CN107771214A

CN107771214A - 用于具有增加的2,4‑二羟基丁酸外排物的优化的2,4‑二羟基丁酸产生的修饰的微生物

Info

Publication number: CN107771214A
Application number: CN201680033086.5A
Authority: CN
Inventors: W·蒂舍特; L·迪蒙-赛格诺维特; P·瓦瑟尔; G·贝斯泰尔-卡尔; P·索卡耶
Original assignee: Metabolic Explorer SA
Current assignee: Metabolic Explorer SA
Priority date: 2015-04-07
Filing date: 2016-04-07
Publication date: 2018-03-06
Anticipated expiration: 2036-04-07
Also published as: BR112017021306A2; US10858675B2; WO2016162442A1; CN107771214B; US20180105845A1; EP3280794A1; EP3280794B1; ES2811337T3

Abstract

本发明涉及能够产生2,4‑二羟基丁酸的重组微生物，其特征在于所述2,4‑DHB的增加的细胞输出，且优选地在于所述2,4‑DHB的降低的细胞输入。本发明还涉及通过在发酵培养基中培养所述微生物并从所述培养基中回收2,4‑DHB而用于2,4‑二羟基丁酸的优化产生的方法。

Description

用于具有增加的2,4-二羟基丁酸外排物的优化的2,4-二羟基丁酸产生的修饰的微生物

引言

本发明涉及能够产生2,4-二羟基丁酸的重组微生物，其特征在于所述2,4-DHB的增加的细胞输出，并且优选地在于所述2,4-DHB的降低的细胞输入。本发明还涉及通过在发酵培养基中培养所述微生物并从所述培养基中回收2,4-DHB而用于2,4-二羟基丁酸的优化产生的方法。

2,4-二羟基丁酸(2,4-dihydroxybutyric acid)(即2,4-DHB或DHB)，也称为2,4-二羟基丁酸(2,4-dihydroxybutanoic acid)、2,4-二羟基丁酸(2,4-dihydroxybutyrate)或3-脱氧-L-甘油-特窗酸(3-deoxy-L-glycero-tetronic acid)，是高经济利益的工业化学化合物，因为其可用作多种大宗化学品和精细化学品合成的前体，其中有甲硫氨酸类似物2-羟基-4-(甲基硫代)丁酸酯/盐(HMTB)和2-酮基-4(甲基硫代)丁酸酯/盐(KMTB)(两者均以每年大约800,000吨生产)、γ-丁内酯(GBL)(大约500,000吨/年)以及许多其他生物技术产品(超过大约1,000,000吨/年)。特别是，HMTB是众所周知的作为低价氨基酸替代物广泛用于动物营养的食品添加剂(US2009/318715)，同时已知GBL是用于清洁电路板、剥漆、调味豆制品或甚至用于生产γ-羟基丁酸(GHB)药物的有机溶剂。

2,4-二羟基丁酸可通过使甘油(glyceritol)转化为丙烯醛，接着水合所产生的2-脱氧甘油糖(3-羟基丙醛)，随后使用氰化物处理以产生腈，然后将其水解并使用番木鳖碱(brucine)处理以分离L-对映体2,4-DHB来产生。然而，这种DHB的石油化学合成由于它依赖危险材料和条件的使用在经济上不是切实可行的，耗时并且昂贵，并且，直到今天，没有鉴定出用于其生化产品的天然代谢途径。

因此，在本领域中需要通过备选的有成本效益的方法产生这种高度相关的化合物，其会降低对石油原料的依赖。

合成代谢途径看起来特别引人注意，因为它们提供了绿色且可持续的方法以更低成本产生2,4-DHB。事实上，最近已开发了依赖于微生物中特定酶的重组表达的多种代谢工程方法：WO2012/056318、WO2013/160762、WO2014/009435和EP14306564.7描述了经由不同代谢途径通过在遗传修饰微生物中的葡萄糖发酵而产生2,4-DHB。通过基于作用于空间上相似的同族底物的候选酶的结构和机械知识的合理工程化，或通过筛选天然酶并通过合理设计进一步改善来获得这些专利申请中鉴定的大多数酶。更具体地，WO2012/056318公开了三种非天然存在的酶(苹果酸激酶、苹果酸半醛脱氢酶和DHB脱氢酶，均被突变)，其可在微生物中过表达以将代谢中间产物(L)-苹果酸转化为2,4-DHB；WO2013/160762需要多种酶的异源表达，它们中的一些经突变以改善酶活性和/或底物亲和力(苹果酰CoA合成酶和/或琥珀酰CoA:(L)-苹果酸CoA转移酶和/或苹果酰CoA裂合酶；苹果酰CoA还原酶；和DHB脱氢酶)以使代谢中间产物苹果酸或琥珀酰CoA或羟乙酰CoA转化为2,4-DHB；而WO2014/009435的方法依赖于使代谢中间产物(L)-高丝氨酸变为2-氧代-4-羟基丁酸(OHB)的转化和通过重组表达两种经突变的酶(高丝氨酸转氨酶和DHB还原酶)来还原2,4-DHB中的OHB。近来，METabolic EXplorer公司设计了用于在包括使1,2,4-丁三醇氧化为2,4-二羟基丁醛，然后使2,4-二羟基丁醛氧化为2,4-DHB的两个单独步骤中从1,2,4-丁三醇代谢中间产物微生物产生2,4-二羟基丁酸的替代合成途径(专利申请尚未公布)。

然而。这些不同的方法需要进一步改善。实际上，如上所述的经遗传修饰的微生物通过发酵以有限量产生2,4-DHB，而且与所需工业规模一致的更高生产率和/或产率可受限于微生物细胞内2,4-DHB的积累。

本发明解决了上述讨论的本领域的需求。

特别是，本发明的发明人令人惊奇且意想不到的发现，可通过遗传工程改造微生物以降低细胞内2,4-DHB积累而大大改善2,4-DHB的总体生产，而无论所选择的2,4-DHB生产代谢途径。更具体地，这种积累可通过如下降低：遗传上改善感兴趣的微生物的2,4-DHB细胞输出能力以及遗传上弱化其从培养基中所含的2,4-DHB的细胞内输入。

因此，本发明在本文中提供了用于2,4-二羟基丁酸的优化产生的经遗传修饰的微生物。这种经工程改造以产生2,4-DHB的微生物经进一步遗传修饰用于降低细胞内2,4-二羟基丁酸积累，从而优化其产生。

本发明还涉及通过发酵用于2,4-二羟基丁酸的优化产生的方法，其包括在培养基中培养本发明的微生物，以及从所述培养基中回收产生的2,4-DHB。

发明详述

应该理解的是，下面的详细描述不是限制性的，并且可在不背离本发明的范围的情况下进行各种修改、替换、省略和改变。还应该理解，本文使用的术语是为了描述本发明的具体实施方案的目的，而并不意图为限制性的。

除非另有说明，与本发明相关使用的科学和技术术语应具有由本领域普通技术人员通常理解的含义。此外，除非上下文另有要求，否则本文所使用的命名和分子生物学技术、细胞培养技术是公知的和本领域中通常使用的那些。此类技术在文献(见Sambrook等,2012)中充分地解释。

本文所引用的所有出版物、专利和专利申请(无论是上文还是下文)通过提述以其整体由此并入。

尽管如此，就贯穿本说明书的不同术语的使用而言，下述定义更特别适用。

除非上下文另有明确说明，单数形式“一种”、“一个”和“该/所述”在本文中包括复数指称。因此，例如，提及“微生物”包括多种此类微生物，并且提及“内源基因”是提及一个或多个内源基因，等等。

术语“包含”、“含有”、“涉及”或“包括”或变型如“包含(comprises)”、“包含(comprising)”、“含有”、“涉及”、“包括(includes)”、“包括(including)”在本文中以包括在内的含义使用，即详明所述特征的存在，但是不排除在本发明的多种实施方案中其他特征的存在或添加。

如本文所使用的术语“微生物”指活的微观生物体，其可为单细胞或多细胞生物，并通常可在自然界中找到。在本发明的上下文中，微生物优选是细菌、酵母或真菌。更优选地，本发明的微生物选自肠杆菌科(Enterobacteriaceae)、芽孢杆菌科(Bacillaceae)、梭菌科(Clostridiaceae)、链霉菌科(Streptomycetaceae)、棒杆菌科(Corynebacteriaceae)和酵母。甚至更优选地，本发明的微生物是埃希氏菌属(Escherichia)、克雷伯氏菌属(Klebsiella)、热厌氧杆菌属(Thermoanaerobacterium)、梭菌属(Clostridium)、棒杆菌属(Corynebacterium)或酵母属的菌种。还甚至更优选地，本发明的微生物选自大肠杆菌(Escherichia coli)、肺炎克雷伯氏菌(Klebsiella pneumoniae)、热解糖热厌氧杆菌(Thermoanaerobacterium thermosaccharolyticum)、丙酮丁醇梭菌(Clostridiumacetobutylicum)、谷氨酸棒杆菌(Corynebacterium glutamicum)和酿酒酵母(Saccharomyces cerevisiae)。最优选地，本发明的微生物是大肠杆菌。

如本文所用，术语“重组微生物”、“经遗传修饰的微生物”或“遗传工程化微生物”指如上文定义的微生物，所述微生物在自然界中找不到，因此在遗传上与其天然对应物不同。换言之，它是指通过导入和/或通过缺失和/或通过修饰其遗传元件而经修饰的微生物。此类修饰可通过基因工程、通过在特定选择压力下培养微生物而迫使新代谢途径的形成和进化、或通过组合这两种方法进行(参见例如WO2005/073364或WO2008/116852)。

因此，根据本发明的经遗传修饰用于产生2,4-DHB的微生物意指所述微生物是能够产生2,4-DHB的如上文定义的重组微生物。换言之，所述微生物已经经遗传修饰而允许产生2,4-DHB。

如以下进一步解释的，本发明的微生物可通过调节一个或多个内源基因的表达水平，和/或通过在所述微生物中表达一个或多个异源基因而被遗传修饰。

“基因”在本文中意指编码特定蛋白质(即多肽)的核酸分子或多核苷酸，或在某些情况下，编码功能性或结构性RNA分子。在本发明的上下文中，本文所提及的基因编码蛋白质，如酶、外排系统(efflux system)或摄取转运蛋白(uptake transporter)。根据本发明的基因是内源的基因或外源的。“内源基因”在本文中意指所述基因天然存在于微生物中，而术语“外源基因”(或可替换的“异源基因”或“转基因”)指不天然存在于微生物中的基因。

在本发明的上下文中，微生物可经遗传修饰以“调节”一个或多个内源基因的表达水平，本文中意指所述基因的表达水平与其天然表达水平相比被上调、下调(即弱化)，或甚至完全取消。因此所述调节可导致基因产物的活性增强，或者可替换地，导致内源基因产物的较低活性或无活性。

可通过引入除内源调控元件外有利于上调的异源序列或通过用此类异源序列替换那些内源调控元件，或者通过将内源基因的一个或多个补充拷贝引入微生物内的染色体或质粒来过表达内源基因。也可通过将突变引入其编码序列中以修饰基因产物来修饰内源基因活性和/或表达水平。还可进行内源基因的缺失以完全抑制其在微生物内的表达。调节内源基因表达的另一种方式是用更强或更弱的启动子交换其启动子(即野生型启动子)以上调或下调此基因的表达水平。适于此类目的的启动子可为同源的或异源的并且是本领域中公知的。选择适当的启动子来调节内源基因的表达在本领域技术人员的能力范围内。。

另外/或者，本发明的微生物可经遗传修饰而表达一个或多个外源基因，条件是将所述基因与对于其在宿主微生物中表达必需的所有调节元件一起导入微生物中。用外源DNA修饰或“转化”微生物是本领域技术人员的常规任务。在本发明的上下文中，术语“过表达”在本文中也就外源基因在微生物中的表达而言使用。

为了在微生物中表达外源基因，可将此类基因直接整合入微生物染色体中，或者通过微生物内的质粒或载体在染色体外表达。多种质粒(在其复制起点和其在细胞中的拷贝数方面不同)在本领域中是公知的，并可由熟练技术人员容易地选择用于此目的。根据本发明的外源基因有利地为同源基因。

在本发明的上下文中，术语“同源基因”或“同源物”不仅指通过理论上共同的遗传祖先由两个物种(即微生物物种)遗传的基因，而且包括可为遗传上无关但已进化以编码进行相似功能和/或具有相似结构的蛋白质的基因(即功能同源物)。因此，术语“功能同源物”在本文中指编码功能同源蛋白质的基因。

使用数据库，如Uniprot(用于蛋白质)、Genbank(用于基因)或NCBI(用于蛋白质或基因)中可用的信息，本领域技术人员可容易地确定微生物的特定蛋白质和/或基因的序列，并基于此序列鉴定另一种微生物中的等同基因或同源物之一。此常规工作可通过一种微生物的特定基因序列与其它微生物的基因序列或基因组(其可在上文提及的数据库中找到)的序列比对来进行。有利地，可使用Altschul等(1990)开发的BLAST算法进行此类序列比对。一旦在那些序列之间建立了序列同源性，可得出共有序列并用于设计简并探针以克隆相关微生物的相应同源物基因。分子生物学的这些常规方法是本领域技术人员公知的。

应当进一步理解的是，在本发明的上下文中，如果在特定的微生物中表达编码感兴趣的蛋白质的外源基因，则优选通过将非优选的密码子或不太优选的密码子替换为编码相同氨基酸的所述微生物的优选密码子来构建此基因的合成形式。本领域确实公知密码子选择在微生物物种之间变化，这可影响感兴趣的蛋白质的重组表达水平。为了克服此问题，已开发了密码子优化方法，并由Graf等(2000),Deml等(2001)或Davis&Olsen(2011)广泛描述。已开发出几个软件用于密码子优化测定，如软件(Lifetechnologies)或OptimumGene^TM软件(GenScript)。换言之，编码感兴趣蛋白质的外源基因优选进行密码子优化用于在特定微生物中表达。

根据本发明的微生物还可经遗传修饰以增加或减少一个或多个蛋白质的活性。

可通过改善蛋白质催化效率、通过降低蛋白质周转率(turnover)、通过降低信使RNA(mRNA)周转率、通过增加基因的转录、或通过增加mRNA的翻译获得增加此类活性。

改善蛋白质催化效率意指对于给定底物和/或给定辅因子，增加kcat和/或降低Km，和/或对于给定抑制剂，增加Ki。Kcat、Km和Ki是本领域技术人员能够确定的Michaelis-Menten常数(Segel，1993)。减少蛋白质周转率意指稳定蛋白质。改善蛋白质催化效率和/或降低蛋白质周转率的方法是本领域技术人员公知的。那些包括合理的工程化改造及序列和/或结构分析和定向诱变，以及随机诱变和筛选。可通过常规方法如聚合酶链式反应(PCR)进行定点诱变，通过随机诱变技术例如经由诱变剂(紫外线或化学试剂如亚硝基胍(NTG)或乙基甲磺酸(EMS))或DNA改组或易错PCR引入突变。稳定蛋白质也可通过在蛋白质的N-末端或C-末端添加“标签”肽序列来实现。此类标签在本领域中是公知的，并且包括谷胱甘肽-S-转移酶(GST)等等。

可通过修饰5’-非翻译区(5’-UTR)和/或编码区和/或3’-UTR的基因序列减少mRNA周转率(Carrier和Keasling，1999)。

增加基因的转录(无论是内源的还是外源的)可通过增加微生物内其拷贝的数目和/或通过使用与野生型启动子相比导致更高的基因表达水平的启动子来实现。在本发明的上下文中，术语“过表达”也用于指微生物中基因转录的增加。

如上文指示，为了增加微生物中基因的拷贝数，可在染色体上或在染色体外编码所述基因。当要在染色体上编码感兴趣的基因时，可通过本领域公知的遗传重组方法(例如基因置换)在染色体上引入基因的几个拷贝。当要在微生物中在染色体外编码基因时，它可由不同类型的质粒携带，所述质粒就其复制起点而言取决于它们可在其中复制的微生物以及它们在细胞中的拷贝数而不同。根据质粒的性质，由所述质粒转化的微生物可含有质粒的1至5个拷贝，或其约20个拷贝，或甚至其多达500个拷贝。可在大肠杆菌中复制的低拷贝数质粒的实例包括但不限于pSC101质粒(紧密复制)、RK2质粒(紧密复制)以及pACYC和pRSF1010质粒，而可在大肠杆菌中复制的高拷贝数质粒的例子是pSK bluescript II。

可增加基因表达水平的启动子也是本领域技术人员公知的，并且可为同源的(起源于相同的物种)或异源的(起源于不同的物种)。此类启动子的实例包括但不限于启动子Ptrc、Ptac、Plac和λ启动子cI。这些启动子也可由特定的化合物或由特定的外部条件如温度或光诱导(“诱导型启动子”)。

可通过修饰核糖体结合位点(RBS)增加mRNA的翻译。RBS是启动蛋白质翻译时与核糖体结合的mRNA上的序列。它可为真核生物中mRNA的5’帽、原核生物中起始密码子AUG上游6-7个核苷酸区(称为Shine-Dalgarno序列)、或病毒中的内部核糖体进入位点(IRES)。通过修饰此序列，可改变蛋白质翻译起始速率，按比例改变其生产速率，和控制其在细胞内的活性。也可通过使用软件RBS CALCULATOR(Salis，2011)来优化RBS序列的强度以达到靶向的翻译起始速率。基于mRNA的性质选择RBS序列在本领域技术人员的能力范围内。

降低蛋白质的活性可意指通过突变编码所述蛋白质的基因来降低其特异的催化活性，从而通过突变核苷酸序列或通过缺失所述基因的编码区来改变相应的氨基酸序列和/或降低细胞中蛋白质的浓度。

如本文所使用的术语“外排系统”、“外排泵”、“外排转运蛋白”或“输出蛋白(exporter)”指以能量依赖方式从细胞的细胞质和/或周质输出底物分子的蛋白质组装(protein assembly)。因此，外排系统通常位于细胞的细胞质膜中(跨越细胞质膜)。值得注意地，在革兰氏阴性细菌(如大肠杆菌)中，外排系统可跨越周质空间并且还可存在外排系统的部分，其跨越外膜。细菌外排系统是本领域公知的，值得注意地例如通过由加利福尼亚大学圣地亚哥分校的Milton H.Saier Jr.所述的转运机制的分类系统(网址:http://www.tcdb.org/)，并且通常被归为使用三磷酸腺苷(ATP)作为能量来源的初级主动转运蛋白(primary active transporter)，或归为次级主动转运蛋白(secondary activetransporter)，其中转运通过转运的底物的电化学势能差(单向转运蛋白(uniporter))或通过偶联于第二底物(例如氢离子或钠离子)从细胞外部或向细胞外部的转运(逆向转运蛋白(antiporter)和同向转运蛋白(symporter))来驱动。基于它们的氨基酸序列和用来输出它们的底物的能量来源，迄今已鉴定了细菌外排转运蛋白的五个超家族，并包括主要易化蛋白超家族(major facilitator superfamily)(MFS)、ATP结合盒超家族(ATP-bindingcassette superfamily)(ABC)、小分子多药抗性家族(the small multidrug resistancefamily)(SMR)、抗性结节细胞分裂超家族(the resistance-nodulation-cell divisionsuperfamily)(RND)和多抗微生物挤压蛋白家族(the Multi antimicrobial extrusionprotein family)(MATE)。在本发明的上下文中，外排系统从微生物生长于其中的培养基中的细胞的细胞质和/或周质输出2,4-DHB作为底物。直到今天，所述输出2,4-DHB的外排系统仍然未知。本发明的一个目的是鉴定能够输出2,4-DHB的外排系统。候选外排系统从细胞输出2,4-DHB的能力可通过在体外测量与不表达此系统的对照细胞相比从天然表达或过表达所述外排系统的细胞产生的细胞外2,4-DHB的量来评估。这还可通过在细胞中测量2,4-DHB的胞内浓度来完成。输出的抑制可通过测量此外排系统的已知抑制剂降低从细胞输出2,4-DHB或增加细胞内2,4-DHB的浓度的能力来进一步评价。为此，本领域的技术人员可根据文献和专利申请(例如Kutukova等,2005；Hiller等,2007；Kiefer等,2007；Bolten等,2007；Zittrich&1994；Dassler等,2000；WO2005/085463；EP1239041)中已广泛描述的用于测试多种底物的外排系统的提取和代谢分析方法的评估改变方案。根据本发明的合适的外排系统的实例在下文，特别是表1中进一步描述。

“摄取转运蛋白”、“摄取系统”、“摄取泵”或“摄取搬运蛋白(uptake porter)”在本文中意指将底物分子以能量依赖或非依赖方式(主动或被动摄取)输入至细胞的细胞质和/或周质的蛋白质组装。类似于外排系统，摄取转运蛋白可位于细胞的细胞质和/或周质膜。细菌摄取转运蛋白是本领域中众所周知的，并且例如通过加利福尼亚大学圣地亚哥分校的Milton H.Saier Jr.分类(网址:http://www.tcdb.org/)。在本发明的上下文中，摄取转运蛋白将2,4-DHB作为底物从微生物生长于其中的培养基输入至所述微生物细胞的细胞质和/或周质。直到今天，所述输出2,4-DHB的外排系统仍然未知。本发明的一个目的是鉴定能够输入2,4-DHB的摄取转运蛋白。候选摄取转运蛋白将2,4-DHB输入细胞的能力可通过测量与不表达此转运蛋白的对照细胞相比在天然表达或过表达所述转运蛋白的细胞中所含细胞内2,4-DHB的量来评估。输入的抑制可通过测量此转运蛋白的已知抑制剂降低2,4-DHB摄取入细胞的能力来进一步评价。这样做，本领域的技术人员可改变已在文献和专利申请(Kurihara等,2009；Hiller等,2007；Kiefer等,2007；Bolten等,2007；WO2014029592)中广泛描述的用于测试多种底物的摄取转运蛋白的方案。根据本发明的合适的摄取转运蛋白的实例在下文，特别是表1中进一步描述。

一般来说，2,4-DHB特异性的外排和/或摄取系统的遗传修饰的相关性通过改善所述分子的产生和/或通过对大量所述分子的抗性来监测。在后者中，测量重组菌株的生长速率并将其看作细胞内部的2,4-DHB浓度降低的标志。

“编码外排系统或摄取转运蛋白的基因”，在本文中意编码所述系统或转运蛋白的多核苷酸或核酸序列。在编码外排系统的外源基因过表达的情况下，本领域的技术人员会容易地识别插入的多核苷酸序列不需要是相同的，并且可为与其来源的基因的序列“基本上相同”。实际上，由于密码子简并性，一些多核苷酸序列会编码相同蛋白质。此外，如上所释，编码感兴趣的蛋白质的外源基因优选地经密码子优化用于在特定微生物中表达。所述定义作必要的修正适用于本发明的编码其他蛋白质的基因。

“功能变体”，在本文中意指结构上不同于参考蛋白质的氨基酸序列但是通常保留所述参考蛋白质的所有必要功能特征的蛋白质。蛋白质的变体可为天然存在的变体或者非天然存在的变体。参考蛋白质的所述非天然存在的变体可例如通过编码核酸或基因的诱变技术，例如通过随机诱变或定点诱变来制成。

结构差异可以以参考蛋白质的氨基酸序列和变体的氨基酸序列总体上可为非常相似且在许多区中相同的方式受限制。结构差异可由参考蛋白质和变体的氨基酸序列之间的保守或非保守氨基酸取代、缺失和/或添加而产生。唯一的条件是，即使一些氨基酸被取代、缺失和/或添加，参考蛋白质的氨基酸序列的生物活性仍由变体保留。也就是说，在本发明的上下文中，外排系统的变体能够从微生物的细胞输出2,4-DHB，而摄取转运蛋白的变体能够将2,4-DHB输入微生物的细胞。显示所述活性的变体的能力可根据上述体外测试评估。然而必须注意的是与参考的外排系统或摄取转运蛋白的氨基酸序列的活性相比，所述变体的活性可在其2,4-DHB输出或输入效率方面不同。

根据本发明的外排系统或摄取转运蛋白的“功能变体”包括但不限于在与编码所述外排系统或摄取转运蛋白的氨基酸序列比对后具有至少80％同一的氨基酸序列的蛋白质。优选地，所述变体与所述外排系统或摄取转运蛋白具有85％、90％、95％序列同一性，并且更优选与所述外排系统或摄取转运蛋白具有96％、97％、98％、99％或99.999％序列同一性。

氨基酸序列之间的序列同一性可通过比较各序列中的位置来确定，所述序列可为了比较的目的对齐。当比较序列中的位置被相同氨基酸占据时，则序列在那个位置相同。蛋白质之间的序列同一性的程度是由所述蛋白质序列共享的位置处的相同氨基酸残基数目的函数。

为了确定两个氨基酸序列之间的同一性百分比，对齐序列以进行最佳比较。例如，可将缺口(gap)引入第一氨基酸序列的序列中以与第二氨基酸序列进行最佳比对。然后比较在相应氨基酸位置处的氨基酸残基。当第一序列中的位置被与第二序列中相应位置相同的氨基酸残基占据时，分子在那个位置是相同的。

两个序列之间的同一性百分比是由序列共享的相同位置的数目的函数。因此％同一性＝相同位置的数目/重叠位置的总数X 100。

序列的最佳比对可通过Needleman和Wunsch(1972)的全局同源比对算法、通过此算法的计算机化执行或通过肉眼检查来进行。选择通过各种方法产生的最佳比对(即，在比较的序列之间得到最高的同一性百分比)。

换言之，序列同一性的百分比通过如下计算：比较两个最佳对齐序列，确定在两个序列中存在相同氨基酸的位置的数目以获得匹配位置数目，用匹配位置数目除以位置总数，并将所得结果乘以100以获得序列同一性的百分比。

术语“碳水化合物”在本文中指任何能够通过微生物代谢的碳源，并且含有至少一个碳原子，两个氢原子和一个氧原子。本发明的碳水化合物优选选自下组：单糖如葡萄糖、果糖、甘露糖、半乳糖等，二糖如蔗糖、纤维二糖、麦芽糖、乳糖等，寡糖如棉子糖、水苏糖、麦芽糖糊精等，多糖如纤维素、半纤维素、淀粉等，甲醇，甲醛和甘油。根据本发明的优选实施方式，碳源有利的是包含3、6或12个碳原子的碳水化合物，或它们的任何组合。在本发明的更优选的实施方式中，碳源选自木糖、甘油、葡萄糖、半乳糖、果糖、乳糖、麦芽糖、蔗糖，以及它们的任何组合。

降低细胞内2,4-DHB积累

在第一方面，本发明涉及经遗传修饰用于通过发酵产生2,4-二羟基丁酸的微生物，其中所述微生物经进一步遗传修饰用于降低细胞内2,4-二羟基丁酸积累，由此优化2,4-二羟基丁酸产生。

根据本发明的优选实施方式，用于降低细胞内2,4-二羟基丁酸积累的遗传修饰是：

i)过表达至少一个编码外排系统的基因；和/或

ii)缺失至少一个编码摄取转运蛋白的基因或弱化其表达。

要过表达的外排系统更优选地选自下组：单羧酸外排系统、甲酸外排系统、乳酸外排系统、苹果酸外排系统、琥珀酸外排系统、芳族羧酸外排系统、它们的功能变体，以及它们的任何组合。

更优选地，所述外排系统选自下组：

○氨基酸序列SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7、SEQ IDNO:9、SEQ ID NO:11、SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:17和SEQ ID NO:19的单羧酸外排系统，

○氨基酸序列SEQ ID NO:21的甲酸外排系统，

○氨基酸序列SEQ ID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ ID NO:29、SEQID NO:31、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37、SEQ ID NO:39、SEQ ID NO:41、SEQID NO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51和SEQ ID NO:53的乳酸外排系统，

○氨基酸序列SEQ ID NO:55、SEQ ID NO:57、SEQ ID NO:59和SEQ ID NO:61的苹果酸外排系统，

○氨基酸序列SEQ ID NO:63、SEQ ID NO:65和SEQ ID NO:67的琥珀酸外排系统，

○氨基酸序列SEQ ID NO:69和SEQ ID NO:71的芳族羧酸外排系统，

○它们的功能变体，其与所述氨基酸序列具有至少80％序列同一性，以及

○它们的任何组合。

甚至更优选地，所述外排系统选自下组：

○氨基酸序列SEQ ID NO:1的单羧酸外排系统，

○氨基酸序列SEQ ID NO:23的乳酸外排系统，

○氨基酸序列SEQ ID NO:25的乳酸外排系统，

○它们的任何组合。

要弱化或缺失的摄取转运蛋白更优选地选自下组：α-酮戊二酸摄取转运蛋白、乳酸摄取转运蛋白、乙醇酸摄取转运蛋白、乙酸摄取转运蛋白、丙酸摄取转运蛋白、泛酸摄取转运蛋白、琥珀酸和乙酸摄取转运蛋白、乙酰乙酸摄取转运蛋白、葡糖酸摄取转运蛋白，它们的功能变体，和它们的任何组合。

更优选地，所述摄取转运蛋白选自下组：

○氨基酸序列SEQ ID NO:73的α-酮戊二酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:75的乳酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:77的乙醇酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:79和SEQ ID NO:81的乙酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:83的丙酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:85的泛酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:87的琥珀酸和乙酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:89的乙酰乙酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:91的葡糖酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:93的摄取转运蛋白，

○它们的任何组合。

甚至更优选地，所述摄取转运蛋白选自下组：

○氨基酸序列SEQ ID NO:73的α-酮戊二酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:75的乳酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:77的乙醇酸摄取转运蛋白，

○它们的任何组合。

上述提及的外排系统和摄取转运蛋白是本领域中众所周知的，并且下表1和实施例2和3中描述。

表1：本发明的用于降低2,4-DHB细胞内积累的蛋白质和基因(NA＝不可用；*：本领域中已知的底物，用于通过所述外排系统从细胞的细胞质和/或周质输出，或者通过所述摄取转运蛋白输入细胞的细胞质和/或周质)

产生2,4-DHB的代谢途径

如上所示，根据本发明的微生物经遗传修饰产生2,4-DHB。工程改造所述微生物在本领域技术人员的能力范围内。实际上，根据2,4-DHB可能衍生自的感兴趣的代谢中间产物，在本领域中，特别是在专利申请WO2012/056318、WO2013/160762和WO2014/009435(全部通过提述并入本文)中已经描述了在微生物中产生2,4-DHB的多种遗传工程代谢途径。所述代谢中间产物的实例包括但不限于1,2,4-丁三醇、(L)-苹果酸、(L)苹果酰CoA和(L)-高丝氨酸。

由此本发明的优选实施方式提供了微生物，其中通过如上所述的遗传修饰降低细胞内2,4-二羟基丁酸积累，并且其还经遗传修饰将以下代谢中间产物的至少一个转化为2,4-二羟基丁酸：

iii)1,2,4-丁三醇；

iv)(L)-苹果酸；

v)(L)苹果酰CoA；和

vi)(L)-高丝氨酸。

本领域的技术人员会容易理解的是，与降低如上所述的细胞内2,4-二羟基丁酸积累有关的优选实施方式可与以下进一步描述的与2,4-DHB产生有关的优选实施方式组合。

通过1,2,4-丁三醇途径产生2,4-二羟基丁酸

用于从1,2,4-丁三醇中间代谢产物微生物产生2,4-二羟基丁酸的可能合成途径可在两个单独步骤中进行，所述步骤需要将1,2,4-丁三醇连续氧化成2,4-二羟基丁醛，然后将2,4-二羟基丁醛氧化成2,4-DHB。

优选地，根据以下修饰，如上所述的本发明的微生物经遗传修饰将1,2,4-丁三醇转化为2,4-二羟基丁酸：

○过表达至少一个编码作用于供体的CH-OH基的氧化还原酶的多核苷酸，由此将1,2,4-丁三醇转化为2,4-二羟基丁醛；和

○过表达至少一个作用于供体的醛基或氧代基团的氧化还原酶的多核苷酸，由此将2,4-二羟基丁醛转化为2,4-二羟基丁酸。

作用于供体的CH-OH基的氧化还原酶的实例包括，但不限于，EC 1.1.1酶(以NAD+或NADP+作为受体的氧化还原酶，也称为NAD+/NADP+氧化还原酶)、EC 1.1.2酶(以细胞色素作为受体的氧化还原酶)、EC 1.1.3酶(以氧作为受体的氧化还原酶)、EC 1.1.4酶(以二硫化物作为受体的氧化还原酶)、EC 1.1.5酶(以醌或类似化合物作为受体的氧化还原酶)、EC1.1.98酶(具有其他已知受体的氧化还原酶)和EC 1.1.99酶(具有其他受体的氧化还原酶)。

作用于醛基或氧代基团的氧化还原酶的实例包括，但不限于，EC 1.2.1酶(以NAD+或NADP+作为受体的氧化还原酶，也称为NAD+/NADP+氧化还原酶)、EC 1.2.2酶(以细胞色素作为受体的氧化还原酶)、EC 1.2.3酶(以氧作为受体的氧化还原酶)、EC 1.2.4酶(以二硫化物作为受体的氧化还原酶)、EC 1.2.5酶(以醌或类似化合物作为受体的氧化还原酶)、EC1.2.7酶(以铁硫蛋白作为受体的氧化还原酶)和EC 1.2.99酶(具有其他受体的氧化还原酶)。

显示上述活性的酶是本领域中众所周知的，并且可容易地由本领域的技术人员鉴定，例如来自公众可获得的数据库如BRENDA。

为了将1,2,4-丁三醇氧化为2,4-二羟基丁醛，作用于供体的CH-OH基的氧化还原酶(EC 1.1酶)优选选自下组：醇脱氢酶(或醛还原酶)、乳醛还原酶、乙醛酸还原酶、二脱氢葡糖酸还原酶，以及它们的任何组合。更具体地，所述EC 1.1氧化还原酶是醇脱氢酶(或醛还原酶)或乳醛还原酶。还甚至更优选地，所述EC 1.1氧化还原酶是作用于供体的CH-OH基的NAD+/NADP+氧化还原酶(即EC 1.1.1酶)，或具有其它受体的作用于供体的CH-OH基的氧化还原酶(即EC 1.1.99酶)。最优选地，所述EC 1.1酶是EC 1.1.1酶。选择适合于本发明目的的EC 1.1酶并鉴定它们相应的基因(即核苷酸)序列在本领域技术人员的能力范围内。

特别优选的作用于供体的CH-OH基的氧化还原酶(EC 1.1酶)在下表2中描述：醇脱氢酶包括，但不限于，氨基酸序列SEQ ID NO:95至SEQ ID NO:134的酶；乳醛还原酶包括，但不限于，氨基酸序列SEQ ID NO:135的酶；乙醛酸还原酶包括，但不限于，氨基酸序列SEQ IDNO:136和SEQ ID NO:137的酶，和二脱氢葡糖酸还原酶包括，但不限于，氨基酸序列SEQ IDNO:138和SEQ ID NO:139的酶。

在本发明一个特别优选的实施方式中，作用于供体的CH-OH基的氧化还原酶(EC1.1酶)是氨基酸序列SEQ ID NO:130或SEQ ID NO:135的酶。

如上所示，为了将2,4-二羟基丁醛氧化为2,4-二羟基丁酸，本发明的微生物经遗传修饰从而使至少一个编码作用于供体的醛基或氧代基团的氧化还原酶(EC 1.2酶)的基因过表达。

因此，作用于供体的醛基或氧代基团的氧化还原酶(EC 1.2酶)优选选自下组：醛脱氢酶、醛氧化酶以及它们的任何组合。更优选地，所述EC 1.2氧化还原酶是醛脱氢酶。还甚至更优选地，所述EC 1.2氧化还原酶是作用于供体的醛基或氧代基团的NAD+/NADP+氧化还原酶(即EC 1.2.1酶)，或以氧作为受体作用于供体的醛基或氧代基团的氧化还原酶(即EC 1.2.3酶)。最优选地，所述EC 1.2酶是EC 1.2.1酶。选择适合于本发明目的的EC 1.2酶并鉴定它们相应的基因(即核苷酸)序列在本领域技术人员的能力范围内。

特别优选的作用于供体的醛基或氧代基团的氧化还原酶(EC 1.2酶)在下表2中描述：醛脱氢酶包括，但不限于，氨基酸序列SEQ ID NO:140至SEQ ID NO:154的酶；醛氧化酶包括，但不限于，氨基酸序列SEQ ID NO:155的酶。

在本发明一个特别优选的实施方式中，作用于供体的醛基或氧代基团的氧化还原酶(EC 1.2酶)是氨基酸序列SEQ ID NO:140、SEQ ID NO:148或SEQ ID NO:149的酶。

如本领域的技术人员众所周知的，中间代谢产物1,2,4-丁三醇可由微生物通过发酵作为碳源的木糖而产生。如通过提述并入本文的专利申请EP15305096.8、WO2008/091288和US2013/0203141所述，这种转化可通过进一步对微生物进行遗传工程改造来实现。

因此，微生物优选地经进一步遗传修饰以将木糖转化为1,2,4-丁三醇，所述遗传修饰是至少一个以下基因的过表达：

○编码木糖脱氢酶的基因，

○编码木糖酸内酯酶的基因，

○编码木糖酸脱氢酶的基因，

○编码3-脱氧-D-甘油-戊酮糖酸(DGP)脱羧酶的基因，

○编码1,2,4-丁三醇脱氢酶的基因，和

○它们的任何组合，

所述酶是本领域中众所周知的，其中优选的一些在下表2和实施例1(途径1)中描述。

但是，根据优选的实施方式，本发明的微生物经进一步遗传修饰用于从除木糖外的碳源提供2,4-二羟基丁酸产生和微生物生长的还原能力和/或能量，和/或至少部分地(优选完全地)抑制碳分解代谢物阻遏。

所述遗传修饰是特别有利的，由于它们通过首先使用可替换的碳源而非木糖提供还原能力和/或能量，从而木糖可被排他地转化为2,4-二羟基丁酸；和其次通过降低在可能喜好一些碳源超过其他碳源的微生物中观察到的碳分解代谢物阻遏，来优化2,4-DHB产生。值得注意地，大多数天然存在的微生物(包括大肠杆菌)相对于其他糖更喜欢使用葡萄糖，即使它们能够代谢一系列单糖(Kim等,2010)。因此本文优选的是抑制微生物中的分解代谢物阻遏，所述微生物不能以有效方式共利用木糖和另外的糖。

因此，为了从除木糖外的碳源提供用于2,4-二羟基丁酸产生和微生物生长的还原能力和/或能量，根据本发明的微生物优选通过缺失和/或弱化至少一个选自下组的基因而进行遗传修饰：

○编码木糖异构酶的基因，

○编码木酮糖激酶的基因，

○编码3-脱氧-D-甘油-戊酮糖酸醛缩酶的基因，

○编码酮酸脱氢酶的基因，和

○它们的任何组合。

所述酶是本领域中众所周知的，并且在通过提述并入本文的专利申请US2013/0203141中描述。这些酶以使用木糖或产生可与木糖转化为2,4-二羟基丁酸竞争的碳通量的其他代谢产物而特别知名。根据本发明的用于提供所述还原能力和/或能量的优选的酶在下表2和实施例1(途径1)中描述。

仍然，在一个优选的实施方式中，允许抑制碳分解代谢物阻遏的遗传修饰选自以下的至少一个：

○缺失编码磷酸转移酶系统的葡萄糖通透酶的基因，

○缺失编码磷酸载体Hpr蛋白的基因，

○优选地从不受cAMP-CRP调控的组成型启动子或诱导型启动子表达涉及糖输入系统的基因和/或操纵子，其中所述糖是除木糖外的碳源，

○优选地从不受cAMP-CRP调控的组成型启动子或诱导型启动子表达编码木糖转运蛋白(如同向转运蛋白或ABC转运蛋白)的基因，

○过表达编码葡萄糖同向转运蛋白的基因，

○过表达编码葡萄糖易化蛋白的基因，

○过表达编码葡萄糖激酶的基因，

○调节涉及cAMP水平的基因，优选编码腺苷酸环化酶的基因的表达，

○调节编码CRP和/或CRP样蛋白的基因的表达，

○优选从不受cAMP-CRP调控的组成型启动子或诱导型启动子表达编码cAMP非依赖性CRP蛋白的基因，以及

○它们的任何组合。

更优选地，为了共利用木糖和葡萄糖，缺失编码磷酸转移酶和/或磷酸载体Hpr蛋白的基因有利地与过表达编码葡萄糖通透酶或葡萄糖易化蛋白的基因连同过表达编码葡萄糖激酶的基因组合。

根据进一步优选的实施方式，本发明的微生物包含至少一个涉及产生NADPH作为还原能力来源的基因的另外的遗传修饰。实际上，还原酶(如脱氢酶)需要微生物中可用的(特别是NADPH形式的)还原能力。增加细胞中NADPH的可用性的策略是本领域中众所周知的，并且值得注意的是已经由Lee等(2013)综述，并且还由通过提述并入本文的US8088620、WO2012/055798和EP14305691.9描述。

根据本发明，用于改善NADPH产生以及由此改善其在微生物中的可用性的遗传修饰优选选自：

○过表达编码膜结合转氢酶的基因，

○缺失或弱化编码可溶性转氢酶的基因，

○过表达编码NADPH生成甘油醛3-磷酸脱氢酶的基因，

○缺失或弱化编码磷酸葡萄糖异构酶的基因，

○缺失或弱化编码磷酸果糖激酶的基因，

○过表达编码葡萄糖-6-磷酸脱氢酶的基因，

○过表达编码能够生成NADPH的硫辛酰胺脱氢酶的突变体基因，

○过表达编码双功能NAD(P)H水合物修复酶的基因，以及

○它们的任何组合。

缺失或弱化编码磷酸果糖激酶的基因更优选与过表达编码葡萄糖-6-磷酸脱氢酶的基因组合，以通过戊糖磷酸途径增加NADPH的通量。

更优选地，用于改善NADPH产生的遗传修饰选自：

○过表达编码膜结合转氢酶的基因，

○缺失或弱化编码磷酸葡萄糖异构酶和/或可溶性转氢酶的基因，以及

○过表达编码NADPH生成甘油醛3-磷酸脱氢酶的基因。

通过(L)-苹果酸途径产生2,4-二羟基丁酸

用于微生物产生2,4-二羟基丁酸的备选的合成途径使用(L)-苹果酸作为代谢中间产物。工程改造能够将(L)-苹果酸转化为2,4-DHB的微生物在本领域技术人员的能力范围内。实际上，遗传修饰用于将(L)-苹果酸转化为2,4-DHB的微生物和用于工程改造所述微生物的方法是本领域中众所周知的，并且已经在通过提述并入本文的专利申请WO2012/056318中特别描述。

因此，根据以下修饰，本发明的微生物经遗传修饰用于将(L)-苹果酸转化为2,4-二羟基丁酸：

○过表达至少一个编码苹果酸激酶的基因，由此将苹果酸转化为4-磷酸苹果酸；

○过表达至少一个编码苹果酸半醛脱氢酶的基因，由此将4-磷酸苹果酸转化为苹果酸-4-半醛；以及

○过表达至少一个编码DHB脱氢酶的基因，由此将苹果酸-4-半醛转化为2,4-二羟基丁酸。

所述酶是本领域中众所周知的，其中优选的一些在下表2和实施例1(途径3)中描述。

优选地，所述苹果酸激酶为氨基酸序列SEQ ID NO:198的，所述苹果酸半醛脱氢酶为氨基酸序列SEQ ID NO:199的，并且所述DHB脱氢酶为氨基酸序列SEQ ID NO:200的。

通过(L)苹果酰CoA途径产生2,4-二羟基丁酸

用于微生物产生2,4-二羟基丁酸的另一个备选的合成途径使用(L)苹果酰CoA作为代谢中间产物。工程改造能够将(L)苹果酰CoA转化为2,4-DHB的微生物在本领域技术人员的能力范围内。实际上，经遗传修饰用于将(L)苹果酰CoA转化为2,4-DHB的微生物和工程改造所述微生物的方法是本领域中众所周知的，并且已经在通过提述并入本文的专利申请WO2013/160762中特别描述。

因此，根据以下修饰，本发明的微生物经遗传修饰用于将(L)苹果酰CoA转化为2,4-二羟基丁酸：

○过表达至少一个编码苹果酰CoA还原酶的基因，由此将苹果酰CoA转化为苹果酸-4-半醛；以及

还优选地，所述微生物经进一步遗传修饰以：

○通过过表达至少一个编码苹果酰CoA合成酶的基因将(L)苹果酸转化为(L)苹果酰CoA；或

○通过过表达至少一个编码琥珀酸CoA:(L)苹果酰CoA转移酶的基因将琥珀酰CoA转化为(L)苹果酰CoA；或

○通过过表达至少一个编码苹果酰CoA裂合酶的基因，将乙醛酸转化为(L)苹果酰CoA。

所述酶是本领域中众所周知的，其中优选的一些在下表2和实施例1(途径4)中描述。

优选地，所述苹果酰CoA还原酶为氨基酸序列SEQ ID NO:201的，所述所述DHB脱氢酶为氨基酸序列SEQ ID NO:200的，并且所述苹果酰CoA裂合酶为氨基酸序列SEQ ID NO:202的，

进一步优选的遗传修饰在以下实施例1(途径4)中描述。

通过(L)-高丝氨酸途径产生2,4-二羟基丁酸

仍然，用于微生物产生2,4-二羟基丁酸的另一个合成途径使用(L)-高丝氨酸作为代谢中间产物。工程改造能够将(L)-高丝氨酸转化为2,4-DHB的微生物在本领域技术人员的能力范围内。实际上，经遗传修饰用于将(L)-高丝氨酸转化为2,4-DHB的微生物和工程改造所述微生物的方法是本领域中众所周知的，并且已经在通过提述并入本文的专利申请WO2014/009435中或在专利申请EP14306564.7(尚未公布)中特别描述。

因此，根据以下修饰，本发明的微生物经遗传修饰用于将(L)-高丝氨酸转化为2,4-二羟基丁酸：

○过表达至少一个编码选自下组的酶的基因：高丝氨酸氧化酶、高丝氨酸脱氢酶、高丝氨酸转氨酶和它们的任何组合，由此将(L)-高丝氨酸转化为2-氧代-4-羟基丁酸；以及

○过表达至少一个编码2-氧代-4-羟基丁酸(OHB)还原酶的基因，由此将2-氧代-4-羟基丁酸(OHB)转化为2,4-二羟基丁酸。

所述酶是本领域中众所周知的，其中优选的一些在下表2和实施例1(途径2)中描述。

优选地，所述高丝氨酸脱氢酶为氨基酸序列SEQ ID NO:203的，并且所述2-氧代-4-羟基丁酸(OHB)还原酶为氨基酸序列SEQ ID NO:204的。

进一步优选的遗传修饰在以下实施例1(途径2)中描述。

表2：本发明用于产生2,4-DHB的蛋白质和基因

(NA＝不可用；ND＝未公开)

用于产生2,4-DHB的方法

在另一方面，本发明涉及用于产生2,4-二羟基丁酸的方法，其包括：

a)在使所述碳源转化为2,4-二羟基丁酸的发酵条件下，在包含碳源的培养基中培养如上所述的经遗传修饰的微生物，以及

b)从所述培养基中回收2,4-二羟基丁酸。

发酵培养基和碳源是本领域中公知的。根据本发明，术语“发酵过程”、“发酵”或“培养”可互换使用，指允许给定微生物生长的实验条件。微生物的生长通常在具有适于所用微生物的适当生长培养基的发酵罐中进行。

“适当的培养基”在本文中指培养基(例如无菌液体培养基)，其包含对于微生物的维持和/或生长必需的或有益的营养物如碳源或碳底物；氮源，例如蛋白胨、酵母提取物、肉提取物、麦芽提取物、尿素、硫酸铵、氯化铵、硝酸铵和磷酸铵；磷源，例如磷酸二氢钾或磷酸氢二钾；微量元素(例如金属盐)，例如镁盐、钴盐和/或锰盐；以及生长因子如氨基酸和维生素。

在本发明的一个优选的实施方式中，碳源，优选碳水化合物，来源于可再生的原料，如植物生物质。

本领域的技术人员可容易地确定培养根据本发明的微生物必需的培养条件。具体地，公知细菌可在20℃-55℃，优选25℃-40℃的温度发酵。大肠杆菌更具体地可在约30℃-约37℃的温度培养。

本发明的方法可以分批过程、补料分批过程或连续过程并在需氧、微需氧或厌氧条件下进行。

“在需氧条件下”的发酵意指通过将气体溶解到培养物的液相中来将氧气提供给培养物。这可通过如下实现：(1)将含氧气体(例如空气)喷入液相中，或者(2)摇动含有培养基的容器以将顶部空间中所含的氧气转移到液相中。在需氧条件下发酵的主要优点是氧作为电子受体的存在改善菌株产生更多ATP形式的能量用于细胞过程的能力，由此改善菌株的一般代谢。

微需氧条件可在本文中使用并且定义为将低百分比的氧气(例如，使用含有0.1-10％的氧气，用氮气填充至100％的气体混合物)溶解到液相中的培养条件。

相反，“厌氧条件”定义为不向培养基提供氧气的培养条件。严格厌氧条件可通过向培养基中喷入惰性气体(如氮气)来除去痕量的其它气体来获得。硝酸盐可用作电子受体以改善菌株的ATP产生并改善其代谢。

根据一个优选的实施方式，本发明的方法进一步包括纯化步骤b)的2,4-DHB的步骤c)。

用于纯化羧酸和具体地羟基酸的方法在本领域中是公知的，并且特别在WO2002/090312、WO2002/022544和WO2012/153042中描述，其通过提述并入本文。2,4-DHB的纯化可在发酵完成后或在发酵过程中通过包括提取发酵的原位产物回收来进行(Van Hecke等,2014)。

在任一情况下，可通过经过发生固/液分离的设备，优选通过具有20至200kDa的截留范围的过滤器来除去微生物。还可行的是采用离心机、适合的沉积装置或这些设备的组合，尤其优选的是通过沉积首先分离至少一部分微生物并且随后将已经部分地释放了微生物的发酵液进料至超滤或离心装置。

在除去微生物后，包含在所得发酵溶液中的2,4-DHB可通过用氢氧化钙沉淀接着用硫酸重新酸化来纯化，然而此过程产生大量之后需要消除的硫酸钙(Schügerl,2000)。或者，可通过将例如铵化合物添加至发酵液以产生2,4-DHB的铵盐来沉淀2,4-DHB。然后此铵盐可通过加入有机提取剂并随后加热所得混合物而从发酵溶液中除去，由此铵盐在有机相中浓缩。然后可例如通过进一步的提取步骤从该相中分离2,4-DHB以给出纯的2,4-DHB(WO2002/090312)。

在无需任何进一步纯化或提取步骤的更简单的过程中，可通过使用活性炭或功能化树脂，但以相对低效的方式(Husson&King 1999)来纯化2,4-DHB。基于相同的相互作用原理，可通过离子交换或疏水层析来纯化2,4-DHB。或者，可通过电渗析、反渗透、超滤或纳滤来纯化2,4-DHB(Cho等,2012)。在那种情况下，将2,4-DHB作为水溶液回收。

在提取发酵的情况下，可使用例如胺化合物，如三正癸胺(Gao等,2009)通过液-液萃取从发酵液中纯化2,4-DHB。或者，可通过例如与醇如丁醇酯化的反应性蒸馏来回收2,4-DHB(Rao等,2014)。

根据以下实施例会更好地理解本发明，其仅被提供用于说明目的。然而，本领域技术人员会容易地理解的是，这些详细的实施例是非限制性的，并且在不脱离本发明的范围的情况下可做出各种修改、替换、省略和改变。

附图简述

图1表示用于2,4-DHB产生的4种代谢途径(*：任选地弱化或缺失的基因)。

图2表示用于将D-木糖转化为2,4-DHB的代谢途径(*：任选地弱化或缺失的基因)。

实施例

在以下实施例中进一步限定了本发明。应该理解的是，这些实施例虽然指示了本发明的优选实施方案，但仅以说明的方式给出。从上面的公开和这些实例中，本领域的技术人员可对本发明进行各种变化以使其适于各种用途和条件，而不需要修改本发明的基本手段。

描述了构建具有这些能力的微生物所需要的示例性基因和酶以及用于克隆和转化、监测产物形成和使用工程化微生物进行生产的方法。

具体地，实施例显示了经修饰的大肠杆菌菌株，但是这些修饰可容易地在相同科的其它微生物中进行。

大肠杆菌属于肠杆菌科，其包含革兰氏阴性、杆状、非孢子形成，并且长度通常为1-5μm的成员。大多数成员具有用于活动的鞭毛，但一些属是不能运动的。此科的许多成员是在人类和其它动物的肠中发现的肠道菌群的正常部分，而其它在水中或土壤中发现，或者是多种不同动物和植物上的寄生物。大肠杆菌是最重要的模式生物体之一，但肠杆菌科的其它重要成员包括克雷伯氏菌属(特别是土生克雷伯氏菌、植物克雷伯氏菌或产酸克雷伯氏菌)，泛菌属和沙门氏菌属。

方案

以下实施例中描述了用于构建2,4-二羟基丁酸生产菌株的几种方案。

在本发明中使用的方案1(通过同源重组的染色体修饰、重组体的选择和抗生素盒切除)和方案2(噬菌体P1的转导)已经在专利申请WO2013/001055中充分描述。

方案3：构建重组质粒

本领域技术人员已知充分描述和知道重组DNA技术。

简言之，使用寡核苷酸(本领域技术人员能够设计)和MG1655大肠杆菌K-12或其它微生物基因组DNA作为基质(根据待扩增的靶向基因)对DNA片段进行PCR扩增。用相容的限制酶消化DNA片段和选择的质粒，连接，然后转化到感受态细胞中。分析转化体并通过DNA测序验证感兴趣的重组质粒。

实施例1：在MG1655大肠杆菌中从4种不同代谢途径构建2,4-DHB产生菌株–图1–构建菌株1至7

途径1：在MG1655大肠杆菌中通过从新途径过量产生酶而从木糖产生2,4-DHB–构建菌株1至4。

修饰大肠杆菌菌株MG1655以使用图2所示的途径从D-木糖产生2,4-二羟基丁酸(2,4-DHB)。这项工作寻求使朝向2,4-DHB生产的碳通量最大化，从而除去所有涉及其它木糖消耗途径或涉及2,4-DHB-中间体化合物的转化(其代表了产物的损失)的酶。

除由大肠杆菌天然表达的基因(序列SEQ ID NO:158的yjhG基因和序列SEQ IDNO:159的yagF基因，其编码木糖酸脱水酶，以及序列SEQ ID NO:160的adhP基因和序列SEQID NO:161的yqhD基因，其编码醇脱氢酶–NAD(P)H依赖性1,2,4-丁三醇脱氢酶)外，使用pCL1920质粒(Lerner&Inouye,1990)，用Ptrc人工启动子(专利WO 2007/0770441中给出的序列)及其自身的核糖体结合位点分别表达编码以下酶的基因：新月柄杆菌的木糖脱氢酶和木糖酸内酯酶(分别为xdh[CauloCyc上的CC0821,SEQ ID NO:156]和xylC[CauloCyc上的CC0820,SEQ ID NO:157])，恶臭假单胞菌的3-脱氧-D-甘油-戊酮糖酸脱羧酶(序列SEQ IDNO:162的mdlC基因)，大肠杆菌或丁酸梭菌的醇脱氢酶–NAD(P)+依赖性2,4-二羟基-丁醛脱氢酶(分别为来自大肠杆菌的编码序列SEQ ID NO:135的酶的fucO基因或来自丁酸梭菌的编码序列SEQ ID NO:130的酶的dhaT基因)，和大肠杆菌的醛脱氢酶(来自大肠杆菌的编码序列SEQ ID NO:140的酶的puuC基因或来自大肠杆菌的编码序列SEQ ID NO:149的酶的aldB基因)的基因。实际上，首先将基因xdh、xylC、mdlC克隆在pCL1920质粒上，产生质粒pDHB0001，然后将fucO或dhaT和puuC或aldB顺序克隆在质粒pDHB0001上，产生4种组合，得到质粒pDHB0002至pDHB0005，如下表所述。

此外，为了阻断天然木糖分解代谢途径，使用由Datsenko&Wanner，2000所述的同源重组策略并且根据方案1，从大肠杆菌MG1655染色体中缺失编码D-木糖异构酶(序列SEQID NO:163的xylA基因)和D-木酮糖激酶(序列SEQ ID NO:164的xylB基因)的基因。更准确地，为了缺失xylAB操纵子，用SEQ ID NO:205和SEQ ID NO:206的引物产生携带抗生素抗性基因连同FRT位点的被与xylAB操纵子的上游和下游区同源的序列围绕的PCR产物，并将其导入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。

为了避免3-脱氧-D甘油戊酮糖酸(DGP)的降解，也使用相同同源重组策略缺失编码酮酸脱氢酶的基因(序列SEQ ID NO:167的yiaE基因；和序列SEQ ID NO:168的ycdW基因)和DGP醛缩酶的基因(序列SEQ ID NO:165的yjhH基因；和序列SEQ ID NO:166的yagE基因)。更准确地，为了缺失yjhH基因(SEQ ID NO:165)，用SEQ ID NO:207和SEQ ID NO:208的引物产生携带抗生素抗性基因连同FRT位点的被与yjhH基因的上游和下游区同源的序列围绕的PCR产物，并将其导入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。为了缺失yagE基因(SEQ ID NO:166)，用SEQ ID NO:209和SEQ ID NO:210的引物产生携带抗生素抗性基因连同FRT位点的被与yagE基因的上游和下游区同源的序列围绕的PCR产物，并将其导入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。为了缺失yiaE基因(SEQ IDNO:167)，使用SEQ ID NO:211和SEQ ID NO:212的引物产生携带抗生素抗性基因连同FRT位点的被与yiaE基因的上游和下游区同源的序列围绕的PCR产物，并将其导入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。为了缺失ycdW基因(SEQ ID NO:168)，使用SEQID NO:213和SEQ ID NO:214的引物产生携带抗生素抗性基因连同FRT位点的被与ycdW基因的上游和下游区同源的序列围绕的PCR产物，并将其导入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。

由于yjhH基因连同yjhG(SEQ ID NO:158)和yjhI属于yjhIHG操纵子，必须选择与yjhH的上游和下游区同源的序列，从而不改变周围基因的表达。与属于yagEF操纵子的yagE基因相同。本领域的技术人员知晓如何从操纵子中除去DNA序列而同时保持操纵子其余部分的开读框。

为了抑制分解代谢物阻遏，通过使用由Datsenko&Wanner,2000(根据方案1)所述的同源重组策略如专利申请EP 14305691.9中，特别是在所述文件的实施例2中(在本文中称为SEQ ID NO:215和SEQ ID NO:216)所述缺失由ptsG基因(SEQ ID NO:169)编码的葡萄糖磷酸转移酶IIBC(Glc)。将适当的PCR产物引入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。

每次，使用卡那霉素(kanamycin)、氯霉素(chloramphenicol)、庆大霉素(gentamycin)、四环素(tetracycline)、杀稻瘟素(blasticidin)或壮观霉素(spectinomycin)中的不同抗生素抗性基因。在使用大肠杆菌MG1655优化菌株之前，如Datsenko&Wanner,2000所述(根据方案1)使用Flp重组酶从ΔxylAB、ΔyjhH、ΔyagE、ΔyiaE、ΔycdW和ΔptsG修饰中除去抗生素盒。

通过增加产生菌株的NADPH可用性来改善2,4-DHB酸产生

由adhP和yqhD编码的1,2,4-丁三醇脱氢酶需要生物体内可用的，特别是NADPH形式的还原能力，因此将涉及NADPH产生的基因过表达。

如专利申请EP 14305691.9中，特别是在所述文件的实施例4中(在本文称为SEQID NO:217)所述通过用诱导型Ptrc启动子(来自质粒pTRC99A，Amersham Pharmacia)和确定的核糖体结合位点RBS120(来自RBS Calculator软件)替代大肠杆菌MG1655的pntA基因的内源启动子和核糖体结合位点而过量产生由pntAB操纵子(SEQ ID NO:188)编码的膜结合质子易位吡啶核苷酸转氢酶。产生专利申请EP 14305691.9中所述的适当的PCR产物，并且将其引入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。

通过使用由Datsenko&Wanner,2000所述(根据方案1)的同源重组策略，和如在专利申请WO 2012/055798中，特别是在所述申请的实施例2中(本文称为SEQ ID NO:218和SEQID NO:219)所述来缺失由sthA基因(以前称为udhA,并且具有序列SEQ ID NO:189)编码的可溶吡啶核苷酸转氢酶。产生适当的PCR产物，并且将其引入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。

通过使用由Datsenko&Wanner,2000所述(根据方案1)的同源重组策略，和如在专利申请EP 14305691.9中，特别是在所述文件的实施例5中(本文称为SEQ ID NO:220和SEQID NO:221)所述来缺失由pfkA基因(SEQ ID NO:193)编码的磷酸果糖激酶。产生适当的PCR产物，并且将其引入先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。

每次，使用卡那霉素、氯霉素、庆大霉素、四环素、杀稻瘟素或壮观霉素中不同的抗生素抗性基因。

在使用大肠杆菌MG1655优化菌株之前，根据方案1使用Flp重组酶从pntAB、sthA和pfkA基因座中除去抗生素盒。

最终，将上述每个质粒pDHB0002、pDHB0003、pDHB0004或pDHB0005引入不同的源于E.coli MG1655的突变体菌株。

所构建菌株的非排他性实例列于表3中。

表3.

途径2：在MG1655大肠杆菌中通过过量产生高丝氨酸转氨酶和2-氧代-4-羟基丁酸还原酶而从高丝氨酸产生2,4-DHB–构建菌株5。

高丝氨酸转化为2,4-DHB的合成途径在具有增强的高丝氨酸产生的大肠杆菌菌株MG1655菌株中表达。

为了改善高丝氨酸产生，编码具有对苏氨酸的反馈抗性降低的大肠杆菌的双功能天冬氨酸激酶/高丝氨酸脱氢酶的突变等位基因(具有将318位中的苯丙氨酸氨基酸变为丝氨酸的点突变的序列SEQ ID NO:222的thrA*1基因)和根瘤菌(Rhizobium etli)的编码丙酮酸羧化酶的基因(序列SEQ ID NO:223的pycre基因)分别从质粒pCL1920(Lerner&Inouye,1990)过表达(不在操纵子中)。更准确地，thrA*1基因从由lacI基因编码的抑制子LacI调控的IPTG诱导型trc启动子(SEQ ID NO:224)过表达，两者均获自质粒pTRC99A(Stratagene)，并且pycre基因从gapA基因的启动子(SEQ ID NO:225)过表达，给出pME101-thrA*1-PgapA-pycre-TT07质粒。更准确地，在本专利申请中的参考文献中添加的专利WO2007/077041中描述了pME101-thrA*1质粒的一个实例。在本专利申请中的参考文献中添加的专利WO2012/055798中描述了获得PgapA-pycre-TT07扩增子并将其克隆入pCL1920载体的一个实例。

为了避免高丝氨酸的降解，在大肠杆菌MG1655染色体上弱化编码高丝氨酸激酶(序列SEQ ID NO:226的thrB基因)、苏氨酸合酶(序列SEQ ID NO:227的thrC基因)和高丝氨酸O-琥珀酰转移酶(序列SEQ ID NO:228的metA基因)的基因。

为了弱化thrBC操纵子的表达，通过使用由Datsenko&Wanner,2000所述(根据方案1)的同源重组策略用人工基因替换操纵子thrBC的天然启动子和thrB基因的核糖体结合位点(RBS)。更准确地，在先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中引入被与染色体上的thrB基因同源的序列和与thrB基因上游区同源的序列(SEQ ID NO:231和SEQID NO:232)围绕的PCR产物，其携带有转录终止子(T7噬菌体的T7Te转录终止子,Harrington等,2001,SEQ ID NO:229)、人工trc启动子和RBS(SEQ ID NO:230)和抗生素抗性基因连同FRT位点。

以相同的方式弱化metA基因。更准确地，在先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中引入被与染色体上的metA基因同源的序列和与metA基因上游区同源的序列(SEQ ID NO:233和SEQ ID NO:234)围绕的PCR产物，其携带有转录终止子(T7噬菌体的T7Te转录终止子,Harrington等,2001,SEQ ID NO:229)、人工trc启动子和RBS(SEQ ID NO:230)和抗生素抗性基因连同FRT位点。

为了将高丝氨酸转化为2,4-DHB，将编码来自大肠杆菌的高丝氨酸转移酶的基因(来自大肠杆菌的编码序列SEQ ID NO:203的酶的ilvE基因)和来自乳酸乳球菌的编码2-氧代-4-羟基丁酸(OHB)还原酶的基因(来自乳酸乳球菌的编码序列SEQ ID NO:204的酶的ldhA基因)在相同操纵子中从获自质粒pEXT20(Dykxhoorn等,1996)的IPTG诱导型tac启动子(SEQ ID NO:235)和获自质粒pET28a(Novagen)的T7RBS(SEQ ID NO:236)过表达入pEXT20载体中，给出pEXT20-DHB质粒。更准确地，在专利WO2014/009435中描述了构建pEXT20-DHB质粒的一个实例。

为了优化2,4-DHB生物合成途径中的碳通量，将编码D-乳酸脱氢酶的基因(来自大肠杆菌的编码序列SEQ ID NO:237的酶的ldhA基因)，编码醇脱氢酶的基因(序列SEQ IDNO:238的adhE基因)和编码丙酮酸激酶的基因(序列SEQ ID NO:239的pykA基因和序列SEQID NO:240的pykF基因)也从大肠杆菌MG1655染色体中缺失。

更准确地，在专利WO2014/009435中描述了在产生菌株中构建基因缺失ΔldhA和ΔadhE并剪切抗性基因的一个实例。在本专利申请中的参考文献中添加的专利WO2009/043803中描述了在产生菌株中构建基因缺失ΔpykA和ΔpykF并剪切抗性基因的一个实例。

为了增加葡萄糖输入至细胞，缺失编码转录双调控子的基因dgsA(或mlc)(序列SEQ ID NO:241的dgsA基因)，其控制编码大肠杆菌磷酸转移酶(PTS)和磷酸烯醇丙酮酸(PEP)系统的酶的多个基因的表达。另一种增加葡萄糖输入至细胞的方式是过量产生PtsG(IICGlc)(来自大肠杆菌的编码序列SEQ ID NO:169的酶的ptsG基因)，葡萄糖磷酸转运系统的跨膜配偶体(transmembrane partner)。在本专利申请中参考文献中添加的专利WO2013/001055中描述了构建基因缺失ΔdgsA、引入产生菌株中和并剪切抗性基因的一个实例。过表达ptsG基因的一个实例是在产生菌株中构建并引入以下质粒pCC1BACVB01-PlacIq-lacI-TT02-Ptrc01/OP01/RBS01*2-ptsG-TT07，如专利WO2013/001055中所述。

根据想要的缺失组合，将每个缺失引入至事先转化有pKD46质粒的构建菌株。每次，使用卡那霉素、氯霉素、庆大霉素、四环素、杀稻瘟素或壮观霉素中不同的抗生素抗性基因。当必要时和在质粒引入前，如Datsenko&Wanner,2000(根据方案1)所述使用Flp重组酶从thrBC、metA、ldhA、adhE、dgsA、pykA或pykF基因座除去抗生素盒。

将不同质粒引入至不同的源于大肠杆菌MG1655的突变体菌株。

所构建菌株的非排他性实例列于表4中。

表4.

途径3：在MG1655大肠杆菌中通过过量产生合成途径的酶(其包括苹果酸激酶、苹果酸半醛脱氢酶和2,4-DHB脱氢酶)而从苹果酸产生2,4-DHB–构建菌株6

苹果酸转化为2,4-DHB的合成途径在大肠杆菌MG1655菌株中表达。

为了将苹果酸转化为2,4-DHB，将来自大肠杆菌的编码苹果酸激酶的lysC的突变的等位基因(来自大肠杆菌的编码序列SEQ ID NO:198的酶的具有将119位中的谷氨酸氨基酸变为甘氨酸和250位中的谷氨酸氨基酸变为赖氨酸的点突变的lysC E119G E250K基因)，来自大肠杆菌的编码苹果酸半醛脱氢酶的asd的突变的等位基因(来自大肠杆菌的编码序列SEQ ID NO:199的酶的具有将241位中的谷氨酸氨基酸变为谷氨酰胺的点突变的asdE241Q基因)和来自勤奋金属球菌的编码DHB脱氢酶的ssr的突变的等位基因(来自勤奋金属球菌的编码序列SEQ ID NO:200的酶的具有使用Geneart(Life Technologie)的软件将39位的组氨酸氨基酸变为精氨酸，将43位的天冬氨酸氨基酸变为组氨酸的点突变和其他沉默突变以优化编码序列用于在大肠杆菌中最大表达的ssr H39R-N43H基因)在相同操纵子中从获自质粒pEXT20(Dykxhoorn等,1996)的IPTG诱导型tac启动子(SEQ ID NO:235)和获自质粒pET28a(Novagen)的T7RBS(SEQ ID NO:236)过表达入pET28a载体中，给出pTAC-DHB质粒。更准确地，在本专利申请中的参考文献中添加的专利WO2012/056318中描述了构建pTAC-DHB质粒的一个实例。

将pTAC-DHB质粒引入至大肠杆菌MG1655菌株，给出菌株27。

所构建菌株的非排他性实例列于表5中。

表5.

菌株	相关基因型
		菌株6	MG1655(pTAC-DHB)

途径4：在MG1655大肠杆菌中通过过量产生合成途径的酶(其包括苹果酰CoA合成酶和/或琥珀酰CoA:(L)-苹果酸CoA转移酶和/或苹果酰CoA裂合酶、苹果酰CoA还原酶和DHB 脱氢酶)而从苹果酸和/或琥珀酰CoA和/或乙醛酸产生2,4-DHB–构建菌株7。

乙醛酸转化为2,4-DHB的合成途径在大肠杆菌MG1655菌株中表达。

为了将乙醛酸转化为2,4-DHB，将编码来自扭脱甲基杆菌的苹果酰CoA裂合酶的基因(来自扭脱甲基杆菌的编码经优化用于在大肠杆菌中表达的序列SEQ ID NO:202的酶的mcl基因)，编码来自东工大硫化叶菌的苹果酰CoA还原酶(丙二酰CoA还原酶)的基因(来自东工大硫化叶菌的编码序列SEQ ID NO:201的酶的具有将206位的酪氨酸变为脯氨酸的点突变的mcr基因)和编码来自勤奋金属球菌的DHB脱氢酶(琥珀酸半醛还原酶)的基因(来自勤奋金属球菌的编码经优化用于在大肠杆菌中表达的序列SEQ ID NO:200的酶的并且具有将39位的组氨酸氨基酸变为精氨酸的点突变，和将43位的天冬氨酸氨基酸变为组氨酸的点突变的ssr基因)在相同操纵子中从获自质粒pACT3(Dykxhoorn等,1996)(其中克隆3个基因的质粒)的IPTG诱导型tac启动子(SEQ ID NO:236)过表达，给出pACT3-MCL-DHB质粒。更准确地，在专利WO2013/160762中描述了构建pACT3-MCL-DHB质粒的一个实例。

为了优化2,4-DHB生物合成途径中的碳通量，并且特别是乙醛酸的可利用性，从大肠杆菌MG1655染色体缺失编码磷酸乙酰转移酶(序列SEQ ID NO:242的pta基因)，苹果酸合酶(序列SEQ ID NO:243的aceB基因)和乙醛酸旁路操纵子的转录调控子(序列SEQ ID NO:244的iclR基因)的基因。

更准确地，在专利WO2013/160762中描述了构建基因缺失Δpta、ΔaceB和ΔiclR、引入产生菌株中并剪切抗性基因的一个实例。

根据想要的缺失组合，将各缺失引入至事先转化有pKD46质粒的构建菌株。每次，使用卡那霉素、氯霉素、庆大霉素、四环素、杀稻瘟素或壮观霉素中不同的抗生素抗性基因。当需要时和在质粒引入之前，如Datsenko&Wanner,2000(根据方案1)所述使用Flp重组酶从pta、aceB或iclR基因座除去抗生素盒。

将质粒pACT3-MCL-DHB引入不同的源于大肠杆菌MG1655的突变体菌株，其经优化用于2,4-DHB产生。

所构建菌株的非排他性实例列于表6中。

表6.

菌株	相关基因型
		菌株7	MG1655ΔptaΔiclRΔaceB(pACT3-MCL-DHB)

实施例2：在MG1655大肠杆菌中通过在来自4种不同代谢途径的2,4-DHB产生菌株中过量产生2,4-DHB输出蛋白而优化2,4-DHB产生–构建菌株8至38

为了促进2,4-DHB输出，并且因此改善2,4-DHB产生，使用pCL1920质粒(Lerner&Inouye,1990)用Ptrc人工启动子(专利WO 2007/0770441中给出的序列)及其自身的核糖体结合位点过表达以下编码输出蛋白的基因之一。过表达的基因是来自大肠杆菌的编码单羧酸MFS(主要易化蛋白超家族(major facilitator superfamily))型转运蛋白的基因(序列SEQ ID NO:2的yhjX基因)，来自酿酒酵母的编码羧酸(乳酸)转运蛋白的基因(序列SEQ IDNO:24的JEN1基因)，来自大肠杆菌的编码内膜代谢产物(乳酸)转运蛋白的基因(序列SEQID NO:26的yhjE基因)和来自粟酒裂殖酵母的编码苹果酸转运蛋白的基因(序列SEQ IDNO:58的mae1基因)。根据在大肠杆菌MG1655修饰菌株中优化的2,4-DHB产生途径，将编码输出蛋白的基因克隆入空的pCL1920质粒或克隆入携带用于2,4-DHB产生的基因并且在上面描述的pCL1920质粒中，(pDHB0002至pDHB0005，加pME101-thrA*1-PgapA-pycre-TT07)。

所得质粒是pDHB0006至pDHB00029，如下表中所述。

将这些质粒分别引入不同的源于大肠杆菌MG1655的突变体菌株(其经优化用于2,4-DHB产生)和野生型MG1655。

所构建菌株的非排他性实例列于表7中。

表7.

实施例3：在MG1655大肠杆菌中通过在来自4种不同代谢途径的2,4-DHB产生菌株中过量产生与缺失2.4-DHB输入蛋白组合的2,4-DHB输出蛋白而优化2,4-DHB产生–构建菌株39至131

为了避免再输入2,4-DHB，并且因此改善2,4-DHB产生，使用由Datsenko&Wanner,2000所述的同源重组策略和根据方案1从大肠杆菌MG1655染色体缺失以下编码输入蛋白的基因之一。缺失的基因是编码α-酮戊二酸通透酶的基因(序列SEQ ID NO:74的kgtP基因)，编码L-乳酸通透酶的基因(序列SEQ ID NO:76的lldP基因)和编码乙醇酸通透酶的基因(序列SEQ ID NO:78的glcA基因)。更准确地，为了缺失kgtP基因，用SEQ ID NO:245和SEQ IDNO:246的引物产生被与kgtP基因的上游和下游区同源的序列围绕的PCR产物，其携带抗生素抗性基因连同FRT位点，并将其引入到先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。为了缺失lldP基因，用SEQ ID NO:247和SEQ ID NO:248的引物产生被与lldP基因的上游和下游区同源的序列围绕的PCR产物，其携带抗生素抗性基因连同FRT位点，并将其引入到先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。为了缺失glcA基因，用SEQ ID NO:249和SEQ ID NO:250的引物产生被与glcA基因的上游和下游区同源的序列围绕的PCR产物，其携带抗生素抗性基因连同FRT位点，并将其引入到先前已经转化有pKD46载体的大肠杆菌MG1655选择菌株中。

每次，在卡那霉素、氯霉素、庆大霉素、四环素、杀稻瘟素或壮观霉素中选择抗生素抗性基因。当需要时和在质粒引入前，如Datsenko&Wanner,2000(根据方案1)所述使用Flp重组酶从kgtP或lldP或glcA基因座除去抗生素盒。

将每个缺失分别引入不同的源于大肠杆菌MG1655的突变体菌株(其经优化用于2,4-DHB产生)和野生型MG1655中。所得菌株列于下表中。

所构建菌株的非排他性实例列于表8中。

表8

实施例4：在培养液中存在大量2,4-DHB的情况下细菌生长和产生2,4-二羟基-丁酸

携带途径1的菌株的培养条件：

使用补充有30g/l MOPS、20g/L D-木糖和10g/l葡萄糖并经调节处于pH6.8的改良M9培养基(Anderson,1946)在500ml带挡板锥形瓶中评价产生菌株。当预培养和培养中需要时，以50mg.L-1的浓度加入壮观霉素。预培养物在37℃在LB培养基(Sigma)中生长。生长24小时后，在30℃和200rpm，将其用于接种改良M9培养基的50mL培养物至OD₆₀₀为约0.2。

携带途径2、3和4的菌株的培养条件：

使用补充有20g.L-1MOPS和20g.L-1葡萄糖并经调节处于pH6.8的改良M9培养基(Anderson,1946)在500ml带挡板锥形瓶中评价产生菌株。当预培养和培养中需要时，以50mg.L-1的浓度加入壮观霉素，以100mg.L-1的浓度加入氨苄青霉素并以25mg.L-1的浓度加入氯霉素。预培养物在37℃在LB培养基(Sigma)中生长。生长24小时后，在30℃和200rpm，将其用于接种改良M9培养基的50mL培养物至OD₆₀₀为约0.2。在培养开始时或当生长培养物的OD₆₀₀达到1时，根据细胞中使用的质粒的拷贝数以100-1000μmol.L-1的浓度加入IPTG。

当培养基中的糖耗尽时，将培养物离心并通过LC-MS/MS分析培养液的2,4-二羟基-丁酸。

2,4-二羟基-丁酸滴度/效价(titer)表示如下：

还监测各菌株的生长速率作为培养基中对高2,4-二羟基-丁酸浓度的耐受性的指示。在这样的情况下，在250ml带挡板的锥形瓶中评价菌株。预培养物在37℃在LB培养基(Sigma)中生长并在24小时后，在30℃和200rpm，将其用于接种补充有10g/L葡萄糖(在菌株携带途径1(菌株1至4；菌株8至23和菌株39至86)的情况下加10g/L木糖)的改良M9培养基的25mL培养物至OD₆₀₀为约0.2。将浓度为10g/L的2,4-DHB(从Sigma Aldrich采购)加入或不加入培养液中，并且生长24小时后，测量并计算OD₆₀₀，比较在两种条件之间各菌株的生长速率。

表9：培养基中10g/L的2,4-DHB对经修饰以减少细胞内2,4-DHB积累的MG1655生长速率的影响。符号(-)：意指与不存在2,4-DHB的生长速率相比，在2,4-DHB存在下生长减缓5％；(--)：意指生长减缓10％；(＝)：意指相同生长速率(培养基中的DHB浓度无影响)；(+)：意指生长加快5％，并且(++)：意指在2,4-DHB的存在下生长加快10％。

如上表9可见，在10g/L 2,4-DHB的存在下的生长速率在编码特定外排系统的基因过表达时(菌株35至38)恢复，而且当一个摄取系统被缺失并且外排系统过量产生时(菌株120至122)甚至改善了一点。

对于所有外排系统测试减少细菌中的2,4-DHB积累的两种修饰的组合，其具有与上面显示的相似结果(不显示菌株123至131的数据)。

表10：培养基中10g/L的2,4-DHB对经修饰以减少细胞内2,4-DHB积累的的2,4-DHB产生菌株的生长速率的影响。符号(-)：意指与不存在2,4-DHB的生长速率相比，在2,4-DHB存在下生长减缓5％；(--)：意指生长减缓10％；(＝)：意指相同生长速率(培养基中的DHB浓度无影响)；(+)：意指生长加快5％，并且(++)：意指在2,4-DHB的存在下生长加快10％。

如上表10可见，以途径2或3修饰用于产生2,4-DHB的菌株5和6对高浓度2,4-DHB敏感，因为它们的生长速率在10g/L 2,4-DHB的存在下降低。虽然如此，它们的相对生长速率可在编码特定外排系统的基因过表达时恢复，而且当一个摄取系统被缺失并且外排系统过量产生时甚至改善了一点。我们发现减少细胞内部的2,4-DHB积累的修饰改善菌株的生长速率。

对于所有产生菌株(途径1和4)测试减少细菌中2,4-DHB积累的两个修饰的组合，其具有与上面显示的相似结果(不显示菌株8至23和31至34；菌株39至86和菌株108至119的数据)。

表11：对于各培养物，培养基中的2,4-二羟基-丁酸(DHB)浓度。

如上表11可见，对于各2,4-DHB产生菌株，当DHB输出系统过表达时或通过过表达外排系统和缺失摄取系统的组合改善了2,4-二羟基-丁酸(DHB)的产生。

对携带至少一个修饰以减少细胞中的2,4-DHB积累的产生菌株(途径1、2、3和4)的所有不同背景，观察到DHB产生的改善。

根据以下方案对于所有测试的菌株测量细胞内的2,4-DHB浓度。

-在指数期收集样本并储存在冰上(没有淬火)

-在4℃用浓缩3X的生理盐溶液进行两个洗涤步骤以防止我们与细胞一起洗涤的代谢产物渗漏。

进行一些试验以在水、生理水和浓缩3X、5X或10X的生理水之间选择最佳的溶液。谷氨酸盐用作参考(Bolten等,2007)。

-回收细胞沉淀，并保存在-20℃或提取

-在HEPES缓冲液中用热的乙醇来完成提取

-通过GCMS来完成2,4-DHB的定量

在细胞的胞质容积计算中使用的理论值是2.3-2.5mL/g的细胞干重。

菌株的结果与各突变一致。在过表达2,4-DHB外排系统以及过表达输出与弱化2,4-DHB摄取系统的组合存在的情况下，所述化合物的细胞内浓度与未经修饰的微生物相比降低。

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序列表

<110> 代谢探索者公司(METABOLIC EXPLORER)

<120> 用于具有增加的2,4-二羟基丁酸外排物的优化的2,4-二羟基丁酸产生的修饰的微生物

<130> D34827

<140> PCT EP2016/057660

<141> 2016-04-07

<150> EP 15305514.0

<151> 2015-04-07

<160> 250

<170> PatentIn 3.5版

<210> 1

<211> 402

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 1

Met Thr Pro Ser Asn Tyr Gln Arg Thr Arg Trp Leu Thr Leu Ile Gly

1 5 10 15

Thr Ile Ile Thr Gln Phe Ala Leu Gly Ser Val Tyr Thr Trp Ser Leu

20 25 30

Phe Asn Gly Ala Leu Ser Ala Lys Leu Asp Ala Pro Val Ser Gln Val

35 40 45

Ala Phe Ser Phe Gly Leu Leu Ser Leu Gly Leu Ala Ile Ser Ser Ser

50 55 60

Val Ala Gly Lys Leu Gln Glu Arg Phe Gly Val Lys Arg Val Thr Met

65 70 75 80

Ala Ser Gly Ile Leu Leu Gly Leu Gly Phe Phe Leu Thr Ala His Ser

85 90 95

Asp Asn Leu Met Met Leu Trp Leu Ser Ala Gly Val Leu Val Gly Leu

100 105 110

Ala Asp Gly Ala Gly Tyr Leu Leu Thr Leu Ser Asn Cys Val Lys Trp

115 120 125

Phe Pro Glu Arg Lys Gly Leu Ile Ser Ala Phe Ala Ile Gly Ser Tyr

130 135 140

Gly Leu Gly Ser Leu Gly Phe Lys Phe Ile Asp Thr Gln Leu Leu Glu

145 150 155 160

Thr Val Gly Leu Glu Lys Thr Phe Val Ile Trp Gly Ala Ile Ala Leu

165 170 175

Leu Met Ile Val Phe Gly Ala Thr Leu Met Lys Asp Ala Pro Lys Gln

180 185 190

Glu Val Lys Thr Ser Asn Gly Val Val Glu Lys Asp Tyr Thr Leu Ala

195 200 205

Glu Ser Met Arg Lys Pro Gln Tyr Trp Met Leu Ala Val Met Phe Leu

210 215 220

Thr Ala Cys Met Ser Gly Leu Tyr Val Ile Gly Val Ala Lys Asp Ile

225 230 235 240

Ala Gln Ser Leu Ala His Leu Asp Val Val Ser Ala Ala Asn Ala Val

245 250 255

Thr Val Ile Ser Ile Ala Asn Leu Ser Gly Arg Leu Val Leu Gly Ile

260 265 270

Leu Ser Asp Lys Ile Ala Arg Ile Arg Val Ile Thr Ile Gly Gln Val

275 280 285

Ile Ser Leu Val Gly Met Ala Ala Leu Leu Phe Ala Pro Leu Asn Ala

290 295 300

Val Thr Phe Phe Ala Ala Ile Ala Cys Val Ala Phe Asn Phe Gly Gly

305 310 315 320

Thr Ile Thr Val Phe Pro Ser Leu Val Ser Glu Phe Phe Gly Leu Asn

325 330 335

Asn Leu Ala Lys Asn Tyr Gly Val Ile Tyr Leu Gly Phe Gly Ile Gly

340 345 350

Ser Ile Cys Gly Ser Ile Ile Ala Ser Leu Phe Gly Gly Phe Tyr Val

355 360 365

Thr Phe Tyr Val Ile Phe Ala Leu Leu Ile Leu Ser Leu Ala Leu Ser

370 375 380

Thr Thr Ile Arg Gln Pro Glu Gln Lys Met Leu Arg Glu Ala His Gly

385 390 395 400

Ser Leu

<210> 2

<211> 1209

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 2

atgacacctt caaattatca gcgtacccgc tggctgacac tcatcggtac tatcattacc 60

cagtttgcgc tggggtcggt ttatacctgg agcctgttta atggcgcgct ttccgccaag 120

ctggatgcgc cggtaagcca ggtcgctttc tctttcggct tgttaagtct ggggctggca 180

atttcgtctt ctgttgcggg caaattacag gaacgttttg gcgttaaacg cgtcaccatg 240

gcttccggca ttttgctggg attaggcttc ttcctgacag cgcattctga caacctgatg 300

atgctgtggt taagcgccgg tgtgctggtg ggactggcag atggcgcggg ttatctgctg 360

acgctctcta actgtgtgaa gtggttcccg gagcgtaaag gtctgatctc cgcgttcgct 420

atcggttctt atggtctggg tagcctgggt ttcaaattta tcgacacgca gctgctggaa 480

acggtcggtc tggaaaaaac ctttgtgatt tggggagcga ttgcgctgtt gatgattgtt 540

ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag tgaaaaccag caatggtgtg 600

gtggagaaag attacacgct ggcagagtcg atgcgtaaac cgcagtactg gatgttagcg 660

gtaatgttcc tgaccgcctg catgagcggc ctgtacgtga ttggggtagc gaaagatatc 720

gcccaaagtc tggcacacct tgatgtggtt tccgcagcca atgcagtcac tgttatttcc 780

atcgccaacc tttcaggtcg tctggtgctg ggtattctgt ctgacaaaat cgcccgtatc 840

cgtgttatta ccattggtca ggtgatatcg ctggtgggta tggcggccct gctgtttgca 900

ccattgaatg cagtgacgtt ctttgcagcg attgcctgcg tggcatttaa ctttggcggc 960

actattaccg tctttccgtc actggtcagt gagttctttg gcctcaataa cctggcgaaa 1020

aactacggtg tgatttatct cggtttcggt atcggtagca tttgtggttc gattatcgcc 1080

tcactgtttg gcggcttcta tgtgaccttc tacgtgattt tcgccctgct gattctgtca 1140

ttggcgcttt ctacgacgat tcgtcagcca gagcagaaaa tgttgcgtga ggcgcatggc 1200

tccctttaa 1209

<210> 3

<211> 486

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 3

Met Pro Leu Ser Lys Val Glu His Tyr Leu Ser Tyr His Thr Arg Leu

1 5 10 15

Leu Leu Pro His Val Leu Ser Leu Gln Ser Ser His Arg Val Ala Tyr

20 25 30

Ile Phe Ser Leu Leu Ser Ala Val Ser Thr Gly Phe Ile Thr Leu Ile

35 40 45

Ser Leu Tyr Ser Gln Pro Trp Gln Lys His Leu Asn Tyr Ser Ser Trp

50 55 60

Gln Ile Asn Thr Ile Ala Ser Met Thr Asn Leu Gly Met Tyr Leu Thr

65 70 75 80

Pro Pro Ile Leu Gly Met Ile Ala Asp Ser His Gly Pro Ile Thr Leu

85 90 95

Ser Leu Leu Ala Ile Ile Gly Phe Ile Pro Ser Tyr Ser Tyr Leu Ala

100 105 110

Tyr Val Phe Asn His Pro Glu Leu Ser Leu Gly Gly Asn Gly Asp Ser

115 120 125

Ser Phe Asn Leu Ser Ile Ile Cys Phe Val Phe Ile Gly Ile Ser Thr

130 135 140

Ser Ala Leu Tyr Phe Ser Ala Leu Leu Thr Cys Thr Lys Leu Tyr Pro

145 150 155 160

His Thr Lys Leu Leu Ser Ile Ser Leu Pro Thr Thr Cys Tyr Gly Ile

165 170 175

Ser Ser Val Val Gly Ser Gln Leu Leu Arg Ile Lys Trp Phe Trp Ser

180 185 190

Ser Asn Ala Ser Ser Ser Ser Ser Asn Ser Asp Leu Asn Leu Gly Arg

195 200 205

Val Phe Gln Thr Phe Ala Leu Val Tyr Val Val Ile Gly Leu Leu Ala

210 215 220

Trp Ile Ala Thr Ser Val Val Ser Leu Leu His Phe Asn Glu Glu Gln

225 230 235 240

Asp Asn Gln Lys Arg Leu Asp Asp Gln Thr Asp Val Glu Gln Ser Pro

245 250 255

Leu Leu Glu Arg Ser Asn His Val Gln Glu Lys Phe Thr Gln Thr Met

260 265 270

Leu Arg Ile Phe Ser Asp Pro Val Thr Tyr Ile Leu Ala Val Ser Ile

275 280 285

Leu Leu Ser Leu Gly Pro Leu Glu Met Phe Ile Ala Asn Met Gly Ser

290 295 300

Leu Thr Asn Leu Leu Val Gln Leu Asp Ala Pro Thr Leu Ser Thr Lys

305 310 315 320

Leu Leu Ser Thr Tyr Ala Leu Ser Ser Thr Phe Thr Arg Leu Leu Thr

325 330 335

Gly Ile Val Ala Asp Phe Phe Ala Lys Lys Lys Ile Ser Ile Lys Trp

340 345 350

Ile Leu Leu Thr Phe Leu Ser Leu Gly Val Cys Ala Gln Leu Phe Leu

355 360 365

Leu Lys Met Thr Ser Ser Ala Ser Pro Trp Gly Leu Val Pro Thr Gly

370 375 380

Ser Leu Val Gly Ile Val Tyr Gly Gly Leu Phe Thr Val Tyr Pro Thr

385 390 395 400

Leu Val Leu Leu Val Trp Gly Glu Arg Ser Phe Gly Thr Val Tyr Gly

405 410 415

Ser Leu Leu Ile Ala Pro Ala Ile Gly Ser Met Ile Phe Cys Met Leu

420 425 430

Tyr Ala Lys Phe Tyr Asp Ser Arg Cys Met Ser Gly Gly Gly Asp Leu

435 440 445

Arg Asn Pro Ser Cys Ile Ser Ala Val Tyr Lys Tyr Ser Ser Ile Ala

450 455 460

Phe Val Val Ser Ala Val Leu Ser Ala Val Val Phe Trp Lys Leu Lys

465 470 475 480

Ser Arg Lys Leu Arg Ile

485

<210> 4

<211> 1461

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 4

atgcctctat caaaggtgga gcactacctt tcataccata cgcgcttact cttaccccat 60

gttttgtctc ttcagtcatc acatcgtgtt gcatacatct tttcgctatt atctgcggtg 120

tcaactggct tcattacttt gatatctctt tactctcaac cgtggcagaa acatttaaat 180

tattcctcat ggcaaatcaa caccatcgct agtatgacta atttggggat gtacttgacg 240

ccaccaatct tggggatgat cgctgattct catggcccca ttactttaag tcttttagcc 300

atcatagggt tcatacctag ctattcatat ctggcttacg tttttaatca tccggagtta 360

tctctcggag gaaatggtga ctcatcattc aatctatcca tcatttgttt cgttttcata 420

ggtatatcaa caagcgcttt atactttagc gctttactga catgcactaa gctatatcct 480

catacaaaac tactatccat tagcttacca acgacatgtt atggtatttc ttctgtagtc 540

ggttctcaac tgctaagaat caaatggttc tggtcctcta acgcaagttc ttcctcgtcc 600

aatagtgact taaacctggg aagagtattc caaacatttg ccctcgttta tgtcgttatt 660

gggctacttg catggatagc caccagcgtg gtatcacttt tgcattttaa tgaagagcaa 720

gacaaccaaa aacggctgga tgatcaaact gatgtggaac aatcaccgct gttagaacga 780

agtaatcatg ttcaagaaaa gtttacgcag acgatgctaa ggatctttag tgatcctgtg 840

acatatatcc tagcggtatc aattttgtta tcacttgggc ccctcgagat gtttattgcc 900

aatatgggat cactgactaa cctgctagtc caattagatg cgccaacctt atctacaaag 960

ttgttatcca catacgcgct atcttccact tttacgagat tgctcacagg catagtggca 1020

gacttcttcg ccaagaaaaa aatatcaatt aaatggatcc tgttgacttt cctttcatta 1080

ggggtatgtg cacaactgtt tttattgaaa atgacctctt cagcgtcacc ctgggggcta 1140

gtacctacag gatcattggt tggaattgta tacggtggac ttttcactgt ttatccgacg 1200

ctggtcctgt tagtatgggg cgaacgctca ttcgggactg tttacggtag cttactaatt 1260

gcacctgcta taggttctat gatattttgc atgttgtatg ccaaatttta cgattctcgc 1320

tgtatgagtg gcggaggaga tctgcgaaat ccgtcctgta tttcggctgt ctacaagtac 1380

agcagtatcg cattcgttgt atccgctgtt ctttcagctg tagtattttg gaaattaaaa 1440

agtagaaaac tcagaattta a 1461

<210> 5

<211> 473

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 5

Met Ser Glu Glu Arg His Glu Asp His His Arg Asp Val Glu Asn Lys

1 5 10 15

Leu Asn Leu Asn Gly Lys Asp Asp Ile Asn Gly Asn Thr Ser Ile Ser

20 25 30

Ile Glu Val Pro Asp Gly Gly Tyr Gly Trp Phe Ile Leu Leu Ala Phe

35 40 45

Ile Leu Tyr Asn Phe Ser Thr Trp Gly Ala Asn Ser Gly Tyr Ala Ile

50 55 60

Tyr Leu Ala His Tyr Leu Glu Asn Asn Thr Phe Ala Gly Gly Ser Lys

65 70 75 80

Leu Asp Tyr Ala Ser Ile Gly Gly Leu Ala Phe Ser Cys Gly Leu Phe

85 90 95

Phe Ala Pro Val Ile Thr Trp Leu Tyr His Ile Phe Ser Ile Gln Phe

100 105 110

Ile Ile Gly Leu Gly Ile Leu Phe Gln Gly Ala Ala Leu Leu Leu Ala

115 120 125

Ala Phe Ser Val Thr Leu Trp Glu Ile Tyr Leu Thr Gln Gly Val Leu

130 135 140

Ile Gly Phe Gly Leu Ala Phe Ile Phe Ile Pro Ser Val Thr Leu Ile

145 150 155 160

Pro Leu Trp Phe Arg Asn Lys Arg Ser Leu Ala Ser Gly Ile Gly Thr

165 170 175

Ala Gly Ser Gly Leu Gly Gly Ile Val Phe Asn Leu Gly Met Gln Ser

180 185 190

Ile Leu Gln Lys Arg Gly Val Lys Trp Ala Leu Ile Ala Gln Cys Ile

195 200 205

Ile Cys Thr Ser Leu Ser Thr Ile Ala Leu Met Leu Thr Arg Thr Thr

210 215 220

His Gln Gly Leu Arg Gln His Lys Arg Ser Tyr Lys Phe Glu Leu Leu

225 230 235 240

Asp Tyr Asp Val Leu Ser Asn Phe Ala Val Trp Leu Leu Phe Gly Phe

245 250 255

Val Ser Phe Ala Met Leu Gly Tyr Val Val Leu Leu Tyr Ser Leu Ser

260 265 270

Asp Phe Thr Val Ser Leu Gly Tyr Thr Ser Lys Gln Gly Ser Tyr Val

275 280 285

Ser Cys Met Val Ser Val Gly Ser Leu Leu Gly Arg Pro Ile Val Gly

290 295 300

His Ile Ala Asp Lys Tyr Gly Ser Leu Thr Val Gly Met Ile Leu His

305 310 315 320

Leu Val Met Ala Ile Leu Cys Trp Ala Met Trp Ile Pro Cys Lys Asn

325 330 335

Leu Ala Thr Ala Ile Ala Phe Gly Leu Leu Val Gly Ser Ile Met Gly

340 345 350

Thr Ile Trp Pro Thr Ile Ala Ser Ile Val Thr Arg Ile Val Gly Leu

355 360 365

Gln Lys Leu Pro Gly Thr Phe Gly Ser Thr Trp Ile Phe Met Ala Ala

370 375 380

Phe Ala Leu Val Ala Pro Ile Ile Gly Leu Glu Leu Arg Ser Thr Asp

385 390 395 400

Thr Asn Gly Asn Asp Tyr Tyr Arg Thr Ala Ile Phe Val Gly Phe Ala

405 410 415

Tyr Phe Gly Val Ser Leu Cys Gln Trp Leu Leu Arg Gly Phe Ile Ile

420 425 430

Ala Arg Asp Glu Ile Ala Val Arg Glu Ala Tyr Ser Ala Asp Gln Asn

435 440 445

Glu Leu His Leu Asn Val Lys Leu Ser His Met Ser Lys Cys Leu Phe

450 455 460

Arg Tyr Lys Gln Leu Pro Arg Arg Val

465 470

<210> 6

<211> 1422

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 6

atgtccgaag aacggcatga agatcatcat agggatgttg aaaataaatt gaatttaaat 60

ggtaaagacg atattaacgg gaatacctca atctcgatcg aggtgcctga tggaggatat 120

gggtggttta ttcttcttgc ttttattctg tacaactttt ctacttgggg ggcaaattct 180

ggttatgcta tttatttagc gcattattta gagaataata cttttgctgg tgggagtaaa 240

ttagactatg cttctatagg tgggttagca ttcagttgtg gacttttttt tgccccagtt 300

ataacatggc tttatcatat attttcaatt caattcatta taggcttagg gatactgttt 360

caaggggcag cgctactgct tgcagctttt tctgtcacac tctgggaaat ttatctcacg 420

caaggcgttt taattggatt cggtttagca tttattttca tacccagtgt cacactcatc 480

ccactatggt tcagaaataa aagatcttta gcctctggta taggaactgc tggaagcggg 540

ttaggtggta ttgtctttaa cttgggaatg caaagtattc tacaaaagag gggcgttaaa 600

tgggcgctca ttgctcagtg cataatatgc acatcactta gcaccattgc gcttatgttg 660

accagaacaa cacatcaagg cctacgtcaa cataagagat cttacaaatt tgaattgcta 720

gattatgatg tgctttcaaa tttcgcggtc tggttacttt ttggatttgt atcatttgct 780

atgttaggat atgttgtcct tttgtattcc ttgtctgatt ttaccgttag tttaggttat 840

actagtaagc aaggctcata cgtatcgtgc atggtgagtg tcggctctct gctgggacga 900

ccaattgtgg gtcacattgc tgataaatat ggatcactaa cagttggcat gatattgcac 960

cttgtcatgg ccatcctttg ttgggccatg tggatacctt gtaaaaattt ggccactgcg 1020

atagcttttg gattattggt tggttctatt atgggaacaa tttggccaac aattgcttca 1080

attgttacac gcattgttgg tcttcaaaag cttcctggta cctttggtag tacctggatt 1140

tttatggcgg cttttgcctt agttgccccc ataatcggtc tggaacttcg ttcaactgat 1200

acgaatggaa acgattatta tcgtacagca atattcgtgg gttttgcgta ctttggtgtt 1260

agtttatgcc aatggctatt gagagggttt ataatagctc gagatgagat tgctgtgcgt 1320

gaagcctatt cagctgacca aaatgaattg catttaaacg ttaagttatc acatatgagt 1380

aaatgtcttt ttcgttataa acaattacct aggagagtct aa 1422

<210> 7

<211> 673

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 7

Met Ser Thr His Ser Asn Asp Tyr Phe Ser Ala Ser Ser Gly Met Val

1 5 10 15

Ser Glu Thr Ser Ser Glu Val Ser Ser Ile Asn Ser Ser Gln Pro Val

20 25 30

Ser Phe Ser Lys Ala Ser Ile Ala Ala Pro Val Pro Cys Ser Asp Leu

35 40 45

His Ser Thr Lys Ser Asn Asp Ala Ser Arg Lys Leu Ser Ile Ser Arg

50 55 60

Thr Leu Thr Asn Arg Leu Asn Asp Ile Lys Lys Ala Val Asp Asp Asp

65 70 75 80

Asn Leu Gln Thr Glu Glu Asn Ser Ala Asp Val Asn Lys Ile Leu Glu

85 90 95

Ser Arg Phe Asp Val Ala Asp Ala Ile Arg Leu Gln His Asn Glu Ser

100 105 110

Val Gln Ser Lys Leu Asn Ile Pro Val Thr His Thr Thr Thr Ala Gly

115 120 125

Ala Ser Leu Ser Ala Pro Ser Ser Ser Ala Phe Ser Ala Ser Ser Ile

130 135 140

Gln Asn Asp Thr Thr Glu His Lys Ala Ser Met Asp Ser Lys Leu Met

145 150 155 160

Arg Asn Arg Leu Tyr Pro Ala Ser Thr Lys His Ser Gly Lys Asp Leu

165 170 175

Glu Ala Gln Gly Ile Thr Glu Phe Glu Pro Asp Glu Pro Thr Val Lys

180 185 190

Lys Val Phe Thr Asn Lys Ser Thr Gly Gln Leu Glu Leu Pro Pro Asp

195 200 205

Gly Gly Tyr Gly Trp Val Val Thr Phe Cys Val Phe Leu Thr Met Phe

210 215 220

Ser Thr Trp Gly Cys Asn Ala Ser Phe Gly Val Asp Leu Ala Tyr Tyr

225 230 235 240

Leu Asn His Asp Thr Tyr Pro Gly Ala Ser Lys Tyr Asp Tyr Ala Leu

245 250 255

Ile Ala Gly Leu Thr Val Phe Leu Gly Gln Leu Leu Ser Pro Leu Val

260 265 270

Met Ala Leu Met Arg Ile Ile Gly Leu Arg Thr Thr Met Leu Phe Gly

275 280 285

Asp Ala Val Met Leu Ala Ala Tyr Leu Leu Ala Ser Phe Thr Thr Lys

290 295 300

Leu Trp Gln Leu Tyr Val Thr Gln Gly Phe Met Val Gly Cys Ser Ile

305 310 315 320

Ser Leu Ile Phe Val Pro Ala Thr Thr Val Leu Pro Gly Trp Phe Leu

325 330 335

Lys Lys Arg Ala Val Ala Met Gly Val Ser Leu Leu Gly Thr Gly Ala

340 345 350

Gly Gly Val Val Tyr Gly Leu Ala Thr Asn Lys Met Leu Ser Asp Phe

355 360 365

Gly Asn Thr Arg Trp Cys Leu Arg Ile Ile Gly Ile Ser Cys Ser Ile

370 375 380

Ser Val Leu Val Ala Ile Ala Leu Leu Lys Glu Arg Asn Pro Thr Pro

385 390 395 400

Ala Ile Gly Leu Lys Ser Pro Arg Ala Met Phe Glu Gln Leu Lys Ala

405 410 415

Met Phe Ser Leu Lys Val Ile Thr Lys Pro Phe Val Val Leu Ile Ala

420 425 430

Leu Trp Phe Met Phe Ala Leu Phe Ala Tyr Asn Met Met Val Phe Thr

435 440 445

Leu Ser Ser Tyr Ala Ile Ser Lys Gly Leu Ser Ser His Asp Ala Ser

450 455 460

Thr Leu Thr Ala Ile Leu Asn Gly Ser Gln Ser Ile Gly Arg Pro Leu

465 470 475 480

Met Gly Leu Ala Gly Asp Lys Phe Gly Arg Ala Asn Val Thr Ile Val

485 490 495

Leu Thr Thr Leu Leu Thr Ile Tyr Met Phe Ala Phe Trp Ile Pro Ala

500 505 510

His Thr Phe Val Gln Leu Ile Phe Phe Ser Ile Leu Val Gly Ser Cys

515 520 525

Val Gly Val Ala Asn Val Met Asn Thr Val Leu Ile Ala Asp Met Val

530 535 540

Lys Pro Glu Glu Phe Leu Pro Ala Trp Ala Phe Val Asn Tyr Cys Gly

545 550 555 560

Ala Pro Phe Leu Leu Val Cys Glu Val Ile Ala Gln Ala Leu Thr Val

565 570 575

Glu Lys Asp Lys Ser Asn Pro Tyr Leu His Ala Gln Ile Phe Cys Gly

580 585 590

Cys Cys Phe Ile Ala Ala Leu Ile Leu Ile Ser Ile Leu Arg Glu Tyr

595 600 605

Ser Ile Arg Met Lys Leu Thr Glu Arg Gln Ala Met Thr Asn Glu Lys

610 615 620

Leu Lys Glu Trp Lys Ala Ser Glu Tyr Asp Thr Asp Ser Ala Asp Glu

625 630 635 640

Asp Trp Gly Lys Leu Lys Glu Arg Lys Thr Lys Tyr Asp Leu Leu Leu

645 650 655

Gly Pro Gly Ile Lys Lys Tyr Phe Leu Arg Met Val Tyr Pro Met Lys

660 665 670

Val

<210> 8

<211> 2022

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 8

atgtcaacgc actcaaacga ctacttttct gcttcttccg gaatggtctc tgagacatcg 60

tccgaggttt cttcgataaa ctcttcacag cctgtatcat tctctaaggc ttctattgct 120

gctccggttc catgctctga tctacacagc accaagtcga acgatgcatc gagaaaattg 180

tctattagta ggacgttaac taatcggctc aacgacatta aaaaggctgt cgatgacgac 240

aacttgcaga cggaagaaaa ttccgcagac gttaataaaa tattagaatc tagatttgac 300

gtggccgatg ccattaggct acagcacaat gagtcagtcc agtcaaagtt aaacatccca 360

gtcacacaca ccacgactgc aggcgcctcg ttgtcggcac catcttcctc tgctttctct 420

gcttcttcta ttcaaaatga tactacagaa cataaagctt ccatggactc caaactcatg 480

aggaatagac tatatccggc ttccacgaaa cactccggta aggatcttga ggcccaagga 540

ataaccgaat tcgagcctga tgaaccgact gtaaaaaaag tattcaccaa caagtctacc 600

gggcagctgg aactgccccc cgacggtggt tatggctggg tcgtgacatt ctgtgtgttc 660

ttgaccatgt tttccacgtg gggctgcaac gcatccttcg gtgtcgacct tgcctactac 720

ttaaaccatg atacttaccc tggtgcttcg aagtacgatt atgccttaat tgctggccta 780

actgtctttc tcggtcaact cttatccccc cttgtgatgg cactgatgag aataattggt 840

ctgcggacca ccatgctttt tggtgatgct gtaatgcttg ccgcatatct cttggcctcc 900

tttactacca agttatggca attgtatgtc acccaaggtt ttatggtcgg ttgttcaata 960

tcactgattt tcgttccagc aacaaccgtc ttaccaggat ggttcttgaa aaaaagagct 1020

gtcgcaatgg gtgtctcatt attgggtacc ggtgctggtg gtgtcgttta cggtttggct 1080

acaaacaaaa tgctttctga ctttggaaat acccggtggt gccttcgtat cataggcata 1140

tcgtgtagca taagtgttct agttgctatt gcgctcttaa aagaaagaaa ccctacacct 1200

gccataggat tgaaatcgcc tcgggccatg tttgaacagc tcaaagcaat gttttcatta 1260

aaggttataa ctaagccatt tgtggtactt attgcattat ggttcatgtt cgcattattt 1320

gcctacaata tgatggtttt tactttatct tcatacgcaa tctcgaaagg attatcatcg 1380

cacgacgctt ccacattgac tgccattttg aacggctcgc aatccatcgg aagacctctg 1440

atgggtttag cgggagataa gtttggtagg gcaaacgtaa cgatcgtatt aaccactttg 1500

ttaacaatat atatgtttgc gttctggatc cccgctcata cgtttgttca actcatcttt 1560

ttttcaattc tagttggctc atgcgttggt gtcgccaacg tcatgaatac cgttctgatt 1620

gccgatatgg ttaaaccaga agagtttttg cccgcttggg ccttcgtcaa ctactgtggt 1680

gcgcctttct tattggtttg tgaggtgatt gcccaggcat tgacggtgga gaaagataag 1740

agcaatcctt acttacatgc acaaattttt tgcggttgct gctttattgc cgcactaatt 1800

ttaatttcta tccttcgtga atattctatc aggatgaaat taacggaaag acaagctatg 1860

acaaacgaga agttaaaaga atggaaggca agcgaatacg ataccgattc tgccgatgaa 1920

gattggggta aattaaaaga aagaaagact aaatatgacc ttcttttagg tccgggcatt 1980

aaaaaatact tcctaagaat ggtatatcca atgaaggtct ag 2022

<210> 9

<211> 521

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 9

Met Ser Ser Asp Ser Leu Thr Pro Lys Asp Thr Ile Val Pro Glu Glu

1 5 10 15

Gln Thr Asn Gln Leu Arg Gln Pro Asp Leu Asp Glu Asp Ser Ile His

20 25 30

Tyr Asp Pro Glu Ala Asp Asp Leu Glu Ser Leu Glu Thr Thr Ala Ser

35 40 45

Tyr Ala Ser Thr Ser Val Ser Ala Lys Val Tyr Thr Lys Lys Glu Val

50 55 60

Asn Lys Gly Thr Asp Ile Glu Ser Gln Pro His Trp Gly Glu Asn Thr

65 70 75 80

Ser Ser Thr His Asp Ser Asp Lys Glu Glu Asp Ser Asn Glu Glu Ile

85 90 95

Glu Ser Phe Pro Glu Gly Gly Phe Lys Ala Trp Val Val Thr Phe Gly

100 105 110

Cys Phe Leu Gly Leu Ile Ala Cys Phe Gly Leu Leu Asn Ser Thr Gly

115 120 125

Val Ile Glu Ser His Leu Gln Asp Asn Gln Leu Ser Ser Glu Ser Val

130 135 140

Ser Thr Ile Gly Trp Leu Phe Ser Leu Phe Leu Phe Val Cys Ser Ala

145 150 155 160

Ser Cys Ile Ile Ser Gly Thr Tyr Phe Asp Arg Asn Gly Phe Arg Thr

165 170 175

Ile Met Ile Val Gly Thr Val Phe His Val Ala Gly Leu Phe Ala Thr

180 185 190

Ala Asn Ser Thr Lys Tyr Trp His Phe Ile Leu Ser Phe Ala Ile Val

195 200 205

Cys Gly Phe Gly Asn Gly Ile Val Leu Ser Pro Leu Val Ser Val Pro

210 215 220

Ala His Tyr Phe Phe Lys Arg Arg Gly Thr Ala Leu Ala Met Ala Thr

225 230 235 240

Ile Gly Gly Ser Val Gly Gly Val Val Phe Pro Ile Met Leu Arg Ser

245 250 255

Phe Phe Ser Met Lys Ser Asp Thr Asp Pro Thr Tyr Gly Phe Val Trp

260 265 270

Gly Ile Arg Thr Leu Gly Phe Leu Asp Leu Ala Leu Leu Thr Leu Ser

275 280 285

Ile Ile Leu Val Lys Glu Arg Leu Pro His Val Ile Glu Asn Ser Lys

290 295 300

Asp Gly Glu Ser Arg Trp Arg Tyr Ile Leu Arg Val Tyr Ile Leu Gln

305 310 315 320

Cys Phe Asp Ala Lys Ala Phe Leu Asp Met Lys Tyr Leu Phe Cys Val

325 330 335

Leu Gly Thr Val Phe Ser Glu Leu Ser Ile Asn Ser Ala Leu Thr Tyr

340 345 350

Tyr Gly Ser Tyr Ala Thr Ser His Gly Ile Ser Ala Asn Asp Ala Tyr

355 360 365

Thr Leu Ile Met Ile Ile Asn Val Cys Gly Ile Pro Gly Arg Trp Val

370 375 380

Pro Gly Tyr Leu Ser Asp Lys Phe Gly Arg Phe Asn Val Ala Ile Ala

385 390 395 400

Thr Leu Leu Thr Leu Phe Ile Val Met Phe Val Gly Trp Leu Pro Phe

405 410 415

Gly Thr Asn Leu Thr Asn Met Tyr Val Ile Ser Ala Leu Tyr Gly Phe

420 425 430

Cys Ser Gly Ser Val Phe Ser Leu Leu Pro Val Cys Cys Gly Gln Ile

435 440 445

Ser Lys Thr Glu Glu Phe Gly Lys Arg Tyr Ser Thr Met Tyr Phe Val

450 455 460

Val Gly Phe Gly Thr Leu Val Gly Ile Pro Ile Thr Gly Ala Ile Ile

465 470 475 480

Ser Ile Lys Thr Thr Ala Asp Tyr Gln His Tyr Ile Ile Phe Cys Gly

485 490 495

Leu Ala Thr Phe Val Ser Ala Val Cys Tyr Ile Ile Ser Arg Ala Tyr

500 505 510

Cys Val Gly Phe Lys Trp Val Arg Phe

515 520

<210> 10

<211> 1566

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 10

atgagctcag acagtttaac gcctaaagac actatagttc cagaagaaca aaccaaccaa 60

ctgcggcaac ctgatttaga tgaggatagc atacattatg atccagaagc tgatgattta 120

gagtcattag agactactgc ctcttacgcg tcaacgtcgg tgtcggcaaa ggtgtatacc 180

aagaaggaag taaataaagg aactgacatt gaaagccaac cacattgggg tgaaaatacc 240

tcgagcacgc acgacagtga caaagaagaa gattccaatg aagaaattga aagcttcccg 300

gagggtggat tcaaggcttg ggttgtgaca tttggttgct tcttgggtct aattgcatgc 360

tttggtttat taaactcaac gggggtcata gaaagtcatc tacaggacaa tcaattatcc 420

agtgaaagtg tttctaccat tggttggctg ttttctttgt ttctttttgt ttgctctgcc 480

tcatgtatca ttagtggtac ttactttgat agaaatggat ttaggacaat tatgattgtg 540

ggcacggttt ttcatgtagc cggtctgttc gctaccgcca actctactaa atattggcac 600

ttcatactgt cctttgccat tgtctgcggg ttcggtaatg gtattgtact gagtccccta 660

gtaagtgttc ccgcccacta tttttttaag agacgtggta ctgcattggc aatggccact 720

attggtggta gtgtgggtgg cgtcgttttc ccaattatgt tgcgtagttt cttctctatg 780

aagtcagaca ctgatccaac ttatggattt gtttggggca ttagaacttt aggattttta 840

gatttggctt tgctaactct gtcaatcatt ttagtcaaag agaggttacc acatgttatc 900

gaaaattcca aagatggtga gtcacgttgg agatacatac tcagagttta tattctgcaa 960

tgttttgatg cgaaggcttt cctggatatg aaatatcttt tttgtgtcct gggaacggta 1020

tttagtgagt tatccattaa ttcagctctt acttattatg gatcatacgc caccagccat 1080

ggaatttctg ctaatgacgc ctacaccttg attatgatta taaatgtctg cggcataccc 1140

gggagatggg ttcctggcta tttgagcgat aagttcggta ggtttaacgt cgcaattgca 1200

actctactca ctctatttat cgtcatgttt gttggttggt taccatttgg taccaatttg 1260

acaaatatgt acgttattag tgccctatat ggattttgtt ctggaagcgt tttctcctta 1320

cttcctgttt gctgtggcca gatatctaaa acggaagagt tcggtaaacg ttactctaca 1380

atgtacttcg ttgtcggttt cggtacttta gtcggcattc cgataacagg tgccattatc 1440

tctatcaaga caacggccga ttaccaacac tatattattt tttgcggttt ggcaactttt 1500

gtaagcgctg tttgctacat aatttcgaga gcatactgtg ttggcttcaa gtgggtcaga 1560

ttttaa 1566

<210> 11

<211> 373

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 11

Met Thr Gln Thr Asp Asn Pro Val Pro Asn Cys Gly Leu Leu Pro Glu

1 5 10 15

Gln Gln Tyr Cys Ser Ala Asp His Glu Glu Pro Leu Leu Leu His Glu

20 25 30

Glu Gln Leu Ile Phe Pro Asp His Ser Ser Gln Leu Ser Ser Ala Asp

35 40 45

Ile Ile Glu Pro Ile Lys Met Asn Ser Ser Thr Glu Ser Ile Ile Gly

50 55 60

Thr Thr Leu Arg Lys Lys Trp Val Pro Leu Ser Ser Thr Gln Ile Thr

65 70 75 80

Ala Leu Ser Gly Ala Phe Ala Gly Phe Leu Ser Gly Val Ala Val Cys

85 90 95

Pro Leu Asp Val Ala Lys Thr Arg Leu Gln Ala Gln Gly Leu Gln Thr

100 105 110

Arg Phe Glu Asn Pro Tyr Tyr Arg Gly Ile Met Gly Thr Leu Ser Thr

115 120 125

Ile Val Arg Asp Glu Gly Pro Arg Gly Leu Tyr Lys Gly Leu Val Pro

130 135 140

Ile Val Leu Gly Tyr Phe Pro Thr Trp Met Ile Tyr Phe Ser Val Tyr

145 150 155 160

Glu Phe Ser Lys Lys Phe Phe His Gly Ile Phe Pro Gln Phe Asp Phe

165 170 175

Val Ala Gln Ser Cys Ala Ala Ile Thr Ala Gly Ala Ala Ser Thr Thr

180 185 190

Leu Thr Asn Pro Ile Trp Val Val Lys Thr Arg Leu Met Leu Gln Ser

195 200 205

Asn Leu Gly Glu His Pro Thr His Tyr Lys Gly Thr Phe Asp Ala Phe

210 215 220

Arg Lys Leu Phe Tyr Gln Glu Gly Phe Lys Ala Leu Tyr Ala Gly Leu

225 230 235 240

Val Pro Ser Leu Leu Gly Leu Phe His Val Ala Ile His Phe Pro Ile

245 250 255

Tyr Glu Asp Leu Lys Val Arg Phe His Cys Tyr Ser Arg Glu Asn Asn

260 265 270

Thr Asn Ser Ile Asn Leu Gln Arg Leu Ile Met Ala Ser Ser Val Ser

275 280 285

Lys Met Ile Ala Ser Ala Val Thr Tyr Pro His Glu Ile Leu Arg Thr

290 295 300

Arg Met Gln Leu Lys Ser Asp Ile Pro Asp Ser Ile Gln Arg Arg Leu

305 310 315 320

Phe Pro Leu Ile Lys Ala Thr Tyr Ala Gln Glu Gly Leu Lys Gly Phe

325 330 335

Tyr Ser Gly Phe Thr Thr Asn Leu Val Arg Thr Ile Pro Ala Ser Ala

340 345 350

Ile Thr Leu Val Ser Phe Glu Tyr Phe Arg Asn Arg Leu Glu Asn Ile

355 360 365

Ser Thr Met Val Ile

370

<210> 12

<211> 1122

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 12

atgacacaga ctgataatcc tgtccccaac tgcggtttac tgcccgagca gcagtattgc 60

tctgcagacc atgaagagcc actgttgttg catgaagaac aattgatatt ccctgatcat 120

tcctcccaac tgtcctcagc agatatcatc gagcccatca agatgaacag cagtactgag 180

tcaattatag ggacaacgct gcgaaagaaa tgggtaccac tatcctcaac tcagatcaca 240

gctctttccg gcgcatttgc tggattctta tcaggtgtgg cagtatgtcc cctcgatgtt 300

gccaaaacgc gattgcaagc acaaggacta caaactaggt tcgagaaccc ctactatagg 360

gggataatgg ggacattaag tactatagta agagacgaag gcccgcgggg cctctacaaa 420

gggctggtac cgattgtcct gggctacttc ccaacctgga tgatatactt ctccgtgtat 480

gaattcagca aaaagttctt tcacggcatc ttcccacagt ttgattttgt tgctcagtca 540

tgtgctgcaa tcacggcagg cgctgcatct accaccttga ccaacccaat ctgggttgtg 600

aagacaagac ttatgctgca atcaaacctc ggtgagcacc ccacacatta caaaggcact 660

ttcgatgcat tcagaaagct attttatcag gaagggttta aagcattata tgcggggctg 720

gtcccctcat tattagggct atttcatgtg gctatccatt tccctatata cgaagatttg 780

aaggtaagat ttcactgcta ttctcgggag aacaacacca actccatcaa cttgcaacgg 840

ttgatcatgg catcgtccgt ctctaagatg attgcatcag cagtaacata tccgcacgaa 900

attttacgaa ccagaatgca actgaaatca gatataccag attccattca acgacgtctg 960

ttccccctca ttaaagcaac ttatgcacaa gagggactaa agggatttta ttctggattt 1020

actactaacc tagtacgaac cattccggcc tcggcaatca ctctagtgtc ctttgagtat 1080

ttcagaaacc gcctagaaaa tattagcact atggtaattt aa 1122

<210> 13

<211> 392

<212> PRT

<213> 黑曲霉(Aspergillus niger)

<400> 13

Met Phe Ser Glu Gly His Gly Pro Ser Gly Ala Ala Ser Gly Ser Gln

1 5 10 15

Glu Gln Gln Gly Thr Thr Ser Asp Ser Ser Pro Ser Ser Thr Leu His

20 25 30

Val Ser Ala Glu Ser Pro Ser Gln Pro Thr Leu Leu Asp Arg Phe Glu

35 40 45

Met Leu Ala Thr Arg Val Pro Asp Tyr Tyr Ile Thr Pro Phe Cys Gly

50 55 60

Ala Ser Ala Gly Val Ala Ser Gly Ile Val Thr Cys Pro Leu Asp Val

65 70 75 80

Ile Lys Thr Lys Leu Gln Ala Gln Gly Gly Phe Ala Arg Arg Arg Gly

85 90 95

Lys Ala Val Glu Ala Lys Thr Leu Tyr Arg Gly Met Leu Gly Thr Gly

100 105 110

Arg Val Ile Trp Arg Glu Asp Gly Ile Arg Gly Leu Tyr Gln Gly Leu

115 120 125

Gly Pro Met Leu Leu Gly Tyr Leu Pro Thr Trp Ala Val Tyr Leu Ala

130 135 140

Val Tyr Asp Arg Ser Arg Glu Tyr Phe Tyr Glu Thr Thr Asp Ser Trp

145 150 155 160

Trp Leu Ser Arg Gly Tyr Ala Ser Ile Thr Ala Gly Ala Cys Ser Thr

165 170 175

Leu Ala Thr Asn Pro Ile Trp Val Ile Lys Thr Arg Leu Met Ser Gln

180 185 190

Ser Leu Arg Ser Ser Ser Glu Gly Tyr Arg Ala Pro Trp Gln Tyr Lys

195 200 205

Asn Thr Trp Asp Ala Ala Arg Lys Met Tyr Arg Ser Glu Gly Ile Arg

210 215 220

Ser Phe Tyr Ser Gly Leu Thr Pro Ala Leu Leu Gly Leu Ala His Val

225 230 235 240

Ala Ile Gln Phe Pro Leu Tyr Glu Tyr Leu Lys Met Ala Phe Thr Gly

245 250 255

Tyr Ser Ile Gly Glu His Pro Asp Thr Gly Ser Ser His Trp Val Gly

260 265 270

Ile Thr Ser Ala Thr Phe Leu Ser Lys Arg Thr Ser Pro Ala Ala Ser

275 280 285

Pro Glu Glu Ile Ser Phe Arg Gly Gly Met Asp His Pro Gln Gly His

290 295 300

Ser Arg Pro Pro Gly Ala Ala Ser Ser Asp Gly Met Pro Asn Arg Pro

305 310 315 320

Arg Tyr Thr Gly Ile Ile Arg Thr Cys Gln Thr Ile Leu Arg Glu Glu

325 330 335

Gly Trp Arg Ala Phe Tyr Ser Gly Ile Gly Thr Asn Leu Phe Arg Ala

340 345 350

Val Pro Ala Ala Met Thr Thr Met Leu Thr Tyr Glu Tyr Leu Lys Lys

355 360 365

Thr Ile Gly His Val Gln His Glu Gly Glu Leu Lys Leu Gln Lys Leu

370 375 380

Glu Ala Thr Ser Asp Ser Gly Ile

385 390

<210> 14

<211> 1179

<212> DNA

<213> 黑曲霉(Aspergillus niger)

<400> 14

atgttctctg aaggccatgg cccttcgggg gctgccagcg gctctcaaga gcagcagggt 60

acaacttcgg acagttcgcc ttcgtcgacg cttcatgtat cggccgagtc cccatcccag 120

cccactcttc tcgatcgctt cgaaatgctc gctacacgtg tgccggatta ttacatcacg 180

ccgttctgcg gggccagtgc gggcgtagcc tcgggaattg tcacctgccc tctcgatgtg 240

atcaagacca agctgcaggc tcagggtggg ttcgcgcgac gacgtggcaa ggcggtcgag 300

gccaaaacac tgtaccgggg catgctagga actggacgag taatatggcg ggaggatggt 360

attcgaggcc tttatcaagg attgggtccc atgctcctgg gatacctgcc gacgtgggcc 420

gtctatttgg cggtctacga tcggtctcgg gagtatttct acgaaacaac agatagttgg 480

tggctctcac gaggttacgc ctccataacg gcgggcgctt gctcgaccct tgcgacgaat 540

ccaatttggg tgattaagac acgacttatg tcacagagcc tccggtcgag cagcgaaggc 600

taccgggctc cttggcaata caaaaacaca tgggatgcgg cccgcaaaat gtacagaagt 660

gaagggattc gttcgttcta ctccggcctt accccagcgc tgctggggct ggcgcatgtg 720

gccatccagt tccctcttta cgaatatctg aagatggcgt ttactggcta tagtattggc 780

gaacatcccg atactggcag ctcacactgg gtgggcatta catctgcgac attcctgagt 840

aagaggacat caccggcggc ctcacccgaa gagatctcgt tccgcggcgg gatggatcac 900

ccccagggtc atagcaggcc cccgggtgct gcttcgtcgg acggaatgcc caatcgaccc 960

cggtacacgg gaatcattcg cacatgccag accattctga gagaagaagg ctggcgcgcg 1020

ttctactctg ggatcggcac gaatctgttc cgggcggtcc cggctgcgat gactaccatg 1080

ctcacttatg agtacttgaa gaaaacgatt ggacacgtgc aacatgaggg agagctgaag 1140

ttgcagaagc tggaggccac atcagacagt ggaatctaa 1179

<210> 15

<211> 316

<212> PRT

<213> 白色念珠菌(Candida albicans)

<400> 15

Met Glu Leu Asn Thr Thr Asp Lys Gln Val His Thr Arg Arg Phe Ser

1 5 10 15

Ser Arg Glu Ile Glu Val Ile Ser Gly Leu Leu Ala Gly Phe Ser Thr

20 25 30

Thr Ile Val Thr His Pro Leu Asp Val Ile Lys Ile Arg Leu Gln Leu

35 40 45

Ser Arg Asp Thr Pro Lys Thr Thr His Pro Leu Glu Ser Ile Ile Ser

50 55 60

Val Ile Lys Lys Ile Asn Gln Asp Ala Lys Val Ala Tyr Lys Leu Asn

65 70 75 80

His Lys Pro Lys Ala Phe Asn Tyr Leu Ile Gln Tyr Tyr Arg Gly Ile

85 90 95

Thr Pro Asn Leu Ile Gly Asn Ile Ser Ala Trp Gly Ile Tyr Phe Ala

100 105 110

Leu Tyr Ala Glu Phe Lys Ser Lys Val Lys Thr Asn Asn Thr Thr Met

115 120 125

Asn Tyr Phe Ala Ser Ser Val Leu Ala Gly Leu Ser Thr Ser Ile Ile

130 135 140

Thr Asn Pro Leu Trp Val Leu Lys Thr Arg Ile Leu Gly Ser Ser Arg

145 150 155 160

Asn Glu Ser Asn Ala Tyr Arg Ser Val Thr Asp Gly Ile Arg Gln Met

165 170 175

Leu Ala Lys Glu Gly Ile Thr Ser Phe Trp Lys Gly Thr Ile Pro Ser

180 185 190

Leu Phe Ser Val Val Gln Ala Ser Leu Gln Ile Thr Ile Tyr Asp His

195 200 205

Ile Lys Val Tyr Leu Ser Ser Pro His His Lys Ser Glu Ser Ile Gly

210 215 220

Ala Thr Ser His Leu Ser Thr Trp Gln Tyr Leu Tyr Ser Ser Ala Ser

225 230 235 240

Ser Lys Ile Ile Ser Met Leu Ile Leu Tyr Pro Thr Gln Val Val Arg

245 250 255

Ser Arg Leu Gln Tyr Ser Gln Asp Ser Ser Ser Ser Ile Val Ser Ile

260 265 270

Val Lys Glu Leu Tyr Tyr Lys Glu Gly Gly Leu Lys Gly Phe Tyr Lys

275 280 285

Gly Ile Gly Ala Asn Ile Leu Arg Val Leu Pro Ala Thr Cys Val Thr

290 295 300

Phe Val Ala Tyr Glu Asn Val Lys Arg Tyr Leu Met

305 310 315

<210> 16

<211> 951

<212> DNA

<213> 白色念珠菌(Candida albicans)

<400> 16

atggagttga acacaaccga caagcaagtg catactcgcc gtttttcttc acgtgaaatt 60

gaagttatat caggcttgct tgctggattc tcaacaacaa tcgtcaccca ccccctagat 120

gttatcaaga tcagattaca attatctcga gatacaccca aaactacgca tcctttggaa 180

tcaataatat ctgtgatcaa aaagataaac caagatgcaa aagtagcata caaattgaat 240

cacaagccca aggcattcaa ttatttgatc cagtactatc gaggaatcac tccaaacttg 300

atcggaaaca tttctgcttg gggtatctat tttgccttgt atgccgaatt taaaagtaaa 360

gttaagacaa acaatactac aatgaattat tttgcctcgt cggtattagc ggggttatcg 420

acgtccatta taactaatcc attatgggtg ttgaaaacac gaatattagg aagttcaaga 480

aatgaatcca atgcttatag atcagttaca gatggcataa gacaaatgct agcaaaagaa 540

ggaataacca gcttctggaa aggaaccatt cccagtcttt ttctggttgt acaagcaagt 600

ttacaaatta ccatctatga ccatatcaaa gtgtatttac tgagtcctca tcataagtca 660

gaaagtattg gtgcaacgtc tcatttgtca acttggcagt acttgtattc ttcagcatcg 720

tcgaaaatca ttagtatgtt gattctatat cccacccagg tcgttagatc acggctacag 780

tattcccaag actcttcact gagtattgtt tccatagtta aagagttgta ctataaagaa 840

ggaggattaa aggggttcta caaaggaata ggtgcaaata ttctaagagt tctacctgca 900

acgtgtgtta cttttgttgc ttatgagaac gtcaaaagat atcttatgta a 951

<210> 17

<211> 306

<212> PRT

<213> 解脂耶氏酵母(Yarrowia lipolytica)

<400> 17

Met Ser Ser Gln Asn Ser His Asn Ile Leu His Glu Thr His Leu Leu

1 5 10 15

Ser Asp Thr Ser Ile His Ala Ile Ala Gly Ala Leu Ala Gly Thr Leu

20 25 30

Ser Gly Ile Val Val Cys Pro Leu Asp Val Ile Lys Thr Arg Leu Gln

35 40 45

Ala Glu Gly Ala Leu Asp Lys Gln Arg Gly Ser Leu Lys Gly Gly Leu

50 55 60

Thr Arg Thr Met Asp Ser Ile Val Lys His Asp Gly Val Arg Gly Leu

65 70 75 80

Tyr Arg Gly Val Ile Pro Ile Ile Leu Gly Tyr Ser Pro Thr Trp Met

85 90 95

Ile Tyr Phe Ala Val Tyr Glu Lys Ser Lys Tyr Leu Leu Ser Thr Val

100 105 110

Pro Gln Leu Asp Pro Tyr Pro Phe Phe Ser His Cys Leu Ser Ala Leu

115 120 125

Gly Ala Gly Ala Ala Ser Thr Thr Ile Thr Asn Pro Ile Trp Val Val

130 135 140

Lys Thr Arg Leu Met Ser Gln Gly Arg Asn Thr Pro Trp His Tyr Ser

145 150 155 160

Gly Thr Trp Asp Ala Phe Lys Thr Met Tyr Lys Thr Asp Gly Ile Lys

165 170 175

Val Phe Tyr Ser Gly Leu Gly Pro Ala Leu Leu Gly Leu Ser His Val

180 185 190

Ala Ile Gln Phe Pro Met Tyr Glu Lys Leu Lys Val Met Leu Gly Val

195 200 205

Ser Pro Asp Ser Asn Lys Pro Asn Pro Trp Ala Val Thr Val Ala Ser

210 215 220

Ser Leu Ser Lys Met Ile Ala Ser Ala Ile Thr Tyr Pro His Glu Ile

225 230 235 240

Val Arg Thr Arg Met Gln Ile Gln Ser Lys Asp Gly Gln Tyr Arg Gly

245 250 255

Ile Ile Ala Ser Phe Lys Lys Leu Tyr Gln Glu Glu Gly Phe Arg Ile

260 265 270

Phe Tyr Thr Gly Phe Gly Thr Asn Leu Leu Arg Thr Val Pro Ala Ser

275 280 285

Ala Ile Thr Leu Leu Ser Phe Glu Met Ile Ser Ser Arg Leu Lys Gln

290 295 300

Ile Leu

305

<210> 18

<211> 921

<212> DNA

<213> 解脂耶氏酵母(Yarrowia lipolytica)

<400> 18

atgtcgtccc aaaactcgca caacatcctc catgagacgc atctgttgtc ggacaccagc 60

atccacgcca tagccggcgc cctggcaggc acgctctctg gtatcgtggt gtgtcctctc 120

gacgtgatca agacccggtt gcaggcagaa ggagctctcg acaagcagag aggctcactc 180

aagggcggcc tcacccgtac aatggactcc attgtcaagc atgacggcgt gcggggcctg 240

taccgaggag tgatccccat cattctgggc tactcgccca cctggatgat ttattttgcc 300

gtctacgaaa aatcaaaata tttattgtcc accgtgcccc agttggaccc ctatcccttc 360

ttttcgcatt gtctgtcggc tctgggcgcc ggagccgcct ccaccaccat caccaacccc 420

atctgggtcg tcaaaaccag actcatgtcc cagggccgca acaccccctg gcactactct 480

ggcacctggg acgccttcaa aaccatgtac aaaaccgacg gaatcaaagt cttctactcg 540

ggtctgggcc ccgctcttct gggcctctcc cacgtggcca ttcagttccc catgtacgag 600

aagctcaagg tgatgctcgg agtcagtcca gactccaaca aacccaaccc ctgggcagtc 660

acggtggcct cgtcactgtc caagatgatc gcgtcggcaa tcacctaccc acacgagatt 720

gtgcggaccc gaatgcagat ccagagtaaa gacggccagt atcgtggcat cattgcatcg 780

ttcaagaaac tctaccagga agagggcttc cgaatcttct acacgggctt cggcaccaac 840

ctgcttagaa ccgtgcccgc ctctgccatc accctgcttt cgtttgagat gatttccagc 900

cgcctcaaac agatccttta g 921

<210> 19

<211> 431

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 19

Met Ala Ser Ser Ile Ser Ala Ala Lys Thr Asp Asn Cys Gly Thr Asp

1 5 10 15

Asn Ile Ala Thr Gly Arg Ser Met Gly Gln Gly Leu Val Ala Glu Glu

20 25 30

Glu Ile Asp Lys Val Leu Lys Gln Asp Val Gly Asp Ala Val Thr Ala

35 40 45

Thr Ala Gly Thr Ala Ala Val Ala Ala Val Ala Ser Gly Ser Val Thr

50 55 60

Val Ser Ser Gly Val Asn Ser Gly Cys Ser Ser Arg Ser Gly Ala Ile

65 70 75 80

Asp Leu Glu Gly Ser Ser Ser Arg His Gly Leu Ser Ile Val Arg Glu

85 90 95

Glu Gly His Phe Asn Asp Thr Glu Ile Thr Ala Leu Ser Gly Ala Leu

100 105 110

Ala Gly Phe Leu Ala Gly Val Ile Val Cys Pro Leu Asp Val Ala Lys

115 120 125

Thr Arg Leu Gln Ala Gln Gly Leu Gln Leu Asn Gly Pro Val Thr Arg

130 135 140

Pro Val Gly Ser Val Ala Thr Thr Phe Gly Gly Lys Tyr Tyr Ser Gly

145 150 155 160

Ile Trp Gly Thr Leu Thr Thr Ile Val Arg Asp Glu Ser Ile Arg Gly

165 170 175

Leu Tyr Lys Gly Ile Val Pro Ile Val Leu Gly Tyr Phe Pro Thr Trp

180 185 190

Met Ile Tyr Phe Ser Val Tyr Glu Arg Cys Lys Leu Ser Tyr Pro Arg

195 200 205

Tyr Phe Asn Asn Ser Glu Phe Leu Ser His Ser Met Ser Ala Leu Thr

210 215 220

Ala Gly Ala Ile Ser Thr Thr Leu Thr Asn Pro Ile Trp Val Val Lys

225 230 235 240

Thr Arg Leu Met Leu Gln Ser Gly Lys Asn Ile Lys Gly Met Thr His

245 250 255

Tyr Lys Asn Thr Leu Asp Ala Phe Ile Lys Ile Tyr Lys Val Glu Gly

260 265 270

Ile Lys Ser Phe Tyr Ser Gly Leu Ile Pro Ser Leu Phe Gly Leu Leu

275 280 285

His Val Ala Ile His Phe Pro Val Tyr Glu Lys Leu Lys Lys Val Leu

290 295 300

His Cys Tyr Pro Ser Gly Arg Pro Asn Gln Glu Thr Met Asn Val Asn

305 310 315 320

Gly Asn Ser Asn Pro Gln Thr Thr Gly Ser Thr Asn Phe Gln Leu Gly

325 330 335

Arg Leu Ile Val Ala Ser Cys Gly Ser Lys Met Ile Ala Ser Thr Leu

340 345 350

Thr Tyr Pro His Glu Ile Leu Arg Thr Arg Leu Gln Leu Lys Ser Asp

355 360 365

Met Lys Pro Ser Ile Lys Ser Ile Ile Arg Thr Thr Tyr Ala Lys Glu

370 375 380

Gly Ile Arg Gly Phe Tyr Ser Gly Phe Leu Thr Asn Met Phe Arg Thr

385 390 395 400

Val Pro Ala Ser Ala Ile Thr Leu Val Ser Phe Glu Tyr Phe Arg Lys

405 410 415

His Phe Lys Leu Trp Asn Asp Ser Ile Glu Val Glu Arg Gly Gly

420 425 430

<210> 20

<211> 1296

<212> DNA

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 20

atggcatctt cgatcagtgc tgcaaaaact gacaattgtg gtactgataa tatagcaact 60

ggacggtcga tgggtcaagg attggttgcg gaggaggaga tagataaagt actcaagcag 120

gatgtcggag atgcagtgac agcaacagct ggcacggcag ctgtcgctgc cgtagcgtct 180

ggttctgtca ctgtcagttc gggcgtgaac tccggttgta gttctagatc cggcgctata 240

gacttggaag gttcttcgag ccgacatgga cttagtattg taagggaaga ggggcatttc 300

aacgatacag aaattacagc attgagtggt gcattggctg ggttcttggc cggtgtcatc 360

gtatgtccgt tggatgttgc caagactagg ttacaggctc aaggtttgca acttaatggt 420

ccggtgactc gtccggtcgg tagtgttgct accacgtttg gagggaaata ttacagtggt 480

atctggggaa ctctcacgac gattgtacga gacgaatcca ttagaggatt gtacaagggg 540

atagtaccga tagttctggg gtacttcccg acatggatga tatatttttc agtttacgaa 600

cgatgtaagc tatcgtaccc tcggtatttc aacaattcgg agtttctttc gcattcgatg 660

tctgctttga ctgcaggagc catttccaca acgctcacga acccgatctg ggtcgttaag 720

acacggttaa tgcttcagtc aggtaagaat attaagggca tgacgcatta caaaaacacc 780

ttggacgcat ttattaagat atacaaagtc gaaggaatca agagttttta ctccgggttg 840

atcccatcgt tgtttggatt attacatgta gctatacact tccccgtata cgaaaaactg 900

aagaaggttt tgcattgtta cccaagtggt agacctaatc aggaaactat gaacgtcaac 960

ggtaacagta acccacagac taccggttct acaaattttc agttgggacg gttgatcgtt 1020

gcatcctgtg ggtcaaagat gatcgcttca acgctcacct accctcatga aatactaaga 1080

accaggttac aactgaaatc tgatatgaaa ccttccatta aatcgataat acgaacgact 1140

tatgcaaagg aagggataag agggttttat tcgggtttcc taactaatat gttcagaact 1200

gtaccggcat cagcaattac tttagtctcg tttgaatact tcaggaagca tttcaaactt 1260

tggaacgata gtatagaggt agaaaggggc ggatag 1296

<210> 21

<211> 285

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 21

Met Lys Ala Asp Asn Pro Phe Asp Leu Leu Leu Pro Ala Ala Met Ala

1 5 10 15

Lys Val Ala Glu Glu Ala Gly Val Tyr Lys Ala Thr Lys His Pro Leu

20 25 30

Lys Thr Phe Tyr Leu Ala Ile Thr Ala Gly Val Phe Ile Ser Ile Ala

35 40 45

Phe Val Phe Tyr Ile Thr Ala Thr Thr Gly Thr Gly Thr Met Pro Phe

50 55 60

Gly Met Ala Lys Leu Val Gly Gly Ile Cys Phe Ser Leu Gly Leu Ile

65 70 75 80

Leu Cys Val Val Cys Gly Ala Asp Leu Phe Thr Ser Thr Val Leu Ile

85 90 95

Val Val Ala Lys Ala Ser Gly Arg Ile Thr Trp Gly Gln Leu Ala Lys

100 105 110

Asn Trp Leu Asn Val Tyr Phe Gly Asn Leu Val Gly Ala Leu Leu Phe

115 120 125

Val Leu Leu Met Trp Leu Ser Gly Glu Tyr Met Thr Ala Asn Gly Gln

130 135 140

Trp Gly Leu Asn Val Leu Gln Thr Ala Asp His Lys Val His His Thr

145 150 155 160

Phe Ile Glu Ala Val Cys Leu Gly Ile Leu Ala Asn Leu Met Val Cys

165 170 175

Leu Ala Val Trp Met Ser Tyr Ser Gly Arg Ser Leu Met Asp Lys Ala

180 185 190

Phe Ile Met Val Leu Pro Val Ala Met Phe Val Ala Ser Gly Phe Glu

195 200 205

His Ser Ile Ala Asn Met Phe Met Ile Pro Met Gly Ile Val Ile Arg

210 215 220

Asp Phe Ala Ser Pro Glu Phe Trp Thr Ala Val Gly Ser Ala Pro Glu

225 230 235 240

Asn Phe Ser His Leu Thr Val Met Asn Phe Ile Thr Asp Asn Leu Ile

245 250 255

Pro Val Thr Ile Gly Asn Ile Ile Gly Gly Gly Leu Leu Val Gly Leu

260 265 270

Thr Tyr Trp Val Ile Tyr Leu Arg Glu Asn Asp His His

275 280 285

<210> 22

<211> 858

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 22

gtgaaagctg acaacccttt tgatctttta cttcctgctg caatggccaa agtggccgaa 60

gaggcgggtg tctataaagc aacgaaacat ccgcttaaga ctttctatct ggcgattacc 120

gccggtgttt tcatctcaat cgcattcgtc ttctatatca cagcaaccac tggcacaggc 180

acaatgccct tcggcatggc aaaactggtt ggcggcattt gcttctctct ggggctgatt 240

ctttgtgttg tctgcggagc cgatctcttt acttccaccg tgttgattgt tgttgctaag 300

gcgagtgggc gcatcacctg gggtcagttg gcgaaaaact ggctaaatgt ctattttggc 360

aacctggtcg gcgcactgct gtttgtactt ttaatgtggc tttccggcga gtatatgacc 420

gcaaatggtc aatggggact aaacgtccta caaaccgccg accacaaagt gcaccatact 480

tttattgagg ccgtctgtct tggtatcctg gcaaacctga tggtatgtct ggcagtatgg 540

atgagttatt ctggccgcag cctgatggac aaagcgttca ttatggtgct gccggtcgcg 600

atgtttgttg ccagcggttt tgagcacagt atcgcaaaca tgtttatgat cccgatgggt 660

attgtaatcc gcgacttcgc atccccggaa ttttggaccg cagtcggttc tgcaccggaa 720

aatttttctc acctgaccgt gatgaatttc atcactgata acctgattcc ggttacgatc 780

ggcaacatta tcggtggtgg tttgttggtt gggttgacat actgggtcat ttacctgcgt 840

gaaaacgacc accattaa 858

<210> 23

<211> 616

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 23

Met Ser Ser Ser Ile Thr Asp Glu Lys Ile Ser Gly Glu Gln Gln Gln

1 5 10 15

Pro Ala Gly Arg Lys Leu Tyr Tyr Asn Thr Ser Thr Phe Ala Glu Pro

20 25 30

Pro Leu Val Asp Gly Glu Gly Asn Pro Ile Asn Tyr Glu Pro Glu Val

35 40 45

Tyr Asn Pro Asp His Glu Lys Leu Tyr His Asn Pro Ser Leu Pro Ala

50 55 60

Gln Ser Ile Gln Asp Thr Arg Asp Asp Glu Leu Leu Glu Arg Val Tyr

65 70 75 80

Ser Gln Asp Gln Gly Val Glu Tyr Glu Glu Asp Glu Glu Asp Lys Pro

85 90 95

Asn Leu Ser Ala Ala Ser Ile Lys Ser Tyr Ala Leu Thr Arg Phe Thr

100 105 110

Ser Leu Leu His Ile His Glu Phe Ser Trp Glu Asn Val Asn Pro Ile

115 120 125

Pro Glu Leu Arg Lys Met Thr Trp Gln Asn Trp Asn Tyr Phe Phe Met

130 135 140

Gly Tyr Phe Ala Trp Leu Ser Ala Ala Trp Ala Phe Phe Cys Val Ser

145 150 155 160

Val Ser Val Ala Pro Leu Ala Glu Leu Tyr Asp Arg Pro Thr Lys Asp

165 170 175

Ile Thr Trp Gly Leu Gly Leu Val Leu Phe Val Arg Ser Ala Gly Ala

180 185 190

Val Ile Phe Gly Leu Trp Thr Asp Lys Ser Ser Arg Lys Trp Pro Tyr

195 200 205

Ile Thr Cys Leu Phe Leu Phe Val Ile Ala Gln Leu Cys Thr Pro Trp

210 215 220

Cys Asp Thr Tyr Glu Lys Phe Leu Gly Val Arg Trp Ile Thr Gly Ile

225 230 235 240

Ala Met Gly Gly Ile Tyr Gly Cys Ala Ser Ala Thr Ala Ile Glu Asp

245 250 255

Ala Pro Val Lys Ala Arg Ser Phe Leu Ser Gly Leu Phe Phe Ser Ala

260 265 270

Tyr Ala Met Gly Phe Ile Phe Ala Ile Ile Phe Tyr Arg Ala Phe Gly

275 280 285

Tyr Phe Arg Asp Asp Gly Trp Lys Ile Leu Phe Trp Phe Ser Ile Phe

290 295 300

Leu Pro Ile Leu Leu Ile Phe Trp Arg Leu Leu Trp Pro Glu Thr Lys

305 310 315 320

Tyr Phe Thr Lys Val Leu Lys Ala Arg Lys Leu Ile Leu Ser Asp Ala

325 330 335

Val Lys Ala Asn Gly Gly Glu Pro Leu Pro Lys Ala Asn Phe Lys Gln

340 345 350

Lys Met Val Ser Met Lys Arg Thr Val Gln Lys Tyr Trp Leu Leu Phe

355 360 365

Ala Tyr Leu Val Val Leu Leu Val Gly Pro Asn Tyr Leu Thr His Ala

370 375 380

Ser Gln Asp Leu Leu Pro Thr Met Leu Arg Ala Gln Leu Gly Leu Ser

385 390 395 400

Lys Asp Ala Val Thr Val Ile Val Val Val Thr Asn Ile Gly Ala Ile

405 410 415

Cys Gly Gly Met Ile Phe Gly Gln Phe Met Glu Val Thr Gly Arg Arg

420 425 430

Leu Gly Leu Leu Ile Ala Cys Thr Met Gly Gly Cys Phe Thr Tyr Pro

435 440 445

Ala Phe Met Leu Arg Ser Glu Lys Ala Ile Leu Gly Ala Gly Phe Met

450 455 460

Leu Tyr Phe Cys Val Phe Gly Val Trp Gly Ile Leu Pro Ile His Leu

465 470 475 480

Ala Glu Leu Ala Pro Ala Asp Ala Arg Ala Leu Val Ala Gly Leu Ser

485 490 495

Tyr Gln Leu Gly Asn Leu Ala Ser Ala Ala Ala Ser Thr Ile Glu Thr

500 505 510

Gln Leu Ala Asp Arg Tyr Pro Leu Glu Arg Asp Ala Ser Gly Ala Val

515 520 525

Ile Lys Glu Asp Tyr Ala Lys Val Met Ala Ile Leu Thr Gly Ser Val

530 535 540

Phe Ile Phe Thr Phe Ala Cys Val Phe Val Gly His Glu Lys Phe His

545 550 555 560

Arg Asp Leu Ser Ser Pro Val Met Lys Lys Tyr Ile Asn Gln Val Glu

565 570 575

Glu Tyr Glu Ala Asp Gly Leu Ser Ile Ser Asp Ile Val Glu Gln Lys

580 585 590

Thr Glu Cys Ala Ser Val Lys Met Ile Asp Ser Asn Val Ser Lys Thr

595 600 605

Tyr Glu Glu His Ile Glu Thr Val

610 615

<210> 24

<211> 1851

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 24

atgtcgtcgt caattacaga tgagaaaata tctggtgaac agcaacaacc tgctggcaga 60

aaactatact ataacacaag tacatttgca gagcctcctc tagtggacgg agaaggtaac 120

cctataaatt atgagccgga agtttacaac ccggatcacg aaaagctata ccataaccca 180

tcactgcctg cacaatcaat tcaggataca agagatgatg aattgctgga aagagtttat 240

agccaggatc aaggtgtaga gtatgaggaa gatgaagagg ataagccaaa cctaagcgct 300

gcgtccatta aaagttatgc tttaacgaga tttacgtcct tactgcacat ccacgagttt 360

tcttgggaga atgtcaatcc catacccgaa ctgcgcaaaa tgacatggca gaattggaac 420

tattttttta tgggttattt tgcgtggttg tctgcggctt gggccttctt ttgcgtttca 480

gtatcagtcg ctccattggc tgaactatat gacagaccaa ccaaggacat cacctggggg 540

ttgggattgg tgttatttgt tcgttcagca ggtgctgtca tatttggttt atggacagat 600

aagtcttcca gaaagtggcc gtacattaca tgtttgttct tatttgtcat tgcacaactc 660

tgtactccat ggtgtgacac atacgagaaa tttctgggcg taaggtggat aaccggtatt 720

gctatgggag gaatttacgg atgtgcttct gcaacagcga ttgaagatgc acctgtgaaa 780

gcacgttcgt tcctatcagg tctatttttt tctgcttacg ctatggggtt catatttgct 840

atcatttttt acagagcctt tggctacttt agggatgatg gctggaaaat attgttttgg 900

tttagtattt ttctaccaat tctactaatt ttctggagat tgttatggcc tgaaacgaaa 960

tacttcacca aggttttgaa agcccgtaaa ttaatattga gtgacgcagt gaaagctaat 1020

ggtggcgagc ctctaccaaa agccaacttt aaacaaaaga tggtatccat gaagagaaca 1080

gttcaaaagt actggttgtt gttcgcatat ttggttgttt tattggtggg tccaaattac 1140

ttgactcatg cttctcaaga cttgttgcca accatgctgc gtgcccaatt aggcctatcc 1200

aaggatgctg tcactgtcat tgtagtggtt accaacatcg gtgctatttg tgggggtatg 1260

atatttggac agttcatgga agttactgga agaagattag gcctattgat tgcatgcaca 1320

atgggtggtt gcttcaccta ccctgcattt atgttgagaa gcgaaaaggc tatattaggt 1380

gccggtttca tgttatattt ttgtgtcttt ggtgtctggg gtatcctgcc cattcacctt 1440

gcagagttgg cccctgctga tgcaagggct ttggttgccg gtttatctta ccagctaggt 1500

aatctagctt ctgcagcggc ttccacgatt gagacacagt tagctgatag atacccatta 1560

gaaagagatg cctctggtgc tgtgattaaa gaagattatg ccaaagttat ggctatcttg 1620

actggttctg ttttcatctt cacatttgct tgtgtttttg ttggccatga gaaattccat 1680

cgtgatttgt cctctcctgt tatgaagaaa tatataaacc aagtggaaga atacgaagcc 1740

gatggtcttt cgattagtga cattgttgaa caaaagacgg aatgtgcttc agtgaagatg 1800

attgattcga acgtctcaaa gacatatgag gagcatattg agaccgttta a 1851

<210> 25

<211> 440

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 25

Met Gln Ala Thr Ala Thr Thr Leu Asp His Glu Gln Glu Tyr Thr Pro

1 5 10 15

Ile Asn Ser Arg Asn Lys Val Leu Val Ala Ser Leu Ile Gly Thr Ala

20 25 30

Ile Glu Phe Phe Asp Phe Tyr Ile Tyr Ala Thr Ala Ala Val Ile Val

35 40 45

Phe Pro His Ile Phe Phe Pro Gln Gly Asp Pro Thr Ala Ala Thr Leu

50 55 60

Gln Ser Leu Ala Thr Phe Ala Ile Ala Phe Val Ala Arg Pro Ile Gly

65 70 75 80

Ser Ala Val Phe Gly His Phe Gly Asp Arg Val Gly Arg Lys Ala Thr

85 90 95

Leu Val Ala Ser Leu Leu Thr Met Gly Ile Ser Thr Val Val Ile Gly

100 105 110

Leu Leu Pro Gly Tyr Ala Thr Ile Gly Ile Phe Ala Pro Leu Leu Leu

115 120 125

Ala Leu Ala Arg Phe Gly Gln Gly Leu Gly Leu Gly Gly Glu Trp Gly

130 135 140

Gly Ala Ala Leu Leu Ala Thr Glu Asn Ala Pro Pro Arg Lys Arg Ala

145 150 155 160

Leu Tyr Gly Ser Phe Pro Gln Leu Gly Ala Pro Ile Gly Phe Phe Phe

165 170 175

Ala Asn Gly Thr Phe Leu Leu Leu Ser Trp Leu Leu Thr Asp Glu Gln

180 185 190

Phe Met Ser Trp Gly Trp Arg Val Pro Phe Ile Phe Ser Ala Val Leu

195 200 205

Val Ile Ile Gly Leu Tyr Val Arg Val Ser Leu His Glu Ser Pro Val

210 215 220

Phe Glu Lys Val Ala Lys Ala Lys Lys Gln Val Lys Ile Pro Leu Gly

225 230 235 240

Thr Leu Leu Thr Lys His Val Arg Val Thr Val Leu Gly Thr Phe Ile

245 250 255

Met Leu Ala Thr Tyr Thr Leu Phe Tyr Ile Met Thr Val Tyr Ser Met

260 265 270

Thr Phe Ser Thr Ala Ala Ala Pro Val Gly Leu Gly Leu Pro Arg Asn

275 280 285

Glu Val Leu Trp Met Leu Met Met Ala Val Ile Gly Phe Gly Val Met

290 295 300

Val Pro Val Ala Gly Leu Leu Ala Asp Ala Phe Gly Arg Arg Lys Ser

305 310 315 320

Met Val Ile Ile Thr Thr Leu Ile Ile Leu Phe Ala Leu Phe Ala Phe

325 330 335

Asn Pro Leu Leu Gly Ser Gly Asn Pro Ile Leu Val Phe Ala Phe Leu

340 345 350

Leu Leu Gly Leu Ser Leu Met Gly Leu Thr Phe Gly Pro Met Gly Ala

355 360 365

Leu Leu Pro Glu Leu Phe Pro Thr Glu Val Arg Tyr Thr Gly Ala Ser

370 375 380

Phe Ser Tyr Asn Val Ala Ser Ile Leu Gly Ala Ser Val Ala Pro Tyr

385 390 395 400

Ile Ala Ala Trp Leu Gln Thr Asn Tyr Gly Leu Gly Ala Val Gly Leu

405 410 415

Tyr Leu Ala Ala Met Ala Gly Leu Thr Leu Ile Ala Leu Leu Leu Thr

420 425 430

His Glu Thr Arg His Gln Ser Leu

435 440

<210> 26

<211> 1323

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 26

atgcaagcaa cagccacaac actcgaccac gagcaagaat acacgccgat caactcgcgt 60

aataaagtcc ttgtcgcctc tctcattggc acagccattg agttcttcga cttttacatt 120

tacgccactg cggccgttat tgtgtttccg catatcttct tcccgcaggg cgatcctacg 180

gcagcaacgc tacagtcgct cgccaccttc gccatcgcct tcgtcgcgcg ccccattggc 240

tctgccgttt ttggtcattt tggcgatcgc gttgggcgta aagcgacgct ggtcgcctcg 300

ttgctaacga tggggatttc gaccgtggtg attggtctgc tgccgggcta tgccacgatt 360

ggtattttcg ccccgctgct gctggcgctg gctcgatttg gtcagggtct gggcttaggc 420

ggtgaatggg gcggcgcggc gctgctggcg actgaaaacg ccccaccgcg caaacgtgca 480

ctgtatggct cctttccgca gctgggcgca ccgattggct tcttctttgc caatggcact 540

ttcttgctgc tttcctggct actgaccgac gagcagttta tgagctgggg ctggcgtgtg 600

ccatttatct tctcggcggt gctggtcatt atcggcctgt atgttcgcgt gtcgctgcat 660

gagtcgccgg tgtttgagaa agtcgctaaa gcgaaaaaac aggtgaagat cccgctgggt 720

acgctgctga ccaaacatgt tcgcgtaacc gtactgggta cgttcattat gctggcaacc 780

tatacgctgt tttacatcat gacggtctac tctatgacct ttagtaccgc cgccgcgcca 840

gttgggcttg gcctgccgcg taacgaagtg ttgtggatgt tgatgatggc agttattggt 900

tttggcgtga tggtgccagt cgctggatta ctggctgatg cctttggtcg ccgtaaaagc 960

atggtaatca tcaccacgct gatcatcctg ttcgcgctgt tcgcctttaa cccactgctc 1020

ggttctggca acccgattct ggtttttgcc ttcctgctgc tggggttaag tctgatgggt 1080

ctgaccttcg ggccaatggg tgcgctgtta ccagagctgt ttccgacaga agtgcgttac 1140

accggagcat cgttctctta caacgtagcg tcgattctcg gggcttccgt tgcgccatat 1200

atcgcagcct ggttgcagac taactacggg ctaggtgcgg tggggttata tctggcggcg 1260

atggctggct tgacgttaat cgccctgctg ctgacccatg agacgcgaca tcagtcgttg 1320

taa 1323

<210> 27

<211> 410

<212> PRT

<213> 鹑鸡肠球菌(Enterococcus gallinarum)

<400> 27

Met His Gly Trp Thr Ser Arg Gln Arg Asn Ala Ala Ile Ala Ser Phe

1 5 10 15

Leu Ser Trp Thr Leu Asp Ala Phe Asp Phe Phe Leu Leu Val Phe Leu

20 25 30

Leu Ser Asp Ile Ala His Ser Phe His Val Asp Leu Glu Glu Val Thr

35 40 45

Leu Ala Ile Leu Leu Thr Leu Ala Val Arg Pro Val Gly Ala Leu Ile

50 55 60

Phe Gly Arg Ala Ala Glu Lys Phe Gly Arg Lys Pro Ile Leu Met Leu

65 70 75 80

Asn Ile Val Phe Phe Ser Ala Phe Glu Leu Leu Ser Ala Ala Ala Pro

85 90 95

Ser Leu Met Leu Phe Phe Leu Leu Arg Val Leu Tyr Gly Val Ala Met

100 105 110

Gly Gly Ile Trp Gly Val Ala Ser Ser Leu Ala Met Glu Thr Ile Pro

115 120 125

Asp Arg Ser Arg Gly Leu Met Ser Gly Leu Phe Gln Ala Gly Tyr Pro

130 135 140

Phe Gly Tyr Leu Leu Ala Ala Val Ala Tyr Gly Leu Leu Phe Glu Gln

145 150 155 160

Leu Gly Trp Arg Gly Met Phe Val Ile Gly Ala Ala Pro Val Leu Leu

165 170 175

Leu Pro Phe Ile Tyr Phe Cys Val Glu Glu Ser Pro Val Trp Gln Ala

180 185 190

Ala Arg Gln Asn Lys Glu Ser Thr Ala Leu Leu Pro Val Leu Arg Ser

195 200 205

His Trp Lys Leu Cys Leu Tyr Leu Val Val Leu Met Ala Ala Phe Asn

210 215 220

Phe Phe Ser His Gly Thr Gln Asp Leu Tyr Pro Val Phe Leu Lys Val

225 230 235 240

Gln His Gly Phe Glu Pro Lys Thr Val Ser Ile Ile Ala Val Cys Tyr

245 250 255

Asn Ile Ala Ser Ile Ile Gly Gly Val Phe Phe Gly Ser Leu Ser Glu

260 265 270

Lys Ile Gly Arg Arg Lys Ala Ile Met Ile Ala Ala Met Leu Ala Leu

275 280 285

Pro Val Ile Pro Leu Trp Ala Phe Ala Ser Gly Ser Leu Ala Leu Gly

290 295 300

Ala Gly Ala Phe Leu Met Gln Phe Met Val Gln Gly Ala Trp Gly Val

305 310 315 320

Ile Pro Thr Trp Leu Asn Glu Leu Val Pro Ala Asn Thr Arg Ala Val

325 330 335

Leu Pro Gly Phe Val Tyr Gln Leu Gly Asn Leu Leu Ala Ser Val Asn

340 345 350

Ala Thr Leu Gln Ala Ser Ile Ala Gln His His Gly His Asn Tyr Gly

355 360 365

Leu Ala Met Ala Leu Val Ala Gly Thr Val Ala Ile Val Ile Thr Val

370 375 380

Leu Thr Phe Phe Gly Arg Glu Gly Arg Val Ile Gln Ser Ala Gly Ala

385 390 395 400

Gly His His Gln Pro Leu Ser Thr Ser Arg

405 410

<210> 28

<211> 1233

<212> DNA

<213> 鹑鸡肠球菌(Enterococcus gallinarum)

<400> 28

atgcacggct ggacctcacg acagcgtaat gcggcgatag ccagtttttt aagctggacg 60

ctcgatgctt tcgacttttt cctgttagtt tttttactga gcgatatcgc ccactcgttt 120

catgtcgacc ttgaggaggt cactctggcg attctgctga ctctggccgt gcggcccgtc 180

ggcgcgctaa ttttcggcag ggcggcggag aagtttggtc gcaaaccaat cctgatgctc 240

aacattgtgt tcttctcggc ttttgagctg ctctccgccg ccgcgccgtc gttgatgctg 300

tttttcctgc tgcgggtgct gtacggcgtg gcgatgggcg ggatctgggg cgtggcctct 360

tcgctggcga tggagacgat ccccgaccgc tcgcgcggcc tgatgtccgg cctgttccag 420

gcgggatacc ccttcggcta tttgctggcg gccgtcgcct acgggctgct gtttgaacaa 480

ctcggctggc gcgggatgtt tgtcattggc gccgcgccgg tgcttctgct gccgtttatc 540

tatttctgcg tcgaggagtc tccggtttgg caggcggcca ggcagaataa agagagtacg 600

gccctgctgc cggtactgcg tagccactgg aagctgtgcc tgtacctggt ggtgctgatg 660

gcggctttca acttcttctc tcacgggacg caggatcttt acccggtctt tttgaaagtt 720

cagcacggct tcgagcctaa aacggtcagc atcatcgcgg tctgctataa catcgcctcg 780

atcattggtg gagtgttctt cggttcgctg tcggagaaga ttggccgacg caaagcgatt 840

atgatcgccg ccatgctggc gctgccggtc attccactgt gggctttcgc cagcggctcg 900

ctggcgctgg gggcgggggc gttcctgatg cagtttatgg tccagggggc ctggggggtg 960

atccccacct ggctcaatga actggtcccg gccaacaccc gggcggtgct gcccggcttc 1020

gtctaccagc tgggtaattt gctggcttcg gtgaatgcca ctctccaggc atcaatcgcc 1080

cagcatcatg ggcataacta cggcctggca atggccctgg tggccgggac ggtggcgatt 1140

gtcattaccg tcctgacctt ttttggtcgc gaaggccggg tgatccaatc cgcaggagcg 1200

ggacatcacc agccgctctc caccagccgt taa 1233

<210> 29

<211> 419

<212> PRT

<213> 杨氏脱硫芽孢弯曲菌(Desulfosporosinus youngiae)

<400> 29

Met Ala Glu Leu Ser Tyr Asn Gln Lys Ile Ile Ala Asp Pro Leu Lys

1 5 10 15

Tyr Lys Tyr Lys Ala Leu Thr Gly Ala Ile Leu Gly Tyr Met Phe Asp

20 25 30

Ala Gln Asp Phe Met Val Leu Ala Leu Val Ile Pro Leu Leu Val Lys

35 40 45

Thr Trp Gly Ile Ser Leu Ala Ser Ala Gly Leu Ile Ser Thr Ala Thr

50 55 60

Ile Phe Gly Ala Ala Leu Ser Gly Tyr Leu Trp Gly Pro Met Ile Asp

65 70 75 80

Lys Phe Gly Arg Lys Lys Met Leu Ile Leu Cys Leu Ala Trp Phe Gly

85 90 95

Ile Phe Thr Phe Leu Cys Gly Phe Ala Thr Asn Tyr Thr Gln Leu Ile

100 105 110

Leu Leu Arg Phe Ile Ala Gly Ile Gly Leu Gly Gly Glu Trp Val Ile

115 120 125

Gly Ala Ala Leu Ile Ser Glu Phe Phe Pro Pro Glu Gln Arg Ala Arg

130 135 140

Ala Thr Ser Ala Val Gln Ser Gly Trp Pro Leu Gly Tyr Ala Leu Ala

145 150 155 160

Leu Gly Val Asn Ala Tyr Leu Val Pro Thr Tyr Gly Trp Gln Ile Leu

165 170 175

Phe Phe Ser Gly Ile Leu Ser Leu Ile Ala Ala Val Tyr Ile Ala Val

180 185 190

Phe Val Pro Glu Ser Pro Ala Trp Leu Lys Ala Gln Ile Asn Lys Ser

195 200 205

Gln Gly Lys Glu Ser Val Ser Lys Ala Glu Val Lys Ala Ala Thr Trp

210 215 220

Thr Asp Leu Leu Lys Gly Ala Asn Leu Lys Thr Thr Leu Leu Ala Phe

225 230 235 240

Gly Leu Cys Ala Ser Cys Leu Val Ser Tyr Trp Gly Ala Gly Ser Trp

245 250 255

Ile Pro Ala Tyr Leu Ser Ala Glu Arg Gly Leu Asn Val Lys Asp Met

260 265 270

Ser Gly Tyr Leu Met Ile Leu Asn Val Gly Gly Phe Ile Gly Tyr Tyr

275 280 285

Val Tyr Gly Tyr Phe Ala Asp Lys Val Gly Arg Arg Ala Asn Phe Ile

290 295 300

Phe Gly Ser Leu Ala Ser Ala Ala Val Met Leu Ile Trp Ile Asn Leu

305 310 315 320

Ser Ser Pro Thr Ala Ile Leu Trp Met Ala Gly Val Phe Gly Phe Ile

325 330 335

Thr Tyr Gly Tyr Trp Gly Pro Leu Ala Ala Phe Val Ser Glu Gln Phe

340 345 350

Pro Thr Asn Val Arg Gly Ile Gly Thr Ala Phe Ala Tyr Ala Ser Gly

355 360 365

Arg Met Met Ser Ala Leu Ala Pro Phe Leu Met Gly Gly Ile Ala Ser

370 375 380

Lys Tyr Ser Leu Gly Phe Ala Leu Gly Leu Val Ser Val Ile Tyr Ala

385 390 395 400

Ala Gly Ala Ile Phe Gly Tyr Phe Met Lys Glu Thr Lys Asp Ile Ile

405 410 415

Ile Val Asp

<210> 30

<211> 1260

<212> DNA

<213> 杨氏脱硫芽孢弯曲菌(Desulfosporosinus youngiae)

<400> 30

atggcggaat tatcctataa ccaaaaaata atagctgatc ctcttaagta taagtataag 60

gccttaaccg gtgccatatt gggttatatg ttcgatgctc aagactttat ggttctggcc 120

ctggttattc ctttgctggt aaaaacctgg ggcattagtt tagccagtgc cgggttaata 180

agtacagcga ctatctttgg cgcggcctta agcggctatc tttggggacc aatgatcgat 240

aaatttggac gcaagaagat gctgatcctg tgtctggcct ggtttggtat tttcaccttc 300

ttgtgtggat tcgcgacaaa ttatacgcaa ttaatcttat taagatttat agccggtata 360

gggctggggg gagaatgggt tattggtgcg gctttaatca gtgaattctt ccctcctgaa 420

caaagggctc gggctacttc ggcagtgcag agcggctggc ccctgggcta tgctctggct 480

cttggcgtaa atgcctattt ggttccaacc tatggttggc agatcctttt cttttcagga 540

attctttctt tgattgcagc agtctatatc gctgtatttg ttccggagtc accagcctgg 600

ttaaaagccc aaatcaacaa aagtcaaggc aaagaatctg tctccaaagc ggaggttaag 660

gcggcaacct ggactgatct gctcaaagga gccaacctga aaaccacctt attggctttc 720

ggactttgcg caagctgtct ggtctcttac tggggagcgg gatcgtggat tccggcctac 780

ctttcggcag aacggggcct gaatgtaaaa gacatgagcg gttatttgat gattttaaat 840

gtggggggct ttatcggtta ttatgtttat ggatattttg cggataaagt cggacgtcgg 900

gcaaacttta tttttggatc actggcctcg gcggcagtta tgctgatctg gatcaacctc 960

agcagcccaa cagctatctt gtggatggca ggggtatttg gttttataac ctacggctat 1020

tggggaccac tggcagcctt tgtttctgaa caatttccca ccaatgtgcg cgggatcgga 1080

acagcttttg cctatgccag cggcagaatg atgtccgcct tagctccttt tttgatgggt 1140

ggcattgcca gcaaatacag cctgggtttt gccttgggat tagtatccgt gatctatgcg 1200

gcaggtgcaa tcttcggata ctttatgaaa gaaacgaaag atatcattat agttgactag 1260

<210> 31

<211> 410

<212> PRT

<213> 肺炎克雷伯氏菌(Klebsiella pneumoniae)

<400> 31

Met His Gly Trp Thr Ser Arg Gln Arg Asn Ala Ala Ile Ala Ser Phe

1 5 10 15

Leu Ser Trp Thr Leu Asp Ala Phe Asp Phe Phe Leu Leu Val Phe Leu

20 25 30

Leu Ser Asp Ile Ala His Ser Phe His Val Asp Leu Glu Glu Val Thr

35 40 45

Leu Ala Ile Leu Leu Thr Leu Ala Val Arg Pro Val Gly Ala Leu Ile

50 55 60

Phe Gly Arg Ala Ala Glu Lys Phe Gly Arg Lys Pro Ile Leu Met Leu

65 70 75 80

Asn Ile Val Phe Phe Ser Ala Phe Glu Leu Leu Ser Ala Ala Ala Pro

85 90 95

Ser Leu Met Leu Phe Phe Leu Leu Arg Val Leu Tyr Gly Val Ala Met

100 105 110

Gly Gly Ile Trp Gly Val Ala Ser Ser Leu Ala Met Glu Thr Ile Pro

115 120 125

Asp Arg Ser Arg Gly Leu Met Ser Gly Leu Phe Gln Ala Gly Tyr Pro

130 135 140

Phe Gly Tyr Leu Leu Ala Ala Val Ala Tyr Gly Leu Phe Phe Glu Gln

145 150 155 160

Leu Gly Trp Arg Gly Met Phe Val Ile Gly Ala Ala Pro Val Leu Leu

165 170 175

Leu Pro Phe Ile Tyr Phe Cys Val Glu Glu Ser Pro Val Trp Gln Ala

180 185 190

Ala Arg Gln Asn Lys Glu Ser Thr Ser Leu Leu Pro Val Leu Arg Ser

195 200 205

His Trp Lys Leu Cys Leu Tyr Leu Val Val Leu Met Ala Ala Phe Asn

210 215 220

Phe Phe Ser His Gly Thr Gln Asp Leu Tyr Pro Val Phe Leu Lys Val

225 230 235 240

Gln His Gly Phe Glu Pro Lys Thr Val Ser Ile Ile Ala Val Cys Tyr

245 250 255

Asn Ile Ala Ser Ile Ile Gly Gly Val Phe Phe Gly Ser Leu Ser Glu

260 265 270

Lys Ile Gly Arg Arg Lys Ala Ile Met Ile Ala Ala Leu Leu Ala Leu

275 280 285

Pro Val Ile Pro Leu Trp Ala Phe Ala Ser Gly Ser Leu Ala Leu Gly

290 295 300

Ala Gly Ala Phe Leu Met Gln Phe Met Val Gln Gly Ala Trp Gly Val

305 310 315 320

Ile Pro Thr Trp Leu Asn Glu Leu Val Pro Ala Asn Thr Arg Ala Val

325 330 335

Leu Pro Gly Phe Val Tyr Gln Leu Gly Asn Leu Leu Ala Ser Val Asn

340 345 350

Ala Thr Leu Gln Ala Ser Ile Ala Gln His His Gly His Asn Tyr Gly

355 360 365

Leu Ala Met Ala Leu Val Ala Gly Thr Val Ala Ile Val Ile Thr Val

370 375 380

Leu Thr Phe Phe Gly Arg Glu Gly Arg Val Ile Gln Ser Ala Gly Ala

385 390 395 400

Gly His His Gln Pro Leu Ser Thr Ser Arg

405 410

<210> 32

<211> 1233

<212> DNA

<213> 肺炎克雷伯氏菌(Klebsiella pneumoniae)

<400> 32

atgcacggct ggacctcacg acagcgtaat gcggcgatag ccagtttttt aagctggacg 60

ctcgatgctt tcgacttttt cctgttagtt tttttactga gcgatatcgc ccactcgttt 120

catgtcgacc ttgaggaggt caccctggcg attctgctga ctctggccgt gcggcccgtc 180

ggcgcgctaa ttttcggcag ggcggcggag aagtttggtc gcaaaccaat cctgatgctc 240

aatattgtgt tcttctcggc ttttgagctg ctctccgccg ccgcgccgtc gttgatgctg 300

tttttcctgc tgcgggtgct gtacggcgtg gcgatgggcg ggatctgggg cgtggcctct 360

tcgctggcga tggagacgat ccccgaccgc tcgcgcggcc tgatgtccgg cctgttccag 420

gcgggatacc ccttcggcta tttgctggcg gccgtcgcct atgggctgtt ttttgaacaa 480

ctcggctggc gcgggatgtt tgtcattggc gccgcgccgg tgctgctgct gccgtttatc 540

tatttctgcg tcgaggagtc tccggtttgg caggcggcca ggcagaataa agagagtacg 600

tccctgctgc cggtactgcg tagccactgg aagctgtgcc tgtacctggt ggtgttgatg 660

gcggctttca acttcttctc ccacgggacg caggatcttt acccggtctt tttgaaagtt 720

cagcacggct tcgagcctaa aacggtcagc atcatcgcgg tctgctataa catcgcctcg 780

atcattggtg gagtgttctt cggttcgctg tcggagaaga ttggccgacg caaagcgatt 840

atgatcgccg ccctgctggc gctgccggtc attccactgt gggctttcgc cagcggctcg 900

ctggcgctgg gggcgggggc gttcctgatg cagtttatgg tccagggggc ctggggggtg 960

atccccacct ggctcaatga actggtcccg gccaacaccc gggcggtgct gcccggcttc 1020

gtctaccagc tgggtaattt gctggcttcg gtgaatgcca ctctccaggc atcaatcgcc 1080

cagcatcatg ggcataacta cggcctggca atggccctgg tggccgggac ggtggcgatt 1140

gtcattaccg tcctgacctt ttttggtcgc gaaggccggg tgatccaatc cgcaggagcg 1200

ggacatcacc agccgctctc caccagccgt taa 1233

<210> 33

<211> 405

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 33

Met Ala Thr Ala Trp Tyr Lys Gln Val Asn Pro Pro Gln Arg Lys Ala

1 5 10 15

Leu Phe Ser Ala Trp Leu Gly Tyr Val Phe Asp Gly Phe Asp Phe Met

20 25 30

Met Ile Phe Tyr Ile Leu His Ile Ile Lys Ala Asp Leu Gly Ile Thr

35 40 45

Asp Ile Gln Ala Thr Leu Ile Gly Thr Val Ala Phe Ile Ala Arg Pro

50 55 60

Ile Gly Gly Gly Phe Phe Gly Ala Met Ala Asp Lys Tyr Gly Arg Lys

65 70 75 80

Pro Met Met Met Trp Ala Ile Phe Ile Tyr Ser Val Gly Thr Gly Leu

85 90 95

Ser Gly Ile Ala Thr Asn Leu Tyr Met Leu Ala Val Cys Arg Phe Ile

100 105 110

Val Gly Leu Gly Met Ser Gly Glu Tyr Ala Cys Ala Ser Thr Tyr Ala

115 120 125

Val Glu Ser Trp Pro Lys Asn Leu Gln Ser Lys Ala Ser Ala Phe Leu

130 135 140

Val Ser Gly Phe Ser Val Gly Asn Ile Ile Ala Ala Gln Ile Ile Pro

145 150 155 160

Gln Phe Ala Glu Val Tyr Gly Trp Arg Asn Ser Phe Phe Ile Gly Leu

165 170 175

Leu Pro Val Leu Leu Val Leu Trp Ile Arg Lys Ser Ala Pro Glu Ser

180 185 190

Gln Glu Trp Ile Glu Asp Lys Tyr Lys Asp Lys Ser Thr Phe Leu Ser

195 200 205

Val Phe Arg Lys Pro His Leu Ser Ile Ser Met Ile Val Phe Leu Val

210 215 220

Cys Phe Cys Leu Phe Gly Ala Asn Trp Pro Ile Asn Gly Leu Leu Pro

225 230 235 240

Ser Tyr Leu Ala Asp Asn Gly Val Asn Thr Val Val Ile Ser Thr Leu

245 250 255

Met Thr Ile Ala Gly Leu Gly Thr Leu Thr Gly Thr Ile Phe Phe Gly

260 265 270

Phe Val Gly Asp Lys Ile Gly Val Lys Lys Ala Phe Val Val Gly Leu

275 280 285

Ile Thr Ser Phe Ile Phe Leu Cys Pro Leu Phe Phe Ile Ser Val Lys

290 295 300

Asn Ser Ser Leu Ile Gly Leu Cys Leu Phe Gly Leu Met Phe Thr Asn

305 310 315 320

Leu Gly Ile Ala Gly Leu Val Pro Lys Phe Ile Tyr Asp Tyr Phe Pro

325 330 335

Thr Lys Leu Arg Gly Leu Gly Thr Gly Leu Ile Tyr Asn Leu Gly Ala

340 345 350

Thr Gly Gly Met Ala Ala Pro Val Leu Ala Thr Tyr Ile Ser Gly Tyr

355 360 365

Tyr Gly Leu Gly Val Ser Leu Phe Ile Val Thr Val Ala Phe Ser Ala

370 375 380

Leu Leu Ile Leu Leu Val Gly Phe Asp Ile Pro Gly Lys Ile Tyr Lys

385 390 395 400

Leu Ser Val Ala Lys

405

<210> 34

<211> 1218

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 34

atggcaacag catggtataa acaagttaat ccaccacaac ggaaagctct tttttccgca 60

tggcttggat atgtatttga tggctttgat tttatgatga tattttacat tcttcatatt 120

ataaaagcag atcttggcat tacggatatt caggctactt taatagggac agtggccttc 180

atagccagac ctattggagg tggttttttt ggtgccatgg ctgataaata tggtcgtaag 240

ccaatgatga tgtgggcaat tttcatttac tcagtcggaa caggccttag cggtattgct 300

acaaacttat atatgctcgc agtttgccgt tttattgttg gcttagggat gtctggtgaa 360

tatgcatgtg cttcaactta tgcggtagaa agttggccta aaaatcttca atctaaagct 420

agtgcttttt tggtaagtgg tttttctgtt ggaaatatta ttgcggcaca aataatccct 480

cagtttgctg aagtatatgg atggagaaac tcttttttta taggcctgtt accagtttta 540

ctagttcttt ggatcagaaa aagtgctcca gaaagtcagg agtggattga agataaatat 600

aaggataaat caacattttt gtctgtcttc agaaaaccac atctttcaat ctctatgatc 660

gttttcctcg tctgtttttg tctatttggt gcaaactggc cgataaacgg actacttcct 720

tcctacctgg cagataatgg agttaataca gtggtcattt caactctgat gacaatagca 780

ggtttaggaa cactgacagg tacaatattt tttggttttg ttggtgataa gattggtgta 840

aaaaaagcct ttgtagtcgg tctaataact tcatttattt tcctttgtcc tctttttttt 900

atttctgtga aaaactcttc tcttatagga ttatgtctct ttggattaat gtttacaaat 960

ttaggtattg cagggttggt tccaaaattt atatatgatt actttccaac aaaattaaga 1020

ggattaggga ccggtcttat ttataactta ggggcaactg gaggaatggc cgcacctgta 1080

ttagctacat acatttcagg atattatggc ttaggtgttt cattattcat tgttacggtt 1140

gcattctctg ccttattaat tttgttagtt ggttttgata ttccaggtaa aatttataaa 1200

ctatccgtgg ctaaatga 1218

<210> 35

<211> 405

<212> PRT

<213> 肺炎克雷伯氏菌(Klebsiella pneumoniae)

<400> 35

Met Ala Thr Ala Trp Tyr Lys Gln Val Asn Pro Pro Gln Arg Lys Ala

1 5 10 15

Leu Phe Ser Ala Trp Leu Gly Tyr Val Phe Asp Gly Phe Asp Phe Met

20 25 30

Met Ile Phe Tyr Ile Leu His Ile Ile Lys Ala Asp Leu Gly Ile Thr

35 40 45

Asp Ile Gln Ala Thr Leu Ile Gly Thr Val Ala Phe Ile Ala Arg Pro

50 55 60

Ile Gly Gly Gly Phe Phe Gly Ala Met Ala Asp Lys Tyr Gly Arg Lys

65 70 75 80

Pro Met Met Met Trp Ala Ile Phe Ile Tyr Ser Val Gly Thr Gly Leu

85 90 95

Ser Gly Ile Ala Thr Asn Leu Tyr Met Leu Ala Val Cys Arg Phe Ile

100 105 110

Val Gly Leu Gly Met Ser Gly Glu Tyr Ala Cys Ala Ser Thr Tyr Ala

115 120 125

Val Glu Ser Trp Pro Lys Asn Leu Gln Ser Lys Ala Ser Ala Phe Leu

130 135 140

Val Ser Gly Phe Ser Val Gly Asn Ile Ile Ala Ala Gln Ile Ile Pro

145 150 155 160

Gln Phe Ala Glu Val Tyr Gly Trp Arg Asn Ser Phe Phe Ile Gly Leu

165 170 175

Leu Pro Val Leu Leu Val Leu Trp Ile Arg Lys Ser Ala Pro Glu Ser

180 185 190

Gln Glu Trp Ile Glu Asp Lys Tyr Lys Asp Lys Ser Thr Phe Leu Ser

195 200 205

Val Phe Arg Lys Pro His Leu Ser Ile Ser Met Ile Val Phe Leu Val

210 215 220

Cys Phe Cys Leu Phe Gly Ala Asn Trp Pro Ile Asn Gly Leu Leu Pro

225 230 235 240

Ser Tyr Leu Ala Asp Asn Gly Val Asn Thr Val Val Ile Ser Thr Leu

245 250 255

Met Thr Ile Ala Gly Leu Gly Thr Leu Thr Gly Thr Ile Phe Phe Gly

260 265 270

Phe Val Gly Asp Lys Ile Gly Val Lys Lys Ala Phe Val Val Gly Leu

275 280 285

Ile Thr Ser Phe Ile Phe Leu Cys Pro Leu Phe Phe Ile Ser Val Lys

290 295 300

Asn Ser Ser Leu Ile Gly Leu Cys Leu Phe Gly Leu Met Phe Thr Asn

305 310 315 320

Leu Gly Ile Ala Gly Leu Val Pro Lys Phe Ile Tyr Asp Tyr Phe Pro

325 330 335

Thr Lys Leu Arg Gly Leu Gly Thr Gly Leu Ile Tyr Asn Leu Gly Ala

340 345 350

Thr Gly Gly Met Ala Ala Pro Val Leu Ala Thr Tyr Ile Ser Gly Tyr

355 360 365

Tyr Gly Leu Gly Val Ser Leu Phe Ile Val Thr Val Ala Phe Ser Ala

370 375 380

Leu Leu Ile Leu Leu Val Gly Phe Asp Ile Pro Gly Lys Ile Tyr Lys

385 390 395 400

Leu Ser Val Ala Lys

405

<210> 36

<211> 1218

<212> DNA

<213> 肺炎克雷伯氏菌(Klebsiella pneumoniae)

<400> 36

atggcaacag catggtataa acaagttaat ccaccacaac ggaaagctct tttttccgca 60

tggcttggat atgtatttga tggctttgat tttatgatga tattttacat tcttcatatt 120

ataaaagcag atcttggcat tacggatatt caggctactt taatagggac agtggccttc 180

atagccagac ctattggagg tggttttttt ggtgccatgg ctgataaata tggtcgtaag 240

ccaatgatga tgtgggcaat tttcatttac tcagtcggaa caggccttag cggtattgct 300

acaaacttat atatgctcgc agtttgccgt tttattgttg gcttagggat gtctggtgaa 360

tatgcatgtg cttcaactta tgcggtagaa agttggccta aaaatcttca atctaaagct 420

agtgcttttt tggtaagtgg tttttctgtt ggaaatatta ttgcggcaca aataatccct 480

cagtttgctg aagtatatgg atggagaaac tcttttttta taggcctgtt accagtttta 540

ctagttcttt ggatcagaaa aagtgctcca gaaagtcagg agtggattga agataaatat 600

aaggataaat caacattttt gtctgtcttc agaaaaccac atctttcaat ctctatgatc 660

gttttcctcg tctgtttttg tctatttggt gcaaactggc cgataaacgg actacttcct 720

tcctacctgg cagataatgg agttaataca gtggtcattt caactctgat gacaatagca 780

ggtttaggaa cactgacagg tacaatattt tttggttttg ttggtgataa gattggtgta 840

aaaaaagcct ttgtagtcgg tctaataact tcatttattt tcctttgtcc tctttttttt 900

atttctgtga aaaactcttc tcttatagga ttatgtctct ttggattaat gtttacaaat 960

ttaggtattg cagggttggt tccaaaattt atatatgatt actttccaac aaaattaaga 1020

ggattaggga ccggtcttat ttataactta ggggcaactg gaggaatggc cgcacctgta 1080

ttagctacat acatttcagg atattatggc ttaggtgttt cattattcat tgttacggtt 1140

gcattctctg ccttattaat tttgttagtt ggttttgata ttccaggtaa aatttataaa 1200

ctatccgtgg ctaaatga 1218

<210> 37

<211> 426

<212> PRT

<213> 肠道沙门氏菌肠道亚种(Salmonella enterica subsp. Enterica)

<400> 37

Met Ile Ala Lys Phe Phe Pro Trp Tyr Ser Glu Ile Thr Arg Pro Gln

1 5 10 15

Lys Asn Ala Leu Phe Ser Ala Trp Leu Gly Tyr Val Phe Asp Gly Phe

20 25 30

Asp Phe Met Leu Ile Phe Tyr Ile Met Tyr Leu Ile Lys Ala Asp Leu

35 40 45

Gly Leu Thr Asp Met Glu Gly Ala Phe Leu Ala Thr Ala Ala Phe Ile

50 55 60

Gly Arg Pro Phe Gly Gly Ala Leu Phe Gly Leu Leu Ala Asp Lys Phe

65 70 75 80

Gly Arg Lys Pro Leu Met Met Trp Ser Ile Val Ala Tyr Ser Val Gly

85 90 95

Thr Gly Leu Ser Gly Leu Ala Ser Gly Val Ile Met Leu Thr Leu Ser

100 105 110

Arg Phe Ile Val Gly Met Gly Met Ala Gly Glu Tyr Ala Cys Ala Ser

115 120 125

Thr Tyr Ala Val Glu Ser Trp Pro Lys His Leu Lys Ser Lys Ala Ser

130 135 140

Ala Phe Leu Val Ser Gly Phe Gly Ile Gly Asn Ile Ile Ala Ala Tyr

145 150 155 160

Phe Met Pro Ser Phe Ala Glu Ala Tyr Gly Trp Arg Ala Ala Phe Phe

165 170 175

Val Gly Leu Leu Pro Val Leu Leu Val Ile Tyr Ile Arg Ala Arg Ala

180 185 190

Pro Glu Ser Lys Glu Trp Glu Glu Ala Lys Leu Ser Gly Leu Gly Lys

195 200 205

His Ser Gln Ser Ala Trp Ser Val Phe Ser Leu Ser Met Lys Gly Leu

210 215 220

Phe Asn Arg Ala Gln Phe Pro Leu Thr Leu Cys Val Phe Ile Val Leu

225 230 235 240

Phe Ser Ile Phe Gly Ala Asn Trp Pro Ile Phe Gly Leu Leu Pro Thr

245 250 255

Tyr Leu Ala Gly Glu Gly Phe Asp Thr Gly Val Val Ser Asn Leu Met

260 265 270

Thr Ala Ala Ala Phe Gly Thr Val Leu Gly Asn Ile Val Trp Gly Leu

275 280 285

Cys Ala Asp Arg Ile Gly Leu Lys Lys Thr Phe Ser Ile Gly Leu Leu

290 295 300

Met Ser Phe Leu Phe Ile Phe Pro Leu Phe Arg Ile Pro Gln Asp Asn

305 310 315 320

Tyr Leu Leu Leu Gly Ala Cys Leu Phe Gly Leu Met Ala Thr Asn Val

325 330 335

Gly Val Gly Gly Leu Val Pro Lys Phe Leu Tyr Asp Tyr Phe Pro Leu

340 345 350

Glu Val Arg Gly Leu Gly Thr Gly Leu Ile Tyr Asn Leu Ala Ala Thr

355 360 365

Ser Gly Thr Phe Asn Ser Met Ala Ala Thr Trp Leu Gly Ile Thr Met

370 375 380

Gly Leu Gly Val Ala Leu Thr Phe Ile Val Ala Phe Trp Thr Ala Thr

385 390 395 400

Ile Leu Leu Ile Ile Gly Leu Ser Ile Pro Asp Arg Leu Lys Ala Arg

405 410 415

Arg Glu Arg Phe Gln Ser Thr Lys Glu Phe

420 425

<210> 38

<211> 1281

<212> DNA

<213> 肠道沙门氏菌肠道亚种(Salmonella enterica subsp. Enterica)

<400> 38

gtgatagcaa aattcttccc gtggtatagc gagataactc gtccacaaaa aaatgcttta 60

ttttcagcat ggctgggtta cgtttttgat ggcttcgact ttatgctgat tttctacatt 120

atgtatctga tcaaggctga cttaggattg acagatatgg agggcgcatt ccttgccaca 180

gcggccttta ttgggcgacc atttggcggg gcgctatttg gtctgctggc agataaattt 240

ggccgtaagc cgttaatgat gtggtcgata gttgcctatt ctgtaggtac agggttaagt 300

ggcctggctt ccggtgtaat tatgctgacg cttagtcgtt tcattgtcgg tatggggatg 360

gcgggggagt atgcttgcgc ttctacttat gccgtggaaa gttggccaaa gcatttaaaa 420

tctaaagcga gcgcatttct ggtttcaggt ttcggtattg gtaacatcat agcagcctat 480

tttatgccgt catttgccga agcgtatggt tggcgtgctg ctttttttgt cggtttgcta 540

cccgttcttt tagtaatcta catccgggcc agggctcctg aatctaaaga gtgggaagaa 600

gccaaactca gtggtctcgg aaagcactca caaagtgcct ggtcagtttt ctctttgtca 660

atgaaagggc tatttaatcg agctcaattt ccactgacat tatgtgtatt tattgttctg 720

ttctctattt tcggcgcaaa ctggccgatc tttggtctac tgcctacata tttggcggga 780

gagggctttg atacgggcgt ggtctctaat ttaatgacgg cggcggcatt cggcactgta 840

ttgggaaata tcgtttgggg tctgtgcgca gatagaattg gtttgaagaa aacgttcagc 900

attggtcttc tcatgtcctt tttattcatt ttcccgttat tcagaattcc gcaagataat 960

tatttactgc tgggcgcatg tttattcggt ttaatggcga ctaacgtagg tgttggcggg 1020

ctggttccca aatttctcta cgactacttt cctcttgagg ttcgtggttt gggtaccggg 1080

ctgatttaca atcttgctgc gacatcaggc acattcaatt caatggcggc gacctggctt 1140

ggaataacaa tgggactagg cgttgcgcta acgttcattg ttgctttctg gaccgcaaca 1200

attctactca ttattggctt atccattccg gataggctaa aagcacgtcg tgaaaggttt 1260

cagtcaacaa aagaatttta a 1281

<210> 39

<211> 496

<212> PRT

<213> 弗氏柠檬酸杆菌(Citrobacter freundii)

<400> 39

Met Ser Thr Ser Thr Gln Ser Ile Pro Trp Tyr Arg His Leu Asn Arg

1 5 10 15

Ala Gln Trp Arg Ala Phe Ser Ala Ala Trp Leu Gly Tyr Leu Leu Asp

20 25 30

Gly Phe Asp Phe Val Leu Ile Ala Leu Val Leu Thr Glu Val Gln Gly

35 40 45

Glu Phe Gly Leu Thr Thr Val Gln Ala Ala Ser Leu Ile Ser Ala Ala

50 55 60

Phe Ile Ser Arg Trp Phe Gly Gly Leu Met Leu Gly Ala Met Gly Asp

65 70 75 80

Arg Tyr Gly Arg Arg Leu Ala Met Val Thr Ser Ile Ile Leu Phe Ser

85 90 95

Val Gly Thr Leu Ala Cys Gly Phe Ala Pro Gly Tyr Thr Thr Met Phe

100 105 110

Ile Ala Arg Leu Val Ile Gly Met Gly Met Ala Gly Glu Tyr Gly Ser

115 120 125

Ser Ala Thr Tyr Val Ile Glu Ser Trp Pro Lys His Leu Arg Asn Lys

130 135 140

Ala Ser Gly Phe Leu Ile Ser Gly Phe Ser Val Gly Ala Val Ile Ala

145 150 155 160

Ala Gln Val Tyr Ser Leu Val Val Pro Val Trp Gly Trp Arg Ala Leu

165 170 175

Phe Phe Ile Gly Ile Leu Pro Ile Ile Phe Ala Leu Trp Leu Arg Lys

180 185 190

Asn Ile Pro Glu Ala Glu Asp Trp Lys Glu Lys His Glu Gly Lys Ala

195 200 205

Pro Val Arg Thr Met Val Asp Ile Leu Tyr Arg Gly Glu His Arg Val

210 215 220

Ile Asn Ile Leu Met Thr Leu Ala Ala Ala Thr Ala Leu Trp Phe Cys

225 230 235 240

Phe Ala Gly Asp Leu Gln Asn Ala Ala Ile Val Ala Val Leu Gly Leu

245 250 255

Ile Cys Ala Phe Ile Phe Ile Ser Phe Met Val Gln Ser Ser Gly Lys

260 265 270

Arg Trp Pro Thr Gly Val Met Leu Met Val Val Val Leu Phe Ala Phe

275 280 285

Leu Tyr Ser Trp Pro Ile Gln Ala Leu Leu Pro Thr Tyr Leu Lys Thr

290 295 300

Glu Leu Leu Tyr Asp Pro Ser Thr Val Ala Arg Val Leu Phe Phe Ser

305 310 315 320

Gly Phe Gly Ala Ala Val Gly Cys Cys Val Gly Gly Phe Leu Gly Asp

325 330 335

Trp Leu Gly Thr Arg Lys Ala Tyr Val Cys Ser Leu Leu Ala Ser Gln

340 345 350

Leu Leu Ile Ile Pro Val Phe Ala Ile Gly Gly Ser Asn Val Trp Val

355 360 365

Leu Gly Leu Leu Leu Phe Phe Gln Gln Met Leu Gly Gln Gly Ile Ser

370 375 380

Gly Ile Leu Pro Lys Leu Ile Gly Gly Tyr Phe Asp Thr Asp Gln Arg

385 390 395 400

Ala Ala Gly Leu Gly Phe Thr Tyr Asn Val Gly Ala Leu Gly Gly Ala

405 410 415

Leu Ala Pro Ile Ile Gly Ala Leu Ile Ala Gln Arg Leu Asp Leu Gly

420 425 430

Thr Ala Leu Gly Ser Leu Ser Phe Gly Leu Thr Phe Val Val Ile Leu

435 440 445

Leu Ile Gly Leu Asp Met Pro Ser Arg Val Gln Arg Trp Leu Arg Pro

450 455 460

Glu Ala Leu Arg Thr His Asp Ala Ile Asp Gly Arg Pro Phe Ser Gly

465 470 475 480

Ala Val Pro Phe Gly Gly Asp Lys Ser Thr Met Val Lys Ser Lys Ser

485 490 495

<210> 40

<211> 1491

<212> DNA

<213> 弗氏柠檬酸杆菌(Citrobacter freundii)

<400> 40

atgagtactt ctacccagag catcccgtgg tatcgccatc tcaatcgggc acaatggcgg 60

gcattttccg ccgcctggct gggatatttg cttgatggtt tcgattttgt gttaatcgcc 120

ctggtgctga cagaagtgca gggcgaattc ggattgacga cggtgcaggc cgccagcctg 180

atctcggccg cctttatctc ccgttggttt ggcgggttaa tgctgggggc aatgggcgac 240

cgctacggac gccgcctggc aatggtcacc agcatcattc tgttctcggt gggaacgctg 300

gcctgcggtt tcgcgccggg ctacaccacc atgtttatcg cccgcctggt tatcggtatg 360

ggaatggcgg gtgagtacgg ctccagtgca acctatgtta ttgaaagttg gcctaaacac 420

ctgcgaaaca aagccagtgg tttcctgatt tcaggctttt ccgttggtgc cgtgatcgcc 480

gcccaggtct acagcctggt ggttccggtc tgggggtggc gtgcgctgtt cttcatcggc 540

atcctgccaa ttatcttcgc cctctggctg cgtaaaaata ttccggaagc ggaagactgg 600

aaagagaaac acgaaggcaa agcgccagtg cgcacgatgg tggacattct ctaccggggc 660

gagcatcggg taattaatat tctgatgacg ctcgctgctg ccaccgcgct gtggttctgc 720

tttgcgggcg acctgcaaaa tgcggctatt gtggcggtcc tggggctgat atgcgcgttt 780

atcttcatta gctttatggt gcaaagcagc gggaaacgct ggccgacggg cgtcatgctg 840

atggtggttg tgctgtttgc cttcctgtac tcgtggccga ttcaggcgct gttgccaacc 900

tatctgaaaa ccgaactgct gtacgatcct tccacggtgg ccagggtgct cttctttagc 960

ggctttggtg cggcggtcgg ttgttgcgtc ggcggttttc ttggcgactg gctgggcacg 1020

cgtaaagcct atgtctgcag tctgctggcc tcgcagttgc tgattatacc ggtatttgcg 1080

ataggcggtt caaacgtctg ggtactgggt ttactgctgt ttttccagca aatgctcggc 1140

caggggatct ccgggatctt accaaaactg attggcggct atttcgatac cgatcagcgt 1200

gcggcgggac tgggcttcac ctataacgtg ggcgcactgg gcggggcgct ggcaccgatc 1260

atcggtgcgc tgattgccca acgtctggat ctgggtaccg cgctgggatc cctctctttc 1320

ggtctgacat ttgtagtgat cctgctgatt ggtcttgata tgccgtcccg tgtacagcgc 1380

tggctgcgtc ctgaagcgtt gcgtacgcat gatgccattg atggtcgacc gtttagcggt 1440

gccgtgccgt tcggtggtga caaaagcact atggtgaaat ccaaaagtta a 1491

<210> 41

<211> 451

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 41

Met Asn Thr Ile Phe Lys Gln Lys Asn Thr His Pro Phe Ser Asn Ala

1 5 10 15

Ala Asn Arg Leu Asp Arg Leu Pro Ile Ser Arg Val His Phe Gln Val

20 25 30

Leu Thr Ala Leu Gly Ile Val Tyr Phe Phe Asp Leu Ala Asp Leu Phe

35 40 45

Thr Leu Ser Asn Val Ala Pro Ala Leu Ile Glu His Trp Gly Ile Pro

50 55 60

Leu Ser Thr Ile Ala Asn Val Thr Ala Ala Ser Phe Leu Gly Met Phe

65 70 75 80

Leu Gly Ala Ser Leu Gly Gly Arg Leu Ser Asp Arg Ile Gly Arg Lys

85 90 95

Lys Ala Leu Asn Leu Phe Val Phe Val Phe Ser Ile Ala Ser Leu Cys

100 105 110

Asn Ala Ala Ala Trp Asp Ile Pro Ser Leu Met Thr Phe Arg Phe Leu

115 120 125

Thr Gly Phe Gly Val Ala Ala Ala Met Val Ile Thr Asn Ser Tyr Leu

130 135 140

Ala Glu Phe Phe Pro Ser Ser Val Arg Gly Lys Tyr Ile Ser Phe Cys

145 150 155 160

Ala Met Ile Gly Leu Ile Gly Val Pro Ile Thr Asn Ile Val Ser Ala

165 170 175

Phe Val Ile Pro Leu Gly Ser Trp Gly Trp Arg Leu Val Phe Val Trp

180 185 190

Gly Ala Val Gly Leu Ile Tyr Phe Phe Phe Ile His Arg Leu Glu Glu

195 200 205

Ser Pro Arg Trp His Glu Asn Arg Gly Glu Tyr Ala Lys Ala Asp Ala

210 215 220

Ile Leu Thr Arg Ile Glu Glu Gln Val Glu Lys Glu Lys Gly Pro Leu

225 230 235 240

Pro Ala Ala Ser Gln Pro Lys Val Ser Glu Thr Val Lys Gln Asn Ala

245 250 255

Gly Tyr Ala Gly Leu Leu Lys Gly Arg Asn Leu Lys Ile Thr Ile Val

260 265 270

Leu Ser Ala Val Trp Ile Phe Glu Thr Phe Gly Phe Tyr Gly Phe Ala

275 280 285

Ser Trp Val Pro Ser Leu Leu Lys Ser Asn Gly Val Thr Met Glu Asn

290 295 300

Thr Leu Trp Tyr Asn Val Leu His Ser Val Gly Ala Pro Leu Gly Ala

305 310 315 320

Leu Leu Gly Ser Met Ile Ser Glu Arg Phe Gln Arg Lys Trp Ile Leu

325 330 335

Ala Ala Ser Ala Phe Leu Thr Ala Ile Ala Gly Leu Leu Tyr Gly Met

340 345 350

Thr Phe Ile Pro Ile Met Ile Ile Val Phe Gly Phe Ile Val Asn Ile

355 360 365

Thr Glu Arg Val Phe Thr Ser Asn Leu Tyr Ala Tyr Thr Ser Glu Pro

370 375 380

Tyr Pro Thr Glu Tyr Arg Ser Ser Gly Ser Gly Leu Ala Tyr Gly Leu

385 390 395 400

Gly Arg Phe Ser Asn Ile Phe Gly Ser Leu Leu Val Gly Phe Ile Ala

405 410 415

Val Gln Leu Gly Tyr Ile Ser Val Phe Leu Phe Ile Gly Gly Cys Trp

420 425 430

Leu Ala Cys Ser Leu Leu Leu Ile Phe Phe Gly Pro Asn Thr Asn Ala

435 440 445

Lys Gln Ile

450

<210> 42

<211> 1356

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 42

atgaatacga tcttcaaaca aaagaataca catcctttct caaacgcagc gaatcgttta 60

gaccgccttc cgatttcacg cgttcatttc caagtgttaa ccgctctcgg cattgtttat 120

ttctttgatc tcgcagattt atttaccctc agcaacgtag cgccggcact gatcgagcat 180

tggggcatcc cgctttcaac tattgctaac gtaacggccg cttcgttttt aggcatgttt 240

ttaggcgctt cactgggcgg acggctgtcc gatcgaatcg gccgcaaaaa agccttaaat 300

ctatttgtct ttgttttctc aatcgcatcg ctatgcaatg ctgcggcatg ggacattcca 360

tctttaatga cattccgttt cctcaccgga tttggcgttg cagccgccat ggtcattacg 420

aatagctatt tggcggaatt tttcccttca agtgtacgtg gaaaatatat ttctttttgt 480

gccatgattg gattgattgg ggttccgatc accaacattg tgtctgcctt tgtcattcct 540

cttggttcat ggggatggag gctagtattt gtatggggtg ccgtcggcct tatttatttt 600

ttcttcatcc accgtctgga agagtcacct cgctggcacg aaaatcgcgg ggaatatgcg 660

aaagccgatg cgatcctcac ccgaattgaa gaacaggttg aaaaggagaa aggcccgctt 720

ccggcagcat cccagcctaa agtaagcgaa actgttaagc agaatgcagg ttacgcaggc 780

ttattgaaag gcagaaacct caaaattacc atcgtattat ctgctgtatg gatttttgaa 840

acgtttgggt tttacggatt tgcttcatgg gttccaagcc tgctaaaaag caatggcgta 900

accatggaaa atacattatg gtataacgta ttgcattccg tcggcgctcc acttggcgca 960

ctgctcggct ccatgatttc cgaaagattt caaagaaaat ggattttagc tgcgagtgcg 1020

tttctgacgg ccatcgccgg gctcttatat ggtatgactt ttattcccat catgatcatt 1080

gtatttggtt ttatcgtgaa tatcacagaa cgggtcttta cctcgaactt atacgcctat 1140

acatctgaac cttatccgac tgaataccgc tcgtctggca gcggtttagc ctatggtctt 1200

ggccgttttt caaacatttt tggctcattg cttgtcggat ttattgccgt tcagctcggc 1260

tatatcagcg tcttcttatt tattgggggc tgttggctcg catgctcctt gctgttaatc 1320

ttcttcggtc ctaatacgaa tgcaaaacag atttaa 1356

<210> 43

<211> 543

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<220>

<221> misc_feature

<222> (38)..(38)

<223> Xaa可为任何天然存在的氨基酸

<220>

<221> misc_feature

<222> (197)..(197)

<223> Xaa可为任何天然存在的氨基酸

<220>

<221> misc_feature

<222> (209)..(209)

<223> Xaa可为任何天然存在的氨基酸

<220>

<221> misc_feature

<222> (235)..(235)

<223> Xaa可为任何天然存在的氨基酸

<400> 43

Met Ala Ala Glu Ser Ile Val Ser Arg Asp Glu Ser Ile Ala Ser Leu

1 5 10 15

Glu Lys Ala Glu Gly Arg Ile Thr Tyr Leu Lys Pro Gln Ser Arg Ile

20 25 30

Thr Trp Ser Asp Ala Xaa Lys Tyr Leu Ala Thr Arg Ile Pro Thr Leu

35 40 45

Phe Pro Thr Lys Ala Ser Ile Arg Glu Ala Arg Lys Glu Tyr Pro Ile

50 55 60

Asn Pro Phe Pro Ala Leu Arg Ser Met Asn Trp Leu Gln Thr Gln Tyr

65 70 75 80

Phe Ile Val Gly Phe Leu Ala Trp Thr Trp Asp Ala Leu Asp Phe Phe

85 90 95

Ala Val Ser Leu Asn Met Thr Asn Leu Ala Lys Asp Leu Asp Arg Pro

100 105 110

Val Lys Asp Ile Ser His Ala Ile Thr Leu Val Leu Leu Leu Arg Val

115 120 125

Ile Gly Ala Leu Ile Phe Gly Tyr Leu Gly Asp Arg Tyr Gly Arg Lys

130 135 140

Tyr Ser Phe Val Leu Thr Met Ala Leu Ile Ile Val Ile Gln Ile Gly

145 150 155 160

Thr Gly Phe Val Asn Ser Phe Ser Ala Phe Leu Gly Cys Arg Ala Ile

165 170 175

Phe Gly Ile Ile Met Gly Ser Val Phe Gly Ser Ala Phe Leu Gly Cys

180 185 190

Arg Ala Ile Phe Xaa Ile Ile Met Gly Ser Val Phe Gly Val Ala Ser

195 200 205

Xaa Thr Ala Leu Glu Asn Ala Pro Asn Lys Ala Lys Ser Ile Leu Ser

210 215 220

Gly Ile Phe Gln Glu Gly Tyr Ala Phe Gly Xaa Leu Leu Gly Val Val

225 230 235 240

Phe Gln Arg Ala Ile Val Asp Asn Ser Pro His Gly Trp Arg Ala Ile

245 250 255

Phe Trp Phe Ser Ala Gly Pro Pro Val Leu Phe Ile Ala Trp Arg Leu

260 265 270

Met Leu Pro Glu Ser Gln His Tyr Val Glu Arg Val Arg Leu Glu Lys

275 280 285

Leu Glu Asn Asp Gly Lys Ser Gln Phe Trp Lys Asn Ala Lys Leu Ala

290 295 300

Cys Ser Gln Tyr Trp Leu Ser Met Ile Tyr Leu Val Leu Leu Met Ala

305 310 315 320

Gly Phe Asn Phe Ser Ser His Gly Ser Gln Asp Leu Phe Pro Thr Met

325 330 335

Leu Thr Ser Gln Tyr Gln Phe Ser Ala Asp Ala Ser Thr Val Thr Asn

340 345 350

Ser Val Ala Asn Leu Gly Ala Ile Ala Gly Gly Ile Ile Val Ala His

355 360 365

Ala Ser Ser Phe Phe Gly Arg Arg Phe Ser Ile Ile Val Cys Cys Ile

370 375 380

Gly Gly Gly Ala Met Leu Tyr Pro Trp Gly Phe Val Ala Asn Lys Ser

385 390 395 400

Gly Ile Asn Ala Ser Val Phe Phe Leu Gln Phe Phe Val Gln Gly Ala

405 410 415

Trp Gly Ile Val Pro Ile His Leu Thr Glu Leu Ala Pro Thr Glu Phe

420 425 430

Arg Ala Leu Ile Thr Gly Val Ala Tyr Gln Leu Gly Asn Met Ile Ser

435 440 445

Ser Ala Ser Ser Thr Ile Glu Ala Ser Ile Gly Glu Arg Phe Pro Leu

450 455 460

Glu Gly Arg Glu Asp Ala Tyr Asp Tyr Gly Lys Val Met Cys Ile Phe

465 470 475 480

Met Gly Cys Val Phe Ala Tyr Leu Leu Ile Val Thr Val Leu Gly Pro

485 490 495

Glu Asn Lys Gly Gly Glu Leu Arg Leu Ser Thr Thr Gly Thr Glu Gln

500 505 510

Asp Asp Glu Glu Ser Gln Asn Asn Asn Ile Ile Arg Arg Asn Cys Arg

515 520 525

Gly Trp Thr Ser Phe Gly Ser Lys Phe Gln Ala Arg Asn Ser Thr

530 535 540

<210> 44

<211> 1632

<212> DNA

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 44

atggctgcag aatcaatagt gtctcgcgat gaatccatcg cttcacttga aaaagcagaa 60

ggtagaatca catatttgaa accgcaatct aggatcacat ggagtgatgc taasaaatat 120

ttggctacaa gaatacctac tttgttccca acaaaagcat cgattagaga agcaaggaaa 180

gaatacccta taaatccttt ccctgcctta cgttcgatga actggttgca aacacaatac 240

tttatcgttg ggttcttagc atggacttgg gatgcgttag atttctttgc cgtttcattg 300

aacatgacaa atttggccaa ggatctagac agacctgtaa aagatatttc tcatgccatt 360

actttggtgt tgctattaag ggtcatcggt gctcttatct ttggttattt gggtgacaga 420

tatggtagaa aatactcatt tgttttaact atggctctca ttatcgttat tcaaatcggt 480

acagggttcg ttaattcttt ctctgctttc ttggggtgta gagctatctt tggtatcatt 540

atgggatctg tatttggttc tgctttcttg gggtgtagag ctatctttgk tatcattatg 600

ggatctgtat ttggtgttgc ttctkccact gccttggaaa atgctccaaa caaggctaag 660

tccatccttt ctggtatatt ccaagaaggt tatgctttcg gtwatttatt aggtgtcgtg 720

ttccaaagag ctattgttga taattctcca catggttgga gagctatatt ctggttcagt 780

gccgggcccc cagtgctttt cattgcttgg aggttgatgt tacctgaatc ccaacactat 840

gtcgaaagag tccgtttgga aaaattagaa aacgatggga agtctcaatt ctggaagaat 900

gctaagcttg cctgttctca atattggcta agtatgattt acttggttct tttaatggca 960

ggtttcaact tctcctccca tggttctcaa gatcttttcc caacaatgtt gacttctcaa 1020

taccaattct ccgctgatgc atcaactgtw acaaactctg ttgcaaacct tggtgccatc 1080

gctggtggta tcattgttgc ccatgcctcc tctttctttg gtcgtagatt ctctatcatt 1140

gtatgttgta ttggcggtgg tgctatgtta tacccatggg gttttgttgc taataaatct 1200

ggaattaatg cttcagtctt cttcttacaa ttcttcgtcc aaggtgcttg gggtattgtc 1260

ccaattcatt tgacggaatt agccccaacg gagttcagag ctttgatcac tggtgttgct 1320

taccaattgg gtaatatgat atctagtgcc tcctcaacta tcgaagcctc cattggtgaa 1380

agattcccac ttgaaggtag agaggacgct tatgattatg gtaaggtgat gtgtatcttc 1440

atgggatgcg tgttcgctta cttgttgatc gtaaccgttt tgggcccaga gaacaagggc 1500

ggtgagttga gattatccac tacgggtaca gaacaagacg atgaagaatc tcaaaataac 1560

aatatcattc gaagaaattg tcgcggctgg accagtttcg gatctaaatt tcaagcaaga 1620

aattcaacat aa 1632

<210> 45

<211> 400

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 45

Met Gly Lys Gln Gln Pro Ile Ser Gln Arg Lys Leu Leu Gly Val Ala

1 5 10 15

Gly Leu Gly Trp Leu Phe Asp Ala Met Asp Val Gly Ile Leu Ser Phe

20 25 30

Ile Ile Ala Ala Leu His Val Glu Trp Asn Leu Ser Pro Glu Glu Met

35 40 45

Lys Trp Ile Gly Ser Val Asn Ser Ile Gly Met Ala Ala Gly Ala Phe

50 55 60

Leu Phe Gly Leu Leu Ala Asp Arg Ile Gly Arg Lys Lys Val Phe Ile

65 70 75 80

Ile Thr Leu Leu Cys Phe Ser Ile Gly Ser Gly Ile Ser Ala Phe Val

85 90 95

Thr Ser Leu Ser Ala Phe Leu Ile Leu Arg Phe Val Ile Gly Met Gly

100 105 110

Leu Gly Gly Glu Leu Pro Val Ala Ser Thr Leu Val Ser Glu Ala Val

115 120 125

Val Pro Glu Lys Arg Gly Arg Val Ile Val Leu Leu Glu Ser Phe Trp

130 135 140

Ala Val Gly Trp Leu Ala Ala Ala Leu Ile Ser Tyr Phe Val Ile Pro

145 150 155 160

Ser Phe Gly Trp Gln Ala Ala Leu Leu Leu Thr Ala Leu Thr Ala Phe

165 170 175

Tyr Ala Leu Tyr Leu Arg Thr Ser Leu Pro Asp Ser Pro Lys Tyr Glu

180 185 190

Ser Leu Ser Ala Lys Lys Arg Ser Met Trp Glu Asn Val Lys Ser Val

195 200 205

Trp Ala Arg Gln Tyr Ile Arg Pro Thr Val Met Leu Ser Ile Val Trp

210 215 220

Phe Cys Val Val Phe Ser Tyr Tyr Gly Met Phe Leu Trp Leu Pro Ser

225 230 235 240

Val Met Leu Leu Lys Gly Phe Ser Met Ile Gln Ser Phe Glu Tyr Val

245 250 255

Leu Leu Met Thr Leu Ala Gln Leu Pro Gly Tyr Phe Ser Ala Ala Trp

260 265 270

Leu Ile Glu Lys Ala Gly Arg Lys Trp Ile Leu Val Val Tyr Leu Ile

275 280 285

Gly Thr Ala Gly Ser Ala Tyr Phe Phe Gly Thr Ala Asp Ser Leu Ser

290 295 300

Leu Leu Leu Thr Ala Gly Val Leu Leu Ser Phe Phe Asn Leu Gly Ala

305 310 315 320

Trp Gly Val Leu Tyr Ala Tyr Thr Pro Glu Gln Tyr Pro Thr Ala Ile

325 330 335

Arg Ala Thr Gly Ser Gly Thr Thr Ala Ala Phe Gly Arg Ile Gly Gly

340 345 350

Ile Phe Gly Pro Leu Leu Val Gly Thr Leu Ala Ala Arg His Ile Ser

355 360 365

Phe Ser Val Ile Phe Ser Ile Phe Cys Ile Ala Ile Leu Leu Ala Val

370 375 380

Ala Cys Ile Leu Ile Met Gly Lys Glu Thr Lys Gln Thr Glu Leu Glu

385 390 395 400

<210> 46

<211> 1203

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 46

atgggaaaac aacagcctat atcccagcgt aaactgctgg gcgtcgccgg tttggggtgg 60

ctgtttgatg caatggatgt cggaatatta tcgtttatta tcgccgcgct ccatgtagag 120

tggaatctgt cgcccgaaga aatgaaatgg atcggaagcg tcaattccat cggcatggct 180

gcgggtgcgt ttttgtttgg tttgcttgct gatcgaatcg gccgcaaaaa agtgttcatc 240

atcacccttt tatgcttttc catcggaagc ggcatttccg cttttgtgac gagcttatcg 300

gcatttctaa tccttcgttt cgtgattggt atgggacttg gcggcgagct tccagtcgct 360

tcaacactcg tttcggaagc ggttgtgcct gaaaagcggg gcagagtgat tgtgcttttg 420

gaaagctttt gggccgtggg ctggctcgca gcggccttga tttcttactt tgtgatacca 480

agcttcggct ggcaggctgc ccttctgtta actgcgctga ctgcttttta tgcgctgtac 540

ctgcggacga gtctacctga ttcgccgaaa tatgaatcgc tttctgccaa aaagaggtcg 600

atgtgggaga atgtaaaaag cgtctgggca agacagtata tacggccgac ggtgatgctg 660

tcgatcgttt ggttctgtgt ggtgttttct tattacggca tgttcctatg gctcccgagt 720

gtcatgctgc tgaaaggctt cagcatgatt caaagctttg aatatgtcct gctgatgacg 780

cttgctcagc tacctggcta tttctctgcc gcatggctga ttgaaaaagc gggccggaag 840

tggatactcg tcgtttactt gattggcaca gcaggaagcg cctatttctt cggaacggcg 900

gattccttaa gtcttctgct tacggctgga gtgctgttat cgtttttcaa tctcggtgcg 960

tggggcgtgc tgtatgctta taccccggag caatacccga ctgcgattcg agcaacaggt 1020

tcaggaacga cagcagcgtt tggaagaatc ggcggcatct tcgggccttt gctcgtcgga 1080

accctggcag cccgtcatat ttcgttttcg gtcatctttt caatcttttg cattgcaatc 1140

ttacttgcgg ttgcttgtat tttgattatg gggaaagaaa cgaaacaaac tgagctagaa 1200

tag 1203

<210> 47

<211> 283

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 47

Met Ser Asp Lys Glu Gln Thr Ser Gly Asn Thr Asp Leu Glu Asn Ala

1 5 10 15

Pro Ala Gly Tyr Tyr Ser Ser His Asp Asn Asp Val Asn Gly Val Ala

20 25 30

Glu Asp Glu Arg Pro Ser His Asp Ser Leu Gly Lys Ile Tyr Thr Gly

35 40 45

Gly Asp Asn Asn Glu Tyr Ile Tyr Ile Gly Arg Gln Lys Phe Leu Lys

50 55 60

Ser Asp Leu Tyr Gln Ala Phe Gly Gly Thr Leu Asn Pro Gly Leu Ala

65 70 75 80

Pro Ala Pro Val His Lys Phe Ala Asn Pro Ala Pro Leu Gly Leu Ser

85 90 95

Ala Phe Ala Leu Thr Thr Phe Val Leu Ser Met Phe Asn Ala Arg Ala

100 105 110

Gln Gly Ile Thr Val Pro Asn Val Val Val Gly Cys Ala Met Phe Tyr

115 120 125

Gly Gly Leu Val Gln Leu Ile Ala Gly Ile Trp Glu Ile Ala Leu Glu

130 135 140

Asn Thr Phe Gly Gly Thr Ala Leu Cys Ser Tyr Gly Gly Phe Trp Leu

145 150 155 160

Ser Phe Ala Ala Ile Tyr Ile Pro Trp Phe Gly Ile Leu Glu Ala Tyr

165 170 175

Glu Asp Asn Glu Ser Asp Leu Asn Asn Ala Leu Gly Phe Tyr Leu Leu

180 185 190

Gly Trp Ala Ile Phe Thr Phe Gly Leu Thr Val Cys Thr Met Lys Ser

195 200 205

Thr Val Met Phe Phe Leu Leu Phe Phe Leu Leu Ala Leu Thr Phe Leu

210 215 220

Leu Leu Ser Ile Gly His Phe Ala Asn Arg Leu Gly Val Thr Arg Ala

225 230 235 240

Gly Gly Val Leu Gly Val Val Val Ala Phe Ile Ala Trp Tyr Asn Ala

245 250 255

Tyr Ala Gly Val Ala Thr Lys Gln Asn Ser Tyr Val Leu Ala Arg Pro

260 265 270

Phe Pro Leu Pro Ser Thr Glu Arg Val Ile Phe

275 280

<210> 48

<211> 852

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 48

atgtctgaca aggaacaaac gagcggaaac acagatttgg agaatgcacc agcaggatac 60

tatagttccc atgataacga cgttaatggc gttgcagaag atgaacgtcc atctcatgat 120

tcgttgggca agatttacac tggaggtgat aacaatgaat atatctatat tgggcgtcaa 180

aagtttttga agagcgactt ataccaagcc tttggtggta ccttgaatcc agggttagct 240

cctgctccag tgcacaaatt tgctaatcct gcgcccttag gtctttcagc cttcgcgttg 300

acgacatttg tgctgtccat gttcaatgcg agagcgcaag ggatcactgt tcctaatgtt 360

gtcgtcggtt gtgctatgtt ttatggtggt ttggtgcaat tgattgctgg tatttgggag 420

atagctttgg aaaatacttt tggtggtacc gcattatgtt cttacggtgg gttttggttg 480

agtttcgctg caatttacat tccttggttt ggtatcttgg aagcttacga agacaatgaa 540

tctgatttga ataatgcttt aggattttat ttgttggggt gggccatctt tacgtttggt 600

ttaaccgttt gtaccatgaa atccactgtt atgttctttt tgttgttctt cttactagca 660

ttaactttcc tactgttgtc tattggtcac tttgctaata gacttggtgt cacaagagct 720

ggtggtgtcc tgggagttgt tgttgctttc attgcttggt acaacgcata tgcaggtgtt 780

gctacaaagc agaattcata tgtactggct cgtccattcc cattaccatc tactgaaagg 840

gtaatctttt aa 852

<210> 49

<211> 428

<212> PRT

<213> 皮氏罗尔斯顿菌(Ralstonia pickettii)

<400> 49

Met Trp Leu Lys Glu Thr Thr Gln Gly Glu Arg Lys Thr Leu Phe Ala

1 5 10 15

Ala Phe Val Gly Tyr Gly Val Asp Ala Phe Asp Tyr Met Ile Tyr Thr

20 25 30

Phe Met Ile Pro Thr Phe Ile Leu Val Trp Gly Met Thr Lys Ala Glu

35 40 45

Ala Gly Tyr Ile Ala Thr Gly Ala Leu Ile Ser Ser Ala Val Gly Gly

50 55 60

Trp Leu Ala Gly Ile Leu Ala Asp Lys Tyr Gly Arg Val Arg Ile Leu

65 70 75 80

Gln Leu Thr Val Leu Trp Phe Ser Phe Phe Thr Phe Leu Ser Gly Phe

85 90 95

Thr Gln Ser Pro Glu Gln Leu Phe Val Thr Arg Met Leu Gln Gly Leu

100 105 110

Gly Phe Gly Gly Glu Trp Ser Val Gly Ser Val Leu Ile Ala Glu Met

115 120 125

Ile Arg Ala Arg His Arg Gly Lys Ala Val Gly Leu Val Gln Ser Ser

130 135 140

Trp Ala Val Gly Trp Gly Leu Ser Ala Ile Ala Phe Trp Ala Val Tyr

145 150 155 160

Ala Ala Phe Glu Gln Gln Tyr Ala Trp Arg Val Leu Phe Trp Ile Gly

165 170 175

Val Leu Pro Ala Leu Phe Ile Leu Tyr Ile Arg Arg Asn Ile Ser Glu

180 185 190

Pro Glu Val Tyr Gln Glu Thr Lys Ala Lys Leu Ala Arg Thr Gly Gln

195 200 205

Ser Asn Asn Phe Met Leu Ile Phe Lys Pro Gly Val Leu Arg Thr Thr

210 215 220

Val Leu Ala Ser Leu Leu Ala Thr Gly Met Gln Gly Ala Tyr Tyr Ser

225 230 235 240

Val Thr Thr Trp Leu Pro Thr Tyr Leu Lys Met Glu Arg Asn Leu Ser

245 250 255

Val Leu Asn Thr Ser Gly Tyr Leu Met Val Leu Ile Ala Gly Ser Phe

260 265 270

Ala Gly Tyr Leu Thr Ser Ala Trp Leu Ser Asp Arg Leu Gly Arg Arg

275 280 285

Arg Cys Phe Met Leu Phe Ala Val Ser Ala Ala Ile Leu Val Ile Cys

290 295 300

Tyr Thr Gln Leu Pro Ile Thr Asp Ala Ala Met Leu Leu Leu Gly Phe

305 310 315 320

Pro Leu Gly Phe Phe Leu Ser Gly Ile Phe Ser Gly Met Gly Ala Tyr

325 330 335

Leu Thr Glu Leu Tyr Pro Ser His Ile Arg Gly Ser Gly Gln Gly Phe

340 345 350

Ser Tyr Asn Phe Gly Arg Ala Val Gly Ser Val Phe Pro Ala Met Ile

355 360 365

Gly His Met Ser Ala Ser Met Ser Leu Gly Val Ala Ile Gly Tyr Leu

370 375 380

Ala Ala Gly Ala Tyr Gly Leu Val Ile Ile Ala Cys Leu Leu Leu Pro

385 390 395 400

Glu Thr Gln Gly Arg Glu Leu Leu Gly Glu Arg Glu Ala Gly Thr Glu

405 410 415

Gly Pro Ala Asp Ala Ala Thr Ser Arg Thr Val Ala

420 425

<210> 50

<211> 1287

<212> DNA

<213> 皮氏罗尔斯顿菌(Ralstonia pickettii)

<400> 50

atgtggctta aagaaacaac gcagggagag cgcaagacgc tctttgccgc cttcgtcggc 60

tacggggtcg atgcattcga ctacatgatc tacaccttca tgatccccac cttcatcctg 120

gtgtggggca tgaccaaggc cgaggccggc tatatcgcga cgggcgcgct catcagctcg 180

gccgtcggcg gctggctggc cggcatcctg gccgacaaat atggccgcgt gcggatcctg 240

cagctgaccg tgctctggtt cagtttcttt accttcctga gcggattcac ccagtctccc 300

gagcaactgt tcgtgacccg catgctgcaa gggctggggt tcggcggcga atggtcggtc 360

ggttcggtgc tgatcgccga gatgatccgt gcccgccacc gcggcaaggc ggttggcctg 420

gtgcaaagca gctgggcagt cggctggggc ctgtcggcga ttgccttctg ggcggtctat 480

gccgcgttcg agcagcaata cgcatggcgc gtgctgttct ggatcggcgt gctgccggcg 540

ctctttatcc tctacatccg ccgcaatatc tccgaacccg aggtgtatca ggaaaccaag 600

gccaagctgg cccgcaccgg gcaaagcaat aacttcatgc tgatcttcaa gcccggcgtg 660

ctgcgcacca cggtgctggc cagcctgctg gcgaccggca tgcaaggtgc ctactattcg 720

gtgacgacct ggctgccgac ctatctcaag atggagcgca acctgtccgt gctcaatacc 780

agcggctacc tgatggtgct gatcgcaggc tccttcgccg gctatctgac cagcgcctgg 840

ctgtccgacc gcctggggcg ccggcgttgc ttcatgctgt tcgcagtgag cgcagccatc 900

ctcgtgatct gctatacgca gctgccgatc accgacgcgg cgatgctgct gcttggcttc 960

cccttgggct tcttcctgtc tggcatcttt tcgggcatgg gagcttacct gacggagctg 1020

tatccgagcc atatccgtgg ctccggacaa ggcttttcct acaacttcgg gcgcgcggtc 1080

ggctcggtgt tcccggccat gatcgggcat atgagcgcat cgatgtcgct gggcgtggcc 1140

atcggctatc tcgccgcggg cgcctatggg ttggtcatta tcgcgtgcct gctgttgccg 1200

gaaacacagg gacgcgaact actcggcgaa cgcgaggctg gcacggaagg accggctgac 1260

gcagcaactt cgcggacggt agcctga 1287

<210> 51

<211> 188

<212> PRT

<213> 肺炎克雷伯氏菌(Klebsiella pneumoniae)

<400> 51

Met Gly Asn Thr Lys Leu Ala Asn Pro Ala Pro Leu Gly Leu Met Gly

1 5 10 15

Phe Gly Met Thr Thr Ile Leu Leu Asn Leu Ala Asn Ser Gly Leu Phe

20 25 30

Ala Phe Asp Val Ala Ile Leu Ala Met Gly Ile Phe Tyr Gly Gly Ile

35 40 45

Ala Gln Ile Phe Ala Gly Leu Leu Glu Tyr Lys Lys Gly Asn Thr Phe

50 55 60

Gly Leu Thr Ala Phe Thr Ser Tyr Gly Ser Phe Trp Leu Thr Leu Val

65 70 75 80

Ala Ile Leu Leu Met Pro Lys Met Gly Leu Ala Asp Ala Pro His Ala

85 90 95

His Phe Leu Gly Met Tyr Leu Gly Leu Trp Gly Val Phe Thr Leu Phe

100 105 110

Met Phe Phe Gly Thr Leu Lys Ala Ala Arg Met Leu Gln Phe Val Phe

115 120 125

Leu Ser Leu Thr Val Leu Phe Ala Leu Leu Ala Ile Gly His Leu Ala

130 135 140

Asp Asn Glu Gly Ile Val Lys Val Ala Gly Trp Val Gly Leu Ile Cys

145 150 155 160

Gly Ala Ser Ala Ile Tyr Leu Ala Met Gly Glu Val Leu Asn Glu Gln

165 170 175

Phe Gly Arg Thr Val Leu Pro Ile Gly Glu Pro Arg

180 185

<210> 52

<211> 567

<212> DNA

<213> 肺炎克雷伯氏菌(Klebsiella pneumoniae)

<400> 52

atgggcaaca ctaagttggc taatccggca ccgctgggcc ttatgggctt cggcatgacc 60

actattctgc ttaacctggc gaatagcggc ctgttcgcat tcgatgtagc tatcctggcg 120

atgggcattt tttacggcgg cattgcgcaa atttttgccg gcctgctgga atacaaaaaa 180

ggcaacactt ttggcttaac cgcttttacc tcctacggca gcttctggct gacgctggtg 240

gcgattcttc tgatgccgaa aatgggcctg gcagacgcgc cacacgcgca cttcctcggt 300

atgtacctcg gcctgtgggg cgtctttact ctgtttatgt tctttggcac cctgaaggca 360

gcccgcatgc tgcagtttgt cttcctgagc ctgaccgtac tgttcgcgct gctggcgatt 420

ggccatttgg ccgataacga aggcattgtg aaggtagccg gctgggtcgg cctgatttgt 480

ggcgccagcg ctatttacct ggcgatgggt gaagtgctga acgaacagtt cggccgcacc 540

gtgctgccga tcggcgaacc gcgctaa 567

<210> 53

<211> 188

<212> PRT

<213> 柠檬酸杆菌属(Citrobacter sp.)

<400> 53

Met Gly Asn Thr Lys Leu Ala Asn Pro Ala Pro Leu Gly Leu Met Gly

1 5 10 15

Phe Gly Met Thr Thr Ile Leu Leu Asn Leu His Asn Ala Gly Phe Phe

20 25 30

Ala Leu Asp Val Ile Ile Leu Ala Met Gly Ile Phe Tyr Gly Gly Ile

35 40 45

Ala Gln Ile Phe Ala Gly Leu Leu Glu Tyr Lys Lys Gly Asn Thr Phe

50 55 60

Gly Leu Thr Ala Phe Thr Ser Tyr Gly Ser Phe Trp Leu Thr Leu Val

65 70 75 80

Ala Ile Leu Leu Met Pro Lys Met Gly Leu Ala Asp Ala Thr Asn Gly

85 90 95

Gln Phe Leu Gly Ala Tyr Leu Gly Leu Trp Gly Val Phe Thr Leu Phe

100 105 110

Met Phe Ile Gly Thr Leu Lys Ala Ala Arg Met Leu Gln Phe Val Phe

115 120 125

Leu Ser Leu Thr Val Leu Phe Ala Leu Leu Ala Val Gly Asn Ile Ala

130 135 140

Gly Asn Glu Ala Ile Ile His Val Ala Gly Trp Val Gly Leu Val Cys

145 150 155 160

Gly Ala Ser Ala Ile Tyr Leu Ala Met Gly Glu Val Leu Asn Glu Gln

165 170 175

Phe Gly Arg Thr Ile Leu Pro Ile Gly Glu Ala His

180 185

<210> 54

<211> 567

<212> DNA

<213> 柠檬酸杆菌属(Citrobacter sp.)

<400> 54

atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt tggcatgacc 60

accattctgc ttaacctgca caacgcgggt ttcttcgctc tggacgttat tattctggcg 120

atgggcattt tctacggcgg tatcgcgcaa atttttgccg gactgctgga atataaaaaa 180

ggcaacacct tcggtttaac cgcctttacc tcctacggtt cgttctggct gacgctggtt 240

gcgatcctgc tgatgccgaa aatgggcctg gcggatgcaa ccaatggtca gttcctgggc 300

gcttacctcg gtctgtgggg cgtgttcact ctgttcatgt tcatcggtac gctgaaagcg 360

gcgcgcatgc tgcagttcgt gttcctgagc ctgacagtgc tgttcgccct gctggccgtg 420

ggcaacattg cgggtaacga agcgatcatt cacgttgctg gctgggtagg cttagtgtgt 480

ggcgcaagcg ccatttacct ggcgatgggt gaagtgctga acgaacaatt tggccgtacc 540

atcctgccga ttggtgaagc gcactaa 567

<210> 55

<211> 380

<212> PRT

<213> 米曲霉(Aspergillus oryzae)

<400> 55

Met Leu Thr Pro Pro Lys Phe Glu Asp Glu Lys Gln Leu Gly Pro Val

1 5 10 15

Gly Ile Arg Glu Arg Leu Arg His Phe Thr Trp Ala Trp Tyr Thr Leu

20 25 30

Thr Met Ser Gly Gly Gly Leu Ala Val Leu Ile Ile Ser Gln Pro Phe

35 40 45

Gly Phe Arg Gly Leu Arg Glu Ile Gly Ile Ala Val Tyr Ile Leu Asn

50 55 60

Leu Ile Leu Phe Ala Leu Val Cys Ser Thr Met Ala Ile Arg Phe Ile

65 70 75 80

Leu His Gly Asn Leu Leu Glu Ser Leu Arg His Asp Arg Glu Gly Leu

85 90 95

Phe Phe Pro Thr Phe Trp Leu Ser Val Ala Thr Ile Ile Cys Gly Leu

100 105 110

Ser Arg Tyr Phe Gly Glu Glu Ser Asn Glu Ser Phe Gln Leu Ala Leu

115 120 125

Glu Ala Leu Phe Trp Ile Tyr Cys Val Cys Thr Leu Leu Val Ala Ile

130 135 140

Ile Gln Tyr Ser Phe Val Phe Ser Ser His Lys Tyr Gly Leu Gln Thr

145 150 155 160

Met Met Pro Ser Trp Ile Leu Pro Ala Phe Pro Ile Met Leu Ser Gly

165 170 175

Thr Ile Ala Ser Val Ile Gly Glu Gln Gln Pro Ala Arg Ala Ala Leu

180 185 190

Pro Ile Ile Gly Ala Gly Val Thr Phe Gln Gly Leu Gly Phe Ser Ile

195 200 205

Ser Phe Met Met Tyr Ala His Tyr Ile Gly Arg Leu Met Glu Ser Gly

210 215 220

Leu Pro His Ser Asp His Arg Pro Gly Met Phe Ile Cys Val Gly Pro

225 230 235 240

Pro Ala Phe Thr Ala Leu Ala Leu Val Gly Met Ser Lys Gly Leu Pro

245 250 255

Glu Asp Phe Lys Leu Leu His Asp Ala His Ala Leu Glu Asp Gly Arg

260 265 270

Ile Ile Glu Leu Leu Ala Ile Ser Ala Gly Val Phe Leu Trp Ala Leu

275 280 285

Ser Leu Trp Phe Phe Cys Ile Ala Ile Val Ala Val Ile Arg Ser Pro

290 295 300

Pro Glu Ala Phe His Leu Asn Trp Trp Ala Met Val Phe Pro Asn Thr

305 310 315 320

Gly Phe Thr Leu Ala Thr Ile Thr Leu Gly Lys Ala Leu Asn Ser Asn

325 330 335

Gly Val Lys Gly Val Gly Ser Ala Met Ser Ile Cys Ile Val Cys Met

340 345 350

Tyr Ile Phe Val Phe Val Asn Asn Val Arg Ala Val Ile Arg Lys Asp

355 360 365

Ile Met Tyr Pro Gly Lys Asp Glu Asp Val Ser Asp

370 375 380

<210> 56

<211> 1143

<212> DNA

<213> 米曲霉(Aspergillus oryzae)

<400> 56

atgctgacac ctcccaagtt tgaggatgag aagcagctgg gccccgtggg tatccgggag 60

aggcttcgcc atttcacttg ggcctggtac acattaacga tgagtggagg agggctggcc 120

gtcctcatca tcagccagcc ctttgggttc cgcggattga gagagatcgg catcgctgtc 180

tatatcctca acctgatcct cttcgccctt gtctgctcta ccatggctat aaggttcatc 240

ctgcacggca accttctgga gtccctccgt catgaccgcg agggtctctt cttcccgacc 300

ttctggctct ccgtcgcaac catcatctgc ggcttgtctc gctacttcgg tgaagaatcg 360

aatgagtcct tccaactagc cctcgaagcc ctcttctgga tctactgcgt ctgcacctta 420

ctcgtcgcaa tcatccaata ctcgttcgtc ttctcatccc acaagtacgg ccttcaaacc 480

atgatgcctt catggatcct tccagccttc cccatcatgc tcagcggcac catcgcctcc 540

gtcatcggtg aacaacaacc cgctcgcgca gccctcccca tcatcggcgc cggcgtcacc 600

ttccagggcc tcggcttctc catcagcttc atgatgtacg cccactacat cggccgactg 660

atggagtccg gcctccccca cagcgaccac agaccaggca tgttcatctg cgtcggaccc 720

cccgccttca cagccctcgc cctcgtcggc atgagcaaag gcctccccga agacttcaag 780

ctgctccacg acgcccacgc cctggaagat ggccgcatca tcgagctgct ggccatctcc 840

gccggcgtct tcctctgggc cctgagtctc tggttcttct gcatcgccat tgtcgccgtc 900

atccgctcgc cccccgaggc cttccacctc aactggtggg ccatggtctt ccccaacacc 960

ggcttcaccc tggccaccat caccctgggc aaggctctca acagtaacgg cgtgaagggc 1020

gtcggctccg ccatgtctat ctgcatcgtg tgcatgtaca tcttcgtctt tgtcaacaat 1080

gtccgcgccg ttatccggaa ggatatcatg tacccgggta aagatgagga tgtatctgat 1140

tag 1143

<210> 57

<211> 438

<212> PRT

<213> 粟酒裂殖酵母(Schizosaccharomyces pombe)

<400> 57

Met Gly Glu Leu Lys Glu Ile Leu Lys Gln Arg Tyr His Glu Leu Leu

1 5 10 15

Asp Trp Asn Val Lys Ala Pro His Val Pro Leu Ser Gln Arg Leu Lys

20 25 30

His Phe Thr Trp Ser Trp Phe Ala Cys Thr Met Ala Thr Gly Gly Val

35 40 45

Gly Leu Ile Ile Gly Ser Phe Pro Phe Arg Phe Tyr Gly Leu Asn Thr

50 55 60

Ile Gly Lys Ile Val Tyr Ile Leu Gln Ile Phe Leu Phe Ser Leu Phe

65 70 75 80

Gly Ser Cys Met Leu Phe Arg Phe Ile Lys Tyr Pro Ser Thr Ile Lys

85 90 95

Asp Ser Trp Asn His His Leu Glu Lys Leu Phe Ile Ala Thr Cys Leu

100 105 110

Leu Ser Ile Ser Thr Phe Ile Asp Met Leu Ala Ile Tyr Ala Tyr Pro

115 120 125

Asp Thr Gly Glu Trp Met Val Trp Val Ile Arg Ile Leu Tyr Tyr Ile

130 135 140

Tyr Val Ala Val Ser Phe Ile Tyr Cys Val Met Ala Phe Phe Thr Ile

145 150 155 160

Phe Asn Asn His Val Tyr Thr Ile Glu Thr Ala Ser Pro Ala Trp Ile

165 170 175

Leu Pro Ile Phe Pro Pro Met Ile Cys Gly Val Ile Ala Gly Ala Val

180 185 190

Asn Ser Thr Gln Pro Ala His Gln Leu Lys Asn Met Val Ile Phe Gly

195 200 205

Ile Leu Phe Gln Gly Leu Gly Phe Trp Val Tyr Leu Leu Leu Phe Ala

210 215 220

Val Asn Val Leu Arg Phe Phe Thr Val Gly Leu Ala Lys Pro Gln Asp

225 230 235 240

Arg Pro Gly Met Phe Met Phe Val Gly Pro Pro Ala Phe Ser Gly Leu

245 250 255

Ala Leu Ile Asn Ile Ala Arg Gly Ala Met Gly Ser Arg Pro Tyr Ile

260 265 270

Phe Val Gly Ala Asn Ser Ser Glu Tyr Leu Gly Phe Val Ser Thr Phe

275 280 285

Met Ala Ile Phe Ile Trp Gly Leu Ala Ala Trp Cys Tyr Cys Leu Ala

290 295 300

Met Val Ser Phe Leu Ala Gly Phe Phe Thr Arg Ala Pro Leu Lys Phe

305 310 315 320

Ala Cys Gly Trp Phe Ala Phe Ile Phe Pro Asn Val Gly Phe Val Asn

325 330 335

Cys Thr Ile Glu Ile Gly Lys Met Ile Asp Ser Lys Ala Phe Gln Met

340 345 350

Phe Gly His Ile Ile Gly Val Ile Leu Cys Ile Gln Trp Ile Leu Leu

355 360 365

Met Tyr Leu Met Val Arg Ala Phe Leu Val Asn Asp Leu Cys Tyr Pro

370 375 380

Gly Lys Asp Glu Asp Ala His Pro Pro Pro Lys Pro Asn Thr Gly Val

385 390 395 400

Leu Asn Pro Thr Phe Pro Pro Glu Lys Ala Pro Ala Ser Leu Glu Lys

405 410 415

Val Asp Thr His Val Thr Ser Thr Gly Gly Glu Ser Asp Pro Pro Ser

420 425 430

Ser Glu His Glu Ser Val

435

<210> 58

<211> 1946

<212> DNA

<213> 粟酒裂殖酵母(Schizosaccharomyces pombe)

<400> 58

ttcattttct ctcttggcca ctattttttt ttttaattcc cctttatctc tcgattcgac 60

atgggtgaac tcaaggaaat cttgaaacag aggtatcatg agttgcttga ctggaatgtc 120

aaagcccctc atgtccctct cagtcaacga ctgaagcatt ttacatggtc ttggtttgca 180

tgtactatgg caactggtgg tgttggtttg attattggtt ctttcccctt tcgattttat 240

ggtcttaata caattggcaa aattgtttat attcttcaaa tctttttgtt ttctctcttt 300

ggatcatgca tgctttttcg ctttattaaa tatccttcaa ctatcaagga ttcctggaac 360

catcatttgg aaaagctttt cattgctact tgtcttcttt caatatccac gttcatcgac 420

atgcttgcca tatacgccta tcctgatacc ggcgagtgga tggtgtgggt cattcgaatc 480

ctttattaca tttacgttgc agtatccttt atatactgcg taatggcttt ttttacaatt 540

ttcaacaacc atgtatatac cattgaaacc gcatctcctg cttggattct tcctattttc 600

cctcctatga tttgtggtgt cattgctggc gccgtcaatt ctacacaacc cgctcatcaa 660

ttaaaaaata tggttatctt tggtatcctc tttcaaggac ttggtttttg ggtttatctt 720

ttactgtttg ccgtcaatgt cttacggttt tttactgtag gcctggcaaa accccaagat 780

cgacctggta tgtttatgtt tgtcggtcca ccagctttct caggtttggc cttaattaat 840

attgcgcgtg gtgctatggg cagtcgccct tatatttttg ttggcgccaa ctcatccgag 900

tatcttggtt ttgtttctac ctttatggct atttttattt ggggtcttgc tgcttggtgt 960

tactgtctcg ccatggttag ctttttagcg ggctttttca ctcgagcccc tctcaagttt 1020

gcttgtggat ggtttgcatt cattttcccc aacgtgggtt ttgttaattg taccattgag 1080

ataggtaaaa tgatagattc caaagctttc caaatgtttg gacatatcat tggggtcatt 1140

ctttgtattc agtggatcct cctaatgtat ttaatggtcc gtgcgtttct cgtcaatgat 1200

ctttgctatc ctggcaaaga cgaagatgcc catcctccac caaaaccaaa tacaggtgtc 1260

cttaacccta ccttcccacc tgaaaaagca cctgcatctt tggaaaaagt cgatacacat 1320

gtcacatcta ctggtggtga atcggatcct cctagtagtg aacatgaaag cgtttaagct 1380

tgtatgcttt tccttaattt ttctataaat ctgtgtgccc tgctcttaat accattatag 1440

attaatcatt ttgaatcatt ctgtatcttt attgtactac tggtactaat tttgcttaga 1500

catttttgct ccttcttctt ctttttgttt aaattataca taccaaaatt ttggactttg 1560

aataatggta atttttggtt gtcgtagtgt taaatatgta tgcgtcttgc atatgaatca 1620

cgacgaagga atcaattaaa aaatcaatcc tgtacataat aaaattaagt ttatttattt 1680

cattttatcg gatttaatcg tctaaaattt atatcttggt catccaagct tatatctctt 1740

tctactctta tcagcagcac actttagtta tggttatttg aaaacttgtg tataaattcc 1800

tggttataga gaaaatgagt ataagacaac aaaaaaaagc ctagtcggca tgcgacatgt 1860

ctcaaacata tctttggcgt attgatgagc atcttacaca ctcactatac gtaacaataa 1920

aattaagagg gatttcatga caaaag 1946

<210> 59

<211> 383

<212> PRT

<213> 假单胞菌属菌种(Pseudomonas sp.)

<400> 59

Met Ile Cys Pro Asn Ser Ala Lys Pro Gly Ile Lys Pro Phe Ser Gln

1 5 10 15

Leu Gln His Pro Arg Glu Val Ile Arg Gln Phe Thr Pro Asn Trp Phe

20 25 30

Ala Ala Thr Met Gly Thr Gly Val Leu Ala Leu Ala Leu Ala Gln Leu

35 40 45

Pro Val Ala Ile Pro Gly Leu His Ala Val Ala Glu Gly Leu Trp Leu

50 55 60

Phe Asn Ile Leu Leu Phe Thr Leu Phe Thr Phe Ala Tyr Ala Ala Arg

65 70 75 80

Trp Ile Leu Phe Phe Asp Glu Ala Arg Arg Ile Phe Gly His Ser Thr

85 90 95

Val Ser Met Phe Phe Gly Thr Ile Pro Met Gly Leu Ala Thr Ile Ile

100 105 110

Asn Gly Phe Leu Val Phe Gly Leu Pro Arg Trp Gly Glu Gly Val Ile

115 120 125

His Leu Ala Glu Val Leu Trp Trp Leu Asp Val Ala Met Ser Leu Ala

130 135 140

Cys Gly Val Leu Ile Pro Tyr Met Met Phe Thr Arg Gln Glu His Ser

145 150 155 160

Ile Asp Gln Met Thr Ala Val Trp Leu Leu Pro Val Val Ala Ala Glu

165 170 175

Val Ala Ala Ala Ser Gly Gly Leu Leu Ala Pro His Leu Ala Asp Ala

180 185 190

His Ala Gln Leu Val Val Leu Thr Thr Ser Tyr Val Leu Trp Ala Phe

195 200 205

Ser Leu Pro Val Ala Phe Ser Ile Leu Thr Ile Leu Leu Leu Arg Met

210 215 220

Ala Leu His Lys Leu Pro His Glu Asn Met Ala Ala Ser Ser Trp Leu

225 230 235 240

Ala Leu Gly Pro Ile Gly Thr Gly Ala Leu Gly Met Leu Leu Leu Gly

245 250 255

Ser Asp Ala Pro Ala Ile Phe Ala Ala Asn Gly Leu Pro Gly Ile Gly

260 265 270

Glu Ile Ala Ala Gly Leu Gly Leu Val Ala Gly Ile Thr Leu Trp Gly

275 280 285

Phe Gly Leu Trp Trp Met Leu Met Ala Leu Leu Ile Thr Ala Arg Tyr

290 295 300

Leu Arg Asp Gly Ile Pro Phe Asn Leu Gly Trp Trp Gly Phe Thr Phe

305 310 315 320

Pro Leu Gly Val Tyr Ser Leu Ala Thr Leu Lys Leu Ala Ser Thr Leu

325 330 335

Asn Leu Gly Phe Phe Ser Val Val Gly Cys Val Leu Val Ser Leu Leu

340 345 350

Ala Val Met Trp Leu Ile Val Gly Lys Arg Thr Val Gln Gly Ala Trp

355 360 365

Arg Gly Glu Leu Phe Val Ser Pro Cys Ile Ala Gly Leu Lys Gln

370 375 380

<210> 60

<211> 1143

<212> DNA

<213> 假单胞菌属菌种(Pseudomonas sp.)

<400> 60

atgctgacac ctcccaagtt tgaggatgag aagcagctgg gccccgtggg tatccgggag 60

aggcttcgcc atttcacttg ggcctggtac acattaacga tgagtggagg agggctggcc 120

gtcctcatca tcagccagcc ctttgggttc cgcggattga gagagatcgg catcgctgtc 180

tatatcctca acctgatcct cttcgccctt gtctgctcta ccatggctat aaggttcatc 240

ctgcacggca accttctgga gtccctccgt catgaccgcg agggtctctt cttcccgacc 300

ttctggctct ccgtcgcaac catcatctgc ggcttgtctc gctacttcgg tgaagaatcg 360

aatgagtcct tccaactagc cctcgaagcc ctcttctgga tctactgcgt ctgcacctta 420

ctcgtcgcaa tcatccaata ctcgttcgtc ttctcatccc acaagtacgg ccttcaaacc 480

atgatgcctt catggatcct tccagccttc cccatcatgc tcagcggcac catcgcctcc 540

gtcatcggtg aacaacaacc cgctcgcgca gccctcccca tcatcggcgc cggcgtcacc 600

ttccagggcc tcggcttctc catcagcttc atgatgtacg cccactacat cggccgactg 660

atggagtccg gcctccccca cagcgaccac agaccaggca tgttcatctg cgtcggaccc 720

cccgccttca cagccctcgc cctcgtcggc atgagcaaag gcctccccga agacttcaag 780

ctgctccacg acgcccacgc cctggaagat ggccgcatca tcgagctgct ggccatctcc 840

gccggcgtct tcctctgggc cctgagtctc tggttcttct gcatcgccat tgtcgccgtc 900

atccgctcgc cccccgaggc cttccacctc aactggtggg ccatggtctt ccccaacacc 960

ggcttcaccc tggccaccat caccctgggc aaggctctca acagtaacgg cgtgaagggc 1020

gtcggctccg ccatgtctat ctgcatcgtg tgcatgtaca tcttcgtctt tgtcaacaat 1080

gtccgcgccg ttatccggaa ggatatcatg tacccgggta aagatgagga tgtatctgat 1140

tag 1143

<210> 61

<211> 386

<212> PRT

<213> 比林欧文氏菌(Erwinia billingiae)

<400> 61

Met Asn Asp Lys Ile Phe Thr Leu His Asn Gly Ala Arg Pro Leu Ser

1 5 10 15

Ala Leu Ser Ser Pro Lys Glu Ala Ile Arg Gln Phe Thr Pro Asn Trp

20 25 30

Phe Ala Ala Thr Met Gly Thr Gly Ile Leu Ser Leu Ser Leu Ala Gln

35 40 45

Phe Pro Trp Gln Val Pro Gly Leu Arg Leu Val Ala Glu Gly Leu Trp

50 55 60

Met Phe Asn Ile Leu Leu Phe Ser Val Phe Ala Val Met Tyr Leu Ala

65 70 75 80

Arg Trp Val Met Tyr Phe Gly Glu Ala Lys Arg Ile Phe Gly His Ser

85 90 95

Thr Val Ser Met Phe Phe Gly Thr Ile Pro Met Gly Met Ala Thr Ile

100 105 110

Ile Asn Gly Leu Leu Ala Phe Gly Leu Pro Arg Trp Gly Ala Asp Val

115 120 125

Ile Gln Val Ala His Ala Leu Trp Trp Leu Asp Val Ala Met Ser Leu

130 135 140

Leu Cys Gly Val Cys Ile Pro Tyr Met Met Phe Thr Arg Gln Gln His

145 150 155 160

Ser Ile Asp Gln Met Thr Ala Val Trp Leu Leu Pro Val Val Ala Ala

165 170 175

Glu Val Ala Ala Val Ser Gly Gly Leu Leu Ala Pro His Ile Ala Ala

180 185 190

Pro His Ala Gln Leu Gly Ile Ile Ile Thr Ser Tyr Val Leu Trp Ala

195 200 205

Tyr Ser Val Pro Val Ala Leu Ser Ile Leu Ala Ile Leu Leu Leu Arg

210 215 220

Met Ala Leu His Lys Leu Pro His Glu Ser Met Ala Ala Ser Ser Trp

225 230 235 240

Leu Ala Leu Gly Pro Ile Gly Thr Gly Ser Leu Gly Met Leu Val Ile

245 250 255

Gly Gly Asp Ala Pro Gly Val Phe Ala Ala Ala Gly Met Pro Glu Ile

260 265 270

Gly His Ile Ala Gln Gly Val Gly Phe Val Ala Gly Ile Leu Phe Trp

275 280 285

Gly Cys Gly Leu Trp Trp Met Leu Leu Ala Thr Leu Ile Thr Val Arg

290 295 300

Tyr Phe Arg Glu Gly Ile Pro Phe Asn Leu Gly Trp Trp Gly Phe Thr

305 310 315 320

Phe Pro Leu Gly Val Tyr Thr Val Ala Thr Leu Lys Leu Ser Thr Leu

325 330 335

Ile Asp Leu Ala Phe Phe Lys Gly Phe Gly Ala Leu Leu Val Val Ile

340 345 350

Leu Ala Ala Met Trp Leu Leu Val Ala Val Lys Thr Val Arg Gly Ala

355 360 365

Trp Ser Gly Lys Leu Phe Val Ser Pro Cys Leu Ala Gly Leu Asp Arg

370 375 380

Lys Pro

385

<210> 62

<211> 1161

<212> DNA

<213> 比林欧文氏菌(Erwinia billingiae)

<400> 62

atgaacgata aaatattcac cctgcataac ggtgcgcgtc cgctgagcgc gctgagcagc 60

ccgaaagagg ccatacgaca gttcacgcct aactggttcg ccgcgaccat ggggaccggc 120

atcctgtcgc tgtctctggc gcagttcccc tggcaggttc cgggattgcg ccttgtcgcc 180

gaggggctct ggatgtttaa catcctcctg ttcagcgtct ttgccgtgat gtatctggcc 240

cgctgggtga tgtacttcgg agaggcaaag cgcattttcg gccattcaac cgtgtccatg 300

ttcttcggca ccatcccgat gggcatggcg accatcatca atggcctgct ggcgttcggt 360

cttccgcgct ggggcgcaga cgttattcag gtggcacacg ccctgtggtg gctggacgtg 420

gcgatgtcgc tgctgtgcgg cgtgtgcatt ccctacatga tgtttacccg gcagcagcac 480

agtattgacc agatgactgc cgtctggtta ctgccggtcg tggcagccga ggtcgcggcg 540

gtcagcggcg gcctgctggc gccgcacatt gctgccccac acgcgcagct cggcatcatt 600

attaccagct acgtgctgtg ggcgtactcc gtgccggtgg cgctgagcat cctcgccatt 660

ctgctgctgc gtatggcgct gcacaagcta ccgcacgaaa gcatggcggc ctcaagctgg 720

ctggcgctgg gtccgattgg caccggctcg ctcggcatgc tggtcatcgg cggagatgcg 780

cccggggtgt ttgccgcggc gggcatgccg gaaatcggtc acatcgcgca gggcgtgggg 840

ttcgttgccg gcattctctt ctggggctgc ggcctgtggt ggatgctgct ggccacgctg 900

ataaccgtgc gatacttccg tgaaggcatc ccgttcaacc ttggatggtg gggctttacc 960

ttcccgctgg gcgtgtacac cgtggccacg ctcaagctgt ctaccctgat tgacctcgca 1020

ttctttaaag gcttcggtgc gcttcttgtc gtcatactgg cggccatgtg gctgcttgtc 1080

gcggtgaaaa cagtgcgtgg ggcatggagc ggtaaactgt ttgtttctcc ctgtctggca 1140

ggcctggatc ggaagccgtg a 1161

<210> 63

<211> 433

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 63

Met Leu Val Val Glu Leu Ile Ile Val Leu Leu Ala Ile Phe Leu Gly

1 5 10 15

Ala Arg Leu Gly Gly Ile Gly Ile Gly Phe Ala Gly Gly Leu Gly Val

20 25 30

Leu Val Leu Ala Ala Ile Gly Val Lys Pro Gly Asn Ile Pro Phe Asp

35 40 45

Val Ile Ser Ile Ile Met Ala Val Ile Ala Ala Ile Ser Ala Met Gln

50 55 60

Val Ala Gly Gly Leu Asp Tyr Leu Val His Gln Thr Glu Lys Leu Leu

65 70 75 80

Arg Arg Asn Pro Lys Tyr Ile Thr Ile Leu Ala Pro Ile Val Thr Tyr

85 90 95

Phe Leu Thr Ile Phe Ala Gly Thr Gly Asn Ile Ser Leu Ala Thr Leu

100 105 110

Pro Val Ile Ala Glu Val Ala Lys Glu Gln Gly Val Lys Pro Cys Arg

115 120 125

Pro Leu Ser Thr Ala Val Val Ser Ala Gln Ile Ala Ile Thr Ala Ser

130 135 140

Pro Ile Ser Ala Ala Val Val Tyr Met Ser Ser Val Met Glu Gly His

145 150 155 160

Gly Ile Ser Tyr Leu His Leu Leu Ser Val Val Ile Pro Ser Thr Leu

165 170 175

Leu Ala Val Leu Val Met Ser Phe Leu Val Thr Met Leu Phe Asn Ser

180 185 190

Lys Leu Ser Asp Asp Pro Ile Tyr Arg Lys Arg Leu Glu Glu Gly Leu

195 200 205

Val Glu Leu Arg Gly Glu Lys Gln Ile Glu Ile Lys Ser Gly Ala Lys

210 215 220

Thr Ser Val Trp Leu Phe Leu Leu Gly Val Val Gly Val Val Ile Tyr

225 230 235 240

Ala Ile Ile Asn Ser Pro Ser Met Gly Leu Val Glu Lys Pro Leu Met

245 250 255

Asn Thr Thr Asn Ala Ile Leu Ile Ile Met Leu Ser Val Ala Thr Leu

260 265 270

Thr Thr Val Ile Cys Lys Val Asp Thr Asp Asn Ile Leu Asn Ser Ser

275 280 285

Thr Phe Lys Ala Gly Met Ser Ala Cys Ile Cys Ile Leu Gly Val Ala

290 295 300

Trp Leu Gly Asp Thr Phe Val Ser Asn Asn Ile Asp Trp Ile Lys Asp

305 310 315 320

Thr Ala Gly Glu Val Ile Gln Gly His Pro Trp Leu Leu Ala Val Ile

325 330 335

Phe Phe Phe Ala Ser Ala Leu Leu Tyr Ser Gln Ala Ala Thr Ala Lys

340 345 350

Ala Leu Met Pro Met Ala Leu Ala Leu Asn Val Ser Pro Leu Thr Ala

355 360 365

Val Ala Ser Phe Ala Ala Val Ser Gly Leu Phe Ile Leu Pro Thr Tyr

370 375 380

Pro Thr Leu Val Ala Ala Val Gln Met Asp Asp Thr Gly Thr Thr Arg

385 390 395 400

Ile Gly Lys Phe Val Phe Asn His Pro Phe Phe Ile Pro Gly Thr Leu

405 410 415

Gly Val Ala Leu Ala Val Cys Phe Gly Phe Val Leu Gly Ser Phe Met

420 425 430

Leu

<210> 64

<211> 1302

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 64

atgctagttg tagaactcat catagttttg ctggcgatct tcttgggcgc cagattgggg 60

ggaataggta ttggttttgc aggcggattg ggggtgctgg ttcttgccgc tattggcgtt 120

aaacccggta acatcccgtt cgatgtcatc tccattatca tggcggttat cgccgctatt 180

tctgccatgc aggttgctgg cggtctggac tatctggttc atcagacaga aaagctgctg 240

cgccgtaacc cgaaatacat cacgatcctc gcaccgatcg tgacctattt cctgactatc 300

tttgctggta ctggcaacat ctctctggcg acactgccag ttatcgctga agttgcgaag 360

gaacaaggcg ttaaaccttg ccgtccgctg tctactgcag tggtatccgc gcagattgcg 420

atcaccgcat cgccaatctc agcggcagtg gtttacatgt cttccgtgat ggaaggtcat 480

ggcatcagct acctccatct gctctccgtg gtcatcccgt ccaccctgct ggcggttctg 540

gtgatgtcct tcctggtcac tatgctgttc aactccaaac tctctgacga tccgatttat 600

cgcaagcgtc tggaagaggg cctggttgaa ctgcgcggtg aaaagcagat tgaaatcaaa 660

tccggtgcaa aaacgtccgt ctggctgttc ctgctgggcg tagttggcgt ggttatctat 720

gcaatcatca acagcccaag catgggtctg gttgaaaaac cgctgatgaa caccaccaac 780

gcaatcctga tcatcatgct cagcgttgca actctgacca ccgttatctg taaagtcgat 840

accgacaaca tcctcaactc cagcaccttc aaagcaggta tgagcgcctg tatttgtatc 900

ctgggtgttg cgtggctggg cgatactttc gtttccaaca acatcgactg gatcaaagat 960

accgctggtg aagtgattca gggtcatccg tggctgctgg ccgtcatctt cttctttgct 1020

tctgctctgc tgtactctca ggctgcaacc gcaaaagcac tgatgccgat ggctctggca 1080

ctgaacgttt caccgctgac cgctgttgct tctttcgctg cggtgtctgg tctgttcatt 1140

ctgccgacct acccgacgct ggttgctgcg gtacagatgg atgacacggg tactacccgt 1200

atcggtaaat tcgtcttcaa ccatccgttc ttcatcccgg gtactctggg tgttgccctg 1260

gccgtttgct tcggcttcgt gctgggtagc ttcatgctgt aa 1302

<210> 65

<211> 446

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 65

Met Leu Phe Thr Ile Gln Leu Ile Ile Ile Leu Ile Cys Leu Phe Tyr

1 5 10 15

Gly Ala Arg Lys Gly Gly Ile Ala Leu Gly Leu Leu Gly Gly Ile Gly

20 25 30

Leu Val Ile Leu Val Phe Val Phe His Leu Gln Pro Gly Lys Pro Pro

35 40 45

Val Asp Val Met Leu Val Ile Ile Ala Val Val Ala Ala Ser Ala Thr

50 55 60

Leu Gln Ala Ser Gly Gly Leu Asp Val Met Leu Gln Ile Ala Glu Lys

65 70 75 80

Leu Leu Arg Arg Asn Pro Lys Tyr Val Ser Ile Val Ala Pro Phe Val

85 90 95

Thr Cys Thr Leu Thr Ile Leu Cys Gly Thr Gly His Val Val Tyr Thr

100 105 110

Ile Leu Pro Ile Ile Tyr Asp Val Ala Ile Lys Asn Asn Ile Arg Pro

115 120 125

Glu Arg Pro Met Ala Ala Ser Ser Ile Gly Ala Gln Met Gly Ile Ile

130 135 140

Ala Ser Pro Val Ser Val Ala Val Val Ser Leu Val Ala Met Leu Gly

145 150 155 160

Asn Val Thr Phe Asp Gly Arg His Leu Glu Phe Leu Asp Leu Leu Ala

165 170 175

Ile Thr Ile Pro Ser Thr Leu Ile Gly Ile Leu Ala Ile Gly Ile Phe

180 185 190

Ser Trp Phe Arg Gly Lys Asp Leu Asp Lys Asp Glu Glu Phe Gln Lys

195 200 205

Phe Ile Ser Val Pro Glu Asn Arg Glu Tyr Val Tyr Gly Asp Thr Ala

210 215 220

Thr Leu Leu Asp Lys Lys Leu Pro Lys Ser Asn Trp Leu Ala Met Trp

225 230 235 240

Ile Phe Leu Gly Ala Ile Ala Val Val Ala Leu Leu Gly Ala Asp Ser

245 250 255

Asp Leu Arg Pro Ser Phe Gly Gly Lys Pro Leu Ser Met Val Leu Val

260 265 270

Ile Gln Met Phe Met Leu Leu Thr Gly Ala Leu Ile Ile Ile Leu Thr

275 280 285

Lys Thr Asn Pro Ala Ser Ile Ser Lys Asn Glu Val Phe Arg Ser Gly

290 295 300

Met Ile Ala Ile Val Ala Val Tyr Gly Ile Ala Trp Met Ala Glu Thr

305 310 315 320

Met Phe Gly Ala His Met Ser Glu Ile Gln Gly Val Leu Gly Glu Met

325 330 335

Val Lys Glu Tyr Pro Trp Ala Tyr Ala Ile Val Leu Leu Leu Val Ser

340 345 350

Lys Phe Val Asn Ser Gln Ala Ala Ala Leu Ala Ala Ile Val Pro Val

355 360 365

Ala Leu Ala Ile Gly Val Asp Pro Ala Tyr Ile Val Ala Ser Ala Pro

370 375 380

Ala Cys Tyr Gly Tyr Tyr Ile Leu Pro Thr Tyr Pro Ser Asp Leu Ala

385 390 395 400

Ala Ile Gln Phe Asp Arg Ser Gly Thr Thr His Ile Gly Arg Phe Val

405 410 415

Ile Asn His Ser Phe Ile Leu Pro Gly Leu Ile Gly Val Ser Val Ser

420 425 430

Cys Val Phe Gly Trp Ile Phe Ala Ala Met Tyr Gly Phe Leu

435 440 445

<210> 66

<211> 1341

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 66

atgttattta ctatccaact tatcataata ctgatatgtc tgttttatgg tgccagaaag 60

ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc 120

caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg 180

gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag 240

ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg 300

accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc 360

gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag 420

atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt 480

aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca 540

tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg 600

gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac 660

ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg 720

attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca 780

tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc 840

ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc 900

ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc 960

atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat 1020

ccgtgggcct atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg 1080

gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg 1140

gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca 1200

gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagc 1260

tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc 1320

gcgatgtacg ggttcttata a 1341

<210> 67

<211> 461

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 67

Met Leu Thr Phe Ile Glu Leu Leu Ile Gly Val Val Val Ile Val Gly

1 5 10 15

Val Ala Arg Tyr Ile Ile Lys Gly Tyr Ser Ala Thr Gly Val Leu Phe

20 25 30

Val Gly Gly Leu Leu Leu Leu Ile Ile Ser Ala Ile Met Gly His Lys

35 40 45

Val Leu Pro Ser Ser Gln Ala Ser Thr Gly Tyr Ser Ala Thr Asp Ile

50 55 60

Val Glu Tyr Val Lys Ile Leu Leu Met Ser Arg Gly Gly Asp Leu Gly

65 70 75 80

Met Met Ile Met Met Leu Cys Gly Phe Ala Ala Tyr Met Thr His Ile

85 90 95

Gly Ala Asn Asp Met Val Val Lys Leu Ala Ser Lys Pro Leu Gln Tyr

100 105 110

Ile Asn Ser Pro Tyr Leu Leu Met Ile Ala Ala Tyr Phe Val Ala Cys

115 120 125

Leu Met Ser Leu Ala Val Ser Ser Ala Thr Gly Leu Gly Val Leu Leu

130 135 140

Met Ala Thr Leu Phe Pro Val Met Val Asn Val Gly Ile Ser Arg Gly

145 150 155 160

Ala Ala Ala Ala Ile Cys Ala Ser Pro Ala Ala Ile Ile Leu Ala Pro

165 170 175

Thr Ser Gly Asp Val Val Leu Ala Ala Gln Ala Ser Glu Met Ser Leu

180 185 190

Ile Asp Phe Ala Phe Lys Thr Thr Leu Pro Ile Ser Ile Ala Ala Ile

195 200 205

Ile Gly Met Ala Ile Ala His Phe Phe Trp Gln Arg Tyr Leu Asp Lys

210 215 220

Lys Glu His Ile Ser His Glu Met Leu Asp Val Ser Glu Ile Thr Thr

225 230 235 240

Thr Ala Pro Ala Phe Tyr Ala Ile Leu Pro Phe Thr Pro Ile Ile Gly

245 250 255

Val Leu Ile Phe Asp Gly Lys Trp Gly Pro Gln Leu His Ile Ile Thr

260 265 270

Ile Leu Val Ile Cys Met Leu Ile Ala Ser Ile Leu Glu Phe Leu Arg

275 280 285

Ser Phe Asn Thr Gln Lys Val Phe Ser Gly Leu Glu Val Ala Tyr Arg

290 295 300

Gly Met Ala Asp Ala Phe Ala Asn Val Val Met Leu Leu Val Ala Ala

305 310 315 320

Gly Val Phe Ala Gln Gly Leu Ser Thr Ile Gly Phe Ile Gln Ser Leu

325 330 335

Ile Ser Ile Ala Thr Ser Phe Gly Ser Ala Ser Ile Ile Leu Met Leu

340 345 350

Val Leu Val Ile Leu Thr Met Leu Ala Ala Val Thr Thr Gly Ser Gly

355 360 365

Asn Ala Pro Phe Tyr Ala Phe Val Glu Met Ile Pro Lys Leu Ala His

370 375 380

Ser Ser Gly Ile Asn Pro Ala Tyr Leu Thr Ile Pro Met Leu Gln Ala

385 390 395 400

Ser Asn Leu Gly Arg Thr Leu Ser Pro Val Ser Gly Val Val Val Ala

405 410 415

Val Ala Gly Met Ala Lys Ile Ser Pro Phe Glu Val Val Lys Arg Thr

420 425 430

Ser Val Pro Val Leu Val Gly Leu Val Ile Val Ile Val Ala Thr Glu

435 440 445

Leu Met Val Pro Gly Thr Ala Ala Ala Val Thr Gly Lys

450 455 460

<210> 68

<211> 1386

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 68

atgctgacat tcattgagct ccttattggg gttgtggtta ttgtgggtgt agctcgctac 60

atcattaaag ggtattccgc cactggtgtg ttatttgtcg gtggcctgtt attgctgatt 120

atcagtgcca ttatggggca caaagtgtta ccgtccagcc aggcttcaac aggctacagc 180

gccacggata tcgttgaata cgttaaaata ttactaatga gccgcggcgg cgacctcggc 240

atgatgatta tgatgctgtg tggatttgcc gcttacatga cccatatcgg cgcgaatgat 300

atggtggtca agctggcgtc aaaaccattg cagtatatta actcccctta cctgctgatg 360

attgccgcct attttgtcgc ctgtctgatg tctctggccg tctcttccgc aaccggtctg 420

ggtgttttgc tgatggcaac cctatttccg gtgatggtaa acgttggtat cagtcgtggc 480

gcagctgctg ccatttgtgc ctccccggcg gcgattattc tcgcaccgac ttcaggggat 540

gtggtgctgg cggcgcaagc ttccgaaatg tcgctgattg acttcgcctt caaaacgacg 600

ctgcctatct caattgctgc aattatcggc atggcgatcg cccacttctt ctggcaacgt 660

tatctggata aaaaagagca catctctcat gaaatgttag atgtcagtga aatcaccacc 720

actgctcctg cgttttatgc cattttgccg ttcacgccga tcatcggtgt actgattttt 780

gacggtaaat ggggtccgca attacacatc atcactattc tggtgatttg tatgctgatt 840

gcctccattc tggagttcct ccgcagcttt aatacccaga aagttttctc tggtctggaa 900

gtggcttatc gcgggatggc agatgcgttt gctaacgtgg tgatgctgct ggttgccgct 960

ggggtattcg ctcaggggct tagcaccatc ggctttattc aaagtctgat ttctatcgct 1020

acctcgtttg gttcggcgag tatcatcctg atgctggtat tggtgattct gacaatgctg 1080

gcggcagtca cgaccggttc aggcaatgcg ccgttttatg cgtttgttga gatgatcccg 1140

aaactggcgc actcttccgg cattaacccg gcgtatttga ctatcccgat gctgcaggcg 1200

tcaaaccttg gccgtaccct ttcgcccgtt tctggcgtag tcgttgcggt tgccgggatg 1260

gcgaagatct cgccgtttga agtcgtaaaa cgcacctcgg taccggtgct tgttggtttg 1320

gtgattgtta tcgttgctac agagctgatg gtgccaggaa cggcagcagc ggtcacaggc 1380

aagtaa 1386

<210> 69

<211> 310

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 69

Met Lys Thr Leu Ile Arg Lys Phe Ser Arg Thr Ala Ile Thr Val Val

1 5 10 15

Leu Val Ile Leu Ala Phe Ile Ala Ile Phe Asn Ala Trp Val Tyr Tyr

20 25 30

Thr Glu Ser Pro Trp Thr Arg Asp Ala Arg Phe Ser Ala Asp Val Val

35 40 45

Ala Ile Ala Pro Asp Val Ser Gly Leu Ile Thr Gln Val Asn Val His

50 55 60

Asp Asn Gln Leu Val Lys Lys Gly Gln Ile Leu Phe Thr Ile Asp Gln

65 70 75 80

Pro Arg Tyr Gln Lys Ala Leu Glu Glu Ala Gln Ala Asp Val Ala Tyr

85 90 95

Tyr Gln Val Leu Ala Gln Glu Lys Arg Gln Glu Ala Gly Arg Arg Asn

100 105 110

Arg Leu Gly Val Gln Ala Met Ser Arg Glu Glu Ile Asp Gln Ala Asn

115 120 125

Asn Val Leu Gln Thr Val Leu His Gln Leu Ala Lys Ala Gln Ala Thr

130 135 140

Arg Asp Leu Ala Lys Leu Asp Leu Glu Arg Thr Val Ile Arg Ala Pro

145 150 155 160

Ala Asp Gly Trp Val Thr Asn Leu Asn Val Tyr Thr Gly Glu Phe Ile

165 170 175

Thr Arg Gly Ser Thr Ala Val Ala Leu Val Lys Gln Asn Ser Phe Tyr

180 185 190

Val Leu Ala Tyr Met Glu Glu Thr Lys Leu Glu Gly Val Arg Pro Gly

195 200 205

Tyr Arg Ala Glu Ile Thr Pro Leu Gly Ser Asn Lys Val Leu Lys Gly

210 215 220

Thr Val Asp Ser Val Ala Ala Gly Val Thr Asn Ala Ser Ser Thr Arg

225 230 235 240

Asp Asp Lys Gly Met Ala Thr Ile Asp Ser Asn Leu Glu Trp Val Arg

245 250 255

Leu Ala Gln Arg Val Pro Val Arg Ile Arg Leu Asp Asn Gln Gln Glu

260 265 270

Asn Ile Trp Pro Ala Gly Thr Thr Ala Thr Val Val Val Thr Gly Lys

275 280 285

Gln Asp Arg Asp Glu Ser Gln Asp Ser Phe Phe Arg Lys Met Ala His

290 295 300

Arg Leu Arg Glu Phe Gly

305 310

<210> 70

<211> 933

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 70

gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg 60

gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac 120

gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag 180

gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag 240

ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg 300

gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct 360

cgcgaagaga tcgaccaggc caacaacgta ctacaaacgg ttctgcatca gttagcgaaa 420

gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgcgcgcca 480

gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca 540

acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact 600

aagctggaag gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa 660

gtgctgaaag ggactgttga tagtgttgcc gcaggggtca ccaacgccag cagcacgcgt 720

gacgacaaag ggatggcgac tatagactct aaccttgaat gggtgcgtct tgcgcaacgt 780

gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact 840

gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt 900

aaaatggccc atcgcctgcg tgagtttggt taa 933

<210> 71

<211> 655

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 71

Met Gly Ile Phe Ser Ile Ala Asn Gln His Ile Arg Phe Ala Val Lys

1 5 10 15

Leu Ala Thr Ala Ile Val Leu Ala Leu Phe Val Gly Phe His Phe Gln

20 25 30

Leu Glu Thr Pro Arg Trp Ala Val Leu Thr Ala Ala Ile Val Ala Ala

35 40 45

Gly Thr Ala Phe Ala Ala Gly Gly Glu Pro Tyr Ser Gly Ala Ile Arg

50 55 60

Tyr Arg Gly Phe Leu Arg Ile Ile Gly Thr Phe Ile Gly Cys Ile Ala

65 70 75 80

Gly Leu Val Ile Ile Ile Ala Met Ile Arg Ala Pro Leu Leu Met Ile

85 90 95

Leu Val Cys Cys Ile Trp Ala Gly Phe Cys Thr Trp Ile Ser Ser Leu

100 105 110

Val Arg Ile Glu Asn Ser Tyr Ala Trp Gly Leu Ala Gly Tyr Thr Ala

115 120 125

Leu Ile Ile Val Ile Thr Ile Gln Pro Glu Pro Leu Leu Thr Pro Gln

130 135 140

Phe Ala Val Glu Arg Cys Ser Glu Ile Val Ile Gly Ile Val Cys Ala

145 150 155 160

Ile Met Ala Asp Leu Leu Phe Ser Pro Arg Ser Ile Lys Gln Glu Val

165 170 175

Asp Arg Glu Leu Glu Ser Leu Leu Val Ala Gln Tyr Gln Leu Met Gln

180 185 190

Leu Cys Ile Lys His Gly Asp Gly Glu Val Val Asp Lys Ala Trp Gly

195 200 205

Asp Leu Val Arg Arg Thr Thr Ala Leu Gln Gly Met Arg Ser Asn Leu

210 215 220

Asn Met Glu Ser Ser Arg Trp Ala Arg Ala Asn Arg Arg Leu Lys Ala

225 230 235 240

Ile Asn Thr Leu Ser Leu Thr Leu Ile Thr Gln Ser Cys Glu Thr Tyr

245 250 255

Leu Ile Gln Asn Thr Arg Pro Glu Leu Ile Thr Asp Thr Phe Arg Glu

260 265 270

Phe Phe Asp Thr Pro Val Glu Thr Ala Gln Asp Val His Lys Gln Leu

275 280 285

Lys Arg Leu Arg Arg Val Ile Ala Trp Thr Gly Glu Arg Glu Thr Pro

290 295 300

Val Thr Ile Tyr Ser Trp Val Ala Ala Ala Thr Arg Tyr Gln Leu Leu

305 310 315 320

Lys Arg Gly Val Ile Ser Asn Thr Lys Ile Asn Ala Thr Glu Glu Glu

325 330 335

Ile Leu Gln Gly Glu Pro Glu Val Lys Val Glu Ser Ala Glu Arg His

340 345 350

His Ala Met Val Asn Phe Trp Arg Thr Thr Leu Ser Cys Ile Leu Gly

355 360 365

Thr Leu Phe Trp Leu Trp Thr Gly Trp Thr Ser Gly Ser Gly Ala Met

370 375 380

Val Met Ile Ala Val Val Thr Ser Leu Ala Met Arg Leu Pro Asn Pro

385 390 395 400

Arg Met Val Ala Ile Asp Phe Ile Tyr Gly Thr Leu Ala Ala Leu Pro

405 410 415

Leu Gly Leu Leu Tyr Phe Leu Val Ile Ile Pro Asn Thr Gln Gln Ser

420 425 430

Met Leu Leu Leu Cys Ile Ser Leu Ala Val Leu Gly Phe Phe Leu Gly

435 440 445

Ile Glu Val Gln Lys Arg Arg Leu Gly Ser Met Gly Ala Leu Ala Ser

450 455 460

Thr Ile Asn Ile Ile Val Leu Asp Asn Pro Met Thr Phe His Phe Ser

465 470 475 480

Gln Phe Leu Asp Ser Ala Leu Gly Gln Ile Val Gly Cys Val Leu Ala

485 490 495

Phe Thr Val Ile Leu Leu Val Arg Asp Lys Ser Arg Asp Arg Thr Gly

500 505 510

Arg Val Leu Leu Asn Gln Phe Val Ser Ala Ala Val Ser Ala Met Thr

515 520 525

Thr Asn Val Ala Arg Arg Lys Glu Asn His Leu Pro Ala Leu Tyr Gln

530 535 540

Gln Leu Phe Leu Leu Met Asn Lys Phe Pro Gly Asp Leu Pro Lys Phe

545 550 555 560

Arg Leu Ala Leu Thr Met Ile Ile Ala His Gln Arg Leu Arg Asp Ala

565 570 575

Pro Ile Pro Val Asn Glu Asp Leu Ser Ala Phe His Arg Gln Met Arg

580 585 590

Arg Thr Ala Asp His Val Ile Ser Ala Arg Ser Asp Asp Lys Arg Arg

595 600 605

Arg Tyr Phe Gly Gln Leu Leu Glu Glu Leu Glu Ile Tyr Gln Glu Lys

610 615 620

Leu Arg Ile Trp Gln Ala Pro Pro Gln Val Thr Glu Pro Val Asn Arg

625 630 635 640

Leu Ala Gly Met Leu His Lys Tyr Gln His Ala Leu Thr Asp Ser

645 650 655

<210> 72

<211> 1968

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 72

atgggtattt tctccattgc taaccaacat attcgctttg cggtaaaact ggcgaccgcc 60

attgtactgg cgctgtttgt tggctttcac ttccagctgg aaacgccacg ctgggcggta 120

ctgacagcgg cgattgttgc cgccggtacg gcctttgctg cgggaggtga accgtattct 180

ggcgctattc gctatcgtgg ctttttgcgc atcatcggca catttattgg ctgtattgcc 240

ggactggtga tcatcattgc gatgatccgc gcaccattat tgatgattct ggtgtgctgt 300

atctgggccg gtttttgtac ctggatatcc tcgctggtac gaatagaaaa ctcgtatgcg 360

tgggggctgg ccggttatac cgcgctgatc attgtgatca ccattcagcc ggaaccattg 420

cttacgccgc agtttgccgt cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgcg 480

attatggcgg atttgctctt ttctccgcga tcgatcaaac aagaagtgga tcgagagctg 540

gaaagtttgc tggtcgcgca atatcaatta atgcaactct gtatcaagca tggcgatggt 600

gaagttgtcg ataaagcctg gggcgacctg gtgcgacgca ccacggcgct acaaggcatg 660

cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg ccaatcgacg tttaaaagcg 720

atcaatacgc tatcgctgac gctgattacc caatcctgcg aaacttatct tattcagaat 780

acgcgcccgg aattgatcac tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc 840

gcgcaggacg tccacaagca gctcaaacgc ctgcggagag ttatcgcctg gaccggggaa 900

cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc 960

aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg aagaagagat cctgcaaggc 1020

gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg caatggttaa cttctggcga 1080

accacacttt cctgcattct gggcacgctt ttctggctgt ggacgggctg gacttccggc 1140

agtggtgcaa tggtgatgat tgcggtagtg acgtcactgg caatgcgttt gccgaatcca 1200

cgcatggtgg cgatcgactt tatctacggg acgctggccg cgctgccgtt agggctgctc 1260

tactttttgg tgattatccc taatacccaa cagagcatgt tgctgctgtg cattagcctg 1320

gcagtgctgg gattcttcct cggtatagaa gtacagaaac ggcgactggg ctcgatgggg 1380

gcactggcca gcaccataaa tattatcgtg ctggataacc cgatgacttt ccatttcagt 1440

cagtttctcg acagcgcatt agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt 1500

ttgctggtgc gggataaatc gcgcgacagg accggacgtg tactgcttaa tcagtttgtt 1560

tctgccgctg tttccgcgat gactaccaat gtggcacgtc gtaaagagaa ccacctcccg 1620

gcactttatc agcagctgtt tttgctgatg aataagttcc caggggattt gccgaaattt 1680

cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc gtgatgcacc gatcccggtt 1740

aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca cagcagacca tgtgatatct 1800

gcccgtagcg atgataaacg tcgtcggtac tttggccagt tgctggaaga actggaaatc 1860

taccaggaaa agctacgcat ctggcaagcg ccaccgcagg tgacggaacc ggtaaatcgg 1920

ctggcgggga tgctccataa gtatcaacat gcgttgaccg atagttaa 1968

<210> 73

<211> 151

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 73

Met Ala Glu Ser Thr Val Thr Ala Asp Ser Lys Leu Thr Ser Ser Asp

1 5 10 15

Thr Arg Arg Arg Ile Trp Ala Ile Val Gly Ala Ser Ser Gly Asn Leu

20 25 30

Val Glu Trp Phe Asp Phe Tyr Val Tyr Ser Phe Cys Ser Leu Tyr Phe

35 40 45

Ala His Ile Phe Phe Pro Ser Gly Asn Thr Thr Thr Gln Leu Leu Gln

50 55 60

Thr Ala Gly Val Phe Ala Ala Gly Phe Leu Met Arg Pro Ile Gly Gly

65 70 75 80

Trp Leu Phe Gly Arg Ile Ala Asp Lys His Gly Arg Lys Lys Ser Met

85 90 95

Leu Leu Ser Val Cys Met Met Cys Phe Gly Ser Leu Val Ile Ala Cys

100 105 110

Leu Pro Gly Tyr Glu Thr Ile Gly Thr Trp Ala Pro Ala Leu Leu Leu

115 120 125

Leu Ala Arg Leu Phe Gln Gly Leu Ser Val Gly Gly Glu Tyr Gly Thr

130 135 140

Ser Ala Thr Tyr Met Ser Glu

145 150

<210> 74

<211> 1299

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 74

atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac tcgtcgccgc 60

atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga tttctatgtc 120

tactcgttct gttcactcta ctttgcccac atcttcttcc cttccgggaa cacgacgact 180

caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc aataggcggt 240

tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct gttatcggtg 300

tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga aactataggt 360

acgtgggctc cggcattatt gcttctcgct cgtttatttc agggattatc tgttggcgga 420

gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg caaaggtttt 480

tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct actggttgtc 540

gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg gcgtattcct 600

ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca gttagatgaa 660

acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg attatggcgc 720

aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct ttgtttctat 780

accttcacta cttatatgca gaagtatctg gtaaatactg cgggaatgca tgccaacgtg 840

gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc actcattggc 900

gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc gctggcagcc 960

atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta tgccgctttt 1020

ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag tggaatactg 1080

aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc atatgcggtc 1140

gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa atcaatagga 1200

atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt tctggtttct 1260

ttgatgctac atcgcaaagg gaaggggatg cgtctttag 1299

<210> 75

<211> 161

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 75

Met Asn Leu Trp Gln Gln Asn Tyr Asp Pro Ala Gly Asn Ile Trp Leu

1 5 10 15

Ser Ser Leu Ile Ala Ser Leu Pro Ile Leu Phe Phe Phe Phe Ala Leu

20 25 30

Ile Lys Leu Lys Leu Lys Gly Tyr Val Ala Ala Ser Trp Thr Val Ala

35 40 45

Ile Ala Leu Ala Val Ala Leu Leu Phe Tyr Lys Met Pro Val Ala Asn

50 55 60

Ala Leu Ala Ser Val Val Tyr Gly Phe Phe Tyr Gly Leu Trp Pro Ile

65 70 75 80

Ala Trp Ile Ile Ile Ala Ala Val Phe Val Tyr Lys Ile Ser Val Lys

85 90 95

Thr Gly Gln Phe Asp Ile Ile Arg Ser Ser Ile Leu Ser Ile Thr Pro

100 105 110

Asp Gln Arg Leu Gln Met Leu Ile Val Gly Phe Cys Phe Gly Ala Phe

115 120 125

Leu Glu Gly Ala Ala Gly Phe Gly Ala Pro Val Ala Ile Thr Ala Ala

130 135 140

Leu Leu Val Gly Leu Gly Phe Lys Pro Leu Tyr Ala Ala Gly Leu Cys

145 150 155 160

Leu

<210> 76

<211> 1656

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 76

atgaatctct ggcaacaaaa ctacgatccc gccgggaata tctggctttc cagtctgata 60

gcatcgcttc ccatcctgtt tttcttcttt gcgctgatta agctcaaact gaaaggatac 120

gtcgccgcct cgtggacggt ggcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg 180

ccggtcgcta acgcgctggc ctcggtggtt tatggtttct tctacgggtt gtggcccatc 240

gcgtggatca ttattgcagc ggtgttcgtc tataagatct cggtgaaaac cgggcagttt 300

gacatcattc gctcgtctat tctttcgata acccctgacc agcgtctgca aatgctgatc 360

gtcggtttct gtttcggcgc gttccttgaa ggagccgcag gctttggcgc accggtagca 420

attaccgccg cattgctggt cggcctgggt tttaaaccgc tgtacgccgc cgggctgtgc 480

ctgattgtta acaccgcgcc agtggcattt ggtgcgatgg gcattccaat cctggttgcc 540

ggacaggtaa caggtatcga cagctttgag attggtcaga tggtggggcg gcagctaccg 600

tttatgacca ttatcgtgct gttctggatc atggcgatta tggacggctg gcgcggtatc 660

aaagagacgt ggcctgcggt cgtggttgcg ggcggctcgt ttgccatcgc tcagtacctt 720

agctctaact tcattgggcc ggagctgccg gacattatct cttcgctggt atcactgctc 780

tgcctgacgc tgttcctcaa acgctggcag ccagtgcgtg tattccgttt tggtgatttg 840

ggggcgtcac aggttgatat gacgctggcc cacaccggtt acactgcggg tcaggtgtta 900

cgtgcctgga caccgttcct gttcctgaca gctaccgtaa cactgtggag tatcccgccg 960

tttaaagccc tgttcgcatc gggtggcgcg ctgtatgagt gggtgatcaa tattccggtg 1020

ccgtacctcg ataaactggt tgcccgtatg ccgccagtgg tcagcgaggc tacagcctat 1080

gccgccgtgt ttaagtttga ctggttctct gccaccggca ccgccattct gtttgctgca 1140

ctgctctcga ttgtctggct gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc 1200

acgctgaaag aactggctct gcccatctac tccatcggta tggtgctggc attcgccttt 1260

atttcgaact attccggact gtcatcaaca ctggcgctgg cactggcgca caccggtcat 1320

gcattcacct tcttctcgcc gttcctcggc tggctggggg tattcctgac cgggtcggat 1380

acctcatcta acgccctgtt cgccgcgctg caagccaccg cagcacaaca aattggcgtc 1440

tctgatctgt tgctggttgc cgccaatacc accggtggcg tcaccggtaa gatgatctcc 1500

ccgcaatcta tcgctatcgc ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg 1560

ttccgcttta ctgtcaaaca cagcctgatc ttcacctgta tagtgggcgt gatcaccacg 1620

cttcaggctt atgtcttaac gtggatgatt ccttaa 1656

<210> 77

<211> 156

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 77

Met Val Thr Trp Thr Gln Met Tyr Met Pro Met Gly Gly Leu Gly Leu

1 5 10 15

Ser Ala Leu Val Ala Leu Ile Pro Ile Ile Phe Phe Phe Val Ala Leu

20 25 30

Ala Val Leu Arg Leu Lys Gly His Val Ala Gly Ala Ile Thr Leu Ile

35 40 45

Leu Ser Ile Leu Ile Ala Ile Phe Ala Phe Lys Met Pro Ile Asp Met

50 55 60

Ala Phe Ala Ala Ala Gly Tyr Gly Phe Ile Tyr Gly Leu Trp Pro Ile

65 70 75 80

Ala Trp Ile Ile Val Ala Ala Val Phe Leu Tyr Lys Leu Thr Val Ala

85 90 95

Ser Gly Gln Phe Asp Ile Ile Arg Ser Ser Val Ile Ser Ile Thr Asp

100 105 110

Asp Gln Arg Leu Gln Val Leu Leu Ile Gly Phe Ser Phe Gly Ala Leu

115 120 125

Leu Glu Gly Ala Ala Gly Phe Gly Ala Pro Val Ala Ile Thr Gly Ala

130 135 140

Leu Leu Val Gly Leu Gly Phe Lys Pro Leu Tyr Ala

145 150 155

<210> 78

<211> 1683

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 78

atggttacct ggacccaaat gtatatgccg atgggaggac tggggctatc cgctctggtc 60

gccctgatcc cgataatatt cttcttcgtt gcactcgcgg tattacgtct gaaaggacat 120

gtcgctggag caataaccct tatattatct atcctgattg caatattcgc ctttaaaatg 180

ccgattgata tggcatttgc tgctgcgggc tatggcttta tttatggatt atggccaata 240

gcgtggatta ttgtcgcggc ggtgttcctg tataaattaa ccgttgccag cgggcagttc 300

gatattatcc gcagctcggt tatctccatc accgacgatc agcgtttgca ggtgttactg 360

attggtttct cctttggtgc gttgctggaa ggagcggctg gctttggtgc gccggtggcg 420

attaccggtg cgctgctggt gggcctgggc ttcaaaccgt tatacgcggc ggggctgtgt 480

ctgattgcca atactgcgcc ggtggcgttt ggtgcgttgg gcgtgccgat tctggtcgcc 540

ggtcaggtaa cgggaatcga tccgttccac attggcgcaa tggcgggacg tcagttaccg 600

ttcctgtcgg ttcttgtgcc gttctggctg gtagcaatga tggacggctg gaaaggggtg 660

aaagagacgt ggccagcggc gctggttgct gggggaagct tcgctgtcac tcagttcttt 720

acctctaact atattggtcc ggaactgccg gatattactt cggcgctggt gagtatcgtc 780

tcactcgctt tattccttaa agtctggcgg ccgaaaaata ccgaaacggc aatcagcatg 840

ggacaatccg caggtgcgat ggtggtaaat aagccatctt ctggcggtcc cgtgccttca 900

gaatatagtc tggggcaaat cattcgagcg tggtcaccgt ttttaatctt aacggtgctg 960

gtcaccatct ggaccatgaa gccgtttaaa gcgttatttg ctccgggcgg cgcgttttat 1020

tcactggtga ttaatttcca gatccctcat ttgcatcaac aagtgttgaa agcggcaccc 1080

attgtcgccc aaccaacgcc aatggatgcg gtgtttaaat tcgaccccct ctcggctggc 1140

ggcaccgcta tttttattgc ggcgattatc tctatcttca tcctcggtgt ggggatcaag 1200

aaaggtattg gcgtctttgc cgaaacgcta attagcttga agtggccgat actgtcgatt 1260

ggcatggtgc tggcgttcgc cttcgtcacc aactattctg gcatgtccac cacgctggcg 1320

ctggtactgg caggtacagg cgtgatgttc ccgttcttct caccgtttct cggctggctg 1380

ggcgtattcc ttaccggctc ggacacctcc tctaacgccc tgtttggttc actgcaatcg 1440

accacggcgc agcaaatcaa cgtctctgac accctgctgg tggcagcaaa caccagcggc 1500

ggcgtaactg gcaagatgat ctccccgcaa tctatcgccg tggcctgcgc cgcgacgggc 1560

atggtgggcc gagaatctga actgttccgc tacaccgtga agcacagtct gatttttgcc 1620

agcgttatcg gcattatcac cctgctgcag gcgtatgtgt ttaccgggat gttagtctcg 1680

taa 1683

<210> 79

<211> 150

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 79

Met Lys Arg Val Leu Thr Ala Leu Ala Ala Thr Leu Pro Phe Ala Ala

1 5 10 15

Asn Ala Ala Asp Ala Ile Ser Gly Ala Val Glu Arg Gln Pro Thr Asn

20 25 30

Trp Gln Ala Ile Ile Met Phe Leu Ile Phe Val Val Phe Thr Leu Gly

35 40 45

Ile Thr Tyr Trp Ala Ser Lys Arg Val Arg Ser Arg Ser Asp Tyr Tyr

50 55 60

Thr Ala Gly Gly Asn Ile Thr Gly Phe Gln Asn Gly Leu Ala Ile Ala

65 70 75 80

Gly Asp Tyr Met Ser Ala Ala Ser Phe Leu Gly Ile Ser Ala Leu Val

85 90 95

Phe Thr Ser Gly Tyr Asp Gly Leu Ile Tyr Ser Leu Gly Phe Leu Val

100 105 110

Gly Trp Pro Ile Ile Leu Phe Leu Ile Ala Glu Arg Leu Arg Asn Leu

115 120 125

Gly Arg Tyr Thr Phe Ala Asp Val Ala Ser Tyr Arg Leu Lys Gln Gly

130 135 140

Pro Ile Arg Ile Leu Ser

145 150

<210> 80

<211> 1650

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 80

atgaaaagag ttctgacggc gcttgccgcc acactccctt tcgcagctaa cgccgcggat 60

gctattagcg gggccgtaga gcgccagcca acgaactggc aggcgattat tatgttcctg 120

attttcgtcg tgtttacgct cggcattacc tactgggcat caaaacgcgt acgttctcgt 180

agcgactact acaccgcagg cggcaatatc actggcttcc agaacgggct ggcgattgcc 240

ggggactata tgtccgccgc ctcattcttg gggatctccg cgctggtgtt tacctccggc 300

tatgacggct taatttactc gctgggcttc ctggtgggct ggccgatcat tttgttcctg 360

attgccgaac gtctgcgtaa cctggggcgc tacacctttg ccgatgtggc ctcttaccgt 420

ctgaaacaag ggccgattcg tattctttcg gcctgtggtt ctctggtggt ggtggcgctt 480

taccttatcg cccagatggt gggcgcaggt aaactgatcg agctgctgtt tggccttaac 540

tatcacattg cggtggtgct ggtcggcgtg ctgatgatga tgtacgtcct gttcggcggc 600

atgctggcga ccacctgggt gcaaattatc aaagccgtgc tgttgctgtt cggtgccagc 660

tttatggcct ttatggtgat gaaacacgtc ggctttagct tcaacaatct gttcagtgaa 720

gcgatggcgg tacacccgaa aggtgtcgac atcatgaagc cgggcgggct ggtgaaagat 780

ccgatctccg cgctctctct gggtctggga ctgatgtttg gtacggcggg cttgccgcac 840

attctgatgc gcttctttac agtcagcgat gcccgcgaag cacgtaagag cgtgttctac 900

gccaccgggt ttatgggcta cttctatatt ctgaccttta ttatcggctt cggcgcgatc 960

atgctggttg gtgcgaatcc ggaatataaa gacgcggcgg gccatctgat tggtggtaac 1020

aacatggcgg ccgttcacct ggcgaatgca gtgggcggca acctgttcct cggttttatt 1080

tcagcggttg ctttcgccac tatcctcgcg gtggttgcgg gtctgacgct ggcgggcgca 1140

tccgcggttt cgcatgactt gtacgctaac gtcttcaaaa aaggcgcgac cgaacgtgaa 1200

gagctgcggg tatcaaaaat caccgtactg atcctcggcg tgattgcgat tatcctcggc 1260

gtgctgtttg agaatcagaa catcgccttt atggtggggc tggcgtttgc catcgcggcg 1320

agctgtaact tcccgatcat tctgctttct atgtactggt cgaaactgac cacgcgtggc 1380

gcgatgatgg gtggctggct ggggctgatt accgcagtag tactgatgat cctcggcccg 1440

acgatttggg tacagatcct tggtcacgaa aaagccatct tcccgtatga atacccggcg 1500

ctgttctcta tcaccgtggc attcctcggc atctggttct tctcggcaac cgataactca 1560

gcggaaggcg cgcgtgagcg tgaactgttc cgcgcgcagt ttatccgctc ccagaccggc 1620

tttggcgttg agcaaggccg cgcgcattaa 1650

<210> 81

<211> 152

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 81

Met Asp Arg Phe Pro Arg Ser Asp Ser Ile Val Gln Pro Arg Ala Gly

1 5 10 15

Leu Gln Thr Tyr Met Ala Gln Val Tyr Gly Trp Met Thr Val Gly Leu

20 25 30

Leu Leu Thr Ala Phe Val Ala Trp Tyr Ala Ala Asn Ser Ala Ala Val

35 40 45

Met Glu Leu Leu Phe Thr Asn Arg Val Phe Leu Ile Gly Leu Ile Ile

50 55 60

Ala Gln Leu Ala Leu Val Ile Val Leu Ser Ala Met Ile Gln Lys Leu

65 70 75 80

Ser Ala Gly Val Thr Thr Met Leu Phe Met Leu Tyr Ser Ala Leu Thr

85 90 95

Gly Leu Thr Leu Ser Ser Ile Phe Ile Val Tyr Thr Ala Ala Ser Ile

100 105 110

Ala Ser Thr Phe Val Val Thr Ala Gly Met Phe Gly Ala Met Ser Leu

115 120 125

Tyr Gly Tyr Thr Thr Lys Arg Asp Leu Ser Gly Phe Gly Asn Met Leu

130 135 140

Phe Met Ala Leu Ile Gly Ile Val

145 150

<210> 82

<211> 705

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 82

atggacagat tcccacgttc tgattcaatc gtacaacccc gggctggctt gcaaacttat 60

atggctcaag tctatggctg gatgaccgtt ggcttgttgc tgaccgcatt tgttgcctgg 120

tatgcggcta attccgcggc cgtgatggag ctgttgttca ctaaccgtgt ctttttaatc 180

ggtctgatca tcgcgcaatt agcattggtt attgtgttat cagcgatgat tcaaaagctg 240

agcgcaggtg taacgacgat gctctttatg ctttattcgg cgctgacggg tcttacgctt 300

tccagtatat tcattgtcta taccgctgct tctatcgcca gtactttcgt cgttactgcc 360

gggatgttcg gcgcaatgag cctgtacggt tacaccacga agcgcgattt aagtggcttt 420

ggcaatatgc tgtttatggc gttaatcggc attgtgctgg catcgctggt caacttctgg 480

ttgaaaagcg aagcattgat gtgggcagtt acctacatcg gcgtgattgt ctttgtcgga 540

ttgacggcgt atgacacgca gaaactgaaa aatatgggtg agcagattga tacccgcgac 600

acgtcgaacc tgcgcaaata ttccattctt ggcgcgttaa ccttgtatct ggacttcatc 660

aacctgttcc tgatgttgtt gcggatcttc ggcaaccgcc gttaa 705

<210> 83

<211> 155

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 83

Met Ala Ile Ser Thr Pro Met Leu Val Thr Phe Cys Val Tyr Ile Phe

1 5 10 15

Gly Met Ile Leu Ile Gly Phe Ile Ala Trp Arg Ser Thr Lys Asn Phe

20 25 30

Asp Asp Tyr Ile Leu Gly Gly Arg Ser Leu Gly Pro Phe Val Thr Ala

35 40 45

Leu Ser Ala Gly Ala Ser Asp Met Ser Gly Trp Leu Leu Met Gly Leu

50 55 60

Pro Gly Ala Val Phe Leu Ser Gly Ile Ser Glu Ser Trp Ile Ala Ile

65 70 75 80

Gly Leu Thr Leu Gly Ala Trp Ile Asn Trp Lys Leu Val Ala Gly Arg

85 90 95

Leu Arg Val His Thr Glu Tyr Asn Asn Asn Ala Leu Thr Leu Pro Asp

100 105 110

Tyr Phe Thr Gly Arg Phe Glu Asp Lys Ser Arg Ile Leu Arg Ile Ile

115 120 125

Ser Ala Leu Val Ile Leu Leu Phe Phe Thr Ile Tyr Cys Ala Ser Gly

130 135 140

Ile Val Ala Gly Ala Arg Leu Phe Glu Ser Thr

145 150 155

<210> 84

<211> 1509

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 84

atggctatta gcacaccgat gttggtgaca ttttgtgtct atatctttgg catgatattg 60

attgggttta tcgcctggcg atcaacgaaa aactttgacg actatattct gggcggtcgt 120

agtcttgggc cattcgtgac ggcattatcg gcgggtgcgt cggatatgag cggctggctg 180

ttaatggggt tgccgggcgc tgtttttctt tccgggattt ccgaaagctg gatcgccatt 240

ggcctgacat taggcgcgtg gattaactgg aagctggtgg ccgggcggtt gcgtgtgcat 300

accgaataca acaataacgc cttaacactg ccggattatt tcaccgggcg ctttgaagat 360

aaaagccgca ttttgcgcat tatctctgcg ctggttattt tgctgttctt caccatttat 420

tgcgcttcgg gcattgtggc aggcgcgcgt ctgtttgaaa gtacctttgg catgagctac 480

gaaacggctc tgtgggcggg cgctgcggcg acgatccttt acacctttat tggcggtttc 540

ctcgcggtga gctggactga cactgtacag gccagcctga tgatttttgc cctgatcctg 600

acgccggtta tcgtcattat cagtgtcggt ggctttggtg actcgctgga agtgatcaaa 660

caaaagagca tcgaaaacgt tgatatgctc aaaggtctga actttgttgc cattatctca 720

ctgatgggtt gggggctggg ttacttcggg cagccgcaca ttctggcgcg ttttatggcg 780

gcggattctc accacagcat tgtccatgcg cgtcgtatta gtatgacctg gatgatcctc 840

tgcctggcag gggcggtggc tgtcggcttc tttgggattg cttactttaa cgatcatccg 900

gcgttggctg gtgcggtaaa tcagaacgcc gagcgtgtgt ttatcgaact ggcgcaaatt 960

ctgtttaacc cgtggattgc cgggattctg ctgtcggcaa ttctggcggc ggtaatgtca 1020

accttaagtt gccagctgct ggtgtgctcc agtgcgatta ccgaagattt gtacaaagcg 1080

tttctgcgta aacatgccag ccagaaagag ctggtgtggg tagggcgtgt gatggtgctg 1140

gtggtggcgc tggtggcgat tgcgctggcg gcaaacccgg aaaaccgcgt gctgggctta 1200

gtgagctacg cgtgggcagg ctttggcgcg gcgtttggtc cagtggtgct gttctcggtg 1260

atgtggtcac gcatgacgcg taacggtgcg ctggcgggga tgatcatcgg tgcgctgacg 1320

gttatcgtct ggaaacagtt cggctggctg ggactgtacg aaattattcc gggctttatc 1380

ttcggcagta ttgggattgt agtgtttagt ttgctgggta aagcgccgtc agcggcgatg 1440

caaaaacgct ttgccgaggc cgatgcgcac tatcattcgg ctccgccgtc acggttgcag 1500

gaaagctaa 1509

<210> 85

<211> 150

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 85

Met Gln Leu Glu Val Ile Leu Pro Leu Val Ala Tyr Leu Val Val Val

1 5 10 15

Phe Gly Ile Ser Val Tyr Ala Met Arg Lys Arg Ser Thr Gly Thr Phe

20 25 30

Leu Asn Glu Tyr Phe Leu Gly Ser Arg Ser Met Gly Gly Ile Val Leu

35 40 45

Ala Met Thr Leu Thr Ala Thr Tyr Ile Ser Ala Ser Ser Phe Ile Gly

50 55 60

Gly Pro Gly Ala Ala Tyr Lys Tyr Gly Leu Gly Trp Val Leu Leu Ala

65 70 75 80

Met Ile Gln Leu Pro Ala Val Trp Leu Ser Leu Gly Ile Leu Gly Lys

85 90 95

Lys Phe Ala Ile Leu Ala Arg Arg Tyr Asn Ala Val Thr Leu Asn Asp

100 105 110

Met Leu Phe Ala Arg Tyr Gln Ser Arg Leu Leu Val Trp Leu Ala Ser

115 120 125

Leu Ser Leu Leu Val Ala Phe Val Gly Ala Met Thr Val Gln Phe Ile

130 135 140

Gly Gly Ala Arg Leu Leu

145 150

<210> 86

<211> 1452

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 86

atgcagcttg aagtaattct accgctggtc gcctatctgg tggtggtgtt cggtatctcg 60

gtttatgcga tgcgtaaacg gagcaccggc accttcctta atgagtattt cctcggcagc 120

cgctctatgg gcggtattgt gctggcgatg acgctcaccg cgacctatat cagtgccagt 180

tcgtttatcg gcgggccagg agctgcttat aaatacgggc tgggctgggt attgctggcg 240

atgattcagc ttcctgcagt ctggctttca ctcggtattc tcggcaagaa gtttgcgatt 300

cttgcgcgcc gctacaatgc agtgacgctg aacgatatgc tgtttgcccg ctaccagagt 360

cgtcttctgg tgtggctggc gagtttgagt ttgctggttg cgttcgttgg tgcgatgacc 420

gtgcagttta tcggcggtgc gcgcctgctg gaaaccgcgg cgggtattcc ttatgaaacc 480

gggctgctga tttttggtat cagcattgcg ttatataccg cctttggtgg ctttcgcgcc 540

agcgtgctga acgacaccat gcaagggctt gtgatgctga ttggcaccgt tgtgctgctt 600

attggcgtag tacatgccgc tggcggctta agtaacgcag tacagacctt gcaaaccatc 660

gatccgcaac tggttacgcc acaaggcgct gacgatattc tgtcgcctgc ctttatgacg 720

tcgttctggg tactggtgtg ttttggcgtg attggcctgc cgcatactgc ggtgcgctgt 780

atctcttata aagacagcaa agccgtacat cgggggatca tcatcggtac gattgtggtc 840

gcaattctga tgttcggtat gcacctggcc ggagcgttag gtcgggcggt gatccccgat 900

ctcaccgtac cggacctggt gatcccaacg ttaatggtaa aagtgctgcc accgtttgct 960

gccgggatct tcctggctgc accgatggct gcgatcatgt cgacaattaa cgcccaactg 1020

ctgcaaagtt ccgctacgat cattaaagat ctctatctga atatccgtcc ggatcaaatg 1080

caaaacgaga cgcgtctgaa gcggatgtcg gcggtaatta cgttagttct cggcgcgttg 1140

ctgctgcttg ccgcctggaa gccgccagaa atgatcatct ggctgaattt gttggccttc 1200

ggtgggctgg aagccgtttt cctgtggccg ctggtgctgg gtctttactg ggaacgcgcc 1260

aacgccaaag gcgcgctaag tgcgatgatc gttggcggcg tgctgtatgc cgtactcgcg 1320

acgctgaata ttcagtacct gggcttccac cctatcgtgc cctcgttact actaagtttg 1380

ctggctttcc tggtcggaaa ccgtttcggt acatccgtcc cgcaagctac cgttttgact 1440

actgataaat aa 1452

<210> 87

<211> 140

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 87

Met Gly Asn Thr Lys Leu Ala Asn Pro Ala Pro Leu Gly Leu Met Gly

1 5 10 15

Phe Gly Met Thr Thr Ile Leu Leu Asn Leu His Asn Val Gly Tyr Phe

20 25 30

Ala Leu Asp Gly Ile Ile Leu Ala Met Gly Ile Phe Tyr Gly Gly Ile

35 40 45

Ala Gln Ile Phe Ala Gly Leu Leu Glu Tyr Lys Lys Gly Asn Thr Phe

50 55 60

Gly Leu Thr Ala Phe Thr Ser Tyr Gly Ser Phe Trp Leu Thr Leu Val

65 70 75 80

Ala Ile Leu Leu Met Pro Lys Leu Gly Leu Thr Asp Ala Pro Asn Ala

85 90 95

Gln Phe Leu Gly Val Tyr Leu Gly Leu Trp Gly Val Phe Thr Leu Phe

100 105 110

Met Phe Phe Gly Thr Leu Lys Gly Ala Arg Val Leu Gln Phe Val Phe

115 120 125

Phe Ser Leu Thr Val Leu Phe Ala Leu Leu Ala Ile

130 135 140

<210> 88

<211> 567

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 88

atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc 60

accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat tattcttgcc 120

atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga gtacaaaaaa 180

ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt 240

gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca gttccttggt 300

gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac gctgaaaggc 360

gcacgcgttc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc 420

ggtaacattg ccggtaacgc cgcaatcatc cactttgccg gctggattgg gctgatctgc 480

ggtgccagcg caatctatct ggcgatgggt gaagtactga acgagcagtt tggtcgcacc 540

gttctgccga ttggtgaatc ccactaa 567

<210> 89

<211> 144

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 89

Met Ile Gly Arg Ile Ser Arg Phe Met Thr Arg Phe Val Ser Arg Trp

1 5 10 15

Leu Pro Asp Pro Leu Ile Phe Ala Met Leu Leu Thr Leu Leu Thr Phe

20 25 30

Val Ile Ala Leu Trp Leu Thr Pro Gln Thr Pro Ile Ser Met Val Lys

35 40 45

Met Trp Gly Asp Gly Phe Trp Asn Leu Leu Ala Phe Gly Met Gln Met

50 55 60

Ala Leu Ile Ile Val Thr Gly His Ala Leu Ala Ser Ser Ala Pro Val

65 70 75 80

Lys Ser Leu Leu Arg Thr Ala Ala Ser Ala Ala Lys Thr Pro Val Gln

85 90 95

Gly Val Met Leu Val Thr Phe Phe Gly Ser Val Ala Cys Val Ile Asn

100 105 110

Trp Gly Phe Gly Leu Val Val Gly Ala Met Phe Ala Arg Glu Val Ala

115 120 125

Arg Arg Val Pro Gly Ser Asp Tyr Pro Leu Leu Ile Ala Cys Ala Tyr

130 135 140

<210> 90

<211> 1323

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 90

atgattggtc gcatatcgcg ttttatgacg cgttttgtca gccggtggct tcccgatcca 60

ctgatctttg ccatgttgct gacattgcta acattcgtga tcgcgctttg gttaacacca 120

caaacgccga tcagcatggt gaaaatgtgg ggtgacggtt tctggaactt gctggcgttt 180

ggtatgcaga tggcgcttat catcgttacc ggtcatgccc ttgccagctc tgctccggtg 240

aaaagtttgc tgcgtactgc cgcctccgcc gcaaagacgc ccgtacaggg cgtcatgctg 300

gtcactttct tcggttcagt cgcttgtgtc atcaactggg gatttggttt ggttgtcggc 360

gcaatgtttg cccgtgaagt cgcccggcga gtccccggtt ctgattatcc gttgctcatt 420

gcctgcgcct acattggttt tctcacctgg ggtggcggct tctctggatc aatgcctctg 480

ttggctgcaa caccgggcaa cccggttgag catatcgccg ggctgatccc ggtgggcgat 540

actctgttca gtggttttaa cattttcatc actgtggcgt tgattgtggt gatgccattt 600

atcacccgca tgatgatgcc aaaaccgtct gacgtggtga gtatcgatcc aaaactactc 660

atggaagagg ctgattttca aaagcagcta ccgaaagatg ccccaccatc cgagcgactg 720

gaagaaagcc gcattctgac gttgatcatc ggcgcactcg gtatcgctta ccttgcgatg 780

tacttcagcg aacatggctt caacatcacc atcaataccg tcaacctgat gtttatgatt 840

gcgggtctgc tgctacataa aacgccaatg gcttatatgc gtgctatcag cgcggcagca 900

cgcagtactg ccggtattct ggtgcaattc cccttctacg ctgggatcca actgatgatg 960

gagcattccg gtctgggcgg actcattacc gaattcttca tcaatgttgc gaacaaagac 1020

accttcccgg taatgacctt ttttagttct gcactgatta acttcgccgt tccgtctggc 1080

ggcggtcact gggttattca gggacctttc gtgatacccg cagcccaggc gctgggcgct 1140

gatctcggta aatcggtaat ggcgatcgcc tacggcgagc aatggatgaa catggcacaa 1200

ccattctggg cgctgccagc actggcaatc gccggactcg gtgtccgcga catcatgggc 1260

tactgcatca ctgccctgct cttctccggt gtcattttcg tcattggttt aacgctgttc 1320

tga 1323

<210> 91

<211> 166

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 91

Met His Ser Gln Ile Trp Val Val Ser Thr Leu Leu Ile Ser Ile Val

1 5 10 15

Leu Ile Val Leu Thr Ile Val Lys Phe Lys Phe His Pro Phe Leu Ala

20 25 30

Leu Leu Leu Ala Ser Phe Phe Val Gly Thr Met Met Gly Met Gly Pro

35 40 45

Leu Asp Met Val Asn Ala Ile Glu Ser Gly Ile Gly Gly Thr Leu Gly

50 55 60

Phe Leu Ala Ala Val Ile Gly Leu Gly Thr Ile Leu Gly Lys Met Met

65 70 75 80

Glu Val Ser Gly Ala Ala Glu Arg Ile Gly Leu Thr Leu Gln Arg Cys

85 90 95

Arg Trp Leu Ser Val Asp Val Ile Met Val Leu Val Gly Leu Ile Cys

100 105 110

Gly Ile Thr Leu Phe Val Glu Val Gly Val Val Leu Leu Ile Pro Leu

115 120 125

Ala Phe Ser Ile Ala Lys Lys Thr Asn Thr Ser Leu Leu Lys Leu Ala

130 135 140

Ile Pro Leu Cys Thr Ala Leu Met Ala Val His Cys Val Val Pro Pro

145 150 155 160

His Pro Ala Ala Leu Tyr

165

<210> 92

<211> 1338

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 92

atgcactctc aaatctgggt tgtgagcacg ctgcttatca gcatcgtgtt aattgtactg 60

accatcgtga agttcaaatt ccacccgttt ctggcgctgt tgctggccag cttcttcgtg 120

ggaacgatga tgggcatggg gccactggat atggtaaatg ctattgaaag tggaattggc 180

ggaacgctgg ggttcctcgc agcggttatc ggccttggca cgatactggg aaaaatgatg 240

gaagtatccg gggccgcaga aagaattggt ctgacacttc aacgctgccg ctggctttca 300

gttgatgtca ttatggtgct ggttggcctg atttgtggca tcacgctgtt tgttgaagtg 360

ggcgtcgtgc tattgattcc tctggctttt tcaattgcca aaaaaaccaa tacctcatta 420

ttaaagcttg ccattccgct atgtaccgca ttgatggcag tgcactgcgt ggttcctcca 480

catccggctg ctttatatgt tgccaataag ctgggcgcag atatcggttc ggtgatcgtc 540

tacggtttgc tggttgggct gatggcatca ctgatcggtg gcccactttt ccttaaattt 600

ctgggtcaac gactgccctt taaacctgta cccacagagt ttgcagatct caaagttcgc 660

gatgaaaaaa cactaccgtc attaggcgca acgttattca ccatactgct acccattgcg 720

ctgatgttgg ttaaaacgat tgccgaattg aatatggcgc gtgagagtgg tttgtatatc 780

ttggttgagt ttattggcaa ccctatcact gccatgttta tcgccgtgtt tgtcgcctat 840

tatgtgttgg gtatacgcca gcatatgagc atggggacga tgctcacaca tacggaaaat 900

ggcttcggtt ctattgctaa tattttgctg attatcgggg ccggaggcgc attcaacgcc 960

attttaaaaa gcagcagtct cgctgatacg ctggcagtta ttctctccaa tatgcatatg 1020

cacccgattc ttctggcctg gttagtggct cttattctgc atgcggcagt gggctccgct 1080

accgtggcaa tgatgggggc aacggcaatt gttgcaccca tgctgccgct gtatcccgac 1140

atcagcccgg aaattattgc gattgctatc ggttcaggtg caattggctg cactatcgtt 1200

acggactcgc ttttctggct agtgaagcaa tattgcggcg ctacgctcaa tgaaacattt 1260

aaatactata cgacagcgac atttatcgct tcagtcgtcg ctctggcggg cacattcctg 1320

ctgtcattta tcatctaa 1338

<210> 93

<211> 151

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 93

Met Glu Ser Tyr Ser Gln Asn Ser Asn Lys Leu Asp Phe Gln His Glu

1 5 10 15

Ala Arg Ile Leu Asn Gly Ile Trp Leu Ile Thr Ala Leu Gly Leu Val

20 25 30

Ala Thr Ala Gly Leu Ala Trp Gly Ala Lys Tyr Ile Glu Ile Thr Ala

35 40 45

Thr Lys Tyr Asp Ser Pro Pro Met Tyr Val Ala Ile Gly Leu Leu Leu

50 55 60

Leu Cys Met Tyr Gly Leu Ser Lys Asp Ile Asn Lys Ile Asn Ala Ala

65 70 75 80

Ile Ala Gly Val Ile Tyr Leu Phe Leu Leu Ser Leu Val Ala Ile Val

85 90 95

Val Ala Ser Leu Val Pro Val Tyr Ala Ile Ile Ile Val Phe Ser Thr

100 105 110

Ala Gly Ala Met Phe Leu Ile Ser Met Leu Ala Gly Leu Leu Phe Asn

115 120 125

Val Asp Pro Gly Ser His Arg Phe Ile Ile Met Met Thr Leu Thr Gly

130 135 140

Leu Ala Leu Val Ile Ile Val

145 150

<210> 94

<211> 714

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 94

atggaatcat actcgcaaaa cagcaataaa ttagattttc agcacgaggc caggatatta 60

aacggtatat ggctcattac cgctttaggt ttggtggcaa ccgcaggact agcctgggga 120

gctaagtata tcgaaattac ggcaaccaaa tatgattcac caccaatgta tgtcgccata 180

gggttattat tactttgtat gtatggctta agtaaggata tcaacaagat aaatgccgcc 240

atcgcgggcg taatatatct gtttttactc tctttggtgg cgattgtcgt tgcaagttta 300

gttcctgtat atgccattat catcgtgttc agcactgcgg gcgcgatgtt tttaatcagt 360

atgctggccg gtttattatt taatgttgat cctggttctc accgttttat cattatgatg 420

acgttgacag ggttggccct ggtaatcatc gtgaatgcgg cattaatgag tgaacggccc 480

atttggataa taagttgctt aatgattgtg ttatggtcag gcattatctc gcatggacga 540

aataagctcc ttgaattggc ggggaaatgc catagtgaag agttgtggag tccggttcgt 600

tgcgctttta caggtgcatt aacactctat tactatttta tcggcttctt tgggatactt 660

gccgcgatag ctataacgct tgtctggcaa aggcatacgc gtttttttca ttag 714

<210> 95

<211> 369

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 95

Met Lys Ser Arg Ala Ala Val Ala Phe Ala Pro Gly Lys Pro Leu Glu

1 5 10 15

Ile Val Glu Ile Asp Val Ala Pro Pro Lys Lys Gly Glu Val Leu Ile

20 25 30

Lys Val Thr His Thr Gly Val Cys His Thr Asp Ala Phe Thr Leu Ser

35 40 45

Gly Asp Asp Pro Glu Gly Val Phe Pro Val Val Leu Gly His Glu Gly

50 55 60

Ala Gly Val Val Val Glu Val Gly Glu Gly Val Thr Ser Val Lys Pro

65 70 75 80

Gly Asp His Val Ile Pro Leu Tyr Thr Ala Glu Cys Gly Glu Cys Glu

85 90 95

Phe Cys Arg Ser Gly Lys Thr Asn Leu Cys Val Ala Val Arg Glu Thr

100 105 110

Gln Gly Lys Gly Leu Met Pro Asp Gly Thr Thr Arg Phe Ser Tyr Asn

115 120 125

Gly Gln Pro Leu Tyr His Tyr Met Gly Cys Ser Thr Phe Ser Glu Tyr

130 135 140

Thr Val Val Ala Glu Val Ser Leu Ala Lys Ile Asn Pro Glu Ala Asn

145 150 155 160

His Glu His Val Cys Leu Leu Gly Cys Gly Val Thr Thr Gly Ile Gly

165 170 175

Ala Val His Asn Thr Ala Lys Val Gln Pro Gly Asp Ser Val Ala Val

180 185 190

Phe Gly Leu Gly Ala Ile Gly Leu Ala Val Val Gln Gly Ala Arg Gln

195 200 205

Ala Lys Ala Gly Arg Ile Ile Ala Ile Asp Thr Asn Pro Lys Lys Phe

210 215 220

Asp Leu Ala Arg Arg Phe Gly Ala Thr Asp Cys Ile Asn Pro Asn Asp

225 230 235 240

Tyr Asp Lys Pro Ile Lys Asp Val Leu Leu Asp Ile Asn Lys Trp Gly

245 250 255

Ile Asp His Thr Phe Glu Cys Ile Gly Asn Val Asn Val Met Arg Ala

260 265 270

Ala Leu Glu Ser Ala His Arg Gly Trp Gly Gln Ser Val Ile Ile Gly

275 280 285

Val Ala Val Ala Gly Gln Glu Ile Ser Thr Arg Pro Phe Gln Leu Val

290 295 300

Thr Gly Arg Val Trp Lys Gly Ser Ala Phe Gly Gly Val Lys Gly Arg

305 310 315 320

Ser Gln Leu Pro Gly Met Val Glu Asp Ala Met Lys Gly Asp Ile Asp

325 330 335

Leu Glu Pro Phe Val Thr His Thr Met Ser Leu Asp Glu Ile Asn Asp

340 345 350

Ala Phe Asp Leu Met His Glu Gly Lys Ser Ile Arg Thr Val Ile Arg

355 360 365

Tyr

<210> 96

<211> 336

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 96

Met Lys Ala Ala Val Val Thr Lys Asp His His Val Asp Val Thr Tyr

1 5 10 15

Lys Thr Leu Arg Ser Leu Lys His Gly Glu Ala Leu Leu Lys Met Glu

20 25 30

Cys Cys Gly Val Cys His Thr Asp Leu His Val Lys Asn Gly Asp Phe

35 40 45

Gly Asp Lys Thr Gly Val Ile Leu Gly His Glu Gly Ile Gly Val Val

50 55 60

Ala Glu Val Gly Pro Gly Val Thr Ser Leu Lys Pro Gly Asp Arg Ala

65 70 75 80

Ser Val Ala Trp Phe Tyr Glu Gly Cys Gly His Cys Glu Tyr Cys Asn

85 90 95

Ser Gly Asn Glu Thr Leu Cys Arg Ser Val Lys Asn Ala Gly Tyr Ser

100 105 110

Val Asp Gly Gly Met Ala Glu Glu Cys Ile Val Val Ala Asp Tyr Ala

115 120 125

Val Lys Val Pro Asp Gly Leu Asp Ser Ala Ala Ala Ser Ser Ile Thr

130 135 140

Cys Ala Gly Val Thr Thr Tyr Lys Ala Val Lys Leu Ser Lys Ile Arg

145 150 155 160

Pro Gly Gln Trp Ile Ala Ile Tyr Gly Leu Gly Gly Leu Gly Asn Leu

165 170 175

Ala Leu Gln Tyr Ala Lys Asn Val Phe Asn Ala Lys Val Ile Ala Ile

180 185 190

Asp Val Asn Asp Glu Gln Leu Lys Leu Ala Thr Glu Met Gly Ala Asp

195 200 205

Leu Ala Ile Asn Ser His Thr Glu Asp Ala Ala Lys Ile Val Gln Glu

210 215 220

Lys Thr Gly Gly Ala His Ala Ala Val Val Thr Ala Val Ala Lys Ala

225 230 235 240

Ala Phe Asn Ser Ala Val Asp Ala Val Arg Ala Gly Gly Arg Val Val

245 250 255

Ala Val Gly Leu Pro Pro Glu Ser Met Ser Leu Asp Ile Pro Arg Leu

260 265 270

Val Leu Asp Gly Ile Glu Val Val Gly Ser Leu Val Gly Thr Arg Gln

275 280 285

Asp Leu Thr Glu Ala Phe Gln Phe Ala Ala Glu Gly Lys Val Val Pro

290 295 300

Lys Val Ala Leu Arg Pro Leu Ala Asp Ile Asn Thr Ile Phe Thr Glu

305 310 315 320

Met Glu Glu Gly Lys Ile Arg Gly Arg Met Val Ile Asp Phe Arg His

325 330 335

<210> 97

<211> 383

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 97

Met Ala Ala Ser Thr Phe Phe Ile Pro Ser Val Asn Val Ile Gly Ala

1 5 10 15

Asp Ser Leu Thr Asp Ala Met Asn Met Met Ala Asp Tyr Gly Phe Thr

20 25 30

Arg Thr Leu Ile Val Thr Asp Asn Met Leu Thr Lys Leu Gly Met Ala

35 40 45

Gly Asp Val Gln Lys Ala Leu Glu Glu Arg Asn Ile Phe Ser Val Ile

50 55 60

Tyr Asp Gly Thr Gln Pro Asn Pro Thr Thr Glu Asn Val Ala Ala Gly

65 70 75 80

Leu Lys Leu Leu Lys Glu Asn Asn Cys Asp Ser Val Ile Ser Leu Gly

85 90 95

Gly Gly Ser Pro His Asp Cys Ala Lys Gly Ile Ala Leu Val Ala Ala

100 105 110

Asn Gly Gly Asp Ile Arg Asp Tyr Glu Gly Val Asp Arg Ser Ala Lys

115 120 125

Pro Gln Leu Pro Met Ile Ala Ile Asn Thr Thr Ala Gly Thr Ala Ser

130 135 140

Glu Met Thr Arg Phe Cys Ile Ile Thr Asp Glu Ala Arg His Ile Lys

145 150 155 160

Met Ala Ile Val Asp Lys His Val Thr Pro Leu Leu Ser Val Asn Asp

165 170 175

Ser Ser Leu Met Ile Gly Met Pro Lys Ser Leu Thr Ala Ala Thr Gly

180 185 190

Met Asp Ala Leu Thr His Ala Ile Glu Ala Tyr Val Ser Ile Ala Ala

195 200 205

Thr Pro Ile Thr Asp Ala Cys Ala Leu Lys Ala Val Thr Met Ile Ala

210 215 220

Glu Asn Leu Pro Leu Ala Val Glu Asp Gly Ser Asn Ala Lys Ala Arg

225 230 235 240

Glu Ala Met Ala Tyr Ala Gln Phe Leu Ala Gly Met Ala Phe Asn Asn

245 250 255

Ala Ser Leu Gly Tyr Val His Ala Met Ala His Gln Leu Gly Gly Phe

260 265 270

Tyr Asn Leu Pro His Gly Val Cys Asn Ala Val Leu Leu Pro His Val

275 280 285

Gln Val Phe Asn Ser Lys Val Ala Ala Ala Arg Leu Arg Asp Cys Ala

290 295 300

Ala Ala Met Gly Val Asn Val Thr Gly Lys Asn Asp Ala Glu Gly Ala

305 310 315 320

Glu Ala Cys Ile Asn Ala Ile Arg Glu Leu Ala Lys Lys Val Asp Ile

325 330 335

Pro Ala Gly Leu Arg Asp Leu Asn Val Lys Glu Glu Asp Phe Ala Val

340 345 350

Leu Ala Thr Asn Ala Leu Lys Asp Ala Cys Gly Phe Thr Asn Pro Ile

355 360 365

Gln Ala Thr His Glu Glu Ile Val Ala Ile Tyr Arg Ala Ala Met

370 375 380

<210> 98

<211> 395

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 98

Met Gln Asn Glu Leu Gln Thr Ala Leu Phe Gln Ala Phe Asp Thr Leu

1 5 10 15

Asn Leu Gln Arg Val Lys Thr Phe Ser Val Pro Pro Val Thr Leu Cys

20 25 30

Gly Pro Gly Ser Val Ser Ser Cys Gly Gln Gln Ala Gln Thr Arg Gly

35 40 45

Leu Lys His Leu Phe Val Met Ala Asp Ser Phe Leu His Gln Ala Gly

50 55 60

Met Thr Ala Gly Leu Thr Arg Ser Leu Thr Val Lys Gly Ile Ala Met

65 70 75 80

Thr Leu Trp Pro Cys Pro Val Gly Glu Pro Cys Ile Thr Asp Val Cys

85 90 95

Ala Ala Val Ala Gln Leu Arg Glu Ser Gly Cys Asp Gly Val Ile Ala

100 105 110

Phe Gly Gly Gly Ser Val Leu Asp Ala Ala Lys Ala Val Thr Leu Leu

115 120 125

Val Thr Asn Pro Asp Ser Thr Leu Ala Glu Met Ser Glu Thr Ser Val

130 135 140

Leu Gln Pro Arg Leu Pro Leu Ile Ala Ile Pro Thr Thr Ala Gly Thr

145 150 155 160

Gly Ser Glu Thr Thr Asn Val Thr Val Ile Ile Asp Ala Val Ser Gly

165 170 175

Arg Lys Gln Val Leu Ala His Ala Ser Leu Met Pro Asp Val Ala Ile

180 185 190

Leu Asp Ala Ala Leu Thr Glu Gly Val Pro Ser His Val Thr Ala Met

195 200 205

Thr Gly Ile Asp Ala Leu Thr His Ala Ile Glu Ala Tyr Ser Ala Leu

210 215 220

Asn Ala Thr Pro Phe Thr Asp Ser Leu Ala Ile Gly Ala Ile Ala Met

225 230 235 240

Ile Gly Lys Ser Leu Pro Lys Ala Val Gly Tyr Gly His Asp Leu Ala

245 250 255

Ala Arg Glu Ser Met Leu Leu Ala Ser Cys Met Ala Gly Met Ala Phe

260 265 270

Ser Ser Ala Gly Leu Gly Leu Cys His Ala Met Ala His Gln Pro Gly

275 280 285

Ala Ala Leu His Ile Pro His Gly Leu Ala Asn Ala Met Leu Leu Pro

290 295 300

Thr Val Met Glu Phe Asn Arg Met Val Cys Arg Glu Arg Phe Ser Gln

305 310 315 320

Ile Gly Arg Ala Leu Arg Thr Lys Lys Ser Asp Asp Arg Asp Ala Ile

325 330 335

Asn Ala Val Ser Glu Leu Ile Ala Glu Val Gly Ile Gly Lys Arg Leu

340 345 350

Gly Asp Val Gly Ala Thr Ser Ala His Tyr Gly Ala Trp Ala Gln Ala

355 360 365

Ala Leu Glu Asp Ile Cys Leu Arg Ser Asn Pro Arg Thr Ala Ser Leu

370 375 380

Glu Gln Ile Val Gly Leu Tyr Ala Ala Ala Gln

385 390 395

<210> 99

<211> 387

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 99

Met Asn Asn Phe Asn Leu His Thr Pro Thr Arg Ile Leu Phe Gly Lys

1 5 10 15

Gly Ala Ile Ala Gly Leu Arg Glu Gln Ile Pro His Asp Ala Arg Val

20 25 30

Leu Ile Thr Tyr Gly Gly Gly Ser Val Lys Lys Thr Gly Val Leu Asp

35 40 45

Gln Val Leu Asp Ala Leu Lys Gly Met Asp Val Leu Glu Phe Gly Gly

50 55 60

Ile Glu Pro Asn Pro Ala Tyr Glu Thr Leu Met Asn Ala Val Lys Leu

65 70 75 80

Val Arg Glu Gln Lys Val Thr Phe Leu Leu Ala Val Gly Gly Gly Ser

85 90 95

Val Leu Asp Gly Thr Lys Phe Ile Ala Ala Ala Ala Asn Tyr Pro Glu

100 105 110

Asn Ile Asp Pro Trp His Ile Leu Gln Thr Gly Gly Lys Glu Ile Lys

115 120 125

Ser Ala Ile Pro Met Gly Cys Val Leu Thr Leu Pro Ala Thr Gly Ser

130 135 140

Glu Ser Asn Ala Gly Ala Val Ile Ser Arg Lys Thr Thr Gly Asp Lys

145 150 155 160

Gln Ala Phe His Ser Ala His Val Gln Pro Val Phe Ala Val Leu Asp

165 170 175

Pro Val Tyr Thr Tyr Thr Leu Pro Pro Arg Gln Val Ala Asn Gly Val

180 185 190

Val Asp Ala Phe Val His Thr Val Glu Gln Tyr Val Thr Lys Pro Val

195 200 205

Asp Ala Lys Ile Gln Asp Arg Phe Ala Glu Gly Ile Leu Leu Thr Leu

210 215 220

Ile Glu Asp Gly Pro Lys Ala Leu Lys Glu Pro Glu Asn Tyr Asp Val

225 230 235 240

Arg Ala Asn Val Met Trp Ala Ala Thr Gln Ala Leu Asn Gly Leu Ile

245 250 255

Gly Ala Gly Val Pro Gln Asp Trp Ala Thr His Met Leu Gly His Glu

260 265 270

Leu Thr Ala Met His Gly Leu Asp His Ala Gln Thr Leu Ala Ile Val

275 280 285

Leu Pro Ala Leu Trp Asn Glu Lys Arg Asp Thr Lys Arg Ala Lys Leu

290 295 300

Leu Gln Tyr Ala Glu Arg Val Trp Asn Ile Thr Glu Gly Ser Asp Asp

305 310 315 320

Glu Arg Ile Asp Ala Ala Ile Ala Ala Thr Arg Asn Phe Phe Glu Gln

325 330 335

Leu Gly Val Pro Thr His Leu Ser Asp Tyr Gly Leu Asp Gly Ser Ser

340 345 350

Ile Pro Ala Leu Leu Lys Lys Leu Glu Glu His Gly Met Thr Gln Leu

355 360 365

Gly Glu Asn His Asp Ile Thr Leu Asp Val Ser Arg Arg Ile Tyr Glu

370 375 380

Ala Ala Arg

385

<210> 100

<211> 284

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 100

Met Gln Gln Lys Met Ile Gln Phe Ser Gly Asp Val Ser Leu Pro Ala

1 5 10 15

Val Gly Gln Gly Thr Trp Tyr Met Gly Glu Asp Ala Ser Gln Arg Lys

20 25 30

Thr Glu Val Ala Ala Leu Arg Ala Gly Ile Glu Leu Gly Leu Thr Leu

35 40 45

Ile Asp Thr Ala Glu Met Tyr Ala Asp Gly Gly Ala Glu Lys Val Val

50 55 60

Gly Glu Ala Leu Thr Gly Leu Arg Glu Lys Val Phe Leu Val Ser Lys

65 70 75 80

Val Tyr Pro Trp Asn Ala Gly Gly Gln Lys Ala Ile Asn Ala Cys Glu

85 90 95

Ala Ser Leu Arg Arg Leu Asn Thr Asp Tyr Leu Asp Leu Tyr Leu Leu

100 105 110

His Trp Ser Gly Ser Phe Ala Phe Glu Glu Thr Val Ala Ala Met Glu

115 120 125

Lys Leu Ile Ala Gln Gly Lys Ile Arg Arg Trp Gly Val Ser Asn Leu

130 135 140

Asp Tyr Ala Asp Met Gln Glu Leu Trp Gln Leu Pro Gly Gly Asn Gln

145 150 155 160

Cys Ala Thr Asn Gln Val Leu Tyr His Leu Gly Ser Arg Gly Ile Glu

165 170 175

Tyr Asp Leu Leu Pro Trp Cys Gln Gln Gln Gln Met Pro Val Met Ala

180 185 190

Tyr Ser Pro Leu Ala Gln Ala Gly Arg Leu Arg Asn Gly Leu Leu Lys

195 200 205

Asn Ala Val Val Asn Glu Ile Ala His Ala His Asn Ile Ser Ala Ala

210 215 220

Gln Val Leu Leu Ala Trp Val Ile Ser His Gln Gly Val Met Ala Ile

225 230 235 240

Pro Lys Ala Ala Thr Ile Ala His Val Gln Gln Asn Ala Ala Val Leu

245 250 255

Glu Val Glu Leu Ser Ser Ala Glu Leu Ala Met Leu Asp Lys Ala Tyr

260 265 270

Pro Ala Pro Lys Gly Lys Thr Ala Leu Asp Met Val

275 280

<210> 101

<211> 298

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 101

Met Val Gln Arg Ile Thr Ile Ala Pro Gln Gly Pro Glu Phe Ser Arg

1 5 10 15

Phe Val Met Gly Tyr Trp Arg Leu Met Asp Trp Asn Met Ser Ala Arg

20 25 30

Gln Leu Val Ser Phe Ile Glu Glu His Leu Asp Leu Gly Val Thr Thr

35 40 45

Val Asp His Ala Asp Ile Tyr Gly Gly Tyr Gln Cys Glu Ala Ala Phe

50 55 60

Gly Glu Ala Leu Lys Leu Ala Pro His Leu Arg Glu Arg Met Glu Ile

65 70 75 80

Val Ser Lys Cys Gly Ile Ala Thr Thr Ala Arg Glu Glu Asn Val Ile

85 90 95

Gly His Tyr Ile Thr Asp Arg Asp His Ile Ile Lys Ser Ala Glu Gln

100 105 110

Ser Leu Ile Asn Leu Ala Thr Asp His Leu Asp Leu Leu Leu Ile His

115 120 125

Arg Pro Asp Pro Leu Met Asp Ala Asp Glu Val Ala Asp Ala Phe Lys

130 135 140

His Leu His Gln Ser Gly Lys Val Arg His Phe Gly Val Ser Asn Phe

145 150 155 160

Thr Pro Ala Gln Phe Ala Leu Leu Gln Ser Arg Leu Pro Phe Thr Leu

165 170 175

Ala Thr Asn Gln Val Glu Ile Ser Pro Val His Gln Pro Leu Leu Leu

180 185 190

Asp Gly Thr Leu Asp Gln Leu Gln Gln Leu Arg Val Arg Pro Met Ala

195 200 205

Trp Ser Cys Leu Gly Gly Gly Arg Leu Phe Asn Asp Asp Tyr Phe Gln

210 215 220

Pro Leu Arg Asp Glu Leu Ala Val Val Ala Glu Glu Leu Asn Ala Gly

225 230 235 240

Ser Ile Glu Gln Val Val Tyr Ala Trp Val Leu Arg Leu Pro Ser Gln

245 250 255

Pro Leu Pro Ile Ile Gly Ser Gly Lys Ile Glu Arg Val Arg Ala Ala

260 265 270

Val Glu Ala Glu Thr Leu Lys Met Thr Arg Gln Gln Trp Phe Arg Ile

275 280 285

Arg Lys Ala Ala Leu Gly Tyr Asp Val Pro

290 295

<210> 102

<211> 122

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 102

Met Trp Leu Leu Asp Gln Trp Ala Glu Arg His Ile Ala Glu Ala Gln

1 5 10 15

Ala Lys Gly Glu Phe Asp Asn Leu Ala Gly Ser Gly Glu Pro Leu Ile

20 25 30

Leu Asp Asp Asp Ser His Val Pro Pro Glu Leu Arg Ala Gly Tyr Arg

35 40 45

Leu Leu Lys Asn Ala Gly Cys Leu Pro Pro Glu Leu Glu Gln Arg Arg

50 55 60

Glu Ala Ile Gln Leu Leu Asp Ile Leu Lys Gly Ile Arg His Asp Asp

65 70 75 80

Pro Gln Tyr Gln Glu Val Ser Arg Arg Leu Ser Leu Leu Glu Leu Lys

85 90 95

Leu Arg Gln Ala Gly Leu Ser Thr Asp Phe Leu Arg Gly Asp Tyr Ala

100 105 110

Asp Lys Leu Leu Asp Lys Ile Asn Asp Asn

115 120

<210> 103

<211> 412

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 103

Met Lys Ala Leu Thr Tyr His Gly Pro His His Val Gln Val Glu Asn

1 5 10 15

Val Pro Asp Pro Gly Val Glu Gln Ala Asp Asp Ile Ile Leu Arg Ile

20 25 30

Thr Ala Thr Ala Ile Cys Gly Ser Asp Leu His Leu Tyr Arg Gly Lys

35 40 45

Ile Pro Gln Val Lys His Gly Asp Ile Phe Gly His Glu Phe Met Gly

50 55 60

Glu Val Val Glu Thr Gly Lys Asp Val Lys Asn Leu Gln Lys Gly Asp

65 70 75 80

Arg Val Val Ile Pro Phe Val Ile Ala Cys Gly Asp Cys Phe Phe Cys

85 90 95

Arg Leu Gln Gln Tyr Ala Ala Cys Glu Asn Thr Asn Ala Gly Lys Gly

100 105 110

Ala Ala Leu Asn Lys Lys Gln Ile Pro Ala Pro Ala Ala Leu Phe Gly

115 120 125

Tyr Ser His Leu Tyr Gly Gly Val Pro Gly Gly Gln Ala Glu Tyr Val

130 135 140

Arg Val Pro Lys Gly Asn Val Gly Pro Phe Lys Val Pro Pro Leu Leu

145 150 155 160

Ser Asp Asp Lys Ala Leu Phe Leu Ser Asp Ile Leu Pro Thr Ala Trp

165 170 175

Gln Ala Ala Lys Asn Ala Gln Ile Gln Gln Gly Ser Ser Val Ala Val

180 185 190

Tyr Gly Ala Gly Pro Val Gly Leu Leu Thr Ile Ala Cys Ala Arg Leu

195 200 205

Leu Gly Ala Glu Gln Ile Phe Val Val Asp His His Pro Tyr Arg Leu

210 215 220

His Phe Ala Ala Asp Arg Tyr Gly Ala Ile Pro Ile Asn Phe Asp Glu

225 230 235 240

Asp Ser Asp Pro Ala Gln Ser Ile Ile Glu Gln Thr Ala Gly His Arg

245 250 255

Gly Val Asp Ala Val Ile Asp Ala Val Gly Phe Glu Ala Lys Gly Ser

260 265 270

Thr Thr Glu Thr Val Leu Thr Asn Leu Lys Leu Glu Gly Ser Ser Gly

275 280 285

Lys Ala Leu Arg Gln Cys Ile Ala Ala Val Arg Arg Gly Gly Ile Val

290 295 300

Ser Val Pro Gly Val Tyr Ala Gly Phe Ile His Gly Phe Leu Phe Gly

305 310 315 320

Asp Ala Phe Asp Lys Gly Leu Ser Phe Lys Met Gly Gln Thr His Val

325 330 335

His Ala Trp Leu Gly Glu Leu Leu Pro Leu Ile Glu Lys Gly Leu Leu

340 345 350

Lys Pro Glu Glu Ile Val Thr His Tyr Met Pro Phe Glu Glu Ala Ala

355 360 365

Arg Gly Tyr Glu Ile Phe Glu Lys Arg Glu Glu Glu Cys Arg Lys Val

370 375 380

Ile Leu Val Pro Gly Ala Gln Ser Ala Glu Ala Ala Gln Lys Ala Val

385 390 395 400

Ser Gly Leu Val Asn Ala Met Pro Gly Gly Thr Ile

405 410

<210> 104

<211> 362

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 104

Met Pro His Asn Pro Ile Arg Val Val Val Gly Pro Ala Asn Tyr Phe

1 5 10 15

Ser His Pro Gly Ser Phe Asn His Leu His Asp Phe Phe Thr Asp Glu

20 25 30

Gln Leu Ser Arg Ala Val Trp Ile Tyr Gly Lys Arg Ala Ile Ala Ala

35 40 45

Ala Gln Thr Lys Leu Pro Pro Ala Phe Gly Leu Pro Gly Ala Lys His

50 55 60

Ile Leu Phe Arg Gly His Cys Ser Glu Ser Asp Val Gln Gln Leu Ala

65 70 75 80

Ala Glu Ser Gly Asp Asp Arg Ser Val Val Ile Gly Val Gly Gly Gly

85 90 95

Ala Leu Leu Asp Thr Ala Lys Ala Leu Ala Arg Arg Leu Gly Leu Pro

100 105 110

Phe Val Ala Val Pro Thr Ile Ala Ala Thr Cys Ala Ala Trp Thr Pro

115 120 125

Leu Ser Val Trp Tyr Asn Asp Ala Gly Gln Ala Leu His Tyr Glu Ile

130 135 140

Phe Asp Asp Ala Asn Phe Met Val Leu Val Glu Pro Glu Ile Ile Leu

145 150 155 160

Asn Ala Pro Gln Gln Tyr Leu Leu Ala Gly Ile Gly Asp Thr Leu Ala

165 170 175

Lys Trp Tyr Glu Ala Val Val Leu Ala Pro Gln Pro Glu Thr Leu Pro

180 185 190

Leu Thr Val Arg Leu Gly Ile Asn Asn Ala Gln Ala Ile Arg Asp Val

195 200 205

Leu Leu Asn Ser Ser Glu Gln Ala Leu Ser Asp Gln Gln Asn Gln Gln

210 215 220

Leu Thr Gln Ser Phe Cys Asp Val Val Asp Ala Ile Ile Ala Gly Gly

225 230 235 240

Gly Met Val Gly Gly Leu Gly Asp Arg Phe Thr Arg Val Ala Ala Ala

245 250 255

His Ala Val His Asn Gly Leu Thr Val Leu Pro Gln Thr Glu Lys Phe

260 265 270

Leu His Gly Thr Lys Val Ala Tyr Gly Ile Leu Val Gln Ser Ala Leu

275 280 285

Leu Gly Gln Asp Asp Val Leu Ala Gln Leu Thr Gly Ala Tyr Gln Arg

290 295 300

Phe His Leu Pro Thr Thr Leu Ala Glu Leu Glu Val Asp Ile Asn Asn

305 310 315 320

Gln Ala Glu Ile Asp Lys Val Ile Ala His Thr Leu Arg Pro Val Glu

325 330 335

Ser Ile His Tyr Leu Pro Val Thr Leu Thr Pro Asp Thr Leu Arg Ala

340 345 350

Ala Phe Lys Lys Val Glu Ser Phe Lys Ala

355 360

<210> 105

<211> 347

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 105

Met Lys Asn Ser Lys Ala Ile Leu Gln Val Pro Gly Thr Met Lys Ile

1 5 10 15

Ile Ser Ala Glu Ile Pro Val Pro Lys Glu Asp Glu Val Leu Ile Lys

20 25 30

Val Glu Tyr Val Gly Ile Cys Gly Ser Asp Val His Gly Phe Glu Ser

35 40 45

Gly Pro Phe Ile Pro Pro Lys Asp Pro Asn Gln Glu Ile Gly Leu Gly

50 55 60

His Glu Cys Ala Gly Thr Val Val Ala Val Gly Ser Arg Val Arg Lys

65 70 75 80

Phe Lys Pro Gly Asp Arg Val Asn Ile Glu Pro Gly Val Pro Cys Gly

85 90 95

His Cys Arg Tyr Cys Leu Glu Gly Lys Tyr Asn Ile Cys Pro Asp Val

100 105 110

Asp Phe Met Ala Thr Gln Pro Asn Tyr Arg Gly Ala Leu Thr His Tyr

115 120 125

Leu Cys His Pro Glu Ser Phe Thr Tyr Lys Leu Pro Asp Asn Met Asp

130 135 140

Thr Met Glu Gly Ala Leu Val Glu Pro Ala Ala Val Gly Met His Ala

145 150 155 160

Ala Met Leu Ala Asp Val Lys Pro Gly Lys Lys Ile Ile Ile Leu Gly

165 170 175

Ala Gly Cys Ile Gly Leu Met Thr Leu Gln Ala Cys Lys Cys Leu Gly

180 185 190

Ala Thr Glu Ile Ala Val Val Asp Val Leu Glu Lys Arg Leu Ala Met

195 200 205

Ala Glu Gln Leu Gly Ala Thr Val Val Ile Asn Gly Ala Lys Glu Asp

210 215 220

Thr Ile Ala Arg Cys Gln Gln Phe Thr Glu Asp Met Gly Ala Asp Ile

225 230 235 240

Val Phe Glu Thr Ala Gly Ser Ala Val Thr Val Lys Gln Ala Pro Tyr

245 250 255

Leu Val Met Arg Gly Gly Lys Ile Met Ile Val Gly Thr Val Pro Gly

260 265 270

Asp Ser Ala Ile Asn Phe Leu Lys Ile Asn Arg Glu Val Thr Ile Gln

275 280 285

Thr Val Phe Arg Tyr Ala Asn Arg Tyr Pro Val Thr Ile Glu Ala Ile

290 295 300

Ser Ser Gly Arg Phe Asp Val Lys Ser Met Val Thr His Ile Tyr Asp

305 310 315 320

Tyr Arg Asp Val Gln Gln Ala Phe Glu Glu Ser Val Asn Asn Lys Arg

325 330 335

Asp Ile Ile Lys Gly Val Ile Lys Ile Ser Asp

340 345

<210> 106

<211> 358

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 106

Met Lys Ala Leu Ala Arg Phe Gly Lys Ala Phe Gly Gly Tyr Lys Met

1 5 10 15

Ile Asp Val Pro Gln Pro Met Cys Gly Pro Glu Asp Val Val Ile Glu

20 25 30

Ile Lys Ala Ala Ala Ile Cys Gly Ala Asp Met Lys His Tyr Asn Val

35 40 45

Asp Ser Gly Ser Asp Glu Phe Asn Ser Ile Arg Gly His Glu Phe Ala

50 55 60

Gly Cys Ile Ala Gln Val Gly Glu Lys Val Lys Asp Trp Lys Val Gly

65 70 75 80

Gln Arg Val Val Ser Asp Asn Ser Gly His Val Cys Gly Val Cys Pro

85 90 95

Ala Cys Glu Gln Gly Asp Phe Leu Cys Cys Thr Glu Lys Val Asn Leu

100 105 110

Gly Leu Asp Asn Asn Thr Trp Gly Gly Gly Phe Ser Lys Tyr Cys Leu

115 120 125

Val Pro Gly Glu Ile Leu Lys Ile His Arg His Ala Leu Trp Glu Ile

130 135 140

Pro Asp Gly Val Asp Tyr Glu Asp Ala Ala Val Leu Asp Pro Ile Cys

145 150 155 160

Asn Ala Tyr Lys Ser Ile Ala Gln Gln Ser Lys Phe Leu Pro Gly Gln

165 170 175

Asp Val Val Val Ile Gly Thr Gly Pro Leu Gly Leu Phe Ser Val Gln

180 185 190

Met Ala Arg Ile Met Gly Ala Val Asn Ile Val Val Val Gly Leu Gln

195 200 205

Glu Asp Val Ala Val Arg Phe Pro Val Ala Lys Glu Leu Gly Ala Thr

210 215 220

Ala Val Val Asn Gly Ser Thr Glu Asp Val Val Ala Arg Cys Gln Gln

225 230 235 240

Ile Cys Gly Lys Asp Asn Leu Gly Leu Val Ile Glu Cys Ser Gly Ala

245 250 255

Asn Ile Ala Leu Lys Gln Ala Ile Glu Met Leu Arg Pro Asn Gly Glu

260 265 270

Val Val Arg Val Gly Met Gly Phe Lys Pro Leu Asp Phe Ser Ile Asn

275 280 285

Asp Ile Thr Ala Trp Asn Lys Ser Ile Ile Gly His Met Ala Tyr Asp

290 295 300

Ser Thr Ser Trp Arg Asn Ala Ile Arg Leu Leu Ala Ser Gly Ala Ile

305 310 315 320

Lys Val Lys Pro Met Ile Thr His Arg Ile Gly Leu Ser Gln Trp Arg

325 330 335

Glu Gly Phe Asp Ala Met Val Asp Lys Thr Ala Ile Lys Val Ile Met

340 345 350

Thr Tyr Asp Phe Asp Glu

355

<210> 107

<211> 390

<212> PRT

<213> 丙酮丁醇梭菌(Clostridium acetobutylicum)

<400> 107

Met Val Asp Phe Glu Tyr Ser Ile Pro Thr Arg Ile Phe Phe Gly Lys

1 5 10 15

Asp Lys Ile Asn Val Leu Gly Arg Glu Leu Lys Lys Tyr Gly Ser Lys

20 25 30

Val Leu Ile Val Tyr Gly Gly Gly Ser Ile Lys Arg Asn Gly Ile Tyr

35 40 45

Asp Lys Ala Val Ser Ile Leu Glu Lys Asn Ser Ile Lys Phe Tyr Glu

50 55 60

Leu Ala Gly Val Glu Pro Asn Pro Arg Val Thr Thr Val Glu Lys Gly

65 70 75 80

Val Lys Ile Cys Arg Glu Asn Gly Val Glu Val Val Leu Ala Ile Gly

85 90 95

Gly Gly Ser Ala Ile Asp Cys Ala Lys Val Ile Ala Ala Ala Cys Glu

100 105 110

Tyr Asp Gly Asn Pro Trp Asp Ile Val Leu Asp Gly Ser Lys Ile Lys

115 120 125

Arg Val Leu Pro Ile Ala Ser Ile Leu Thr Ile Ala Ala Thr Gly Ser

130 135 140

Glu Met Asp Thr Trp Ala Val Ile Asn Asn Met Asp Thr Asn Glu Lys

145 150 155 160

Leu Ile Ala Ala His Pro Asp Met Ala Pro Lys Phe Ser Ile Leu Asp

165 170 175

Pro Thr Tyr Thr Tyr Thr Val Pro Thr Asn Gln Thr Ala Ala Gly Thr

180 185 190

Ala Asp Ile Met Ser His Ile Phe Glu Val Tyr Phe Ser Asn Thr Lys

195 200 205

Thr Ala Tyr Leu Gln Asp Arg Met Ala Glu Ala Leu Leu Arg Thr Cys

210 215 220

Ile Lys Tyr Gly Gly Ile Ala Leu Glu Lys Pro Asp Asp Tyr Glu Ala

225 230 235 240

Arg Ala Asn Leu Met Trp Ala Ser Ser Leu Ala Ile Asn Gly Leu Leu

245 250 255

Thr Tyr Gly Lys Asp Thr Asn Trp Ser Val His Leu Met Glu His Glu

260 265 270

Leu Ser Ala Tyr Tyr Asp Ile Thr His Gly Val Gly Leu Ala Ile Leu

275 280 285

Thr Pro Asn Trp Met Glu Tyr Ile Leu Asn Asn Asp Thr Val Tyr Lys

290 295 300

Phe Val Glu Tyr Gly Val Asn Val Trp Gly Ile Asp Lys Glu Lys Asn

305 310 315 320

His Tyr Asp Ile Ala His Gln Ala Ile Gln Lys Thr Arg Asp Tyr Phe

325 330 335

Val Asn Val Leu Gly Leu Pro Ser Arg Leu Arg Asp Val Gly Ile Glu

340 345 350

Glu Glu Lys Leu Asp Ile Met Ala Lys Glu Ser Val Lys Leu Thr Gly

355 360 365

Gly Thr Ile Gly Asn Leu Arg Pro Val Asn Ala Ser Glu Val Leu Gln

370 375 380

Ile Phe Lys Lys Ser Val

385 390

<210> 108

<211> 389

<212> PRT

<213> 丙酮丁醇梭菌(Clostridium acetobutylicum)

<400> 108

Met Leu Ser Phe Asp Tyr Ser Ile Pro Thr Lys Val Phe Phe Gly Lys

1 5 10 15

Gly Lys Ile Asp Val Ile Gly Glu Glu Ile Lys Lys Tyr Gly Ser Arg

20 25 30

Val Leu Ile Val Tyr Gly Gly Gly Ser Ile Lys Arg Asn Gly Ile Tyr

35 40 45

Asp Arg Ala Thr Ala Ile Leu Lys Glu Asn Asn Ile Ala Phe Tyr Glu

50 55 60

Leu Ser Gly Val Glu Pro Asn Pro Arg Ile Thr Thr Val Lys Lys Gly

65 70 75 80

Ile Glu Ile Cys Arg Glu Asn Asn Val Asp Leu Val Leu Ala Ile Gly

85 90 95

Gly Gly Ser Ala Ile Asp Cys Ser Lys Val Ile Ala Ala Gly Val Tyr

100 105 110

Tyr Asp Gly Asp Thr Trp Asp Met Val Lys Asp Pro Ser Lys Ile Thr

115 120 125

Lys Val Leu Pro Ile Ala Ser Ile Leu Thr Leu Ser Ala Thr Gly Ser

130 135 140

Glu Met Asp Gln Ile Ala Val Ile Ser Asn Met Glu Thr Asn Glu Lys

145 150 155 160

Leu Gly Val Gly His Asp Asp Met Arg Pro Lys Phe Ser Val Leu Asp

165 170 175

Pro Thr Tyr Thr Phe Thr Val Pro Lys Asn Gln Thr Ala Ala Gly Thr

180 185 190

Ala Asp Ile Met Ser His Thr Phe Glu Ser Tyr Phe Ser Gly Val Glu

195 200 205

Gly Ala Tyr Val Gln Asp Gly Ile Ala Glu Ala Ile Leu Arg Thr Cys

210 215 220

Ile Lys Tyr Gly Lys Ile Ala Met Glu Lys Thr Asp Asp Tyr Glu Ala

225 230 235 240

Arg Ala Asn Leu Met Trp Ala Ser Ser Leu Ala Ile Asn Gly Leu Leu

245 250 255

Ser Leu Gly Lys Asp Arg Lys Trp Ser Cys His Pro Met Glu His Glu

260 265 270

Leu Ser Ala Tyr Tyr Asp Ile Thr His Gly Val Gly Leu Ala Ile Leu

275 280 285

Thr Pro Asn Trp Met Glu Tyr Ile Leu Asn Asp Asp Thr Leu His Lys

290 295 300

Phe Val Ser Tyr Gly Ile Asn Val Trp Gly Ile Asp Lys Asn Lys Asp

305 310 315 320

Asn Tyr Glu Ile Ala Arg Glu Ala Ile Lys Asn Thr Arg Glu Tyr Phe

325 330 335

Asn Ser Leu Gly Ile Pro Ser Lys Leu Arg Glu Val Gly Ile Gly Lys

340 345 350

Asp Lys Leu Glu Leu Met Ala Lys Gln Ala Val Arg Asn Ser Gly Gly

355 360 365

Thr Ile Gly Ser Leu Arg Pro Ile Asn Ala Glu Asp Val Leu Glu Ile

370 375 380

Phe Lys Lys Ser Tyr

385

<210> 109

<211> 386

<212> PRT

<213> 丙酮丁醇梭菌(Clostridium acetobutylicum)

<400> 109

Met Tyr Asn Phe Asp Phe Phe Asn Pro Thr His Ile Val Phe Gly Lys

1 5 10 15

Asp Arg Leu Asn Glu Leu Asp Asn Leu Val Pro Arg Asp Ala Lys Val

20 25 30

Leu Val Leu Tyr Gly Gly Gly Ser Val Lys Lys Phe Gly Thr Leu Glu

35 40 45

Lys Val Ile Asn Gly Leu Gly Asn Arg Gln Val Ile Glu Phe Gly Gly

50 55 60

Ile Glu Pro Asn Pro Gln Phe Thr Thr Leu Met Lys Ala Val Asp Ile

65 70 75 80

Val Lys Lys Glu Asn Ile Asp Phe Leu Leu Ala Val Gly Gly Gly Ser

85 90 95

Val Met Asp Gly Thr Lys Phe Val Ala Leu Ala Ala Tyr Tyr Glu Gly

100 105 110

Asp Asn Ala Ala Glu Ile Leu Tyr Ser Arg Glu Lys Ala Ala Ala Ile

115 120 125

Asn Lys Ala Val Pro Leu Gly Thr Val Val Thr Leu Pro Ala Thr Gly

130 135 140

Ser Glu Met Asn Asn Gly Gly Val Ile Ser Tyr Glu His Gly Lys Tyr

145 150 155 160

Gly Phe Gly Ser Lys Leu Val Phe Pro Lys Phe Ser Val Leu Asp Pro

165 170 175

Thr Leu Thr Tyr Thr Leu Pro Glu Ser Gln Val Ala Asn Gly Val Ala

180 185 190

Asp Thr Phe Val His Val Leu Glu Gln Tyr Ala Thr Phe Lys Ala Glu

195 200 205

Gly Arg Phe Gln Asp Arg Thr Ala Glu Gly Ile Leu Gln Thr Leu Ile

210 215 220

Glu Ile Gly Arg Lys Thr Ile Asp Asn Pro Thr Asp Tyr Asp Thr Arg

225 230 235 240

Ala Asn Leu Val Trp Cys Ala Thr Met Ala Leu Asn Gly Leu Ile Gly

245 250 255

Ala Gly Val Pro Gln Asp Trp Ser Thr His Met Ile Gly His Glu Leu

260 265 270

Thr Ala Met Phe Gly Ile Asp His Gly Lys Thr Leu Ala Ile Ile Leu

275 280 285

Pro Ser Ile Trp Asn Val Met Arg Glu Gln Lys Lys Gly Lys Ile Leu

290 295 300

Gln Tyr Ala Glu Arg Val Leu Gly Ile Thr Glu Gly Asp Asp Asp Ser

305 310 315 320

Arg Ile Asp Leu Ala Ile Leu Arg Thr Arg Glu Phe Phe Glu Ser Leu

325 330 335

Gly Ile Lys Thr His Leu Ser Glu Tyr Gly Val Thr Ala Asp Lys Ile

340 345 350

Asp Asp Ile Val Asn Ala Leu Asp Lys His Gly Met Lys Ala Leu Ser

355 360 365

Glu Thr Gly Ala Ile Thr Leu Glu Val Ser Arg Lys Ile Leu Glu Gly

370 375 380

Ala Met

385

<210> 110

<211> 348

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 110

Met Ser Ile Pro Glu Thr Gln Lys Gly Val Ile Phe Tyr Glu Ser His

1 5 10 15

Gly Lys Leu Glu Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Ala Asn

20 25 30

Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu

35 40 45

His Ala Trp His Gly Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val

50 55 60

Gly Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val

65 70 75 80

Lys Gly Trp Lys Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly

85 90 95

Ser Cys Met Ala Cys Glu Tyr Cys Glu Leu Gly Asn Glu Ser Asn Cys

100 105 110

Pro His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln

115 120 125

Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro Gln Gly Thr

130 135 140

Asp Leu Ala Gln Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr

145 150 155 160

Lys Ala Leu Lys Ser Ala Asn Leu Met Ala Gly His Trp Val Ala Ile

165 170 175

Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys

180 185 190

Ala Met Gly Tyr Arg Val Leu Gly Ile Asp Gly Gly Glu Gly Lys Glu

195 200 205

Glu Leu Phe Arg Ser Ile Gly Gly Glu Val Phe Ile Asp Phe Thr Lys

210 215 220

Glu Lys Asp Ile Val Gly Ala Val Leu Lys Ala Thr Asp Gly Gly Ala

225 230 235 240

His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile Glu Ala Ser

245 250 255

Thr Arg Tyr Val Arg Ala Asn Gly Thr Thr Val Leu Val Gly Met Pro

260 265 270

Ala Gly Ala Lys Cys Cys Ser Asp Val Phe Asn Gln Val Val Lys Ser

275 280 285

Ile Ser Ile Val Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu

290 295 300

Ala Leu Asp Phe Phe Ala Arg Gly Leu Val Lys Ser Pro Ile Lys Val

305 310 315 320

Val Gly Leu Ser Thr Leu Pro Glu Ile Tyr Glu Lys Met Glu Lys Gly

325 330 335

Gln Ile Val Gly Arg Tyr Val Val Asp Thr Ser Lys

340 345

<210> 111

<211> 348

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 111

Met Ser Ile Pro Glu Thr Gln Lys Ala Ile Ile Phe Tyr Glu Ser Asn

1 5 10 15

Gly Lys Leu Glu His Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn

20 25 30

Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu

35 40 45

His Ala Trp His Gly Asp Trp Pro Leu Pro Thr Lys Leu Pro Leu Val

50 55 60

Gly Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val

65 70 75 80

Lys Gly Trp Lys Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly

85 90 95

Ser Cys Met Ala Cys Glu Tyr Cys Glu Leu Gly Asn Glu Ser Asn Cys

100 105 110

Pro His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Glu

115 120 125

Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro Gln Gly Thr

130 135 140

Asp Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr

145 150 155 160

Lys Ala Leu Lys Ser Ala Asn Leu Arg Ala Gly His Trp Ala Ala Ile

165 170 175

Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys

180 185 190

Ala Met Gly Tyr Arg Val Leu Gly Ile Asp Gly Gly Pro Gly Lys Glu

195 200 205

Glu Leu Phe Thr Ser Leu Gly Gly Glu Val Phe Ile Asp Phe Thr Lys

210 215 220

Glu Lys Asp Ile Val Ser Ala Val Val Lys Ala Thr Asn Gly Gly Ala

225 230 235 240

His Gly Ile Ile Asn Val Ser Val Ser Glu Ala Ala Ile Glu Ala Ser

245 250 255

Thr Arg Tyr Cys Arg Ala Asn Gly Thr Val Val Leu Val Gly Leu Pro

260 265 270

Ala Gly Ala Lys Cys Ser Ser Asp Val Phe Asn His Val Val Lys Ser

275 280 285

Ile Ser Ile Val Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu

290 295 300

Ala Leu Asp Phe Phe Ala Arg Gly Leu Val Lys Ser Pro Ile Lys Val

305 310 315 320

Val Gly Leu Ser Ser Leu Pro Glu Ile Tyr Glu Lys Met Glu Lys Gly

325 330 335

Gln Ile Ala Gly Arg Tyr Val Val Asp Thr Ser Lys

340 345

<210> 112

<211> 375

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 112

Met Leu Arg Thr Ser Thr Leu Phe Thr Arg Arg Val Gln Pro Ser Leu

1 5 10 15

Phe Ser Arg Asn Ile Leu Arg Leu Gln Ser Thr Ala Ala Ile Pro Lys

20 25 30

Thr Gln Lys Gly Val Ile Phe Tyr Glu Asn Lys Gly Lys Leu His Tyr

35 40 45

Lys Asp Ile Pro Val Pro Glu Pro Lys Pro Asn Glu Ile Leu Ile Asn

50 55 60

Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His Ala Trp His Gly

65 70 75 80

Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val Gly Gly His Glu Gly

85 90 95

Ala Gly Val Val Val Lys Leu Gly Ser Asn Val Lys Gly Trp Lys Val

100 105 110

Gly Asp Leu Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Met Thr Cys

115 120 125

Glu Phe Cys Glu Ser Gly His Glu Ser Asn Cys Pro Asp Ala Asp Leu

130 135 140

Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Phe Ala Thr Ala Asp

145 150 155 160

Ala Ile Gln Ala Ala Lys Ile Gln Gln Gly Thr Asp Leu Ala Glu Val

165 170 175

Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys Ala Leu Lys Glu

180 185 190

Ala Asp Leu Lys Ala Gly Asp Trp Val Ala Ile Ser Gly Ala Ala Gly

195 200 205

Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Thr Ala Met Gly Tyr Arg

210 215 220

Val Leu Gly Ile Asp Ala Gly Glu Glu Lys Glu Lys Leu Phe Lys Lys

225 230 235 240

Leu Gly Gly Glu Val Phe Ile Asp Phe Thr Lys Thr Lys Asn Met Val

245 250 255

Ser Asp Ile Gln Glu Ala Thr Lys Gly Gly Pro His Gly Val Ile Asn

260 265 270

Val Ser Val Ser Glu Ala Ala Ile Ser Leu Ser Thr Glu Tyr Val Arg

275 280 285

Pro Cys Gly Thr Val Val Leu Val Gly Leu Pro Ala Asn Ala Tyr Val

290 295 300

Lys Ser Glu Val Phe Ser His Val Val Lys Ser Ile Asn Ile Lys Gly

305 310 315 320

Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Asp Phe Phe

325 330 335

Ser Arg Gly Leu Ile Lys Ser Pro Ile Lys Ile Val Gly Leu Ser Glu

340 345 350

Leu Pro Lys Val Tyr Asp Leu Met Glu Lys Gly Lys Ile Leu Gly Arg

355 360 365

Tyr Val Val Asp Thr Ser Lys

370 375

<210> 113

<211> 382

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 113

Met Ser Ser Val Thr Gly Phe Tyr Ile Pro Pro Ile Ser Phe Phe Gly

1 5 10 15

Glu Gly Ala Leu Glu Glu Thr Ala Asp Tyr Ile Lys Asn Lys Asp Tyr

20 25 30

Lys Lys Ala Leu Ile Val Thr Asp Pro Gly Ile Ala Ala Ile Gly Leu

35 40 45

Ser Gly Arg Val Gln Lys Met Leu Glu Glu Arg Asp Leu Asn Val Ala

50 55 60

Ile Tyr Asp Lys Thr Gln Pro Asn Pro Asn Ile Ala Asn Val Thr Ala

65 70 75 80

Gly Leu Lys Val Leu Lys Glu Gln Asn Ser Glu Ile Val Val Ser Ile

85 90 95

Gly Gly Gly Ser Ala His Asp Asn Ala Lys Ala Ile Ala Leu Leu Ala

100 105 110

Thr Asn Gly Gly Glu Ile Gly Asp Tyr Glu Gly Val Asn Gln Ser Lys

115 120 125

Lys Ala Ala Leu Pro Leu Phe Ala Ile Asn Thr Thr Ala Gly Thr Ala

130 135 140

Ser Glu Met Thr Arg Phe Thr Ile Ile Ser Asn Glu Glu Lys Lys Ile

145 150 155 160

Lys Met Ala Ile Ile Asp Asn Asn Val Thr Pro Ala Val Ala Val Asn

165 170 175

Asp Pro Ser Thr Met Phe Gly Leu Pro Pro Ala Leu Thr Ala Ala Thr

180 185 190

Gly Leu Asp Ala Leu Thr His Cys Ile Glu Ala Tyr Val Ser Thr Ala

195 200 205

Ser Asn Pro Ile Thr Asp Ala Cys Ala Leu Lys Gly Ile Asp Leu Ile

210 215 220

Asn Glu Ser Leu Val Ala Ala Tyr Lys Asp Gly Lys Asp Lys Lys Ala

225 230 235 240

Arg Thr Asp Met Cys Tyr Ala Glu Tyr Leu Ala Gly Met Ala Phe Asn

245 250 255

Asn Ala Ser Leu Gly Tyr Val His Ala Leu Ala His Gln Leu Gly Gly

260 265 270

Phe Tyr His Leu Pro His Gly Val Cys Asn Ala Val Leu Leu Pro His

275 280 285

Val Gln Glu Ala Asn Met Gln Cys Pro Lys Ala Lys Lys Arg Leu Gly

290 295 300

Glu Ile Ala Leu His Phe Gly Ala Ser Gln Glu Asp Pro Glu Glu Thr

305 310 315 320

Ile Lys Ala Leu His Val Leu Asn Arg Thr Met Asn Ile Pro Arg Asn

325 330 335

Leu Lys Glu Leu Gly Val Lys Thr Glu Asp Phe Glu Ile Leu Ala Glu

340 345 350

His Ala Met His Asp Ala Cys His Leu Thr Asn Pro Val Gln Phe Thr

355 360 365

Lys Glu Gln Val Val Ala Ile Ile Lys Lys Ala Tyr Glu Tyr

370 375 380

<210> 114

<211> 331

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 114

Met Glu Tyr Thr Ser Ile Ala Asp Thr Gly Ile Glu Ala Ser Arg Ile

1 5 10 15

Gly Leu Gly Thr Trp Ala Ile Gly Gly Thr Met Trp Gly Gly Thr Asp

20 25 30

Glu Lys Thr Ser Ile Glu Thr Ile Arg Ala Ala Leu Asp Gln Gly Ile

35 40 45

Thr Leu Ile Asp Thr Ala Pro Ala Tyr Gly Phe Gly Gln Ser Glu Glu

50 55 60

Ile Val Gly Lys Ala Ile Lys Glu Tyr Gly Lys Arg Asp Gln Val Ile

65 70 75 80

Leu Ala Thr Lys Thr Ala Leu Asp Trp Lys Asn Asn Gln Leu Phe Arg

85 90 95

His Ala Asn Arg Ala Arg Ile Val Glu Glu Val Glu Asn Ser Leu Lys

100 105 110

Arg Leu Gln Thr Asp Tyr Ile Asp Leu Tyr Gln Val His Trp Pro Asp

115 120 125

Pro Leu Val Pro Ile Glu Glu Thr Ala Glu Val Met Lys Glu Leu Tyr

130 135 140

Asp Ala Gly Lys Ile Arg Ala Ile Gly Val Ser Asn Phe Ser Ile Glu

145 150 155 160

Gln Met Asp Thr Phe Arg Ala Val Ala Pro Leu His Thr Ile Gln Pro

165 170 175

Pro Tyr Asn Leu Phe Glu Arg Glu Met Glu Glu Ser Val Leu Pro Tyr

180 185 190

Ala Lys Asp Asn Lys Ile Thr Thr Leu Leu Tyr Gly Ser Leu Cys Arg

195 200 205

Gly Leu Leu Thr Gly Lys Met Thr Glu Glu Tyr Thr Phe Glu Gly Asp

210 215 220

Asp Leu Arg Asn His Asp Pro Lys Phe Gln Lys Pro Arg Phe Lys Glu

225 230 235 240

Tyr Leu Ser Ala Val Asn Gln Leu Asp Lys Leu Ala Lys Thr Arg Tyr

245 250 255

Gly Lys Ser Val Ile His Leu Ala Val Arg Trp Ile Leu Asp Gln Pro

260 265 270

Gly Ala Asp Ile Ala Leu Trp Gly Ala Arg Lys Pro Gly Gln Leu Glu

275 280 285

Ala Leu Ser Glu Ile Thr Gly Trp Thr Leu Asn Ser Glu Asp Gln Lys

290 295 300

Asp Ile Asn Thr Ile Leu Glu Asn Thr Ile Ser Asp Pro Val Gly Pro

305 310 315 320

Glu Phe Met Ala Pro Pro Thr Arg Glu Glu Ile

325 330

<210> 115

<211> 332

<212> PRT

<213> 氧化葡糖酸杆菌(Gluconobacter oxydans)

<400> 115

Met Ala Ser Asp Thr Ile Arg Ile Pro Gly Ile Asp Thr Pro Leu Ser

1 5 10 15

Arg Val Ala Leu Gly Thr Trp Ala Ile Gly Gly Trp Met Trp Gly Gly

20 25 30

Pro Asp Asp Asp Asn Gly Val Arg Thr Ile His Ala Ala Leu Asp Glu

35 40 45

Gly Ile Asn Leu Ile Asp Thr Ala Pro Val Tyr Gly Phe Gly His Ser

50 55 60

Glu Glu Ile Val Gly Arg Ala Leu Ala Glu Lys Pro Asn Lys Ala His

65 70 75 80

Val Ala Thr Lys Leu Gly Leu His Trp Val Gly Glu Asp Glu Lys Asn

85 90 95

Met Lys Val Phe Arg Asp Ser Arg Pro Ala Arg Ile Arg Lys Glu Val

100 105 110

Glu Asp Ser Leu Arg Arg Leu Arg Val Glu Thr Ile Asp Leu Glu Gln

115 120 125

Ile His Trp Pro Asp Asp Lys Thr Pro Ile Asp Glu Ser Ala Arg Glu

130 135 140

Leu Gln Lys Leu His Gln Asp Gly Lys Ile Arg Ala Leu Gly Val Ser

145 150 155 160

Asn Phe Ser Pro Glu Gln Met Asp Ile Phe Arg Glu Val Ala Pro Leu

165 170 175

Ala Thr Ile Gln Pro Pro Leu Asn Leu Phe Glu Arg Thr Ile Glu Lys

180 185 190

Asp Ile Leu Pro Tyr Ala Glu Lys His Asn Ala Val Val Leu Ala Tyr

195 200 205

Gly Ala Leu Cys Arg Gly Leu Leu Thr Gly Lys Met Asn Arg Asp Thr

210 215 220

Thr Phe Pro Lys Asp Asp Leu Arg Ser Asn Asp Pro Lys Phe Gln Lys

225 230 235 240

Pro Asn Phe Glu Lys Tyr Leu Ala Ala Met Asp Glu Phe Glu Lys Leu

245 250 255

Ala Glu Lys Arg Gly Lys Ser Val Met Ala Phe Ala Val Arg Trp Val

260 265 270

Leu Asp Gln Gly Pro Val Ile Ala Leu Trp Gly Ala Arg Lys Pro Gly

275 280 285

Gln Val Ser Gly Val Lys Asp Val Phe Gly Trp Ser Leu Thr Asp Glu

290 295 300

Glu Lys Lys Ala Val Asp Asp Ile Leu Ala Arg His Val Pro Asn Pro

305 310 315 320

Ile Asp Pro Thr Phe Met Ala Pro Pro Ala Arg Asp

325 330

<210> 116

<211> 339

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 116

Met Ser Met Ile Lys Ser Tyr Ala Ala Lys Glu Ala Gly Gly Glu Leu

1 5 10 15

Glu Val Tyr Glu Tyr Asp Pro Gly Glu Leu Arg Pro Gln Asp Val Glu

20 25 30

Val Gln Val Asp Tyr Cys Gly Ile Cys His Ser Asp Leu Ser Met Ile

35 40 45

Asp Asn Glu Trp Gly Phe Ser Gln Tyr Pro Leu Val Ala Gly His Glu

50 55 60

Val Ile Gly Arg Val Val Ala Leu Gly Ser Ala Ala Gln Asp Lys Gly

65 70 75 80

Leu Gln Val Gly Gln Arg Val Gly Ile Gly Trp Thr Ala Arg Ser Cys

85 90 95

Gly His Cys Asp Ala Cys Ile Ser Gly Asn Gln Ile Asn Cys Glu Gln

100 105 110

Gly Ala Val Pro Thr Ile Met Asn Arg Gly Gly Phe Ala Glu Lys Leu

115 120 125

Arg Ala Asp Trp Gln Trp Val Ile Pro Leu Pro Glu Asn Ile Asp Ile

130 135 140

Glu Ser Ala Gly Pro Leu Leu Cys Gly Gly Ile Thr Val Phe Lys Pro

145 150 155 160

Leu Leu Met His His Ile Thr Ala Thr Ser Arg Val Gly Val Ile Gly

165 170 175

Ile Gly Gly Leu Gly His Ile Ala Ile Lys Leu Leu His Ala Met Gly

180 185 190

Cys Glu Val Thr Ala Phe Ser Ser Asn Pro Ala Lys Glu Gln Glu Val

195 200 205

Leu Ala Met Gly Ala Asp Lys Val Val Asn Ser Arg Asp Pro Gln Ala

210 215 220

Leu Lys Ala Leu Ala Gly Gln Phe Asp Leu Ile Ile Asn Thr Val Asn

225 230 235 240

Val Ser Leu Asp Trp Gln Pro Tyr Phe Glu Ala Leu Thr Tyr Gly Gly

245 250 255

Asn Phe His Thr Val Gly Ala Val Leu Thr Pro Leu Ser Val Pro Ala

260 265 270

Phe Thr Leu Ile Ala Gly Asp Arg Ser Val Ser Gly Ser Ala Thr Gly

275 280 285

Thr Pro Tyr Glu Leu Arg Lys Leu Met Arg Phe Ala Ala Arg Ser Lys

290 295 300

Val Ala Pro Thr Thr Glu Leu Phe Pro Met Ser Lys Ile Asn Asp Ala

305 310 315 320

Ile Gln His Val Arg Asp Gly Lys Ala Arg Tyr Arg Val Val Leu Lys

325 330 335

Ala Asp Phe

<210> 117

<211> 315

<212> PRT

<213> 拟南芥(Arabidopsis thaliana)

<400> 117

Met Ala Asn Ala Ile Thr Phe Phe Lys Leu Asn Thr Gly Ala Lys Phe

1 5 10 15

Pro Ser Val Gly Leu Gly Thr Trp Gln Ala Ser Pro Gly Leu Val Gly

20 25 30

Asp Ala Val Ala Ala Ala Val Lys Ile Gly Tyr Arg His Ile Asp Cys

35 40 45

Ala Gln Ile Tyr Gly Asn Glu Lys Glu Ile Gly Ala Val Leu Lys Lys

50 55 60

Leu Phe Glu Asp Arg Val Val Lys Arg Glu Asp Leu Phe Ile Thr Ser

65 70 75 80

Lys Leu Trp Cys Thr Asp His Asp Pro Gln Asp Val Pro Glu Ala Leu

85 90 95

Asn Arg Thr Leu Lys Asp Leu Gln Leu Glu Tyr Val Asp Leu Tyr Leu

100 105 110

Ile His Trp Pro Ala Arg Ile Lys Lys Gly Ser Val Gly Ile Lys Pro

115 120 125

Glu Asn Leu Leu Pro Val Asp Ile Pro Ser Thr Trp Lys Ala Met Glu

130 135 140

Ala Leu Tyr Asp Ser Gly Lys Ala Arg Ala Ile Gly Val Ser Asn Phe

145 150 155 160

Ser Thr Lys Lys Leu Ala Asp Leu Leu Glu Leu Ala Arg Val Pro Pro

165 170 175

Ala Val Asn Gln Val Glu Cys His Pro Ser Trp Arg Gln Thr Lys Leu

180 185 190

Gln Glu Phe Cys Lys Ser Lys Gly Val His Leu Ser Ala Tyr Ser Pro

195 200 205

Leu Gly Ser Pro Gly Thr Thr Trp Leu Lys Ser Asp Val Leu Lys Asn

210 215 220

Pro Ile Leu Asn Met Val Ala Glu Lys Leu Gly Lys Ser Pro Ala Gln

225 230 235 240

Val Ala Leu Arg Trp Gly Leu Gln Met Gly His Ser Val Leu Pro Lys

245 250 255

Ser Thr Asn Glu Gly Arg Ile Lys Glu Asn Phe Asn Val Phe Asp Trp

260 265 270

Ser Ile Pro Asp Tyr Met Phe Ala Lys Phe Ala Glu Ile Glu Gln Ala

275 280 285

Arg Leu Val Thr Gly Ser Phe Leu Val His Glu Thr Leu Ser Pro Tyr

290 295 300

Lys Ser Ile Glu Glu Leu Trp Asp Gly Glu Ile

305 310 315

<210> 118

<211> 284

<212> PRT

<213> 杜氏利什曼虫(Leishmania donovani)

<400> 118

Met Ala Asp Val Gly Lys Ala Met Val Thr Leu Ser Asn Gly Val Gln

1 5 10 15

Met Pro Gln Leu Gly Leu Gly Val Trp Gln Ser Pro Ala Gly Glu Val

20 25 30

Thr Ala Asn Ala Val Lys Trp Ala Leu Cys Ala Gly Tyr Arg His Ile

35 40 45

Asp Thr Ala Ala Ile Tyr Lys Asn Glu Glu Ser Val Gly Ala Gly Leu

50 55 60

Arg Ala Ser Gly Val Pro Arg Glu Asp Val Phe Ile Thr Thr Lys Leu

65 70 75 80

Trp Asn Thr Glu Gln Gly Tyr Glu Ser Thr Leu Ala Ala Phe Glu Glu

85 90 95

Ser Arg Gln Lys Leu Gly Val Asp Tyr Ile Asp Leu Tyr Leu Ile His

100 105 110

Trp Pro Arg Gly Lys Asp Ile Val Ser Lys Glu Gly Lys Lys Tyr Leu

115 120 125

Asp Ser Trp Arg Ala Phe Glu Gln Leu Tyr Lys Asp Lys Lys Val Arg

130 135 140

Ala Ile Gly Val Ser Asn Phe His Ile His His Leu Glu Asp Val Leu

145 150 155 160

Ala Met Cys Thr Val Thr Pro Met Val Asn Gln Val Glu Leu His Pro

165 170 175

Leu Asn Asn Gln Ala Glu Leu Arg Ala Phe Cys Asp Ala Lys Gln Ile

180 185 190

Lys Val Glu Ala Trp Ser Pro Leu Gly Gln Gly Lys Leu Leu Ser Asn

195 200 205

Pro Ile Leu Ala Ala Ile Gly Ala Lys Tyr Asn Lys Thr Ala Ala Gln

210 215 220

Val Ile Leu Arg Trp Asn Ile Gln Lys Asn Leu Ile Thr Ile Pro Lys

225 230 235 240

Ser Val His Lys Glu Arg Ile Glu Glu Asn Ala Asp Val Phe Asn Phe

245 250 255

Glu Leu Asp Ala Glu Asp Val Met Ser Ile Asp Ala Leu Asn Thr Asn

260 265 270

Ser Arg Tyr Gly Pro Asp Pro Asp Glu Ala Gln Phe

275 280

<210> 119

<211> 349

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 119

Met Lys Ile Lys Ala Val Gly Ala Tyr Ser Ala Lys Gln Pro Leu Glu

1 5 10 15

Pro Met Asp Ile Thr Arg Arg Glu Pro Gly Pro Asn Asp Val Lys Ile

20 25 30

Glu Ile Ala Tyr Cys Gly Val Cys His Ser Asp Leu His Gln Val Arg

35 40 45

Ser Glu Trp Ala Gly Thr Val Tyr Pro Cys Val Pro Gly His Glu Ile

50 55 60

Val Gly Arg Val Val Ala Val Gly Asp Gln Val Glu Lys Tyr Ala Pro

65 70 75 80

Gly Asp Leu Val Gly Val Gly Cys Ile Val Asp Ser Cys Lys His Cys

85 90 95

Glu Glu Cys Glu Asp Gly Leu Glu Asn Tyr Cys Asp His Met Thr Gly

100 105 110

Thr Tyr Asn Ser Pro Thr Pro Asp Glu Pro Gly His Thr Leu Gly Gly

115 120 125

Tyr Ser Gln Gln Ile Val Val His Glu Arg Tyr Val Leu Arg Ile Arg

130 135 140

His Pro Gln Glu Gln Leu Ala Ala Val Ala Pro Leu Leu Cys Ala Gly

145 150 155 160

Ile Thr Thr Tyr Ser Pro Leu Arg His Trp Gln Ala Gly Pro Gly Lys

165 170 175

Lys Val Gly Val Val Gly Ile Gly Gly Leu Gly His Met Gly Ile Lys

180 185 190

Leu Ala His Ala Met Gly Ala His Val Val Ala Phe Thr Thr Ser Glu

195 200 205

Ala Lys Arg Glu Ala Ala Lys Ala Leu Gly Ala Asp Glu Val Val Asn

210 215 220

Ser Arg Asn Ala Asp Glu Met Ala Ala His Leu Lys Ser Phe Asp Phe

225 230 235 240

Ile Leu Asn Thr Val Ala Ala Pro His Asn Leu Asp Asp Phe Thr Thr

245 250 255

Leu Leu Lys Arg Asp Gly Thr Met Thr Leu Val Gly Ala Pro Ala Thr

260 265 270

Pro His Lys Ser Pro Glu Val Phe Asn Leu Ile Met Lys Arg Arg Ala

275 280 285

Ile Ala Gly Ser Met Ile Gly Gly Ile Pro Glu Thr Gln Glu Met Leu

290 295 300

Asp Phe Cys Ala Glu His Gly Ile Val Ala Asp Ile Glu Met Ile Arg

305 310 315 320

Ala Asp Gln Ile Asn Glu Ala Tyr Glu Arg Met Leu Arg Gly Asp Val

325 330 335

Lys Tyr Arg Phe Val Ile Asp Asn Arg Thr Leu Thr Asp

340 345

<210> 120

<211> 346

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 120

Met Gln Tyr His Arg Ile Pro His Ser Ser Leu Glu Val Ser Thr Leu

1 5 10 15

Gly Leu Gly Thr Met Thr Phe Gly Glu Gln Asn Ser Glu Ala Asp Ala

20 25 30

His Ala Gln Leu Asp Tyr Ala Val Ala Gln Gly Ile Asn Leu Ile Asp

35 40 45

Val Ala Glu Met Tyr Pro Val Pro Pro Arg Pro Glu Thr Gln Gly Leu

50 55 60

Thr Glu Thr Tyr Val Gly Asn Trp Leu Ala Lys His Gly Ser Arg Glu

65 70 75 80

Lys Leu Ile Ile Ala Ser Lys Val Ser Gly Pro Ser Arg Asn Asn Asp

85 90 95

Lys Gly Ile Arg Pro Asp Gln Ala Leu Asp Arg Lys Asn Ile Arg Glu

100 105 110

Ala Leu His Asp Ser Leu Lys Arg Leu Gln Thr Asp Tyr Leu Asp Leu

115 120 125

Tyr Gln Val His Trp Pro Gln Arg Pro Thr Asn Cys Phe Gly Lys Leu

130 135 140

Gly Tyr Ser Trp Thr Asp Ser Ala Pro Ala Val Ser Leu Leu Asp Thr

145 150 155 160

Leu Asp Ala Leu Ala Glu Tyr Gln Arg Ala Gly Lys Ile Arg Tyr Ile

165 170 175

Gly Val Ser Asn Glu Thr Ala Phe Gly Val Met Arg Tyr Leu His Leu

180 185 190

Ala Asp Lys His Asp Leu Pro Arg Ile Val Thr Ile Gln Asn Pro Tyr

195 200 205

Ser Leu Leu Asn Arg Ser Phe Glu Val Gly Leu Ala Glu Val Ser Gln

210 215 220

Tyr Glu Gly Val Glu Leu Leu Ala Tyr Ser Cys Leu Gly Phe Gly Thr

225 230 235 240

Leu Thr Gly Lys Tyr Leu Asn Gly Ala Lys Pro Ala Gly Ala Arg Asn

245 250 255

Thr Leu Phe Ser Arg Phe Thr Arg Tyr Ser Gly Glu Gln Thr Gln Lys

260 265 270

Ala Val Ala Ala Tyr Val Asp Ile Ala Arg Arg His Gly Leu Asp Pro

275 280 285

Ala Gln Met Ala Leu Ala Phe Val Arg Arg Gln Pro Phe Val Ala Ser

290 295 300

Thr Leu Leu Gly Ala Thr Thr Met Asp Gln Leu Lys Thr Asn Ile Glu

305 310 315 320

Ser Leu His Leu Glu Leu Ser Glu Asp Val Leu Ala Glu Ile Glu Ala

325 330 335

Val His Gln Val Tyr Thr Tyr Pro Ala Pro

340 345

<210> 121

<211> 399

<212> PRT

<213> 产乙醇热厌氧杆菌(Thermoanaerobacter ethaolicus)

<400> 121

Met Trp Glu Thr Lys Ile Asn Pro Asn Lys Val Phe Glu Leu Arg Cys

1 5 10 15

Lys Asn Thr Thr Tyr Phe Gly Ile Gly Ser Ile Lys Lys Ile Lys Asp

20 25 30

Ile Leu Glu Val Leu Lys Asn Lys Gly Ile Asn Asn Val Ile Leu Val

35 40 45

Thr Gly Lys Gly Ser Tyr Lys Ala Ser Gly Ala Trp Asp Val Val Lys

50 55 60

Pro Ala Leu Glu Thr Leu Gly Phe Lys Tyr Ser Leu Tyr Asp Lys Val

65 70 75 80

Gly Pro Asn Pro Thr Val Asp Met Ile Asp Glu Ala Ala Lys Ile Gly

85 90 95

Arg Glu Thr Gly Ala Lys Ala Val Ile Gly Ile Gly Gly Gly Ser Pro

100 105 110

Ile Asp Thr Ala Lys Ser Val Ala Val Leu Leu Glu Tyr Thr Asp Lys

115 120 125

Asn Ala Arg Glu Leu Tyr Glu Gln Lys Phe Ile Pro Glu Lys Ala Ala

130 135 140

Pro Ile Ile Ala Ile Asn Leu Thr His Gly Thr Gly Thr Glu Val Asp

145 150 155 160

Arg Phe Ala Val Ala Thr Ile Pro Glu Lys Asn Tyr Lys Pro Ala Ile

165 170 175

Ala Tyr Asp Cys Leu Tyr Pro Met Tyr Ala Ile Asp Asp Pro Ser Leu

180 185 190

Met Thr Lys Leu Asp Lys Lys Gln Thr Ile Ala Val Thr Ile Asp Ala

195 200 205

Leu Asn His Val Thr Glu Ala Ala Thr Thr Leu Val Ala Ser Pro Tyr

210 215 220

Ser Val Leu Met Ala Lys Glu Thr Val Arg Leu Ile Val Arg Tyr Leu

225 230 235 240

Pro Ala Ala Val Asn Asp Pro Glu Asn Leu Val Ala Arg Tyr Tyr Leu

245 250 255

Leu Tyr Ala Ser Ala Leu Ala Gly Ile Ser Phe Asp Asn Gly Leu Leu

260 265 270

His Leu Thr His Ala Leu Glu His Pro Leu Ser Ala Val Lys Pro Glu

275 280 285

Ile Ala His Gly Leu Gly Leu Gly Ala Ile Leu Pro Ala Val Val Lys

290 295 300

Ala Ile Tyr Pro Ser Val Ala Glu Val Leu Ala Glu Val Tyr Ser Pro

305 310 315 320

Ile Val Pro Gly Leu Lys Gly Leu Pro Ala Glu Ala Glu Tyr Val Ala

325 330 335

Lys Lys Val Glu Glu Trp Leu Phe Lys Val Gly Cys Thr Gln Lys Leu

340 345 350

Ser Asp Phe Gly Phe Thr Lys Glu Asp Ile Pro Thr Leu Val Arg Leu

355 360 365

Ala Lys Thr Thr Pro Ser Leu Asp Gly Leu Leu Ser Asn Ala Pro Val

370 375 380

Glu Ala Thr Glu Ala Val Ile Ala Lys Ile Tyr Glu Glu Ser Phe

385 390 395

<210> 122

<211> 346

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 122

Met Lys Ala Ala Arg Trp His Asn Gln Lys Asp Ile Arg Ile Glu His

1 5 10 15

Ile Glu Glu Pro Lys Thr Glu Pro Gly Lys Val Lys Ile Lys Val Lys

20 25 30

Trp Cys Gly Ile Cys Gly Ser Asp Leu His Glu Tyr Leu Gly Gly Pro

35 40 45

Ile Phe Ile Pro Val Asp Lys Pro His Pro Leu Thr Asn Glu Thr Ala

50 55 60

Pro Val Thr Met Gly His Glu Phe Ser Gly Glu Val Val Glu Val Gly

65 70 75 80

Glu Gly Val Glu Asn Tyr Lys Val Gly Asp Arg Val Val Val Glu Pro

85 90 95

Ile Phe Ala Thr His Gly His Gln Gly Ala Tyr Asn Leu Asp Glu Gln

100 105 110

Met Gly Phe Leu Gly Leu Ala Gly Gly Gly Gly Gly Phe Ser Glu Tyr

115 120 125

Val Ser Val Asp Glu Glu Leu Leu Phe Lys Leu Pro Asp Glu Leu Ser

130 135 140

Tyr Glu Gln Gly Ala Leu Val Glu Pro Ser Ala Val Ala Leu Tyr Ala

145 150 155 160

Val Arg Ser Ser Lys Leu Lys Ala Gly Asp Lys Ala Ala Val Phe Gly

165 170 175

Cys Gly Pro Ile Gly Leu Leu Val Ile Glu Ala Leu Lys Ala Ala Gly

180 185 190

Ala Thr Asp Ile Tyr Ala Val Glu Leu Ser Pro Glu Arg Gln Gln Lys

195 200 205

Ala Glu Glu Leu Gly Ala Ile Ile Val Asp Pro Ser Lys Thr Asp Asp

210 215 220

Val Val Ala Glu Ile Ala Glu Arg Thr Gly Gly Gly Val Asp Val Ala

225 230 235 240

Phe Glu Val Thr Gly Val Pro Val Val Leu Arg Gln Ala Ile Gln Ser

245 250 255

Thr Thr Ile Ala Gly Glu Thr Val Ile Val Ser Ile Trp Glu Lys Gly

260 265 270

Ala Glu Ile His Pro Asn Asp Ile Val Ile Lys Glu Arg Thr Val Lys

275 280 285

Gly Ile Ile Gly Tyr Arg Asp Ile Phe Pro Ala Val Leu Ser Leu Met

290 295 300

Lys Glu Gly Tyr Phe Ser Ala Asp Lys Leu Val Thr Lys Lys Ile Val

305 310 315 320

Leu Asp Asp Leu Ile Glu Glu Gly Phe Gly Ala Leu Ile Lys Glu Lys

325 330 335

Ser Gln Val Lys Ile Leu Val Arg Pro Asn

340 345

<210> 123

<211> 382

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 123

Met Arg Ala Leu Ala Tyr Phe Lys Lys Gly Asp Ile His Phe Thr Asn

1 5 10 15

Asp Ile Pro Arg Pro Glu Ile Gln Thr Asp Asp Glu Val Ile Ile Asp

20 25 30

Val Ser Trp Cys Gly Ile Cys Gly Ser Asp Leu His Glu Tyr Leu Asp

35 40 45

Gly Pro Ile Phe Met Pro Lys Asp Gly Glu Cys His Lys Leu Ser Asn

50 55 60

Ala Ala Leu Pro Leu Ala Met Gly His Glu Met Ser Gly Ile Val Ser

65 70 75 80

Lys Val Gly Pro Lys Val Thr Lys Val Lys Val Gly Asp His Val Val

85 90 95

Val Asp Ala Ala Ser Ser Cys Ala Asp Leu His Cys Trp Pro His Ser

100 105 110

Lys Phe Tyr Asn Ser Lys Pro Cys Asp Ala Cys Gln Arg Gly Ser Glu

115 120 125

Asn Leu Cys Thr His Ala Gly Phe Val Gly Leu Gly Val Ile Ser Gly

130 135 140

Gly Phe Ala Glu Gln Val Val Val Ser Gln His His Ile Ile Pro Val

145 150 155 160

Pro Lys Glu Ile Pro Leu Asp Val Ala Ala Leu Val Glu Pro Leu Ser

165 170 175

Val Thr Trp His Ala Val Lys Ile Ser Gly Phe Lys Lys Gly Ser Ser

180 185 190

Ala Leu Val Leu Gly Ala Gly Pro Ile Gly Leu Cys Thr Ile Leu Val

195 200 205

Leu Lys Gly Met Gly Ala Ser Lys Ile Val Val Ser Glu Ile Ala Glu

210 215 220

Arg Arg Ile Glu Met Ala Lys Lys Leu Gly Val Glu Val Phe Asn Pro

225 230 235 240

Ser Lys His Gly His Lys Ser Ile Glu Ile Leu Arg Gly Leu Thr Lys

245 250 255

Ser His Asp Gly Phe Asp Tyr Ser Tyr Asp Cys Ser Gly Ile Gln Val

260 265 270

Thr Phe Glu Thr Ser Leu Lys Ala Leu Thr Phe Lys Gly Thr Ala Thr

275 280 285

Asn Ile Ala Val Trp Gly Pro Lys Pro Val Pro Phe Gln Pro Met Asp

290 295 300

Val Thr Leu Gln Glu Lys Val Met Thr Gly Ser Ile Gly Tyr Val Val

305 310 315 320

Glu Asp Phe Glu Glu Val Val Arg Ala Ile His Asn Gly Asp Ile Ala

325 330 335

Met Glu Asp Cys Lys Gln Leu Ile Thr Gly Lys Gln Arg Ile Glu Asp

340 345 350

Gly Trp Glu Lys Gly Phe Gln Glu Leu Met Asp His Lys Glu Ser Asn

355 360 365

Val Lys Ile Leu Leu Thr Pro Asn Asn His Gly Glu Met Lys

370 375 380

<210> 124

<211> 258

<212> PRT

<213> 谷氨酸棒状杆菌(Corynebacterium glutamicum)

<400> 124

Met Ser Lys Val Ala Met Val Thr Gly Gly Ala Gln Gly Ile Gly Arg

1 5 10 15

Gly Ile Ser Glu Lys Leu Ala Ala Asp Gly Phe Asp Ile Ala Val Ala

20 25 30

Asp Leu Pro Gln Gln Glu Glu Gln Ala Ala Glu Thr Ile Lys Leu Ile

35 40 45

Glu Ala Ala Gly Gln Lys Ala Val Phe Val Gly Leu Asp Val Thr Asp

50 55 60

Lys Ala Asn Phe Asp Ser Ala Ile Asp Glu Ala Ala Glu Lys Leu Gly

65 70 75 80

Gly Phe Asp Val Leu Val Asn Asn Ala Gly Ile Ala Gln Ile Lys Pro

85 90 95

Leu Leu Glu Val Thr Glu Glu Asp Leu Lys Gln Ile Tyr Ser Val Asn

100 105 110

Val Phe Ser Val Phe Phe Gly Ile Gln Ala Ala Ser Arg Lys Phe Asp

115 120 125

Glu Leu Gly Val Lys Gly Lys Ile Ile Asn Ala Ala Ser Ile Ala Ala

130 135 140

Ile Gln Gly Phe Pro Ile Leu Ser Ala Tyr Ser Thr Thr Lys Phe Ala

145 150 155 160

Val Arg Gly Leu Thr Gln Ala Ala Ala Gln Glu Leu Ala Pro Lys Gly

165 170 175

His Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Gly Thr Gly Met Trp

180 185 190

Glu Gln Ile Asp Ala Glu Leu Ser Lys Ile Asn Gly Lys Pro Ile Gly

195 200 205

Glu Asn Phe Lys Glu Tyr Ser Ser Ser Ile Ala Leu Gly Arg Pro Ser

210 215 220

Val Pro Glu Asp Val Ala Gly Leu Val Ser Phe Leu Ala Ser Glu Asn

225 230 235 240

Ser Asn Tyr Ile Thr Gly Gln Val Met Leu Val Asp Gly Gly Met Leu

245 250 255

Tyr Asn

<210> 125

<211> 256

<212> PRT

<213> 肺炎克雷伯氏菌(Klebsiella pneumoniae)

<400> 125

Met Lys Lys Val Ala Leu Val Thr Gly Ala Gly Gln Gly Ile Gly Lys

1 5 10 15

Ala Ile Ala Leu Arg Leu Val Lys Asp Gly Phe Ala Val Ala Ile Ala

20 25 30

Asp Tyr Asn Asp Ala Thr Ala Lys Ala Val Ala Ser Glu Ile Asn Gln

35 40 45

Ala Gly Gly Arg Ala Met Ala Val Lys Val Asp Val Ser Asp Arg Asp

50 55 60

Gln Val Phe Ala Ala Val Glu Gln Ala Arg Lys Thr Leu Gly Gly Phe

65 70 75 80

Asp Val Ile Val Asn Asn Ala Gly Val Ala Pro Ser Thr Pro Ile Glu

85 90 95

Ser Ile Thr Pro Glu Ile Val Asp Lys Val Tyr Asn Ile Asn Val Lys

100 105 110

Gly Val Ile Trp Gly Ile Gln Ala Ala Val Glu Ala Phe Lys Lys Glu

115 120 125

Gly His Gly Gly Lys Ile Ile Asn Ala Cys Ser Gln Ala Gly His Val

130 135 140

Gly Asn Pro Glu Leu Ala Val Tyr Ser Ser Ser Lys Phe Ala Val Arg

145 150 155 160

Gly Leu Thr Gln Thr Ala Ala Arg Asp Leu Ala Pro Leu Gly Ile Thr

165 170 175

Val Asn Gly Tyr Cys Pro Gly Ile Val Lys Thr Pro Met Trp Ala Glu

180 185 190

Ile Asp Arg Gln Val Ser Glu Ala Ala Gly Lys Pro Leu Gly Tyr Gly

195 200 205

Thr Ala Glu Phe Ala Lys Arg Ile Thr Leu Gly Arg Leu Ser Glu Pro

210 215 220

Glu Asp Val Ala Ala Cys Val Ser Tyr Leu Ala Ser Pro Asp Ser Asp

225 230 235 240

Tyr Met Thr Gly Gln Ser Leu Leu Ile Asp Gly Gly Met Val Phe Asn

245 250 255

<210> 126

<211> 251

<212> PRT

<213> 粘质沙雷氏菌(Serratia marcescens)

<400> 126

Met Arg Phe Asp Asn Lys Val Val Val Ile Thr Gly Ala Gly Thr Gly

1 5 10 15

Met Gly Glu Ala Ala Ala Arg Arg Phe Ser Ala Glu Gly Ala Ile Val

20 25 30

Val Leu Ala Asp Trp Ala Lys Glu Ala Val Asp Lys Val Ala Ala Ser

35 40 45

Leu Pro Lys Gly Arg Ala Met Ala Val His Ile Asp Val Ser Asp His

50 55 60

Val Ala Val Glu Lys Met Met Asn Glu Val Ala Glu Lys Leu Gly Arg

65 70 75 80

Ile Asp Val Leu Leu Asn Asn Ala Gly Val His Val Ala Gly Ser Val

85 90 95

Leu Glu Thr Ser Val Asp Asp Trp Arg Arg Ile Ala Gly Val Asp Ile

100 105 110

Asp Gly Val Val Phe Cys Ser Lys Phe Ala Leu Pro His Leu Leu Lys

115 120 125

Thr Lys Gly Cys Ile Val Asn Thr Ala Ser Val Ser Gly Leu Gly Gly

130 135 140

Asp Trp Gly Ala Ala Tyr Tyr Cys Ala Ala Lys Gly Ala Val Val Asn

145 150 155 160

Leu Thr Arg Ala Met Ala Leu Asp His Gly Gly Asp Gly Val Arg Ile

165 170 175

Asn Ser Val Cys Pro Ser Leu Val Lys Thr Asn Met Thr Asn Gly Trp

180 185 190

Pro Gln Glu Ile Arg Asp Lys Phe Asn Glu Arg Ile Ala Leu Gly Arg

195 200 205

Ala Ala Glu Pro Glu Glu Val Ala Ala Val Met Ala Phe Leu Ala Ser

210 215 220

Asp Asp Ala Ser Phe Ile Asn Gly Ala Asn Ile Pro Val Asp Gly Gly

225 230 235 240

Ala Thr Ala Ser Asp Gly Gln Gln Asn Ile Val

245 250

<210> 127

<211> 367

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 127

Met Asp Arg Ile Ile Gln Ser Pro Gly Lys Tyr Ile Gln Gly Ala Asp

1 5 10 15

Val Ile Asn Arg Leu Gly Glu Tyr Leu Lys Pro Leu Ala Glu Arg Trp

20 25 30

Leu Val Val Gly Asp Lys Phe Val Leu Gly Phe Ala Gln Ser Thr Val

35 40 45

Glu Lys Ser Phe Lys Asp Ala Gly Leu Val Val Glu Ile Ala Pro Phe

50 55 60

Gly Gly Glu Cys Ser Gln Asn Glu Ile Asp Arg Leu Arg Gly Ile Ala

65 70 75 80

Glu Thr Ala Gln Cys Gly Ala Ile Leu Gly Ile Gly Gly Gly Lys Thr

85 90 95

Leu Asp Thr Ala Lys Ala Leu Ala His Phe Met Gly Val Pro Val Ala

100 105 110

Ile Ala Pro Thr Ile Ala Ser Thr Asp Ala Pro Cys Ser Ala Leu Ser

115 120 125

Val Ile Tyr Thr Asp Glu Gly Glu Phe Asp Arg Tyr Leu Leu Leu Pro

130 135 140

Asn Asn Pro Asn Met Val Ile Val Asp Thr Lys Ile Val Ala Gly Ala

145 150 155 160

Pro Ala Arg Leu Leu Ala Ala Gly Ile Gly Asp Ala Leu Ala Thr Trp

165 170 175

Phe Glu Ala Arg Ala Cys Ser Arg Ser Gly Ala Thr Thr Met Ala Gly

180 185 190

Gly Lys Cys Thr Gln Ala Ala Leu Ala Leu Ala Glu Leu Cys Tyr Asn

195 200 205

Thr Leu Leu Glu Glu Gly Glu Lys Ala Met Leu Ala Ala Glu Gln His

210 215 220

Val Val Thr Pro Ala Leu Glu Arg Val Ile Glu Ala Asn Thr Tyr Leu

225 230 235 240

Ser Gly Val Gly Phe Glu Ser Gly Gly Leu Ala Ala Ala His Ala Val

245 250 255

His Asn Gly Leu Thr Ala Ile Pro Asp Ala His His Tyr Tyr His Gly

260 265 270

Glu Lys Val Ala Phe Gly Thr Leu Thr Gln Leu Val Leu Glu Asn Ala

275 280 285

Pro Val Glu Glu Ile Glu Thr Val Ala Ala Leu Ser His Ala Val Gly

290 295 300

Leu Pro Ile Thr Leu Ala Gln Leu Asp Ile Lys Glu Asp Val Pro Ala

305 310 315 320

Lys Met Arg Ile Val Ala Glu Ala Ala Cys Ala Glu Gly Glu Thr Ile

325 330 335

His Asn Met Pro Gly Gly Ala Thr Pro Asp Gln Val Tyr Ala Ala Leu

340 345 350

Leu Val Ala Asp Gln Tyr Gly Gln Arg Phe Leu Gln Glu Trp Glu

355 360 365

<210> 128

<211> 258

<212> PRT

<213> 谷氨酸棒状杆菌(Corynebacterium glutamicum)

<400> 128

Met Ser Lys Val Ala Met Val Thr Gly Gly Ala Gln Gly Ile Gly Arg

1 5 10 15

Gly Ile Ser Glu Lys Leu Ala Ala Asp Gly Phe Asp Ile Ala Val Ala

20 25 30

Asp Leu Pro Gln Gln Glu Glu Gln Ala Ala Glu Thr Ile Lys Leu Ile

35 40 45

Glu Ala Ala Asp Gln Lys Ala Val Phe Val Gly Leu Asp Val Thr Asp

50 55 60

Lys Ala Asn Phe Asp Ser Ala Ile Asp Glu Ala Ala Glu Lys Leu Gly

65 70 75 80

Gly Phe Asp Val Leu Val Asn Asn Ala Gly Ile Ala Gln Ile Lys Pro

85 90 95

Leu Leu Glu Val Thr Glu Glu Asp Leu Lys Gln Ile Tyr Ser Val Asn

100 105 110

Val Phe Ser Val Phe Phe Gly Ile Gln Ala Ala Ser Arg Lys Phe Asp

115 120 125

Glu Leu Gly Val Lys Gly Lys Ile Ile Asn Ala Ala Ser Ile Ala Ala

130 135 140

Ile Gln Gly Phe Pro Ile Leu Ser Ala Tyr Ser Thr Thr Lys Phe Ala

145 150 155 160

Val Arg Gly Leu Thr Gln Ala Ala Ala Gln Glu Leu Ala Pro Lys Gly

165 170 175

His Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Gly Thr Gly Met Trp

180 185 190

Glu Gln Ile Asp Ala Glu Leu Ser Lys Ile Asn Gly Lys Pro Ile Gly

195 200 205

Glu Asn Phe Lys Glu Tyr Ser Ser Ser Ile Ala Leu Gly Arg Pro Ser

210 215 220

Val Pro Glu Asp Val Ala Gly Leu Val Ser Phe Leu Ala Ser Glu Asn

225 230 235 240

Ser Asn Tyr Val Thr Gly Gln Val Met Leu Val Asp Gly Gly Met Leu

245 250 255

Tyr Asn

<210> 129

<211> 318

<212> PRT

<213> 红褐肉座菌(Hypocrea jecorina)

<400> 129

Met Ala Ser Lys Thr Tyr Thr Leu Asn Thr Gly Ala Lys Ile Pro Ala

1 5 10 15

Val Gly Phe Gly Thr Phe Ala Asn Glu Gly Ala Lys Gly Glu Thr Tyr

20 25 30

Ala Ala Val Thr Lys Ala Leu Asp Val Gly Tyr Arg His Leu Asp Cys

35 40 45

Ala Trp Phe Tyr His Asn Glu Asp Glu Val Gly Asp Ala Val Arg Asp

50 55 60

Phe Leu Ala Arg Arg Pro Asp Val Lys Arg Glu Asp Leu Phe Ile Cys

65 70 75 80

Thr Lys Val Trp Asn His Leu His Glu Pro Glu Asp Val Lys Trp Ser

85 90 95

Ala Lys Asn Ser Cys Glu Asn Leu Lys Val Asp Tyr Ile Asp Leu Phe

100 105 110

Leu Val His Trp Pro Ile Ala Ala Glu Lys Asn Ser Asp Arg Ser Val

115 120 125

Lys Leu Gly Pro Asp Gly Lys Tyr Val Ile Asn Gln Ala Leu Thr Glu

130 135 140

Asn Pro Glu Pro Thr Trp Arg Ala Met Glu Glu Leu Val Glu Ser Gly

145 150 155 160

Leu Val Lys Ala Ile Gly Val Ser Asn Trp Thr Ile Pro Gly Leu Lys

165 170 175

Lys Leu Leu Gln Ile Ala Lys Ile Lys Pro Ala Val Asn Gln Ile Glu

180 185 190

Ile His Pro Phe Leu Pro Asn Glu Glu Leu Val Ala Phe Cys Phe Glu

195 200 205

Asn Gly Ile Leu Pro Glu Ala Tyr Ser Pro Leu Gly Ser Gln Asn Gln

210 215 220

Val Pro Ser Thr Gly Glu Arg Val Arg Asp Asn Pro Thr Leu Lys Ala

225 230 235 240

Val Ala Glu Arg Ser Gly Tyr Ser Leu Ala Gln Ile Leu Leu Ala Trp

245 250 255

Gly Leu Lys Arg Gly Tyr Val Val Leu Pro Lys Ser Ser Thr Pro Ser

260 265 270

Arg Ile Glu Ser Asn Phe Asn Ile Pro Glu Leu Ser Asp Glu Asp Phe

275 280 285

Glu Ala Ile Gln Gln Val Ala Lys Gly Arg His Thr Arg Phe Val Asn

290 295 300

Met Lys Asp Thr Phe Gly Tyr Asn Val Trp Pro Glu Glu Glu

305 310 315

<210> 130

<211> 385

<212> PRT

<213> 丁酸梭菌(Clostridium butyricum)

<400> 130

Met Arg Met Tyr Asp Tyr Leu Val Pro Ser Val Asn Phe Met Gly Ala

1 5 10 15

Asn Ser Val Ser Val Val Gly Glu Arg Cys Lys Ile Leu Gly Gly Lys

20 25 30

Lys Ala Leu Ile Val Thr Asp Lys Phe Leu Lys Asp Met Glu Gly Gly

35 40 45

Ala Val Glu Leu Thr Val Lys Tyr Leu Lys Glu Ala Gly Leu Asp Val

50 55 60

Val Tyr Tyr Asp Gly Val Glu Pro Asn Pro Lys Asp Val Asn Val Ile

65 70 75 80

Glu Gly Leu Lys Ile Phe Lys Glu Glu Asn Cys Asp Met Ile Val Thr

85 90 95

Val Gly Gly Gly Ser Ser His Asp Cys Gly Lys Gly Ile Gly Ile Ala

100 105 110

Ala Thr His Glu Gly Asp Leu Tyr Asp Tyr Ala Gly Ile Glu Thr Leu

115 120 125

Val Asn Pro Leu Pro Pro Ile Val Ala Val Asn Thr Thr Ala Gly Thr

130 135 140

Ala Ser Glu Leu Thr Arg His Cys Val Leu Thr Asn Thr Lys Lys Lys

145 150 155 160

Ile Lys Phe Val Ile Val Ser Trp Arg Asn Leu Pro Leu Val Ser Ile

165 170 175

Asn Asp Pro Met Leu Met Val Lys Lys Pro Ala Gly Leu Thr Ala Ala

180 185 190

Thr Gly Met Asp Ala Leu Thr His Ala Ile Glu Ala Tyr Val Ser Lys

195 200 205

Asp Ala Asn Pro Val Thr Asp Ala Ser Ala Ile Gln Ala Ile Lys Leu

210 215 220

Ile Ser Gln Asn Leu Arg Gln Ala Val Ala Leu Gly Glu Asn Leu Glu

225 230 235 240

Ala Arg Glu Asn Met Ala Tyr Ala Ser Leu Leu Ala Gly Met Ala Phe

245 250 255

Asn Asn Ala Asn Leu Gly Tyr Val His Ala Met Ala His Gln Leu Gly

260 265 270

Gly Leu Tyr Asp Met Pro His Gly Val Ala Asn Ala Met Leu Leu Pro

275 280 285

His Val Glu Arg Tyr Asn Met Leu Ser Asn Pro Lys Lys Phe Ala Asp

290 295 300

Ile Ala Glu Phe Met Gly Glu Asn Ile Ser Gly Leu Ser Val Met Glu

305 310 315 320

Ala Ala Glu Lys Ala Ile Asn Ala Met Phe Arg Leu Ser Glu Asp Val

325 330 335

Gly Ile Pro Lys Ser Leu Lys Glu Met Gly Val Lys Gln Glu Asp Phe

340 345 350

Glu His Met Ala Glu Leu Ala Leu Leu Asp Gly Asn Ala Phe Ser Asn

355 360 365

Pro Arg Lys Gly Asn Ala Lys Asp Ile Ile Asn Ile Phe Lys Ala Ala

370 375 380

Tyr

385

<210> 131

<211> 387

<212> PRT

<213> 弗氏柠檬酸杆菌(Citrobacter freundii)

<400> 131

Met Ser Tyr Arg Met Phe Asp Tyr Leu Val Pro Asn Val Asn Phe Phe

1 5 10 15

Gly Pro Asn Ala Ile Ser Val Val Gly Glu Arg Cys Lys Leu Leu Gly

20 25 30

Gly Lys Lys Ala Leu Leu Val Thr Asp Lys Gly Leu Arg Ala Ile Lys

35 40 45

Asp Gly Ala Val Asp Lys Thr Leu Thr His Leu Arg Glu Ala Gly Ile

50 55 60

Asp Val Val Val Phe Asp Gly Val Glu Pro Asn Pro Lys Asp Thr Asn

65 70 75 80

Val Arg Asp Gly Leu Glu Val Phe Arg Lys Glu His Cys Asp Ile Ile

85 90 95

Val Thr Val Gly Gly Gly Ser Pro His Asp Cys Gly Lys Gly Ile Gly

100 105 110

Ile Ala Ala Thr His Glu Gly Asp Leu Tyr Ser Tyr Ala Gly Ile Glu

115 120 125

Thr Leu Thr Asn Pro Leu Pro Pro Ile Val Ala Val Asn Thr Thr Ala

130 135 140

Gly Thr Ala Ser Glu Val Thr Arg His Cys Val Leu Thr Asn Thr Lys

145 150 155 160

Thr Lys Val Lys Phe Val Ile Val Ser Trp Arg Asn Leu Pro Ser Val

165 170 175

Ser Ile Asn Asp Pro Leu Leu Met Leu Gly Lys Pro Ala Pro Leu Thr

180 185 190

Ala Ala Thr Gly Met Asp Ala Leu Thr His Ala Val Glu Ala Tyr Ile

195 200 205

Ser Lys Asp Ala Asn Pro Val Thr Asp Ala Ala Ala Ile Gln Ala Ile

210 215 220

Arg Leu Ile Ala Arg Asn Leu Arg Gln Ala Val Ala Leu Gly Ser Asn

225 230 235 240

Leu Lys Ala Arg Glu Asn Met Ala Tyr Ala Ser Leu Leu Ala Gly Met

245 250 255

Ala Phe Asn Asn Ala Asn Leu Gly Tyr Val His Ala Met Ala His Gln

260 265 270

Leu Gly Gly Leu Tyr Asp Met Pro His Gly Val Ala Asn Ala Val Leu

275 280 285

Leu Pro His Val Ala Arg Tyr Asn Leu Ile Ala Asn Pro Glu Lys Phe

290 295 300

Ala Asp Ile Ala Glu Phe Met Gly Glu Asn Thr Asp Gly Leu Ser Thr

305 310 315 320

Met Asp Ala Ala Glu Leu Ala Ile His Ala Ile Ala Arg Leu Ser Ala

325 330 335

Asp Ile Gly Ile Pro Gln His Leu Arg Asp Leu Gly Val Lys Glu Ala

340 345 350

Asp Phe Pro Tyr Met Ala Glu Met Ala Leu Lys Asp Gly Asn Ala Phe

355 360 365

Ser Asn Pro Arg Lys Gly Asn Glu Lys Glu Ile Ala Glu Ile Phe Arg

370 375 380

Gln Ala Phe

385

<210> 132

<211> 387

<212> PRT

<213> 肺炎克雷伯氏菌(klebsiella pneumoniae)

<400> 132

Met Ser Tyr Arg Met Phe Asp Tyr Leu Val Pro Asn Val Asn Phe Phe

1 5 10 15

Gly Pro Asn Ala Ile Ser Val Val Gly Glu Arg Cys Gln Leu Leu Gly

20 25 30

Gly Lys Lys Ala Leu Leu Val Thr Asp Lys Gly Leu Arg Ala Ile Lys

35 40 45

Asp Gly Ala Val Asp Lys Thr Leu His Tyr Leu Arg Glu Ala Gly Ile

50 55 60

Glu Val Ala Ile Phe Asp Gly Val Glu Pro Asn Pro Lys Asp Thr Asn

65 70 75 80

Val Arg Asp Gly Leu Ala Val Phe Arg Arg Glu Gln Cys Asp Ile Ile

85 90 95

Val Thr Val Gly Gly Gly Ser Pro His Asp Cys Gly Lys Gly Ile Gly

100 105 110

Ile Ala Ala Thr His Glu Gly Asp Leu Tyr Gln Tyr Ala Gly Ile Glu

115 120 125

Thr Leu Thr Asn Pro Leu Pro Pro Ile Val Ala Val Asn Thr Thr Ala

130 135 140

Gly Thr Ala Ser Glu Val Thr Arg His Cys Val Leu Thr Asn Thr Glu

145 150 155 160

Thr Lys Val Lys Phe Val Ile Val Ser Trp Arg Asn Leu Pro Ser Val

165 170 175

Ser Ile Asn Asp Pro Leu Leu Met Ile Gly Lys Pro Ala Ala Leu Thr

180 185 190

Ala Ala Thr Gly Met Asp Ala Leu Thr His Ala Val Glu Ala Tyr Ile

195 200 205

Ser Lys Asp Ala Asn Pro Val Thr Asp Ala Ala Ala Met Gln Ala Ile

210 215 220

Arg Leu Ile Ala Arg Asn Leu Arg Gln Ala Val Ala Leu Gly Ser Asn

225 230 235 240

Leu Gln Ala Arg Glu Asn Met Ala Tyr Ala Ser Leu Leu Ala Gly Met

245 250 255

Ala Phe Asn Asn Ala Asn Leu Gly Tyr Val His Ala Met Ala His Gln

260 265 270

Leu Gly Gly Leu Tyr Asp Met Pro His Gly Val Ala Asn Ala Val Leu

275 280 285

Leu Pro His Val Ala Arg Tyr Asn Leu Ile Ala Asn Pro Glu Lys Phe

290 295 300

Ala Asp Ile Ala Glu Leu Met Gly Glu Asn Ile Thr Gly Leu Ser Thr

305 310 315 320

Leu Asp Ala Ala Glu Lys Ala Ile Ala Ala Ile Thr Arg Leu Ser Met

325 330 335

Asp Ile Gly Ile Pro Gln His Leu Arg Asp Leu Gly Val Lys Glu Ala

340 345 350

Asp Phe Pro Tyr Met Ala Glu Met Ala Leu Lys Asp Gly Asn Ala Phe

355 360 365

Ser Asn Pro Arg Lys Gly Asn Glu Gln Glu Ile Ala Ala Ile Phe Arg

370 375 380

Gln Ala Phe

385

<210> 133

<211> 22

<212> PRT

<213> 红串红球菌(Rhodoccus erythropolis)

<400> 133

Met Lys Thr Lys Ala Ala Val Leu Phe Glu Thr His Lys Pro Phe Glu

1 5 10 15

Ile Val Glu Leu Glu Leu

20

<210> 134

<211> 371

<212> PRT

<213> 嗜甲基拟无枝酸菌(Amycolatopsis methanolica)

<400> 134

Met Lys Thr Lys Ala Ala Val Leu His Ser Ala Gly Lys Pro Phe Glu

1 5 10 15

Ile Glu Glu Leu Glu Leu Asp Gly Pro Arg Glu Gly Glu Val Leu Ile

20 25 30

Lys Tyr Thr Ala Ala Gly Leu Cys His Ser Asp Leu His Leu Ile Asp

35 40 45

Asn Asp Leu Val Pro Arg Phe Pro Ile Val Gly Gly His Glu Gly Ala

50 55 60

Gly Val Ile Glu Asp Val Gly Pro Gly Val Thr Lys Val Lys Pro Gly

65 70 75 80

Asp His Val Val Cys Ser Phe Ile Pro Asn Cys Gly Thr Cys Arg Tyr

85 90 95

Cys Ala Thr Gly Arg Ser Asn Leu Cys Asp Met Gly Ala Thr Ile Leu

100 105 110

Asp Gly Gly Met Pro Asp Gly Ser Phe Arg Phe His Arg Gly Gly Thr

115 120 125

Asp Tyr Gly Ala Met Cys Met Leu Gly Thr Phe Ser Glu Arg Ala Thr

130 135 140

Ile Ser Gln His Ser Val Val Lys Val Asp Asp Trp Leu Pro Leu Glu

145 150 155 160

Thr Ala Val Leu Val Gly Cys Gly Val Pro Thr Gly Trp Ala Ser Ala

165 170 175

Asn Tyr Ala Gly Gly Val Arg Ala Gly Asp Thr Cys Val Val Tyr Gly

180 185 190

Ile Gly Gly Ile Gly Ile Asn Ala Val Gln Gly Ala Ala His Ala Gly

195 200 205

Ala Ala Asn Val Ile Ala Val Asp Pro Val Ala Phe Lys Arg Glu Lys

210 215 220

Ala Leu Glu Leu Gly Ala Thr His Ala Phe Ala Ser Ala Asp Glu Ala

225 230 235 240

Ala Ala Lys Val Ala Glu Leu Thr Trp Gly Gln Met Ala Asp Gln Ala

245 250 255

Leu Ile Thr Val Gly Thr Val Val Glu Gln Val Val Thr Asp Ala Phe

260 265 270

Asn Val Ile Gly Lys Gly Gly Thr Val Val Ile Thr Gly Leu Ala Asn

275 280 285

Pro Glu Lys Leu Thr Val His Leu Ser Gly Gly Val Met Thr Leu Phe

290 295 300

Glu Lys Thr Val Lys Gly Thr Leu Phe Gly Ser Ala Asn Pro Gln Tyr

305 310 315 320

Asp Ile Val Arg Leu Leu Arg Leu Tyr Gln Ala Gly His Val Lys Leu

325 330 335

Asp Glu Leu Val Thr Lys Arg Tyr Ser Leu Glu Glu Val Asn Glu Gly

340 345 350

Tyr Gln Asp Leu Arg Asp Gly Lys Asn Ile Arg Gly Val Ile Met His

355 360 365

Ser Ala Asp

370

<210> 135

<211> 382

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 135

Met Ala Asn Arg Met Ile Leu Asn Glu Thr Ala Trp Phe Gly Arg Gly

1 5 10 15

Ala Val Gly Ala Leu Thr Asp Glu Val Lys Arg Arg Gly Tyr Gln Lys

20 25 30

Ala Leu Ile Val Thr Asp Lys Thr Leu Val Gln Cys Gly Val Val Ala

35 40 45

Lys Val Thr Asp Lys Met Asp Ala Ala Gly Leu Ala Trp Ala Ile Tyr

50 55 60

Asp Gly Val Val Pro Asn Pro Thr Ile Thr Val Val Lys Glu Gly Leu

65 70 75 80

Gly Val Phe Gln Asn Ser Gly Ala Asp Tyr Leu Ile Ala Ile Gly Gly

85 90 95

Gly Ser Pro Gln Asp Thr Cys Lys Ala Ile Gly Ile Ile Ser Asn Asn

100 105 110

Pro Glu Phe Ala Asp Val Arg Ser Leu Glu Gly Leu Ser Pro Thr Asn

115 120 125

Lys Pro Ser Val Pro Ile Leu Ala Ile Pro Thr Thr Ala Gly Thr Ala

130 135 140

Ala Glu Val Thr Ile Asn Tyr Val Ile Thr Asp Glu Glu Lys Arg Arg

145 150 155 160

Lys Phe Val Cys Val Asp Pro His Asp Ile Pro Gln Val Ala Phe Ile

165 170 175

Asp Ala Asp Met Met Asp Gly Met Pro Pro Ala Leu Lys Ala Ala Thr

180 185 190

Gly Val Asp Ala Leu Thr His Ala Ile Glu Gly Tyr Ile Thr Arg Gly

195 200 205

Ala Trp Ala Leu Thr Asp Ala Leu His Ile Lys Ala Ile Glu Ile Ile

210 215 220

Ala Gly Ala Leu Arg Gly Ser Val Ala Gly Asp Lys Asp Ala Gly Glu

225 230 235 240

Glu Met Ala Leu Gly Gln Tyr Val Ala Gly Met Gly Phe Ser Asn Val

245 250 255

Gly Leu Gly Leu Val His Gly Met Ala His Pro Leu Gly Ala Phe Tyr

260 265 270

Asn Thr Pro His Gly Val Ala Asn Ala Ile Leu Leu Pro His Val Met

275 280 285

Arg Tyr Asn Ala Asp Phe Thr Gly Glu Lys Tyr Arg Asp Ile Ala Arg

290 295 300

Val Met Gly Val Lys Val Glu Gly Met Ser Leu Glu Glu Ala Arg Asn

305 310 315 320

Ala Ala Val Glu Ala Val Phe Ala Leu Asn Arg Asp Val Gly Ile Pro

325 330 335

Pro His Leu Arg Asp Val Gly Val Arg Lys Glu Asp Ile Pro Ala Leu

340 345 350

Ala Gln Ala Ala Leu Asp Asp Val Cys Thr Gly Gly Asn Pro Arg Glu

355 360 365

Ala Thr Leu Glu Asp Ile Val Glu Leu Tyr His Thr Ala Trp

370 375 380

<210> 136

<211> 312

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 136

Met Asp Ile Ile Phe Tyr His Pro Thr Phe Asp Thr Gln Trp Trp Ile

1 5 10 15

Glu Ala Leu Arg Lys Ala Ile Pro Gln Ala Arg Val Arg Ala Trp Lys

20 25 30

Ser Gly Asp Asn Asp Ser Ala Asp Tyr Ala Leu Val Trp His Pro Pro

35 40 45

Val Glu Met Leu Ala Gly Arg Asp Leu Lys Ala Val Phe Ala Leu Gly

50 55 60

Ala Gly Val Asp Ser Ile Leu Ser Lys Leu Gln Ala His Pro Glu Met

65 70 75 80

Leu Asn Pro Ser Val Pro Leu Phe Arg Leu Glu Asp Thr Gly Met Gly

85 90 95

Glu Gln Met Gln Glu Tyr Ala Val Ser Gln Val Leu His Trp Phe Arg

100 105 110

Arg Phe Asp Asp Tyr Arg Ile Gln Gln Asn Ser Ser His Trp Gln Pro

115 120 125

Leu Pro Glu Tyr His Arg Glu Asp Phe Thr Ile Gly Ile Leu Gly Ala

130 135 140

Gly Val Leu Gly Ser Lys Val Ala Gln Ser Leu Gln Thr Trp Arg Phe

145 150 155 160

Pro Leu Arg Cys Trp Ser Arg Thr Arg Lys Ser Trp Pro Gly Val Gln

165 170 175

Ser Phe Ala Gly Arg Glu Glu Leu Ser Ala Phe Leu Ser Gln Cys Arg

180 185 190

Val Leu Ile Asn Leu Leu Pro Asn Thr Pro Glu Thr Val Gly Ile Ile

195 200 205

Asn Gln Gln Leu Leu Glu Lys Leu Pro Asp Gly Ala Tyr Leu Leu Asn

210 215 220

Leu Ala Arg Gly Val His Val Val Glu Asp Asp Leu Leu Ala Ala Leu

225 230 235 240

Asp Ser Gly Lys Val Lys Gly Ala Met Leu Asp Val Phe Asn Arg Glu

245 250 255

Pro Leu Pro Pro Glu Ser Pro Leu Trp Gln His Pro Arg Val Thr Ile

260 265 270

Thr Pro His Val Ala Ala Ile Thr Arg Pro Ala Glu Ala Val Glu Tyr

275 280 285

Ile Ser Arg Thr Ile Ala Gln Leu Glu Lys Gly Glu Arg Val Cys Gly

290 295 300

Gln Val Asp Arg Ala Arg Gly Tyr

305 310

<210> 137

<211> 324

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 137

Met Lys Pro Ser Val Ile Leu Tyr Lys Ala Leu Pro Asp Asp Leu Leu

1 5 10 15

Gln Arg Leu Gln Glu His Phe Thr Val His Gln Val Ala Asn Leu Ser

20 25 30

Pro Gln Thr Val Glu Gln Asn Ala Ala Ile Phe Ala Glu Ala Glu Gly

35 40 45

Leu Leu Gly Ser Asn Glu Asn Val Asn Ala Ala Leu Leu Glu Lys Met

50 55 60

Pro Lys Leu Arg Ala Thr Ser Thr Ile Ser Val Gly Tyr Asp Asn Phe

65 70 75 80

Asp Val Asp Ala Leu Thr Ala Arg Lys Ile Leu Leu Met His Thr Pro

85 90 95

Thr Val Leu Thr Glu Thr Val Ala Asp Thr Leu Met Ala Leu Val Leu

100 105 110

Ser Thr Ala Arg Arg Val Val Glu Val Ala Glu Arg Val Lys Ala Gly

115 120 125

Glu Trp Thr Ala Ser Ile Gly Pro Asp Trp Tyr Gly Thr Asp Val His

130 135 140

His Lys Thr Leu Gly Ile Val Gly Met Gly Arg Ile Gly Met Ala Leu

145 150 155 160

Ala Gln Arg Ala His Phe Gly Phe Asn Met Pro Ile Leu Tyr Asn Ala

165 170 175

Arg Arg His His Lys Glu Ala Glu Glu Arg Phe Asn Ala Arg Tyr Cys

180 185 190

Asp Leu Asp Thr Leu Leu Gln Glu Ser Asp Phe Val Cys Leu Ile Leu

195 200 205

Pro Leu Thr Asp Glu Thr His His Leu Phe Gly Ala Glu Gln Phe Ala

210 215 220

Lys Met Lys Ser Ser Ala Ile Phe Ile Asn Ala Gly Arg Gly Pro Val

225 230 235 240

Val Asp Glu Asn Ala Leu Ile Ala Ala Leu Gln Lys Gly Glu Ile His

245 250 255

Ala Ala Gly Leu Asp Val Phe Glu Gln Glu Pro Leu Ser Val Asp Ser

260 265 270

Pro Leu Leu Ser Met Ala Asn Val Val Ala Val Pro His Ile Gly Ser

275 280 285

Ala Thr His Glu Thr Arg Tyr Gly Met Ala Ala Cys Ala Val Asp Asn

290 295 300

Leu Ile Asp Ala Leu Gln Gly Lys Val Glu Lys Asn Cys Val Asn Pro

305 310 315 320

His Val Ala Asp

<210> 138

<211> 275

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 138

Met Ala Asn Pro Thr Val Ile Lys Leu Gln Asp Gly Asn Val Met Pro

1 5 10 15

Gln Leu Gly Leu Gly Val Trp Gln Ala Ser Asn Glu Glu Val Ile Thr

20 25 30

Ala Ile Gln Lys Ala Leu Glu Val Gly Tyr Arg Ser Ile Asp Thr Ala

35 40 45

Ala Ala Tyr Lys Asn Glu Glu Gly Val Gly Lys Ala Leu Lys Asn Ala

50 55 60

Ser Val Asn Arg Glu Glu Leu Phe Ile Thr Thr Lys Leu Trp Asn Asp

65 70 75 80

Asp His Lys Arg Pro Arg Glu Ala Leu Leu Asp Ser Leu Lys Lys Leu

85 90 95

Gln Leu Asp Tyr Ile Asp Leu Tyr Leu Met His Trp Pro Val Pro Ala

100 105 110

Ile Asp His Tyr Val Glu Ala Trp Lys Gly Met Ile Glu Leu Gln Lys

115 120 125

Glu Gly Leu Ile Lys Ser Ile Gly Val Cys Asn Phe Gln Ile His His

130 135 140

Leu Gln Arg Leu Ile Asp Glu Thr Gly Val Thr Pro Val Ile Asn Gln

145 150 155 160

Ile Glu Leu His Pro Leu Met Gln Gln Arg Gln Leu His Ala Trp Asn

165 170 175

Ala Thr His Lys Ile Gln Thr Glu Ser Trp Ser Pro Leu Ala Gln Gly

180 185 190

Gly Lys Gly Val Phe Asp Gln Lys Val Ile Arg Asp Leu Ala Asp Lys

195 200 205

Tyr Gly Lys Thr Pro Ala Gln Ile Val Ile Arg Trp His Leu Asp Ser

210 215 220

Gly Leu Val Val Ile Pro Lys Ser Val Thr Pro Ser Arg Ile Ala Glu

225 230 235 240

Asn Phe Asp Val Trp Asp Phe Arg Leu Asp Lys Asp Glu Leu Gly Glu

245 250 255

Ile Ala Lys Leu Asp Gln Gly Lys Arg Leu Gly Pro Asp Pro Asp Gln

260 265 270

Phe Gly Gly

275

<210> 139

<211> 267

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 139

Met Ala Ile Pro Ala Phe Gly Leu Gly Thr Phe Arg Leu Lys Asp Asp

1 5 10 15

Val Val Ile Ser Ser Val Ile Thr Ala Leu Glu Leu Gly Tyr Arg Ala

20 25 30

Ile Asp Thr Ala Gln Ile Tyr Asp Asn Glu Ala Ala Val Gly Gln Ala

35 40 45

Ile Ala Glu Ser Gly Val Pro Arg His Glu Leu Tyr Ile Thr Thr Lys

50 55 60

Ile Trp Ile Glu Asn Leu Ser Lys Asp Lys Leu Ile Pro Ser Leu Lys

65 70 75 80

Glu Ser Leu Gln Lys Leu Arg Thr Asp Tyr Val Asp Leu Thr Leu Ile

85 90 95

His Trp Pro Ser Pro Asn Asp Glu Val Ser Val Glu Glu Phe Met Gln

100 105 110

Ala Leu Leu Glu Ala Lys Lys Gln Gly Leu Thr Arg Glu Ile Gly Ile

115 120 125

Ser Asn Phe Thr Ile Pro Leu Met Glu Lys Ala Ile Ala Ala Val Gly

130 135 140

Ala Glu Asn Ile Ala Thr Asn Gln Ile Glu Leu Ser Pro Tyr Leu Gln

145 150 155 160

Asn Arg Lys Val Val Ala Trp Ala Lys Gln His Gly Ile His Ile Thr

165 170 175

Ser Tyr Met Thr Leu Ala Tyr Gly Lys Ala Leu Lys Asp Glu Val Ile

180 185 190

Ala Arg Ile Ala Ala Lys His Asn Ala Thr Pro Ala Gln Val Ile Leu

195 200 205

Ala Trp Ala Met Gly Glu Gly Tyr Ser Val Ile Pro Ser Ser Thr Lys

210 215 220

Arg Lys Asn Leu Glu Ser Asn Leu Lys Ala Gln Asn Leu Gln Leu Asp

225 230 235 240

Ala Glu Asp Lys Lys Ala Ile Ala Ala Leu Asp Cys Asn Asp Arg Leu

245 250 255

Val Ser Pro Glu Gly Leu Ala Pro Glu Trp Asp

260 265

<210> 140

<211> 495

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 140

Met Asn Phe His His Leu Ala Tyr Trp Gln Asp Lys Ala Leu Ser Leu

1 5 10 15

Ala Ile Glu Asn Arg Leu Phe Ile Asn Gly Glu Tyr Thr Ala Ala Ala

20 25 30

Glu Asn Glu Thr Phe Glu Thr Val Asp Pro Val Thr Gln Ala Pro Leu

35 40 45

Ala Lys Ile Ala Arg Gly Lys Ser Val Asp Ile Asp Arg Ala Met Ser

50 55 60

Ala Ala Arg Gly Val Phe Glu Arg Gly Asp Trp Ser Leu Ser Ser Pro

65 70 75 80

Ala Lys Arg Lys Ala Val Leu Asn Lys Leu Ala Asp Leu Met Glu Ala

85 90 95

His Ala Glu Glu Leu Ala Leu Leu Glu Thr Leu Asp Thr Gly Lys Pro

100 105 110

Ile Arg His Ser Leu Arg Asp Asp Ile Pro Gly Ala Ala Arg Ala Ile

115 120 125

Arg Trp Tyr Ala Glu Ala Ile Asp Lys Val Tyr Gly Glu Val Ala Thr

130 135 140

Thr Ser Ser His Glu Leu Ala Met Ile Val Arg Glu Pro Val Gly Val

145 150 155 160

Ile Ala Ala Ile Val Pro Trp Asn Phe Pro Leu Leu Leu Thr Cys Trp

165 170 175

Lys Leu Gly Pro Ala Leu Ala Ala Gly Asn Ser Val Ile Leu Lys Pro

180 185 190

Ser Glu Lys Ser Pro Leu Ser Ala Ile Arg Leu Ala Gly Leu Ala Lys

195 200 205

Glu Ala Gly Leu Pro Asp Gly Val Leu Asn Val Val Thr Gly Phe Gly

210 215 220

His Glu Ala Gly Gln Ala Leu Ser Arg His Asn Asp Ile Asp Ala Ile

225 230 235 240

Ala Phe Thr Gly Ser Thr Arg Thr Gly Lys Gln Leu Leu Lys Asp Ala

245 250 255

Gly Asp Ser Asn Met Lys Arg Val Trp Leu Glu Ala Gly Gly Lys Ser

260 265 270

Ala Asn Ile Val Phe Ala Asp Cys Pro Asp Leu Gln Gln Ala Ala Ser

275 280 285

Ala Thr Ala Ala Gly Ile Phe Tyr Asn Gln Gly Gln Val Cys Ile Ala

290 295 300

Gly Thr Arg Leu Leu Leu Glu Glu Ser Ile Ala Asp Glu Phe Leu Ala

305 310 315 320

Leu Leu Lys Gln Gln Ala Gln Asn Trp Gln Pro Gly His Pro Leu Asp

325 330 335

Pro Ala Thr Thr Met Gly Thr Leu Ile Asp Cys Ala His Ala Asp Ser

340 345 350

Val His Ser Phe Ile Arg Glu Gly Glu Ser Lys Gly Gln Leu Leu Leu

355 360 365

Asp Gly Arg Asn Ala Gly Leu Ala Ala Ala Ile Gly Pro Thr Ile Phe

370 375 380

Val Asp Val Asp Pro Asn Ala Ser Leu Ser Arg Glu Glu Ile Phe Gly

385 390 395 400

Pro Val Leu Val Val Thr Arg Phe Thr Ser Glu Glu Gln Ala Leu Gln

405 410 415

Leu Ala Asn Asp Ser Gln Tyr Gly Leu Gly Ala Ala Val Trp Thr Arg

420 425 430

Asp Leu Ser Arg Ala His Arg Met Ser Arg Arg Leu Lys Ala Gly Ser

435 440 445

Val Phe Val Asn Asn Tyr Asn Asp Gly Asp Met Thr Val Pro Phe Gly

450 455 460

Gly Tyr Lys Gln Ser Gly Asn Gly Arg Asp Lys Ser Leu His Ala Leu

465 470 475 480

Glu Lys Phe Thr Glu Leu Lys Thr Ile Trp Ile Ser Leu Glu Ala

485 490 495

<210> 141

<211> 496

<212> PRT

<213> 肺炎克雷伯氏菌(Klebsiella pneumoniae)

<400> 141

Met Met Asn Phe Gln His Leu Ala Tyr Trp Gln Glu Lys Ala Lys Asn

1 5 10 15

Leu Ala Ile Glu Thr Arg Leu Phe Ile Asn Gly Glu Tyr Cys Ala Ala

20 25 30

Ala Asp Asn Thr Thr Phe Glu Thr Ile Asp Pro Ala Ala Gln Gln Thr

35 40 45

Leu Ala Gln Val Ala Arg Gly Lys Lys Ala Asp Val Glu Arg Ala Val

50 55 60

Lys Ala Ala Arg Gln Ala Phe Asp Asn Gly Asp Trp Ser Gln Ala Ser

65 70 75 80

Pro Ala Gln Arg Lys Ala Ile Leu Thr Arg Phe Ala Asn Leu Met Glu

85 90 95

Ala His Arg Glu Glu Leu Ala Leu Leu Glu Thr Leu Asp Thr Gly Lys

100 105 110

Pro Ile Arg His Ser Leu Arg Asp Asp Ile Pro Gly Ala Ala Arg Ala

115 120 125

Ile Arg Trp Tyr Ala Glu Ala Leu Asp Lys Val Tyr Gly Glu Val Ala

130 135 140

Pro Thr Gly Ser Asn Glu Leu Ala Met Ile Val Arg Glu Pro Ile Gly

145 150 155 160

Val Ile Ala Ala Val Val Pro Trp Asn Phe Pro Leu Leu Leu Ala Cys

165 170 175

Trp Lys Leu Gly Pro Ala Leu Ala Ala Gly Asn Ser Val Ile Leu Lys

180 185 190

Pro Ser Glu Lys Ser Pro Leu Thr Ala Leu Arg Leu Ala Gly Leu Ala

195 200 205

Lys Glu Ala Gly Leu Pro Asp Gly Val Leu Asn Val Val Ser Gly Phe

210 215 220

Gly His Glu Ala Gly Gln Ala Leu Ala Leu His Pro Asp Val Glu Val

225 230 235 240

Ile Thr Phe Thr Gly Ser Thr Arg Thr Gly Lys Gln Leu Leu Lys Asp

245 250 255

Ala Gly Asp Ser Asn Met Lys Arg Val Trp Leu Glu Ala Gly Gly Lys

260 265 270

Ser Ala Asn Ile Val Phe Ala Asp Cys Pro Asp Leu Gln Gln Ala Val

275 280 285

Arg Ala Thr Ala Gly Gly Ile Phe Tyr Asn Gln Gly Gln Val Cys Ile

290 295 300

Ala Gly Thr Arg Leu Leu Leu Glu Glu Ser Ile Ala Asp Glu Phe Leu

305 310 315 320

Ala Arg Leu Lys Ala Glu Ala Gln His Trp Gln Pro Gly Asn Pro Leu

325 330 335

Asp Pro Asp Thr Thr Met Gly Met Leu Ile Asp Asn Thr His Ala Asp

340 345 350

Asn Val His Ser Phe Ile Arg Gly Gly Glu Ser Gln Ser Thr Leu Phe

355 360 365

Leu Asp Gly Arg Lys Asn Pro Trp Pro Ala Ala Val Gly Pro Thr Ile

370 375 380

Phe Val Asp Val Asp Pro Ala Ser Thr Leu Ser Arg Glu Glu Ile Phe

385 390 395 400

Gly Pro Val Leu Val Val Thr Arg Phe Lys Ser Glu Glu Glu Ala Leu

405 410 415

Lys Leu Ala Asn Asp Ser Asp Tyr Gly Leu Gly Ala Ala Val Trp Thr

420 425 430

Arg Asp Leu Ser Arg Ala His Arg Met Ser Arg Arg Leu Lys Ala Gly

435 440 445

Ser Val Phe Val Asn Asn Tyr Asn Asp Gly Asp Met Thr Val Pro Phe

450 455 460

Gly Gly Tyr Lys Gln Ser Gly Asn Gly Arg Asp Lys Ser Leu His Ala

465 470 475 480

Leu Glu Lys Phe Thr Glu Leu Lys Thr Ile Trp Ile Ala Leu Glu Ser

485 490 495

<210> 142

<211> 519

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 142

Met Phe Ser Arg Ser Thr Leu Cys Leu Lys Thr Ser Ala Ser Ser Ile

1 5 10 15

Gly Arg Leu Gln Leu Arg Tyr Phe Ser His Leu Pro Met Thr Val Pro

20 25 30

Ile Lys Leu Pro Asn Gly Leu Glu Tyr Glu Gln Pro Thr Gly Leu Phe

35 40 45

Ile Asn Asn Lys Phe Val Pro Ser Lys Gln Asn Lys Thr Phe Glu Val

50 55 60

Ile Asn Pro Ser Thr Glu Glu Glu Ile Cys His Ile Tyr Glu Gly Arg

65 70 75 80

Glu Asp Asp Val Glu Glu Ala Val Gln Ala Ala Asp Arg Ala Phe Ser

85 90 95

Asn Gly Ser Trp Asn Gly Ile Asp Pro Ile Asp Arg Gly Lys Ala Leu

100 105 110

Tyr Arg Leu Ala Glu Leu Ile Glu Gln Asp Lys Asp Val Ile Ala Ser

115 120 125

Ile Glu Thr Leu Asp Asn Gly Lys Ala Ile Ser Ser Ser Arg Gly Asp

130 135 140

Val Asp Leu Val Ile Asn Tyr Leu Lys Ser Ser Ala Gly Phe Ala Asp

145 150 155 160

Lys Ile Asp Gly Arg Met Ile Asp Thr Gly Arg Thr His Phe Ser Tyr

165 170 175

Thr Lys Arg Gln Pro Leu Gly Val Cys Gly Gln Ile Ile Pro Trp Asn

180 185 190

Phe Pro Leu Leu Met Trp Ala Trp Lys Ile Ala Pro Ala Leu Val Thr

195 200 205

Gly Asn Thr Val Val Leu Lys Thr Ala Glu Ser Thr Pro Leu Ser Ala

210 215 220

Leu Tyr Val Ser Lys Tyr Ile Pro Gln Ala Gly Ile Pro Pro Gly Val

225 230 235 240

Ile Asn Ile Val Ser Gly Phe Gly Lys Ile Val Gly Glu Ala Ile Thr

245 250 255

Asn His Pro Lys Ile Lys Lys Val Ala Phe Thr Gly Ser Thr Ala Thr

260 265 270

Gly Arg His Ile Tyr Gln Ser Ala Ala Ala Gly Leu Lys Lys Val Thr

275 280 285

Leu Glu Leu Gly Gly Lys Ser Pro Asn Ile Val Phe Ala Asp Ala Glu

290 295 300

Leu Lys Lys Ala Val Gln Asn Ile Ile Leu Gly Ile Tyr Tyr Asn Ser

305 310 315 320

Gly Glu Val Cys Cys Ala Gly Ser Arg Val Tyr Val Glu Glu Ser Ile

325 330 335

Tyr Asp Lys Phe Ile Glu Glu Phe Lys Ala Ala Ser Glu Ser Ile Lys

340 345 350

Val Gly Asp Pro Phe Asp Glu Ser Thr Phe Gln Gly Ala Gln Thr Ser

355 360 365

Gln Met Gln Leu Asn Lys Ile Leu Lys Tyr Val Asp Ile Gly Lys Asn

370 375 380

Glu Gly Ala Thr Leu Ile Thr Gly Gly Glu Arg Leu Gly Ser Lys Gly

385 390 395 400

Tyr Phe Ile Lys Pro Thr Val Phe Gly Asp Val Lys Glu Asp Met Arg

405 410 415

Ile Val Lys Glu Glu Ile Phe Gly Pro Val Val Thr Val Thr Lys Phe

420 425 430

Lys Ser Ala Asp Glu Val Ile Asn Met Ala Asn Asp Ser Glu Tyr Gly

435 440 445

Leu Ala Ala Gly Ile His Thr Ser Asn Ile Asn Thr Ala Leu Lys Val

450 455 460

Ala Asp Arg Val Asn Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asp

465 470 475 480

Phe His His Ala Val Pro Phe Gly Gly Phe Asn Ala Ser Gly Leu Gly

485 490 495

Arg Glu Met Ser Val Asp Ala Leu Gln Asn Tyr Leu Gln Val Lys Ala

500 505 510

Val Arg Ala Lys Leu Asp Glu

515

<210> 143

<211> 520

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 143

Met Leu Ser Arg Thr Arg Ala Ala Ala Pro Asn Ser Arg Ile Phe Thr

1 5 10 15

Arg Ser Leu Leu Arg Leu Tyr Ser Gln Ala Pro Leu Arg Val Pro Ile

20 25 30

Thr Leu Pro Asn Gly Phe Thr Tyr Glu Gln Pro Thr Gly Leu Phe Ile

35 40 45

Asn Gly Glu Phe Val Ala Ser Lys Gln Lys Lys Thr Phe Asp Val Ile

50 55 60

Asn Pro Ser Asn Glu Glu Lys Ile Thr Thr Val Tyr Lys Ala Met Glu

65 70 75 80

Asp Asp Val Asp Glu Ala Val Ala Ala Ala Lys Lys Ala Phe Glu Thr

85 90 95

Lys Trp Ser Ile Val Glu Pro Glu Val Arg Ala Lys Ala Leu Phe Asn

100 105 110

Leu Ala Asp Leu Val Glu Lys His Gln Glu Thr Leu Ala Ala Ile Glu

115 120 125

Ser Met Asp Asn Gly Lys Ser Leu Phe Cys Ala Arg Gly Asp Val Ala

130 135 140

Leu Val Ser Lys Tyr Leu Arg Ser Cys Gly Gly Trp Ala Asp Lys Ile

145 150 155 160

Tyr Gly Asn Val Ile Asp Thr Gly Lys Asn His Phe Thr Tyr Ser Ile

165 170 175

Lys Glu Pro Leu Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro

180 185 190

Leu Leu Met Trp Ser Trp Lys Ile Gly Pro Ala Leu Ala Thr Gly Asn

195 200 205

Thr Val Val Leu Lys Pro Ala Glu Thr Thr Pro Leu Ser Ala Leu Phe

210 215 220

Ala Ser Gln Leu Cys Gln Glu Ala Gly Ile Pro Ala Gly Val Val Asn

225 230 235 240

Ile Leu Pro Gly Ser Gly Arg Val Val Gly Glu Arg Leu Ser Ala His

245 250 255

Pro Asp Val Lys Lys Ile Ala Phe Thr Gly Ser Thr Ala Thr Gly Arg

260 265 270

His Ile Met Lys Val Ala Ala Asp Thr Val Lys Lys Val Thr Leu Glu

275 280 285

Leu Gly Gly Lys Ser Pro Asn Ile Val Phe Ala Asp Ala Asp Leu Asp

290 295 300

Lys Ala Val Lys Asn Ile Ala Phe Gly Ile Phe Tyr Asn Ser Gly Glu

305 310 315 320

Val Cys Cys Ala Gly Ser Arg Ile Tyr Ile Gln Asp Thr Val Tyr Glu

325 330 335

Glu Val Leu Gln Lys Leu Lys Asp Tyr Thr Glu Ser Leu Lys Val Gly

340 345 350

Asp Pro Phe Asp Glu Glu Val Phe Gln Gly Ala Gln Thr Ser Asp Lys

355 360 365

Gln Leu His Lys Ile Leu Asp Tyr Val Asp Val Ala Lys Ser Glu Gly

370 375 380

Ala Arg Leu Val Thr Gly Gly Ala Arg His Gly Ser Lys Gly Tyr Phe

385 390 395 400

Val Lys Pro Thr Val Phe Ala Asp Val Lys Glu Asp Met Arg Ile Val

405 410 415

Lys Glu Glu Val Phe Gly Pro Ile Val Thr Val Ser Lys Phe Ser Thr

420 425 430

Val Asp Glu Val Ile Ala Met Ala Asn Asp Ser Gln Tyr Gly Leu Ala

435 440 445

Ala Gly Ile His Thr Asn Asp Ile Asn Lys Ala Val Asp Val Ser Lys

450 455 460

Arg Val Lys Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asn Phe His

465 470 475 480

Gln Asn Val Pro Phe Gly Gly Phe Gly Gln Ser Gly Ile Gly Arg Glu

485 490 495

Met Gly Glu Ala Ala Leu Ser Asn Tyr Thr Gln Thr Lys Ser Val Arg

500 505 510

Ile Ala Ile Asp Lys Pro Ile Arg

515 520

<210> 144

<211> 462

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 144

Met Thr Ile Thr Pro Ala Thr His Ala Ile Ser Ile Asn Pro Ala Thr

1 5 10 15

Gly Glu Gln Leu Ser Val Leu Pro Trp Ala Gly Ala Asp Asp Ile Glu

20 25 30

Asn Ala Leu Gln Leu Ala Ala Ala Gly Phe Arg Asp Trp Arg Glu Thr

35 40 45

Asn Ile Asp Tyr Arg Ala Glu Lys Leu Arg Asp Ile Gly Lys Ala Leu

50 55 60

Arg Ala Arg Ser Glu Glu Met Ala Gln Met Ile Thr Arg Glu Met Gly

65 70 75 80

Lys Pro Ile Asn Gln Ala Arg Ala Glu Val Ala Lys Ser Ala Asn Leu

85 90 95

Cys Asp Trp Tyr Ala Glu His Gly Pro Ala Met Leu Lys Ala Glu Pro

100 105 110

Thr Leu Val Glu Asn Gln Gln Ala Val Ile Glu Tyr Arg Pro Leu Gly

115 120 125

Thr Ile Leu Ala Ile Met Pro Trp Asn Phe Pro Leu Trp Gln Val Met

130 135 140

Arg Gly Ala Val Pro Ile Ile Leu Ala Gly Asn Gly Tyr Leu Leu Lys

145 150 155 160

His Ala Pro Asn Val Met Gly Cys Ala Gln Leu Ile Ala Gln Val Phe

165 170 175

Lys Asp Ala Gly Ile Pro Gln Gly Val Tyr Gly Trp Leu Asn Ala Asp

180 185 190

Asn Asp Gly Val Ser Gln Met Ile Lys Asp Ser Arg Ile Ala Ala Val

195 200 205

Thr Val Thr Gly Ser Val Arg Ala Gly Ala Ala Ile Gly Ala Gln Ala

210 215 220

Gly Ala Ala Leu Lys Lys Cys Val Leu Glu Leu Gly Gly Ser Asp Pro

225 230 235 240

Phe Ile Val Leu Asn Asp Ala Asp Leu Glu Leu Ala Val Lys Ala Ala

245 250 255

Val Ala Gly Arg Tyr Gln Asn Thr Gly Gln Val Cys Ala Ala Ala Lys

260 265 270

Arg Phe Ile Ile Glu Glu Gly Ile Ala Ser Ala Phe Thr Glu Arg Phe

275 280 285

Val Ala Ala Ala Ala Ala Leu Lys Met Gly Asp Pro Arg Asp Glu Glu

290 295 300

Asn Ala Leu Gly Pro Met Ala Arg Phe Asp Leu Arg Asp Glu Leu His

305 310 315 320

His Gln Val Glu Lys Thr Leu Ala Gln Gly Ala Arg Leu Leu Leu Gly

325 330 335

Gly Glu Lys Met Ala Gly Ala Gly Asn Tyr Tyr Pro Pro Thr Val Leu

340 345 350

Ala Asn Val Thr Pro Glu Met Thr Ala Phe Arg Glu Glu Met Phe Gly

355 360 365

Pro Val Ala Ala Ile Thr Ile Ala Lys Asp Ala Glu His Ala Leu Glu

370 375 380

Leu Ala Asn Asp Ser Glu Phe Gly Leu Ser Ala Thr Ile Phe Thr Thr

385 390 395 400

Asp Glu Thr Gln Ala Arg Gln Met Ala Ala Arg Leu Glu Cys Gly Gly

405 410 415

Val Phe Ile Asn Gly Tyr Cys Ala Ser Asp Ala Arg Val Ala Phe Gly

420 425 430

Gly Val Lys Lys Ser Gly Phe Gly Arg Glu Leu Ser His Phe Gly Leu

435 440 445

His Glu Phe Cys Asn Ile Gln Thr Val Trp Lys Asp Arg Ile

450 455 460

<210> 145

<211> 482

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 145

Met Lys Leu Asn Asp Ser Asn Leu Phe Arg Gln Gln Ala Leu Ile Asn

1 5 10 15

Gly Glu Trp Leu Asp Ala Asn Asn Gly Glu Ala Ile Asp Val Thr Asn

20 25 30

Pro Ala Asn Gly Asp Lys Leu Gly Ser Val Pro Lys Met Gly Ala Asp

35 40 45

Glu Thr Arg Ala Ala Ile Asp Ala Ala Asn Arg Ala Leu Pro Ala Trp

50 55 60

Arg Ala Leu Thr Ala Lys Glu Arg Ala Thr Ile Leu Arg Asn Trp Phe

65 70 75 80

Asn Leu Met Met Glu His Gln Asp Asp Leu Ala Arg Leu Met Thr Leu

85 90 95

Glu Gln Gly Lys Pro Leu Ala Glu Ala Lys Gly Glu Ile Ser Tyr Ala

100 105 110

Ala Ser Phe Ile Glu Trp Phe Ala Glu Glu Gly Lys Arg Ile Tyr Gly

115 120 125

Asp Thr Ile Pro Gly His Gln Ala Asp Lys Arg Leu Ile Val Ile Lys

130 135 140

Gln Pro Ile Gly Val Thr Ala Ala Ile Thr Pro Trp Asn Phe Pro Ala

145 150 155 160

Ala Met Ile Thr Arg Lys Ala Gly Pro Ala Leu Ala Ala Gly Cys Thr

165 170 175

Met Val Leu Lys Pro Ala Ser Gln Thr Pro Phe Ser Ala Leu Ala Leu

180 185 190

Ala Glu Leu Ala Ile Arg Ala Gly Val Pro Ala Gly Val Phe Asn Val

195 200 205

Val Thr Gly Ser Ala Gly Ala Val Gly Asn Glu Leu Thr Ser Asn Pro

210 215 220

Leu Val Arg Lys Leu Ser Phe Thr Gly Ser Thr Glu Ile Gly Arg Gln

225 230 235 240

Leu Met Glu Gln Cys Ala Lys Asp Ile Lys Lys Val Ser Leu Glu Leu

245 250 255

Gly Gly Asn Ala Pro Phe Ile Val Phe Asp Asp Ala Asp Leu Asp Lys

260 265 270

Ala Val Glu Gly Ala Leu Ala Ser Lys Phe Arg Asn Ala Gly Gln Thr

275 280 285

Cys Val Cys Ala Asn Arg Leu Tyr Val Gln Asp Gly Val Tyr Asp Arg

290 295 300

Phe Ala Glu Lys Leu Gln Gln Ala Val Ser Lys Leu His Ile Gly Asp

305 310 315 320

Gly Leu Asp Asn Gly Val Thr Ile Gly Pro Leu Ile Asp Glu Lys Ala

325 330 335

Val Ala Lys Val Glu Glu His Ile Ala Asp Ala Leu Glu Lys Gly Ala

340 345 350

Arg Val Val Cys Gly Gly Lys Ala His Glu Arg Gly Gly Asn Phe Phe

355 360 365

Gln Pro Thr Ile Leu Val Asp Val Pro Ala Asn Ala Lys Val Ser Lys

370 375 380

Glu Glu Thr Phe Gly Pro Leu Ala Pro Leu Phe Arg Phe Lys Asp Glu

385 390 395 400

Ala Asp Val Ile Ala Gln Ala Asn Asp Thr Glu Phe Gly Leu Ala Ala

405 410 415

Tyr Phe Tyr Ala Arg Asp Leu Ser Arg Val Phe Arg Val Gly Glu Ala

420 425 430

Leu Glu Tyr Gly Ile Val Gly Ile Asn Thr Gly Ile Ile Ser Asn Glu

435 440 445

Val Ala Pro Phe Gly Gly Ile Lys Ala Ser Gly Leu Gly Arg Glu Gly

450 455 460

Ser Lys Tyr Gly Ile Glu Asp Tyr Leu Glu Ile Lys Tyr Met Cys Ile

465 470 475 480

Gly Leu

<210> 146

<211> 474

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 146

Met Gln His Lys Leu Leu Ile Asn Gly Glu Leu Val Ser Gly Glu Gly

1 5 10 15

Glu Lys Gln Pro Val Tyr Asn Pro Ala Thr Gly Asp Val Leu Leu Glu

20 25 30

Ile Ala Glu Ala Ser Ala Glu Gln Val Asp Ala Ala Val Arg Ala Ala

35 40 45

Asp Ala Ala Phe Ala Glu Trp Gly Gln Thr Thr Pro Lys Val Arg Ala

50 55 60

Glu Cys Leu Leu Lys Leu Ala Asp Val Ile Glu Glu Asn Gly Gln Val

65 70 75 80

Phe Ala Glu Leu Glu Ser Arg Asn Cys Gly Lys Pro Leu His Ser Ala

85 90 95

Phe Asn Asp Glu Ile Pro Ala Ile Val Asp Val Phe Arg Phe Phe Ala

100 105 110

Gly Ala Ala Arg Cys Leu Asn Gly Leu Ala Ala Gly Glu Tyr Leu Glu

115 120 125

Gly His Thr Ser Met Ile Arg Arg Asp Pro Leu Gly Val Val Ala Ser

130 135 140

Ile Ala Pro Trp Asn Tyr Pro Leu Met Met Ala Ala Trp Lys Leu Ala

145 150 155 160

Pro Ala Leu Ala Ala Gly Asn Cys Val Val Leu Lys Pro Ser Glu Ile

165 170 175

Thr Pro Leu Thr Ala Leu Lys Leu Ala Glu Leu Ala Lys Asp Ile Phe

180 185 190

Pro Ala Gly Val Ile Asn Ile Leu Phe Gly Arg Gly Lys Thr Val Gly

195 200 205

Asp Pro Leu Thr Gly His Pro Lys Val Arg Met Val Ser Leu Thr Gly

210 215 220

Ser Ile Ala Thr Gly Glu His Ile Ile Ser His Thr Ala Ser Ser Ile

225 230 235 240

Lys Arg Thr His Met Glu Leu Gly Gly Lys Ala Pro Val Ile Val Phe

245 250 255

Asp Asp Ala Asp Ile Glu Ala Val Val Glu Gly Val Arg Thr Phe Gly

260 265 270

Tyr Tyr Asn Ala Gly Gln Asp Cys Thr Ala Ala Cys Arg Ile Tyr Ala

275 280 285

Gln Lys Gly Ile Tyr Asp Thr Leu Val Glu Lys Leu Gly Ala Ala Val

290 295 300

Ala Thr Leu Lys Ser Gly Ala Pro Asp Asp Glu Ser Thr Glu Leu Gly

305 310 315 320

Pro Leu Ser Ser Leu Ala His Leu Glu Arg Val Gly Lys Ala Val Glu

325 330 335

Glu Ala Lys Ala Thr Gly His Ile Lys Val Ile Thr Gly Gly Glu Lys

340 345 350

Arg Lys Gly Asn Gly Tyr Tyr Tyr Ala Pro Thr Leu Leu Ala Gly Ala

355 360 365

Leu Gln Asp Asp Ala Ile Val Gln Lys Glu Val Phe Gly Pro Val Val

370 375 380

Ser Val Thr Pro Phe Asp Asn Glu Glu Gln Val Val Asn Trp Ala Asn

385 390 395 400

Asp Ser Gln Tyr Gly Leu Ala Ser Ser Val Trp Thr Lys Asp Val Gly

405 410 415

Arg Ala His Arg Val Ser Ala Arg Leu Gln Tyr Gly Cys Thr Trp Val

420 425 430

Asn Thr His Phe Met Leu Val Ser Glu Met Pro His Gly Gly Gln Lys

435 440 445

Leu Ser Gly Tyr Gly Lys Asp Met Ser Leu Tyr Gly Leu Glu Asp Tyr

450 455 460

Thr Val Val Arg His Val Met Val Lys His

465 470

<210> 147

<211> 480

<212> PRT

<213> 恶臭假单胞菌(Pseudomonas putida)

<400> 147

Met Gln Leu Lys Asp Ala Gln Leu Phe Arg Gln Gln Ala Tyr Ile Asn

1 5 10 15

Gly Glu Trp Leu Asp Ala Asp Asn Gly Gln Thr Ile Lys Val Thr Asn

20 25 30

Pro Ala Thr Gly Glu Val Ile Gly Thr Val Pro Lys Met Gly Thr Ala

35 40 45

Glu Thr Arg Arg Ala Ile Glu Ala Ala Asp Lys Ala Leu Pro Ala Trp

50 55 60

Arg Ala Leu Thr Ala Lys Glu Arg Ser Ala Lys Leu Arg Arg Trp Phe

65 70 75 80

Glu Leu Met Ile Glu Asn Gln Asp Asp Leu Ala Arg Leu Met Thr Thr

85 90 95

Glu Gln Gly Lys Pro Leu Ala Glu Ala Lys Gly Glu Ile Ala Tyr Ala

100 105 110

Ala Ser Phe Ile Glu Trp Phe Ala Glu Glu Ala Lys Arg Ile Tyr Gly

115 120 125

Asp Thr Ile Pro Gly His Gln Pro Asp Lys Arg Leu Ile Val Ile Lys

130 135 140

Gln Pro Ile Gly Val Thr Ala Ala Ile Thr Pro Trp Asn Phe Pro Ala

145 150 155 160

Ala Met Ile Thr Arg Lys Ala Gly Pro Ala Leu Ala Ala Gly Cys Thr

165 170 175

Met Val Leu Lys Pro Ala Ser Gln Thr Pro Tyr Ser Ala Leu Ala Leu

180 185 190

Val Glu Leu Ala His Arg Ala Gly Ile Pro Ala Gly Val Leu Ser Val

195 200 205

Val Thr Gly Ser Ala Gly Glu Val Gly Gly Glu Leu Thr Gly Asn Ser

210 215 220

Leu Val Arg Lys Leu Ser Phe Thr Gly Ser Thr Glu Ile Gly Arg Gln

225 230 235 240

Leu Met Glu Glu Cys Ala Lys Asp Ile Lys Lys Val Ser Leu Glu Leu

245 250 255

Gly Gly Asn Ala Pro Phe Ile Val Phe Asp Asp Ala Asp Leu Asp Lys

260 265 270

Ala Val Glu Gly Ala Ile Ile Ser Lys Tyr Arg Asn Asn Gly Gln Thr

275 280 285

Cys Val Cys Ala Asn Arg Ile Tyr Val Gln Asp Gly Val Tyr Asp Ala

290 295 300

Phe Ala Glu Lys Leu Ala Ala Ala Val Ala Lys Leu Lys Ile Gly Asn

305 310 315 320

Gly Leu Glu Glu Gly Thr Thr Thr Gly Pro Leu Ile Asp Gly Lys Ala

325 330 335

Val Ala Lys Val Gln Glu His Ile Glu Asp Ala Val Ser Lys Gly Ala

340 345 350

Lys Val Leu Ser Gly Gly Lys Leu Ile Glu Gly Asn Phe Phe Glu Pro

355 360 365

Thr Ile Leu Val Asp Val Pro Lys Thr Ala Ala Val Ala Lys Glu Glu

370 375 380

Thr Phe Gly Pro Leu Ala Pro Leu Phe Arg Phe Lys Asp Glu Ala Glu

385 390 395 400

Val Ile Ala Met Ser Asn Asp Thr Glu Phe Gly Leu Ala Ser Tyr Phe

405 410 415

Tyr Ala Arg Asp Met Ser Arg Val Phe Arg Val Ala Glu Ala Leu Glu

420 425 430

Tyr Gly Met Val Gly Ile Asn Thr Gly Leu Ile Ser Asn Glu Val Ala

435 440 445

Pro Phe Gly Gly Ile Lys Ala Ser Gly Leu Gly Arg Glu Gly Ser Lys

450 455 460

Tyr Gly Ile Glu Asp Tyr Leu Glu Ile Lys Tyr Leu Cys Ile Ser Val

465 470 475 480

<210> 148

<211> 479

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 148

Met Ser Val Pro Val Gln His Pro Met Tyr Ile Asp Gly Gln Phe Val

1 5 10 15

Thr Trp Arg Gly Asp Ala Trp Ile Asp Val Val Asn Pro Ala Thr Glu

20 25 30

Ala Val Ile Ser Arg Ile Pro Asp Gly Gln Ala Glu Asp Ala Arg Lys

35 40 45

Ala Ile Asp Ala Ala Glu Arg Ala Gln Pro Glu Trp Glu Ala Leu Pro

50 55 60

Ala Ile Glu Arg Ala Ser Trp Leu Arg Lys Ile Ser Ala Gly Ile Arg

65 70 75 80

Glu Arg Ala Ser Glu Ile Ser Ala Leu Ile Val Glu Glu Gly Gly Lys

85 90 95

Ile Gln Gln Leu Ala Glu Val Glu Val Ala Phe Thr Ala Asp Tyr Ile

100 105 110

Asp Tyr Met Ala Glu Trp Ala Arg Arg Tyr Glu Gly Glu Ile Ile Gln

115 120 125

Ser Asp Arg Pro Gly Glu Asn Ile Leu Leu Phe Lys Arg Ala Leu Gly

130 135 140

Val Thr Thr Gly Ile Leu Pro Trp Asn Phe Pro Phe Phe Leu Ile Ala

145 150 155 160

Arg Lys Met Ala Pro Ala Leu Leu Thr Gly Asn Thr Ile Val Ile Lys

165 170 175

Pro Ser Glu Phe Thr Pro Asn Asn Ala Ile Ala Phe Ala Lys Ile Val

180 185 190

Asp Glu Ile Gly Leu Pro Arg Gly Val Phe Asn Leu Val Leu Gly Arg

195 200 205

Gly Glu Thr Val Gly Gln Glu Leu Ala Gly Asn Pro Lys Val Ala Met

210 215 220

Val Ser Met Thr Gly Ser Val Ser Ala Gly Glu Lys Ile Met Ala Thr

225 230 235 240

Ala Ala Lys Asn Ile Thr Lys Val Cys Leu Glu Leu Gly Gly Lys Ala

245 250 255

Pro Ala Ile Val Met Asp Asp Ala Asp Leu Glu Leu Ala Val Lys Ala

260 265 270

Ile Val Asp Ser Arg Val Ile Asn Ser Gly Gln Val Cys Asn Cys Ala

275 280 285

Glu Arg Val Tyr Val Gln Lys Gly Ile Tyr Asp Gln Phe Val Asn Arg

290 295 300

Leu Gly Glu Ala Met Gln Ala Val Gln Phe Gly Asn Pro Ala Glu Arg

305 310 315 320

Asn Asp Ile Ala Met Gly Pro Leu Ile Asn Ala Ala Ala Leu Glu Arg

325 330 335

Val Glu Gln Lys Val Ala Arg Ala Val Glu Glu Gly Ala Arg Val Ala

340 345 350

Phe Gly Gly Lys Ala Val Glu Gly Lys Gly Tyr Tyr Tyr Pro Pro Thr

355 360 365

Leu Leu Leu Asp Val Arg Gln Glu Met Ser Ile Met His Glu Glu Thr

370 375 380

Phe Gly Pro Val Leu Pro Val Val Ala Phe Asp Thr Leu Glu Asp Ala

385 390 395 400

Ile Ser Met Ala Asn Asp Ser Asp Tyr Gly Leu Thr Ser Ser Ile Tyr

405 410 415

Thr Gln Asn Leu Asn Val Ala Met Lys Ala Ile Lys Gly Leu Lys Phe

420 425 430

Gly Glu Thr Tyr Ile Asn Arg Glu Asn Phe Glu Ala Met Gln Gly Phe

435 440 445

His Ala Gly Trp Arg Lys Ser Gly Ile Gly Gly Ala Asp Gly Lys His

450 455 460

Gly Leu His Glu Tyr Leu Gln Thr Gln Val Val Tyr Leu Gln Ser

465 470 475

<210> 149

<211> 512

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 149

Met Thr Asn Asn Pro Pro Ser Ala Gln Ile Lys Pro Gly Glu Tyr Gly

1 5 10 15

Phe Pro Leu Lys Leu Lys Ala Arg Tyr Asp Asn Phe Ile Gly Gly Glu

20 25 30

Trp Val Ala Pro Ala Asp Gly Glu Tyr Tyr Gln Asn Leu Thr Pro Val

35 40 45

Thr Gly Gln Leu Leu Cys Glu Val Ala Ser Ser Gly Lys Arg Asp Ile

50 55 60

Asp Leu Ala Leu Asp Ala Ala His Lys Val Lys Asp Lys Trp Ala His

65 70 75 80

Thr Ser Val Gln Asp Arg Ala Ala Ile Leu Phe Lys Ile Ala Asp Arg

85 90 95

Met Glu Gln Asn Leu Glu Leu Leu Ala Thr Ala Glu Thr Trp Asp Asn

100 105 110

Gly Lys Pro Ile Arg Glu Thr Ser Ala Ala Asp Val Pro Leu Ala Ile

115 120 125

Asp His Phe Arg Tyr Phe Ala Ser Cys Ile Arg Ala Gln Glu Gly Gly

130 135 140

Ile Ser Glu Val Asp Ser Glu Thr Val Ala Tyr His Phe His Glu Pro

145 150 155 160

Leu Gly Val Val Gly Gln Ile Ile Pro Trp Asn Phe Pro Leu Leu Met

165 170 175

Ala Ser Trp Lys Met Ala Pro Ala Leu Ala Ala Gly Asn Cys Val Val

180 185 190

Leu Lys Pro Ala Arg Leu Thr Pro Leu Ser Val Leu Leu Leu Met Glu

195 200 205

Ile Val Gly Asp Leu Leu Pro Pro Gly Val Val Asn Val Val Asn Gly

210 215 220

Ala Gly Gly Val Ile Gly Glu Tyr Leu Ala Thr Ser Lys Arg Ile Ala

225 230 235 240

Lys Val Ala Phe Thr Gly Ser Thr Glu Val Gly Gln Gln Ile Met Gln

245 250 255

Tyr Ala Thr Gln Asn Ile Ile Pro Val Thr Leu Glu Leu Gly Gly Lys

260 265 270

Ser Pro Asn Ile Phe Phe Ala Asp Val Met Asp Glu Glu Asp Ala Phe

275 280 285

Phe Asp Lys Ala Leu Glu Gly Phe Ala Leu Phe Ala Phe Asn Gln Gly

290 295 300

Glu Val Cys Thr Cys Pro Ser Arg Ala Leu Val Gln Glu Ser Ile Tyr

305 310 315 320

Glu Arg Phe Met Glu Arg Ala Ile Arg Arg Val Glu Ser Ile Arg Ser

325 330 335

Gly Asn Pro Leu Asp Ser Val Thr Gln Met Gly Ala Gln Val Ser His

340 345 350

Gly Gln Leu Glu Thr Ile Leu Asn Tyr Ile Asp Ile Gly Lys Lys Glu

355 360 365

Gly Ala Asp Val Leu Thr Gly Gly Arg Arg Lys Leu Leu Glu Gly Glu

370 375 380

Leu Lys Asp Gly Tyr Tyr Leu Glu Pro Thr Ile Leu Phe Gly Gln Asn

385 390 395 400

Asn Met Arg Val Phe Gln Glu Glu Ile Phe Gly Pro Val Leu Ala Val

405 410 415

Thr Thr Phe Lys Thr Met Glu Glu Ala Leu Glu Leu Ala Asn Asp Thr

420 425 430

Gln Tyr Gly Leu Gly Ala Gly Val Trp Ser Arg Asn Gly Asn Leu Ala

435 440 445

Tyr Lys Met Gly Arg Gly Ile Gln Ala Gly Arg Val Trp Thr Asn Cys

450 455 460

Tyr His Ala Tyr Pro Ala His Ala Ala Phe Gly Gly Tyr Lys Gln Ser

465 470 475 480

Gly Ile Gly Arg Glu Thr His Lys Met Met Leu Glu His Tyr Gln Gln

485 490 495

Thr Lys Cys Leu Leu Val Ser Tyr Ser Asp Lys Pro Leu Gly Leu Phe

500 505 510

<210> 150

<211> 463

<212> PRT

<213> 詹氏甲烷球菌(Methanocaldococcus jannaschii)

<400> 150

Met Phe Ile Asp Gly Lys Trp Ile Asn Arg Glu Asp Met Asp Val Ile

1 5 10 15

Asn Pro Tyr Ser Leu Glu Val Ile Lys Lys Ile Pro Ala Leu Ser Arg

20 25 30

Glu Glu Ala Lys Glu Ala Ile Asp Thr Ala Glu Lys Tyr Lys Glu Val

35 40 45

Met Lys Asn Leu Pro Ile Thr Lys Arg Tyr Asn Ile Leu Met Asn Ile

50 55 60

Ala Lys Gln Ile Lys Glu Lys Lys Glu Glu Leu Ala Lys Ile Leu Ala

65 70 75 80

Ile Asp Ala Gly Lys Pro Ile Lys Gln Ala Arg Val Glu Val Glu Arg

85 90 95

Ser Ile Gly Thr Phe Lys Leu Ala Ala Phe Tyr Val Lys Glu His Arg

100 105 110

Asp Glu Val Ile Pro Ser Asp Asp Arg Leu Ile Phe Thr Arg Arg Glu

115 120 125

Pro Val Gly Ile Val Gly Ala Ile Thr Pro Phe Asn Phe Pro Leu Asn

130 135 140

Leu Ser Ala His Lys Ile Ala Pro Ala Ile Ala Thr Gly Asn Val Ile

145 150 155 160

Val His His Pro Ser Ser Lys Ala Pro Leu Val Cys Ile Glu Leu Ala

165 170 175

Lys Ile Ile Glu Asn Ala Leu Lys Lys Tyr Asn Val Pro Leu Gly Val

180 185 190

Tyr Asn Leu Leu Thr Gly Ala Gly Glu Val Val Gly Asp Glu Ile Val

195 200 205

Val Asn Glu Lys Val Asn Met Ile Ser Phe Thr Gly Ser Ser Lys Val

210 215 220

Gly Glu Leu Ile Thr Lys Lys Ala Gly Phe Lys Lys Ile Ala Leu Glu

225 230 235 240

Leu Gly Gly Val Asn Pro Asn Ile Val Leu Lys Asp Ala Asp Leu Asn

245 250 255

Lys Ala Val Asn Ala Leu Ile Lys Gly Ser Phe Ile Tyr Ala Gly Gln

260 265 270

Val Cys Ile Ser Val Gly Met Ile Leu Val Asp Glu Ser Ile Ala Asp

275 280 285

Lys Phe Ile Glu Met Phe Val Asn Lys Ala Lys Val Leu Asn Val Gly

290 295 300

Asn Pro Leu Asp Glu Lys Thr Asp Val Gly Pro Leu Ile Ser Val Glu

305 310 315 320

His Ala Glu Trp Val Glu Lys Val Val Glu Lys Ala Ile Asp Glu Gly

325 330 335

Gly Lys Leu Leu Leu Gly Gly Lys Arg Asp Lys Ala Leu Phe Tyr Pro

340 345 350

Thr Ile Leu Glu Val Asp Arg Asp Asn Ile Leu Cys Lys Thr Glu Thr

355 360 365

Phe Ala Pro Val Ile Pro Ile Ile Arg Thr Asn Glu Glu Glu Met Ile

370 375 380

Asp Ile Ala Asn Ser Thr Glu Tyr Gly Leu His Ser Ala Ile Phe Thr

385 390 395 400

Asn Asp Ile Asn Lys Ser Leu Lys Phe Ala Glu Asn Leu Glu Phe Gly

405 410 415

Gly Val Val Ile Asn Asp Ser Ser Leu Phe Arg Gln Asp Asn Met Pro

420 425 430

Phe Gly Gly Val Lys Lys Ser Gly Leu Gly Arg Glu Gly Val Lys Tyr

435 440 445

Ala Met Glu Glu Met Ser Asn Ile Lys Thr Ile Ile Ile Ser Lys

450 455 460

<210> 151

<211> 488

<212> PRT

<213> 地衣芽孢杆菌(Bacillus licheniformis)

<400> 151

Met Ser Val Ala Ala Glu Ser Lys Thr Tyr Phe Asn Phe Ile Asn Gly

1 5 10 15

Arg Trp Val Lys Ala Glu Ser Gly Gly Met Glu Gln Ser Leu Asn Pro

20 25 30

Ala Asp Thr Arg Asp Ile Val Gly Leu Val Gln Lys Ser Ser Ile Glu

35 40 45

Asp Val Asp Arg Ala Val Glu Ala Ala Lys Gln Ala Lys Lys Ala Trp

50 55 60

Arg Lys Leu Ser Gly Ala Glu Arg Gly Gln Phe Leu Tyr Lys Ala Ala

65 70 75 80

Asp Ile Met Glu Gln Arg Leu Asp Glu Ile Ala Glu Cys Ala Thr Arg

85 90 95

Glu Met Gly Lys Thr Leu Pro Glu Ala Lys Gly Glu Thr Ala Arg Gly

100 105 110

Ile Ala Ile Leu Arg Tyr Tyr Ala Gly Glu Gly Leu Arg Lys Thr Gly

115 120 125

Asp Val Ile Pro Ser Thr Asp Ser Ser Ala Phe Met Tyr Thr Asp Arg

130 135 140

Val Pro Leu Gly Val Val Gly Val Ile Ser Pro Trp Asn Phe Pro Val

145 150 155 160

Ala Ile Pro Ile Trp Lys Met Ala Pro Ala Leu Ile Tyr Gly Asn Thr

165 170 175

Val Val Ile Lys Pro Ala Thr Glu Thr Ala Val Thr Cys Leu Lys Val

180 185 190

Ile Ser Cys Phe Glu Glu Ala Gly Ile Pro Ser Gly Val Val Asn Ala

195 200 205

Val Thr Gly Pro Gly Ser Ser Ala Gly Gln Arg Leu Ala Glu His Pro

210 215 220

Asp Val Asn Gly Ile Thr Phe Thr Gly Ser Asn Gln Thr Gly Lys Ile

225 230 235 240

Ile Gly Arg Thr Ala Phe Glu Arg Gly Ala Lys Tyr Gln Leu Glu Met

245 250 255

Gly Gly Lys Asn Pro Val Ile Val Ala Asp Asp Ala Asp Leu Asp Ile

260 265 270

Ala Val Glu Ala Val Ile Ser Gly Ala Phe Arg Ser Thr Gly Gln Lys

275 280 285

Cys Thr Ala Thr Ser Arg Val Ile Val Leu Asn Gly Val Tyr Asp Arg

290 295 300

Phe Lys Glu Lys Leu Leu Gln Gln Thr Lys Glu Ile Thr Ile Gly Asp

305 310 315 320

Ser Leu Lys Glu Asp Val Trp Met Gly Pro Ile Ala Asn Lys Gln Gln

325 330 335

Leu Asp Asn Cys Leu Ser Tyr Ile Ala Lys Gly Lys Gln Glu Gly Ala

340 345 350

Asp Leu Ile Phe Gly Gly Glu Arg Leu Ala Asp Gly Lys Tyr Glu Asn

355 360 365

Gly Tyr Tyr Ile Arg Pro Ala Ile Phe Asp Asn Val Thr Ser Gly Met

370 375 380

Thr Ile Ala Gln Glu Glu Ile Phe Gly Pro Val Ile Ala Leu Ile Lys

385 390 395 400

Ala Asp Thr Leu Glu Glu Ala Leu Glu Thr Ala Asn Asp Val Lys Phe

405 410 415

Gly Leu Ser Ala Ser Ile Phe Thr Gln Asn Ile Arg Arg Met Leu Ser

420 425 430

Phe Thr Asp Glu Ile Glu Ala Gly Leu Ile Arg Val Asn Ala Glu Ser

435 440 445

Ala Gly Val Glu Leu Gln Ala Pro Phe Gly Gly Val Lys Gln Ser Ser

450 455 460

Ser His Ser Arg Glu Gln Gly Glu Ala Ala Lys Glu Phe Phe Thr Ala

465 470 475 480

Val Lys Thr Val Phe Val Lys Pro

485

<210> 152

<211> 491

<212> PRT

<213> 假单胞菌属菌种(Pseudomonas sp.)

<400> 152

Met Lys Gln Tyr Arg Asn Phe Val Asp Gly Lys Trp Val Glu Ser Ser

1 5 10 15

Lys Thr Phe Gln Asp Val Thr Pro Ile Asp Gly Ser Val Val Ala Val

20 25 30

Val His Glu Ala Asp Arg Asp Leu Val Asp Ala Ala Val Lys Ala Gly

35 40 45

His Arg Ala Leu Glu Gly Glu Trp Gly Arg Thr Thr Ala Ala Gln Arg

50 55 60

Val Asp Trp Leu Arg Arg Ile Ala Asn Glu Met Glu Arg Arg Gln Gln

65 70 75 80

Asp Phe Leu Asp Ala Glu Met Ala Asp Thr Gly Lys Pro Leu Ser Met

85 90 95

Ala Ala Thr Ile Asp Ile Pro Arg Gly Ile Ala Asn Phe Arg Asn Phe

100 105 110

Ala Asp Ile Leu Ala Thr Ala Pro Val Asp Ser His Arg Leu Asp Leu

115 120 125

Pro Asp Gly Ala Tyr Ala Leu Asn Tyr Ala Ala Arg Lys Pro Leu Gly

130 135 140

Val Val Gly Val Ile Ser Pro Trp Asn Leu Pro Leu Leu Leu Leu Thr

145 150 155 160

Trp Lys Val Ala Pro Ala Leu Ala Cys Gly Asn Ala Val Val Val Lys

165 170 175

Pro Ser Glu Asp Thr Pro Gly Thr Ala Thr Leu Leu Ala Glu Val Met

180 185 190

Glu Ala Val Gly Ile Pro Pro Gly Val Phe Asn Leu Val His Gly Phe

195 200 205

Gly Pro Asn Ser Ala Gly Glu Phe Ile Ser Gln His Pro Asp Ile Ser

210 215 220

Ala Ile Thr Phe Thr Gly Glu Ser Lys Thr Gly Ser Thr Ile Met Arg

225 230 235 240

Ala Ala Ala Glu Gly Val Lys Pro Val Ser Phe Glu Leu Gly Gly Lys

245 250 255

Asn Ala Ala Val Ile Phe Ala Asp Cys Asp Phe Glu Lys Met Leu Asp

260 265 270

Gly Met Met Arg Ala Leu Phe Leu Asn Ser Gly Gln Val Cys Leu Cys

275 280 285

Ser Glu Arg Val Tyr Val Glu Arg Pro Ile Phe Asp Arg Phe Cys Val

290 295 300

Ala Leu Ala Glu Arg Ile Lys Ala Leu Lys Val Asp Trp Pro His Glu

305 310 315 320

Thr Asp Thr Gln Met Gly Pro Leu Ile Ser Ser Lys His Arg Asp Lys

325 330 335

Val Leu Ser Tyr Phe Glu Leu Ala Arg Gln Glu Gly Ala Thr Phe Leu

340 345 350

Ala Gly Gly Gly Val Pro Arg Phe Gly Asp Glu Arg Asp Asn Gly Ala

355 360 365

Trp Val Glu Pro Thr Val Ile Ala Gly Leu Ser Asp Asp Ala Arg Val

370 375 380

Val Arg Glu Glu Ile Phe Gly Pro Ile Cys His Val Thr Pro Phe Asp

385 390 395 400

Ser Glu Ser Glu Val Ile Arg Arg Ala Asn Asp Thr Arg Tyr Gly Leu

405 410 415

Ala Ala Thr Ile Trp Thr Thr Asn Leu Ser Arg Ala His Arg Val Ser

420 425 430

Glu Leu Met Arg Val Gly Ile Ser Trp Val Asn Thr Trp Phe Leu Arg

435 440 445

Asp Leu Arg Thr Pro Phe Gly Gly Ala Gly Leu Ser Gly Ile Gly Arg

450 455 460

Glu Gly Gly Met His Ser Leu Asn Phe Tyr Ser Glu Leu Thr Asn Val

465 470 475 480

Cys Val Arg Ile Asp Lys Glu Ser Pro Asp Val

485 490

<210> 153

<211> 499

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 153

Met Thr Glu Pro His Val Ala Val Leu Ser Gln Val Gln Gln Phe Leu

1 5 10 15

Asp Arg Gln His Gly Leu Tyr Ile Asp Gly Arg Pro Gly Pro Ala Gln

20 25 30

Ser Glu Lys Arg Leu Ala Ile Phe Asp Pro Ala Thr Gly Gln Glu Ile

35 40 45

Ala Ser Thr Ala Asp Ala Asn Glu Ala Asp Val Asp Asn Ala Val Met

50 55 60

Ser Ala Trp Arg Ala Phe Val Ser Arg Arg Trp Ala Gly Arg Leu Pro

65 70 75 80

Ala Glu Arg Glu Arg Ile Leu Leu Arg Phe Ala Asp Leu Val Glu Gln

85 90 95

His Ser Glu Glu Leu Ala Gln Leu Glu Thr Leu Glu Gln Gly Lys Ser

100 105 110

Ile Ala Ile Ser Arg Ala Phe Glu Val Gly Cys Thr Leu Asn Trp Met

115 120 125

Arg Tyr Thr Ala Gly Leu Thr Thr Lys Ile Ala Gly Lys Thr Leu Asp

130 135 140

Leu Ser Ile Pro Leu Pro Gln Gly Ala Arg Tyr Gln Ala Trp Thr Arg

145 150 155 160

Lys Glu Pro Val Gly Val Val Ala Gly Ile Val Pro Trp Asn Phe Pro

165 170 175

Leu Met Ile Gly Met Trp Lys Val Met Pro Ala Leu Ala Ala Gly Cys

180 185 190

Ser Ile Val Ile Lys Pro Ser Glu Thr Thr Pro Leu Thr Met Leu Arg

195 200 205

Val Ala Glu Leu Ala Ser Glu Ala Gly Ile Pro Asp Gly Val Phe Asn

210 215 220

Val Val Thr Gly Ser Gly Ala Val Cys Gly Ala Ala Leu Thr Ser His

225 230 235 240

Pro His Val Ala Lys Ile Ser Phe Thr Gly Ser Thr Ala Thr Gly Lys

245 250 255

Gly Ile Ala Arg Thr Ala Ala Asp His Leu Thr Arg Val Thr Leu Glu

260 265 270

Leu Gly Gly Lys Asn Pro Ala Ile Val Leu Lys Asp Ala Asp Pro Gln

275 280 285

Trp Val Ile Glu Gly Leu Met Thr Gly Ser Phe Leu Asn Gln Gly Gln

290 295 300

Val Cys Ala Ala Ser Ser Arg Ile Tyr Ile Glu Ala Pro Leu Phe Asp

305 310 315 320

Thr Leu Val Ser Gly Phe Glu Gln Ala Val Lys Ser Leu Gln Val Gly

325 330 335

Pro Gly Met Ser Pro Val Ala Gln Ile Asn Pro Leu Val Ser Arg Ala

340 345 350

His Cys Asp Lys Val Cys Ser Phe Leu Asp Asp Ala Gln Ala Gln Gln

355 360 365

Ala Glu Leu Ile Arg Gly Ser Asn Gly Pro Ala Gly Glu Gly Tyr Tyr

370 375 380

Val Ala Pro Thr Leu Val Val Asn Pro Asp Ala Lys Leu Arg Leu Thr

385 390 395 400

Arg Glu Glu Val Phe Gly Pro Val Val Asn Leu Val Arg Val Ala Asp

405 410 415

Gly Glu Glu Ala Leu Gln Leu Ala Asn Asp Thr Glu Tyr Gly Leu Thr

420 425 430

Ala Ser Val Trp Thr Gln Asn Leu Ser Gln Ala Leu Glu Tyr Ser Asp

435 440 445

Arg Leu Gln Ala Gly Thr Val Trp Val Asn Ser His Thr Leu Ile Asp

450 455 460

Ala Asn Leu Pro Phe Gly Gly Met Lys Gln Ser Gly Thr Gly Arg Asp

465 470 475 480

Phe Gly Pro Asp Trp Leu Asp Gly Trp Cys Glu Thr Lys Ser Val Cys

485 490 495

Val Arg Tyr

<210> 154

<211> 492

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 154

Met Thr Leu Trp Ile Asn Gly Asp Trp Ile Thr Gly Gln Gly Ala Ser

1 5 10 15

Arg Val Lys Arg Asn Pro Val Ser Gly Glu Val Leu Trp Gln Gly Asn

20 25 30

Asp Ala Asp Ala Ala Gln Val Glu Gln Ala Cys Arg Ala Ala Arg Ala

35 40 45

Ala Phe Pro Arg Trp Ala Arg Leu Ser Phe Ala Glu Arg His Ala Val

50 55 60

Val Glu Arg Phe Ala Ala Leu Leu Glu Ser Asn Lys Ala Glu Leu Thr

65 70 75 80

Ala Ile Ile Ala Arg Glu Thr Gly Lys Pro Arg Trp Glu Ala Ala Thr

85 90 95

Glu Val Thr Ala Met Ile Asn Lys Ile Ala Ile Ser Ile Lys Ala Tyr

100 105 110

His Val Arg Thr Gly Glu Gln Arg Ser Glu Met Pro Asp Gly Ala Ala

115 120 125

Ser Leu Arg His Arg Pro His Gly Val Leu Ala Val Phe Gly Pro Tyr

130 135 140

Asn Phe Pro Gly His Leu Pro Asn Gly His Ile Val Pro Ala Leu Leu

145 150 155 160

Ala Gly Asn Thr Ile Ile Phe Lys Pro Ser Glu Leu Thr Pro Trp Ser

165 170 175

Gly Glu Ala Val Met Arg Leu Trp Gln Gln Ala Gly Leu Pro Pro Gly

180 185 190

Val Leu Asn Leu Val Gln Gly Gly Arg Glu Thr Gly Gln Ala Leu Ser

195 200 205

Ala Leu Glu Asp Leu Asp Gly Leu Leu Phe Thr Gly Ser Ala Asn Thr

210 215 220

Gly Tyr Gln Leu His Arg Gln Leu Ser Gly Gln Pro Glu Lys Ile Leu

225 230 235 240

Ala Leu Glu Met Gly Gly Asn Asn Pro Leu Ile Ile Asp Glu Val Ala

245 250 255

Asp Ile Asp Ala Ala Val His Leu Thr Ile Gln Ser Ala Phe Val Thr

260 265 270

Ala Gly Gln Arg Cys Thr Cys Ala Arg Arg Leu Leu Leu Lys Ser Gly

275 280 285

Ala Gln Gly Asp Ala Phe Leu Ala Arg Leu Val Ala Val Ser Gln Arg

290 295 300

Leu Thr Pro Gly Asn Trp Asp Asp Glu Pro Gln Pro Phe Ile Gly Gly

305 310 315 320

Leu Ile Ser Glu Gln Ala Ala Gln Gln Val Val Thr Ala Trp Gln Gln

325 330 335

Leu Glu Ala Met Gly Gly Arg Pro Leu Leu Ala Pro Arg Leu Leu Gln

340 345 350

Ala Gly Thr Ser Leu Leu Thr Pro Gly Ile Ile Glu Met Thr Gly Val

355 360 365

Ala Gly Val Pro Asp Glu Glu Val Phe Gly Pro Leu Leu Arg Val Trp

370 375 380

Arg Tyr Asp Thr Phe Asp Glu Ala Ile Arg Met Ala Asn Asn Thr Arg

385 390 395 400

Phe Gly Leu Ser Cys Gly Leu Val Ser Pro Glu Arg Glu Lys Phe Asp

405 410 415

Gln Leu Leu Leu Glu Ala Arg Ala Gly Ile Val Asn Trp Asn Lys Pro

420 425 430

Leu Thr Gly Ala Ala Ser Thr Ala Pro Phe Gly Gly Ile Gly Ala Ser

435 440 445

Gly Asn His Arg Pro Ser Ala Trp Tyr Ala Ala Asp Tyr Cys Ala Trp

450 455 460

Pro Met Ala Ser Leu Glu Ser Asp Ser Leu Thr Leu Pro Ala Thr Leu

465 470 475 480

Asn Pro Gly Leu Asp Phe Ser Asp Glu Val Val Arg

485 490

<210> 155

<211> 1358

<212> PRT

<213> 玉米(Zea mays (玉蜀黍(Maize))

<400> 155

Met Gly Lys Glu Ala Gly Ala Ala Glu Ser Ser Thr Val Val Leu Ala

1 5 10 15

Val Asn Gly Lys Arg Tyr Glu Ala Ala Gly Val Ala Pro Ser Thr Ser

20 25 30

Leu Leu Glu Phe Leu Arg Thr Gln Thr Pro Val Arg Gly Pro Lys Leu

35 40 45

Gly Cys Gly Glu Gly Gly Cys Gly Ala Cys Val Val Leu Val Ser Lys

50 55 60

Tyr Asp Pro Ala Thr Asp Glu Val Thr Glu Phe Ser Ala Ser Ser Cys

65 70 75 80

Leu Thr Leu Leu His Ser Val Asp Arg Cys Ser Val Thr Thr Ser Glu

85 90 95

Gly Ile Gly Asn Thr Arg Asp Gly Tyr His Pro Val Gln Gln Arg Leu

100 105 110

Ser Gly Phe His Ala Ser Gln Cys Gly Phe Cys Thr Pro Gly Met Cys

115 120 125

Met Ser Ile Phe Ser Ala Leu Val Lys Ala Asp Asn Lys Ser Asp Arg

130 135 140

Pro Asp Pro Pro Ala Gly Phe Ser Lys Ile Thr Thr Ser Glu Ala Glu

145 150 155 160

Lys Ala Val Ser Gly Asn Leu Cys Arg Cys Thr Gly Tyr Arg Pro Ile

165 170 175

Val Asp Thr Cys Lys Ser Phe Ala Ser Asp Val Asp Leu Glu Asp Leu

180 185 190

Gly Leu Asn Cys Phe Trp Lys Lys Gly Glu Glu Pro Ala Glu Val Ser

195 200 205

Arg Leu Pro Gly Tyr Asn Ser Gly Ala Val Cys Thr Phe Pro Glu Phe

210 215 220

Leu Lys Ser Glu Ile Lys Ser Thr Met Lys Gln Val Asn Asp Val Pro

225 230 235 240

Ile Ala Ala Ser Gly Asp Gly Trp Tyr His Pro Lys Ser Ile Glu Glu

245 250 255

Leu His Arg Leu Phe Asp Ser Ser Trp Phe Asp Asp Ser Ser Val Lys

260 265 270

Ile Val Ala Ser Asn Thr Gly Ser Gly Val Tyr Lys Asp Gln Asp Leu

275 280 285

Tyr Asp Lys Tyr Ile Asp Ile Lys Gly Ile Pro Glu Leu Ser Val Ile

290 295 300

Asn Lys Asn Asp Lys Ala Ile Glu Leu Gly Ser Val Val Ser Ile Ser

305 310 315 320

Lys Ala Ile Glu Val Leu Ser Asp Gly Asn Leu Val Phe Arg Lys Ile

325 330 335

Ala Asp His Leu Asn Lys Val Ala Ser Pro Phe Val Arg Asn Thr Ala

340 345 350

Thr Ile Gly Gly Asn Ile Met Met Ala Gln Arg Leu Pro Phe Glu Ser

355 360 365

Asp Val Ala Thr Val Leu Leu Ala Ala Gly Ser Thr Val Thr Val Gln

370 375 380

Val Ala Ser Lys Arg Leu Cys Phe Thr Leu Glu Glu Phe Leu Glu Gln

385 390 395 400

Pro Pro Cys Asp Ser Arg Thr Leu Leu Leu Ser Ile Phe Ile Pro Glu

405 410 415

Trp Gly Ser Asp Tyr Val Thr Phe Glu Thr Phe Arg Ala Ala Pro Arg

420 425 430

Pro Phe Gly Asn Ala Val Ser Tyr Val Asn Ser Ala Phe Leu Ala Arg

435 440 445

Thr Ser Gly Ser Leu Leu Ile Glu Asp Ile Cys Leu Ala Phe Gly Ala

450 455 460

Tyr Gly Val Asp His Ala Ile Arg Ala Lys Lys Val Glu Asp Phe Leu

465 470 475 480

Lys Gly Lys Ser Leu Ser Ser Phe Val Ile Leu Glu Ala Ile Lys Leu

485 490 495

Leu Lys Asp Thr Val Ser Pro Ser Glu Gly Thr Thr His His Glu Tyr

500 505 510

Arg Val Ser Leu Ala Val Ser Phe Leu Phe Ser Phe Leu Ser Ser Leu

515 520 525

Ala Asn Ser Ser Ser Ala Pro Ser Asn Ile Asp Thr Pro Asn Gly Ser

530 535 540

Tyr Thr His Glu Thr Gly Ser Asn Val Asp Ser Pro Glu Arg His Ile

545 550 555 560

Lys Val Asp Ser Asn Asp Leu Pro Ile Arg Ser Arg Gln Glu Met Val

565 570 575

Phe Ser Asp Glu Tyr Lys Pro Val Gly Lys Pro Ile Lys Lys Val Gly

580 585 590

Ala Glu Ile Gln Ala Ser Gly Glu Ala Val Tyr Val Asp Asp Ile Pro

595 600 605

Ala Pro Lys Asp Cys Leu Tyr Gly Ala Phe Ile Tyr Ser Thr His Pro

610 615 620

His Ala His Val Arg Ser Ile Asn Phe Lys Ser Ser Leu Ala Ser Gln

625 630 635 640

Lys Val Ile Thr Val Ile Thr Ala Lys Asp Ile Pro Ser Gly Gly Glu

645 650 655

Asn Ile Gly Ser Ser Phe Leu Met Gln Gly Glu Ala Leu Phe Ala Asp

660 665 670

Pro Ile Ala Glu Phe Ala Gly Gln Asn Ile Gly Val Val Ile Ala Glu

675 680 685

Thr Gln Arg Tyr Ala Asn Met Ala Ala Lys Gln Ala Val Val Glu Tyr

690 695 700

Ser Thr Glu Asn Leu Gln Pro Pro Ile Leu Thr Ile Glu Asp Ala Ile

705 710 715 720

Gln Arg Asn Ser Tyr Ile Gln Ile Pro Pro Phe Leu Ala Pro Lys Pro

725 730 735

Val Gly Asp Tyr Asn Lys Gly Met Ala Glu Ala Asp His Lys Ile Leu

740 745 750

Ser Ala Glu Val Lys Leu Glu Ser Gln Tyr Tyr Phe Tyr Met Glu Thr

755 760 765

Gln Ala Ala Leu Ala Ile Pro Asp Glu Asp Asn Cys Ile Thr Ile Tyr

770 775 780

Ser Ser Thr Gln Met Pro Glu Leu Thr Gln Asn Leu Ile Ala Arg Cys

785 790 795 800

Leu Gly Ile Pro Phe His Asn Val Arg Val Ile Ser Arg Arg Val Gly

805 810 815

Gly Gly Phe Gly Gly Lys Ala Met Lys Ala Thr His Thr Ala Cys Ala

820 825 830

Cys Ala Leu Ala Ala Phe Lys Leu Arg Arg Pro Val Arg Met Tyr Leu

835 840 845

Asp Arg Lys Thr Asp Met Ile Met Ala Gly Gly Arg His Pro Met Lys

850 855 860

Ala Lys Tyr Ser Val Gly Phe Lys Ser Asp Gly Lys Ile Thr Ala Leu

865 870 875 880

His Leu Asp Leu Gly Ile Asn Ala Gly Ile Ser Pro Asp Val Ser Pro

885 890 895

Leu Met Pro Arg Ala Ile Ile Gly Ala Leu Lys Lys Tyr Asn Trp Gly

900 905 910

Thr Leu Glu Phe Asp Thr Lys Val Cys Lys Thr Asn Val Ser Ser Lys

915 920 925

Ser Ala Met Arg Ala Pro Gly Asp Val Gln Gly Ser Phe Ile Ala Glu

930 935 940

Ala Ile Ile Glu His Val Ala Ser Ala Leu Ala Leu Asp Thr Asn Thr

945 950 955 960

Val Arg Arg Lys Asn Leu His Asp Phe Glu Ser Leu Glu Val Phe Tyr

965 970 975

Gly Glu Ser Ala Gly Glu Ala Ser Thr Tyr Ser Leu Val Ser Met Phe

980 985 990

Asp Lys Leu Ala Leu Ser Pro Glu Tyr Gln His Arg Ala Ala Met Ile

995 1000 1005

Glu Gln Phe Asn Ser Ser Asn Lys Trp Lys Lys Arg Gly Ile Ser

1010 1015 1020

Cys Val Pro Ala Thr Tyr Glu Val Asn Leu Arg Pro Thr Pro Gly

1025 1030 1035

Lys Val Ser Ile Met Asn Asp Gly Ser Ile Ala Val Glu Val Gly

1040 1045 1050

Gly Ile Glu Ile Gly Gln Gly Leu Trp Thr Lys Val Lys Gln Met

1055 1060 1065

Thr Ala Phe Gly Leu Gly Gln Leu Cys Pro Asp Gly Gly Glu Cys

1070 1075 1080

Leu Leu Asp Lys Val Arg Val Ile Gln Ala Asp Thr Leu Ser Leu

1085 1090 1095

Ile Gln Gly Gly Met Thr Ala Gly Ser Thr Thr Ser Glu Thr Ser

1100 1105 1110

Cys Glu Thr Val Arg Gln Ser Cys Val Ala Leu Val Glu Lys Leu

1115 1120 1125

Asn Pro Ile Lys Glu Ser Leu Glu Ala Lys Ser Asn Thr Val Glu

1130 1135 1140

Trp Ser Ala Leu Ile Ala Gln Ala Ser Met Ala Ser Val Asn Leu

1145 1150 1155

Ser Ala Gln Pro Tyr Trp Thr Pro Asp Pro Ser Phe Lys Ser Tyr

1160 1165 1170

Leu Asn Tyr Gly Ala Gly Thr Ser Glu Val Glu Val Asp Ile Leu

1175 1180 1185

Thr Gly Ala Thr Thr Ile Leu Arg Ser Asp Leu Val Tyr Asp Cys

1190 1195 1200

Gly Gln Ser Leu Asn Pro Ala Val Asp Leu Gly Gln Ile Glu Gly

1205 1210 1215

Cys Phe Val Gln Gly Ile Gly Phe Phe Thr Asn Glu Asp Tyr Lys

1220 1225 1230

Thr Asn Ser Asp Gly Leu Val Ile His Asp Gly Thr Trp Thr Tyr

1235 1240 1245

Lys Ile Pro Thr Val Asp Asn Ile Pro Lys Glu Phe Asn Val Glu

1250 1255 1260

Met Phe Asn Ser Ala Pro Asp Lys Lys Arg Val Leu Ser Ser Lys

1265 1270 1275

Ala Ser Gly Glu Pro Pro Leu Val Leu Ala Thr Ser Val His Cys

1280 1285 1290

Ala Met Arg Glu Ala Ile Arg Ala Ala Arg Lys Glu Phe Ser Val

1295 1300 1305

Ser Thr Ser Pro Ala Lys Ser Ala Val Thr Phe Gln Met Asp Val

1310 1315 1320

Pro Ala Thr Met Pro Val Val Lys Glu Leu Cys Gly Leu Asp Val

1325 1330 1335

Val Glu Arg Tyr Leu Glu Asn Val Ser Ala Ala Ser Ala Gly Pro

1340 1345 1350

Asn Thr Ala Lys Ala

1355

<210> 156

<211> 747

<212> DNA

<213> 新月柄杆菌(Caulobacter crescentus)

<400> 156

atgtcctcag ccatctatcc cagcctgaag ggcaagcgcg tcgtcatcac cggcggcggc 60

tcgggcatcg gggccggcct caccgccggc ttcgcccgtc agggcgcgga ggtgatcttc 120

ctcgacatcg ccgacgagga ctccagggct cttgaggccg agctggccgg ctcgccgatc 180

ccgccggtct acaagcgctg cgacctgatg aacctcgagg cgatcaaggc ggtcttcgcc 240

gagatcggcg acgtcgacgt gctggtcaac aacgccggca atgacgaccg ccacaagctg 300

gccgacgtga ccggcgccta ttgggacgag cggatcaacg tcaacctgcg ccacatgctg 360

ttctgcaccc aggccgtcgc gccgggcatg aagaagcgtg gcggcggggc ggtgatcaac 420

ttcggttcga tcagctggca cctggggctt gaggacctcg tcctctacga aaccgccaag 480

gccggcatcg aaggcatgac ccgcgcgctg gcccgggagc tgggtcccga cgacatccgc 540

gtcacctgcg tggtgccggg caacgtcaag accaagcgcc aggagaagtg gtacacgccc 600

gaaggcgagg cccagatcgt ggcggcccaa tgcctgaagg gccgcatcgt cccggagaac 660

gtcgccgcgc tggtgctgtt cctggcctcg gatgacgcgt cgctctgcac cggccacgaa 720

tactggatcg acgccggctg gcgttga 747

<210> 157

<211> 870

<212> DNA

<213> 新月柄杆菌(Caulobacter crescentus)

<400> 157

atgaccgctc aagtcacttg cgtatgggat ctgaaggcca cgttgggcga aggcccgatc 60

tggcatggcg acaccctgtg gttcgtcgac atcaagcagc gtaaaatcca caactaccac 120

cccgccaccg gcgagcgctt cagcttcgac gcgccggatc aggtgacctt cctcgcgccg 180

atcgtcggcg cgaccggctt tgtcgtcggt ctgaagaccg ggattcaccg cttccacccg 240

gccacgggct tcagcctgct gctcgaggtc gaggacgcgg cgctgaacaa ccgccccaac 300

gacgccacgg tcgacgcgca aggccgtctg tggttcggca ccatgcacga cggggaagag 360

aacaatagcg gctcgctcta tcggatggac ctcaccggcg tcgcccggat ggaccgcgac 420

atctgcatca ccaacggccc gtgcgtctcg cccgacggca agaccttcta ccacaccgac 480

accctggaaa agacgatcta cgccttcgac ctggccgagg acggcctgct gtcgaacaag 540

cgcgtcttcg tgcagttcgc cctgggcgac gatgtctatc cggacggttc ggtcgtcgat 600

tccgaaggct atctgtggac cgccctgtgg ggcggtttcg gcgcggtccg cttctcgccg 660

caaggcgacg ccgtgacgcg catcgaactg cccgccccca acgtcaccaa gccctgcttc 720

ggcgggcctg acctgaagac cctctatttc accaccgccc gcaagggcct gagcgacgag 780

accctggccc agtacccgct ggccggcggt gtgttcgccg ttccggtcga tgtggccggc 840

caaccccagc atgaggtccg ccttgtctaa 870

<210> 158

<211> 1968

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 158

atgtctgttc gcaatatttt tgctgacgag agccacgata tttacaccgt cagaacgcac 60

gccgatggcc cggacggcga actcccatta accgcagaga tgcttatcaa ccgcccgagc 120

ggggatctgt tcggtatgac catgaatgcc ggaatgggtt ggtctccgga cgagctggat 180

cgggacggta ttttactgct cagtacactc ggtggcttac gcggcgcaga cggtaaaccc 240

gtggcgctgg cgttgcacca ggggcattac gaactggaca tccagatgaa agcggcggcc 300

gaggttatta aagccaacca tgccctgccc tatgccgtgt acgtctccga tccttgtgac 360

gggcgtactc agggtacaac ggggatgttt gattcgctac cataccgaaa tgacgcatcg 420

atggtaatgc gccgccttat tcgctctctg cccgacgcga aagcagttat tggtgtggcg 480

agttgcgata aggggcttcc ggccaccatg atggcactcg ccgcgcagca caacatcgca 540

accgtgctgg tccccggcgg cgcgacgctg cccgcaaagg atggagaaga caacggcaag 600

gtgcaaacca ttggcgcacg cttcgccaat ggcgaattat ctctacagga cgcacgccgt 660

gcgggctgta aagcctgtgc ctcttccggc ggcggctgtc aatttttggg cactgccggg 720

acatctcagg tggtggccga aggattggga ctggcaatcc cacattcagc cctggcccct 780

tccggtgagc ctgtgtggcg ggagatcgcc agagcttccg cgcgagctgc gctgaacctg 840

agtcaaaaag gcatcaccac ccgggaaatt ctcaccgata aagcgataga gaatgcgatg 900

acggtccatg ccgcgttcgg tggttcaaca aacctgctgt tacacatccc ggcaattgct 960

caccaggcag gttgccatat cccgaccgtt gatgactgga tccgcatcaa caagcgcgtg 1020

ccccgactgg tgagcgtact gcctaatggc ccggtttatc atccaacggt caatgccttt 1080

atggcaggtg gtgtgccgga agtcatgttg catctgcgca gcctcggatt gttgcatgaa 1140

gacgttatga cggttaccgg cagcacgctg aaagaaaacc tcgactggtg ggagcactcc 1200

gaacggcgtc agcggttcaa gcaactcctg ctcgatcagg aacaaatcaa cgctgacgaa 1260

gtgatcatgt ctccgcagca agcaaaagcg cgcggattaa cctcaactat caccttcccg 1320

gtgggcaata ttgcgccaga aggttcggtg atcaaatcca ccgccattga cccctcgatg 1380

attgatgagc aaggtatcta ttaccataaa ggtgtggcga aggtttatct gtccgagaaa 1440

agtgcgattt acgatatcaa acatgacaag atcaaggcgg gcgatattct ggtcattatt 1500

ggcgttggac cttcaggtac agggatggaa gaaacctacc aggttaccag tgccctgaag 1560

catctgtcat acggtaagca tgtttcgtta atcaccgatg cacgtttctc gggcgtttct 1620

actggcgcgt gcatcggcca tgtggggcca gaagcgctgg ccggaggccc catcggtaaa 1680

ttacgcaccg gggatttaat tgaaattaaa attgattgtc gcgagcttca cggcgaagtc 1740

aatttcctcg gaacccgtag cgatgaacaa ttaccttcac aggaggaggc aactgcaata 1800

ttaaatgcca gacccagcca tcaggattta cttcccgatc ctgaattgcc agatgatacc 1860

cggctatggg caatgcttca ggccgtgagt ggtgggacat ggaccggttg tatttatgat 1920

gtaaacaaaa ttggcgcggc tttgcgcgat tttatgaata aaaactga 1968

<210> 159

<211> 1383

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 159

atgacgcaat taaccatgaa agacaaaatt ggctacgggc tgggagacac cgcctgcggc 60

ttcgtctggc aggccacgat gttcctgctg gcctatttct acaccgacgt cttcggcctg 120

tcggcgggga ttatgggcac gctgtttttg gtctcccgcg tgctcgacgc cgtcaccgac 180

ccgctgatgg ggctgctggt agaccgcacc cgcacgcggc acggccagtt ccgcccgttc 240

ctgctgtggg gggccatccc gttcggcatc gtctgcgtgc tgaccttcta cacgccggac 300

ttctccgcac agggcaagat catctacgcc tgcgtgacct acattctcct gaccctggtc 360

tacaccttcg ttaacgtgcc gtactgcgcc atgccgggcg tcatcaccgc cgacccgaaa 420

gagcgtcacg ccctgcagtc ctggcgcttc ttcctggcgg cggcgggctc gctcgctatc 480

agcggcatcg cgctgccgct ggtgagcatc atcggcaaag gggacgagca ggtgggctac 540

ttcggcgcca tgtgcgtgct ggggctgagc ggcgtggtgc tgctctacgt ctgcttcttc 600

acgaccaaag agcgctacac ctttgaggtg cagccgggct cgtcggtggc gaaagacctt 660

aagctgctgc tgggcaacag ccagtggcgc atcatgtgcg cgttcaagat gatggcgacc 720

tgctccaacg tggtgcgcgg cggggcgacg ctctacttcg tgaaatacgt gatggatcac 780

ccggagttgg cgacccagtt tttactttac ggcagcctcg ccaccatgtt cggctcgctt 840

tgctcctcac gcctgctggg ccgcttcgac cgcgtcaccg ccttcaagtg gatcatcgtc 900

gcctactcgc tgatcagcct gctgattttc gtcaccccgg cggagcacat cgcgctcatt 960

tttgccctca acatcctgtt cctgttcgtc tttaatacca ccacgccgct gcagtggctg 1020

atggcttctg acgtggtgga ctacgaggag agccgcagcg gtcgccgcct cgacgggctg 1080

gtgttctcca cctacctgtt cagcctgaag attggcctgg cgattggcgg ggcggtggtg 1140

ggctggatcc tggcgtacgt caactattcc gccagcagca gcgtgcagcc ggttgaggtg 1200

ctcaccacca tcaaaattct gttctgcgtg gtgccggtgg tgctctacgc gggcatgttc 1260

atcatgctgt cgctctacaa gctcaccgat gcccgcgtgg aggccatcag ccggcagctg 1320

attaagcacc gcgcggcgca gggcgaggcc gttcccgacg ccgcgacagc cgcatcccat 1380

taa 1383

<210> 160

<211> 1041

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 160

atgcagaaca tcatccgaaa aggaggaact atgaaggctg cagttgttac gaaggatcat 60

catgttgacg ttacgtataa aacactgcgc tcactgaaac atggcgaagc cctgctgaaa 120

atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga ttttggtgac 180

aaaaccggcg taattctggg ccatgaaggc atcggtgtgg tggcagaagt gggtccaggt 240

gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt ggttctacga aggatgcggt 300

cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa aaatgccgga 360

tacagcgttg atggcgggat ggcggaagag tgcatcgtgg tcgccgatta cgcggtaaaa 420

gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg agtcaccacc 480

tacaaagccg ttaagctgtc aaaaattcgt ccagggcagt ggattgctat ctacggtctt 540

ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg tctttaacgc caaagtgatc 600

gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggcgc agatttagcg 660

attaactcac acaccgaaga cgccgccaaa attgtgcagg agaaaactgg tggcgctcac 720

gctgcggtgg taacagcggt agctaaagct gcgtttaact cggcagttga tgctgtccgt 780

gcaggcggtc gtgttgtggc tgtcggtcta ccgccggagt ctatgagcct ggatatccca 840

cgtcttgtgc tggatggtat tgaagtggtc ggttcgctgg tcggcacgcg ccaggattta 900

actgaagcct tccagtttgc cgccgaaggt aaagtggtgc cgaaagtcgc cctgcgtccg 960

ttagcggaca tcaacaccat ctttactgag atggaagaag gcaaaatccg tggccgcatg 1020

gtgattgatt tccgtcacta a 1041

<210> 161

<211> 1164

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 161

atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct 60

ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg cggcggcagc 120

gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg 180

gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg 240

gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc 300

accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg 360

caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca 420

gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag 480

caggcgttcc attctgccca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc 540

tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg 600

gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt 660

ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg 720

cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cgctggcgta 780

ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat 840

cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgataccaag 900

cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgatgat 960

gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg 1020

acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg 1080

gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc 1140

cgtatatacg aagccgcccg ctaa 1164

<210> 162

<211> 1587

<212> DNA

<213> 恶臭假单胞菌(Pseudomonas putida)

<400> 162

atggcttcgg tacacggcac cacatacgaa ctcttgcgac gtcaaggcat cgatacggtc 60

ttcggcaatc ctggctcgaa cgagctcccg tttttgaagg actttccaga ggactttcga 120

tacatcctgg ctttgcagga agcgtgtgtg gtgggcattg cagacggcta tgcgcaagcc 180

agtcggaagc cggctttcat taacctgcat tctgctgctg gtaccggcaa tgctatgggt 240

gcactcagta acgcctggaa ctcacattcc ccgctgatcg tcactgccgg ccagcagacc 300

agggcgatga ttggcgttga agctctgctg accaacgtcg atgccgccaa cctgccacga 360

ccacttgtca aatggagcta cgagcccgca agcgcagcag aagtccctca tgcgatgagc 420

agggctatcc atatggcaag catggcgcca caaggccctg tctatctttc ggtgccatat 480

gacgattggg ataaggatgc tgatcctcag tcccaccacc tttttgatcg ccatgtcagt 540

tcatcagtac gcctgaacga ccaggatctc gatattctgg tgaaagctct caacagcgca 600

tccaacccgg cgatcgtcct gggcccggac gtcgacgcag caaatgcgaa cgcagactgc 660

gtcatgttgg ccgaacgcct caaagctccg gtttgggttg cgccatccgc tccacgctgc 720

ccattcccta cccgtcatcc ttgcttccgt ggattgatgc cagctggcat cgcagcgatt 780

tctcagctgc tcgaaggtca cgatgtggtt ttggtaatcg gcgctccagt gttccgttac 840

caccaatacg acccaggtca atatctcaaa cctggcacgc gattgatttc ggtgacctgc 900

gacccgctcg aagctgcacg cgcgccaatg ggcgatgcga tcgtggcaga cattggtgcg 960

atggctagcg ctcttgccaa cttggttgaa gagagcagcc gccagctccc aactgcagct 1020

ccggaacccg cgaaggttga ccaagacgct ggccgacttc acccagagac agtgttcgac 1080

acactgaacg acatggcccc ggagaatgcg atttacctga acgagtcgac ttcaacgacc 1140

gcccaaatgt ggcagcgcct gaacatgcgc aaccctggta gctactactt ctgtgcagct 1200

ggcggactgg gcttcgccct gcctgcagca attggcgttc aactcgcaga acccgagcga 1260

caagtcatcg ccgtcattgg cgacggatcg gcgaactaca gcattagtgc gttgtggact 1320

gcagctcagt acaacatccc cactatcttc gtgatcatga acaacggcac ctacggtgcg 1380

ttgcgatggt ttgccggcgt tctcgaagca gaaaacgttc ctgggctgga tgtgccaggg 1440

atcgacttcc gcgcactcgc caagggctat ggtgtccaag cgctgaaagc cgacaacctt 1500

gagcagctca agggttcgct acaagaagcg ctttctgcca aaggcccggt acttatcgaa 1560

gtaagcaccg taagcccggt gaagtga 1587

<210> 163

<211> 1323

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 163

atgcaagcct attttgacca gctcgatcgc gttcgttatg aaggctcaaa atcctcaaac 60

ccgttagcat tccgtcacta caatcccgac gaactggtgt tgggtaagcg tatggaagag 120

cacttgcgtt ttgccgcctg ctactggcac accttctgct ggaacggggc ggatatgttt 180

ggtgtggggg cgtttaatcg tccgtggcag cagcctggtg aggcactggc gttggcgaag 240

cgtaaagcag atgtcgcatt tgagtttttc cacaagttac atgtgccatt ttattgcttc 300

cacgatgtgg atgtttcccc tgagggcgcg tcgttaaaag agtacatcaa taattttgcg 360

caaatggttg atgtcctggc aggcaagcaa gaagagagcg gcgtgaagct gctgtgggga 420

acggccaact gctttacaaa ccctcgctac ggcgcgggtg cggcgacgaa cccagatcct 480

gaagtcttca gctgggcggc aacgcaagtt gttacagcga tggaagcaac ccataaattg 540

ggcggtgaaa actatgtcct gtggggcggt cgtgaaggtt acgaaacgct gttaaatacc 600

gacttgcgtc aggagcgtga acaactgggc cgctttatgc agatggtggt tgagcataaa 660

cataaaatcg gtttccaggg cacgttgctt atcgaaccga aaccgcaaga accgaccaaa 720

catcaatatg attacgatgc cgcgacggtc tatggcttcc tgaaacagtt tggtctggaa 780

aaagagatta aactgaacat tgaagctaac cacgcgacgc tggcaggtca ctctttccat 840

catgaaatag ccaccgccat tgcgcttggc ctgttcggtt ctgtcgacgc caaccgtggc 900

gatgcgcaac tgggctggga caccgaccag ttcccgaaca gtgtggaaga gaatgcgctg 960

gtgatgtatg aaattctcaa agcaggcggt ttcaccaccg gtggtctgaa cttcgatgcc 1020

aaagtacgtc gtcaaagtac tgataaatat gatctgtttt acggtcatat cggcgcgatg 1080

gatacgatgg cactggcgct gaaaattgca gcgcgcatga ttgaagatgg cgagctggat 1140

aaacgcatcg cgcagcgtta ttccggctgg aatagcgaat tgggccagca aatcctgaaa 1200

ggccaaatgt cactggcaga tttagccaaa tatgctcagg aacatcattt gtctccggtg 1260

catcagagtg gtcgccagga acaactggaa aatctggtaa accattatct gttcgacaaa 1320

taa 1323

<210> 164

<211> 1455

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 164

atgtatatcg ggatagatct tggcacctcg ggcgtaaaag ttattttgct caacgagcag 60

ggtgaggtgg ttgctgcgca aacggaaaag ctgaccgttt cgcgcccgca tccactctgg 120

tcggaacaag acccggaaca gtggtggcag gcaactgatc gcgcaatgaa agctctgggc 180

gatcagcatt ctctgcagga cgttaaagca ttgggtattg ccggccagat gcacggagca 240

accttgctgg atgctcagca acgggtgtta cgccctgcca ttttgtggaa cgacgggcgc 300

tgtgcgcaag agtgcacttt gctggaagcg cgagttccgc aatcgcgggt gattaccggc 360

aacctgatga tgcccggatt tactgcgcct aaattgctat gggttcagcg gcatgagccg 420

gagatattcc gtcaaatcga caaagtatta ttaccgaaag attacttgcg tctgcgtatg 480

acgggggagt ttgccagcga tatgtctgac gcagctggca ccatgtggct ggatgtcgca 540

aagcgtgact ggagtgacgt catgctgcag gcttgcgact tatctcgtga ccagatgccc 600

gcattatacg aaggcagcga aattactggt gctttgttac ctgaagttgc gaaagcgtgg 660

ggtatggcga cggtgccagt tgtcgcaggc ggtggcgaca atgcagctgg tgcagttggt 720

gtgggaatgg ttgatgctaa tcaggcaatg ttatcgctgg ggacgtcggg ggtctatttt 780

gctgtcagcg aagggttctt aagcaagcca gaaagcgccg tacatagctt ttgccatgcg 840

ctaccgcaac gttggcattt aatgtctgtg atgctgagtg cagcgtcgtg tctggattgg 900

gccgcgaaat taaccggcct gagcaatgtc ccagctttaa tcgctgcagc tcaacaggct 960

gatgaaagtg ccgagccagt ttggtttctg ccttatcttt ccggcgagcg tacgccacac 1020

aataatcccc aggcgaaggg ggttttcttt ggtttgactc atcaacatgg ccccaatgaa 1080

ctggcgcgag cagtgctgga aggcgtgggt tatgcgctgg cagatggcat ggatgtcgtg 1140

catgcctgcg gtattaaacc gcaaagtgtt acgttgattg ggggcggggc gcgtagtgag 1200

tactggcgtc agatgctggc ggatatcagc ggtcagcagc tcgattaccg tacggggggg 1260

gatgtggggc cagcactggg cgcagcaagg ctggcgcaga tcgcggcgaa tccagagaaa 1320

tcgctcattg aattgttgcc gcaactaccg ttagaacagt cgcatctacc agatgcgcag 1380

cgttatgccg cttatcagcc acgacgagaa acgttccgtc gcctctatca gcaacttctg 1440

ccattaatgg cgtaa 1455

<210> 165

<211> 906

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 165

atgaaaaaat tcagcggcat tattccaccg gtatccagca cgtttcatcg tgacggaacc 60

cttgataaaa aggcaatgcg cgaagttgcc gacttcctga ttaataaagg ggtcgacggg 120

ctgttttatc tgggtaccgg tggtgaattt agccaaatga atacagccca gcgcatggca 180

ctcgccgaag aagctgtaac cattgtcgac gggcgagtgc cggtattgat tggcgtcggt 240

tccccttcca ctgacgaagc ggtcaaactg gcgcagcatg cgcaagccta cggcgctgat 300

ggtatcgtcg ccatcaaccc ctactactgg aaagtcgcac cacgaaatct tgacgactat 360

taccagcaga tcgcccgtag cgtcacccta ccggtgatcc tgtacaactt tccggatctg 420

acgggtcagg acttaacccc ggaaaccgtg acgcgtctgg ctctgcaaaa cgagaatatc 480

gttggcatca aagacaccat cgacagcgtt ggtcacttgc gtacgatgat caacacagtt 540

aagtcggtac gcccgtcgtt ttcggtattc tgcggttacg atgatcattt gctgaatacg 600

atgctgctgg gcggcgacgg tgcgataacc gccagcgcta actttgctcc ggaactctcc 660

gtcggcatct accgcgcctg gcgtgaaggc gatctggcga ccgctgcgac gctgaataaa 720

aaactactac aactgcccgc tatttacgcc ctcgaaacac cgtttgtctc actgatcaaa 780

tacagcatgc agtgtgtcgg gctgcctgta gagacatatt gcttaccacc gattcttgaa 840

gcatctgaag aagcaaaaga taaagtccac gtgctgctta ccgcgcaggg cattttacca 900

gtctga 906

<210> 166

<211> 909

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 166

atgccgcagt ccgcgttgtt cacgggaatc attccccctg tctccaccat ttttaccgcc 60

gacggccagc tcgataagcc gggcaccgcc gcgctgatcg acgatctgat caaagcaggc 120

gttgacggcc tgttcttcct gggcagcggt ggcgagttct cccagctcgg cgccgaagag 180

cgtaaagcca ttgcccgctt tgctatcgat catgtcgatc gtcgcgtgcc ggtgctgatc 240

ggcaccggcg gcaccaacgc ccgggaaacc atcgaactca gccagcacgc gcagcaggcg 300

ggcgcggacg gcatcgtggt gatcaacccc tactactgga aagtgtcgga agcgaacctg 360

atccgctatt tcgagcaggt ggccgacagc gtcacgctgc cggtgatgct ctataacttc 420

ccggcgctga ccgggcagga tctgactccg gcgctggtga aaaccctcgc cgactcgcgc 480

agcaatatta tcggcatcaa agacaccatc gactccgtcg cccacctgcg cagcatgatc 540

cataccgtca aaggtgccca tccgcacttc accgtgctct gcggctacga cgatcatctg 600

ttcaataccc tgctgctcgg cggcgacggg gcgatatcgg cgagcggcaa ctttgccccg 660

caggtgtcgg tgaatcttct gaaagcctgg cgcgacgggg acgtggcgaa agcggccggg 720

tatcatcaga ccttgctgca aattccgcag atgtatcagc tggatacgcc gtttgtgaac 780

gtgattaaag aggcgatcgt gctctgcggt cgtcctgtct ccacgcacgt gctgccgccc 840

gcctcgccgc tggacgagcc gcgcaaggcg cagctgaaaa ccctgctgca acagctcaag 900

ctttgctga 909

<210> 167

<211> 975

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 167

atgaagccgt ccgttatcct ctacaaagcc ttacctgatg atttactgca acgcctgcaa 60

gagcatttca ccgttcacca ggtggcaaac ctcagcccac aaaccgtcga acaaaatgca 120

gcaatttttg ccgaagctga aggtttactg ggttcaaacg agaatgtaaa tgccgcattg 180

ctggaaaaaa tgccgaaact gcgtgccaca tcaacgatct ccgtcggcta tgacaatttt 240

gatgtcgatg cgcttaccgc ccgaaaaatt ctgctgatgc acacgccaac cgtattaaca 300

gaaaccgtcg ccgatacgct gatggcgctg gtgttgtcta ccgctcgtcg ggttgtggag 360

gtagcagaac gggtaaaagc aggcgaatgg accgcgagca taggcccgga ctggtacggc 420

actgacgttc accataaaac actgggcatt gtcgggatgg gacggatcgg catggcgctg 480

gcacaacgtg cgcactttgg cttcaacatg cccatcctct ataacgcgcg ccgccaccat 540

aaagaagcag aagaacgctt caacgcccgc tactgcgatt tggatactct gttacaagag 600

tcagatttcg tttgcctgat cctgccgtta actgatgaga cgcatcatct gtttggcgca 660

gaacaattcg ccaaaatgaa atcctccgcc attttcatta atgccggacg tggcccggtg 720

gttgacgaaa atgcactgat cgcagcattg cagaaaggcg aaattcacgc tgccgggctg 780

gatgtcttcg aacaagagcc actgtccgta gattcgccgt tgctctcaat ggccaacgtc 840

gtcgcagtac cgcatattgg atctgccacc catgagacgc gttatggcat ggccgcctgt 900

gccgtggata atttgattga tgcgttacaa ggaaaggttg agaagaactg tgtgaatccg 960

cacgtcgcgg actaa 975

<210> 168

<211> 939

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 168

atggatatca tcttttatca cccaacgttc gatacccaat ggtggattga ggcactgcgc 60

aaagctattc ctcaggcaag agtcagagca tggaaaagcg gagataatga ctctgctgat 120

tatgctttag tctggcatcc tcctgttgaa atgctggcag ggcgcgatct taaagcggtg 180

ttcgcactcg gggccggtgt tgattctatt ttgagcaagc tacaggcaca ccctgaaatg 240

ctgaaccctt ctgttccact ttttcgcctg gaagataccg gtatgggcga gcaaatgcag 300

gaatatgctg tcagtcaggt gctgcattgg tttcgacgtt ttgacgatta tcgcatccag 360

caaaatagtt cgcattggca accgctgcct gaatatcatc gggaagattt taccatcggc 420

attttgggcg caggcgtact gggcagtaaa gttgctcaga gtctgcaaac ctggcgcttt 480

ccgctgcgtt gctggagtcg aacccgtaaa tcgtggcctg gcgtgcaaag ctttgccgga 540

cgggaagaac tgtctgcatt tctgagccaa tgtcgggtat tgattaattt gttaccgaat 600

acccctgaaa ccgtcggcat tattaatcaa caattactcg aaaaattacc ggatggcgcg 660

tatctcctca acctggcgcg tggtgttcat gttgtggaag atgacctgct cgcggcgctg 720

gatagcggca aagttaaagg cgcaatgttg gatgttttta atcgtgaacc cttaccgcct 780

gaaagtccgc tctggcaaca tccacgcgtg acgataacac cacatgtcgc cgcgattacc 840

cgtcccgctg aagctgtgga gtacatttct cgcaccattg cccagctcga aaaaggggag 900

agggtctgcg ggcaagtcga ccgcgcacgc ggctactaa 939

<210> 169

<211> 1434

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 169

atgtttaaga atgcatttgc taacctgcaa aaggtcggta aatcgctgat gctgccggta 60

tccgtactgc ctatcgcagg tattctgctg ggcgtcggtt ccgcgaattt cagctggctg 120

cccgccgttg tatcgcatgt tatggcagaa gcaggcggtt ccgtctttgc aaacatgcca 180

ctgatttttg cgatcggtgt cgccctcggc tttaccaata acgatggcgt atccgcgctg 240

gccgcagttg ttgcctatgg catcatggtt aaaaccatgg ccgtggttgc gccactggta 300

ctgcatttac ctgctgaaga aatcgcctct aaacacctgg cggatactgg cgtactcgga 360

gggattatct ccggtgcgat cgcagcgtac atgtttaacc gtttctaccg tattaagctg 420

cctgagtatc ttggcttctt tgccggtaaa cgctttgtgc cgatcatttc tggcctggct 480

gccatcttta ctggcgttgt gctgtccttc atttggccgc cgattggttc tgcaatccag 540

accttctctc agtgggctgc ttaccagaac ccggtagttg cgtttggcat ttacggtttc 600

atcgaacgtt gcctggtacc gtttggtctg caccacatct ggaacgtacc tttccagatg 660

cagattggtg aatacaccaa cgcagcaggt caggttttcc acggcgacat tccgcgttat 720

atggcgggtg acccgactgc gggtaaactg tctggtggct tcctgttcaa aatgtacggt 780

ctgccagctg ccgcaattgc tatctggcac tctgctaaac cagaaaaccg cgcgaaagtg 840

ggcggtatta tgatctccgc ggcgctgacc tcgttcctga ccggtatcac cgagccgatc 900

gagttctcct tcatgttcgt tgcgccgatc ctgtacatca tccacgcgat tctggcaggc 960

ctggcattcc caatctgtat tcttctgggg atgcgtgacg gtacgtcgtt ctcgcacggt 1020

ctgatcgact tcatcgttct gtctggtaac agcagcaaac tgtggctgtt cccgatcgtc 1080

ggtatcggtt atgcgattgt ttactacacc atcttccgcg tgctgattaa agcactggat 1140

ctgaaaacgc cgggtcgtga agacgcgact gaagatgcaa aagcgacagg taccagcgaa 1200

atggcaccgg ctctggttgc tgcatttggt ggtaaagaaa acattactaa cctcgacgca 1260

tgtattaccc gtctgcgcgt cagcgttgct gatgtgtcta aagtggatca ggccggcctg 1320

aagaaactgg gcgcagcggg cgtagtggtt gctggttctg gtgttcaggc gattttcggt 1380

actaaatccg ataacctgaa aaccgagatg gatgagtaca tccgtaacca ctaa 1434

<210> 170

<211> 510

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 170

atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga taccggaact 60

attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc 120

gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg taacaaaatg 180

gtcgcgccag tagacggcac cattggtaaa atctttgaaa ccaaccacgc attctctatc 240

gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa 300

ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc 360

attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt 420

atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt 480

gaaaccccgg ttatccgcat caagaagtaa 510

<210> 171

<211> 258

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 171

atgttccagc aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc 60

cagtttgtaa aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa 120

agcgccagcg cgaaaagcct gtttaaactg cagactctgg gcctgactca aggtaccgtt 180

gtgactatct ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg 240

atggcggaac tcgagtaa 258

<210> 172

<211> 1254

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 172

atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt 60

tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc 120

agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa 180

ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt 240

accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa 300

tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc 360

ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt 420

ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc 480

atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc 540

gccgttttac tctttttcgc caaaacggat gcgccctctt ctgccacggt tgccaatgcg 600

gtaggtgcca accattcggc atttagcctt aagctggcac tggaactgtt cagacagcca 660

aaactgtggt ttttgtcact gtatgttatt ggcgtttcct gcacctacga tgtttttgac 720

caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg tacgcgggta 780

tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt ctttgcgcca 840

ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac tattatgtct 900

gtacgtatta ttggctcatc gttcgccacc tcagcgctgg aagtggttat tctgaaaacg 960

ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct ttaaatatat taccagccag 1020

tttgaagtgc gtttttcagc gacgatttat ctggtctgtt tctgcttctt taagcaactg 1080

gcgatgattt ttatgtctgt actggcgggc aatatgtatg aaagcatcgg tttccagggc 1140

gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt gttcacgctt 1200

agcggccccg gcccgctttc cctgctgcgt cgtcaggtga atgaagtcgc ttaa 1254

<210> 173

<211> 1545

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 173

atggatgtca ttaaaaagaa acattggtgg caaagcgacg cgctgaaatg gtcagtgcta 60

ggtctgctcg gcctgctggt gggttacctt gttgttttaa tgtacgcaca aggggaatac 120

ctgttcgcca ttaccacgct gatattgagt tcagcggggc tgtatatttt cgccaatcgt 180

aaagcctacg cctggcgcta tgtttacccg ggaatggctg gaatgggatt attcgtcctc 240

ttccctctgg tctgcaccat cgccattgcc ttcaccaact acagcagcac taaccagctg 300

acttttgaac gtgcgcagga agtgttgtta gatcgctcct ggcaagcagg caaaacctat 360

aactttggtc tttacccggc gggcgatgag tggcaactgg cgctcagcga cggcgaaacc 420

ggcaaaaatt acctctccga cgcttttaaa tttggcggcg agcaaaaact gcaactgaaa 480

gaaacgaccg cccagcccga aggcgaacgc gcgaatctgc gcgtgattac ccagaatcgt 540

caggcgctga gtgacattac cgccattctg ccggatggca acaaagtgat gatgagctcc 600

ctgcgccagt tttctggcac gcagccgctc tacacactcg acggtgacgg cacgttgacg 660

aataatcaga gcggcgtgaa atatcgtccg aataaccaaa ttggctttta ccagtccatt 720

accgccgacg gcaactgggg tgatgaaaag ctaagccccg gttacaccgt gaccaccggc 780

tggaaaaact ttacccgcgt ctttaccgac gaaggcattc agaaaccgtt cctcgccatt 840

ttcgtctgga ccgtggtgtt ctcgctgatc actgtctttt taacggtggc ggtcggcatg 900

gttctggcgt gtctggtgca gtgggaagcg ttgcgcggca aagcggtcta tcgcgtcctg 960

ctgattctgc cctacgcggt gccatcgttc atttcaatct tgattttcaa agggttgttt 1020

aaccagagct tcggtgaaat caacatgatg ttgagcgcgc tgtttggcgt gaagcccgcc 1080

tggttcagcg atccgaccac cgcccgcacg atgctaatta tcgtcaatac ctggctgggt 1140

tatccgtaca tgatgatcct ctgcatgggc ttgctgaaag cgattccgga cgatttgtat 1200

gaagcctcag caatggatgg cgcaggtccg ttccagaact tctttaagat tacgctgccg 1260

ctgctgatta aaccgctgac gccgctgatg atcgccagct tcgcctttaa ctttaacaac 1320

ttcgtgctga ttcaactgtt aaccaacggc ggcccggatc gtcttggcac gaccacgcca 1380

gccggttata ccgacctgct tgttaactac acctaccgca tcgcttttga aggcggcggg 1440

ggtcaggact tcggtctggc ggcagcaatt gccacgctga tcttcctgct ggtgggtgcg 1500

ctggcgatag tgaacctgaa agccacgcga atgaagtttg attaa 1545

<210> 174

<211> 891

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 174

atggcaatgg tccaaccgaa atcgcaaaaa gctcgtttat ttattactca cctgctactg 60

ctacttttta tcgcagcgat tatgttcccg ctgctgatgg tcgtcgctat ctcgctgcgt 120

cagggaaact ttgcgaccgg cagcctgatc ccggagcaaa tctcctggga tcactggaaa 180

ctggcgttag gttttagcgt tgaacaggct gatggtcgca ttacgccacc gccattcccg 240

gtactgctgt ggctgtggaa ctcggtaaag gtcgccggga tttccgcgat tggcattgtg 300

gcgctctcca ccacctgcgc ctacgctttc gcccgtatgc gctttccagg caaagcgacg 360

ctgctgaaag gaatgctgat tttccagatg ttcccggcag tactttcact ggtcgcgttg 420

tatgcgttgt ttgatcgtct gggtgagtac attccattca ttggcctgaa tactcacggc 480

ggcgtaattt tcgcgtatct gggtgggatt gcgctgcatg tctggaccat caaaggctat 540

ttcgaaacca tcgacagttc gctggaagaa gctgctgcgc tggatggtgc gacaccgtgg 600

caggccttcc gccttgtcct gttgccgctg tcagtaccga ttctggcggt ggtattcatc 660

ctgtcgttta tcgctgccat tactgaagtt ccggtcgcgt cgctgttact gcgtgacgta 720

aacagctaca ccctggccgt ggggatgcag caatacctca acccgcaaaa ctacctgtgg 780

ggtgactttg ccgccgctgc cgtgatgtct gcattaccga tcaccatcgt cttcttgctg 840

gctcaacgct ggctggtcaa cggcctgacg gcaggtggtg tgaaaggtta a 891

<210> 175

<211> 6546

<212> DNA

<213> 鼠伤寒沙门氏菌(Salmonella typhimurium)

<400> 175

atgaatgcaa aagtttgggt tctgggcgac gcggtggtgg acctgctgcc ggagagcgaa 60

gggcgcctgc tgcagtgccc tggaggcgcg ccggctaacg tggcggtagg ggttgcccgc 120

cttggcggca acagcggatt tatcggcgcc gtcggcggtg acccgtttgg ccgctacatg 180

cgtcataccc tgcaacagga gcaggtcgac gtcagccata tgtatctcga cgatcagcac 240

cgcacgtcca ctgtggtcgt cgaccttgac gaccaggggg aacgcacctt tacctttatg 300

gtacgcccca gcgcggacct gttcctggtt gaagaagacc tgccacagtt tgccgccgga 360

cagtggttgc acgtctgctc catcgcgctc agcgccgagc ccagccgtag cactaccttc 420

gcggcgatgg agagcatcag gtctgccggc ggtcgggtca gctttgaccc taatattcgt 480

cccgatctct ggcaggatca ggctttgctg ctagcctgcc tcgatcgcgc tttgcacatg 540

gccaacgtgg taaagctatc ggaagaggag ctggtcttca tcagcagcag taatgattta 600

gcatacggaa tcgccagcgt aacggagcgc tatcagccag aattgctact ggtgacccgg 660

ggcaaagcgg gggtgcttgc cgcgtttcag cagaagttta cccatttcaa cgcccggcct 720

gtggccagcg tggacaccac cggcgcggga gacgcatttg tcgccggact gctcgccagc 780

cttgcggcta acgggatgcc aacggacatg accgcactgg aaccgacact cacgcttgca 840

cagacctgcg gcgccctggc caccacagcc aaaggtgcga tgaccgcctt gccttatcag 900

cgcgatctca accgtcagtt ttaatcctta aagccgcttt gcgcggctca ctttgttgca 960

tgcatcacat ttattaaacc ggtttagcat atttgtttta agaaaaacaa aggtcgggct 1020

taacatagcg cctaaaccgg tttagcaaaa attataattt tccattttta cttttgggat 1080

gccaacagca tgtacagaaa aagcacactt gcgatgctta tcgctttgct aaccagcgct 1140

gcctcagccc atgcgcaaac ggatataagc accattgaag cccgactcaa cgcgctggaa 1200

aaacgcctgc aggaggcaga aaacagggcg caaacggcgg aaaaccgcgc cggggcggcg 1260

gagaaaaaag ttcagcaact caccgcgcag cagcaaaaaa accagaactc gactcaggaa 1320

gtggctcagc gtaccgccag acttgagaaa aaagccgatg acaaaagcgg atttgagttt 1380

cacggttacg cccgctccgg cgtgataatg aatgattccg gcgccagcac caaatccgga 1440

gcctacataa cgccggcagg tgaaaccggc ggagctatcg gccgtctggg aaaccaggcc 1500

gatacctatg ttgaaatgaa tcttgaacat aagcagaccc tggataatgg ggccacgacc 1560

cgctttaagg tgatggtcgc cgacgggcaa acctcttata acgactggac tgcaagcacc 1620

agcgatctga acgttcgtca ggcctttgtc gaattgggta acctgccgac gttcgctggg 1680

ccatttaagg gctccaccct gtgggccggg aaacgtttcg accgcgacaa tttcgatatt 1740

cactggattg actctgatgt cgtgttcctc gccggtaccg gtggtggtat ctatgacgtg 1800

aagtggaacg acggcctgcg gagtaatttc tccctgtacg ggcgtaactt cggcgacatt 1860

gatgattcca gcaacagcgt gcagaactat atcctcacca tgaatcactt cgcaggtccg 1920

ctgcagatga tggtcagcgg tctgcgggcg aaggataacg acgagcgtaa agatagcaac 1980

ggcaatctgg caaaaggcga tgcggcaaac accggcgtgc atgcgctgct cggcctgcat 2040

aacgacagtt tctacggcct gcgcgacggt agcagtaaaa ccgctctgct ttatggtcat 2100

ggtctgggcg cagaggttaa aggtatcgga tctgatggcg cacttcgtcc gggagccgac 2160

acatggcgca ttgccagtta cggcaccacg ccgctcagcg aaaactggtc tgttgccccg 2220

gcaatgctgg cgcaacgcag taaagaccgc tatgccgatg gcgacagcta tcagtgggca 2280

acattcaacc tgcgtctgat tcaggcaatc aatcagaatt tcgctctcgc ctacgaaggc 2340

agctaccagt acatggatct taaacccgaa ggttataacg atcgtcaggc ggtgaacggt 2400

agcttctaca agctcacctt cgccccgaca tttaaggtcg gcagtatcgg tgatttcttc 2460

agtcgcccgg agattcgttt ctatacctcc tggatggact ggagcaaaaa actgaataat 2520

tacgccagcg acgacgccct gggcagtgac ggttttaact cgggcggcga atggtctttc 2580

ggtgtgcaga tggaaacctg gttctgacgc ttacgcctga tgacaggaat agccgggggt 2640

cagagcatct ttgtcacccc ggactcaact aagacgcaga aaaagcgctc ccgtgaacgc 2700

gggacgacaa cataaaaatg tttaagcctt aagagggtac tatggatttt gaacagattt 2760

cctgctcgct gcttccgctt cttggaggca aagaaaatat cgccagcgcc gcgcactgcg 2820

ccacgcgcct gcgcctggtg ctggtcgatg attcgctggc cgaccagcag gccatcggca 2880

aagttgaagg ggtgaagggc tgttttcgta atgccggaca gatgcagatt attttcggca 2940

ccggggtggt aaataaggtc tacgctgcct ttactcaggc ggcgggtatt agcgaatcca 3000

gcaaatcgga agccgccgac atcgcggcaa aaaagctcaa tccgttccag cgcatcgccc 3060

gcctgctatc aaacatcttc gtgccgataa tccctgccat cgtcgcctct ggtctgctga 3120

tgggcctgct gggaatggtc aaaacatacg gctgggttga cccgggcaac gccatctaca 3180

tcatgctgga tatgtgcagc tcggcggcat ttatcattct gccgattctg attggcttta 3240

ccgccgcccg cgaattcggc ggtaatcctt atctcggcgc gacgcttggc ggcattctga 3300

ctcatccagc gctgactaac gcctggggcg tggccgcggg tttccacacc atgaactttt 3360

tcggcttcga aattgccatg atcggctatc agggtacggt gttcccggta ctgctggcag 3420

tatggtttat gagcatcgtt gagaagcagt tgcgtcgcgc aatccccgat gccctggatt 3480

tgatcctgac gccgttcctg acggtgatta tatccggttt tatcgccctg ttgattatcg 3540

gcccggccgg tcgcgcactg ggcgacggta tctcgtttgt cctcagcacc ctgattagcc 3600

acgccggctg gctcgccggg ttactgtttg gcggtctcta ttcagttatc gtcattaccg 3660

gtattcatca cagcttccat gcggttgaag ccgggttgct gggcaatccc tccatcggcg 3720

tcaacttcct gctgccgatt tgggcgatgg ccaacgtcgc tcagggcgga gcctgtctgg 3780

cggtgtggtt caaaaccaaa gatgcaaaaa ttaaagccat tactctgccc tcggcgtttt 3840

ccgccatgct gggcatcacc gaggcggcga tttttggtat taacctgcgc tttgtgaagc 3900

catttattgc ggcgctgatt ggtggtgcgg cgggcggcgc atgggtggta tctgtacacg 3960

tctacatgac cgcggtcggc ttgacagcga tccccggcat ggccatcgtg caggccagtt 4020

cgctgttgaa ctacattatc gggatggtta tcgcctttgg cgtcgccttt acggtctccc 4080

tggttttgaa atacaaaacg gacgctgaat aatgtctctt ccatcacgac tgcctgcgat 4140

tttgcaggcc gtaatgcagg gccagccgcg cgcgctggcc gatagccact atccgcgctg 4200

gcaccatgcg ccggtcaccg ggctgatgaa cgaccccaac ggctttatcg aatttgccgg 4260

acgctatcat ctgttttatc agtggaaccc gctcgcctgc gatcatacgt ttaagtgctg 4320

ggcgcactgg agttccatcg atctgctgca ctggcagcat gagcccattg cgctgatgcc 4380

ggacgaagag tatgaccgta acggctgcta ctccggcagc gcggtggata acaacggtac 4440

gcttaccctg tgctataccg gcaacgtgaa gtttgccgag ggagggcgaa ccgcctggca 4500

atgcctggca acggaaaacg ctgacggcac cttccgcaaa atcggtccgg tcctgccgct 4560

gccggagggc tacaccggcc acgtgcgcga cccaaaagtc tggcgacacg aagacctgtg 4620

gtacatggtg ctgggcgcgc aggatcggca aaagcgcggc aaggtgctgc tgttcagctc 4680

tgcggatctc catcagtgga cgagtatggg tgaaatcgcc ggccacggca tcaatggcct 4740

cgacgacgtc ggctatatgt gggagtgccc ggatcttttt ccactcggcg accagcatat 4800

tctaatctgc tgtccgcagg ggattgcccg tgaggaagag tgctacctga acacctaccc 4860

ggcagtatgg atggcgggcg agtttgatta cgctgctggc gctttcagac acggcgaact 4920

gcacgaactg gacgccgggt ttgagttcta cgccccgcaa accatgctta ccagtgatgg 4980

ccgtcgtctg ctggtcggct ggatgggcgt gccggagggc gaagagatgc ttcagccgac 5040

cctgaacaac ggctggatcc atcagatgac ctgcctgcgt gagctggagt ttatcaacgg 5100

tcagctctat cagcgtccgc tacgggaact gagcgccctg cgcggtgaag cgaacggctg 5160

gtcggggaac gccctgccgc tggcaccgat ggaaatcgat ttgcaaaccc gcgggggcga 5220

tatgttgagc ctcgattttg gcggcgtatt aacccttgag tgcgatgcca gcggactccg 5280

cctggcccga cgcagtctcg ccagtgacga gatgcattat cgttactggc gcggaaacgt 5340

ccgctcgctg cgtgttttca tcgaccagtc gagcgtggag attttcataa acggcggtga 5400

aggggtgatg agcagccgct acttcccggc ctgctccggt cagctaacat tctccggcat 5460

cacgccggac gcattctgct actggccgct gcgaacttgc atggtagaat aagcgttttg 5520

cttcaggctc atggcgtcgt aatgaaaacc aaacgcgtaa ccattaaaga tatagccgaa 5580

caggctggcg tctccaaagc gaccgccagc ttggtactga atggtcgtgg caaggagctg 5640

cgcgtggcgc aggaaacgcg tgagcgcgta ctgtcgattg cccgtaagca tcactatcag 5700

ccaagcattc atgcccgctc gctgcgcaac aaccgcagcc acaccatcgg gctggtggtg 5760

ccggagatca ccaaccacgg ctttgcggtc tttgcccatg agctggagat gctgtgccgc 5820

gaggcgggcg tccagctgtt gatctcttgt actgatgaaa accccggtca ggagagcgtg 5880

gtggtcaata atatgattgc ccgccaggtc gacgggatga tcgtcgcttc ctgtatgcac 5940

aacgatgccg actatctcaa actcagccaa cagctgccag tggtgctgtt tgaccggtgc 6000

cccaatgaaa gcgcgctgcc gctggtaatg accgattcga ttaccccaac ggcggaactg 6060

atttcccgca tcgcgcctca gcatagcgat gagttctggt ttttaggcgg tcaggcgcgt 6120

ctgtcgccct cccgcgatcg tctgaccggg ttcacgcagg gtttggctca ggcgggtatt 6180

gccctgcgcc cggaatgggt gatcaacggc aattaccacc ccagctccgg ctatgagatg 6240

tttgccgcac tctgcgcgcg ccttgggcgg ccgcctaagg cgctattcac cgccgcctgc 6300

gggctgctcg aaggggttct gcgctatatg agccagcacc atttactcga ttccgatatt 6360

catctgacga gctttgacga tcactatctt tatgattcgc tgtcgctgcg tatcgacact 6420

gtccagcagg ataatcgcca gctggcctgg cactgctacg atctgataag ccagctgatc 6480

gagggcgata cgcccgaaac gctacaacgc tacctgcccg caaccctgca gtttcggcat 6540

cagtaa 6546

<210> 176

<211> 4885

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 176

ctatattgct gaaggtacag gcgtttccat aactatttgc tcgcgttttt tactcaggaa 60

gaaaatgcca aatagcaaca tcaggcagac aatacccgaa attgcgaaga aaactgtctg 120

gtagcctgcg tggtcaaaga gtatcccagt cggcgttgaa agcagcacaa tcccaagcga 180

actggcaatt tgaaaaccaa tcagaaagat cgtcgacgac aggcgcttat caaagtttgc 240

cacgctgtat ttgaagacgg atatgacaca aagtggaacc tcaatggcat gtaacagctt 300

cactaatgaa ataatccagg ggttaacgaa caacgcgcag gaaaggatac gcaacgccat 360

aatcacaaca ccgataagta atgcattttt tggccctacc cgattcacaa agaaaggaat 420

aatcgccatg cacagcgctt cgagtaccac ctggaatgag ttgagataac catacaggcg 480

cgttcctaca tcgtgtgatt cgaataaacc tgcataaaag acaggaaaga gttgttgatc 540

aaaaatgtta tagaaagacc acgtccccac aataaatatg acgaaaaccc agaagtttcg 600

atccttgaaa actgcgataa aatcctcttt ttttacccct cccgcatccg ccgctatgca 660

ctggtgatcc ttatctttaa aacgcatgtt gatcatcata aatacagcgc caaatagcga 720

gaccaaccag aagttgatat ggggactgat actaaaaaat ataccggcaa agaacgcgcc 780

aatagcatag ccaaaagatc cccaggcgcg cgctgttcca tattcgaaat gaaaatttcg 840

cgccattttt tcggtgaagc tgtcaagcaa accgcatccc gccagatacc ccaggccaaa 900

aaagagcgcc cccagaatta gacctacaga aaaattgctt tgcagtaacg gttcataaac 960

gtaaatcata aacggtccgg tcaagaccag aatgaaactc atacaccaga tgagcggttt 1020

cttcagaccg agtttatcct gaacgatgcc gtagaacatc ataaatagaa tgctggtaaa 1080

ctggttgacc gaataaagtg tacctaattc cgtccctgtt aatcctagat gtcctttcag 1140

ccaaatagcg tataacgacc accacagcga ccaggaaata aaaaagagaa atgagtaact 1200

ggatgcaaaa cgatagtacg catttctgaa tggaatattc agtgccataa ttacctgcct 1260

gtcgttaaaa aattcatgtc ctatttagag ataagagcgg cctcgccgtt tacttctcac 1320

tttccagttc ttgtcgacat ggcagcgctg tcattgcccc tttcgctgtt actgcaagcg 1380

ctccgcaacg ttgagcgaga tcgataattc gtcgcatttc tctctcatct gtagataatc 1440

ccgtagagga cagacctgtg agtaacccgg caacgaacgc atctcccgcc ccagtgctat 1500

cgacacaatt cacagacatt ccagcaaaat ggtggacttg tcctcgataa cagaccacca 1560

ccccttctgc acctttagtc accaacagca tggcgatctc atactctttt gccagggcgc 1620

atatatcccg atcgttctgt gtttttccac tgataagtcg ccattcttct tccgagagct 1680

tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa gcggagcaaa tgctcgtctt 1740

gccatagatc ttcacgaata ttgggatcga agctgacaaa acctccggca tgccggatcg 1800

ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc agacaacgca attgaacaga 1860

gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt cgtctctaaa aaaagatcgg 1920

cactggggcg gaccataaac gtaaatgaac gttctccttg atcgttcaga tcgacaagca 1980

ccgtggatgt ccggtgccat tcatcttgct tcagatacgt gatatcgaca ccctcagtta 2040

gcagcgttct ttgcattaac gcaccaaaag gatcatcacc gacccgacct ataaacccac 2100

ttgttccgcc taatctggcg attcccaccg caacgttagc tggcgcgccg ccaggacaag 2160

gcagtagccg cccgtctgat tctggcaaga gatctacgac cgcatcccct aaaacccata 2220

ctttggctga catttttttc ccttaaattc atctgactta cgcatagtga taaacctctt 2280

tttcgcaaaa tcgtcatgga tttactaaaa catgcatatt cgatcacaaa acgtcatagt 2340

taacgttaac atttgtgata ttcatcgcat ttatgaaagt aagggacttt atttttataa 2400

aagttaacgt taacaattca ccaaatttgc ttaaccagga tgattaaaat gacgcaatct 2460

cgattgcatg cggcgcaaaa cgcactagca aaacttcacg agcgccgagg taacactttc 2520

tatccccatt ttcacctcgc gcctcctgcc gggtggatga acgatccaaa cggcctgatc 2580

tggtttaacg atcgttatca cgcgttttat caacatcacc cgatgagcga acactggggg 2640

ccaatgcact ggggacatgc caccagcgac gatatgatcc actggcagca tgagcctatt 2700

gcgctagcgc caggagacga gaatgacaaa gacgggtgtt tttcaggtag tgctgtcgat 2760

gacaatggtg tcctctcact tatctacacc ggacacgtct ggctcgatgg tgcaggtaat 2820

gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc gggatggtat tcatttcgag 2880

aaacagggtg tgatcctcac tccaccagaa ggcatcatgc acttccgcga tcctaaagtg 2940

tggcgtgaag ccgacacatg gtggatggta gtcggggcga aagacccagg caacacgggg 3000

cagatcctgc tttatcgcgg cagttcattg cgtgaatgga ctttcgatcg cgtactggcc 3060

cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg actttttcag ccttggcgat 3120

cagcattatc tgatgttttc cccgcaggga atgaatgccg agggatacag ttatcgaaat 3180

cgctttcaaa gtggcgtaat acccggaatg tggtcgccag gacgactttt tgcacaatcc 3240

gggcatttta ctgaacttga taacgggcat gacttttatg caccacaaag ctttgtagcg 3300

aaggatggtc ggcgtattgt tatcggctgg atggatatgt gggaatcgcc aatgccctca 3360

aaacgtgaag gctgggcagg ctgcatgacg ctggcgcgcg agctatcaga gagcaatggc 3420

aaactcctac aacgcccggt acacgaagct gagtcgttac gccagcagca tcaatctatc 3480

tctccccgca caatcagcaa taaatatgtt ttgcaggaaa acgcgcaagc agttgagatt 3540

cagttgcagt gggcgctgaa gaacagtgat gccgaacatt acggattaca gctcggcgct 3600

ggaatgcggc tgtatattga taaccaatct gagcgacttg ttttgtggcg gtattaccca 3660

cacgagaatt tagatggcta ccgtagtatt cccctcccgc agggtgacat gctcgcccta 3720

aggatattta tcgatacatc atccgtggaa gtatttatta acgacgggga ggcggtgatg 3780

agtagccgaa tatatccgca gccagaagaa cgggaactgt cgctctatgc ctcccacgga 3840

gtggctgtgc tgcaacatgg agcactctgg caactgggtt aacataatat caggtggaac 3900

aacggatcaa cagcgggcaa gggatccgcg tcactcttcc cccttcacga ccttcaataa 3960

tatgcaatgc agcttcccgc ccgataatgt catgtggaag ctgaattgtg gtcagcggcg 4020

gtaaaaacag atgcccgacg ccaaccagat tatcaaagcc cattacggcg acatcctgcg 4080

ggatacgtac ccccttcgcc aaaagaacct gataagccac aaaggctgcg cgatcgttac 4140

cacatatcag aacatcaaaa tctggtttgc ccgatttgaa gtgggcattg agtaaacttg 4200

cgagatcggt gtagtgatca tcacctgttg ccatgtgaaa ttgtttcacc tcagccagat 4260

ctcgtccagc atcacgccag gcctgctcaa atccctgccg acgataccct gttgccaacg 4320

cactttccgg tagccagaag cataacggtt gacgatagcc cgccgcgagc aaatgctgtg 4380

ttgattcata ttgtgcagtg taatcatcag ggatataact gggtaacgct gggtcatccg 4440

ccacacagtt cgccaataca atattttcac catacagaga ctcaggcagc gtgatatgtc 4500

gcagccccat tgtagtatag ataatgccat ccggacggtg ggcaagcagc tgacgtgccg 4560

cgcgggcagc gtcatcttca gaaaaaatat tgattaaaaa actattccag ccgaactcgc 4620

tggcggtttg ctcaatggca agcagaatat caacagagaa aggagtggta gcagtgtcct 4680

gcgccagcac ggcgagagtc gacggcttac gtccttgagc gcgcatctta cgggcggaaa 4740

gatcaggaac ataattcagg gtctggattg cctgcaatac gcggtcacgc gttgcaggac 4800

gcacagattc tgcattatgc atcacccggg agactgtcat catcgacact cccgccaggc 4860

gtgcgacatc ctttaatgaa gccat 4885

<210> 177

<211> 3771

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 177

atgaaaataa agaacattct actcaccctt tgcacctcac tcctgcttac caacgttgct 60

gcacacgcca aagaagtcaa aataggtatg gcgattgatg atctccgtct tgaacgctgg 120

caaaaagatc gagatatctt tgtgaaaaag gcagaatctc tcggcgcgaa agtatttgta 180

cagtctgcaa atggcaatga agaaacacaa atgtcgcaga ttgaaaacat gataaaccgg 240

ggtgtcgatg ttcttgtcat tattccgtat aacggtcagg tattaagtaa cgttgtaaaa 300

gaagccaaac aagaaggcat taaagtatta gcttacgacc gtatgattaa cgatgcggat 360

atcgattttt atatttcttt cgataacgaa aaagtcggtg aactgcaggc aaaagccctg 420

gtcgatattg ttccgcaagg taattacttc ctgatgggcg gctcgccggt agataacaac 480

gccaagctgt tccgcgccgg acaaatgaaa gtgttaaaac cttacgttga ttccggaaaa 540

attaaagtcg ttggtgacca atgggttgat ggctggttac cggaaaacgc attgaaaatt 600

atggaaaacg cgctaaccgc caataataac aaaattgatg ctgtagttgc ctcaaacgat 660

gccaccgcag gtggggcaat tcaggcatta agcgcgcaag gtttatcagg gaaagtagca 720

atctccggcc aggatgcgga tctcgcaggt attaaacgta ttgctgccgg tacgcaaact 780

atgacggtgt ataaacctat tacgttgttg gcaaatactg ccgcagaaat tgccgttgag 840

ttgggcaatg gtcaggaacc aaaagcagat accacactga ataatggcct gaaagatgtc 900

ccctcccgcc tcctgacacc gatcgatgtg aataaaaaca acatcaaaga tacggtaatt 960

aaagacggat tccacaaaga gagcgagctg taagcgttac gccccagcgc ggagcggggg 1020

cgtgatttct ctccatgccg cgtgaatgaa ttggcttagg tggagtcgtt atgccttatc 1080

tacttgaaat gaagaacatt accaaaacct tcggcagtgt gaaggcgatt gataacgtct 1140

gcttgcggtt gaatgctggc gaaatcgtct cactttgtgg ggaaaatggg tctggtaaat 1200

caacgctgat gaaagtgctg tgtggtattt atccccatgg ctcctacgaa ggcgaaatta 1260

tttttgcggg agaagagatt caggcgagtc acatccgcga taccgaacgc aaaggtatcg 1320

ccatcattca tcaggaattg gccctggtga aagaattgac cgtgctggaa aatatcttcc 1380

tgggtaacga aataacccac aatggcatta tggattatga cctgatgacg ctacgctgtc 1440

agaagctgct cgcacaggtc agtttatcca tttcacctga tacccgcgtt ggcgatttag 1500

ggcttgggca acaacaactg gttgaaattg ccaaggcact taataaacag gtgcgcttgt 1560

taattctcga tgaaccgaca gcctcattaa ctgagcagga aacgtcgatt ttactggata 1620

ttattcgcga tctacaacag cacggtatcg cctgtattta tatttcgcac aaactcaacg 1680

aagtcaaagc gatttccgat acgatttgcg ttattcgcga cggacagcac attggtacgc 1740

gtgatgctgc cggaatgagt gaagacgata ttatcaccat gatggtcggg cgagagttaa 1800

ccgcgcttta ccctaatgaa ccacatacca ccggagatga aatattacgt attgaacatc 1860

tgacggcatg gcatccggtt aatcgtcata ttaaacgagt taatgatgtc tcgttttccc 1920

tgaaacgtgg cgaaatattg ggtattgccg gactcgttgg tgccggacgt accgagacca 1980

ttcagtgcct gtttggtgtg tggcccggac aatgggaagg aaaaatttat attgatggca 2040

aacaggtaga tattcgtaac tgtcagcaag ccatcgccca ggggattgcg atggtccccg 2100

aagacagaaa gcgcgacggc atcgttccgg taatggcggt tggtaaaaat attaccctcg 2160

ccgcactcaa taaatttacc ggtggcatta gccagcttga tgacgcggca gagcaaaaat 2220

gtattctgga atcaatccag caactcaaag ttaaaacgtc gtcccccgac cttgctattg 2280

gacgtttgag cggcggcaat cagcaaaaag cgatcctcgc tcgctgtctg ttacttaacc 2340

cgcgcattct cattcttgat gaacccacca ggggtatcga tattggcgcg aaatacgaga 2400

tctacaaatt aattaaccaa ctcgtccagc agggtattgc cgttattgtc atctcttccg 2460

aattacctga agtgctcggc cttagcgatc gtgtactggt gatgcatgaa gggaaactaa 2520

aagccaacct gataaatcat aacctgactc aggagcaggt gatggaagcc gcattgagga 2580

gcgaacatca tgtcgaaaag caatccgtct gaagtgaaat tggccgtacc gacatccggt 2640

ggcttctccg ggctgaaatc actgaatttg caggtcttcg tgatgattgc agctatcatc 2700

gcaatcatgc tgttctttac ctggaccacc gatggtgcct acttaagcgc ccgtaacgtc 2760

tccaacctgt tacgccagac cgcgattacc ggcatcctcg cggtaggaat ggtgttcgtc 2820

ataatttctg ctgaaatcga cctttccgtc ggctcaatga tggggctgtt aggtggcgtc 2880

gcggcgattt gtgacgtctg gttaggctgg cctttgccac ttaccatcat tgtgacgctg 2940

gttctgggac tgcttctcgg tgcctggaac ggatggtggg tcgcgtaccg taaagtccct 3000

tcatttattg tcaccctcgc gggcatgttg gcatttcgcg gcatactcat tggcatcacc 3060

aacggcacga ctgtatcccc caccagcgcc gcgatgtcac aaattgggca aagctatctc 3120

cccgccagta ccggcttcat cattggcgcg cttggcttaa tggcttttgt tggttggcaa 3180

tggcgcggaa gaatgcgccg tcaggctttg ggtttacagt ctccggcctc taccgcagta 3240

gtcggtcgcc aggctttaac cgctatcatc gtattaggcg caatctggct gttgaatgat 3300

taccgtggcg ttcccactcc tgttctgctg ctgacgttgc tgttactcgg cggaatgttt 3360

atggcaacgc ggacggcatt tggacgacgc atttatgcca tcggcggcaa tctggaagca 3420

gcacgtctct ccgggattaa cgttgaacgc accaaacttg ccgtgttcgc gattaacgga 3480

ttaatggtag ccatcgccgg attaatcctt agttctcgac ttggcgctgg ttcaccttct 3540

gcgggaaata tcgccgaact ggacgcaatt gcagcatgcg tgattggcgg caccagcctg 3600

gctggcggtg tgggaagcgt tgccggagca gtaatggggg catttatcat ggcttcactg 3660

gataacggca tgagtatgat ggatgtaccg accttctggc agtatatcgt taaaggtgcg 3720

attctgttgc tggcagtatg gatggactcc gcaaccaaac gccgttcttg a 3771

<210> 178

<211> 1395

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 178

atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc 60

cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc tggcgcactg 120

ccgtttattg cagatgaatt ccagattact tcgcacacgc aagaatgggt cgtaagctcc 180

atgatgttcg gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt taaactcggg 240

cgcaaaaaga gcctgatgat cggcgcaatt ttgtttgttg ccggttcgct gttctctgcg 300

gctgcgccaa acgttgaagt actgattctt tcccgcgttc tactggggct ggcggtgggt 360

gtggcctctt ataccgcacc gctgtacctc tctgaaattg cgccggaaaa aattcgtggc 420

agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc ttatctttct 480

gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg 540

gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc 600

aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa 660

gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag tggctgggcg 720

ctgtttaaag agaacagcaa cttccgccgc gcggtgttcc ttggcgtact gttgcaggta 780

atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat cttcgaactg 840

gcgggttata ccaacactac cgagcaaatg tgggggaccg tgattgtcgg cctgaccaac 900

gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa accaacgcta 960

acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat gatgcatatc 1020

ggtattcact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat gtttattgtc 1080

ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat tcagccgctg 1140

aaaggccgcg attttggcat cacctgctcc actgccacca actggattgc caacatgatc 1200

gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac cttctgggtg 1260

tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa 1320

cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaata 1380

ggcgctcacg attaa 1395

<210> 179

<211> 1422

<212> DNA

<213> 运动发酵单胞菌(Zymomonas mobilis)

<400> 179

atgagttctg aaagtagtca gggtctagtc acgcgactag ccctaatcgc tgctataggc 60

ggcttgcttt tcggttacga ttcagcggtt atcgctgcaa tcggtacacc ggttgatatc 120

cattttattg cccctcgtca cctgtctgct acggctgcgg cttccctttc tgggatggtc 180

gttgttgctg ttttggtcgg ttgtgttacc ggttctttgc tgtctggctg gattggtatt 240

cgcttcggtc gtcgcggcgg attgttgatg agttccattt gtttcgtcgc cgccggtttt 300

ggtgctgcgt taaccgaaaa attatttgga accggtggtt cggctttaca aattttttgc 360

tttttccggt ttcttgccgg tttaggtatc ggtgtcgttt caaccttgac cccaacctat 420

attgctgaaa ttcgtccgcc agacaaacgt ggtcagatgg tttctggtca gcagatggcc 480

attgtgacgg gtgctttaac cggttatatc tttacctggt tactggctca tttcggttct 540

atcgattggg ttaatgccag tggttggtgc tggtctccgg cttcagaagg cctgatcggt 600

attgccttct tattgctgct gttaaccgca ccggatacgc cgcattggtt ggtgatgaag 660

ggacgtcatt ccgaggctag caaaatcctt gctcgtctgg aaccgcaagc cgatcctaat 720

ctgacgattc aaaagattaa agctggcttt gataaagcca tggacaaaag cagcgcaggt 780

ttgtttgctt ttggtatcac cgttgttttt gccggtgtat ccgttgctgc cttccagcag 840

ttagtcggta ttaacgccgt gctgtattat gcaccgcaga tgttccagaa tttaggtttt 900

ggagctgata cggcattatt gcagaccatc tctatcggtg ttgtgaactt catcttcacc 960

atgattgctt cccgtgttgt tgaccgcttc ggccgtaaac ctctgcttat ttggggtgct 1020

ctcggtatgg ctgcaatgat ggctgtttta ggctgctgtt tctggttcaa agtcggtggt 1080

gttttgcctt tggcttctgt gcttctttat attgcagtct ttggtatgtc atggggccct 1140

gtctgctggg ttgttctgtc agaaatgttc ccgagttcca tcaagggcgc agctatgcct 1200

atcgctgtta ccggacaatg gttagctaat atcttggtta acttcctgtt taaggttgcc 1260

gatggttctc cagcattgaa tcagactttc aaccacggtt tctcctatct cgttttcgca 1320

gcattaagta tcttaggtgg cttgattgtt gctcgcttcg tgccggaaac caaaggtcgg 1380

agcctggatg aaatcgagga gatgtggcgc tcccagaagt ag 1422

<210> 180

<211> 984

<212> DNA

<213> 运动发酵单胞菌(Zymomonas mobilis)

<400> 180

atggaaattg ttgcgattga catcggtgga acgcatgcgc gtttctctat tgcggaagta 60

agcaatggtc gggttctttc tcttggagaa gaaacaactt ttaaaacggc agaacatgct 120

agcttgcagt tagcttggga acgtttcggt gaaaaactgg gtcgtcctct gccacgtgcc 180

gcagctattg catgggctgg cccggttcat ggtgaagttt taaaacttac caataaccct 240

tgggtattaa gaccagctac tctgaatgaa aagctggaca tcgatacgca tgttctgatc 300

aatgacttcg gcgcggttgc ccacgcggtt gcgcatatgg attcttctta tctggatcat 360

atttgtggtc ctgatgaagc gcttcctagc gatggtgtta tcactattct tggtccggga 420

acgggcttgg gtgttgccca tctgttgcgg actgaaggcc gttatttcgt catcgaaact 480

gaaggcggtc atatcgactt tgctccgctt gacagacttg aagacaaaat tctggcacgt 540

ttacgtgaac gtttccgccg cgtttctatc gaacgcatta tttctggccc gggtcttggt 600

aatatctacg aagcactggc tgccattgaa ggcgttccgt tcagcttgct ggatgatatt 660

aaattatggc agatggcttt ggaaggtaaa gacaaccttg ctgaagccgc tttggatcgc 720

ttctgcttga gccttggcgc tatcgctggt gatcttgctt tggcacaggg tcgaaccagt 780

gttgttattg gcggtggtgt cggtcttcgt atcgcttccc atttgccaga atctggtttc 840

cgtcagcgct ttgtttcaaa aggacgcttt gaacgcgtca tgtccaagat tccggttaag 900

ttgattactt atccgcagcc tggactgttg ggtgcgcagc tgcctatgcc aacaaatatt 960

ctgaagttga ataatatttt ttaa 984

<210> 181

<211> 966

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 181

atgacaaagt atgcattagt cggtgatgtg ggcggcacca acgcacgtct tgctctgtgt 60

gatattgcca gtggtgaaat ctcgcaggct aagacctatt cagggcttga ttaccccagc 120

ctcgaagcgg tcattcgcgt ttatcttgaa gaacataagg tcgaggtgaa agacggctgt 180

attgccatcg cttgcccaat taccggtgac tgggtggcga tgaccaacca tacctgggcg 240

ttctcaattg ccgaaatgaa aaagaatctc ggttttagcc atctggaaat tattaacgat 300

tttaccgctg tatcgatggc gatcccgatg ctgaaaaaag agcatctgat tcagtttggt 360

ggcgcagaac cggtcgaagg taagcctatt gcggtttacg gtgccggaac ggggcttggg 420

gttgcgcatc tggtccatgt cgataagcgt tgggtaagct tgccaggcga aggcggtcac 480

gttgattttg cgccgaatag tgaagaagag gccattatcc tcgaaatatt gcgtgcggaa 540

attggtcatg tttcggcgga gcgcgtgctt tctggccctg ggctggtgaa tttgtatcgc 600

gcaattgtga aagctgacaa ccgcctgcca gaaaatctca agccaaaaga tattaccgaa 660

cgcgcgctgg ctgacagctg caccgattgc cgccgcgcat tgtcgctgtt ttgcgtcatt 720

atgggccgtt ttggcggcaa tctggcgctc aatctcggga catttggcgg cgtgtttatt 780

gcgggcggta tcgtgccgcg cttccttgag ttcttcaaag cctccggttt ccgtgccgca 840

tttgaagata aagggcgctt taaagaatat gtccatgata ttccggtgta tctcatcgtc 900

catgacaatc cgggccttct cggttccggt gcacatttac gccagacctt aggtcacatt 960

ctgtaa 966

<210> 182

<211> 2547

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 182

ttgtacctct atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg 60

gatcgcgcgc ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca 120

ttgttgcact atcaccatcc gctaatgccg ggttaccttg atggtaacgt tcccaaaggc 180

atttgccttt acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat 240

cgtggaatgt cagtacagga tccgccgaaa ggtgagcttc caattactgg tgtatacacc 300

atgggcagca cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt 360

catcaatcct ggctcgatag cgaagagcgc caattgctac aacgtaaatg tagcctgctg 420

gaaaactggg ccgcctcgct gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc 480

ttccgtcata atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata 540

ctgctgcttg acgaatttta tcgtaccgcc gtgcgtctcg ccggtaagcg tattctgtgg 600

aatatggtgc cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg 660

cagggcgtgc tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct 720

gaagagtact ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa 780

gcggtactga aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg 840

ctggcgaaag atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggtctcgat 900

ccatactgca tgatgctgga gcgtgttact gaatacctga cggcgattga agattttacc 960

cgtctggatt tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaagct cagccgtgaa 1020

cgcgcctgcg taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg 1080

gacgaagctc gtctggcaat gctcgataac cgcgctaact ggaagattga tcaggtgcgt 1140

gaggcgcaca acgagttgct cgacgcgatg atgcagagct accgtaatct gatccgcttt 1200

gcgcgtcgca ataaccttag cgtctccgcc agtccgcagg atatcggcgt gctgacgcgt 1260

aagctgtatg ccgcgtttga agcattacca ggtaaagtga cgctggtaaa cccgcagatt 1320

tcacccgatc tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgggctaac 1380

cgttcaggtt ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag 1440

ccgctggaat ataaccgtta cctgaataaa ctggtggcgt gggcatggtt taacggcctg 1500

ctgacctcgc gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg 1560

caggagatgg tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg 1620

aaggcgctct acagcccgtg tgagatccgc catctggcga ttatcgttaa cctggaatat 1680

gacccgacag cggcgttccg caatcaggtg gtgcatttcg atttccgtaa gctggatgtc 1740

ttcagctttg gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac 1800

tcgtggaacg aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg 1860

aaaactattc tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc 1920

tgttatagcc agcatctgcg cggcttaatt cgtactcgcg tgcagcaact ggtttctgag 1980

tgtattgaat tgcgtctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc 2040

gtttctggtc aaacctgggg gttgttcttc gaacgcctga atgtatcggt acagaaactg 2100

gaaaacgcca tcgagtttta tggcgcgatt tcgcataaca aactgcacgg cctgtcagtg 2160

caggttgaaa ccaatcacgt caaattaccg gcggtggtgg acggctttgc cagcgaaggg 2220

atcatccagt tctttttcga agaaacgcaa gacgagaatg gctttaatat ctacattctc 2280

gacgaaagca accgggttga ggtatatcac cactgcgaag gcagcaaaga ggagctggta 2340

cgtgacgtca gtcgcttcta ctcgtcatcg catgaccgtt ttacctacgg ctcaagcttc 2400

atcaacttca acctgccgca gttctatcag attgtgaagg ttgatggtcg tgaacaggtg 2460

attccgttcc gcacaaaatc tatcggtaac atgccgcctg ccaatcagga tcacgatacg 2520

ccgctattac agcaatattt ttcgtga 2547

<210> 183

<211> 210

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 183

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys Asp Glu Glu Gly Lys Glu Met Ile Leu Ser Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Gly Arg Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 184

<211> 210

<212> PRT

<213> 人工的

<220>

<223> Crp*突变体

<400> 184

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys His Glu Glu Gly Lys Glu Met Ile Leu Ser Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Gly Arg Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 185

<211> 210

<212> PRT

<213> 人工的

<220>

<223> Crp*突变体

<400> 185

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys Asp Glu Glu Gly Lys Glu Met Ile Leu Phe Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Gly Arg Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 186

<211> 210

<212> PRT

<213> 人工的

<220>

<223> Crp*突变体

<400> 186

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys Asp Glu Glu Gly Lys Glu Met Ile Leu Ser Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Asp Arg Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 187

<211> 210

<212> PRT

<213> 人工的

<220>

<223> Crp*突变体

<400> 187

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys Asp Glu Glu Gly Lys Glu Met Ile Leu Ser Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Gly Asp Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 188

<211> 2932

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 188

atgcgaattg gcataccaag agaacggtta accaatgaaa cccgtgttgc agcaacgcca 60

aaaacagtgg aacagctgct gaaactgggt tttaccgtcg cggtagagag cggcgcgggt 120

caactggcaa gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg 180

aatagcgtct ggcagtcaga gatcattctg aaggtcaatg cgccgttaga tgatgaaatt 240

gcgttactga atcctgggac aacgctggtg agttttatct ggcctgcgca gaatccggaa 300

ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt 360

atctcacgcg cacaatcgct ggacgcacta agctcgatgg cgaacatcgc cggttatcgc 420

gccattgttg aagcggcaca tgaatttggg cgcttcttta ccgggcaaat tactgcggcc 480

gggaaagtgc caccggcaaa agtgatggtg attggtgcgg gtgttgcagg tctggccgcc 540

attggcgcag caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg 600

aaagaacaag ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa agaggaagct 660

ggcagcggcg atggctatgc caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa 720

ctctttgccg cccaggcaaa agaggtcgat atcattgtca ccaccgcgct tattccaggc 780

aaaccagcgc cgaagctaat tacccgtgaa atggttgact ccatgaaggc gggcagtgtg 840

attgtcgacc tggcagccca aaacggcggc aactgtgaat acaccgtgcc gggtgaaatc 900

ttcactacgg aaaatggtgt caaagtgatt ggttataccg atcttccggg ccgtctgccg 960

acgcaatcct cacagcttta cggcacaaac ctcgttaatc tgctgaaact gttgtgcaaa 1020

gagaaagacg gcaatatcac tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg 1080

atccgtgcgg gcgaaattac ctggccggca ccgccgattc aggtatcagc tcagccgcag 1140

gcggcacaaa aagcggcacc ggaagtgaaa actgaggaaa aatgtacctg ctcaccgtgg 1200

cgtaaatacg cgttgatggc gctggcaatc attctttttg gctggatggc aagcgttgcg 1260

ccgaaagaat tccttgggca cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac 1320

gtggtgtgga atgtatcgca cgcgctgcat acaccgttga tgtcggtcac caacgcgatt 1380

tcagggatta ttgttgtcgg agcactgttg cagattggcc agggcggctg ggttagcttc 1440

cttagtttta tcgcggtgct tatagccagc attaatattt tcggtggctt caccgtgact 1500

cagcgcatgc tgaaaatgtt ccgcaaaaat taaggggtaa catatgtctg gaggattagt 1560

tacagctgca tacattgttg ccgcgatcct gtttatcttc agtctggccg gtctttcgaa 1620

acatgaaacg tctcgccagg gtaacaactt cggtatcgcc gggatggcga ttgcgttaat 1680

cgcaaccatt tttggaccgg atacgggtaa tgttggctgg atcttgctgg cgatggtcat 1740

tggtggggca attggtatcc gtctggcgaa gaaagttgaa atgaccgaaa tgccagaact 1800

ggtggcgatc ctgcatagct tcgtgggtct ggcggcagtg ctggttggct ttaacagcta 1860

tctgcatcat gacgcgggaa tggcaccgat tctggtcaat attcacctga cggaagtgtt 1920

cctcggtatc ttcatcgggg cggtaacgtt cacgggttcg gtggtggcgt tcggcaaact 1980

gtgtggcaag atttcgtcta aaccattgat gctgccaaac cgtcacaaaa tgaacctggc 2040

ggctctggtc gtttccttcc tgctgctgat tgtatttgtt cgcacggaca gcgtcggcct 2100

gcaagtgctg gcattgctga taatgaccgc aattgcgctg gtattcggct ggcatttagt 2160

cgcctccatc ggtggtgcag atatgccagt ggtggtgtcg atgctgaact cgtactccgg 2220

ctgggcggct gcggctgcgg gctttatgct cagcaacgac ctgctgattg tgaccggtgc 2280

gctggtcggt tcttcggggg ctatcctttc ttacattatg tgtaaggcga tgaaccgttc 2340

ctttatcagc gttattgcgg gtggtttcgg caccgacggc tcttctactg gcgatgatca 2400

ggaagtgggt gagcaccgcg aaatcaccgc agaagagaca gcggaactgc tgaaaaactc 2460

ccattcagtg atcattactc cggggtacgg catggcagtc gcgcaggcgc aatatcctgt 2520

cgctgaaatt actgagaaat tgcgcgctcg tggtattaat gtgcgtttcg gtatccaccc 2580

ggtcgcgggg cgtttgcctg gacatatgaa cgtattgctg gctgaagcaa aagtaccgta 2640

tgacatcgtg ctggaaatgg acgagatcaa tgatgacttt gctgataccg ataccgtact 2700

ggtgattggt gctaacgata cggttaaccc ggcggcgcag gatgatccga agagtccgat 2760

tgctggtatg cctgtgctgg aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc 2820

gatgaacact ggctatgctg gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat 2880

gctgtttggt gacgccaaag ccagcgtgga tgcaatcctg aaagctctgt aa 2932

<210> 189

<211> 1401

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 189

atgccacatt cctacgatta cgatgccata gtaataggtt ccggccccgg cggcgaaggc 60

gctgcaatgg gcctggttaa gcaaggtgcg cgcgtcgcag ttatcgagcg ttatcaaaat 120

gttggcggcg gttgcaccca ctggggcacc atcccgtcga aagctctccg tcacgccgtc 180

agccgcatta tagaattcaa tcaaaaccca ctttacagcg accattcccg actgctccgc 240

tcttcttttg ccgatatcct taaccatgcc gataacgtga ttaatcaaca aacgcgcatg 300

cgtcagggat tttacgaacg taatcactgt gaaatattgc agggaaacgc tcgctttgtt 360

gacgagcata cgttggcgct ggattgcccg gacggcagcg ttgaaacact aaccgctgaa 420

aaatttgtta ttgcctgcgg ctctcgtcca tatcatccaa cagatgttga tttcacccat 480

ccacgcattt acgacagcga ctcaattctc agcatgcacc acgaaccgcg ccatgtactt 540

atctatggtg ctggagtgat cggctgtgaa tatgcgtcga tcttccgcgg tatggatgta 600

aaagtggatc tgatcaacac ccgcgatcgc ctgctggcat ttctcgatca agagatgtca 660

gattctctct cctatcactt ctggaacagt ggcgtagtga ttcgtcacaa cgaagagtac 720

gagaagatcg aaggctgtga cgatggtgtg atcatgcatc tgaagtcggg taaaaaactg 780

aaagctgact gcctgctcta tgccaacggt cgcaccggta ataccgattc gctggcgtta 840

cagaacattg ggctagaaac tgacagccgc ggacagctga aggtcaacag catgtatcag 900

accgcacagc cacacgttta cgcggtgggc gacgtgattg gttatccgag cctggcgtcg 960

gcggcctatg accaggggcg cattgccgcg caggcgctgg taaaaggcga agccaccgca 1020

catctgattg aagatatccc taccggtatt tacaccatcc cggaaatcag ctctgtgggc 1080

aaaaccgaac agcagctgac cgcaatgaaa gtgccatatg aagtgggccg cgcccagttt 1140

aaacatctgg cacgcgcaca aatcgtcggc atgaacgtgg gcacgctgaa aattttgttc 1200

catcgggaaa caaaagagat tctgggtatt cactgctttg gcgagcgcgc tgccgaaatt 1260

attcatatcg gtcaggcgat tatggaacag aaaggtggcg gcaacactat tgagtacttc 1320

gtcaacacca cctttaacta cccgacgatg gcggaagcct atcgggtagc tgcgttaaac 1380

ggtttaaacc gcctgtttta a 1401

<210> 190

<211> 1434

<212> DNA

<213> 突变链球菌(Streptococcus mutans)

<400> 190

atggcaatga caaaacaata taaaaattat gtcaatggcg agtggaagct ttcagaaaat 60

gaaattaaaa tctacgaacc agccagtgga gctgaattgg gttcagttcc agcaatgagt 120

actgaagaag tagattatgt ttatgcttca gccaagaaag ctcaaccagc ttggcgagca 180

ctttcataca tagaacgtgc tgcctacctt cataaggtag cagatatttt gatgcgtgat 240

aaagaaaaaa taggtgctat tctttccaaa gaggttgcta aaggttataa atcagcagtc 300

agcgaagttg ttcgtactgc agaaatcatt aattatgcag ctgaagaagg tcttcgtatg 360

gaaggtgaag tccttgaagg cggcagtttt gaagcagcca gcaagaaaaa aattgccgtt 420

gttcgtcgtg aaccagtagg tcttgtatta gctatttcac catttaacta ccctgttaac 480

ttggcaggtt cgaaaattgc accggctctt attgcgggaa atgttattgc ttttaaacca 540

ccgacgcaag gatcaatctc agggctctta cttgctgaag catttgctga agctggactt 600

cctgcaggtg tctttaatac cattacaggt cgtggttctg aaattggaga ctatattgta 660

gaacatcaag ccgttaactt tatcaatttc actggttcaa caggaattgg cgaacgtatt 720

ggcaaaatgg ctggtatgcg tccgattatg cttgaactcg gtggaaaaga ttcagccatc 780

gttcttgaag atgcggacct tgaattgact gctaaaaata ttattgcagg tgcttttggt 840

tattcaggtc aacgctgtac agcagttaaa cgtgttcttg tgatggaaag tgttgctgat 900

gaactggtcg aaaaaatccg tgaaaaagtt cttgcattaa caattggtaa tccagaagac 960

gatgcagata ttacaccgtt gattgataca aaatcagctg attatgtaga aggtcttatt 1020

aatgatgcca atgataaagg agccactgcc cttactgaaa tcaaacgtga aggtaatctt 1080

atctgtccaa tcctctttga taaggtaacg acagatatgc gtcttgcttg ggaagaacca 1140

tttggtcctg ttcttccgat cattcgtgtg acatctgtag aagaagccat tgaaatttct 1200

aacaaatcgg aatatggact tcaggcttct atctttacaa atgatttccc acgcgctttt 1260

ggtattgctg agcagcttga agttggtaca gttcatatca ataataagac acagcgcggc 1320

acggacaact tcccattctt aggggctaaa aaatcaggtg caggtattca aggggtaaaa 1380

tattctattg aagctatgac aactgttaaa tccgtcgtat ttgatatcaa ataa 1434

<210> 191

<211> 996

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 191

atgactatca aagtaggtat caacggtttt ggccgtatcg gtcgcattgt tttccgtgct 60

gctcagaaac gttctgacat cgagatcgtt gcaatcaacg acctgttaga cgctgattac 120

atggcataca tgctgaaata tgactccact cacggccgtt tcgacggtac cgttgaagtg 180

aaagacggtc atctgatcgt taacggtaaa aaaatccgtg ttaccgctga acgtgatccg 240

gctaacctga aatgggacga agttggtgtt gacgttgtcg ctgaagcaac tggtctgttc 300

ctgactgacg aaactgctcg taaacacatc accgctggtg cgaagaaagt ggttatgact 360

ggtccgtcta aagacaacac tccgatgttc gttaaaggcg ctaacttcga caaatatgct 420

ggccaggaca tcgtttccaa cgcttcctgc accaccaact gcctggctcc gctggctaaa 480

gttatcaacg ataacttcgg catcatcgaa ggtctgatga ccaccgttca cgctactacc 540

gctactcaga aaaccgttga tggcccgtct cacaaagact ggcgcggcgg ccgcggcgct 600

tcccagaaca tcatcccgtc ctctaccggt gctgctaaag ctgtaggtaa agtactgcca 660

gaactgaatg gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa cgtatctgta 720

gttgacctga ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa agctgccgtt 780

aaagctgctg ctgaaggcga aatgaaaggc gttctgggct acaccgaaga tgacgtagta 840

tctaccgatt tcaacggcga agtttgcact tccgtgttcg atgctaaagc tggtatcgct 900

ctgaacgaca acttcgtgaa actggtatcc tggtacgaca acgaaaccgg ttactccaac 960

aaagttctgg acctgatcgc tcacatctcc aaataa 996

<210> 192

<211> 1650

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 192

atgaaaaaca tcaatccaac gcagaccgct gcctggcagg cactacagaa acacttcgat 60

gaaatgaaag acgttacgat cgccgatctt tttgctaaag acggcgatcg tttttctaag 120

ttctccgcaa ccttcgacga tcagatgctg gtggattact ccaaaaaccg catcactgaa 180

gagacgctgg cgaaattaca ggatctggcg aaagagtgcg atctggcggg cgcgattaag 240

tcgatgttct ctggcgagaa gatcaaccgc actgaaaacc gcgccgtgct gcacgtagcg 300

ctgcgtaacc gtagcaatac cccgattttg gttgatggca aagacgtaat gccggaagtc 360

aacgcggtgc tggagaagat gaaaaccttc tcagaagcga ttatttccgg tgagtggaaa 420

ggttataccg gcaaagcaat cactgacgta gtgaacatcg ggatcggcgg ttctgacctc 480

ggcccataca tggtgaccga agctctgcgt ccgtacaaaa accacctgaa catgcacttt 540

gtttctaacg tcgatgggac tcacatcgcg gaagtgctga aaaaagtaaa cccggaaacc 600

acgctgttct tggtagcatc taaaaccttc accactcagg aaactatgac caacgcccat 660

agcgcgcgtg actggttcct gaaagcggca ggtgatgaaa aacacgttgc aaaacacttt 720

gcggcgcttt ccaccaatgc caaagccgtt ggcgagtttg gtattgatac tgccaacatg 780

ttcgagttct gggactgggt tggcggccgt tactctttgt ggtcagcgat tggcctgtcg 840

attgttctct ccatcggctt tgataacttc gttgaactgc tttccggcgc acacgcgatg 900

gacaagcatt tctccaccac gcctgccgag aaaaacctgc ctgtactgct ggcgctgatt 960

ggcatctggt acaacaattt ctttggtgcg gaaactgaag cgattctgcc gtatgaccag 1020

tatatgcacc gtttcgcggc gtacttccag cagggcaata tggagtccaa cggtaagtat 1080

gttgaccgta acggtaacgt tgtggattac cagactggcc cgattatctg gggtgaacca 1140

ggcactaacg gtcagcacgc gttctaccag ctgatccacc agggaaccaa aatggtaccg 1200

tgcgatttca tcgctccggc tatcacccat aacccgctct ctgatcatca ccagaaactg 1260

ctgtctaact tcttcgccca gaccgaagcg ctggcgtttg gtaaatcccg cgaagtggtt 1320

gagcaggaat atcgtgatca gggtaaagat ccggcaacgc ttgactacgt ggtgccgttc 1380

aaagtattcg aaggtaaccg cccgaccaac tccatcctgc tgcgtgaaat cactccgttc 1440

agcctgggtg cgttgattgc gctgtatgag cacaaaatct ttactcaggg cgtgatcctg 1500

aacatcttca ccttcgacca gtggggcgtg gaactgggta aacagctggc gaaccgtatt 1560

ctgccagagc tgaaagatga taaagaaatc agcagccacg atagctcgac caatggtctg 1620

attaaccgct ataaagcgtg gcgcggttaa 1650

<210> 193

<211> 963

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 193

atgattaaga aaatcggtgt gttgacaagc ggcggtgatg cgccaggcat gaacgccgca 60

attcgcgggg ttgttcgttc tgcgctgaca gaaggtctgg aagtaatggg tatttatgac 120

ggctatctgg gtctgtatga agaccgtatg gtacagctag accgttacag cgtgtctgac 180

atgatcaacc gtggcggtac gttcctcggt tctgcgcgtt tcccggaatt ccgcgacgag 240

aacatccgcg ccgtggctat cgaaaacctg aaaaaacgtg gtatcgacgc gctggtggtt 300

atcggcggtg acggttccta catgggtgca atgcgtctga ccgaaatggg cttcccgtgc 360

atcggtctgc cgggcactat cgacaacgac atcaaaggca ctgactacac tatcggtttc 420

ttcactgcgc tgagcaccgt tgtagaagcg atcgaccgtc tgcgtgacac ctcttcttct 480

caccagcgta tttccgtggt ggaagtgatg ggccgttatt gtggagatct gacgttggct 540

gcggccattg ccggtggctg tgaattcgtt gtggttccgg aagttgaatt cagccgtgaa 600

gacctggtaa acgaaatcaa agcgggtatc gcgaaaggta aaaaacacgc gatcgtggcg 660

attaccgaac atatgtgtga tgttgacgaa ctggcgcatt tcatcgagaa agaaaccggt 720

cgtgaaaccc gcgcaactgt gctgggccac atccagcgcg gtggttctcc ggtgccttac 780

gaccgtattc tggcttcccg tatgggcgct tacgctatcg atctgctgct ggcaggttac 840

ggcggtcgtt gtgtaggtat ccagaacgaa cagctggttc accacgacat catcgacgct 900

atcgaaaaca tgaagcgtcc gttcaaaggt gactggctgg actgcgcgaa aaaactgtat 960

taa 963

<210> 194

<211> 1476

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 194

atggcggtaa cgcaaacagc ccaggcctgt gacctggtca ttttcggcgc gaaaggcgac 60

cttgcgcgtc gtaaattgct gccttccctg tatcaactgg aaaaagccgg tcagctcaac 120

ccggacaccc ggattatcgg cgtagggcgt gctgactggg ataaagcggc atataccaaa 180

gttgtccgcg aggcgctcga aactttcatg aaagaaacca ttgatgaagg tttatgggac 240

accctgagtg cacgtctgga tttttgtaat ctcgatgtca atgacactgc tgcattcagc 300

cgtctcggcg cgatgctgga tcaaaaaaat cgtatcacca ttaactactt tgccatgccg 360

cccagcactt ttggcgcaat ttgcaaaggg cttggcgagg caaaactgaa tgctaaaccg 420

gcacgcgtag tcatggagaa accgctgggg acgtcgctgg cgacctcgca ggaaatcaat 480

gatcaggttg gcgaatactt cgaggagtgc caggtttacc gtatcgacca ctatcttggt 540

aaagaaacgg tgctgaacct gttggcgctg cgttttgcta actccctgtt tgtgaataac 600

tgggacaatc gcaccattga tcatgttgag attaccgtgg cagaagaagt ggggatcgaa 660

gggcgctggg gctattttga taaagccggt cagatgcgcg acatgatcca gaaccacctg 720

ctgcaaattc tttgcatgat tgcgatgtct ccgccgtctg acctgagcgc agacagcatc 780

cgcgatgaaa aagtgaaagt actgaagtct ctgcgccgca tcgaccgctc caacgtacgc 840

gaaaaaaccg tacgcgggca atatactgcg ggcttcgccc agggcaaaaa agtgccggga 900

tatctggaag aagagggcgc gaacaagagc agcaatacag aaactttcgt ggcgatccgc 960

gtcgacattg ataactggcg ctgggccggt gtgccattct acctgcgtac tggtaaacgt 1020

ctgccgacca aatgttctga agtcgtggtc tatttcaaaa cacctgaact gaatctgttt 1080

aaagaatcgt ggcaggatct gccgcagaat aaactgacta tccgtctgca acctgatgaa 1140

ggcgtggata tccaggtact gaataaagtt cctggccttg accacaaaca taacctgcaa 1200

atcaccaagc tggatctgag ctattcagaa acctttaatc agacgcatct ggcggatgcc 1260

tatgaacgtt tgctgctgga aaccatgcgt ggtattcagg cactgtttgt acgtcgcgac 1320

gaagtggaag aagcctggaa atgggtagac tccattactg aggcgtgggc gatggacaat 1380

gatgcgccga aaccgtatca ggccggaacc tggggacccg ttgcctcggt ggcgatgatt 1440

acccgtgatg gtcgttcctg gaatgagttt gagtaa 1476

<210> 195

<211> 474

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 195

Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro

1 5 10 15

Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr

20 25 30

Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val

35 40 45

Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu

50 55 60

Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys

65 70 75 80

Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln

85 90 95

Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val

100 105 110

Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu

115 120 125

Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala

130 135 140

Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro

145 150 155 160

Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu

165 170 175

Arg Leu Leu Val Met Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Thr

180 185 190

Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Glu Met Phe Asp

195 200 205

Gln Val Ile Pro Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys

210 215 220

Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala

225 230 235 240

Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys

245 250 255

Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly

260 265 270

Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu

275 280 285

Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn

290 295 300

Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu

305 310 315 320

Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala

325 330 335

Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr

340 345 350

Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys

355 360 365

Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser

370 375 380

Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile

385 390 395 400

Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr

405 410 415

Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly

420 425 430

Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu

435 440 445

His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr

450 455 460

Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys

465 470

<210> 196

<211> 474

<212> PRT

<213> 人工的

<220>

<223> Lpd突变体

<400> 196

Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro

1 5 10 15

Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr

20 25 30

Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val

35 40 45

Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu

50 55 60

Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys

65 70 75 80

Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln

85 90 95

Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val

100 105 110

Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu

115 120 125

Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala

130 135 140

Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro

145 150 155 160

Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu

165 170 175

Arg Leu Leu Val Met Gly Gly Gly Ile Ile Ala Leu Glu Met Ala Thr

180 185 190

Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Val Arg Lys His

195 200 205

Gln Val Ile Arg Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys

210 215 220

Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala

225 230 235 240

Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys

245 250 255

Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly

260 265 270

Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu

275 280 285

Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn

290 295 300

Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu

305 310 315 320

Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala

325 330 335

Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr

340 345 350

Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys

355 360 365

Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser

370 375 380

Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile

385 390 395 400

Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr

405 410 415

Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly

420 425 430

Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu

435 440 445

His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr

450 455 460

Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys

465 470

<210> 197

<211> 1548

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 197

atgacggacc atacaatgaa gaaaaacccc gtaagtatac cacacaccgt ctggtacgcc 60

gacgatatcc gccgcggaga acgcgaggcg gcagatgtgc tggggctcac actctatgag 120

ctgatgcttc gcgctggcga ggccgcattc caggtgtgtc gttcggcgta tcctgacgcc 180

cgccactggc tggtgctgtg cggtcatggt aataacggcg gcgatggcta cgtggtcgcg 240

cgactggcca aagcggtcgg cattgaggtc acgttgttgg cccaggagag cgacaaaccg 300

ttgccggaag aggccgcgct ggcacgcgaa gcatggttaa acgcgggtgg cgagatccat 360

gcttcgaata ttgtctggcc cgaatcggta gatctgattg ttgatgcgct gctcggtacc 420

ggtttgcggc aagcgccccg cgaatccatt agccagttaa tcgaccacgc taattcccat 480

cctgcgccga ttgtggcggt tgatatccct tccggcctgc tggctgaaac tggcgctacg 540

ccaggcgcgg tgatcaacgc cgatcacacc atcactttta ttgcgctgaa accaggcttg 600

ctcactggaa aagcgcggga tgttaccgga caactgcatt ttgactcact ggggctggat 660

agttggctgg caggtcagga gacgaaaatt cagcggtttt cagcagaaca actttctcac 720

tggctaaaac cgcgtcgccc gacttcgcat aaaggcgatc acgggcggct ggtaattatc 780

ggtggcgatc acggcacggc gggggctatt cgtatgacgg gggaagcggc gctgcgtgct 840

ggtgctggtt tagtccgagt actgacccgc agtgaaaaca ttgcgccgct gctgactgca 900

cgaccggaat tgatggtgca tgaactgacg atggactctc ttaccgaaag cctggaatgg 960

gccgatgtgg tggtgattgg tcccggtctg ggccagcaag agtgggggaa aaaagcactg 1020

caaaaagttg agaattttcg caaaccgatg ttgtgggatg ccgatgcatt gaacctgctg 1080

gcaatcaatc ccgataagcg tcacaatcgc gtgatcacgc cgcatcctgg cgaggccgca 1140

cggttgttag gctgttccgt cgctgaaatt gaaagtgacc gcttacattg cgccaaacgt 1200

ctggtacaac gttatggcgg cgtagcggtg ctgaaaggtg ccggaaccgt ggtcgccgcc 1260

catcctgacg ctttaggcat tattgatgcc ggaaatgcag gcatggcgag cggcggcatg 1320

ggcgatgtgc tctctggtat tattggcgca ttgcttgggc aaaaactgtc gccgtatgat 1380

gcagcctgtg caggctgtgt cgcgcacggt gcggcagctg acgtactggc ggcgcgtttt 1440

ggaacgcgcg ggatgctggc aaccgatctc ttttccacgc tacagcgtat tgttaacccg 1500

gaagtgactg ataaaaacca tgatgaatcg agtaattccg ctccctga 1548

<210> 198

<211> 449

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 198

Met Ser Glu Ile Val Val Ser Lys Phe Gly Gly Thr Ser Val Ala Asp

1 5 10 15

Phe Asp Ala Met Asn Arg Ser Ala Asp Ile Val Leu Ser Asp Ala Asn

20 25 30

Val Arg Leu Val Val Leu Ser Ala Ser Ala Gly Ile Thr Asn Leu Leu

35 40 45

Val Ala Leu Ala Glu Gly Leu Glu Pro Gly Glu Arg Phe Glu Lys Leu

50 55 60

Asp Ala Ile Arg Asn Ile Gln Phe Ala Ile Leu Glu Arg Leu Arg Tyr

65 70 75 80

Pro Asn Val Ile Arg Glu Glu Ile Glu Arg Leu Leu Glu Asn Ile Thr

85 90 95

Val Leu Ala Glu Ala Ala Ala Leu Ala Thr Ser Pro Ala Leu Thr Asp

100 105 110

Glu Leu Val Ser His Gly Gly Leu Met Ser Thr Leu Leu Phe Val Glu

115 120 125

Ile Leu Arg Glu Arg Asp Val Gln Ala Gln Trp Phe Asp Val Arg Lys

130 135 140

Val Met Arg Thr Asn Asp Arg Phe Gly Arg Ala Glu Pro Asp Ile Ala

145 150 155 160

Ala Leu Ala Glu Leu Ala Ala Leu Gln Leu Leu Pro Arg Leu Asn Glu

165 170 175

Gly Leu Val Ile Thr Gln Gly Phe Ile Gly Ser Glu Asn Lys Gly Arg

180 185 190

Thr Thr Thr Leu Gly Arg Gly Gly Ser Asp Tyr Thr Ala Ala Leu Leu

195 200 205

Ala Glu Ala Leu His Ala Ser Arg Val Asp Ile Trp Thr Asp Val Pro

210 215 220

Gly Ile Tyr Thr Thr Asp Pro Arg Val Val Ser Ala Ala Lys Arg Ile

225 230 235 240

Asp Glu Ile Ala Phe Ala Glu Ala Ala Lys Met Ala Thr Phe Gly Ala

245 250 255

Lys Val Leu His Pro Ala Thr Leu Leu Pro Ala Val Arg Ser Asp Ile

260 265 270

Pro Val Phe Val Gly Ser Ser Lys Asp Pro Arg Ala Gly Gly Thr Leu

275 280 285

Val Cys Asn Lys Thr Glu Asn Pro Pro Leu Phe Arg Ala Leu Ala Leu

290 295 300

Arg Arg Asn Gln Thr Leu Leu Thr Leu His Ser Leu Asn Met Leu His

305 310 315 320

Ser Arg Gly Phe Leu Ala Glu Val Phe Gly Ile Leu Ala Arg His Asn

325 330 335

Ile Ser Val Asp Leu Ile Thr Thr Ser Glu Val Ser Val Ala Leu Thr

340 345 350

Leu Asp Thr Thr Gly Ser Thr Ser Thr Gly Asp Thr Leu Leu Thr Gln

355 360 365

Ser Leu Leu Met Glu Leu Ser Ala Leu Cys Arg Val Glu Val Glu Glu

370 375 380

Gly Leu Ala Leu Val Ala Leu Ile Gly Asn Asp Leu Ser Lys Ala Cys

385 390 395 400

Gly Val Gly Lys Glu Val Phe Gly Val Leu Glu Pro Phe Asn Ile Arg

405 410 415

Met Ile Cys Tyr Gly Ala Ser Ser His Asn Leu Cys Phe Leu Val Pro

420 425 430

Gly Glu Asp Ala Glu Gln Val Val Gln Lys Leu His Ser Asn Leu Phe

435 440 445

Glu

<210> 199

<211> 367

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 199

Met Lys Asn Val Gly Phe Ile Gly Trp Arg Gly Met Val Gly Ser Val

1 5 10 15

Leu Met Gln Arg Met Val Glu Glu Arg Asp Phe Asp Ala Ile Arg Pro

20 25 30

Val Phe Phe Ser Thr Ser Gln Leu Gly Gln Ala Ala Pro Ser Phe Gly

35 40 45

Gly Thr Thr Gly Thr Leu Gln Asp Ala Phe Asp Leu Glu Ala Leu Lys

50 55 60

Ala Leu Asp Ile Ile Val Thr Cys Gln Gly Gly Asp Tyr Thr Asn Glu

65 70 75 80

Ile Tyr Pro Lys Leu Arg Glu Ser Gly Trp Gln Gly Tyr Trp Ile Asp

85 90 95

Ala Ala Ser Ser Leu Arg Met Lys Asp Asp Ala Ile Ile Ile Leu Asp

100 105 110

Pro Val Asn Gln Asp Val Ile Thr Asp Gly Leu Asn Asn Gly Ile Arg

115 120 125

Thr Phe Val Gly Gly Asn Cys Thr Val Ser Leu Met Leu Met Ser Leu

130 135 140

Gly Gly Leu Phe Ala Asn Asp Leu Val Asp Trp Val Ser Val Ala Thr

145 150 155 160

Tyr Gln Ala Ala Ser Gly Gly Gly Ala Arg His Met Arg Glu Leu Leu

165 170 175

Thr Gln Met Gly His Leu Tyr Gly His Val Ala Asp Glu Leu Ala Thr

180 185 190

Pro Ser Ser Ala Ile Leu Asp Ile Glu Arg Lys Val Thr Thr Leu Thr

195 200 205

Arg Ser Gly Glu Leu Pro Val Asp Asn Phe Gly Val Pro Leu Ala Gly

210 215 220

Ser Leu Ile Pro Trp Ile Asp Lys Gln Leu Asp Asn Gly Gln Ser Arg

225 230 235 240

Gln Glu Trp Lys Gly Gln Ala Glu Thr Asn Lys Ile Leu Asn Thr Ser

245 250 255

Ser Val Ile Pro Val Asp Gly Leu Cys Val Arg Val Gly Ala Leu Arg

260 265 270

Cys His Ser Gln Ala Phe Thr Ile Lys Leu Lys Lys Asp Val Ser Ile

275 280 285

Pro Thr Val Glu Glu Leu Leu Ala Ala His Asn Pro Trp Ala Lys Val

290 295 300

Val Pro Asn Asp Arg Glu Ile Thr Met Arg Glu Leu Thr Pro Ala Ala

305 310 315 320

Val Thr Gly Thr Leu Thr Thr Pro Val Gly Arg Leu Arg Lys Leu Asn

325 330 335

Met Gly Pro Glu Phe Leu Ser Ala Phe Thr Val Gly Asp Gln Leu Leu

340 345 350

Trp Gly Ala Ala Glu Pro Leu Arg Arg Met Leu Arg Gln Leu Ala

355 360 365

<210> 200

<211> 360

<212> PRT

<213> 勤奋金属球菌(Metallosphaera sedula)

<400> 200

Met Lys Ala Ala Val Leu His Thr Tyr Lys Glu Pro Leu Ser Ile Glu

1 5 10 15

Asp Val Asn Ile Ser Gln Pro Lys Ala Gly Glu Val Lys Ile Lys Val

20 25 30

Lys Ala Thr Gly Leu Cys Arg Ser Asp Val His Val Phe Glu Gly Lys

35 40 45

Thr Pro Val Pro Pro Pro Val Val Ala Gly His Glu Ile Ser Gly Ile

50 55 60

Val Glu Glu Val Gly Pro Gly Val Thr Arg Val Lys Pro Gly Asp Arg

65 70 75 80

Val Ile Ser Ala Phe Ile His Pro Cys Gly Lys Cys Gly Asn Cys Val

85 90 95

Ala Gly Lys Glu Asn Leu Cys Glu Thr Phe Ser Gln Val Arg Leu Lys

100 105 110

Gly Val Met Pro Asp Gly Thr Ser Arg Leu Ser Lys Asp Gly Lys Glu

115 120 125

Ile Arg Thr Phe Leu Gly Gly Gly Phe Ala Glu Tyr Ala Ile Val Gly

130 135 140

Glu Asn Ala Leu Thr Arg Val Pro Glu Asp Met Asp Leu Glu Lys Val

145 150 155 160

Ala Val Leu Gly Cys Ala Gly Leu Thr Gly Tyr Gly Ala Ile Ser Ser

165 170 175

Ser Lys Ile Glu Pro Gly Asp Thr Val Ala Val Ile Gly Val Gly Gly

180 185 190

Val Gly Leu Ser Thr Ile Gln Leu Leu Arg Ala Ser Gly Ala Gly Arg

195 200 205

Ile Ile Ala Val Gly Thr Lys Lys Trp Lys Leu Asp Arg Ala Met Glu

210 215 220

Leu Gly Ala Thr Asp Val Val Asn Ser Lys Glu Ile Asp Pro Val Lys

225 230 235 240

Ala Ile Lys Glu Ile Thr Gly Gly Gly Pro Gln Val Val Ile Glu Ala

245 250 255

Gly Gly Asn Glu Asp Thr Ile His Met Ala Leu Asp Ser Val Arg Ile

260 265 270

Gly Gly Lys Val Val Leu Val Gly Leu Pro Pro Ala Thr Ala Met Ile

275 280 285

Pro Ile Arg Val Ala Ser Ile Val Arg Gly Gly Ile Glu Val Val Gly

290 295 300

Asn Tyr Gly Gly Arg Pro Arg Val Asp Met Pro Lys Leu Leu Glu Leu

305 310 315 320

Val Arg Gln Gly Arg Tyr Asp Pro Ser Arg Leu Val Thr Gly Arg Phe

325 330 335

Arg Leu Glu Glu Ile Asn Glu Ala Val Lys Met Leu Glu Glu Gly Glu

340 345 350

Ala Ile Arg Ser Leu Ile Ile Pro

355 360

<210> 201

<211> 359

<212> PRT

<213> 东工大硫化叶菌(Sulfolobus tokodaii)

<400> 201

Met Ile Leu Met Arg Arg Thr Leu Lys Ala Ala Ile Leu Gly Ala Thr

1 5 10 15

Gly Leu Val Gly Ile Glu Tyr Val Arg Met Leu Ser Asn His Pro Tyr

20 25 30

Ile Lys Pro Ala Tyr Leu Ala Gly Lys Gly Ser Val Gly Lys Pro Tyr

35 40 45

Gly Glu Val Val Arg Trp Gln Thr Val Gly Gln Val Pro Lys Glu Ile

50 55 60

Ala Asp Met Glu Ile Lys Pro Thr Asp Pro Lys Leu Met Asp Asp Val

65 70 75 80

Asp Ile Ile Phe Ser Pro Leu Pro Gln Gly Ala Ala Gly Pro Val Glu

85 90 95

Glu Gln Phe Ala Lys Glu Gly Phe Pro Val Ile Ser Asn Ser Pro Asp

100 105 110

His Arg Phe Asp Pro Asp Val Pro Leu Leu Val Pro Glu Leu Asn Pro

115 120 125

His Thr Ile Ser Leu Ile Asp Glu Gln Arg Lys Arg Arg Glu Trp Lys

130 135 140

Gly Phe Ile Val Thr Thr Pro Leu Cys Thr Ala Gln Gly Ala Ala Ile

145 150 155 160

Pro Leu Gly Ala Ile Phe Lys Asp Tyr Lys Met Asp Gly Ala Phe Ile

165 170 175

Thr Thr Ile Gln Ser Leu Ser Gly Ala Gly Tyr Pro Gly Ile Pro Ser

180 185 190

Leu Asp Val Val Asp Asn Ile Leu Pro Leu Gly Asp Gly Pro Asp Ala

195 200 205

Lys Thr Ile Lys Glu Ile Phe Arg Ile Leu Ser Glu Val Lys Arg Asn

210 215 220

Val Asp Glu Pro Lys Leu Glu Asp Val Ser Leu Ala Ala Thr Thr His

225 230 235 240

Arg Ile Ala Thr Ile His Gly His Tyr Glu Val Leu Tyr Val Ser Phe

245 250 255

Lys Glu Glu Thr Ala Ala Glu Lys Val Lys Glu Thr Leu Glu Asn Phe

260 265 270

Arg Gly Glu Pro Gln Asp Leu Lys Leu Pro Thr Ala Pro Ser Lys Pro

275 280 285

Ile Ile Val Met Asn Glu Asp Thr Arg Pro Gln Val Tyr Phe Asp Arg

290 295 300

Trp Ala Gly Asp Ile Pro Gly Met Ser Val Val Val Gly Arg Leu Lys

305 310 315 320

Gln Val Asn Lys Arg Met Ile Arg Leu Val Ser Leu Ile His Asn Thr

325 330 335

Val Arg Gly Ala Ala Gly Gly Gly Ile Leu Ala Ala Glu Leu Leu Val

340 345 350

Glu Lys Gly Tyr Ile Glu Lys

355

<210> 202

<211> 324

<212> PRT

<213> 扭脱甲基杆菌(Methylobacter extorquens)

<400> 202

Met Ser Phe Thr Leu Ile Gln Gln Ala Thr Pro Arg Leu His Arg Ser

1 5 10 15

Glu Leu Ala Val Pro Gly Ser Asn Pro Thr Phe Met Glu Lys Ser Ala

20 25 30

Ala Ser Lys Ala Asp Val Ile Phe Leu Asp Leu Glu Asp Ala Val Ala

35 40 45

Pro Asp Asp Lys Glu Gln Ala Arg Lys Asn Ile Ile Gln Ala Leu Asn

50 55 60

Asp Leu Asp Trp Gly Asn Lys Thr Met Met Ile Arg Ile Asn Gly Leu

65 70 75 80

Asp Thr His Tyr Met Tyr Arg Asp Val Val Asp Ile Val Glu Ala Cys

85 90 95

Pro Arg Leu Asp Met Ile Leu Ile Pro Lys Val Gly Val Pro Ala Asp

100 105 110

Val Tyr Ala Ile Asp Val Leu Thr Thr Gln Ile Glu Gln Ala Lys Lys

115 120 125

Arg Glu Lys Lys Ile Gly Phe Glu Val Leu Ile Glu Thr Ala Leu Gly

130 135 140

Met Ala Asn Val Glu Ala Ile Ala Thr Ser Ser Lys Arg Leu Glu Ala

145 150 155 160

Met Ser Phe Gly Val Ala Asp Tyr Ala Ala Ser Thr Arg Ala Arg Ser

165 170 175

Thr Val Ile Gly Gly Val Asn Ala Asp Tyr Ser Val Leu Thr Asp Lys

180 185 190

Asp Glu Ala Gly Asn Arg Gln Thr His Trp Gln Asp Pro Trp Leu Phe

195 200 205

Ala Gln Asn Arg Met Leu Val Ala Cys Arg Ala Tyr Gly Leu Arg Pro

210 215 220

Ile Asp Gly Pro Phe Gly Asp Phe Ser Asp Pro Asp Gly Tyr Thr Ser

225 230 235 240

Ala Ala Arg Arg Cys Ala Ala Leu Gly Phe Glu Gly Lys Trp Ala Ile

245 250 255

His Pro Ser Gln Ile Asp Leu Ala Asn Glu Val Phe Thr Pro Ser Glu

260 265 270

Ala Glu Val Thr Lys Ala Arg Arg Ile Leu Glu Ala Met Glu Glu Ala

275 280 285

Ala Lys Ala Gly Arg Gly Ala Val Ser Leu Asp Gly Arg Leu Ile Asp

290 295 300

Ile Ala Ser Ile Arg Met Ala Glu Ala Leu Ile Gln Lys Ala Asp Ala

305 310 315 320

Met Gly Gly Lys

<210> 203

<211> 309

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 203

Met Thr Thr Lys Lys Ala Asp Tyr Ile Trp Phe Asn Gly Glu Met Val

1 5 10 15

Arg Trp Glu Asp Ala Lys Val His Val Met Ser His Ala Leu His Tyr

20 25 30

Gly Thr Ser Val Phe Glu Gly Ile Arg Cys Tyr Asp Ser His Lys Gly

35 40 45

Pro Val Val Phe Arg His Arg Glu His Met Gln Arg Leu His Asp Ser

50 55 60

Ala Lys Ile Tyr Arg Phe Pro Val Ser Gln Ser Ile Asp Glu Leu Met

65 70 75 80

Glu Ala Cys Arg Asp Val Ile Arg Lys Asn Asn Leu Thr Ser Ala Tyr

85 90 95

Ile Arg Pro Leu Ile Phe Val Gly Asp Val Gly Met Gly Val Asn Pro

100 105 110

Pro Ala Gly Tyr Ser Thr Asp Val Ile Ile Ala Ala Phe Pro Trp Gly

115 120 125

Ala Tyr Leu Gly Ala Glu Ala Leu Glu Gln Gly Ile Asp Ala Met Val

130 135 140

Ser Ser Trp Asn Arg Ala Ala Pro Asn Thr Ile Pro Thr Ala Ala Lys

145 150 155 160

Ala Gly Gly Asn Tyr Leu Ser Ser Leu Leu Val Gly Ser Glu Ala Arg

165 170 175

Arg His Gly Tyr Gln Glu Gly Ile Ala Leu Asp Val Asn Gly Tyr Ile

180 185 190

Ser Glu Gly Ala Gly Glu Asn Leu Phe Glu Val Lys Asp Gly Val Leu

195 200 205

Phe Thr Pro Pro Phe Thr Ser Ser Ala Leu Pro Gly Ile Thr Arg Asp

210 215 220

Ala Ile Ile Lys Leu Ala Lys Glu Leu Gly Ile Glu Val Arg Glu Gln

225 230 235 240

Val Leu Ser Arg Glu Ser Leu Tyr Leu Ala Asp Glu Val Phe Met Ser

245 250 255

Gly Thr Ala Ala Glu Ile Thr Pro Val Arg Ser Val Asp Gly Ile Gln

260 265 270

Val Gly Glu Gly Arg Cys Gly Pro Val Thr Lys Arg Ile Gln Gln Ala

275 280 285

Phe Phe Gly Leu Phe Thr Gly Glu Thr Glu Asp Lys Trp Gly Trp Leu

290 295 300

Asp Gln Val Asn Gln

305

<210> 204

<211> 329

<212> PRT

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 204

Met Lys Leu Ala Val Tyr Ser Thr Lys Gln Tyr Asp Lys Lys Tyr Leu

1 5 10 15

Gln Gln Val Asn Glu Ser Phe Gly Phe Glu Leu Glu Phe Phe Asp Phe

20 25 30

Leu Leu Thr Glu Lys Thr Ala Lys Thr Ala Asn Gly Cys Glu Ala Val

35 40 45

Cys Ile Phe Val Asn Asp Asp Gly Ser Arg Pro Val Leu Glu Glu Leu

50 55 60

Lys Lys His Gly Val Lys Tyr Ile Ala Leu Arg Cys Ala Gly Phe Asn

65 70 75 80

Asn Val Asp Leu Asp Ala Ala Lys Glu Leu Gly Leu Lys Val Val Arg

85 90 95

Val Pro Ala Tyr Asp Pro Glu Ala Val Ala Glu His Ala Ile Gly Met

100 105 110

Met Met Thr Leu Asn Arg Arg Ile His Arg Ala Tyr Gln Arg Thr Arg

115 120 125

Asp Ala Asn Phe Ser Leu Glu Gly Leu Thr Gly Phe Thr Met Tyr Gly

130 135 140

Lys Thr Ala Gly Val Ile Gly Thr Gly Lys Ile Gly Val Ala Met Leu

145 150 155 160

Arg Ile Leu Lys Gly Phe Gly Met Arg Leu Leu Ala Phe Asp Pro Tyr

165 170 175

Pro Ser Ala Ala Ala Leu Glu Leu Gly Val Glu Tyr Val Asp Leu Pro

180 185 190

Thr Leu Phe Ser Glu Ser Asp Val Ile Ser Leu His Cys Pro Leu Thr

195 200 205

Pro Glu Asn Tyr His Leu Leu Asn Glu Ala Ala Phe Glu Gln Met Lys

210 215 220

Asn Gly Val Met Ile Val Asn Thr Ser Arg Gly Ala Leu Ile Asp Ser

225 230 235 240

Gln Ala Ala Ile Glu Ala Leu Lys Asn Gln Lys Ile Gly Ser Leu Gly

245 250 255

Met Asp Val Tyr Glu Asn Glu Arg Asp Leu Phe Phe Glu Asp Lys Ser

260 265 270

Asn Asp Val Ile Gln Asp Asp Val Phe Arg Arg Leu Ser Ala Cys His

275 280 285

Asn Val Leu Phe Thr Gly His Gln Ala Phe Leu Thr Ala Glu Ala Leu

290 295 300

Thr Ser Ile Ser Gln Thr Thr Leu Gln Asn Leu Ser Asn Leu Glu Lys

305 310 315 320

Gly Glu Thr Cys Pro Asn Glu Leu Val

325

<210> 205

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失xylAB 1

<400> 205

acgacatcat ccatcacccg cggcattacc tgattatgga gttcaatatg 50

<210> 206

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失xylAB 2

<400> 206

cccccacccg gtcaggcagg ggataacgtt tacgccatta atggcagaag 50

<210> 207

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失yjhH 1

<400> 207

aatgcgcgaa gttgccgact tcctgattaa taaaggggtc gacgggctgt 50

<210> 208

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失yjhH 2

<400> 208

gtaccgactt aactgtgttg atcatcgtac gcaagtgacc aacgctgtcg 50

<210> 209

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失yagE 1

<400> 209

ggcggcacca acgcccggga aaccatcgaa ctcagccagc acgcgcagca 50

<210> 210

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失yagE 2

<400> 210

agcacggtga agtgcggatg ggcacctttg acggtatgga tcatgctgcg 50

<210> 211

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失yiaE 1

<400> 211

catatttcag gctaaggtga tcgccttatc agtgaatgga gagaagcatg 50

<210> 212

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失yiaE 2

<400> 212

tatcgggctt tactctacgc agtcgcggct tagtccgcga cgtgcggatt 50

<210> 213

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失ycdW 1

<400> 213

aacgataagt gcgaataaat ttcgcacaac gcttttcggg agtcagtatg 50

<210> 214

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失ycdW 2

<400> 214

ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc 50

<210> 215

<211> 100

<212> DNA

<213> 人工的

<220>

<223> 引物缺失ptsG 1

<400> 215

atgtttaaga atgcatttgc taacctgcaa aaggtcggta aatcgctgat gctgccggta 60

tccgtactgc ctatcgcagg tgtaggctgg agctgcttcg 100

<210> 216

<211> 100

<212> DNA

<213> 人工的

<220>

<223> 引物缺失ptsG 2

<400> 216

ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa 60

aatcgcctga acaccagaac catatgaata tcctccttag 100

<210> 217

<211> 21

<212> DNA

<213> 人工的

<220>

<223> RBS120

<400> 217

atccggtata ggaggtatag a 21

<210> 218

<211> 100

<212> DNA

<213> 人工的

<220>

<223> 引物缺失udhA 1

<400> 218

ggtgcgcgcg tcgcagttat cgagcgttat caaaatgttg gcggcggttg cacccactgg 60

ggcaccatcc cgtcgaaagc catatgaata tcctccttag 100

<210> 219

<211> 100

<212> DNA

<213> 人工的

<220>

<223> 引物缺失udhA 2

<400> 219

cccagaatct cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg 60

acgatttgtg cgcgtgccag tgtaggctgg agctgcttcg 100

<210> 220

<211> 101

<212> DNA

<213> 人工的

<220>

<223> 引物缺失pfkA 1

<400> 220

gttcctcggt tctgcgcgtt tcccggaatt ccgcgacgag aacatccgcg ccgtggctat 60

cgaaaacctg aaaaaacgtg gtgtaggctg gagctgcttc g 101

<210> 221

<211> 102

<212> DNA

<213> 人工的

<220>

<223> 引物缺失pfkA 2

<400> 221

ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc agggtaaagg 60

aatctgcctt tttccgaaat cacatatgaa tatcctcctt ag 102

<210> 222

<211> 2463

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 222

atgagagtgt tgaagttcgg cggtacatca gtggcaaatg cagaacgttt tctgcgtgtt 60

gccgatattc tggaaagcaa tgccaggcag gggcaggtgg ccaccgtcct ctctgccccc 120

gccaaaatca ccaaccacct ggtggcgatg attgaaaaaa ccattagcgg ccaggatgct 180

ttacccaata tcagcgatgc cgaacgtatt tttgccgaac ttttgacggg actcgccgcc 240

gcccagccgg ggttcccgct ggcgcaattg aaaactttcg tcgatcagga atttgcccaa 300

ataaaacatg tcctgcatgg cattagtttg ttggggcagt gcccggatag catcaacgct 360

gcgctgattt gccgtggcga gaaaatgtcg atcgccatta tggccggcgt attagaagcg 420

cgcggtcaca acgttactgt tatcgatccg gtcgaaaaac tgctggcagt ggggcattac 480

ctcgaatcta ccgtcgatat tgctgagtcc acccgccgta ttgcggcaag ccgcattccg 540

gctgatcaca tggtgctgat ggcaggtttc accgccggta atgaaaaagg cgaactggtg 600

gtgcttggac gcaacggttc cgactactct gctgcggtgc tggctgcctg tttacgcgcc 660

gattgttgcg agatttggac ggacgttgac ggggtctata cctgcgaccc gcgtcaggtg 720

cccgatgcga ggttgttgaa gtcgatgtcc taccaggaag cgatggagct ttcctacttc 780

ggcgctaaag ttcttcaccc ccgcaccatt acccccatcg cccagttcca gatcccttgc 840

ctgattaaaa ataccggaaa tcctcaagca ccaggtacgc tcattggtgc cagccgtgat 900

gaagacgaat taccggtcaa gggcatttcc aatctgaata acatggcaat gtccagcgtt 960

tctggtccgg ggatgaaagg gatggtcggc atggcggcgc gcgtctttgc agcgatgtca 1020

cgcgcccgta tttccgtggt gctgattacg caatcatctt ccgaatacag catcagtttc 1080

tgcgttccac aaagcgactg tgtgcgagct gaacgggcaa tgcaggaaga gttctacctg 1140

gaactgaaag aaggcttact ggagccgctg gcagtgacgg aacggctggc cattatctcg 1200

gtggtaggtg atggtatgcg caccttgcgt gggatctcgg cgaaattctt tgccgcactg 1260

gcccgcgcca atatcaacat tgtcgccatt gctcagggat cttctgaacg ctcaatctct 1320

gtcgtggtaa ataacgatga tgcgaccact ggcgtgcgcg ttactcatca gatgctgttc 1380

aataccgatc aggttatcga agtgtttgtg attggcgtcg gtggcgttgg cggtgcgctg 1440

ctggagcaac tgaagcgtca gcaaagctgg ctgaagaata aacatatcga cttacgtgtc 1500

tgcggtgttg ccaactcgaa ggctctgctc accaatgtac atggccttaa tctggaaaac 1560

tggcaggaag aactggcgca agccaaagag ccgtttaatc tcgggcgctt aattcgcctc 1620

gtgaaagaat atcatctgct gaacccggtc attgttgact gcacttccag ccaggcagtg 1680

gcggatcaat atgccgactt cctgcgcgaa ggtttccacg ttgtcacgcc gaacaaaaag 1740

gccaacacct cgtcgatgga ttactaccat cagttgcgtt atgcggcgga aaaatcgcgg 1800

cgtaaattcc tctatgacac caacgttggg gctggattac cggttattga gaacctgcaa 1860

aatctgctca atgcaggtga tgaattgatg aagttctccg gcattctttc tggttcgctt 1920

tcttatatct tcggcaagtt agacgaaggc atgagtttct ccgaggcgac cacgctggcg 1980

cgggaaatgg gttataccga accggacccg cgagatgatc tttctggtat ggatgtggcg 2040

cgtaaactat tgattctcgc tcgtgaaacg ggacgtgaac tggagctggc ggatattgaa 2100

attgaacctg tgctgcccgc agagtttaac gccgagggtg atgttgccgc ttttatggcg 2160

aatctgtcac aactcgacga tctctttgcc gcgcgcgtgg cgaaggcccg tgatgaagga 2220

aaagttttgc gctatgttgg caatattgat gaagatggcg tctgccgcgt gaagattgcc 2280

gaagtggatg gtaatgatcc gctgttcaaa gtgaaaaatg gcgaaaacgc cctggccttc 2340

tatagccact attatcagcc gctgccgttg gtactgcgcg gatatggtgc gggcaatgac 2400

gttacagctg ccggtgtctt tgctgatctg ctacgtaccc tctcatggaa gttaggagtc 2460

tga 2463

<210> 223

<211> 3465

<212> DNA

<213> 菜豆根瘤菌(Rhizobium etli)

<400> 223

atgcccatat ccaagatact cgttgccaat cgctctgaaa tagccatccg cgtgttccgc 60

gcggccaacg agcttggaat aaaaacggtg gcgatctggg cggaagagga caagctggcg 120

ctgcaccgct tcaaggcgga cgagagttat caggtcggcc gcggaccgca tcttgcccgc 180

gacctcgggc cgatcgaaag ctatctgtcg atcgacgagg tgatccgcgt cgccaagctt 240

tccggtgccg acgccatcca tccgggctac ggcctcttgt cggaaagccc cgaattcgtc 300

gatgcctgca acaaggccgg catcatcttc atcggcccga aggccgatac gatgcgccag 360

cttggcaaca aggtcgcagc gcgcaacctg gcgatctcgg tcggcgtacc ggtcgtgccg 420

gcgaccgagc cactgccgga cgatatggcc gaagtggcga agatggcggc ggcgatcggc 480

tatcccgtca tgctgaaggc atcctggggc ggcggcggtc gcggcatgcg cgtcattcgt 540

tccgaggccg acctcgccaa ggaagtgacg gaagccaagc gcgaggcgat ggcggccttc 600

ggcaaggacg aggtctatct cgaaaaactg gtcgagcgcg cccgccacgt cgaaagccag 660

atcctcggcg acacccacgg caatgtcgtg catctcttcg agcgcgactg ttccgttcag 720

cgccgcaatc agaaggtcgt cgagcgcgcg cccgcaccct atctttcgga agcgcagcgc 780

caggaactcg ccgcctattc gctgaagatc gcaggggcga ccaactatat cggcgccggc 840

accgtcgaat atctgatgga tgccgatacc ggcaaatttt acttcatcga agtcaatccg 900

cgcatccagg tcgagcacac ggtgaccgaa gtcgtcaccg gcatcgatat cgtcaaggcg 960

cagatccaca tcctggacgg cgccgcgatc ggcacgccgc aatccggcgt gccgaaccag 1020

gaagacatcc gtctcaacgg tcacgccctg cagtgccgcg tgacgacgga agatccggag 1080

cacaacttca ttccggatta cggccgcatc accgcctatc gctcggcttc cggcttcggc 1140

atccggcttg acggcggcac ctcttattcc ggcgccatca tcacccgcta ttacgatccg 1200

ctgctcgtca aggtcacggc ctgggcgccg aacccgctgg aagccatttc ccgcatggac 1260

cgggcgctgc gcgaattccg catccgtggc gtcgccacca acctgacctt cctcgaagcg 1320

atcatcggcc atccgaaatt ccgcgacaac agctacacca cccgcttcat cgacacgacg 1380

ccggagctct tccagcaggt caagcgccag gaccgcgcga cgaagcttct gacctatctc 1440

gccgacgtca ccgtcaatgg ccatcccgag gccaaggaca ggccgaagcc cctcgagaat 1500

gccgccaggc cggtggtgcc ctatgccaat ggcaacgggg tgaaggacgg caccaagcag 1560

ctgctcgata cgctcggccc gaaaaaattc ggcgaatgga tgcgcaatga gaagcgcgtg 1620

cttctgaccg acaccacgat gcgcgacggc caccagtcgc tgctcgcaac ccgcatgcgt 1680

acctatgaca tcgccaggat cgccggcacc tattcgcatg cgctgccgaa cctcttgtcg 1740

ctcgaatgct ggggcggcgc caccttcgac gtctcgatgc gcttcctcac cgaagatccg 1800

tgggagcggc tggcgctgat ccgagagggg gcgccgaacc tgctcctgca gatgctgctg 1860

cgcggcgcca atggcgtcgg ttacaccaac tatcccgaca atgtcgtcaa atacttcgtc 1920

cgccaggcgg ccaaaggcgg catcgatctc ttccgcgtct tcgactgcct gaactgggtc 1980

gagaatatgc gggtgtcgat ggatgcgatt gccgaggaga acaagctctg cgaggcggcg 2040

atctgctaca ccggcgatat cctcaattcc gcccgcccga aatacgactt gaaatattac 2100

accaaccttg ccgtcgagct tgagaaggcc ggcgcccata tcattgcggt caaggatatg 2160

gcgggccttc tgaagccggc tgctgccaag gttctgttca aggcgctgcg tgaagcaacc 2220

ggcctgccga tccatttcca cacgcatgac acctcgggca ttgcggcggc aacggttctt 2280

gccgccgtcg aagccggtgt cgatgccgtc gatgcggcga tggatgcgct ctccggcaac 2340

acctcgcaac cctgtctcgg ctcgatcgtc gaggcgctct ccggctccga gcgcgatccc 2400

ggcctcgatc cggcatggat ccgccgcatc tccttctatt gggaagcggt gcgcaaccag 2460

tatgccgcct tcgaaagcga cctcaaggga ccggcatcgg aagtctatct gcatgaaatg 2520

ccgggcggcc agttcaccaa cctcaaggag caggcccgct cgctggggct ggaaacccgc 2580

tggcaccagg tggcgcaggc ctatgccgac gccaaccaga tgttcggcga tatcgtcaag 2640

gtgacgccat cctccaaggt cgtcggcgac atggcgctga tgatggtctc ccaggacctg 2700

accgtcgccg atgtcgtcag ccccgaccgc gaagtctcct tcccggaatc ggtcgtctcg 2760

atgctgaagg gcgatctcgg ccagcctccg tctggatggc cggaagcgct gcagaagaaa 2820

gcattgaagg gcgaaaagcc ctatacggtg cgccccggct cgctgctcaa ggaagccgat 2880

ctcgatgcgg aacgcaaagt catcgagaag aagcttgagc gcgaggtcag cgacttcgaa 2940

ttcgcttcct atctgatgta tccgaaggtc ttcaccgact ttgcgcttgc ctccgatacc 3000

tacggtccgg tttcggtgct gccgacgccc gcctattttt acgggttggc ggacggcgag 3060

gagctgttcg ccgacatcga gaagggcaag acgctcgtca tcgtcaatca ggcggtgagc 3120

gccaccgaca gccagggcat ggtcactgtc ttcttcgagc tcaacggcca gccgcgccgt 3180

atcaaggtgc ccgatcgggc ccacggggcg acgggagccg ccgtgcgccg caaggccgaa 3240

cccggcaatg ccgcccatgt cggtgcgccg atgccgggcg tcatcagccg tgtctttgtc 3300

tcttcaggcc aggccgtcaa tgccggcgac gtgctcgtct ccatcgaggc catgaagatg 3360

gaaaccgcga tccatgcgga aaaggacggc accattgccg aagtgctggt caaggccggc 3420

gatcagatcg atgccaagga cctgctggcg gtttacggcg gatga 3465

<210> 224

<211> 62

<212> DNA

<213> 人工的

<220>

<223> 启动子Ptrc

<400> 224

gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag cggataacaa 60

tt 62

<210> 225

<211> 121

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 225

caagcccaaa ggaagagtga ggcgagtcag tcgcgtaatg cttaggcaca ggattgattt 60

gtcgcaatga ttgacacgat tccgcttgac gctgcgtaag gtttttgtaa ttttacaggc 120

a 121

<210> 226

<211> 933

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 226

atggttaaag tttatgcccc ggcttccagt gccaatatga gcgtcgggtt tgatgtgctc 60

ggggcggcgg tgacacctgt tgatggtgca ttgctcggag atgtagtcac ggttgaggcg 120

gcagagacat tcagtctcaa caacctcgga cgctttgccg ataagctgcc gtcagaacca 180

cgggaaaata tcgtttatca gtgctgggag cgtttttgcc aggaactggg taagcaaatt 240

ccagtggcga tgaccctgga aaagaatatg ccgatcggtt cgggcttagg ctccagtgcc 300

tgttcggtgg tcgcggcgct gatggcgatg aatgaacact gcggcaagcc gcttaatgac 360

actcgtttgc tggctttgat gggcgagctg gaaggccgta tctccggcag cattcattac 420

gacaacgtgg caccgtgttt tctcggtggt atgcagttga tgatcgaaga aaacgacatc 480

atcagccagc aagtgccagg gtttgatgag tggctgtggg tgctggcgta tccggggatt 540

aaagtctcga cggcagaagc cagggctatt ttaccggcgc agtatcgccg ccaggattgc 600

attgcgcacg ggcgacatct ggcaggcttc attcacgcct gctattcccg tcagcctgag 660

cttgccgcga agctgatgaa agatgttatc gctgaaccct accgtgaacg gttactgcca 720

ggcttccggc aggcgcggca ggcggtcgcg gaaatcggcg cggtagcgag cggtatctcc 780

ggctccggcc cgaccttgtt cgctctgtgt gacaagccgg aaaccgccca gcgcgttgcc 840

gactggttgg gtaagaacta cctgcaaaat caggaaggtt ttgttcatat ttgccggctg 900

gatacggcgg gcgcacgagt actggaaaac taa 933

<210> 227

<211> 1287

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 227

atgaaactct acaatctgaa agatcacaac gagcaggtca gctttgcgca agccgtaacc 60

caggggttgg gcaaaaatca ggggctgttt tttccgcacg acctgccgga attcagcctg 120

actgaaattg atgagatgct gaagctggat tttgtcaccc gcagtgcgaa gatcctctcg 180

gcgtttattg gtgatgaaat cccacaggaa atcctggaag agcgcgtgcg cgcggcgttt 240

gccttcccgg ctccggtcgc caatgttgaa agcgatgtcg gttgtctgga attgttccac 300

gggccaacgc tggcatttaa agatttcggc ggtcgcttta tggcacaaat gctgacccat 360

attgcgggtg ataagccagt gaccattctg accgcgacct ccggtgatac cggagcggca 420

gtggctcatg ctttctacgg tttaccgaat gtgaaagtgg ttatcctcta tccacgaggc 480

aaaatcagtc cactgcaaga aaaactgttc tgtacattgg gcggcaatat cgaaactgtt 540

gccatcgacg gcgatttcga tgcctgtcag gcgctggtga agcaggcgtt tgatgatgaa 600

gaactgaaag tggcgctagg gttaaactcg gctaactcga ttaacatcag ccgtttgctg 660

gcgcagattt gctactactt tgaagctgtt gcgcagctgc cgcaggagac gcgcaaccag 720

ctggttgtct cggtgccaag cggaaacttc ggcgatttga cggcgggtct gctggcgaag 780

tcactcggtc tgccggtgaa acgttttatt gctgcgacca acgtgaacga taccgtgcca 840

cgtttcctgc acgacggtca gtggtcaccc aaagcgactc aggcgacgtt atccaacgcg 900

atggacgtga gtcagccgaa caactggccg cgtgtggaag agttgttccg ccgcaaaatc 960

tggcaactga aagagctggg ttatgcagcc gtggatgatg aaaccacgca acagacaatg 1020

cgtgagttaa aagaactggg ctacacttcg gagccgcacg ctgccgtagc ttatcgtgcg 1080

ctgcgtgatc agttgaatcc aggcgaatat ggcttgttcc tcggcaccgc gcatccggcg 1140

aaatttaaag agagcgtgga agcgattctc ggtgaaacgt tggatctgcc aaaagagctg 1200

gcagaacgtg ctgatttacc cttgctttca cataatctgc ccgccgattt tgctgcgttg 1260

cgtaaattga tgatgaatca tcagtaa 1287

<210> 228

<211> 930

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 228

atgccgattc gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc 60

tttgtgatga caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc 120

cttaacctga tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac 180

tcacctttgc aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg 240

cccgcagagc atctgaacaa cttctactgt aactttgaag atattcagga tcagaacttt 300

gacggtttga ttgtaactgg tgcgccgctg ggcctggtgg agtttaatga tgtcgcttac 360

tggccgcaga tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt 420

gtctgctggg cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc 480

accgaaaaac tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg 540

cgtggctttg atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg 600

ttgattcgtg attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat 660

ctgtttgcca gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg 720

caaacgctgg cgcaggaatt tttccgcgat gtggaagccg gactagaccc ggatgtaccg 780

tataactatt tcccgcacaa tgatccgcaa aatacaccgc gagcgagctg gcgtagtcac 840

ggtaatttac tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat 900

ctacggcaca tgaatccaac gctggattaa 930

<210> 229

<211> 34

<212> DNA

<213> T7噬菌体

<400> 229

tcacactggc tcaccttcgg gtgggccttt ctgc 34

<210> 230

<211> 66

<212> DNA

<213> 人工的

<220>

<223> 用于弱化thrBC和metA表达的启动子

<400> 230

gagctgttgg cgattaatca tccggctcgt atattgtgtg gggttgcatg tactggagga 60

cagacc 66

<210> 231

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物弱化thrBC 1

<400> 231

atggttaaag tttatgcccc ggcttccagt gccaatatga gcgtcgggtt 50

<210> 232

<211> 66

<212> DNA

<213> 人工的

<220>

<223> 引物弱化thrBC 2

<400> 232

gacgttacag ctgccggtgt ctttgctgat ctgctacgta ccctctcatg gaagttagga 60

gtctga 66

<210> 233

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物弱化metA 1

<400> 233

atgccgattc gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga 50

<210> 234

<211> 66

<212> DNA

<213> 人工的

<220>

<223> 引物弱化metA 2

<400> 234

tttctggtta tcttcagcta tctggatgtc taaacgtata agcgtatgta gtgaggtaat 60

caggtt 66

<210> 235

<211> 54

<212> DNA

<213> 人工的

<220>

<223> 启动子Ptac

<400> 235

tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac aatt 54

<210> 236

<211> 17

<212> DNA

<213> T7噬菌体

<400> 236

ttaactttaa gaaggag 17

<210> 237

<211> 329

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 237

Met Lys Leu Ala Val Tyr Ser Thr Lys Gln Tyr Asp Lys Lys Tyr Leu

1 5 10 15

Gln Gln Val Asn Glu Ser Phe Gly Phe Glu Leu Glu Phe Phe Asp Phe

20 25 30

Leu Leu Thr Glu Lys Thr Ala Lys Thr Ala Asn Gly Cys Glu Ala Val

35 40 45

Cys Ile Phe Val Asn Asp Asp Gly Ser Arg Pro Val Leu Glu Glu Leu

50 55 60

Lys Lys His Gly Val Lys Tyr Ile Ala Leu Arg Cys Ala Gly Phe Asn

65 70 75 80

Asn Val Asp Leu Asp Ala Ala Lys Glu Leu Gly Leu Lys Val Val Arg

85 90 95

Val Pro Ala Tyr Asp Pro Glu Ala Val Ala Glu His Ala Ile Gly Met

100 105 110

Met Met Thr Leu Asn Arg Arg Ile His Arg Ala Tyr Gln Arg Thr Arg

115 120 125

Asp Ala Asn Phe Ser Leu Glu Gly Leu Thr Gly Phe Thr Met Tyr Gly

130 135 140

Lys Thr Ala Gly Val Ile Gly Thr Gly Lys Ile Gly Val Ala Met Leu

145 150 155 160

Arg Ile Leu Lys Gly Phe Gly Met Arg Leu Leu Ala Phe Asp Pro Tyr

165 170 175

Pro Ser Ala Ala Ala Leu Glu Leu Gly Val Glu Tyr Val Asp Leu Pro

180 185 190

Thr Leu Phe Ser Glu Ser Asp Val Ile Ser Leu His Cys Pro Leu Thr

195 200 205

Pro Glu Asn Tyr His Leu Leu Asn Glu Ala Ala Phe Glu Gln Met Lys

210 215 220

Asn Gly Val Met Ile Val Asn Thr Ser Arg Gly Ala Leu Ile Asp Ser

225 230 235 240

Gln Ala Ala Ile Glu Ala Leu Lys Asn Gln Lys Ile Gly Ser Leu Gly

245 250 255

Met Asp Val Tyr Glu Asn Glu Arg Asp Leu Phe Phe Glu Asp Lys Ser

260 265 270

Asn Asp Val Ile Gln Asp Asp Val Phe Arg Arg Leu Ser Ala Cys His

275 280 285

Asn Val Leu Phe Thr Gly His Gln Ala Phe Leu Thr Ala Glu Ala Leu

290 295 300

Thr Ser Ile Ser Gln Thr Thr Leu Gln Asn Leu Ser Asn Leu Glu Lys

305 310 315 320

Gly Glu Thr Cys Pro Asn Glu Leu Val

325

<210> 238

<211> 2676

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 238

atggctgtta ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag 60

cgtgaatatg ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc cgccgctctg 120

gctgctgcag atgctcgaat cccactcgcg aaaatggccg ttgccgaatc cggcatgggt 180

atcgtcgaag ataaagtgat caaaaaccac tttgcttctg aatatatcta caacgcctat 240

aaagatgaaa aaacctgtgg tgttctgtct gaagacgaca cttttggtac catcactatc 300

gctgaaccaa tcggtattat ttgcggtatc gttccgacca ctaacccgac ttcaactgct 360

atcttcaaat cgctgatcag tctgaagacc cgtaacgcca ttatcttctc cccgcacccg 420

cgtgcaaaag atgccaccaa caaagcggct gatatcgttc tgcaggctgc tatcgctgcc 480

ggtgctccga aagatctgat cggctggatc gatcaacctt ctgttgaact gtctaacgca 540

ctgatgcacc acccagacat caacctgatc ctcgcgactg gtggtccggg catggttaaa 600

gccgcataca gctccggtaa accagctatc ggtgtaggcg cgggcaacac tccagttgtt 660

atcgatgaaa ctgctgatat caaacgtgca gttgcatctg tactgatgtc caaaaccttc 720

gacaacggcg taatctgtgc ttctgaacag tctgttgttg ttgttgactc tgtttatgac 780

gctgtacgtg aacgttttgc aacccacggc ggctatctgt tgcagggtaa agagctgaaa 840

gctgttcagg atgttatcct gaaaaacggt gcgctgaacg cggctatcgt tggtcagcca 900

gcctataaaa ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa gattctgatc 960

ggtgaagtga ccgttgttga tgaaagcgaa ccgttcgcac atgaaaaact gtccccgact 1020

ctggcaatgt accgcgctaa agatttcgaa gacgcggtag aaaaagcaga gaaactggtt 1080

gctatgggcg gtatcggtca tacctcttgc ctgtacactg accaggataa ccaaccggct 1140

cgcgtttctt acttcggtca gaaaatgaaa acggcgcgta tcctgattaa caccccagcg 1200

tctcagggtg gtatcggtga cctgtataac ttcaaactcg caccttccct gactctgggt 1260

tgtggttctt ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca cctgatcaac 1320

aagaaaaccg ttgctaagcg agctgaaaac atgttgtggc acaaacttcc gaaatctatc 1380

tacttccgcc gtggctccct gccaatcgcg ctggatgaag tgattactga tggccacaaa 1440

cgtgcgctca tcgtgactga ccgcttcctg ttcaacaatg gttatgctga tcagatcact 1500

tccgtactga aagcagcagg cgttgaaact gaagtcttct tcgaagtaga agcggacccg 1560

accctgagca tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc agacgtgatt 1620

atcgcgctgg gtggtggttc cccgatggac gccgcgaaga tcatgtgggt tatgtacgaa 1680

catccggaaa ctcacttcga agagctggcg ctgcgcttta tggatatccg taaacgtatc 1740

tacaagttcc cgaaaatggg cgtgaaagcg aaaatgatcg ctgtcaccac cacttctggt 1800

acaggttctg aagtcactcc gtttgcggtt gtaactgacg acgctactgg tcagaaatat 1860

ccgctggcag actatgcgct gactccggat atggcgattg tcgacgccaa cctggttatg 1920

gacatgccga agtccctgtg tgctttcggt ggtctggacg cagtaactca cgccatggaa 1980

gcttatgttt ctgtactggc atctgagttc tctgatggtc aggctctgca ggcactgaaa 2040

ctgctgaaag aatatctgcc agcgtcctac cacgaagggt ctaaaaatcc ggtagcgcgt 2100

gaacgtgttc acagtgcagc gactatcgcg ggtatcgcgt ttgcgaacgc cttcctgggt 2160

gtatgtcact caatggcgca caaactgggt tcccagttcc atattccgca cggtctggca 2220

aacgccctgc tgatttgtaa cgttattcgc tacaatgcga acgacaaccc gaccaagcag 2280

actgcattca gccagtatga ccgtccgcag gctcgccgtc gttatgctga aattgccgac 2340

cacttgggtc tgagcgcacc gggcgaccgt actgctgcta agatcgagaa actgctggca 2400

tggctggaaa cgctgaaagc tgaactgggt attccgaaat ctatccgtga agctggcgtt 2460

caggaagcag acttcctggc gaacgtggat aaactgtctg aagatgcatt cgatgaccag 2520

tgcaccggcg ctaacccgcg ttacccgctg atctccgagc tgaaacagat tctgctggat 2580

acctactacg gtcgtgatta tgtagaaggt gaaactgcag cgaagaaaga agctgctccg 2640

gctaaagctg agaaaaaagc gaaaaaatcc gcttaa 2676

<210> 239

<211> 1443

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 239

atgtccagaa ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc 60

gataataatc ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct 120

cacggctcgc ctgaagatca caaaatgcgc gcggataaag ttcgtgagat tgccgcaaaa 180

ctggggcgtc atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc 240

tttaaagaag gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg 300

ggtaaaggtg aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac 360

gtcgtgcctg gtgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa 420

gttcagggca tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa 480

ggtatcaaca aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca 540

gacattaaga ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt 600

ggcgaagatc tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt 660

gttgccaagg ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga tgacatcatc 720

ctcgcctctg acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg 780

gaactggtcg gcattcagaa agcgttgatc cgtcgtgcgc gtcagctaaa ccgagcggta 840

atcacggcga cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa 900

gtcatggacg tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa 960

actgccgctg ggcagtatcc gtcagaaacc gttgcagcca tggcgcgcgt ttgcctgggt 1020

gcggaaaaaa tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat 1080

gtggaagaag ctattgccat gtcagcaatg tacgcagcta accacctgaa aggcgttacg 1140

gcgatcatca ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct 1200

ggtctgccaa ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat 1260

cgtggcgtta cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa 1320

gcggttaatc tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc 1380

cagggcgacg tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag 1440

taa 1443

<210> 240

<211> 1413

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 240

atgaaaaaga ccaaaattgt ttgcaccatc ggaccgaaaa ccgaatctga agagatgtta 60

gctaaaatgc tggacgctgg catgaacgtt atgcgtctga acttctctca tggtgactat 120

gcagaacacg gtcagcgcat tcagaatctg cgcaacgtga tgagcaaaac tggtaaaacc 180

gccgctatcc tgcttgatac caaaggtccg gaaatccgca ccatgaaact ggaaggcggt 240

aacgacgttt ctctgaaagc tggtcagacc tttactttca ccactgataa atctgttatc 300

ggcaacagcg aaatggttgc ggtaacgtat gaaggtttca ctactgacct gtctgttggc 360

aacaccgtac tggttgacga tggtctgatc ggtatggaag ttaccgccat tgaaggtaac 420

aaagttatct gtaaagtgct gaacaacggt gacctgggcg aaaacaaagg tgtgaacctg 480

cctggcgttt ccattgctct gccagcactg gctgaaaaag acaaacagga cctgatcttt 540

ggttgcgaac aaggcgtaga ctttgttgct gcttccttta ttcgtaagcg ttctgacgtt 600

atcgaaatcc gtgagcacct gaaagcgcac ggcggcgaaa acatccacat catctccaaa 660

atcgaaaacc aggaaggcct caacaacttc gacgaaatcc tcgaagcctc tgacggcatc 720

atggttgcgc gtggcgacct gggtgtagaa atcccggtag aagaagttat cttcgcccag 780

aagatgatga tcgaaaaatg tatccgtgca cgtaaagtcg ttatcactgc gacccagatg 840

ctggattcca tgatcaaaaa cccacgcccg actcgcgcag aagccggtga cgttgcaaac 900

gccatcctcg acggtactga cgcagtgatg ctgtctggtg aatccgcaaa aggtaaatac 960

ccgctggaag cggtttctat catggcgacc atctgcgaac gtaccgaccg cgtgatgaac 1020

agccgtctcg agttcaacaa tgacaaccgt aaactgcgca ttaccgaagc ggtatgccgt 1080

ggtgccgttg aaactgctga aaaactggat gctccgctga tcgtggttgc tactcagggc 1140

ggtaaatctg ctcgcgcagt acgtaaatac ttcccggatg ccaccatcct ggcactgacc 1200

accaacgaaa aaacggctca tcagttggta ctgagcaaag gcgttgtgcc gcagcttgtt 1260

aaagagatca cttctactga tgatttctac cgtctgggta aagaactggc tctgcagagc 1320

ggtctggcac acaaaggtga cgttgtagtt atggtttctg gtgcactggt accgagcggc 1380

actactaaca ccgcatctgt tcacgtcctg taa 1413

<210> 241

<211> 1221

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 241

gtggttgctg aaaaccagcc tgggcacatt gatcaaataa agcagaccaa cgcgggcgcg 60

gtttatcgcc tgattgatca gcttggtcca gtctcgcgta tcgatctttc ccgtctggcg 120

caactggctc ctgccagtat cactaaaatt gtccgtgaga tgctcgaagc acacctggtg 180

caagagctgg aaatcaaaga agcggggaac cgtggccgtc cggcggtggg gctggtggtt 240

gaaactgaag cctggcacta tctttctctg cgcattagtc gcggggagat tttccttgct 300

ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc aggaactggc gttaaaagat 360

gacttgccat tgctggatcg tattatttcc catatcgatc agttttttat ccgccaccag 420

aaaaaacttg agcgtctaac ttcgattgcc ataaccttgc cgggaattat tgatacggaa 480

aatggtattg tacatcgcat gccgttctac gaggatgtaa aagagatgcc gctcggcgag 540

gcgctggagc agcataccgg cgttccggtt tatattcagc atgatatcag cgcatggacg 600

atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg atgtgattca ggtggttatc 660

gatcacaacg tgggggcggg cgtcattacc gatggtcatc tgctacacgc aggcagcagt 720

agtctcgtgg aaataggcca cacacaggtc gacccgtatg ggaaacgctg ttattgcggg 780

aatcacggct gcctcgaaac catcgccagc gtggacagta ttcttgagct ggcacagctg 840

cgtcttaatc aatccatgag ctcgatgtta catggacaac cgttaaccgt ggactcattg 900

tgtcaggcgg cattgcgcgg cgatctactg gcaaaagaca tcattaccgg ggtgggcgcg 960

catgtcgggc gcattcttgc catcatggtg aatttattta acccacaaaa aatactgatt 1020

ggctcaccgt taagtaaagc ggcagatatc ctcttcccgg tcatctcaga cagcatccgt 1080

cagcaggccc ttcctgcgta tagtcagcac atcagcgttg agagtactca gttttctaac 1140

cagggcacga tggcaggcgc tgcactggta aaagacgcga tgtataacgg ttctttgttg 1200

attcgtctgt tgcagggtta a 1221

<210> 242

<211> 2145

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 242

gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc 60

cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc 120

gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac 180

tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc 240

agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa 300

gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag 360

tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag 420

ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc 480

ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat 540

gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa 600

gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgct 660

gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat 720

gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc 780

gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc 840

gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc 900

ggtgccctgc tgctgactgg cggttacgaa atggacgcgc gcatttctaa actgtgcgaa 960

cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacctct 1020

ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg tatcgagaaa 1080

gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct gactgccact 1140

tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac tgaacttgcg 1200

cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca 1260

gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag 1320

atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga aatcgttgat 1380

ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc 1440

atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg tacgctgatg 1500

ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc 1560

atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg 1620

ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat 1680

ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc 1740

ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg tgcaggtagc 1800

gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg 1860

atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg 1920

ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt 1980

aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg gccgatgctg 2040

cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc 2100

tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaa 2145

<210> 243

<211> 1602

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 243

atgactgaac aggcaacaac aaccgatgaa ctggctttca caaggccgta tggcgagcag 60

gagaagcaaa ttcttactgc cgaagcggta gaatttctga ctgagctggt gacgcatttt 120

acgccacaac gcaataaact tctggcagcg cgcattcagc agcagcaaga tattgataac 180

ggaacgttgc ctgattttat ttcggaaaca gcttccattc gcgatgctga ttggaaaatt 240

cgcgggattc ctgcggactt agaagaccgc cgcgtagaga taactggccc ggtagagcgc 300

aagatggtga tcaacgcgct caacgccaat gtgaaagtct ttatggccga tttcgaagat 360

tcactggcac cagactggaa caaagtgatc gacgggcaaa ttaacctgcg tgatgcggtt 420

aacggcacca tcagttacac caatgaagca ggcaaaattt accagctcaa gcccaatcca 480

gcggttttga tttgtcgggt acgcggtctg cacttgccgg aaaaacatgt cacctggcgt 540

ggtgaggcaa tccccggcag cctgtttgat tttgcgctct atttcttcca caactatcag 600

gcactgttgg caaagggcag tggtccctat ttctatctgc cgaaaaccca gtcctggcag 660

gaagcggcct ggtggagcga agtcttcagc tatgcagaag atcgctttaa tctgccgcgc 720

ggcaccatca aggcgacgtt gctgattgaa acgctgcccg ccgtgttcca gatggatgaa 780

atccttcacg cgctgcgtga ccatattgtt ggtctgaact gcggtcgttg ggattacatc 840

ttcagctata tcaaaacgtt gaaaaactat cccgatcgcg tcctgccaga cagacaggca 900

gtgacgatgg ataaaccatt cctgaatgct tactcacgcc tgttgattaa aacctgccat 960

aaacgcggtg cttttgcgat gggcggcatg gcggcgttta ttccgagcaa agatgaagag 1020

cacaataacc aggtgctcaa caaagtaaaa gcggataaat cgctggaagc caataacggt 1080

cacgatggca catggatcgc tcacccaggc cttgcggaca cggcaatggc ggtattcaac 1140

gacattctcg gctcccgtaa aaatcagctt gaagtgatgc gcgaacaaga cgcgccgatt 1200

actgccgatc agctgctggc accttgtgat ggtgaacgca ccgaagaagg tatgcgcgcc 1260

aacattcgcg tggctgtgca gtacatcgaa gcgtggatct ctggcaacgg ctgtgtgccg 1320

atttatggcc tgatggaaga tgcggcgacg gctgaaattt cccgtacctc gatctggcag 1380

tggatccatc atcaaaaaac gttgagcaat ggcaaaccgg tgaccaaagc cttgttccgc 1440

cagatgctgg gcgaagagat gaaagtcatt gccagcgaac tgggcgaaga acgtttctcc 1500

caggggcgtt ttgacgatgc cgcacgcttg atggaacaga tcaccacttc cgatgagtta 1560

attgatttcc tgaccctgcc aggctaccgc ctgttagcgt aa 1602

<210> 244

<211> 825

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 244

atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg 60

actggacagg ttcagtcttt aacgcgtggc ctgaaattac tggagtggat tgccgaatcc 120

aatggcagtg tggcactcac ggaactggcg caacaagccg ggttacccaa ttccacgacc 180

caccgcctgc taaccacgat gcaacagcag ggtttcgtgc gtcaggttgg cgaactggga 240

cattgggcaa tcggcgcaca tgcctttatg gtcggcagca gctttctcca gagccgtaat 300

ttgttagcga ttgttcaccc tatcctgcgc aatctaatgg aagagtctgg cgaaacggtc 360

aatatggcgg tgcttgatca aagcgatcac gaagcgatta ttatcgacca ggtacagtgt 420

acgcatctga tgcgaatgtc cgcgcctatc ggcggtaaat tgccgatgca cgcttccggt 480

gcgggtaaag cctttttagc ccaactgagc gaagaacagg tgacgaagct gctgcaccgc 540

aaagggttac atgcctatac ccacgcaacg ctggtgtctc ctgtgcattt aaaagaagat 600

ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg aggaacatgc actggggcta 660

cgttgccttg cagcgtgtat tttcgatgag caccgtgaac cgtttgccgc aatttctatt 720

tccggaccga tttcacgtat taccgatgac cgcgtgaccg agtttggcgc gatggtgatt 780

aaagcggcga aggaagtgac gctggcgtac ggtggaatgc gctga 825

<210> 245

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失kgtP 1

<400> 245

ggctataccg tctggcaact gacccgtcac taaagacgca tccccttccc 50

<210> 246

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失kgtP 2

<400> 246

aaaagcgacc gacaaaagca tcggattacg gcaggagaca taatggcatg 50

<210> 247

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失llgP 1

<400> 247

gtctgacagg cgtctgggta aaacaatcat taaggaatca tccacgttaa 50

<210> 248

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失llgP 2

<400> 248

agcaacagac tcattacacg atgtgcgtgg actccaggag acctgcaatg 50

<210> 249

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失glcA 1

<400> 249

gatattaacg atcatccggc tttattgatt tacgagacta acatcccggt 50

<210> 250

<211> 50

<212> DNA

<213> 人工的

<220>

<223> 引物缺失glcA 2

<400> 250

aaccttaagt atatcaagca tataaagata ataagagact gaacaatatg 50

Claims

1.一种用于通过发酵产生2,4-二羟基丁酸的经遗传修饰的微生物，其中所述微生物经进一步遗传修饰用于降低细胞内2,4-二羟基丁酸积累，由此优化2,4-二羟基丁酸产生。

2.权利要求1的微生物，其中所述用于降低细胞内2,4-二羟基丁酸积累的遗传修饰是：

i)过表达至少一个编码外排系统的基因；和/或

ii)缺失至少一个编码摄取转运蛋白的基因或弱化其表达。

3.根据权利要求2的微生物，其中所述外排系统选自下组：单羧酸外排系统、甲酸外排系统、乳酸外排系统、苹果酸外排系统、琥珀酸外排系统、芳族羧酸外排系统、它们的功能变体，以及它们的任何组合。

4.根据权利要求2或3的微生物，其中所述外排系统选自下组：

○氨基酸序列SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7、SEQ ID NO:9、SEQ ID NO:11、SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:17和SEQ ID NO:19的单羧酸外排系统，

○氨基酸序列SEQ ID NO:21的甲酸外排系统，

○氨基酸序列SEQ ID NO:23、SEQ ID NO:25、SEQ ID NO:27、SEQ ID NO:29、SEQ IDNO:31、SEQ ID NO:33、SEQ ID NO:35、SEQ ID NO:37、SEQ ID NO:39、SEQ ID NO:41、SEQ IDNO:43、SEQ ID NO:45、SEQ ID NO:47、SEQ ID NO:49、SEQ ID NO:51和SEQ ID NO:53的乳酸外排系统，

○氨基酸序列SEQ ID NO:69和SEQ ID NO:71的芳族羧酸外排系统，

○它们的功能变体，其与所述氨基酸序列具有至少80％序列同一性，和

○它们的任何组合。

5.根据权利要求2至4中任一项的微生物，其中所述外排系统选自下组：

○氨基酸序列SEQ ID NO:1的单羧酸外排系统，

○氨基酸序列SEQ ID NO:23的乳酸外排系统，

○氨基酸序列SEQ ID NO:25的乳酸外排系统，

○它们的任何组合。

6.根据权利要求2的微生物，其中所述摄取转运蛋白选自下组：α-酮戊二酸摄取转运蛋白、乳酸摄取转运蛋白、乙醇酸摄取转运蛋白、乙酸摄取转运蛋白、丙酸摄取转运蛋白、泛酸摄取转运蛋白、琥珀酸和乙酸摄取转运蛋白、乙酰乙酸摄取转运蛋白、葡糖酸摄取转运蛋白，它们的功能变体，和它们的任何组合。

7.根据权利要求2或6的微生物，其中所述摄取转运蛋白选自下组：

○氨基酸序列SEQ ID NO:73的α-酮戊二酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:75的乳酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:77的乙醇酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:79和SEQ ID NO:81的乙酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:83的丙酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:85的泛酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:87的琥珀酸和乙酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:89的乙酰乙酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:91的葡糖酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:93的摄取转运蛋白，

○它们的任何组合。

8.根据权利要求2、6或7的微生物，其中所述摄取转运蛋白选自下组：

○氨基酸序列SEQ ID NO:73的α-酮戊二酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:75的乳酸摄取转运蛋白，

○氨基酸序列SEQ ID NO:77的乙醇酸摄取转运蛋白，

○它们的任何组合。

9.根据权利要求1至8中任一项的微生物，其中所述微生物经遗传修饰以使以下代谢中间产物的至少一个转化为2,4-二羟基丁酸：

iii)1,2,4-丁三醇；

iv)(L)-苹果酸；

v)(L)苹果酰CoA；和

vi)(L)-高丝氨酸。

10.根据权利要求1至9中任一项的微生物，其中所述微生物是属于肠杆菌科(Enterobacteriaceae)、梭菌科(Clostridiaceae)、芽孢杆菌科(Bacillaceae)、链霉菌科(Streptomycetaceae)或棒杆菌科(Corynebacteriaceae)的细菌。

11.根据权利要求10的微生物，其中，所述肠杆菌科细菌是大肠杆菌(E.coli)。

12.一种用于产生2,4-二羟基丁酸的方法，其包括：

a)在包含碳源的培养基中在允许将所述碳源转化为2,4-二羟基丁酸的发酵条件下，培养如权利要求1至11中任一项所述的经遗传修饰的微生物，以及

b)从所述培养基中回收2,4-二羟基丁酸。