gly Ile Asp Asn Ser Ile Ile Thr Gln Lys Arg Phe Asp Gly
35 40 45
Lys Lys Leu Glu Lys Gln Val Asp Leu Pro Thr His Arg Val Ala Leu
50 55 60
Glu Glu Val Val Lys Ala Leu Thr Asp Pro Glu Phe Gly Val Ile Thr
65 70 75 80
Asp Met Gly Glu Ile Asn Ala Val Gly His Arg Val Val His Gly Gly
85 90 95
Glu Lys Phe Thr Thr Ser Ala Leu Phe Asp Ala Gly Val Glu Glu Ala
100 105 110
Ile Arg Asp Cys Phe Asp Leu Ala Pro Leu His Asn Pro Pro Asn Met
115 120 125
Met Gly Ile Ser Ala Cys Ala Glu Ile Met Pro Gly Thr Pro Met Val
130 135 140
Ile Val Phe Asp Thr Ala Phe His Gln Thr Met Pro Ala Tyr Ala Tyr
145 150 155 160
Met Tyr Ala Leu Pro Tyr Asp Leu Tyr Glu Lys Tyr Gly Val Arg Lys
165 170 175
Tyr Gly Phe His Gly Thr Ser His Lys Tyr Val Ala Gly Arg Ala Ala
180 185 190
Leu Met Leu Gly Lys Pro Ile Glu Asp Thr Lys Ile Ile Thr Cys His
195 200 205
Leu Gly Asn Gly Ser Ser Ile Ala Ala Val Lys Gly Gly Lys Ser Ile
210 215 220
Asp Thr Ser Met Gly Phe Thr Pro Leu Glu Gly Val Ala Met Gly Thr
225 230 235 240
Arg Cys Gly Ser Ile Asp Pro Ala Val Val Pro Phe Val Met Asp Lys
245 250 255
Glu Ser Leu Ser Ser Arg Glu Val Asp Thr Leu Met Asn Lys Lys Ser
260 265 270
Gly Val Leu Gly Val Ser Gly Ile Ser Asn Asp Phe Arg Asp Leu Asp
275 280 285
Glu Ala Ala Ser His Gly Asn Glu Arg Ala Glu Leu Ala Leu Glu Ile
290 295 300
Phe Ala Tyr Ser Val Lys Arg Val Ile Gly Glu Tyr Leu Ala Val Leu
305 310 315 320
Asn Gly Ala Asp Ala Ile Val Phe Thr Ala Gly Ile Gly Glu Asn Ser
325 330 335
Ala Ser Ile Arg Lys Arg Ile Leu Thr Gly Leu Glu Gly Leu Gly Ile
340 345 350
Lys Ile Asp Glu Glu Lys Asn Lys Ile Arg Gly Gln Glu Ile Asp Ile
355 360 365
Ser Thr Pro Asp Ser Ser Ile Arg Val Phe Val Ile Pro Thr Asn Glu
370 375 380
Glu Leu Ala Ile Ala Arg Glu Thr Lys Glu Ile Val Glu Thr Glu Ala
385 390 395 400
Lys Leu Arg Lys Ser Val Pro Val
405
<210> 130
<211> 1227
<212> DNA
<213> Methanosarcina barkeri
<400> 130
atgaaggtat tggtaataaa tgcaggaagc tcatcgctga aatatcagtt aattgatatg 60
atcaatgaat cccctcttgc agtcggtctt tgcgaaaggg tgggaatcga taactcgatc 120
attactcaga agaggttcga tggcaagaag ctggaaaagc aggttgacct gccaacccac 180
agagtagccc ttgaagaagt tgtcaaggct cttacggatc cggaatttgg tgtcatcaca 240
gacatgggtg aaatcaacgc ggtcggacac agggttgtgc acggtggcga gaagttcaca 300
acatctgctt tatttgatgc aggtgtcgag gaagctatta gagattgctt cgatctggct 360
cctctccaca accctccaaa catgatggga atttcagcct gtgcagagat catgcctgga 420
acgcccatgg ttattgtttt tgacactgca ttccatcaga ccatgcctgc gtatgcctac 480
atgtatgctt tgccatatga cctctacgaa aagtatggag tgcgaaaata cggtttccac 540
gggacttccc acaagtatgt cgctggaaga gccgctctta tgcttggaaa gcctatagaa 600
gatacaaaga ttatcacctg tcacctcgga aatggttcaa gtattgcagc agtgaaaggt 660
ggaaaatcta ttgataccag catgggcttt actcctcttg aaggggttgc aatgggtacc 720
agatgcggtt ccattgatcc ggcagtcgtt cccttcgtta tggataaaga aagcctgtca 780
agcagagagg ttgatactct catgaacaag aagtctggcg tacttggagt ttccgggata 840
agcaacgact tcagagacct tgatgaggct gcctctcatg gaaacgagag agctgagctt 900
gcccttgaga tcttcgcata tagtgtcaag agagtcattg gtgagtattt agccgtgctc 960
aatggtgcag atgcgattgt ctttaccgca ggtatcggag aaaacagcgc aagcatcaga 1020
aagagaatcc ttactggtct tgaaggtctc ggtataaaaa tcgatgagga aaagaacaag 1080
atcagaggcc aggaaatcga catcagtacc cctgattcga gtataagggt ttttgtcatt 1140
ccaaccaatg aagaacttgc cattgcaagg gaaacaaagg aaattgttga gaccgaagct 1200
aaactacgta aatcggtacc tgtttga 1227
<210> 131
<211> 346
<212> PRT
<213> Gluconacetobacter diazotrophicus
<400> 131
Met Leu Val Asp Arg Thr Trp Pro Arg Pro Asp Thr Gly Ala Asp Pro
1 5 10 15
His Gln Gly Pro Val Gly Ser Leu Ile Asp Trp Val Ala Ser His Leu
20 25 30
Gly Asp Val Pro Leu Val Gly Val Gly His Arg Val Val His Gly Gly
35 40 45
Pro Asp Phe Ile Ala Pro Val Arg Ile Thr Pro Asp Val Leu Ala Arg
50 55 60
Leu Asp Ala Leu Thr Pro Phe Ala Pro Leu His Gln Pro Ala Ser Leu
65 70 75 80
Gly Pro Ile Arg Ala Leu Thr Ala Leu His Pro Asp Leu Pro Gln Val
85 90 95
Ala Cys Phe Asp Thr Ala Phe His His Thr Met Pro Ala Thr Ala Thr
100 105 110
Arg Leu Ala Leu Pro Asp Ala Tyr Gly Arg Lys Gly Val Arg Arg Tyr
115 120 125
Gly Phe His Gly Leu Ser Tyr Glu Tyr Ile Ala Ser Cys Leu Pro Gly
130 135 140
Leu Ser Pro Arg Leu Ala Ala Gly Arg Thr Leu Val Ala His Leu Gly
145 150 155 160
Asn Gly Ala Ser Leu Cys Ala Met Gln Ala Gly Arg Ser Ile Glu Thr
165 170 175
Thr Met Gly Phe Ser Val Leu Asp Gly Leu Val Met Gly Thr Arg Cys
180 185 190
Gly Gln Leu Asp Pro Gly Val Ile Leu Tyr Met Leu Arg Ala Glu Lys
195 200 205
Leu Asp Val Ala Gly Ile Glu Asp Val Leu Tyr Arg Gln Ser Gly Leu
210 215 220
Leu Gly Leu Ser Gly Val Ser Ser Asp Met Arg Asp Leu Gln Glu Arg
225 230 235 240
Ala Ala Gly Asn Asp Gly Ala Arg Gln Ala Leu Glu Met Phe Thr Tyr
245 250 255
Arg Leu Val Gln Gln Ala Gly Ser Met Val Ala Val Leu Gly Gly Leu
260 265 270
Asp Gly Leu Val Phe Thr Ala Gly Ile Gly Glu His Asp Ala Pro Ile
275 280 285
Arg Ala Ala Ala Cys Ala Arg Leu Ser Trp Leu Gly Leu Arg Leu Asp
290 295 300
Ala Ala Ala Asn Ala Ala His Ala Pro Val Ile Ser Thr Pro Asp Ser
305 310 315 320
Ala Val Glu Val Arg Val Ile Pro Thr Asp Glu Glu Ser Met Ile Arg
325 330 335
Arg His Val Ala Asp Cys Leu Ala Gly Glu
340 345
<210> 132
<211> 1041
<212> DNA
<213> Gluconacetobacter diazotrophicus
<400> 132
atgctggtcg accggacatg gcccaggccc gacacagggg ccgatcccca tcagggcccg 60
gtcggcagcc tgattgactg ggtggcctcg cacctggggg acgttccgct ggtcggggta 120
gggcaccggg tggtgcatgg cggtcccgac ttcatcgccc cggtgcggat cacgcccgat 180
gtcctggcgc ggctggacgc gctgacaccg ttcgcgccgt tgcaccagcc ggccagcctg 240
gggccgatcc gcgccctgac ggccctgcat cccgacctgc cgcaggtcgc ctgcttcgat 300
accgccttcc accacaccat gcccgccacg gccacgcggc tggcgctgcc ggacgcctat 360
gggcgcaagg gggtgcggcg gtatgggttc cacggcctgt cctacgaata tatcgcctcc 420
tgcctgcccg gcctgtcgcc ccggctggcg gccgggcgca cgctggtggc gcatctgggc 480
aatggggcca gcctgtgcgc gatgcaggcg gggcgcagca tcgagaccac gatggggttc 540
tcggtactgg acgggctggt gatgggcacg cgctgcggcc agctcgatcc cggcgtcatc 600
ctgtacatgc tgcgcgcgga aaaactggac gtggcgggga tcgaggacgt gctgtaccgc 660
cagtcgggcc tgctgggcct gtcgggcgtt tccagcgaca tgcgcgacct gcaggaacgc 720
gcggccggga atgacggcgc ccgccaggcg ctggagatgt tcacataccg cctggtccag 780
caggcgggtt cgatggtcgc ggtgctgggt ggactggacg gactggtctt taccgccggc 840
atcggcgagc atgacgcccc gatccgggcg gcggcgtgcg cgcgcctgtc ctggctgggg 900
ctccgcctgg acgctgccgc caacgcggcc cacgccccgg tgatcagcac gccggacagc 960
gcggtggagg tccgcgtcat tcccacggac gaggaaagca tgatccgccg ccacgtcgcg 1020
gattgcctgg cgggggaatg a 1041
<210> 133
<211> 417
<212> PRT
<213> Aspergillus niger
<400> 133
Met Ala Arg Lys Ser Ile Leu Ser Val Asn Ala Gly Ser Ser Ser Val
1 5 10 15
Lys Leu Thr Phe Tyr Thr Tyr Glu Lys Thr Pro Lys Val Ile Ala Ala
20 25 30
Ala Gln Val Ser Gly Ile Thr Ala Pro Pro Ala Lys Leu Lys Tyr Thr
35 40 45
Ser Gly Asp Lys Gln His Lys Glu Glu Leu Lys Glu Ser Ile Ser Thr
50 55 60
Pro Gln Asp Ala Phe Lys Phe Leu Leu Gln Arg Cys Ile Ser Asp Pro
65 70 75 80
Glu Leu Ser Glu Val Ala Ser Thr Asp Asp Leu Ala Tyr Ile Cys His
85 90 95
Arg Val Val His Gly Gly Asp Tyr Glu Thr Ser Val Val Ile Thr Asp
100 105 110
Glu Thr Tyr His His Leu Glu Lys Leu Glu Asp Leu Ala Pro Leu His
115 120 125
Asn Tyr Ser Ala Leu Glu Ile Ile Arg His Cys Arg Lys Glu Ile Pro
130 135 140
Ser Val Arg Ser Ile Thr Phe Phe Asp Ser Ala Phe His Gln Thr Leu
145 150 155 160
Pro Glu His Val Lys Thr Tyr Pro Ile Asp Gln Lys Ile Ala Lys Ser
165 170 175
Asn Gly Leu Arg Lys Tyr Gly Phe His Gly Ile Ser Tyr Ser Phe Ile
180 185 190
Leu Arg Ser Val Ala Glu Val Leu Asn Lys Pro Ala Asp Lys Thr Asn
195 200 205
Ile Ile Ala Met His Ile Gly Ser Gly Ala Ser Ile Cys Ala Ile Lys
210 215 220
Asp Gly Lys Ser Val Asp Thr Thr Met Gly Leu Thr Pro Leu Ala Gly
225 230 235 240
Leu Pro Gly Ala Thr Arg Ser Gly Asp Ile Asp Pro Ser Leu Val Phe
245 250 255
His Tyr Thr Asn Asp Ala Gly Lys Leu Ser Pro Ala Ser Thr Lys Glu
260 265 270
Met His Ile Ser Thr Ala Glu Glu Ile Leu Asn Lys Lys Ser Gly Trp
275 280 285
Lys Ala Leu Thr Gly Thr Thr Asp Phe Ala Gln Ile Ala Val Glu Asn
290 295 300
Pro Pro Thr Arg Glu His Lys Leu Ala Phe Asp Ile Leu Val Asp Arg
305 310 315 320
Ile Ala Gly Tyr Leu Gly Asn Tyr Phe Val Lys Leu Asp Gly His Val
325 330 335
Asp Ala Phe Val Phe Ala Gly Gly Ile Gly Glu Lys Ser Ala Leu Leu
340 345 350
Arg Lys Ala Val Thr Glu Lys Cys Arg Cys Leu Gly Cys Ala Val Asp
355 360 365
Pro Gly Lys Asn Asp Lys Gly Ala Gly Asp Gly Glu Thr Val Val Asp
370 375 380
Ile Ser Arg Gly Asp Asp Lys Gly Pro Lys Val Leu Ile Cys Gln Thr
385 390 395 400
Asp Glu Gln Phe Glu Met Ala Tyr Gly Cys Val Asn Gln Tyr Glu Arg
405 410 415
Pro
<210> 134
<211> 1254
<212> DNA
<213> Aspergillus niger
<400> 134
atggcgcgca aatccatcct ctccgtcaat gcgggatcct cttcggtcaa gcttaccttc 60
tatacctacg agaaaacccc caaggtcatt gctgcagctc aggtctctgg tatcaccgcc 120
ccgccagcaa agctgaaata caccagtggc gacaagcaac acaaagagga actcaaggaa 180
agcatcagta ctcctcaaga tgccttcaaa ttcctgctcc agcgctgcat ctctgatccc 240
gagctttccg aagtcgccag cacagacgat ctagcctata tctgccaccg tgtggtgcac 300
ggtggagact acgaaacgtc tgttgtaatc actgacgaaa cctatcatca ccttgaaaaa 360
ttggaagacc tagctccatt acacaattac tccgccttgg aaattatccg gcattgcagg 420
aaggagattc catccgtcag gagcatcacc ttctttgact cggcgttcca tcaaaccctc 480
cccgaacacg tcaagaccta ccccatcgac cagaagatag ccaagtccaa cgggcttcgg 540
aaatatggct tccatggaat cagctattcg ttcattctac gctccgtagc cgaggtcctg 600
aacaaacctg ctgacaagac caatatcatt gcaatgcata tcggaagcgg tgcttctatt 660
tgcgccatca aagatggcaa atcagttgat actacgatgg gactcacgcc cctagccgga 720
ctaccaggcg ccacgcgaag cggtgacatt gatccatccc tcgtgttcca ctacaccaac 780
gatgcaggca aacttagccc cgccagcacc aaagaaatgc acatcagcac ggccgaagaa 840
atcctgaaca agaagtccgg ctggaaagcc ttgactggca caaccgactt tgctcaaatc 900
gccgtcgaga acccgccgac acgggaacat aaactggcat tcgacatcct cgttgaccgc 960
atcgccggct acctcggcaa ttactttgtg aaactagacg gccacgtcga cgccttcgtc 1020
ttcgcaggcg gaataggcga gaaaagtgcg ctgcttcgca aagcagttac ggagaagtgt 1080
cgctgcctgg gttgtgcggt cgacccaggg aagaatgaca agggcgcggg ggatggggag 1140
acggtagtgg atatttcgag gggtgatgac aagggcccta aggtgttgat ctgtcagaca 1200
gatgaacagt tcgagatggc atacggatgc gtcaatcagt atgagaggcc gtag 1254
<210> 135
<211> 437
<212> PRT
<213> Penicillium chrysogenum
<400> 135
Met Pro Lys Ser Ile Leu Ala Val Asn Ala Gly Ser Ser Ser Val Lys
1 5 10 15
Ile Thr Phe Tyr Thr Phe Asp Asn Pro Pro Arg Thr Ile Val Asp Ala
20 25 30
Ala Ile Ser Gly Ile Thr Ala Pro Pro Ser Thr Leu Lys Tyr Gln Ala
35 40 45
Gly Gly Arg Lys His Lys Glu Glu Leu Lys Glu Lys Leu Ser Thr Ala
50 55 60
Gln Asp Ala Phe Lys Tyr Leu Leu Gln Arg Cys Phe Ser Asp Pro Glu
65 70 75 80
Leu Ser Glu Val Ala Ser Ala Asp Asp Val Glu Tyr Ile Cys His Arg
85 90 95
Val Val His Gly Gly Asp Tyr Arg Asp Ala Val Glu Ile Asn Asp Glu
100 105 110
Thr Leu Gly His Leu Lys Gly Leu Glu Asp Leu Ala Pro Leu His Asn
115 120 125
Phe Ser Ala Leu Glu Ile Val Arg Leu Cys Arg Ser Glu Leu Pro Lys
130 135 140
Val Arg Ser Ile Thr Phe Phe Asp Ser Ser Phe His Gln Thr Ile Pro
145 150 155 160
Glu Ala Val Arg Thr Tyr Pro Ile Asn Gln Glu Ile Ala Lys Ala Asn
165 170 175
Gly Leu Arg Lys Tyr Gly Phe His Gly Ile Ser Tyr Ser Phe Ile Leu
180 185 190
Arg Ser Val Ala Gln Phe Leu Asn Lys Pro Val Glu Lys Thr Asn Leu
195 200 205
Ile Val Met His Ile Gly Ser Gly Ala Ser Ile Cys Ala Ile Lys Asp
210 215 220
Gly Lys Ser Val Asp Thr Ser Met Gly Leu Thr Pro Leu Ala Gly Leu
225 230 235 240
Pro Gly Ala Thr Arg Ser Gly Ser Ile Asp Pro Ser Leu Val Phe His
245 250 255
Tyr Thr Asn Glu Ala Gly Lys Leu Ser Pro Ala Ser Thr Ser Glu Met
260 265 270
His Ile Ser Thr Ala Glu Asp Ile Leu Asn Lys Gln Ser Gly Trp Lys
275 280 285
Ala Leu Thr Gly Thr Thr Asp Phe Ala Gln Ile Ala Val Pro Asn Pro
290 295 300
Pro Ser Glu Ala His Lys Leu Ala Phe Asp Ile Phe Val Asp Arg Ile
305 310 315 320
Gln Gly Tyr Ile Gly Ser Tyr Tyr Val Lys Leu Asn Gly Glu Leu Asp
325 330 335
Gly Val Val Phe Ala Gly Gly Ile Gly Glu Lys Ser Ala Leu Leu Arg
340 345 350
Arg Thr Leu Val Asp Lys Cys Gln Cys Leu Gly Leu Ala Ile Asp Asp
355 360 365
Val Ala Asn Asp Lys Gly Pro Gly Asp Glu Glu Thr Val Lys Asp Ile
370 375 380
Ser Lys Gly Ser Gly Lys Gly Pro Arg Val Leu Ile Cys Gln Thr Asn
385 390 395 400
Glu Gln Val Cys Gly Leu Lys Tyr Trp Val Leu Leu Thr Val Thr Phe
405 410 415
Ala Leu Thr Ser Arg Gly Gly Val Gly Phe Gly Tyr Arg Leu Phe Leu
420 425 430
Tyr Thr Trp Ile Tyr
435
<210> 136
<211> 1314
<212> DNA
<213> Penicillium chrysogenum
<400> 136
atgcccaaat ctatcctcgc tgttaacgcg ggttcgtctt ccgtcaaaat caccttctat 60
accttcgaca acccgccaag aaccattgtg gatgcggcga tctctggaat cacagcaccc 120
ccgtctacac tcaagtacca agcaggaggc agaaagcaca aggaggagct caaagaaaaa 180
ctcagtaccg cccaagatgc attcaagtac cttctacagc gctgtttcag cgaccccgaa 240
ctttccgaag tcgccagcgc cgacgatgtg gagtatatct gccaccgagt cgtccacggt 300
ggagactacc gcgatgcagt cgaaatcaac gacgagacac ttggccacct gaaaggattg 360
gaagatcttg cacctctcca taatttctcc gcgctggaga tagtccgcct gtgcagatca 420
gagctcccca aagtcagaag cataactttt ttcgactctt ctttccacca gactatcccc 480
gaagccgtac gtacataccc aatcaaccag gaaatcgcca aggcgaatgg tctgcgcaag 540
tatggatttc acgggatcag ctactccttc atcctgcggt ccgtagcaca gtttttgaac 600
aaaccggtcg agaagacgaa tctcatcgtg atgcatatcg ggagcggagc ctcgatctgt 660
gcgatcaagg acgggaagtc cgtggatact tcaatgggtc tcactccttt ggcaggatta 720
cccggtgcaa cgcgtagtgg aagcattgat ccatcattgg tcttccacta taccaacgaa 780
gcaggcaaac taagcccagc cagcacgtct gaaatgcaca tcagcacggc ggaggatatc 840
ctcaataaac aatcaggttg gaaagccctc actggaacaa ccgacttcgc ccagattgcg 900
gtgccgaacc caccaagcga agcacataaa ctcgcttttg acattttcgt cgaccgcatc 960
cagggctaca tcggcagcta ctacgtcaaa ctaaatggtg aattagacgg tgtagtcttc 1020
gccggcggga tcggtgagaa gagtgccttg ctgagacgaa cactggtcga taaatgccag 1080
tgtctaggtt tggcaatcga tgatgtggct aatgacaagg ggcctggaga tgaggagacg 1140
gtcaaggata tctccaaggg ttccggcaag gggccccggg ttttaatttg ccagaccaat 1200
gaacaggtat gtggtctcaa atattgggta ttattgaccg tgaccttcgc cttgacctct 1260
cgtggaggtg tagggtttgg ttataggtta tttttgtaca cttggattta ctga 1314
<210> 137
<211> 401
<212> PRT
<213> Clostridium acetobutylicum
<400> 137
Met Lys Asn Leu Val Ile Asn Cys Gly Ser Ser Ser Ile Lys Tyr Gln
1 5 10 15
Phe Ile Asp Met Lys Asp Glu Thr Val Leu Ala Lys Gly Leu Val Glu
20 25 30
Arg Ile Gly Ile Lys Gly Ser Val Ile Thr His Lys Val Asn Gly Glu
35 40 45
Lys Tyr Val Thr Glu Thr Pro Met Glu Asp His Lys Lys Ala Ile Lys
50 55 60
Leu Val Leu Asp Ala Leu Leu Asn Asp Glu Tyr Gly Val Ile Lys Asn
65 70 75 80
Ile Asp Glu Ile Ser Ala Val Gly His Arg Ile Val His Gly Gly Glu
85 90 95
Lys Tyr Ala Asn Ser Val Leu Ile Asp Glu Asp Val Met Lys Ser Ile
100 105 110
Glu Asp Cys Val Ser Leu Ala Pro Leu His Asn Pro Pro His Ile Ile
115 120 125
Gly Ile Asn Ala Cys Lys Glu Leu Met Pro Asn Val Pro Met Val Ala
130 135 140
Val Phe Asp Thr Ala Phe His Gln Thr Ile Pro Asp Tyr Ala Tyr Met
145 150 155 160
Tyr Ala Ile Pro Tyr Glu Tyr Tyr Asp Lys Tyr Lys Ile Arg Lys Tyr
165 170 175
Gly Phe His Gly Thr Ser His Lys Tyr Val Ser Arg Thr Ala Ala Glu
180 185 190
Phe Ile Gly Lys Lys Val Glu Asp Leu Lys Met Val Val Cys His Met
195 200 205
Gly Asn Gly Ala Ser Ile Thr Ala Val Glu Asn Gly Lys Ser Val Asp
210 215 220
Thr Ser Met Gly Phe Thr Pro Leu Gly Gly Leu Ala Met Gly Thr Arg
225 230 235 240
Ser Gly Asp Met Asp Pro Ala Val Val Thr Phe Leu Met Asp Lys Leu
245 250 255
Asn Ile Asn Ala Ser Glu Val Asn Asn Leu Leu Asn Lys Lys Ser Gly
260 265 270
Ile Glu Gly Leu Ser Gly Ile Ser Ser Asp Met Arg Asp Ile Lys Lys
275 280 285
Gly Asn Tyr Val Asp Lys Asp Pro Lys Ala Met Leu Ala Tyr Ser Val
290 295 300
Phe Asn Tyr Lys Ile Lys Gln Phe Ile Gly Ser Tyr Thr Ala Val Met
305 310 315 320
Asn Gly Leu Asp Cys Leu Val Phe Thr Gly Gly Ile Gly Glu Asn Ser
325 330 335
Phe Glu Asn Arg Arg Glu Ile Cys Lys Asn Met Asp Tyr Leu Gly Ile
340 345 350
Lys Ile Asp Asp Lys Lys Asn Asp Glu Thr Met Gly Ile Pro Met Asp
355 360 365
Ile Ser Ala Glu Gly Ser Lys Val Arg Val Leu Val Ile Pro Thr Asn
370 375 380
Glu Glu Leu Met Ile Ala Arg Asp Thr Lys Asp Ile Val Gly Lys Leu
385 390 395 400
Lys
<210> 138
<211> 1206
<212> DNA
<213> Clostridium acetobutylicum
<400> 138
atgaaaaact tagttattaa ctgcggtagt tcatcaatca aataccagtt tatagatatg 60
aaggatgaaa ctgtactcgc taaaggatta gttgaaagaa ttggaataaa aggatctgta 120
ataacccata aagtaaatgg agaaaaatat gttacagaaa ctcctatgga agatcataaa 180
aaggctataa agcttgtatt agatgcttta ttaaatgatg aatatggtgt tataaaaaat 240
attgatgaga tatcagcagt aggacacaga atcgttcatg gtggagaaaa atatgcaaac 300
tcagttttaa tagatgaaga tgttatgaag tctatagaag attgtgtgag tcttgcaccg 360
cttcataatc caccacacat aataggaatt aatgcttgca aggaattaat gccaaacgtt 420
cctatggttg cagtatttga tacagcattc catcaaacta tacctgatta tgcatatatg 480
tatgctattc catatgaata ttatgataaa tacaaaataa gaaaatatgg ttttcatgga 540
acatcacaca aatatgtatc aagaacagct gcagaattta taggtaaaaa agtagaagat 600
ttaaaaatgg tagtatgcca tatgggaaat ggtgctagta ttacagctgt agaaaatggt 660
aaatcagtag atacaagtat gggatttact cctcttggcg gtcttgctat gggaactaga 720
agtggagata tggacccagc agtagtaact tttttaatgg ataaattaaa tataaatgct 780
tctgaagtaa ataatctatt aaataaaaag tcaggtattg aaggcttaag tggaataagc 840
agcgatatgc gtgatattaa aaaaggaaac tatgtagata aagaccctaa agctatgcta 900
gcttacagtg tatttaacta taaaataaag caatttatag gttcatatac tgcagttatg 960
aatggattag actgtttagt attcactggt ggaataggtg aaaattcatt tgaaaataga 1020
agagaaatat gcaaaaacat ggattatcta ggaataaaaa ttgacgataa gaaaaatgat 1080
gaaactatgg gaataccaat ggatataagt gcagaaggtt ctaaagttag ggtacttgta 1140
attccaacta atgaggagtt aatgattgca agggatacca aagatatagt aggcaagtta 1200
aaataa 1206
<210> 139
<211> 400
<212> PRT
<213> Thermoanaerobacterium saccharolyticum
<400> 139
Met Lys Ile Leu Val Ile Asn Cys Gly Ser Ser Ser Leu Lys Tyr Gln
1 5 10 15
Leu Ile Glu Ser Thr Asp Gly Asn Val Leu Ala Lys Gly Leu Ala Glu
20 25 30
Arg Ile Gly Ile Asn Asp Ser Met Leu Thr His Asn Ala Asn Gly Glu
35 40 45
Lys Ile Lys Ile Lys Lys Asp Met Lys Asp His Lys Asp Ala Ile Lys
50 55 60
Leu Val Leu Asp Ala Leu Val Asn Ser Asp Tyr Gly Val Ile Lys Asp
65 70 75 80
Met Ser Glu Ile Asp Ala Val Gly His Arg Val Val His Gly Gly Glu
85 90 95
Ser Phe Thr Ser Ser Val Leu Ile Asn Asp Glu Val Leu Lys Ala Ile
100 105 110
Thr Asp Cys Ile Glu Leu Ala Pro Leu His Asn Pro Ala Asn Ile Glu
115 120 125
Gly Ile Lys Ala Cys Gln Gln Ile Met Pro Asn Val Pro Met Val Ala
130 135 140
Val Phe Asp Thr Ala Phe His Gln Thr Met Pro Asp Tyr Ala Tyr Leu
145 150 155 160
Tyr Pro Ile Pro Tyr Glu Tyr Tyr Thr Lys Tyr Arg Ile Arg Arg Tyr
165 170 175
Gly Phe His Gly Thr Ser His Lys Tyr Val Ser Asn Arg Ala Ala Glu
180 185 190
Ile Leu Asn Lys Pro Ile Glu Asp Leu Lys Ile Ile Thr Cys His Leu
195 200 205
Gly Asn Gly Ser Ser Ile Ala Ala Val Lys Tyr Gly Lys Ser Ile Asp
210 215 220
Thr Ser Met Gly Phe Thr Pro Leu Glu Gly Leu Ala Met Gly Thr Arg
225 230 235 240
Ser Gly Ser Ile Asp Pro Ser Ile Ile Ser Tyr Leu Met Glu Lys Glu
245 250 255
Asn Ile Ser Ala Glu Glu Val Val Asn Ile Leu Asn Lys Lys Ser Gly
260 265 270
Val Tyr Gly Ile Ser Gly Ile Ser Ser Asp Phe Arg Asp Leu Glu Asp
275 280 285
Ala Ala Phe Lys Asn Gly Asp Glu Arg Ala Gln Leu Ala Leu Asn Val
290 295 300
Phe Ala Tyr Arg Val Lys Lys Thr Ile Gly Ala Tyr Ala Ala Ala Met
305 310 315 320
Gly Gly Val Asp Val Ile Val Phe Thr Ala Gly Val Gly Glu Asn Gly
325 330 335
Pro Glu Ile Arg Glu Phe Ile Leu Asp Gly Leu Glu Phe Leu Gly Phe
340 345 350
Ser Leu Asp Lys Glu Lys Asn Lys Val Arg Gly Lys Glu Thr Ile Ile
355 360 365
Ser Thr Pro Asn Ser Lys Val Ser Val Met Val Val Pro Thr Asn Glu
370 375 380
Glu Tyr Met Ile Ala Lys Asp Thr Glu Lys Ile Val Lys Ser Ile Lys
385 390 395 400
<210> 140
<211> 1203
<212> DNA
<213> Thermoanaerobacterium saccharolyticum
<400> 140
atgaaaatac tggttattaa ttgcggaagt tcttcgctaa aatatcaact gattgaatca 60
actgatggaa atgtgttggc aaaaggcctt gctgaaagaa tcggcataaa tgattccatg 120
ttgacacata atgctaacgg agaaaaaatc aagataaaaa aagacatgaa agatcacaaa 180
gacgcaataa aattggtttt agatgctttg gtaaacagtg actacggcgt tataaaagat 240
atgtctgaga tagatgctgt aggacataga gttgttcacg gaggagaatc ttttacatca 300
tcagttctca taaatgatga agtgttaaaa gcgataacag attgcataga attagctcca 360
ctgcacaatc ctgctaatat agaaggaatt aaagcttgcc agcaaatcat gccaaacgtt 420
ccaatggtgg cggtatttga tacagccttt catcagacaa tgcctgatta tgcatatctt 480
tatccaatac cttatgaata ctacacaaag tacaggatta gaagatatgg atttcatggc 540
acatcgcata aatatgtttc aaatagggct gcagagattt tgaataaacc tattgaagat 600
ttgaaaatca taacttgtca tcttggaaat ggctccagca ttgctgctgt caaatatggt 660
aaatcaattg acacaagcat gggatttaca ccattagaag gtttggctat gggtacacga 720
tctggaagca tagacccatc catcatttcg tatcttatgg aaaaagaaaa tataagcgct 780
gaagaagtag taaatatatt aaataaaaaa tctggtgttt acggtatttc aggaataagc 840
agcgatttta gagacttaga agatgccgcc tttaaaaatg gagatgaaag agctcagttg 900
gctttaaatg tgtttgcata tcgagtaaag aagacgattg gcgcttatgc agcagctatg 960
ggaggcgtcg atgtcattgt atttacagca ggtgttggtg aaaatggtcc tgagatacga 1020
gaatttatac ttgatggatt agagttttta gggttcagct tggataaaga aaaaaataaa 1080
gtcagaggaa aagaaactat tatatctacg ccgaattcaa aagttagcgt gatggttgtg 1140
cctactaatg aagaatacat gattgctaaa gatactgaaa agattgtaaa gagtataaaa 1200
tag 1203
<210> 141
<211> 2862
<212> DNA
<213> Yarrowia lipolytica
<400> 141
atgattgaag gaatctcctt tgcgtcgttt gtgacacacg aaaaacccaa gtttgtgcgc 60
gccctggact tctacaaggc cctgggcttc ctccccacca aggagtacaa gcacggaaca 120
gaccaccatg ccaccgacga agaaggcgcg ggctcgatcc aggaggtctg gctcacgtcg 180
tcgcgagccg gtgtgccgtc tgtgaccgtc aaattgcgac tctcccgcca cggaaacgaa 240
cacgtgtctc tccccaacct caaacacgac tggcggtcgc tggtgccctc gctggtgtac 300
tacgcccccg acctggacgc ggtgcgagcc gcaatcaccc cgttcttaca cgaggaccac 360
tccacgcttc tggaacgccc ttcccacaca aacttcatcg agctgtacgc cattgacccc 420
atgggaaacc tcgtgggctt ttcccgacga gaaaaccctt actcgtcagc gatgcagaaa 480
cccttctctg ctgacgacat tggcccgcag aacttctcaa agcccaatga aaccaaaatc 540
aagggcaaga agcgtatcgg tgtcatgacc tccggaggag acgcccccgg catgtgtgcg 600
gccgtgcgag ctgtggtccg agccggaatt gcccgcggct gcgaggtcta cgctgtccga 660
gagggctatg agggtcttgt caagggtggc gacctcattg agcccctgtc atgggaagac 720
gtacggggct ggctctccct gggaggaacc cttattggaa ctgctcgatg caaggagttc 780
cgagaacgag agggccgact ggcaggagcc ctcaacatgg tcaagaacgg cattgacgcg 840
ctcattgtca ttggcggaga cggctcgttg accggagccg atctgttccg agaagaatgg 900
ccgtctctaa tcgaggagct agtcaccaat ggctccatca ccgccgagca ggccgaacga 960
caccgacacc tcgacatttg cggtatggta ggctccatcg acaacgacat ggctaccacc 1020
gacgtcacca ttggtgctta ctcatcgctc gaccgaatct gcgagctggt agacttcatc 1080
gatgccaccg cccagtcgca ctcgcgagcc tttgtcgtcg aggttatggg tcggcactgt 1140
ggctggctgg ctctcatggc cggcactgct accggcgccg actacatttt tatccccgag 1200
gctgctcccg atgctactca gtgggccgag aagatgaccc gtgtcgtcaa gagacaccga 1260
agccagggca agcgaaagac cgtggtcatt gtcgccgagg gcgcaatcga ctcggacctc 1320
aaccccatca ctgccaagat ggtcaaggat gtgctagacg gcattggact cgatactcga 1380
atctccaccc tgggtcacgt acagcgagga ggtcccccag ttgccgctga tagagttctg 1440
gcttcactgc agggtgtgga ggctatcgac gccatcctgt ctctcacccc agagacgccc 1500
agtcccatga ttgctctcaa cgagaacaaa atcacccgca agccgctcgt ggagtctgta 1560
gctctcacca agaaggttgc cgatgccatt ggcaacaagg actttgccga ggccatgcgg 1620
cttcgaaacc ccgagtttgt ggagcaattg cagggtttcc tgctcacaaa ctctgctgac 1680
aaggaccggc cccaggagcc tgccaaggat cccctgcgag tcgccattgt gtgcactggc 1740
gctcctgctg gcggaatgaa cgctgccatc cgatctgctg ttctgtacgg tcttgctcga 1800
ggccaccaaa tgtttgccat ccacaatgga tggtccggcc tcgtcaagaa tggtgacgac 1860
gcggtgcggg agctgacttg gctcgaggtc gagcccctgt gtcagaaggg tggctgtgag 1920
attggtacta accgatctct gcccgaatgt gatcttggaa tgattgcata ccactttcag 1980
cgacaacggt ttgacggtct aatcgtcatt ggtggttttg aggctttccg agcgctgaat 2040
cagctcgacg atgcccgtca cgcctaccct gctcttcgaa tccccatggt tggtattcct 2100
gccaccattt cgaacaacgt gcctggaacg gactattctc ttggagccga cacttgtctc 2160
aactctctgg ttcagtactg cgacgtgctc aagacgtctg cttctgccac tcgtctgcgt 2220
ctgtttgtgg tcgaggtgca gggtggaaac tctggttaca tcgccaccgt ggctggtttg 2280
atcaccggcg cctatgtggt gtacacaccc gagagcggta tcaacctgcg acttcttcag 2340
cacgacattt cctaccttaa ggatactttt gctcatcagg cggacgtgaa ccgaaccgga 2400
aagctgcttc tgcgaaacga gcggtcatcc aacgtgttca ccactgatgt catcaccggc 2460
atcatcaacg aggaggccaa gggttcattt gacgcgcgaa ccgccatccc tggccatgtg 2520
cagcagggag gacacccctc tcctaccgat cgagtgcgtg ctcagcgatt tgccatcaag 2580
gccgtgcagt ttattgaaga gcaccacggc tccaaaaaca atgccgatca ctgtgtgatt 2640
ctcggtgtgc ggggctccaa gttcaagtac acctctgtgt cgcatctgta cgcccataag 2700
actgagcacg gggctcgacg gcccaagcat tcctactggc acgcgattgg cgacattgcc 2760
aacatgctgg tgggtcgaaa ggcgcctcct ctgcccgaga ctctcaacga cgagattgag 2820
aagaacattg cgaaggagca gggtattatt gatccttgtt ag 2862
<210> 142
<211> 3062
<212> DNA
<213> Yarrowia lipolytica
<400> 142
aaactctgat atatatatag agagacgtat cccaacagtt gatagtcgac aaacgcaaaa 60
cagacggaca ctgaaccccc cgcgcttcaa aacaccgaca atgattgaag gaatctcctt 120
tgcgtcgttt gtgacacacg aaaaacccaa gtttgtgcgc gccctggact tctacaaggc 180
cctgggcttc ctccccacca aggagtacaa gcacggaaca gaccaccatg ccaccgacga 240
agaaggcgcg ggctcgatcc aggaggtctg gctcacgtcg tcgcgagccg gtgtgccgtc 300
tgtgaccgtc aaattgcgac tctcccgcca cggaaacgaa cacgtgtctc tccccaacct 360
caaacacgac tggcggtcgc tggtgccctc gctggtgtac tacgcccccg acctggacgc 420
ggtgcgagcc gcaatcaccc cgttcttaca cgaggaccac tccacgcttc tggaacgccc 480
ttcccacaca aacttcatcg agctgtacgc cattgacccc atgggaaacc tcgtgggctt 540
ttcccgacga gaaaaccctt actcgtcagc gatgcagaaa cccttctctg ctgacgacat 600
tggcccgcag aacttctcaa agcccaatga aaccaaaatc aagggcaaga agcgtatcgg 660
tgtcatgacc tccggaggag acgcccccgg catgtgtgcg gccgtgcgag ctgtggtccg 720
agccggaatt gcccgcggct gcgaggtcta cgctgtccga gagggctatg agggtcttgt 780
caagggtggc gacctcattg agcccctgtc atgggaagac gtacggggct ggctctccct 840
gggaggaacc cttattggaa ctgctcgatg caaggagttc cgagaacgag agggccgact 900
ggcaggagcc ctcaacatgg tcaagaacgg cattgacgcg ctcattgtca ttggcggaga 960
cggctcgttg accggagccg atctgttccg agaagaatgg ccgtctctaa tcgaggagct 1020
agtcaccaat ggctccatca ccgccgagca ggccgaacga caccgacacc tcgacatttg 1080
cggtatggta ggctccatcg acaacgacat ggctaccacc gacgtcacca ttggtgctta 1140
ctcatcgctc gaccgaatct gcgagctggt agacttcatc gatgccaccg cccagtcgca 1200
ctcgcgagcc tttgtcgtcg aggttatggg tcggcactgt ggctggctgg ctctcatggc 1260
cggcactgct accggcgccg actacatttt tatccccgag gctgctcccg atgctactca 1320
gtgggccgag aagatgaccc gtgtcgtcaa gagacaccga agccagggca agcgaaagac 1380
cgtggtcatt gtcgccgagg gcgcaatcga ctcggacctc aaccccatca ctgccaagat 1440
ggtcaaggat gtgctagacg gcattggact cgatactcga atctccaccc tgggtcacgt 1500
acagcgagga ggtcccccag ttgccgctga tagagttctg gcttcactgc agggtgtgga 1560
ggctatcgac gccatcctgt ctctcacccc agagacgccc agtcccatga ttgctctcaa 1620
cgagaacaaa atcacccgca agccgctcgt ggagtctgta gctctcacca agaaggttgc 1680
cgatgccatt ggcaacaagg actttgccga ggccatgcgg cttcgaaacc ccgagtttgt 1740
ggagcaattg cagggtttcc tgctcacaaa ctctgctgac aaggaccggc cccaggagcc 1800
tgccaaggat cccctgcgag tcgccattgt gtgcactggc gctcctgctg gcggaatgaa 1860
cgctgccatc cgatctgctg ttctgtacgg tcttgctcga ggccaccaaa tgtttgccat 1920
ccacaatgga tggtccggcc tcgtcaagaa tggtgacgac gcggtgcggg agctgacttg 1980
gctcgaggtc gagcccctgt gtcagaaggg tggctgtgag attggtacta accgatctct 2040
gcccgaatgt gatcttggaa tgattgcata ccactttcag cgacaacggt ttgacggtct 2100
aatcgtcatt ggtggttttg aggctttccg agcgctgaat cagctcgacg atgcccgtca 2160
cgcctaccct gctcttcgaa tccccatggt tggtattcct gccaccattt cgaacaacgt 2220
gcctggaacg gactattctc ttggagccga cacttgtctc aactctctgg ttcagtactg 2280
cgacgtgctc aagacgtctg cttctgccac tcgtctgcgt ctgtttgtgg tcgaggtgca 2340
gggtggaaac tctggttaca tcgccaccgt ggctggtttg atcaccggcg cctatgtggt 2400
gtacacaccc gagagcggta tcaacctgcg acttcttcag cacgacattt cctaccttaa 2460
ggatactttt gctcatcagg cggacgtgaa ccgaaccgga aagctgcttc tgcgaaacga 2520
gcggtcatcc aacgtgttca ccactgatgt catcaccggc atcatcaacg aggaggccaa 2580
gggttcattt gacgcgcgaa ccgccatccc tggccatgtg cagcagggag gacacccctc 2640
tcctaccgat cgagtgcgtg ctcagcgatt tgccatcaag gccgtgcagt ttattgaaga 2700
gcaccacggc tccaaaaaca atgccgatca ctgtgtgatt ctcggtgtgc ggggctccaa 2760
gttcaagtac acctctgtgt cgcatctgta cgcccataag actgagcacg gggctcgacg 2820
gcccaagcat tcctactggc acgcgattgg cgacattgcc aacatgctgg tgggtcgaaa 2880
ggcgcctcct ctgcccgaga ctctcaacga cgagattgag aagaacattg cgaaggagca 2940
gggtattatt gatccttgtt agggggtttg gtgttggaaa ttaggatatc tatttgatta 3000
atgtagcttg gttttggaca agaatgctga ttgatacatc cggtatcact tgtatacaac 3060
gt 3062
<210> 143
<211> 583
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成的多核苷酸
<400> 143
cgcaaaacag acggacactg aaccccccgc gcttcaaaac accgacaatg accactctgg 60
atgacaccgc ttaccgatac cgaacttccg ttcctggcga tgccgaggct attgaggctc 120
tggatggatc tttcaccact gacaccgttt tccgagtgac cgctactggc gacggcttca 180
ccctgcgaga ggtgcctgtc gaccctcctc tcaccaaggt tttccctgac gatgagtcgg 240
acgatgagtc tgacgctgga gaggacggcg accctgactc tcgaactttc gtggcttacg 300
gcgacgatgg agacctggcc ggctttgtgg tcgtttctta ctccggatgg aaccgacgac 360
tgaccgtgga ggacatcgag gtcgctcctg agcaccgagg tcatggtgtc ggacgagctc 420
tgatgggtct cgctactgag ttcgctcgag agcgaggtgc tggccacctg tggctcgagg 480
tcaccaacgt taacgcccct gctattcatg cctaccgacg aatgggtttt accctgtgtg 540
gcctcgatac tgccctgtac gacggaaccg cttccgatgg aga 583
<210> 144
<211> 2396
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成的多核苷酸
<400> 144
aacttccgtt cctggcgatg ccgaggctat tgaggctctg gatggatctt tcaccactga 60
caccgttttc cgagtgaccg ctactggcga cggcttcacc ctgcgagagg tgcctgtcga 120
ccctcctctc accaaggttt tccctgacga tgagtcggac gatgagtctg acgctggaga 180
ggacggcgac cctgactctc gaactttcgt ggcttacggc gacgatggag acctggccgg 240
ctttgtggtc gtttcttact ccggatggaa ccgacgactg accgtggagg acatcgaggt 300
cgctcctgag caccgaggtc atggtgtcgg acgagctctg atgggtctcg ctactgagtt 360
cgctcgagag cgaggtgctg gccacctgtg gctcgaggtc accaacgtta acgcccctgc 420
tattcatgcc taccgacgaa tgggttttac cctgtgtggc ctcgatactg ccctgtacga 480
cggaaccgct tccgatggag agcaggccct ctacatgtcg atgccctgcc cttaaacagg 540
ccccttttcc tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc 600
tcccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat 660
ttattttttt taatagttat gttagtatta agaacgttat ttatatttca aatttttctt 720
ttttttctgt acaaacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag 780
gttttgggac gctcgaaggc tttaatttgc agagaccggg ttggcggcgc atttgtgtcc 840
caaaaaacag ccccaattgc cccaattgac cccaaattga cccagtagcg ggcccaaccc 900
cggcgagagc ccccttctcc ccacatatca aacctccccc ggttcccaca cttgccgtta 960
agggcgtagg gtactgcagt ctggaatcta cgcttgttca gactttgtac tagtttcttt 1020
gtctggccat ccgggtaacc catgccggac gcaaaataga ctactgaaaa tttttttgct 1080
ttgtggttgg gactttagcc aagggtataa aagaccaccg tccccgaatt acctttcctc 1140
ttcttttctc tctctccttg tcaactcaca cccgaaatcg ttaagcattt ccttctgagt 1200
ataagaatca ttcaaaatgg cttcttaccc ttgccaccag cacgcttccg cttttgacca 1260
ggccgcccga tcccgaggac actccaaccg acgaaccgct ctgcgacccc gacgacagca 1320
ggaggctacc gaggttcgac tggagcagaa gatgcctact ctgctccgag tgtacatcga 1380
cggaccccac ggtatgggca agaccactac cactcagctg ctcgtcgccc tgggttcgcg 1440
agatgacatt gtttacgtgc ctgagcccat gacctactgg caggttctcg gagcttctga 1500
gactatcgcc aacatctaca ccactcagca tcgactggac cagggagaga tctccgctgg 1560
agatgccgct gtggtcatga cctcggccca gattactatg ggcatgcctt acgctgtcac 1620
cgacgctgtt ctggctcctc acatcggtgg agaggctgga tcttcccatg ctcctcctcc 1680
tgctctgacc ctcatcttcg atcgacaccc tattgccgct ctgctctgtt accccgccgc 1740
tcgatacctg atgggctcta tgacccctca ggccgtgctg gcttttgtcg ccctcatccc 1800
tcccaccctg cctggtacta acattgtgct gggtgctctc cctgaggacc gacatatcga 1860
tcgactcgct aagcgacagc gacctggaga gcgactggac ctcgctatgc tggccgctat 1920
tcgacgagtg tacggcctgc tcgctaacac cgtccgatac ctccagggtg gtggatcgtg 1980
gcgagaggac tggggacagc tgtctggtac cgctgtgcct cctcagggtg ctgagcctca 2040
gtccaacgct ggacctcgac cccacatcgg tgacaccctg ttcactctct ttcgagctcc 2100
tgagctgctc gctcctaacg gcgacctgta caacgtcttc gcctgggctc tggatgttct 2160
cgccaagcga ctccgaccta tgcacgtctt tattctggac tacgatcagt cgcccgctgg 2220
atgtcgagat gccctgctcc agctcacctc tggcatggtt cagactcatg tgaccactcc 2280
tggatccatc cccaccattt gcgatctggc tcgaactttc gcccgagaga tgggagaggc 2340
caactaaggg gtttggtgtt ggaaattagg atatctattt gattaatgta gcttgg 2396
<210> 145
<211> 189
<212> PRT
<213> Streptomyces noursei
<400> 145
Met Thr Thr Leu Asp Asp Thr Ala Tyr Arg Tyr Arg Thr Ser Val Pro
1 5 10 15
Gly Asp Ala Glu Ala Ile Glu Ala Leu Asp Gly Ser Phe Thr Thr Asp
20 25 30
Thr Val Phe Arg Val Thr Ala Thr Gly Asp Gly Phe Thr Leu Arg Glu
35 40 45
Val Pro Val Asp Pro Pro Leu Thr Lys Val Phe Pro Asp Asp Glu Ser
50 55 60
Asp Asp Glu Ser Asp Ala Gly Glu Asp Gly Asp Pro Asp Ser Arg Thr
65 70 75 80
Phe Val Ala Tyr Gly Asp Asp Gly Asp Leu Ala Gly Phe Val Val Val
85 90 95
Ser Tyr Ser Gly Trp Asn Arg Arg Leu Thr Val Glu Asp Ile Glu Val
100 105 110
Ala Pro Glu His Arg Gly His Gly Val Gly Arg Ala Leu Met Gly Leu
115 120 125
Ala Thr Glu Phe Ala Arg Glu Arg Gly Ala Gly His Leu Trp Leu Glu
130 135 140
Val Thr Asn Val Asn Ala Pro Ala Ile His Ala Tyr Arg Arg Met Gly
145 150 155 160
Phe Thr Leu Cys Gly Leu Asp Thr Ala Leu Tyr Asp Gly Thr Ala Ser
165 170 175
Asp Gly Glu Gln Ala Leu Tyr Met Ser Met Pro Cys Pro
180 185
<210> 146
<211> 570
<212> DNA
<213> Streptomyces noursei
<400> 146
atgaccactc tggatgacac cgcttaccga taccgaactt ccgttcctgg cgatgccgag 60
gctattgagg ctctggatgg atctttcacc actgacaccg ttttccgagt gaccgctact 120
ggcgacggct tcaccctgcg agaggtgcct gtcgaccctc ctctcaccaa ggttttccct 180
gacgatgagt cggacgatga gtctgacgct ggagaggacg gcgaccctga ctctcgaact 240
ttcgtggctt acggcgacga tggagacctg gccggctttg tggtcgtttc ttactccgga 300
tggaaccgac gactgaccgt ggaggacatc gaggtcgctc ctgagcaccg aggtcatggt 360
gtcggacgag ctctgatggg tctcgctact gagttcgctc gagagcgagg tgctggccac 420
ctgtggctcg aggtcaccaa cgttaacgcc cctgctattc atgcctaccg acgaatgggt 480
tttaccctgt gtggcctcga tactgccctg tacgacggaa ccgcttccga tggagagcag 540
gccctctaca tgtcgatgcc ctgcccttaa 570
<210> 147
<211> 189
<212> PRT
<213> Herpes simplex virus
<400> 147
Met Thr Thr Leu Asp Asp Thr Ala Tyr Arg Tyr Arg Thr Ser Val Pro
1 5 10 15
Gly Asp Ala Glu Ala Ile Glu Ala Leu Asp Gly Ser Phe Thr Thr Asp
20 25 30
Thr Val Phe Arg Val Thr Ala Thr Gly Asp Gly Phe Thr Leu Arg Glu
35 40 45
Val Pro Val Asp Pro Pro Leu Thr Lys Val Phe Pro Asp Asp Glu Ser
50 55 60
Asp Asp Glu Ser Asp Ala Gly Glu Asp Gly Asp Pro Asp Ser Arg Thr
65 70 75 80
Phe Val Ala Tyr Gly Asp Asp Gly Asp Leu Ala Gly Phe Val Val Val
85 90 95
Ser Tyr Ser Gly Trp Asn Arg Arg Leu Thr Val Glu Asp Ile Glu Val
100 105 110
Ala Pro Glu His Arg Gly His Gly Val Gly Arg Ala Leu Met Gly Leu
115 120 125
Ala Thr Glu Phe Ala Arg Glu Arg Gly Ala Gly His Leu Trp Leu Glu
130 135 140
Val Thr Asn Val Asn Ala Pro Ala Ile His Ala Tyr Arg Arg Met Gly
145 150 155 160
Phe Thr Leu Cys Gly Leu Asp Thr Ala Leu Tyr Asp Gly Thr Ala Ser
165 170 175
Asp Gly Glu Gln Ala Leu Tyr Met Ser Met Pro Cys Pro
180 185
<210> 148
<211> 1131
<212> DNA
<213> Herpes simplex virus
<400> 148
atggcttctt acccttgcca ccagcacgct tccgcttttg accaggccgc ccgatcccga 60
ggacactcca accgacgaac cgctctgcga ccccgacgac agcaggaggc taccgaggtt 120
cgactggagc agaagatgcc tactctgctc cgagtgtaca tcgacggacc ccacggtatg 180
ggcaagacca ctaccactca gctgctcgtc gccctgggtt cgcgagatga cattgtttac 240
gtgcctgagc ccatgaccta ctggcaggtt ctcggagctt ctgagactat cgccaacatc 300
tacaccactc agcatcgact ggaccaggga gagatctccg ctggagatgc cgctgtggtc 360
atgacctcgg cccagattac tatgggcatg ccttacgctg tcaccgacgc tgttctggct 420
cctcacatcg gtggagaggc tggatcttcc catgctcctc ctcctgctct gaccctcatc 480
ttcgatcgac accctattgc cgctctgctc tgttaccccg ccgctcgata cctgatgggc 540
tctatgaccc ctcaggccgt gctggctttt gtcgccctca tccctcccac cctgcctggt 600
actaacattg tgctgggtgc tctccctgag gaccgacata tcgatcgact cgctaagcga 660
cagcgacctg gagagcgact ggacctcgct atgctggccg ctattcgacg agtgtacggc 720
ctgctcgcta acaccgtccg atacctccag ggtggtggat cgtggcgaga ggactgggga 780
cagctgtctg gtaccgctgt gcctcctcag ggtgctgagc ctcagtccaa cgctggacct 840
cgaccccaca tcggtgacac cctgttcact ctctttcgag ctcctgagct gctcgctcct 900
aacggcgacc tgtacaacgt cttcgcctgg gctctggatg ttctcgccaa gcgactccga 960
cctatgcacg tctttattct ggactacgat cagtcgcccg ctggatgtcg agatgccctg 1020
ctccagctca cctctggcat ggttcagact catgtgacca ctcctggatc catccccacc 1080
atttgcgatc tggctcgaac tttcgcccga gagatgggag aggccaacta a 1131
<210> 149
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成的多核苷酸引物
<400> 149
cgcaaaacag acggacactg aaccccccgc gcttcaaaac accgacaatg accactctgg 60
atgacacc 68
<210> 150
<211> 72
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成的多核苷酸引物
<400> 150
ccaagctaca ttaatcaaat agatatccta atttccaaca ccaaacccct tagttggcct 60
ctcccatctc tc 72
<210> 151
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成的多核苷酸引物
<400> 151
aacttccgtt cctggcgatg ccg 23
<210> 152
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成的多核苷酸引物
<400> 152
tctccatcgg aagcggttcc gtc 23
<210> 153
<211> 974
<212> PRT
<213> Arxula adeninivorans
<400> 153
Met Gly Lys Ala Lys Leu Val Cys Gly Val Thr Phe Ile Ser Phe Pro
1 5 10 15
Thr Ser Asp Gln Asp Leu Tyr Gln Lys Ala Val Asp Phe Tyr Gln Arg
20 25 30
Arg Leu Gly Phe Ser Val Ile Gln Glu Tyr Asp His Thr Ser Met Ser
35 40 45
Val Gly Val Lys Ser Val Ala Ser His Cys Ala Ser Ser Val Arg Glu
50 55 60
Thr Trp Leu Asn Val Leu Pro Asp Asp Ala Gln Gln Ala Leu Glu Thr
65 70 75 80
Ala Ala Pro Gly Thr Thr Ile Lys Ile Arg Leu Thr Pro Asp Gly Pro
85 90 95
Gly Ala Ala Gln Val Glu Ala Lys Ile Asp Ala Val Ile Asp Lys Tyr
100 105 110
Lys Asp Lys Asp Trp Arg Gly Ala Ser Ala Ala Met Val Phe His Thr
115 120 125
Glu Asp Leu Ala Asp Val Lys Ser Asp Leu Gly Asp Tyr Pro Ala Glu
130 135 140
Tyr Leu Ser Gly Val Pro Ser Gly Gln Gln Ala Ala Glu Ala Tyr Val
145 150 155 160
Leu Asp Pro Leu Gly Asn Leu Ile Gly Phe Thr Thr Arg His Asn Pro
165 170 175
Phe Ser Ser His Asp Arg Pro Ala Thr Leu Ser Gly Asp Gly Gln Ser
180 185 190
Thr Ser Gly Gly Ile Gln Gln Asn Ser Ser Ser Ser Val Gly Thr Thr
195 200 205
Gly Val Ala Thr Pro Ala Leu Thr Ser Ser Val Gly Gly Gly Ser Lys
210 215 220
Lys Lys Lys Ile Ala Val Met Thr Ser Gly Gly Asp Ala Pro Gly Met
225 230 235 240
Asn Ala Val Val Arg Ala Val Val Arg Thr Ala Ile Ala Arg Gly Cys
245 250 255
Asp Ala Tyr Ala Val Tyr Glu Gly Tyr Gln Gly Leu Val Thr Gly Gly
260 265 270
Asp Tyr Ile Lys Lys Thr Ser Trp Asp Glu Val Arg Gly Tyr Leu Ala
275 280 285
Leu Gly Gly Thr Lys Ile Gly Thr Ala Arg Cys Lys Glu Phe Arg Glu
290 295 300
Arg Asp Gly Arg Leu Gln Ala Cys Val Asn Met Ile Lys Ala Gly Ile
305 310 315 320
Asp Gly Leu Ile Val Cys Gly Gly Asp Gly Ser Leu Thr Gly Ala Asp
325 330 335
Arg Phe Arg Glu Glu Trp Pro Ser Leu Val Gln Glu Ala Lys Glu Lys
340 345 350
Asn Leu Val Thr Pro Gln Glu Leu Ala Gly His Glu His Leu Tyr Ile
355 360 365
Cys Gly Leu Val Gly Ser Ile Asp Asn Asp Met Ser Asn Thr Asp Ala
370 375 380
Thr Ile Gly Ala Tyr Ser Ser Leu Asp Arg Ile Cys Gln Ala Val Asp
385 390 395 400
Phe Ile Asp Ala Thr Ala Glu Ser His Ser Arg Ala Phe Val Ile Glu
405 410 415
Val Met Gly Arg His Cys Gly Trp Leu Gly Leu Met Ala Gly Leu Ala
420 425 430
Thr Gly Ala Asp Tyr Ile Phe Ile Pro Glu Arg Pro Pro Ser Lys Asp
435 440 445
Glu Trp Arg Arg Lys Met Ser Asp Val Val Val Arg His Arg Lys Asn
450 455 460
Gly Met Arg Arg Thr Ile Val Ile Val Ala Glu Gly Ala Ile Asp Thr
465 470 475 480
Glu Leu Asn Ala Ile Thr Pro Val Met Val Lys Asp Gln Leu Val Asp
485 490 495
Leu Gly Leu Asp Thr Arg Ile Thr Thr Leu Gly His Val Gln Arg Gly
500 505 510
Gly Thr Ala Val Ala Phe Asp Arg Met Leu Ala Thr Leu Gln Gly Val
515 520 525
Glu Ala Val Asp Ala Ile Leu Thr Ser Thr Pro Glu Thr Pro Ser Pro
530 535 540
Met Ile Gly Leu Thr Glu Asn Lys Val Val Arg Arg Asp Leu Ile Glu
545 550 555 560
Ser Val Lys Leu Thr Gly Ser Val Ala Glu Ala Ile Asn Arg Lys Asp
565 570 575
Phe Asp Gly Ala Met Gly Leu Arg Asp Ser Glu Phe Cys Glu His Leu
580 585 590
Asp Asn Phe Tyr Met Ile Asn Ser Ala Asp Lys Asp Lys Pro Leu Ser
595 600 605
Pro Ala Glu Lys Arg Leu Lys Val Ala Ile Val Cys Ile Gly Ala Pro
610 615 620
Ala Gly Gly Met Asn Ala Ala Ile Arg Ala Ala Ala Cys Tyr Cys Phe
625 630 635 640
Ala Arg Gly His Thr Pro Tyr Ala Ile His Asn Gly Phe Thr Gly Leu
645 650 655
Ser Arg His Glu Ser Val Lys Glu Leu Asn Trp Leu Glu Val Glu Gln
660 665 670
Tyr Gly Asn Gln Gly Gly Cys Glu Ile Gly Thr Asn Arg His Thr Pro
675 680 685
Asp Val Asp Leu Gly Met Val Ala Tyr Tyr Phe Gln Lys Tyr Glu Phe
690 695 700
Asp Gly Leu Val Ile Ile Gly Gly Phe Glu Ala Phe Asn Ser Leu His
705 710 715 720
Ile Leu Ser Glu Ala Arg Lys Ser Tyr Pro Ala Phe Arg Met Pro Met
725 730 735
Val Cys Leu Pro Ala Thr Ile Ser Asn Asn Val Pro Gly Thr Glu Tyr
740 745 750
Ser Leu Gly Thr Asp Thr Cys Leu Asn Ala Leu Val Gln Tyr Cys Asp
755 760 765
Val Ile Lys Gln Ser Ala Ser Ser Thr Arg Arg Arg Ala Phe Val Val
770 775 780
Glu Val Gln Gly Gly Asn Ser Gly Tyr Val Ala Ser Tyr Ala Gly Leu
785 790 795 800
Val Thr Gly Ala His Val Val Tyr Thr Pro Glu Glu Gly Ile Ser Leu
805 810 815
Lys Gln Leu Ser Ala Asp Ile Glu Phe Leu Lys His Gln Phe Ala His
820 825 830
Asp Ser Gly Arg Asn Arg Ala Gly Arg Leu Ile Leu Arg Asn Glu Lys
835 840 845
Ala Ser Lys Thr Phe Asn Thr Asp Val Leu Val Ser Ile Leu Gln Asn
850 855 860
Glu Gly Gly Ser Gln Phe Glu Ala Arg Glu Ala Ile Pro Gly His Val
865 870 875 880
Gln Gln Gly Gly Thr Pro Ser Pro Met Asp Arg Ala Arg Ala Ala Arg
885 890 895
Phe Ala Val Arg Cys Ile Gln Phe Ile Glu Asp Gln Gln Glu Ile Arg
900 905 910
Ser Lys Pro Thr Pro Asp Gln Asp Ser Met Tyr Ser Val Val Gly Val
915 920 925
Arg Lys Ala Lys Leu Val Tyr Thr Pro Val Arg Glu Leu Trp Asp Phe
930 935 940
Glu Thr Glu Val Thr Ser Arg Arg Pro Asn Lys Ile His Trp Asn Arg
945 950 955 960
Met Ile Thr Ile Ala Asp Met Leu Val Gly Arg Thr Lys Val
965 970
<210> 154
<211> 2925
<212> DNA
<213> Arxula adeninivorans
<400> 154
atgggtaaag caaagctggt gtgtggagtg acgtttattt cgtttcccac gtcggaccag 60
gacctgtacc aaaaggcagt ggacttttac cagcgacggt taggattctc ggtgatccag 120
gagtatgacc atacgtccat gtctgtggga gtcaagtcgg tggcttcgca ttgtgcgtcg 180
tcagttcgag aaacttggct caatgtgctt cccgacgacg ctcagcaggc gttggaaacg 240
gccgctccgg gaactacaat taagatccgc ctgactcctg acggtccagg tgctgcgcag 300
gtagaggcca agattgatgc cgttattgac aagtacaagg acaaggactg gcgaggagct 360
tcggcagcaa tggtattcca taccgaagat ttggctgacg tcaagtctga tttgggcgac 420
tatcctgccg aatacctgtc gggagtgccc agcggacagc aggctgcgga ggcctatgtt 480
ttagaccctc tgggaaacct cattggtttc accactagac acaatccctt ttctagccac 540
gaccgccctg ctactttgtc tggggacggc cagtctactt ctggtggtat tcagcagaat 600
tcttcgtcgt cggttggtac tactggagta gctacccctg ccctcacttc gtccgtcgga 660
ggaggcagca agaagaagaa gattgccgtc atgacctccg gaggtgatgc ccccggaatg 720
aacgctgtag tgcgagcggt agttagaacc gcaattgctc gcggatgcga cgcttacgct 780
gtatacgaag gataccaggg tctagtgacc ggcggcgact atattaaaaa gacttcctgg 840
gacgaagtcc gtggatacct ggctctggga ggtaccaaga ttggaactgc gcgatgcaag 900
gagttccggg aacgcgacgg tcgtctgcag gcgtgcgtca acatgatcaa ggctggaatt 960
gacggtctca ttgtgtgcgg aggagatggt tcgctcactg gtgccgaccg attccgagag 1020
gaatggccta gtttggtcca ggaggccaag gaaaagaacc tggtcacccc ccaggagctc 1080
gcgggccacg aacacttgta catttgcggt ctggtgggtt ccattgacaa cgacatgagt 1140
aacaccgatg ctacaattgg agcgtactct tcgctggacc gaatctgcca ggcagtagac 1200
tttatcgatg ccactgccga gtcccattcg cgagcgtttg tcattgaggt catgggtcga 1260
cactgcggtt ggctgggtct gatggccggt ctggccactg gcgctgacta cattttcatt 1320
cctgagcgtc ctccttccaa ggacgaatgg cgacgcaaga tgagcgatgt agtggtacgt 1380
caccgtaaga acggtatgcg tcgaaccatt gtcattgtag ctgagggtgc tattgacacc 1440
gagcttaacg ccattactcc tgtcatggtc aaagaccagc tggtggacct cggcctggac 1500
actcgtatca ctaccctggg tcacgtccag cgaggaggaa ccgcagttgc ctttgaccgt 1560
atgctggcta ctctgcaggg tgtggaagct gtggacgcta ttctcacttc tacccccgag 1620
actccctctc ctatgatcgg actgaccgag aacaaggttg tgcgccgaga cctgattgaa 1680
tcggtcaagc tcaccgggtc ggtggcagag gcaattaacc gtaaagactt tgatggcgcc 1740
atgggcctgc gagactctga gttctgcgaa cacctggaca atttctacat gatcaactcg 1800
gccgacaagg acaagcctct aagccctgcc gaaaagagac tcaaggtggc cattgtgtgc 1860
attggagctc ccgcgggagg aatgaacgct gccattcgag ctgctgcatg ctactgtttt 1920
gctcgtggcc acactccata cgccatccac aacggtttca ccggtctgtc tcgccacgag 1980
tcggtcaagg agctcaactg gctcgaggtg gagcagtacg gtaaccaggg aggttgcgag 2040
attggtacca accgtcacac ccccgacgtg gacctgggaa tggtggcata ctacttccag 2100
aagtatgagt ttgacggtct ggtcatcatt ggtggttttg aggcattcaa ctcgctgcat 2160
attctgtccg aggcgcgaaa gtcgtaccct gctttccgca tgccaatggt gtgtctgcct 2220
gccacaattt ctaacaatgt tcccggaaca gagtactctc tgggaaccga cacctgtctc 2280
aacgcgctgg tgcagtactg tgacgtgatc aagcagtctg cttcttctac tcgacgacga 2340
gcgtttgtgg tcgaggtcca gggaggaaac tctggttacg tagcttctta cgctggattg 2400
gttactggag ctcacgttgt gtacactcct gaggagggca tctccctcaa gcagctgtcg 2460
gctgacattg agttcctaaa gcatcagttt gctcacgaca gtggccgcaa ccgagccgga 2520
cgactgattc tgcgcaacga aaaggcttca aagacgttta acactgacgt tttggtgtcc 2580
attctgcaga atgaaggagg cagccagttt gaagctcgcg aggccattcc cggtcacgtc 2640
cagcagggag gtactccttc tcctatggac cgtgctcgtg ctgctcgatt tgctgtgcga 2700
tgtatccaat tcattgagga ccagcaggag attcgatcaa agcctactcc tgaccaggat 2760
tcaatgtact ctgtagtggg agtgcgaaag gcaaagcttg tctacacccc tgtccgggaa 2820
ctgtgggact ttgagaccga ggtcacctct cgccgaccaa acaagattca ctggaaccgc 2880
atgattacca ttgctgacat gcttgttggc cgaaccaagg tataa 2925
<210> 155
<211> 754
<212> PRT
<213> Arxula adeninivorans
<400> 155
Met Glu His Lys Thr Thr Arg Ala Leu Ser Glu Ala Leu Lys Ser Thr
1 5 10 15
Leu Ala Pro Pro Pro Ser Gln Ser Gly Asp Gly Glu Val Ser Pro His
20 25 30
Thr Val Pro Ala Ala Gly Tyr Gly Ser Ser Ser Ser Ser Gly Ala Gln
35 40 45
Ser Ser Glu Asn Glu Leu Gln Pro Glu Gly Ala Pro Gly Thr Ser Ser
50 55 60
Ser Phe Asn Arg Arg Pro Pro Ser Asp Thr Pro Leu Phe Ser Pro Arg
65 70 75 80
Ser Ala Ser Asp Leu Ser Arg Ile Pro Ile Thr Gln Pro Ser Ser Ser
85 90 95
Gly Ser Ser Arg Asn Asn Ser His Ser Asn Ser Pro Arg Ser Thr Ser
100 105 110
Ser Ile Pro Pro Leu His Leu Ser Ser Gln Arg Arg Ala Ala Thr Thr
115 120 125
Leu Asp Val Pro Gly Leu Thr Arg Ser Lys Ile Ser Pro Asp Gly Met
130 135 140
Ile Ala Ala Arg Asp Leu Glu Ser Lys Leu Val Ile Val Met Val Gly
145 150 155 160
Leu Pro Ala Arg Gly Lys Ser Tyr Val Thr Lys Lys Leu Cys Arg Tyr
165 170 175
Leu Asn Trp Gln Gln His Gly Ala Arg Ile Phe Asn Val Gly Asn Thr
180 185 190
Arg Arg Asn Ala Asp Arg Thr Val Gly Pro Ala Ser Arg Pro Leu Pro
195 200 205
Asp Glu Gly Val Ala Leu Ala Ser Pro Glu Gln Glu Ala Gln Ala His
210 215 220
Ala Glu His Glu Ala Lys Ala Leu Glu Ala Glu Met Arg Gly Asp Pro
225 230 235 240
Pro Asp His Thr Asp Ser Ala Asp Phe Phe Ser Pro Asp Asn Ala Gln
245 250 255
Thr Ser Gln Leu Arg Glu Lys Trp Ala Met Asp Thr Leu Asp Glu Leu
260 265 270
Leu Asp Tyr Ile Leu Asn Glu Asn Gly Ser Val Gly Val Leu Asp Ala
275 280 285
Thr Asn Thr Thr Arg Ala Arg Arg Lys Lys Val Leu Asp Arg Ile Arg
290 295 300
Glu Arg Thr Gly Gly Arg Leu Lys Val Leu Phe Leu Glu Ser Ile Cys
305 310 315 320
Thr Arg Ser Asp Ile Ile Asp Ala Asn Ile Arg Leu Lys Leu Ser Gly
325 330 335
Pro Asp Tyr Lys Asp Met Asp Gln Glu Lys Ala Leu Lys Asp Phe Val
340 345 350
Ala Arg Leu His Asn Tyr Glu Lys Val Tyr Glu Thr Ile Ser Asp Glu
355 360 365
Glu Glu Glu Asp Asp Asn Phe Gln Tyr Ile Lys Met Ile Asp Val Gly
370 375 380
Arg Lys Val Val Cys Tyr Asn Ile Gln Gly Phe Leu Ala Gly Gln Ala
385 390 395 400
Val Phe Phe Leu Leu Asn Phe Asn Leu Ala Glu Arg Gln Ile Trp Ile
405 410 415
Thr Arg His Gly Glu Ser Glu Asp Asn Ala Ala Gly Arg Ile Gly Gly
420 425 430
Asp Ala Pro Leu Thr Ala Arg Gly Glu Lys Phe Ala Lys Ala Leu Ala
435 440 445
Arg Phe Met Asp Phe Gln Lys Ser Glu Phe Arg Arg Lys Gln Leu Gln
450 455 460
Lys Phe Thr Asp Arg Val Arg Ile Leu Lys Lys Glu Gly Ser Ala Pro
465 470 475 480
Ser Thr Pro Leu Asn Glu Pro Glu Glu Pro Asn Phe Cys Val Trp Thr
485 490 495
Ser Met Met Lys Arg Ser Val Gln Thr Ala Gln Tyr Met Asp Glu Asp
500 505 510
Met Phe Ala Ile Lys Glu Met Arg Met Leu Asn Glu Leu Gly Ala Gly
515 520 525
Val Cys Glu Gly Met Thr Tyr Glu Glu Ile Ser Gln Thr Tyr Pro Glu
530 535 540
Glu Tyr Ala Ala Arg Ile Ala Asp Lys Ile Gln Tyr Arg Tyr Pro Gly
545 550 555 560
Ile Gly Gly Glu Ser Tyr Leu Asp Val Ile Asn Arg Leu Arg Pro Val
565 570 575
Ile Val Glu Met Glu Arg Met Glu Asp Asn Ala Leu Ile Ile Ala His
580 585 590
Arg Val Val Ala Arg Val Leu Leu Ala Tyr Phe Met Asn Leu Gly Arg
595 600 605
Asp Ala Ile Gly Asp Leu Asp Val Pro Leu His Thr Leu Tyr Met Leu
610 615 620
Glu Pro Lys Pro Tyr Gly Val Asp Trp Ala Val Tyr Glu Tyr Ser Glu
625 630 635 640
Glu Thr Asp Trp Phe Tyr Arg Val Pro Lys Glu Gln Ile Ser Ala Gln
645 650 655
Ile Gln Arg Leu Gln Arg Glu Ser Ser Gln Lys Arg Ser Asn Arg Gly
660 665 670
Ala Pro Ala Val Ile Gly Thr Gly Thr Ala Leu Gly Ser Thr Lys Glu
675 680 685
Arg Asn Phe Ser Val Val Pro Thr Gln Glu Asp Ala Ala Ala Ala Ser
690 695 700
Ala Ala Asn Asn Ile Ser Gln Leu Ser Leu Gly Ala Ser Ser Ala Pro
705 710 715 720
Ser Ser Arg Val Pro Leu Ala Asn Arg Arg Arg Arg Pro Ser Ala Val
725 730 735
Asp Asp Ile Gln Val Lys Gln Ala Asp Tyr His Asp Trp Glu Thr Ala
740 745 750
Ala His
<210> 156
<211> 2265
<212> DNA
<213> Arxula adeninivorans
<400> 156
atggagcaca agactactag agcgctatca gaagcattga aatcgacgct ggctcctccg 60
ccttctcaaa gtggcgatgg cgaagtgagc ccacacacgg tgccggcggc tgggtatggc 120
agctccagtt ctagtggagc gcagtcttcc gaaaatgaat tacagccaga aggagctcct 180
ggtaccagtt catcgtttaa ccgacggccg ccttccgaca ccccgctgtt ttcgcctcga 240
agcgcgtctg acttgtcccg aatccccatc acacagccat ctagttcggg gtcctctaga 300
aacaattccc attcaaactc ccccagaagt acttccagca ttcctccttt gcatctttca 360
tcccagcgta gggccgccac cactctagat gtgcctgggc tgacccgatc caagatctcc 420
cccgatggta tgatcgcggc aagagacttg gagtccaagc tagtaattgt aatggtaggc 480
cttccggcac gaggaaagtc gtatgtgaca aagaaactgt gccgatacct caactggcag 540
cagcatggcg cacgcatctt caatgttggc aacacccgtc gtaacgcaga ccgtacagta 600
ggtccagcat cccgccccct tcctgatgag ggcgtagcat tagcatcgcc agagcaagaa 660
gcgcaggcgc atgccgagca cgaggcaaag gctttggagg ctgaaatgag aggcgatcct 720
cctgatcata ctgactcggc cgactttttc agccctgaca acgctcagac ttcgcaattg 780
cgggagaagt gggcaatgga tactctagac gaattgcttg actatattct aaatgagaat 840
ggctcggttg gagtgctgga cgctaccaac accacccgtg ctcgacgaaa gaaggtgctg 900
gaccgaattc gtgaacgcac cggaggccga ctcaaggttc tgtttctgga gtccatttgc 960
actcgctcgg atataatcga tgcaaacatt cgtctcaagc tgtcgggtcc cgattacaaa 1020
gatatggacc aggagaaggc cctcaaggac ttcgtggctc gtctccacaa ctacgaaaag 1080
gtctacgaga ctattagcga cgaagaggaa gaggacgaca actttcaata tattaagatg 1140
attgacgtag gtcgcaaggt ggtgtgttac aacatccagg gattccttgc tggccaggca 1200
gtctttttcc tgctcaactt taacctggcc gagcgacaga tctggattac ccgtcatgga 1260
gagagtgaag ataatgccgc cggtcgaatt ggcggtgacg cacctctcac tgctcgagga 1320
gaaaagtttg caaaggccct ggctcgattc atggactttc agaagagcga attccgaaga 1380
aagcagctgc aaaagttcac cgaccgggtc cgaatactca agaaggaagg gtcggcacca 1440
tctactcctc ttaacgagcc tgaagagcca aacttctgcg tgtggacttc aatgatgaag 1500
agaagtgtgc agaccgctca atatatggac gaggacatgt ttgccatcaa ggagatgcgt 1560
atgctcaatg agcttggtgc gggagtgtgc gaaggaatga cttacgaaga gatttcccag 1620
acctaccctg aagagtacgc ggcaagaatc gctgacaaga ttcagtacag gtatccaggc 1680
attggcggag agtcctatct cgacgttatc aaccgactgc gtccagtgat tgtggagatg 1740
gagcgaatgg aggataatgc tctgatcatt gcccatagag tggtggccag ggttctgtta 1800
gcatacttta tgaacctggg ccgcgatgcc attggcgacc tagatgtgcc tcttcacacc 1860
ctgtacatgc tagaacccaa gccatatgga gtggattggg ccgtttatga atacagcgaa 1920
gaaaccgact ggttctacag agtgccaaag gagcaaatct ctgcccagat ccaacgtctc 1980
cagcgcgaga gttctcaaaa gagatccaat agaggcgccc ctgccgtcat tggcactggt 2040
actgctttag gatcaacaaa ggagagaaac ttcagtgttg ttcctaccca ggaggatgct 2100
gctgctgcat ctgccgccaa caacatatcg cagctctctc tcggtgcttc ctctgcacct 2160
tcatcaagag taccgctggc taaccgacgc aggcggccaa gcgctgtcga cgacattcaa 2220
gtcaaacaag ccgattatca tgactgggag acggccgccc actaa 2265
<210> 157
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成的多核苷酸引物
<400> 157
tcctaacagc tcgcaccgac 20
<210> 158
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成的多核苷酸引物
<400> 158
gggtcaatgg cgtacagctc 20
<210> 159
<211> 822
<212> DNA
<213> Saccharomyces cerevisiae
<400> 159
gatccaactg gcaccgctgg cttgaacaac aataccagcc ttccaacttc tgtaaataac 60
ggcggtacgc cagtgccacc agtaccgtta cctttcggta tacctccttt ccccatgttt 120
ccaatgccct tcatgcctcc aacggctact atcacaaatc ctcatcaagc tgacgcaagc 180
cctaagaaat gaataacaat actgacagta ctaaataatt gcctacttgg cttcacatac 240
gttgcatacg tcgatataga taataatgat aatgacagca ggattatcgt aatacgtaat 300
agttgaaaat ctcaaaaatg tgtgggtcat tacgtaaata atgataggaa tgggattctt 360
ctatttttcc tttttccatt ctagcagccg tcgggaaaac gtggcatcct ctctttcggg 420
ctcaattgga gtcacgctgc cgtgagcatc ctctctttcc atatctaaca actgagcacg 480
taaccaatgg aaaagcatga gcttagcgtt gctccaaaaa agtattggat ggttaatacc 540
atttgtctgt tctcttctga ctttgactcc tcaaaaaaaa aaaatctaca atcaacagat 600
cgcttcaatt acgccctcac aaaaactttt ttccttcttc ttcgcccacg ttaaatttta 660
tccctcatgt tgtctaacgg atttctgcac ttgatttatt ataaaaagac aaagacataa 720
tacttctcta tcaatttcag ttattgttct tccttgcgtt attcttctgt tcttcttttt 780
cttttgtcat atataaccat aaccaagtaa tacatattca aa 822
<210> 160
<211> 999
<212> DNA
<213> Yarrowia lipolytica
<400> 160
gagtttggcg cccgtttttt cgagccccac acgtttcggt gagtatgagc ggcggcagat 60
tcgagcgttt ccggtttccg cggctggacg agagcccatg atgggggctc ccaccaccag 120
caatcagggc cctgattaca cacccacctg taatgtcatg ctgttcatcg tggttaatgc 180
tgctgtgtgc tgtgtgtgtg tgttgtttgg cgctcattgt tgcgttatgc agcgtacacc 240
acaatattgg aagcttatta gcctttctat tttttcgttt gcaaggctta acaacattgc 300
tgtggagagg gatggggata tggaggccgc tggagggagt cggagaggcg ttttggagcg 360
gcttggcctg gcgcccagct cgcgaaacgc acctaggacc ctttggcacg ccgaaatgtg 420
ccacttttca gtctagtaac gccttaccta cgtcattcca tgcatgcatg tttgcgcctt 480
ttttcccttg cccttgatcg ccacacagta cagtgcactg tacagtggag gttttggggg 540
ggtcttagat gggagctaaa agcggcctag cggtacacta gtgggattgt atggagtggc 600
atggagccta ggtggagcct gacaggacgc acgaccggct agcccgtgac agacgatggg 660
tggctcctgt tgtccaccgc gtacaaatgt ttgggccaaa gtcttgtcag ccttgcttgc 720
gaacctaatt cccaattttg tcacttcgca cccccattga tcgagcccta acccctgccc 780
atcaggcaat ccaattaagc tcgcattgtc tgccttgttt agtttggctc ctgcccgttt 840
cggcgtccac ttgcacaaac acaaacaagc attatatata aggctcgtct ctccctccca 900
accacactca cttttttgcc cgtcttccct tgctaacaca aaagtcaaga acacaaacaa 960
ccaccccaac ccccttacac acaagacata tctacagca 999
<210> 161
<211> 412
<212> DNA
<213> Saccharomyces cerevisiae
<400> 161
atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg actccgcgca 60
tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc tttcttcctc 120
tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga ccgcctcgtt 180
tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt tcttgaaaat 240
tttttttttt gatttttttc tctttcgatg acctcccatt gatatttaag ttaataaacg 300
gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact ttttttactt 360
cttgctcatt agaaagaaag catagcaatc taatctaagt tttaattaca aa 412
<210> 162
<211> 406
<212> DNA
<213> Yarrowia lipolytica
<400> 162
agagaccggg ttggcggcgc atttgtgtcc caaaaaacag ccccaattgc cccaattgac 60
cccaaattga cccagtagcg ggcccaaccc cggcgagagc ccccttctcc ccacatatca 120
aacctccccc ggttcccaca cttgccgtta agggcgtagg gtactgcagt ctggaatcta 180
cgcttgttca gactttgtac tagtttcttt gtctggccat ccgggtaacc catgccggac 240
gcaaaataga ctactgaaaa tttttttgct ttgtggttgg gactttagcc aagggtataa 300
aagaccaccg tccccgaatt acctttcctc ttcttttctc tctctccttg tcaactcaca 360
cccgaaatcg ttaagcattt ccttctgagt ataagaatca ttcaaa 406
<210> 163
<211> 205
<212> DNA
<213> Saccharomyces cerevisiae
<400> 163
gttaattcaa attaattgat atagtttttt aatgagtatt gaatctgttt agaaataatg 60
gaatattatt tttatttatt tatttatatt attggtcggc tcttttcttc tgaaggtcaa 120
tgacaaaatg atatgaagga aataatgatt tctaaaattt tacaacgtaa gatattttta 180
caaaagccta gctcatcttt tgtca 205
<210> 164
<211> 300
<212> DNA
<213> Yarrowia lipolytica
<400> 164
gcgtctacaa ctggaccctt agcctgtata tatcaattga ttatttaaag atttggtcgg 60
taggcggttc gtattgtaca atgggatctg ttactgaggt ggatctaccc aacttgcgag 120
attcaattgc gagattcaat cgcgagattc aattgcgaga atcagttgcg agttgttcta 180
acactcagct tctacgagcg cttgtattag gacgagtgat actccgtggg gcgacggctt 240
ctcttgcgtc ttctgttgta ttctttctta cactatcgtc catctccaac cacctcgtac 300
<210> 165
<211> 300
<212> DNA
<213> Saccharomyces cerevisiae
<400> 165
gcgtctacaa ctggaccctt agcctgtata tatcaattga ttatttaaag atttggtcgg 60
taggcggttc gtattgtaca atgggatctg ttactgaggt ggatctaccc aacttgcgag 120
attcaattgc gagattcaat cgcgagattc aattgcgaga atcagttgcg agttgttcta 180
acactcagct tctacgagcg cttgtattag gacgagtgat actccgtggg gcgacggctt 240
ctcttgcgtc ttctgttgta ttctttctta cactatcgtc catctccaac cacctcgtac 300
<210> 166
<211> 400
<212> DNA
<213> Yarrowia lipolytica
<400> 166
gctgcttgta cctagtgcaa ccccagtttg ttaaaaatta gtagtcaaaa acttctgagt 60
tagaaatttg tgagtgtagt gagattgtag agtatcatgt gtgtccgtaa gtgaagtgtt 120
attgactctt agttagttta tctagtactc gtttagttga cactgatcta gtattttacg 180
aggcgtatga ctttagccaa gtgttgtact tagtcttctc tccaaacatg agagggctct 240
gtcactcagt cggcctatgg gtgagatggc ttggtgagat ctttcgatag tctcgtcaag 300
atggtaggat gatgggggaa tacattactg ctctcgtcaa ggaaaccaca atcagatcac 360
accatcctcc atggtatccg atgactctct tctccacagt 400
Claims (22)
1.一种转化细胞，其包含第一遗传修饰、第二遗传修饰和第三遗传修饰，其中：
所述第一遗传修饰提高所述细胞中磷酸转酮酶蛋白质的活性；
所述第二遗传修饰提高所述细胞中磷酸乙酰转移酶蛋白质的活性；并且
所述第三遗传修饰提高所述细胞中果糖1,6二磷酸酶蛋白质的活性或者降低所述细胞中天然磷酸果糖激酶蛋白质、天然果糖二磷酸醛缩酶蛋白质或天然丙糖磷酸异构酶蛋白质的活性。
2.根据权利要求1所述的转化细胞，其中所述第一遗传修饰是使用第一核酸的转化，并且所述第一核酸编码磷酸转酮酶蛋白质。
3.根据权利要求2所述的转化细胞，其中所述第一核酸编码与SEQ ID NO:21、SEQ IDNO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQID NO:123或SEQ ID NO:125所示的序列或者其中任一个的生物活性部分具有至少95％序列同源性的氨基酸序列。
4.根据权利要求1至3中任一项所述的转化细胞，其中所述第二遗传修饰是使用第二核酸的转化，并且所述第二核酸编码磷酸乙酰转移酶蛋白质。
5.根据权利要求4所述的转化细胞，其中所述第二核酸编码与SEQ ID NO:1、SEQ IDNO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ IDNO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ IDNO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ ID NO:63、SEQ IDNO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ ID NO:75、SEQ IDNO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ ID NO:87、SEQ IDNO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ ID NO:99、SEQ IDNO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119所示的序列或者其中任一个的生物活性部分具有至少95％序列同源性的氨基酸序列。
6.根据权利要求1至5中任一项所述的转化细胞，其中所述第三遗传修饰提高所述细胞中果糖1,6二磷酸酶蛋白质的活性，所述第三修饰是使用第三核酸的转化，并且所述第三核酸编码果糖1,6二磷酸酶蛋白质。
7.根据权利要求1至5中任一项所述的转化细胞，其中所述第三遗传修饰降低所述细胞中天然磷酸果糖激酶蛋白质、天然果糖二磷酸醛缩酶蛋白质或天然丙糖磷酸异构酶蛋白质的活性，并且所述第三遗传修饰是敲除突变。
8.根据权利要求1至7中任一项所述的转化细胞，其还包含选自由以下所组成的组的第四遗传修饰：
降低所述细胞中天然转醛醇酶蛋白质、天然甘油醛3-磷酸脱氢酶蛋白质、天然磷酸甘油酸激酶蛋白质、天然磷酸甘油酸变位酶蛋白质、天然烯醇化酶蛋白质或天然丙酮酸激酶蛋白质的活性的遗传修饰；以及
提高所述细胞中可溶性转氢酶蛋白质或外部氧化还原酶蛋白质的活性的遗传修饰。
9.根据权利要求8所述的转化细胞，其中：
所述第四遗传修饰降低所述细胞中天然转醛醇酶蛋白质、天然甘油醛3-磷酸脱氢酶蛋白质、天然磷酸甘油酸激酶蛋白质、天然磷酸甘油酸变位酶蛋白质、天然烯醇化酶蛋白质或天然丙酮酸激酶蛋白质的活性；并且
所述第四遗传修饰是敲除突变。
10.根据权利要求8所述的转化细胞，其中：
所述第四遗传修饰提高所述细胞中可溶性转氢酶蛋白质或外部氧化还原酶蛋白质的活性；
并且所述第四遗传修饰是使用编码所述可溶性转氢酶蛋白质或外部氧化还原酶蛋白质的核酸的转化。
11.根据权利要求1至10中任一项所述的转化细胞，其中所述细胞选自由藻类、细菌、霉菌、真菌、植物和酵母组成的组。
12.根据权利要求11所述的转化细胞，其中所述细胞选自由以下组成的组：Arxula酵母属(Arxula)、曲霉属(Aspegillus)、裂殖壶菌属(Aurantiochytrium)、假丝酵母属(Candida)、麦角菌属(Claviceps)、隐球菌属(Cryptococcus)、小克银汉霉属(Cunninghamella)、地霉属(Geotrichum)、汉逊酵母属(Hansenula)、克鲁维酵母属(Kluyveromyces)、柯达酵母属(Kodamaea)、白冬孢酵母属(Leucosporidiella)、油脂酵母属(Lipomyces)、被孢霉属(Mortierella)、Ogataea酵母属(Ogataea)、毕赤酵母属(Pichia)、原壁菌属(Prototheca)、根霉属(Rhizopus)、红冬孢酵母属(Rhodosporidium)、红酵母属(Rhodotorula)、酵母属(Saccharomyces)、裂殖酵母属(Schizosaccharomyces)、银耳属(Tremella)、毛孢子菌属(Trichosporon)、威克汉姆酵母属(Wickerhamomyces)和耶氏酵母属(Yarrowia)。
13.根据权利要求12所述的转化细胞，其中所述细胞选自由以下组成的组：Arxula酵母(Arxula adeninivorans)、黑曲霉(Aspergillus niger)、米曲霉(Aspergillus orzyae)、土曲霉(Aspergillus terreus)、裂殖壶菌(Aurantiochytrium limacinum)、产朊假丝酵母(Candida utilis)、麦角菌(Claviceps purpurea)、白色隐球菌(Cryptococcus albidus)、弯曲隐球菌(Cryptococcus curvatus)、新型隐球菌(Cryptococcus ramirezgomezianus)、地生隐球酵母(Cryptococcus terreus)、威氏隐球菌(Cryptococcus wieringae)、刺孢小克银汉霉(Cunninghamella echinulata)、山茶小克银汉霉(Cunninghamella japonica)、发酵地霉(Geotrichum fermentans)、多形汉逊酵母(Hansenula polymorpha)、乳酸克鲁维酵母(Kluyveromyces lactis)、马克思克鲁维酵母(Kluyveromyces marxianus)、奥默柯达菌(Kodamaea ohmeri)、冰岛白冬孢酵母(Leucosporidiella creatinivora)、产油油脂酵母(Lipomyces lipofer)、斯达油脂酵母(Lipomyces starkeyi)、子囊菌油脂酵母(Lipomyces tetrasporus)、深黄被孢霉(Mortierella isabellina)、高山被孢霉(Mortierella alpina)、Ogataea酵母(Ogataea polymorpha)、西弗毕赤酵母(Pichiaciferrii)、季也蒙毕赤酵母(Pichia guilliermondii)、巴斯德毕赤酵母(Pichiapastoris)、树干毕赤酵母(Pichia stipites)、左氏原壁菌(Prototheca zopfii)、无根根霉(Rhizopus arrhizus)、巴布维红冬孢酵母(Rhodosporidium babjevae)、圆红冬孢酵母(Rhodosporidium toruloides)、沼泽生红冬孢酵母(Rhodosporidium paludigenum)、黏红酵母(Rhodotorula glutinis)、胶红酵母(Rhodotorula mucilaginosa)、酿酒酵母(Saccharomyces cerevisiae)、粟酒裂殖酵母(Schizosaccharomyces pombe)、银耳(Tremella enchepala)、皮肤毛孢子菌(Trichosporon cutaneum)、发酵毛孢子菌(Trichosporon fermentans)、威克汉姆西弗酵母(Wickerhamomyces ciferrii)和解脂耶氏酵母(Yarrowia lipolytica)。
14.根据权利要求13所述的转化细胞，其中所述细胞选自由Arxula酵母、酿酒酵母和解脂耶氏酵母组成的组。
15.根据权利要求1至14中任一项所述的转化细胞，其中所述细胞不是酿酒酵母。
16.根据权利要求1至15中任一项所述的转化细胞，其中所述细胞不包含天然丙酮酸脱羧酶基因中的缺失、突变或置换。
17.根据权利要求1至16中任一项所述的转化细胞，其中所述细胞生产选自由以下组成的组的产品：油、脂质、脂肪酸、脂肪醇、三酰基甘油酯、类异戊二烯或法呢烯。
18.根据权利要求1至17中任一项所述的转化细胞，其中所述细胞包含与相同类型的未经修饰细胞相比更高的脂质含量。
19.一种提高细胞的脂质含量的方法，其包括：
用编码磷酸转酮酶蛋白质的第一核苷酸序列转化所述细胞；
用编码磷酸乙酰转移酶蛋白质的第二核苷酸序列转化所述细胞；以及
用第三核苷酸序列转化所述细胞，所述第三核苷酸序列编码果糖1,6二磷酸酶蛋白质或者降低所述细胞中天然磷酸果糖激酶蛋白质、天然果糖二磷酸醛缩酶蛋白质或天然丙糖磷酸异构酶蛋白质的活性。
20.根据权利要求19所述的方法，其中所述第一核苷酸序列编码与SEQ ID NO:21、SEQID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ ID NO:29、SEQ ID NO:31、SEQ ID NO:121、SEQ ID NO:123或SEQ ID NO:125所示的序列或者其中任一个的生物活性部分具有至少95％序列同源性的氨基酸序列。
21.根据权利要求19或20所述的方法，其中所述第二核苷酸序列编码与SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37SEQ ID NO:39、SEQ ID NO:41、SEQ ID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51、SEQ ID NO:53、SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59、SEQ ID NO:61、SEQ IDNO:63、SEQ ID NO:65、SEQ ID NO:67、SEQ ID NO:69、SEQ ID NO:71、SEQ ID NO:73、SEQ IDNO:75、SEQ ID NO:77、SEQ ID NO:79、SEQ ID NO:81、SEQ ID NO:83、SEQ ID NO:85、SEQ IDNO:87、SEQ ID NO:89、SEQ ID NO:91、SEQ ID NO:93、SEQ ID NO:95、SEQ ID NO:97、SEQ IDNO:99、SEQ ID NO:101、SEQ ID NO:103、SEQ ID NO:105、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:113、SEQ ID NO:115、SEQ ID NO:117或SEQ ID NO:119所示的序列或者其中任一个的生物活性部分具有至少95％序列同源性的氨基酸序列。
22.根据权利要求19至21中任一项所述的方法，其还包括用第四核苷酸序列转化所述细胞，其中：
所述第四核苷酸序列降低所述细胞中天然转醛醇酶蛋白质、天然甘油醛3-磷酸脱氢酶蛋白质、天然磷酸甘油酸激酶蛋白质、天然磷酸甘油酸变位酶蛋白质、天然烯醇化酶蛋白质或天然丙酮酸激酶蛋白质的活性；或者
所述第四核苷酸序列提高所述细胞中可溶性转氢酶蛋白质或外部氧化还原酶蛋白质的活性。
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| CN108950050A (zh) * | 2018-08-15 | 2018-12-07 | 江西省农业科学院水稻研究所 | 东野型水稻胞质雄性不育恢复基因Rf（DW）10的检测引物、试剂盒及应用与检测方法 |
| CN110499259A (zh) * | 2019-07-22 | 2019-11-26 | 浙江工业大学 | 一种解酯耶氏酵母yw100-1及其应用 |
| CN110950943A (zh) * | 2019-12-30 | 2020-04-03 | 上海交通大学 | 紫花苜蓿‘WL525’胚胎发育晚期蛋白MsLEA-D34及其编码基因 |
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| AU2015266724B2 (en) | 2014-05-29 | 2021-09-23 | Ginkgo Bioworks, Inc. | Increasing lipid production in oleaginous yeast |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| CN108220324A (zh) * | 2016-12-09 | 2018-06-29 | 中国科学院大连化学物理研究所 | 一种促进产油酵母生产油脂的方法 |
| KR102572191B1 (ko) * | 2018-04-26 | 2023-08-30 | 한국과학기술원 | 지질 축적량이 향상된 변이 균주 |
| US20240200019A1 (en) * | 2021-04-29 | 2024-06-20 | Ginkgo Bioworks, Inc. | Genetically modified yeast cells and methods of use for increased lipid yield |
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| EP3240886A1 (en) | 2017-11-08 |
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