CN107002019A - 产生3‑羟基丙酸的重组酵母和使用其产生3‑羟基丙酸的方法 - Google Patents

产生3‑羟基丙酸的重组酵母和使用其产生3‑羟基丙酸的方法 Download PDF

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CN107002019A
CN107002019A CN201580052598.1A CN201580052598A CN107002019A CN 107002019 A CN107002019 A CN 107002019A CN 201580052598 A CN201580052598 A CN 201580052598A CN 107002019 A CN107002019 A CN 107002019A
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朴重珉
朴宰演
朴佑赞
李相珉
徐荣彬
欧佳·梅里亚
科维史度恩·欧蒂
康利·安德鲁
勒门·梅里亚
罗胡恩·劳拉
朱赫恩·宝拉
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Abstract

本发明提供了产生3‑羟基丙酸(3‑HP)的重组酵母以及使用其产生3‑HP的方法，更具体地，本发明提供了一种产生3‑HP的重组酵母，该重组酵母包含外源AADH基因、内源ACC基因或外源ACC基因、外源MCR基因和外源HPDH基因，并通过[丙酮酸→乙醛→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3‑HP]生物合成途径产生3‑HP；以及使用该重组酵母制备3‑HP的方法。

Description

产生3-羟基丙酸的重组酵母和使用其产生3-羟基丙酸的方法
技术领域
本发明涉及产生3-羟基丙酸(3-HP)的重组酵母和使用其产生3-HP的方法，更具体地，涉及产生3-HP的重组酵母，其包含编码AADH的外源基因、编码ACC的内源基因或外源基因、编码MCR的外源基因和编码HPDH的外源基因，并通过[丙酮酸(Pyruvate)→乙醛→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3-HP]的生物合成途径产生3-HP，以及使用该重组酵母产生3-HP的方法。
背景技术
3-HP(3-羟基丙酸，C3)是乳酸(2-羟基丙酸)的异构体，在其两端具有羧酸基和羟基，因此是能够转化成各种化学品(如1,3-丙二醇、丙烯酸、丙烯酰胺、聚合物等)的有用材料。实际上，由于上述原因，2004年3-HP被美国能源部选为能够由生物质生产的有前途的化学品之一。特别地，作为用于涂料、粘合剂、添加剂、尿布等的高度市场化的材料，丙烯酸是3-HP的主要应用形式。3-HP理论上可以通过发酵从诸如葡萄糖的各种生物质中以100％的收率生产，使用微生物的发酵工艺适合于满足对生态环境友好和可再生材料的需求。
还没有利用生物质商业生产3-HP的情况，但已经对各种方法进行了研究，获得经济的3-HP产生菌株成为主要障碍。细菌被公认为是产生有机酸的代表性微生物，并广泛用于工业如食品工业等。然而，将使用工业细菌(如大肠杆菌)的有机酸生产应用于大规模化学工业(如3-HP生产)存在缺点。随着有机酸的产量增加，氢化形式的酸增加，酸性增加(pH降低)，从而大多数大肠杆菌的活性降低。在生产高浓度的有机酸的情况下，细菌需要碱如氢氧化钠(NaOH)和氢氧化铵(NH4OH)来保持中性pH。这导致依赖于注入的碱的发酵过程的成本增加，使提取和分离过程难以进行或者使成本显著增加。
在最近的将有机酸生产应用于化学工业的情况中，使用酵母从葡萄糖生产有机酸。与细菌相比，酵母对有机酸具有高耐受性，因此即使在高酸性(低pH)下酵母的活性也不会被显著抑制，使得酵母成为更合适的用于生产有机酸的宿主。特别地，酵母已经被常规地、广泛地用作用于生产烈酒、工业乙醇等的工业生物催化剂，酵母可以被大规模地培养并且可以不被噬菌体污染，使得酵母与细菌相比在化学工业中的适用性更加优异。酵母具有产生有机酸的优点，但是使用酵母产生有机酸如3-HP存在几个缺点。首先，与细菌相比，对酵母进行基因修饰更为困难，为了表达特定的代谢酶，应当鉴定用于表达代谢途径以及特定代谢酶的亚细胞位置，这是由于其细胞形状与细菌不同，被分为细胞内结构体，例如线粒体、过氧化物酶体等。例如，代表性代谢中间体如乙酰辅酶A主要在酵母的线粒体中产生。然而，如果在胞质溶胶中产生目标产物，则也需要在胞质溶胶中产生乙酰辅酶A的方法。此外，由于在真菌界存在大量不同的酵母，并且所有酵母都不满足对高生产率、耐有机酸性和大规模培养的要求，因此应当有效地选择适于产生有机酸的宿主。
已知一些酵母有效地从作为己糖的葡萄糖产生乙醇，一些酵母种类也产生有机酸。在进行修饰以使用微生物产生目标产物时，重要的是保持代谢反应的氧化和还原的整体平衡，并且由葡萄糖发酵生产乙醇的代谢反应是得到适当维持的反应。即使在基因修饰酵母以产生有机酸的情况下，也应当适当地保持上述平衡，并且在通过修饰酵母来生产乳酸的情况下，平衡地引入另一还原反应的乳酸脱氢酶(LDH)用于补充从乙醛生产乙醇的还原反应是重要的。
已知在少数微生物例如橙色绿屈挠菌(Chloroflexus aurantiacus)中产生少量3-HP，并且3-HP部分地由微生物如粪产碱杆菌(Alcaligenes faecalis)中的二甲基磺基丙酸分解过程或酵母中尿嘧啶的分解过程形成。通过发现如上所述的自然界中存在的3-HP，已经进行了3-HP代谢途径和相应的酶的研究，并且基于最近的研究，进行了对以下技术的研究：通过在大肠杆菌中引入3-HP生物合成所需的基因来产生3-HP或PHA的技术，PHA为3-HP的聚合物形式。另外，为了使在自然界中仅作为代谢中间体存在或仅少量产生的3-HP的生产率和产量最大化，必须使用诸如代谢工程技术、系统生物学技术、合成生物学技术等的技术。
根据代谢工程技术的发展，预测使用微生物生产各种物质的生产途径成为可能，从葡萄糖生产3-HP的途径按照代谢中间体可以大致分为丙烯酰辅酶A途径、β-丙氨酸途径、丙二酰辅酶A途径和甘油途径。
丙烯酰辅酶A途径是指将从葡萄糖糖酵解获得的丙酮酸或磷酸烯醇丙酮酸(PEP)经由乳酸(lactate)或β-丙氨酸转化为丙烯酰辅酶A，然后通过水合反应和还原反应将丙烯酰辅酶A转化为3-HP的代谢途径(丙酮酸或PEP→乳酸或β-丙氨酸→丙烯酰辅酶A→3-HP)。丙烯酰辅酶A是在丙酸的分解过程中观察到的代谢物，并且由于形成该代谢物的吉布斯自由能值为正，所以正向反应是不利的反应。此外，丙烯酰辅酶A硫酯酶的底物特异性低，使得丙烯酰辅酶A途径不适合作为大规模生产3-HP的代谢途径。
β-丙氨酸途径是指通过转氨基反应将丙酮酸或草酰乙酸转化为氨基酸，最后通过转氨基反应经由β-丙氨酸转化为3-HP的代谢途径(丙酮酸或草酰乙酸→氨基酸→β-丙氨酸→3-HP；US2012/0135481A1)。由于β-丙氨酸向3-HP转化的转氨基反应经由对微生物具有高毒性的丙二酸半醛进行，因此需要具有高活性的3-HP脱氢酶。另外，通常，由于转氨基反应形成稳态的氨基酸分子结构的自由基形式，所以该反应的酶具有用于减轻自由基的反应性的结构。由于该自由基对氧具有强的反应性，因此对于平稳的转氨基反应，基本上需要厌氧条件或用于稳定自由基分子的辅酶。
丙二酰辅酶A途径是通过羧化将乙酰辅酶A转化为丙二酰辅酶A，然后通过还原反应将丙二酰辅酶A转化为3-HP的代谢途径(乙酰辅酶A→丙二酰辅酶A→3-HP)，甘油途径是将葡萄糖转化为甘油，通过脱水反应将甘油转化为3-羟基丙醛，然后将3-羟基丙醛转化为3-HP的代谢途径(葡萄糖→甘油→3-羟基丙醛→3-HP)。由于丙二酰辅酶A途径和甘油途径经由通常由诸如大肠杆菌等的微生物产生的中间体进行，因此主要将这些途径作为3-HP生产途径进行研究(US 2013/0071893 A1)。由于可以通过丙二酸(malonate)还原酶和3-HP脱氢酶将丙二酰辅酶A转化为3-HP，并且可以通过甘油脱水酶和醛脱氢酶将甘油转化为3-HP，使用修饰的大肠杆菌将葡萄糖或甘油转化为3-HP的方法是众所周知的。与转氨基反应类似，甘油的脱水反应是伴随有自由基的反应，基本上需要辅酶B12以在氧的存在下进行反应。
考虑到工业发酵，由于辅酶(如辅酶B12)的成本，难以使用其作为培养基的物质，并且微生物(例如酵母)不能在细胞中生物合成或吸收相应的物质，因此β-丙氨酸途径或甘油途径不适合作为使用酵母产生3-HP的代谢途径。最近，已经报道了修饰关键酶以克服该问题的研究。[US 7,655,451 B2]
丙二酰辅酶A由胞质溶胶中的乙酰辅酶A合成，从而可以还原成3-HP。在细菌如大肠杆菌的情况下，乙酰辅酶A由胞质溶胶中的丙酮酸形成，因此可用作TCA循环或其它代谢反应的底物。然而，如上所述，在具有独立的亚细胞区室的酵母中，通常在线粒体中合成乙酰辅酶A并将其用作TCA循环的底物，并且胞质溶胶中的乙酰辅酶A通过乙酸产生反应或柠檬酸循环反应来产生，乙酸产生反应是乙醇产生反应的副反应。从乙酸或柠檬酸产生乙酰辅酶A的所有反应都是消耗ATP的反应，并且由于与细菌相比，酵母还消耗能量以在胞质溶胶中获得乙酰辅酶A，所以酵母在能量学方面是不利的。
在酵母中，诸如胞质溶胶的还原状态、蛋白质合成后的折叠、密码子使用等的环境与细菌中的环境不同，使得在表达来自细菌的外源酶时，不显示其活性或活性可能显著降低。此外，由于氧或其它金属离子的存在或不存在可以显著改变所述活性，即使在具有相同功能的外源酶的情况下，其在酵母中的表达结果会根据酶的来源而不同。实际上，在木糖代谢中的重要酶即木糖异构酶(XI)的情况下，当在酵母中表达来自细菌的XI时，通常其活性明显较低，但是证明了通过引入来自厌氧真菌的XI，酵母被成功修饰从而以相对高的活性进行木糖代谢。因此，在酵母中引入来自细菌或古生菌的代谢途径(如丙二酰辅酶A途径)的情况下，应通过发挥相同功能的各种来源的基因来获得具有高活性的基因。
存在与基因修饰各种酵母菌株中的酿酒酵母(Saccharomyces cerevisiae)以产生3-HP的方法相关的各种文献和专利，但是在酿酒酵母的情况下，由于3-HP通过[丙酮酸→乙醛→乙酸→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3-HP]途径生产，存在该产生3-HP的代谢途径复杂、3-HP的生产率相对低的问题(US2010/0248233A1；Y.Chen等人，MetabolicEngineering，22:104-109,2014)。
因此，作为解决上述问题的努力的结果，为了克服酵母在获得胞质溶胶中乙酰辅酶A的过程中消耗ATP的缺点，本发明人想到更短的代谢途径：[丙酮酸→乙醛→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3-HP]，该代谢途径不经过乙酸中间体而直接将乙醛转化为乙酰辅酶A，并且证实在使用包含该途径的重组酵母的情况下，与使用大肠杆菌的情况不同，不仅减少了pH调节物质的使用(从而减少了盐的产生)，而且即使在低的pH下也可以以高浓度和高产率从生物质产生3-HP，从而完成本发明。
发明内容
技术问题
本发明的目的是提供包含[丙酮酸→乙醛→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3-HP]的主动3-HP生物合成途径的重组酵母，其不经由乙酸而将乙醛直接转化为乙酰辅酶A。
本发明的另一个目的是提供使用该重组酵母产生3-HP的方法。
问题的解决方案
为了实现上述目的，本发明提供了一种包含[丙酮酸→乙醛→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3-HP]的主动3-HP生物合成途径的重组酵母，其中该酵母包含：编码AADH的外源基因；编码ACC的内源基因或外源基因；编码MCR的外源基因；和编码HPDH的外源基因。
在本发明中，所述酵母是耐酸的，并且选自例如酵母属(Saccharomyces)、Kazachstania属和假丝酵母(Candida)属。特别感兴趣的酵母种包括酿酒酵母(Saccharomyces cerevisiae)、Kazachstania exigua、Kazachstania bulderi和小假丝酵母(Candida humilis)，但种类不仅限于此。
此外，本发明提供了制备3-HP的方法，其包括：(a)在包含至少一种碳源的培养基中培养权利要求1-10中任一项所述的重组酵母，从而产生3-HP；以及(b)从培养物中分离3-HP。
附图说明
图1示出了本发明的重组酵母从葡萄糖产生3-HP的途径(修饰的丙二酰辅酶A代谢途径)和主要的酶。
图2示出了乙酰辅酶A羧化酶的ACC1活性的相对排名。
图3示出了对古细菌MCR变体的相对活性(左图)和表达水平(SDS-PAGE)(右图)的确认结果。
图4示出了用于表达与3-HP途径相关的酶的酵母表达质粒pSK-084和pSK-085。
图5示出了对可以影响3-HP生产水平的培养条件的测试结果。
图6示出了使用补料分批(片剂掺加(tablet spiking))培养条件时更成熟的3-HP生产菌株的3-HP生产量。
具体实施方式
除非另有定义，本文使用的所有技术和科学术语具有与本发明所属领域的技术人员通常理解的相同的含义。通常，本文使用的命名法是本领域公知的并且通常使用的。
通常，在酵母中，由于乙酰辅酶A通过[乙醛→乙酸→乙酰辅酶A]途径制备，并且在酵母的胞质溶胶中产生乙酸，因此在生产乙酰辅酶A的过程中ATP通过转化为AMP而被消耗掉(图1；Y.Chen等人,Metabolic Engineering,22:104-109,2014)。
然而，在本发明中，设计并应用了一种途径(图1)，在产生胞质溶胶中的乙酰辅酶A的过程中，该途径通过不经由乙酸而由乙醛直接制备乙酰辅酶A来克服和改善酵母消耗ATP的缺点。结果，在使用本发明的重组酵母的情况下，证明了即使在低pH下，也以高浓度和高产率由葡萄糖产生3-HP。
因此，一方面，本发明涉及包含[丙酮酸→乙醛→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3-HP]的主动3-HP生物合成途径的重组酵母，其中该酵母包含：编码AADH的外源基因；编码ACC的内源基因或外源基因；编码MCR的外源基因；和编码HPDH的外源基因。
[丙酮酸→乙醛→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3-HP]的3-HP生物合成途径是从诸如葡萄糖等的碳源产生3-HP的途径。“丙酮酸→乙醛”是指使用丙酮酸脱羧酶(PDC)由丙酮酸生产乙醛而不产生中间体的途径；(ii)“乙醛→乙酰辅酶A”是指使用乙酰化乙醛脱氢酶(acetylating acetaldehyde dehydrogenase，AADH)由乙醛生产乙酰辅酶A而不产生诸如乙酸的中间体的途径；(iii)“乙酰辅酶A→丙二酰辅酶A”是指使用乙酰辅酶A羧化酶(ACC)由乙酰辅酶A生产丙二酰辅酶A而不产生中间体的途径；(iv)“丙二酰辅酶A→3-HP或丙二酰辅酶A→丙二酸半醛→3-HP”是指使用双功能丙二酰辅酶A还原酶(MCR)由丙二酰辅酶A生产3-HP而不产生中间体的途径，或通过使用单功能丙二酰辅酶A还原酶生物合成丙二酸半醛从而由丙二酸半醛产生3-HP的途径(图1)。
在本发明中，PDC基因不是工程化的，但是可以例如通过扩增酵母中存在的PDC基因、通过应用本技术领域熟知的现有技术来工程化PDC基因以增加3-HP生产率。
在本发明的一个示例性实施方案中，通过生物信息学基因组挖掘途径酶候选物来提取编码具有特殊功能的途径酶的基因。
本发明包括编码AADH的外源基因。在一个实施方案中，编码AADH的基因为编码下述AADH的核酸：AADH具有与选自以下表1-3中所示的氨基酸序列的AADH氨基酸序列具有至少60％，优选至少70％、80％、90％、91％、92％、93％、94％、95％、96％、97％、99％或100％的序列同一性的氨基酸序列，但编码AADH的基因不限于此，只要其具有由乙醛生物合成乙酰辅酶A的功能即可。
表1
AADH(adhE型)
表2
AADH(eutE型)
表3
AADH
本发明包括编码ACC的基因。在一个实施方案中，编码ACC的基因为编码下述ACC的核酸，该ACC具有与选自下表4所示的氨基酸序列的ACC氨基酸序列具有至少60％，优选至少70％、80％、90％、91％、92％、93％、94％、95％、96％、97％、99％或100％的序列同一性的氨基酸序列，但编码ACC的基因不限于此，只要其具有由乙酰辅酶A生物合成丙二酰辅酶A的功能即可。
表4
ACC(真核多结构域型)
本发明包括编码MCR的基因。在一个实施方案中，MCR可以是双功能MCR，其具有将丙二酰辅酶A转化为丙二酸半醛的功能和将丙二酸半醛转化为3-HP的功能；或者是单功能MCR，其具有将丙二酰辅酶A转化为丙二酸半醛的功能。
在本发明中，编码所述双功能MCR的基因为编码下述MCR的核酸：MCR具有与选自下表5中所示的氨基酸序列的MCR氨基酸序列具有至少60％，优选至少70％、80％、90％、91％、92％、93％、94％、95％、96％、97％、99％或100％的序列同一性的氨基酸序列，但编码所述双功能的基因不限于此，只要其同时具有将丙二酰辅酶A转化为丙二酸半醛的功能和将丙二酸半醛转化为3-HP的功能即可。
表5
MCR(双功能型)
在另一个实施方案中，所述MCR基因可以是具有将丙二酰辅酶A转化为丙二酸半醛的功能的单功能基因，可以进一步包含编码可以将丙二酸半醛转化为3-HP的酶的基因。
在本发明中，所述编码单功能MCR的基因为编码下述MCR的核酸：该MCR具有与选自下表6中所示的氨基酸序列的MCR氨基酸序列具有至少60％，优选至少70％、80％、90％、91％、92％、93％、94％、95％、96％、97％、99％或100％的序列同一性的氨基酸序列，但编码所述单功能MCR的基因不限于此，只要其具有将丙二酰辅酶A转化为丙二酸半醛的功能即可。
表6
MCR
在本发明中，编码将丙二酸半醛转化为3-HP的酶的基因是编码下述酶的核酸：该酶具有与选自下表7-10所示的氨基酸序列的HPDH、HIBADH、HBDH或BDH氨基酸序列具有至少60％，优选至少70％、80％、90％、91％、92％、93％、94％、95％、96％、97％、99％或100％的序列同一性的氨基酸序列，但所述编码酶的基因不限于此，只要该蛋白编码基因具有从丙二酸半醛生物合成3-HP的功能即可。
下表7中所示的HPDH氨基酸序列、下表8中所示的HIBADH氨基酸序列、下表9中所示的HBDH氨基酸序列和下表10中所示的BDH-氨基酸序列为具有从丙二酸半醛生物合成3-HP的功能的蛋白质的氨基酸序列。
表7
HPDH
表8
HIBADH
表9
HBDH
表10
BDH
在本发明的一个具体实施方案中，包含主动3-HP生物合成途径的重组酵母可以具有表11中所列的基因，例如编码具有选自SEQ ID NO:74-98的氨基酸序列的AADH的外源基因、编码具有选自SEQ ID NO:99-106的氨基酸序列的ACC的内源基因或外源基因、编码具有选自SEQ ID NO:107-116的氨基酸序列的MCR的外源基因以及编码具有选自SEQ ID NO：117-144的氨基酸序列的HPDH的外源基因。
表11
在本发明中，酵母可以是耐酸的，并且所述耐酸酵母可以选自例如酵母属、Kazachstania属和假丝酵母属。特别感兴趣的酵母种包括酿酒酵母、Kazachstaniaexigua、Kazachstania bulderi和小假丝酵母，但不限于此。
根据本发明，重组酵母可以是耐酸的，为了制备耐酸重组酵母，优选使用对有机酸(特别是3-HP和/或制备3-HP时作为副产物产生有机酸)具有耐酸性的酵母宿主。
耐酸性酵母可以选自酿酒酵母、Kazachstania exigua、Kazachstania bulderi和小假丝酵母，但不限于此。
本说明书中使用的术语“耐酸性酵母”是指对有机酸如3-HP等具有耐酸性的酵母，耐酸性可以通过确认在含有各种浓度的有机酸的培养基上的生长来评价。在这种情况下，“耐酸性酵母”可以是当在含有高浓度有机酸的培养基中生长时与一般酵母相比表现出高生长速率和高生物质消耗速率的酵母。
根据本发明，耐酸性酵母可以是在含有超过1M或更多有机酸(特别是3-HP)的培养基中、在小于有机酸(特别是3-HP)的pKa值的pH下，与在不含有机酸的培养基中生长的酵母相比，能够维持至少10％的葡萄糖(或类似物)消耗速率或至少10％的比生长速率的酵母。根据本发明，耐酸性酵母可以是在pH 2-4下，与在pH 7下相比，可以维持至少10％的葡萄糖(或类似物)消耗速率或至少10％的比生长速率的酵母。
本发明的经基因修饰的微生物可以通过将基因插入微生物的染色体或将经修饰的载体引入微生物中来制备。
通常使用DNA引入效率高且引入的DNA的表达效率高的宿主作为所述经修饰的微生物，在本发明的一个示例性实施方案中，使用酵母，但不限于此，只要能充分表达靶DNA，可以使用任何种类的微生物。
所述经修饰的微生物可以通过任何转化方法制备。“转化”是指将DNA引入宿主，从而DNA能够作为染色体的因子或通过整合染色体而复制，这是人为引起基因改变的现象。在常见的转化方法中，存在电穿孔、乙酸锂-PEG方法等。
另外，在本发明中，可以使用一般公知的基因工程方法作为向宿主微生物的染色体中引入基因的方法，例如存在使用逆转录病毒载体、腺病毒载体、腺伴随病毒载体、单纯疱疹病毒载体、痘病毒载体、慢病毒载体、非病毒载体等的方法。“载体”是指包含待可操作地连接到合适的控制序列的、能够在宿主内部表达DNA的DNA序列的DNA构建体。载体可以是质粒、噬菌体颗粒或简单的潜在基因组插入片段。当载体转化到合适的宿主中时，其可以被复制或发挥功能而不管宿主基因组如何，或在一些情况下，其可以整合到基因组本身中。质粒是最常用作载体的类型。
可用于该目的的典型质粒载体的结构包含(a)允许有效进行复制以使每个宿主细胞包含质粒载体的复制起点，(b)抗生素抗性基因或营养缺陷型标记基因，其允许选择用质粒载体转化的宿主细胞，和(c)可以插入外源DNA片段的限制酶限制性位点。即使不存在合适的限制酶限制性位点，当使用根据一般方法的接头或合成的寡核苷酸衔接子(adaptor)时，可以容易地将载体和外源DNA连接。
当核酸与其他核酸序列以功能关系排列时，其是“可操作地连接的”。它可以是：当适当分子(例如转录激活蛋白)连接到控制序列时在能够进行基因表达的过程中连接的基因和控制序列。例如，当表达参与多肽分泌的前蛋白时，前序列或分泌前导序列的DNA可操作地连接至多肽的DNA；在影响序列的转录时启动子或增强子可操作地连接至编码序列；当影响序列的转录时，核糖体结合结构域可操作地连接至编码序列；或当核糖体结合结构域被设置为易于被翻译时，其与编码序列可操作地连接。
通常，“可操作地连接”是指连接的DNA序列的接触，或分泌前导序列在前导框中接触和呈递。然而，增强子不需要接触。增强子序列的连接通过在方便的限制酶位点处的连接来进行。当不存在该结构域时，使用根据一般方法的合成寡核苷酸衔接子或接头。
当然，应当理解，所有载体都不能等同地发挥作用以表达根据本发明的DNA序列。同样地，所有宿主细胞对于相同的表达系统也不是等同地起作用。然而，在不脱离本发明的范围且没有过度的实验的情况下，本领域技术人员可以适当地选择载体、表达控制序列和宿主细胞。例如，在选择载体时，必须考虑宿主细胞。这是因为载体应当在宿主细胞中复制。此外，还应当考虑复制数量和控制载体的复制数量的能力以及由载体编码的其它蛋白质(例如抗生素标记物)的表达。
在另一方面，本发明涉及制备3-HP的方法，其包括：(a)在包含至少一种碳源的培养基中培养权利要求1-10中任一项所述的重组酵母，从而产生3-HP；以及(b)从培养物中分离3-HP。
在本发明中，碳源可以是选自葡萄糖、木糖、阿拉伯糖、蔗糖、果糖、纤维素、葡萄糖低聚物和甘油中的一种或多种，但不限于此。
在本发明中，培养优选在微生物如大肠杆菌不再工作(例如产生代谢物等)的条件下进行。在实施方案中，在pH 1.0-6.5，优选在pH 1.0-6.0，更优选在pH 2.6-4.0下进行培养，但不限于此。
具体实施方式
在下文中，将参考以下实施例更详细地描述本发明。然而，以举例的方式提供以下实施例是为了容易地解释对本发明技术精神的描述和本发明技术精神的范围。因此，本发明的范围不限于此或不由此改变。另外，本发明所属领域的技术人员可根据本说明书的描述进行各种修改和改变。
实施例1基于对3-HP的耐受性选择宿主酵母菌株
生产3-HP的生物体的基本特征是对高浓度3-HP的良好耐受性，这使得在发酵期间产物能够积累，同时菌株性能损失最小。对一个718个野生型酵母菌株组成的大的、多样化的组(set)进行筛选以鉴定在低pH下能够耐受高浓度3-HP并且在这些条件下可以生长和代谢葡萄糖的那些菌株(表12)。
最初使用许多基于琼脂板和液体培养基微量滴定板的生长测定来筛选整组菌株的耐酸性。然后，评价菌株亚组(subset)在摇瓶培养中在低pH下耐受大量3-HP的能力。这些筛选方法中没有一个孤立地是工业环境中3-HP耐受性的完美指标，但是许多方法的组合提供了一种全面而稳健的确定有前途的产生3-HP酵母菌株的方法。
最初，在含有不同量的3-HP的固体YPD基琼脂培养基上评估718个酵母菌株的生长，其中3-HP的量为：0g/L 3-HP(pH 6.62)、50g/L 3-HP(pH 3.44)、75g/L 3-HP(pH 3.28)、100g/L 3-HP(pH 3.17)和125g/L 3-HP(pH 3.08)。然后基于它们在该筛选测定中耐受不同量的3-HP的能力对菌株进行评分。
然后使用可以自动孵育、摇动和测量培养物浊度的Bioscreen C机器在不存在3-HP(基于SCD的培养基，0g/L 3-HP，pH 6.0)或存在3-HP(基于SCD的培养基，70g/L 3-HP，pH3.5)的情况下在微量滴定板液体培养物中评估718个酵母菌株的生长。然后采用现有的用于建模微生物生长曲线的软件在每个实验条件下为每个菌株确定滞后期、最大生长速率和最终细胞密度。对于该筛选测定，基于其在不存在3-HP时的最大生长速率、其在3-HP存在下的最大生长速率和这两个最大生长速率值之间的相对差对每个菌株进行评分。
使用液体处理自动控制仪器(robot)，在微量滴定板中在含有85g/L 3-HP(pH3.5)的基于YPD的液体培养基中评估718个酵母菌株的生长和葡萄糖利用率。使用自动化工作流程(work-flow)来接种生长平板、在指定时间点稀释样品用于OD测量并离心和收集上清用于HPLC分析，HPLC分析用于测定指定时间点处残留葡萄糖量。与Bioscreen C生长测定相反，自动操作(robotic)微量滴定板生长测定允许实现更多的通气和更高的最大细胞密度，同时还允许评估葡萄糖利用率，而不像之前的两种测定一样仅评估生长速率。对于该筛选测定，基于在3-HP存在下获得的菌株最大细胞密度和菌株在3-HP存在下在低pH条件下消耗葡萄糖的能力对每个菌株进行评分。
将来自各种3-HP耐受性评估测定的个体评分一起取平均值以获得每种菌株的最终3-HP耐受性评分(表12)。这些筛选表明，在低pH和不同条件下，以下酵母种类具有对3-HP的良好的一般耐受性：蜂生假丝酵母(Candida apicola)、小假丝酵母、东方伊萨酵母(Issatchenkia orientalis)、Kazachstania bulderi、Kazachstania exigua、膜璞毕赤酵母(Pichia membranifaciens)、酿酒酵母和解脂耶氏酵母(Yarrowia lipolytica)。
然后在摇瓶培养中在低pH下进一步分析这8种3-HP耐受性酵母种对3-HP的耐受性。对于这些培养(定义的基于SCD的培养基，高初始生物质，低通风)，在以下不同的pH值和不同水平的3-HP存在下评估菌株生长、消耗葡萄糖和产生乙醇的能力：100g/L 3-HP(pH4.0)、100g/L 3-HP(pH 3.5)、100g/L 3-HP(pH 3.0)和80g/L 3-HP(pH 2.6)。这些摇瓶培养显示某些小假丝酵母(C.humilis)、K.bulderi、K.exigua和酿酒酵母菌株在低pH下对高水平的3-HP具有非常强的耐受性，因为在各种酵母菌株中它们在这些苛刻条件下具有最快的葡萄糖利用率、生物质产生速率和乙醇产生速率。另一方面，在这些非常受限的生长条件下，蜂生假丝酵母、东方伊萨酵母、膜璞毕赤酵母和解脂耶氏酵母菌株不能良好地表现。这些使用各种摇瓶培养的详细后续分析证实某些小假丝酵母、K.bulderi、K.exigua和酿酒酵母菌株显示出作为3-HP产生宿主的巨大潜力，因为它们显示出在工业相关条件下对3-HP的高的天然耐受性。
表12
酵母菌株对3-HP的耐受性
实施例2生物信息学基因组挖掘以获得途径酶候选物
进行基于同源性的数据库搜索以鉴定用于特定功能的候选酶。在每种情况下，用多个查询序列搜索数据库。此外，相关蛋白家族的成员基于InterPro结构域注释从Uniprot/SwissProt中检索。使用blastp针对Uniprot(SwissProt和TrEMBL)和GenBank蛋白质数据库(nr，pat和env_nr)，以及使用tblastn针对GenBank核苷酸数据库(tsa_nt，env_nt和pat)进行基于同源性的搜索。提取E值小于1e-30的序列；然而，在一些情况下，对E值小于1e-80的序列进行额外的分析。使用查询序列作为翻译中的指导，用GeneWise将命中的核苷酸翻译成蛋白质序列。对于GeneWise而言，翻译长ACC序列的任务太困难，因此，从blast-xml输出中提取蛋白质序列(与查询序列匹配的部分)。为了去除冗余序列，使用BLASTCLUST或CD-HIT将检索的序列集中(clustered)成含有彼此具有80％以上同一性的序列的集合(cluster)。从每个集合仅保留一个代表性序列。将非冗余的序列组要么与蛋白质家族的PFAM结构域比对要么通过使用MAFFT进行比对。在蛋白质家族不与任何PFAM相关的情况下或者如果蛋白质的序列被分割成几个PFAM结构域，则创建MAFFT的全局比对。基于使用PHYLIP或FASTTREE的多序列比对生成系统发生树。该树用E.C.数、生物体名称、blast E值注释，并使用Geneious软件可视化。
2-1：乙酰化乙醛脱氢酶
乙醛还原为乙酰辅酶A可以通过乙酰化乙醛脱氢酶(AADH，E.C.1.2.1.10)完成。AADH可以分为三组功能同源物(Wei等人，Nat.Commun.4：2580,2013)，包括1)具有AADH和醇脱氢酶活性的双功能蛋白(大肠杆菌adhE型基因，GenBank No.：NP_415757，查询序列)，2)参与乙醇胺分解代谢的蛋白质(大肠杆菌eutE型基因，GenBank No.：AAG57564，查询序列)和3)双功能蛋白质，其是参与4-羟基-2-酮戊酸分解代谢的醛缩酶-脱氢酶复合物的一部分(大肠杆菌mphF型基因，GenBank No.：NP_414885)。特别感兴趣的是第1组(adhE型)和第2组(eutE型)的酶。
AdhE蛋白的N-末端结构域与醛:NAD+氧化还原酶高度同源，而C末端区与Fe2+-依赖性乙醇:NAD+氧化还原酶家族同源(Membrillo-Hernandez等人，J.Biol.Chem.275:33869-33875,2000)。乙酰化乙醛脱氢酶活性也可以通过去除醇脱氢酶结构域来截短双功能AdhE蛋白从而仅具有N-末端醛还原酶结构域而引入细胞。基于生物信息学分析和序列同源性推断具有这种双功能AADH活性的其他基因。各种基因总结在表1中。
许多肠杆菌可以利用乙醇胺作为碳源和氮源(Stojiljkovic等人，J.Bacteriol.177:1357-1366,1995)。该分解代谢途径包括通过乙酰化乙醛脱氢酶EutE将乙醛转化为乙酰辅酶A的步骤。基于生物信息学分析和序列同源性推断具有这种AADH活性的新基因。各种基因总结在表2中。
此外，基于生物信息学分析和与adhE以及eutE型基因的序列同源性，存在一组注释为醛脱氢酶的基因，可以推断其具有AADH活性。各种基因总结在表3中。
2-2：真核乙酰辅酶A羧化酶
乙酰辅酶A羧化酶(ACC，EC 6.4.1.2)是多功能生物素依赖性羧化酶，是脂肪酸生物合成的关键酶。它使用辅因子ATP和生物素催化乙酰辅酶A转化为丙二酰辅酶A。该反应分两步进行。首先，生物素羧化酶催化生物素与碳酸氢盐的ATP依赖性羧化。然后，羧基转移酶将羧基从生物素转移到乙酰辅酶A以形成丙二酰辅酶A。真核生物酶是大的多结构域酶，而相应的原核生物酶由由不同基因编码的多个亚基组成。
ACC的活性在转录水平上以及在转录后水平上受控(例如通过磷酸化和聚集)以维持乙酰辅酶A动态平衡。在不同酵母种中的ACC工程化导致ACC活性增加和丙二酰辅酶A衍生产物的产量增加。已证明或假定在酿酒酵母(GenBank No.：CAA96294.1，查询序列)、解脂耶氏酵母(GenBank No.：XP_501721.1，查询序列)和卷枝毛霉(Mucor circinelloides)(GenBank No.：EPB82652.1，查询序列)中存在编码具有ACC活性的酶的基因。在Kazachstania exigua(SEQ ID NO：1)和小假丝酵母(SEQ ID NO：2)的新测序的基因组中鉴定候选乙酰辅酶A羧化酶基因并克隆到我们的酵母表达质粒中。基于生物信息学分析和序列同源性推断具有ACC活性的其它基因。各种真核多结构域ACC基因总结在表4中。
2-3：双功能丙二酰辅酶A还原酶
丙二酰辅酶A还原成3-HP(经由丙二酸半醛中间体)可以通过大的双功能丙二酰辅酶A还原酶完成，其具有C末端醛脱氢酶结构域和N-末端醇脱氢酶结构域的双功能。具有这种活性的、高度底物特异性的、NADPH依赖性酶的特征在于参与称为3-羟基丙酸循环的自养CO2固定途径的光营养型绿色无硫细菌橙色绿屈挠菌(Chloroflexus aurantiacus)(GenBank No.：AAS20429.1；查询序列)(Hugler等人，J.Bacteriol.184:2404-2410,2002)。基于生物信息学分析和序列同源性推断具有该双功能丙二酰辅酶A还原酶活性的其他基因。各种基因总结在表5中。
2-4：丙二酰辅酶A还原酶
与上面讨论的双功能丙二酰辅酶A还原酶相反，丙二酰辅酶A也可以通过两种单独的酶催化成3-HP。通过该途径，丙二酰辅酶A首先通过丙二酰辅酶A还原酶(MCR；EC1.2.1.75)或辅酶A酰化丙二酸半醛脱氢酶还原为丙二酸半醛，然后通过3-羟基丙酸脱氢酶(3-HPDH；EC 1.1.1.59或EC 1.1.1.298)还原为3-HP。MCR是一种NADPH依赖性酶，其被一些嗜热嗜酸性古细菌用于经由3-羟基丙酸/4-羟基丁酸循环自养地将碳固定到有机物质中(Berg等人，Science，318:1782-1786,2007)。编码具有这种MCR活性的酶的基因被表征存在于勤奋金属球菌(Metallosphaera sedula)(GenBank No.：ABP94884.1，查询序列)和头寇岱硫化叶菌(GenBank No.：BAB67276.1，查询序列)中。虽然这些MCR都具有与橙色绿屈挠菌双功能丙二酰辅酶A还原酶类似的醛脱氢酶活性，但它们没有表现出任何显著的序列相似性，表明绿屈挠菌属和硫化叶菌科中的自养途径趋同进化，并且募集不同的基因以在这些分类群中执行相似的代谢过程(Alber等人，J.Bacteriol.188:8551-8559,2006)。特别地，古细菌MCR显示出与天冬氨酸-半醛脱氢酶的高的序列相似性。基于生物信息学分析和序列同源性推断具有该MCR活性的其他基因。各种基因总结在表6中。
2-5：3-羟基丙酸脱氢酶
丙二酸半醛可通过可逆的3-羟基丙酸脱氢酶(HPDH；EC 1.1.1.59，NADH依赖性的)或丙二酸半醛还原酶(EC 1.1.1.298，NADPH依赖性的)还原为3-HP。这些酶天然参与细菌和植物中的β-丙氨酸代谢、丙酸代谢或尿嘧啶降解。此外，一些嗜热嗜酸性古细菌需要这些酶以通过3-羟基丙酸/4-羟基丁酸循环固定碳(Kockelkorn和Fuchs，J.Bacteriol.191:6352-6362,2009)。已证明或假定在大肠杆菌(GenBank No.：EFV00080.1，查询序列)、酿酒酵母(GenBank No.：DAA10125.1，查询序列)、勤奋金属球菌(GenBank No.：ABP96133.1，查询序列)、头寇岱硫化叶菌(GenBank No.：BAK54608.1，查询序列)和大肠杆菌(GenBank No.：ACR64730.1，查询序列)中存在编码具有3-羟基丙酸脱氢酶或丙二酸半醛还原酶活性的酶的基因。基于生物信息学分析和序列同源性推断具有3-羟基丙酸脱氢酶或丙二酸半醛还原酶活性的其它基因。各种基因总结在表7中。
2-6：3-羟基异丁酸脱氢酶
3-羟基异丁酸脱氢酶(HIBADH；EC 1.1.1.31)是参与缬氨酸和其它支链氨基酸的代谢的关键酶。HIBADH催化3-羟基异丁酸向甲基丙二酸半醛的NADH依赖性或NADPH依赖性可逆转化。然而，由于其广泛的底物特异性，还已表明HIBADH通过将丙二酸半醛转化为3-HP显示出3-羟基丙酸脱氢酶活性(即EC 1.1.1.59)(Yao等人，Appl.Bio.Biotechnol.160:694-703,2010)。在恶臭假单胞菌(GenBank No.：ADR61938.1，查询序列)、绿脓杆菌(GenBank No.：AAG06957.1，查询序列)、蜡样芽胞杆菌(GenBank No.：AAP10961.1，查询序列)和粪产碱杆菌(GenBank No.：EJC65559.1，查询序列)中鉴定了具有HIBADH活性的酶。基于生物信息学分析和序列同源性推断具有该HIBADH活性的其他基因。各种基因总结在表8中。
2-7：4-羟基丁酸脱氢酶
4-羟基丁酸脱氢酶(HBDH；EC 1.1.1.61)是天然参与丁酸代谢的酶。HBDH催化4-羟基丁酸向琥珀酸半醛的可逆NAD+依赖性转化。然而，因为酶反应相似，HBDH也可以将丙二酸半醛转化为3-HP。已经在钩虫贪铜菌(GenBank No.：AAC41425.1，查询序列)和克氏梭菌(GenBank No.：EDK35022.1，查询序列)中鉴定了具有HBDH活性的酶。基于生物信息学分析和序列同源性推断具有该HBDH活性的其它基因。各种基因总结在表9中。
2-8：3-羟基丁酸脱氢酶
3-羟基丁酸脱氢酶(BDH；EC 1.1.1.30)是天然参与丁酸代谢的酶。BDH催化3-羟基丁酸向乙酰乙酸酯的可逆NAD+依赖性转化，但其也可氧化其它3-羟基一元羧酸。例如，因为酶反应是类似的，BDH可以将丙二酸半醛转化为3-HP。已经在绿脓杆菌(GenBank No.：GAA17557.1，查询序列)中鉴定了具有BDH活性的酶。基于生物信息学分析和序列同源性推断具有该BDH活性的其他基因。各种基因总结在表10中。
实施例3酶活性的测量
ACC分光光度酶测定
分光光度ACC测定是偶联测定，其中由ACC反应产生的产物在需要辅因子NAD(P)H的反应中被进一步消耗掉，可以用分光光度计监测辅因子NAD(P)H的氧化。
Kroeger等人(2011，Anal.Biochem.411：100-105)描述了偶联测定，其中由ACC1产生的丙二酰辅酶A通过纯化的丙二酰辅酶A还原酶(MCR)在需要NADPH作为辅因子的反应中进一步转化为丙二酸半醛。ACC活性通过以下NADPH氧化来测量。
Diacovich等人(2002，J.Biol.Chem.277:31228-31236)将ADP转化(在ACC反应中用作辅因子的ATP的水解产物)与需要ADP的丙酮酸激酶反应结合，该丙酮酸激酶反应进一步与使用乳酸脱氢酶形成丙酮酸的反应偶联。后一种酶需要NADH作为辅因子，随后该辅因子被氧化。
ACC放射性酶测定
最常用的体外ACC测定是基于放射性14C碳酸盐的使用。随后是将放射性碳酸盐掺入酸和非挥发性物质(即丙二酰辅酶A)中。通过酸和热处理将未转化为丙二酰辅酶A的14C标记的碳酸氢钠除去，酸和热处理将剩余的NaH14CO3和可能的反应副产物转化为14C标记的CO2。
经过略微的改变，已将由Diacovich等人(2002,J.Biol.Chem.277:31228-31236)描述的测定用于检测酵母溶解产物的ACC活性(Shi等人2014,mBIO 5:3e01130-14)。由在指数期晚期或稳定期收获的酵母细胞制备细胞溶解产物。洗涤细胞，然后重悬于含有100mMpH 7.5的磷酸钾、2mM MgCl2、1mM二硫苏糖醇和1×EDTA游离的完整蛋白酶抑制剂(Roche)的裂解缓冲液中。通过玻璃珠破碎细胞，并在4℃下离心后收集上清液。
ACC酶测定反应混合物包括100mM磷酸钾(pH 8.0)、300μg BSA、3mM ATP、5mMMgCl2、10mM NaH4CO3[比活性200μCi mmol-1(7400kBq mmol)]和0.5mM乙酰辅酶A。该反应的总体积为100μL，包括20μL的细胞提取物。
将反应在30℃下孵育15分钟，并通过加入50μL的5M HCl终止反应。将管内容物在95℃下蒸发至干，使残余物重悬于100μL水中，并与3mL闪烁混合液(scintillationcocktail)(Ultima Gold AB，PerkinElmer)混合。使用液体闪烁计数器(PerkinElmer Tri-Carb 2810TR)测定样品的14C含量。
AADH酶测定
按照Kozak等人(2014,Metab.Eng.21:46-59)的描述通过在30℃下在340nm处监测NAD+的还原来测量AADH活性。收集用于获得细胞溶解产物的酵母细胞，用水洗涤，然后重悬于含有100mM Tris-HCl缓冲液(pH 7.5)和1×EDTA游离蛋白酶抑制剂混合物(Roche)的裂解缓冲液中。将细胞在Precellys 24均质器中以5500rpm的速度用玻璃珠破碎3×40秒，在各轮破碎之间将细胞保持在冰上。将溶解产物于4℃下以16000g离心20分钟，收集上清液。使用Bradford方法测定总蛋白浓度。
酶测定反应混合物在200μL的总反应体积中含有0.1mM辅酶A、50mM CHES缓冲液(pH 9.5)、0.8mM NAD+、0.2mM DTT和10μL细胞提取物。通过加入10mM新制备的乙醛溶液开始反应，随后使用Thermo Konelab 20XT分析仪跟踪NAD+的还原。
酵母细胞溶解产物的MCR酶测定
根据经过略微改变的Chen等人(2014，Metab.Eng.22:104-109)描述的方法测量MCR酶活性。该方法以在340nm处监测NAD(P)H的氧化为基础。
收集细胞并用含有20mM Tris-HCl(pH 7.5)、20mM NaN3的冷的洗涤缓冲液洗涤，然后重悬浮于含有50mM HEPES(pH 7.5)、150mM KCl、1mM DTT、1mM EDTA、0.5％Triton X-100和1×EDTA游离蛋白酶抑制剂混合物(Roche)的1mL破碎缓冲液。将细胞在Precellys 24均质器中以5500rpm的速度用玻璃珠破碎3×40秒，在各轮破碎之间将细胞保持在冰上。将溶解产物在4℃下以16000g离心20分钟，收集上清液。使用Bradford方法测定总蛋白浓度。
MCR测定混合物含有50mM Tris-HCl缓冲液(pH 8.0)、5mM MgCl2和0.3mM NADPH或NADH。在将20μL细胞溶解产物加入到200μL的总反应体积中后，将反应在30℃下预孵育5分钟，之后通过加入0.15mM丙二酰辅酶A开始反应。使用Thermo Konelab 20XT分析仪在340nm监测测定。
酵母表达载体的产生
产生一系列酵母表达质粒以评估候选基因在酵母中的表达和活性能力。首先，设计了新的基于pBlueScript的多克隆位点(MCS)，使得可以利用所有可能的限制酶(RE)位点组合。然后将该修饰的MCS置于基于pRS的系列酵母着丝粒和多拷贝质粒中。之后，使用一组10个独特的RE位点，将九个不同组的启动子和终止子克隆到这些基于pRS的酵母表达载体中。因此，可以利用该质粒系统在合适的酵母菌株中要么以低拷贝数要么以高拷贝数同时组成型表达多达9个基因，以评价用于产生3-HP的多种途径酶组合。
ACC基因的克隆
已经转化到具有和不具有SNF1缺失的工业酿酒酵母VSK-128菌株的ACC1基因示于表13中。ACC基因从多拷贝质粒表达，其中它们在PDC1启动子的控制下。在Shi等人的出版物(2014)中描述的突变ACC1sc基因是用QuikChange II定点诱变试剂盒(安捷伦科技有限公司)构建的。
表13
转化到酿酒酵母VSK-128的乙酰辅酶A羧化酶基因
ACC酶测定
在文献中，分光光度测定法已经用于用纯化的或部分纯化的ACC酶检测ACC酶活性。用分光光度测定测试其中ACC过表达的酵母细胞溶解产物，但是与对照相比没有检测到吸光度变化。
放射性ACC酶测定是非常敏感的，甚至可以检测pmol/mg/min活性。首先用市售的纯化的人ACC酶测试该方法。当检测到明显的活性时，进一步优化该方法以检测酵母细胞溶解产物的ACC活性。测定表13中列出的菌株的ACC1活性，并基于该结果制作描述ACC1的相对排名的列表(图2)。对最有前途的候选者ACCyl进行更多的研究，酿酒酵母S-128野生型菌株的结果和其中ACC1已被过表达的相同菌株的结果显示在表14中。
表14
ACC1基因的过表达导致ACC1酶活性是野生型酿酒酵母VSK-128菌株的内源ACC1活性的2.9倍。
AADH体外酶活性测定
最初选择了5种AADH基因(即ADHEpm、ADHEec、EUTec、EUTdz和LIN1129li)，并转化到CEN.PK实验室菌株中。这些AADH从多拷贝质粒表达，其中它们在TEF1启动子的控制下。所有五个AADH基因显示AADH活性，但是与adhE型AADH(即ADHEpm和ADHEec)相比，三种eutE型AADH基因(即EUTec、EUTdz和LIN1129li)在酵母中给出高得多的AADH活性。
从基因组挖掘分析中选择20个其他新AADH基因以评估酵母中的表达和酶活性。测试这二十个新的AADH基因的体外酶活性，其中四个(即AADHmm、AADHab、AADHbw和AADHvs)显示具有AADH活性。
AADH体内生长测定
从工业酿酒酵母VSK-128菌株中删除所有ACS2基因拷贝以产生具有缺陷型PDH旁路的菌株，该菌株在基于葡萄糖的培养基上不能生长。然后将携带25种不同AADH变体的表达载体转化到该ACS2缺失菌株中，以评价它们恢复该菌株在葡萄糖上的生长的能力。
基于菌株在葡萄糖基琼脂平板上生长的能力，12种不同的AADH变体(即EUTEec、EUTEdz、LIN1129li、AADHmm、AADHt1、AADHab、AADHta、AADHbs、AADHbw、AADHvs、AADHhs和ADHEec)能够恢复ACS2-缺失菌株的生长。体外AADH酶活性测定和体内生长恢复分析之间存在良好的相关性。
然后在液体摇瓶培养中评估被发现能够恢复ACS2缺失菌株生长的前九个AADH变体菌株的生长速率，并与含有完整PDH旁路的野生型菌株进行比较。当在葡萄糖上生长时，所有9个这些AADH变体都能够维持>50％的酿酒酵母VSK-128菌株有氧生长速率，并且这些AADH变体中的6个能够维持≥80％的酿酒酵母VSK-128菌株有氧生长速率。
酵母中的MCR酶测定
将八个全长绿屈挠菌属MCR同源物截短成它们的两个功能结构域，并且测定MCR特异性结构域以及六个古细菌MCR在酵母中的酶活性。这些MCR从多拷贝质粒表达，其中它们在TEF1启动子的控制下并转化到CEN.PK实验室菌株中。
当利用NADPH作为辅因子时，MCRca(橙色绿屈挠菌)及其同源物MCRrc(光合玫瑰弯菌)是仅有的两种给出比野生型菌株更高的MCR酶活性的绿屈挠菌属MCR同源物。当利用NADH作为辅因子时，没有观察到这八种绿屈挠菌属MCR同源物的酶活性。
当利用NADPH作为辅因子时，三个古细菌MCR[Sulfolobales archaeon、嗜酸热硫化叶菌(x2)]给出了比野生型菌株高的MCR酶活性，使用NADH作为辅因子时，所有六个古细菌MCR都给出比野生型菌株高的MCR酶活性。
古细菌MCR的异源表达和表征
为四种不同的古细菌MCR(勤奋金属球菌(MCRms)、头寇岱硫化叶菌(MCRst)，Candidatus caldiarchaeum(MCRcc)和古菌硫化叶菌(MCRsa1))制备基于pBAT T7启动子的表达构建体。这些构建体不含有纯化标签，是大肠杆菌密码子优化的且在本报告前面所述的条件下表达(表15)。
表15
用于在大肠杆菌中表达的古细菌MCR构建体的概述
(ca-橙色绿屈挠菌，ms-勤奋金属球菌，st-头寇岱硫化叶菌，cc-Candidatuscaldiarchaeum，sa1-古菌硫化叶菌，sa2-嗜酸热硫化叶菌)
使用以下测定条件分析它们的活性：0.4mM NAD(P)H，0.15mM丙二酰辅酶A，Tris-HCl pH 7，2mM MgCl2。在室温下以微量滴定板(MTP)形式对20倍稀释的溶解产物进行测定。随后及时追踪A365处的NADPH或NADH氧化。在测试的四个古细菌基因中，MCRsa1和MCRst显示最高的MCR活性。然而，MCR活性小于测量的标记的MCRca的活性。对于Candidatuscaldiarchaeum MCR(MCRcc)，没有测量到对NADPH或NADH的活性(在大肠杆菌细胞溶解产物中)。
当使用SDS-PAGE凝胶分析这些构建体时，MCRsa1在大肠杆菌溶解产物中显示最高的表达水平，而MCRcc不能以可溶形式表达；参见凝胶(sa1>ca>st>ms>cc)(图3)。构建体MCRsa1和MCRst似乎具有双重辅因子偏好，并且与测量到的对NADPH的活性相比，显示出约40-50％的相对NADH活性。就比活性而言，MCRst可以是所测试的四种古细菌MCR中活性最高的酶，因为它在相对低的表达水平下显示相对高的活性水平。
实施例4通过培养重组酵母产生3-HP
酿酒酵母的摇瓶培养
从在选择性琼脂基平板上新生长的菌株中取出一小圈细胞，用于接种在250mL烧瓶中的20mL选择性SC基培养基(20g/L葡萄糖)，并生长2天(30℃，250rpm)，直到所有葡萄糖和乙醇已被消耗。测定最终细胞密度，将培养物在4000rpm离心5分钟。然后通过HPLC或GC/MS分析上清液以确定培养物上清液中3-HP和其它主要代谢物的积累。然而，还根据具体菌株和目的来测试其他培养条件(例如葡萄糖的起始量、通气量、培养基的类型和向培养基中加入另外的物质等)。
实施例5酿酒酵母的生物反应器培养
在含有250-500mL培养基的Multifors生物反应器(最大工作体积500mL，24叶片Rusthon涡轮叶轮，Infors HT，瑞士)中进行培养。最初将培养物维持在30℃，300或900-950rpm，1.2、2.4或3.6体积气体(体积培养)-1min-1(vvm)。通过加入无菌2M NaOH或2M H3PO4使培养物pH在pH 5.5±0.2处恒定。加入Clerol FBA 3107消泡剂(Cognis France，Ponthierry Paris；0.03％v/v)以控制泡沫产生。在用含3％CO2的Ar；含5％CO2、0.99％Ar、15％O2的N2；含20％O2加20％Ar的N2；含0.04％乙醇的N2校准的Prima Pro Process质谱仪(Thermo Scientific，英国)中连续分析气体浓度(CO2、O2、N2和Ar)。
将菌株在摇动的烧瓶中在SCD基选择性培养基中预生长过夜，并用于接种生物反应器。使分批培养阶段(20g/L初始葡萄糖)持续14至20小时，并且仅在葡萄糖已被消耗后开始葡萄糖进料，但葡萄糖进料在乙醇已被消耗之前或之后开始(取决于培养目的)。葡萄糖进料速率保持在0.38-0.65g L-1h-1(取决于培养目的)。然后通过HPLC分析上清液样品，以确定培养物中3-HP和其他主要代谢物的积累。
实施例6通过HPLC对细胞培养物上清液进行3-HP分析
用Waters Alliance e2695HPLC系统(Waters，美国米尔福德)分析培养物上清液样品，其中注射体积为10μl。使用与Fast Acid Analysis Column(100mm×7.8mm)(Bio-Rad，美国)连接的Aminex HPX-87H Organic Acid Column(300mm×7.8mm)(Bio-Rad，美国)作为HPLC的固定相。将柱保持在+55℃，并使用5.0mM H2SO4(Merck KgaA，德国)作为洗脱液，流速为0.3ml min-1或0.5ml min-1。使用Waters 2489双波长UV(210nm)检测器(Waters，美国米尔福德)和Waters 2414差示折射计(Waters，美国米尔福德)检测3-羟基丙酸、葡萄糖、乙酸、琥珀酸、丙酮酸、甘油和乙醇。
实施例7通过GC/MS对细胞培养物上清液进行3-HP分析
按以下方式制备测试样品并制作标准曲线：用50μl HCl(6N)酸化上清液(0.5ml)，并掺加3-HPA(TCI)标准品(在乙酸乙酯中)。加入5μl的乳酸内标溶液(Sigma Aldrich(ISOTEC)L-乳酸钠-3,3,3-d3 98原子％；5.5g/l)和约0.2g的NaCl。由于标记的3-HP不可商购获得，因此选择该乳酸稳定同位素产品作为内标，因为该产品是可获得的、在样品基质中不存在、与3-HP在结构/化学方面最相似的化合物。将混合物在涡旋混合器中振荡约3分钟。然后采用0.5ml乙酸乙酯通过在涡旋混合器中混合约3分钟萃取样品两次。通过在10000rpm离心5分钟分离各层。将上层收集到GC小瓶中并蒸发。通过在60℃下孵育1小时，用含有1％TMCS的MSTFA(50μl)将干燥的残余物衍生化。以与样品相同的方式提取校准曲线的标准品以使误差最小化。
在与Agilent 5973质量选择性检测器(MSD)结合的Agilent 6890气相色谱仪(GC)上运行样品。注射器(注射体积1μl，分流比20:1)和MSD界面温度为280℃，并且炉温程序为以20℃/min的速率从50℃升至280℃。在Agilent HP-5MS毛细管柱(30m，ID 200μm，膜厚度0.25μm；Agilent 19091S-433)上进行分析。化合物的鉴定基于对NIST文库的光谱搜索。通过监测m/z 147和m/z219检测3-HP，通过监测m/z 177检测3-HP二聚体。通过利用3-HP响应(responses)构建5点校准曲线(c＝1-400mg/l)，并使用内标归一化。证明在该浓度范围内的定量是线性的。空白样品与样品一起分析。
实施例8酿酒酵母质粒表达菌株的评价
3-HP质粒表达菌株同时从两种不同的表达质粒(即pSK-084和/或pSK-085)表达一种、两种或三种3-HP途径酶(即AADH、ACC1、MCR和HPDH)(图4)。这些菌株用于评价不同的3-HP途径酶(和这些酶的组合)对VSK-128耐酸酿酒酵母菌株中3-HP的产量的影响。将菌株在250mL烧瓶中的20mL选择性SC基培养基(20g/L葡萄糖)中培养，并生长2天(30℃，250rpm)直到所有葡萄糖和乙醇被消耗掉。
实施例9体内途径酶活性分析的总结
对表16中的25个AADH、8个ACC1、10个双功能HPDH-MCR、6个古细菌MCR和28个HPDH在各种酿酒酵母菌株中产生3-HP的能力进行分析，如果需要，所述酿酒酵母菌株也表达额外的3-HP途径酶。显示许多新的3-HP途径酶(从基因组挖掘分析获得)在酵母中是有活性的，并且与先前公开的3-HP途径酶相比，它们中的许多具有更优异的性质(即更高的活性，更好的辅因子偏好)。
表16
实施例10用于3-HP生产的酿酒酵母摇瓶培养试验
培养条件
在一对早期测试菌株上评价各种不同的培养条件，以观察各种培养条件如何影响菌株产生3-HP的能力。酿酒酵母VSK-128(Δura3，Δhis3)菌株表达两种质粒，其中一种质粒含有HIBADHpa或HPDHec基因，第二种质粒含有MCRsa2基因。两种菌株在培养期间表现相似，并且具有非常相似的代谢物谱图。
测试六种不同的摇瓶培养条件：
1.有氧，分批法，高初始葡萄糖(120g/L)。在第3天再加入100g/L葡萄糖。
2.无氧，分批法，高初始葡萄糖(100g/L)。将烧瓶密封并且摇动更慢，速度为100rpm。
3.有氧，分批法，重复掺加葡萄糖(glucose spiking)。初始葡萄糖为20g/L，然后每隔一天加入40g/L。
4.有氧，模拟分批补料法，许多初始葡萄糖片。最初加入5片，在第3天再加入5片。每天加入不同量的酶A溶液(50-150μl/天)。
5.有氧，模拟分批补料法，重复掺加葡萄糖片。最初加入1片，在第1天和第2天加入2片，在第3天和第4天加入3片。每天加入不同量的酶A溶液(50-150μl/天)。
6.好氧，分批法，重复掺加半乳糖。初始半乳糖为20g/L，然后每隔一天加入40g/L。
对于模拟补料分批培养，每个片剂被认为释放0.5g葡萄糖(对于25-mL培养体积而言，其相当于20g/L葡萄糖)。片剂基本上由葡萄糖(即淀粉)组成，酶A溶液(即淀粉酶)允许在摇瓶培养期间将葡萄糖受控地缓慢释放到培养基中。假设葡萄糖受限的补料分批条件可以促进生长和随后的3-HP生产的效率(flux)。
细胞生长
所有正常的基于葡萄糖的培养均类似地生长，并且半乳糖补料培养生长更慢。另一方面，与其他培养条件相比，分批补料条件促进更多的细胞生长。特别地，分批补料(片剂掺加)条件在培养早期的确促进了大量生长。
3-HP生产
对于这些培养，大多数生长通常在第2天结束时发生，并且绝大多数3-HP也在第2天生成，因此表明生长和3-HP产生彼此相关。分批补料(片剂掺加)培养条件产生最多的3-HP(～0.85g/L)，半乳糖进料培养产生最少量的3-HP(～0.12g/L)，其他培养条件全部产生约0.3-0.4g/L的3-HP。这些结果表明培养条件可对3-HP产生水平有大的影响(图5)。
葡萄糖消耗
在所有培养试验中葡萄糖被快速消耗直到第2-3天，然后葡萄糖消耗速率随着生长和3-HP产生显著降低(在第4-5天期间)。对于补料分批培养，似乎葡萄糖的消耗与其从片剂释放一样快，表明这些培养在葡萄糖受限的条件下进行。
甘油、乙酸和乙醇积累
对于葡萄糖进料培养，甘油积累是相当高的，对于半乳糖进料培养而言甘油积累则低得多。乙酸积累在不同的培养条件中相当相似，但是补料分批(许多片剂)条件产生更多的乙酸。
对于这些摇瓶培养中的大部分，特别是对于葡萄糖分批培养和葡萄糖掺加培养，存在大量的乙醇累积。补料分批培养旨在代表葡萄糖受限条件以减少过量溢流代谢生成乙醇，并且这种方法似乎已经取得成功，因为在补料分批培养中乙醇积累显著较低。
培养更成熟的3-HP产生菌株
按照最有前途的补料分批(片剂掺加)培养条件培养其他3-HP生产菌株[(EutEec、HPDH-MCRca、ACC1sc_S1157A)和(HIBADHpa、MCRsa2)]以检查其性能。
同样，大部分生长发生在第3天，大部分3-HP产生发生在第3天。在该培养条件下，该菌株的3-HP产量超过1.2g/L(图6)。
尽管详细描述了本发明的具体实施方案，但是对于本领域技术人员来说显而易见的是，具体描述仅仅是令人满意的示例性实施方案，并且不应被解释为限制本发明的范围。因此，本发明的实质范围由所附权利要求及其等同物限定。
工业适用性
使用本发明的重组酵母和制备3-HP的方法使得能够以高浓度和高收率在低pH下从有用的糖(如葡萄糖)生产3-HP，从而极大地有助于从生物质经济地生产3-HP及其应用产品。
序列表
<110> SK新技术株式会社
<120> 产生3-羟基丙酸的重组酵母和使用其产生3-羟基丙酸的方法
<130> PF-B1841
<140> PCT/KR2015/009061
<141> 2015-08-28
<150> 10-2014-0114505
<151> 2014-08-29
<160> 144
<170> PatentIn version 3.2
<210> 1
<211> 2248
<212> PRT
<213> Kazachstania exigua
<400> 1
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Ala Leu His Ser Arg Leu Pro Met Ser Leu Asp Glu Lys Leu Asp Gln
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Val Ile Thr Glu Arg Val Val Glu Glu Gly Glu Glu Arg Tyr Val
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Gln Asp Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly
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Glu Gly Gln Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys
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Val Leu Gly Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly
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Thr Gln Ile Met Tyr Asn Asn Gly Val Ser His Leu Thr Ala Ser
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Asp Asp Leu Gln Ala Val Glu Gln Ile Ile Asn Trp Leu Ser Tyr
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Val Pro Ala Lys Arg Asn Met Pro Val Pro Ile Leu Glu Thr Glu
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Thr Leu Ser Gly Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg
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Leu Gly Gly Ile Pro Ile Gly Val Ile Gly Val Glu Thr Arg Thr
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Ile Glu Asn Val Ile Pro Ala Asp Pro Ala Asn Pro Asp Ser Thr
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Glu Thr Val Val Gln Glu Ala Gly Gln Val Trp Tyr Pro Asn Ser
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Ala Phe Lys Thr Ala Gln Ala Ile Gln Asp Phe Asn His Gly Glu
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Gln Leu Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly
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Gly Gln Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe
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Ile Val Asp Ala Leu Val Asp Tyr Lys Gln Pro Ile Phe Ile Tyr
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2015 2020 2025
Asp Ser Arg Ala Gly Val Leu Glu Pro Ser Gly Met Val Gly Ile
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Lys Tyr Arg Arg Glu Lys Leu Leu Ser Thr Ile Thr Arg Leu Asp
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Ser Thr Tyr Arg Glu Leu Lys Glu Lys Leu Ser Asp Lys Leu Leu
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Ser Thr Glu Glu His Gln Lys Ile Ser Ile Gln Leu Ala Glu Arg
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Glu Lys Met Leu Ile Pro Ile Tyr Asn Gln Ile Ser Val Gln Phe
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Ala Asp Leu His Asp Arg Ser Ser Arg Met Leu Lys Lys Gly Val
2105 2110 2115
Ile Lys Ala Glu Leu Thr Trp Glu Glu Ser Arg Arg Tyr Phe Phe
2120 2125 2130
Trp Arg Leu Arg Arg Arg Leu Asp Glu Glu Tyr Leu Leu Arg Arg
2135 2140 2145
Leu Asp Ser Gln Leu Thr Gln Ala Ser Arg Leu Glu Lys Ile Ala
2150 2155 2160
Arg Val Arg Ser Trp Tyr Pro Thr Ser Val Asp Val Glu Asn Asp
2165 2170 2175
Lys Glu Val Val Thr Trp Ile Glu Glu Asn Tyr Glu Thr Leu Glu
2180 2185 2190
Glu Lys Phe Lys Ala Leu Lys Leu Glu Ser Phe Ala Leu Asp Leu
2195 2200 2205
Ala Lys Asn Ile Arg Asn Asp His Glu Asn Ser Met Lys Gly Leu
2210 2215 2220
Glu Glu Val Val Arg Met Leu Ser Ala Thr Asp Lys Glu Arg Leu
2225 2230 2235
Asn Lys Ile Ile Asn Pro Asp Asp Lys Lys
2240 2245
<210> 2
<211> 2243
<212> PRT
<213> 小假丝酵母
<400> 2
Met Ser Glu Glu Asn Leu Phe Glu Asp Ala Ala Ala Lys Arg Gln Tyr
1 5 10 15
Glu Ile Thr Asp Tyr Ser Thr Lys His Lys Thr Leu Ala Ser His Phe
20 25 30
Val Gly Leu Asn Thr Leu Glu Arg Val Glu Glu Ser Pro Leu Lys Asp
35 40 45
Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile
50 55 60
Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys
65 70 75 80
Trp Ala Tyr Glu Thr Phe Gly Asp Glu Arg Thr Val Gln Phe Val Ala
85 90 95
Met Ala Thr Pro Glu Asp Leu Gln Ala Asn Ser Glu Tyr Ile Arg Met
100 105 110
Ala Asp Gln Tyr Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr
115 120 125
Ala Asn Val Asp Leu Ile Val Asp Val Ala Glu Arg Thr Asp Val Asp
130 135 140
Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro His Leu Pro
145 150 155 160
Glu Lys Leu Ala Ala Ser Lys Arg Lys Ile Ile Phe Ile Gly Pro Pro
165 170 175
Gly Ser Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val
180 185 190
Ala Gln His Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val
195 200 205
Asp Thr Val His Leu Asp Ser Glu Thr Gly Leu Val Ser Val Asp Asp
210 215 220
Asp Ile Tyr Gln Lys Gly Cys Cys Ser Ser Pro Glu Asp Gly Leu Ala
225 230 235 240
Lys Ala Lys Lys Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly
245 250 255
Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile
260 265 270
Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe
275 280 285
Ile Met Lys Leu Ala Gly Lys Ala Arg His Leu Glu Val Gln Leu Leu
290 295 300
Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser
305 310 315 320
Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile
325 330 335
Ala Ser Asn Glu Thr Phe Glu Lys Met Ala Ser Ala Ala Val Arg Leu
340 345 350
Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr
355 360 365
Ser Ser Glu Glu Lys Lys Tyr Tyr Phe Leu Glu Leu Asn Pro Arg Leu
370 375 380
Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro
385 390 395 400
Ser Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser
405 410 415
Asp Ile Arg Val Leu Tyr Gly Met Asn Pro His Gly Ala Thr Glu Ile
420 425 430
Asp Phe Glu Phe Lys Thr Glu Glu Ala Lys Lys Thr Gln Arg Lys Pro
435 440 445
Val Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro
450 455 460
Asn Glu Gly Phe Lys Pro Ser Gly Gly Ser Leu His Glu Leu Asn Phe
465 470 475 480
Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly
485 490 495
Gly Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe
500 505 510
Gly Glu Asn Arg Gln Met Ser Arg Lys His Met Val Val Ala Leu Lys
515 520 525
Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile
530 535 540
Lys Leu Leu Glu Thr Glu Asp Phe Glu Glu Asn Thr Ile Thr Thr Gly
545 550 555 560
Trp Leu Asp Asp Leu Ile Ser His Lys Met Thr Ala Gln Lys Pro Asp
565 570 575
Ala Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Ile Ala
580 585 590
Ser Glu Ser Ala Arg Lys Glu Tyr Ile Asn Gln Leu Lys Arg Gly Gln
595 600 605
Val Pro Pro Lys Gly Leu Leu Glu Thr Met Phe Pro Ile Glu Phe Ile
610 615 620
His Glu Asn Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Ala Asp Asp
625 630 635 640
Ser Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Glu Val Arg Ala Val
645 650 655
Lys Leu Ser Asp Gly Gly Leu Leu Ile Ser Val Gly Gly Gln Ser His
660 665 670
Thr Ile Tyr Trp Lys Glu Glu Ala Gln Ala Thr Arg Leu Ser Ile Asp
675 680 685
Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg
690 695 700
Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Asp
705 710 715 720
Gln Ile Lys Val Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met
725 730 735
Gln Met Pro Leu Val Ser Gln Glu Asn Gly Val Ile Gln Leu Leu Lys
740 745 750
Gln Pro Gly Ser Thr Ile Asp Ala Gly Asp Ile Ile Ala Met Leu Ala
755 760 765
Leu Asp Asp Pro Ser Lys Val Lys His Ala Met Pro Phe Glu Gly Met
770 775 780
Leu Pro Glu Leu Gly Ser Pro Met Val Glu Gly Thr Lys Pro Ala Tyr
785 790 795 800
Lys Phe His Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr
805 810 815
Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val
820 825 830
Leu Arg Asp Pro Arg Leu Pro Tyr Ser Glu Trp Lys Leu Asn Val Ser
835 840 845
Ala Leu His Thr Arg Leu Pro Leu Ala Leu Asp Glu Lys Leu Asp Lys
850 855 860
Leu Val Glu Arg Ser Ala Asn Arg Ser Ala Ile Phe Pro Ala Lys Gln
865 870 875 880
Leu Gln Lys Met Ile Asp Asp Ala Val Arg Asp Pro Gln Ala Asp Pro
885 890 895
Leu Leu Ser Ser Ile Val Glu Pro Leu Leu Asp Ile Thr Thr Arg Tyr
900 905 910
Ala Asn Gly Leu Glu Ala His Glu His Ser Val Phe Val Lys Phe Leu
915 920 925
Glu Glu Tyr Tyr Asn Val Glu Lys Leu Phe Ser Glu His Gly Glu Arg
930 935 940
Ala Glu Asn Val Ile Leu Lys Leu Arg Asp Glu Asn Ile Asp Asp Leu
945 950 955 960
Asp Lys Val Val Leu Ile Val Leu Ser His Ala Lys Val Ser Ala Lys
965 970 975
Asn Thr Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Ile Cys Lys
980 985 990
Met Ser Ser Ser Val Ala His Ile Phe Thr Lys Pro Leu Gln His Ile
995 1000 1005
Val Glu Leu Glu Ser Lys Pro Ala Ala Lys Val Ala Leu Gln Ala
1010 1015 1020
Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg
1025 1030 1035
Thr Glu Gln Ile Glu His Ile Leu Arg Ser Ser Val Thr Asn Thr
1040 1045 1050
Ser Tyr Gly Asp Ser Ser Pro Lys Val Ser Glu Pro Asn Met Glu
1055 1060 1065
Ile Leu Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val
1070 1075 1080
Leu Leu Pro Phe Leu Cys His Glu Asp Pro Val Ile Ala Thr Ala
1085 1090 1095
Ala Gly Gln Val Tyr Val Arg Arg Ala Tyr Arg Ala Tyr Thr Val
1100 1105 1110
Gly Glu Val Lys Ser His Ser Ile Thr Asp Ser Ser Met Asn Pro
1115 1120 1125
Leu Ile Glu Trp Lys Phe Gln Leu Pro Ser Ala Ala Phe Ser Ser
1130 1135 1140
Ile Pro Gln Leu Lys Ser Lys Ile Gly Met Asn Arg Ala Met Ser
1145 1150 1155
Val Ser Asp Leu Ser Tyr Val Val Asp Ser Glu His Ala Pro Leu
1160 1165 1170
Arg Thr Gly Ile Leu Val Ala Ala Glu His Leu Asp Glu Val Asp
1175 1180 1185
Ala Ser Leu Ala Gln Gly Leu Glu Val Ile Pro Glu His Ser Ala
1190 1195 1200
Pro Gly Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ser Ser
1205 1210 1215
Leu Ser Asn Ile Val Asn Val Cys Val Ala Ser Thr Glu Gly Phe
1220 1225 1230
Asp Ser Glu Lys Glu Ile Leu Ala Arg Val Asn Glu Ile Leu Glu
1235 1240 1245
Leu Asn Lys Ala Asp Leu Ile Lys Ser Ser Ile Arg Arg Ile Thr
1250 1255 1260
Phe Met Phe Gly Phe Pro Asp Gly Thr Tyr Pro Lys Tyr Tyr Thr
1265 1270 1275
Phe Asn Gly Pro Asn Tyr Gln Glu Asn Gln Thr Ile Arg His Ile
1280 1285 1290
Glu Pro Ala Cys Ala Phe Gln Leu Glu Leu Gly Arg Met Ser Asn
1295 1300 1305
Phe Asn Ile Thr Pro Ile Phe Thr Asp Asn Arg Asn Ile His Val
1310 1315 1320
Tyr Glu Ala Val Ser Lys Thr Ser Ala Leu Asp Lys Arg Phe Phe
1325 1330 1335
Thr Arg Gly Ile Ile Arg Thr Gly Arg Ile Arg Asp Glu Ile Ser
1340 1345 1350
Ile Gln Glu Tyr Leu Thr Ser Glu Ala Asn Lys Leu Met Ser Asp
1355 1360 1365
Ile Leu Asp Asn Leu Glu Ile Ile Asp Thr Ser Asn Ser Asp Leu
1370 1375 1380
Asn His Ile Phe Ile Asn Phe Ser Ala Val Phe Asp Val Ser Pro
1385 1390 1395
Glu Asp Val Glu Ala Ala Phe Gly Gly Phe Leu Glu Arg Phe Gly
1400 1405 1410
Lys Arg Phe Leu Arg Leu Arg Val Ser Ala Ala Glu Ile Arg Ile
1415 1420 1425
Ile Ile Gln Asp Pro Lys Thr Gly Ala Pro Val Pro Leu Arg Ala
1430 1435 1440
Leu Ile Asn Asn Val Ser Gly Tyr Val Val Lys Ser Glu Leu Tyr
1445 1450 1455
Thr Glu Ile Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu
1460 1465 1470
Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr
1475 1480 1485
Pro Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu
1490 1495 1500
Met Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe His Gln
1505 1510 1515
Ala Ala Ile Thr Thr Trp Gln Lys Tyr Lys Thr Asn Ser Ser Gln
1520 1525 1530
Lys Asp Leu Thr Thr Met Lys Glu Arg Asp Asp Leu Phe Ile Ser
1535 1540 1545
His Glu Leu Ile Glu Asp Glu Ser Gly Glu Leu Thr Glu Val Glu
1550 1555 1560
Arg Glu Pro Gly Ala Asn Ser Ile Gly Met Val Ala Phe Lys Val
1565 1570 1575
Thr Met Lys Thr Pro Glu Tyr Pro Arg Gly Arg Gln Phe Val Ile
1580 1585 1590
Val Ala Asn Asp Ile Thr Tyr Lys Ile Gly Ser Phe Gly Pro Gln
1595 1600 1605
Glu Asp Glu Phe Phe Asn Lys Val Thr Glu Tyr Ala Arg Lys Arg
1610 1615 1620
Gly Ile Pro Arg Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg Ile
1625 1630 1635
Gly Ile Ala Glu Glu Leu Val Pro Leu Tyr Gln Val Ala Trp Lys
1640 1645 1650
Asp Ala Asp Val Pro Ala Lys Gly Phe Glu Tyr Ile Tyr Leu Asn
1655 1660 1665
Lys Asp Glu Met Glu Thr Leu Lys Gly His Ser Lys Glu Asn Ser
1670 1675 1680
Val Ile Thr Glu Arg Ile Val Glu Asp Gly Glu Glu Arg Phe Val
1685 1690 1695
Ile Lys Ala Ile Ile Gly Ala Asp Asp Gly Leu Gly Val Glu Cys
1700 1705 1710
Leu Arg Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr
1715 1720 1725
Gln Asp Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly
1730 1735 1740
Ile Gly Ala Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Ile
1745 1750 1755
Glu Gly Gln Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys
1760 1765 1770
Val Leu Gly Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly
1775 1780 1785
Thr Gln Ile Met Tyr Asn Asn Gly Val Ser His Leu Thr Ala Ser
1790 1795 1800
Asp Asp Leu Gln Ala Val Glu Gln Ile Ile Lys Trp Leu Ser Tyr
1805 1810 1815
Val Pro Ala Lys Arg Asn Met Pro Val Pro Ile Leu Glu Asn Gly
1820 1825 1830
Asp Lys Trp Asp Arg Gln Ile Asp Tyr Arg Pro Thr Val Asn Glu
1835 1840 1845
Gln Tyr Asp Val Arg Trp Met Ile Glu Gly Arg Glu Leu Glu Asp
1850 1855 1860
Gly Arg Phe Glu Tyr Gly Met Phe Asp Lys Gly Ser Phe Phe Glu
1865 1870 1875
Thr Leu Ser Gly Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg
1880 1885 1890
Leu Gly Gly Ile Pro Ile Gly Val Ile Gly Val Glu Thr Arg Thr
1895 1900 1905
Ile Glu Asn Val Ile Pro Ala Asp Pro Ala Asn Pro Asp Ser Thr
1910 1915 1920
Glu Ala Val Val Gln Glu Ala Gly Gln Val Trp Tyr Pro Asn Ser
1925 1930 1935
Ala Phe Lys Thr Ala Gln Ala Ile Lys Asp Phe Asn His Gly Glu
1940 1945 1950
Gln Leu Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly
1955 1960 1965
Gly Gln Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe
1970 1975 1980
Ile Val Asp Ser Leu Val Asp Tyr Lys Gln Pro Ile Phe Ile Tyr
1985 1990 1995
Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp Val Val Val
2000 2005 2010
Asp Pro Thr Ile Asn Ala Asp Gln Met Glu Met Tyr Ala Asp Lys
2015 2020 2025
Asp Ser Arg Ala Gly Val Leu Glu Pro Ser Gly Met Val Gly Ile
2030 2035 2040
Lys Tyr Arg Arg Glu Lys Leu Leu Ala Thr Met Thr Arg Leu Asp
2045 2050 2055
Thr Thr Tyr Arg Glu Leu Lys Glu Gln Leu Ser Asp Lys Thr Leu
2060 2065 2070
Thr Pro Ala Gln His Gln Glu Val Ser Val Lys Leu Ala Glu Arg
2075 2080 2085
Glu Lys Phe Leu Leu Pro Ile Tyr Thr Gln Val Ser Val Gln Phe
2090 2095 2100
Ala Asp Leu His Asp Arg Ser Ser Arg Met Val Lys Lys Gly Val
2105 2110 2115
Ile Arg Ala Glu Leu Thr Trp Glu Glu Ser Arg Arg Phe Phe Phe
2120 2125 2130
Trp Arg Leu Arg Arg Lys Leu Asp Glu Glu Tyr Leu Val Lys Arg
2135 2140 2145
Leu Asp Ile Glu Leu Pro His Ala Ser Arg Leu Glu Lys Leu Ala
2150 2155 2160
Arg Leu Arg Ser Trp Tyr Pro Ala Ser Ile Asp Glu Thr Lys Asp
2165 2170 2175
Arg Asp Val Val Thr Trp Ile Glu Glu Asn Tyr Asn Val Phe Glu
2180 2185 2190
Glu Glu Phe Lys Ala Leu Lys Leu Glu Ser Phe Ala Gln Glu Leu
2195 2200 2205
Ala Lys Ser Met Arg Thr Asp His Asp Val Thr Met Arg Gly Leu
2210 2215 2220
Glu Glu Ile Ala Arg Met Leu Ser Lys Glu Asp Lys Glu Arg Leu
2225 2230 2235
Leu Ser Ala Leu Lys
2240
<210> 3
<211> 1443
<212> DNA
<213> AADHab
<400> 3
atggaaatta acgaaaaaga tttgaagttg attattgaaa acgtattgga caagttagaa 60
agtgacaaga gtgataaaaa gatattcgat tcttcattga atattccatc cagtggtcaa 120
gacggtatat ttgaagaagt caacgctgca gtagatgccg ctgaagcagc ccatttggaa 180
ttaatgaaat tgacattaga aaagagaaga gaaataatca gatctataag aaagatcata 240
atgtctaatt tggaagaaat ctcaaagtta gctgtcgaag aaactacatt cggtagagta 300
gaagataaga tcgaaaagaa cagattggct gcattaaaaa caccaggtat tgaagatttg 360
gaacctaccg catactcaga tgacaacggt atgactttga tggaaagagc cgcttacggt 420
gttataggtt ccataatccc aagtactaac cctacatctt cagttatcaa taacggtatt 480
tccatgatag ctggtggtaa tagtgttgtc tttaacccac atcctgcagc caaaagatcc 540
agttgtttca caatctcctt gattaatcaa gcaatagtta aggccggtgg tccacctaat 600
gtcttgtgcg ccattgctaa cccaactata gattctgcac aaacattgat gaagcaccca 660
aaaattaagt tgttagtagt taccggtggt cctgcagtcg taaagactgc tatgaactct 720
gataaaaagg ttattgctgc aggtccaggt aacccacctt gtgttgtcga tgaaactgct 780
gacttagtta aagcaggtag agatattgtc aatggtgctg gttttgacaa taacatagtt 840
tgtatctgcg aaaaggaaat attggccgtt tctcaaatcg ctgatcaatt gaaggaagaa 900
atgattaaga atggtgccca tgaattgaag ggtgaacaaa tcgaaaaggt tacaaagtta 960
gtcattaccg atccaggtaa acctggtcac gaaggtgcac caaataagaa atacgtcggt 1020
aaaaatgctt ctgtaattgc aagagatata ggtttagaca tctcagaaca aacaaagata 1080
ttgttgtgtg aagtagatag ataccatcct ttggtttgga ccgaacaatt gttaccagta 1140
attccattgg ttagattcaa taacgttgat gaagccatag actttgctgt tcaatgtgaa 1200
cataatttca gacactctgc ttcaatccac tctaaaaaca ttgcaacatt gtcaagaatg 1260
gccaagttga tgaactgctc cttattcatt aagaatggtc caaactacag tggtttaggt 1320
tttggtggtg ctggttacac ctcattcact atagcaaccc ctactggtga aggtttgact 1380
agagctagaa cttttactag agaaagaaga tgtgtattag ttggttactt cagaattgtt 1440
tga 1443
<210> 4
<211> 1434
<212> DNA
<213> AADHal
<400> 4
atggtagatc aaaccttaat agaacaaata acaagagccg tattgaccca aatgaaagct 60
ggtaaagacg ccgccgtatc cggtgacggt atttttgcca cagttgatca agctgtcgct 120
gcagccagac aagcttatca agaattgaga ttgttaacct tggaaaagag agaaactttg 180
ataagagcta tcagagatgc tgcattcgca aatgccgctg taattgccca aatggctgtt 240
caagaatccg gtatgggtag agttgaagat aagatcttga agaaccaatt agcagccaga 300
aaaaccccag gtactgaaga tttgacttct agagcttggt caggagatca tggtttgaca 360
ttagtagaaa tggcccctta cggtgttatt ggtgctataa caccaactac aaatccttct 420
gaaaccgtta tttgtaactc aatcggtatg attgctgcag gtaattccat agtttttagt 480
ccacacccta ctgctcaaaa tacatccttg accactatca gattgttaaa cgaagctatt 540
gtcaaagcag gtggtccaga taatttgtta acagccgttg ctgaacctag tatcgaagcc 600
gctaacgcaa tgatgagaca tccaggtatt caaatgttgg ttgctactgg tggtcctgcc 660
gttgtcaagg ctgtcttatc ttcaggtaaa aaggcaattg gtgcaggtgc cggtaatcca 720
cctgccgtag ttgatgaaac tgcagacata gccaaagcag ccaaggatat cgttgcaggt 780
tgttcattcg acaataactt gccatgcatt gctgaaaagg aaattatagc agttggtaga 840
atagccgatg aattgatctc ctatttgcaa aagtacggtg catacttaat aagtggtaga 900
gacatagaaa gattgatgga agtcgtatta acagaaagaa ccgaagaaat ggctccaggt 960
tgtgttggta aacctagaag agtctacggt gtaaataagg attacattgg taaagacgcc 1020
aagtttatat tgtctaaaat caacatacaa gctccagatc atataagagt tatcttatgc 1080
gaaacaccag ctgaccaccc tttcgtcttg gaagaattaa tgatgccagt attgccttta 1140
gtttcagtca gagatattga cgctgcaata gatttggcag taaaagttga acatggtaat 1200
agacacaccg ctgtcatgca ttctaagaac gtagactata tgactagatt agctaaggca 1260
atcgaaacaa ccatattcgt aaagaatgca ccatcatacg ccggtatagg tgttggtggt 1320
gaaggtttta ctacttttac tattgctggt cctactggtg aaggtttgac atccgcaaga 1380
agttttacta gacaaagaag atgcgcttta gttgatgcat tctctattgt ctga 1434
<210> 5
<211> 1395
<212> DNA
<213> AADHbs
<400> 5
atggatgaaa taatgataaa gaacatagta agagaagtat tgatgaaaac taatttggat 60
atctcctgta agagtgacca aggtgtattt gaagatgtta atgacgcaat tgatgctgca 120
tatattgccc aaagagactg gtatatgaac tacaaggtcg aagatagaag aagaatcatc 180
gaagctatca gagttgtcgc tagagaaaat gcaccaatgt tcgccaagat gattcatgaa 240
gaaaccggta tgggtagata cgaagataag atcactaaga acttggcagt tatagataaa 300
actccaggtg ttgaatgttt ggtcacagac gccatctctg gtgactcagg tttaatgatt 360
gaagaacaag ctccttttgg tgttattggt gcaataactc catctacaaa ccctacagaa 420
accatcatta ataacacaat ctcaatgata ggtggtggta atgcagtagt tttcaacgtt 480
catccaggtg ctaaaaaggt ctgtgcagta tgcttgcaaa tcttgcacaa gactatcgtt 540
gaaaacggtg gtccagctaa cttaataaca atgcaaagaa agcctgatat ggaagccgtt 600
aataagttga cagcttcccc taagattaga ttaatggttg gtaccggtgg tatgggtatg 660
gttaacgcat tgttgaagtc tggtaaaaag acaattggtg ctggtgcagg taacccacct 720
gtcgtagttg atgacaccgc tgatttggac aaagccgctt ccgaaatata tagaggtgcc 780
agtttcgata acaacttgtt gtgtttggct gaaaaggaaa ctttcgtaat ggacaatgtt 840
gctgatgaat tgattagaaa gatgtgtgcc tgcggtgctc atttgataac cccacaagaa 900
actgaacaat tgttgaaagt tattttcttg gaaaaggacg gtaaatactc tgttaacaaa 960
aagtgggtcg gtaaagatgc ttctttgatc ttagaatcaa taggtatcaa ggatgcagac 1020
acaagattgg tcttatgcga agtaccacat gatcaccctt ttgttttggt cgaacaatta 1080
atgccaataa tgcctatcgt cagatgtaaa actttcgaag attgcgtaaa gtatgcagtc 1140
gtagccgaaa atggtaacag acataccgcc tccatgttca gtaaaaatgt tgatcacatg 1200
actagattcg ctaaggaaat cgaaactaca atattcacaa agaacgcagc caccttggta 1260
ggcgttggta taggtggtga aggtcacacc actatgacta tcgcaggtcc tacaggtgaa 1320
ggtttaacct gtgccagatc ttttactaga agaagaagat gtatgttggc tgatggtggt 1380
ttaagagtta tttga 1395
<210> 6
<211> 1455
<212> DNA
<213> AADHbw
<400> 6
atggacgtca gacaacaaga tgtagaaaga atcgtagtag aagtattgaa aaagatgatg 60
agtgatcaac caaccgctgc tgccacaaca gttgtcgctg catccggttg tgattgcggt 120
gactttggtt tgttcgatag attggaagat gctgtccaag ccgctgaagc agcccaaaag 180
aaaattagta cagtagcaat gagagataag attatagctg caataagaaa ggctggtttg 240
gaaaatgcca aagcttttgc agaaattgca cataacgaaa ccggtatggg tagagtctct 300
gataagatcg ctaagaatat cttggtatgc gaaagaactc ctggtacaga atgcttatcc 360
ccaatggcaa ttagtggtga catgggtttg actttaatag aaaatgcacc ttggggtgta 420
atcgcctctg ttaccccttc aactaaccca accgctactg ttataaataa cgccatctcc 480
atgattgctg gtggtaatag tgttatcttt gctccacatc ctaacgctaa gagagcatct 540
caaactgcaa tccaagtatt gaataaggct atcatagaag caacaggtgt tgccaacttg 600
ttagtcgctg taaaagaacc taccattgaa gttgcacaag aattattctc acacccaaga 660
attaaattgt tagtagttac tggtggtgaa gccgtcgtag cccaagctag aaaagttgct 720
acaatgagat tgattgccgc tggtgcaggt aatccacctg ttgtcgtaga tgaaacagcc 780
aacattgcta gagcagccag atctatatat gatggtgcct cattcgacaa taacatcatc 840
tgtgctgacg aaaaggaaat tatagcagtt gattctattg ccgaccaatt aaaagctgaa 900
atgaaggcaa ttggtgccgt tgaaatatca ttggaacaag cagatgccgt cgctagagtt 960
gtcttaagaa attaccctca agttgaaggt ggtaaagctc caaatcctaa cccaaaatgg 1020
gtcggtagag atgctgcatt gatagcaaag gccgctggta ttgatgttcc agactcctgc 1080
agattgttaa tcgttgatgt caagagagac ataaaccatg tctttgctag agtagaacaa 1140
ttgatgcctg taattccatt gttgagagca gccaacgttg atgaagctat cgaatgggca 1200
ttgattttag aaagaggttt gtctcatacc gctggtatgc actcaagaaa tattgataac 1260
atggacaaga tggcaagagc catgaacact tcattattcg ttaaaaacgg tcctcacttg 1320
gctgcattag gtgctggtgg tgaaggttgg actacaatga caatttccac accaaccggt 1380
gaaggtgtta cctgtgctag aagttttgtc agattaagaa gatgttgcgt agttgataac 1440
ttcagaatag tttga 1455
<210> 7
<211> 1314
<212> DNA
<213> AADHcs
<400> 7
atgaagtcct ccatatactc cagtgtttcc gaagctgtaa tggctgctag aggtgctttt 60
aatagatacg ccgatttgac cttgaatgaa agaaaagaaa ttatagaagc tgtcaaggct 120
gcagtaagac caagaattac acaattggca gaaatgaccg ccgctgaaac tggtatgggt 180
aacgttgcag ataagatcga aaagatcaga ttggccgtcg aaaagactcc aggtgtagaa 240
gatttgatta cagaagtaaa caccggagat catggtatga ctttgtatga attatcagca 300
tttggtgttg tctgtgccgt tcacccatgt actaatcctt gcgctacatt gatttccaac 360
accatcggta ttttagcagc cggtaatgct gtagttcatt gtccacaccc tagagcagtt 420
agagtctctc aatacttaac tggtatgata tctgatgctg ttagagatgt ttgcggtatt 480
gataacttgg tcgtaactgt tggtgaatcc ttaatgggta gtacaagaga aatcatgtca 540
catccagatg ttgacttgat tgtctgcact ggtggttccg gtcctttaag acaagccatg 600
agtgctggta aaaaggtcat tggtgcaggt ccagccaatc ctgtagctat agttgatgaa 660
acagcagact tggaaagagc tgcaagagat attgttaaag gtgcttcttt cgacaataac 720
ttaatgtgta cctccgaaaa atctattgta gccgttgaaa agatagctga tgaattgatc 780
tctcatttgg aaagaaacgg tgtttactac actagatcag cagaagaaat agatagattg 840
tattgcaccg ccgtaactga agatttcgtt atcaacaaaa agatggaagg tcaatctgct 900
gatgttattt tggacgccgc taagatccca catagaaagg gtattaagtt gatcgtcgcc 960
gaaacagata gaacccaccc tttcgctaca ttggaattgt tgatgccatt gttgccttta 1020
gttagagcca gagattttga cgaagctttg gaagcagcct tagatatcga acaaggtttc 1080
agacataccg ctactattca ctctgaatca gttgaaagaa tgaatagagc tgctaagaaa 1140
ttgcaaactg cagtctttgt taaaaatggt ccatccttgt taggtattgg ttttgataag 1200
gaaggtcata cttcttttac tatcggtaca attaccggtg aaggtactac atctgctaga 1260
cactttgcta gaagaagaag atgtacttta acatctggtt tctcaattag atga 1314
<210> 8
<211> 1458
<212> DNA
<213> AADHho
<400> 8
atggcattga gagaagatag aatagcagaa atagtagaaa gagttttagc aagattggat 60
ggtaacagtg gttcctccgc cgcacctcat tctggttcag gtgctccagc tgcaaccgca 120
ggtggtgcct ctttggatat tcctagaggt actttaggtg tttatgctga tgcagacgcc 180
gctgtcaatg cagccagaag aggttttgct gcaaacgaag ctttgccatt aagaactaga 240
caagcaatga tagatgccat gagaaaagtt gctagagcac atatccctga attggctaga 300
tatgcagttg ccgaaacagg tttaggtaga tacgaagata aattggcaaa gaatgaatta 360
gttattgcca agacaccagg tcctgaaata ttggctccag ttgcatatac cggtgacgat 420
ggtttgacct taactgaaag agcaccatac ggtgttatag gtgcaatcac tccatgtaca 480
aatcctacag aaaccgttat ttgcaacgca ataggcatgt tgtccggtgg taatgccgtt 540
gtttttaatg ttcatccaag tgccgctaga gtctgtaatt ggttggtaca cttgttaaac 600
gaagcaatta tgtccgtcgg tggtccaaga gatgctatca cctccgtaga aagtcctact 660
attgacagtg cccaaacctt gatgactcat gctggtgtta gattagtagt tgtcactggt 720
ggtccaggtg tagttagagc agccatgaaa tctggtaaaa aggttatcgc tgcaggtcct 780
ggtaatccac ctgctgtcgt agacgaaact gccaacttgg ctaaagccgc tgcagccatt 840
ataaagggtg catcaataga taacaacatc atttgcacag ctgaaaaaga aatagtagct 900
gttgcttcta ttgctgacga attaagtaga ttgttaggtc aaagaggtgc tttggtatta 960
ggtgacgctc aagttagagc attggaaaga gttgtcttag acggtgaaca tgttaataag 1020
gaatgggtcg gtaaagatgc ctctagaatc gctgaacaaa ttggtttgag aggtcacggt 1080
tcagatttga gattgttagt atgtccagtt gacgaaggtc atccttttgt acaacacgaa 1140
ttgttaatgc cagttattgg tttagtcaga gtatccgatg ctactgaagc tatggctaca 1200
gcagttagag tcgaacatgg tttttgccac acagctgtta tgcattctac ccacattgac 1260
agattgtcag ctatggctag agtttgtaac gcatcaatat tcgttaaaaa tgattgcaac 1320
ttagctggtt tgggtttagg tggtgaaggt tttacatctt tcaccattgc atcaccaaca 1380
ggtgaaggtt tgactacagc tagagatttc actagagtta gaagatgtac attgaaggaa 1440
tcttttagat tcgtctga 1458
<210> 9
<211> 1404
<212> DNA
<213> AADHhs
<400> 9
atgaaaatca aagaaaacga attggataaa atagtcaacc aagtaatcag ttccttaaac 60
aacaagcaaa acagtaacga cttcaacact aagatcaatt acggtatttt ctctacaatg 120
gatgaagcaa ttgccgaagc tgttaaagca caagcctgtt tgcaattaaa ttactccact 180
gaagctagag aaaagattat taaatctatt agaaagaatg tttctaagca tgttgaaaag 240
atttctgaaa tggcagttga agaaacagat atgggtagaa tcgaagataa gatcattaag 300
aataacttgg ctattaataa gactccaggt acagaagatt tgagaaccga agcctttagt 360
ggtaaaaagg gtttgactat tgtcgaagaa gctcctttcg gtgtaatctg ttctattgca 420
ccagttacta atcctaccga aactataatc tctaacgcca tatcaatgat cgcttcctgc 480
aatggtgttg tctttaacag tcacccaggt gctaaaaagg tttctaagta cataatcgaa 540
gtcttgaata aggttattat ggaagccggt ggtccagaaa atttgttaac cgctgtcaac 600
gaacctacct tgcaaactgt agaatcttgt atgagagatg acagaattgc aatgatagtt 660
gccacaggtg gtccaggtgt agttaatgct gcattatctt caggtaaaaa ggctattggt 720
gctggtgcag gtaacccacc tgtcttggta gatgacacag ttgatttgaa gagagttgcc 780
aaagacatca ttaatggtgc ttcattcgat aacaacttac cttgcacttc cgaaaaggcc 840
attgttgctt tggaaagtat agctgattct ttgttaaacg aaatgacaaa ccaaaacgca 900
caattagttc atgacatcaa agccttggaa agagtcattt tgaacgatga cggtagtata 960
aacaaggcat tggttggtaa agatgccgct ttcattttga ataaggctgg tttaaaggca 1020
aagtctgaag atttgagatt agttattgtc gatgtagact taagacaccc atttgtccaa 1080
aaggaacaat tgatgccagt tattccttta gtcagagcta aaaatttcaa cgaagcaatg 1140
gaaatgggtg ttgatattga agaaggtaat agacatacag ctatcatcca ctcaaagaat 1200
gtagataact tgaccaagtt cgctaagaaa attgaaacta caatctatgt taaaaacgcc 1260
ccatcctacg ctggtattgg tgcaggtggt gaaggttacg caacattcac catagccggt 1320
cctacaggtg aaggtttaac ctccgctaga agttttacta gaaaaagaag atgcgttttg 1380
gtcgatggtt tctcaattat atga 1404
<210> 10
<211> 1398
<212> DNA
<213> AADHma1
<400> 10
atgcaaaccg atgctcaaca aatagaaagt atagttagaa gagtcataga acaattacat 60
tccccacaaa gagatggtga aagttacggt gtctttagaa ccttggatga cgcagtagcc 120
ggtgctcaag gtgcttacaa aaagataaga actatggctc aaagagaagc aattatagct 180
gcaatcagaa gaactggtag tgaaaatgtt caagcattgt ctgaattagc cgtccaagaa 240
acaggtttcg gtagagtaga agataagatc agaaagcata gattggtttt agacaaaact 300
cctggtatcg aagctattgt tccaatggca gtcacaggag atcatggttt gtctttaatt 360
gaaaatgctc cttggggtgt aatagcatcc gttaccccta gtactaaccc atctgctact 420
atcttgaaca acgcaatatc aatgatcgcc gctggtaatt cagttgtctt ttccccacat 480
cctgcagcca gagctgtctc ccaaagaaca atccaattga taaatagagc ctctgtttca 540
gctggtggtc ctgcaaactt agtcacctgt gtagaagaac caacaattga agctgcaacc 600
agattgtttt cattccctgg tatacaattg ttaaccatca ctggtggtga aggtgtagtt 660
aatgccgcta gaaaagttac tgataagaga ttaatcgcag ccggtccagg taacccacct 720
gtcgtagttg atgaaacagc tgacattgaa agagctgcaa tttcaatagt tcaaggtgca 780
tccttcgata acaacatcat atgtgttgac gaaaaggaaa taatcgccgt cgaatccatt 840
gctactgaat tgaaaactgc tatgtgcaga catggtgctg ctgaaattaa tgcagatcaa 900
gcagacgccg tcgctagatt ggtattagct ggttacccag gtcctaaccc acaccctaaa 960
ccagaatggg ttggtagaga tgctgaaaag attgcagccg ctgcaggttt tagtgtacct 1020
gcaggtacta gattgttagt tacagaaacc gaaagagatc atgcattcgc cactacagaa 1080
atgatgttgc cagttatctc tttgataaga gctagagatg cagaccaagc cattgattgg 1140
gcagttgaat tggaagccgg taatagacat acagccgcta tgcactcaag aaatatcgac 1200
aacttgtcca gaatgggttt agaaattaat tgttctttgt tcgttaaaaa cggtccttgc 1260
ttggccggtt taggtgctgg tggtgaaggt tggacaagta tgaccatatc tactccaaca 1320
ggtgaaggtg taaccaacgc tagtactttc gttagaaaga gaagatgcac aatggttgat 1380
tctttcagaa ttgtctga 1398
<210> 11
<211> 1422
<212> DNA
<213> AADHma2
<400> 11
atggccttta atgaagattt gattgcctcc atagtagaac aagttttgaa ggaaatacaa 60
gatgatgacc acgttatcgc ctccgctaga caatccgttg tcaaagcaaa tgataactgg 120
ggtgtctata ctgatgtaga cgaagctatg gaagtagtta ataaggcaca aaaggaattt 180
gctgcaatgc caatatccaa gagagatgaa attataagtg ctatcagaac agcaggtgtt 240
gaaaacgctg aacatttggc aagaattgcc cacgaagtta ccggttatgg tagaatcgaa 300
gataagactg ttaagaatag aaacgccgct atgttaactc caggtataga agatttgcaa 360
aaggaatttt tgtctggtga cggtggttca gtcattgtag aatacggtcc tatgggtgtt 420
atagcttcct tgatgccaag tacccatcct actgcatttg ttataaatca cgctatcgca 480
atgttagcag ccggtaacgc aatattcatc tgtccacatc ctaaagccca agctgcaaca 540
ttggaagcta tgaaggcatt gaataaggcc attgttgaag ctggtggtcc aaaatacttg 600
atggttgcag tcgataaggc cgacatggaa acctccaaca aagtttatgc acatccttac 660
gttaagatgg tcgccgctgc aggtggtgaa ggtgttatca gagccgcttt aaaaagtggt 720
aaaaaggcta ttaccggtgg tccaggtaat ccacctgtcg tagttgatga aactgctgat 780
attgctaaag cagccaagga tatcattgac ggtgaatctt acgataacaa cgttttgtgc 840
atagcagaaa aggaagtctt tgtcgtagaa tcagttgttg atagattgat tgctgaaatg 900
caaaagaata acgcttactt gatcaagggt caagatgtag ctaatttgac aggtgttacc 960
attaaagacg gtaaaccaaa tggtcaattg gtcggtaaaa acccttctgt aattttgaag 1020
gaaataggta tcgaagttgg tgacgatgtt agaactgtta tatttgaagc tccacctgaa 1080
catccattgg taatgatcga acaattgatg ccagttttgc ctatagtaag agttaaggat 1140
ttcgacgaag ccgttaaatg ggcagtcaag gccgaacatg gtgacggtca cacagctata 1200
ttgcattcta acaacatcaa cagaattaca gaatatgcca gagctatgca agtcaccgta 1260
ttagtagtta acgcaccatc ttattcatgt atggacccac aatcaaaaac tttggtttac 1320
gctcacactt taacaggtcc aacaggtgaa ggtttttgca ctcctagaca tttcacaaga 1380
caagaaagaa ttgtcttagg tggtgctttt cacttcgttt ga 1422
<210> 12
<211> 1437
<212> DNA
<213> AADHmm
<400> 12
atggaccaaa aggaaattga aaacgtagta aaagccgtct tagcctctat gagtgcaggt 60
acacaaccag cagccgcaag tgccgcacca caacaagctg cagcctccca aaataacggt 120
tttggtgtat tcgaaagttt ggatgacgct gttttagctg caaaagaagc acaaaaatcc 180
ttgaaaactg ttgaaatgag aaatttgtgt atcggtgcta tcagaagagc cgctaccgaa 240
catgcaagag aattggctgt tttagcagtc gaagaaacag gcatgggtag agttgaagat 300
aaattggcta aaaatttggc ccaagctaac ggtactccag gtgtagaatg tttaagacca 360
gaagttttaa caggagatca tggtttgacc ttaatagaaa atgcagcctg gggtgttatt 420
gcttctgtaa ctccatcaac aaaccctgct gcaacagcca tcaataacgc tatctctatg 480
attgctggtg gtaattcagt catttttgca ccacaccctg ccgctaaaaa ggtttctcaa 540
agaacaatca ccatcttgaa tgaagctatt gttgcagccg gtggtccaaa taacttgtta 600
gtcactgtag ccaaacctga tatcgaaaca gctcaaagat tattcaagta tccaggtata 660
ggtttgttag ttgtcactgg tggtgacgct gtagttgaat ccgcaagaaa gcatacaaat 720
aagagattga ttgctgcagg tgctggtaac ccacctgtcg tagttgatga aacagcagac 780
atcgaaagag ccgctaaagc cattgttcac ggtgctagtt tcgataacaa catcatctgt 840
gctgacgaaa aagttttgat cgcagtcgat tgcattgccg acaagttgat cacagaaatg 900
caaagaaacc atgcagtttt gttgaccaga gaacaatctg aaaagttgat ccctgtattg 960
ttaaagaacg ttgatgaaac cggtcacggt actgtctcaa gagattgggt tggtagagat 1020
gctgccaaaa tagctgcagc catcggtatg actgttccag cagatacaag attgttaatt 1080
gccgaaaccg actgtaagca tccttttgct gtcactgaat tgatgatgcc agtattgcct 1140
atcataagag taaaggatgt tgaccaagca atagatttgg ccgttaagtt agaaggtggt 1200
tgtcatcaca ctgctgcaat gcactccaac aacatcagta atttgaacag aatggcaaac 1260
gccattgata catctatatt cgttaaaaac ggtccatgca tagctggttt gggtttaggt 1320
ggtgaaggtt ggactacaat gaccatcacc actcctactg gtgaaggtgt tacatgtgca 1380
agaacctttg tcagattaag aagatgcact atggttgatt cattcagaat tgtctga 1437
<210> 13
<211> 1434
<212> DNA
<213> AADHpa
<400> 13
atggaaatac aaaacttaga agaagatatc agaagaattt tatcagaaga attgaagaag 60
agtggtacct cacaaaccgc ctcaacctct gatgccggtc aaaatggtat attcaagact 120
gtagatgaag ctatcgcagc agctaaagct gcagaagatg tttacattga taaaacttta 180
gcctttagag aaaaggtctt gactgcaatt agagaaggtt tcagacctta tatagaaaag 240
atggcaaaag atatcaaaga tgaaactggt atgggtacag ttgaagcaaa aatcgctaag 300
ttgaataacg ccttgtacaa tacccctggt actgaaattt tgcaacctga agctgaaaca 360
ggtgacggtg gtttggttat gtatgaatat gctccttttg gtgttatcgg tgcagttggt 420
cctagtacaa atcctagtga aacagttatc gccaacgcta ttatgatgtt ggccggtggt 480
aatacattat attttggtgc acacccaggt gccaagaaaa tcactagatg gaccatagaa 540
aaattgaacg aattggttta tgaagctacc ggtatgaaaa acttagttgt ctccatcgaa 600
gaaccatcca tcgaatccgt tcaagaaatg atgcaacatc cagatatcgc tatgttgtca 660
atcacaggtg gtccagcagt cgttcatcaa gccttggttt caggtaaaaa ggccgttggt 720
gcaggtgccg gtaatccacc tgctattgtt gatgctacag caaatgttgc tttggcagcc 780
cataacattg ttgattcagc ctcctttgac aataacatat tgtgcacagc tgaaaaggaa 840
gttgtcgttg aaagttccgt taaagacgaa ttgattaaga aaatgcaaga agaaggtgct 900
ttcttggtta ctaatgcatc cgacattgat aagttggccg aaatgacaat cggtaaaaat 960
ggtgcacctg acagacaatt tgtcggtaaa gatgcaacct atatattgga caaggcaggt 1020
attgcttaca ctggtacacc aaagttaatc attttggaag cccaaaaaga ccatcctttg 1080
gttactacag aaatgttgat gcctattgtt ccagtcgtat cctgtcctac atttgaccaa 1140
gtcttagcaa cagccgtaga agttgaacaa ggtttgcatc acacagcatc aatccactcc 1200
gaaaacttgc ctaatatcaa tagagcagcc cacagaatga atacatctat cttcgtcgtt 1260
aacggtgcca catacgttgg tacaggtgtt ggtgctaacg gtgcacatgc tggtgcatct 1320
gcattgacta tcgccacacc aacaggtgaa ggtacagcaa ccgctaaaac ctttacaaga 1380
agaagaagat tgaacagtcc agaagccttt agtttgagaa gttgggaagc ctga 1434
<210> 14
<211> 1434
<212> DNA
<213> AADHpb
<400> 14
atgcaaacta cagaagccac tatcagaaac gtcgtccaag aagtattagc acaattagcc 60
aagcaatcaa atggttcaaa cggtgttcca agagcagaaa gaaaagccgg tgactggggt 120
gttttcgatg acactgacca agccattgct gcagccaatg aaggttttag agctttatct 180
tctaagggta tggaagaaag agcaaagatc gtagaaatcg ttaagagaat ctgtgctgat 240
caagcagaag aattgggtag attggaattt caagaaacca aagtcggtag attggaccat 300
aagatcgaaa agttgcaaat tattaagaaa gtaccaggta cagaatggtt gagaaccgaa 360
gctatgtccg gagatcatgg tttgacagtt attgaatatg caccttttgg tgtaatcggt 420
acaattaccc cagttactca cagtttgcct acattagctg gtaatgttat aaacatggtc 480
gctgcaggta atactttggt tgtcaaccca catccttctg gtaaaaagat tgcctgtgaa 540
ggtgttagaa gattcaatag agctatatac gccgctacag gtttggaaaa tgtaataacc 600
atcgtttcca acccaacttt agaaagtgct gatagaattt ttaaccatag agatgttaag 660
atgttatgcg taactggtgg tccaggtgtt gctagagcag ctatggcttc tagaaagaaa 720
gccgtagttg ctggtcctgg taatccacct gttttggtcg atgaatccgc ttgtttacaa 780
aacgctgcag aaagtatcat tgccggtgct ggttatgaca acaacttgtt gtgcataggt 840
gaaaaggaag tattcgtcgt agaatccgtt tttgatagat tcatgcaaga aatggacaag 900
gcaggtgccg ttagattgaa tgcatctcaa gtcgatcaat tatcaaaagt agcctttaca 960
ccacctgaaa acgaaaagga tcattaccac gttaacagag acttggtcgg tcaacatcca 1020
tacgttttag ctaaacaaat cggtttgcca ttacctgaat caacaccatt gttatttggt 1080
gaaaccgatg aacacaatcc tttcgtcgct gaagaacaaa tgatgccatt tgtccctata 1140
gtaagatgta gaaacgcaga acatggtatg gatttgtgcg aaaagtatga acatggtttc 1200
agacacactt ctttaatcca ctcaagaaac gttagaaaca tgtctatcat gggtaaaaga 1260
atggatacta cattgtttat caagaatggt ccatcacctg ccggtttggg tttaggtggt 1320
gaaggttact tatccttcag tattgcaact ccaacaggtg aaggtgttac cactcctttg 1380
acctttacta gacaaagaag atgtacaatg gttgatgact taagaattgt ctga 1434
<210> 15
<211> 1359
<212> DNA
<213> AADHpe
<400> 15
atggaaatca aagaaatggt cgccttagcc agaaaagccc aaaaagaata tcaagcaaca 60
cataatcaac aatcagtaga caacatatgt agagctgcag ccaaagttat atatgaaaac 120
gctgaaatgt tggcaagaga agccgttgac gaaactggta tgggtgttta tgaagataaa 180
gtctccaaga atagaggtaa atctaagggt gtttggtaca acttgcatga caaaaagtct 240
gtcggtatat taaacattga tgaaagaacc ggtatgatcg aaattgccaa acctatgggt 300
gttgtcggtg ctgtcacccc aactacaaat cctatagtaa ctccaatgtc taacatcatc 360
ttcgccttga aaacttgtaa cgctatcatc atctcaccac atcctagatc taaaaagtgc 420
tcaaaacacg cagttgattt gatcttagaa gccattaagc ctttcaacgt cccagagggt 480
atggttcaag tcatagaaga accttccatc gaaatgacac aacaattaat gggtgcagta 540
gatgtagttg tcgcaaccgg tggtatggct atggttaaat ctgcttattc ttcaggtaaa 600
ccatcatacg gtgttggtgc tggtaacgta caagttattt tcgattctga catagatttc 660
gaagatgctg cagaaaaagt tatatatggt agatcctacg ataatggtat catatgtact 720
ggtgaacaat ctgttatata taacgaagcc gacaaagatg ctgtttttgc cgctttaaga 780
aagagaggtg catacatttg cacagaagaa gaaggtaaaa agatgagaga cttgttgttc 840
caaaatggtg gtcctattaa caaagatgta gttggtcaag gtgtccaaaa gattgctaag 900
atggcaggta ttaatgtacc agaaggttct agagtcataa tcgtagaagc aaacggtgta 960
ggtgaagaag atgttatctg tagagaaaag atgtgtccag ttatgtgcgc tttgtcatac 1020
aagcatttcg aagaaggtgt tgatatcgca tgtagaaatt tggccaacga gggtaatggt 1080
cattcctgcg ctatacacag taacaaccaa gcacatatca tcttgactgg ttctaaagct 1140
acagtttcaa gaattgtcgt aaatgcccct tcagctacca ctgcaggtgg tcacatacaa 1200
aacggtttga atgttacaaa caccttaggt tgcggttcct ggggtaacaa ctccatcagt 1260
gaaaacttca catacaagca cttgatgaac attagtagaa tagctccatt gaaccctgct 1320
attaaagttc caaacgatca agaaatctgg gaattatga 1359
<210> 16
<211> 1383
<212> DNA
<213> AADHrw
<400> 16
atgaccgtta ccgctacatt ggacgcagat ttggcagcat tggcagacgt tagagcacaa 60
gtatccgcaa ctaagaaggc atttgacgcc atcgaaggtg ctgatcaaca atggttggac 120
gaaattgtta gagctatggc taaagcaggt tatgatgctt ctgaagaatt agccagaatg 180
gctgtagacg aaacaggtta tggtgtttac gaaaataaga ttattaaaaa tagattcaat 240
accggtttcg ttgctgattg gatgttggaa agaagagccg taggtgtttt atgggctgac 300
gaagaaaaca aggttactgc agtcggttct ccaatgggtg tcatagctgc aatcattcca 360
gtaacaaatc ctactgccac agttttgttt aaatccttgg caagtgttaa ggctggtaac 420
gccgttgtcc atgctccaca ccctagagcc gctagatgtt ctgcaagagc agccgaaatt 480
atggccgaag ctgcagtcca tgccggtgct ccagaaaatt tggtttcatg cttaagacaa 540
cctacattgg atgctaccag agaattaatg agacacggtg acattagatt ggttttagct 600
actggtggtc cagcaatggt tgccgctgca tattcttcag gtaaaccaac catagctgtt 660
ggtgctggta atgtccctgc ttacgtagat tccactgtta aagacttggc cgaaaccgct 720
actatgatag ttacatccaa gagtttcgat aacggtaccg catgtgttgc cgaacaaagt 780
gtagttatcg ccgctccagt tgctgatcaa ttggtccaag aattaggtag agcaggtgcc 840
acctggatga ctaaagatca acaagcagcc ttgacaagaa ccttatttga cgaaagaggt 900
ggtttgagag ctaatgcagt aggtcaatct gcagttagat tagccgaaat gtccggtttc 960
actgtccctg ctagtacaaa ggtattggct gcagaattag atgaagtcgg tgaccatgta 1020
ttgttatcaa gagaaatctt gggtccagta ttgactgttt acagagaacc tgattaccaa 1080
gcaggttgga gaagatgtag agaaattttg gctttaggtg gtgaaggtca taccgtcgca 1140
gtacacactt ccgatccaga agtcgtagct aaatttggtg aattgccagc aggtagaatt 1200
gttgtcaata ctcctgcttt atttggtggt atgggttact ctacaaacgt tgatccttca 1260
ttccaaattg gtactggtac atggtctggt tcaatatgct cagacaatgt tacaccattg 1320
catttggtca acataaagag aatcgctcac gaagcaagac cttggagagg tgttttcgat 1380
tga 1383
<210> 17
<211> 2616
<212> DNA
<213> AADHsl
<400> 17
atggctattg aagaacactc attgaacgac aaggaatcca agaaagaaac cttagaagaa 60
accattgact tattagtaac aaacgctaaa acagcttctg aagagtttac tccattggat 120
caacaacaaa tagacgaaat cgttagacaa atggcattgg ccggtttaga tcaacatatg 180
caattggcaa agatggccgt cgaagaaaca aagagaggtg tatacgaaga taagatgatt 240
aagaatatat ttgctacaga atacatctat aactctatta agaaaaataa gaccgtaggt 300
gttatttcag aagatccata cgaagaaatc gttcaaattg cagaacctat aggtgttatt 360
gctggtgtca cccctgtaac taacccaaca tctactacaa tgttcaagtc cttgattagt 420
ataaagacta aaaaccctat aatctttgcc ttccatccat ccgctcaaaa gtgtagtgct 480
gcagccgcta aagttgtcag agatgcagcc gttaaggctg gtgctcctaa aaattgcatt 540
caatggatag aaactccttc attggaagca acacaaagat taatgcatca cccaggtgta 600
tctttgattt tagctacagg tggttcaggc atggttaagg ctgcatattc ttcaggtaaa 660
cctgcaattg gtgtaggtcc tggtaatgtt ccagcctaca tagaaaagtc tgcagatgtt 720
aaacaagccg tcaacgactt gatcttgtcc aaaactttcg ataacggtat gatctgtgct 780
agtgaacaag cagtcatcgt agaccaacaa atctatgaag ctgttaaaaa ggaaatgatt 840
gataataagt gttacttctt gaacaacgaa gaaaagaaga aagttgaaaa gttagttatt 900
gatgaaagaa gatgcgcagt caacccacaa attgtaggta aaactgctgc ttttattgct 960
ggtttggcag gtgttacagt ccctgaagat accaaagtaa ttgttgctga attgtccagt 1020
gttggtccag aatctccttt atcaagagaa aaattgtcac cagtcttagc ctgttttaga 1080
gctaagacca ctgaagaagg tttcaaaaga tgcgaagaaa tgttagcatt tggtggtatt 1140
ggtcattctg ccgtaataca ctcaaacgat caaaaagtta tagacgcctt cgctatgaga 1200
atgaaggctg gtagattgat cgttaatgca ccatcttcat taggtggtat cggtgacatc 1260
tataaccatt ttattccttc cttgacctta ggttgtggta gttacggtgg taattccgtc 1320
agtaccaacg taggtactgc taatttgatc aacatcaaga ctttggcaaa cagaagaaac 1380
aacatgcaat ggttcaaggt tccacctaaa atctatttcg aaaagaacgc taccaaatac 1440
ttgtctgtta tgccagatat aactagagct tttattgtag ccgaccctgg tatggttaaa 1500
ttcggttatg ttgacagagt cattcatttc ttgaagcaaa gaagagatcc agttcactac 1560
gaagttttct ctgatgttga accagaccct tcagtagcta ctgttatgaa gggtacagaa 1620
ttgatgagaa aatttgaacc agacgttatc attgctttag gtggtggttc tgcaatggat 1680
gcagccaagg gcatgtggtt gttctatgaa tcacctgaaa cagatttctt tggtttgaag 1740
caaaagttct tggacataag aaagagagtc tttaaattcc cagatttggg taaaaaggct 1800
aaattcgtag caatccctac aacctccggt accggtagtg aagttacttc tttttcagtc 1860
ataactgata aggaaaacga catgaagtac ccattggccg actacgcttt aactcctaac 1920
gttgcaataa ttgatccaga atttgtcttg acagtacctc aaagagtctt agctgattcc 1980
ggtatggacg tattgactca tgcaattgaa gcctacgttt ctaacatggc taacgatttc 2040
acagacggtt tatgtatcaa ggcaatccaa ttggttttta aatatttgcc aagatcttac 2100
aaggattcaa ccgacagaga agccaaggaa aagatgcaca atgcttccac tatcgctggt 2160
atggcttttt ctaatgcctt cttgggtatt aaccattctt tggctcacaa attgggtaat 2220
gaatttcatt tggcccacgg tagatgttgc gctatcttgt tgcctcatgt tataagattc 2280
aacgctcaaa agccaacaaa atttgctgca ttccctaagt acaagcactt tattgctgat 2340
gaaagatacg ccgaaatagc tcaagttttg ggtatcgcag gtaaaactac agaagaatct 2400
gttgaaaatt tggtccaagc tattaagaaa ttgactcaag aattaaacat gccaatgtcc 2460
atcgaaaaga acggtgttag taaagaagct ttcgaagccg ctgtcgataa attagcagac 2520
agagcctttg gtgaccaatg cacaccagca aaccctaaga tgccattggt ttcagaatta 2580
accgaaatct atagacaagc ttttaaaggt atttga 2616
<210> 18
<211> 1389
<212> DNA
<213> AADHss
<400> 18
atggcagatg aacaattagt ccaaaatgta gtaagagaag tagtagcaag aatgcaaata 60
tccgctccag caagaggtat gcacggtgtt ttctctgata tggaagaagc aattgaagct 120
gcaagaactg cccaacaaac agttagattg ttaccaatgg accaaagaga aaagattatc 180
ggtgccataa gaagaaaaac tagagaaaac gctgaaatct tggcaagaat ggccgtaaat 240
gaaacaggca tgggtaacgt tggtgacaaa attttgaagc atttgttagt cgccgataaa 300
gtaccaggta ccgaagatat ctctactaga gctttttcag gtgacagagg tttgacttta 360
attgaaatgg gtcctttcgg tgtaatcggt gctattaccc catgtactaa tccttccgaa 420
acagttttat gcaacaccat cggcatgttg gcaggtggta atacagttgt ctttaaccca 480
catcctcaag ccataaagac tacattattc accatccaaa tggttaatga agcttcattg 540
gaagcaggtg gtccagataa catagcttgt acagttgacg cccctacatt agctacctct 600
gaaatcatga tgaagtcacc acacatcaag ttgttagtcg caaccggtgg tcctggtgta 660
gttactgctg tattatcttc aggtaaaaga gcaattggtg ccggtgctgg taatccacct 720
gccttggtcg atgaaaccgc tgacatcaga aaggccgctg aagatatagt taacggttgt 780
actttcgaca acaacttacc atgtattgct gaaaaggaaa ttgtcgcagt agattctatt 840
gccgacgaat tgttgcatta catgttgact gaacaaggtt gttaccaagc ttcagaagaa 900
gaattggata gattaacaaa ggcagttatg gacgaaaagg gtagattgaa tagaaaagct 960
gtcggtagat ccgcaagaaa gttgttaagt atgatcggtg ttgaagtcga tgctaacata 1020
agatgcatca ctttctttgg tccaaaggaa caccctttga taaccactga attaatgatg 1080
ccaatcttgg gtatcgtaag agttaaggat tttgcagaag gtttagaaac agcagcctgg 1140
ttggaacatg gtaataagca ctccgctcat atccacagta aaaacgttga tagaatcaca 1200
gaatacgcaa gaagattaga cacaaccatt accgttaaga atggtccttc ttatgctgca 1260
ttgggttttg gtggtgaatc ttactgtact ttcacaatag catcaagaac tggtgaaggt 1320
ttaacatccg ccagaagttt catcaaatcc agacattgtg ttatgactga tagtttgtgc 1380
gtcagatga 1389
<210> 19
<211> 1404
<212> DNA
<213> AADHta
<400> 19
atgaataaca ctgaattaga atccttgata agaaccattt tgaccgaaca attaacacca 60
tccgctactg atacccctgc ctgcactgcc tcttcagtcg ctttatttga tgacgtagat 120
tctgctattt gtgctgcaca tgccgctttc ttaagatatc aagaagcacc attgaaaact 180
agatctgcca ttatagcagc cattagagca gaaatagccc cttgcttatc agaattggca 240
gaaagagctg cagccgaaac tggtatgggt aacacagctg ataagatctt gaaaaataag 300
gctgcattgg aaaacacacc aggtattgaa gatttgaaaa ctacagcttt gaccggtgac 360
gaaggcatgg ttttgttcga atactctcct ttcggtgttg tcggtgcagt cgccccatca 420
acaaacccta ctgaaacaat cattaataac tccatcagta tgttggccgc tggtaatgct 480
atctattttt ccccacatcc tggtgcaaag aacatcagtt tgtggttgat ccaaaagatg 540
gaagaaatcg cttttaaagt ttgtggtatc cataacttga ttgtcaccgt aaaggaacca 600
acattcgaag ctacccaaca aatgatggca cacgataaaa ttgctttgtt agcaataact 660
ggtggtcctg gtatagttaa catgggttta aagtctggta aaaaggtcat aggtgctggt 720
gcaggtaacc caccttgctt ggttgacgaa actgcagaaa ttgtcaaggc agcccaagat 780
atagtagccg gtgcttcctt tgactacaat ttgccatgta ttgctgaaaa atctgttatt 840
gctgttgatt gcattgccga ccaattgata caacaaatga gagaatttgg tgccatgcaa 900
atcacagatc cacaacaaat tgctcaatta agagaagtct gtattcaaaa aggtgctgca 960
aataagtcct tagtaggtaa aagtccagct accatattgg ccgctgcagg tatcccatgc 1020
cctgcaaagg aacctagatt aataatcttg gaagttccag ctaacgatcc tttcgtagtt 1080
actgaacaat taatgccagt tttgcctatt gttagagtcg ataacttcga acaaggtttg 1140
caattagctt tgaaagttga agatggtttg catcacactg caatgatgca ttctcaaaat 1200
gtttcaagat taaacaaggc cgctcacttg atgcaaacat ctatattcgt taaaaatggt 1260
ccatcatacg ccggtatcgg tgttggtgct gaaggtttta ccactttcac aatagcaacc 1320
cctactggtg aaggtacaac ctccgccaga acctttggta gattaagaag atgtgttttg 1380
actaacggtt tctcaattag atga 1404
<210> 20
<211> 1425
<212> DNA
<213> AADHtl
<400> 20
atggtagaca tagacttaaa gaacgtagaa caaatcgtca gaactgtatt agcctcaatg 60
caaactcaac ctcaagccgt ccaacctgct gcaggtagaa gagccgctcc aggtgttttt 120
actgaattgg atgacgccgt tgctgaagca tctacagcac aaagagccat tagaaatttg 180
gctaccagag ttaaagttgt cgaagcaata agaaaggcag ccgaagccca tgctagagaa 240
ttggctgaat tagcagtcgc cgaaacaggc atgggtagag tagaagataa attcgccaag 300
aacgtttcac aagctagaca cacaccaggt gttgaatgtt tggctcctac cgcattaact 360
ggtgacagag gtttgacctt aattgaaaat gctgcatggg gtgtcatagc ttccgtaaca 420
ccaagtacca accctgccgc taccattatt aacaacgcaa tttctatgat ctcagccggt 480
aatactgtag tttttgctcc acatcctgca gccaaaaagg tttctcaaag aactgtctca 540
ttgttaaacg aagcatccgt tgctgcaggt ggtccagata atgtcgtaac tacagttttg 600
aaccctgata ttgctacagc acaaagatta ttcagttatc caggtattca tttgttaaca 660
gttaccggtg gtgaagctgt tgtcgaagcc gctagaaaac acactgataa gagattgatc 720
gcagccggtg caggtaatcc acctgtagtt gtcgatgaaa cagccgactt agctagagct 780
gcaagagata tagtttgggg tgcttccttc gataacaaca tcatctgtgt tgacgaaaag 840
gaaatcatcg tagttgatag agtctgcgac gcattgaaaa ctgaaatggc aagacataag 900
gccgttgaat tgacaccaga tcaaggtaaa agattagttg acgtcttgtt gaactatgtt 960
ggtgctgatg gtagaggtac tgtatctaga gattgggttg gtagagatgc tgctaagatc 1020
gcagccgcta ttggtttgga agttccagct gataccagat tgttatacat tgaaactcca 1080
gctgaccacc ctttcgcagt cacagaatta atgatgccag taatacctgt tgttagagca 1140
ccagatgttg atgcagccat tgatttggca gtaaagttag aaggcaactg tagacatacc 1200
gctgcaatgc actctagaaa cattgatgct ttagacagaa tggccaacgc tataaacaca 1260
tcaatcttcg ttaagaatgg tccatgcttg gcaggtttag gtttcggtgg tgaaggttgg 1320
accactatga ctatttccac tcctacaggt gaaggtgtta cttctgctag aacatttgtc 1380
agattgagaa gatgcgtatt agttgatcat ttcagaattg tttga 1425
<210> 21
<211> 2739
<212> DNA
<213> AADHtm
<400> 21
atggatacag ccgtagccgt tcaaaaacaa gatattgcca tgtctgccgc cagtaagcct 60
aagtctgcct caggtcctcc tttcgatgtc caagcacatg tagatggttt agttgcaaga 120
gccattagag ctttagcaga atttcaagct ttggatcaag aacaagttga tagaatagta 180
gccaaggctt cagttgctgc attaaatcaa cacggtgttt tggcaaaatt agccgtcgaa 240
gaaacaggta gaggtttgtt tgaagataaa gcagtcaaga acatgttcgc ctgtgaaaac 300
gtaacatctg caatgaagag aatgaaaact gtaggtgtta ttcatagaga taacttgaac 360
ggtatagtag aaatagctga accagttggt gttgtcgccg ctgtaactcc tgttacaaac 420
ccaaccagta ctgcaatttt taaggccttg gtttgtttaa aaactagaaa ccctatcgtc 480
tttggtttcc atccacacgc tcaaaagtgc tctgcagaag ccgtcagaat tgtaagagat 540
gcagccgtcg ctgcaggtgc tccaaccaac tgtgcacaat ggatagaagt tccttcaatc 600
aaagctactg gtgcattaat gaatcaccca gatatctccg taattttggc caccggtggt 660
gacgctatgg ttaaggccgc ttattcatgc ggtaaacctg ctattggtgt tggtcctggt 720
aacgttccag catttgtcga aaagactgcc gatgttgcta gagcagccta tgacttggtc 780
ttatctaaat cattcgatta tggtatggtc tgtgcatcag aacaagctgc aattatagaa 840
gccccagtat acgaccaagc tatggcagaa atgcaaagat tacatgcaca cgttgccact 900
tctgatgaaa agagaaagtt ggaagaatta gcatttggtg tagttgccaa cagtgaaaat 960
tgcggttctg ctaagttgaa cccagcaatc gttggtaaat ccgctagttg gttggctaaa 1020
caagcaggtt tcagtgttcc tcaagataca tctatcttgt tagtagaagc agatcatgtt 1080
ggttatgacg aaccattgac cagagaaaag ttgtgtccta tcttggctgt tattagagcc 1140
agagatgctg atgacggtat cagattatct gaacaaatgg ttgaacaaga cggtttgggt 1200
cattcagcat gcattcactc cactgatgaa gaagtcatcg aaaagttcgg tagaagaatt 1260
aaagctgtaa gaatcataac taactctcca acatcattag gtggtatagg tgacatctat 1320
aatgctttcg ttccttcatt gacattaggt tgtggtacct acggtaaaaa ttctgtttct 1380
aacaacgttc aagctgtcaa cttgattaat gtcaagagaa tcggtcatag aaacaacaac 1440
atacaatggt ttaaagttcc tgctaagatc tatttcgaac caaacgccat cagatactta 1500
gctgacatga gaagagtaaa cagagttttt atcgtcaccg atgctactat gtcaaaattg 1560
ggtgtcgtag atagaattag agatgttttg aacagaagat taaacatggt taccatgcaa 1620
gttgtcgata ctatagactc tgaaccatcc atcagtatgg tcgaagtagg tgctgaacaa 1680
atgagaaatt tccaacctga cacaattgtt gctttaggtg gtggttctgc aatggatgcc 1740
gctaaggtca tgtggttgtt atatgaacac ccagaagtct ctttttcaga tgtacgtgaa 1800
aagtgtttcg acgttagaaa aagagctttt actttccctg atttgggtga aaaagcacaa 1860
ttggtatgca tatccactac aagtggtact ggttctgaag ttacaccttt tgcttgcatc 1920
accgatccaa aaactggtta taagtaccaa ttgacagact acgccttaat gcctaccgta 1980
gctattgttg atccaacttt agttaacgct atgccttcaa gattggcagc cgattccggt 2040
atggacgcct tagctagagc aactgaagca tatgtttcag tctacgccaa cgattttaca 2100
gacggtttgg caatgcaagc cattaaattg atcttcgaaa atttgccagc atccgttttg 2160
aaaggtgacg ctaaggctag agaaaagatg cataatgctg gtacaatagc tggtatggca 2220
tccggtagtg catttttggg tatagttcat gcaatcacac acaccattgg tgcctcctat 2280
agaattagtc aaggtagatc taacgctact ttgttgccat tgggtattag atacaacggt 2340
agaataccaa caaagttgac ctcttggcct aaatatgaaa agtacgttgc tccacaaaga 2400
taccaagata ttgcaaagat gttgggttta cctgctgcaa ctccagaaga aggtgtcgaa 2460
agttatgcaa gagccgtaga agatttgaga gctacattga acatggaata ctcttttaga 2520
gcacaaggta tagaagaaca tgatttcgtt gctaacttag acgaaatggc cttcagagct 2580
tcagatgacc aatccgctcc agcaaatcct agattaccaa tggttgaaga tttgaagatc 2640
atcatggaag ccgcttatta cggtatcagt ttcgatgaca ttgtagccag aagagcctct 2700
gctcaattgg ctaaatctga agtttctaag aaaggttga 2739
<210> 22
<211> 1599
<212> DNA
<213> AADHvs
<400> 22
atgaacgaac aagaaatcgc ccacgcagta gaaaatgtat tatcaaagta caccaacgta 60
accgcacaaa acgccgaacc agtaagttat tcttcaaacg ctagtttgga aaacattgta 120
tctcaagcct tagctggtaa catggttaaa caaccagaaa cacaaaccgc acctgatttg 180
aactctaaca tcgaaaacat agtttcccaa atcttggccg aaaaccaagc taagccacaa 240
tcagttcaat gtcaatccgc taatcatggt actacagaat atttgggttg ctttgcctct 300
atggaagaag ctatttctgc tgcatcacat gcacaagttc aatacagaca ctgtactatg 360
ggtgacagag cctcatttgt caaaggtata agagaagtat tcactcaaga tgacgttttg 420
gaaaagatct ccagaatggc agttgaagaa accggtatgg gtaattacgc cgataaatta 480
actaagaaca gaatagccgc tactaaaaca ccaggtatcg aagatttgac cacttccgct 540
ttaagtggtg actctggttt gaccttaact gaattttctg catatggtgt tatcggttct 600
attacaccaa caaccaaccc tacagaaacc attattaaca actcaatcgg catgttggca 660
gccggtaata ctgttgtcta ctccccacat cctagaagta gaaatgtttc tttggttgct 720
gttgatttga ttaatagaaa gttggcagaa ttaggtgcac cagccaattt ggtagttact 780
gttttagaac cttcaattga taacacaaac gctatgatga acgacccaag agtaaacatg 840
ttagttgcaa caggtggtcc ttcaatcgtc aagactgtaa tgtccacagg taaaaaggct 900
attggtgctg gtgcaggtaa tccacctgca gtcgtagatg aaactgccaa catagaaaag 960
gctgcaaagg atatcattaa tggttgtgcc tttgacaata acttgccatg tattgctgaa 1020
aaagaagtca ttgttgtcaa tgaagtagcc gattatttga tacactgtat gaaaaagtca 1080
ggtgcttact tgttatgcga caagcaaaag atacaacaat tgcaaagttt ggttttgaac 1140
gaaaaaggta ccggtccaaa cacttctttt gtcggtaaag gtgctagata catcttggat 1200
aagttgaaca tccaagtttc tgatgacatt aaggtcatat tgatcgaaac agaaagaaat 1260
catcctttcg tagttcacga attgatgatg ccaatattac ctgtcgtaag agttgaaaat 1320
gtcgatgaag caatagactt agccatcaaa gttgaacatg gtaacagaca cacagctata 1380
atgcatagta ccaacgttga aaagttgtct aagatggcaa gattaatcca aactacaatc 1440
ttcgtcaaga atggtccatc ctatagtggt attggtgtag gtggtgaagg tcacaccact 1500
tttacaattg ctggtcctac cggtgaaggt ataacttcag ctagatcctt cgcaagatac 1560
agaagatgtg taatggttga agcattaaat attagatga 1599
<210> 23
<211> 2676
<212> DNA
<213> ADHEec
<400> 23
atggcagtca caaatgtagc cgaattgaac gccttagtag aaagagtcaa gaaagcccaa 60
agagaatacg caagttttac acaagaacaa gttgataaaa tattcagagc cgctgcattg 120
gccgcagccg acgcaagaat ccctttggca aagatggctg tagcagaaag tggtatgggt 180
attgttgaag ataaagttat caaaaaccat tttgcttccg aatatatcta caatgcctac 240
aaagatgaaa agacctgcgg tgttttatca gaagatgaca catttggtac catcactatt 300
gcagaaccaa ttggtattat ctgcggtatt gttccaacca caaacccaac atcaactgcc 360
atcttcaaat cattaatatc cttaaaaact agaaatgcaa ttatcttttc acctcaccca 420
agagccaaag atgctaccaa taaggctgca gacatcgttt tgcaagcagc catagccgca 480
ggtgcaccta aggacttaat tggttggata gaccaacctt cagttgaatt atcaaacgca 540
ttgatgcatc acccagacat aaacttgatc ttagcaacag gtggtcctgg tatggttaag 600
gcagcctaca gttccggtaa accagcaatc ggtgttggtg ctggtaatac accagtagtc 660
atagacgaaa ctgctgatat caagagagct gtagcctcag ttttaatgtc caagacattt 720
gataatggtg ttatctgtgc atctgaacaa tccgttgtag ttgttgattc agtctatgat 780
gccgttagag aaagattcgc cactcatggt ggttacttat tgcaaggtaa agaattaaag 840
gctgttcaag atgttatctt gaagaacggt gctttgaatg ctgcaatagt aggtcaacct 900
gcttataaaa tagccgaatt ggcaggtttt tccgtacctg aaaacacaaa aatcttgatt 960
ggtgaagtaa cagtcgttga tgaaagtgaa ccttttgcac atgaaaaatt gagtcctact 1020
ttggcaatgt atagagccaa agatttcgaa gatgcagtcg aaaaggctga aaaattagtt 1080
gcaatgggtg gtatcggtca tacatcatgc ttgtacacag accaagataa tcaacctgct 1140
agagtaagtt actttggtca aaagatgaaa acagccagaa tattgattaa taccccagca 1200
tcacaaggtg gtattggtga cttatataac tttaaattgg caccatcatt gaccttaggt 1260
tgcggttcct ggggtggtaa ctcaatctcc gaaaacgtcg gtcctaaaca cttgataaac 1320
aaaaagactg ttgcaaaaag agcagaaaac atgttatggc ataaattgcc aaagagtatc 1380
tacttcagaa gaggttcctt accaatcgcc ttggatgaag ttattactga cggtcataag 1440
agagcattaa tcgttacaga cagatttttg ttcaacaacg gttatgctga ccaaatcaca 1500
tcagtattga aagccgctgg tgttgaaact gaagtctttt tcgaagttga agctgatcct 1560
acattgagta tcgttagaaa gggtgctgaa ttagctaatt cttttaaacc tgacgttatt 1620
atcgcattgg gtggtggtag tccaatggat gccgcaaaaa tcatgtgggt catgtatgaa 1680
cacccagaaa cacacttcga agaattagca ttgagattca tggacatcag aaaaagaatc 1740
tacaaattcc caaagatggg tgtaaaggcc aaaatgatcg cagttacaac aacatcaggt 1800
accggttccg aagtaacccc atttgcagtt gtcacagatg atgccactgg tcaaaaatac 1860
ccattagctg attatgcttt gacacctgat atggccatcg ttgacgctaa cttggttatg 1920
gacatgccta aatcattatg cgcttttggt ggtttggatg cagttaccca tgcaatggaa 1980
gcttatgttt cagttttagc ttccgaattt tctgatggtc aagctttaca agccttaaaa 2040
ttattgaaag aatacttgcc agcatcatat catgaaggtt ccaagaatcc tgttgccaga 2100
gaaagagtac attcagccgc aactatagcc ggtattgcat tcgctaatgc atttttaggt 2160
gtttgtcatt caatggccca caagttgggt tctcaatttc atataccaca tggtttggca 2220
aatgccttat taatttgtaa cgttatcaga tataacgcca atgacaatcc aactaaacaa 2280
acagcatttt cccaatacga cagaccacaa gcaagaagaa gatacgcaga aatcgcagat 2340
catttgggtt tgtctgcacc tggtgacaga accgcagcta agattgaaaa gttattagca 2400
tggttggaaa ccttaaaggc agaattgggt atcccaaagt ctattagaga agcaggtgtc 2460
caagaagcag atttcttagc caatgtagac aagttgtccg aagatgcatt cgatgaccaa 2520
tgcacaggtg caaatccaag atatccattg atctccgaat taaaacaaat attattggac 2580
acatactatg gtagagatta tgtagaaggt gaaaccgcag ccaagaaaga agcagctcca 2640
gctaaggcag aaaagaaggc taaaaagtcc gcttaa 2676
<210> 24
<211> 2658
<212> DNA
<213> AHEpm
<400> 24
atgtctggtt tacaaatgtt ccaaaacttg tccttatacg gttctttagc cgaaatagat 60
acctctgaaa aattgaatga agctatggac aaattgacag ccgcacaaga acaattcaga 120
gaatataacc aagaacaagt tgataagatc tttaaggccg tcgcattggc agcttcacaa 180
aacagagttg ctttcgcaaa atacgcacac gaagaaactc aaaagggtgt ttttgaagat 240
aaagttatca aaaacgaatt tgcagctgat tatatctacc ataaatattg taacgacaag 300
actgcaggta ttatcgaata tgatgaagca aatggtttaa tggaaatcgc agaaccagtt 360
ggtcctgttg ttggtatcgc acctgttaca aacccaacat ccactatcat atataagtca 420
ttaatcgcat taaagacaag aaattgcatt atattctccc cacatcctgg tgcccataaa 480
gcttcagttt ttgtagtcaa ggtcttgcac caagcagccg taaaagctgg tgcaccagaa 540
aactgcatcc aaataatctt tccaaagatg gacttgacta cagaattgtt acaccatcaa 600
aagaccagat tcatttgggc aaccggtggt ccaggtttgg ttcatgcctc ctatacttcc 660
ggtaaaccag ctttaggtgg tggtcctggt aatgctccag cattgattga tgaaacttgc 720
gatatgaatg aagccgtagg ttctatcgtc gtttctaaaa cattcgactg tggtatgatc 780
tgcgcaacag aaaacgcagt tgttgtagtt gaaagtgttt acgaaaactt tgttgcaact 840
atgaagaaaa gaggtgcata tttcatgact ccagaagaaa ccaagaaagc atcaaacttg 900
ttattcggtg aaggtatgag attgaacgct aaggcagttg gtcaaaccgc taaaactttg 960
gctgaaatgg ccggtttcga agttcctgaa aacacagttg tcttgtgtgg tgaagcttcc 1020
gaagttaaat ttgaagaacc tatggctcat gaaaaattaa ctacaatctt aggtatatat 1080
aaggcaaagg attttgatga cggtgttaga ttgtgcaagg aattggtcac atttggtggt 1140
aaaggtcata cagcagtttt atatacaaat caaaataata aggatagaat cgaaaagtac 1200
caaaatgaag ttcctgcatt tcatatctta gttgatatgc cttcttcctt gggttgcatt 1260
ggtgacatgt ataactttag attagcacca gccttgacaa tcacatgtgg tactatgggt 1320
ggtggttcca gttccgacaa catcggtcca aagcacttgt taaatattaa aagagttggt 1380
atgagaagag aaaacatgtt atggttcaaa attcctaaga gtgtttactt caaaagagcc 1440
attttatctg aagctttatc agacttgaga gacacccata agagagcaat aatcatcacc 1500
gatagaacta tgactatgtt gggtcaaaca gacaagatca ttaaagcatg cgaaggtcac 1560
ggtatggtat gcactgtcta cgataaggtt gtcccagatc ctacaatcaa gtgtatcatg 1620
gaaggtgtta acgaaatgaa cgtattcaag ccagatttgg ccattgcctt gggtggtggt 1680
tcagctatgg atgcagcaaa aatgatgaga ttattttacg aatatccaga tcaagacttg 1740
caagatattg ccacaagatt cgttgacatc agaaagagag tagttggttg tcctaaattg 1800
ggtagattaa tcaagacttt agtctgtata cctactacat caggtaccgg tgcagaagtt 1860
accccatttg cagttgtcac atccgaagaa ggtagaaaat atcctttagt cgattatgaa 1920
ttaacccctg acatggctat tgttgatcca gaatttgccg ttggtatgcc aaagagattg 1980
acatcatgga ctggtatcga cgcattgact cacgcaatcg aatcctatgt cagtattatg 2040
gcaactgact ttacaagacc ttattcattg agagcagttg gtttaatttt tgaatccttg 2100
tcattagcct ataacaatgg taaagacatt gaagctagag aaaagatgca caatgcttcc 2160
gccattgccg gtatggcctt tgcaaatgca tttttgggtt gctgtcattc cgtagcccac 2220
caattgggtt ctgtttatca catccctcat ggtttggcca atgctttgat gttgtcccac 2280
ataatcaaat ataatgcaac cgactctcct gttaaaatgg gtacatttcc acaatacaaa 2340
taccctcaag caatgagaca ctatgctgaa atcgccgaat tattgttgcc acctacccaa 2400
gtcgtaaaaa tgactgatgt tgacaaagtt caatatttga ttgatagagt tgaacaattg 2460
aaagctgatg ttggtattcc taaatccatt aaggaaactg gtatggtcac cgaagaagat 2520
ttctttaaca aggttgatca agttgctatc atggccttcg acgatcaatg tacaggtgct 2580
aacccaagat acccattagt ctccgaatta aaacaattga tgatagatgc ctggaatggt 2640
gtagtcccta agttataa 2658
<210> 25
<211> 1380
<212> DNA
<213> EUTEdz
<400> 25
atggaacact ccgtcattga accaaccgtc cctatgcctt tgccagctat gtttgatgcc 60
ccttcaggta tcttctcctc cttagacgat gcagttcaag ccgctacttt agcccaacaa 120
caattgtcat ccgttgaatt gagacaacaa gtcataaagg caattagagt tgccggtgaa 180
agatacgctc aagtcttggc agaaatggcc gtcgcagaaa ccggtatggg tagagttgta 240
gataaataca ttaagaatgt ttcacaagca agacatacac caggtatcga atgtttatcc 300
gctgaagtat taacaggtga caacggtttg accttgattg aaaatgcacc ttggggtgtt 360
gttgcttccg ttacaccatc aaccaaccca gctgctacag ttattaacaa cgcaatttct 420
atgatcgctg ccggtaactc agttgtcttt gcacctcatc catctgcaaa gaatgtttcc 480
ttaagaacta tctccttgtt gaataaggca atagtagcca caggtggtcc agaaaatttg 540
ttagtctctg ttgcaaaccc taatatcgaa actgcacaaa gattgtttag atatccaggt 600
atcggtttgt tggttgtaac cggtggtgaa gctgtagtcg aagctgcaag aaagcatacc 660
gataaaagat taatcgctgc aggtgcaggt aatccaccag ttgttgttga cgaaacagct 720
gatatcccta aggcagcaag agccattgtt aaaggtgcat cattcgataa caatattatc 780
tgtgccgatg aaaaagtttt aattgtagtt gacagagttg ccgatgcatt attggccgaa 840
atgcaaagaa ataacgcagt attattgact ccagaacaaa cagaaagatt gttaccagct 900
ttgttatctg atatcgacga acaaggtaaa ggtagagtta acagagacta cgttggtaga 960
gatgccgcta aattagcagc tgcaatcggt ttggaagtct cagaacatac aagattattg 1020
ttggcagaaa cagatgcaga tcatccattc gctgttacag aattaatgat gccagtttta 1080
cctgtcatca gagtcaagaa cgttgatgac gcaattgctt tggcagtcaa attggaatct 1140
ggttgtagac atactgctgc tatgcattca accaacataa gaaatttgaa tagaatggcc 1200
aacgctataa atacatcaat attcgttaag aatggtcctt gtatcgctgg tttaggtttg 1260
ggtggtgaag gttggacaag tatgacaatc agtaccccaa ctggtgaagg tgttacatca 1320
gctagaacct ttgttagatt gagaagatgc gtcttagtag atatgtttag aatagcctaa 1380
<210> 26
<211> 1404
<212> DNA
<213> EUTEec
<400> 26
atgaaccaac aagacataga acaagtagta aaagccgtat tattgaagat gcaaagttcc 60
gacactccac cagccgccgt ccacgaaatg ggtgtattcg catcattaga tgacgcagtt 120
gctgccgcta aagttgccca acaaggtttg aagtcagttg ctatgagaca attggcaatt 180
gctgcaatta gagaagctgg tgaaaaacat gccagagact tggccgaatt agctgtctca 240
gaaactggta tgggtagagt tgaagataaa tttgccaaaa acgttgcaca agctagaggt 300
actcctggtg tagaatgttt gtccccacaa gttttaacag gtgacaacgg tttgacctta 360
attgaaaatg ccccttgggg tgttgtagct tctgtcacac cttcaaccaa ccctgctgct 420
accttgatca ataacgctat ttccttgatt gctgccggta attccgttat cttcgctcca 480
catccagcag ccaagaaagt ttcacaaaga gccatcacct tgttgaatca agcaatcgtt 540
gctgcaggtg gtccagaaaa cttattggtt acagtcgcaa accctgacat cgaaacagct 600
caaagattgt ttaaatttcc tggtataggt ttgttagttg taactggtgg tgaagctgta 660
gttgaagcag ctagaaagca tactaataag agattgattg ctgcaggtgc cggtaatcct 720
ccagtcgttg ttgatgaaac agcagatttg gccagagctg cacaatccat cgttaagggt 780
gcatctttcg ataacaatat tatctgtgca gatgaaaagg tcttaatcgt tgtagattcc 840
gttgctgatg aattgatgag attgatggaa ggtcaacatg cagtcaaatt gacagctgaa 900
caagcccaac aattgcaacc tgtattattg aagaatatcg acgaaagagg taaaggtacc 960
gtttcaagag actgggttgg tagagatgct gcaaaaattg ctgcagctat cggtttaaac 1020
gttcctcaag aaaccagatt gttgtttgtt gaaactaccg cagaacaccc tttcgcagtt 1080
accgaattga tgatgcctgt attgccagta gtcagagttg ccaacgttgc tgatgcaata 1140
gccttggcag ttaaattgga aggtggttgt caccatacag cagctatgca ttcaagaaat 1200
atagaaaata tgaaccaaat ggctaacgca atagacacat ctatctttgt taagaacggt 1260
ccttgcatag ctggtttggg tttaggtggt gaaggttgga caaccatgac aatcactact 1320
ccaacaggtg aaggtgtcac atcagcaaga acctttgtta gattaagaag atgcgtcttg 1380
gtagatgcct ttagaatagt ataa 1404
<210> 27
<211> 1410
<212> DNA
<213> LIN1129li
<400> 27
atggaatcat tagaattaga acaattagtc aagaaggtat tattagaaaa gttggcagaa 60
caaaaggaag tccctacaaa gaccacaacc caaggtgcaa aatctggtgt attcgataca 120
gttgacgaag ccgtacaagc agctgttata gctcaaaatt gttacaagga aaaatccttg 180
gaagaaagaa gaaatgttgt aaaagcaata agagaagcct tatacccaga aattgaaact 240
atagctacaa gagcagttgc tgaaactggt atgggtaacg ttaccgataa aattttgaag 300
aataccttgg ctattgaaaa gacccctggt gtagaagatt tgtacacaga agtcgctaca 360
ggtgacaatg gtatgacctt gtatgaatta tcaccatacg gtgttattgg tgctgttgca 420
ccttctacca acccaaccga aacattaatt tgtaactcta ttggtatgtt agcagctggt 480
aatgccgtat tttattcacc acaccctggt gccaaaaata tctcattatg gttgattgaa 540
aagttgaata ccatagttag agattcctgt ggtatagata acttgatagt aacagtcgct 600
aaaccaagta tccaagctgc acaagaaatg atgaatcatc ctaaggttcc attattggtt 660
ataaccggtg gtccaggtgt tgtcttgcaa gcaatgcaat ccggtaaaaa ggttatcggt 720
gcaggtgcag gtaatcctcc aagtatagtt gacgaaactg ctaacataga aaaagcagcc 780
gcagacatcg ttgatggtgc ctcatttgat cataatatct tgtgtatagc tgaaaagagt 840
gtcgttgctg tagattccat tgccgatttc ttgttatttc aaatggaaaa gaatggtgca 900
ttgcatgtta ccaacccttc tgatattcaa aaattagaaa aggtagccgt tacagataag 960
ggtgtcacta ataagaaatt agttggtaaa tccgccactg aaatcttgaa ggaagcaggt 1020
atagcttgtg actttacacc aagattgata atcgttgaaa ccgaaaaatc ccatccattt 1080
gcaacagttg aattgttaat gcctatcgtt ccagttgtca gagttccaga tttcgatgaa 1140
gcattggaag ttgctattga attagaacaa ggtttgcatc acacagctac tatgcactct 1200
caaaatattt ccagattgaa taaggcagca agagatatgc aaacaagtat cttcgtcaaa 1260
aatggtcctt cttttgcagg tttgggtttc agaggtgaag gttcaaccac ttttacaatt 1320
gccactccaa ccggtgaagg tactaccaca gccagacatt tcgccagaag aagaagatgc 1380
gtcttgacag atggttttag tattagataa 1410
<210> 28
<211> 6702
<212> DNA
<213> ACC1sc
<400> 28
atgagcgaag aaagcttatt cgagtcttct ccacagaaga tggagtacga aattacaaac 60
tactcagaaa gacatacaga acttccaggt catttcattg gcctcaatac agtagataaa 120
ctagaggagt ccccgttaag ggactttgtt aagagtcacg gtggtcacac ggtcatatcc 180
aagatcctga tagcaaataa tggtattgcc gccgtgaaag aaattagatc cgtcagaaaa 240
tgggcatacg agacgttcgg cgatgacaga accgtccaat tcgtcgccat ggccacccca 300
gaagatctgg aggccaacgc agaatatatc cgtatggccg atcaatacat tgaagtgcca 360
ggtggtacta ataataacaa ctacgctaac gtagacttga tcgtagacat cgccgaaaga 420
gcagacgtag acgccgtatg ggctggctgg ggtcacgcct ccgagaatcc actattgcct 480
gaaaaattgt cccagtctaa gaggaaagtc atctttattg ggcctccagg taacgccatg 540
aggtctttag gtgataaaat ctcctctacc attgtcgctc aaagtgctaa agtcccatgt 600
attccatggt ctggtaccgg tgttgacacc gttcacgtgg acgagaaaac cggtctggtc 660
tctgtcgacg atgacatcta tcaaaagggt tgttgtacct ctcctgaaga tggtttacaa 720
aaggccaagc gtattggttt tcctgtcatg attaaggcat ccgaaggtgg tggtggtaaa 780
ggtatcagac aagttgaacg tgaagaagat ttcatcgctt tataccacca ggcagccaac 840
gaaattccag gctcccccat tttcatcatg aagttggccg gtagagcgcg tcacttggaa 900
gttcaactgc tagcagatca gtacggtaca aatatttcct tgttcggtag agactgttcc 960
gttcagagac gtcatcaaaa aattatcgaa gaagcaccag ttacaattgc caaggctgaa 1020
acatttcacg agatggaaaa ggctgccgtc agactgggga aactagtcgg ttatgtctct 1080
gccggtaccg tggagtatct atattctcat gatgatggaa aattctactt tttagaattg 1140
aacccaagat tacaagtcga gcatccaaca acggaaatgg tctccggtgt taacttacct 1200
gcagctcaat tacaaatcgc tatgggtatc cctatgcata gaataagtga cattagaact 1260
ttatatggta tgaatcctca ttctgcctca gaaatcgatt tcgaattcaa aactcaagat 1320
gccaccaaga aacaaagaag acctattcca aagggtcatt gtaccgcttg tcgtatcaca 1380
tcagaagatc caaacgatgg attcaagcca tcgggtggta ctttgcatga actaaacttc 1440
cgttcttcct ctaatgtttg gggttacttc tccgtgggta acaatggtaa tattcactcc 1500
ttttcggact ctcagttcgg ccatattttt gcttttggtg aaaatagaca agcttccagg 1560
aaacacatgg ttgttgccct gaaggaattg tccattaggg gtgatttcag aactactgtg 1620
gaatacttga tcaaactttt ggaaactgaa gatttcgagg ataacactat taccaccggt 1680
tggttggacg atttgattac tcataaaatg accgctgaaa agcctgatcc aactcttgcc 1740
gtcatttgcg gtgccgctac aaaggctttc ttagcatctg aagaagcccg ccacaagtat 1800
atcgaatcct tacaaaaggg acaagttcta tctaaagacc tactgcaaac tatgttccct 1860
gtagatttta tccatgaggg taaaagatac aagttcaccg tagctaaatc cggtaatgac 1920
cgttacacat tatttatcaa tggttctaaa tgtgatatca tactgcgtca actagctgat 1980
ggtggtcttt tgattgccat aggcggtaaa tcgcatacca tctattggaa agaagaagtt 2040
gctgctacaa gattatccgt tgactctatg actactttgt tggaagttga aaacgatcca 2100
acccagttgc gtactccatc ccctggtaaa ttggttaaat tcttggtgga aaatggtgaa 2160
cacattatca agggccaacc atatgcagaa attgaagtta tgaaaatgca aatgcctttg 2220
gtttctcaag aaaatggtat cgtccagtta ttaaagcaac ctggttctac cattgttgca 2280
ggtgatatca tggctattat gactcttgac gatccatcca aggtcaagca cgctctacca 2340
tttgaaggta tgctgccaga ttttggttct ccagttatcg aaggaaccaa acctgcctat 2400
aaattcaagt cattagtgtc tactttggaa aacattttga agggttatga caaccaagtt 2460
attatgaacg cttccttgca acaattgata gaggttttga gaaatccaaa actgccttac 2520
tcagaatgga aactacacat ctctgcttta cattcaagat tgcctgctaa gctagatgaa 2580
caaatggaag agttagttgc acgttctttg agacgtggtg ctgttttccc agctagacaa 2640
ttaagtaaat tgattgatat ggccgtgaag aatcctgaat acaaccccga caaattgctg 2700
ggcgccgtcg tggaaccatt ggcggatatt gctcataagt actctaacgg gttagaagcc 2760
catgaacatt ctatatttgt ccatttcttg gaagaatatt acgaagttga aaagttattc 2820
aatggtccaa atgttcgtga ggaaaatatc attctgaaat tgcgtgatga aaaccctaaa 2880
gatctagata aagttgcgct aactgttttg tctcattcga aagtttcagc gaagaataac 2940
ctgatcctag ctatcttgaa acattatcaa ccattgtgca agttatcttc taaagtttct 3000
gccattttct ctactcctct acaacatatt gttgaactag aatctaaggc taccgctaag 3060
gtcgctctac aagcaagaga aattttgatt caaggcgctt taccttcggt caaggaaaga 3120
actgaacaaa ttgaacatat cttaaaatcc tctgttgtga aggttgccta tggctcatcc 3180
aatccaaagc gctctgaacc agatttgaat atcttgaagg acttgatcga ttctaattac 3240
gttgtgttcg atgttttact tcaattccta acccatcaag acccagttgt gactgctgca 3300
gctgctcaag tctatattcg tcgtgcttat cgtgcttaca ccataggaga tattagagtt 3360
cacgaaggtg tcacagttcc aattgttgaa tggaaattcc aactaccttc agctgcgttc 3420
tccacctttc caactgttaa atctaaaatg ggtatgaaca gggctgtttc tgtttcagat 3480
ttgtcatatg ttgcaaacag tcagtcatct ccgttaagag aaggtatttt gatggctgtg 3540
gatcatttag atgatgttga tgaaattttg tcacaaagtt tggaagttat tcctcgtcac 3600
caatcttctt ctaacggacc tgctcctgat cgttctggta gctccgcatc gttgagtaat 3660
gttgctaatg tttgtgttgc ttctacagaa ggtttcgaat ctgaagagga aattttggta 3720
aggttgagag aaattttgga tttgaataag caggaattaa tcaatgcttc tatccgtcgt 3780
atcacattta tgttcggttt taaagatggg tcttatccaa agtattatac ttttaacggt 3840
ccaaattata acgaaaatga aacaattcgt cacattgagc cggctttggc cttccaactg 3900
gaattaggaa gattgtccaa cttcaacatt aaaccaattt tcactgataa tagaaacatc 3960
catgtctacg aagctgttag taagacttct ccattggata agagattctt tacaagaggt 4020
attattagaa cgggtcatat ccgtgatgac atttctattc aagaatatct gacttctgaa 4080
gctaacagat tgatgagtga tatattggat aatttagaag tcaccgacac ttcaaattct 4140
gatttgaatc atatcttcat caacttcatt gcggtgtttg atatctctcc agaagatgtc 4200
gaagccgcct tcggtggttt cttagaaaga tttggtaaga gattgttgag attgcgtgtt 4260
tcttctgccg aaattagaat catcatcaaa gatcctcaaa caggtgcccc agtaccattg 4320
cgtgccttga tcaataacgt ttctggttat gttatcaaaa cagaaatgta caccgaagtc 4380
aagaacgcaa aaggtgaatg ggtatttaag tctttgggta aacctggatc catgcattta 4440
agacctattg ctactcctta ccctgttaag gaatggttgc aaccaaaacg ttataaggca 4500
cacttgatgg gtaccacata tgtctatgac ttcccagaat tattccgcca agcatcgtca 4560
tcccaatgga aaaatttctc tgcagatgtt aagttaacag atgatttctt tatttccaac 4620
gagttgattg aagatgaaaa cggcgaatta actgaggtgg aaagagaacc tggtgccaac 4680
gctattggta tggttgcctt taagattact gtaaagactc ctgaatatcc aagaggccgt 4740
caatttgttg ttgttgctaa cgatatcaca ttcaagatcg gttcctttgg tccacaagaa 4800
gacgaattct tcaataaggt tactgaatat gctagaaagc gtggtatccc aagaatttac 4860
ttggctgcaa actcaggtgc cagaattggt atggctgaag agattgttcc actatttcaa 4920
gttgcatgga atgatgctgc caatccggac aagggcttcc aatacttata cttaacaagt 4980
gaaggtatgg aaactttaaa gaaatttgac aaagaaaatt ctgttctcac tgaacgtact 5040
gttataaacg gtgaagaaag atttgtcatc aagacaatta ttggttctga agatgggtta 5100
ggtgtcgaat gtctacgtgg atctggttta attgctggtg caacgtcaag ggcttaccac 5160
gatatcttca ctatcacctt agtcacttgt agatccgtcg gtatcggtgc ttatttggtt 5220
cgtttgggtc aaagagctat tcaggtcgaa ggccagccaa ttattttaac tggtgctcct 5280
gcaatcaaca aaatgctggg tagagaagtt tatacttcta acttacaatt gggtggtact 5340
caaatcatgt ataacaacgg tgtttcacat ttgactgctg ttgacgattt agctggtgta 5400
gagaagattg ttgaatggat gtcttatgtt ccagccaagc gtaatatgcc agttcctatc 5460
ttggaaacta aagacacatg ggatagacca gttgatttca ctccaactaa tgatgaaact 5520
tacgatgtaa gatggatgat tgaaggtcgt gagactgaaa gtggatttga atatggtttg 5580
tttgataaag ggtctttctt tgaaactttg tcaggatggg ccaaaggtgt tgtcgttggt 5640
agagcccgtc ttggtggtat tccactgggt gttattggtg ttgaaacaag aactgtcgag 5700
aacttgattc ctgctgatcc agctaatcca aatagtgctg aaacattaat tcaagaacct 5760
ggtcaagttt ggcatccaaa ctccgccttc aagactgctc aagctatcaa tgactttaac 5820
aacggtgaac aattgccaat gatgattttg gccaactgga gaggtttctc tggtggtcaa 5880
cgtgatatgt tcaacgaagt cttgaagtat ggttcgttta ttgttgacgc attggtggat 5940
tacaaacaac caattattat ctatatccca cctaccggtg aactaagagg tggttcatgg 6000
gttgttgtcg atccaactat caacgctgac caaatggaaa tgtatgccga cgtcaacgct 6060
agagctggtg ttttggaacc acaaggtatg gttggtatca agttccgtag agaaaaattg 6120
ctggacacca tgaacagatt ggatgacaag tacagagaat tgagatctca attatccaac 6180
aagagtttgg ctccagaagt acatcagcaa atatccaagc aattagctga tcgtgagaga 6240
gaactattgc caatttacgg acaaatcagt cttcaatttg ctgatttgca cgataggtct 6300
tcacgtatgg tggccaaggg tgttatttct aaggaactgg aatggaccga ggcacgtcgt 6360
ttcttcttct ggagattgag aagaagattg aacgaagaat atttgattaa aaggttgagc 6420
catcaggtag gcgaagcatc aagattagaa aagatcgcaa gaattagatc gtggtaccct 6480
gcttcagtgg accatgaaga tgataggcaa gtcgcaacat ggattgaaga aaactacaaa 6540
actttggacg ataaactaaa gggtttgaaa ttagagtcat tcgctcaaga cttagctaaa 6600
aagatcagaa gcgaccatga caatgctatt gatggattat ctgaagttat caagatgtta 6660
tctaccgatg ataaagaaaa attgttgaag actttgaaat aa 6702
<210> 29
<211> 6702
<212> DNA
<213> ACC1sc_S659A/S1157A
<400> 29
atgagcgaag aaagcttatt cgagtcttct ccacagaaga tggagtacga aattacaaac 60
tactcagaaa gacatacaga acttccaggt catttcattg gcctcaatac agtagataaa 120
ctagaggagt ccccgttaag ggactttgtt aagagtcacg gtggtcacac ggtcatatcc 180
aagatcctga tagcaaataa tggtattgcc gccgtgaaag aaattagatc cgtcagaaaa 240
tgggcatacg agacgttcgg cgatgacaga accgtccaat tcgtcgccat ggccacccca 300
gaagatctgg aggccaacgc agaatatatc cgtatggccg atcaatacat tgaagtgcca 360
ggtggtacta ataataacaa ctacgctaac gtagacttga tcgtagacat cgccgaaaga 420
gcagacgtag acgccgtatg ggctggctgg ggtcacgcct ccgagaatcc actattgcct 480
gaaaaattgt cccagtctaa gaggaaagtc atctttattg ggcctccagg taacgccatg 540
aggtctttag gtgataaaat ctcctctacc attgtcgctc aaagtgctaa agtcccatgt 600
attccatggt ctggtaccgg tgttgacacc gttcacgtgg acgagaaaac cggtctggtc 660
tctgtcgacg atgacatcta tcaaaagggt tgttgtacct ctcctgaaga tggtttacaa 720
aaggccaagc gtattggttt tcctgtcatg attaaggcat ccgaaggtgg tggtggtaaa 780
ggtatcagac aagttgaacg tgaagaagat ttcatcgctt tataccacca ggcagccaac 840
gaaattccag gctcccccat tttcatcatg aagttggccg gtagagcgcg tcacttggaa 900
gttcaactgc tagcagatca gtacggtaca aatatttcct tgttcggtag agactgttcc 960
gttcagagac gtcatcaaaa aattatcgaa gaagcaccag ttacaattgc caaggctgaa 1020
acatttcacg agatggaaaa ggctgccgtc agactgggga aactagtcgg ttatgtctct 1080
gccggtaccg tggagtatct atattctcat gatgatggaa aattctactt tttagaattg 1140
aacccaagat tacaagtcga gcatccaaca acggaaatgg tctccggtgt taacttacct 1200
gcagctcaat tacaaatcgc tatgggtatc cctatgcata gaataagtga cattagaact 1260
ttatatggta tgaatcctca ttctgcctca gaaatcgatt tcgaattcaa aactcaagat 1320
gccaccaaga aacaaagaag acctattcca aagggtcatt gtaccgcttg tcgtatcaca 1380
tcagaagatc caaacgatgg attcaagcca tcgggtggta ctttgcatga actaaacttc 1440
cgttcttcct ctaatgtttg gggttacttc tccgtgggta acaatggtaa tattcactcc 1500
ttttcggact ctcagttcgg ccatattttt gcttttggtg aaaatagaca agcttccagg 1560
aaacacatgg ttgttgccct gaaggaattg tccattaggg gtgatttcag aactactgtg 1620
gaatacttga tcaaactttt ggaaactgaa gatttcgagg ataacactat taccaccggt 1680
tggttggacg atttgattac tcataaaatg accgctgaaa agcctgatcc aactcttgcc 1740
gtcatttgcg gtgccgctac aaaggctttc ttagcatctg aagaagcccg ccacaagtat 1800
atcgaatcct tacaaaaggg acaagttcta tctaaagacc tactgcaaac tatgttccct 1860
gtagatttta tccatgaggg taaaagatac aagttcaccg tagctaaatc cggtaatgac 1920
cgttacacat tatttatcaa tggttctaaa tgtgatatca tactgcgtca actagctgat 1980
ggtggtcttt tgattgccat aggcggtaaa tcgcatacca tctattggaa agaagaagtt 2040
gctgctacaa gattatccgt tgactctatg actactttgt tggaagttga aaacgatcca 2100
acccagttgc gtactccatc ccctggtaaa ttggttaaat tcttggtgga aaatggtgaa 2160
cacattatca agggccaacc atatgcagaa attgaagtta tgaaaatgca aatgcctttg 2220
gtttctcaag aaaatggtat cgtccagtta ttaaagcaac ctggttctac cattgttgca 2280
ggtgatatca tggctattat gactcttgac gatccatcca aggtcaagca cgctctacca 2340
tttgaaggta tgctgccaga ttttggttct ccagttatcg aaggaaccaa acctgcctat 2400
aaattcaagt cattagtgtc tactttggaa aacattttga agggttatga caaccaagtt 2460
attatgaacg cttccttgca acaattgata gaggttttga gaaatccaaa actgccttac 2520
tcagaatgga aactacacat ctctgcttta cattcaagat tgcctgctaa gctagatgaa 2580
caaatggaag agttagttgc acgttctttg agacgtggtg ctgttttccc agctagacaa 2640
ttaagtaaat tgattgatat ggccgtgaag aatcctgaat acaaccccga caaattgctg 2700
ggcgccgtcg tggaaccatt ggcggatatt gctcataagt actctaacgg gttagaagcc 2760
catgaacatt ctatatttgt ccatttcttg gaagaatatt acgaagttga aaagttattc 2820
aatggtccaa atgttcgtga ggaaaatatc attctgaaat tgcgtgatga aaaccctaaa 2880
gatctagata aagttgcgct aactgttttg tctcattcga aagtttcagc gaagaataac 2940
ctgatcctag ctatcttgaa acattatcaa ccattgtgca agttatcttc taaagtttct 3000
gccattttct ctactcctct acaacatatt gttgaactag aatctaaggc taccgctaag 3060
gtcgctctac aagcaagaga aattttgatt caaggcgctt taccttcggt caaggaaaga 3120
actgaacaaa ttgaacatat cttaaaatcc tctgttgtga aggttgccta tggctcatcc 3180
aatccaaagc gctctgaacc agatttgaat atcttgaagg acttgatcga ttctaattac 3240
gttgtgttcg atgttttact tcaattccta acccatcaag acccagttgt gactgctgca 3300
gctgctcaag tctatattcg tcgtgcttat cgtgcttaca ccataggaga tattagagtt 3360
cacgaaggtg tcacagttcc aattgttgaa tggaaattcc aactaccttc agctgcgttc 3420
tccacctttc caactgttaa atctaaaatg ggtatgaaca gggctgttgc tgtttcagat 3480
ttgtcatatg ttgcaaacag tcagtcatct ccgttaagag aaggtatttt gatggctgtg 3540
gatcatttag atgatgttga tgaaattttg tcacaaagtt tggaagttat tcctcgtcac 3600
caatcttctt ctaacggacc tgctcctgat cgttctggta gctccgcatc gttgagtaat 3660
gttgctaatg tttgtgttgc ttctacagaa ggtttcgaat ctgaagagga aattttggta 3720
aggttgagag aaattttgga tttgaataag caggaattaa tcaatgcttc tatccgtcgt 3780
atcacattta tgttcggttt taaagatggg tcttatccaa agtattatac ttttaacggt 3840
ccaaattata acgaaaatga aacaattcgt cacattgagc cggctttggc cttccaactg 3900
gaattaggaa gattgtccaa cttcaacatt aaaccaattt tcactgataa tagaaacatc 3960
catgtctacg aagctgttag taagacttct ccattggata agagattctt tacaagaggt 4020
attattagaa cgggtcatat ccgtgatgac atttctattc aagaatatct gacttctgaa 4080
gctaacagat tgatgagtga tatattggat aatttagaag tcaccgacac ttcaaattct 4140
gatttgaatc atatcttcat caacttcatt gcggtgtttg atatctctcc agaagatgtc 4200
gaagccgcct tcggtggttt cttagaaaga tttggtaaga gattgttgag attgcgtgtt 4260
tcttctgccg aaattagaat catcatcaaa gatcctcaaa caggtgcccc agtaccattg 4320
cgtgccttga tcaataacgt ttctggttat gttatcaaaa cagaaatgta caccgaagtc 4380
aagaacgcaa aaggtgaatg ggtatttaag tctttgggta aacctggatc catgcattta 4440
agacctattg ctactcctta ccctgttaag gaatggttgc aaccaaaacg ttataaggca 4500
cacttgatgg gtaccacata tgtctatgac ttcccagaat tattccgcca agcatcgtca 4560
tcccaatgga aaaatttctc tgcagatgtt aagttaacag atgatttctt tatttccaac 4620
gagttgattg aagatgaaaa cggcgaatta actgaggtgg aaagagaacc tggtgccaac 4680
gctattggta tggttgcctt taagattact gtaaagactc ctgaatatcc aagaggccgt 4740
caatttgttg ttgttgctaa cgatatcaca ttcaagatcg gttcctttgg tccacaagaa 4800
gacgaattct tcaataaggt tactgaatat gctagaaagc gtggtatccc aagaatttac 4860
ttggctgcaa actcaggtgc cagaattggt atggctgaag agattgttcc actatttcaa 4920
gttgcatgga atgatgctgc caatccggac aagggcttcc aatacttata cttaacaagt 4980
gaaggtatgg aaactttaaa gaaatttgac aaagaaaatt ctgttctcac tgaacgtact 5040
gttataaacg gtgaagaaag atttgtcatc aagacaatta ttggttctga agatgggtta 5100
ggtgtcgaat gtctacgtgg atctggttta attgctggtg caacgtcaag ggcttaccac 5160
gatatcttca ctatcacctt agtcacttgt agatccgtcg gtatcggtgc ttatttggtt 5220
cgtttgggtc aaagagctat tcaggtcgaa ggccagccaa ttattttaac tggtgctcct 5280
gcaatcaaca aaatgctggg tagagaagtt tatacttcta acttacaatt gggtggtact 5340
caaatcatgt ataacaacgg tgtttcacat ttgactgctg ttgacgattt agctggtgta 5400
gagaagattg ttgaatggat gtcttatgtt ccagccaagc gtaatatgcc agttcctatc 5460
ttggaaacta aagacacatg ggatagacca gttgatttca ctccaactaa tgatgaaact 5520
tacgatgtaa gatggatgat tgaaggtcgt gagactgaaa gtggatttga atatggtttg 5580
tttgataaag ggtctttctt tgaaactttg tcaggatggg ccaaaggtgt tgtcgttggt 5640
agagcccgtc ttggtggtat tccactgggt gttattggtg ttgaaacaag aactgtcgag 5700
aacttgattc ctgctgatcc agctaatcca aatagtgctg aaacattaat tcaagaacct 5760
ggtcaagttt ggcatccaaa ctccgccttc aagactgctc aagctatcaa tgactttaac 5820
aacggtgaac aattgccaat gatgattttg gccaactgga gaggtttctc tggtggtcaa 5880
cgtgatatgt tcaacgaagt cttgaagtat ggttcgttta ttgttgacgc attggtggat 5940
tacaaacaac caattattat ctatatccca cctaccggtg aactaagagg tggttcatgg 6000
gttgttgtcg atccaactat caacgctgac caaatggaaa tgtatgccga cgtcaacgct 6060
agagctggtg ttttggaacc acaaggtatg gttggtatca agttccgtag agaaaaattg 6120
ctggacacca tgaacagatt ggatgacaag tacagagaat tgagatctca attatccaac 6180
aagagtttgg ctccagaagt acatcagcaa atatccaagc aattagctga tcgtgagaga 6240
gaactattgc caatttacgg acaaatcagt cttcaatttg ctgatttgca cgataggtct 6300
tcacgtatgg tggccaaggg tgttatttct aaggaactgg aatggaccga ggcacgtcgt 6360
ttcttcttct ggagattgag aagaagattg aacgaagaat atttgattaa aaggttgagc 6420
catcaggtag gcgaagcatc aagattagaa aagatcgcaa gaattagatc gtggtaccct 6480
gcttcagtgg accatgaaga tgataggcaa gtcgcaacat ggattgaaga aaactacaaa 6540
actttggacg ataaactaaa gggtttgaaa ttagagtcat tcgctcaaga cttagctaaa 6600
aagatcagaa gcgaccatga caatgctatt gatggattat ctgaagttat caagatgtta 6660
tctaccgatg ataaagaaaa attgttgaag actttgaaat aa 6702
<210> 30
<211> 6702
<212> DNA
<213> ACC1sc_S1157A
<400> 30
atgagcgaag aaagcttatt cgagtcttct ccacagaaga tggagtacga aattacaaac 60
tactcagaaa gacatacaga acttccaggt catttcattg gcctcaatac agtagataaa 120
ctagaggagt ccccgttaag ggactttgtt aagagtcacg gtggtcacac ggtcatatcc 180
aagatcctga tagcaaataa tggtattgcc gccgtgaaag aaattagatc cgtcagaaaa 240
tgggcatacg agacgttcgg cgatgacaga accgtccaat tcgtcgccat ggccacccca 300
gaagatctgg aggccaacgc agaatatatc cgtatggccg atcaatacat tgaagtgcca 360
ggtggtacta ataataacaa ctacgctaac gtagacttga tcgtagacat cgccgaaaga 420
gcagacgtag acgccgtatg ggctggctgg ggtcacgcct ccgagaatcc actattgcct 480
gaaaaattgt cccagtctaa gaggaaagtc atctttattg ggcctccagg taacgccatg 540
aggtctttag gtgataaaat ctcctctacc attgtcgctc aaagtgctaa agtcccatgt 600
attccatggt ctggtaccgg tgttgacacc gttcacgtgg acgagaaaac cggtctggtc 660
tctgtcgacg atgacatcta tcaaaagggt tgttgtacct ctcctgaaga tggtttacaa 720
aaggccaagc gtattggttt tcctgtcatg attaaggcat ccgaaggtgg tggtggtaaa 780
ggtatcagac aagttgaacg tgaagaagat ttcatcgctt tataccacca ggcagccaac 840
gaaattccag gctcccccat tttcatcatg aagttggccg gtagagcgcg tcacttggaa 900
gttcaactgc tagcagatca gtacggtaca aatatttcct tgttcggtag agactgttcc 960
gttcagagac gtcatcaaaa aattatcgaa gaagcaccag ttacaattgc caaggctgaa 1020
acatttcacg agatggaaaa ggctgccgtc agactgggga aactagtcgg ttatgtctct 1080
gccggtaccg tggagtatct atattctcat gatgatggaa aattctactt tttagaattg 1140
aacccaagat tacaagtcga gcatccaaca acggaaatgg tctccggtgt taacttacct 1200
gcagctcaat tacaaatcgc tatgggtatc cctatgcata gaataagtga cattagaact 1260
ttatatggta tgaatcctca ttctgcctca gaaatcgatt tcgaattcaa aactcaagat 1320
gccaccaaga aacaaagaag acctattcca aagggtcatt gtaccgcttg tcgtatcaca 1380
tcagaagatc caaacgatgg attcaagcca tcgggtggta ctttgcatga actaaacttc 1440
cgttcttcct ctaatgtttg gggttacttc tccgtgggta acaatggtaa tattcactcc 1500
ttttcggact ctcagttcgg ccatattttt gcttttggtg aaaatagaca agcttccagg 1560
aaacacatgg ttgttgccct gaaggaattg tccattaggg gtgatttcag aactactgtg 1620
gaatacttga tcaaactttt ggaaactgaa gatttcgagg ataacactat taccaccggt 1680
tggttggacg atttgattac tcataaaatg accgctgaaa agcctgatcc aactcttgcc 1740
gtcatttgcg gtgccgctac aaaggctttc ttagcatctg aagaagcccg ccacaagtat 1800
atcgaatcct tacaaaaggg acaagttcta tctaaagacc tactgcaaac tatgttccct 1860
gtagatttta tccatgaggg taaaagatac aagttcaccg tagctaaatc cggtaatgac 1920
cgttacacat tatttatcaa tggttctaaa tgtgatatca tactgcgtca actatctgat 1980
ggtggtcttt tgattgccat aggcggtaaa tcgcatacca tctattggaa agaagaagtt 2040
gctgctacaa gattatccgt tgactctatg actactttgt tggaagttga aaacgatcca 2100
acccagttgc gtactccatc ccctggtaaa ttggttaaat tcttggtgga aaatggtgaa 2160
cacattatca agggccaacc atatgcagaa attgaagtta tgaaaatgca aatgcctttg 2220
gtttctcaag aaaatggtat cgtccagtta ttaaagcaac ctggttctac cattgttgca 2280
ggtgatatca tggctattat gactcttgac gatccatcca aggtcaagca cgctctacca 2340
tttgaaggta tgctgccaga ttttggttct ccagttatcg aaggaaccaa acctgcctat 2400
aaattcaagt cattagtgtc tactttggaa aacattttga agggttatga caaccaagtt 2460
attatgaacg cttccttgca acaattgata gaggttttga gaaatccaaa actgccttac 2520
tcagaatgga aactacacat ctctgcttta cattcaagat tgcctgctaa gctagatgaa 2580
caaatggaag agttagttgc acgttctttg agacgtggtg ctgttttccc agctagacaa 2640
ttaagtaaat tgattgatat ggccgtgaag aatcctgaat acaaccccga caaattgctg 2700
ggcgccgtcg tggaaccatt ggcggatatt gctcataagt actctaacgg gttagaagcc 2760
catgaacatt ctatatttgt ccatttcttg gaagaatatt acgaagttga aaagttattc 2820
aatggtccaa atgttcgtga ggaaaatatc attctgaaat tgcgtgatga aaaccctaaa 2880
gatctagata aagttgcgct aactgttttg tctcattcga aagtttcagc gaagaataac 2940
ctgatcctag ctatcttgaa acattatcaa ccattgtgca agttatcttc taaagtttct 3000
gccattttct ctactcctct acaacatatt gttgaactag aatctaaggc taccgctaag 3060
gtcgctctac aagcaagaga aattttgatt caaggcgctt taccttcggt caaggaaaga 3120
actgaacaaa ttgaacatat cttaaaatcc tctgttgtga aggttgccta tggctcatcc 3180
aatccaaagc gctctgaacc agatttgaat atcttgaagg acttgatcga ttctaattac 3240
gttgtgttcg atgttttact tcaattccta acccatcaag acccagttgt gactgctgca 3300
gctgctcaag tctatattcg tcgtgcttat cgtgcttaca ccataggaga tattagagtt 3360
cacgaaggtg tcacagttcc aattgttgaa tggaaattcc aactaccttc agctgcgttc 3420
tccacctttc caactgttaa atctaaaatg ggtatgaaca gggctgttgc tgtttcagat 3480
ttgtcatatg ttgcaaacag tcagtcatct ccgttaagag aaggtatttt gatggctgtg 3540
gatcatttag atgatgttga tgaaattttg tcacaaagtt tggaagttat tcctcgtcac 3600
caatcttctt ctaacggacc tgctcctgat cgttctggta gctccgcatc gttgagtaat 3660
gttgctaatg tttgtgttgc ttctacagaa ggtttcgaat ctgaagagga aattttggta 3720
aggttgagag aaattttgga tttgaataag caggaattaa tcaatgcttc tatccgtcgt 3780
atcacattta tgttcggttt taaagatggg tcttatccaa agtattatac ttttaacggt 3840
ccaaattata acgaaaatga aacaattcgt cacattgagc cggctttggc cttccaactg 3900
gaattaggaa gattgtccaa cttcaacatt aaaccaattt tcactgataa tagaaacatc 3960
catgtctacg aagctgttag taagacttct ccattggata agagattctt tacaagaggt 4020
attattagaa cgggtcatat ccgtgatgac atttctattc aagaatatct gacttctgaa 4080
gctaacagat tgatgagtga tatattggat aatttagaag tcaccgacac ttcaaattct 4140
gatttgaatc atatcttcat caacttcatt gcggtgtttg atatctctcc agaagatgtc 4200
gaagccgcct tcggtggttt cttagaaaga tttggtaaga gattgttgag attgcgtgtt 4260
tcttctgccg aaattagaat catcatcaaa gatcctcaaa caggtgcccc agtaccattg 4320
cgtgccttga tcaataacgt ttctggttat gttatcaaaa cagaaatgta caccgaagtc 4380
aagaacgcaa aaggtgaatg ggtatttaag tctttgggta aacctggatc catgcattta 4440
agacctattg ctactcctta ccctgttaag gaatggttgc aaccaaaacg ttataaggca 4500
cacttgatgg gtaccacata tgtctatgac ttcccagaat tattccgcca agcatcgtca 4560
tcccaatgga aaaatttctc tgcagatgtt aagttaacag atgatttctt tatttccaac 4620
gagttgattg aagatgaaaa cggcgaatta actgaggtgg aaagagaacc tggtgccaac 4680
gctattggta tggttgcctt taagattact gtaaagactc ctgaatatcc aagaggccgt 4740
caatttgttg ttgttgctaa cgatatcaca ttcaagatcg gttcctttgg tccacaagaa 4800
gacgaattct tcaataaggt tactgaatat gctagaaagc gtggtatccc aagaatttac 4860
ttggctgcaa actcaggtgc cagaattggt atggctgaag agattgttcc actatttcaa 4920
gttgcatgga atgatgctgc caatccggac aagggcttcc aatacttata cttaacaagt 4980
gaaggtatgg aaactttaaa gaaatttgac aaagaaaatt ctgttctcac tgaacgtact 5040
gttataaacg gtgaagaaag atttgtcatc aagacaatta ttggttctga agatgggtta 5100
ggtgtcgaat gtctacgtgg atctggttta attgctggtg caacgtcaag ggcttaccac 5160
gatatcttca ctatcacctt agtcacttgt agatccgtcg gtatcggtgc ttatttggtt 5220
cgtttgggtc aaagagctat tcaggtcgaa ggccagccaa ttattttaac tggtgctcct 5280
gcaatcaaca aaatgctggg tagagaagtt tatacttcta acttacaatt gggtggtact 5340
caaatcatgt ataacaacgg tgtttcacat ttgactgctg ttgacgattt agctggtgta 5400
gagaagattg ttgaatggat gtcttatgtt ccagccaagc gtaatatgcc agttcctatc 5460
ttggaaacta aagacacatg ggatagacca gttgatttca ctccaactaa tgatgaaact 5520
tacgatgtaa gatggatgat tgaaggtcgt gagactgaaa gtggatttga atatggtttg 5580
tttgataaag ggtctttctt tgaaactttg tcaggatggg ccaaaggtgt tgtcgttggt 5640
agagcccgtc ttggtggtat tccactgggt gttattggtg ttgaaacaag aactgtcgag 5700
aacttgattc ctgctgatcc agctaatcca aatagtgctg aaacattaat tcaagaacct 5760
ggtcaagttt ggcatccaaa ctccgccttc aagactgctc aagctatcaa tgactttaac 5820
aacggtgaac aattgccaat gatgattttg gccaactgga gaggtttctc tggtggtcaa 5880
cgtgatatgt tcaacgaagt cttgaagtat ggttcgttta ttgttgacgc attggtggat 5940
tacaaacaac caattattat ctatatccca cctaccggtg aactaagagg tggttcatgg 6000
gttgttgtcg atccaactat caacgctgac caaatggaaa tgtatgccga cgtcaacgct 6060
agagctggtg ttttggaacc acaaggtatg gttggtatca agttccgtag agaaaaattg 6120
ctggacacca tgaacagatt ggatgacaag tacagagaat tgagatctca attatccaac 6180
aagagtttgg ctccagaagt acatcagcaa atatccaagc aattagctga tcgtgagaga 6240
gaactattgc caatttacgg acaaatcagt cttcaatttg ctgatttgca cgataggtct 6300
tcacgtatgg tggccaaggg tgttatttct aaggaactgg aatggaccga ggcacgtcgt 6360
ttcttcttct ggagattgag aagaagattg aacgaagaat atttgattaa aaggttgagc 6420
catcaggtag gcgaagcatc aagattagaa aagatcgcaa gaattagatc gtggtaccct 6480
gcttcagtgg accatgaaga tgataggcaa gtcgcaacat ggattgaaga aaactacaaa 6540
actttggacg ataaactaaa gggtttgaaa ttagagtcat tcgctcaaga cttagctaaa 6600
aagatcagaa gcgaccatga caatgctatt gatggattat ctgaagttat caagatgtta 6660
tctaccgatg ataaagaaaa attgttgaag actttgaaat aa 6702
<210> 31
<211> 6747
<212> DNA
<213> ACC1ke
<400> 31
atgagtgaag aaaatttatt tgaagattca tctgcaaaac gtcaatatga aataacagat 60
tactccaaga aacataaggc attggctcca cattttgtag ggttaaatac acttgacaaa 120
gtcgaagaat ctccactaaa agattttgtt aagtctcatg gtggtcatac tgttatctcc 180
aaaattttaa ttgctaataa tggtattgcc gcagttaaag aaattcgttc agttagaaaa 240
tggtcttatg aaacatttgg tgatgaaaga actgttcaat ttgttgcaat ggctactcca 300
gaagatttac aagctaattc tgaatatatt cgtatggctg atcaatacgt cgaagttcca 360
ggtggtacaa ataataacaa ttatgccaac gttgatctaa ttgttgatgt agcagaaaga 420
acagatgtcg atgcagtatg ggccggttgg ggtcatgctt ctgaaaatcc acatttacca 480
gaaaaattgg ctgcatcaaa gagaaagatt atattcattg gtcctccagg tagcgctatg 540
agatctttag gtgataaaat ttcttctaca attgttgctc aacatgccaa ggttccttgt 600
atcccatggt caggtactgg tattgataaa gttcacgttg acgaaacctc tggtttagtg 660
tctgttgatg atgatgttta ccaacaaggt tgttgccaat ctccagaaga tggtttagcc 720
aaagctaaac agattggttt cccagttatg atcaaagctt ccgaaggtgg tggtggtaaa 780
ggtatcagac aagttgaacg tgaagaagac tttattgcat tataccatca agctgctaat 840
gaaattccag gttctccaat ctttatcatg aagttagctg gcaaggctcg tcatttggaa 900
gttcaattat tggcagacca atatggtaca aatatttctc tatttggtcg tgattgttct 960
gttcaaagac gtcaccaaaa aattatcgaa gaagctccag ttacaattgc taacaatgaa 1020
acttttgaca agatggctaa cgctgccgtt agattaggta aattggttgg ttacgtatca 1080
gctggtacag tcgagtatct atattcaaat gaagagaaga aatactattt cttagaatta 1140
aacccaagat tacaagtgga gcatccaact acagaaatgg tctctggtgt taatttacca 1200
tctgcacaat tacaaattgc tatgggtata cctatgcaca gaattagcga tattagagtg 1260
ttatatggta tgaatccaca ttctgctact gaaatcgatt tcgaatttaa gactgaagaa 1320
gctaagaaga cacaaagaaa accagttcca aagggtcatt gtaccgcttg tcgtattaca 1380
tccgaagatc caaacgaagg attcaaacca tctggtggtt ctttacacga attgaatttc 1440
agatcctcat ctaatgtttg gggttatttc tctgtcggta acaacggtgg tatccattca 1500
ttctccgatt ctcagtttgg tcatatcttt gcctttggtg aaaacagaca aatgtcaaga 1560
aaacatatgg tggtagcatt aaaggaactc tctattagag gtgatttcag aactactgtt 1620
gaatatttga tcaaactttt ggaaaccgaa gattttgaag aaaatacaat tacaactggt 1680
tggttagatg atctaatttc tcataagatg actgcagaaa aacctgacgc tacgttagct 1740
gttatctgtg gtgctgctac aaaggctttt atcgcttctg aatctgctcg tatggaatac 1800
attgctcaat taaagagagg tcaagtccca gctaagaacc tattagagac tatgttccct 1860
gtcgaattca ttcatgaaaa taagagatac aaattcactg ttgctaaatc tgctgacgac 1920
tcttatactt tatttattaa tggttccaag tgtgaagtcc gtgccgttaa attatctgat 1980
ggtggtttgc taatatctgt tggtggtcaa tcccatacca tctattggaa ggaagaagct 2040
caagctacca gattatctat cgattctatg accactttac tagaagttga aaatgatcca 2100
acccaattac gtactccatc cccaggtaaa ttagttaaat tcttagttga aaatggcgat 2160
caaattaagg taggccaagc atatgccgaa attgaagtta tgaaaatgca aatgcctctg 2220
gtttctcaag aaaatggtgt aatacaatta ttgaaacaac ctggttctac tattgctgcc 2280
ggtgatatta ttgctatgct tgctctagat gatccatcca aggttaaaca tgctttacct 2340
tttgaaggta tgttaccaga attaggttcc ccaatggtcg aaggtactaa gcctgcttat 2400
aaattcaagt cattagtttc tactttagaa aatatattaa agggttacga taaccaagtc 2460
atcatgaatg catctctaca acaattaatt gaagttttaa gagatccaag attaccatac 2520
tctgaatgga aactatgtgt ttctgcatta cactccagat tgccaatgtc acttgatgaa 2580
aaactagatc aacttgttga ccgttctagt aatagaaatg ctatcttccc agctaaacaa 2640
ttaaacaaga tgattgaaga tgctgttaga gaacctggat ctgatgagtt actgccaaca 2700
atcgttgagc cactattaga tatcaccaat cgttatgcca atggtcttga agctcacgaa 2760
cattccgttt ttgtcaaatt tttggaagaa tactacaatg tcgaaaagtt gtttagttcc 2820
catggtaacc gtgaagaaaa tgtcattttg aaattacgtg atgaaaatct agattcttta 2880
gacaaggtcg tcgaaatagt cttatcgcat gctaaggtat ctgccaagaa cactttaatc 2940
ctagctattt tgaagcacta ccaaccaatt tgtaagatgt cttcttctgt tgctcatatt 3000
tttagtaaat cattaggtca catcgtggaa ttagaatcaa agtccgctgc caaggttgcg 3060
ttacaagcaa gagaaatctt aatccaaggt gctttaccat ctgttaaaga aagaaccgaa 3120
caaattgaac acatcctaag atcttctgtt acaaacacat cttatggtga tttggctcca 3180
aagaaatcag aaccaaactt agaaattcta aaggatttaa tcgattccaa ctatgttgtc 3240
tttgatgttc tgttaccatt tttatgccac gaagatccag tcatcgctac tgctgctgct 3300
caagtttacg tgcgtcgtgc ttacagagct tacactgttg gtgaagttaa gactccaaat 3360
attaaggatt catctattag tccattaatt gaatggaaat tccaattgcc ttctgctgca 3420
ttttcttcta ttccacaggt caagagcaaa attggtatga acagagctat gtctgtttct 3480
gatctttctt acgttgttga taatgaacat gctcctttaa gaacaggtat tttagttgcg 3540
tctgaacatt tagacgatgt cgatgcttgt ctagcccaag gtttggaagc tattccagaa 3600
catgctaata ctgctaatgg tccagctcca gatagatctg gtaattcatc ctctttgagt 3660
aatgttgcca atgtctgtgt ctcaagtact gaaggttttg aatctgaaaa agatatttta 3720
gcaagagttc acgaaatctt ggatttgaac aaatctgatt taatcaagtc ttctatccgt 3780
cgtattacat tcatgtttgg tttcgcagat ggtacatatc caaagtatta cacattcaac 3840
ggtccaaatt accaagaaaa tcaaactatt cgtcatatcg agccagcttg tgcattccaa 3900
ttggaattag gaagaatgtc taactttaat atcaagccaa tctttacaga aaatagaaat 3960
atccatgttt atgaagctat cagtaaaaca tctgcattag ataagagatt ctttaccaga 4020
ggtattatta gaactggtcg tatccgtgac gaaatttcaa ttcaagaata tcttacttct 4080
gaagctaaca agttaatgag tgacatttta gataatctag agattgttga cacatctaac 4140
tctgatttga accatatctt tatcaacttt tctgctgtct ttgatgtttc tccagaggat 4200
gttgaagctg cttttggtgg gttcttagaa agattcggga agagattctt aagacttcgt 4260
gtttcagctg ctgaaattcg tattattatt caagacccaa agacaggtgc ggctgttcca 4320
ttacgtgctt taatcaacaa tgtttctggt tacgtagtta aatccgagct atacactgaa 4380
attaagaatg ataagggaga atgggtcttt aagtcactag gtaaacctgg ttcaatgcat 4440
ttgagaccaa ttgctactcc atatccagtt aaggaatggt tacaacctaa acgttataag 4500
gctcatttaa tgggtaccac ttatctatac gatttcccag aattattcca tcaagctgcc 4560
attaccacat gggagaaata tctatctaac tcgtcagcta aggaattatc atctataaag 4620
gacagagacg atttatttat ttctcacgaa ttgatcgaag atgaaaatgg tgaattaact 4680
gaggtcgaaa gagaacctgg tgccaatact attggtatgg ttgcatttaa ggtcacaatg 4740
aagactccag aatatccaag aggtcgtcaa tttgtaattg ttgctaatga tatcactttc 4800
aagattggtt cctttggtcc acaagaagat gaatttttca acaaagttac tgaatatgct 4860
agaaagagag gtattccaag aatatacctg gctgctaact ctggtgccag aattggtatc 4920
gctgaagaat tggtgccatt attccaagtt gcttggaagg attctaaggt tccagcaaag 4980
ggtttcgaat atttatattt gagcaaagaa ggtatggaaa cattaaagag ttattctaag 5040
cagaattctg ttatcactga acgtgtcgtt gaagaaggtg aagaaagata tgttatcaaa 5100
tctattattg gtgctgatga tggtttaggt gttgaatgtt taagaggttc cggtttaatt 5160
gccggtgcta catcaagagc ttaccaagat attttcacaa ttactttagt tacatgtaga 5220
tctgtcggta ttggtgctta tttggttaga ttaggtcaaa gagccattca aattgaaggt 5280
caaccaatta ttttaactgg tgcaccagct atcaataaag tgttaggtag agaagtttat 5340
tcttctaatt tacaacttgg tggtactcaa attatgtaca ataatggtgt ttctcattta 5400
acagcttcag atgatttaca agctgttgaa caaatcatta attggttatc ttatgttccg 5460
gcaaagcgta atatgcctgt tccaatctta gaaaccgaag ataaatggga tagagccatt 5520
gatttcagac caactaccaa tgaacaatat gatgtcagat ggttaatcca aggtcgtgaa 5580
ttagacaacg gtgatttcga atacggtatg tttgataagg gttccttctt tgaaacttta 5640
tctggatggg ctaaaggtgt tgttgttggt agagctcgtt taggtggtat tccaatcggt 5700
gtcattggtg ttgaaacaag aaccattgaa aatgttattc cagctgatcc agcaaatcca 5760
gattctaccg aaactgttgt ccaagaagct ggtcaagttt ggtatccaaa ttctgcattt 5820
aagacggcac aagctattca agactttaac cacggtgaac aattaccatt aatgattcta 5880
gccaattgga gaggattctc tggtggtcaa cgtgatatgt acaatgaagt attgaaatat 5940
ggttcattta ttgttgatgc cttggttgat tacaaacaac ctatctttat ctatattcca 6000
ccaactggtg aattaagagg tggttcatgg gtcgtcgttg atccaactat taataatgat 6060
catatggaaa tgtatgctga taaagattcc cgtgctggtg tgttagaacc ttcaggtatg 6120
gttggtatta aataccgtag agagaaattg ttatctacta taacaagatt agattctacc 6180
tatagagaat tgaaagaaaa attatctgat aaattgttat ctactgaaga acatcaaaag 6240
atttctatac aactagctga acgtgaaaag atgttaattc caatctataa tcaaatcagt 6300
gtccaatttg ctgatctaca tgatagatca tctcgtatgt taaagaaagg tgttattaaa 6360
gctgaattga catgggaaga atctcgtcgt tatttcttct ggagattaag aagaagatta 6420
gatgaagaat acttattaag aagattagat tctcaattaa cccaagcttc aagattagag 6480
aagattgcac gtgttagatc ctggtatcca acttctgtcg atgtggagaa cgataaagaa 6540
gtagttacat ggattgaaga aaattatgaa acattagaag agaaattcaa agcccttaaa 6600
ttggaatcat ttgctttaga tttagctaaa aatattagaa atgatcatga aaatagtatg 6660
aagggtttag aagaagttgt tcgtatgtta agtgcaacag ataaagaaag attaaataag 6720
attattaatc cagatgataa aaaataa 6747
<210> 32
<211> 6651
<212> DNA
<213> ACC1mc
<400> 32
atggtcgaac ataagagctt gcctggccac tttttaggtg gtaattcact cgaatctgct 60
cctcagggtc ccgtcaaaga ctttgtccaa gcacatgagg gtcacacagt catttccaaa 120
gtcttgattg ccaacaacgg tatggctgca atgaaggaga tccgttccgt aagaaagtgg 180
gcttacgaga catttggtaa cgagagagct atcgaattca ctgtcatggc aacacccgag 240
gatttgaagg ccaatgctga atacatccgt atggccgata actttgtcga ggttcccggt 300
ggatccaaca acaacaacta tgccaatgtt gaactgattg tcgacgttgc cgaaagaact 360
gctgttcatg ctgtttgggc cggttggggt catgcttccg agaatcctcg tttgcctgaa 420
atgcttgcca agtccaagca caagtgtttg tttattggtc ctcctgcctc tgccatgaga 480
tcattaggtg acaaaatttc atctacaatc gttgcacaga gcgctcaagt ccccacaatg 540
ggttggagtg gtgatggtat taccgagaca gagtttgatg ctgcaggcca cgttatcgtc 600
cctgacaatg cttataacga agcctgtgtc aagactgccg agcaaggtct caaggctgcc 660
gagaagattg gtttccctgt catgatcaag gcctccgaag gtggtggagg taaaggtatt 720
cgtatggtca aggatggatc caactttgcc caattgtttg cccaagtgca aggtgaaatc 780
cctggttctc ccatctttat catgaagctg gctggtaacg ctcgccactt ggaagtccaa 840
ttgttggccg atcaatacgg taatgctatc agtttgtttg gtcgtgattg ttcagttcaa 900
cgtcgtcacc aaaagatcat cgaagaagct cctgtcacca ttgccaagcc tgatgtcttt 960
gagcaaatgg agaaggctgc tgtcagactc ggtaaattgg tgggctatgt ctctgctggt 1020
accgtggaat atctctacag tcatcacgac gatcaattct acttcttgga attgaaccct 1080
agattgcaag tcgagcatcc taccaccgag atggtttctg gcgtcaattt gcctgctgct 1140
cagctccaaa ttgctatggg tattcctctt caccgtatcc gcgatattcg tgtcctctac 1200
ggtgtccaac ccaacagtgc ctctgaaatc gatttcggtt tcgagcaccc cacctctctc 1260
accagccatc gtcgccccac ccccaagggc cacgttattg cttgtcgtat cactgctgag 1320
aatcccgatg ctggtttcaa gccttcttct ggtatcatgc aagaactcaa tttcagatcg 1380
agcacaaatg tttggggtta cttttctgtt gtctctgctg gtggtttgca cgagtatgct 1440
gattctcaat ttggtcacat ctttgcctat ggtgagaacc gtcaacaagc tcgcaagaac 1500
atggttattg ctctcaagga gctctccatc cgtgctgatt tccgtagtac cgttgaatac 1560
attatccgtt tgctcgagac ccctgatttc gaagagaaca ccatcaacac cggttggttg 1620
gatatgttga tttccaagaa gttgactgcc gaacgtcctg ataccatgtt ggctgtcttc 1680
tgtggtgctg tcaccaaggc tcatatggcc tctttggatt gtttccaaca atacaagcaa 1740
tccctcgaga agggtcaagt gcccagcaag ggttctctca agactgtctt tactgttgat 1800
ttcatctacg aggaagtgcg ctacaacttt actgtcaccc aatctgctcc tggtatctac 1860
accttgtatt tgaacggcac caagactcaa gttggtatcc gcgatttgtc tgatggcggt 1920
ctcttgatct ctattgacgg taaatctcac actacttact ctcgcgatga agttcaagcc 1980
acccgtatga tggtggatgg taagacctgt cttttggaaa aggagagtga tcctactcaa 2040
ttgagaagtc cttctcctgg taagcttgtc aacttgctcg ttgagaatgg cgatcatttg 2100
aatgctggtg atgcatatgc tgaaattgag gtcatgaaaa tgtacatgcc cttgattgct 2160
actgaagatg gtcatgttca attcatcaag caagctggtg ctactcttga ggctggtgat 2220
atcattggta tcttgtctct ggatgatcct agccgtgtca agcacgccct tcccttcaac 2280
ggcactgtac ctgcctttgg tgctcctcac atcactggtg acaagcctgt gcaacgcttc 2340
aacgctacca agctcacctt gcaacacatc ctccaaggtt acgataacca agctcttgtc 2400
caaactgttg tcaaggattt tgccgatatc ttgaacaacc ctgacttgcc ttactctgaa 2460
ttgaactctg ttctctctgc tttgtctggc agaattcctc aacgcttgga agcctctatc 2520
cacaagttgg ctgatgaatc caaggctgct aaccaagagt tccctgctgc tcaatttgaa 2580
aagcttgtcg aagactttgc tcgtgaacac atcacccttc aatctgaagc cactgcttac 2640
aagaactctg tcgctccctt gagcagcatc tttgctcgct atagaaacgg tctcaccgaa 2700
cacgcctaca gcaactatgt tgaattgatg gaagcttact acgacgtcga aatcttgttc 2760
aaccaacaac gcgaagagga ggtcatcttg tcactcagag atcagcacaa ggacgacttg 2820
gacaaggtgt tggctgttac tttgtctcat gccaaggtca acatcaagaa caatgtgatc 2880
ctcatgttgc tcgacctcat caaccctgtc tccactggca gtgctcttga caagtacttt 2940
actcctatcc ttaagcgttt gtctgaaatc gaaagccgtg ccactcaaaa ggtcactctc 3000
aaggcccgtg aattgttaat cctctgtcaa ttgccttctt acgaggaacg tcaagctcaa 3060
atgtaccaaa ttctcaagaa ctctgtcacc gagtctgtct acggcggtgg ttctgaatac 3120
cgcactccca gctacgacgc cttcaaggat ctcattgaca ccaagttcaa cgtctttgat 3180
gtcttgcctc acttcttcta ccatgctgat ccttatattg ccttggctgc tatcgaagta 3240
tactgtcgtc gttcttacca cgcttacaag attttggatg tcgcttacaa cctcgagcac 3300
aagccttacg ttgttgcctg gaagttcttg ctccaaactg ctgctaacgg cattgactcc 3360
aacaagcgta ttgcctctta ctctgatctt accttcttgc tcaacaagac tgaggaagag 3420
cctatccgta ctggtgccat gactgcatgc aactctttgg ctgatctcca agccgagctt 3480
cctcgcatct tgactgcctt tgaggaagag cctctccctc ccatgttgca acgcaatgct 3540
gctcccaagg aagaacgcat ggagaacatt ctcaacattg ctgttcgtgc tgatgaggac 3600
atggatgaca ctgctttcag aaccaagatc tgtgagatga tcactgccaa tgctgatgct 3660
ttccgtcaag cccatttgcg tcgtctttcc gttgtcgtct gccgtgataa ccaatggccc 3720
gattactaca ctttccgtga gcgtgaaaac taccaagaag acgagaccat ccgtcacatt 3780
gaacctgcca tggcctatca actcgagttg gctcgtctct ccaactttga catcaagccc 3840
tgtttcattg agaacagaca aatgcacgtc tactatgctg ttgccaagga gaacccctct 3900
gactgccgtt tcttcatccg tgccttggtc cgtcccggtc gtgtcaagag ctccatgcgc 3960
actgctgact acttgatctc tgagagtgat cgtctcttga ctgacattct tgacaccctc 4020
gaaattgtca gccacgagta caagaactct gactgtaacc acttgttcat caacttcatc 4080
cccacttttg ctattgaggc tgatgatgtg gagcacgcct tgaaggactt tgtcgatcgt 4140
catggtaagc gtctctggaa gctccgtgtc actggtgccg aaatccgctt caacgtgcaa 4200
tccaagaagc ctgatgcccc catcatcccc atgcgtttca ccgtggacaa cgtctctggc 4260
ttcatcttga aggtcgaggt ctaccaagaa gtcaagactg aaaagagtgg atggattctc 4320
aagtctgtca acaagatccc tggtgccatg cacatgcagc ccttgtctac gccttatcct 4380
accaaggaat ggctccaacc ccgtcgttac aaggctcact tgatgggcac cacctacgtc 4440
tatgacttcc ctgagctctt ccgtcaatct gttcaaaacc aatggactca agccatcaag 4500
cgcaaccctc tcttgaagca acccagccat ttggttgaag ccaaggagtt ggtcctcgac 4560
gaagatgatg tcttgcaaga gattgaccgt gctcccggta ccaacactgt cggtatggtt 4620
gcctggatca tgactattcg cactcccgag tatcccagtg gtcgtcgcat cattgctatt 4680
gccaacgaca ttaccttcaa gattggttcc tttggtgttg ctgaagatca agtcttttac 4740
aaggcatctg agctcgctcg tgctcttggt attcctcgta tctacttgtc tgccaactcg 4800
ggtgctcgta tcggtcttgc tgaagagctc atcagccaat tcagagctgc ctggaaggat 4860
gcttccaacc ctactgctgg tttcaactac ctctacttga ctcctgccga gtatgatact 4920
cttgttcaac aaggtgatgc caagagtgtg ctcgtggaag agatccaaga tgaaggtgaa 4980
acgcgtctcc gtatcactga tgtcattggc cacactgatg gtcttggtgt ggagaacttg 5040
aagggttctg gtctcattgc tggtgccacc tcgcgtgcct atgatgatat cttcaccatc 5100
acgcttgtca cttgtcgttc cgtcggtatt ggtgcttacc ttgtccgtct cggtcaaaga 5160
actgtccaaa acgaaggcca acccatcatc ttgaccggtg ctcctgcctt gaacaaggtg 5220
cttggtcgtg aagtctacac ctccaacttg cagttgggtg gtactcaaat catgtacaag 5280
aacggtgtct ctcacttgac tgccgagaat gatttggaag gtattgccaa gattgtgcaa 5340
tggctctcat tcgtgcctga tgtccgcaac gctcctgtct ctatgcgctt gggtgttgat 5400
cccattgacc gtgacattga gtacactcct cccaagggtc cctctgatcc tcgcttcttc 5460
ttggccggta agagtgagaa cggcaagtgg ttgagcggtt tctttgatca agactccttc 5520
attgagacct tgagtggttg ggctcgtacc gttgtcgttg gtcgtgctcg tttgggtggt 5580
attcctatgg gtgttgtctc tgttgagacc cgtactgtgg agaacattgt tcctgctgat 5640
cccgccaatt ctgattccac tgagcaagtc tttatggaag ctggtggtgt ctggttccct 5700
aactctgctt acaagactgc ccaagccatc aatgacttca acaagggtga gcaattgcct 5760
ttgatgatct ttgccaactg gcgtggtttc tctggtggtc aacgcgacat gtacaacgaa 5820
gtcctcaagt acggtgctca aatcgtggat gctctcagca actacaagca acctgtcttt 5880
gtctacatta ttcccaacgg tgagcttcgt ggtggtgctt gggtcgttgt tgatcctacc 5940
atcaacaagg acatgatgga aatgtacgct gataacaatg ctcgtggtgg tgtcttggag 6000
cccgagggta tcgtcgagat caagtaccgt aagcctgcct tgttggctac tatggagcgt 6060
cttgacgcta cttatgcttc tttgaagaag caattggccg aagaaggtaa atctgacgaa 6120
gagaaggctg ccctcaaggt gcaaatcgaa gctcgtgaac aagaactctt gcccgtatac 6180
caacaaatct ctatccaatt tgctgatctc catgatcgtg ctggtcgtat gaaggctaag 6240
ggtgtcatcc gcaaggctct tgactggcgt cgtgctcgtc actacttcta ctggcgtgtc 6300
cgtcgtcgtc tctgtgaaga gtacactttc cgcaagattg ttgctgccac tagcgctgct 6360
cccatgtctc gcgagcaaat gcttgatctc gtcaagcaat ggtttaccag cgacaatgaa 6420
actgccagct tcgaagatgc tgatgaactc gtctctgaat ggtttgaaaa gcgtgccagt 6480
gtcattgatc aacgtatcag caagttgaag agcgatgcta ccaaggaaca agtcgtgtct 6540
ctcggtaatg ccgatcaaga agctgtcatt gaaggtttca ctcaattgat tgaaaacttg 6600
agcgaagatc aacgtgctga aatcttgcgc aagcttaatt ctcgtttcta a 6651
<210> 33
<211> 6702
<212> DNA
<213> ACC1sc
<400> 33
atgagcgaag aaagcttatt cgagtcttct ccacagaaga tggagtacga aattacaaac 60
tactcagaaa gacatacaga acttccaggt catttcattg gcctcaatac agtagataaa 120
ctagaggagt ccccgttaag ggactttgtt aagagtcacg gtggtcacac ggtcatatcc 180
aagatcctga tagcaaataa tggtattgcc gccgtgaaag aaattagatc cgtcagaaaa 240
tgggcatacg agacgttcgg cgatgacaga accgtccaat tcgtcgccat ggccacccca 300
gaagatctgg aggccaacgc agaatatatc cgtatggccg atcaatacat tgaagtgcca 360
ggtggtacta ataataacaa ctacgctaac gtagacttga tcgtagacat cgccgaaaga 420
gcagacgtag acgccgtatg ggctggctgg ggtcacgcct ccgagaatcc actattgcct 480
gaaaaattgt cccagtctaa gaggaaagtc atctttattg ggcctccagg taacgccatg 540
aggtctttag gtgataaaat ctcctctacc attgtcgctc aaagtgctaa agtcccatgt 600
attccatggt ctggtaccgg tgttgacacc gttcacgtgg acgagaaaac cggtctggtc 660
tctgtcgacg atgacatcta tcaaaagggt tgttgtacct ctcctgaaga tggtttacaa 720
aaggccaagc gtattggttt tcctgtcatg attaaggcat ccgaaggtgg tggtggtaaa 780
ggtatcagac aagttgaacg tgaagaagat ttcatcgctt tataccacca ggcagccaac 840
gaaattccag gctcccccat tttcatcatg aagttggccg gtagagcgcg tcacttggaa 900
gttcaactgc tagcagatca gtacggtaca aatatttcct tgttcggtag agactgttcc 960
gttcagagac gtcatcaaaa aattatcgaa gaagcaccag ttacaattgc caaggctgaa 1020
acatttcacg agatggaaaa ggctgccgtc agactgggga aactagtcgg ttatgtctct 1080
gccggtaccg tggagtatct atattctcat gatgatggaa aattctactt tttagaattg 1140
aacccaagat tacaagtcga gcatccaaca acggaaatgg tctccggtgt taacttacct 1200
gcagctcaat tacaaatcgc tatgggtatc cctatgcata gaataagtga cattagaact 1260
ttatatggta tgaatcctca ttctgcctca gaaatcgatt tcgaattcaa aactcaagat 1320
gccaccaaga aacaaagaag acctattcca aagggtcatt gtaccgcttg tcgtatcaca 1380
tcagaagatc caaacgatgg attcaagcca tcgggtggta ctttgcatga actaaacttc 1440
cgttcttcct ctaatgtttg gggttacttc tccgtgggta acaatggtaa tattcactcc 1500
ttttcggact ctcagttcgg ccatattttt gcttttggtg aaaatagaca agcttccagg 1560
aaacacatgg ttgttgccct gaaggaattg tccattaggg gtgatttcag aactactgtg 1620
gaatacttga tcaaactttt ggaaactgaa gatttcgagg ataacactat taccaccggt 1680
tggttggacg atttgattac tcataaaatg accgctgaaa agcctgatcc aactcttgcc 1740
gtcatttgcg gtgccgctac aaaggctttc ttagcatctg aagaagcccg ccacaagtat 1800
atcgaatcct tacaaaaggg acaagttcta tctaaagacc tactgcaaac tatgttccct 1860
gtagatttta tccatgaggg taaaagatac aagttcaccg tagctaaatc cggtaatgac 1920
cgttacacat tatttatcaa tggttctaaa tgtgatatca tactgcgtca actatctgat 1980
ggtggtcttt tgattgccat aggcggtaaa tcgcatacca tctattggaa agaagaagtt 2040
gctgctacaa gattatccgt tgactctatg actactttgt tggaagttga aaacgatcca 2100
acccagttgc gtactccatc ccctggtaaa ttggttaaat tcttggtgga aaatggtgaa 2160
cacattatca agggccaacc atatgcagaa attgaagtta tgaaaatgca aatgcctttg 2220
gtttctcaag aaaatggtat cgtccagtta ttaaagcaac ctggttctac cattgttgca 2280
ggtgatatca tggctattat gactcttgac gatccatcca aggtcaagca cgctctacca 2340
tttgaaggta tgctgccaga ttttggttct ccagttatcg aaggaaccaa acctgcctat 2400
aaattcaagt cattagtgtc tactttggaa aacattttga agggttatga caaccaagtt 2460
attatgaacg cttccttgca acaattgata gaggttttga gaaatccaaa actgccttac 2520
tcagaatgga aactacacat ctctgcttta cattcaagat tgcctgctaa gctagatgaa 2580
caaatggaag agttagttgc acgttctttg agacgtggtg ctgttttccc agctagacaa 2640
ttaagtaaat tgattgatat ggccgtgaag aatcctgaat acaaccccga caaattgctg 2700
ggcgccgtcg tggaaccatt ggcggatatt gctcataagt actctaacgg gttagaagcc 2760
catgaacatt ctatatttgt ccatttcttg gaagaatatt acgaagttga aaagttattc 2820
aatggtccaa atgttcgtga ggaaaatatc attctgaaat tgcgtgatga aaaccctaaa 2880
gatctagata aagttgcgct aactgttttg tctcattcga aagtttcagc gaagaataac 2940
ctgatcctag ctatcttgaa acattatcaa ccattgtgca agttatcttc taaagtttct 3000
gccattttct ctactcctct acaacatatt gttgaactag aatctaaggc taccgctaag 3060
gtcgctctac aagcaagaga aattttgatt caaggcgctt taccttcggt caaggaaaga 3120
actgaacaaa ttgaacatat cttaaaatcc tctgttgtga aggttgccta tggctcatcc 3180
aatccaaagc gctctgaacc agatttgaat atcttgaagg acttgatcga ttctaattac 3240
gttgtgttcg atgttttact tcaattccta acccatcaag acccagttgt gactgctgca 3300
gctgctcaag tctatattcg tcgtgcttat cgtgcttaca ccataggaga tattagagtt 3360
cacgaaggtg tcacagttcc aattgttgaa tggaaattcc aactaccttc agctgcgttc 3420
tccacctttc caactgttaa atctaaaatg ggtatgaaca gggctgtttc tgtttcagat 3480
ttgtcatatg ttgcaaacag tcagtcatct ccgttaagag aaggtatttt gatggctgtg 3540
gatcatttag atgatgttga tgaaattttg tcacaaagtt tggaagttat tcctcgtcac 3600
caatcttctt ctaacggacc tgctcctgat cgttctggta gctccgcatc gttgagtaat 3660
gttgctaatg tttgtgttgc ttctacagaa ggtttcgaat ctgaagagga aattttggta 3720
aggttgagag aaattttgga tttgaataag caggaattaa tcaatgcttc tatccgtcgt 3780
atcacattta tgttcggttt taaagatggg tcttatccaa agtattatac ttttaacggt 3840
ccaaattata acgaaaatga aacaattcgt cacattgagc cggctttggc cttccaactg 3900
gaattaggaa gattgtccaa cttcaacatt aaaccaattt tcactgataa tagaaacatc 3960
catgtctacg aagctgttag taagacttct ccattggata agagattctt tacaagaggt 4020
attattagaa cgggtcatat ccgtgatgac atttctattc aagaatatct gacttctgaa 4080
gctaacagat tgatgagtga tatattggat aatttagaag tcaccgacac ttcaaattct 4140
gatttgaatc atatcttcat caacttcatt gcggtgtttg atatctctcc agaagatgtc 4200
gaagccgcct tcggtggttt cttagaaaga tttggtaaga gattgttgag attgcgtgtt 4260
tcttctgccg aaattagaat catcatcaaa gatcctcaaa caggtgcccc agtaccattg 4320
cgtgccttga tcaataacgt ttctggttat gttatcaaaa cagaaatgta caccgaagtc 4380
aagaacgcaa aaggtgaatg ggtatttaag tctttgggta aacctggatc catgcattta 4440
agacctattg ctactcctta ccctgttaag gaatggttgc aaccaaaacg ttataaggca 4500
cacttgatgg gtaccacata tgtctatgac ttcccagaat tattccgcca agcatcgtca 4560
tcccaatgga aaaatttctc tgcagatgtt aagttaacag atgatttctt tatttccaac 4620
gagttgattg aagatgaaaa cggcgaatta actgaggtgg aaagagaacc tggtgccaac 4680
gctattggta tggttgcctt taagattact gtaaagactc ctgaatatcc aagaggccgt 4740
caatttgttg ttgttgctaa cgatatcaca ttcaagatcg gttcctttgg tccacaagaa 4800
gacgaattct tcaataaggt tactgaatat gctagaaagc gtggtatccc aagaatttac 4860
ttggctgcaa actcaggtgc cagaattggt atggctgaag agattgttcc actatttcaa 4920
gttgcatgga atgatgctgc caatccggac aagggcttcc aatacttata cttaacaagt 4980
gaaggtatgg aaactttaaa gaaatttgac aaagaaaatt ctgttctcac tgaacgtact 5040
gttataaacg gtgaagaaag atttgtcatc aagacaatta ttggttctga agatgggtta 5100
ggtgtcgaat gtctacgtgg atctggttta attgctggtg caacgtcaag ggcttaccac 5160
gatatcttca ctatcacctt agtcacttgt agatccgtcg gtatcggtgc ttatttggtt 5220
cgtttgggtc aaagagctat tcaggtcgaa ggccagccaa ttattttaac tggtgctcct 5280
gcaatcaaca aaatgctggg tagagaagtt tatacttcta acttacaatt gggtggtact 5340
caaatcatgt ataacaacgg tgtttcacat ttgactgctg ttgacgattt agctggtgta 5400
gagaagattg ttgaatggat gtcttatgtt ccagccaagc gtaatatgcc agttcctatc 5460
ttggaaacta aagacacatg ggatagacca gttgatttca ctccaactaa tgatgaaact 5520
tacgatgtaa gatggatgat tgaaggtcgt gagactgaaa gtggatttga atatggtttg 5580
tttgataaag ggtctttctt tgaaactttg tcaggatggg ccaaaggtgt tgtcgttggt 5640
agagcccgtc ttggtggtat tccactgggt gttattggtg ttgaaacaag aactgtcgag 5700
aacttgattc ctgctgatcc agctaatcca aatagtgctg aaacattaat tcaagaacct 5760
ggtcaagttt ggcatccaaa ctccgccttc aagactgctc aagctatcaa tgactttaac 5820
aacggtgaac aattgccaat gatgattttg gccaactgga gaggtttctc tggtggtcaa 5880
cgtgatatgt tcaacgaagt cttgaagtat ggttcgttta ttgttgacgc attggtggat 5940
tacaaacaac caattattat ctatatccca cctaccggtg aactaagagg tggttcatgg 6000
gttgttgtcg atccaactat caacgctgac caaatggaaa tgtatgccga cgtcaacgct 6060
agagctggtg ttttggaacc acaaggtatg gttggtatca agttccgtag agaaaaattg 6120
ctggacacca tgaacagatt ggatgacaag tacagagaat tgagatctca attatccaac 6180
aagagtttgg ctccagaagt acatcagcaa atatccaagc aattagctga tcgtgagaga 6240
gaactattgc caatttacgg acaaatcagt cttcaatttg ctgatttgca cgataggtct 6300
tcacgtatgg tggccaaggg tgttatttct aaggaactgg aatggaccga ggcacgtcgt 6360
ttcttcttct ggagattgag aagaagattg aacgaagaat atttgattaa aaggttgagc 6420
catcaggtag gcgaagcatc aagattagaa aagatcgcaa gaattagatc gtggtaccct 6480
gcttcagtgg accatgaaga tgataggcaa gtcgcaacat ggattgaaga aaactacaaa 6540
actttggacg ataaactaaa gggtttgaaa ttagagtcat tcgctcaaga cttagctaaa 6600
aagatcagaa gcgaccatga caatgctatt gatggattat ctgaagttat caagatgtta 6660
tctaccgatg ataaagaaaa attgttgaag actttgaaat aa 6702
<210> 34
<211> 6801
<212> DNA
<213> ACCyl
<400> 34
atgcgactgc aattgaggac actaacacgt cggtttttca gtatggcttc aggatcttca 60
acgccagatg tggctccctt ggtggacccc aacattcaca aaggtctcgc ctctcatttc 120
tttggactca attctgtcca cacagccaag ccctcaaaag tcaaggagtt tgtggcttct 180
cacggaggtc atacagttat caacaaggtc ctcatcgcta acaacggtat tgccgcagta 240
aaggagatcc gttcagtacg aaaatgggcc tacgagacct ttggcgacga gcgagcaatc 300
tcgttcaccg tcatggccac ccccgaagat ctcgctgcca acgccgacta cattagaatg 360
gccgatcagt acgtcgaggt gcccggagga accaacaaca acaactacgc caacgtcgag 420
ctgattgtcg acgtggctga gcgattcggc gtcgatgccg tgtgggccgg atggggccat 480
gccagtgaaa atcccctgct ccccgagtcg ctagcggcct ctccccgcaa gattgtcttc 540
atcggccctc ccggagctgc catgagatct ctgggagaca aaatttcttc taccattgtg 600
gcccagcacg caaaggtccc gtgtatcccg tggtctggaa ccggagtgga cgaggttgtg 660
gttgacaaga gcaccaacct cgtgtccgtg tccgaggagg tgtacaccaa gggctgcacc 720
accggtccca agcagggtct ggagaaggct aagcagattg gattccccgt gatgatcaag 780
gcttccgagg gaggaggagg aaagggtatt cgaaaggttg agcgagagga ggacttcgag 840
gctgcttacc accaggtcga gggagagatc cccggctcgc ccatcttcat tatgcagctt 900
gcaggcaatg cccggcattt ggaggtgcag cttctggctg atcagtacgg caacaatatt 960
tcactgtttg gtcgagattg ttcggttcag cgacggcatc aaaagattat tgaggaggct 1020
cctgtgactg tggctggcca gcagaccttc actgccatgg agaaggctgc cgtgcgactc 1080
ggtaagcttg tcggatatgt ctctgcaggt accgttgaat atctgtattc ccatgaggac 1140
gacaagttct acttcttgga gctgaatcct cgtcttcagg tcgaacatcc taccaccgag 1200
atggtcaccg gtgtcaacct gcccgctgcc cagcttcaga tcgccatggg tatccccctc 1260
gatcgaatca aggacattcg tctcttttac ggtgttaacc ctcacaccac cactccaatt 1320
gatttcgact tctcgggcga ggatgctgat aagacacagc gacgtcccgt cccccgaggt 1380
cacaccactg cttgccgaat cacatccgag gaccctggag agggtttcaa gccctccgga 1440
ggtactatgc acgagctcaa cttccgatcc tcgtccaacg tgtggggtta cttctccgtt 1500
ggtaaccagg gaggtatcca ttcgttctcg gattcgcagt ttggtcacat cttcgccttc 1560
ggtgagaacc gaagtgcgtc tcgaaagcac atggttgttg ctttgaagga actatctatt 1620
cgaggtgact tccgaaccac cgtcgagtac ctcatcaagc tgctggagac accggacttc 1680
gaggacaaca ccatcaccac cggctggctg gatgagctta tctccaacaa gctgactgcc 1740
gagcgacccg actcgttcct cgctgttgtt tgtggtgctg ctaccaaggc ccatcgagct 1800
tccgaggact ctattgccac ttacatggct tcgctagaga agggccaggt ccctgctcga 1860
gacattctca agaccctttt ccccgttgac ttcatctacg agggccagcg gtacaagttc 1920
accgccaccc ggtcgtctga ggactcttac acgctgttca tcaacggttc tcgatgcgac 1980
attggagtta gacctctttc tgacggtggt attctgtgtc ttgtaggtgg gagatcccac 2040
aatgtctact ggaaggagga ggttggagcc acgcgactgt ctgttgactc caagacctgc 2100
cttctcgagg tggagaacga tcccactcag cttcgatctc cctctcccgg taagctggtt 2160
aagttcctgg tcgagaacgg cgaccacgtg cgagccaacc agccctatgc cgagattgag 2220
gtcatgaaga tgtacatgac tctcactgct caggaggacg gtattgtcca gctgatgaag 2280
cagcccggtt ccaccatcga ggctggcgac atcctcggta tcttggccct tgatgatcct 2340
tccaaggtca agcatgccaa gccctttgag ggccagcttc ccgagcttgg accccccact 2400
ctcagcggta acaagcctca tcagcgatac gagcactgcc agaacgtgct ccataacatt 2460
ctgcttggtt tcgataacca ggtggtgatg aagtccactc ttcaggagat ggttggtctg 2520
ctccgaaacc ctgagcttcc ttatctccag tgggctcatc aggtgtcttc tctgcacacc 2580
cgaatgagcg ccaagctgga tgctactctt gctggtctca ttgacaaggc caagcagcga 2640
ggtggcgagt ttcctgccaa gcagcttctg cgagcccttg agaaggaggc gagctctggc 2700
gaggtcgatg cgctcttcca gcaaactctt gctcctctgt ttgaccttgc tcgagagtac 2760
caggacggtc ttgctatcca cgagcttcag gttgctgcag gccttctgca ggcctactac 2820
gactctgagg cccggttctg cggacccaac gtacgtgacg aggatgtcat tctcaagctt 2880
cgagaggaga accgagattc tcttcgaaag gttgtgatgg cccagctgtc tcattctcga 2940
gtcggagcca agaacaacct tgtgctggcc cttctcgatg aatacaaggt ggccgaccag 3000
gctggcactg actctcctgc ctccaacgtg cacgttgcaa agtacttgcg acctgtgctg 3060
cgaaagattg tggagctgga atctcgagct tctgccaagg tatctctgaa agcccgagag 3120
atcctcatcc agtgcgctct gccctctcta aaggagcgaa ctgaccagct tgagcacatt 3180
ctgcgatctt ctgtcgtcga gtctcgatac ggagaggttg gtctggagca ccgaactccc 3240
cgagccgata ttctcaagga ggttgtcgac tccaagtaca ttgtctttga tgtgcttgcc 3300
cagttctttg cccacgatga tccctggatc gtccttgctg ccctggagct gtacatccga 3360
cgagcttgca aggcctactc catcctggac atcaactacc accaggactc ggacctgcct 3420
cccgtcatct cgtggcgatt tagactgcct accatgtcgt ctgctttgta caactcagta 3480
gtgtcttctg gctccaaaac ccccacttcc ccctcggtgt ctcgagctga ttccgtctcc 3540
gacttttcgt acaccgttga gcgagactct gctcccgctc gaaccggagc gattgttgcc 3600
gtgcctcatc tggatgatct ggaggatgct ctgactcgtg ttctggagaa cctgcccaaa 3660
cggggcgctg gtcttgccat ctctgttggt gctagcaaca agagtgccgc tgcttctgct 3720
cgtgacgctg ctgctgctgc cgcttcatcc gttgacactg gcctgtccaa catttgcaac 3780
gttatgattg gtcgggttga tgagtctgat gacgacgaca ctctgattgc ccgaatctcc 3840
caggtcattg aggactttaa ggaggacttt gaggcctgtt ctctgcgacg aatcaccttc 3900
tccttcggca actcccgagg tacttatccc aagtatttca cgttccgagg ccccgcatac 3960
gaggaggacc ccactatccg acacattgag cctgctctgg ccttccagct ggagctcgcc 4020
cgtctgtcca acttcgacat caagcctgtc cacaccgaca accgaaacat ccacgtgtac 4080
gaggctactg gcaagaacgc tgcttccgac aagcggttct tcacccgagg tatcgtacga 4140
cctggtcgtc ttcgagagaa catccccacc tcggagtatc tcatttccga ggctgaccgg 4200
ctcatgagcg atattttgga cgctctagag gtgattggaa ccaccaactc ggatctcaac 4260
cacattttca tcaacttctc agccgtcttt gctctgaagc ccgaggaggt tgaagctgcc 4320
tttggcggtt tcctggagcg atttggccga cgtctgtggc gacttcgagt caccggtgcc 4380
gagatccgaa tgatggtatc cgaccccgaa actggctctg ctttccctct gcgagcaatg 4440
atcaacaacg tctctggtta cgttgtgcag tctgagctgt acgctgaggc caagaacgac 4500
aagggccagt ggattttcaa gtctctgggc aagcccggct ccatgcacat gcggtctatc 4560
aacactccct accccaccaa ggagtggctg cagcccaagc ggtacaaggc ccatctgatg 4620
ggtaccacct actgctatga cttccccgag ctgttccgac agtccattga gtcggactgg 4680
aagaagtatg acggcaaggc tcccgacgat ctcatgactt gcaacgagct gattctcgat 4740
gaggactctg gcgagctgca ggaggtgaac cgagagcccg gcgccaacaa cgtcggtatg 4800
gttgcgtgga agtttgaggc caagaccccc gagtaccctc gaggccgatc tttcatcgtg 4860
gtggccaacg atatcacctt ccagattggt tcgtttggcc ctgctgagga ccagttcttc 4920
ttcaaggtga cggagctggc tcgaaagctc ggtattcctc gaatctatct gtctgccaac 4980
tctggtgctc gaatcggcat tgctgacgag ctcgttggca agtacaaggt tgcgtggaac 5040
gacgagactg acccctccaa gggcttcaag tacctttact tcacccctga gtctcttgcc 5100
accctcaagc ccgacactgt tgtcaccact gagattgagg aggagggtcc caacggcgtg 5160
gagaagcgtc atgtgatcga ctacattgtc ggagagaagg acggtctcgg agtcgagtgt 5220
ctgcggggct ctggtctcat tgcaggcgcc acttctcgag cctacaagga tatcttcact 5280
ctcactcttg tcacctgtcg atccgttggt atcggtgctt accttgttcg tcttggtcaa 5340
cgagccatcc agattgaggg ccagcccatc attctcactg gtgcccccgc catcaacaag 5400
ctgcttggtc gagaggtcta ctcttccaac ttgcagcttg gtggtactca gatcatgtac 5460
aacaacggtg tgtctcatct gactgcccga gatgatctca acggtgtcca caagatcatg 5520
cagtggctgt catacatccc tgcttctcga ggtcttccag tgcctgttct ccctcacaag 5580
accgatgtgt gggatcgaga cgtgacgttc cagcctgtcc gaggcgagca gtacgatgtt 5640
agatggctca tttctggccg aactctcgag gatggtgctt tcgagtctgg tctctttgac 5700
aaggactctt tccaggagac tctgtctggc tgggccaagg gtgttgttgt tggtcgagct 5760
cgtcttggcg gcattccctt cggtgtcatt ggtgtcgaga ctgcgaccgt cgacaatact 5820
acccctgccg atcccgccaa cccggactct attgagatga gcacctctga agccggccag 5880
gtttggtacc ccaactcggc cttcaagacc tctcaggcca tcaacgactt caaccatggt 5940
gaggcgcttc ctctcatgat tcttgctaac tggcgaggct tttctggtgg tcagcgagac 6000
atgtacaatg aggttctcaa gtacggatct ttcattgttg atgctctggt tgactacaag 6060
cagcccatca tggtgtacat ccctcccacc ggtgagctgc gaggtggttc ttgggttgtg 6120
gttgacccca ccatcaactc ggacatgatg gagatgtacg ctgacgtcga gtctcgaggt 6180
ggtgtgctgg agcccgaggg aatggtcggt atcaagtacc gacgagacaa gctactggac 6240
accatggctc gtctggatcc cgagtactcc tctctcaaga agcagcttga ggagtctccc 6300
gattctgagg agctcaaggt caagctcagc gtgcgagaga agtctctcat gcccatctac 6360
cagcagatct ccgtgcagtt tgccgacttg catgaccgag ctggccgaat ggaggccaag 6420
ggtgtcattc gtgaggctct tgtgtggaag gatgctcgtc gattcttctt ctggcgaatc 6480
cgacgacgat tagtcgagga gtacctcatt accaagatca atagcattct gccctcttgc 6540
actcggcttg agtgtctggc tcgaatcaag tcgtggaagc ctgccactct tgatcagggc 6600
tctgaccggg gtgttgccga gtggtttgac gagaactctg atgccgtctc tgctcgactc 6660
agcgagctca agaaggacgc ttctgcccag tcgtttgctt ctcaactgag aaaggaccga 6720
cagggtactc tccagggcat gaagcaggct ctcgcttctc tttctgaggc tgagcgggct 6780
gagctgctca aggggttgtg a 6801
<210> 35
<211> 6732
<212> DNA
<213> ACC1ch
<400> 35
atgagcgaag aaaacctgtt cgaggacgcc gccgcaaagc gccagtacga gataacagac 60
tactccacca agcacaagac cctcgcgtcc cacttcgtcg gcctcaacac cctcgagcgc 120
gtcgaggagt cccccctcaa ggacttcgtc aagtcccacg gcggtcacac cgtcatctcc 180
aagatcctca tcgccaacaa cggtatcgcc gccgtcaagg agatccgctc cgtcagaaag 240
tgggcctacg agaccttcgg cgatgagcgc accgtccagt tcgtcgccat ggccaccccc 300
gaggacctcc aggccaactc cgagtacatc cgcatggcgg accagtacgt cgaggtcccc 360
ggcggcacca acaacaacaa ctacgccaac gtcgacctca tcgtcgacgt cgccgagaga 420
accgacgtcg acgcagtctg ggccggctgg ggccacgcct cagagaaccc acacctccca 480
gagaagctcg ccgcctcaaa gagaaagatc atcttcatcg gtccacccgg cagcgccatg 540
cgctccctcg gtgacaagat ctcctccacc atcgtcgctc agcatgccaa ggtcccatgt 600
ataccttggt ccggcaccgg cgtcgacaca gtccacctcg acagcgagac cggcctagtg 660
tccgtcgacg acgacatcta ccagaagggc tgctgctcct cccccgagga cggcctcgcc 720
aaggccaaga agatcggctt cccagtaatg atcaaggcct ccgagggtgg tggtggtaag 780
ggtatcagac aggtcgagcg cgaggaggac ttcatcgccc tgtaccacca ggccgccaac 840
gagatcccgg gctccccaat attcatcatg aagctcgccg gcaaggcccg ccacctggag 900
gtccagcttc tcgccgatca gtacggtacc aacatttccc tgtttggccg tgactgttcc 960
gtgcagagac gccaccagaa gatcatcgag gaggcgccag tgactatcgc cagcaacgag 1020
acattcgaga agatggccag cgcagccgtc agactcggta agctggtcgg ctacgtctcc 1080
gccggtaccg tcgaatacct atattccagc gaggagaaga agtactactt cctggagctt 1140
aatccaagac tgcaggtcga gcacccaacc accgagatgg tctccggtgt caacctgcct 1200
tccgcccagc tgcagatcgc catgggtatc ccaatgcaca gaatcagcga catcagagtc 1260
ctttacggta tgaaccctca cggcgccacc gagatcgact tcgagttcaa gactgaggag 1320
gccaagaaga ctcagagaaa gcctgttcca aagggccatt gtaccgcttg tcgtatcact 1380
tccgaggatc caaacgaggg tttcaagcct tccggtggtt ccctacacga gctgaacttc 1440
agatcttctt caaatgtctg gggttacttc tccgtcggta acaacggtgg tatccactcc 1500
ttctccgact cccaattcgg tcacatcttc gccttcggtg agaacagaca gatgtcgaga 1560
aagcacatgg ttgtcgccct aaaggagctg tccatcagag gtgacttcag aactactgtc 1620
gagtatctga tcaagcttct agagactgag gatttcgagg agaacactat tactaccggt 1680
tggttggacg acctgatctc gcacaagatg accgcccaga agccagatgc tacgcttgcc 1740
gtcatttgtg gcgctgctac caaggctttc atcgcgtcag agtctgccag aaaggaatac 1800
attaaccagc tcaagagagg tcaggttcca ccaaagggcc tactggagac tatgttccca 1860
attgaattca tccacgagaa caagagatat aagttcactg ttgccaagtc tgctgacgat 1920
tcctacacac ttttcatcaa tggttccaag tgcgaggtcc gtgctgttaa gctatctgat 1980
ggcggtcttt tgatttctgt cggcggccaa tctcacacta tctactggaa ggaggaagcc 2040
caggctacca gactttcgat cgactctatg actactctac ttgaggttga gaatgatcct 2100
acacagctgc gcaccccatc cccaggtaag cttgtgaagt tccttgttga gaacggtgac 2160
caaatcaagg tcggccagcc atacgctgag attgaggtca tgaagatgca aatgccattg 2220
gtttctcagg agaacggtgt tatccagtta ctaaagcagc caggttctac cattgacgct 2280
ggtgatatca ttgccatgct tgcgctagac gacccatcca aggtcaagca cgctatgcca 2340
tttgagggta tgcttccaga gctaggctct ccaatggttg aaggtaccaa gccagcctac 2400
aagttccact ccctagtgtc cacgcttgag aatattctaa agggttacga caaccaggtt 2460
atcatgaacg catctctaca acagctaatc gaggttctca gagacccaag actaccatac 2520
tctgagtgga agttaaatgt atctgcgcta cacaccagac ttcctcttgc tttggacgag 2580
aagttggaca agctggtcga gcgttctgct aaccgcagtg ctatcttccc tgctaagcag 2640
ctacagaaga tgatcgatga tgctgtgaga gacccacaag ctgacccact attgtcatcc 2700
attgttgagc cactccttga cattactact cgttatgcca atggcctaga ggcacatgaa 2760
cattccgtct ttgtcaagtt cttagaggag tactacaacg tcgagaagct attcagcgag 2820
cacggtgagc gtgctgagaa tgtcatccta aagctacgtg acgagaacat cgatgaccta 2880
gacaaggttg ttctcattgt tctctctcat gccaaggttt ctgccaagaa cactctgatc 2940
ctagctattc tgaagcacta ccaaccaatt tgcaaaatgt cttcgtctgt tgcccatatc 3000
ttcaccaagc cacttcagca cattgtcgag ctggagtcca agcctgctgc taaggttgct 3060
ctacaggcca gagaaattct gatccagggt gccctaccat ctgtcaagga gagaacagag 3120
cagattgaac acattctaag atcttctgtc acaaacactt cttatggtga ctcttcgcca 3180
aaggtcagtg agccaaacat ggaaatactg aaggatctaa ttgactccaa ctatgtcgtt 3240
tttgatgttc tactcccatt cctgtgtcac gaagaccctg ttattgctac tgccgctggt 3300
caggtttatg ttcgccgtgc ttacagagct tacaccgttg gtgaggttaa gtcacacagc 3360
attactgact cttccatgaa cccattgatt gagtggaagt tccagctacc ctctgctgct 3420
ttctcttcca ttccacagct gaagagcaag attggtatga acagagctat gtctgtttct 3480
gacttgtcct acgttgttga cagtgagcac gctccactga gaactggtat attggttgct 3540
gctgaacatc tagatgaggt tgacgccagc cttgcacagg gcctagaggt tatcccagag 3600
cactccgctc caggcaatgg tccagctcca gacagatctg gcagttcctc ctctttgagt 3660
aacatcgtta atgtctgtgt tgccagcaca gaaggattcg attccgaaaa ggagattctt 3720
gccagagtga acgagatcct agagctgaac aaggctgatc taatcaagtc ttccatccgt 3780
cgtatcactt tcatgttcgg tttcccagac ggtacttatc caaagtatta cactttcaat 3840
ggtccaaact accaggagaa ccagacgatt cgtcacattg agccagcttg tgcgttccaa 3900
ctagaattag gaagaatgtc taacttcaac atcactccaa tcttcactga caacagaaac 3960
atccacgttt acgaagctgt cagtaagact tctgcactag acaagagatt cttcactaga 4020
ggtataatca gaaccggtcg cattcgtgac gagatttcca ttcaagaata tctgacctct 4080
gaagctaaca agctgatgag tgacattttg gacaacctgg agatcatcga cacttcgaac 4140
tccgacttga accacatctt tatcaacttt tctgctgtct ttgatgtgtc tccagaggat 4200
gtcgaggctg cctttggtgg cttcctagaa agattcggta agagattcct aagactacgt 4260
gtctctgctg ctgagattcg catcattatc caggacccaa agactggtgc accagtgcca 4320
ttgcgtgccc tgatcaacaa cgtctctggt tatgtcgtga agtctgagct atacaccgag 4380
atcaagaatg ccaagggtga gtgggtcttt aagtctctcg gtaagccagg ttctatgcat 4440
ctgagaccaa ttgctactcc atacccagtt aaggagtggc tacagccaaa gcgttacaag 4500
gctcacctaa tgggcacaac ttacgtttac gatttcccag agctcttcca ccaggctgct 4560
atcactacat ggcagaagta caagaccaac tcttctcaaa aggatctaac cacaatgaag 4620
gagagagatg atctgtttat ttcccacgag ctgattgagg atgagagcgg tgaactcact 4680
gaggttgaga gagaacctgg tgctaattca attggtatgg ttgccttcaa ggtcaccatg 4740
aagactcctg agtacccaag aggccgtcaa tttgtcattg tcgctaacga tatcacctat 4800
aagattggtt cctttggtcc acaagaggac gaattcttca acaaggttac cgagtacgcc 4860
agaaagagag gtattccaag aatctatcta gctgctaact ctggtgctag aatcggtatt 4920
gctgaggagc ttgttccact ataccaggtt gcctggaagg acgctgatgt tccagcgaag 4980
ggtttcgagt acatctacct gaacaaggat gaaatggaga ctctaaaggg tcactccaag 5040
gagaactctg tcatcaccga gcgcatcgtc gaggacggtg aggagagatt tgttatcaag 5100
gctattattg gtgctgacga cggcctaggt gttgagtgtt tgagaggttc tggtttgatt 5160
gctggtgcaa cttcgagagc ctaccaggac attttcacta ttactttggt tacctgcaga 5220
tccgttggta ttggtgctta tctagtcaga cttggccaga gagctattca gattgagggt 5280
cagcctatca tcctaactgg tgctcctgcc atcaacaagg ttctaggtag agaggtctac 5340
tcctccaacc tccaactcgg tggtacacag atcatgtaca acaacggtgt ttcccacttg 5400
actgcctctg acgatcttca ggcggttgag cagatcatta agtggttgtc ctacgtccct 5460
gcaaagcgca acatgccagt tccaatccta gagaacggcg ataagtggga cagacagatt 5520
gactacagac caactgttaa tgagcagtat gatgttcgct ggatgattga gggtcgcgaa 5580
ctagaagacg gtaggtttga atatggtatg ttcgacaagg gttctttctt tgagactctg 5640
tcgggctggg ctaagggtgt cgttgtcggc cgtgcccgtc taggcggtat tccaatcggt 5700
gttattggtg tggagaccag gaccatcgag aacgttatcc cagcggaccc tgccaaccca 5760
gactctactg aggctgttgt tcaggaagcc ggtcaagtgt ggtaccccaa ctctgctttc 5820
aagactgctc aggccatcaa ggactttaac catggtgagc agctgccgct aatgattcta 5880
gccaactgga gaggtttctc tggtggtcag cgcgacatgt acaacgaggt tctgaagtac 5940
ggttctttca ttgtcgactc cttagtggac tacaagcagc caatcttcat ctacattcca 6000
ccaaccggtg agttgcgtgg tggttcttgg gtcgttgttg accctactat caacgccgac 6060
cagatggaga tgtacgccga taaggactcc cgtgctggtg ttctagagcc aagtggtatg 6120
gttggtatta agtaccgtcg cgagaagcta ctggctacga tgaccagact cgacaccact 6180
taccgcgaac taaaggagca gctatccgac aagacgctaa cccctgctca acaccaggag 6240
gtatctgtga agcttgctga gcgtgagaag ttcctactac caatttacac tcaggtaagt 6300
gttcagtttg ctgatctgca tgaccgctct tctcgtatgg tgaagaaggg tgttatccgt 6360
gctgagctga cgtgggagga gtcccgtcgc ttcttcttct ggagactgag acgcaagctt 6420
gacgaggaat acctcgttaa gcgtctggac attgagttgc ctcacgcatc gcgtctggaa 6480
aagctggcgc gtctgcgttc atggtaccca gcctctatcg atgagaccaa ggaccgtgat 6540
gttgtgacat ggattgaaga gaactacaat gtgtttgagg aggagttcaa ggccttgaag 6600
cttgagtctt ttgcacagga gcttgccaag agcatgcgta ctgatcacga tgttacgatg 6660
cgcggtctcg aggagattgc ccgcatgttg tctaaggagg acaaggagag gctgctgagt 6720
gcgctaaagt aa 6732
<210> 36
<211> 3690
<212> DNA
<213> HPDH-MCRbs
<400> 36
atgaccgctg gtaaaggtaa aggtgctaaa gttgaccctg ctggtggtaa agccgctact 60
ggtagattgg ccggtaaagt tgcattggtc acaggtgcag ctggtaattt gggtggtgcc 120
attattagaa gattcattgc cgaaggtgca actttggtta tgactggtag aactaaggaa 180
agattagctg cagcaaagca agctttattg gacgaaactg gtgttgaaga agcaagagtt 240
tttaccgtaa tcttggatgg tgctgatcca gattcagtta gagcaggtat tgatcaagtc 300
agagctgcct taggtagaat tgatgttttg gtcaataatg caggttcagc tggtcctaga 360
caaacattgg aaaacgtacc attgactaga gccgaaatgg aagcaaatgg tgactcagaa 420
acagtagctg acgccatgag aaatttgttg ggtgtcagtt ggaacttaac aagagcagct 480
gccccaataa tgccagtagg tgcatcagtc gttaatattt ccaccatctt ctctcacaca 540
agatactatg gtagaacagc ctatgtcgtt cctaaggctg cattaaacgc cttatcccaa 600
gaattagcaa aggaattagg tgcatctggt ataagagtaa atacagtttt cccaggtcca 660
atcgaaagtg aaagaattag aacagttttc gcagctatgg atgccttgca aggtgaacca 720
gaaaactcta ctgcaaatca tttcatgggt agaatgacat tggaaagagc tatcggtgac 780
gcccctaaaa gaaagacttt gccagttcca ggtgacattg cagatgcttg tttgttttta 840
gcatcagatg aaagtgctgc cttaaatggt aacgaaatag atgttacaca tggtatgcaa 900
gttagaaaag atagtagatc aacttacatg actagaccat ccatgagatc tttggacggt 960
gcaggtttga ccgtcttaat tgccgctggt gaagcatggg aagatgcatt agatatggct 1020
aagatccaag ctgcatgtgg tgccagagtc ttattgggta tgactagaca agttgacgtc 1080
gcacaagccg aagccagagt taaagctcaa ggtgctgaag aaggtttaac catcatgaga 1140
ttcaacagag ctgaacctga tggtatggaa gcagccataa gagcctattc tgatgcacat 1200
ggtgcaatcc actctgccat cgttttacct gtaaagccag ctggtcattt cggtgactct 1260
ttggttgaag cttcagatgc agaagttgac gctttgattg atatcgaatt aaccggtgct 1320
attgcagttg ccagaacctt gagaagatac tggaaaacat gctctgactt gatgcaagat 1380
ccaagattcg tctttatgtc aggtccatct gatggtaaag gtaacaaagc aggtagaata 1440
ttagcagccg caatagccca attaatcaca atttggagag atgaatctag agtagacttt 1500
gcacaaggta gacaaaagag agccgcatgg ggtaatcaaa tagttagata cacaaatgaa 1560
gaagatgaaa acaccagatt cgccgcaggt catgctacta gaattgtttt caaggaacaa 1620
aagataactg aaacttcctt gtacttgcct gccaacattg gtgaagaaac tggtgctaga 1680
agagctatgt taggttttgc cgaaaatatc actggtttgc atttgggtaa agtcgccatg 1740
attacaggtg gttctgcagg tattggtggt caagttgcca gattattggc attggctggt 1800
gccaaagtta tgatggtagc tagaagagca agtgaattgg aagccgcaag agaaagaata 1860
gttggtgaat tgcaagatgt tggttttagt ggtgttgaaa gaagagttaa aatcatggca 1920
gatgttgacg tatccgactt tgattcttta agaagagctg ccgatgcaac tatggcagaa 1980
tttggtagaa tagactactt gattaataac gctggtgttg caggtgctga agatatggtt 2040
attgacatgt cattggatat gtggagatgg acattggacg ccaacttgat ttcaaactat 2100
ttgttaatgc actatgttgt cccaattatg aagagacaag gttccggtta tatcttgaat 2160
gtttcttcct acttcggtgg tgaaaagttt ttggcagttg cctatccaaa cagagctgat 2220
tacgcagtct ctaaagccgg tcaaagagca atggttgaat ccttctcaag atttttgggt 2280
ccagaaatcc aagttaatgc aattgctcct ggtcctgttg atggtgacag attgtcaggt 2340
actggtggta aaccaggttt attcttgaga agagccagat taatcttgga aaacaaaaga 2400
ttgaatgcaa tctatgctgc agttatcgaa tcaataagag aaggtgcaga agttacacac 2460
gttttgggta gattagccag aaacgatacc ggttacttgt cacacgacag atccacccct 2520
gacccattga gattattatg tttggaatta gccagagaag gtgacggtat ttgcacatgg 2580
gacagatact tattgacacc tgatattgca ggtagattgt taatgagatt ggcctcagca 2640
ggttacttat tggcacatga agaatggcaa caaagaagtg catcagcaga aggtagaact 2700
ggttggttat tggcattacc acctgacgat atgccatatt tgcctcaaga aagaatcggt 2760
aaagaagcag ataaagtcgg tagaggtgtt ttgagtcaat tacatttggg tgcaatgcca 2820
aaggaatcag aagttgccca agcaacagta ttttacttgg ctgatagagc agtttccggt 2880
gaaacattca tgccttctgg tggtttgtcc gtagaaagat cctacaccga aagagaaatg 2940
tttggttccc ctaaacaaga aagattggaa caaatgaagg gtaaaacagt atgggtcatc 3000
ggtgaacact tagccgatta tatcgccgaa actgcaaatc acttaatcgg tggtttaggt 3060
gttgcaagat tggtcttatt gacaagatct gaagccggtg gtgcttcagt tttggctttg 3120
ttagaccctg aaatcgcaga aagagttacc gtcagattgt gtggtgcaga tgtagaatca 3180
gctatggatc aagctttaac agacttcggt agacctacca cagtagttag tactcctatg 3240
gcaggtttac cagacagatt gtttgaagat ggtgcaatgt tagaaccaga agagtttaga 3300
actttgtgcg aagaaaattt gacacaacac tttagagttt ccagaaaggc atccttatat 3360
gatggttgcc aattggtttt agtatcacca gatgtcccat ttggtcaatc cggtcctgcc 3420
tttgcattag ccaacttcgt caagacaact ttgcatgctt tcaccgcaac tttagctgta 3480
gaaaacgaaa gattagttca tgatgttcct accaatcaaa tcaacttgac cagaagagta 3540
agaagtgaag aaccaagaaa cgctgtagaa caccaagaag aattgaagag atttgctaga 3600
gcagttttgt tggttggtgc acctttgcca gatgcccaag attccagata cagagcaaga 3660
atctacagag gtacaagtat gacagtttaa 3690
<210> 37
<211> 3663
<212> DNA
<213> HPDH-MCRca
<400> 37
atgagtggta caggtagatt ggctggtaaa atagcattga taacaggtgg tgcaggtaat 60
ataggttccg aattgacaag aagatttttg gctgaaggtg caactgttat catatcaggt 120
agaaatagag ctaaattaac tgctttggcc gaaagaatgc aagcagaagc aggtgttcca 180
gctaaaagaa tcgacttaga agtcatggac ggttccgatc ctgtagctgt tagagctggt 240
atagaagcta ttgttgcaag acacggtcaa atcgacatct tggtaaataa tgctggttct 300
gcaggtgctc aaagaagatt ggctgaaatc cctttgactg aagcagaatt gggtccaggt 360
gctgaagaaa ccttgcatgc ttctattgca aacttattgg gtatgggttg gcatttgatg 420
agaatagctg cacctcatat gcctgtaggt tctgccgtaa ttaacgtttc caccatattc 480
tcaagagcag aatactatgg tagaatccca tatgttaccc caaaagcagc cttgaatgca 540
ttgtcacaat tggctgccag agaattgggt gccagaggta tcagagttaa taccatcttt 600
ccaggtccta ttgaatccga tagaatcaga accgtatttc aaagaatgga ccaattgaag 660
ggtagacctg aaggtgacac agcacatcat ttcttgaata ccatgagatt gtgtagagcc 720
aatgaccaag gtgcattaga aagaagattt ccatcagtag gtgacgttgc agatgcagct 780
gtatttttag cttcagctga atctgccgca ttgtctggtg aaaccatcga agtaacacat 840
ggtatggaat tgccagcttg ttctgaaaca tctttgttgg caagaacaga tttgagaact 900
atagatgcat ctggtagaac aaccttaata tgtgctggtg accaaattga agaagttatg 960
gctttgactg gtatgttaag aacttgtggt tctgaagtta ttataggttt cagatccgct 1020
gcagctttgg cacaattcga acaagccgtt aatgaatcta gaagattggc aggtgctgat 1080
tttacccctc caatcgcttt accattggac ccaagagatc ctgctacaat tgatgcagtt 1140
tttgattggg gtgcaggtga aaatactggt ggtatccacg cagctgttat cttgcctgca 1200
acctctcatg aaccagcccc ttgtgttata gaagttgatg acgaaagagt cttgaacttc 1260
ttggctgatg aaatcacagg tactatcgta atcgcttcta gattggcaag atactggcaa 1320
tctcaaagat tgacaccagg tgccagagca agaggtccaa gagtaatttt cttgtcaaac 1380
ggtgctgatc aaaatggtaa tgtatatggt agaatacaat cagctgcaat aggtcaattg 1440
atcagagttt ggagacacga agctgaattg gattatcaaa gagcttctgc tgcaggtgac 1500
catgtattgc ctccagtttg ggctaatcaa atcgtaagat tcgcaaatag atctttagaa 1560
ggtttggaat ttgcctgtgc ttggaccgct caattgttac attctcaaag acacatcaat 1620
gaaatcacct tgaatatccc tgctaacatc tcagcaacca caggtgcaag atccgcttca 1680
gttggttggg ccgaatcttt aataggtttg cacttgggta aagttgcatt gatcacaggt 1740
ggttctgctg gtatcggtgg tcaaatcggt agattattgg cattgtcagg tgcaagagta 1800
atgttagcag caagagacag acacaagtta gaacaaatgc aagccatgat acaaagtgag 1860
ttagccgaag taggttacac tgacgtagaa gatagagtcc atatagcacc aggttgtgat 1920
gtctcctccg aagcccaatt agccgatttg gttgaaagaa ctttgtccgc tttcggtaca 1980
gttgattatt tgattaataa cgccggtatc gctggtgtag aagaaatggt tatagatatg 2040
ccagtcgaag gttggagaca tacattattc gcaaatttga tctccaacta cagtttgatg 2100
agaaagttgg ctcctttgat gaaaaagcaa ggttctggtt acatcttgaa tgtttcttca 2160
tactttggtg gtgaaaaaga tgctgcaatt ccatatccta acagagcaga ctacgccgta 2220
tcaaaggcag gtcaaagagc tatggctgaa gtttttgcta gattcttagg tccagaaatc 2280
caaattaatg caattgcccc aggtcctgtt gaaggtgaca gattgagagg tactggtgaa 2340
agacctggtt tgttcgctag aagagcaaga ttaatcttgg aaaataagag attaaacgaa 2400
ttgcatgccg ctttgatagc agccgctaga acagatgaaa gatctatgca cgaattagta 2460
gaattgttgt tgccaaatga cgttgcagcc ttggaacaaa acccagctgc acctactgct 2520
ttaagagaat tggcaagaag attcagatct gaaggtgacc cagctgcttc ttcttcttct 2580
gcattgttga atagatcaat tgcagccaag ttattggcta gattacataa cggtggttat 2640
gtattgccag ccgatatatt tgctaatttg ccaaacccac cagatccatt tttcacaaga 2700
gcacaaattg atagagaagc cagaaaagtt agagatggta taatgggcat gttgtacttg 2760
caaagaatgc caactgaatt tgatgttgct atggcaacag tctattactt agctgacaga 2820
aatgtttctg gtgaaacttt tcatccatca ggtggtttga gatatgaaag aactcctaca 2880
ggtggtgaat tattcggttt gccatcccct gaaagattag ctgaattggt cggtagtacc 2940
gtatacttaa ttggtgaaca tttgactgaa cacttgaatt tgttggccag agcttatttg 3000
gaaagatacg gtgcaagaca agttgtcatg attgttgaaa ccgaaactgg tgctgaaacc 3060
atgagaagat tattgcatga tcacgtcgaa gccggtagat tgatgactat tgtagctggt 3120
gaccaaatag aagctgcaat cgaccaagct attacaagat atggtagacc aggtcctgta 3180
gtttgtacac cttttagacc attacctacc gtccctttgg taggcagaaa agattctgac 3240
tggtcaaccg ttttatctga agccgaattt gctgaattat gcgaacatca attgactcat 3300
cacttcagag ttgcaagaaa gattgccttg tccgatggtg caagtttagc cttggtcacc 3360
ccagaaacta cagcaacttc caccactgaa caattcgcat tggccaactt catcaagaca 3420
accttgcacg ctttcacagc aaccatcggt gtcgaaagtg aaagaactgc tcaaagaatc 3480
ttaattaacc aagtagattt gacaagaaga gctagagcag aagaaccaag agatccacat 3540
gaaagacaac aagaattaga aagattcatt gaagctgttt tattggtcac agcaccattg 3600
ccacctgaag ccgataccag atacgctggt agaatacata gaggtagagc tataacagtt 3660
taa 3663
<210> 38
<211> 3660
<212> DNA
<213> HPDH-MCRcag
<400> 38
atgtccgtca caggtagatt ggcaggtaaa atagccttga taacaggtgg tgctggtaac 60
ataggttcag aaatgactag aagattctta gccgaaggtg caactgttat tatctcaggt 120
agaaactcag ctaaattggc cgctttagca gaaagattga gatccgaagc aggtgttcct 180
gctaagagaa tagatttgga agttatggac ggttcagatc cagctgctgt tagagcaggt 240
gttgcagcta tcattggtag acatggtcac atagatatct tagttaataa tgcaggttcc 300
accggtgcac aaagaagatt ggctgaaatc ccattaaatg aaaccgatag agacttagac 360
gatgaagaag ctttgtctac aagtgttgca aatttgttgg gtatggcttg gcacttaatg 420
agaatcttaa gtcctcatat gccaccaggt tctgctatta taaacatctc caccatcttc 480
agtagagctg aatactatgg tagaatccct tacgtcgttc caaaggcagc cttgaataca 540
ttgactcaaa tcgctgcaag agaattgggt atcagaggta tcagagttaa cacaatcttt 600
ccaggtccaa ttgaatccga aagaatccaa acagtttttc aaagaatgga ccaattgaaa 660
ggtagaccag aaggtgacac agcatcacaa ttcttggcaa ctatgagatt atacagagct 720
aacgatcaag gtcaattgga aagaagattc ccaacaatct gtgacgttgc tgatgctgca 780
gttttcttgg cctctgatga agcagccgct ttgacaggtg aaactatcga agttacacat 840
ggtatggaat tgccaacatc ttcagaaacc tctttattgg ctagaacaga cttaagaact 900
attgacgcaa atggtagaac cactttgata tgtgccggtg accaaatcga agaagttatg 960
gctttgactg gtatgttgag aacttgtggt tctgaagtca ttatcggttt tagaagtgaa 1020
gctgcattgg cacaattcga acaagccatc ggtgaatcta gaagattggc tggtgaatcc 1080
tttatcccac ctattgcatt accaattgac ttgagaaacc caagtacaat agacgcatta 1140
tttgattggg ctggtgaaaa tactggtggt attcatgcag ccgtaatctt gccagcatca 1200
ggtagagaac ctgcaacaca agttatagac atcgacgatg ctcatgtcca agccttctta 1260
aacgatgaaa tcgtaggttc catcattatc gcatcaagat tggctagata ctggcaagca 1320
caaagaatcg cacctggtgc cagagcaaga gaacctagag tcatcttctt gtctaatggt 1380
gcatcaactg ctggtaatcc ttacggtaga atccaatccg cagctataga acaattaata 1440
agagtttgga gacatgaagc agccttggat tacgaaagag caaccgctgc aggtgaaaga 1500
gttttgcctg cagtttgggc atcacaaatc gttagatttg caaacagatc cttagaaggt 1560
ttggaatttg cctgtgcatg gaccgcacaa ttattacatt cccaaagaag aattaatgaa 1620
ataacattaa ctatccctgc cgacatctct gctactacag gtgctagaag tgccagtgtt 1680
ggttgggctg aaagtttgat aggtttacat ttgggtaaag ttgccttgat tactggtggt 1740
tccgcaggta taggtggtca aataggtaga ttattggctt tgtccggtgc aagagttatg 1800
ttagccgcaa gagatcctca caagttggaa caaattcaag ccacaattag agcagaatta 1860
gcagaagtcg gttataccga tgtcgaagaa agagttcaaa tcgctccagg ttgcgacgtt 1920
tcatccgaag aacaattagt tgacttagtt gaaagaacat tagcagcctt tggtacagtt 1980
gactatttga tcaacaatgc tggtatagct ggtgtagaag aaatggttat cgacatgcca 2040
gttgaaggtt ggagaaacac attgtatgcc aatttgatct caaactactc cttgatgaga 2100
aaattggcac ctttgatgaa gaaacaaggt tcaggttatg ttttgaatgt ttcaagttac 2160
ttcggtggtg aaaaggacgc agcaatccca tacccaaaca gagctgacta tgctgtctca 2220
aaagcaggtc aaagagcaat ggcagaagtt ttcgctagat ttttgggtcc agaaatccaa 2280
attaatgcta tagcacctgg tccagtagaa ggtgacagat taagaggtac aggtgaaaga 2340
cctggtttat ttgcaagaag agccagattg atcttggaaa acaaaagatt aaacgaatta 2400
catgccgcat tgatcacagc agcaagaaca gataatagac caatgagaga attggtagaa 2460
ttattattac ctaacgatgt tgcagcattg gcacaacatc ctgctgcccc agatgttttg 2520
agaaccttgg caaagagatt tcaatccgaa ggtgaccctg ctgcatcatc ctcttctttc 2580
ttattgaaca gaagtatcgc tgcaaaattg ttggcaagat taattaatgg tggttacgat 2640
ttgccagcag atatcttcgc taacttggca gtcccacctg atccattctt tacaagagcc 2700
caaatcgaca gagaagccag aaaagtcaga gatggtataa tgggtatgtt atatttgcaa 2760
agaatgccaa cagaatttga cgttgcaatg gctaccgtat actacttggc agacagaaat 2820
gtttccggtg aaaccttcca tccttctggt ggtttgagat acgaaagaac accaacaggt 2880
ggtgaattgt tcggtttacc tgctccagaa agattagccg aattggttgg ttccactgtc 2940
tatttgatcg gtgaacactt gactgaacac ttaaacttat tggcaagagc ttacttggaa 3000
agatatggtg ccagacaagt tgttatgatc gtagaaaccg aagcaggtgc cgaaaagatg 3060
agacacttat tacatgatca tgttgaagca ggtagattac caatcatagt agcaggtgac 3120
caaattgaag ctgcaatcga ccaagcaata gctaattatg gtagacctgg tccagttgtt 3180
tgtactccat tcagaccatt accttccgcc ccattagttg gtagaaagga ttcagactgg 3240
tccaccgtat tatcagaagc tgaatttgca gaattgtgtg aacatcaatt gactcatcat 3300
tttagagtcg caagaaagat agcattgtcc gatggtgcat cattggcctt agttacacct 3360
gaaactaccg caacatcttc aacagaacaa ttcgccttag ccaacttcgt caagacaact 3420
ttacatgcct tcaccgccac tatcggtgtc gaatcagaaa gaactgcaca aagaattttg 3480
atcaatcaag tagacttaac cagaagagca agagccgaag aaccaagaga tccaagagaa 3540
agacaacaag aattggaaag attcatcgaa gcagttttat tagttactgc tcctttacca 3600
cctgaagcag acactagata cgcaggtaga atacatagag gtagagccat cactgtttaa 3660
<210> 39
<211> 3696
<212> DNA
<213> HPDH-MCRct
<400> 39
atgagaagaa caaaaccaga atgcttgaga agaaatgaaa gtacaatgag attgaacggt 60
aaagtcgcct taataacagg tgccgcaggt ggtatcggtt cagtagtcac aagaatgtac 120
ttgagagaag gtgctagagt tgcaatttcc tcaagatcct tggaaagagc agaacaattc 180
agagcagcct taatggccga aggtttttca ggtgacgata ttttgccagt agcatttgcc 240
agtacagact tagccggtat gcaaacagct atcgaagcaa ttgcagctag atggggtggt 300
ttggatgttt taattaacaa tgctggttca gctggtgcaa agcaaccttt gttcagaatc 360
cctttcacct cagaagattt ggctcaattg gcagctgaag gtttcacaga aaccgaaaca 420
atgagagaaa gtgctgccaa cttattgggt atgccttggc acttaacaag attggcttta 480
ccttacttga gagttggtgc ttctattatc aatgtatcta caatcttctc aagaaccaat 540
tattatggta gaataccata cgtagtccca aaatctgcct tgaacgcttt atccttgggt 600
ttggccaaac aattgggtac cactgacaga gctgtcagag ttaataccgt ttttccaggt 660
ccaatagatt cagacagaat cagaaccgtt ttcggtgcta tggacagatt gaaaggtgtc 720
ccagaaggta ctaccgctca agaatttttc gacattatga ttttggctag agcaagaggt 780
ggtgaagcac ctacaaagtg ttatccaact caagctgatg ttgctcacgt aattttattt 840
ttgggttcag atgaatctgc agccatatct ggtcataact ttgaaataac tcatggtatg 900
caagttaagg cacaatccag aacaaaattg actgcatggc cagaccaaag attgatagat 960
ttgtcaagaa gagttgtctt gattgttggt ggtgaacaag tacatgatgc tgtcacaata 1020
gcagcctccc aacaatcata tggtgcttca gttatcttga cattcagaca accagaagca 1080
gttaatactg caaaagctca attggaatcc agaggtttga accattctat agcagttacc 1140
tggttagacc cattgagaag agaatctgtt acaagtgttt tcgacttaat cagagaaaga 1200
taccacagat tggataccgt tatagttttt ccagctcatg ccgctggtca ttatggtcct 1260
gaattgatcc cagatgaacc tgaaagaatc agacaattca ccgatgaagc tattttaggt 1320
acaaccgcat tcgcagctca attgtccaga tttttattgg ctttcgatgc agaaaacgaa 1380
ttgcaagaac ctgtcaacgt tttgtttgtc acaaacgaac acgacggtgc tacaaatgca 1440
tttgaagata tctatagagc cgcagtcgaa caattgttga gagtttggag acacgaagat 1500
gaattgcaag ttgcagctgg tacaagaaga ttcgccgtaa gaaccaacca aatagtaaga 1560
tatgctaatg cagaaccaga caacttgaag tttacagccg attggttggc aacattagcc 1620
aacagagtca gaatctttga tgaattaaac ttatacgtcc ctgaaaacat acaaagaacc 1680
accggtaaaa ctgaattgcc aagagatatt gccagaactt tattaggttt gcacataggt 1740
aaagttgcag taataacagg tggttcagct ggtataggtg gtgctatagg tcattactta 1800
gctttgtcag gtgcaaaagt agttttggct gcaagagatg ctgaaagatt ggctgcattg 1860
aaaagagaaa tcgttgcaga attatttgcc gttggttacc ctcaaccaga aaccagagtt 1920
gaaaccttgg ctaatataga cgtagctgat gaagaagctt tgggtagatt agtaaaattc 1980
gctttggata aatttggtag aatagacttc ttgataaata acgcaggtat tgctggtgcc 2040
gaagaaatgg ttgtagactt gccattggca gcttggagac atactttgaa agcaaatttg 2100
ttaagtaatt actccttgat atacaagata gcacctttga tgaaaaagca aggtgcaggt 2160
tatatcttga atgtatcatc ctacttcggt ggtgaaaagt acgttgccgt tgcatatcca 2220
aatagatccg attacgctgt ttccaaagct ggtcaaagag cattggctga aatcttggct 2280
agacacttgg gtcctgaaat ccaaattaat gctttatccc caggtcctgt tgaaggtaaa 2340
agattaagag gtgaaggttc tagacctggt ttatacgcca gaagagcaag attgatcttg 2400
gaaaataaga gattgaatga catccatgat gctgttatca gagcatggag agaaggtgaa 2460
gctgttaaat ccaccttggg tgcattggct cataatcatt tcgaagatgt tttagctgca 2520
ggtccaccag aacctgttag aagattaatt acccatttga gagctgcagc tgtcaacgaa 2580
ggtcattcag attcccatta cttgatgacc agaaaaatcg ctgaaaagtt gttgcacaga 2640
ttgttcgaag gtggttactt gacatcagtc gaagatagag aagcttttac cttagctttc 2700
ttgagagaat taccagaacc tccagatcca tttttcgatc caaaagaaat tgatagagaa 2760
gctgaaagag ttcaatctgg tatcttgact atgttgaatt tgagaagaat gcctacagaa 2820
gaagaaatcg ctatcgctac agttttctac ttgtctgatc ctaacatttc tggtgaaacc 2880
ttgcacccat caggtggttt gaaattcgac agaacagtaa ccgaaggtga attttatggt 2940
ttaccaagaa atgaacattt gatggaatta agaagaaaga atgtattgtt aatcggtgac 3000
tacttggaag aagaattgag atggttggtc ggtgctttcg caatccaatt agttgtaaga 3060
aattgtatca ttttgacaaa gaccgccgaa agagctgata gtttgagaca ttacttttct 3120
gcacaaagat tgaacaattt gcatatcttc tctgctggtg caaacgttga agccgctatg 3180
gatgacttga taagaagatt tggtcacttg gatgtcgtag tatccacacc ttttgaccct 3240
ttaccattga aacctttggc tgcaactgat gattggagag gtgtcttgaa tgaaaaagac 3300
ttcgaacaat tggttgaaac acatattact catcacttca gagtcgccaa aaaggctgcc 3360
ttgcaagata aatcccaaat cgtattagta acccctaaga catcaagaaa tagttccaga 3420
gaagaatttg ctttagcatt gttcgttaaa acaaccttac atgcattgac agctaccttg 3480
gcagttgaat gcgaaagatt cacccatcat cctactgtaa atcaagttga tttgacaaga 3540
agagctagag tcgaagaacc aagaaacgaa agagaagaaa gagaagaatt ggaaagattc 3600
gtagacgctg ttttattggc tgccgcacca gctcctgaac cagaacaatc cagatacttg 3660
tccagaatac atagaggtaa cgccatcact gtttaa 3696
<210> 40
<211> 3651
<212> DNA
<213> HPDH-MCRgb
<400> 40
atgaacgata tgtccgatat tccacaagac ttattaacac aaagtatagc aggtagaaga 60
ttagataaca aatgtattat tttaactggt gcagctggtt caatcggttc ctttattaca 120
agacaattat tgagagaagg tgcaagagtt atgttaacag gtagagatca atcaaaattg 180
gacgacttta ttggtgactt ggtatcagaa ggtttcgaag aaactttcat ggtttccgca 240
actggtgact gtgccgatcc tgaagtctgt agagaaatag ttgccaagac agttgccgca 300
ttcggtccta ttgatgtttt agtcaacaat gccggtgccg caggtccaaa attcacattg 360
agagatatcc ctttcaccga agcagaacaa agagcagctg actcagatca aacaatgttt 420
gactcagcca tgaatttgtt gggtgctcct tggaatatgg ccagagccgc tgcaccacat 480
atgtcagctg gtgcctctat aatcaacgtt agtactatct tttctagaac tcattactat 540
ggtagaatac cttacgtcgt tccaaagtct ggtttgaacg cattgggtaa aggtttggca 600
ttagaattgg gtgaaaaaca aggtatcaga gtaaacactt tatttccagg tcctattgaa 660
tccgaaagaa ttgaatcagt tttcgccaga atggatgaat tgcaaggtgt tgaacctggt 720
tcaactggta aagagtttag agatttgatg ataactacaa gaacctctga tggtggtttg 780
gaatataaat acccaactcc aacagatgtt gcatcaggta tcgtttggtt ggcatctgat 840
gaatcctcag ccttatctgg tcatcacatt gaagtcacca atggtatgca agtaccagca 900
caatctagat cccaattggt ttcatggcca gataagagat tggaagattt gtcaggtcaa 960
gtcgttttga ttttaggtgg tgacgactat caagaagcag ttactttcgc cgaaagacaa 1020
atccaatccg gtgcaagagt aatgttagct ttcagaaact tgaataccgt tggtcatgct 1080
agatcattag ttcaatcaag aaatttgggt gacgttcaat tatcccactt ggaccctttg 1140
agaagagaat cagttgacag atccatgcaa ttaatcgcag atcactttgg tagattagat 1200
ggtgtaatct tgttaccaca aaagccaaac ggtgaatacg gttattcctt gactacagct 1260
aatgacgaag atgttgccaa ttttgttaga gatgaagttg tcgcccctgt tgcatttgca 1320
tccgctttcg caagaaacat ggatcaattg tttgccgaag gtgaaccacc agccatcgtt 1380
tacgttacta accctagtga tagacatggt aacttgatga atgaaattat cagagcaagt 1440
gttgaagcct taatcagagg ttggagacat gaagatgaaa ctttggcaaa cactggtgaa 1500
ttgccttggg cagtacaacc aaaccaaatg gttagatatg ataccgaaga tagagaagct 1560
ttaactttcg cagccgactg ggcagccact ttaaccaaca gagttagaaa gatggatagt 1620
atcaacttat ggttaccaag aagtattaaa agatccactg gtaaaagtag tatgccaagt 1680
agtataagta gagtattgcc tggtttgcac aagggtagaa cagccgtaat cactggtggt 1740
tccttaggta ttggtttaca attgggtaga tttttggcaa tagctggtgc cagagtcttg 1800
ttatctgcca gaagtgaagg taaattggcc gaagcaagag atgaaatcgt tgacgaattg 1860
agaaacatag gttatcctag accagaatct agagttacaa ttttgggtgg tatcgacgta 1920
ggtgaccctg atgcattgga tagattacac gatcatgccg ttgcagaatt gggtcacgta 1980
gatttcttga ttaataacgc tggtatcagt ggtgccgaag aaatggtagt cgatatgacc 2040
agagcagact gggatagaac tatggaagcc aacttgatct ctaactactc attgattaga 2100
aaattctcac ctgccatgaa agcaggtggt aaaggttcca tattgaatgt ttcctcatac 2160
tttggtggtg aaaagtatgt tgccgtagca tatccaaaca gagctgatta cgccgtatct 2220
aaggcaggtc aaagagtatt agcagaaata ttgtcaagac acttgggtcc tgaaatccaa 2280
atcaacgctt tagcacctgg tccagttgat ggtgccagat tgagaggttc agccgaagcc 2340
cctggtttat tcgatagaag aggtagatta gttttagaaa ataagagatt aaatgaagtc 2400
cataaggcta tcttggctgg tatgagttca ggttttgatg ctgacggtgt tatgagtttg 2460
gcatcaaacg ctgttgattc aattgacatc gactccttac ctaaaccagt cgccagattg 2520
gtcttgaaaa ttagagattc tggtggtatg ggtaactcct cacactactt aatgcacatg 2580
ggtatcgccg caaaattgat gactagatta gttagagcag gtatcttgtc cgacgaacaa 2640
agagaacaat ttttgggtac attcatagac gccccagcac catttttcga tttctcagaa 2700
acttccaagc aagcagaaca aatcgaaaca ggtattttga acagattaca cttgcataag 2760
atgcctacag atgaacaagt cggtttatcc accgtctttc acttggcaga tgacattgta 2820
tctggtgaaa cctttcatcc atcaggtggt ttgaaattcg acagatccgt cacagaaggt 2880
gaattgttgt tacctccaaa tgaaacagaa gttgctaagt taaagggtaa aagaatcgtt 2940
ttgattggtg actcaatgag aaacgaattg actcatatag ctaatggttt cttagcacaa 3000
aatgtagaaa aattgtgggt tttgactaag acagaagatt ccgcaaacgc tatgactcat 3060
tcagtcgata accctcatgg tatcgacata gaatgtagag ctattggtga cgacttagaa 3120
agttcattag atgacatctt gagaaacgat ggtggttatg acgttgtagt ttcatcccca 3180
ttcgaaagat tgccattaaa tgcattagct gccaacgcta atgaaccttg ggatagagtc 3240
ttatccgacc atgagtttag aaagttagtt cacgaccaat tgacccatca ctttagagtc 3300
gcaagaacca gtgccttggt tcctaattgc caaatcgttt taattactcc tgacacttct 3360
ttggcatcaa ctagagaaga atttgctttg gccttattcg ttaagaactc attgcacgct 3420
tttacagtca cattgggtgt agaaggtgaa agattgccaa cagttccagc aattaatcaa 3480
gtccaattga ccagaagagc acataccgaa gaaccatcca acgaccaaga attggaagaa 3540
gaaatgacaa gattagtcca tgccgtatta caatgctcag tccctgcacc agcaccatcc 3600
gattcaagat acttgtccaa aatctttaga ggtaacgctg tcaccgtata a 3651
<210> 41
<211> 3666
<212> DNA
<213> HPDH-MCRot
<400> 41
atgtttatga caagattgaa tgacaagatt gctttgatta ctggtggtgc tggtactata 60
ggtgaagtta ttacaagaag atatttggaa gaaggtgcaa cagtagttat ggctggtaga 120
aatagagaca aattagacag atatagagaa agattgataa ctgaatttca tgctttacca 180
gaaagagtta tggtagttag aatggacggt tcatccaacg cagaagttag aatgggtatt 240
gccgctgttg tagcccactt tggtagaatt gatatattgg taaataacgc aggttcagct 300
ggtgccagac aaagattgcc agctatccca ttattgagat cagaattgca agcagacgaa 360
accgaaactt tggcagattc aatcggtaac ttaataggta tcacatggaa tttgattaga 420
gctgcagctc cattcatgcc tgcaggttcc tcagttatca atattagtac aatctttgct 480
agaactgact actatggtag aatcccttat gttgtaccaa aggccgcatt acacgcctta 540
acattggcag ccgcaactga attaggtgaa agaggtataa gagttaacca aatcaaccca 600
ggtcctatcg actcagatag aattagaact gtttttagaa gaatggatga attgaagggt 660
gttccagaac aatcaactgc tgatggtttc tttcaaatga tgagattgag aaggcctaat 720
gccgaaggtg acttagttaa gggttttcct aagactttgg atgttgccaa cgttgctgta 780
tttttaggtt ccgcagaatc agctgcattg tccggtgaaa cattggatgt tacacacggt 840
atggcagttc caacagaatc aagaacaact ttgacaagta gacctggttt gagagcagta 900
gacggtagtg gtcacacaac attaatttgt gttggtgacc aaatcgaaga agcagcagcc 960
ttaactggtg tattgagagc atgtggtgcc gaagtagtta tcggttttag atctagagcc 1020
gcaatcgcta gattcgacca cttgatcgaa agaggtagac acttaccatc tcaagaacat 1080
gttgcccctg ttttgttata tttgaatcct acagaaccag aatcaataga ccaagccttg 1140
agatggatgg ccactaactt agacttgcct acaagtgtaa tcattttgcc tgcacaaaga 1200
caaccattac ctcctagtgt tgttagagcc tcagatgaag aagttgcata ctttttaaga 1260
gatgaattga gtggtatgat cgtcttggca agtagattag caagattctg gcaacaagca 1320
acattggcac ctggtaatgc acctattcaa ccaagagttt tattcatgac aaacccagac 1380
gatggtcaag gtaacttata tgcagaaata ttgagagcag gtgttgaaca attgtgtaga 1440
gtttggagac acgaatccca attggattat actagattgg cccaaatgga tgcacatcca 1500
cctcatatta gaccagtttg ggcaaaccaa ttggttagat ttgccaacaa tgaacaagaa 1560
aatttggaat actgctgtgc ttgggttgcc aagatattgt tatccgaaag aacaattgaa 1620
gaattaaact tatacttgcc tagacaaata ggtagtacaa caggttccag acaaccttcc 1680
tttggttggg cagaaaactt gataggtttg cacttgggta aaacagcatt gataactggt 1740
ggttcagctg gtataggttc ccaaatcgca agattgttag cattgtcagg tgcaagagtt 1800
atgttgtgcg caagagatga aagaaaatta atccaaatga gagacatgat aatcgcagaa 1860
ttgactgaag tcggttataa ccaagttgaa tctagagtac aaatttgtgc cggttgtgac 1920
gttggtgaag aagaacaatt ggaaattgct gttcaaagaa ctttggactt atttggtcat 1980
gttgattact taattaataa cgcaggtatt gccggtgctg aagaaatggt tttagacttg 2040
cctttagaag cttggcaaag aactttaaga acaaatttga taagtaatta ttcattgatc 2100
agaaaattgg ctccacaaat gaaatcaaga ggtagtggtt atatcttgaa tgtctcttcc 2160
tacttcggtg gtgaaaagta tgccgctatt ccttatccta atagagctga ttacgctgta 2220
tccaaagccg gtcaaagagc cttaggtgaa gctttggcta gattgttagg tccagaagtt 2280
caaatcaacg caatggctcc aggtcctgtt gaaggtgaaa gattgagagg ttctggtgac 2340
agacctggtt tatttttaag aagaggtaga ttaattttgg aaaacaaaag attaaacgac 2400
ttgcacgcaa cattgattgc tgcagaaaga gaaacccaag ttggtatgag agacttatta 2460
gccagattgt tacataacga tgtttgtgct ttgattgatg accctgcagc cccaacacac 2520
ttgagagctt tggctgaaag aatctgggaa caatccgatc caaactccta tgctagagcc 2580
ttctttatga atgccaatat tgctactaag ttattggcca gattgttcaa cgccgatcaa 2640
attgacgccc aaacattcca cacatcccaa cctaacttgc cacctgaacc tttcttcgct 2700
agaacccaaa tagatagaga agctagaaga gtcagagatg gtgtaatgtc catgttgtac 2760
ttacaaagaa tgcctacaga atttgacgta gcattagcca cagtttatta cttgaacgac 2820
agatccgttt ctggtgaaac attccatcca agtggtggtt taagacatga aagaacccca 2880
accggtgccg aattgtatgg tagtcctgca ccacaaagat tggccagttt agccggttca 2940
accgtctatt tgattggtga atcaatggct gctcacttgg aagctttagc aagagcttac 3000
attgaaagat acgcagctac aagagttgtc ttgatctgtg ctacacctgc aggtgttgaa 3060
agattcagtc atcacttagc cgaccactta gcttctggtg ctttggccat attatccgcc 3120
gaagaaggta tcgaagcagc tttgtcagaa gccttgagaa gattcggtcc tccaggtcca 3180
gttgttagta ctcctttcca acctttgcca tcacaacctt tgattggtag aaacgactca 3240
gattggagta ccgttttaga tgttgctggt ttctccgcaa tgtgtgaaca acaattaacc 3300
catcacttta gagtcactag aaaattaagt ttggtcgcag gtgtatcatt ggttttggtc 3360
actcctgaaa cagattcaca cagttccaca gaacaattcg ctttggctaa ctttgttaag 3420
actacattac acgctttcac tgccacagtt ggtgttgaat gcgaaagaac agcacacaga 3480
attttggtca atcaagttga tttgggtaga caagctagag cagaagaacc aagatcacct 3540
gcagaacaag cacaagaaat ggaaagattc attgacgcca taatgttaac taccgcacct 3600
attccagctg aagaagataa cagatatact ggtagaatat acagaggtag agccataaca 3660
gtttga 3666
<210> 42
<211> 3690
<212> DNA
<213> HPDH-MCRrc
<400> 42
atgtctacag tcagaagatt ggaaggtaaa gttgcattga taacaggtgg tgccggtaat 60
ataggtgaag taataacaag aagatttttg gcagaaggtg ctaccgttgt tatcacaggt 120
agaaatgccg aaaaattggc tgtctacaga agaagattaa ttgacgaaga aagagtagca 180
ccagaaagag tcgttgcttt gagaatggat ggttctgaca ttgcccaagt tagagctggt 240
gttgctcaaa ttgttcacgg tggtacagat gtcccaatcc cattgcatag aatagatatc 300
ttggtcaaca atgcaggttc tgcaggtcca agaagaagat tagttgatat ccctttggaa 360
ccatccgaag ttcaacctcc agattcagaa actttggcac aagctgttgg taatttggtt 420
ggtataacat ggaatttgac aagagcagcc gcaccacaca tgccatccgg ttcatctgtt 480
attaacatct ctacaatctt ctcaagaacc gattactacg gtagaatagc ctatgtagca 540
cctaaggctg ctttgaacgc attatctgac ggtttggcca gagaattggg tgttagaggt 600
atcagagtca ataccatata tcctggtcca atcgaatcag aaagaatcta cactatgttt 660
caagctatgg acgctttaaa gggtcaacca gaaggtgaca cagctagtgg tttcttaaga 720
atgatgagat tgtctagaat cgaccaaaac ggtgaagttg ttaagagatt cccaagtcct 780
gtagatgttg caaatactgc tgtattcttg gcatctgacg aaagtgcagc ttttacaggt 840
catgcctttg aagtcacaca cggtatggaa gttccaaccg aaagtagaac cactttcgtt 900
tccagaccag gtttaagatc cgttgatgca accggtaaag ttattttaat atgtgccggt 960
gaccaagttg atgacgctgt cgcattggct gatacattga gatcatgtag agctaccgtt 1020
gtcatcggtt tcagagatcc aagagcatta gaaaaggcat ccgttttgtt gagagaacct 1080
agacacgctt tggcagctga catgtacggt agacctacaa tgactgcaga agctagattg 1140
gtcagattag atcctttaga cccaagagca gctgcccaaa ctttggaaca aatacatgcc 1200
gaattgggtg ccatccacca tgctgtcgta ttgcctggtc aatctagaca tgcaccttca 1260
gcttccttga tcgaagttga tgaccaagtt gttgaaagat tcttacacca agaattagtt 1320
ggtactattg ccttagcaag agaattggcc agattttggg aagaatatcc atccggttct 1380
tcaatgcata gagttttatt tgtttccaat cctgacgatc aacaaggtaa ccaatacagt 1440
cacatcttga gagctgcagt tgaacaatta gtcagagtat ggagacacga atccgaatat 1500
gactccgtta accctgcaca ccaacaagaa ggtcaatcct cagcagctgt ttgggctaac 1560
caattaataa gatatgttaa taacgaaatg gctaacttgg acttcacttg cgcctgggtt 1620
gccaagttat taggttccga tagaagaatc gccgaaatta acttatactt gcctgaagaa 1680
atcgttggta caataggtgt ccacaatcct ggttttggtt gggctgaatc cttatttggt 1740
ttacacatgg gtaaagtcgc attaataaca ggtggttccg ccggtatcgg tggtcaaatt 1800
ggtagattgt tagcattaag tggtgctcat gttatgttag cagctagaaa tgcagatcaa 1860
ttagaacaaa tgagagcttc catagttaga gaagttagag atgcaagtta tcctgacgca 1920
gaatcaagag ttgccatctt ccctggttca gacgtttcag atatcgacgg tttggaaaga 1980
ttggtaaatc atacagtaag agtttttggt aaagttgact atttgatcaa caatgctggt 2040
atagcaggtg cagaagaaat ggttatagac atgccagttg atgcatggag acacacattg 2100
agagccaact taatctccaa ttatgcatta ttaagaagat tagcaccaca aatgaaggca 2160
gccggtggtg catacgtatt gaacgtatca tcctattttg gtggtgaaaa atacgttgcc 2220
ataccatacc ctaatagatc cgactacgca gtttccaaag ctggtcaaag agcaatggtt 2280
gaatccttgg caagattttt gggtccagaa atacaaatca acgcaatagc tcctggtcca 2340
gttgaaggtg aaagattgaa aggtgctggt tcaagacctg gtttattcat gagaagagcc 2400
agattgatct tagaaaataa gagattgaat gaagttttcg ccgctttatt ggccgcaaga 2460
catgaaggtg ccacaatagc cgacttgtta cctgacttgt tcgccaatga tatccaatca 2520
atagcaaatt ccgcagccat gccagcacct ttgagaagat tagccacaat gttgagagaa 2580
acatctgacg caggtggtag tgcccaatca tatttgatga acgctactat cgcaagaaaa 2640
ttattgaaca gattggaaaa cggtggttat atcaccttac acgatagaag agccttgact 2700
gttgaaccac ctgaaccttt cttcacagaa gcacaaatcg aaagagaagc catcaaagtt 2760
agagatggta tcttgggtat gttacactta caaagaatgc caaccgaatt tgatgtagca 2820
ttagcaaccg ttttctactt ggccgataga aatgtcacag gtgaaacttt tcatccatca 2880
ggtggtttga gatttgaaag aacagttacc gaaggtgaat tgttcggtaa accaggtcaa 2940
caaagattag aaagattgaa gggttctgta gtttacttaa tcggtgaaca tttgagacaa 3000
cacttggttt tattggccag aacctttttg gatgaaatac atgtagccag agttgtttta 3060
ttgaccgaaa ccacacaagc agctactgac ttagcagccg aattgtctga ctatgaagct 3120
gcaggtagat ttgtcgtaat cccaacttgc ggtgacatag aaggtggtat tgacagagca 3180
atggcagaat atggtagacc tggtccagtc atctcaacac ctttcagacc tttaccagat 3240
agagctttat cagctagaaa tggtgactgg tcctcagttt taactacagc tgaatttgaa 3300
gaattggttg aacaacaaat cacacatcac ttcagagttg caagaaaagc aggtttgatc 3360
gaaggtgcta acgttacctt agtcacccca cctacttcag caagatctac atccgaagaa 3420
tttgccttgg ctaattttgt aaaaacaaca ttacatgcat tgaccgccac agctggtgca 3480
gaatccgaaa gaacagttcc acatgtccca gtaaatcaag ttgatttgac cagaagagct 3540
agatccgaag aaccaagaac cccttccgaa gaagaagaag aattgcaaag atttgtcaat 3600
gccgttttgt taacctccgc tcctttacca acacctttgg aatcaagata tagagccaga 3660
atctacagag gtaatgctat cacagtttaa 3690
<210> 43
<211> 3615
<212> DNA
<213> HPDH-MCRsl
<400> 43
atgaatgcaa tgggtaaaac agaaacacaa ggtagattag ctggtaaagt agccttgata 60
actggtgccg ccggtaactt aggtggttct atcgtaagaa gatatttggc cgaaggtgca 120
acagttgtca tgtccggtag agatgctgcc agaactcaag cagctttaga tgcagcagta 180
gctgacgttg gtggtactgc ttcaattgtc actatggatg gtgcaaatat cgaatccgtt 240
agagcaggta tcgcagatgt tgtcgcaaga catggtagaa tagacgtctt ggttaataac 300
gcaggttcag ctggtccaaa gcaagttttg ggtgacttac ctttgttgcc agaagaattg 360
gaaggtacac ctgattcaga aacagttggt gacgcagcaa gaaatataat ggttgtcgca 420
tggaacttag ttagagctgc cgcatctgct atgggtgaag gtggttccat cattaatgtt 480
tccacaatat tctcaagaac tgattatttt ggtagagcag cttatacagt tccaaaagct 540
gcattgaata tcttatccaa aggtatcgca agagaattgg gtaccagagg tatcagagtc 600
aatcaattgt atcctggtcc aatcgcctca gaaagaatca gaacagtttt cgccgcaatg 660
gataagttga aaggtgacga tgccggttca actgccaacc atttctttaa tttgatggct 720
ttggaaagaa gtgtaggtga agatgccaaa gcaaagacct tccctacacc agctgatatc 780
gcaaacactt gcgttttctt gggttcagac gaatccgctg cctttgcagc acacgacttt 840
gaagttacac atggtatggc agtacctttg gaatcaaaat ccgaattttt gcatagacct 900
gacttgagag caatagatgg tgacggtcat ggtatcttga tttcagccgg taaccaatta 960
gacgatgcat tggctatggc aggtttgttc gcagcacaag gttcaaaagt cttgttgggt 1020
tttcacgcac aagctgcatt ggacgatgca gcagaaagat tgccagccgg tgtcaaagca 1080
gttagatttc caagacatga ccatatggca atggacgctg aattggccag attcacagca 1140
catgtagccg caattgacgg tgctattata ttgccaacaa gaggtccagg ttactttcat 1200
ggtgcattga acgaagcttc cgacgctgcc gttgaaagat ttgtcgatga cgaattggaa 1260
ggtgcaattg ctgttgcaag aacattagat agatattggc aacaaggtca agacttgcct 1320
ggtgacccta gagtcttgtt tatcacaaac cctgatgatg gtgcaggtaa tggttgggca 1380
gatgttggta gagcagcaat cgaagaattg ttgagagttt ggagagatga agctagagta 1440
gccgcaacac aaggtaaaac tcctagacct gtttggggta accaaattgt tagatttggt 1500
aataacgaac cagaaaatag agactttgct gcaggtcatg ccgcaagatt gttattaggt 1560
ggtagacaaa tagaaccaat caatttgtac atccctgcat ctatcacaaa agccactggt 1620
gccagaagag ccttagcagg taccgcagaa aatatcacag gtttgcattt gggtaaagta 1680
gccttgatta caggtggttc cgcaggtata ggtggtgcct tagccagatt gttagccttg 1740
gcaggtgcaa aagttatgat ggtcgctaga agagaatctg aattagatgc tgctagagaa 1800
agaatcgttg gtgaattgca agacatcggt ttcgcaggtg tcgaaagaag agttttgacc 1860
ttggctggta ttgatgttgc tgacatgaaa tcattaaagc acgctgttga tgaaacagtc 1920
agagcattcg gtagaatcga ctacttaatt aataacgccg gtgtatcagg tgcagaagaa 1980
atggctgttg atatgggtgt tgaaaacttc agacatacac aatttgcaaa tttgatctca 2040
aactacgctt taatgcacat ggttgtccca atgatgagaa aacaaggttc cggttatatc 2100
ttgaatgtta gttcttactt tggtggtgaa aagtatttgg ctgtctccta tccaaataga 2160
acagactacg cagtatcaaa gtccggtcaa agagcaatgg ttgaaagtat ggcaagacac 2220
ttaggtcctg aaattcaaat caatgccata gctccaggtc cagtagatgg tgacagatta 2280
gctggtttgg gtggtaaacc aggtttgttt gaaagaagag gtagattgat tttagaaaac 2340
aagagattga acgctgttca ctcagctgtt atcaagtccg tcagagcagg tggttctgtt 2400
gcccaaattt tattcagatt gtccagaaat gactcagttc aattgtccca cgataagtca 2460
aacccacaac ctttaagaga tttggccttg gcatgtgcaa gagaaggtga cgaatcatgc 2520
tgctgggaca gattcatctt gacacctgta ttggccgcaa agttgttgaa aagattaaga 2580
ttgggtggtt ggttgatagg taccgactgg caatccagag atgaatccga ttggttgtta 2640
agagtccctc cagacgacat gccttggttg ccagttgcac aaattggtag agaagcagcc 2700
aaagttagag gtggtgtatt gtcccaatta catttgggta aaatgcctac cgaaacagaa 2760
gttgctcaag caactgtttt ctttttagct gatagagcag tctcaggtga aactttcatg 2820
ccttcaggtg gtttaaatgt cgaaagatcc gccaccgaaa gagaattgtt tggttcccct 2880
aaggcagaaa gattggcaca aatgggtggt aaaacagttt ggttggtcgg tgaacatttg 2940
gcagactact tgggtgctgc agctcaaggt ttcttgaatt gtgacgttgc tagagtagtt 3000
ttaatcactg cctcagctgc aggtgctaca gccattaagg gtcatattgg ttccggtgct 3060
gacagagtag aaagtttatt gatagacggt gaaatcgaaa cagccttaga tgcagctttg 3120
gctacttggg gtcatcctac tacaatcgtt tccactccat tgaaagcatt gccagaccaa 3180
ttgtttgatg cagataatcc attagatggt gacggttttg ctgatgtcgt tgaacataat 3240
ttgactcatc actttagaat tgccagaaaa gtcagtttat ttgacgaatg ccaattggta 3300
ttagtttcac cagatgttcc tatgggtgaa ggtgcaaaag gtgcatttgc cttggccaac 3360
tttgtcaaaa ccacattaca cgcttttact gcaactatcg cagttgaaaa tgaaagattg 3420
gtccacgaag tcgccgttaa tcaaatcaac ttgacaagaa gagtaagatc agaagaacct 3480
aaaaacgcag ctgaacatga agaagaagtt agaagatttg caagagccgt tttattggct 3540
ggtgcaccat tacctgacgc agaagatagt agatatagat ccagaatcta cagaggtatg 3600
tccttaaccg tataa 3615
<210> 44
<211> 3663
<212> DNA
<213> HPDH-MCRca_变体_3
<400> 44
atgagtggta caggtagatt ggctggtaaa atagcattga taacaggtgg tgcaggtaat 60
ataggttccg aattgacaag aagatttttg gctgaaggtg caactgttat catatcaggt 120
agaaatagag ctaaattaac tgctttggcc gaaagaatgc aagcagaagc aggtgttcca 180
gctaaaagaa tcgacttaga agtcatggac ggttccgatc ctgtagctgt tagagctggt 240
atagaagcta ttgttgcaag acacggtcaa atcgacatct tggtaaataa tgctggttct 300
gcaggtgctc aaagaagatt ggctgaaatc cctttgactg aagcagaatt gggtccaggt 360
gctgaagaaa ccttgcatgc ttctattgca aacttattgg gtatgggttg gcatttgatg 420
agaatagctg cacctcatat gcctgtaggt tctgccgtaa ttaacgtttc caccatattc 480
tcaagagcag aatactatgg tagaatccca tatgttaccc caaaagcagc cttgaatgca 540
ttgtcacaat tggctgccag agaattgggt gccagaggta tcagagttaa taccatcttt 600
ccaggtccta ttgaatccga tagaatcaga accgtatttc aaagaatgga ccaattgaag 660
ggtagacctg aaggtgacac agcacatcat ttcttgaata ccatgagatt gtgtagagcc 720
aatgaccaag gtgcattaga aagaagattt ccatcagtag gtgacgttgc agatgcagct 780
gtatttttag cttcagctga atctgccgca ttgtctggtg aaaccatcga agtaacacat 840
ggtatggaat tgccagcttg ttctgaaaca tctttgttgg caagaacaga tttgagaact 900
atagatgcat ctggtagaac aaccttaata tgtgctggtg accaaattga agaagttatg 960
gctttgactg gtatgttaag aacttgtggt tctgaagtta ttataggttt cagatccgct 1020
gcagctttgg cacaattcga acaagccgtt aatgaatcta gaagattggc aggtgctgat 1080
tttacccctc caatcgcttt accattggac ccaagagatc ctgctacaat tgatgcagtt 1140
tttgattggg gtgcaggtga aaatactggt ggtatccacg cagctgttat cttgcctgca 1200
acctctcatg aaccagcccc ttgtgttata gaagttgatg acgaaagagt cttgaacttc 1260
ttggctgatg aaatcacagg tactatcgta atcgcttcta gattggcaag atactggcaa 1320
tctcaaagat tgacaccagg tgccagagca agaggtccaa gagtaatttt cttgtcaaac 1380
ggtgctgatc aaaatggtaa tgtatatggt agaatacaat cagctgcaat aggtcaattg 1440
atcagagttt ggagacacga agctgaattg gattatcaaa gagcttctgc tgcaggtgac 1500
catgtattgc ctccagtttg ggctaatcaa atcgtaagat tcgcaaatag atctttagaa 1560
ggtttggaat ttgcctgtgc ttggaccgct caattgttac attctcaaag acacatcaat 1620
gaaatcacct tgaatatccc tgctaacatc tcagcaacca caggtgcaag atccgcttca 1680
gttggttggg ccgaatcttt aataggtttg cacttgggta aagttgcatt gatcacaggt 1740
ggttctgctg gtatcggtgg tcaaatcggt agattattgg cattgtcagg tgcaagagta 1800
atgttagcag atattcaaga agataagtta gaacaaatgc aagccatgat acaaagtgag 1860
ttagccgaag taggttacac tgacgtagaa gatagagtcc atatagcacc aggttgtgat 1920
gtctcctccg aagcccaatt agccgatttg gttgaaagaa ctttgtccgc tttcggtaca 1980
gttgattatt tgattaataa cgccggtatc gctggtgtag aagaaatggt tatagatatg 2040
ccagtcgaag gttggagaca tacattattc gcaaatttga tctccaacta cagtttgatg 2100
agaaagttgg ctcctttgat gaaaaagcaa ggttctggtt acatcttgaa tgtttcttca 2160
tactttggtg gtgaaaaaga tgctgcaatt ccatatccta acagagcaga ctacgccgta 2220
tcaaaggcag gtcaaagagc tatggctgaa gtttttgcta gattcttagg tccagaaatc 2280
caaattaatg caattgcccc aggtcctgtt gaaggtgaca gattgagagg tactggtgaa 2340
agacctggtt tgttcgctag aagagcaaga ttaatcttgg aaaataagag attaaacgaa 2400
ttgcatgccg ctttgatagc agccgctaga acagatgaaa gatctatgca cgaattagta 2460
gaattgttgt tgccaaatga cgttgcagcc ttggaacaaa acccagctgc acctactgct 2520
ttaagagaat tggcaagaag attcagatct gaaggtgacc cagctgcttc ttcttcttct 2580
gcattgttga atagatcaat tgcagccaag ttattggcta gattacataa cggtggttat 2640
gtattgccag ccgatatatt tgctaatttg ccaaacccac cagatccatt tttcacaaga 2700
gcacaaattg atagagaagc cagaaaagtt agagatggta taatgggcat gttgtacttg 2760
caaagaatgc caactgaatt tgatgttgct atggcaacag tctattactt agctgacaga 2820
aatgtttctg gtgaaacttt tcatccatca ggtggtttga gatatgaaag aactcctaca 2880
ggtggtgaat tattcggttt gccatcccct gaaagattag ctgaattggt cggtagtacc 2940
gtatacttaa ttggtgaaca tttgactgaa cacttgaatt tgttggccag agcttatttg 3000
gaaagatacg gtgcaagaca agttgtcatg attgttgaaa ccgaaactgg tgctgaaacc 3060
atgagaagat tattgcatga tcacgtcgaa gccggtagat tgatgactat tgtagctggt 3120
gaccaaatag aagctgcaat cgaccaagct attacaagat atggtagacc aggtcctgta 3180
gtttgtacac cttttagacc attacctacc gtccctttgg taggcagaaa agattctgac 3240
tggtcaaccg ttttatctga agccgaattt gctgaattat gcgaacatca attgactcat 3300
cacttcagag ttgcaagaaa gattgccttg tccgatggtg caagtttagc cttggtcacc 3360
ccagaaacta cagcaacttc caccactgaa caattcgcat tggccaactt catcaagaca 3420
accttgcacg ctttcacagc aaccatcggt gtcgaaagtg aaagaactgc tcaaagaatc 3480
ttaattaacc aagtagattt gacaagaaga gctagagcag aagaaccaag agatccacat 3540
gaaagacaac aagaattaga aagattcatt gaagctgttt tattggtcac agcaccattg 3600
ccacctgaag ccgataccag atacgctggt agaatacata gaggtagagc tataacagtt 3660
taa 3663
<210> 45
<211> 3663
<212> DNA
<213> HPDH-MCRca_变体_6
<400> 45
atgagtggta caggtagatt ggctggtaaa atagcattga taacaggtgg tgcaggtaat 60
ataggttccg aattgacaag aagatttttg gctgaaggtg caactgttat catatcaggt 120
agaaatagag ctaaattaac tgctttggcc gaaagaatgc aagcagaagc aggtgttcca 180
gctaaaagaa tcgacttaga agtcatggac ggttccgatc ctgtagctgt tagagctggt 240
atagaagcta ttgttgcaag acacggtcaa atcgacatct tggtaaataa tgctggttct 300
gcaggtgctc aaagaagatt ggctgaaatc cctttgactg aagcagaatt gggtccaggt 360
gctgaagaaa ccttgcatgc ttctattgca aacttattgg gtatgggttg gcatttgatg 420
agaatagctg cacctcatat gcctgtaggt tctgccgtaa ttaacgtttc caccatattc 480
tcaagagcag aatactatgg tagaatccca tatgttaccc caaaagcagc cttgaatgca 540
ttgtcacaat tggctgccag agaattgggt gccagaggta tcagagttaa taccatcttt 600
ccaggtccta ttgaatccga tagaatcaga accgtatttc aaagaatgga ccaattgaag 660
ggtagacctg aaggtgacac agcacatcat ttcttgaata ccatgagatt gtgtagagcc 720
aatgaccaag gtgcattaga aagaagattt ccatcagtag gtgacgttgc agatgcagct 780
gtatttttag cttcagctga atctgccgca ttgtctggtg aaaccatcga agtaacacat 840
ggtatggaat tgccagcttg ttctgaaaca tctttgttgg caagaacaga tttgagaact 900
atagatgcat ctggtagaac aaccttaata tgtgctggtg accaaattga agaagttatg 960
gctttgactg gtatgttaag aacttgtggt tctgaagtta ttataggttt cagatccgct 1020
gcagctttgg cacaattcga acaagccgtt aatgaatcta gaagattggc aggtgctgat 1080
tttacccctc caatcgcttt accattggac ccaagagatc ctgctacaat tgatgcagtt 1140
tttgattggg gtgcaggtga aaatactggt ggtatccacg cagctgttat cttgcctgca 1200
acctctcatg aaccagcccc ttgtgttata gaagttgatg acgaaagagt cttgaacttc 1260
ttggctgatg aaatcacagg tactatcgta atcgcttcta gattggcaag atactggcaa 1320
tctcaaagat tgacaccagg tgccagagca agaggtccaa gagtaatttt cttgtcaaac 1380
ggtgctgatc aaaatggtaa tgtatatggt agaatacaat cagctgcaat aggtcaattg 1440
atcagagttt ggagacacga agctgaattg gattatcaaa gagcttctgc tgcaggtgac 1500
catgtattgc ctccagtttg ggctaatcaa atcgtaagat tcgcaaatag atctttagaa 1560
ggtttggaat ttgcctgtgc ttggaccgct caattgttac attctcaaag acacatcaat 1620
gaaatcacct tgaatatccc tgctaacatc tcagcaacca caggtgcaag atccgcttca 1680
gttggttggg ccgaatcttt aataggtttg cacttgggta aagttgcatt gatcacaggt 1740
ggttctgctg gtatcggtgg tcaaatcggt agattattgg cattgtcagg tgcaagagta 1800
atgttagcag atgttgatac tagaaagtta gaacaaatgc aagccatgat acaaagtgag 1860
ttagccgaag taggttacac tgacgtagaa gatagagtcc atatagcacc aggttgtgat 1920
gtctcctccg aagcccaatt agccgatttg gttgaaagaa ctttgtccgc tttcggtaca 1980
gttgattatt tgattaataa cgccggtatc gctggtgtag aagaaatggt tatagatatg 2040
ccagtcgaag gttggagaca tacattattc gcaaatttga tctccaacta cagtttgatg 2100
agaaagttgg ctcctttgat gaaaaagcaa ggttctggtt acatcttgaa tgtttcttca 2160
tactttggtg gtgaaaaaga tgctgcaatt ccatatccta acagagcaga ctacgccgta 2220
tcaaaggcag gtcaaagagc tatggctgaa gtttttgcta gattcttagg tccagaaatc 2280
caaattaatg caattgcccc aggtcctgtt gaaggtgaca gattgagagg tactggtgaa 2340
agaggtggtt tgttcggtag aagagcaaga ttaatcttgg aaaataagag attaaacgaa 2400
ttgcatgccg ctttgatagc agccgctaga acagatgaaa gatctatgca cgaattagta 2460
gaattgttgt tgccaaatga cgttgcagcc ttggaacaaa acccagctgc acctactgct 2520
ttaagagaat tggcaagaag attcagatct gaaggtgacc cagctgcttc ttcttcttct 2580
gcattgttga atagatcaat tgcagccaag ttattggcta gattacataa cggtggttat 2640
gtattgccag ccgatatatt tgctaatttg ccaaacccac cagatccatt tttcacaaga 2700
gcacaaattg atagagaagc cagaaaagtt agagatggta taatgggcat gttgtacttg 2760
caaagaatgc caactgaatt tgatgttgct atggcaacag tctattactt agctgacaga 2820
aatgtttctg gtgaaacttt tcatccatca ggtggtttga gatatgaaag aactcctaca 2880
ggtggtgaat tattcggttt gccatcccct gaaagattag ctgaattggt cggtagtacc 2940
gtatacttaa ttggtgaaca tttgactgaa cacttgaatt tgttggccag agcttatttg 3000
gaaagatacg gtgcaagaca agttgtcatg attgttgaaa ccgaaactgg tgctgaaacc 3060
atgagaagat tattgcatga tcacgtcgaa gccggtagat tgatgactat tgtagctggt 3120
gaccaaatag aagctgcaat cgaccaagct attacaagat atggtagacc aggtcctgta 3180
gtttgtacac cttttagacc attacctacc gtccctttgg taggcagaaa agattctgac 3240
tggtcaaccg ttttatctga agccgaattt gctgaattat gcgaacatca attgactcat 3300
cacttcagag ttgcaagaaa gattgccttg tccgatggtg caagtttagc cttggtcacc 3360
ccagaaacta cagcaacttc caccactgaa caattcgcat tggccaactt catcaagaca 3420
accttgcacg ctttcacagc aaccatcggt gtcgaaagtg aaagaactgc tcaaagaatc 3480
ttaattaacc aagtagattt gacaagaaga gctagagcag aagaaccaag agatccacat 3540
gaaagacaac aagaattaga aagattcatt gaagctgttt tattggtcac agcaccattg 3600
ccacctgaag ccgataccag atacgctggt agaatacata gaggtagagc tataacagtt 3660
taa 3663
<210> 46
<211> 777
<212> DNA
<213> BDHcm
<400> 46
atggtcaact tacaaggtaa aacagcattg gtcactggtt caacatcagg tatcggtttg 60
ggtatcgcag aagcattggg tagagccggt gctaatatag tattaaacgg ttttggtgac 120
gttgatgctg cattggcaac aatcgccgct accggtgcac aatctgccca tcacccagct 180
gatatgagaa aacctgacga aattgaagca atgatagcta tggcaagaga aagattcggt 240
accgttgatg tcttagtaaa taacgctggt attcaacatg ttgcacaagt cgaagatttt 300
ccagcacaaa agtgggatga catcttggcc ataaatttga cttcttcatt ccatactaca 360
agacacgtta tacctgctat gagagcaaga aattggggta gaatcgttaa cattgcttca 420
gtacatggtt tagttggttc cgccggtaaa agtgcttatg ttgcagccaa gcacggttta 480
gtcggtttga ccaaagtaac tgcattggaa acagccggta ccggtatcac ttgtaatgct 540
atttgcccag gttttgtttt aacacctttg gtcgctgcac aaatagatgc tattgctaaa 600
agagatggta tttctatgga agccgctaga gctagattgt tatctgataa gcaaccatca 660
ggtcaatttg tcactcctga acaattgggt aacttggttt taatgttgtg tagtccattc 720
ggtgaccaag ttagaggtgc agcctgggct atggatggtg gttggacagc tcaatga 777
<210> 47
<211> 771
<212> DNA
<213> BDHkp
<400> 47
atgaatttgc atggtaaaac agcattagta actggttcta catcaggtat cggtttaggt 60
atcgccaagg tcttagcaca agcaggtgca caattggtat taaatggttt tggtgactct 120
tctcatgcta gagcagaagt tgctgcattg ggtaaaatac caggttatca cgatgctgac 180
ttaagagatg ttggtcaaat tgaagctatg atgcattacg cagaatccac cttcggtggt 240
gtagatatcg ttattaataa cgccggtatt caacacgtcg ctccagtaga acaatttcct 300
gtcgataagt ggaatgacat attggctatc aacttatcca gtgttttcca tactacaaga 360
ttggcattac ctggtatgag acaaagaaac tggggtagaa ttataaacat tgcatctgtt 420
cacggtttgg tcgcatctaa agaaaagtca gcctatgttg ccgctaaaca tgccgttgtc 480
ggtttgacca agactgtcgc attagaaaca gccagatcag gtatcacctg taatgctatt 540
tgcccaggtt gggtattgac tcctttagtt caacaacaaa ttgataagag aatagccgaa 600
ggtgttgacc cagaacaagc ctccgctcaa ttgttagctg aaaagcaacc aagtggtgaa 660
tttgttacac ctcaacaatt aggtgaaatg gcattgttct tatgttctga tgcagccgct 720
caagtcagag gtgcagcctg gaacatggac ggtggttggg tagctcaatg a 771
<210> 48
<211> 1113
<212> DNA
<213> HBDHos
<400> 48
atgcaattat tcaagttaaa gaccagatta tcaaagttcg atcatttcgc agatttcgcc 60
agagagttta agttatcaga acatgacttg gttatcacta acgaattttt gtacgaacct 120
ttcatgaagt cattaaactt gccatgtcat ttcattatgc aagaaaaatt tggtttaggt 180
gaaccatcag acaagatgat gaacacaatt ttgaaagaag ttaagggtgt tggtttcgat 240
agagtaatag ctgttggtgg tggtactgtt atcgacattt caaaattgtt cgttttaaag 300
ggtgttgaag atgtcacaga agcattcgaa agaaaaattc caatcgtaaa agaaaagcaa 360
ttagttatct tgcctacaac ttgtggtact ggttcagaag ttacaaatat ctcaatagca 420
gaaatcacat caaagcatac caagatgggt ttggcagacg atgccatcgt tgcagatgac 480
gctgtattgg ttccagaatt gttaaaaggt ttacctttta agttttatgc ctgttctgct 540
attgacgcat tggttcatgc taccgaatca tatgtttctc caaaatcaaa tccatacact 600
agattatatt gtagagctgc cattgaaata atcattgatg tttttcaagg tatcgctgca 660
aacggtccag aatacagatt tgaaagattg ggtgacatgt taatggctag taactacgct 720
ggtatagctt ttggtaacac tggtgttggt gcagttcatg ccttgtcata cccattaggt 780
ggttcctacc atgttccaca tggtgaatcc aactaccaat tctttacaga agtttttaag 840
ttatataatg ccaagaaccc atcaggtgac atagctagat tgaatgactt gttatgtgta 900
caattgggtg ttaatgaaaa cccttaccca gttttagacg aattgttggg taaattgatt 960
cctaaaaaca aattgagagt ctacggtatg aagccagaag aaattcaagg tttcacatcc 1020
tcagttttac aaacccaaca aagattgtta gcaaacaact atgtatcctt atccgaagaa 1080
gaaatcagag aaatctacaa agtattatat tga 1113
<210> 49
<211> 1137
<212> DNA
<213> HBDHps
<400> 49
atggccttca tctattacgt cacacaaatc caatttgaat ttggtgccgt caagttatta 60
aagcaagaat gcgaaagagt aggtatcact agaccattga ttgtcacaga tcctggtgta 120
aaagctgcag gtatattaca aaaggcttta gatgcattgc caggtatcgc tgtaactgtt 180
tttgaccaaa caccatcaaa tcctaccgaa gccgctgtta gagcagccgc tgcattgtat 240
aaagcaggtg gttgtgatgg tttaattgct gttggtggtg gttccgcaat agactgcgcc 300
aagggtgttg ctatcgccgc tacacatgaa ggtcctttga ctacatacgc taccatagaa 360
ggtggttcac caaaaatctc cgatagagtt gcaccattaa ttgccgtccc taccacttct 420
ggtacaggtt ctgaagtagc cagaggtgct ataatcatcg ttgatgacca tagaaagttg 480
ggttttcatt catggcactt agttccaaag accgcaattt gtgatcctga attgacttta 540
ggtttgccag ctatgttgac agcagccacc ggtatggacg ccattgctca ttgcatggaa 600
acctttatgt ctccagcttt caatccacct gcagatggta ttggtttaga cggtttggaa 660
agaggttggt cacacataga aagagctact agagatggtt ccgacagaga agcaagatta 720
aacttgatgt ctgcttcaat gcaaggtgca atggccttcc aaaaaggttt aggttgtgtc 780
cattccttaa gtcactcttt gggtggtgta gatcctagat tacatcacgg tacattgaat 840
gccatgtttt taccagctgt tgtcagattc aacgctagtg cagaatctgt tcaaaaggaa 900
agaagattgg atagaatggc cagagctatg ggtttaagat caggttccga tatccctgac 960
gcaattaagg atatgaatgc cagattaggt ttgccaactg gtttggctgc aatgggtgtt 1020
caaagagatt ggttcgacag aattatagtc ggtgcattgg ccgatcattg ccacaagact 1080
aacccaagat tagctagtcc tgaagaatat agagaaatgt tagaaacatc tttgtga 1137
<210> 50
<211> 867
<212> DNA
<213> HIBADHas
<400> 50
atgcaaacta tcggtttcat cggtttaggt gttatgggta aatctatggc aagacactta 60
ttgagagcag gttattcatt ggtcgtttat accagaacta agcaaaaggc agaagatata 120
ttggctgaag gtgcattgtg gaaagaaact gtaaaggatg ttgctaaagc tgcagacgtt 180
gtcatgacaa tggttggtga accacaagat gtcgaatatg tatactttgg tgacgatggt 240
atcttggctc atgcaaagag aggtacatac gtcattgatt tcactacatc caaaccaagt 300
ttagctgtaa gaatacacga agccgctaaa gaaaagggta tctttgtttt ggatgcacct 360
gtctctggtg gtgacattgg tgctagagat ggtaccttat caataatggt tggtggtgac 420
aaggaagcct tctctgcttg tatgccattg ttgtcacatt tgggtaaaaa tatagtctgg 480
caaggtgaag ccggtgctgg tcaacacaca aagatgtgta atcaaattgc aatagccacc 540
aacatgatag gtgtatgcga agcattagtt tatgcagaaa aagccggttt agatcctgaa 600
agagttttga gatccattag tcaaggtgca gcctcttcat ggtctttatc aaacttggct 660
ccaagaatgt tggctggtga ctttgcacct ggtttctacg ttaagcattt catcaaggac 720
atgggtattg ctttagcaga agccgaaacc ttgggtttat ccttgcctgg tttaaaattg 780
gccaagcaat tatacgaatc cttggctgaa agaggtgaag aacaaagtgg tactcaagct 840
ttgtacaagt ggtacaagga atggtga 867
<210> 51
<211> 891
<212> DNA
<213> HIBADHbc
<400> 51
atggaaatcg cattcatcgg tttgggtaac atgggtggtc ctatggctgc taacttatta 60
aaggctggtc acgcattgac tgtattcgac ttagatgcac atgctgttga tgctgcagtt 120
agagctggtg ctgcagcagc aacttcacca agagatgctg cagccagagg ttctttaatc 180
attacaatgt tgcctgccgc acaacatgtt agagccgttt atttgggtga cgatggtgtt 240
ttagccggtg caagagccgg tgcaaccttg atcgactgtt ccacaattga ccctggtaca 300
gtcagagctg ttgcagatgc cgctgcacaa agagacttcc ctttggctga tgcaccagtt 360
tctggtggta caggtggtgc tcaagcaggt actttgacat tcatggttgg tgctgatggt 420
gcattgtttg aaagaattag acctgtttta ttggacatgg gtagaaatgt agttcactgt 480
ggtggtactg gtacaggtca aatcgctaag atctgtaaca atttgttgtt aggtatttca 540
atgatgggtg tttccgaagc aatggctttg ggtgcagcct tgggtatcga cccaaccgtt 600
ttagcaggta taatcaacac ctctacaggt agatgttggt cctctgatgc atacaaccca 660
tatcctggtg tttcagacac agcaccagct gcaagaggtt atgcaggtgg ttttgcagcc 720
aacttgatgt taaaagactt gggtttagca actgaagctg caagatcagc ccatcaacca 780
gtttggatgg gtgctttagc acaacaattg taccaaagta tgtcccaaca aggtttaggt 840
acattagact tttccgcctg cgtcaaatta tacgaagccc aaccagcatg a 891
<210> 52
<211> 888
<212> DNA
<213> HIBADHma
<400> 52
atggctaaaa taacattcat tggtttgggt aacatgggtg gtcctatggc tggtaacttg 60
gtaaaagcag gtcacgaagt cacagtattt gatttgtcca aagacgctgt cgctgcatta 120
gtaagtgaag gtgcaaagac cgccggtact gctcatgaag ccgcttcagg tgcagaatgt 180
gttataacta tgttgccagc aggtcaacac gttgaagccg tctatttggg tgacgatggt 240
ttgttagcaa aattgccagc cggtacatta gttattgatt cttcaacaat agctcctgaa 300
accgcaagag gtgtagctga agttgcaaga gccaaagata ttccattttt ggacgctcct 360
gtttctggtg gtgtcggtgg tgctaaggca ggtactttaa cttttatttg tggtggttca 420
gaagaagctt ttaataaggc aaagcctatc ttggatacta tgggtaaaaa tatcttccat 480
gctggtgacc acggttctgg tcaagttgca aagatatgca ataacatgtt gttagccatc 540
ttaatgtccg gtacaagtga agccttggct ttaggtgtca aaaatggttt agatccagct 600
gttttatccg aaattatgaa gcaatccagt ggtggtaatt gggcattgaa tgtttataac 660
ccttggcctg gtgtaatgga aggtgttcct gcttctagaa attacgaagg tggtttcttg 720
gttaacttaa tgactaagga tttgggttta gccttcgaca atgctgtcaa gaaccaagct 780
tctatcccaa tgggttcatt agcaagaaat ttgttccaat tgcatgccgg tcagggtaac 840
ggttctttgg atttctcttc aattcaaaga ttatacaaac ctgaatga 888
<210> 53
<211> 897
<212> DNA
<213> HIBADHpa
<400> 53
atgacagata ttgctttctt gggtttgggt aacatgggtg gtcctatggc tgctaactta 60
ttgaaggctg gtcacagagt aaatgtattt gatttgcaac caaaagctgt attgggttta 120
gttgaacaag gtgcacaagg tgccgactct gctttacaat gttgcgaagg tgccgaagtt 180
gtcatttcta tgttgcctgc aggtcaacat gtcgaatcat tgtatttggg tgacgatggt 240
ttgttagcaa gagtagccgg taaaccattg ttgattgatt gttcaactat agctcctgaa 300
acagcaagaa aagttgccga agctgcagcc gctaagggtt tgaccttgtt agacgctcca 360
gtctccggtg gtgtaggtgg tgcaagagcc ggtactttaa gttttatagt aggtggtcca 420
gctgaaggtt tcgctagagc aagacctgtt ttggaaaata tgggtagaaa catctttcat 480
gcaggagatc atggtgctgg tcaagttgca aagatctgca acaacatgtt gttgggtatt 540
ttaatggctg gtacagccga agctttggca ttaggtgtta aaaatggttt agacccagct 600
gttttgtccg aagtcatgaa gcaatcttca ggtggtaact gggcattgaa tttgtataac 660
ccttggcctg gtgttatgcc acaagcccct gcttctaatg gttacgctgg tggtttccaa 720
gtcagattga tgaataagga tttgggttta gcattggcca acgctcaagc agttcaagct 780
tctacccctt taggtgcctt ggctagaaat ttgttttctt tgcatgcaca agccgatgct 840
gaacacgaag gtttagactt ctccagtata caaaagttgt atagaggtaa agattaa 897
<210> 54
<211> 888
<212> DNA
<213> HIBADHxc
<400> 54
atgtctaaaa tcgccttcat cggtttgggt aatatgggtg gtcctatggt cgctaacttg 60
ataaaagcag gtcacgaatt gagagtattt gatttggttc attctgcttt agacactgca 120
cacgctgcag gtgctcaagt cgcatcttca gcctcagata cattacatgg tgcagaagtt 180
gtcatttcta tgttgccagc ttcaaaacac gttgaaggtt tgtatttggg tgacgctggt 240
attttagctc atataccagc aggtgccttg gttatcgact gttccaccat tgctcctgca 300
atggccagaa ccgttgcaac tgccgctcac gccagaggtt tggatatgtt agacgcacca 360
gtttctggtg gtacagctgg tgcagccgct ggtactttaa cttttattgt aggtggtgca 420
gcccaagcat tggaaagagc cagacctgtt ttacaagcaa tgggtaaaaa cttgttccat 480
gttggtgaca atggtgctgg tcaagtcgca aagttgtgta ataacatggc tttaggtgtc 540
ataatggctg caactggtga agctatcgca ttaggtgtag cccaaggttt ggacccagct 600
gtattatcac aaatgatggc agtttctaca ggtagatcat gggctaccga agtctgcaac 660
ccttggcctg gtgtattgga aaatgcccct gcttccagag gttatagtgg tggttttggt 720
aacgatttga tgttaaagga cttgggttta gttgctgaag ccgctgtcca agcaggtgcc 780
tccattcctt tgggtgaatt agctagaaat ttgtacgcaa tgaacagtag agccggtaat 840
ggtgctttag atttctccag tgtagttaaa ttggtcgcta aggtatga 888
<210> 55
<211> 762
<212> DNA
<213> HPDHam
<400> 55
atgacacaac aaacagtatt cgttacaggt gcctccgcag gtttcggtgc cgccatagca 60
agaagattcg tagcagaagg tgccagagtt attgccgtcg ctagatctga agataaattg 120
gaaaagttag ccggtgaatt gggtgacgct gtattgccag ttaaattaga tgtctcagac 180
cctgaagcag taaaggaagt tgtcacaaat ttgccagccg attggcaaca agttgatgta 240
ttagttaaca acgctggttt ggcaaaaggt ttagaacctg cccataaggc tgatttgcaa 300
gattgggacg aaatgatagc taccaatgtt agaggtttaa cccatgtcac tagagcattg 360
ttaccaggta tggttgaaag aggtagaggt cacgttatta atataggttc tatcgctggt 420
acttatccat accctggtgg taacgtttat ggtgcaacaa aagcctttgt tcatcaattc 480
tccttgaatt tgagaagtga tttgcacggt acaggtgtca gagtaaccaa cgtagaacct 540
ggtatggttg gtggtaccga tttttccaag gttagattcg atggtgacca agcaaaagcc 600
gataaggttt acgaaggtac tacaccatta actgctgaag atgtcgcaga aagtgtattt 660
tgggctgcaa atcaacctgc acacgttaat atcaacgtca ttgaattgat gccagtagtt 720
caatcttttt cacctttgca aatattcaga gataactcat ga 762
<210> 56
<211> 1653
<212> DNA
<213> HPDHbs
<400> 56
atgaccgctg gtaaaggtaa aggtgctaaa gttgaccctg ctggtggtaa agccgctact 60
ggtagattgg ccggtaaagt tgcattggtc acaggtgcag ctggtaattt gggtggtgcc 120
attattagaa gattcattgc cgaaggtgca actttggtta tgactggtag aactaaggaa 180
agattagctg cagcaaagca agctttattg gacgaaactg gtgttgaaga agcaagagtt 240
tttaccgtaa tcttggatgg tgctgatcca gattcagtta gagcaggtat tgatcaagtc 300
agagctgcct taggtagaat tgatgttttg gtcaataatg caggttcagc tggtcctaga 360
caaacattgg aaaacgtacc attgactaga gccgaaatgg aagcaaatgg tgactcagaa 420
acagtagctg acgccatgag aaatttgttg ggtgtcagtt ggaacttaac aagagcagct 480
gccccaataa tgccagtagg tgcatcagtc gttaatattt ccaccatctt ctctcacaca 540
agatactatg gtagaacagc ctatgtcgtt cctaaggctg cattaaacgc cttatcccaa 600
gaattagcaa aggaattagg tgcatctggt ataagagtaa atacagtttt cccaggtcca 660
atcgaaagtg aaagaattag aacagttttc gcagctatgg atgccttgca aggtgaacca 720
gaaaactcta ctgcaaatca tttcatgggt agaatgacat tggaaagagc tatcggtgac 780
gcccctaaaa gaaagacttt gccagttcca ggtgacattg cagatgcttg tttgttttta 840
gcatcagatg aaagtgctgc cttaaatggt aacgaaatag atgttacaca tggtatgcaa 900
gttagaaaag atagtagatc aacttacatg actagaccat ccatgagatc tttggacggt 960
gcaggtttga ccgtcttaat tgccgctggt gaagcatggg aagatgcatt agatatggct 1020
aagatccaag ctgcatgtgg tgccagagtc ttattgggta tgactagaca agttgacgtc 1080
gcacaagccg aagccagagt taaagctcaa ggtgctgaag aaggtttaac catcatgaga 1140
ttcaacagag ctgaacctga tggtatggaa gcagccataa gagcctattc tgatgcacat 1200
ggtgcaatcc actctgccat cgttttacct gtaaagccag ctggtcattt cggtgactct 1260
ttggttgaag cttcagatgc agaagttgac gctttgattg atatcgaatt aaccggtgct 1320
attgcagttg ccagaacctt gagaagatac tggaaaacat gctctgactt gatgcaagat 1380
ccaagattcg tctttatgtc aggtccatct gatggtaaag gtaacaaagc aggtagaata 1440
ttagcagccg caatagccca attaatcaca atttggagag atgaatctag agtagacttt 1500
gcacaaggta gacaaaagag agccgcatgg ggtaatcaaa tagttagata cacaaatgaa 1560
gaagatgaaa acaccagatt cgccgcaggt catgctacta gaattgtttt caaggaacaa 1620
aagataactg aaacttcctt gtacttgcct taa 1653
<210> 57
<211> 1653
<212> DNA
<213> HPDHca
<400> 57
atgagtggta caggtagatt ggctggtaaa atagcattga taacaggtgg tgcaggtaat 60
ataggttccg aattgacaag aagatttttg gctgaaggtg caactgttat catatcaggt 120
agaaatagag ctaaattaac tgctttggcc gaaagaatgc aagcagaagc aggtgttcca 180
gctaaaagaa tcgacttaga agtcatggac ggttccgatc ctgtagctgt tagagctggt 240
atagaagcta ttgttgcaag acacggtcaa atcgacatct tggtaaataa tgctggttct 300
gcaggtgctc aaagaagatt ggctgaaatc cctttgactg aagcagaatt gggtccaggt 360
gctgaagaaa ccttgcatgc ttctattgca aacttattgg gtatgggttg gcatttgatg 420
agaatagctg cacctcatat gcctgtaggt tctgccgtaa ttaacgtttc caccatattc 480
tcaagagcag aatactatgg tagaatccca tatgttaccc caaaagcagc cttgaatgca 540
ttgtcacaat tggctgccag agaattgggt gccagaggta tcagagttaa taccatcttt 600
ccaggtccta ttgaatccga tagaatcaga accgtatttc aaagaatgga ccaattgaag 660
ggtagacctg aaggtgacac agcacatcat ttcttgaata ccatgagatt gtgtagagcc 720
aatgaccaag gtgcattaga aagaagattt ccatcagtag gtgacgttgc agatgcagct 780
gtatttttag cttcagctga atctgccgca ttgtctggtg aaaccatcga agtaacacat 840
ggtatggaat tgccagcttg ttctgaaaca tctttgttgg caagaacaga tttgagaact 900
atagatgcat ctggtagaac aaccttaata tgtgctggtg accaaattga agaagttatg 960
gctttgactg gtatgttaag aacttgtggt tctgaagtta ttataggttt cagatccgct 1020
gcagctttgg cacaattcga acaagccgtt aatgaatcta gaagattggc aggtgctgat 1080
tttacccctc caatcgcttt accattggac ccaagagatc ctgctacaat tgatgcagtt 1140
tttgattggg gtgcaggtga aaatactggt ggtatccacg cagctgttat cttgcctgca 1200
acctctcatg aaccagcccc ttgtgttata gaagttgatg acgaaagagt cttgaacttc 1260
ttggctgatg aaatcacagg tactatcgta atcgcttcta gattggcaag atactggcaa 1320
tctcaaagat tgacaccagg tgccagagca agaggtccaa gagtaatttt cttgtcaaac 1380
ggtgctgatc aaaatggtaa tgtatatggt agaatacaat cagctgcaat aggtcaattg 1440
atcagagttt ggagacacga agctgaattg gattatcaaa gagcttctgc tgcaggtgac 1500
catgtattgc ctccagtttg ggctaatcaa atcgtaagat tcgcaaatag atctttagaa 1560
ggtttggaat ttgcctgtgc ttggaccgct caattgttac attctcaaag acacatcaat 1620
gaaatcacct tgaatatccc tgctaacatc taa 1653
<210> 58
<211> 1653
<212> DNA
<213> HPDHcag
<400> 58
atgtccgtca caggtagatt ggcaggtaaa atagccttga taacaggtgg tgctggtaac 60
ataggttcag aaatgactag aagattctta gccgaaggtg caactgttat tatctcaggt 120
agaaactcag ctaaattggc cgctttagca gaaagattga gatccgaagc aggtgttcct 180
gctaagagaa tagatttgga agttatggac ggttcagatc cagctgctgt tagagcaggt 240
gttgcagcta tcattggtag acatggtcac atagatatct tagttaataa tgcaggttcc 300
accggtgcac aaagaagatt ggctgaaatc ccattaaatg aaaccgatag agacttagac 360
gatgaagaag ctttgtctac aagtgttgca aatttgttgg gtatggcttg gcacttaatg 420
agaatcttaa gtcctcatat gccaccaggt tctgctatta taaacatctc caccatcttc 480
agtagagctg aatactatgg tagaatccct tacgtcgttc caaaggcagc cttgaataca 540
ttgactcaaa tcgctgcaag agaattgggt atcagaggta tcagagttaa cacaatcttt 600
ccaggtccaa ttgaatccga aagaatccaa acagtttttc aaagaatgga ccaattgaaa 660
ggtagaccag aaggtgacac agcatcacaa ttcttggcaa ctatgagatt atacagagct 720
aacgatcaag gtcaattgga aagaagattc ccaacaatct gtgacgttgc tgatgctgca 780
gttttcttgg cctctgatga agcagccgct ttgacaggtg aaactatcga agttacacat 840
ggtatggaat tgccaacatc ttcagaaacc tctttattgg ctagaacaga cttaagaact 900
attgacgcaa atggtagaac cactttgata tgtgccggtg accaaatcga agaagttatg 960
gctttgactg gtatgttgag aacttgtggt tctgaagtca ttatcggttt tagaagtgaa 1020
gctgcattgg cacaattcga acaagccatc ggtgaatcta gaagattggc tggtgaatcc 1080
tttatcccac ctattgcatt accaattgac ttgagaaacc caagtacaat agacgcatta 1140
tttgattggg ctggtgaaaa tactggtggt attcatgcag ccgtaatctt gccagcatca 1200
ggtagagaac ctgcaacaca agttatagac atcgacgatg ctcatgtcca agccttctta 1260
aacgatgaaa tcgtaggttc catcattatc gcatcaagat tggctagata ctggcaagca 1320
caaagaatcg cacctggtgc cagagcaaga gaacctagag tcatcttctt gtctaatggt 1380
gcatcaactg ctggtaatcc ttacggtaga atccaatccg cagctataga acaattaata 1440
agagtttgga gacatgaagc agccttggat tacgaaagag caaccgctgc aggtgaaaga 1500
gttttgcctg cagtttgggc atcacaaatc gttagatttg caaacagatc cttagaaggt 1560
ttggaatttg cctgtgcatg gaccgcacaa ttattacatt cccaaagaag aattaatgaa 1620
ataacattaa ctatccctgc cgacatctct taa 1653
<210> 59
<211> 1653
<212> DNA
<213> HPDHct
<400> 59
atgagaagaa caaaaccaga atgcttgaga agaaatgaaa gtacaatgag attgaacggt 60
aaagtcgcct taataacagg tgccgcaggt ggtatcggtt cagtagtcac aagaatgtac 120
ttgagagaag gtgctagagt tgcaatttcc tcaagatcct tggaaagagc agaacaattc 180
agagcagcct taatggccga aggtttttca ggtgacgata ttttgccagt agcatttgcc 240
agtacagact tagccggtat gcaaacagct atcgaagcaa ttgcagctag atggggtggt 300
ttggatgttt taattaacaa tgctggttca gctggtgcaa agcaaccttt gttcagaatc 360
cctttcacct cagaagattt ggctcaattg gcagctgaag gtttcacaga aaccgaaaca 420
atgagagaaa gtgctgccaa cttattgggt atgccttggc acttaacaag attggcttta 480
ccttacttga gagttggtgc ttctattatc aatgtatcta caatcttctc aagaaccaat 540
tattatggta gaataccata cgtagtccca aaatctgcct tgaacgcttt atccttgggt 600
ttggccaaac aattgggtac cactgacaga gctgtcagag ttaataccgt ttttccaggt 660
ccaatagatt cagacagaat cagaaccgtt ttcggtgcta tggacagatt gaaaggtgtc 720
ccagaaggta ctaccgctca agaatttttc gacattatga ttttggctag agcaagaggt 780
ggtgaagcac ctacaaagtg ttatccaact caagctgatg ttgctcacgt aattttattt 840
ttgggttcag atgaatctgc agccatatct ggtcataact ttgaaataac tcatggtatg 900
caagttaagg cacaatccag aacaaaattg actgcatggc cagaccaaag attgatagat 960
ttgtcaagaa gagttgtctt gattgttggt ggtgaacaag tacatgatgc tgtcacaata 1020
gcagcctccc aacaatcata tggtgcttca gttatcttga cattcagaca accagaagca 1080
gttaatactg caaaagctca attggaatcc agaggtttga accattctat agcagttacc 1140
tggttagacc cattgagaag agaatctgtt acaagtgttt tcgacttaat cagagaaaga 1200
taccacagat tggataccgt tatagttttt ccagctcatg ccgctggtca ttatggtcct 1260
gaattgatcc cagatgaacc tgaaagaatc agacaattca ccgatgaagc tattttaggt 1320
acaaccgcat tcgcagctca attgtccaga tttttattgg ctttcgatgc agaaaacgaa 1380
ttgcaagaac ctgtcaacgt tttgtttgtc acaaacgaac acgacggtgc tacaaatgca 1440
tttgaagata tctatagagc cgcagtcgaa caattgttga gagtttggag acacgaagat 1500
gaattgcaag ttgcagctgg tacaagaaga ttcgccgtaa gaaccaacca aatagtaaga 1560
tatgctaatg cagaaccaga caacttgaag tttacagccg attggttggc aacattagcc 1620
aacagagtca gaatctttga tgaattaaac taa 1653
<210> 60
<211> 747
<212> DNA
<213> HPDHec
<400> 60
atgatcgtat tagttactgg tgccacagcc ggtttcggtg aatgtattac aagaagattc 60
atccaacaag gtcacaaggt tatcgccacc ggtaggagac aagaaagatt gcaagaatta 120
aaggatgaat tgggtgacaa tttgtatata gctcaattgg atgttagaaa cagagctgca 180
atcgaagaaa tgttggcctc tttaccagct gaatggtgta acattgatat tttagttaac 240
aacgccggtt tggctttggg tatggaacct gcacataaag cctcagttga agattgggaa 300
actatgattg acacaaataa caagggtttg gtttacatga caagagcagt cttaccaggt 360
atggtagaaa gaaatcatgg tcacattata aacataggtt ctacagcagg ttcatggcct 420
tatgccggtg gtaatgtata cggtgctacc aaagcatttg ttagacaatt ctccttgaac 480
ttaagaaccg atttgcacgg tactgccgtt agagtcacag acattgaacc aggtttagtc 540
ggtggtactg aattttcaaa cgttagattc aagggtgacg atggtaaagc tgaaaagacc 600
tatcaaaaca ctgttgcatt gacacctgaa gatgtctccg aagccgtatg gtgggttagt 660
acattgccag ctcatgttaa catcaacacc ttagaaatga tgcctgttac tcaatcttac 720
gcaggtttaa atgtccacag acaatga 747
<210> 61
<211> 591
<212> DNA
<213> HPDHed
<400> 61
atgtcagaag caatcactcc tggtgcctta gaaactttat tcactggtgc aagaactcac 60
aacggttggc aaaatatgcc tgtatccgat gaaactttga gagaaatcta taatttgatg 120
aaatggggtc caacatctgc aaactgttca cctgccagaa ttgttttcgt cagatctgcc 180
gaaggtaaag aaaagttaag accagcatta tcttcaggca atttggaaaa gactttaact 240
gctcctgtaa ctgcaatagt tgcctgggat ggtgaatttt acgaaagatt gccagaatta 300
ttccctcatg gtgacgctaa gtcctggttt acagcaagtc cagccttggc tgaagaaacc 360
gctttcagaa actccagttt gcaagctgca tacttaattt ttgcttgtag agcattgggt 420
ttagataccg gtcctatgtc tggtttcgat agacaaaagg ttgatgaagc atttttcaca 480
ggtactacat tgaagtcaaa tttgttaatt aacataggtt atggtgaccc aggtaaattg 540
tacggtagat tgcctagatt agactttgat gacgcctgcg gtttagcttg a 591
<210> 62
<211> 1653
<212> DNA
<213> HPDHgb
<400> 62
atgaacgata tgtccgatat tccacaagac ttattaacac aaagtatagc aggtagaaga 60
ttagataaca aatgtattat tttaactggt gcagctggtt caatcggttc ctttattaca 120
agacaattat tgagagaagg tgcaagagtt atgttaacag gtagagatca atcaaaattg 180
gacgacttta ttggtgactt ggtatcagaa ggtttcgaag aaactttcat ggtttccgca 240
actggtgact gtgccgatcc tgaagtctgt agagaaatag ttgccaagac agttgccgca 300
ttcggtccta ttgatgtttt agtcaacaat gccggtgccg caggtccaaa attcacattg 360
agagatatcc ctttcaccga agcagaacaa agagcagctg actcagatca aacaatgttt 420
gactcagcca tgaatttgtt gggtgctcct tggaatatgg ccagagccgc tgcaccacat 480
atgtcagctg gtgcctctat aatcaacgtt agtactatct tttctagaac tcattactat 540
ggtagaatac cttacgtcgt tccaaagtct ggtttgaacg cattgggtaa aggtttggca 600
ttagaattgg gtgaaaaaca aggtatcaga gtaaacactt tatttccagg tcctattgaa 660
tccgaaagaa ttgaatcagt tttcgccaga atggatgaat tgcaaggtgt tgaacctggt 720
tcaactggta aagagtttag agatttgatg ataactacaa gaacctctga tggtggtttg 780
gaatataaat acccaactcc aacagatgtt gcatcaggta tcgtttggtt ggcatctgat 840
gaatcctcag ccttatctgg tcatcacatt gaagtcacca atggtatgca agtaccagca 900
caatctagat cccaattggt ttcatggcca gataagagat tggaagattt gtcaggtcaa 960
gtcgttttga ttttaggtgg tgacgactat caagaagcag ttactttcgc cgaaagacaa 1020
atccaatccg gtgcaagagt aatgttagct ttcagaaact tgaataccgt tggtcatgct 1080
agatcattag ttcaatcaag aaatttgggt gacgttcaat tatcccactt ggaccctttg 1140
agaagagaat cagttgacag atccatgcaa ttaatcgcag atcactttgg tagattagat 1200
ggtgtaatct tgttaccaca aaagccaaac ggtgaatacg gttattcctt gactacagct 1260
aatgacgaag atgttgccaa ttttgttaga gatgaagttg tcgcccctgt tgcatttgca 1320
tccgctttcg caagaaacat ggatcaattg tttgccgaag gtgaaccacc agccatcgtt 1380
tacgttacta accctagtga tagacatggt aacttgatga atgaaattat cagagcaagt 1440
gttgaagcct taatcagagg ttggagacat gaagatgaaa ctttggcaaa cactggtgaa 1500
ttgccttggg cagtacaacc aaaccaaatg gttagatatg ataccgaaga tagagaagct 1560
ttaactttcg cagccgactg ggcagccact ttaaccaaca gagttagaaa gatggatagt 1620
atcaacttat ggttaccaag aagtattaaa taa 1653
<210> 63
<211> 759
<212> DNA
<213> HPDHhw
<400> 63
atgatagtat tagtaacagg tgcctcctca ggtttcggta gagctattgc caagaagttc 60
ggtgaaaatg gtcataaagt aatcgcattg gcaagaagaa aggaaagatt gcaatcttta 120
caaaaggaaa tcccatactg tgaaatcttg ccttgtgata tctgcgacaa aaaggctgtc 180
aaacaagcat tggaagcctt accacaagat ttcaagtcta tcgacatttt ggttaacaac 240
gctggtttgg cattaggtat tgaacctgcc caaatgtgcg atttcaaaga ctgggaaaga 300
atgatatcag ttaatgtcac tgctttgtcc tatttgacac atttggtttt accacaaatg 360
gtcgaaagaa aaagtggtca cattataaca ttgggttcta ttgcaggtaa atatccatac 420
cctggtggta atgtatacgg tgccaccaaa gcttttgtta agcaattcgc cttgggttta 480
agagcagatt tggccggtac taacgttaga gtttcagaca ttcaaccagg tttggctgaa 540
agtgaatttt ctttagttag attcaaaggt gacaaaaaga gagctaatgc attgtatgaa 600
ggttccaacg ctttacaacc tcaagatatt gccgaagctg tctactgggt agcaacattg 660
ccaaagcata tcaacatcaa caccttagaa atgatgccta cttctcaatc attttccaca 720
ttggcagttc acaaagaata ctcattctta accaagtga 759
<210> 64
<211> 804
<212> DNA
<213> HPDHka
<400> 64
atgtctcaag gtagaagagc agccgataga ttagccggta aagtcgtatt gataactggt 60
gcctccgcag gtattggtga agccactgca tgggaatatt tgtttgcatc caatggtcaa 120
gccaaattga tattgacagc cagaagattg gaaaagttgg ctgctattaa agaagaaatg 180
aaaaaggaat acccaaacgt taagatacat gtcgctaagt tggatgtttc taacacagaa 240
gaaatcaaat cattgttaca atgtttacct accgagttta aatctattga tatattggtc 300
aacaacgccg gtaaagcttt aggttcagaa aaagtaggtg acattgctat tgaagatata 360
aagggtatga tggatactaa cgttataggt ttgattaatg taacacaagc agttttgcca 420
atcttgaagg aaaagaactc tggtgacatc gtcaacattg gttcagtagc cggtagagat 480
gcttatccta ccggttctat atattgcgct agtaaatttg cagttagagc cttcaccgaa 540
tctttgagaa aggaattgtt ggatactggt attagagtta tattgatcgc accaggttta 600
gtagaaactg aattttcctt agttagatac aagggtgacg aaaagagagc caatagtgtt 660
tacgaaggta tcactgcttt ggaagcagaa gatgttgctg aattaatcgt tttctctaca 720
tcaagaaaac caaacactgt tatagctgat acattgatct ttcctacctg tcaaggttcc 780
gcattccatg tccacagaag ttga 804
<210> 65
<211> 945
<212> DNA
<213> HPDHms
<400> 65
atgacagaaa aagtatcagt agtaggtgct ggtgttattg gtgttggttg ggccacatta 60
ttcgcctcta aaggttattc agtatctttg tacacagaaa agaaagaaac cttggataag 120
ggtatagaaa agttgagaaa ctacgtacaa gttatgaaga acaactccca aatcactgaa 180
gatgtcaaca cagtaatctc cagagttagt ccaactacaa atttggacga agctgttaga 240
ggtgcaaact ttgttattga agccgtcata gaagattacg acgctaaaaa gaaaattttc 300
ggttacttgg attctgtctt ggacaaggaa gttatattgg cctcttcaac ctctggtttg 360
ttaatcactg aagttcaaaa ggcaatgtca aagcatccag aaagagcagt tattgcccat 420
ccttggaatc cacctcactt gttaccattg gttgaaatag tccctggtga aaagacttct 480
atggaagttg tcgaaagaac caagtcattg atggaaaagt tggatagaat cgtagttgtc 540
ttaaagaaag aaatccctgg ttttattggt aacagattgg cctttgcttt attcagagaa 600
gctgtatatt tggttgacga aggtgtcgct actgtagaag atattgacaa agttatgaca 660
gctgcaatag gtttaagatg ggcttttatg ggtccattct tgacctacca tttgggtggt 720
ggtgaaggtg gtttggaata tttctttaat agaggttttg gttacggtgc aaacgaatgg 780
atgcacacat tagccaagta tgataaattc ccttacaccg gtgttactaa ggctatccaa 840
caaatgaagg aatactcctt tatcaagggt aaaactttcc aagaaatcag taagtggaga 900
gatgaaaagt tattgaaggt atacaagtta gtttgggaaa agtaa 945
<210> 66
<211> 1653
<212> DNA
<213> HPDHot
<400> 66
atgtttatga caagattgaa tgacaagatt gctttgatta ctggtggtgc tggtactata 60
ggtgaagtta ttacaagaag atatttggaa gaaggtgcaa cagtagttat ggctggtaga 120
aatagagaca aattagacag atatagagaa agattgataa ctgaatttca tgctttacca 180
gaaagagtta tggtagttag aatggacggt tcatccaacg cagaagttag aatgggtatt 240
gccgctgttg tagcccactt tggtagaatt gatatattgg taaataacgc aggttcagct 300
ggtgccagac aaagattgcc agctatccca ttattgagat cagaattgca agcagacgaa 360
accgaaactt tggcagattc aatcggtaac ttaataggta tcacatggaa tttgattaga 420
gctgcagctc cattcatgcc tgcaggttcc tcagttatca atattagtac aatctttgct 480
agaactgact actatggtag aatcccttat gttgtaccaa aggccgcatt acacgcctta 540
acattggcag ccgcaactga attaggtgaa agaggtataa gagttaacca aatcaaccca 600
ggtcctatcg actcagatag aattagaact gtttttagaa gaatggatga attgaagggt 660
gttccagaac aatcaactgc tgatggtttc tttcaaatga tgagattgag aaggcctaat 720
gccgaaggtg acttagttaa gggttttcct aagactttgg atgttgccaa cgttgctgta 780
tttttaggtt ccgcagaatc agctgcattg tccggtgaaa cattggatgt tacacacggt 840
atggcagttc caacagaatc aagaacaact ttgacaagta gacctggttt gagagcagta 900
gacggtagtg gtcacacaac attaatttgt gttggtgacc aaatcgaaga agcagcagcc 960
ttaactggtg tattgagagc atgtggtgcc gaagtagtta tcggttttag atctagagcc 1020
gcaatcgcta gattcgacca cttgatcgaa agaggtagac acttaccatc tcaagaacat 1080
gttgcccctg ttttgttata tttgaatcct acagaaccag aatcaataga ccaagccttg 1140
agatggatgg ccactaactt agacttgcct acaagtgtaa tcattttgcc tgcacaaaga 1200
caaccattac ctcctagtgt tgttagagcc tcagatgaag aagttgcata ctttttaaga 1260
gatgaattga gtggtatgat cgtcttggca agtagattag caagattctg gcaacaagca 1320
acattggcac ctggtaatgc acctattcaa ccaagagttt tattcatgac aaacccagac 1380
gatggtcaag gtaacttata tgcagaaata ttgagagcag gtgttgaaca attgtgtaga 1440
gtttggagac acgaatccca attggattat actagattgg cccaaatgga tgcacatcca 1500
cctcatatta gaccagtttg ggcaaaccaa ttggttagat ttgccaacaa tgaacaagaa 1560
aatttggaat actgctgtgc ttgggttgcc aagatattgt tatccgaaag aacaattgaa 1620
gaattaaact tatacttgcc tagacaaata taa 1653
<210> 67
<211> 816
<212> DNA
<213> HPDHps
<400> 67
atgtcctatg gtagtaaggc cgcagaaaga ttggctaaca aggtcatttt aataacaggt 60
gcttccgccg ccgctggtat cggtgccgca accgccagag aatttgctga agctgcacat 120
ggtaacataa agttgatctt gacagcaaga agaaaggata agttggacgc tttagcaaaa 180
gaattgaccg ataagtatga caacattaaa gtccacaccg tagccttgga tgttactaag 240
ttagaatcta ttggtcaatt cgtcaaggat ttgcctcaag aatttgcaga tatagacgtt 300
ttagtcaaca acgctggttt ggcattaggt agagatccaa tcggtgaaat tttgcctgaa 360
gatatacaag gcatgttcca aacaaacgta ttgggtttaa tcactttgac acaagcagtt 420
ttgccaatct tcaaagctaa aaattctggt gacatcgtta acttaggttc aattgctggt 480
agagatccat atcctggtgg tggtatctat tgtccaacta aagccgctgt aaagtctttc 540
tcacatgttt tgagaaagga attgattaat acaagaatca gagttttgga agtcgatcct 600
ggtaacgttg aaaccgaatt ttccatcact agattcaaaa acgatgttga taaggccaag 660
gctgtatatg ctaacactga accattagtc gccgaagata ttgctgaagt aatagttttt 720
ggtatcacaa gaaagcaaaa caccgttatt gcagaaactt tgatattctc aacaaaccaa 780
gcatccgcca gtcacttata caaaggtcaa gtttga 816
<210> 68
<211> 750
<212> DNA
<213> HPDHra
<400> 68
atgataatat tcgtcacagg tgccacagca ggttttggtg cagaaatcgc aagaaagttc 60
gtaaaggaag gtcacaaggt aatcgcaaca ggtagaagaa aggaaagatt agatgacttg 120
aaggctgaat taggtgacgc tttgtatgcc gtcgaattag acgtaaccaa tagagcatct 180
atagaagaag ctattgcttc tttgccagct gattggcaaa acattgacgt tttggtcaat 240
aacgctggtt tggcattagg tttggaaaaa gccgataagg cttccttgga agattgggac 300
aagatgatag atactaacac aaagggttta gtttacatga ccagagcagt attgccaggt 360
atggttgaaa gaaataccgg tcatgttatt aacataggtt ctactgccgg taattggcct 420
tatgctggtg gtaacgtcta cggtgcaact aaggccttcg taagacaatt ctcattgaat 480
ttgagaaccg atttgcatgg tactcacgtt agagttacaa atattgaacc aggtttgtgt 540
ggtggtacag aattttccaa cgttagattc aagggtgacg atgaaaaagt cagtaagacc 600
tacgataaca ctaacccttt aacagctgca gacgttgctg aatccgtctt ttgggctagt 660
acattgccag cacatgttaa catcaactct atcgaaatga tgcctgtttc tcaatcatta 720
gcaggtttgc aagttcacag agaaggttga 750
<210> 69
<211> 1653
<212> DNA
<213> HPDHrc
<400> 69
atgtctacag tcagaagatt ggaaggtaaa gttgcattga taacaggtgg tgccggtaat 60
ataggtgaag taataacaag aagatttttg gcagaaggtg ctaccgttgt tatcacaggt 120
agaaatgccg aaaaattggc tgtctacaga agaagattaa ttgacgaaga aagagtagca 180
ccagaaagag tcgttgcttt gagaatggat ggttctgaca ttgcccaagt tagagctggt 240
gttgctcaaa ttgttcacgg tggtacagat gtcccaatcc cattgcatag aatagatatc 300
ttggtcaaca atgcaggttc tgcaggtcca agaagaagat tagttgatat ccctttggaa 360
ccatccgaag ttcaacctcc agattcagaa actttggcac aagctgttgg taatttggtt 420
ggtataacat ggaatttgac aagagcagcc gcaccacaca tgccatccgg ttcatctgtt 480
attaacatct ctacaatctt ctcaagaacc gattactacg gtagaatagc ctatgtagca 540
cctaaggctg ctttgaacgc attatctgac ggtttggcca gagaattggg tgttagaggt 600
atcagagtca ataccatata tcctggtcca atcgaatcag aaagaatcta cactatgttt 660
caagctatgg acgctttaaa gggtcaacca gaaggtgaca cagctagtgg tttcttaaga 720
atgatgagat tgtctagaat cgaccaaaac ggtgaagttg ttaagagatt cccaagtcct 780
gtagatgttg caaatactgc tgtattcttg gcatctgacg aaagtgcagc ttttacaggt 840
catgcctttg aagtcacaca cggtatggaa gttccaaccg aaagtagaac cactttcgtt 900
tccagaccag gtttaagatc cgttgatgca accggtaaag ttattttaat atgtgccggt 960
gaccaagttg atgacgctgt cgcattggct gatacattga gatcatgtag agctaccgtt 1020
gtcatcggtt tcagagatcc aagagcatta gaaaaggcat ccgttttgtt gagagaacct 1080
agacacgctt tggcagctga catgtacggt agacctacaa tgactgcaga agctagattg 1140
gtcagattag atcctttaga cccaagagca gctgcccaaa ctttggaaca aatacatgcc 1200
gaattgggtg ccatccacca tgctgtcgta ttgcctggtc aatctagaca tgcaccttca 1260
gcttccttga tcgaagttga tgaccaagtt gttgaaagat tcttacacca agaattagtt 1320
ggtactattg ccttagcaag agaattggcc agattttggg aagaatatcc atccggttct 1380
tcaatgcata gagttttatt tgtttccaat cctgacgatc aacaaggtaa ccaatacagt 1440
cacatcttga gagctgcagt tgaacaatta gtcagagtat ggagacacga atccgaatat 1500
gactccgtta accctgcaca ccaacaagaa ggtcaatcct cagcagctgt ttgggctaac 1560
caattaataa gatatgttaa taacgaaatg gctaacttgg acttcacttg cgcctgggtt 1620
gccaagttat taggttccga tagaagaatc taa 1653
<210> 70
<211> 969
<212> DNA
<213> HPDHsi
<400> 70
atgtttatcc acatgaagag tattaacaaa gtagccgtag taggtgctgg tattatcggt 60
gtaggttgga caactttatt attgactaaa ggttacaagg ttaatttgta caccgaaaag 120
aaagaaactt tagaaaaggg tttggcaaag gtatctgcct atttggttaa tttgaaaaat 180
ttgagaatga tagatggtga accaatcgac taccaaagaa atttgacagg tactacaaag 240
attgatgaag ccattcaaaa cgttgaattt gtcatcgaag ctatcatcga agattactcc 300
gctaagaaaa atttgtttaa ttacttggat agtcaattgc ctagagacat cattatagca 360
tcttcaacat ctggtttgtt aatgaccgaa atccaaaagg ctatgaccag atatccagat 420
agaggtgtta tcgcacatcc ttggaatcca cctcacttgt taccattggt agaaattgtt 480
ccaggtgaca aaacctcaaa gtacactttg gatgttacaa aggacttcat ggaaaagttg 540
gatagagttg tcgtagtttt gaagaaagaa gttccaggtt tcattggtaa tagattggcc 600
tttgctttat tcagagaagc agtcaacttg atagatgaag gtgtcgccac tgtagaagat 660
attgacaaag ttatgacagc tgcaataggt ttaagatggg cttttatggg tccattcttg 720
acatatcatt tgggtggtgg tgaaggtggt attgaatact ttttctccaa aggttttggt 780
tatggtgcca atgaatggat gtacaccttg gctaaatggg ataagttccc ttatactgct 840
gttaaaaaga tcgttgatca aatgaaggaa tacgagttta ttaagggtaa aaatttccaa 900
gatttgtcaa gatggagaga tgaaactttg gttagagtat acaaggcagt ttttggtgaa 960
aagagatga 969
<210> 71
<211> 1653
<212> DNA
<213> HPDHsl
<400> 71
atgaatgcaa tgggtaaaac agaaacacaa ggtagattag ctggtaaagt agccttgata 60
actggtgccg ccggtaactt aggtggttct atcgtaagaa gatatttggc cgaaggtgca 120
acagttgtca tgtccggtag agatgctgcc agaactcaag cagctttaga tgcagcagta 180
gctgacgttg gtggtactgc ttcaattgtc actatggatg gtgcaaatat cgaatccgtt 240
agagcaggta tcgcagatgt tgtcgcaaga catggtagaa tagacgtctt ggttaataac 300
gcaggttcag ctggtccaaa gcaagttttg ggtgacttac ctttgttgcc agaagaattg 360
gaaggtacac ctgattcaga aacagttggt gacgcagcaa gaaatataat ggttgtcgca 420
tggaacttag ttagagctgc cgcatctgct atgggtgaag gtggttccat cattaatgtt 480
tccacaatat tctcaagaac tgattatttt ggtagagcag cttatacagt tccaaaagct 540
gcattgaata tcttatccaa aggtatcgca agagaattgg gtaccagagg tatcagagtc 600
aatcaattgt atcctggtcc aatcgcctca gaaagaatca gaacagtttt cgccgcaatg 660
gataagttga aaggtgacga tgccggttca actgccaacc atttctttaa tttgatggct 720
ttggaaagaa gtgtaggtga agatgccaaa gcaaagacct tccctacacc agctgatatc 780
gcaaacactt gcgttttctt gggttcagac gaatccgctg cctttgcagc acacgacttt 840
gaagttacac atggtatggc agtacctttg gaatcaaaat ccgaattttt gcatagacct 900
gacttgagag caatagatgg tgacggtcat ggtatcttga tttcagccgg taaccaatta 960
gacgatgcat tggctatggc aggtttgttc gcagcacaag gttcaaaagt cttgttgggt 1020
tttcacgcac aagctgcatt ggacgatgca gcagaaagat tgccagccgg tgtcaaagca 1080
gttagatttc caagacatga ccatatggca atggacgctg aattggccag attcacagca 1140
catgtagccg caattgacgg tgctattata ttgccaacaa gaggtccagg ttactttcat 1200
ggtgcattga acgaagcttc cgacgctgcc gttgaaagat ttgtcgatga cgaattggaa 1260
ggtgcaattg ctgttgcaag aacattagat agatattggc aacaaggtca agacttgcct 1320
ggtgacccta gagtcttgtt tatcacaaac cctgatgatg gtgcaggtaa tggttgggca 1380
gatgttggta gagcagcaat cgaagaattg ttgagagttt ggagagatga agctagagta 1440
gccgcaacac aaggtaaaac tcctagacct gtttggggta accaaattgt tagatttggt 1500
aataacgaac cagaaaatag agactttgct gcaggtcatg ccgcaagatt gttattaggt 1560
ggtagacaaa tagaaccaat caatttgtac atccctgcat ctatcacaaa agccactggt 1620
gccagaagag ccttagcagg taccgcagaa taa 1653
<210> 72
<211> 867
<212> DNA
<213> HPDHsm
<400> 72
atggctatct taatcactgg tgcctcatca ggtttcggtg ctgctatggc tactgccttc 60
gtccaagctg gttacttagt catcggtaca gctagaagaa ccgaaaaatt agcagatttg 120
catgccaagt taggtgacag atttcaacct ataactatgg atatgacatc aaaggatgac 180
attcaagctg cattagcaca catacaaacc ttgccagaat acttcagaga aatagattgt 240
ttgatcaata acgctggttt ggcattaggt ttggatagag ccgaccaagc tgatttcacc 300
gactgggaaa ctatgatcca aacaaacatc atcggtttaa cctatttgac tagacaaata 360
ttgcctaaga tggttgaaaa taagagaggt tacatcatta acatgggttc aattgctggt 420
aattatccat acgcaggttc caacgtctat ggtgcaacta aggcctacgt tagacaattc 480
tctttgaatt tgagagctga tttgcatggt accggtgtaa gagttactaa tatagaacca 540
ggtttggttg aaggttctga attttcaaac gtcagattca aaggtgacga taagagagcc 600
gctcaattat atgaatacgc aaacgccttg acaagtgaag atatagctca aaccgcatta 660
tggttgtatc aaagacctgc ccacatgaat gttaacacta tcgaattaat gccaactaca 720
caatcttggg ctggtatgca agtttacaaa gatacaccac ctccacctcc acctgttgtc 780
gaaccaaagc ctgccgactt tgctccacaa cctgctccac aagaagaatc cggtttcttt 840
tctaagatca catctttgtt caagtga 867
<210> 73
<211> 957
<212> DNA
<213> HPDHst
<400> 73
atgagacaaa tcaagacagt cagtgttatt ggtgctggtg ttataggtgc tggttggtcc 60
acattattgg cattgaaggg ttacgaaaat tggttttaca ctgaaaagaa agaaacattg 120
gataaaggtt tggctaaaat caagggttac ttaaatgttt tgtatgaata caaattaatt 180
gacaaagaac cagattatta catgcaaaga atacatccaa ccacaaaatt ggacgaagct 240
atttccaata ctgacttcgt cttggaagca attgttgaag aatacggtgc taagaaagcc 300
ttgttcaaac aattggacga aaaattagat aaagacgtta tcttagcatc ttcaacttcc 360
ggtttattga tgactgaaat ccaaaaggca atgacaaagt acccagaaag aggtattata 420
gctcatcctt ggaatccacc tcatttgttg cctttggtcg aaatcgttcc tggtgaaaag 480
acatcacaag acaccatcta cgcaacaaag gattttatgg aaaacaagtt ggaaagagtc 540
gtcgtagttt tgaaaaagga aatcagtggt ttcataggta atagattggc ctttgcattg 600
ttcagagaag cagtttactt ggttgatgaa ggtattgcca ctgttgaaga tattgataaa 660
gttatgacag ctgcaatcgg tttaagatgg gccttcatgg gtccattttt gacttatcac 720
ttaggtggtg gtgaaggtgg tttggaatat ttcttttcca gaggtttcgg ttatggtgct 780
aacgaatgga tgcatacttt agcaaagtat gataagttcc catatactgg tgttgtaaag 840
tcagttcaac aaatgaaaga atatgaattt gtcaaatcca agacattcca agaattgagt 900
agatggagag atgaaaagtt gataagtttg ataaagttgt tgagaggtaa aatataa 957
<210> 74
<211> 480
<212> PRT
<213> AADHab
<400> 74
Met Glu Ile Asn Glu Lys Asp Leu Lys Leu Ile Ile Glu Asn Val Leu
1 5 10 15
Asp Lys Leu Glu Ser Asp Lys Ser Asp Lys Lys Ile Phe Asp Ser Ser
20 25 30
Leu Asn Ile Pro Ser Ser Gly Gln Asp Gly Ile Phe Glu Glu Val Asn
35 40 45
Ala Ala Val Asp Ala Ala Glu Ala Ala His Leu Glu Leu Met Lys Leu
50 55 60
Thr Leu Glu Lys Arg Arg Glu Ile Ile Arg Ser Ile Arg Lys Ile Ile
65 70 75 80
Met Ser Asn Leu Glu Glu Ile Ser Lys Leu Ala Val Glu Glu Thr Thr
85 90 95
Phe Gly Arg Val Glu Asp Lys Ile Glu Lys Asn Arg Leu Ala Ala Leu
100 105 110
Lys Thr Pro Gly Ile Glu Asp Leu Glu Pro Thr Ala Tyr Ser Asp Asp
115 120 125
Asn Gly Met Thr Leu Met Glu Arg Ala Ala Tyr Gly Val Ile Gly Ser
130 135 140
Ile Ile Pro Ser Thr Asn Pro Thr Ser Ser Val Ile Asn Asn Gly Ile
145 150 155 160
Ser Met Ile Ala Gly Gly Asn Ser Val Val Phe Asn Pro His Pro Ala
165 170 175
Ala Lys Arg Ser Ser Cys Phe Thr Ile Ser Leu Ile Asn Gln Ala Ile
180 185 190
Val Lys Ala Gly Gly Pro Pro Asn Val Leu Cys Ala Ile Ala Asn Pro
195 200 205
Thr Ile Asp Ser Ala Gln Thr Leu Met Lys His Pro Lys Ile Lys Leu
210 215 220
Leu Val Val Thr Gly Gly Pro Ala Val Val Lys Thr Ala Met Asn Ser
225 230 235 240
Asp Lys Lys Val Ile Ala Ala Gly Pro Gly Asn Pro Pro Cys Val Val
245 250 255
Asp Glu Thr Ala Asp Leu Val Lys Ala Gly Arg Asp Ile Val Asn Gly
260 265 270
Ala Gly Phe Asp Asn Asn Ile Val Cys Ile Cys Glu Lys Glu Ile Leu
275 280 285
Ala Val Ser Gln Ile Ala Asp Gln Leu Lys Glu Glu Met Ile Lys Asn
290 295 300
Gly Ala His Glu Leu Lys Gly Glu Gln Ile Glu Lys Val Thr Lys Leu
305 310 315 320
Val Ile Thr Asp Pro Gly Lys Pro Gly His Glu Gly Ala Pro Asn Lys
325 330 335
Lys Tyr Val Gly Lys Asn Ala Ser Val Ile Ala Arg Asp Ile Gly Leu
340 345 350
Asp Ile Ser Glu Gln Thr Lys Ile Leu Leu Cys Glu Val Asp Arg Tyr
355 360 365
His Pro Leu Val Trp Thr Glu Gln Leu Leu Pro Val Ile Pro Leu Val
370 375 380
Arg Phe Asn Asn Val Asp Glu Ala Ile Asp Phe Ala Val Gln Cys Glu
385 390 395 400
His Asn Phe Arg His Ser Ala Ser Ile His Ser Lys Asn Ile Ala Thr
405 410 415
Leu Ser Arg Met Ala Lys Leu Met Asn Cys Ser Leu Phe Ile Lys Asn
420 425 430
Gly Pro Asn Tyr Ser Gly Leu Gly Phe Gly Gly Ala Gly Tyr Thr Ser
435 440 445
Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Leu Thr Arg Ala Arg Thr
450 455 460
Phe Thr Arg Glu Arg Arg Cys Val Leu Val Gly Tyr Phe Arg Ile Val
465 470 475 480
<210> 75
<211> 477
<212> PRT
<213> AADHal
<400> 75
Met Val Asp Gln Thr Leu Ile Glu Gln Ile Thr Arg Ala Val Leu Thr
1 5 10 15
Gln Met Lys Ala Gly Lys Asp Ala Ala Val Ser Gly Asp Gly Ile Phe
20 25 30
Ala Thr Val Asp Gln Ala Val Ala Ala Ala Arg Gln Ala Tyr Gln Glu
35 40 45
Leu Arg Leu Leu Thr Leu Glu Lys Arg Glu Thr Leu Ile Arg Ala Ile
50 55 60
Arg Asp Ala Ala Phe Ala Asn Ala Ala Val Ile Ala Gln Met Ala Val
65 70 75 80
Gln Glu Ser Gly Met Gly Arg Val Glu Asp Lys Ile Leu Lys Asn Gln
85 90 95
Leu Ala Ala Arg Lys Thr Pro Gly Thr Glu Asp Leu Thr Ser Arg Ala
100 105 110
Trp Ser Gly Asp His Gly Leu Thr Leu Val Glu Met Ala Pro Tyr Gly
115 120 125
Val Ile Gly Ala Ile Thr Pro Thr Thr Asn Pro Ser Glu Thr Val Ile
130 135 140
Cys Asn Ser Ile Gly Met Ile Ala Ala Gly Asn Ser Ile Val Phe Ser
145 150 155 160
Pro His Pro Thr Ala Gln Asn Thr Ser Leu Thr Thr Ile Arg Leu Leu
165 170 175
Asn Glu Ala Ile Val Lys Ala Gly Gly Pro Asp Asn Leu Leu Thr Ala
180 185 190
Val Ala Glu Pro Ser Ile Glu Ala Ala Asn Ala Met Met Arg His Pro
195 200 205
Gly Ile Gln Met Leu Val Ala Thr Gly Gly Pro Ala Val Val Lys Ala
210 215 220
Val Leu Ser Ser Gly Lys Lys Ala Ile Gly Ala Gly Ala Gly Asn Pro
225 230 235 240
Pro Ala Val Val Asp Glu Thr Ala Asp Ile Ala Lys Ala Ala Lys Asp
245 250 255
Ile Val Ala Gly Cys Ser Phe Asp Asn Asn Leu Pro Cys Ile Ala Glu
260 265 270
Lys Glu Ile Ile Ala Val Gly Arg Ile Ala Asp Glu Leu Ile Ser Tyr
275 280 285
Leu Gln Lys Tyr Gly Ala Tyr Leu Ile Ser Gly Arg Asp Ile Glu Arg
290 295 300
Leu Met Glu Val Val Leu Thr Glu Arg Thr Glu Glu Met Ala Pro Gly
305 310 315 320
Cys Val Gly Lys Pro Arg Arg Val Tyr Gly Val Asn Lys Asp Tyr Ile
325 330 335
Gly Lys Asp Ala Lys Phe Ile Leu Ser Lys Ile Asn Ile Gln Ala Pro
340 345 350
Asp His Ile Arg Val Ile Leu Cys Glu Thr Pro Ala Asp His Pro Phe
355 360 365
Val Leu Glu Glu Leu Met Met Pro Val Leu Pro Leu Val Ser Val Arg
370 375 380
Asp Ile Asp Ala Ala Ile Asp Leu Ala Val Lys Val Glu His Gly Asn
385 390 395 400
Arg His Thr Ala Val Met His Ser Lys Asn Val Asp Tyr Met Thr Arg
405 410 415
Leu Ala Lys Ala Ile Glu Thr Thr Ile Phe Val Lys Asn Ala Pro Ser
420 425 430
Tyr Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr Phe Thr Ile
435 440 445
Ala Gly Pro Thr Gly Glu Gly Leu Thr Ser Ala Arg Ser Phe Thr Arg
450 455 460
Gln Arg Arg Cys Ala Leu Val Asp Ala Phe Ser Ile Val
465 470 475
<210> 76
<211> 464
<212> PRT
<213> AADHbs
<400> 76
Met Asp Glu Ile Met Ile Lys Asn Ile Val Arg Glu Val Leu Met Lys
1 5 10 15
Thr Asn Leu Asp Ile Ser Cys Lys Ser Asp Gln Gly Val Phe Glu Asp
20 25 30
Val Asn Asp Ala Ile Asp Ala Ala Tyr Ile Ala Gln Arg Asp Trp Tyr
35 40 45
Met Asn Tyr Lys Val Glu Asp Arg Arg Arg Ile Ile Glu Ala Ile Arg
50 55 60
Val Val Ala Arg Glu Asn Ala Pro Met Phe Ala Lys Met Ile His Glu
65 70 75 80
Glu Thr Gly Met Gly Arg Tyr Glu Asp Lys Ile Thr Lys Asn Leu Ala
85 90 95
Val Ile Asp Lys Thr Pro Gly Val Glu Cys Leu Val Thr Asp Ala Ile
100 105 110
Ser Gly Asp Ser Gly Leu Met Ile Glu Glu Gln Ala Pro Phe Gly Val
115 120 125
Ile Gly Ala Ile Thr Pro Ser Thr Asn Pro Thr Glu Thr Ile Ile Asn
130 135 140
Asn Thr Ile Ser Met Ile Gly Gly Gly Asn Ala Val Val Phe Asn Val
145 150 155 160
His Pro Gly Ala Lys Lys Val Cys Ala Val Cys Leu Gln Ile Leu His
165 170 175
Lys Thr Ile Val Glu Asn Gly Gly Pro Ala Asn Leu Ile Thr Met Gln
180 185 190
Arg Lys Pro Asp Met Glu Ala Val Asn Lys Leu Thr Ala Ser Pro Lys
195 200 205
Ile Arg Leu Met Val Gly Thr Gly Gly Met Gly Met Val Asn Ala Leu
210 215 220
Leu Lys Ser Gly Lys Lys Thr Ile Gly Ala Gly Ala Gly Asn Pro Pro
225 230 235 240
Val Val Val Asp Asp Thr Ala Asp Leu Asp Lys Ala Ala Ser Glu Ile
245 250 255
Tyr Arg Gly Ala Ser Phe Asp Asn Asn Leu Leu Cys Leu Ala Glu Lys
260 265 270
Glu Thr Phe Val Met Asp Asn Val Ala Asp Glu Leu Ile Arg Lys Met
275 280 285
Cys Ala Cys Gly Ala His Leu Ile Thr Pro Gln Glu Thr Glu Gln Leu
290 295 300
Leu Lys Val Ile Phe Leu Glu Lys Asp Gly Lys Tyr Ser Val Asn Lys
305 310 315 320
Lys Trp Val Gly Lys Asp Ala Ser Leu Ile Leu Glu Ser Ile Gly Ile
325 330 335
Lys Asp Ala Asp Thr Arg Leu Val Leu Cys Glu Val Pro His Asp His
340 345 350
Pro Phe Val Leu Val Glu Gln Leu Met Pro Ile Met Pro Ile Val Arg
355 360 365
Cys Lys Thr Phe Glu Asp Cys Val Lys Tyr Ala Val Val Ala Glu Asn
370 375 380
Gly Asn Arg His Thr Ala Ser Met Phe Ser Lys Asn Val Asp His Met
385 390 395 400
Thr Arg Phe Ala Lys Glu Ile Glu Thr Thr Ile Phe Thr Lys Asn Ala
405 410 415
Ala Thr Leu Val Gly Val Gly Ile Gly Gly Glu Gly His Thr Thr Met
420 425 430
Thr Ile Ala Gly Pro Thr Gly Glu Gly Leu Thr Cys Ala Arg Ser Phe
435 440 445
Thr Arg Arg Arg Arg Cys Met Leu Ala Asp Gly Gly Leu Arg Val Ile
450 455 460
<210> 77
<211> 484
<212> PRT
<213> AADHbw
<400> 77
Met Asp Val Arg Gln Gln Asp Val Glu Arg Ile Val Val Glu Val Leu
1 5 10 15
Lys Lys Met Met Ser Asp Gln Pro Thr Ala Ala Ala Thr Thr Val Val
20 25 30
Ala Ala Ser Gly Cys Asp Cys Gly Asp Phe Gly Leu Phe Asp Arg Leu
35 40 45
Glu Asp Ala Val Gln Ala Ala Glu Ala Ala Gln Lys Lys Ile Ser Thr
50 55 60
Val Ala Met Arg Asp Lys Ile Ile Ala Ala Ile Arg Lys Ala Gly Leu
65 70 75 80
Glu Asn Ala Lys Ala Phe Ala Glu Ile Ala His Asn Glu Thr Gly Met
85 90 95
Gly Arg Val Ser Asp Lys Ile Ala Lys Asn Ile Leu Val Cys Glu Arg
100 105 110
Thr Pro Gly Thr Glu Cys Leu Ser Pro Met Ala Ile Ser Gly Asp Met
115 120 125
Gly Leu Thr Leu Ile Glu Asn Ala Pro Trp Gly Val Ile Ala Ser Val
130 135 140
Thr Pro Ser Thr Asn Pro Thr Ala Thr Val Ile Asn Asn Ala Ile Ser
145 150 155 160
Met Ile Ala Gly Gly Asn Ser Val Ile Phe Ala Pro His Pro Asn Ala
165 170 175
Lys Arg Ala Ser Gln Thr Ala Ile Gln Val Leu Asn Lys Ala Ile Ile
180 185 190
Glu Ala Thr Gly Val Ala Asn Leu Leu Val Ala Val Lys Glu Pro Thr
195 200 205
Ile Glu Val Ala Gln Glu Leu Phe Ser His Pro Arg Ile Lys Leu Leu
210 215 220
Val Val Thr Gly Gly Glu Ala Val Val Ala Gln Ala Arg Lys Val Ala
225 230 235 240
Thr Met Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro Pro Val Val Val
245 250 255
Asp Glu Thr Ala Asn Ile Ala Arg Ala Ala Arg Ser Ile Tyr Asp Gly
260 265 270
Ala Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu Lys Glu Ile Ile
275 280 285
Ala Val Asp Ser Ile Ala Asp Gln Leu Lys Ala Glu Met Lys Ala Ile
290 295 300
Gly Ala Val Glu Ile Ser Leu Glu Gln Ala Asp Ala Val Ala Arg Val
305 310 315 320
Val Leu Arg Asn Tyr Pro Gln Val Glu Gly Gly Lys Ala Pro Asn Pro
325 330 335
Asn Pro Lys Trp Val Gly Arg Asp Ala Ala Leu Ile Ala Lys Ala Ala
340 345 350
Gly Ile Asp Val Pro Asp Ser Cys Arg Leu Leu Ile Val Asp Val Lys
355 360 365
Arg Asp Ile Asn His Val Phe Ala Arg Val Glu Gln Leu Met Pro Val
370 375 380
Ile Pro Leu Leu Arg Ala Ala Asn Val Asp Glu Ala Ile Glu Trp Ala
385 390 395 400
Leu Ile Leu Glu Arg Gly Leu Ser His Thr Ala Gly Met His Ser Arg
405 410 415
Asn Ile Asp Asn Met Asp Lys Met Ala Arg Ala Met Asn Thr Ser Leu
420 425 430
Phe Val Lys Asn Gly Pro His Leu Ala Ala Leu Gly Ala Gly Gly Glu
435 440 445
Gly Trp Thr Thr Met Thr Ile Ser Thr Pro Thr Gly Glu Gly Val Thr
450 455 460
Cys Ala Arg Ser Phe Val Arg Leu Arg Arg Cys Cys Val Val Asp Asn
465 470 475 480
Phe Arg Ile Val
<210> 78
<211> 437
<212> PRT
<213> AADHcs
<400> 78
Met Lys Ser Ser Ile Tyr Ser Ser Val Ser Glu Ala Val Met Ala Ala
1 5 10 15
Arg Gly Ala Phe Asn Arg Tyr Ala Asp Leu Thr Leu Asn Glu Arg Lys
20 25 30
Glu Ile Ile Glu Ala Val Lys Ala Ala Val Arg Pro Arg Ile Thr Gln
35 40 45
Leu Ala Glu Met Thr Ala Ala Glu Thr Gly Met Gly Asn Val Ala Asp
50 55 60
Lys Ile Glu Lys Ile Arg Leu Ala Val Glu Lys Thr Pro Gly Val Glu
65 70 75 80
Asp Leu Ile Thr Glu Val Asn Thr Gly Asp His Gly Met Thr Leu Tyr
85 90 95
Glu Leu Ser Ala Phe Gly Val Val Cys Ala Val His Pro Cys Thr Asn
100 105 110
Pro Cys Ala Thr Leu Ile Ser Asn Thr Ile Gly Ile Leu Ala Ala Gly
115 120 125
Asn Ala Val Val His Cys Pro His Pro Arg Ala Val Arg Val Ser Gln
130 135 140
Tyr Leu Thr Gly Met Ile Ser Asp Ala Val Arg Asp Val Cys Gly Ile
145 150 155 160
Asp Asn Leu Val Val Thr Val Gly Glu Ser Leu Met Gly Ser Thr Arg
165 170 175
Glu Ile Met Ser His Pro Asp Val Asp Leu Ile Val Cys Thr Gly Gly
180 185 190
Ser Gly Pro Leu Arg Gln Ala Met Ser Ala Gly Lys Lys Val Ile Gly
195 200 205
Ala Gly Pro Ala Asn Pro Val Ala Ile Val Asp Glu Thr Ala Asp Leu
210 215 220
Glu Arg Ala Ala Arg Asp Ile Val Lys Gly Ala Ser Phe Asp Asn Asn
225 230 235 240
Leu Met Cys Thr Ser Glu Lys Ser Ile Val Ala Val Glu Lys Ile Ala
245 250 255
Asp Glu Leu Ile Ser His Leu Glu Arg Asn Gly Val Tyr Tyr Thr Arg
260 265 270
Ser Ala Glu Glu Ile Asp Arg Leu Tyr Cys Thr Ala Val Thr Glu Asp
275 280 285
Phe Val Ile Asn Lys Lys Met Glu Gly Gln Ser Ala Asp Val Ile Leu
290 295 300
Asp Ala Ala Lys Ile Pro His Arg Lys Gly Ile Lys Leu Ile Val Ala
305 310 315 320
Glu Thr Asp Arg Thr His Pro Phe Ala Thr Leu Glu Leu Leu Met Pro
325 330 335
Leu Leu Pro Leu Val Arg Ala Arg Asp Phe Asp Glu Ala Leu Glu Ala
340 345 350
Ala Leu Asp Ile Glu Gln Gly Phe Arg His Thr Ala Thr Ile His Ser
355 360 365
Glu Ser Val Glu Arg Met Asn Arg Ala Ala Lys Lys Leu Gln Thr Ala
370 375 380
Val Phe Val Lys Asn Gly Pro Ser Leu Leu Gly Ile Gly Phe Asp Lys
385 390 395 400
Glu Gly His Thr Ser Phe Thr Ile Gly Thr Ile Thr Gly Glu Gly Thr
405 410 415
Thr Ser Ala Arg His Phe Ala Arg Arg Arg Arg Cys Thr Leu Thr Ser
420 425 430
Gly Phe Ser Ile Arg
435
<210> 79
<211> 485
<212> PRT
<213> AADHho
<400> 79
Met Ala Leu Arg Glu Asp Arg Ile Ala Glu Ile Val Glu Arg Val Leu
1 5 10 15
Ala Arg Leu Asp Gly Asn Ser Gly Ser Ser Ala Ala Pro His Ser Gly
20 25 30
Ser Gly Ala Pro Ala Ala Thr Ala Gly Gly Ala Ser Leu Asp Ile Pro
35 40 45
Arg Gly Thr Leu Gly Val Tyr Ala Asp Ala Asp Ala Ala Val Asn Ala
50 55 60
Ala Arg Arg Gly Phe Ala Ala Asn Glu Ala Leu Pro Leu Arg Thr Arg
65 70 75 80
Gln Ala Met Ile Asp Ala Met Arg Lys Val Ala Arg Ala His Ile Pro
85 90 95
Glu Leu Ala Arg Tyr Ala Val Ala Glu Thr Gly Leu Gly Arg Tyr Glu
100 105 110
Asp Lys Leu Ala Lys Asn Glu Leu Val Ile Ala Lys Thr Pro Gly Pro
115 120 125
Glu Ile Leu Ala Pro Val Ala Tyr Thr Gly Asp Asp Gly Leu Thr Leu
130 135 140
Thr Glu Arg Ala Pro Tyr Gly Val Ile Gly Ala Ile Thr Pro Cys Thr
145 150 155 160
Asn Pro Thr Glu Thr Val Ile Cys Asn Ala Ile Gly Met Leu Ser Gly
165 170 175
Gly Asn Ala Val Val Phe Asn Val His Pro Ser Ala Ala Arg Val Cys
180 185 190
Asn Trp Leu Val His Leu Leu Asn Glu Ala Ile Met Ser Val Gly Gly
195 200 205
Pro Arg Asp Ala Ile Thr Ser Val Glu Ser Pro Thr Ile Asp Ser Ala
210 215 220
Gln Thr Leu Met Thr His Ala Gly Val Arg Leu Val Val Val Thr Gly
225 230 235 240
Gly Pro Gly Val Val Arg Ala Ala Met Lys Ser Gly Lys Lys Val Ile
245 250 255
Ala Ala Gly Pro Gly Asn Pro Pro Ala Val Val Asp Glu Thr Ala Asn
260 265 270
Leu Ala Lys Ala Ala Ala Ala Ile Ile Lys Gly Ala Ser Ile Asp Asn
275 280 285
Asn Ile Ile Cys Thr Ala Glu Lys Glu Ile Val Ala Val Ala Ser Ile
290 295 300
Ala Asp Glu Leu Ser Arg Leu Leu Gly Gln Arg Gly Ala Leu Val Leu
305 310 315 320
Gly Asp Ala Gln Val Arg Ala Leu Glu Arg Val Val Leu Asp Gly Glu
325 330 335
His Val Asn Lys Glu Trp Val Gly Lys Asp Ala Ser Arg Ile Ala Glu
340 345 350
Gln Ile Gly Leu Arg Gly His Gly Ser Asp Leu Arg Leu Leu Val Cys
355 360 365
Pro Val Asp Glu Gly His Pro Phe Val Gln His Glu Leu Leu Met Pro
370 375 380
Val Ile Gly Leu Val Arg Val Ser Asp Ala Thr Glu Ala Met Ala Thr
385 390 395 400
Ala Val Arg Val Glu His Gly Phe Cys His Thr Ala Val Met His Ser
405 410 415
Thr His Ile Asp Arg Leu Ser Ala Met Ala Arg Val Cys Asn Ala Ser
420 425 430
Ile Phe Val Lys Asn Asp Cys Asn Leu Ala Gly Leu Gly Leu Gly Gly
435 440 445
Glu Gly Phe Thr Ser Phe Thr Ile Ala Ser Pro Thr Gly Glu Gly Leu
450 455 460
Thr Thr Ala Arg Asp Phe Thr Arg Val Arg Arg Cys Thr Leu Lys Glu
465 470 475 480
Ser Phe Arg Phe Val
485
<210> 80
<211> 467
<212> PRT
<213> AADHhs
<400> 80
Met Lys Ile Lys Glu Asn Glu Leu Asp Lys Ile Val Asn Gln Val Ile
1 5 10 15
Ser Ser Leu Asn Asn Lys Gln Asn Ser Asn Asp Phe Asn Thr Lys Ile
20 25 30
Asn Tyr Gly Ile Phe Ser Thr Met Asp Glu Ala Ile Ala Glu Ala Val
35 40 45
Lys Ala Gln Ala Cys Leu Gln Leu Asn Tyr Ser Thr Glu Ala Arg Glu
50 55 60
Lys Ile Ile Lys Ser Ile Arg Lys Asn Val Ser Lys His Val Glu Lys
65 70 75 80
Ile Ser Glu Met Ala Val Glu Glu Thr Asp Met Gly Arg Ile Glu Asp
85 90 95
Lys Ile Ile Lys Asn Asn Leu Ala Ile Asn Lys Thr Pro Gly Thr Glu
100 105 110
Asp Leu Arg Thr Glu Ala Phe Ser Gly Lys Lys Gly Leu Thr Ile Val
115 120 125
Glu Glu Ala Pro Phe Gly Val Ile Cys Ser Ile Ala Pro Val Thr Asn
130 135 140
Pro Thr Glu Thr Ile Ile Ser Asn Ala Ile Ser Met Ile Ala Ser Cys
145 150 155 160
Asn Gly Val Val Phe Asn Ser His Pro Gly Ala Lys Lys Val Ser Lys
165 170 175
Tyr Ile Ile Glu Val Leu Asn Lys Val Ile Met Glu Ala Gly Gly Pro
180 185 190
Glu Asn Leu Leu Thr Ala Val Asn Glu Pro Thr Leu Gln Thr Val Glu
195 200 205
Ser Cys Met Arg Asp Asp Arg Ile Ala Met Ile Val Ala Thr Gly Gly
210 215 220
Pro Gly Val Val Asn Ala Ala Leu Ser Ser Gly Lys Lys Ala Ile Gly
225 230 235 240
Ala Gly Ala Gly Asn Pro Pro Val Leu Val Asp Asp Thr Val Asp Leu
245 250 255
Lys Arg Val Ala Lys Asp Ile Ile Asn Gly Ala Ser Phe Asp Asn Asn
260 265 270
Leu Pro Cys Thr Ser Glu Lys Ala Ile Val Ala Leu Glu Ser Ile Ala
275 280 285
Asp Ser Leu Leu Asn Glu Met Thr Asn Gln Asn Ala Gln Leu Val His
290 295 300
Asp Ile Lys Ala Leu Glu Arg Val Ile Leu Asn Asp Asp Gly Ser Ile
305 310 315 320
Asn Lys Ala Leu Val Gly Lys Asp Ala Ala Phe Ile Leu Asn Lys Ala
325 330 335
Gly Leu Lys Ala Lys Ser Glu Asp Leu Arg Leu Val Ile Val Asp Val
340 345 350
Asp Leu Arg His Pro Phe Val Gln Lys Glu Gln Leu Met Pro Val Ile
355 360 365
Pro Leu Val Arg Ala Lys Asn Phe Asn Glu Ala Met Glu Met Gly Val
370 375 380
Asp Ile Glu Glu Gly Asn Arg His Thr Ala Ile Ile His Ser Lys Asn
385 390 395 400
Val Asp Asn Leu Thr Lys Phe Ala Lys Lys Ile Glu Thr Thr Ile Tyr
405 410 415
Val Lys Asn Ala Pro Ser Tyr Ala Gly Ile Gly Ala Gly Gly Glu Gly
420 425 430
Tyr Ala Thr Phe Thr Ile Ala Gly Pro Thr Gly Glu Gly Leu Thr Ser
435 440 445
Ala Arg Ser Phe Thr Arg Lys Arg Arg Cys Val Leu Val Asp Gly Phe
450 455 460
Ser Ile Ile
465
<210> 81
<211> 465
<212> PRT
<213> AADHma1
<400> 81
Met Gln Thr Asp Ala Gln Gln Ile Glu Ser Ile Val Arg Arg Val Ile
1 5 10 15
Glu Gln Leu His Ser Pro Gln Arg Asp Gly Glu Ser Tyr Gly Val Phe
20 25 30
Arg Thr Leu Asp Asp Ala Val Ala Gly Ala Gln Gly Ala Tyr Lys Lys
35 40 45
Ile Arg Thr Met Ala Gln Arg Glu Ala Ile Ile Ala Ala Ile Arg Arg
50 55 60
Thr Gly Ser Glu Asn Val Gln Ala Leu Ser Glu Leu Ala Val Gln Glu
65 70 75 80
Thr Gly Phe Gly Arg Val Glu Asp Lys Ile Arg Lys His Arg Leu Val
85 90 95
Leu Asp Lys Thr Pro Gly Ile Glu Ala Ile Val Pro Met Ala Val Thr
100 105 110
Gly Asp His Gly Leu Ser Leu Ile Glu Asn Ala Pro Trp Gly Val Ile
115 120 125
Ala Ser Val Thr Pro Ser Thr Asn Pro Ser Ala Thr Ile Leu Asn Asn
130 135 140
Ala Ile Ser Met Ile Ala Ala Gly Asn Ser Val Val Phe Ser Pro His
145 150 155 160
Pro Ala Ala Arg Ala Val Ser Gln Arg Thr Ile Gln Leu Ile Asn Arg
165 170 175
Ala Ser Val Ser Ala Gly Gly Pro Ala Asn Leu Val Thr Cys Val Glu
180 185 190
Glu Pro Thr Ile Glu Ala Ala Thr Arg Leu Phe Ser Phe Pro Gly Ile
195 200 205
Gln Leu Leu Thr Ile Thr Gly Gly Glu Gly Val Val Asn Ala Ala Arg
210 215 220
Lys Val Thr Asp Lys Arg Leu Ile Ala Ala Gly Pro Gly Asn Pro Pro
225 230 235 240
Val Val Val Asp Glu Thr Ala Asp Ile Glu Arg Ala Ala Ile Ser Ile
245 250 255
Val Gln Gly Ala Ser Phe Asp Asn Asn Ile Ile Cys Val Asp Glu Lys
260 265 270
Glu Ile Ile Ala Val Glu Ser Ile Ala Thr Glu Leu Lys Thr Ala Met
275 280 285
Cys Arg His Gly Ala Ala Glu Ile Asn Ala Asp Gln Ala Asp Ala Val
290 295 300
Ala Arg Leu Val Leu Ala Gly Tyr Pro Gly Pro Asn Pro His Pro Lys
305 310 315 320
Pro Glu Trp Val Gly Arg Asp Ala Glu Lys Ile Ala Ala Ala Ala Gly
325 330 335
Phe Ser Val Pro Ala Gly Thr Arg Leu Leu Val Thr Glu Thr Glu Arg
340 345 350
Asp His Ala Phe Ala Thr Thr Glu Met Met Leu Pro Val Ile Ser Leu
355 360 365
Ile Arg Ala Arg Asp Ala Asp Gln Ala Ile Asp Trp Ala Val Glu Leu
370 375 380
Glu Ala Gly Asn Arg His Thr Ala Ala Met His Ser Arg Asn Ile Asp
385 390 395 400
Asn Leu Ser Arg Met Gly Leu Glu Ile Asn Cys Ser Leu Phe Val Lys
405 410 415
Asn Gly Pro Cys Leu Ala Gly Leu Gly Ala Gly Gly Glu Gly Trp Thr
420 425 430
Ser Met Thr Ile Ser Thr Pro Thr Gly Glu Gly Val Thr Asn Ala Ser
435 440 445
Thr Phe Val Arg Lys Arg Arg Cys Thr Met Val Asp Ser Phe Arg Ile
450 455 460
Val
465
<210> 82
<211> 473
<212> PRT
<213> AADHma2
<400> 82
Met Ala Phe Asn Glu Asp Leu Ile Ala Ser Ile Val Glu Gln Val Leu
1 5 10 15
Lys Glu Ile Gln Asp Asp Asp His Val Ile Ala Ser Ala Arg Gln Ser
20 25 30
Val Val Lys Ala Asn Asp Asn Trp Gly Val Tyr Thr Asp Val Asp Glu
35 40 45
Ala Met Glu Val Val Asn Lys Ala Gln Lys Glu Phe Ala Ala Met Pro
50 55 60
Ile Ser Lys Arg Asp Glu Ile Ile Ser Ala Ile Arg Thr Ala Gly Val
65 70 75 80
Glu Asn Ala Glu His Leu Ala Arg Ile Ala His Glu Val Thr Gly Tyr
85 90 95
Gly Arg Ile Glu Asp Lys Thr Val Lys Asn Arg Asn Ala Ala Met Leu
100 105 110
Thr Pro Gly Ile Glu Asp Leu Gln Lys Glu Phe Leu Ser Gly Asp Gly
115 120 125
Gly Ser Val Ile Val Glu Tyr Gly Pro Met Gly Val Ile Ala Ser Leu
130 135 140
Met Pro Ser Thr His Pro Thr Ala Phe Val Ile Asn His Ala Ile Ala
145 150 155 160
Met Leu Ala Ala Gly Asn Ala Ile Phe Ile Cys Pro His Pro Lys Ala
165 170 175
Gln Ala Ala Thr Leu Glu Ala Met Lys Ala Leu Asn Lys Ala Ile Val
180 185 190
Glu Ala Gly Gly Pro Lys Tyr Leu Met Val Ala Val Asp Lys Ala Asp
195 200 205
Met Glu Thr Ser Asn Lys Val Tyr Ala His Pro Tyr Val Lys Met Val
210 215 220
Ala Ala Ala Gly Gly Glu Gly Val Ile Arg Ala Ala Leu Lys Ser Gly
225 230 235 240
Lys Lys Ala Ile Thr Gly Gly Pro Gly Asn Pro Pro Val Val Val Asp
245 250 255
Glu Thr Ala Asp Ile Ala Lys Ala Ala Lys Asp Ile Ile Asp Gly Glu
260 265 270
Ser Tyr Asp Asn Asn Val Leu Cys Ile Ala Glu Lys Glu Val Phe Val
275 280 285
Val Glu Ser Val Val Asp Arg Leu Ile Ala Glu Met Gln Lys Asn Asn
290 295 300
Ala Tyr Leu Ile Lys Gly Gln Asp Val Ala Asn Leu Thr Gly Val Thr
305 310 315 320
Ile Lys Asp Gly Lys Pro Asn Gly Gln Leu Val Gly Lys Asn Pro Ser
325 330 335
Val Ile Leu Lys Glu Ile Gly Ile Glu Val Gly Asp Asp Val Arg Thr
340 345 350
Val Ile Phe Glu Ala Pro Pro Glu His Pro Leu Val Met Ile Glu Gln
355 360 365
Leu Met Pro Val Leu Pro Ile Val Arg Val Lys Asp Phe Asp Glu Ala
370 375 380
Val Lys Trp Ala Val Lys Ala Glu His Gly Asp Gly His Thr Ala Ile
385 390 395 400
Leu His Ser Asn Asn Ile Asn Arg Ile Thr Glu Tyr Ala Arg Ala Met
405 410 415
Gln Val Thr Val Leu Val Val Asn Ala Pro Ser Tyr Ser Cys Met Asp
420 425 430
Pro Gln Ser Lys Thr Leu Val Tyr Ala His Thr Leu Thr Gly Pro Thr
435 440 445
Gly Glu Gly Phe Cys Thr Pro Arg His Phe Thr Arg Gln Glu Arg Ile
450 455 460
Val Leu Gly Gly Ala Phe His Phe Val
465 470
<210> 83
<211> 478
<212> PRT
<213> AADHmm
<400> 83
Met Asp Gln Lys Glu Ile Glu Asn Val Val Lys Ala Val Leu Ala Ser
1 5 10 15
Met Ser Ala Gly Thr Gln Pro Ala Ala Ala Ser Ala Ala Pro Gln Gln
20 25 30
Ala Ala Ala Ser Gln Asn Asn Gly Phe Gly Val Phe Glu Ser Leu Asp
35 40 45
Asp Ala Val Leu Ala Ala Lys Glu Ala Gln Lys Ser Leu Lys Thr Val
50 55 60
Glu Met Arg Asn Leu Cys Ile Gly Ala Ile Arg Arg Ala Ala Thr Glu
65 70 75 80
His Ala Arg Glu Leu Ala Val Leu Ala Val Glu Glu Thr Gly Met Gly
85 90 95
Arg Val Glu Asp Lys Leu Ala Lys Asn Leu Ala Gln Ala Asn Gly Thr
100 105 110
Pro Gly Val Glu Cys Leu Arg Pro Glu Val Leu Thr Gly Asp His Gly
115 120 125
Leu Thr Leu Ile Glu Asn Ala Ala Trp Gly Val Ile Ala Ser Val Thr
130 135 140
Pro Ser Thr Asn Pro Ala Ala Thr Ala Ile Asn Asn Ala Ile Ser Met
145 150 155 160
Ile Ala Gly Gly Asn Ser Val Ile Phe Ala Pro His Pro Ala Ala Lys
165 170 175
Lys Val Ser Gln Arg Thr Ile Thr Ile Leu Asn Glu Ala Ile Val Ala
180 185 190
Ala Gly Gly Pro Asn Asn Leu Leu Val Thr Val Ala Lys Pro Asp Ile
195 200 205
Glu Thr Ala Gln Arg Leu Phe Lys Tyr Pro Gly Ile Gly Leu Leu Val
210 215 220
Val Thr Gly Gly Asp Ala Val Val Glu Ser Ala Arg Lys His Thr Asn
225 230 235 240
Lys Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro Pro Val Val Val Asp
245 250 255
Glu Thr Ala Asp Ile Glu Arg Ala Ala Lys Ala Ile Val His Gly Ala
260 265 270
Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu Lys Val Leu Ile Ala
275 280 285
Val Asp Cys Ile Ala Asp Lys Leu Ile Thr Glu Met Gln Arg Asn His
290 295 300
Ala Val Leu Leu Thr Arg Glu Gln Ser Glu Lys Leu Ile Pro Val Leu
305 310 315 320
Leu Lys Asn Val Asp Glu Thr Gly His Gly Thr Val Ser Arg Asp Trp
325 330 335
Val Gly Arg Asp Ala Ala Lys Ile Ala Ala Ala Ile Gly Met Thr Val
340 345 350
Pro Ala Asp Thr Arg Leu Leu Ile Ala Glu Thr Asp Cys Lys His Pro
355 360 365
Phe Ala Val Thr Glu Leu Met Met Pro Val Leu Pro Ile Ile Arg Val
370 375 380
Lys Asp Val Asp Gln Ala Ile Asp Leu Ala Val Lys Leu Glu Gly Gly
385 390 395 400
Cys His His Thr Ala Ala Met His Ser Asn Asn Ile Ser Asn Leu Asn
405 410 415
Arg Met Ala Asn Ala Ile Asp Thr Ser Ile Phe Val Lys Asn Gly Pro
420 425 430
Cys Ile Ala Gly Leu Gly Leu Gly Gly Glu Gly Trp Thr Thr Met Thr
435 440 445
Ile Thr Thr Pro Thr Gly Glu Gly Val Thr Cys Ala Arg Thr Phe Val
450 455 460
Arg Leu Arg Arg Cys Thr Met Val Asp Ser Phe Arg Ile Val
465 470 475
<210> 84
<211> 477
<212> PRT
<213> AADHpa
<400> 84
Met Glu Ile Gln Asn Leu Glu Glu Asp Ile Arg Arg Ile Leu Ser Glu
1 5 10 15
Glu Leu Lys Lys Ser Gly Thr Ser Gln Thr Ala Ser Thr Ser Asp Ala
20 25 30
Gly Gln Asn Gly Ile Phe Lys Thr Val Asp Glu Ala Ile Ala Ala Ala
35 40 45
Lys Ala Ala Glu Asp Val Tyr Ile Asp Lys Thr Leu Ala Phe Arg Glu
50 55 60
Lys Val Leu Thr Ala Ile Arg Glu Gly Phe Arg Pro Tyr Ile Glu Lys
65 70 75 80
Met Ala Lys Asp Ile Lys Asp Glu Thr Gly Met Gly Thr Val Glu Ala
85 90 95
Lys Ile Ala Lys Leu Asn Asn Ala Leu Tyr Asn Thr Pro Gly Thr Glu
100 105 110
Ile Leu Gln Pro Glu Ala Glu Thr Gly Asp Gly Gly Leu Val Met Tyr
115 120 125
Glu Tyr Ala Pro Phe Gly Val Ile Gly Ala Val Gly Pro Ser Thr Asn
130 135 140
Pro Ser Glu Thr Val Ile Ala Asn Ala Ile Met Met Leu Ala Gly Gly
145 150 155 160
Asn Thr Leu Tyr Phe Gly Ala His Pro Gly Ala Lys Lys Ile Thr Arg
165 170 175
Trp Thr Ile Glu Lys Leu Asn Glu Leu Val Tyr Glu Ala Thr Gly Met
180 185 190
Lys Asn Leu Val Val Ser Ile Glu Glu Pro Ser Ile Glu Ser Val Gln
195 200 205
Glu Met Met Gln His Pro Asp Ile Ala Met Leu Ser Ile Thr Gly Gly
210 215 220
Pro Ala Val Val His Gln Ala Leu Val Ser Gly Lys Lys Ala Val Gly
225 230 235 240
Ala Gly Ala Gly Asn Pro Pro Ala Ile Val Asp Ala Thr Ala Asn Val
245 250 255
Ala Leu Ala Ala His Asn Ile Val Asp Ser Ala Ser Phe Asp Asn Asn
260 265 270
Ile Leu Cys Thr Ala Glu Lys Glu Val Val Val Glu Ser Ser Val Lys
275 280 285
Asp Glu Leu Ile Lys Lys Met Gln Glu Glu Gly Ala Phe Leu Val Thr
290 295 300
Asn Ala Ser Asp Ile Asp Lys Leu Ala Glu Met Thr Ile Gly Lys Asn
305 310 315 320
Gly Ala Pro Asp Arg Gln Phe Val Gly Lys Asp Ala Thr Tyr Ile Leu
325 330 335
Asp Lys Ala Gly Ile Ala Tyr Thr Gly Thr Pro Lys Leu Ile Ile Leu
340 345 350
Glu Ala Gln Lys Asp His Pro Leu Val Thr Thr Glu Met Leu Met Pro
355 360 365
Ile Val Pro Val Val Ser Cys Pro Thr Phe Asp Gln Val Leu Ala Thr
370 375 380
Ala Val Glu Val Glu Gln Gly Leu His His Thr Ala Ser Ile His Ser
385 390 395 400
Glu Asn Leu Pro Asn Ile Asn Arg Ala Ala His Arg Met Asn Thr Ser
405 410 415
Ile Phe Val Val Asn Gly Ala Thr Tyr Val Gly Thr Gly Val Gly Ala
420 425 430
Asn Gly Ala His Ala Gly Ala Ser Ala Leu Thr Ile Ala Thr Pro Thr
435 440 445
Gly Glu Gly Thr Ala Thr Ala Lys Thr Phe Thr Arg Arg Arg Arg Leu
450 455 460
Asn Ser Pro Glu Ala Phe Ser Leu Arg Ser Trp Glu Ala
465 470 475
<210> 85
<211> 477
<212> PRT
<213> AADHpb
<400> 85
Met Gln Thr Thr Glu Ala Thr Ile Arg Asn Val Val Gln Glu Val Leu
1 5 10 15
Ala Gln Leu Ala Lys Gln Ser Asn Gly Ser Asn Gly Val Pro Arg Ala
20 25 30
Glu Arg Lys Ala Gly Asp Trp Gly Val Phe Asp Asp Thr Asp Gln Ala
35 40 45
Ile Ala Ala Ala Asn Glu Gly Phe Arg Ala Leu Ser Ser Lys Gly Met
50 55 60
Glu Glu Arg Ala Lys Ile Val Glu Ile Val Lys Arg Ile Cys Ala Asp
65 70 75 80
Gln Ala Glu Glu Leu Gly Arg Leu Glu Phe Gln Glu Thr Lys Val Gly
85 90 95
Arg Leu Asp His Lys Ile Glu Lys Leu Gln Ile Ile Lys Lys Val Pro
100 105 110
Gly Thr Glu Trp Leu Arg Thr Glu Ala Met Ser Gly Asp His Gly Leu
115 120 125
Thr Val Ile Glu Tyr Ala Pro Phe Gly Val Ile Gly Thr Ile Thr Pro
130 135 140
Val Thr His Ser Leu Pro Thr Leu Ala Gly Asn Val Ile Asn Met Val
145 150 155 160
Ala Ala Gly Asn Thr Leu Val Val Asn Pro His Pro Ser Gly Lys Lys
165 170 175
Ile Ala Cys Glu Gly Val Arg Arg Phe Asn Arg Ala Ile Tyr Ala Ala
180 185 190
Thr Gly Leu Glu Asn Val Ile Thr Ile Val Ser Asn Pro Thr Leu Glu
195 200 205
Ser Ala Asp Arg Ile Phe Asn His Arg Asp Val Lys Met Leu Cys Val
210 215 220
Thr Gly Gly Pro Gly Val Ala Arg Ala Ala Met Ala Ser Arg Lys Lys
225 230 235 240
Ala Val Val Ala Gly Pro Gly Asn Pro Pro Val Leu Val Asp Glu Ser
245 250 255
Ala Cys Leu Gln Asn Ala Ala Glu Ser Ile Ile Ala Gly Ala Gly Tyr
260 265 270
Asp Asn Asn Leu Leu Cys Ile Gly Glu Lys Glu Val Phe Val Val Glu
275 280 285
Ser Val Phe Asp Arg Phe Met Gln Glu Met Asp Lys Ala Gly Ala Val
290 295 300
Arg Leu Asn Ala Ser Gln Val Asp Gln Leu Ser Lys Val Ala Phe Thr
305 310 315 320
Pro Pro Glu Asn Glu Lys Asp His Tyr His Val Asn Arg Asp Leu Val
325 330 335
Gly Gln His Pro Tyr Val Leu Ala Lys Gln Ile Gly Leu Pro Leu Pro
340 345 350
Glu Ser Thr Pro Leu Leu Phe Gly Glu Thr Asp Glu His Asn Pro Phe
355 360 365
Val Ala Glu Glu Gln Met Met Pro Phe Val Pro Ile Val Arg Cys Arg
370 375 380
Asn Ala Glu His Gly Met Asp Leu Cys Glu Lys Tyr Glu His Gly Phe
385 390 395 400
Arg His Thr Ser Leu Ile His Ser Arg Asn Val Arg Asn Met Ser Ile
405 410 415
Met Gly Lys Arg Met Asp Thr Thr Leu Phe Ile Lys Asn Gly Pro Ser
420 425 430
Pro Ala Gly Leu Gly Leu Gly Gly Glu Gly Tyr Leu Ser Phe Ser Ile
435 440 445
Ala Thr Pro Thr Gly Glu Gly Val Thr Thr Pro Leu Thr Phe Thr Arg
450 455 460
Gln Arg Arg Cys Thr Met Val Asp Asp Leu Arg Ile Val
465 470 475
<210> 86
<211> 452
<212> PRT
<213> AADHpe
<400> 86
Met Glu Ile Lys Glu Met Val Ala Leu Ala Arg Lys Ala Gln Lys Glu
1 5 10 15
Tyr Gln Ala Thr His Asn Gln Gln Ser Val Asp Asn Ile Cys Arg Ala
20 25 30
Ala Ala Lys Val Ile Tyr Glu Asn Ala Glu Met Leu Ala Arg Glu Ala
35 40 45
Val Asp Glu Thr Gly Met Gly Val Tyr Glu Asp Lys Val Ser Lys Asn
50 55 60
Arg Gly Lys Ser Lys Gly Val Trp Tyr Asn Leu His Asp Lys Lys Ser
65 70 75 80
Val Gly Ile Leu Asn Ile Asp Glu Arg Thr Gly Met Ile Glu Ile Ala
85 90 95
Lys Pro Met Gly Val Val Gly Ala Val Thr Pro Thr Thr Asn Pro Ile
100 105 110
Val Thr Pro Met Ser Asn Ile Ile Phe Ala Leu Lys Thr Cys Asn Ala
115 120 125
Ile Ile Ile Ser Pro His Pro Arg Ser Lys Lys Cys Ser Lys His Ala
130 135 140
Val Asp Leu Ile Leu Glu Ala Ile Lys Pro Phe Asn Val Pro Glu Gly
145 150 155 160
Met Val Gln Val Ile Glu Glu Pro Ser Ile Glu Met Thr Gln Gln Leu
165 170 175
Met Gly Ala Val Asp Val Val Val Ala Thr Gly Gly Met Ala Met Val
180 185 190
Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ser Tyr Gly Val Gly Ala Gly
195 200 205
Asn Val Gln Val Ile Phe Asp Ser Asp Ile Asp Phe Glu Asp Ala Ala
210 215 220
Glu Lys Val Ile Tyr Gly Arg Ser Tyr Asp Asn Gly Ile Ile Cys Thr
225 230 235 240
Gly Glu Gln Ser Val Ile Tyr Asn Glu Ala Asp Lys Asp Ala Val Phe
245 250 255
Ala Ala Leu Arg Lys Arg Gly Ala Tyr Ile Cys Thr Glu Glu Glu Gly
260 265 270
Lys Lys Met Arg Asp Leu Leu Phe Gln Asn Gly Gly Pro Ile Asn Lys
275 280 285
Asp Val Val Gly Gln Gly Val Gln Lys Ile Ala Lys Met Ala Gly Ile
290 295 300
Asn Val Pro Glu Gly Ser Arg Val Ile Ile Val Glu Ala Asn Gly Val
305 310 315 320
Gly Glu Glu Asp Val Ile Cys Arg Glu Lys Met Cys Pro Val Met Cys
325 330 335
Ala Leu Ser Tyr Lys His Phe Glu Glu Gly Val Asp Ile Ala Cys Arg
340 345 350
Asn Leu Ala Asn Glu Gly Asn Gly His Ser Cys Ala Ile His Ser Asn
355 360 365
Asn Gln Ala His Ile Ile Leu Thr Gly Ser Lys Ala Thr Val Ser Arg
370 375 380
Ile Val Val Asn Ala Pro Ser Ala Thr Thr Ala Gly Gly His Ile Gln
385 390 395 400
Asn Gly Leu Asn Val Thr Asn Thr Leu Gly Cys Gly Ser Trp Gly Asn
405 410 415
Asn Ser Ile Ser Glu Asn Phe Thr Tyr Lys His Leu Met Asn Ile Ser
420 425 430
Arg Ile Ala Pro Leu Asn Pro Ala Ile Lys Val Pro Asn Asp Gln Glu
435 440 445
Ile Trp Glu Leu
450
<210> 87
<211> 460
<212> PRT
<213> AADHrw
<400> 87
Met Thr Val Thr Ala Thr Leu Asp Ala Asp Leu Ala Ala Leu Ala Asp
1 5 10 15
Val Arg Ala Gln Val Ser Ala Thr Lys Lys Ala Phe Asp Ala Ile Glu
20 25 30
Gly Ala Asp Gln Gln Trp Leu Asp Glu Ile Val Arg Ala Met Ala Lys
35 40 45
Ala Gly Tyr Asp Ala Ser Glu Glu Leu Ala Arg Met Ala Val Asp Glu
50 55 60
Thr Gly Tyr Gly Val Tyr Glu Asn Lys Ile Ile Lys Asn Arg Phe Asn
65 70 75 80
Thr Gly Phe Val Ala Asp Trp Met Leu Glu Arg Arg Ala Val Gly Val
85 90 95
Leu Trp Ala Asp Glu Glu Asn Lys Val Thr Ala Val Gly Ser Pro Met
100 105 110
Gly Val Ile Ala Ala Ile Ile Pro Val Thr Asn Pro Thr Ala Thr Val
115 120 125
Leu Phe Lys Ser Leu Ala Ser Val Lys Ala Gly Asn Ala Val Val His
130 135 140
Ala Pro His Pro Arg Ala Ala Arg Cys Ser Ala Arg Ala Ala Glu Ile
145 150 155 160
Met Ala Glu Ala Ala Val His Ala Gly Ala Pro Glu Asn Leu Val Ser
165 170 175
Cys Leu Arg Gln Pro Thr Leu Asp Ala Thr Arg Glu Leu Met Arg His
180 185 190
Gly Asp Ile Arg Leu Val Leu Ala Thr Gly Gly Pro Ala Met Val Ala
195 200 205
Ala Ala Tyr Ser Ser Gly Lys Pro Thr Ile Ala Val Gly Ala Gly Asn
210 215 220
Val Pro Ala Tyr Val Asp Ser Thr Val Lys Asp Leu Ala Glu Thr Ala
225 230 235 240
Thr Met Ile Val Thr Ser Lys Ser Phe Asp Asn Gly Thr Ala Cys Val
245 250 255
Ala Glu Gln Ser Val Val Ile Ala Ala Pro Val Ala Asp Gln Leu Val
260 265 270
Gln Glu Leu Gly Arg Ala Gly Ala Thr Trp Met Thr Lys Asp Gln Gln
275 280 285
Ala Ala Leu Thr Arg Thr Leu Phe Asp Glu Arg Gly Gly Leu Arg Ala
290 295 300
Asn Ala Val Gly Gln Ser Ala Val Arg Leu Ala Glu Met Ser Gly Phe
305 310 315 320
Thr Val Pro Ala Ser Thr Lys Val Leu Ala Ala Glu Leu Asp Glu Val
325 330 335
Gly Asp His Val Leu Leu Ser Arg Glu Ile Leu Gly Pro Val Leu Thr
340 345 350
Val Tyr Arg Glu Pro Asp Tyr Gln Ala Gly Trp Arg Arg Cys Arg Glu
355 360 365
Ile Leu Ala Leu Gly Gly Glu Gly His Thr Val Ala Val His Thr Ser
370 375 380
Asp Pro Glu Val Val Ala Lys Phe Gly Glu Leu Pro Ala Gly Arg Ile
385 390 395 400
Val Val Asn Thr Pro Ala Leu Phe Gly Gly Met Gly Tyr Ser Thr Asn
405 410 415
Val Asp Pro Ser Phe Gln Ile Gly Thr Gly Thr Trp Ser Gly Ser Ile
420 425 430
Cys Ser Asp Asn Val Thr Pro Leu His Leu Val Asn Ile Lys Arg Ile
435 440 445
Ala His Glu Ala Arg Pro Trp Arg Gly Val Phe Asp
450 455 460
<210> 88
<211> 871
<212> PRT
<213> AADHsl
<400> 88
Met Ala Ile Glu Glu His Ser Leu Asn Asp Lys Glu Ser Lys Lys Glu
1 5 10 15
Thr Leu Glu Glu Thr Ile Asp Leu Leu Val Thr Asn Ala Lys Thr Ala
20 25 30
Ser Glu Glu Phe Thr Pro Leu Asp Gln Gln Gln Ile Asp Glu Ile Val
35 40 45
Arg Gln Met Ala Leu Ala Gly Leu Asp Gln His Met Gln Leu Ala Lys
50 55 60
Met Ala Val Glu Glu Thr Lys Arg Gly Val Tyr Glu Asp Lys Met Ile
65 70 75 80
Lys Asn Ile Phe Ala Thr Glu Tyr Ile Tyr Asn Ser Ile Lys Lys Asn
85 90 95
Lys Thr Val Gly Val Ile Ser Glu Asp Pro Tyr Glu Glu Ile Val Gln
100 105 110
Ile Ala Glu Pro Ile Gly Val Ile Ala Gly Val Thr Pro Val Thr Asn
115 120 125
Pro Thr Ser Thr Thr Met Phe Lys Ser Leu Ile Ser Ile Lys Thr Lys
130 135 140
Asn Pro Ile Ile Phe Ala Phe His Pro Ser Ala Gln Lys Cys Ser Ala
145 150 155 160
Ala Ala Ala Lys Val Val Arg Asp Ala Ala Val Lys Ala Gly Ala Pro
165 170 175
Lys Asn Cys Ile Gln Trp Ile Glu Thr Pro Ser Leu Glu Ala Thr Gln
180 185 190
Arg Leu Met His His Pro Gly Val Ser Leu Ile Leu Ala Thr Gly Gly
195 200 205
Ser Gly Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro Ala Ile Gly
210 215 220
Val Gly Pro Gly Asn Val Pro Ala Tyr Ile Glu Lys Ser Ala Asp Val
225 230 235 240
Lys Gln Ala Val Asn Asp Leu Ile Leu Ser Lys Thr Phe Asp Asn Gly
245 250 255
Met Ile Cys Ala Ser Glu Gln Ala Val Ile Val Asp Gln Gln Ile Tyr
260 265 270
Glu Ala Val Lys Lys Glu Met Ile Asp Asn Lys Cys Tyr Phe Leu Asn
275 280 285
Asn Glu Glu Lys Lys Lys Val Glu Lys Leu Val Ile Asp Glu Arg Arg
290 295 300
Cys Ala Val Asn Pro Gln Ile Val Gly Lys Thr Ala Ala Phe Ile Ala
305 310 315 320
Gly Leu Ala Gly Val Thr Val Pro Glu Asp Thr Lys Val Ile Val Ala
325 330 335
Glu Leu Ser Ser Val Gly Pro Glu Ser Pro Leu Ser Arg Glu Lys Leu
340 345 350
Ser Pro Val Leu Ala Cys Phe Arg Ala Lys Thr Thr Glu Glu Gly Phe
355 360 365
Lys Arg Cys Glu Glu Met Leu Ala Phe Gly Gly Ile Gly His Ser Ala
370 375 380
Val Ile His Ser Asn Asp Gln Lys Val Ile Asp Ala Phe Ala Met Arg
385 390 395 400
Met Lys Ala Gly Arg Leu Ile Val Asn Ala Pro Ser Ser Leu Gly Gly
405 410 415
Ile Gly Asp Ile Tyr Asn His Phe Ile Pro Ser Leu Thr Leu Gly Cys
420 425 430
Gly Ser Tyr Gly Gly Asn Ser Val Ser Thr Asn Val Gly Thr Ala Asn
435 440 445
Leu Ile Asn Ile Lys Thr Leu Ala Asn Arg Arg Asn Asn Met Gln Trp
450 455 460
Phe Lys Val Pro Pro Lys Ile Tyr Phe Glu Lys Asn Ala Thr Lys Tyr
465 470 475 480
Leu Ser Val Met Pro Asp Ile Thr Arg Ala Phe Ile Val Ala Asp Pro
485 490 495
Gly Met Val Lys Phe Gly Tyr Val Asp Arg Val Ile His Phe Leu Lys
500 505 510
Gln Arg Arg Asp Pro Val His Tyr Glu Val Phe Ser Asp Val Glu Pro
515 520 525
Asp Pro Ser Val Ala Thr Val Met Lys Gly Thr Glu Leu Met Arg Lys
530 535 540
Phe Glu Pro Asp Val Ile Ile Ala Leu Gly Gly Gly Ser Ala Met Asp
545 550 555 560
Ala Ala Lys Gly Met Trp Leu Phe Tyr Glu Ser Pro Glu Thr Asp Phe
565 570 575
Phe Gly Leu Lys Gln Lys Phe Leu Asp Ile Arg Lys Arg Val Phe Lys
580 585 590
Phe Pro Asp Leu Gly Lys Lys Ala Lys Phe Val Ala Ile Pro Thr Thr
595 600 605
Ser Gly Thr Gly Ser Glu Val Thr Ser Phe Ser Val Ile Thr Asp Lys
610 615 620
Glu Asn Asp Met Lys Tyr Pro Leu Ala Asp Tyr Ala Leu Thr Pro Asn
625 630 635 640
Val Ala Ile Ile Asp Pro Glu Phe Val Leu Thr Val Pro Gln Arg Val
645 650 655
Leu Ala Asp Ser Gly Met Asp Val Leu Thr His Ala Ile Glu Ala Tyr
660 665 670
Val Ser Asn Met Ala Asn Asp Phe Thr Asp Gly Leu Cys Ile Lys Ala
675 680 685
Ile Gln Leu Val Phe Lys Tyr Leu Pro Arg Ser Tyr Lys Asp Ser Thr
690 695 700
Asp Arg Glu Ala Lys Glu Lys Met His Asn Ala Ser Thr Ile Ala Gly
705 710 715 720
Met Ala Phe Ser Asn Ala Phe Leu Gly Ile Asn His Ser Leu Ala His
725 730 735
Lys Leu Gly Asn Glu Phe His Leu Ala His Gly Arg Cys Cys Ala Ile
740 745 750
Leu Leu Pro His Val Ile Arg Phe Asn Ala Gln Lys Pro Thr Lys Phe
755 760 765
Ala Ala Phe Pro Lys Tyr Lys His Phe Ile Ala Asp Glu Arg Tyr Ala
770 775 780
Glu Ile Ala Gln Val Leu Gly Ile Ala Gly Lys Thr Thr Glu Glu Ser
785 790 795 800
Val Glu Asn Leu Val Gln Ala Ile Lys Lys Leu Thr Gln Glu Leu Asn
805 810 815
Met Pro Met Ser Ile Glu Lys Asn Gly Val Ser Lys Glu Ala Phe Glu
820 825 830
Ala Ala Val Asp Lys Leu Ala Asp Arg Ala Phe Gly Asp Gln Cys Thr
835 840 845
Pro Ala Asn Pro Lys Met Pro Leu Val Ser Glu Leu Thr Glu Ile Tyr
850 855 860
Arg Gln Ala Phe Lys Gly Ile
865 870
<210> 89
<211> 462
<212> PRT
<213> AADHss
<400> 89
Met Ala Asp Glu Gln Leu Val Gln Asn Val Val Arg Glu Val Val Ala
1 5 10 15
Arg Met Gln Ile Ser Ala Pro Ala Arg Gly Met His Gly Val Phe Ser
20 25 30
Asp Met Glu Glu Ala Ile Glu Ala Ala Arg Thr Ala Gln Gln Thr Val
35 40 45
Arg Leu Leu Pro Met Asp Gln Arg Glu Lys Ile Ile Gly Ala Ile Arg
50 55 60
Arg Lys Thr Arg Glu Asn Ala Glu Ile Leu Ala Arg Met Ala Val Asn
65 70 75 80
Glu Thr Gly Met Gly Asn Val Gly Asp Lys Ile Leu Lys His Leu Leu
85 90 95
Val Ala Asp Lys Val Pro Gly Thr Glu Asp Ile Ser Thr Arg Ala Phe
100 105 110
Ser Gly Asp Arg Gly Leu Thr Leu Ile Glu Met Gly Pro Phe Gly Val
115 120 125
Ile Gly Ala Ile Thr Pro Cys Thr Asn Pro Ser Glu Thr Val Leu Cys
130 135 140
Asn Thr Ile Gly Met Leu Ala Gly Gly Asn Thr Val Val Phe Asn Pro
145 150 155 160
His Pro Gln Ala Ile Lys Thr Thr Leu Phe Thr Ile Gln Met Val Asn
165 170 175
Glu Ala Ser Leu Glu Ala Gly Gly Pro Asp Asn Ile Ala Cys Thr Val
180 185 190
Asp Ala Pro Thr Leu Ala Thr Ser Glu Ile Met Met Lys Ser Pro His
195 200 205
Ile Lys Leu Leu Val Ala Thr Gly Gly Pro Gly Val Val Thr Ala Val
210 215 220
Leu Ser Ser Gly Lys Arg Ala Ile Gly Ala Gly Ala Gly Asn Pro Pro
225 230 235 240
Ala Leu Val Asp Glu Thr Ala Asp Ile Arg Lys Ala Ala Glu Asp Ile
245 250 255
Val Asn Gly Cys Thr Phe Asp Asn Asn Leu Pro Cys Ile Ala Glu Lys
260 265 270
Glu Ile Val Ala Val Asp Ser Ile Ala Asp Glu Leu Leu His Tyr Met
275 280 285
Leu Thr Glu Gln Gly Cys Tyr Gln Ala Ser Glu Glu Glu Leu Asp Arg
290 295 300
Leu Thr Lys Ala Val Met Asp Glu Lys Gly Arg Leu Asn Arg Lys Ala
305 310 315 320
Val Gly Arg Ser Ala Arg Lys Leu Leu Ser Met Ile Gly Val Glu Val
325 330 335
Asp Ala Asn Ile Arg Cys Ile Thr Phe Phe Gly Pro Lys Glu His Pro
340 345 350
Leu Ile Thr Thr Glu Leu Met Met Pro Ile Leu Gly Ile Val Arg Val
355 360 365
Lys Asp Phe Ala Glu Gly Leu Glu Thr Ala Ala Trp Leu Glu His Gly
370 375 380
Asn Lys His Ser Ala His Ile His Ser Lys Asn Val Asp Arg Ile Thr
385 390 395 400
Glu Tyr Ala Arg Arg Leu Asp Thr Thr Ile Thr Val Lys Asn Gly Pro
405 410 415
Ser Tyr Ala Ala Leu Gly Phe Gly Gly Glu Ser Tyr Cys Thr Phe Thr
420 425 430
Ile Ala Ser Arg Thr Gly Glu Gly Leu Thr Ser Ala Arg Ser Phe Ile
435 440 445
Lys Ser Arg His Cys Val Met Thr Asp Ser Leu Cys Val Arg
450 455 460
<210> 90
<211> 467
<212> PRT
<213> AADHta
<400> 90
Met Asn Asn Thr Glu Leu Glu Ser Leu Ile Arg Thr Ile Leu Thr Glu
1 5 10 15
Gln Leu Thr Pro Ser Ala Thr Asp Thr Pro Ala Cys Thr Ala Ser Ser
20 25 30
Val Ala Leu Phe Asp Asp Val Asp Ser Ala Ile Cys Ala Ala His Ala
35 40 45
Ala Phe Leu Arg Tyr Gln Glu Ala Pro Leu Lys Thr Arg Ser Ala Ile
50 55 60
Ile Ala Ala Ile Arg Ala Glu Ile Ala Pro Cys Leu Ser Glu Leu Ala
65 70 75 80
Glu Arg Ala Ala Ala Glu Thr Gly Met Gly Asn Thr Ala Asp Lys Ile
85 90 95
Leu Lys Asn Lys Ala Ala Leu Glu Asn Thr Pro Gly Ile Glu Asp Leu
100 105 110
Lys Thr Thr Ala Leu Thr Gly Asp Glu Gly Met Val Leu Phe Glu Tyr
115 120 125
Ser Pro Phe Gly Val Val Gly Ala Val Ala Pro Ser Thr Asn Pro Thr
130 135 140
Glu Thr Ile Ile Asn Asn Ser Ile Ser Met Leu Ala Ala Gly Asn Ala
145 150 155 160
Ile Tyr Phe Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu Trp Leu
165 170 175
Ile Gln Lys Met Glu Glu Ile Ala Phe Lys Val Cys Gly Ile His Asn
180 185 190
Leu Ile Val Thr Val Lys Glu Pro Thr Phe Glu Ala Thr Gln Gln Met
195 200 205
Met Ala His Asp Lys Ile Ala Leu Leu Ala Ile Thr Gly Gly Pro Gly
210 215 220
Ile Val Asn Met Gly Leu Lys Ser Gly Lys Lys Val Ile Gly Ala Gly
225 230 235 240
Ala Gly Asn Pro Pro Cys Leu Val Asp Glu Thr Ala Glu Ile Val Lys
245 250 255
Ala Ala Gln Asp Ile Val Ala Gly Ala Ser Phe Asp Tyr Asn Leu Pro
260 265 270
Cys Ile Ala Glu Lys Ser Val Ile Ala Val Asp Cys Ile Ala Asp Gln
275 280 285
Leu Ile Gln Gln Met Arg Glu Phe Gly Ala Met Gln Ile Thr Asp Pro
290 295 300
Gln Gln Ile Ala Gln Leu Arg Glu Val Cys Ile Gln Lys Gly Ala Ala
305 310 315 320
Asn Lys Ser Leu Val Gly Lys Ser Pro Ala Thr Ile Leu Ala Ala Ala
325 330 335
Gly Ile Pro Cys Pro Ala Lys Glu Pro Arg Leu Ile Ile Leu Glu Val
340 345 350
Pro Ala Asn Asp Pro Phe Val Val Thr Glu Gln Leu Met Pro Val Leu
355 360 365
Pro Ile Val Arg Val Asp Asn Phe Glu Gln Gly Leu Gln Leu Ala Leu
370 375 380
Lys Val Glu Asp Gly Leu His His Thr Ala Met Met His Ser Gln Asn
385 390 395 400
Val Ser Arg Leu Asn Lys Ala Ala His Leu Met Gln Thr Ser Ile Phe
405 410 415
Val Lys Asn Gly Pro Ser Tyr Ala Gly Ile Gly Val Gly Ala Glu Gly
420 425 430
Phe Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser
435 440 445
Ala Arg Thr Phe Gly Arg Leu Arg Arg Cys Val Leu Thr Asn Gly Phe
450 455 460
Ser Ile Arg
465
<210> 91
<211> 474
<212> PRT
<213> AADHtl
<400> 91
Met Val Asp Ile Asp Leu Lys Asn Val Glu Gln Ile Val Arg Thr Val
1 5 10 15
Leu Ala Ser Met Gln Thr Gln Pro Gln Ala Val Gln Pro Ala Ala Gly
20 25 30
Arg Arg Ala Ala Pro Gly Val Phe Thr Glu Leu Asp Asp Ala Val Ala
35 40 45
Glu Ala Ser Thr Ala Gln Arg Ala Ile Arg Asn Leu Ala Thr Arg Val
50 55 60
Lys Val Val Glu Ala Ile Arg Lys Ala Ala Glu Ala His Ala Arg Glu
65 70 75 80
Leu Ala Glu Leu Ala Val Ala Glu Thr Gly Met Gly Arg Val Glu Asp
85 90 95
Lys Phe Ala Lys Asn Val Ser Gln Ala Arg His Thr Pro Gly Val Glu
100 105 110
Cys Leu Ala Pro Thr Ala Leu Thr Gly Asp Arg Gly Leu Thr Leu Ile
115 120 125
Glu Asn Ala Ala Trp Gly Val Ile Ala Ser Val Thr Pro Ser Thr Asn
130 135 140
Pro Ala Ala Thr Ile Ile Asn Asn Ala Ile Ser Met Ile Ser Ala Gly
145 150 155 160
Asn Thr Val Val Phe Ala Pro His Pro Ala Ala Lys Lys Val Ser Gln
165 170 175
Arg Thr Val Ser Leu Leu Asn Glu Ala Ser Val Ala Ala Gly Gly Pro
180 185 190
Asp Asn Val Val Thr Thr Val Leu Asn Pro Asp Ile Ala Thr Ala Gln
195 200 205
Arg Leu Phe Ser Tyr Pro Gly Ile His Leu Leu Thr Val Thr Gly Gly
210 215 220
Glu Ala Val Val Glu Ala Ala Arg Lys His Thr Asp Lys Arg Leu Ile
225 230 235 240
Ala Ala Gly Ala Gly Asn Pro Pro Val Val Val Asp Glu Thr Ala Asp
245 250 255
Leu Ala Arg Ala Ala Arg Asp Ile Val Trp Gly Ala Ser Phe Asp Asn
260 265 270
Asn Ile Ile Cys Val Asp Glu Lys Glu Ile Ile Val Val Asp Arg Val
275 280 285
Cys Asp Ala Leu Lys Thr Glu Met Ala Arg His Lys Ala Val Glu Leu
290 295 300
Thr Pro Asp Gln Gly Lys Arg Leu Val Asp Val Leu Leu Asn Tyr Val
305 310 315 320
Gly Ala Asp Gly Arg Gly Thr Val Ser Arg Asp Trp Val Gly Arg Asp
325 330 335
Ala Ala Lys Ile Ala Ala Ala Ile Gly Leu Glu Val Pro Ala Asp Thr
340 345 350
Arg Leu Leu Tyr Ile Glu Thr Pro Ala Asp His Pro Phe Ala Val Thr
355 360 365
Glu Leu Met Met Pro Val Ile Pro Val Val Arg Ala Pro Asp Val Asp
370 375 380
Ala Ala Ile Asp Leu Ala Val Lys Leu Glu Gly Asn Cys Arg His Thr
385 390 395 400
Ala Ala Met His Ser Arg Asn Ile Asp Ala Leu Asp Arg Met Ala Asn
405 410 415
Ala Ile Asn Thr Ser Ile Phe Val Lys Asn Gly Pro Cys Leu Ala Gly
420 425 430
Leu Gly Phe Gly Gly Glu Gly Trp Thr Thr Met Thr Ile Ser Thr Pro
435 440 445
Thr Gly Glu Gly Val Thr Ser Ala Arg Thr Phe Val Arg Leu Arg Arg
450 455 460
Cys Val Leu Val Asp His Phe Arg Ile Val
465 470
<210> 92
<211> 912
<212> PRT
<213> AADHtm
<400> 92
Met Asp Thr Ala Val Ala Val Gln Lys Gln Asp Ile Ala Met Ser Ala
1 5 10 15
Ala Ser Lys Pro Lys Ser Ala Ser Gly Pro Pro Phe Asp Val Gln Ala
20 25 30
His Val Asp Gly Leu Val Ala Arg Ala Ile Arg Ala Leu Ala Glu Phe
35 40 45
Gln Ala Leu Asp Gln Glu Gln Val Asp Arg Ile Val Ala Lys Ala Ser
50 55 60
Val Ala Ala Leu Asn Gln His Gly Val Leu Ala Lys Leu Ala Val Glu
65 70 75 80
Glu Thr Gly Arg Gly Leu Phe Glu Asp Lys Ala Val Lys Asn Met Phe
85 90 95
Ala Cys Glu Asn Val Thr Ser Ala Met Lys Arg Met Lys Thr Val Gly
100 105 110
Val Ile His Arg Asp Asn Leu Asn Gly Ile Val Glu Ile Ala Glu Pro
115 120 125
Val Gly Val Val Ala Ala Val Thr Pro Val Thr Asn Pro Thr Ser Thr
130 135 140
Ala Ile Phe Lys Ala Leu Val Cys Leu Lys Thr Arg Asn Pro Ile Val
145 150 155 160
Phe Gly Phe His Pro His Ala Gln Lys Cys Ser Ala Glu Ala Val Arg
165 170 175
Ile Val Arg Asp Ala Ala Val Ala Ala Gly Ala Pro Thr Asn Cys Ala
180 185 190
Gln Trp Ile Glu Val Pro Ser Ile Lys Ala Thr Gly Ala Leu Met Asn
195 200 205
His Pro Asp Ile Ser Val Ile Leu Ala Thr Gly Gly Asp Ala Met Val
210 215 220
Lys Ala Ala Tyr Ser Cys Gly Lys Pro Ala Ile Gly Val Gly Pro Gly
225 230 235 240
Asn Val Pro Ala Phe Val Glu Lys Thr Ala Asp Val Ala Arg Ala Ala
245 250 255
Tyr Asp Leu Val Leu Ser Lys Ser Phe Asp Tyr Gly Met Val Cys Ala
260 265 270
Ser Glu Gln Ala Ala Ile Ile Glu Ala Pro Val Tyr Asp Gln Ala Met
275 280 285
Ala Glu Met Gln Arg Leu His Ala His Val Ala Thr Ser Asp Glu Lys
290 295 300
Arg Lys Leu Glu Glu Leu Ala Phe Gly Val Val Ala Asn Ser Glu Asn
305 310 315 320
Cys Gly Ser Ala Lys Leu Asn Pro Ala Ile Val Gly Lys Ser Ala Ser
325 330 335
Trp Leu Ala Lys Gln Ala Gly Phe Ser Val Pro Gln Asp Thr Ser Ile
340 345 350
Leu Leu Val Glu Ala Asp His Val Gly Tyr Asp Glu Pro Leu Thr Arg
355 360 365
Glu Lys Leu Cys Pro Ile Leu Ala Val Ile Arg Ala Arg Asp Ala Asp
370 375 380
Asp Gly Ile Arg Leu Ser Glu Gln Met Val Glu Gln Asp Gly Leu Gly
385 390 395 400
His Ser Ala Cys Ile His Ser Thr Asp Glu Glu Val Ile Glu Lys Phe
405 410 415
Gly Arg Arg Ile Lys Ala Val Arg Ile Ile Thr Asn Ser Pro Thr Ser
420 425 430
Leu Gly Gly Ile Gly Asp Ile Tyr Asn Ala Phe Val Pro Ser Leu Thr
435 440 445
Leu Gly Cys Gly Thr Tyr Gly Lys Asn Ser Val Ser Asn Asn Val Gln
450 455 460
Ala Val Asn Leu Ile Asn Val Lys Arg Ile Gly His Arg Asn Asn Asn
465 470 475 480
Ile Gln Trp Phe Lys Val Pro Ala Lys Ile Tyr Phe Glu Pro Asn Ala
485 490 495
Ile Arg Tyr Leu Ala Asp Met Arg Arg Val Asn Arg Val Phe Ile Val
500 505 510
Thr Asp Ala Thr Met Ser Lys Leu Gly Val Val Asp Arg Ile Arg Asp
515 520 525
Val Leu Asn Arg Arg Leu Asn Met Val Thr Met Gln Val Val Asp Thr
530 535 540
Ile Asp Ser Glu Pro Ser Ile Ser Met Val Glu Val Gly Ala Glu Gln
545 550 555 560
Met Arg Asn Phe Gln Pro Asp Thr Ile Val Ala Leu Gly Gly Gly Ser
565 570 575
Ala Met Asp Ala Ala Lys Val Met Trp Leu Leu Tyr Glu His Pro Glu
580 585 590
Val Ser Phe Ser Asp Val Arg Glu Lys Cys Phe Asp Val Arg Lys Arg
595 600 605
Ala Phe Thr Phe Pro Asp Leu Gly Glu Lys Ala Gln Leu Val Cys Ile
610 615 620
Ser Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro Phe Ala Cys Ile
625 630 635 640
Thr Asp Pro Lys Thr Gly Tyr Lys Tyr Gln Leu Thr Asp Tyr Ala Leu
645 650 655
Met Pro Thr Val Ala Ile Val Asp Pro Thr Leu Val Asn Ala Met Pro
660 665 670
Ser Arg Leu Ala Ala Asp Ser Gly Met Asp Ala Leu Ala Arg Ala Thr
675 680 685
Glu Ala Tyr Val Ser Val Tyr Ala Asn Asp Phe Thr Asp Gly Leu Ala
690 695 700
Met Gln Ala Ile Lys Leu Ile Phe Glu Asn Leu Pro Ala Ser Val Leu
705 710 715 720
Lys Gly Asp Ala Lys Ala Arg Glu Lys Met His Asn Ala Gly Thr Ile
725 730 735
Ala Gly Met Ala Ser Gly Ser Ala Phe Leu Gly Ile Val His Ala Ile
740 745 750
Thr His Thr Ile Gly Ala Ser Tyr Arg Ile Ser Gln Gly Arg Ser Asn
755 760 765
Ala Thr Leu Leu Pro Leu Gly Ile Arg Tyr Asn Gly Arg Ile Pro Thr
770 775 780
Lys Leu Thr Ser Trp Pro Lys Tyr Glu Lys Tyr Val Ala Pro Gln Arg
785 790 795 800
Tyr Gln Asp Ile Ala Lys Met Leu Gly Leu Pro Ala Ala Thr Pro Glu
805 810 815
Glu Gly Val Glu Ser Tyr Ala Arg Ala Val Glu Asp Leu Arg Ala Thr
820 825 830
Leu Asn Met Glu Tyr Ser Phe Arg Ala Gln Gly Ile Glu Glu His Asp
835 840 845
Phe Val Ala Asn Leu Asp Glu Met Ala Phe Arg Ala Ser Asp Asp Gln
850 855 860
Ser Ala Pro Ala Asn Pro Arg Leu Pro Met Val Glu Asp Leu Lys Ile
865 870 875 880
Ile Met Glu Ala Ala Tyr Tyr Gly Ile Ser Phe Asp Asp Ile Val Ala
885 890 895
Arg Arg Ala Ser Ala Gln Leu Ala Lys Ser Glu Val Ser Lys Lys Gly
900 905 910
<210> 93
<211> 532
<212> PRT
<213> AADHvs
<400> 93
Met Asn Glu Gln Glu Ile Ala His Ala Val Glu Asn Val Leu Ser Lys
1 5 10 15
Tyr Thr Asn Val Thr Ala Gln Asn Ala Glu Pro Val Ser Tyr Ser Ser
20 25 30
Asn Ala Ser Leu Glu Asn Ile Val Ser Gln Ala Leu Ala Gly Asn Met
35 40 45
Val Lys Gln Pro Glu Thr Gln Thr Ala Pro Asp Leu Asn Ser Asn Ile
50 55 60
Glu Asn Ile Val Ser Gln Ile Leu Ala Glu Asn Gln Ala Lys Pro Gln
65 70 75 80
Ser Val Gln Cys Gln Ser Ala Asn His Gly Thr Thr Glu Tyr Leu Gly
85 90 95
Cys Phe Ala Ser Met Glu Glu Ala Ile Ser Ala Ala Ser His Ala Gln
100 105 110
Val Gln Tyr Arg His Cys Thr Met Gly Asp Arg Ala Ser Phe Val Lys
115 120 125
Gly Ile Arg Glu Val Phe Thr Gln Asp Asp Val Leu Glu Lys Ile Ser
130 135 140
Arg Met Ala Val Glu Glu Thr Gly Met Gly Asn Tyr Ala Asp Lys Leu
145 150 155 160
Thr Lys Asn Arg Ile Ala Ala Thr Lys Thr Pro Gly Ile Glu Asp Leu
165 170 175
Thr Thr Ser Ala Leu Ser Gly Asp Ser Gly Leu Thr Leu Thr Glu Phe
180 185 190
Ser Ala Tyr Gly Val Ile Gly Ser Ile Thr Pro Thr Thr Asn Pro Thr
195 200 205
Glu Thr Ile Ile Asn Asn Ser Ile Gly Met Leu Ala Ala Gly Asn Thr
210 215 220
Val Val Tyr Ser Pro His Pro Arg Ser Arg Asn Val Ser Leu Val Ala
225 230 235 240
Val Asp Leu Ile Asn Arg Lys Leu Ala Glu Leu Gly Ala Pro Ala Asn
245 250 255
Leu Val Val Thr Val Leu Glu Pro Ser Ile Asp Asn Thr Asn Ala Met
260 265 270
Met Asn Asp Pro Arg Val Asn Met Leu Val Ala Thr Gly Gly Pro Ser
275 280 285
Ile Val Lys Thr Val Met Ser Thr Gly Lys Lys Ala Ile Gly Ala Gly
290 295 300
Ala Gly Asn Pro Pro Ala Val Val Asp Glu Thr Ala Asn Ile Glu Lys
305 310 315 320
Ala Ala Lys Asp Ile Ile Asn Gly Cys Ala Phe Asp Asn Asn Leu Pro
325 330 335
Cys Ile Ala Glu Lys Glu Val Ile Val Val Asn Glu Val Ala Asp Tyr
340 345 350
Leu Ile His Cys Met Lys Lys Ser Gly Ala Tyr Leu Leu Cys Asp Lys
355 360 365
Gln Lys Ile Gln Gln Leu Gln Ser Leu Val Leu Asn Glu Lys Gly Thr
370 375 380
Gly Pro Asn Thr Ser Phe Val Gly Lys Gly Ala Arg Tyr Ile Leu Asp
385 390 395 400
Lys Leu Asn Ile Gln Val Ser Asp Asp Ile Lys Val Ile Leu Ile Glu
405 410 415
Thr Glu Arg Asn His Pro Phe Val Val His Glu Leu Met Met Pro Ile
420 425 430
Leu Pro Val Val Arg Val Glu Asn Val Asp Glu Ala Ile Asp Leu Ala
435 440 445
Ile Lys Val Glu His Gly Asn Arg His Thr Ala Ile Met His Ser Thr
450 455 460
Asn Val Glu Lys Leu Ser Lys Met Ala Arg Leu Ile Gln Thr Thr Ile
465 470 475 480
Phe Val Lys Asn Gly Pro Ser Tyr Ser Gly Ile Gly Val Gly Gly Glu
485 490 495
Gly His Thr Thr Phe Thr Ile Ala Gly Pro Thr Gly Glu Gly Ile Thr
500 505 510
Ser Ala Arg Ser Phe Ala Arg Tyr Arg Arg Cys Val Met Val Glu Ala
515 520 525
Leu Asn Ile Arg
530
<210> 94
<211> 891
<212> PRT
<213> ADHEec
<400> 94
Met Ala Val Thr Asn Val Ala Glu Leu Asn Ala Leu Val Glu Arg Val
1 5 10 15
Lys Lys Ala Gln Arg Glu Tyr Ala Ser Phe Thr Gln Glu Gln Val Asp
20 25 30
Lys Ile Phe Arg Ala Ala Ala Leu Ala Ala Ala Asp Ala Arg Ile Pro
35 40 45
Leu Ala Lys Met Ala Val Ala Glu Ser Gly Met Gly Ile Val Glu Asp
50 55 60
Lys Val Ile Lys Asn His Phe Ala Ser Glu Tyr Ile Tyr Asn Ala Tyr
65 70 75 80
Lys Asp Glu Lys Thr Cys Gly Val Leu Ser Glu Asp Asp Thr Phe Gly
85 90 95
Thr Ile Thr Ile Ala Glu Pro Ile Gly Ile Ile Cys Gly Ile Val Pro
100 105 110
Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys Ser Leu Ile Ser Leu
115 120 125
Lys Thr Arg Asn Ala Ile Ile Phe Ser Pro His Pro Arg Ala Lys Asp
130 135 140
Ala Thr Asn Lys Ala Ala Asp Ile Val Leu Gln Ala Ala Ile Ala Ala
145 150 155 160
Gly Ala Pro Lys Asp Leu Ile Gly Trp Ile Asp Gln Pro Ser Val Glu
165 170 175
Leu Ser Asn Ala Leu Met His His Pro Asp Ile Asn Leu Ile Leu Ala
180 185 190
Thr Gly Gly Pro Gly Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro
195 200 205
Ala Ile Gly Val Gly Ala Gly Asn Thr Pro Val Val Ile Asp Glu Thr
210 215 220
Ala Asp Ile Lys Arg Ala Val Ala Ser Val Leu Met Ser Lys Thr Phe
225 230 235 240
Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ser Val Val Val Val Asp
245 250 255
Ser Val Tyr Asp Ala Val Arg Glu Arg Phe Ala Thr His Gly Gly Tyr
260 265 270
Leu Leu Gln Gly Lys Glu Leu Lys Ala Val Gln Asp Val Ile Leu Lys
275 280 285
Asn Gly Ala Leu Asn Ala Ala Ile Val Gly Gln Pro Ala Tyr Lys Ile
290 295 300
Ala Glu Leu Ala Gly Phe Ser Val Pro Glu Asn Thr Lys Ile Leu Ile
305 310 315 320
Gly Glu Val Thr Val Val Asp Glu Ser Glu Pro Phe Ala His Glu Lys
325 330 335
Leu Ser Pro Thr Leu Ala Met Tyr Arg Ala Lys Asp Phe Glu Asp Ala
340 345 350
Val Glu Lys Ala Glu Lys Leu Val Ala Met Gly Gly Ile Gly His Thr
355 360 365
Ser Cys Leu Tyr Thr Asp Gln Asp Asn Gln Pro Ala Arg Val Ser Tyr
370 375 380
Phe Gly Gln Lys Met Lys Thr Ala Arg Ile Leu Ile Asn Thr Pro Ala
385 390 395 400
Ser Gln Gly Gly Ile Gly Asp Leu Tyr Asn Phe Lys Leu Ala Pro Ser
405 410 415
Leu Thr Leu Gly Cys Gly Ser Trp Gly Gly Asn Ser Ile Ser Glu Asn
420 425 430
Val Gly Pro Lys His Leu Ile Asn Lys Lys Thr Val Ala Lys Arg Ala
435 440 445
Glu Asn Met Leu Trp His Lys Leu Pro Lys Ser Ile Tyr Phe Arg Arg
450 455 460
Gly Ser Leu Pro Ile Ala Leu Asp Glu Val Ile Thr Asp Gly His Lys
465 470 475 480
Arg Ala Leu Ile Val Thr Asp Arg Phe Leu Phe Asn Asn Gly Tyr Ala
485 490 495
Asp Gln Ile Thr Ser Val Leu Lys Ala Ala Gly Val Glu Thr Glu Val
500 505 510
Phe Phe Glu Val Glu Ala Asp Pro Thr Leu Ser Ile Val Arg Lys Gly
515 520 525
Ala Glu Leu Ala Asn Ser Phe Lys Pro Asp Val Ile Ile Ala Leu Gly
530 535 540
Gly Gly Ser Pro Met Asp Ala Ala Lys Ile Met Trp Val Met Tyr Glu
545 550 555 560
His Pro Glu Thr His Phe Glu Glu Leu Ala Leu Arg Phe Met Asp Ile
565 570 575
Arg Lys Arg Ile Tyr Lys Phe Pro Lys Met Gly Val Lys Ala Lys Met
580 585 590
Ile Ala Val Thr Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro Phe
595 600 605
Ala Val Val Thr Asp Asp Ala Thr Gly Gln Lys Tyr Pro Leu Ala Asp
610 615 620
Tyr Ala Leu Thr Pro Asp Met Ala Ile Val Asp Ala Asn Leu Val Met
625 630 635 640
Asp Met Pro Lys Ser Leu Cys Ala Phe Gly Gly Leu Asp Ala Val Thr
645 650 655
His Ala Met Glu Ala Tyr Val Ser Val Leu Ala Ser Glu Phe Ser Asp
660 665 670
Gly Gln Ala Leu Gln Ala Leu Lys Leu Leu Lys Glu Tyr Leu Pro Ala
675 680 685
Ser Tyr His Glu Gly Ser Lys Asn Pro Val Ala Arg Glu Arg Val His
690 695 700
Ser Ala Ala Thr Ile Ala Gly Ile Ala Phe Ala Asn Ala Phe Leu Gly
705 710 715 720
Val Cys His Ser Met Ala His Lys Leu Gly Ser Gln Phe His Ile Pro
725 730 735
His Gly Leu Ala Asn Ala Leu Leu Ile Cys Asn Val Ile Arg Tyr Asn
740 745 750
Ala Asn Asp Asn Pro Thr Lys Gln Thr Ala Phe Ser Gln Tyr Asp Arg
755 760 765
Pro Gln Ala Arg Arg Arg Tyr Ala Glu Ile Ala Asp His Leu Gly Leu
770 775 780
Ser Ala Pro Gly Asp Arg Thr Ala Ala Lys Ile Glu Lys Leu Leu Ala
785 790 795 800
Trp Leu Glu Thr Leu Lys Ala Glu Leu Gly Ile Pro Lys Ser Ile Arg
805 810 815
Glu Ala Gly Val Gln Glu Ala Asp Phe Leu Ala Asn Val Asp Lys Leu
820 825 830
Ser Glu Asp Ala Phe Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Tyr
835 840 845
Pro Leu Ile Ser Glu Leu Lys Gln Ile Leu Leu Asp Thr Tyr Tyr Gly
850 855 860
Arg Asp Tyr Val Glu Gly Glu Thr Ala Ala Lys Lys Glu Ala Ala Pro
865 870 875 880
Ala Lys Ala Glu Lys Lys Ala Lys Lys Ser Ala
885 890
<210> 95
<211> 885
<212> PRT
<213> ADHEpm
<400> 95
Met Ser Gly Leu Gln Met Phe Gln Asn Leu Ser Leu Tyr Gly Ser Leu
1 5 10 15
Ala Glu Ile Asp Thr Ser Glu Lys Leu Asn Glu Ala Met Asp Lys Leu
20 25 30
Thr Ala Ala Gln Glu Gln Phe Arg Glu Tyr Asn Gln Glu Gln Val Asp
35 40 45
Lys Ile Phe Lys Ala Val Ala Leu Ala Ala Ser Gln Asn Arg Val Ala
50 55 60
Phe Ala Lys Tyr Ala His Glu Glu Thr Gln Lys Gly Val Phe Glu Asp
65 70 75 80
Lys Val Ile Lys Asn Glu Phe Ala Ala Asp Tyr Ile Tyr His Lys Tyr
85 90 95
Cys Asn Asp Lys Thr Ala Gly Ile Ile Glu Tyr Asp Glu Ala Asn Gly
100 105 110
Leu Met Glu Ile Ala Glu Pro Val Gly Pro Val Val Gly Ile Ala Pro
115 120 125
Val Thr Asn Pro Thr Ser Thr Ile Ile Tyr Lys Ser Leu Ile Ala Leu
130 135 140
Lys Thr Arg Asn Cys Ile Ile Phe Ser Pro His Pro Gly Ala His Lys
145 150 155 160
Ala Ser Val Phe Val Val Lys Val Leu His Gln Ala Ala Val Lys Ala
165 170 175
Gly Ala Pro Glu Asn Cys Ile Gln Ile Ile Phe Pro Lys Met Asp Leu
180 185 190
Thr Thr Glu Leu Leu His His Gln Lys Thr Arg Phe Ile Trp Ala Thr
195 200 205
Gly Gly Pro Gly Leu Val His Ala Ser Tyr Thr Ser Gly Lys Pro Ala
210 215 220
Leu Gly Gly Gly Pro Gly Asn Ala Pro Ala Leu Ile Asp Glu Thr Cys
225 230 235 240
Asp Met Asn Glu Ala Val Gly Ser Ile Val Val Ser Lys Thr Phe Asp
245 250 255
Cys Gly Met Ile Cys Ala Thr Glu Asn Ala Val Val Val Val Glu Ser
260 265 270
Val Tyr Glu Asn Phe Val Ala Thr Met Lys Lys Arg Gly Ala Tyr Phe
275 280 285
Met Thr Pro Glu Glu Thr Lys Lys Ala Ser Asn Leu Leu Phe Gly Glu
290 295 300
Gly Met Arg Leu Asn Ala Lys Ala Val Gly Gln Thr Ala Lys Thr Leu
305 310 315 320
Ala Glu Met Ala Gly Phe Glu Val Pro Glu Asn Thr Val Val Leu Cys
325 330 335
Gly Glu Ala Ser Glu Val Lys Phe Glu Glu Pro Met Ala His Glu Lys
340 345 350
Leu Thr Thr Ile Leu Gly Ile Tyr Lys Ala Lys Asp Phe Asp Asp Gly
355 360 365
Val Arg Leu Cys Lys Glu Leu Val Thr Phe Gly Gly Lys Gly His Thr
370 375 380
Ala Val Leu Tyr Thr Asn Gln Asn Asn Lys Asp Arg Ile Glu Lys Tyr
385 390 395 400
Gln Asn Glu Val Pro Ala Phe His Ile Leu Val Asp Met Pro Ser Ser
405 410 415
Leu Gly Cys Ile Gly Asp Met Tyr Asn Phe Arg Leu Ala Pro Ala Leu
420 425 430
Thr Ile Thr Cys Gly Thr Met Gly Gly Gly Ser Ser Ser Asp Asn Ile
435 440 445
Gly Pro Lys His Leu Leu Asn Ile Lys Arg Val Gly Met Arg Arg Glu
450 455 460
Asn Met Leu Trp Phe Lys Ile Pro Lys Ser Val Tyr Phe Lys Arg Ala
465 470 475 480
Ile Leu Ser Glu Ala Leu Ser Asp Leu Arg Asp Thr His Lys Arg Ala
485 490 495
Ile Ile Ile Thr Asp Arg Thr Met Thr Met Leu Gly Gln Thr Asp Lys
500 505 510
Ile Ile Lys Ala Cys Glu Gly His Gly Met Val Cys Thr Val Tyr Asp
515 520 525
Lys Val Val Pro Asp Pro Thr Ile Lys Cys Ile Met Glu Gly Val Asn
530 535 540
Glu Met Asn Val Phe Lys Pro Asp Leu Ala Ile Ala Leu Gly Gly Gly
545 550 555 560
Ser Ala Met Asp Ala Ala Lys Met Met Arg Leu Phe Tyr Glu Tyr Pro
565 570 575
Asp Gln Asp Leu Gln Asp Ile Ala Thr Arg Phe Val Asp Ile Arg Lys
580 585 590
Arg Val Val Gly Cys Pro Lys Leu Gly Arg Leu Ile Lys Thr Leu Val
595 600 605
Cys Ile Pro Thr Thr Ser Gly Thr Gly Ala Glu Val Thr Pro Phe Ala
610 615 620
Val Val Thr Ser Glu Glu Gly Arg Lys Tyr Pro Leu Val Asp Tyr Glu
625 630 635 640
Leu Thr Pro Asp Met Ala Ile Val Asp Pro Glu Phe Ala Val Gly Met
645 650 655
Pro Lys Arg Leu Thr Ser Trp Thr Gly Ile Asp Ala Leu Thr His Ala
660 665 670
Ile Glu Ser Tyr Val Ser Ile Met Ala Thr Asp Phe Thr Arg Pro Tyr
675 680 685
Ser Leu Arg Ala Val Gly Leu Ile Phe Glu Ser Leu Ser Leu Ala Tyr
690 695 700
Asn Asn Gly Lys Asp Ile Glu Ala Arg Glu Lys Met His Asn Ala Ser
705 710 715 720
Ala Ile Ala Gly Met Ala Phe Ala Asn Ala Phe Leu Gly Cys Cys His
725 730 735
Ser Val Ala His Gln Leu Gly Ser Val Tyr His Ile Pro His Gly Leu
740 745 750
Ala Asn Ala Leu Met Leu Ser His Ile Ile Lys Tyr Asn Ala Thr Asp
755 760 765
Ser Pro Val Lys Met Gly Thr Phe Pro Gln Tyr Lys Tyr Pro Gln Ala
770 775 780
Met Arg His Tyr Ala Glu Ile Ala Glu Leu Leu Leu Pro Pro Thr Gln
785 790 795 800
Val Val Lys Met Thr Asp Val Asp Lys Val Gln Tyr Leu Ile Asp Arg
805 810 815
Val Glu Gln Leu Lys Ala Asp Val Gly Ile Pro Lys Ser Ile Lys Glu
820 825 830
Thr Gly Met Val Thr Glu Glu Asp Phe Phe Asn Lys Val Asp Gln Val
835 840 845
Ala Ile Met Ala Phe Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Tyr
850 855 860
Pro Leu Val Ser Glu Leu Lys Gln Leu Met Ile Asp Ala Trp Asn Gly
865 870 875 880
Val Val Pro Lys Leu
885
<210> 96
<211> 459
<212> PRT
<213> EUTEdz
<400> 96
Met Glu His Ser Val Ile Glu Pro Thr Val Pro Met Pro Leu Pro Ala
1 5 10 15
Met Phe Asp Ala Pro Ser Gly Ile Phe Ser Ser Leu Asp Asp Ala Val
20 25 30
Gln Ala Ala Thr Leu Ala Gln Gln Gln Leu Ser Ser Val Glu Leu Arg
35 40 45
Gln Gln Val Ile Lys Ala Ile Arg Val Ala Gly Glu Arg Tyr Ala Gln
50 55 60
Val Leu Ala Glu Met Ala Val Ala Glu Thr Gly Met Gly Arg Val Val
65 70 75 80
Asp Lys Tyr Ile Lys Asn Val Ser Gln Ala Arg His Thr Pro Gly Ile
85 90 95
Glu Cys Leu Ser Ala Glu Val Leu Thr Gly Asp Asn Gly Leu Thr Leu
100 105 110
Ile Glu Asn Ala Pro Trp Gly Val Val Ala Ser Val Thr Pro Ser Thr
115 120 125
Asn Pro Ala Ala Thr Val Ile Asn Asn Ala Ile Ser Met Ile Ala Ala
130 135 140
Gly Asn Ser Val Val Phe Ala Pro His Pro Ser Ala Lys Asn Val Ser
145 150 155 160
Leu Arg Thr Ile Ser Leu Leu Asn Lys Ala Ile Val Ala Thr Gly Gly
165 170 175
Pro Glu Asn Leu Leu Val Ser Val Ala Asn Pro Asn Ile Glu Thr Ala
180 185 190
Gln Arg Leu Phe Arg Tyr Pro Gly Ile Gly Leu Leu Val Val Thr Gly
195 200 205
Gly Glu Ala Val Val Glu Ala Ala Arg Lys His Thr Asp Lys Arg Leu
210 215 220
Ile Ala Ala Gly Ala Gly Asn Pro Pro Val Val Val Asp Glu Thr Ala
225 230 235 240
Asp Ile Pro Lys Ala Ala Arg Ala Ile Val Lys Gly Ala Ser Phe Asp
245 250 255
Asn Asn Ile Ile Cys Ala Asp Glu Lys Val Leu Ile Val Val Asp Arg
260 265 270
Val Ala Asp Ala Leu Leu Ala Glu Met Gln Arg Asn Asn Ala Val Leu
275 280 285
Leu Thr Pro Glu Gln Thr Glu Arg Leu Leu Pro Ala Leu Leu Ser Asp
290 295 300
Ile Asp Glu Gln Gly Lys Gly Arg Val Asn Arg Asp Tyr Val Gly Arg
305 310 315 320
Asp Ala Ala Lys Leu Ala Ala Ala Ile Gly Leu Glu Val Ser Glu His
325 330 335
Thr Arg Leu Leu Leu Ala Glu Thr Asp Ala Asp His Pro Phe Ala Val
340 345 350
Thr Glu Leu Met Met Pro Val Leu Pro Val Ile Arg Val Lys Asn Val
355 360 365
Asp Asp Ala Ile Ala Leu Ala Val Lys Leu Glu Ser Gly Cys Arg His
370 375 380
Thr Ala Ala Met His Ser Thr Asn Ile Arg Asn Leu Asn Arg Met Ala
385 390 395 400
Asn Ala Ile Asn Thr Ser Ile Phe Val Lys Asn Gly Pro Cys Ile Ala
405 410 415
Gly Leu Gly Leu Gly Gly Glu Gly Trp Thr Ser Met Thr Ile Ser Thr
420 425 430
Pro Thr Gly Glu Gly Val Thr Ser Ala Arg Thr Phe Val Arg Leu Arg
435 440 445
Arg Cys Val Leu Val Asp Met Phe Arg Ile Ala
450 455
<210> 97
<211> 467
<212> PRT
<213> EUTEec
<400> 97
Met Asn Gln Gln Asp Ile Glu Gln Val Val Lys Ala Val Leu Leu Lys
1 5 10 15
Met Gln Ser Ser Asp Thr Pro Pro Ala Ala Val His Glu Met Gly Val
20 25 30
Phe Ala Ser Leu Asp Asp Ala Val Ala Ala Ala Lys Val Ala Gln Gln
35 40 45
Gly Leu Lys Ser Val Ala Met Arg Gln Leu Ala Ile Ala Ala Ile Arg
50 55 60
Glu Ala Gly Glu Lys His Ala Arg Asp Leu Ala Glu Leu Ala Val Ser
65 70 75 80
Glu Thr Gly Met Gly Arg Val Glu Asp Lys Phe Ala Lys Asn Val Ala
85 90 95
Gln Ala Arg Gly Thr Pro Gly Val Glu Cys Leu Ser Pro Gln Val Leu
100 105 110
Thr Gly Asp Asn Gly Leu Thr Leu Ile Glu Asn Ala Pro Trp Gly Val
115 120 125
Val Ala Ser Val Thr Pro Ser Thr Asn Pro Ala Ala Thr Leu Ile Asn
130 135 140
Asn Ala Ile Ser Leu Ile Ala Ala Gly Asn Ser Val Ile Phe Ala Pro
145 150 155 160
His Pro Ala Ala Lys Lys Val Ser Gln Arg Ala Ile Thr Leu Leu Asn
165 170 175
Gln Ala Ile Val Ala Ala Gly Gly Pro Glu Asn Leu Leu Val Thr Val
180 185 190
Ala Asn Pro Asp Ile Glu Thr Ala Gln Arg Leu Phe Lys Phe Pro Gly
195 200 205
Ile Gly Leu Leu Val Val Thr Gly Gly Glu Ala Val Val Glu Ala Ala
210 215 220
Arg Lys His Thr Asn Lys Arg Leu Ile Ala Ala Gly Ala Gly Asn Pro
225 230 235 240
Pro Val Val Val Asp Glu Thr Ala Asp Leu Ala Arg Ala Ala Gln Ser
245 250 255
Ile Val Lys Gly Ala Ser Phe Asp Asn Asn Ile Ile Cys Ala Asp Glu
260 265 270
Lys Val Leu Ile Val Val Asp Ser Val Ala Asp Glu Leu Met Arg Leu
275 280 285
Met Glu Gly Gln His Ala Val Lys Leu Thr Ala Glu Gln Ala Gln Gln
290 295 300
Leu Gln Pro Val Leu Leu Lys Asn Ile Asp Glu Arg Gly Lys Gly Thr
305 310 315 320
Val Ser Arg Asp Trp Val Gly Arg Asp Ala Ala Lys Ile Ala Ala Ala
325 330 335
Ile Gly Leu Asn Val Pro Gln Glu Thr Arg Leu Leu Phe Val Glu Thr
340 345 350
Thr Ala Glu His Pro Phe Ala Val Thr Glu Leu Met Met Pro Val Leu
355 360 365
Pro Val Val Arg Val Ala Asn Val Ala Asp Ala Ile Ala Leu Ala Val
370 375 380
Lys Leu Glu Gly Gly Cys His His Thr Ala Ala Met His Ser Arg Asn
385 390 395 400
Ile Glu Asn Met Asn Gln Met Ala Asn Ala Ile Asp Thr Ser Ile Phe
405 410 415
Val Lys Asn Gly Pro Cys Ile Ala Gly Leu Gly Leu Gly Gly Glu Gly
420 425 430
Trp Thr Thr Met Thr Ile Thr Thr Pro Thr Gly Glu Gly Val Thr Ser
435 440 445
Ala Arg Thr Phe Val Arg Leu Arg Arg Cys Val Leu Val Asp Ala Phe
450 455 460
Arg Ile Val
465
<210> 98
<211> 469
<212> PRT
<213> LIN1129li
<400> 98
Met Glu Ser Leu Glu Leu Glu Gln Leu Val Lys Lys Val Leu Leu Glu
1 5 10 15
Lys Leu Ala Glu Gln Lys Glu Val Pro Thr Lys Thr Thr Thr Gln Gly
20 25 30
Ala Lys Ser Gly Val Phe Asp Thr Val Asp Glu Ala Val Gln Ala Ala
35 40 45
Val Ile Ala Gln Asn Cys Tyr Lys Glu Lys Ser Leu Glu Glu Arg Arg
50 55 60
Asn Val Val Lys Ala Ile Arg Glu Ala Leu Tyr Pro Glu Ile Glu Thr
65 70 75 80
Ile Ala Thr Arg Ala Val Ala Glu Thr Gly Met Gly Asn Val Thr Asp
85 90 95
Lys Ile Leu Lys Asn Thr Leu Ala Ile Glu Lys Thr Pro Gly Val Glu
100 105 110
Asp Leu Tyr Thr Glu Val Ala Thr Gly Asp Asn Gly Met Thr Leu Tyr
115 120 125
Glu Leu Ser Pro Tyr Gly Val Ile Gly Ala Val Ala Pro Ser Thr Asn
130 135 140
Pro Thr Glu Thr Leu Ile Cys Asn Ser Ile Gly Met Leu Ala Ala Gly
145 150 155 160
Asn Ala Val Phe Tyr Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu
165 170 175
Trp Leu Ile Glu Lys Leu Asn Thr Ile Val Arg Asp Ser Cys Gly Ile
180 185 190
Asp Asn Leu Ile Val Thr Val Ala Lys Pro Ser Ile Gln Ala Ala Gln
195 200 205
Glu Met Met Asn His Pro Lys Val Pro Leu Leu Val Ile Thr Gly Gly
210 215 220
Pro Gly Val Val Leu Gln Ala Met Gln Ser Gly Lys Lys Val Ile Gly
225 230 235 240
Ala Gly Ala Gly Asn Pro Pro Ser Ile Val Asp Glu Thr Ala Asn Ile
245 250 255
Glu Lys Ala Ala Ala Asp Ile Val Asp Gly Ala Ser Phe Asp His Asn
260 265 270
Ile Leu Cys Ile Ala Glu Lys Ser Val Val Ala Val Asp Ser Ile Ala
275 280 285
Asp Phe Leu Leu Phe Gln Met Glu Lys Asn Gly Ala Leu His Val Thr
290 295 300
Asn Pro Ser Asp Ile Gln Lys Leu Glu Lys Val Ala Val Thr Asp Lys
305 310 315 320
Gly Val Thr Asn Lys Lys Leu Val Gly Lys Ser Ala Thr Glu Ile Leu
325 330 335
Lys Glu Ala Gly Ile Ala Cys Asp Phe Thr Pro Arg Leu Ile Ile Val
340 345 350
Glu Thr Glu Lys Ser His Pro Phe Ala Thr Val Glu Leu Leu Met Pro
355 360 365
Ile Val Pro Val Val Arg Val Pro Asp Phe Asp Glu Ala Leu Glu Val
370 375 380
Ala Ile Glu Leu Glu Gln Gly Leu His His Thr Ala Thr Met His Ser
385 390 395 400
Gln Asn Ile Ser Arg Leu Asn Lys Ala Ala Arg Asp Met Gln Thr Ser
405 410 415
Ile Phe Val Lys Asn Gly Pro Ser Phe Ala Gly Leu Gly Phe Arg Gly
420 425 430
Glu Gly Ser Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr
435 440 445
Thr Thr Ala Arg His Phe Ala Arg Arg Arg Arg Cys Val Leu Thr Asp
450 455 460
Gly Phe Ser Ile Arg
465
<210> 99
<211> 2233
<212> PRT
<213> ACC1sc_S659A
<400> 99
Met Ser Glu Glu Ser Leu Phe Glu Ser Ser Pro Gln Lys Met Glu Tyr
1 5 10 15
Glu Ile Thr Asn Tyr Ser Glu Arg His Thr Glu Leu Pro Gly His Phe
20 25 30
Ile Gly Leu Asn Thr Val Asp Lys Leu Glu Glu Ser Pro Leu Arg Asp
35 40 45
Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile
50 55 60
Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys
65 70 75 80
Trp Ala Tyr Glu Thr Phe Gly Asp Asp Arg Thr Val Gln Phe Val Ala
85 90 95
Met Ala Thr Pro Glu Asp Leu Glu Ala Asn Ala Glu Tyr Ile Arg Met
100 105 110
Ala Asp Gln Tyr Ile Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr
115 120 125
Ala Asn Val Asp Leu Ile Val Asp Ile Ala Glu Arg Ala Asp Val Asp
130 135 140
Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro Leu Leu Pro
145 150 155 160
Glu Lys Leu Ser Gln Ser Lys Arg Lys Val Ile Phe Ile Gly Pro Pro
165 170 175
Gly Asn Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val
180 185 190
Ala Gln Ser Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val
195 200 205
Asp Thr Val His Val Asp Glu Lys Thr Gly Leu Val Ser Val Asp Asp
210 215 220
Asp Ile Tyr Gln Lys Gly Cys Cys Thr Ser Pro Glu Asp Gly Leu Gln
225 230 235 240
Lys Ala Lys Arg Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly
245 250 255
Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile
260 265 270
Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe
275 280 285
Ile Met Lys Leu Ala Gly Arg Ala Arg His Leu Glu Val Gln Leu Leu
290 295 300
Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser
305 310 315 320
Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile
325 330 335
Ala Lys Ala Glu Thr Phe His Glu Met Glu Lys Ala Ala Val Arg Leu
340 345 350
Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr
355 360 365
Ser His Asp Asp Gly Lys Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu
370 375 380
Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro
385 390 395 400
Ala Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser
405 410 415
Asp Ile Arg Thr Leu Tyr Gly Met Asn Pro His Ser Ala Ser Glu Ile
420 425 430
Asp Phe Glu Phe Lys Thr Gln Asp Ala Thr Lys Lys Gln Arg Arg Pro
435 440 445
Ile Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro
450 455 460
Asn Asp Gly Phe Lys Pro Ser Gly Gly Thr Leu His Glu Leu Asn Phe
465 470 475 480
Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly
485 490 495
Asn Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe
500 505 510
Gly Glu Asn Arg Gln Ala Ser Arg Lys His Met Val Val Ala Leu Lys
515 520 525
Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile
530 535 540
Lys Leu Leu Glu Thr Glu Asp Phe Glu Asp Asn Thr Ile Thr Thr Gly
545 550 555 560
Trp Leu Asp Asp Leu Ile Thr His Lys Met Thr Ala Glu Lys Pro Asp
565 570 575
Pro Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Leu Ala
580 585 590
Ser Glu Glu Ala Arg His Lys Tyr Ile Glu Ser Leu Gln Lys Gly Gln
595 600 605
Val Leu Ser Lys Asp Leu Leu Gln Thr Met Phe Pro Val Asp Phe Ile
610 615 620
His Glu Gly Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Gly Asn Asp
625 630 635 640
Arg Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Asp Ile Ile Leu Arg
645 650 655
Gln Leu Ala Asp Gly Gly Leu Leu Ile Ala Ile Gly Gly Lys Ser His
660 665 670
Thr Ile Tyr Trp Lys Glu Glu Val Ala Ala Thr Arg Leu Ser Val Asp
675 680 685
Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg
690 695 700
Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Glu
705 710 715 720
His Ile Ile Lys Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met
725 730 735
Gln Met Pro Leu Val Ser Gln Glu Asn Gly Ile Val Gln Leu Leu Lys
740 745 750
Gln Pro Gly Ser Thr Ile Val Ala Gly Asp Ile Met Ala Ile Met Thr
755 760 765
Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met
770 775 780
Leu Pro Asp Phe Gly Ser Pro Val Ile Glu Gly Thr Lys Pro Ala Tyr
785 790 795 800
Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr
805 810 815
Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val
820 825 830
Leu Arg Asn Pro Lys Leu Pro Tyr Ser Glu Trp Lys Leu His Ile Ser
835 840 845
Ala Leu His Ser Arg Leu Pro Ala Lys Leu Asp Glu Gln Met Glu Glu
850 855 860
Leu Val Ala Arg Ser Leu Arg Arg Gly Ala Val Phe Pro Ala Arg Gln
865 870 875 880
Leu Ser Lys Leu Ile Asp Met Ala Val Lys Asn Pro Glu Tyr Asn Pro
885 890 895
Asp Lys Leu Leu Gly Ala Val Val Glu Pro Leu Ala Asp Ile Ala His
900 905 910
Lys Tyr Ser Asn Gly Leu Glu Ala His Glu His Ser Ile Phe Val His
915 920 925
Phe Leu Glu Glu Tyr Tyr Glu Val Glu Lys Leu Phe Asn Gly Pro Asn
930 935 940
Val Arg Glu Glu Asn Ile Ile Leu Lys Leu Arg Asp Glu Asn Pro Lys
945 950 955 960
Asp Leu Asp Lys Val Ala Leu Thr Val Leu Ser His Ser Lys Val Ser
965 970 975
Ala Lys Asn Asn Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Leu
980 985 990
Cys Lys Leu Ser Ser Lys Val Ser Ala Ile Phe Ser Thr Pro Leu Gln
995 1000 1005
His Ile Val Glu Leu Glu Ser Lys Ala Thr Ala Lys Val Ala Leu
1010 1015 1020
Gln Ala Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys
1025 1030 1035
Glu Arg Thr Glu Gln Ile Glu His Ile Leu Lys Ser Ser Val Val
1040 1045 1050
Lys Val Ala Tyr Gly Ser Ser Asn Pro Lys Arg Ser Glu Pro Asp
1055 1060 1065
Leu Asn Ile Leu Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe
1070 1075 1080
Asp Val Leu Leu Gln Phe Leu Thr His Gln Asp Pro Val Val Thr
1085 1090 1095
Ala Ala Ala Ala Gln Val Tyr Ile Arg Arg Ala Tyr Arg Ala Tyr
1100 1105 1110
Thr Ile Gly Asp Ile Arg Val His Glu Gly Val Thr Val Pro Ile
1115 1120 1125
Val Glu Trp Lys Phe Gln Leu Pro Ser Ala Ala Phe Ser Thr Phe
1130 1135 1140
Pro Thr Val Lys Ser Lys Met Gly Met Asn Arg Ala Val Ser Val
1145 1150 1155
Ser Asp Leu Ser Tyr Val Ala Asn Ser Gln Ser Ser Pro Leu Arg
1160 1165 1170
Glu Gly Ile Leu Met Ala Val Asp His Leu Asp Asp Val Asp Glu
1175 1180 1185
Ile Leu Ser Gln Ser Leu Glu Val Ile Pro Arg His Gln Ser Ser
1190 1195 1200
Ser Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ala Ser Leu
1205 1210 1215
Ser Asn Val Ala Asn Val Cys Val Ala Ser Thr Glu Gly Phe Glu
1220 1225 1230
Ser Glu Glu Glu Ile Leu Val Arg Leu Arg Glu Ile Leu Asp Leu
1235 1240 1245
Asn Lys Gln Glu Leu Ile Asn Ala Ser Ile Arg Arg Ile Thr Phe
1250 1255 1260
Met Phe Gly Phe Lys Asp Gly Ser Tyr Pro Lys Tyr Tyr Thr Phe
1265 1270 1275
Asn Gly Pro Asn Tyr Asn Glu Asn Glu Thr Ile Arg His Ile Glu
1280 1285 1290
Pro Ala Leu Ala Phe Gln Leu Glu Leu Gly Arg Leu Ser Asn Phe
1295 1300 1305
Asn Ile Lys Pro Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr
1310 1315 1320
Glu Ala Val Ser Lys Thr Ser Pro Leu Asp Lys Arg Phe Phe Thr
1325 1330 1335
Arg Gly Ile Ile Arg Thr Gly His Ile Arg Asp Asp Ile Ser Ile
1340 1345 1350
Gln Glu Tyr Leu Thr Ser Glu Ala Asn Arg Leu Met Ser Asp Ile
1355 1360 1365
Leu Asp Asn Leu Glu Val Thr Asp Thr Ser Asn Ser Asp Leu Asn
1370 1375 1380
His Ile Phe Ile Asn Phe Ile Ala Val Phe Asp Ile Ser Pro Glu
1385 1390 1395
Asp Val Glu Ala Ala Phe Gly Gly Phe Leu Glu Arg Phe Gly Lys
1400 1405 1410
Arg Leu Leu Arg Leu Arg Val Ser Ser Ala Glu Ile Arg Ile Ile
1415 1420 1425
Ile Lys Asp Pro Gln Thr Gly Ala Pro Val Pro Leu Arg Ala Leu
1430 1435 1440
Ile Asn Asn Val Ser Gly Tyr Val Ile Lys Thr Glu Met Tyr Thr
1445 1450 1455
Glu Val Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu Gly
1460 1465 1470
Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr Pro
1475 1480 1485
Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met
1490 1495 1500
Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg Gln Ala
1505 1510 1515
Ser Ser Ser Gln Trp Lys Asn Phe Ser Ala Asp Val Lys Leu Thr
1520 1525 1530
Asp Asp Phe Phe Ile Ser Asn Glu Leu Ile Glu Asp Glu Asn Gly
1535 1540 1545
Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala Asn Ala Ile Gly
1550 1555 1560
Met Val Ala Phe Lys Ile Thr Val Lys Thr Pro Glu Tyr Pro Arg
1565 1570 1575
Gly Arg Gln Phe Val Val Val Ala Asn Asp Ile Thr Phe Lys Ile
1580 1585 1590
Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val Thr
1595 1600 1605
Glu Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala
1610 1615 1620
Asn Ser Gly Ala Arg Ile Gly Met Ala Glu Glu Ile Val Pro Leu
1625 1630 1635
Phe Gln Val Ala Trp Asn Asp Ala Ala Asn Pro Asp Lys Gly Phe
1640 1645 1650
Gln Tyr Leu Tyr Leu Thr Ser Glu Gly Met Glu Thr Leu Lys Lys
1655 1660 1665
Phe Asp Lys Glu Asn Ser Val Leu Thr Glu Arg Thr Val Ile Asn
1670 1675 1680
Gly Glu Glu Arg Phe Val Ile Lys Thr Ile Ile Gly Ser Glu Asp
1685 1690 1695
Gly Leu Gly Val Glu Cys Leu Arg Gly Ser Gly Leu Ile Ala Gly
1700 1705 1710
Ala Thr Ser Arg Ala Tyr His Asp Ile Phe Thr Ile Thr Leu Val
1715 1720 1725
Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val Arg Leu Gly
1730 1735 1740
Gln Arg Ala Ile Gln Val Glu Gly Gln Pro Ile Ile Leu Thr Gly
1745 1750 1755
Ala Pro Ala Ile Asn Lys Met Leu Gly Arg Glu Val Tyr Thr Ser
1760 1765 1770
Asn Leu Gln Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val
1775 1780 1785
Ser His Leu Thr Ala Val Asp Asp Leu Ala Gly Val Glu Lys Ile
1790 1795 1800
Val Glu Trp Met Ser Tyr Val Pro Ala Lys Arg Asn Met Pro Val
1805 1810 1815
Pro Ile Leu Glu Thr Lys Asp Thr Trp Asp Arg Pro Val Asp Phe
1820 1825 1830
Thr Pro Thr Asn Asp Glu Thr Tyr Asp Val Arg Trp Met Ile Glu
1835 1840 1845
Gly Arg Glu Thr Glu Ser Gly Phe Glu Tyr Gly Leu Phe Asp Lys
1850 1855 1860
Gly Ser Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val
1865 1870 1875
Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Leu Gly Val Ile Gly
1880 1885 1890
Val Glu Thr Arg Thr Val Glu Asn Leu Ile Pro Ala Asp Pro Ala
1895 1900 1905
Asn Pro Asn Ser Ala Glu Thr Leu Ile Gln Glu Pro Gly Gln Val
1910 1915 1920
Trp His Pro Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile Asn Asp
1925 1930 1935
Phe Asn Asn Gly Glu Gln Leu Pro Met Met Ile Leu Ala Asn Trp
1940 1945 1950
Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Phe Asn Glu Val Leu
1955 1960 1965
Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu Val Asp Tyr Lys Gln
1970 1975 1980
Pro Ile Ile Ile Tyr Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly
1985 1990 1995
Ser Trp Val Val Val Asp Pro Thr Ile Asn Ala Asp Gln Met Glu
2000 2005 2010
Met Tyr Ala Asp Val Asn Ala Arg Ala Gly Val Leu Glu Pro Gln
2015 2020 2025
Gly Met Val Gly Ile Lys Phe Arg Arg Glu Lys Leu Leu Asp Thr
2030 2035 2040
Met Asn Arg Leu Asp Asp Lys Tyr Arg Glu Leu Arg Ser Gln Leu
2045 2050 2055
Ser Asn Lys Ser Leu Ala Pro Glu Val His Gln Gln Ile Ser Lys
2060 2065 2070
Gln Leu Ala Asp Arg Glu Arg Glu Leu Leu Pro Ile Tyr Gly Gln
2075 2080 2085
Ile Ser Leu Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg Met
2090 2095 2100
Val Ala Lys Gly Val Ile Ser Lys Glu Leu Glu Trp Thr Glu Ala
2105 2110 2115
Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg Arg Leu Asn Glu Glu
2120 2125 2130
Tyr Leu Ile Lys Arg Leu Ser His Gln Val Gly Glu Ala Ser Arg
2135 2140 2145
Leu Glu Lys Ile Ala Arg Ile Arg Ser Trp Tyr Pro Ala Ser Val
2150 2155 2160
Asp His Glu Asp Asp Arg Gln Val Ala Thr Trp Ile Glu Glu Asn
2165 2170 2175
Tyr Lys Thr Leu Asp Asp Lys Leu Lys Gly Leu Lys Leu Glu Ser
2180 2185 2190
Phe Ala Gln Asp Leu Ala Lys Lys Ile Arg Ser Asp His Asp Asn
2195 2200 2205
Ala Ile Asp Gly Leu Ser Glu Val Ile Lys Met Leu Ser Thr Asp
2210 2215 2220
Asp Lys Glu Lys Leu Leu Lys Thr Leu Lys
2225 2230
<210> 100
<211> 2233
<212> PRT
<213> ACC1sc_S659A/S1157A
<400> 100
Met Ser Glu Glu Ser Leu Phe Glu Ser Ser Pro Gln Lys Met Glu Tyr
1 5 10 15
Glu Ile Thr Asn Tyr Ser Glu Arg His Thr Glu Leu Pro Gly His Phe
20 25 30
Ile Gly Leu Asn Thr Val Asp Lys Leu Glu Glu Ser Pro Leu Arg Asp
35 40 45
Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile
50 55 60
Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys
65 70 75 80
Trp Ala Tyr Glu Thr Phe Gly Asp Asp Arg Thr Val Gln Phe Val Ala
85 90 95
Met Ala Thr Pro Glu Asp Leu Glu Ala Asn Ala Glu Tyr Ile Arg Met
100 105 110
Ala Asp Gln Tyr Ile Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr
115 120 125
Ala Asn Val Asp Leu Ile Val Asp Ile Ala Glu Arg Ala Asp Val Asp
130 135 140
Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro Leu Leu Pro
145 150 155 160
Glu Lys Leu Ser Gln Ser Lys Arg Lys Val Ile Phe Ile Gly Pro Pro
165 170 175
Gly Asn Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val
180 185 190
Ala Gln Ser Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val
195 200 205
Asp Thr Val His Val Asp Glu Lys Thr Gly Leu Val Ser Val Asp Asp
210 215 220
Asp Ile Tyr Gln Lys Gly Cys Cys Thr Ser Pro Glu Asp Gly Leu Gln
225 230 235 240
Lys Ala Lys Arg Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly
245 250 255
Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile
260 265 270
Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe
275 280 285
Ile Met Lys Leu Ala Gly Arg Ala Arg His Leu Glu Val Gln Leu Leu
290 295 300
Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser
305 310 315 320
Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile
325 330 335
Ala Lys Ala Glu Thr Phe His Glu Met Glu Lys Ala Ala Val Arg Leu
340 345 350
Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr
355 360 365
Ser His Asp Asp Gly Lys Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu
370 375 380
Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro
385 390 395 400
Ala Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser
405 410 415
Asp Ile Arg Thr Leu Tyr Gly Met Asn Pro His Ser Ala Ser Glu Ile
420 425 430
Asp Phe Glu Phe Lys Thr Gln Asp Ala Thr Lys Lys Gln Arg Arg Pro
435 440 445
Ile Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro
450 455 460
Asn Asp Gly Phe Lys Pro Ser Gly Gly Thr Leu His Glu Leu Asn Phe
465 470 475 480
Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly
485 490 495
Asn Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe
500 505 510
Gly Glu Asn Arg Gln Ala Ser Arg Lys His Met Val Val Ala Leu Lys
515 520 525
Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile
530 535 540
Lys Leu Leu Glu Thr Glu Asp Phe Glu Asp Asn Thr Ile Thr Thr Gly
545 550 555 560
Trp Leu Asp Asp Leu Ile Thr His Lys Met Thr Ala Glu Lys Pro Asp
565 570 575
Pro Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Leu Ala
580 585 590
Ser Glu Glu Ala Arg His Lys Tyr Ile Glu Ser Leu Gln Lys Gly Gln
595 600 605
Val Leu Ser Lys Asp Leu Leu Gln Thr Met Phe Pro Val Asp Phe Ile
610 615 620
His Glu Gly Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Gly Asn Asp
625 630 635 640
Arg Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Asp Ile Ile Leu Arg
645 650 655
Gln Leu Ala Asp Gly Gly Leu Leu Ile Ala Ile Gly Gly Lys Ser His
660 665 670
Thr Ile Tyr Trp Lys Glu Glu Val Ala Ala Thr Arg Leu Ser Val Asp
675 680 685
Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg
690 695 700
Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Glu
705 710 715 720
His Ile Ile Lys Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met
725 730 735
Gln Met Pro Leu Val Ser Gln Glu Asn Gly Ile Val Gln Leu Leu Lys
740 745 750
Gln Pro Gly Ser Thr Ile Val Ala Gly Asp Ile Met Ala Ile Met Thr
755 760 765
Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met
770 775 780
Leu Pro Asp Phe Gly Ser Pro Val Ile Glu Gly Thr Lys Pro Ala Tyr
785 790 795 800
Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr
805 810 815
Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val
820 825 830
Leu Arg Asn Pro Lys Leu Pro Tyr Ser Glu Trp Lys Leu His Ile Ser
835 840 845
Ala Leu His Ser Arg Leu Pro Ala Lys Leu Asp Glu Gln Met Glu Glu
850 855 860
Leu Val Ala Arg Ser Leu Arg Arg Gly Ala Val Phe Pro Ala Arg Gln
865 870 875 880
Leu Ser Lys Leu Ile Asp Met Ala Val Lys Asn Pro Glu Tyr Asn Pro
885 890 895
Asp Lys Leu Leu Gly Ala Val Val Glu Pro Leu Ala Asp Ile Ala His
900 905 910
Lys Tyr Ser Asn Gly Leu Glu Ala His Glu His Ser Ile Phe Val His
915 920 925
Phe Leu Glu Glu Tyr Tyr Glu Val Glu Lys Leu Phe Asn Gly Pro Asn
930 935 940
Val Arg Glu Glu Asn Ile Ile Leu Lys Leu Arg Asp Glu Asn Pro Lys
945 950 955 960
Asp Leu Asp Lys Val Ala Leu Thr Val Leu Ser His Ser Lys Val Ser
965 970 975
Ala Lys Asn Asn Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Leu
980 985 990
Cys Lys Leu Ser Ser Lys Val Ser Ala Ile Phe Ser Thr Pro Leu Gln
995 1000 1005
His Ile Val Glu Leu Glu Ser Lys Ala Thr Ala Lys Val Ala Leu
1010 1015 1020
Gln Ala Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys
1025 1030 1035
Glu Arg Thr Glu Gln Ile Glu His Ile Leu Lys Ser Ser Val Val
1040 1045 1050
Lys Val Ala Tyr Gly Ser Ser Asn Pro Lys Arg Ser Glu Pro Asp
1055 1060 1065
Leu Asn Ile Leu Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe
1070 1075 1080
Asp Val Leu Leu Gln Phe Leu Thr His Gln Asp Pro Val Val Thr
1085 1090 1095
Ala Ala Ala Ala Gln Val Tyr Ile Arg Arg Ala Tyr Arg Ala Tyr
1100 1105 1110
Thr Ile Gly Asp Ile Arg Val His Glu Gly Val Thr Val Pro Ile
1115 1120 1125
Val Glu Trp Lys Phe Gln Leu Pro Ser Ala Ala Phe Ser Thr Phe
1130 1135 1140
Pro Thr Val Lys Ser Lys Met Gly Met Asn Arg Ala Val Ala Val
1145 1150 1155
Ser Asp Leu Ser Tyr Val Ala Asn Ser Gln Ser Ser Pro Leu Arg
1160 1165 1170
Glu Gly Ile Leu Met Ala Val Asp His Leu Asp Asp Val Asp Glu
1175 1180 1185
Ile Leu Ser Gln Ser Leu Glu Val Ile Pro Arg His Gln Ser Ser
1190 1195 1200
Ser Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ala Ser Leu
1205 1210 1215
Ser Asn Val Ala Asn Val Cys Val Ala Ser Thr Glu Gly Phe Glu
1220 1225 1230
Ser Glu Glu Glu Ile Leu Val Arg Leu Arg Glu Ile Leu Asp Leu
1235 1240 1245
Asn Lys Gln Glu Leu Ile Asn Ala Ser Ile Arg Arg Ile Thr Phe
1250 1255 1260
Met Phe Gly Phe Lys Asp Gly Ser Tyr Pro Lys Tyr Tyr Thr Phe
1265 1270 1275
Asn Gly Pro Asn Tyr Asn Glu Asn Glu Thr Ile Arg His Ile Glu
1280 1285 1290
Pro Ala Leu Ala Phe Gln Leu Glu Leu Gly Arg Leu Ser Asn Phe
1295 1300 1305
Asn Ile Lys Pro Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr
1310 1315 1320
Glu Ala Val Ser Lys Thr Ser Pro Leu Asp Lys Arg Phe Phe Thr
1325 1330 1335
Arg Gly Ile Ile Arg Thr Gly His Ile Arg Asp Asp Ile Ser Ile
1340 1345 1350
Gln Glu Tyr Leu Thr Ser Glu Ala Asn Arg Leu Met Ser Asp Ile
1355 1360 1365
Leu Asp Asn Leu Glu Val Thr Asp Thr Ser Asn Ser Asp Leu Asn
1370 1375 1380
His Ile Phe Ile Asn Phe Ile Ala Val Phe Asp Ile Ser Pro Glu
1385 1390 1395
Asp Val Glu Ala Ala Phe Gly Gly Phe Leu Glu Arg Phe Gly Lys
1400 1405 1410
Arg Leu Leu Arg Leu Arg Val Ser Ser Ala Glu Ile Arg Ile Ile
1415 1420 1425
Ile Lys Asp Pro Gln Thr Gly Ala Pro Val Pro Leu Arg Ala Leu
1430 1435 1440
Ile Asn Asn Val Ser Gly Tyr Val Ile Lys Thr Glu Met Tyr Thr
1445 1450 1455
Glu Val Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu Gly
1460 1465 1470
Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr Pro
1475 1480 1485
Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met
1490 1495 1500
Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg Gln Ala
1505 1510 1515
Ser Ser Ser Gln Trp Lys Asn Phe Ser Ala Asp Val Lys Leu Thr
1520 1525 1530
Asp Asp Phe Phe Ile Ser Asn Glu Leu Ile Glu Asp Glu Asn Gly
1535 1540 1545
Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala Asn Ala Ile Gly
1550 1555 1560
Met Val Ala Phe Lys Ile Thr Val Lys Thr Pro Glu Tyr Pro Arg
1565 1570 1575
Gly Arg Gln Phe Val Val Val Ala Asn Asp Ile Thr Phe Lys Ile
1580 1585 1590
Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val Thr
1595 1600 1605
Glu Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala
1610 1615 1620
Asn Ser Gly Ala Arg Ile Gly Met Ala Glu Glu Ile Val Pro Leu
1625 1630 1635
Phe Gln Val Ala Trp Asn Asp Ala Ala Asn Pro Asp Lys Gly Phe
1640 1645 1650
Gln Tyr Leu Tyr Leu Thr Ser Glu Gly Met Glu Thr Leu Lys Lys
1655 1660 1665
Phe Asp Lys Glu Asn Ser Val Leu Thr Glu Arg Thr Val Ile Asn
1670 1675 1680
Gly Glu Glu Arg Phe Val Ile Lys Thr Ile Ile Gly Ser Glu Asp
1685 1690 1695
Gly Leu Gly Val Glu Cys Leu Arg Gly Ser Gly Leu Ile Ala Gly
1700 1705 1710
Ala Thr Ser Arg Ala Tyr His Asp Ile Phe Thr Ile Thr Leu Val
1715 1720 1725
Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val Arg Leu Gly
1730 1735 1740
Gln Arg Ala Ile Gln Val Glu Gly Gln Pro Ile Ile Leu Thr Gly
1745 1750 1755
Ala Pro Ala Ile Asn Lys Met Leu Gly Arg Glu Val Tyr Thr Ser
1760 1765 1770
Asn Leu Gln Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val
1775 1780 1785
Ser His Leu Thr Ala Val Asp Asp Leu Ala Gly Val Glu Lys Ile
1790 1795 1800
Val Glu Trp Met Ser Tyr Val Pro Ala Lys Arg Asn Met Pro Val
1805 1810 1815
Pro Ile Leu Glu Thr Lys Asp Thr Trp Asp Arg Pro Val Asp Phe
1820 1825 1830
Thr Pro Thr Asn Asp Glu Thr Tyr Asp Val Arg Trp Met Ile Glu
1835 1840 1845
Gly Arg Glu Thr Glu Ser Gly Phe Glu Tyr Gly Leu Phe Asp Lys
1850 1855 1860
Gly Ser Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val
1865 1870 1875
Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Leu Gly Val Ile Gly
1880 1885 1890
Val Glu Thr Arg Thr Val Glu Asn Leu Ile Pro Ala Asp Pro Ala
1895 1900 1905
Asn Pro Asn Ser Ala Glu Thr Leu Ile Gln Glu Pro Gly Gln Val
1910 1915 1920
Trp His Pro Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile Asn Asp
1925 1930 1935
Phe Asn Asn Gly Glu Gln Leu Pro Met Met Ile Leu Ala Asn Trp
1940 1945 1950
Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Phe Asn Glu Val Leu
1955 1960 1965
Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu Val Asp Tyr Lys Gln
1970 1975 1980
Pro Ile Ile Ile Tyr Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly
1985 1990 1995
Ser Trp Val Val Val Asp Pro Thr Ile Asn Ala Asp Gln Met Glu
2000 2005 2010
Met Tyr Ala Asp Val Asn Ala Arg Ala Gly Val Leu Glu Pro Gln
2015 2020 2025
Gly Met Val Gly Ile Lys Phe Arg Arg Glu Lys Leu Leu Asp Thr
2030 2035 2040
Met Asn Arg Leu Asp Asp Lys Tyr Arg Glu Leu Arg Ser Gln Leu
2045 2050 2055
Ser Asn Lys Ser Leu Ala Pro Glu Val His Gln Gln Ile Ser Lys
2060 2065 2070
Gln Leu Ala Asp Arg Glu Arg Glu Leu Leu Pro Ile Tyr Gly Gln
2075 2080 2085
Ile Ser Leu Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg Met
2090 2095 2100
Val Ala Lys Gly Val Ile Ser Lys Glu Leu Glu Trp Thr Glu Ala
2105 2110 2115
Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg Arg Leu Asn Glu Glu
2120 2125 2130
Tyr Leu Ile Lys Arg Leu Ser His Gln Val Gly Glu Ala Ser Arg
2135 2140 2145
Leu Glu Lys Ile Ala Arg Ile Arg Ser Trp Tyr Pro Ala Ser Val
2150 2155 2160
Asp His Glu Asp Asp Arg Gln Val Ala Thr Trp Ile Glu Glu Asn
2165 2170 2175
Tyr Lys Thr Leu Asp Asp Lys Leu Lys Gly Leu Lys Leu Glu Ser
2180 2185 2190
Phe Ala Gln Asp Leu Ala Lys Lys Ile Arg Ser Asp His Asp Asn
2195 2200 2205
Ala Ile Asp Gly Leu Ser Glu Val Ile Lys Met Leu Ser Thr Asp
2210 2215 2220
Asp Lys Glu Lys Leu Leu Lys Thr Leu Lys
2225 2230
<210> 101
<211> 2233
<212> PRT
<213> ACC1sc_S1157A
<400> 101
Met Ser Glu Glu Ser Leu Phe Glu Ser Ser Pro Gln Lys Met Glu Tyr
1 5 10 15
Glu Ile Thr Asn Tyr Ser Glu Arg His Thr Glu Leu Pro Gly His Phe
20 25 30
Ile Gly Leu Asn Thr Val Asp Lys Leu Glu Glu Ser Pro Leu Arg Asp
35 40 45
Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile
50 55 60
Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys
65 70 75 80
Trp Ala Tyr Glu Thr Phe Gly Asp Asp Arg Thr Val Gln Phe Val Ala
85 90 95
Met Ala Thr Pro Glu Asp Leu Glu Ala Asn Ala Glu Tyr Ile Arg Met
100 105 110
Ala Asp Gln Tyr Ile Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr
115 120 125
Ala Asn Val Asp Leu Ile Val Asp Ile Ala Glu Arg Ala Asp Val Asp
130 135 140
Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro Leu Leu Pro
145 150 155 160
Glu Lys Leu Ser Gln Ser Lys Arg Lys Val Ile Phe Ile Gly Pro Pro
165 170 175
Gly Asn Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val
180 185 190
Ala Gln Ser Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val
195 200 205
Asp Thr Val His Val Asp Glu Lys Thr Gly Leu Val Ser Val Asp Asp
210 215 220
Asp Ile Tyr Gln Lys Gly Cys Cys Thr Ser Pro Glu Asp Gly Leu Gln
225 230 235 240
Lys Ala Lys Arg Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly
245 250 255
Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile
260 265 270
Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe
275 280 285
Ile Met Lys Leu Ala Gly Arg Ala Arg His Leu Glu Val Gln Leu Leu
290 295 300
Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser
305 310 315 320
Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile
325 330 335
Ala Lys Ala Glu Thr Phe His Glu Met Glu Lys Ala Ala Val Arg Leu
340 345 350
Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr
355 360 365
Ser His Asp Asp Gly Lys Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu
370 375 380
Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro
385 390 395 400
Ala Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser
405 410 415
Asp Ile Arg Thr Leu Tyr Gly Met Asn Pro His Ser Ala Ser Glu Ile
420 425 430
Asp Phe Glu Phe Lys Thr Gln Asp Ala Thr Lys Lys Gln Arg Arg Pro
435 440 445
Ile Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro
450 455 460
Asn Asp Gly Phe Lys Pro Ser Gly Gly Thr Leu His Glu Leu Asn Phe
465 470 475 480
Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly
485 490 495
Asn Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe
500 505 510
Gly Glu Asn Arg Gln Ala Ser Arg Lys His Met Val Val Ala Leu Lys
515 520 525
Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile
530 535 540
Lys Leu Leu Glu Thr Glu Asp Phe Glu Asp Asn Thr Ile Thr Thr Gly
545 550 555 560
Trp Leu Asp Asp Leu Ile Thr His Lys Met Thr Ala Glu Lys Pro Asp
565 570 575
Pro Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Leu Ala
580 585 590
Ser Glu Glu Ala Arg His Lys Tyr Ile Glu Ser Leu Gln Lys Gly Gln
595 600 605
Val Leu Ser Lys Asp Leu Leu Gln Thr Met Phe Pro Val Asp Phe Ile
610 615 620
His Glu Gly Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Gly Asn Asp
625 630 635 640
Arg Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Asp Ile Ile Leu Arg
645 650 655
Gln Leu Ser Asp Gly Gly Leu Leu Ile Ala Ile Gly Gly Lys Ser His
660 665 670
Thr Ile Tyr Trp Lys Glu Glu Val Ala Ala Thr Arg Leu Ser Val Asp
675 680 685
Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg
690 695 700
Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Glu
705 710 715 720
His Ile Ile Lys Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met
725 730 735
Gln Met Pro Leu Val Ser Gln Glu Asn Gly Ile Val Gln Leu Leu Lys
740 745 750
Gln Pro Gly Ser Thr Ile Val Ala Gly Asp Ile Met Ala Ile Met Thr
755 760 765
Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met
770 775 780
Leu Pro Asp Phe Gly Ser Pro Val Ile Glu Gly Thr Lys Pro Ala Tyr
785 790 795 800
Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr
805 810 815
Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val
820 825 830
Leu Arg Asn Pro Lys Leu Pro Tyr Ser Glu Trp Lys Leu His Ile Ser
835 840 845
Ala Leu His Ser Arg Leu Pro Ala Lys Leu Asp Glu Gln Met Glu Glu
850 855 860
Leu Val Ala Arg Ser Leu Arg Arg Gly Ala Val Phe Pro Ala Arg Gln
865 870 875 880
Leu Ser Lys Leu Ile Asp Met Ala Val Lys Asn Pro Glu Tyr Asn Pro
885 890 895
Asp Lys Leu Leu Gly Ala Val Val Glu Pro Leu Ala Asp Ile Ala His
900 905 910
Lys Tyr Ser Asn Gly Leu Glu Ala His Glu His Ser Ile Phe Val His
915 920 925
Phe Leu Glu Glu Tyr Tyr Glu Val Glu Lys Leu Phe Asn Gly Pro Asn
930 935 940
Val Arg Glu Glu Asn Ile Ile Leu Lys Leu Arg Asp Glu Asn Pro Lys
945 950 955 960
Asp Leu Asp Lys Val Ala Leu Thr Val Leu Ser His Ser Lys Val Ser
965 970 975
Ala Lys Asn Asn Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Leu
980 985 990
Cys Lys Leu Ser Ser Lys Val Ser Ala Ile Phe Ser Thr Pro Leu Gln
995 1000 1005
His Ile Val Glu Leu Glu Ser Lys Ala Thr Ala Lys Val Ala Leu
1010 1015 1020
Gln Ala Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys
1025 1030 1035
Glu Arg Thr Glu Gln Ile Glu His Ile Leu Lys Ser Ser Val Val
1040 1045 1050
Lys Val Ala Tyr Gly Ser Ser Asn Pro Lys Arg Ser Glu Pro Asp
1055 1060 1065
Leu Asn Ile Leu Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe
1070 1075 1080
Asp Val Leu Leu Gln Phe Leu Thr His Gln Asp Pro Val Val Thr
1085 1090 1095
Ala Ala Ala Ala Gln Val Tyr Ile Arg Arg Ala Tyr Arg Ala Tyr
1100 1105 1110
Thr Ile Gly Asp Ile Arg Val His Glu Gly Val Thr Val Pro Ile
1115 1120 1125
Val Glu Trp Lys Phe Gln Leu Pro Ser Ala Ala Phe Ser Thr Phe
1130 1135 1140
Pro Thr Val Lys Ser Lys Met Gly Met Asn Arg Ala Val Ala Val
1145 1150 1155
Ser Asp Leu Ser Tyr Val Ala Asn Ser Gln Ser Ser Pro Leu Arg
1160 1165 1170
Glu Gly Ile Leu Met Ala Val Asp His Leu Asp Asp Val Asp Glu
1175 1180 1185
Ile Leu Ser Gln Ser Leu Glu Val Ile Pro Arg His Gln Ser Ser
1190 1195 1200
Ser Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ala Ser Leu
1205 1210 1215
Ser Asn Val Ala Asn Val Cys Val Ala Ser Thr Glu Gly Phe Glu
1220 1225 1230
Ser Glu Glu Glu Ile Leu Val Arg Leu Arg Glu Ile Leu Asp Leu
1235 1240 1245
Asn Lys Gln Glu Leu Ile Asn Ala Ser Ile Arg Arg Ile Thr Phe
1250 1255 1260
Met Phe Gly Phe Lys Asp Gly Ser Tyr Pro Lys Tyr Tyr Thr Phe
1265 1270 1275
Asn Gly Pro Asn Tyr Asn Glu Asn Glu Thr Ile Arg His Ile Glu
1280 1285 1290
Pro Ala Leu Ala Phe Gln Leu Glu Leu Gly Arg Leu Ser Asn Phe
1295 1300 1305
Asn Ile Lys Pro Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr
1310 1315 1320
Glu Ala Val Ser Lys Thr Ser Pro Leu Asp Lys Arg Phe Phe Thr
1325 1330 1335
Arg Gly Ile Ile Arg Thr Gly His Ile Arg Asp Asp Ile Ser Ile
1340 1345 1350
Gln Glu Tyr Leu Thr Ser Glu Ala Asn Arg Leu Met Ser Asp Ile
1355 1360 1365
Leu Asp Asn Leu Glu Val Thr Asp Thr Ser Asn Ser Asp Leu Asn
1370 1375 1380
His Ile Phe Ile Asn Phe Ile Ala Val Phe Asp Ile Ser Pro Glu
1385 1390 1395
Asp Val Glu Ala Ala Phe Gly Gly Phe Leu Glu Arg Phe Gly Lys
1400 1405 1410
Arg Leu Leu Arg Leu Arg Val Ser Ser Ala Glu Ile Arg Ile Ile
1415 1420 1425
Ile Lys Asp Pro Gln Thr Gly Ala Pro Val Pro Leu Arg Ala Leu
1430 1435 1440
Ile Asn Asn Val Ser Gly Tyr Val Ile Lys Thr Glu Met Tyr Thr
1445 1450 1455
Glu Val Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu Gly
1460 1465 1470
Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr Pro
1475 1480 1485
Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met
1490 1495 1500
Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg Gln Ala
1505 1510 1515
Ser Ser Ser Gln Trp Lys Asn Phe Ser Ala Asp Val Lys Leu Thr
1520 1525 1530
Asp Asp Phe Phe Ile Ser Asn Glu Leu Ile Glu Asp Glu Asn Gly
1535 1540 1545
Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala Asn Ala Ile Gly
1550 1555 1560
Met Val Ala Phe Lys Ile Thr Val Lys Thr Pro Glu Tyr Pro Arg
1565 1570 1575
Gly Arg Gln Phe Val Val Val Ala Asn Asp Ile Thr Phe Lys Ile
1580 1585 1590
Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val Thr
1595 1600 1605
Glu Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala
1610 1615 1620
Asn Ser Gly Ala Arg Ile Gly Met Ala Glu Glu Ile Val Pro Leu
1625 1630 1635
Phe Gln Val Ala Trp Asn Asp Ala Ala Asn Pro Asp Lys Gly Phe
1640 1645 1650
Gln Tyr Leu Tyr Leu Thr Ser Glu Gly Met Glu Thr Leu Lys Lys
1655 1660 1665
Phe Asp Lys Glu Asn Ser Val Leu Thr Glu Arg Thr Val Ile Asn
1670 1675 1680
Gly Glu Glu Arg Phe Val Ile Lys Thr Ile Ile Gly Ser Glu Asp
1685 1690 1695
Gly Leu Gly Val Glu Cys Leu Arg Gly Ser Gly Leu Ile Ala Gly
1700 1705 1710
Ala Thr Ser Arg Ala Tyr His Asp Ile Phe Thr Ile Thr Leu Val
1715 1720 1725
Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val Arg Leu Gly
1730 1735 1740
Gln Arg Ala Ile Gln Val Glu Gly Gln Pro Ile Ile Leu Thr Gly
1745 1750 1755
Ala Pro Ala Ile Asn Lys Met Leu Gly Arg Glu Val Tyr Thr Ser
1760 1765 1770
Asn Leu Gln Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val
1775 1780 1785
Ser His Leu Thr Ala Val Asp Asp Leu Ala Gly Val Glu Lys Ile
1790 1795 1800
Val Glu Trp Met Ser Tyr Val Pro Ala Lys Arg Asn Met Pro Val
1805 1810 1815
Pro Ile Leu Glu Thr Lys Asp Thr Trp Asp Arg Pro Val Asp Phe
1820 1825 1830
Thr Pro Thr Asn Asp Glu Thr Tyr Asp Val Arg Trp Met Ile Glu
1835 1840 1845
Gly Arg Glu Thr Glu Ser Gly Phe Glu Tyr Gly Leu Phe Asp Lys
1850 1855 1860
Gly Ser Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val
1865 1870 1875
Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Leu Gly Val Ile Gly
1880 1885 1890
Val Glu Thr Arg Thr Val Glu Asn Leu Ile Pro Ala Asp Pro Ala
1895 1900 1905
Asn Pro Asn Ser Ala Glu Thr Leu Ile Gln Glu Pro Gly Gln Val
1910 1915 1920
Trp His Pro Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile Asn Asp
1925 1930 1935
Phe Asn Asn Gly Glu Gln Leu Pro Met Met Ile Leu Ala Asn Trp
1940 1945 1950
Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Phe Asn Glu Val Leu
1955 1960 1965
Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu Val Asp Tyr Lys Gln
1970 1975 1980
Pro Ile Ile Ile Tyr Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly
1985 1990 1995
Ser Trp Val Val Val Asp Pro Thr Ile Asn Ala Asp Gln Met Glu
2000 2005 2010
Met Tyr Ala Asp Val Asn Ala Arg Ala Gly Val Leu Glu Pro Gln
2015 2020 2025
Gly Met Val Gly Ile Lys Phe Arg Arg Glu Lys Leu Leu Asp Thr
2030 2035 2040
Met Asn Arg Leu Asp Asp Lys Tyr Arg Glu Leu Arg Ser Gln Leu
2045 2050 2055
Ser Asn Lys Ser Leu Ala Pro Glu Val His Gln Gln Ile Ser Lys
2060 2065 2070
Gln Leu Ala Asp Arg Glu Arg Glu Leu Leu Pro Ile Tyr Gly Gln
2075 2080 2085
Ile Ser Leu Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg Met
2090 2095 2100
Val Ala Lys Gly Val Ile Ser Lys Glu Leu Glu Trp Thr Glu Ala
2105 2110 2115
Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg Arg Leu Asn Glu Glu
2120 2125 2130
Tyr Leu Ile Lys Arg Leu Ser His Gln Val Gly Glu Ala Ser Arg
2135 2140 2145
Leu Glu Lys Ile Ala Arg Ile Arg Ser Trp Tyr Pro Ala Ser Val
2150 2155 2160
Asp His Glu Asp Asp Arg Gln Val Ala Thr Trp Ile Glu Glu Asn
2165 2170 2175
Tyr Lys Thr Leu Asp Asp Lys Leu Lys Gly Leu Lys Leu Glu Ser
2180 2185 2190
Phe Ala Gln Asp Leu Ala Lys Lys Ile Arg Ser Asp His Asp Asn
2195 2200 2205
Ala Ile Asp Gly Leu Ser Glu Val Ile Lys Met Leu Ser Thr Asp
2210 2215 2220
Asp Lys Glu Lys Leu Leu Lys Thr Leu Lys
2225 2230
<210> 102
<211> 2248
<212> PRT
<213> ACC1ke
<400> 102
Met Ser Glu Glu Asn Leu Phe Glu Asp Ser Ser Ala Lys Arg Gln Tyr
1 5 10 15
Glu Ile Thr Asp Tyr Ser Lys Lys His Lys Ala Leu Ala Pro His Phe
20 25 30
Val Gly Leu Asn Thr Leu Asp Lys Val Glu Glu Ser Pro Leu Lys Asp
35 40 45
Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile
50 55 60
Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys
65 70 75 80
Trp Ser Tyr Glu Thr Phe Gly Asp Glu Arg Thr Val Gln Phe Val Ala
85 90 95
Met Ala Thr Pro Glu Asp Leu Gln Ala Asn Ser Glu Tyr Ile Arg Met
100 105 110
Ala Asp Gln Tyr Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr
115 120 125
Ala Asn Val Asp Leu Ile Val Asp Val Ala Glu Arg Thr Asp Val Asp
130 135 140
Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro His Leu Pro
145 150 155 160
Glu Lys Leu Ala Ala Ser Lys Arg Lys Ile Ile Phe Ile Gly Pro Pro
165 170 175
Gly Ser Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val
180 185 190
Ala Gln His Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Ile
195 200 205
Asp Lys Val His Val Asp Glu Thr Ser Gly Leu Val Ser Val Asp Asp
210 215 220
Asp Val Tyr Gln Gln Gly Cys Cys Gln Ser Pro Glu Asp Gly Leu Ala
225 230 235 240
Lys Ala Lys Gln Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly
245 250 255
Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile
260 265 270
Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe
275 280 285
Ile Met Lys Leu Ala Gly Lys Ala Arg His Leu Glu Val Gln Leu Leu
290 295 300
Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser
305 310 315 320
Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile
325 330 335
Ala Asn Asn Glu Thr Phe Asp Lys Met Ala Asn Ala Ala Val Arg Leu
340 345 350
Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr
355 360 365
Ser Asn Glu Glu Lys Lys Tyr Tyr Phe Leu Glu Leu Asn Pro Arg Leu
370 375 380
Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro
385 390 395 400
Ser Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser
405 410 415
Asp Ile Arg Val Leu Tyr Gly Met Asn Pro His Ser Ala Thr Glu Ile
420 425 430
Asp Phe Glu Phe Lys Thr Glu Glu Ala Lys Lys Thr Gln Arg Lys Pro
435 440 445
Val Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro
450 455 460
Asn Glu Gly Phe Lys Pro Ser Gly Gly Ser Leu His Glu Leu Asn Phe
465 470 475 480
Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly
485 490 495
Gly Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe
500 505 510
Gly Glu Asn Arg Gln Met Ser Arg Lys His Met Val Val Ala Leu Lys
515 520 525
Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile
530 535 540
Lys Leu Leu Glu Thr Glu Asp Phe Glu Glu Asn Thr Ile Thr Thr Gly
545 550 555 560
Trp Leu Asp Asp Leu Ile Ser His Lys Met Thr Ala Glu Lys Pro Asp
565 570 575
Ala Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Ile Ala
580 585 590
Ser Glu Ser Ala Arg Met Glu Tyr Ile Ala Gln Leu Lys Arg Gly Gln
595 600 605
Val Pro Ala Lys Asn Leu Leu Glu Thr Met Phe Pro Val Glu Phe Ile
610 615 620
His Glu Asn Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Ala Asp Asp
625 630 635 640
Ser Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Glu Val Arg Ala Val
645 650 655
Lys Leu Ser Asp Gly Gly Leu Leu Ile Ser Val Gly Gly Gln Ser His
660 665 670
Thr Ile Tyr Trp Lys Glu Glu Ala Gln Ala Thr Arg Leu Ser Ile Asp
675 680 685
Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg
690 695 700
Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Asp
705 710 715 720
Gln Ile Lys Val Gly Gln Ala Tyr Ala Glu Ile Glu Val Met Lys Met
725 730 735
Gln Met Pro Leu Val Ser Gln Glu Asn Gly Val Ile Gln Leu Leu Lys
740 745 750
Gln Pro Gly Ser Thr Ile Ala Ala Gly Asp Ile Ile Ala Met Leu Ala
755 760 765
Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met
770 775 780
Leu Pro Glu Leu Gly Ser Pro Met Val Glu Gly Thr Lys Pro Ala Tyr
785 790 795 800
Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr
805 810 815
Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val
820 825 830
Leu Arg Asp Pro Arg Leu Pro Tyr Ser Glu Trp Lys Leu Cys Val Ser
835 840 845
Ala Leu His Ser Arg Leu Pro Met Ser Leu Asp Glu Lys Leu Asp Gln
850 855 860
Leu Val Asp Arg Ser Ser Asn Arg Asn Ala Ile Phe Pro Ala Lys Gln
865 870 875 880
Leu Asn Lys Met Ile Glu Asp Ala Val Arg Glu Pro Gly Ser Asp Glu
885 890 895
Leu Leu Pro Thr Ile Val Glu Pro Leu Leu Asp Ile Thr Asn Arg Tyr
900 905 910
Ala Asn Gly Leu Glu Ala His Glu His Ser Val Phe Val Lys Phe Leu
915 920 925
Glu Glu Tyr Tyr Asn Val Glu Lys Leu Phe Ser Ser His Gly Asn Arg
930 935 940
Glu Glu Asn Val Ile Leu Lys Leu Arg Asp Glu Asn Leu Asp Ser Leu
945 950 955 960
Asp Lys Val Val Glu Ile Val Leu Ser His Ala Lys Val Ser Ala Lys
965 970 975
Asn Thr Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Ile Cys Lys
980 985 990
Met Ser Ser Ser Val Ala His Ile Phe Ser Lys Ser Leu Gly His Ile
995 1000 1005
Val Glu Leu Glu Ser Lys Ser Ala Ala Lys Val Ala Leu Gln Ala
1010 1015 1020
Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg
1025 1030 1035
Thr Glu Gln Ile Glu His Ile Leu Arg Ser Ser Val Thr Asn Thr
1040 1045 1050
Ser Tyr Gly Asp Leu Ala Pro Lys Lys Ser Glu Pro Asn Leu Glu
1055 1060 1065
Ile Leu Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val
1070 1075 1080
Leu Leu Pro Phe Leu Cys His Glu Asp Pro Val Ile Ala Thr Ala
1085 1090 1095
Ala Ala Gln Val Tyr Val Arg Arg Ala Tyr Arg Ala Tyr Thr Val
1100 1105 1110
Gly Glu Val Lys Thr Pro Asn Ile Lys Asp Ser Ser Ile Ser Pro
1115 1120 1125
Leu Ile Glu Trp Lys Phe Gln Leu Pro Ser Ala Ala Phe Ser Ser
1130 1135 1140
Ile Pro Gln Val Lys Ser Lys Ile Gly Met Asn Arg Ala Met Ser
1145 1150 1155
Val Ser Asp Leu Ser Tyr Val Val Asp Asn Glu His Ala Pro Leu
1160 1165 1170
Arg Thr Gly Ile Leu Val Ala Ser Glu His Leu Asp Asp Val Asp
1175 1180 1185
Ala Cys Leu Ala Gln Gly Leu Glu Ala Ile Pro Glu His Ala Asn
1190 1195 1200
Thr Ala Asn Gly Pro Ala Pro Asp Arg Ser Gly Asn Ser Ser Ser
1205 1210 1215
Leu Ser Asn Val Ala Asn Val Cys Val Ser Ser Thr Glu Gly Phe
1220 1225 1230
Glu Ser Glu Lys Asp Ile Leu Ala Arg Val His Glu Ile Leu Asp
1235 1240 1245
Leu Asn Lys Ser Asp Leu Ile Lys Ser Ser Ile Arg Arg Ile Thr
1250 1255 1260
Phe Met Phe Gly Phe Ala Asp Gly Thr Tyr Pro Lys Tyr Tyr Thr
1265 1270 1275
Phe Asn Gly Pro Asn Tyr Gln Glu Asn Gln Thr Ile Arg His Ile
1280 1285 1290
Glu Pro Ala Cys Ala Phe Gln Leu Glu Leu Gly Arg Met Ser Asn
1295 1300 1305
Phe Asn Ile Lys Pro Ile Phe Thr Glu Asn Arg Asn Ile His Val
1310 1315 1320
Tyr Glu Ala Ile Ser Lys Thr Ser Ala Leu Asp Lys Arg Phe Phe
1325 1330 1335
Thr Arg Gly Ile Ile Arg Thr Gly Arg Ile Arg Asp Glu Ile Ser
1340 1345 1350
Ile Gln Glu Tyr Leu Thr Ser Glu Ala Asn Lys Leu Met Ser Asp
1355 1360 1365
Ile Leu Asp Asn Leu Glu Ile Val Asp Thr Ser Asn Ser Asp Leu
1370 1375 1380
Asn His Ile Phe Ile Asn Phe Ser Ala Val Phe Asp Val Ser Pro
1385 1390 1395
Glu Asp Val Glu Ala Ala Phe Gly Gly Phe Leu Glu Arg Phe Gly
1400 1405 1410
Lys Arg Phe Leu Arg Leu Arg Val Ser Ala Ala Glu Ile Arg Ile
1415 1420 1425
Ile Ile Gln Asp Pro Lys Thr Gly Ala Ala Val Pro Leu Arg Ala
1430 1435 1440
Leu Ile Asn Asn Val Ser Gly Tyr Val Val Lys Ser Glu Leu Tyr
1445 1450 1455
Thr Glu Ile Lys Asn Asp Lys Gly Glu Trp Val Phe Lys Ser Leu
1460 1465 1470
Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr
1475 1480 1485
Pro Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu
1490 1495 1500
Met Gly Thr Thr Tyr Leu Tyr Asp Phe Pro Glu Leu Phe His Gln
1505 1510 1515
Ala Ala Ile Thr Thr Trp Glu Lys Tyr Leu Ser Asn Ser Ser Ala
1520 1525 1530
Lys Glu Leu Ser Ser Ile Lys Asp Arg Asp Asp Leu Phe Ile Ser
1535 1540 1545
His Glu Leu Ile Glu Asp Glu Asn Gly Glu Leu Thr Glu Val Glu
1550 1555 1560
Arg Glu Pro Gly Ala Asn Thr Ile Gly Met Val Ala Phe Lys Val
1565 1570 1575
Thr Met Lys Thr Pro Glu Tyr Pro Arg Gly Arg Gln Phe Val Ile
1580 1585 1590
Val Ala Asn Asp Ile Thr Phe Lys Ile Gly Ser Phe Gly Pro Gln
1595 1600 1605
Glu Asp Glu Phe Phe Asn Lys Val Thr Glu Tyr Ala Arg Lys Arg
1610 1615 1620
Gly Ile Pro Arg Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg Ile
1625 1630 1635
Gly Ile Ala Glu Glu Leu Val Pro Leu Phe Gln Val Ala Trp Lys
1640 1645 1650
Asp Ser Lys Val Pro Ala Lys Gly Phe Glu Tyr Leu Tyr Leu Ser
1655 1660 1665
Lys Glu Gly Met Glu Thr Leu Lys Ser Tyr Ser Lys Gln Asn Ser
1670 1675 1680
Val Ile Thr Glu Arg Val Val Glu Glu Gly Glu Glu Arg Tyr Val
1685 1690 1695
Ile Lys Ser Ile Ile Gly Ala Asp Asp Gly Leu Gly Val Glu Cys
1700 1705 1710
Leu Arg Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr
1715 1720 1725
Gln Asp Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly
1730 1735 1740
Ile Gly Ala Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Ile
1745 1750 1755
Glu Gly Gln Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys
1760 1765 1770
Val Leu Gly Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly
1775 1780 1785
Thr Gln Ile Met Tyr Asn Asn Gly Val Ser His Leu Thr Ala Ser
1790 1795 1800
Asp Asp Leu Gln Ala Val Glu Gln Ile Ile Asn Trp Leu Ser Tyr
1805 1810 1815
Val Pro Ala Lys Arg Asn Met Pro Val Pro Ile Leu Glu Thr Glu
1820 1825 1830
Asp Lys Trp Asp Arg Ala Ile Asp Phe Arg Pro Thr Thr Asn Glu
1835 1840 1845
Gln Tyr Asp Val Arg Trp Leu Ile Gln Gly Arg Glu Leu Asp Asn
1850 1855 1860
Gly Asp Phe Glu Tyr Gly Met Phe Asp Lys Gly Ser Phe Phe Glu
1865 1870 1875
Thr Leu Ser Gly Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg
1880 1885 1890
Leu Gly Gly Ile Pro Ile Gly Val Ile Gly Val Glu Thr Arg Thr
1895 1900 1905
Ile Glu Asn Val Ile Pro Ala Asp Pro Ala Asn Pro Asp Ser Thr
1910 1915 1920
Glu Thr Val Val Gln Glu Ala Gly Gln Val Trp Tyr Pro Asn Ser
1925 1930 1935
Ala Phe Lys Thr Ala Gln Ala Ile Gln Asp Phe Asn His Gly Glu
1940 1945 1950
Gln Leu Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly
1955 1960 1965
Gly Gln Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe
1970 1975 1980
Ile Val Asp Ala Leu Val Asp Tyr Lys Gln Pro Ile Phe Ile Tyr
1985 1990 1995
Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp Val Val Val
2000 2005 2010
Asp Pro Thr Ile Asn Asn Asp His Met Glu Met Tyr Ala Asp Lys
2015 2020 2025
Asp Ser Arg Ala Gly Val Leu Glu Pro Ser Gly Met Val Gly Ile
2030 2035 2040
Lys Tyr Arg Arg Glu Lys Leu Leu Ser Thr Ile Thr Arg Leu Asp
2045 2050 2055
Ser Thr Tyr Arg Glu Leu Lys Glu Lys Leu Ser Asp Lys Leu Leu
2060 2065 2070
Ser Thr Glu Glu His Gln Lys Ile Ser Ile Gln Leu Ala Glu Arg
2075 2080 2085
Glu Lys Met Leu Ile Pro Ile Tyr Asn Gln Ile Ser Val Gln Phe
2090 2095 2100
Ala Asp Leu His Asp Arg Ser Ser Arg Met Leu Lys Lys Gly Val
2105 2110 2115
Ile Lys Ala Glu Leu Thr Trp Glu Glu Ser Arg Arg Tyr Phe Phe
2120 2125 2130
Trp Arg Leu Arg Arg Arg Leu Asp Glu Glu Tyr Leu Leu Arg Arg
2135 2140 2145
Leu Asp Ser Gln Leu Thr Gln Ala Ser Arg Leu Glu Lys Ile Ala
2150 2155 2160
Arg Val Arg Ser Trp Tyr Pro Thr Ser Val Asp Val Glu Asn Asp
2165 2170 2175
Lys Glu Val Val Thr Trp Ile Glu Glu Asn Tyr Glu Thr Leu Glu
2180 2185 2190
Glu Lys Phe Lys Ala Leu Lys Leu Glu Ser Phe Ala Leu Asp Leu
2195 2200 2205
Ala Lys Asn Ile Arg Asn Asp His Glu Asn Ser Met Lys Gly Leu
2210 2215 2220
Glu Glu Val Val Arg Met Leu Ser Ala Thr Asp Lys Glu Arg Leu
2225 2230 2235
Asn Lys Ile Ile Asn Pro Asp Asp Lys Lys
2240 2245
<210> 103
<211> 2216
<212> PRT
<213> ACC1mc
<400> 103
Met Val Glu His Lys Ser Leu Pro Gly His Phe Leu Gly Gly Asn Ser
1 5 10 15
Leu Glu Ser Ala Pro Gln Gly Pro Val Lys Asp Phe Val Gln Ala His
20 25 30
Glu Gly His Thr Val Ile Ser Lys Val Leu Ile Ala Asn Asn Gly Met
35 40 45
Ala Ala Met Lys Glu Ile Arg Ser Val Arg Lys Trp Ala Tyr Glu Thr
50 55 60
Phe Gly Asn Glu Arg Ala Ile Glu Phe Thr Val Met Ala Thr Pro Glu
65 70 75 80
Asp Leu Lys Ala Asn Ala Glu Tyr Ile Arg Met Ala Asp Asn Phe Val
85 90 95
Glu Val Pro Gly Gly Ser Asn Asn Asn Asn Tyr Ala Asn Val Glu Leu
100 105 110
Ile Val Asp Val Ala Glu Arg Thr Ala Val His Ala Val Trp Ala Gly
115 120 125
Trp Gly His Ala Ser Glu Asn Pro Arg Leu Pro Glu Met Leu Ala Lys
130 135 140
Ser Lys His Lys Cys Leu Phe Ile Gly Pro Pro Ala Ser Ala Met Arg
145 150 155 160
Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val Ala Gln Ser Ala Gln
165 170 175
Val Pro Thr Met Gly Trp Ser Gly Asp Gly Ile Thr Glu Thr Glu Phe
180 185 190
Asp Ala Ala Gly His Val Ile Val Pro Asp Asn Ala Tyr Asn Glu Ala
195 200 205
Cys Val Lys Thr Ala Glu Gln Gly Leu Lys Ala Ala Glu Lys Ile Gly
210 215 220
Phe Pro Val Met Ile Lys Ala Ser Glu Gly Gly Gly Gly Lys Gly Ile
225 230 235 240
Arg Met Val Lys Asp Gly Ser Asn Phe Ala Gln Leu Phe Ala Gln Val
245 250 255
Gln Gly Glu Ile Pro Gly Ser Pro Ile Phe Ile Met Lys Leu Ala Gly
260 265 270
Asn Ala Arg His Leu Glu Val Gln Leu Leu Ala Asp Gln Tyr Gly Asn
275 280 285
Ala Ile Ser Leu Phe Gly Arg Asp Cys Ser Val Gln Arg Arg His Gln
290 295 300
Lys Ile Ile Glu Glu Ala Pro Val Thr Ile Ala Lys Pro Asp Val Phe
305 310 315 320
Glu Gln Met Glu Lys Ala Ala Val Arg Leu Gly Lys Leu Val Gly Tyr
325 330 335
Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr Ser His His Asp Asp Gln
340 345 350
Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Thr
355 360 365
Thr Glu Met Val Ser Gly Val Asn Leu Pro Ala Ala Gln Leu Gln Ile
370 375 380
Ala Met Gly Ile Pro Leu His Arg Ile Arg Asp Ile Arg Val Leu Tyr
385 390 395 400
Gly Val Gln Pro Asn Ser Ala Ser Glu Ile Asp Phe Gly Phe Glu His
405 410 415
Pro Thr Ser Leu Thr Ser His Arg Arg Pro Thr Pro Lys Gly His Val
420 425 430
Ile Ala Cys Arg Ile Thr Ala Glu Asn Pro Asp Ala Gly Phe Lys Pro
435 440 445
Ser Ser Gly Ile Met Gln Glu Leu Asn Phe Arg Ser Ser Thr Asn Val
450 455 460
Trp Gly Tyr Phe Ser Val Val Ser Ala Gly Gly Leu His Glu Tyr Ala
465 470 475 480
Asp Ser Gln Phe Gly His Ile Phe Ala Tyr Gly Glu Asn Arg Gln Gln
485 490 495
Ala Arg Lys Asn Met Val Ile Ala Leu Lys Glu Leu Ser Ile Arg Ala
500 505 510
Asp Phe Arg Ser Thr Val Glu Tyr Ile Ile Arg Leu Leu Glu Thr Pro
515 520 525
Asp Phe Glu Glu Asn Thr Ile Asn Thr Gly Trp Leu Asp Met Leu Ile
530 535 540
Ser Lys Lys Leu Thr Ala Glu Arg Pro Asp Thr Met Leu Ala Val Phe
545 550 555 560
Cys Gly Ala Val Thr Lys Ala His Met Ala Ser Leu Asp Cys Phe Gln
565 570 575
Gln Tyr Lys Gln Ser Leu Glu Lys Gly Gln Val Pro Ser Lys Gly Ser
580 585 590
Leu Lys Thr Val Phe Thr Val Asp Phe Ile Tyr Glu Glu Val Arg Tyr
595 600 605
Asn Phe Thr Val Thr Gln Ser Ala Pro Gly Ile Tyr Thr Leu Tyr Leu
610 615 620
Asn Gly Thr Lys Thr Gln Val Gly Ile Arg Asp Leu Ser Asp Gly Gly
625 630 635 640
Leu Leu Ile Ser Ile Asp Gly Lys Ser His Thr Thr Tyr Ser Arg Asp
645 650 655
Glu Val Gln Ala Thr Arg Met Met Val Asp Gly Lys Thr Cys Leu Leu
660 665 670
Glu Lys Glu Ser Asp Pro Thr Gln Leu Arg Ser Pro Ser Pro Gly Lys
675 680 685
Leu Val Asn Leu Leu Val Glu Asn Gly Asp His Leu Asn Ala Gly Asp
690 695 700
Ala Tyr Ala Glu Ile Glu Val Met Lys Met Tyr Met Pro Leu Ile Ala
705 710 715 720
Thr Glu Asp Gly His Val Gln Phe Ile Lys Gln Ala Gly Ala Thr Leu
725 730 735
Glu Ala Gly Asp Ile Ile Gly Ile Leu Ser Leu Asp Asp Pro Ser Arg
740 745 750
Val Lys His Ala Leu Pro Phe Asn Gly Thr Val Pro Ala Phe Gly Ala
755 760 765
Pro His Ile Thr Gly Asp Lys Pro Val Gln Arg Phe Asn Ala Thr Lys
770 775 780
Leu Thr Leu Gln His Ile Leu Gln Gly Tyr Asp Asn Gln Ala Leu Val
785 790 795 800
Gln Thr Val Val Lys Asp Phe Ala Asp Ile Leu Asn Asn Pro Asp Leu
805 810 815
Pro Tyr Ser Glu Leu Asn Ser Val Leu Ser Ala Leu Ser Gly Arg Ile
820 825 830
Pro Gln Arg Leu Glu Ala Ser Ile His Lys Leu Ala Asp Glu Ser Lys
835 840 845
Ala Ala Asn Gln Glu Phe Pro Ala Ala Gln Phe Glu Lys Leu Val Glu
850 855 860
Asp Phe Ala Arg Glu His Ile Thr Leu Gln Ser Glu Ala Thr Ala Tyr
865 870 875 880
Lys Asn Ser Val Ala Pro Leu Ser Ser Ile Phe Ala Arg Tyr Arg Asn
885 890 895
Gly Leu Thr Glu His Ala Tyr Ser Asn Tyr Val Glu Leu Met Glu Ala
900 905 910
Tyr Tyr Asp Val Glu Ile Leu Phe Asn Gln Gln Arg Glu Glu Glu Val
915 920 925
Ile Leu Ser Leu Arg Asp Gln His Lys Asp Asp Leu Asp Lys Val Leu
930 935 940
Ala Val Thr Leu Ser His Ala Lys Val Asn Ile Lys Asn Asn Val Ile
945 950 955 960
Leu Met Leu Leu Asp Leu Ile Asn Pro Val Ser Thr Gly Ser Ala Leu
965 970 975
Asp Lys Tyr Phe Thr Pro Ile Leu Lys Arg Leu Ser Glu Ile Glu Ser
980 985 990
Arg Ala Thr Gln Lys Val Thr Leu Lys Ala Arg Glu Leu Leu Ile Leu
995 1000 1005
Cys Gln Leu Pro Ser Tyr Glu Glu Arg Gln Ala Gln Met Tyr Gln
1010 1015 1020
Ile Leu Lys Asn Ser Val Thr Glu Ser Val Tyr Gly Gly Gly Ser
1025 1030 1035
Glu Tyr Arg Thr Pro Ser Tyr Asp Ala Phe Lys Asp Leu Ile Asp
1040 1045 1050
Thr Lys Phe Asn Val Phe Asp Val Leu Pro His Phe Phe Tyr His
1055 1060 1065
Ala Asp Pro Tyr Ile Ala Leu Ala Ala Ile Glu Val Tyr Cys Arg
1070 1075 1080
Arg Ser Tyr His Ala Tyr Lys Ile Leu Asp Val Ala Tyr Asn Leu
1085 1090 1095
Glu His Lys Pro Tyr Val Val Ala Trp Lys Phe Leu Leu Gln Thr
1100 1105 1110
Ala Ala Asn Gly Ile Asp Ser Asn Lys Arg Ile Ala Ser Tyr Ser
1115 1120 1125
Asp Leu Thr Phe Leu Leu Asn Lys Thr Glu Glu Glu Pro Ile Arg
1130 1135 1140
Thr Gly Ala Met Thr Ala Cys Asn Ser Leu Ala Asp Leu Gln Ala
1145 1150 1155
Glu Leu Pro Arg Ile Leu Thr Ala Phe Glu Glu Glu Pro Leu Pro
1160 1165 1170
Pro Met Leu Gln Arg Asn Ala Ala Pro Lys Glu Glu Arg Met Glu
1175 1180 1185
Asn Ile Leu Asn Ile Ala Val Arg Ala Asp Glu Asp Met Asp Asp
1190 1195 1200
Thr Ala Phe Arg Thr Lys Ile Cys Glu Met Ile Thr Ala Asn Ala
1205 1210 1215
Asp Ala Phe Arg Gln Ala His Leu Arg Arg Leu Ser Val Val Val
1220 1225 1230
Cys Arg Asp Asn Gln Trp Pro Asp Tyr Tyr Thr Phe Arg Glu Arg
1235 1240 1245
Glu Asn Tyr Gln Glu Asp Glu Thr Ile Arg His Ile Glu Pro Ala
1250 1255 1260
Met Ala Tyr Gln Leu Glu Leu Ala Arg Leu Ser Asn Phe Asp Ile
1265 1270 1275
Lys Pro Cys Phe Ile Glu Asn Arg Gln Met His Val Tyr Tyr Ala
1280 1285 1290
Val Ala Lys Glu Asn Pro Ser Asp Cys Arg Phe Phe Ile Arg Ala
1295 1300 1305
Leu Val Arg Pro Gly Arg Val Lys Ser Ser Met Arg Thr Ala Asp
1310 1315 1320
Tyr Leu Ile Ser Glu Ser Asp Arg Leu Leu Thr Asp Ile Leu Asp
1325 1330 1335
Thr Leu Glu Ile Val Ser His Glu Tyr Lys Asn Ser Asp Cys Asn
1340 1345 1350
His Leu Phe Ile Asn Phe Ile Pro Thr Phe Ala Ile Glu Ala Asp
1355 1360 1365
Asp Val Glu His Ala Leu Lys Asp Phe Val Asp Arg His Gly Lys
1370 1375 1380
Arg Leu Trp Lys Leu Arg Val Thr Gly Ala Glu Ile Arg Phe Asn
1385 1390 1395
Val Gln Ser Lys Lys Pro Asp Ala Pro Ile Ile Pro Met Arg Phe
1400 1405 1410
Thr Val Asp Asn Val Ser Gly Phe Ile Leu Lys Val Glu Val Tyr
1415 1420 1425
Gln Glu Val Lys Thr Glu Lys Ser Gly Trp Ile Leu Lys Ser Val
1430 1435 1440
Asn Lys Ile Pro Gly Ala Met His Met Gln Pro Leu Ser Thr Pro
1445 1450 1455
Tyr Pro Thr Lys Glu Trp Leu Gln Pro Arg Arg Tyr Lys Ala His
1460 1465 1470
Leu Met Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg
1475 1480 1485
Gln Ser Val Gln Asn Gln Trp Thr Gln Ala Ile Lys Arg Asn Pro
1490 1495 1500
Leu Leu Lys Gln Pro Ser His Leu Val Glu Ala Lys Glu Leu Val
1505 1510 1515
Leu Asp Glu Asp Asp Val Leu Gln Glu Ile Asp Arg Ala Pro Gly
1520 1525 1530
Thr Asn Thr Val Gly Met Val Ala Trp Ile Met Thr Ile Arg Thr
1535 1540 1545
Pro Glu Tyr Pro Ser Gly Arg Arg Ile Ile Ala Ile Ala Asn Asp
1550 1555 1560
Ile Thr Phe Lys Ile Gly Ser Phe Gly Val Ala Glu Asp Gln Val
1565 1570 1575
Phe Tyr Lys Ala Ser Glu Leu Ala Arg Ala Leu Gly Ile Pro Arg
1580 1585 1590
Ile Tyr Leu Ser Ala Asn Ser Gly Ala Arg Ile Gly Leu Ala Glu
1595 1600 1605
Glu Leu Ile Ser Gln Phe Arg Ala Ala Trp Lys Asp Ala Ser Asn
1610 1615 1620
Pro Thr Ala Gly Phe Asn Tyr Leu Tyr Leu Thr Pro Ala Glu Tyr
1625 1630 1635
Asp Thr Leu Val Gln Gln Gly Asp Ala Lys Ser Val Leu Val Glu
1640 1645 1650
Glu Ile Gln Asp Glu Gly Glu Thr Arg Leu Arg Ile Thr Asp Val
1655 1660 1665
Ile Gly His Thr Asp Gly Leu Gly Val Glu Asn Leu Lys Gly Ser
1670 1675 1680
Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr Asp Asp Ile Phe
1685 1690 1695
Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr
1700 1705 1710
Leu Val Arg Leu Gly Gln Arg Thr Val Gln Asn Glu Gly Gln Pro
1715 1720 1725
Ile Ile Leu Thr Gly Ala Pro Ala Leu Asn Lys Val Leu Gly Arg
1730 1735 1740
Glu Val Tyr Thr Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile Met
1745 1750 1755
Tyr Lys Asn Gly Val Ser His Leu Thr Ala Glu Asn Asp Leu Glu
1760 1765 1770
Gly Ile Ala Lys Ile Val Gln Trp Leu Ser Phe Val Pro Asp Val
1775 1780 1785
Arg Asn Ala Pro Val Ser Met Arg Leu Gly Val Asp Pro Ile Asp
1790 1795 1800
Arg Asp Ile Glu Tyr Thr Pro Pro Lys Gly Pro Ser Asp Pro Arg
1805 1810 1815
Phe Phe Leu Ala Gly Lys Ser Glu Asn Gly Lys Trp Leu Ser Gly
1820 1825 1830
Phe Phe Asp Gln Asp Ser Phe Ile Glu Thr Leu Ser Gly Trp Ala
1835 1840 1845
Arg Thr Val Val Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Met
1850 1855 1860
Gly Val Val Ser Val Glu Thr Arg Thr Val Glu Asn Ile Val Pro
1865 1870 1875
Ala Asp Pro Ala Asn Ser Asp Ser Thr Glu Gln Val Phe Met Glu
1880 1885 1890
Ala Gly Gly Val Trp Phe Pro Asn Ser Ala Tyr Lys Thr Ala Gln
1895 1900 1905
Ala Ile Asn Asp Phe Asn Lys Gly Glu Gln Leu Pro Leu Met Ile
1910 1915 1920
Phe Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Tyr
1925 1930 1935
Asn Glu Val Leu Lys Tyr Gly Ala Gln Ile Val Asp Ala Leu Ser
1940 1945 1950
Asn Tyr Lys Gln Pro Val Phe Val Tyr Ile Ile Pro Asn Gly Glu
1955 1960 1965
Leu Arg Gly Gly Ala Trp Val Val Val Asp Pro Thr Ile Asn Lys
1970 1975 1980
Asp Met Met Glu Met Tyr Ala Asp Asn Asn Ala Arg Gly Gly Val
1985 1990 1995
Leu Glu Pro Glu Gly Ile Val Glu Ile Lys Tyr Arg Lys Pro Ala
2000 2005 2010
Leu Leu Ala Thr Met Glu Arg Leu Asp Ala Thr Tyr Ala Ser Leu
2015 2020 2025
Lys Lys Gln Leu Ala Glu Glu Gly Lys Ser Asp Glu Glu Lys Ala
2030 2035 2040
Ala Leu Lys Val Gln Ile Glu Ala Arg Glu Gln Glu Leu Leu Pro
2045 2050 2055
Val Tyr Gln Gln Ile Ser Ile Gln Phe Ala Asp Leu His Asp Arg
2060 2065 2070
Ala Gly Arg Met Lys Ala Lys Gly Val Ile Arg Lys Ala Leu Asp
2075 2080 2085
Trp Arg Arg Ala Arg His Tyr Phe Tyr Trp Arg Val Arg Arg Arg
2090 2095 2100
Leu Cys Glu Glu Tyr Thr Phe Arg Lys Ile Val Ala Ala Thr Ser
2105 2110 2115
Ala Ala Pro Met Ser Arg Glu Gln Met Leu Asp Leu Val Lys Gln
2120 2125 2130
Trp Phe Thr Ser Asp Asn Glu Thr Ala Ser Phe Glu Asp Ala Asp
2135 2140 2145
Glu Leu Val Ser Glu Trp Phe Glu Lys Arg Ala Ser Val Ile Asp
2150 2155 2160
Gln Arg Ile Ser Lys Leu Lys Ser Asp Ala Thr Lys Glu Gln Val
2165 2170 2175
Val Ser Leu Gly Asn Ala Asp Gln Glu Ala Val Ile Glu Gly Phe
2180 2185 2190
Thr Gln Leu Ile Glu Asn Leu Ser Glu Asp Gln Arg Ala Glu Ile
2195 2200 2205
Leu Arg Lys Leu Asn Ser Arg Phe
2210 2215
<210> 104
<211> 2233
<212> PRT
<213> ACC1sc
<400> 104
Met Ser Glu Glu Ser Leu Phe Glu Ser Ser Pro Gln Lys Met Glu Tyr
1 5 10 15
Glu Ile Thr Asn Tyr Ser Glu Arg His Thr Glu Leu Pro Gly His Phe
20 25 30
Ile Gly Leu Asn Thr Val Asp Lys Leu Glu Glu Ser Pro Leu Arg Asp
35 40 45
Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile
50 55 60
Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys
65 70 75 80
Trp Ala Tyr Glu Thr Phe Gly Asp Asp Arg Thr Val Gln Phe Val Ala
85 90 95
Met Ala Thr Pro Glu Asp Leu Glu Ala Asn Ala Glu Tyr Ile Arg Met
100 105 110
Ala Asp Gln Tyr Ile Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr
115 120 125
Ala Asn Val Asp Leu Ile Val Asp Ile Ala Glu Arg Ala Asp Val Asp
130 135 140
Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro Leu Leu Pro
145 150 155 160
Glu Lys Leu Ser Gln Ser Lys Arg Lys Val Ile Phe Ile Gly Pro Pro
165 170 175
Gly Asn Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val
180 185 190
Ala Gln Ser Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val
195 200 205
Asp Thr Val His Val Asp Glu Lys Thr Gly Leu Val Ser Val Asp Asp
210 215 220
Asp Ile Tyr Gln Lys Gly Cys Cys Thr Ser Pro Glu Asp Gly Leu Gln
225 230 235 240
Lys Ala Lys Arg Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly
245 250 255
Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile
260 265 270
Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe
275 280 285
Ile Met Lys Leu Ala Gly Arg Ala Arg His Leu Glu Val Gln Leu Leu
290 295 300
Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser
305 310 315 320
Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile
325 330 335
Ala Lys Ala Glu Thr Phe His Glu Met Glu Lys Ala Ala Val Arg Leu
340 345 350
Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr
355 360 365
Ser His Asp Asp Gly Lys Phe Tyr Phe Leu Glu Leu Asn Pro Arg Leu
370 375 380
Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro
385 390 395 400
Ala Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser
405 410 415
Asp Ile Arg Thr Leu Tyr Gly Met Asn Pro His Ser Ala Ser Glu Ile
420 425 430
Asp Phe Glu Phe Lys Thr Gln Asp Ala Thr Lys Lys Gln Arg Arg Pro
435 440 445
Ile Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro
450 455 460
Asn Asp Gly Phe Lys Pro Ser Gly Gly Thr Leu His Glu Leu Asn Phe
465 470 475 480
Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly
485 490 495
Asn Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe
500 505 510
Gly Glu Asn Arg Gln Ala Ser Arg Lys His Met Val Val Ala Leu Lys
515 520 525
Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile
530 535 540
Lys Leu Leu Glu Thr Glu Asp Phe Glu Asp Asn Thr Ile Thr Thr Gly
545 550 555 560
Trp Leu Asp Asp Leu Ile Thr His Lys Met Thr Ala Glu Lys Pro Asp
565 570 575
Pro Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Leu Ala
580 585 590
Ser Glu Glu Ala Arg His Lys Tyr Ile Glu Ser Leu Gln Lys Gly Gln
595 600 605
Val Leu Ser Lys Asp Leu Leu Gln Thr Met Phe Pro Val Asp Phe Ile
610 615 620
His Glu Gly Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Gly Asn Asp
625 630 635 640
Arg Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Asp Ile Ile Leu Arg
645 650 655
Gln Leu Ser Asp Gly Gly Leu Leu Ile Ala Ile Gly Gly Lys Ser His
660 665 670
Thr Ile Tyr Trp Lys Glu Glu Val Ala Ala Thr Arg Leu Ser Val Asp
675 680 685
Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg
690 695 700
Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Glu
705 710 715 720
His Ile Ile Lys Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met
725 730 735
Gln Met Pro Leu Val Ser Gln Glu Asn Gly Ile Val Gln Leu Leu Lys
740 745 750
Gln Pro Gly Ser Thr Ile Val Ala Gly Asp Ile Met Ala Ile Met Thr
755 760 765
Leu Asp Asp Pro Ser Lys Val Lys His Ala Leu Pro Phe Glu Gly Met
770 775 780
Leu Pro Asp Phe Gly Ser Pro Val Ile Glu Gly Thr Lys Pro Ala Tyr
785 790 795 800
Lys Phe Lys Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr
805 810 815
Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val
820 825 830
Leu Arg Asn Pro Lys Leu Pro Tyr Ser Glu Trp Lys Leu His Ile Ser
835 840 845
Ala Leu His Ser Arg Leu Pro Ala Lys Leu Asp Glu Gln Met Glu Glu
850 855 860
Leu Val Ala Arg Ser Leu Arg Arg Gly Ala Val Phe Pro Ala Arg Gln
865 870 875 880
Leu Ser Lys Leu Ile Asp Met Ala Val Lys Asn Pro Glu Tyr Asn Pro
885 890 895
Asp Lys Leu Leu Gly Ala Val Val Glu Pro Leu Ala Asp Ile Ala His
900 905 910
Lys Tyr Ser Asn Gly Leu Glu Ala His Glu His Ser Ile Phe Val His
915 920 925
Phe Leu Glu Glu Tyr Tyr Glu Val Glu Lys Leu Phe Asn Gly Pro Asn
930 935 940
Val Arg Glu Glu Asn Ile Ile Leu Lys Leu Arg Asp Glu Asn Pro Lys
945 950 955 960
Asp Leu Asp Lys Val Ala Leu Thr Val Leu Ser His Ser Lys Val Ser
965 970 975
Ala Lys Asn Asn Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Leu
980 985 990
Cys Lys Leu Ser Ser Lys Val Ser Ala Ile Phe Ser Thr Pro Leu Gln
995 1000 1005
His Ile Val Glu Leu Glu Ser Lys Ala Thr Ala Lys Val Ala Leu
1010 1015 1020
Gln Ala Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys
1025 1030 1035
Glu Arg Thr Glu Gln Ile Glu His Ile Leu Lys Ser Ser Val Val
1040 1045 1050
Lys Val Ala Tyr Gly Ser Ser Asn Pro Lys Arg Ser Glu Pro Asp
1055 1060 1065
Leu Asn Ile Leu Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe
1070 1075 1080
Asp Val Leu Leu Gln Phe Leu Thr His Gln Asp Pro Val Val Thr
1085 1090 1095
Ala Ala Ala Ala Gln Val Tyr Ile Arg Arg Ala Tyr Arg Ala Tyr
1100 1105 1110
Thr Ile Gly Asp Ile Arg Val His Glu Gly Val Thr Val Pro Ile
1115 1120 1125
Val Glu Trp Lys Phe Gln Leu Pro Ser Ala Ala Phe Ser Thr Phe
1130 1135 1140
Pro Thr Val Lys Ser Lys Met Gly Met Asn Arg Ala Val Ser Val
1145 1150 1155
Ser Asp Leu Ser Tyr Val Ala Asn Ser Gln Ser Ser Pro Leu Arg
1160 1165 1170
Glu Gly Ile Leu Met Ala Val Asp His Leu Asp Asp Val Asp Glu
1175 1180 1185
Ile Leu Ser Gln Ser Leu Glu Val Ile Pro Arg His Gln Ser Ser
1190 1195 1200
Ser Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ala Ser Leu
1205 1210 1215
Ser Asn Val Ala Asn Val Cys Val Ala Ser Thr Glu Gly Phe Glu
1220 1225 1230
Ser Glu Glu Glu Ile Leu Val Arg Leu Arg Glu Ile Leu Asp Leu
1235 1240 1245
Asn Lys Gln Glu Leu Ile Asn Ala Ser Ile Arg Arg Ile Thr Phe
1250 1255 1260
Met Phe Gly Phe Lys Asp Gly Ser Tyr Pro Lys Tyr Tyr Thr Phe
1265 1270 1275
Asn Gly Pro Asn Tyr Asn Glu Asn Glu Thr Ile Arg His Ile Glu
1280 1285 1290
Pro Ala Leu Ala Phe Gln Leu Glu Leu Gly Arg Leu Ser Asn Phe
1295 1300 1305
Asn Ile Lys Pro Ile Phe Thr Asp Asn Arg Asn Ile His Val Tyr
1310 1315 1320
Glu Ala Val Ser Lys Thr Ser Pro Leu Asp Lys Arg Phe Phe Thr
1325 1330 1335
Arg Gly Ile Ile Arg Thr Gly His Ile Arg Asp Asp Ile Ser Ile
1340 1345 1350
Gln Glu Tyr Leu Thr Ser Glu Ala Asn Arg Leu Met Ser Asp Ile
1355 1360 1365
Leu Asp Asn Leu Glu Val Thr Asp Thr Ser Asn Ser Asp Leu Asn
1370 1375 1380
His Ile Phe Ile Asn Phe Ile Ala Val Phe Asp Ile Ser Pro Glu
1385 1390 1395
Asp Val Glu Ala Ala Phe Gly Gly Phe Leu Glu Arg Phe Gly Lys
1400 1405 1410
Arg Leu Leu Arg Leu Arg Val Ser Ser Ala Glu Ile Arg Ile Ile
1415 1420 1425
Ile Lys Asp Pro Gln Thr Gly Ala Pro Val Pro Leu Arg Ala Leu
1430 1435 1440
Ile Asn Asn Val Ser Gly Tyr Val Ile Lys Thr Glu Met Tyr Thr
1445 1450 1455
Glu Val Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu Gly
1460 1465 1470
Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr Pro
1475 1480 1485
Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu Met
1490 1495 1500
Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe Arg Gln Ala
1505 1510 1515
Ser Ser Ser Gln Trp Lys Asn Phe Ser Ala Asp Val Lys Leu Thr
1520 1525 1530
Asp Asp Phe Phe Ile Ser Asn Glu Leu Ile Glu Asp Glu Asn Gly
1535 1540 1545
Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala Asn Ala Ile Gly
1550 1555 1560
Met Val Ala Phe Lys Ile Thr Val Lys Thr Pro Glu Tyr Pro Arg
1565 1570 1575
Gly Arg Gln Phe Val Val Val Ala Asn Asp Ile Thr Phe Lys Ile
1580 1585 1590
Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val Thr
1595 1600 1605
Glu Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala
1610 1615 1620
Asn Ser Gly Ala Arg Ile Gly Met Ala Glu Glu Ile Val Pro Leu
1625 1630 1635
Phe Gln Val Ala Trp Asn Asp Ala Ala Asn Pro Asp Lys Gly Phe
1640 1645 1650
Gln Tyr Leu Tyr Leu Thr Ser Glu Gly Met Glu Thr Leu Lys Lys
1655 1660 1665
Phe Asp Lys Glu Asn Ser Val Leu Thr Glu Arg Thr Val Ile Asn
1670 1675 1680
Gly Glu Glu Arg Phe Val Ile Lys Thr Ile Ile Gly Ser Glu Asp
1685 1690 1695
Gly Leu Gly Val Glu Cys Leu Arg Gly Ser Gly Leu Ile Ala Gly
1700 1705 1710
Ala Thr Ser Arg Ala Tyr His Asp Ile Phe Thr Ile Thr Leu Val
1715 1720 1725
Thr Cys Arg Ser Val Gly Ile Gly Ala Tyr Leu Val Arg Leu Gly
1730 1735 1740
Gln Arg Ala Ile Gln Val Glu Gly Gln Pro Ile Ile Leu Thr Gly
1745 1750 1755
Ala Pro Ala Ile Asn Lys Met Leu Gly Arg Glu Val Tyr Thr Ser
1760 1765 1770
Asn Leu Gln Leu Gly Gly Thr Gln Ile Met Tyr Asn Asn Gly Val
1775 1780 1785
Ser His Leu Thr Ala Val Asp Asp Leu Ala Gly Val Glu Lys Ile
1790 1795 1800
Val Glu Trp Met Ser Tyr Val Pro Ala Lys Arg Asn Met Pro Val
1805 1810 1815
Pro Ile Leu Glu Thr Lys Asp Thr Trp Asp Arg Pro Val Asp Phe
1820 1825 1830
Thr Pro Thr Asn Asp Glu Thr Tyr Asp Val Arg Trp Met Ile Glu
1835 1840 1845
Gly Arg Glu Thr Glu Ser Gly Phe Glu Tyr Gly Leu Phe Asp Lys
1850 1855 1860
Gly Ser Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val
1865 1870 1875
Val Gly Arg Ala Arg Leu Gly Gly Ile Pro Leu Gly Val Ile Gly
1880 1885 1890
Val Glu Thr Arg Thr Val Glu Asn Leu Ile Pro Ala Asp Pro Ala
1895 1900 1905
Asn Pro Asn Ser Ala Glu Thr Leu Ile Gln Glu Pro Gly Gln Val
1910 1915 1920
Trp His Pro Asn Ser Ala Phe Lys Thr Ala Gln Ala Ile Asn Asp
1925 1930 1935
Phe Asn Asn Gly Glu Gln Leu Pro Met Met Ile Leu Ala Asn Trp
1940 1945 1950
Arg Gly Phe Ser Gly Gly Gln Arg Asp Met Phe Asn Glu Val Leu
1955 1960 1965
Lys Tyr Gly Ser Phe Ile Val Asp Ala Leu Val Asp Tyr Lys Gln
1970 1975 1980
Pro Ile Ile Ile Tyr Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly
1985 1990 1995
Ser Trp Val Val Val Asp Pro Thr Ile Asn Ala Asp Gln Met Glu
2000 2005 2010
Met Tyr Ala Asp Val Asn Ala Arg Ala Gly Val Leu Glu Pro Gln
2015 2020 2025
Gly Met Val Gly Ile Lys Phe Arg Arg Glu Lys Leu Leu Asp Thr
2030 2035 2040
Met Asn Arg Leu Asp Asp Lys Tyr Arg Glu Leu Arg Ser Gln Leu
2045 2050 2055
Ser Asn Lys Ser Leu Ala Pro Glu Val His Gln Gln Ile Ser Lys
2060 2065 2070
Gln Leu Ala Asp Arg Glu Arg Glu Leu Leu Pro Ile Tyr Gly Gln
2075 2080 2085
Ile Ser Leu Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg Met
2090 2095 2100
Val Ala Lys Gly Val Ile Ser Lys Glu Leu Glu Trp Thr Glu Ala
2105 2110 2115
Arg Arg Phe Phe Phe Trp Arg Leu Arg Arg Arg Leu Asn Glu Glu
2120 2125 2130
Tyr Leu Ile Lys Arg Leu Ser His Gln Val Gly Glu Ala Ser Arg
2135 2140 2145
Leu Glu Lys Ile Ala Arg Ile Arg Ser Trp Tyr Pro Ala Ser Val
2150 2155 2160
Asp His Glu Asp Asp Arg Gln Val Ala Thr Trp Ile Glu Glu Asn
2165 2170 2175
Tyr Lys Thr Leu Asp Asp Lys Leu Lys Gly Leu Lys Leu Glu Ser
2180 2185 2190
Phe Ala Gln Asp Leu Ala Lys Lys Ile Arg Ser Asp His Asp Asn
2195 2200 2205
Ala Ile Asp Gly Leu Ser Glu Val Ile Lys Met Leu Ser Thr Asp
2210 2215 2220
Asp Lys Glu Lys Leu Leu Lys Thr Leu Lys
2225 2230
<210> 105
<211> 2266
<212> PRT
<213> ACCyl
<400> 105
Met Arg Leu Gln Leu Arg Thr Leu Thr Arg Arg Phe Phe Ser Met Ala
1 5 10 15
Ser Gly Ser Ser Thr Pro Asp Val Ala Pro Leu Val Asp Pro Asn Ile
20 25 30
His Lys Gly Leu Ala Ser His Phe Phe Gly Leu Asn Ser Val His Thr
35 40 45
Ala Lys Pro Ser Lys Val Lys Glu Phe Val Ala Ser His Gly Gly His
50 55 60
Thr Val Ile Asn Lys Val Leu Ile Ala Asn Asn Gly Ile Ala Ala Val
65 70 75 80
Lys Glu Ile Arg Ser Val Arg Lys Trp Ala Tyr Glu Thr Phe Gly Asp
85 90 95
Glu Arg Ala Ile Ser Phe Thr Val Met Ala Thr Pro Glu Asp Leu Ala
100 105 110
Ala Asn Ala Asp Tyr Ile Arg Met Ala Asp Gln Tyr Val Glu Val Pro
115 120 125
Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Glu Leu Ile Val Asp
130 135 140
Val Ala Glu Arg Phe Gly Val Asp Ala Val Trp Ala Gly Trp Gly His
145 150 155 160
Ala Ser Glu Asn Pro Leu Leu Pro Glu Ser Leu Ala Ala Ser Pro Arg
165 170 175
Lys Ile Val Phe Ile Gly Pro Pro Gly Ala Ala Met Arg Ser Leu Gly
180 185 190
Asp Lys Ile Ser Ser Thr Ile Val Ala Gln His Ala Lys Val Pro Cys
195 200 205
Ile Pro Trp Ser Gly Thr Gly Val Asp Glu Val Val Val Asp Lys Ser
210 215 220
Thr Asn Leu Val Ser Val Ser Glu Glu Val Tyr Thr Lys Gly Cys Thr
225 230 235 240
Thr Gly Pro Lys Gln Gly Leu Glu Lys Ala Lys Gln Ile Gly Phe Pro
245 250 255
Val Met Ile Lys Ala Ser Glu Gly Gly Gly Gly Lys Gly Ile Arg Lys
260 265 270
Val Glu Arg Glu Glu Asp Phe Glu Ala Ala Tyr His Gln Val Glu Gly
275 280 285
Glu Ile Pro Gly Ser Pro Ile Phe Ile Met Gln Leu Ala Gly Asn Ala
290 295 300
Arg His Leu Glu Val Gln Leu Leu Ala Asp Gln Tyr Gly Asn Asn Ile
305 310 315 320
Ser Leu Phe Gly Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile
325 330 335
Ile Glu Glu Ala Pro Val Thr Val Ala Gly Gln Gln Thr Phe Thr Ala
340 345 350
Met Glu Lys Ala Ala Val Arg Leu Gly Lys Leu Val Gly Tyr Val Ser
355 360 365
Ala Gly Thr Val Glu Tyr Leu Tyr Ser His Glu Asp Asp Lys Phe Tyr
370 375 380
Phe Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Thr Thr Glu
385 390 395 400
Met Val Thr Gly Val Asn Leu Pro Ala Ala Gln Leu Gln Ile Ala Met
405 410 415
Gly Ile Pro Leu Asp Arg Ile Lys Asp Ile Arg Leu Phe Tyr Gly Val
420 425 430
Asn Pro His Thr Thr Thr Pro Ile Asp Phe Asp Phe Ser Gly Glu Asp
435 440 445
Ala Asp Lys Thr Gln Arg Arg Pro Val Pro Arg Gly His Thr Thr Ala
450 455 460
Cys Arg Ile Thr Ser Glu Asp Pro Gly Glu Gly Phe Lys Pro Ser Gly
465 470 475 480
Gly Thr Met His Glu Leu Asn Phe Arg Ser Ser Ser Asn Val Trp Gly
485 490 495
Tyr Phe Ser Val Gly Asn Gln Gly Gly Ile His Ser Phe Ser Asp Ser
500 505 510
Gln Phe Gly His Ile Phe Ala Phe Gly Glu Asn Arg Ser Ala Ser Arg
515 520 525
Lys His Met Val Val Ala Leu Lys Glu Leu Ser Ile Arg Gly Asp Phe
530 535 540
Arg Thr Thr Val Glu Tyr Leu Ile Lys Leu Leu Glu Thr Pro Asp Phe
545 550 555 560
Glu Asp Asn Thr Ile Thr Thr Gly Trp Leu Asp Glu Leu Ile Ser Asn
565 570 575
Lys Leu Thr Ala Glu Arg Pro Asp Ser Phe Leu Ala Val Val Cys Gly
580 585 590
Ala Ala Thr Lys Ala His Arg Ala Ser Glu Asp Ser Ile Ala Thr Tyr
595 600 605
Met Ala Ser Leu Glu Lys Gly Gln Val Pro Ala Arg Asp Ile Leu Lys
610 615 620
Thr Leu Phe Pro Val Asp Phe Ile Tyr Glu Gly Gln Arg Tyr Lys Phe
625 630 635 640
Thr Ala Thr Arg Ser Ser Glu Asp Ser Tyr Thr Leu Phe Ile Asn Gly
645 650 655
Ser Arg Cys Asp Ile Gly Val Arg Pro Leu Ser Asp Gly Gly Ile Leu
660 665 670
Cys Leu Val Gly Gly Arg Ser His Asn Val Tyr Trp Lys Glu Glu Val
675 680 685
Gly Ala Thr Arg Leu Ser Val Asp Ser Lys Thr Cys Leu Leu Glu Val
690 695 700
Glu Asn Asp Pro Thr Gln Leu Arg Ser Pro Ser Pro Gly Lys Leu Val
705 710 715 720
Lys Phe Leu Val Glu Asn Gly Asp His Val Arg Ala Asn Gln Pro Tyr
725 730 735
Ala Glu Ile Glu Val Met Lys Met Tyr Met Thr Leu Thr Ala Gln Glu
740 745 750
Asp Gly Ile Val Gln Leu Met Lys Gln Pro Gly Ser Thr Ile Glu Ala
755 760 765
Gly Asp Ile Leu Gly Ile Leu Ala Leu Asp Asp Pro Ser Lys Val Lys
770 775 780
His Ala Lys Pro Phe Glu Gly Gln Leu Pro Glu Leu Gly Pro Pro Thr
785 790 795 800
Leu Ser Gly Asn Lys Pro His Gln Arg Tyr Glu His Cys Gln Asn Val
805 810 815
Leu His Asn Ile Leu Leu Gly Phe Asp Asn Gln Val Val Met Lys Ser
820 825 830
Thr Leu Gln Glu Met Val Gly Leu Leu Arg Asn Pro Glu Leu Pro Tyr
835 840 845
Leu Gln Trp Ala His Gln Val Ser Ser Leu His Thr Arg Met Ser Ala
850 855 860
Lys Leu Asp Ala Thr Leu Ala Gly Leu Ile Asp Lys Ala Lys Gln Arg
865 870 875 880
Gly Gly Glu Phe Pro Ala Lys Gln Leu Leu Arg Ala Leu Glu Lys Glu
885 890 895
Ala Ser Ser Gly Glu Val Asp Ala Leu Phe Gln Gln Thr Leu Ala Pro
900 905 910
Leu Phe Asp Leu Ala Arg Glu Tyr Gln Asp Gly Leu Ala Ile His Glu
915 920 925
Leu Gln Val Ala Ala Gly Leu Leu Gln Ala Tyr Tyr Asp Ser Glu Ala
930 935 940
Arg Phe Cys Gly Pro Asn Val Arg Asp Glu Asp Val Ile Leu Lys Leu
945 950 955 960
Arg Glu Glu Asn Arg Asp Ser Leu Arg Lys Val Val Met Ala Gln Leu
965 970 975
Ser His Ser Arg Val Gly Ala Lys Asn Asn Leu Val Leu Ala Leu Leu
980 985 990
Asp Glu Tyr Lys Val Ala Asp Gln Ala Gly Thr Asp Ser Pro Ala Ser
995 1000 1005
Asn Val His Val Ala Lys Tyr Leu Arg Pro Val Leu Arg Lys Ile
1010 1015 1020
Val Glu Leu Glu Ser Arg Ala Ser Ala Lys Val Ser Leu Lys Ala
1025 1030 1035
Arg Glu Ile Leu Ile Gln Cys Ala Leu Pro Ser Leu Lys Glu Arg
1040 1045 1050
Thr Asp Gln Leu Glu His Ile Leu Arg Ser Ser Val Val Glu Ser
1055 1060 1065
Arg Tyr Gly Glu Val Gly Leu Glu His Arg Thr Pro Arg Ala Asp
1070 1075 1080
Ile Leu Lys Glu Val Val Asp Ser Lys Tyr Ile Val Phe Asp Val
1085 1090 1095
Leu Ala Gln Phe Phe Ala His Asp Asp Pro Trp Ile Val Leu Ala
1100 1105 1110
Ala Leu Glu Leu Tyr Ile Arg Arg Ala Cys Lys Ala Tyr Ser Ile
1115 1120 1125
Leu Asp Ile Asn Tyr His Gln Asp Ser Asp Leu Pro Pro Val Ile
1130 1135 1140
Ser Trp Arg Phe Arg Leu Pro Thr Met Ser Ser Ala Leu Tyr Asn
1145 1150 1155
Ser Val Val Ser Ser Gly Ser Lys Thr Pro Thr Ser Pro Ser Val
1160 1165 1170
Ser Arg Ala Asp Ser Val Ser Asp Phe Ser Tyr Thr Val Glu Arg
1175 1180 1185
Asp Ser Ala Pro Ala Arg Thr Gly Ala Ile Val Ala Val Pro His
1190 1195 1200
Leu Asp Asp Leu Glu Asp Ala Leu Thr Arg Val Leu Glu Asn Leu
1205 1210 1215
Pro Lys Arg Gly Ala Gly Leu Ala Ile Ser Val Gly Ala Ser Asn
1220 1225 1230
Lys Ser Ala Ala Ala Ser Ala Arg Asp Ala Ala Ala Ala Ala Ala
1235 1240 1245
Ser Ser Val Asp Thr Gly Leu Ser Asn Ile Cys Asn Val Met Ile
1250 1255 1260
Gly Arg Val Asp Glu Ser Asp Asp Asp Asp Thr Leu Ile Ala Arg
1265 1270 1275
Ile Ser Gln Val Ile Glu Asp Phe Lys Glu Asp Phe Glu Ala Cys
1280 1285 1290
Ser Leu Arg Arg Ile Thr Phe Ser Phe Gly Asn Ser Arg Gly Thr
1295 1300 1305
Tyr Pro Lys Tyr Phe Thr Phe Arg Gly Pro Ala Tyr Glu Glu Asp
1310 1315 1320
Pro Thr Ile Arg His Ile Glu Pro Ala Leu Ala Phe Gln Leu Glu
1325 1330 1335
Leu Ala Arg Leu Ser Asn Phe Asp Ile Lys Pro Val His Thr Asp
1340 1345 1350
Asn Arg Asn Ile His Val Tyr Glu Ala Thr Gly Lys Asn Ala Ala
1355 1360 1365
Ser Asp Lys Arg Phe Phe Thr Arg Gly Ile Val Arg Pro Gly Arg
1370 1375 1380
Leu Arg Glu Asn Ile Pro Thr Ser Glu Tyr Leu Ile Ser Glu Ala
1385 1390 1395
Asp Arg Leu Met Ser Asp Ile Leu Asp Ala Leu Glu Val Ile Gly
1400 1405 1410
Thr Thr Asn Ser Asp Leu Asn His Ile Phe Ile Asn Phe Ser Ala
1415 1420 1425
Val Phe Ala Leu Lys Pro Glu Glu Val Glu Ala Ala Phe Gly Gly
1430 1435 1440
Phe Leu Glu Arg Phe Gly Arg Arg Leu Trp Arg Leu Arg Val Thr
1445 1450 1455
Gly Ala Glu Ile Arg Met Met Val Ser Asp Pro Glu Thr Gly Ser
1460 1465 1470
Ala Phe Pro Leu Arg Ala Met Ile Asn Asn Val Ser Gly Tyr Val
1475 1480 1485
Val Gln Ser Glu Leu Tyr Ala Glu Ala Lys Asn Asp Lys Gly Gln
1490 1495 1500
Trp Ile Phe Lys Ser Leu Gly Lys Pro Gly Ser Met His Met Arg
1505 1510 1515
Ser Ile Asn Thr Pro Tyr Pro Thr Lys Glu Trp Leu Gln Pro Lys
1520 1525 1530
Arg Tyr Lys Ala His Leu Met Gly Thr Thr Tyr Cys Tyr Asp Phe
1535 1540 1545
Pro Glu Leu Phe Arg Gln Ser Ile Glu Ser Asp Trp Lys Lys Tyr
1550 1555 1560
Asp Gly Lys Ala Pro Asp Asp Leu Met Thr Cys Asn Glu Leu Ile
1565 1570 1575
Leu Asp Glu Asp Ser Gly Glu Leu Gln Glu Val Asn Arg Glu Pro
1580 1585 1590
Gly Ala Asn Asn Val Gly Met Val Ala Trp Lys Phe Glu Ala Lys
1595 1600 1605
Thr Pro Glu Tyr Pro Arg Gly Arg Ser Phe Ile Val Val Ala Asn
1610 1615 1620
Asp Ile Thr Phe Gln Ile Gly Ser Phe Gly Pro Ala Glu Asp Gln
1625 1630 1635
Phe Phe Phe Lys Val Thr Glu Leu Ala Arg Lys Leu Gly Ile Pro
1640 1645 1650
Arg Ile Tyr Leu Ser Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala
1655 1660 1665
Asp Glu Leu Val Gly Lys Tyr Lys Val Ala Trp Asn Asp Glu Thr
1670 1675 1680
Asp Pro Ser Lys Gly Phe Lys Tyr Leu Tyr Phe Thr Pro Glu Ser
1685 1690 1695
Leu Ala Thr Leu Lys Pro Asp Thr Val Val Thr Thr Glu Ile Glu
1700 1705 1710
Glu Glu Gly Pro Asn Gly Val Glu Lys Arg His Val Ile Asp Tyr
1715 1720 1725
Ile Val Gly Glu Lys Asp Gly Leu Gly Val Glu Cys Leu Arg Gly
1730 1735 1740
Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr Lys Asp Ile
1745 1750 1755
Phe Thr Leu Thr Leu Val Thr Cys Arg Ser Val Gly Ile Gly Ala
1760 1765 1770
Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Ile Glu Gly Gln
1775 1780 1785
Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys Leu Leu Gly
1790 1795 1800
Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile
1805 1810 1815
Met Tyr Asn Asn Gly Val Ser His Leu Thr Ala Arg Asp Asp Leu
1820 1825 1830
Asn Gly Val His Lys Ile Met Gln Trp Leu Ser Tyr Ile Pro Ala
1835 1840 1845
Ser Arg Gly Leu Pro Val Pro Val Leu Pro His Lys Thr Asp Val
1850 1855 1860
Trp Asp Arg Asp Val Thr Phe Gln Pro Val Arg Gly Glu Gln Tyr
1865 1870 1875
Asp Val Arg Trp Leu Ile Ser Gly Arg Thr Leu Glu Asp Gly Ala
1880 1885 1890
Phe Glu Ser Gly Leu Phe Asp Lys Asp Ser Phe Gln Glu Thr Leu
1895 1900 1905
Ser Gly Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg Leu Gly
1910 1915 1920
Gly Ile Pro Phe Gly Val Ile Gly Val Glu Thr Ala Thr Val Asp
1925 1930 1935
Asn Thr Thr Pro Ala Asp Pro Ala Asn Pro Asp Ser Ile Glu Met
1940 1945 1950
Ser Thr Ser Glu Ala Gly Gln Val Trp Tyr Pro Asn Ser Ala Phe
1955 1960 1965
Lys Thr Ser Gln Ala Ile Asn Asp Phe Asn His Gly Glu Ala Leu
1970 1975 1980
Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln
1985 1990 1995
Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe Ile Val
2000 2005 2010
Asp Ala Leu Val Asp Tyr Lys Gln Pro Ile Met Val Tyr Ile Pro
2015 2020 2025
Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp Val Val Val Asp Pro
2030 2035 2040
Thr Ile Asn Ser Asp Met Met Glu Met Tyr Ala Asp Val Glu Ser
2045 2050 2055
Arg Gly Gly Val Leu Glu Pro Glu Gly Met Val Gly Ile Lys Tyr
2060 2065 2070
Arg Arg Asp Lys Leu Leu Asp Thr Met Ala Arg Leu Asp Pro Glu
2075 2080 2085
Tyr Ser Ser Leu Lys Lys Gln Leu Glu Glu Ser Pro Asp Ser Glu
2090 2095 2100
Glu Leu Lys Val Lys Leu Ser Val Arg Glu Lys Ser Leu Met Pro
2105 2110 2115
Ile Tyr Gln Gln Ile Ser Val Gln Phe Ala Asp Leu His Asp Arg
2120 2125 2130
Ala Gly Arg Met Glu Ala Lys Gly Val Ile Arg Glu Ala Leu Val
2135 2140 2145
Trp Lys Asp Ala Arg Arg Phe Phe Phe Trp Arg Ile Arg Arg Arg
2150 2155 2160
Leu Val Glu Glu Tyr Leu Ile Thr Lys Ile Asn Ser Ile Leu Pro
2165 2170 2175
Ser Cys Thr Arg Leu Glu Cys Leu Ala Arg Ile Lys Ser Trp Lys
2180 2185 2190
Pro Ala Thr Leu Asp Gln Gly Ser Asp Arg Gly Val Ala Glu Trp
2195 2200 2205
Phe Asp Glu Asn Ser Asp Ala Val Ser Ala Arg Leu Ser Glu Leu
2210 2215 2220
Lys Lys Asp Ala Ser Ala Gln Ser Phe Ala Ser Gln Leu Arg Lys
2225 2230 2235
Asp Arg Gln Gly Thr Leu Gln Gly Met Lys Gln Ala Leu Ala Ser
2240 2245 2250
Leu Ser Glu Ala Glu Arg Ala Glu Leu Leu Lys Gly Leu
2255 2260 2265
<210> 106
<211> 2243
<212> PRT
<213> ACC1ch
<400> 106
Met Ser Glu Glu Asn Leu Phe Glu Asp Ala Ala Ala Lys Arg Gln Tyr
1 5 10 15
Glu Ile Thr Asp Tyr Ser Thr Lys His Lys Thr Leu Ala Ser His Phe
20 25 30
Val Gly Leu Asn Thr Leu Glu Arg Val Glu Glu Ser Pro Leu Lys Asp
35 40 45
Phe Val Lys Ser His Gly Gly His Thr Val Ile Ser Lys Ile Leu Ile
50 55 60
Ala Asn Asn Gly Ile Ala Ala Val Lys Glu Ile Arg Ser Val Arg Lys
65 70 75 80
Trp Ala Tyr Glu Thr Phe Gly Asp Glu Arg Thr Val Gln Phe Val Ala
85 90 95
Met Ala Thr Pro Glu Asp Leu Gln Ala Asn Ser Glu Tyr Ile Arg Met
100 105 110
Ala Asp Gln Tyr Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr
115 120 125
Ala Asn Val Asp Leu Ile Val Asp Val Ala Glu Arg Thr Asp Val Asp
130 135 140
Ala Val Trp Ala Gly Trp Gly His Ala Ser Glu Asn Pro His Leu Pro
145 150 155 160
Glu Lys Leu Ala Ala Ser Lys Arg Lys Ile Ile Phe Ile Gly Pro Pro
165 170 175
Gly Ser Ala Met Arg Ser Leu Gly Asp Lys Ile Ser Ser Thr Ile Val
180 185 190
Ala Gln His Ala Lys Val Pro Cys Ile Pro Trp Ser Gly Thr Gly Val
195 200 205
Asp Thr Val His Leu Asp Ser Glu Thr Gly Leu Val Ser Val Asp Asp
210 215 220
Asp Ile Tyr Gln Lys Gly Cys Cys Ser Ser Pro Glu Asp Gly Leu Ala
225 230 235 240
Lys Ala Lys Lys Ile Gly Phe Pro Val Met Ile Lys Ala Ser Glu Gly
245 250 255
Gly Gly Gly Lys Gly Ile Arg Gln Val Glu Arg Glu Glu Asp Phe Ile
260 265 270
Ala Leu Tyr His Gln Ala Ala Asn Glu Ile Pro Gly Ser Pro Ile Phe
275 280 285
Ile Met Lys Leu Ala Gly Lys Ala Arg His Leu Glu Val Gln Leu Leu
290 295 300
Ala Asp Gln Tyr Gly Thr Asn Ile Ser Leu Phe Gly Arg Asp Cys Ser
305 310 315 320
Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Ala Pro Val Thr Ile
325 330 335
Ala Ser Asn Glu Thr Phe Glu Lys Met Ala Ser Ala Ala Val Arg Leu
340 345 350
Gly Lys Leu Val Gly Tyr Val Ser Ala Gly Thr Val Glu Tyr Leu Tyr
355 360 365
Ser Ser Glu Glu Lys Lys Tyr Tyr Phe Leu Glu Leu Asn Pro Arg Leu
370 375 380
Gln Val Glu His Pro Thr Thr Glu Met Val Ser Gly Val Asn Leu Pro
385 390 395 400
Ser Ala Gln Leu Gln Ile Ala Met Gly Ile Pro Met His Arg Ile Ser
405 410 415
Asp Ile Arg Val Leu Tyr Gly Met Asn Pro His Gly Ala Thr Glu Ile
420 425 430
Asp Phe Glu Phe Lys Thr Glu Glu Ala Lys Lys Thr Gln Arg Lys Pro
435 440 445
Val Pro Lys Gly His Cys Thr Ala Cys Arg Ile Thr Ser Glu Asp Pro
450 455 460
Asn Glu Gly Phe Lys Pro Ser Gly Gly Ser Leu His Glu Leu Asn Phe
465 470 475 480
Arg Ser Ser Ser Asn Val Trp Gly Tyr Phe Ser Val Gly Asn Asn Gly
485 490 495
Gly Ile His Ser Phe Ser Asp Ser Gln Phe Gly His Ile Phe Ala Phe
500 505 510
Gly Glu Asn Arg Gln Met Ser Arg Lys His Met Val Val Ala Leu Lys
515 520 525
Glu Leu Ser Ile Arg Gly Asp Phe Arg Thr Thr Val Glu Tyr Leu Ile
530 535 540
Lys Leu Leu Glu Thr Glu Asp Phe Glu Glu Asn Thr Ile Thr Thr Gly
545 550 555 560
Trp Leu Asp Asp Leu Ile Ser His Lys Met Thr Ala Gln Lys Pro Asp
565 570 575
Ala Thr Leu Ala Val Ile Cys Gly Ala Ala Thr Lys Ala Phe Ile Ala
580 585 590
Ser Glu Ser Ala Arg Lys Glu Tyr Ile Asn Gln Leu Lys Arg Gly Gln
595 600 605
Val Pro Pro Lys Gly Leu Leu Glu Thr Met Phe Pro Ile Glu Phe Ile
610 615 620
His Glu Asn Lys Arg Tyr Lys Phe Thr Val Ala Lys Ser Ala Asp Asp
625 630 635 640
Ser Tyr Thr Leu Phe Ile Asn Gly Ser Lys Cys Glu Val Arg Ala Val
645 650 655
Lys Leu Ser Asp Gly Gly Leu Leu Ile Ser Val Gly Gly Gln Ser His
660 665 670
Thr Ile Tyr Trp Lys Glu Glu Ala Gln Ala Thr Arg Leu Ser Ile Asp
675 680 685
Ser Met Thr Thr Leu Leu Glu Val Glu Asn Asp Pro Thr Gln Leu Arg
690 695 700
Thr Pro Ser Pro Gly Lys Leu Val Lys Phe Leu Val Glu Asn Gly Asp
705 710 715 720
Gln Ile Lys Val Gly Gln Pro Tyr Ala Glu Ile Glu Val Met Lys Met
725 730 735
Gln Met Pro Leu Val Ser Gln Glu Asn Gly Val Ile Gln Leu Leu Lys
740 745 750
Gln Pro Gly Ser Thr Ile Asp Ala Gly Asp Ile Ile Ala Met Leu Ala
755 760 765
Leu Asp Asp Pro Ser Lys Val Lys His Ala Met Pro Phe Glu Gly Met
770 775 780
Leu Pro Glu Leu Gly Ser Pro Met Val Glu Gly Thr Lys Pro Ala Tyr
785 790 795 800
Lys Phe His Ser Leu Val Ser Thr Leu Glu Asn Ile Leu Lys Gly Tyr
805 810 815
Asp Asn Gln Val Ile Met Asn Ala Ser Leu Gln Gln Leu Ile Glu Val
820 825 830
Leu Arg Asp Pro Arg Leu Pro Tyr Ser Glu Trp Lys Leu Asn Val Ser
835 840 845
Ala Leu His Thr Arg Leu Pro Leu Ala Leu Asp Glu Lys Leu Asp Lys
850 855 860
Leu Val Glu Arg Ser Ala Asn Arg Ser Ala Ile Phe Pro Ala Lys Gln
865 870 875 880
Leu Gln Lys Met Ile Asp Asp Ala Val Arg Asp Pro Gln Ala Asp Pro
885 890 895
Leu Leu Ser Ser Ile Val Glu Pro Leu Leu Asp Ile Thr Thr Arg Tyr
900 905 910
Ala Asn Gly Leu Glu Ala His Glu His Ser Val Phe Val Lys Phe Leu
915 920 925
Glu Glu Tyr Tyr Asn Val Glu Lys Leu Phe Ser Glu His Gly Glu Arg
930 935 940
Ala Glu Asn Val Ile Leu Lys Leu Arg Asp Glu Asn Ile Asp Asp Leu
945 950 955 960
Asp Lys Val Val Leu Ile Val Leu Ser His Ala Lys Val Ser Ala Lys
965 970 975
Asn Thr Leu Ile Leu Ala Ile Leu Lys His Tyr Gln Pro Ile Cys Lys
980 985 990
Met Ser Ser Ser Val Ala His Ile Phe Thr Lys Pro Leu Gln His Ile
995 1000 1005
Val Glu Leu Glu Ser Lys Pro Ala Ala Lys Val Ala Leu Gln Ala
1010 1015 1020
Arg Glu Ile Leu Ile Gln Gly Ala Leu Pro Ser Val Lys Glu Arg
1025 1030 1035
Thr Glu Gln Ile Glu His Ile Leu Arg Ser Ser Val Thr Asn Thr
1040 1045 1050
Ser Tyr Gly Asp Ser Ser Pro Lys Val Ser Glu Pro Asn Met Glu
1055 1060 1065
Ile Leu Lys Asp Leu Ile Asp Ser Asn Tyr Val Val Phe Asp Val
1070 1075 1080
Leu Leu Pro Phe Leu Cys His Glu Asp Pro Val Ile Ala Thr Ala
1085 1090 1095
Ala Gly Gln Val Tyr Val Arg Arg Ala Tyr Arg Ala Tyr Thr Val
1100 1105 1110
Gly Glu Val Lys Ser His Ser Ile Thr Asp Ser Ser Met Asn Pro
1115 1120 1125
Leu Ile Glu Trp Lys Phe Gln Leu Pro Ser Ala Ala Phe Ser Ser
1130 1135 1140
Ile Pro Gln Leu Lys Ser Lys Ile Gly Met Asn Arg Ala Met Ser
1145 1150 1155
Val Ser Asp Leu Ser Tyr Val Val Asp Ser Glu His Ala Pro Leu
1160 1165 1170
Arg Thr Gly Ile Leu Val Ala Ala Glu His Leu Asp Glu Val Asp
1175 1180 1185
Ala Ser Leu Ala Gln Gly Leu Glu Val Ile Pro Glu His Ser Ala
1190 1195 1200
Pro Gly Asn Gly Pro Ala Pro Asp Arg Ser Gly Ser Ser Ser Ser
1205 1210 1215
Leu Ser Asn Ile Val Asn Val Cys Val Ala Ser Thr Glu Gly Phe
1220 1225 1230
Asp Ser Glu Lys Glu Ile Leu Ala Arg Val Asn Glu Ile Leu Glu
1235 1240 1245
Leu Asn Lys Ala Asp Leu Ile Lys Ser Ser Ile Arg Arg Ile Thr
1250 1255 1260
Phe Met Phe Gly Phe Pro Asp Gly Thr Tyr Pro Lys Tyr Tyr Thr
1265 1270 1275
Phe Asn Gly Pro Asn Tyr Gln Glu Asn Gln Thr Ile Arg His Ile
1280 1285 1290
Glu Pro Ala Cys Ala Phe Gln Leu Glu Leu Gly Arg Met Ser Asn
1295 1300 1305
Phe Asn Ile Thr Pro Ile Phe Thr Asp Asn Arg Asn Ile His Val
1310 1315 1320
Tyr Glu Ala Val Ser Lys Thr Ser Ala Leu Asp Lys Arg Phe Phe
1325 1330 1335
Thr Arg Gly Ile Ile Arg Thr Gly Arg Ile Arg Asp Glu Ile Ser
1340 1345 1350
Ile Gln Glu Tyr Leu Thr Ser Glu Ala Asn Lys Leu Met Ser Asp
1355 1360 1365
Ile Leu Asp Asn Leu Glu Ile Ile Asp Thr Ser Asn Ser Asp Leu
1370 1375 1380
Asn His Ile Phe Ile Asn Phe Ser Ala Val Phe Asp Val Ser Pro
1385 1390 1395
Glu Asp Val Glu Ala Ala Phe Gly Gly Phe Leu Glu Arg Phe Gly
1400 1405 1410
Lys Arg Phe Leu Arg Leu Arg Val Ser Ala Ala Glu Ile Arg Ile
1415 1420 1425
Ile Ile Gln Asp Pro Lys Thr Gly Ala Pro Val Pro Leu Arg Ala
1430 1435 1440
Leu Ile Asn Asn Val Ser Gly Tyr Val Val Lys Ser Glu Leu Tyr
1445 1450 1455
Thr Glu Ile Lys Asn Ala Lys Gly Glu Trp Val Phe Lys Ser Leu
1460 1465 1470
Gly Lys Pro Gly Ser Met His Leu Arg Pro Ile Ala Thr Pro Tyr
1475 1480 1485
Pro Val Lys Glu Trp Leu Gln Pro Lys Arg Tyr Lys Ala His Leu
1490 1495 1500
Met Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu Leu Phe His Gln
1505 1510 1515
Ala Ala Ile Thr Thr Trp Gln Lys Tyr Lys Thr Asn Ser Ser Gln
1520 1525 1530
Lys Asp Leu Thr Thr Met Lys Glu Arg Asp Asp Leu Phe Ile Ser
1535 1540 1545
His Glu Leu Ile Glu Asp Glu Ser Gly Glu Leu Thr Glu Val Glu
1550 1555 1560
Arg Glu Pro Gly Ala Asn Ser Ile Gly Met Val Ala Phe Lys Val
1565 1570 1575
Thr Met Lys Thr Pro Glu Tyr Pro Arg Gly Arg Gln Phe Val Ile
1580 1585 1590
Val Ala Asn Asp Ile Thr Tyr Lys Ile Gly Ser Phe Gly Pro Gln
1595 1600 1605
Glu Asp Glu Phe Phe Asn Lys Val Thr Glu Tyr Ala Arg Lys Arg
1610 1615 1620
Gly Ile Pro Arg Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg Ile
1625 1630 1635
Gly Ile Ala Glu Glu Leu Val Pro Leu Tyr Gln Val Ala Trp Lys
1640 1645 1650
Asp Ala Asp Val Pro Ala Lys Gly Phe Glu Tyr Ile Tyr Leu Asn
1655 1660 1665
Lys Asp Glu Met Glu Thr Leu Lys Gly His Ser Lys Glu Asn Ser
1670 1675 1680
Val Ile Thr Glu Arg Ile Val Glu Asp Gly Glu Glu Arg Phe Val
1685 1690 1695
Ile Lys Ala Ile Ile Gly Ala Asp Asp Gly Leu Gly Val Glu Cys
1700 1705 1710
Leu Arg Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr
1715 1720 1725
Gln Asp Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly
1730 1735 1740
Ile Gly Ala Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Ile
1745 1750 1755
Glu Gly Gln Pro Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys
1760 1765 1770
Val Leu Gly Arg Glu Val Tyr Ser Ser Asn Leu Gln Leu Gly Gly
1775 1780 1785
Thr Gln Ile Met Tyr Asn Asn Gly Val Ser His Leu Thr Ala Ser
1790 1795 1800
Asp Asp Leu Gln Ala Val Glu Gln Ile Ile Lys Trp Leu Ser Tyr
1805 1810 1815
Val Pro Ala Lys Arg Asn Met Pro Val Pro Ile Leu Glu Asn Gly
1820 1825 1830
Asp Lys Trp Asp Arg Gln Ile Asp Tyr Arg Pro Thr Val Asn Glu
1835 1840 1845
Gln Tyr Asp Val Arg Trp Met Ile Glu Gly Arg Glu Leu Glu Asp
1850 1855 1860
Gly Arg Phe Glu Tyr Gly Met Phe Asp Lys Gly Ser Phe Phe Glu
1865 1870 1875
Thr Leu Ser Gly Trp Ala Lys Gly Val Val Val Gly Arg Ala Arg
1880 1885 1890
Leu Gly Gly Ile Pro Ile Gly Val Ile Gly Val Glu Thr Arg Thr
1895 1900 1905
Ile Glu Asn Val Ile Pro Ala Asp Pro Ala Asn Pro Asp Ser Thr
1910 1915 1920
Glu Ala Val Val Gln Glu Ala Gly Gln Val Trp Tyr Pro Asn Ser
1925 1930 1935
Ala Phe Lys Thr Ala Gln Ala Ile Lys Asp Phe Asn His Gly Glu
1940 1945 1950
Gln Leu Pro Leu Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly
1955 1960 1965
Gly Gln Arg Asp Met Tyr Asn Glu Val Leu Lys Tyr Gly Ser Phe
1970 1975 1980
Ile Val Asp Ser Leu Val Asp Tyr Lys Gln Pro Ile Phe Ile Tyr
1985 1990 1995
Ile Pro Pro Thr Gly Glu Leu Arg Gly Gly Ser Trp Val Val Val
2000 2005 2010
Asp Pro Thr Ile Asn Ala Asp Gln Met Glu Met Tyr Ala Asp Lys
2015 2020 2025
Asp Ser Arg Ala Gly Val Leu Glu Pro Ser Gly Met Val Gly Ile
2030 2035 2040
Lys Tyr Arg Arg Glu Lys Leu Leu Ala Thr Met Thr Arg Leu Asp
2045 2050 2055
Thr Thr Tyr Arg Glu Leu Lys Glu Gln Leu Ser Asp Lys Thr Leu
2060 2065 2070
Thr Pro Ala Gln His Gln Glu Val Ser Val Lys Leu Ala Glu Arg
2075 2080 2085
Glu Lys Phe Leu Leu Pro Ile Tyr Thr Gln Val Ser Val Gln Phe
2090 2095 2100
Ala Asp Leu His Asp Arg Ser Ser Arg Met Val Lys Lys Gly Val
2105 2110 2115
Ile Arg Ala Glu Leu Thr Trp Glu Glu Ser Arg Arg Phe Phe Phe
2120 2125 2130
Trp Arg Leu Arg Arg Lys Leu Asp Glu Glu Tyr Leu Val Lys Arg
2135 2140 2145
Leu Asp Ile Glu Leu Pro His Ala Ser Arg Leu Glu Lys Leu Ala
2150 2155 2160
Arg Leu Arg Ser Trp Tyr Pro Ala Ser Ile Asp Glu Thr Lys Asp
2165 2170 2175
Arg Asp Val Val Thr Trp Ile Glu Glu Asn Tyr Asn Val Phe Glu
2180 2185 2190
Glu Glu Phe Lys Ala Leu Lys Leu Glu Ser Phe Ala Gln Glu Leu
2195 2200 2205
Ala Lys Ser Met Arg Thr Asp His Asp Val Thr Met Arg Gly Leu
2210 2215 2220
Glu Glu Ile Ala Arg Met Leu Ser Lys Glu Asp Lys Glu Arg Leu
2225 2230 2235
Leu Ser Ala Leu Lys
2240
<210> 107
<211> 1229
<212> PRT
<213> HPDH-MCRbs
<400> 107
Met Thr Ala Gly Lys Gly Lys Gly Ala Lys Val Asp Pro Ala Gly Gly
1 5 10 15
Lys Ala Ala Thr Gly Arg Leu Ala Gly Lys Val Ala Leu Val Thr Gly
20 25 30
Ala Ala Gly Asn Leu Gly Gly Ala Ile Ile Arg Arg Phe Ile Ala Glu
35 40 45
Gly Ala Thr Leu Val Met Thr Gly Arg Thr Lys Glu Arg Leu Ala Ala
50 55 60
Ala Lys Gln Ala Leu Leu Asp Glu Thr Gly Val Glu Glu Ala Arg Val
65 70 75 80
Phe Thr Val Ile Leu Asp Gly Ala Asp Pro Asp Ser Val Arg Ala Gly
85 90 95
Ile Asp Gln Val Arg Ala Ala Leu Gly Arg Ile Asp Val Leu Val Asn
100 105 110
Asn Ala Gly Ser Ala Gly Pro Arg Gln Thr Leu Glu Asn Val Pro Leu
115 120 125
Thr Arg Ala Glu Met Glu Ala Asn Gly Asp Ser Glu Thr Val Ala Asp
130 135 140
Ala Met Arg Asn Leu Leu Gly Val Ser Trp Asn Leu Thr Arg Ala Ala
145 150 155 160
Ala Pro Ile Met Pro Val Gly Ala Ser Val Val Asn Ile Ser Thr Ile
165 170 175
Phe Ser His Thr Arg Tyr Tyr Gly Arg Thr Ala Tyr Val Val Pro Lys
180 185 190
Ala Ala Leu Asn Ala Leu Ser Gln Glu Leu Ala Lys Glu Leu Gly Ala
195 200 205
Ser Gly Ile Arg Val Asn Thr Val Phe Pro Gly Pro Ile Glu Ser Glu
210 215 220
Arg Ile Arg Thr Val Phe Ala Ala Met Asp Ala Leu Gln Gly Glu Pro
225 230 235 240
Glu Asn Ser Thr Ala Asn His Phe Met Gly Arg Met Thr Leu Glu Arg
245 250 255
Ala Ile Gly Asp Ala Pro Lys Arg Lys Thr Leu Pro Val Pro Gly Asp
260 265 270
Ile Ala Asp Ala Cys Leu Phe Leu Ala Ser Asp Glu Ser Ala Ala Leu
275 280 285
Asn Gly Asn Glu Ile Asp Val Thr His Gly Met Gln Val Arg Lys Asp
290 295 300
Ser Arg Ser Thr Tyr Met Thr Arg Pro Ser Met Arg Ser Leu Asp Gly
305 310 315 320
Ala Gly Leu Thr Val Leu Ile Ala Ala Gly Glu Ala Trp Glu Asp Ala
325 330 335
Leu Asp Met Ala Lys Ile Gln Ala Ala Cys Gly Ala Arg Val Leu Leu
340 345 350
Gly Met Thr Arg Gln Val Asp Val Ala Gln Ala Glu Ala Arg Val Lys
355 360 365
Ala Gln Gly Ala Glu Glu Gly Leu Thr Ile Met Arg Phe Asn Arg Ala
370 375 380
Glu Pro Asp Gly Met Glu Ala Ala Ile Arg Ala Tyr Ser Asp Ala His
385 390 395 400
Gly Ala Ile His Ser Ala Ile Val Leu Pro Val Lys Pro Ala Gly His
405 410 415
Phe Gly Asp Ser Leu Val Glu Ala Ser Asp Ala Glu Val Asp Ala Leu
420 425 430
Ile Asp Ile Glu Leu Thr Gly Ala Ile Ala Val Ala Arg Thr Leu Arg
435 440 445
Arg Tyr Trp Lys Thr Cys Ser Asp Leu Met Gln Asp Pro Arg Phe Val
450 455 460
Phe Met Ser Gly Pro Ser Asp Gly Lys Gly Asn Lys Ala Gly Arg Ile
465 470 475 480
Leu Ala Ala Ala Ile Ala Gln Leu Ile Thr Ile Trp Arg Asp Glu Ser
485 490 495
Arg Val Asp Phe Ala Gln Gly Arg Gln Lys Arg Ala Ala Trp Gly Asn
500 505 510
Gln Ile Val Arg Tyr Thr Asn Glu Glu Asp Glu Asn Thr Arg Phe Ala
515 520 525
Ala Gly His Ala Thr Arg Ile Val Phe Lys Glu Gln Lys Ile Thr Glu
530 535 540
Thr Ser Leu Tyr Leu Pro Ala Asn Ile Gly Glu Glu Thr Gly Ala Arg
545 550 555 560
Arg Ala Met Leu Gly Phe Ala Glu Asn Ile Thr Gly Leu His Leu Gly
565 570 575
Lys Val Ala Met Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Val
580 585 590
Ala Arg Leu Leu Ala Leu Ala Gly Ala Lys Val Met Met Val Ala Arg
595 600 605
Arg Ala Ser Glu Leu Glu Ala Ala Arg Glu Arg Ile Val Gly Glu Leu
610 615 620
Gln Asp Val Gly Phe Ser Gly Val Glu Arg Arg Val Lys Ile Met Ala
625 630 635 640
Asp Val Asp Val Ser Asp Phe Asp Ser Leu Arg Arg Ala Ala Asp Ala
645 650 655
Thr Met Ala Glu Phe Gly Arg Ile Asp Tyr Leu Ile Asn Asn Ala Gly
660 665 670
Val Ala Gly Ala Glu Asp Met Val Ile Asp Met Ser Leu Asp Met Trp
675 680 685
Arg Trp Thr Leu Asp Ala Asn Leu Ile Ser Asn Tyr Leu Leu Met His
690 695 700
Tyr Val Val Pro Ile Met Lys Arg Gln Gly Ser Gly Tyr Ile Leu Asn
705 710 715 720
Val Ser Ser Tyr Phe Gly Gly Glu Lys Phe Leu Ala Val Ala Tyr Pro
725 730 735
Asn Arg Ala Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Val
740 745 750
Glu Ser Phe Ser Arg Phe Leu Gly Pro Glu Ile Gln Val Asn Ala Ile
755 760 765
Ala Pro Gly Pro Val Asp Gly Asp Arg Leu Ser Gly Thr Gly Gly Lys
770 775 780
Pro Gly Leu Phe Leu Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg
785 790 795 800
Leu Asn Ala Ile Tyr Ala Ala Val Ile Glu Ser Ile Arg Glu Gly Ala
805 810 815
Glu Val Thr His Val Leu Gly Arg Leu Ala Arg Asn Asp Thr Gly Tyr
820 825 830
Leu Ser His Asp Arg Ser Thr Pro Asp Pro Leu Arg Leu Leu Cys Leu
835 840 845
Glu Leu Ala Arg Glu Gly Asp Gly Ile Cys Thr Trp Asp Arg Tyr Leu
850 855 860
Leu Thr Pro Asp Ile Ala Gly Arg Leu Leu Met Arg Leu Ala Ser Ala
865 870 875 880
Gly Tyr Leu Leu Ala His Glu Glu Trp Gln Gln Arg Ser Ala Ser Ala
885 890 895
Glu Gly Arg Thr Gly Trp Leu Leu Ala Leu Pro Pro Asp Asp Met Pro
900 905 910
Tyr Leu Pro Gln Glu Arg Ile Gly Lys Glu Ala Asp Lys Val Gly Arg
915 920 925
Gly Val Leu Ser Gln Leu His Leu Gly Ala Met Pro Lys Glu Ser Glu
930 935 940
Val Ala Gln Ala Thr Val Phe Tyr Leu Ala Asp Arg Ala Val Ser Gly
945 950 955 960
Glu Thr Phe Met Pro Ser Gly Gly Leu Ser Val Glu Arg Ser Tyr Thr
965 970 975
Glu Arg Glu Met Phe Gly Ser Pro Lys Gln Glu Arg Leu Glu Gln Met
980 985 990
Lys Gly Lys Thr Val Trp Val Ile Gly Glu His Leu Ala Asp Tyr Ile
995 1000 1005
Ala Glu Thr Ala Asn His Leu Ile Gly Gly Leu Gly Val Ala Arg
1010 1015 1020
Leu Val Leu Leu Thr Arg Ser Glu Ala Gly Gly Ala Ser Val Leu
1025 1030 1035
Ala Leu Leu Asp Pro Glu Ile Ala Glu Arg Val Thr Val Arg Leu
1040 1045 1050
Cys Gly Ala Asp Val Glu Ser Ala Met Asp Gln Ala Leu Thr Asp
1055 1060 1065
Phe Gly Arg Pro Thr Thr Val Val Ser Thr Pro Met Ala Gly Leu
1070 1075 1080
Pro Asp Arg Leu Phe Glu Asp Gly Ala Met Leu Glu Pro Glu Glu
1085 1090 1095
Phe Arg Thr Leu Cys Glu Glu Asn Leu Thr Gln His Phe Arg Val
1100 1105 1110
Ser Arg Lys Ala Ser Leu Tyr Asp Gly Cys Gln Leu Val Leu Val
1115 1120 1125
Ser Pro Asp Val Pro Phe Gly Gln Ser Gly Pro Ala Phe Ala Leu
1130 1135 1140
Ala Asn Phe Val Lys Thr Thr Leu His Ala Phe Thr Ala Thr Leu
1145 1150 1155
Ala Val Glu Asn Glu Arg Leu Val His Asp Val Pro Thr Asn Gln
1160 1165 1170
Ile Asn Leu Thr Arg Arg Val Arg Ser Glu Glu Pro Arg Asn Ala
1175 1180 1185
Val Glu His Gln Glu Glu Leu Lys Arg Phe Ala Arg Ala Val Leu
1190 1195 1200
Leu Val Gly Ala Pro Leu Pro Asp Ala Gln Asp Ser Arg Tyr Arg
1205 1210 1215
Ala Arg Ile Tyr Arg Gly Thr Ser Met Thr Val
1220 1225
<210> 108
<211> 1220
<212> PRT
<213> HPDH-MCRca
<400> 108
Met Ser Gly Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly
1 5 10 15
Gly Ala Gly Asn Ile Gly Ser Glu Leu Thr Arg Arg Phe Leu Ala Glu
20 25 30
Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Arg Ala Lys Leu Thr Ala
35 40 45
Leu Ala Glu Arg Met Gln Ala Glu Ala Gly Val Pro Ala Lys Arg Ile
50 55 60
Asp Leu Glu Val Met Asp Gly Ser Asp Pro Val Ala Val Arg Ala Gly
65 70 75 80
Ile Glu Ala Ile Val Ala Arg His Gly Gln Ile Asp Ile Leu Val Asn
85 90 95
Asn Ala Gly Ser Ala Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu
100 105 110
Thr Glu Ala Glu Leu Gly Pro Gly Ala Glu Glu Thr Leu His Ala Ser
115 120 125
Ile Ala Asn Leu Leu Gly Met Gly Trp His Leu Met Arg Ile Ala Ala
130 135 140
Pro His Met Pro Val Gly Ser Ala Val Ile Asn Val Ser Thr Ile Phe
145 150 155 160
Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Thr Pro Lys Ala
165 170 175
Ala Leu Asn Ala Leu Ser Gln Leu Ala Ala Arg Glu Leu Gly Ala Arg
180 185 190
Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Asp Arg
195 200 205
Ile Arg Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu
210 215 220
Gly Asp Thr Ala His His Phe Leu Asn Thr Met Arg Leu Cys Arg Ala
225 230 235 240
Asn Asp Gln Gly Ala Leu Glu Arg Arg Phe Pro Ser Val Gly Asp Val
245 250 255
Ala Asp Ala Ala Val Phe Leu Ala Ser Ala Glu Ser Ala Ala Leu Ser
260 265 270
Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Ala Cys Ser
275 280 285
Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Ser
290 295 300
Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met
305 310 315 320
Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly
325 330 335
Phe Arg Ser Ala Ala Ala Leu Ala Gln Phe Glu Gln Ala Val Asn Glu
340 345 350
Ser Arg Arg Leu Ala Gly Ala Asp Phe Thr Pro Pro Ile Ala Leu Pro
355 360 365
Leu Asp Pro Arg Asp Pro Ala Thr Ile Asp Ala Val Phe Asp Trp Gly
370 375 380
Ala Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala
385 390 395 400
Thr Ser His Glu Pro Ala Pro Cys Val Ile Glu Val Asp Asp Glu Arg
405 410 415
Val Leu Asn Phe Leu Ala Asp Glu Ile Thr Gly Thr Ile Val Ile Ala
420 425 430
Ser Arg Leu Ala Arg Tyr Trp Gln Ser Gln Arg Leu Thr Pro Gly Ala
435 440 445
Arg Ala Arg Gly Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Asp Gln
450 455 460
Asn Gly Asn Val Tyr Gly Arg Ile Gln Ser Ala Ala Ile Gly Gln Leu
465 470 475 480
Ile Arg Val Trp Arg His Glu Ala Glu Leu Asp Tyr Gln Arg Ala Ser
485 490 495
Ala Ala Gly Asp His Val Leu Pro Pro Val Trp Ala Asn Gln Ile Val
500 505 510
Arg Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp
515 520 525
Thr Ala Gln Leu Leu His Ser Gln Arg His Ile Asn Glu Ile Thr Leu
530 535 540
Asn Ile Pro Ala Asn Ile Ser Ala Thr Thr Gly Ala Arg Ser Ala Ser
545 550 555 560
Val Gly Trp Ala Glu Ser Leu Ile Gly Leu His Leu Gly Lys Val Ala
565 570 575
Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu
580 585 590
Leu Ala Leu Ser Gly Ala Arg Val Met Leu Ala Ala Arg Asp Arg His
595 600 605
Lys Leu Glu Gln Met Gln Ala Met Ile Gln Ser Glu Leu Ala Glu Val
610 615 620
Gly Tyr Thr Asp Val Glu Asp Arg Val His Ile Ala Pro Gly Cys Asp
625 630 635 640
Val Ser Ser Glu Ala Gln Leu Ala Asp Leu Val Glu Arg Thr Leu Ser
645 650 655
Ala Phe Gly Thr Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly
660 665 670
Val Glu Glu Met Val Ile Asp Met Pro Val Glu Gly Trp Arg His Thr
675 680 685
Leu Phe Ala Asn Leu Ile Ser Asn Tyr Ser Leu Met Arg Lys Leu Ala
690 695 700
Pro Leu Met Lys Lys Gln Gly Ser Gly Tyr Ile Leu Asn Val Ser Ser
705 710 715 720
Tyr Phe Gly Gly Glu Lys Asp Ala Ala Ile Pro Tyr Pro Asn Arg Ala
725 730 735
Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Ala Glu Val Phe
740 745 750
Ala Arg Phe Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly
755 760 765
Pro Val Glu Gly Asp Arg Leu Arg Gly Thr Gly Glu Arg Pro Gly Leu
770 775 780
Phe Ala Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu
785 790 795 800
Leu His Ala Ala Leu Ile Ala Ala Ala Arg Thr Asp Glu Arg Ser Met
805 810 815
His Glu Leu Val Glu Leu Leu Leu Pro Asn Asp Val Ala Ala Leu Glu
820 825 830
Gln Asn Pro Ala Ala Pro Thr Ala Leu Arg Glu Leu Ala Arg Arg Phe
835 840 845
Arg Ser Glu Gly Asp Pro Ala Ala Ser Ser Ser Ser Ala Leu Leu Asn
850 855 860
Arg Ser Ile Ala Ala Lys Leu Leu Ala Arg Leu His Asn Gly Gly Tyr
865 870 875 880
Val Leu Pro Ala Asp Ile Phe Ala Asn Leu Pro Asn Pro Pro Asp Pro
885 890 895
Phe Phe Thr Arg Ala Gln Ile Asp Arg Glu Ala Arg Lys Val Arg Asp
900 905 910
Gly Ile Met Gly Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe Asp
915 920 925
Val Ala Met Ala Thr Val Tyr Tyr Leu Ala Asp Arg Asn Val Ser Gly
930 935 940
Glu Thr Phe His Pro Ser Gly Gly Leu Arg Tyr Glu Arg Thr Pro Thr
945 950 955 960
Gly Gly Glu Leu Phe Gly Leu Pro Ser Pro Glu Arg Leu Ala Glu Leu
965 970 975
Val Gly Ser Thr Val Tyr Leu Ile Gly Glu His Leu Thr Glu His Leu
980 985 990
Asn Leu Leu Ala Arg Ala Tyr Leu Glu Arg Tyr Gly Ala Arg Gln Val
995 1000 1005
Val Met Ile Val Glu Thr Glu Thr Gly Ala Glu Thr Met Arg Arg
1010 1015 1020
Leu Leu His Asp His Val Glu Ala Gly Arg Leu Met Thr Ile Val
1025 1030 1035
Ala Gly Asp Gln Ile Glu Ala Ala Ile Asp Gln Ala Ile Thr Arg
1040 1045 1050
Tyr Gly Arg Pro Gly Pro Val Val Cys Thr Pro Phe Arg Pro Leu
1055 1060 1065
Pro Thr Val Pro Leu Val Gly Arg Lys Asp Ser Asp Trp Ser Thr
1070 1075 1080
Val Leu Ser Glu Ala Glu Phe Ala Glu Leu Cys Glu His Gln Leu
1085 1090 1095
Thr His His Phe Arg Val Ala Arg Lys Ile Ala Leu Ser Asp Gly
1100 1105 1110
Ala Ser Leu Ala Leu Val Thr Pro Glu Thr Thr Ala Thr Ser Thr
1115 1120 1125
Thr Glu Gln Phe Ala Leu Ala Asn Phe Ile Lys Thr Thr Leu His
1130 1135 1140
Ala Phe Thr Ala Thr Ile Gly Val Glu Ser Glu Arg Thr Ala Gln
1145 1150 1155
Arg Ile Leu Ile Asn Gln Val Asp Leu Thr Arg Arg Ala Arg Ala
1160 1165 1170
Glu Glu Pro Arg Asp Pro His Glu Arg Gln Gln Glu Leu Glu Arg
1175 1180 1185
Phe Ile Glu Ala Val Leu Leu Val Thr Ala Pro Leu Pro Pro Glu
1190 1195 1200
Ala Asp Thr Arg Tyr Ala Gly Arg Ile His Arg Gly Arg Ala Ile
1205 1210 1215
Thr Val
1220
<210> 109
<211> 1219
<212> PRT
<213> HPDH-MCRcag
<400> 109
Met Ser Val Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly
1 5 10 15
Gly Ala Gly Asn Ile Gly Ser Glu Met Thr Arg Arg Phe Leu Ala Glu
20 25 30
Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Ser Ala Lys Leu Ala Ala
35 40 45
Leu Ala Glu Arg Leu Arg Ser Glu Ala Gly Val Pro Ala Lys Arg Ile
50 55 60
Asp Leu Glu Val Met Asp Gly Ser Asp Pro Ala Ala Val Arg Ala Gly
65 70 75 80
Val Ala Ala Ile Ile Gly Arg His Gly His Ile Asp Ile Leu Val Asn
85 90 95
Asn Ala Gly Ser Thr Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu
100 105 110
Asn Glu Thr Asp Arg Asp Leu Asp Asp Glu Glu Ala Leu Ser Thr Ser
115 120 125
Val Ala Asn Leu Leu Gly Met Ala Trp His Leu Met Arg Ile Leu Ser
130 135 140
Pro His Met Pro Pro Gly Ser Ala Ile Ile Asn Ile Ser Thr Ile Phe
145 150 155 160
Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ala
165 170 175
Ala Leu Asn Thr Leu Thr Gln Ile Ala Ala Arg Glu Leu Gly Ile Arg
180 185 190
Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Glu Arg
195 200 205
Ile Gln Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu
210 215 220
Gly Asp Thr Ala Ser Gln Phe Leu Ala Thr Met Arg Leu Tyr Arg Ala
225 230 235 240
Asn Asp Gln Gly Gln Leu Glu Arg Arg Phe Pro Thr Ile Cys Asp Val
245 250 255
Ala Asp Ala Ala Val Phe Leu Ala Ser Asp Glu Ala Ala Ala Leu Thr
260 265 270
Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Thr Ser Ser
275 280 285
Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Asn
290 295 300
Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met
305 310 315 320
Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly
325 330 335
Phe Arg Ser Glu Ala Ala Leu Ala Gln Phe Glu Gln Ala Ile Gly Glu
340 345 350
Ser Arg Arg Leu Ala Gly Glu Ser Phe Ile Pro Pro Ile Ala Leu Pro
355 360 365
Ile Asp Leu Arg Asn Pro Ser Thr Ile Asp Ala Leu Phe Asp Trp Ala
370 375 380
Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala Ser
385 390 395 400
Gly Arg Glu Pro Ala Thr Gln Val Ile Asp Ile Asp Asp Ala His Val
405 410 415
Gln Ala Phe Leu Asn Asp Glu Ile Val Gly Ser Ile Ile Ile Ala Ser
420 425 430
Arg Leu Ala Arg Tyr Trp Gln Ala Gln Arg Ile Ala Pro Gly Ala Arg
435 440 445
Ala Arg Glu Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Ser Thr Ala
450 455 460
Gly Asn Pro Tyr Gly Arg Ile Gln Ser Ala Ala Ile Glu Gln Leu Ile
465 470 475 480
Arg Val Trp Arg His Glu Ala Ala Leu Asp Tyr Glu Arg Ala Thr Ala
485 490 495
Ala Gly Glu Arg Val Leu Pro Ala Val Trp Ala Ser Gln Ile Val Arg
500 505 510
Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp Thr
515 520 525
Ala Gln Leu Leu His Ser Gln Arg Arg Ile Asn Glu Ile Thr Leu Thr
530 535 540
Ile Pro Ala Asp Ile Ser Ala Thr Thr Gly Ala Arg Ser Ala Ser Val
545 550 555 560
Gly Trp Ala Glu Ser Leu Ile Gly Leu His Leu Gly Lys Val Ala Leu
565 570 575
Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu Leu
580 585 590
Ala Leu Ser Gly Ala Arg Val Met Leu Ala Ala Arg Asp Pro His Lys
595 600 605
Leu Glu Gln Ile Gln Ala Thr Ile Arg Ala Glu Leu Ala Glu Val Gly
610 615 620
Tyr Thr Asp Val Glu Glu Arg Val Gln Ile Ala Pro Gly Cys Asp Val
625 630 635 640
Ser Ser Glu Glu Gln Leu Val Asp Leu Val Glu Arg Thr Leu Ala Ala
645 650 655
Phe Gly Thr Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly Val
660 665 670
Glu Glu Met Val Ile Asp Met Pro Val Glu Gly Trp Arg Asn Thr Leu
675 680 685
Tyr Ala Asn Leu Ile Ser Asn Tyr Ser Leu Met Arg Lys Leu Ala Pro
690 695 700
Leu Met Lys Lys Gln Gly Ser Gly Tyr Val Leu Asn Val Ser Ser Tyr
705 710 715 720
Phe Gly Gly Glu Lys Asp Ala Ala Ile Pro Tyr Pro Asn Arg Ala Asp
725 730 735
Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Ala Glu Val Phe Ala
740 745 750
Arg Phe Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly Pro
755 760 765
Val Glu Gly Asp Arg Leu Arg Gly Thr Gly Glu Arg Pro Gly Leu Phe
770 775 780
Ala Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu Leu
785 790 795 800
His Ala Ala Leu Ile Thr Ala Ala Arg Thr Asp Asn Arg Pro Met Arg
805 810 815
Glu Leu Val Glu Leu Leu Leu Pro Asn Asp Val Ala Ala Leu Ala Gln
820 825 830
His Pro Ala Ala Pro Asp Val Leu Arg Thr Leu Ala Lys Arg Phe Gln
835 840 845
Ser Glu Gly Asp Pro Ala Ala Ser Ser Ser Ser Phe Leu Leu Asn Arg
850 855 860
Ser Ile Ala Ala Lys Leu Leu Ala Arg Leu Ile Asn Gly Gly Tyr Asp
865 870 875 880
Leu Pro Ala Asp Ile Phe Ala Asn Leu Ala Val Pro Pro Asp Pro Phe
885 890 895
Phe Thr Arg Ala Gln Ile Asp Arg Glu Ala Arg Lys Val Arg Asp Gly
900 905 910
Ile Met Gly Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe Asp Val
915 920 925
Ala Met Ala Thr Val Tyr Tyr Leu Ala Asp Arg Asn Val Ser Gly Glu
930 935 940
Thr Phe His Pro Ser Gly Gly Leu Arg Tyr Glu Arg Thr Pro Thr Gly
945 950 955 960
Gly Glu Leu Phe Gly Leu Pro Ala Pro Glu Arg Leu Ala Glu Leu Val
965 970 975
Gly Ser Thr Val Tyr Leu Ile Gly Glu His Leu Thr Glu His Leu Asn
980 985 990
Leu Leu Ala Arg Ala Tyr Leu Glu Arg Tyr Gly Ala Arg Gln Val Val
995 1000 1005
Met Ile Val Glu Thr Glu Ala Gly Ala Glu Lys Met Arg His Leu
1010 1015 1020
Leu His Asp His Val Glu Ala Gly Arg Leu Pro Ile Ile Val Ala
1025 1030 1035
Gly Asp Gln Ile Glu Ala Ala Ile Asp Gln Ala Ile Ala Asn Tyr
1040 1045 1050
Gly Arg Pro Gly Pro Val Val Cys Thr Pro Phe Arg Pro Leu Pro
1055 1060 1065
Ser Ala Pro Leu Val Gly Arg Lys Asp Ser Asp Trp Ser Thr Val
1070 1075 1080
Leu Ser Glu Ala Glu Phe Ala Glu Leu Cys Glu His Gln Leu Thr
1085 1090 1095
His His Phe Arg Val Ala Arg Lys Ile Ala Leu Ser Asp Gly Ala
1100 1105 1110
Ser Leu Ala Leu Val Thr Pro Glu Thr Thr Ala Thr Ser Ser Thr
1115 1120 1125
Glu Gln Phe Ala Leu Ala Asn Phe Val Lys Thr Thr Leu His Ala
1130 1135 1140
Phe Thr Ala Thr Ile Gly Val Glu Ser Glu Arg Thr Ala Gln Arg
1145 1150 1155
Ile Leu Ile Asn Gln Val Asp Leu Thr Arg Arg Ala Arg Ala Glu
1160 1165 1170
Glu Pro Arg Asp Pro Arg Glu Arg Gln Gln Glu Leu Glu Arg Phe
1175 1180 1185
Ile Glu Ala Val Leu Leu Val Thr Ala Pro Leu Pro Pro Glu Ala
1190 1195 1200
Asp Thr Arg Tyr Ala Gly Arg Ile His Arg Gly Arg Ala Ile Thr
1205 1210 1215
Val
<210> 110
<211> 1231
<212> PRT
<213> HPDH-MCRct
<400> 110
Met Arg Arg Thr Lys Pro Glu Cys Leu Arg Arg Asn Glu Ser Thr Met
1 5 10 15
Arg Leu Asn Gly Lys Val Ala Leu Ile Thr Gly Ala Ala Gly Gly Ile
20 25 30
Gly Ser Val Val Thr Arg Met Tyr Leu Arg Glu Gly Ala Arg Val Ala
35 40 45
Ile Ser Ser Arg Ser Leu Glu Arg Ala Glu Gln Phe Arg Ala Ala Leu
50 55 60
Met Ala Glu Gly Phe Ser Gly Asp Asp Ile Leu Pro Val Ala Phe Ala
65 70 75 80
Ser Thr Asp Leu Ala Gly Met Gln Thr Ala Ile Glu Ala Ile Ala Ala
85 90 95
Arg Trp Gly Gly Leu Asp Val Leu Ile Asn Asn Ala Gly Ser Ala Gly
100 105 110
Ala Lys Gln Pro Leu Phe Arg Ile Pro Phe Thr Ser Glu Asp Leu Ala
115 120 125
Gln Leu Ala Ala Glu Gly Phe Thr Glu Thr Glu Thr Met Arg Glu Ser
130 135 140
Ala Ala Asn Leu Leu Gly Met Pro Trp His Leu Thr Arg Leu Ala Leu
145 150 155 160
Pro Tyr Leu Arg Val Gly Ala Ser Ile Ile Asn Val Ser Thr Ile Phe
165 170 175
Ser Arg Thr Asn Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ser
180 185 190
Ala Leu Asn Ala Leu Ser Leu Gly Leu Ala Lys Gln Leu Gly Thr Thr
195 200 205
Asp Arg Ala Val Arg Val Asn Thr Val Phe Pro Gly Pro Ile Asp Ser
210 215 220
Asp Arg Ile Arg Thr Val Phe Gly Ala Met Asp Arg Leu Lys Gly Val
225 230 235 240
Pro Glu Gly Thr Thr Ala Gln Glu Phe Phe Asp Ile Met Ile Leu Ala
245 250 255
Arg Ala Arg Gly Gly Glu Ala Pro Thr Lys Cys Tyr Pro Thr Gln Ala
260 265 270
Asp Val Ala His Val Ile Leu Phe Leu Gly Ser Asp Glu Ser Ala Ala
275 280 285
Ile Ser Gly His Asn Phe Glu Ile Thr His Gly Met Gln Val Lys Ala
290 295 300
Gln Ser Arg Thr Lys Leu Thr Ala Trp Pro Asp Gln Arg Leu Ile Asp
305 310 315 320
Leu Ser Arg Arg Val Val Leu Ile Val Gly Gly Glu Gln Val His Asp
325 330 335
Ala Val Thr Ile Ala Ala Ser Gln Gln Ser Tyr Gly Ala Ser Val Ile
340 345 350
Leu Thr Phe Arg Gln Pro Glu Ala Val Asn Thr Ala Lys Ala Gln Leu
355 360 365
Glu Ser Arg Gly Leu Asn His Ser Ile Ala Val Thr Trp Leu Asp Pro
370 375 380
Leu Arg Arg Glu Ser Val Thr Ser Val Phe Asp Leu Ile Arg Glu Arg
385 390 395 400
Tyr His Arg Leu Asp Thr Val Ile Val Phe Pro Ala His Ala Ala Gly
405 410 415
His Tyr Gly Pro Glu Leu Ile Pro Asp Glu Pro Glu Arg Ile Arg Gln
420 425 430
Phe Thr Asp Glu Ala Ile Leu Gly Thr Thr Ala Phe Ala Ala Gln Leu
435 440 445
Ser Arg Phe Leu Leu Ala Phe Asp Ala Glu Asn Glu Leu Gln Glu Pro
450 455 460
Val Asn Val Leu Phe Val Thr Asn Glu His Asp Gly Ala Thr Asn Ala
465 470 475 480
Phe Glu Asp Ile Tyr Arg Ala Ala Val Glu Gln Leu Leu Arg Val Trp
485 490 495
Arg His Glu Asp Glu Leu Gln Val Ala Ala Gly Thr Arg Arg Phe Ala
500 505 510
Val Arg Thr Asn Gln Ile Val Arg Tyr Ala Asn Ala Glu Pro Asp Asn
515 520 525
Leu Lys Phe Thr Ala Asp Trp Leu Ala Thr Leu Ala Asn Arg Val Arg
530 535 540
Ile Phe Asp Glu Leu Asn Leu Tyr Val Pro Glu Asn Ile Gln Arg Thr
545 550 555 560
Thr Gly Lys Thr Glu Leu Pro Arg Asp Ile Ala Arg Thr Leu Leu Gly
565 570 575
Leu His Ile Gly Lys Val Ala Val Ile Thr Gly Gly Ser Ala Gly Ile
580 585 590
Gly Gly Ala Ile Gly His Tyr Leu Ala Leu Ser Gly Ala Lys Val Val
595 600 605
Leu Ala Ala Arg Asp Ala Glu Arg Leu Ala Ala Leu Lys Arg Glu Ile
610 615 620
Val Ala Glu Leu Phe Ala Val Gly Tyr Pro Gln Pro Glu Thr Arg Val
625 630 635 640
Glu Thr Leu Ala Asn Ile Asp Val Ala Asp Glu Glu Ala Leu Gly Arg
645 650 655
Leu Val Lys Phe Ala Leu Asp Lys Phe Gly Arg Ile Asp Phe Leu Ile
660 665 670
Asn Asn Ala Gly Ile Ala Gly Ala Glu Glu Met Val Val Asp Leu Pro
675 680 685
Leu Ala Ala Trp Arg His Thr Leu Lys Ala Asn Leu Leu Ser Asn Tyr
690 695 700
Ser Leu Ile Tyr Lys Ile Ala Pro Leu Met Lys Lys Gln Gly Ala Gly
705 710 715 720
Tyr Ile Leu Asn Val Ser Ser Tyr Phe Gly Gly Glu Lys Tyr Val Ala
725 730 735
Val Ala Tyr Pro Asn Arg Ser Asp Tyr Ala Val Ser Lys Ala Gly Gln
740 745 750
Arg Ala Leu Ala Glu Ile Leu Ala Arg His Leu Gly Pro Glu Ile Gln
755 760 765
Ile Asn Ala Leu Ser Pro Gly Pro Val Glu Gly Lys Arg Leu Arg Gly
770 775 780
Glu Gly Ser Arg Pro Gly Leu Tyr Ala Arg Arg Ala Arg Leu Ile Leu
785 790 795 800
Glu Asn Lys Arg Leu Asn Asp Ile His Asp Ala Val Ile Arg Ala Trp
805 810 815
Arg Glu Gly Glu Ala Val Lys Ser Thr Leu Gly Ala Leu Ala His Asn
820 825 830
His Phe Glu Asp Val Leu Ala Ala Gly Pro Pro Glu Pro Val Arg Arg
835 840 845
Leu Ile Thr His Leu Arg Ala Ala Ala Val Asn Glu Gly His Ser Asp
850 855 860
Ser His Tyr Leu Met Thr Arg Lys Ile Ala Glu Lys Leu Leu His Arg
865 870 875 880
Leu Phe Glu Gly Gly Tyr Leu Thr Ser Val Glu Asp Arg Glu Ala Phe
885 890 895
Thr Leu Ala Phe Leu Arg Glu Leu Pro Glu Pro Pro Asp Pro Phe Phe
900 905 910
Asp Pro Lys Glu Ile Asp Arg Glu Ala Glu Arg Val Gln Ser Gly Ile
915 920 925
Leu Thr Met Leu Asn Leu Arg Arg Met Pro Thr Glu Glu Glu Ile Ala
930 935 940
Ile Ala Thr Val Phe Tyr Leu Ser Asp Pro Asn Ile Ser Gly Glu Thr
945 950 955 960
Leu His Pro Ser Gly Gly Leu Lys Phe Asp Arg Thr Val Thr Glu Gly
965 970 975
Glu Phe Tyr Gly Leu Pro Arg Asn Glu His Leu Met Glu Leu Arg Arg
980 985 990
Lys Asn Val Leu Leu Ile Gly Asp Tyr Leu Glu Glu Glu Leu Arg Trp
995 1000 1005
Leu Val Gly Ala Phe Ala Ile Gln Leu Val Val Arg Asn Cys Ile
1010 1015 1020
Ile Leu Thr Lys Thr Ala Glu Arg Ala Asp Ser Leu Arg His Tyr
1025 1030 1035
Phe Ser Ala Gln Arg Leu Asn Asn Leu His Ile Phe Ser Ala Gly
1040 1045 1050
Ala Asn Val Glu Ala Ala Met Asp Asp Leu Ile Arg Arg Phe Gly
1055 1060 1065
His Leu Asp Val Val Val Ser Thr Pro Phe Asp Pro Leu Pro Leu
1070 1075 1080
Lys Pro Leu Ala Ala Thr Asp Asp Trp Arg Gly Val Leu Asn Glu
1085 1090 1095
Lys Asp Phe Glu Gln Leu Val Glu Thr His Ile Thr His His Phe
1100 1105 1110
Arg Val Ala Lys Lys Ala Ala Leu Gln Asp Lys Ser Gln Ile Val
1115 1120 1125
Leu Val Thr Pro Lys Thr Ser Arg Asn Ser Ser Arg Glu Glu Phe
1130 1135 1140
Ala Leu Ala Leu Phe Val Lys Thr Thr Leu His Ala Leu Thr Ala
1145 1150 1155
Thr Leu Ala Val Glu Cys Glu Arg Phe Thr His His Pro Thr Val
1160 1165 1170
Asn Gln Val Asp Leu Thr Arg Arg Ala Arg Val Glu Glu Pro Arg
1175 1180 1185
Asn Glu Arg Glu Glu Arg Glu Glu Leu Glu Arg Phe Val Asp Ala
1190 1195 1200
Val Leu Leu Ala Ala Ala Pro Ala Pro Glu Pro Glu Gln Ser Arg
1205 1210 1215
Tyr Leu Ser Arg Ile His Arg Gly Asn Ala Ile Thr Val
1220 1225 1230
<210> 111
<211> 1216
<212> PRT
<213> HPDH-MCRgb
<400> 111
Met Asn Asp Met Ser Asp Ile Pro Gln Asp Leu Leu Thr Gln Ser Ile
1 5 10 15
Ala Gly Arg Arg Leu Asp Asn Lys Cys Ile Ile Leu Thr Gly Ala Ala
20 25 30
Gly Ser Ile Gly Ser Phe Ile Thr Arg Gln Leu Leu Arg Glu Gly Ala
35 40 45
Arg Val Met Leu Thr Gly Arg Asp Gln Ser Lys Leu Asp Asp Phe Ile
50 55 60
Gly Asp Leu Val Ser Glu Gly Phe Glu Glu Thr Phe Met Val Ser Ala
65 70 75 80
Thr Gly Asp Cys Ala Asp Pro Glu Val Cys Arg Glu Ile Val Ala Lys
85 90 95
Thr Val Ala Ala Phe Gly Pro Ile Asp Val Leu Val Asn Asn Ala Gly
100 105 110
Ala Ala Gly Pro Lys Phe Thr Leu Arg Asp Ile Pro Phe Thr Glu Ala
115 120 125
Glu Gln Arg Ala Ala Asp Ser Asp Gln Thr Met Phe Asp Ser Ala Met
130 135 140
Asn Leu Leu Gly Ala Pro Trp Asn Met Ala Arg Ala Ala Ala Pro His
145 150 155 160
Met Ser Ala Gly Ala Ser Ile Ile Asn Val Ser Thr Ile Phe Ser Arg
165 170 175
Thr His Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ser Gly Leu
180 185 190
Asn Ala Leu Gly Lys Gly Leu Ala Leu Glu Leu Gly Glu Lys Gln Gly
195 200 205
Ile Arg Val Asn Thr Leu Phe Pro Gly Pro Ile Glu Ser Glu Arg Ile
210 215 220
Glu Ser Val Phe Ala Arg Met Asp Glu Leu Gln Gly Val Glu Pro Gly
225 230 235 240
Ser Thr Gly Lys Glu Phe Arg Asp Leu Met Ile Thr Thr Arg Thr Ser
245 250 255
Asp Gly Gly Leu Glu Tyr Lys Tyr Pro Thr Pro Thr Asp Val Ala Ser
260 265 270
Gly Ile Val Trp Leu Ala Ser Asp Glu Ser Ser Ala Leu Ser Gly His
275 280 285
His Ile Glu Val Thr Asn Gly Met Gln Val Pro Ala Gln Ser Arg Ser
290 295 300
Gln Leu Val Ser Trp Pro Asp Lys Arg Leu Glu Asp Leu Ser Gly Gln
305 310 315 320
Val Val Leu Ile Leu Gly Gly Asp Asp Tyr Gln Glu Ala Val Thr Phe
325 330 335
Ala Glu Arg Gln Ile Gln Ser Gly Ala Arg Val Met Leu Ala Phe Arg
340 345 350
Asn Leu Asn Thr Val Gly His Ala Arg Ser Leu Val Gln Ser Arg Asn
355 360 365
Leu Gly Asp Val Gln Leu Ser His Leu Asp Pro Leu Arg Arg Glu Ser
370 375 380
Val Asp Arg Ser Met Gln Leu Ile Ala Asp His Phe Gly Arg Leu Asp
385 390 395 400
Gly Val Ile Leu Leu Pro Gln Lys Pro Asn Gly Glu Tyr Gly Tyr Ser
405 410 415
Leu Thr Thr Ala Asn Asp Glu Asp Val Ala Asn Phe Val Arg Asp Glu
420 425 430
Val Val Ala Pro Val Ala Phe Ala Ser Ala Phe Ala Arg Asn Met Asp
435 440 445
Gln Leu Phe Ala Glu Gly Glu Pro Pro Ala Ile Val Tyr Val Thr Asn
450 455 460
Pro Ser Asp Arg His Gly Asn Leu Met Asn Glu Ile Ile Arg Ala Ser
465 470 475 480
Val Glu Ala Leu Ile Arg Gly Trp Arg His Glu Asp Glu Thr Leu Ala
485 490 495
Asn Thr Gly Glu Leu Pro Trp Ala Val Gln Pro Asn Gln Met Val Arg
500 505 510
Tyr Asp Thr Glu Asp Arg Glu Ala Leu Thr Phe Ala Ala Asp Trp Ala
515 520 525
Ala Thr Leu Thr Asn Arg Val Arg Lys Met Asp Ser Ile Asn Leu Trp
530 535 540
Leu Pro Arg Ser Ile Lys Arg Ser Thr Gly Lys Ser Ser Met Pro Ser
545 550 555 560
Ser Ile Ser Arg Val Leu Pro Gly Leu His Lys Gly Arg Thr Ala Val
565 570 575
Ile Thr Gly Gly Ser Leu Gly Ile Gly Leu Gln Leu Gly Arg Phe Leu
580 585 590
Ala Ile Ala Gly Ala Arg Val Leu Leu Ser Ala Arg Ser Glu Gly Lys
595 600 605
Leu Ala Glu Ala Arg Asp Glu Ile Val Asp Glu Leu Arg Asn Ile Gly
610 615 620
Tyr Pro Arg Pro Glu Ser Arg Val Thr Ile Leu Gly Gly Ile Asp Val
625 630 635 640
Gly Asp Pro Asp Ala Leu Asp Arg Leu His Asp His Ala Val Ala Glu
645 650 655
Leu Gly His Val Asp Phe Leu Ile Asn Asn Ala Gly Ile Ser Gly Ala
660 665 670
Glu Glu Met Val Val Asp Met Thr Arg Ala Asp Trp Asp Arg Thr Met
675 680 685
Glu Ala Asn Leu Ile Ser Asn Tyr Ser Leu Ile Arg Lys Phe Ser Pro
690 695 700
Ala Met Lys Ala Gly Gly Lys Gly Ser Ile Leu Asn Val Ser Ser Tyr
705 710 715 720
Phe Gly Gly Glu Lys Tyr Val Ala Val Ala Tyr Pro Asn Arg Ala Asp
725 730 735
Tyr Ala Val Ser Lys Ala Gly Gln Arg Val Leu Ala Glu Ile Leu Ser
740 745 750
Arg His Leu Gly Pro Glu Ile Gln Ile Asn Ala Leu Ala Pro Gly Pro
755 760 765
Val Asp Gly Ala Arg Leu Arg Gly Ser Ala Glu Ala Pro Gly Leu Phe
770 775 780
Asp Arg Arg Gly Arg Leu Val Leu Glu Asn Lys Arg Leu Asn Glu Val
785 790 795 800
His Lys Ala Ile Leu Ala Gly Met Ser Ser Gly Phe Asp Ala Asp Gly
805 810 815
Val Met Ser Leu Ala Ser Asn Ala Val Asp Ser Ile Asp Ile Asp Ser
820 825 830
Leu Pro Lys Pro Val Ala Arg Leu Val Leu Lys Ile Arg Asp Ser Gly
835 840 845
Gly Met Gly Asn Ser Ser His Tyr Leu Met His Met Gly Ile Ala Ala
850 855 860
Lys Leu Met Thr Arg Leu Val Arg Ala Gly Ile Leu Ser Asp Glu Gln
865 870 875 880
Arg Glu Gln Phe Leu Gly Thr Phe Ile Asp Ala Pro Ala Pro Phe Phe
885 890 895
Asp Phe Ser Glu Thr Ser Lys Gln Ala Glu Gln Ile Glu Thr Gly Ile
900 905 910
Leu Asn Arg Leu His Leu His Lys Met Pro Thr Asp Glu Gln Val Gly
915 920 925
Leu Ser Thr Val Phe His Leu Ala Asp Asp Ile Val Ser Gly Glu Thr
930 935 940
Phe His Pro Ser Gly Gly Leu Lys Phe Asp Arg Ser Val Thr Glu Gly
945 950 955 960
Glu Leu Leu Leu Pro Pro Asn Glu Thr Glu Val Ala Lys Leu Lys Gly
965 970 975
Lys Arg Ile Val Leu Ile Gly Asp Ser Met Arg Asn Glu Leu Thr His
980 985 990
Ile Ala Asn Gly Phe Leu Ala Gln Asn Val Glu Lys Leu Trp Val Leu
995 1000 1005
Thr Lys Thr Glu Asp Ser Ala Asn Ala Met Thr His Ser Val Asp
1010 1015 1020
Asn Pro His Gly Ile Asp Ile Glu Cys Arg Ala Ile Gly Asp Asp
1025 1030 1035
Leu Glu Ser Ser Leu Asp Asp Ile Leu Arg Asn Asp Gly Gly Tyr
1040 1045 1050
Asp Val Val Val Ser Ser Pro Phe Glu Arg Leu Pro Leu Asn Ala
1055 1060 1065
Leu Ala Ala Asn Ala Asn Glu Pro Trp Asp Arg Val Leu Ser Asp
1070 1075 1080
His Glu Phe Arg Lys Leu Val His Asp Gln Leu Thr His His Phe
1085 1090 1095
Arg Val Ala Arg Thr Ser Ala Leu Val Pro Asn Cys Gln Ile Val
1100 1105 1110
Leu Ile Thr Pro Asp Thr Ser Leu Ala Ser Thr Arg Glu Glu Phe
1115 1120 1125
Ala Leu Ala Leu Phe Val Lys Asn Ser Leu His Ala Phe Thr Val
1130 1135 1140
Thr Leu Gly Val Glu Gly Glu Arg Leu Pro Thr Val Pro Ala Ile
1145 1150 1155
Asn Gln Val Gln Leu Thr Arg Arg Ala His Thr Glu Glu Pro Ser
1160 1165 1170
Asn Asp Gln Glu Leu Glu Glu Glu Met Thr Arg Leu Val His Ala
1175 1180 1185
Val Leu Gln Cys Ser Val Pro Ala Pro Ala Pro Ser Asp Ser Arg
1190 1195 1200
Tyr Leu Ser Lys Ile Phe Arg Gly Asn Ala Val Thr Val
1205 1210 1215
<210> 112
<211> 1221
<212> PRT
<213> HPDH-MCRot
<400> 112
Met Phe Met Thr Arg Leu Asn Asp Lys Ile Ala Leu Ile Thr Gly Gly
1 5 10 15
Ala Gly Thr Ile Gly Glu Val Ile Thr Arg Arg Tyr Leu Glu Glu Gly
20 25 30
Ala Thr Val Val Met Ala Gly Arg Asn Arg Asp Lys Leu Asp Arg Tyr
35 40 45
Arg Glu Arg Leu Ile Thr Glu Phe His Ala Leu Pro Glu Arg Val Met
50 55 60
Val Val Arg Met Asp Gly Ser Ser Asn Ala Glu Val Arg Met Gly Ile
65 70 75 80
Ala Ala Val Val Ala His Phe Gly Arg Ile Asp Ile Leu Val Asn Asn
85 90 95
Ala Gly Ser Ala Gly Ala Arg Gln Arg Leu Pro Ala Ile Pro Leu Leu
100 105 110
Arg Ser Glu Leu Gln Ala Asp Glu Thr Glu Thr Leu Ala Asp Ser Ile
115 120 125
Gly Asn Leu Ile Gly Ile Thr Trp Asn Leu Ile Arg Ala Ala Ala Pro
130 135 140
Phe Met Pro Ala Gly Ser Ser Val Ile Asn Ile Ser Thr Ile Phe Ala
145 150 155 160
Arg Thr Asp Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ala Ala
165 170 175
Leu His Ala Leu Thr Leu Ala Ala Ala Thr Glu Leu Gly Glu Arg Gly
180 185 190
Ile Arg Val Asn Gln Ile Asn Pro Gly Pro Ile Asp Ser Asp Arg Ile
195 200 205
Arg Thr Val Phe Arg Arg Met Asp Glu Leu Lys Gly Val Pro Glu Gln
210 215 220
Ser Thr Ala Asp Gly Phe Phe Gln Met Met Arg Leu Arg Arg Pro Asn
225 230 235 240
Ala Glu Gly Asp Leu Val Lys Gly Phe Pro Lys Thr Leu Asp Val Ala
245 250 255
Asn Val Ala Val Phe Leu Gly Ser Ala Glu Ser Ala Ala Leu Ser Gly
260 265 270
Glu Thr Leu Asp Val Thr His Gly Met Ala Val Pro Thr Glu Ser Arg
275 280 285
Thr Thr Leu Thr Ser Arg Pro Gly Leu Arg Ala Val Asp Gly Ser Gly
290 295 300
His Thr Thr Leu Ile Cys Val Gly Asp Gln Ile Glu Glu Ala Ala Ala
305 310 315 320
Leu Thr Gly Val Leu Arg Ala Cys Gly Ala Glu Val Val Ile Gly Phe
325 330 335
Arg Ser Arg Ala Ala Ile Ala Arg Phe Asp His Leu Ile Glu Arg Gly
340 345 350
Arg His Leu Pro Ser Gln Glu His Val Ala Pro Val Leu Leu Tyr Leu
355 360 365
Asn Pro Thr Glu Pro Glu Ser Ile Asp Gln Ala Leu Arg Trp Met Ala
370 375 380
Thr Asn Leu Asp Leu Pro Thr Ser Val Ile Ile Leu Pro Ala Gln Arg
385 390 395 400
Gln Pro Leu Pro Pro Ser Val Val Arg Ala Ser Asp Glu Glu Val Ala
405 410 415
Tyr Phe Leu Arg Asp Glu Leu Ser Gly Met Ile Val Leu Ala Ser Arg
420 425 430
Leu Ala Arg Phe Trp Gln Gln Ala Thr Leu Ala Pro Gly Asn Ala Pro
435 440 445
Ile Gln Pro Arg Val Leu Phe Met Thr Asn Pro Asp Asp Gly Gln Gly
450 455 460
Asn Leu Tyr Ala Glu Ile Leu Arg Ala Gly Val Glu Gln Leu Cys Arg
465 470 475 480
Val Trp Arg His Glu Ser Gln Leu Asp Tyr Thr Arg Leu Ala Gln Met
485 490 495
Asp Ala His Pro Pro His Ile Arg Pro Val Trp Ala Asn Gln Leu Val
500 505 510
Arg Phe Ala Asn Asn Glu Gln Glu Asn Leu Glu Tyr Cys Cys Ala Trp
515 520 525
Val Ala Lys Ile Leu Leu Ser Glu Arg Thr Ile Glu Glu Leu Asn Leu
530 535 540
Tyr Leu Pro Arg Gln Ile Gly Ser Thr Thr Gly Ser Arg Gln Pro Ser
545 550 555 560
Phe Gly Trp Ala Glu Asn Leu Ile Gly Leu His Leu Gly Lys Thr Ala
565 570 575
Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Ser Gln Ile Ala Arg Leu
580 585 590
Leu Ala Leu Ser Gly Ala Arg Val Met Leu Cys Ala Arg Asp Glu Arg
595 600 605
Lys Leu Ile Gln Met Arg Asp Met Ile Ile Ala Glu Leu Thr Glu Val
610 615 620
Gly Tyr Asn Gln Val Glu Ser Arg Val Gln Ile Cys Ala Gly Cys Asp
625 630 635 640
Val Gly Glu Glu Glu Gln Leu Glu Ile Ala Val Gln Arg Thr Leu Asp
645 650 655
Leu Phe Gly His Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly
660 665 670
Ala Glu Glu Met Val Leu Asp Leu Pro Leu Glu Ala Trp Gln Arg Thr
675 680 685
Leu Arg Thr Asn Leu Ile Ser Asn Tyr Ser Leu Ile Arg Lys Leu Ala
690 695 700
Pro Gln Met Lys Ser Arg Gly Ser Gly Tyr Ile Leu Asn Val Ser Ser
705 710 715 720
Tyr Phe Gly Gly Glu Lys Tyr Ala Ala Ile Pro Tyr Pro Asn Arg Ala
725 730 735
Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Leu Gly Glu Ala Leu
740 745 750
Ala Arg Leu Leu Gly Pro Glu Val Gln Ile Asn Ala Met Ala Pro Gly
755 760 765
Pro Val Glu Gly Glu Arg Leu Arg Gly Ser Gly Asp Arg Pro Gly Leu
770 775 780
Phe Leu Arg Arg Gly Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Asp
785 790 795 800
Leu His Ala Thr Leu Ile Ala Ala Glu Arg Glu Thr Gln Val Gly Met
805 810 815
Arg Asp Leu Leu Ala Arg Leu Leu His Asn Asp Val Cys Ala Leu Ile
820 825 830
Asp Asp Pro Ala Ala Pro Thr His Leu Arg Ala Leu Ala Glu Arg Ile
835 840 845
Trp Glu Gln Ser Asp Pro Asn Ser Tyr Ala Arg Ala Phe Phe Met Asn
850 855 860
Ala Asn Ile Ala Thr Lys Leu Leu Ala Arg Leu Phe Asn Ala Asp Gln
865 870 875 880
Ile Asp Ala Gln Thr Phe His Thr Ser Gln Pro Asn Leu Pro Pro Glu
885 890 895
Pro Phe Phe Ala Arg Thr Gln Ile Asp Arg Glu Ala Arg Arg Val Arg
900 905 910
Asp Gly Val Met Ser Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe
915 920 925
Asp Val Ala Leu Ala Thr Val Tyr Tyr Leu Asn Asp Arg Ser Val Ser
930 935 940
Gly Glu Thr Phe His Pro Ser Gly Gly Leu Arg His Glu Arg Thr Pro
945 950 955 960
Thr Gly Ala Glu Leu Tyr Gly Ser Pro Ala Pro Gln Arg Leu Ala Ser
965 970 975
Leu Ala Gly Ser Thr Val Tyr Leu Ile Gly Glu Ser Met Ala Ala His
980 985 990
Leu Glu Ala Leu Ala Arg Ala Tyr Ile Glu Arg Tyr Ala Ala Thr Arg
995 1000 1005
Val Val Leu Ile Cys Ala Thr Pro Ala Gly Val Glu Arg Phe Ser
1010 1015 1020
His His Leu Ala Asp His Leu Ala Ser Gly Ala Leu Ala Ile Leu
1025 1030 1035
Ser Ala Glu Glu Gly Ile Glu Ala Ala Leu Ser Glu Ala Leu Arg
1040 1045 1050
Arg Phe Gly Pro Pro Gly Pro Val Val Ser Thr Pro Phe Gln Pro
1055 1060 1065
Leu Pro Ser Gln Pro Leu Ile Gly Arg Asn Asp Ser Asp Trp Ser
1070 1075 1080
Thr Val Leu Asp Val Ala Gly Phe Ser Ala Met Cys Glu Gln Gln
1085 1090 1095
Leu Thr His His Phe Arg Val Thr Arg Lys Leu Ser Leu Val Ala
1100 1105 1110
Gly Val Ser Leu Val Leu Val Thr Pro Glu Thr Asp Ser His Ser
1115 1120 1125
Ser Thr Glu Gln Phe Ala Leu Ala Asn Phe Val Lys Thr Thr Leu
1130 1135 1140
His Ala Phe Thr Ala Thr Val Gly Val Glu Cys Glu Arg Thr Ala
1145 1150 1155
His Arg Ile Leu Val Asn Gln Val Asp Leu Gly Arg Gln Ala Arg
1160 1165 1170
Ala Glu Glu Pro Arg Ser Pro Ala Glu Gln Ala Gln Glu Met Glu
1175 1180 1185
Arg Phe Ile Asp Ala Ile Met Leu Thr Thr Ala Pro Ile Pro Ala
1190 1195 1200
Glu Glu Asp Asn Arg Tyr Thr Gly Arg Ile Tyr Arg Gly Arg Ala
1205 1210 1215
Ile Thr Val
1220
<210> 113
<211> 1229
<212> PRT
<213> HPDH-MCRrc
<400> 113
Met Ser Thr Val Arg Arg Leu Glu Gly Lys Val Ala Leu Ile Thr Gly
1 5 10 15
Gly Ala Gly Asn Ile Gly Glu Val Ile Thr Arg Arg Phe Leu Ala Glu
20 25 30
Gly Ala Thr Val Val Ile Thr Gly Arg Asn Ala Glu Lys Leu Ala Val
35 40 45
Tyr Arg Arg Arg Leu Ile Asp Glu Glu Arg Val Ala Pro Glu Arg Val
50 55 60
Val Ala Leu Arg Met Asp Gly Ser Asp Ile Ala Gln Val Arg Ala Gly
65 70 75 80
Val Ala Gln Ile Val His Gly Gly Thr Asp Val Pro Ile Pro Leu His
85 90 95
Arg Ile Asp Ile Leu Val Asn Asn Ala Gly Ser Ala Gly Pro Arg Arg
100 105 110
Arg Leu Val Asp Ile Pro Leu Glu Pro Ser Glu Val Gln Pro Pro Asp
115 120 125
Ser Glu Thr Leu Ala Gln Ala Val Gly Asn Leu Val Gly Ile Thr Trp
130 135 140
Asn Leu Thr Arg Ala Ala Ala Pro His Met Pro Ser Gly Ser Ser Val
145 150 155 160
Ile Asn Ile Ser Thr Ile Phe Ser Arg Thr Asp Tyr Tyr Gly Arg Ile
165 170 175
Ala Tyr Val Ala Pro Lys Ala Ala Leu Asn Ala Leu Ser Asp Gly Leu
180 185 190
Ala Arg Glu Leu Gly Val Arg Gly Ile Arg Val Asn Thr Ile Tyr Pro
195 200 205
Gly Pro Ile Glu Ser Glu Arg Ile Tyr Thr Met Phe Gln Ala Met Asp
210 215 220
Ala Leu Lys Gly Gln Pro Glu Gly Asp Thr Ala Ser Gly Phe Leu Arg
225 230 235 240
Met Met Arg Leu Ser Arg Ile Asp Gln Asn Gly Glu Val Val Lys Arg
245 250 255
Phe Pro Ser Pro Val Asp Val Ala Asn Thr Ala Val Phe Leu Ala Ser
260 265 270
Asp Glu Ser Ala Ala Phe Thr Gly His Ala Phe Glu Val Thr His Gly
275 280 285
Met Glu Val Pro Thr Glu Ser Arg Thr Thr Phe Val Ser Arg Pro Gly
290 295 300
Leu Arg Ser Val Asp Ala Thr Gly Lys Val Ile Leu Ile Cys Ala Gly
305 310 315 320
Asp Gln Val Asp Asp Ala Val Ala Leu Ala Asp Thr Leu Arg Ser Cys
325 330 335
Arg Ala Thr Val Val Ile Gly Phe Arg Asp Pro Arg Ala Leu Glu Lys
340 345 350
Ala Ser Val Leu Leu Arg Glu Pro Arg His Ala Leu Ala Ala Asp Met
355 360 365
Tyr Gly Arg Pro Thr Met Thr Ala Glu Ala Arg Leu Val Arg Leu Asp
370 375 380
Pro Leu Asp Pro Arg Ala Ala Ala Gln Thr Leu Glu Gln Ile His Ala
385 390 395 400
Glu Leu Gly Ala Ile His His Ala Val Val Leu Pro Gly Gln Ser Arg
405 410 415
His Ala Pro Ser Ala Ser Leu Ile Glu Val Asp Asp Gln Val Val Glu
420 425 430
Arg Phe Leu His Gln Glu Leu Val Gly Thr Ile Ala Leu Ala Arg Glu
435 440 445
Leu Ala Arg Phe Trp Glu Glu Tyr Pro Ser Gly Ser Ser Met His Arg
450 455 460
Val Leu Phe Val Ser Asn Pro Asp Asp Gln Gln Gly Asn Gln Tyr Ser
465 470 475 480
His Ile Leu Arg Ala Ala Val Glu Gln Leu Val Arg Val Trp Arg His
485 490 495
Glu Ser Glu Tyr Asp Ser Val Asn Pro Ala His Gln Gln Glu Gly Gln
500 505 510
Ser Ser Ala Ala Val Trp Ala Asn Gln Leu Ile Arg Tyr Val Asn Asn
515 520 525
Glu Met Ala Asn Leu Asp Phe Thr Cys Ala Trp Val Ala Lys Leu Leu
530 535 540
Gly Ser Asp Arg Arg Ile Ala Glu Ile Asn Leu Tyr Leu Pro Glu Glu
545 550 555 560
Ile Val Gly Thr Ile Gly Val His Asn Pro Gly Phe Gly Trp Ala Glu
565 570 575
Ser Leu Phe Gly Leu His Met Gly Lys Val Ala Leu Ile Thr Gly Gly
580 585 590
Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu Leu Ala Leu Ser Gly
595 600 605
Ala His Val Met Leu Ala Ala Arg Asn Ala Asp Gln Leu Glu Gln Met
610 615 620
Arg Ala Ser Ile Val Arg Glu Val Arg Asp Ala Ser Tyr Pro Asp Ala
625 630 635 640
Glu Ser Arg Val Ala Ile Phe Pro Gly Ser Asp Val Ser Asp Ile Asp
645 650 655
Gly Leu Glu Arg Leu Val Asn His Thr Val Arg Val Phe Gly Lys Val
660 665 670
Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly Ala Glu Glu Met Val
675 680 685
Ile Asp Met Pro Val Asp Ala Trp Arg His Thr Leu Arg Ala Asn Leu
690 695 700
Ile Ser Asn Tyr Ala Leu Leu Arg Arg Leu Ala Pro Gln Met Lys Ala
705 710 715 720
Ala Gly Gly Ala Tyr Val Leu Asn Val Ser Ser Tyr Phe Gly Gly Glu
725 730 735
Lys Tyr Val Ala Ile Pro Tyr Pro Asn Arg Ser Asp Tyr Ala Val Ser
740 745 750
Lys Ala Gly Gln Arg Ala Met Val Glu Ser Leu Ala Arg Phe Leu Gly
755 760 765
Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly Pro Val Glu Gly Glu
770 775 780
Arg Leu Lys Gly Ala Gly Ser Arg Pro Gly Leu Phe Met Arg Arg Ala
785 790 795 800
Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu Val Phe Ala Ala Leu
805 810 815
Leu Ala Ala Arg His Glu Gly Ala Thr Ile Ala Asp Leu Leu Pro Asp
820 825 830
Leu Phe Ala Asn Asp Ile Gln Ser Ile Ala Asn Ser Ala Ala Met Pro
835 840 845
Ala Pro Leu Arg Arg Leu Ala Thr Met Leu Arg Glu Thr Ser Asp Ala
850 855 860
Gly Gly Ser Ala Gln Ser Tyr Leu Met Asn Ala Thr Ile Ala Arg Lys
865 870 875 880
Leu Leu Asn Arg Leu Glu Asn Gly Gly Tyr Ile Thr Leu His Asp Arg
885 890 895
Arg Ala Leu Thr Val Glu Pro Pro Glu Pro Phe Phe Thr Glu Ala Gln
900 905 910
Ile Glu Arg Glu Ala Ile Lys Val Arg Asp Gly Ile Leu Gly Met Leu
915 920 925
His Leu Gln Arg Met Pro Thr Glu Phe Asp Val Ala Leu Ala Thr Val
930 935 940
Phe Tyr Leu Ala Asp Arg Asn Val Thr Gly Glu Thr Phe His Pro Ser
945 950 955 960
Gly Gly Leu Arg Phe Glu Arg Thr Val Thr Glu Gly Glu Leu Phe Gly
965 970 975
Lys Pro Gly Gln Gln Arg Leu Glu Arg Leu Lys Gly Ser Val Val Tyr
980 985 990
Leu Ile Gly Glu His Leu Arg Gln His Leu Val Leu Leu Ala Arg Thr
995 1000 1005
Phe Leu Asp Glu Ile His Val Ala Arg Val Val Leu Leu Thr Glu
1010 1015 1020
Thr Thr Gln Ala Ala Thr Asp Leu Ala Ala Glu Leu Ser Asp Tyr
1025 1030 1035
Glu Ala Ala Gly Arg Phe Val Val Ile Pro Thr Cys Gly Asp Ile
1040 1045 1050
Glu Gly Gly Ile Asp Arg Ala Met Ala Glu Tyr Gly Arg Pro Gly
1055 1060 1065
Pro Val Ile Ser Thr Pro Phe Arg Pro Leu Pro Asp Arg Ala Leu
1070 1075 1080
Ser Ala Arg Asn Gly Asp Trp Ser Ser Val Leu Thr Thr Ala Glu
1085 1090 1095
Phe Glu Glu Leu Val Glu Gln Gln Ile Thr His His Phe Arg Val
1100 1105 1110
Ala Arg Lys Ala Gly Leu Ile Glu Gly Ala Asn Val Thr Leu Val
1115 1120 1125
Thr Pro Pro Thr Ser Ala Arg Ser Thr Ser Glu Glu Phe Ala Leu
1130 1135 1140
Ala Asn Phe Val Lys Thr Thr Leu His Ala Leu Thr Ala Thr Ala
1145 1150 1155
Gly Ala Glu Ser Glu Arg Thr Val Pro His Val Pro Val Asn Gln
1160 1165 1170
Val Asp Leu Thr Arg Arg Ala Arg Ser Glu Glu Pro Arg Thr Pro
1175 1180 1185
Ser Glu Glu Glu Glu Glu Leu Gln Arg Phe Val Asn Ala Val Leu
1190 1195 1200
Leu Thr Ser Ala Pro Leu Pro Thr Pro Leu Glu Ser Arg Tyr Arg
1205 1210 1215
Ala Arg Ile Tyr Arg Gly Asn Ala Ile Thr Val
1220 1225
<210> 114
<211> 1204
<212> PRT
<213> HPDH-MCRsl
<400> 114
Met Asn Ala Met Gly Lys Thr Glu Thr Gln Gly Arg Leu Ala Gly Lys
1 5 10 15
Val Ala Leu Ile Thr Gly Ala Ala Gly Asn Leu Gly Gly Ser Ile Val
20 25 30
Arg Arg Tyr Leu Ala Glu Gly Ala Thr Val Val Met Ser Gly Arg Asp
35 40 45
Ala Ala Arg Thr Gln Ala Ala Leu Asp Ala Ala Val Ala Asp Val Gly
50 55 60
Gly Thr Ala Ser Ile Val Thr Met Asp Gly Ala Asn Ile Glu Ser Val
65 70 75 80
Arg Ala Gly Ile Ala Asp Val Val Ala Arg His Gly Arg Ile Asp Val
85 90 95
Leu Val Asn Asn Ala Gly Ser Ala Gly Pro Lys Gln Val Leu Gly Asp
100 105 110
Leu Pro Leu Leu Pro Glu Glu Leu Glu Gly Thr Pro Asp Ser Glu Thr
115 120 125
Val Gly Asp Ala Ala Arg Asn Ile Met Val Val Ala Trp Asn Leu Val
130 135 140
Arg Ala Ala Ala Ser Ala Met Gly Glu Gly Gly Ser Ile Ile Asn Val
145 150 155 160
Ser Thr Ile Phe Ser Arg Thr Asp Tyr Phe Gly Arg Ala Ala Tyr Thr
165 170 175
Val Pro Lys Ala Ala Leu Asn Ile Leu Ser Lys Gly Ile Ala Arg Glu
180 185 190
Leu Gly Thr Arg Gly Ile Arg Val Asn Gln Leu Tyr Pro Gly Pro Ile
195 200 205
Ala Ser Glu Arg Ile Arg Thr Val Phe Ala Ala Met Asp Lys Leu Lys
210 215 220
Gly Asp Asp Ala Gly Ser Thr Ala Asn His Phe Phe Asn Leu Met Ala
225 230 235 240
Leu Glu Arg Ser Val Gly Glu Asp Ala Lys Ala Lys Thr Phe Pro Thr
245 250 255
Pro Ala Asp Ile Ala Asn Thr Cys Val Phe Leu Gly Ser Asp Glu Ser
260 265 270
Ala Ala Phe Ala Ala His Asp Phe Glu Val Thr His Gly Met Ala Val
275 280 285
Pro Leu Glu Ser Lys Ser Glu Phe Leu His Arg Pro Asp Leu Arg Ala
290 295 300
Ile Asp Gly Asp Gly His Gly Ile Leu Ile Ser Ala Gly Asn Gln Leu
305 310 315 320
Asp Asp Ala Leu Ala Met Ala Gly Leu Phe Ala Ala Gln Gly Ser Lys
325 330 335
Val Leu Leu Gly Phe His Ala Gln Ala Ala Leu Asp Asp Ala Ala Glu
340 345 350
Arg Leu Pro Ala Gly Val Lys Ala Val Arg Phe Pro Arg His Asp His
355 360 365
Met Ala Met Asp Ala Glu Leu Ala Arg Phe Thr Ala His Val Ala Ala
370 375 380
Ile Asp Gly Ala Ile Ile Leu Pro Thr Arg Gly Pro Gly Tyr Phe His
385 390 395 400
Gly Ala Leu Asn Glu Ala Ser Asp Ala Ala Val Glu Arg Phe Val Asp
405 410 415
Asp Glu Leu Glu Gly Ala Ile Ala Val Ala Arg Thr Leu Asp Arg Tyr
420 425 430
Trp Gln Gln Gly Gln Asp Leu Pro Gly Asp Pro Arg Val Leu Phe Ile
435 440 445
Thr Asn Pro Asp Asp Gly Ala Gly Asn Gly Trp Ala Asp Val Gly Arg
450 455 460
Ala Ala Ile Glu Glu Leu Leu Arg Val Trp Arg Asp Glu Ala Arg Val
465 470 475 480
Ala Ala Thr Gln Gly Lys Thr Pro Arg Pro Val Trp Gly Asn Gln Ile
485 490 495
Val Arg Phe Gly Asn Asn Glu Pro Glu Asn Arg Asp Phe Ala Ala Gly
500 505 510
His Ala Ala Arg Leu Leu Leu Gly Gly Arg Gln Ile Glu Pro Ile Asn
515 520 525
Leu Tyr Ile Pro Ala Ser Ile Thr Lys Ala Thr Gly Ala Arg Arg Ala
530 535 540
Leu Ala Gly Thr Ala Glu Asn Ile Thr Gly Leu His Leu Gly Lys Val
545 550 555 560
Ala Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Ala Leu Ala Arg
565 570 575
Leu Leu Ala Leu Ala Gly Ala Lys Val Met Met Val Ala Arg Arg Glu
580 585 590
Ser Glu Leu Asp Ala Ala Arg Glu Arg Ile Val Gly Glu Leu Gln Asp
595 600 605
Ile Gly Phe Ala Gly Val Glu Arg Arg Val Leu Thr Leu Ala Gly Ile
610 615 620
Asp Val Ala Asp Met Lys Ser Leu Lys His Ala Val Asp Glu Thr Val
625 630 635 640
Arg Ala Phe Gly Arg Ile Asp Tyr Leu Ile Asn Asn Ala Gly Val Ser
645 650 655
Gly Ala Glu Glu Met Ala Val Asp Met Gly Val Glu Asn Phe Arg His
660 665 670
Thr Gln Phe Ala Asn Leu Ile Ser Asn Tyr Ala Leu Met His Met Val
675 680 685
Val Pro Met Met Arg Lys Gln Gly Ser Gly Tyr Ile Leu Asn Val Ser
690 695 700
Ser Tyr Phe Gly Gly Glu Lys Tyr Leu Ala Val Ser Tyr Pro Asn Arg
705 710 715 720
Thr Asp Tyr Ala Val Ser Lys Ser Gly Gln Arg Ala Met Val Glu Ser
725 730 735
Met Ala Arg His Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro
740 745 750
Gly Pro Val Asp Gly Asp Arg Leu Ala Gly Leu Gly Gly Lys Pro Gly
755 760 765
Leu Phe Glu Arg Arg Gly Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn
770 775 780
Ala Val His Ser Ala Val Ile Lys Ser Val Arg Ala Gly Gly Ser Val
785 790 795 800
Ala Gln Ile Leu Phe Arg Leu Ser Arg Asn Asp Ser Val Gln Leu Ser
805 810 815
His Asp Lys Ser Asn Pro Gln Pro Leu Arg Asp Leu Ala Leu Ala Cys
820 825 830
Ala Arg Glu Gly Asp Glu Ser Cys Cys Trp Asp Arg Phe Ile Leu Thr
835 840 845
Pro Val Leu Ala Ala Lys Leu Leu Lys Arg Leu Arg Leu Gly Gly Trp
850 855 860
Leu Ile Gly Thr Asp Trp Gln Ser Arg Asp Glu Ser Asp Trp Leu Leu
865 870 875 880
Arg Val Pro Pro Asp Asp Met Pro Trp Leu Pro Val Ala Gln Ile Gly
885 890 895
Arg Glu Ala Ala Lys Val Arg Gly Gly Val Leu Ser Gln Leu His Leu
900 905 910
Gly Lys Met Pro Thr Glu Thr Glu Val Ala Gln Ala Thr Val Phe Phe
915 920 925
Leu Ala Asp Arg Ala Val Ser Gly Glu Thr Phe Met Pro Ser Gly Gly
930 935 940
Leu Asn Val Glu Arg Ser Ala Thr Glu Arg Glu Leu Phe Gly Ser Pro
945 950 955 960
Lys Ala Glu Arg Leu Ala Gln Met Gly Gly Lys Thr Val Trp Leu Val
965 970 975
Gly Glu His Leu Ala Asp Tyr Leu Gly Ala Ala Ala Gln Gly Phe Leu
980 985 990
Asn Cys Asp Val Ala Arg Val Val Leu Ile Thr Ala Ser Ala Ala Gly
995 1000 1005
Ala Thr Ala Ile Lys Gly His Ile Gly Ser Gly Ala Asp Arg Val
1010 1015 1020
Glu Ser Leu Leu Ile Asp Gly Glu Ile Glu Thr Ala Leu Asp Ala
1025 1030 1035
Ala Leu Ala Thr Trp Gly His Pro Thr Thr Ile Val Ser Thr Pro
1040 1045 1050
Leu Lys Ala Leu Pro Asp Gln Leu Phe Asp Ala Asp Asn Pro Leu
1055 1060 1065
Asp Gly Asp Gly Phe Ala Asp Val Val Glu His Asn Leu Thr His
1070 1075 1080
His Phe Arg Ile Ala Arg Lys Val Ser Leu Phe Asp Glu Cys Gln
1085 1090 1095
Leu Val Leu Val Ser Pro Asp Val Pro Met Gly Glu Gly Ala Lys
1100 1105 1110
Gly Ala Phe Ala Leu Ala Asn Phe Val Lys Thr Thr Leu His Ala
1115 1120 1125
Phe Thr Ala Thr Ile Ala Val Glu Asn Glu Arg Leu Val His Glu
1130 1135 1140
Val Ala Val Asn Gln Ile Asn Leu Thr Arg Arg Val Arg Ser Glu
1145 1150 1155
Glu Pro Lys Asn Ala Ala Glu His Glu Glu Glu Val Arg Arg Phe
1160 1165 1170
Ala Arg Ala Val Leu Leu Ala Gly Ala Pro Leu Pro Asp Ala Glu
1175 1180 1185
Asp Ser Arg Tyr Arg Ser Arg Ile Tyr Arg Gly Met Ser Leu Thr
1190 1195 1200
Val
<210> 115
<211> 1220
<212> PRT
<213> HPDH-MCRca_(变体_3)
<400> 115
Met Ser Gly Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly
1 5 10 15
Gly Ala Gly Asn Ile Gly Ser Glu Leu Thr Arg Arg Phe Leu Ala Glu
20 25 30
Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Arg Ala Lys Leu Thr Ala
35 40 45
Leu Ala Glu Arg Met Gln Ala Glu Ala Gly Val Pro Ala Lys Arg Ile
50 55 60
Asp Leu Glu Val Met Asp Gly Ser Asp Pro Val Ala Val Arg Ala Gly
65 70 75 80
Ile Glu Ala Ile Val Ala Arg His Gly Gln Ile Asp Ile Leu Val Asn
85 90 95
Asn Ala Gly Ser Ala Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu
100 105 110
Thr Glu Ala Glu Leu Gly Pro Gly Ala Glu Glu Thr Leu His Ala Ser
115 120 125
Ile Ala Asn Leu Leu Gly Met Gly Trp His Leu Met Arg Ile Ala Ala
130 135 140
Pro His Met Pro Val Gly Ser Ala Val Ile Asn Val Ser Thr Ile Phe
145 150 155 160
Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Thr Pro Lys Ala
165 170 175
Ala Leu Asn Ala Leu Ser Gln Leu Ala Ala Arg Glu Leu Gly Ala Arg
180 185 190
Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Asp Arg
195 200 205
Ile Arg Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu
210 215 220
Gly Asp Thr Ala His His Phe Leu Asn Thr Met Arg Leu Cys Arg Ala
225 230 235 240
Asn Asp Gln Gly Ala Leu Glu Arg Arg Phe Pro Ser Val Gly Asp Val
245 250 255
Ala Asp Ala Ala Val Phe Leu Ala Ser Ala Glu Ser Ala Ala Leu Ser
260 265 270
Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Ala Cys Ser
275 280 285
Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Ser
290 295 300
Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met
305 310 315 320
Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly
325 330 335
Phe Arg Ser Ala Ala Ala Leu Ala Gln Phe Glu Gln Ala Val Asn Glu
340 345 350
Ser Arg Arg Leu Ala Gly Ala Asp Phe Thr Pro Pro Ile Ala Leu Pro
355 360 365
Leu Asp Pro Arg Asp Pro Ala Thr Ile Asp Ala Val Phe Asp Trp Gly
370 375 380
Ala Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala
385 390 395 400
Thr Ser His Glu Pro Ala Pro Cys Val Ile Glu Val Asp Asp Glu Arg
405 410 415
Val Leu Asn Phe Leu Ala Asp Glu Ile Thr Gly Thr Ile Val Ile Ala
420 425 430
Ser Arg Leu Ala Arg Tyr Trp Gln Ser Gln Arg Leu Thr Pro Gly Ala
435 440 445
Arg Ala Arg Gly Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Asp Gln
450 455 460
Asn Gly Asn Val Tyr Gly Arg Ile Gln Ser Ala Ala Ile Gly Gln Leu
465 470 475 480
Ile Arg Val Trp Arg His Glu Ala Glu Leu Asp Tyr Gln Arg Ala Ser
485 490 495
Ala Ala Gly Asp His Val Leu Pro Pro Val Trp Ala Asn Gln Ile Val
500 505 510
Arg Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp
515 520 525
Thr Ala Gln Leu Leu His Ser Gln Arg His Ile Asn Glu Ile Thr Leu
530 535 540
Asn Ile Pro Ala Asn Ile Ser Ala Thr Thr Gly Ala Arg Ser Ala Ser
545 550 555 560
Val Gly Trp Ala Glu Ser Leu Ile Gly Leu His Leu Gly Lys Val Ala
565 570 575
Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu
580 585 590
Leu Ala Leu Ser Gly Ala Arg Val Met Leu Ala Asp Ile Gln Glu Asp
595 600 605
Lys Leu Glu Gln Met Gln Ala Met Ile Gln Ser Glu Leu Ala Glu Val
610 615 620
Gly Tyr Thr Asp Val Glu Asp Arg Val His Ile Ala Pro Gly Cys Asp
625 630 635 640
Val Ser Ser Glu Ala Gln Leu Ala Asp Leu Val Glu Arg Thr Leu Ser
645 650 655
Ala Phe Gly Thr Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly
660 665 670
Val Glu Glu Met Val Ile Asp Met Pro Val Glu Gly Trp Arg His Thr
675 680 685
Leu Phe Ala Asn Leu Ile Ser Asn Tyr Ser Leu Met Arg Lys Leu Ala
690 695 700
Pro Leu Met Lys Lys Gln Gly Ser Gly Tyr Ile Leu Asn Val Ser Ser
705 710 715 720
Tyr Phe Gly Gly Glu Lys Asp Ala Ala Ile Pro Tyr Pro Asn Arg Ala
725 730 735
Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Ala Glu Val Phe
740 745 750
Ala Arg Phe Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly
755 760 765
Pro Val Glu Gly Asp Arg Leu Arg Gly Thr Gly Glu Arg Pro Gly Leu
770 775 780
Phe Ala Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu
785 790 795 800
Leu His Ala Ala Leu Ile Ala Ala Ala Arg Thr Asp Glu Arg Ser Met
805 810 815
His Glu Leu Val Glu Leu Leu Leu Pro Asn Asp Val Ala Ala Leu Glu
820 825 830
Gln Asn Pro Ala Ala Pro Thr Ala Leu Arg Glu Leu Ala Arg Arg Phe
835 840 845
Arg Ser Glu Gly Asp Pro Ala Ala Ser Ser Ser Ser Ala Leu Leu Asn
850 855 860
Arg Ser Ile Ala Ala Lys Leu Leu Ala Arg Leu His Asn Gly Gly Tyr
865 870 875 880
Val Leu Pro Ala Asp Ile Phe Ala Asn Leu Pro Asn Pro Pro Asp Pro
885 890 895
Phe Phe Thr Arg Ala Gln Ile Asp Arg Glu Ala Arg Lys Val Arg Asp
900 905 910
Gly Ile Met Gly Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe Asp
915 920 925
Val Ala Met Ala Thr Val Tyr Tyr Leu Ala Asp Arg Asn Val Ser Gly
930 935 940
Glu Thr Phe His Pro Ser Gly Gly Leu Arg Tyr Glu Arg Thr Pro Thr
945 950 955 960
Gly Gly Glu Leu Phe Gly Leu Pro Ser Pro Glu Arg Leu Ala Glu Leu
965 970 975
Val Gly Ser Thr Val Tyr Leu Ile Gly Glu His Leu Thr Glu His Leu
980 985 990
Asn Leu Leu Ala Arg Ala Tyr Leu Glu Arg Tyr Gly Ala Arg Gln Val
995 1000 1005
Val Met Ile Val Glu Thr Glu Thr Gly Ala Glu Thr Met Arg Arg
1010 1015 1020
Leu Leu His Asp His Val Glu Ala Gly Arg Leu Met Thr Ile Val
1025 1030 1035
Ala Gly Asp Gln Ile Glu Ala Ala Ile Asp Gln Ala Ile Thr Arg
1040 1045 1050
Tyr Gly Arg Pro Gly Pro Val Val Cys Thr Pro Phe Arg Pro Leu
1055 1060 1065
Pro Thr Val Pro Leu Val Gly Arg Lys Asp Ser Asp Trp Ser Thr
1070 1075 1080
Val Leu Ser Glu Ala Glu Phe Ala Glu Leu Cys Glu His Gln Leu
1085 1090 1095
Thr His His Phe Arg Val Ala Arg Lys Ile Ala Leu Ser Asp Gly
1100 1105 1110
Ala Ser Leu Ala Leu Val Thr Pro Glu Thr Thr Ala Thr Ser Thr
1115 1120 1125
Thr Glu Gln Phe Ala Leu Ala Asn Phe Ile Lys Thr Thr Leu His
1130 1135 1140
Ala Phe Thr Ala Thr Ile Gly Val Glu Ser Glu Arg Thr Ala Gln
1145 1150 1155
Arg Ile Leu Ile Asn Gln Val Asp Leu Thr Arg Arg Ala Arg Ala
1160 1165 1170
Glu Glu Pro Arg Asp Pro His Glu Arg Gln Gln Glu Leu Glu Arg
1175 1180 1185
Phe Ile Glu Ala Val Leu Leu Val Thr Ala Pro Leu Pro Pro Glu
1190 1195 1200
Ala Asp Thr Arg Tyr Ala Gly Arg Ile His Arg Gly Arg Ala Ile
1205 1210 1215
Thr Val
1220
<210> 116
<211> 1220
<212> PRT
<213> HPDH-MCRca_(变体_6)
<400> 116
Met Ser Gly Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly
1 5 10 15
Gly Ala Gly Asn Ile Gly Ser Glu Leu Thr Arg Arg Phe Leu Ala Glu
20 25 30
Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Arg Ala Lys Leu Thr Ala
35 40 45
Leu Ala Glu Arg Met Gln Ala Glu Ala Gly Val Pro Ala Lys Arg Ile
50 55 60
Asp Leu Glu Val Met Asp Gly Ser Asp Pro Val Ala Val Arg Ala Gly
65 70 75 80
Ile Glu Ala Ile Val Ala Arg His Gly Gln Ile Asp Ile Leu Val Asn
85 90 95
Asn Ala Gly Ser Ala Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu
100 105 110
Thr Glu Ala Glu Leu Gly Pro Gly Ala Glu Glu Thr Leu His Ala Ser
115 120 125
Ile Ala Asn Leu Leu Gly Met Gly Trp His Leu Met Arg Ile Ala Ala
130 135 140
Pro His Met Pro Val Gly Ser Ala Val Ile Asn Val Ser Thr Ile Phe
145 150 155 160
Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Thr Pro Lys Ala
165 170 175
Ala Leu Asn Ala Leu Ser Gln Leu Ala Ala Arg Glu Leu Gly Ala Arg
180 185 190
Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Asp Arg
195 200 205
Ile Arg Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu
210 215 220
Gly Asp Thr Ala His His Phe Leu Asn Thr Met Arg Leu Cys Arg Ala
225 230 235 240
Asn Asp Gln Gly Ala Leu Glu Arg Arg Phe Pro Ser Val Gly Asp Val
245 250 255
Ala Asp Ala Ala Val Phe Leu Ala Ser Ala Glu Ser Ala Ala Leu Ser
260 265 270
Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Ala Cys Ser
275 280 285
Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Ser
290 295 300
Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met
305 310 315 320
Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly
325 330 335
Phe Arg Ser Ala Ala Ala Leu Ala Gln Phe Glu Gln Ala Val Asn Glu
340 345 350
Ser Arg Arg Leu Ala Gly Ala Asp Phe Thr Pro Pro Ile Ala Leu Pro
355 360 365
Leu Asp Pro Arg Asp Pro Ala Thr Ile Asp Ala Val Phe Asp Trp Gly
370 375 380
Ala Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala
385 390 395 400
Thr Ser His Glu Pro Ala Pro Cys Val Ile Glu Val Asp Asp Glu Arg
405 410 415
Val Leu Asn Phe Leu Ala Asp Glu Ile Thr Gly Thr Ile Val Ile Ala
420 425 430
Ser Arg Leu Ala Arg Tyr Trp Gln Ser Gln Arg Leu Thr Pro Gly Ala
435 440 445
Arg Ala Arg Gly Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Asp Gln
450 455 460
Asn Gly Asn Val Tyr Gly Arg Ile Gln Ser Ala Ala Ile Gly Gln Leu
465 470 475 480
Ile Arg Val Trp Arg His Glu Ala Glu Leu Asp Tyr Gln Arg Ala Ser
485 490 495
Ala Ala Gly Asp His Val Leu Pro Pro Val Trp Ala Asn Gln Ile Val
500 505 510
Arg Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp
515 520 525
Thr Ala Gln Leu Leu His Ser Gln Arg His Ile Asn Glu Ile Thr Leu
530 535 540
Asn Ile Pro Ala Asn Ile Ser Ala Thr Thr Gly Ala Arg Ser Ala Ser
545 550 555 560
Val Gly Trp Ala Glu Ser Leu Ile Gly Leu His Leu Gly Lys Val Ala
565 570 575
Leu Ile Thr Gly Gly Ser Ala Gly Ile Gly Gly Gln Ile Gly Arg Leu
580 585 590
Leu Ala Leu Ser Gly Ala Arg Val Met Leu Ala Asp Val Asp Thr Arg
595 600 605
Lys Leu Glu Gln Met Gln Ala Met Ile Gln Ser Glu Leu Ala Glu Val
610 615 620
Gly Tyr Thr Asp Val Glu Asp Arg Val His Ile Ala Pro Gly Cys Asp
625 630 635 640
Val Ser Ser Glu Ala Gln Leu Ala Asp Leu Val Glu Arg Thr Leu Ser
645 650 655
Ala Phe Gly Thr Val Asp Tyr Leu Ile Asn Asn Ala Gly Ile Ala Gly
660 665 670
Val Glu Glu Met Val Ile Asp Met Pro Val Glu Gly Trp Arg His Thr
675 680 685
Leu Phe Ala Asn Leu Ile Ser Asn Tyr Ser Leu Met Arg Lys Leu Ala
690 695 700
Pro Leu Met Lys Lys Gln Gly Ser Gly Tyr Ile Leu Asn Val Ser Ser
705 710 715 720
Tyr Phe Gly Gly Glu Lys Asp Ala Ala Ile Pro Tyr Pro Asn Arg Ala
725 730 735
Asp Tyr Ala Val Ser Lys Ala Gly Gln Arg Ala Met Ala Glu Val Phe
740 745 750
Ala Arg Phe Leu Gly Pro Glu Ile Gln Ile Asn Ala Ile Ala Pro Gly
755 760 765
Pro Val Glu Gly Asp Arg Leu Arg Gly Thr Gly Glu Arg Gly Gly Leu
770 775 780
Phe Gly Arg Arg Ala Arg Leu Ile Leu Glu Asn Lys Arg Leu Asn Glu
785 790 795 800
Leu His Ala Ala Leu Ile Ala Ala Ala Arg Thr Asp Glu Arg Ser Met
805 810 815
His Glu Leu Val Glu Leu Leu Leu Pro Asn Asp Val Ala Ala Leu Glu
820 825 830
Gln Asn Pro Ala Ala Pro Thr Ala Leu Arg Glu Leu Ala Arg Arg Phe
835 840 845
Arg Ser Glu Gly Asp Pro Ala Ala Ser Ser Ser Ser Ala Leu Leu Asn
850 855 860
Arg Ser Ile Ala Ala Lys Leu Leu Ala Arg Leu His Asn Gly Gly Tyr
865 870 875 880
Val Leu Pro Ala Asp Ile Phe Ala Asn Leu Pro Asn Pro Pro Asp Pro
885 890 895
Phe Phe Thr Arg Ala Gln Ile Asp Arg Glu Ala Arg Lys Val Arg Asp
900 905 910
Gly Ile Met Gly Met Leu Tyr Leu Gln Arg Met Pro Thr Glu Phe Asp
915 920 925
Val Ala Met Ala Thr Val Tyr Tyr Leu Ala Asp Arg Asn Val Ser Gly
930 935 940
Glu Thr Phe His Pro Ser Gly Gly Leu Arg Tyr Glu Arg Thr Pro Thr
945 950 955 960
Gly Gly Glu Leu Phe Gly Leu Pro Ser Pro Glu Arg Leu Ala Glu Leu
965 970 975
Val Gly Ser Thr Val Tyr Leu Ile Gly Glu His Leu Thr Glu His Leu
980 985 990
Asn Leu Leu Ala Arg Ala Tyr Leu Glu Arg Tyr Gly Ala Arg Gln Val
995 1000 1005
Val Met Ile Val Glu Thr Glu Thr Gly Ala Glu Thr Met Arg Arg
1010 1015 1020
Leu Leu His Asp His Val Glu Ala Gly Arg Leu Met Thr Ile Val
1025 1030 1035
Ala Gly Asp Gln Ile Glu Ala Ala Ile Asp Gln Ala Ile Thr Arg
1040 1045 1050
Tyr Gly Arg Pro Gly Pro Val Val Cys Thr Pro Phe Arg Pro Leu
1055 1060 1065
Pro Thr Val Pro Leu Val Gly Arg Lys Asp Ser Asp Trp Ser Thr
1070 1075 1080
Val Leu Ser Glu Ala Glu Phe Ala Glu Leu Cys Glu His Gln Leu
1085 1090 1095
Thr His His Phe Arg Val Ala Arg Lys Ile Ala Leu Ser Asp Gly
1100 1105 1110
Ala Ser Leu Ala Leu Val Thr Pro Glu Thr Thr Ala Thr Ser Thr
1115 1120 1125
Thr Glu Gln Phe Ala Leu Ala Asn Phe Ile Lys Thr Thr Leu His
1130 1135 1140
Ala Phe Thr Ala Thr Ile Gly Val Glu Ser Glu Arg Thr Ala Gln
1145 1150 1155
Arg Ile Leu Ile Asn Gln Val Asp Leu Thr Arg Arg Ala Arg Ala
1160 1165 1170
Glu Glu Pro Arg Asp Pro His Glu Arg Gln Gln Glu Leu Glu Arg
1175 1180 1185
Phe Ile Glu Ala Val Leu Leu Val Thr Ala Pro Leu Pro Pro Glu
1190 1195 1200
Ala Asp Thr Arg Tyr Ala Gly Arg Ile His Arg Gly Arg Ala Ile
1205 1210 1215
Thr Val
1220
<210> 117
<211> 258
<212> PRT
<213> BDHcm
<400> 117
Met Val Asn Leu Gln Gly Lys Thr Ala Leu Val Thr Gly Ser Thr Ser
1 5 10 15
Gly Ile Gly Leu Gly Ile Ala Glu Ala Leu Gly Arg Ala Gly Ala Asn
20 25 30
Ile Val Leu Asn Gly Phe Gly Asp Val Asp Ala Ala Leu Ala Thr Ile
35 40 45
Ala Ala Thr Gly Ala Gln Ser Ala His His Pro Ala Asp Met Arg Lys
50 55 60
Pro Asp Glu Ile Glu Ala Met Ile Ala Met Ala Arg Glu Arg Phe Gly
65 70 75 80
Thr Val Asp Val Leu Val Asn Asn Ala Gly Ile Gln His Val Ala Gln
85 90 95
Val Glu Asp Phe Pro Ala Gln Lys Trp Asp Asp Ile Leu Ala Ile Asn
100 105 110
Leu Thr Ser Ser Phe His Thr Thr Arg His Val Ile Pro Ala Met Arg
115 120 125
Ala Arg Asn Trp Gly Arg Ile Val Asn Ile Ala Ser Val His Gly Leu
130 135 140
Val Gly Ser Ala Gly Lys Ser Ala Tyr Val Ala Ala Lys His Gly Leu
145 150 155 160
Val Gly Leu Thr Lys Val Thr Ala Leu Glu Thr Ala Gly Thr Gly Ile
165 170 175
Thr Cys Asn Ala Ile Cys Pro Gly Phe Val Leu Thr Pro Leu Val Ala
180 185 190
Ala Gln Ile Asp Ala Ile Ala Lys Arg Asp Gly Ile Ser Met Glu Ala
195 200 205
Ala Arg Ala Arg Leu Leu Ser Asp Lys Gln Pro Ser Gly Gln Phe Val
210 215 220
Thr Pro Glu Gln Leu Gly Asn Leu Val Leu Met Leu Cys Ser Pro Phe
225 230 235 240
Gly Asp Gln Val Arg Gly Ala Ala Trp Ala Met Asp Gly Gly Trp Thr
245 250 255
Ala Gln
<210> 118
<211> 256
<212> PRT
<213> BDHkp
<400> 118
Met Asn Leu His Gly Lys Thr Ala Leu Val Thr Gly Ser Thr Ser Gly
1 5 10 15
Ile Gly Leu Gly Ile Ala Lys Val Leu Ala Gln Ala Gly Ala Gln Leu
20 25 30
Val Leu Asn Gly Phe Gly Asp Ser Ser His Ala Arg Ala Glu Val Ala
35 40 45
Ala Leu Gly Lys Ile Pro Gly Tyr His Asp Ala Asp Leu Arg Asp Val
50 55 60
Gly Gln Ile Glu Ala Met Met His Tyr Ala Glu Ser Thr Phe Gly Gly
65 70 75 80
Val Asp Ile Val Ile Asn Asn Ala Gly Ile Gln His Val Ala Pro Val
85 90 95
Glu Gln Phe Pro Val Asp Lys Trp Asn Asp Ile Leu Ala Ile Asn Leu
100 105 110
Ser Ser Val Phe His Thr Thr Arg Leu Ala Leu Pro Gly Met Arg Gln
115 120 125
Arg Asn Trp Gly Arg Ile Ile Asn Ile Ala Ser Val His Gly Leu Val
130 135 140
Ala Ser Lys Glu Lys Ser Ala Tyr Val Ala Ala Lys His Ala Val Val
145 150 155 160
Gly Leu Thr Lys Thr Val Ala Leu Glu Thr Ala Arg Ser Gly Ile Thr
165 170 175
Cys Asn Ala Ile Cys Pro Gly Trp Val Leu Thr Pro Leu Val Gln Gln
180 185 190
Gln Ile Asp Lys Arg Ile Ala Glu Gly Val Asp Pro Glu Gln Ala Ser
195 200 205
Ala Gln Leu Leu Ala Glu Lys Gln Pro Ser Gly Glu Phe Val Thr Pro
210 215 220
Gln Gln Leu Gly Glu Met Ala Leu Phe Leu Cys Ser Asp Ala Ala Ala
225 230 235 240
Gln Val Arg Gly Ala Ala Trp Asn Met Asp Gly Gly Trp Val Ala Gln
245 250 255
<210> 119
<211> 370
<212> PRT
<213> HBDHos
<400> 119
Met Gln Leu Phe Lys Leu Lys Thr Arg Leu Ser Lys Phe Asp His Phe
1 5 10 15
Ala Asp Phe Ala Arg Glu Phe Lys Leu Ser Glu His Asp Leu Val Ile
20 25 30
Thr Asn Glu Phe Leu Tyr Glu Pro Phe Met Lys Ser Leu Asn Leu Pro
35 40 45
Cys His Phe Ile Met Gln Glu Lys Phe Gly Leu Gly Glu Pro Ser Asp
50 55 60
Lys Met Met Asn Thr Ile Leu Lys Glu Val Lys Gly Val Gly Phe Asp
65 70 75 80
Arg Val Ile Ala Val Gly Gly Gly Thr Val Ile Asp Ile Ser Lys Leu
85 90 95
Phe Val Leu Lys Gly Val Glu Asp Val Thr Glu Ala Phe Glu Arg Lys
100 105 110
Ile Pro Ile Val Lys Glu Lys Gln Leu Val Ile Leu Pro Thr Thr Cys
115 120 125
Gly Thr Gly Ser Glu Val Thr Asn Ile Ser Ile Ala Glu Ile Thr Ser
130 135 140
Lys His Thr Lys Met Gly Leu Ala Asp Asp Ala Ile Val Ala Asp Asp
145 150 155 160
Ala Val Leu Val Pro Glu Leu Leu Lys Gly Leu Pro Phe Lys Phe Tyr
165 170 175
Ala Cys Ser Ala Ile Asp Ala Leu Val His Ala Thr Glu Ser Tyr Val
180 185 190
Ser Pro Lys Ser Asn Pro Tyr Thr Arg Leu Tyr Cys Arg Ala Ala Ile
195 200 205
Glu Ile Ile Ile Asp Val Phe Gln Gly Ile Ala Ala Asn Gly Pro Glu
210 215 220
Tyr Arg Phe Glu Arg Leu Gly Asp Met Leu Met Ala Ser Asn Tyr Ala
225 230 235 240
Gly Ile Ala Phe Gly Asn Thr Gly Val Gly Ala Val His Ala Leu Ser
245 250 255
Tyr Pro Leu Gly Gly Ser Tyr His Val Pro His Gly Glu Ser Asn Tyr
260 265 270
Gln Phe Phe Thr Glu Val Phe Lys Leu Tyr Asn Ala Lys Asn Pro Ser
275 280 285
Gly Asp Ile Ala Arg Leu Asn Asp Leu Leu Cys Val Gln Leu Gly Val
290 295 300
Asn Glu Asn Pro Tyr Pro Val Leu Asp Glu Leu Leu Gly Lys Leu Ile
305 310 315 320
Pro Lys Asn Lys Leu Arg Val Tyr Gly Met Lys Pro Glu Glu Ile Gln
325 330 335
Gly Phe Thr Ser Ser Val Leu Gln Thr Gln Gln Arg Leu Leu Ala Asn
340 345 350
Asn Tyr Val Ser Leu Ser Glu Glu Glu Ile Arg Glu Ile Tyr Lys Val
355 360 365
Leu Tyr
370
<210> 120
<211> 378
<212> PRT
<213> HBDHps
<400> 120
Met Ala Phe Ile Tyr Tyr Val Thr Gln Ile Gln Phe Glu Phe Gly Ala
1 5 10 15
Val Lys Leu Leu Lys Gln Glu Cys Glu Arg Val Gly Ile Thr Arg Pro
20 25 30
Leu Ile Val Thr Asp Pro Gly Val Lys Ala Ala Gly Ile Leu Gln Lys
35 40 45
Ala Leu Asp Ala Leu Pro Gly Ile Ala Val Thr Val Phe Asp Gln Thr
50 55 60
Pro Ser Asn Pro Thr Glu Ala Ala Val Arg Ala Ala Ala Ala Leu Tyr
65 70 75 80
Lys Ala Gly Gly Cys Asp Gly Leu Ile Ala Val Gly Gly Gly Ser Ala
85 90 95
Ile Asp Cys Ala Lys Gly Val Ala Ile Ala Ala Thr His Glu Gly Pro
100 105 110
Leu Thr Thr Tyr Ala Thr Ile Glu Gly Gly Ser Pro Lys Ile Ser Asp
115 120 125
Arg Val Ala Pro Leu Ile Ala Val Pro Thr Thr Ser Gly Thr Gly Ser
130 135 140
Glu Val Ala Arg Gly Ala Ile Ile Ile Val Asp Asp His Arg Lys Leu
145 150 155 160
Gly Phe His Ser Trp His Leu Val Pro Lys Thr Ala Ile Cys Asp Pro
165 170 175
Glu Leu Thr Leu Gly Leu Pro Ala Met Leu Thr Ala Ala Thr Gly Met
180 185 190
Asp Ala Ile Ala His Cys Met Glu Thr Phe Met Ser Pro Ala Phe Asn
195 200 205
Pro Pro Ala Asp Gly Ile Gly Leu Asp Gly Leu Glu Arg Gly Trp Ser
210 215 220
His Ile Glu Arg Ala Thr Arg Asp Gly Ser Asp Arg Glu Ala Arg Leu
225 230 235 240
Asn Leu Met Ser Ala Ser Met Gln Gly Ala Met Ala Phe Gln Lys Gly
245 250 255
Leu Gly Cys Val His Ser Leu Ser His Ser Leu Gly Gly Val Asp Pro
260 265 270
Arg Leu His His Gly Thr Leu Asn Ala Met Phe Leu Pro Ala Val Val
275 280 285
Arg Phe Asn Ala Ser Ala Glu Ser Val Gln Lys Glu Arg Arg Leu Asp
290 295 300
Arg Met Ala Arg Ala Met Gly Leu Arg Ser Gly Ser Asp Ile Pro Asp
305 310 315 320
Ala Ile Lys Asp Met Asn Ala Arg Leu Gly Leu Pro Thr Gly Leu Ala
325 330 335
Ala Met Gly Val Gln Arg Asp Trp Phe Asp Arg Ile Ile Val Gly Ala
340 345 350
Leu Ala Asp His Cys His Lys Thr Asn Pro Arg Leu Ala Ser Pro Glu
355 360 365
Glu Tyr Arg Glu Met Leu Glu Thr Ser Leu
370 375
<210> 121
<211> 288
<212> PRT
<213> HIBADHas
<400> 121
Met Gln Thr Ile Gly Phe Ile Gly Leu Gly Val Met Gly Lys Ser Met
1 5 10 15
Ala Arg His Leu Leu Arg Ala Gly Tyr Ser Leu Val Val Tyr Thr Arg
20 25 30
Thr Lys Gln Lys Ala Glu Asp Ile Leu Ala Glu Gly Ala Leu Trp Lys
35 40 45
Glu Thr Val Lys Asp Val Ala Lys Ala Ala Asp Val Val Met Thr Met
50 55 60
Val Gly Glu Pro Gln Asp Val Glu Tyr Val Tyr Phe Gly Asp Asp Gly
65 70 75 80
Ile Leu Ala His Ala Lys Arg Gly Thr Tyr Val Ile Asp Phe Thr Thr
85 90 95
Ser Lys Pro Ser Leu Ala Val Arg Ile His Glu Ala Ala Lys Glu Lys
100 105 110
Gly Ile Phe Val Leu Asp Ala Pro Val Ser Gly Gly Asp Ile Gly Ala
115 120 125
Arg Asp Gly Thr Leu Ser Ile Met Val Gly Gly Asp Lys Glu Ala Phe
130 135 140
Ser Ala Cys Met Pro Leu Leu Ser His Leu Gly Lys Asn Ile Val Trp
145 150 155 160
Gln Gly Glu Ala Gly Ala Gly Gln His Thr Lys Met Cys Asn Gln Ile
165 170 175
Ala Ile Ala Thr Asn Met Ile Gly Val Cys Glu Ala Leu Val Tyr Ala
180 185 190
Glu Lys Ala Gly Leu Asp Pro Glu Arg Val Leu Arg Ser Ile Ser Gln
195 200 205
Gly Ala Ala Ser Ser Trp Ser Leu Ser Asn Leu Ala Pro Arg Met Leu
210 215 220
Ala Gly Asp Phe Ala Pro Gly Phe Tyr Val Lys His Phe Ile Lys Asp
225 230 235 240
Met Gly Ile Ala Leu Ala Glu Ala Glu Thr Leu Gly Leu Ser Leu Pro
245 250 255
Gly Leu Lys Leu Ala Lys Gln Leu Tyr Glu Ser Leu Ala Glu Arg Gly
260 265 270
Glu Glu Gln Ser Gly Thr Gln Ala Leu Tyr Lys Trp Tyr Lys Glu Trp
275 280 285
<210> 122
<211> 296
<212> PRT
<213> HIBADHbc
<400> 122
Met Glu Ile Ala Phe Ile Gly Leu Gly Asn Met Gly Gly Pro Met Ala
1 5 10 15
Ala Asn Leu Leu Lys Ala Gly His Ala Leu Thr Val Phe Asp Leu Asp
20 25 30
Ala His Ala Val Asp Ala Ala Val Arg Ala Gly Ala Ala Ala Ala Thr
35 40 45
Ser Pro Arg Asp Ala Ala Ala Arg Gly Ser Leu Ile Ile Thr Met Leu
50 55 60
Pro Ala Ala Gln His Val Arg Ala Val Tyr Leu Gly Asp Asp Gly Val
65 70 75 80
Leu Ala Gly Ala Arg Ala Gly Ala Thr Leu Ile Asp Cys Ser Thr Ile
85 90 95
Asp Pro Gly Thr Val Arg Ala Val Ala Asp Ala Ala Ala Gln Arg Asp
100 105 110
Phe Pro Leu Ala Asp Ala Pro Val Ser Gly Gly Thr Gly Gly Ala Gln
115 120 125
Ala Gly Thr Leu Thr Phe Met Val Gly Ala Asp Gly Ala Leu Phe Glu
130 135 140
Arg Ile Arg Pro Val Leu Leu Asp Met Gly Arg Asn Val Val His Cys
145 150 155 160
Gly Gly Thr Gly Thr Gly Gln Ile Ala Lys Ile Cys Asn Asn Leu Leu
165 170 175
Leu Gly Ile Ser Met Met Gly Val Ser Glu Ala Met Ala Leu Gly Ala
180 185 190
Ala Leu Gly Ile Asp Pro Thr Val Leu Ala Gly Ile Ile Asn Thr Ser
195 200 205
Thr Gly Arg Cys Trp Ser Ser Asp Ala Tyr Asn Pro Tyr Pro Gly Val
210 215 220
Ser Asp Thr Ala Pro Ala Ala Arg Gly Tyr Ala Gly Gly Phe Ala Ala
225 230 235 240
Asn Leu Met Leu Lys Asp Leu Gly Leu Ala Thr Glu Ala Ala Arg Ser
245 250 255
Ala His Gln Pro Val Trp Met Gly Ala Leu Ala Gln Gln Leu Tyr Gln
260 265 270
Ser Met Ser Gln Gln Gly Leu Gly Thr Leu Asp Phe Ser Ala Cys Val
275 280 285
Lys Leu Tyr Glu Ala Gln Pro Ala
290 295
<210> 123
<211> 295
<212> PRT
<213> HIBADHma
<400> 123
Met Ala Lys Ile Thr Phe Ile Gly Leu Gly Asn Met Gly Gly Pro Met
1 5 10 15
Ala Gly Asn Leu Val Lys Ala Gly His Glu Val Thr Val Phe Asp Leu
20 25 30
Ser Lys Asp Ala Val Ala Ala Leu Val Ser Glu Gly Ala Lys Thr Ala
35 40 45
Gly Thr Ala His Glu Ala Ala Ser Gly Ala Glu Cys Val Ile Thr Met
50 55 60
Leu Pro Ala Gly Gln His Val Glu Ala Val Tyr Leu Gly Asp Asp Gly
65 70 75 80
Leu Leu Ala Lys Leu Pro Ala Gly Thr Leu Val Ile Asp Ser Ser Thr
85 90 95
Ile Ala Pro Glu Thr Ala Arg Gly Val Ala Glu Val Ala Arg Ala Lys
100 105 110
Asp Ile Pro Phe Leu Asp Ala Pro Val Ser Gly Gly Val Gly Gly Ala
115 120 125
Lys Ala Gly Thr Leu Thr Phe Ile Cys Gly Gly Ser Glu Glu Ala Phe
130 135 140
Asn Lys Ala Lys Pro Ile Leu Asp Thr Met Gly Lys Asn Ile Phe His
145 150 155 160
Ala Gly Asp His Gly Ser Gly Gln Val Ala Lys Ile Cys Asn Asn Met
165 170 175
Leu Leu Ala Ile Leu Met Ser Gly Thr Ser Glu Ala Leu Ala Leu Gly
180 185 190
Val Lys Asn Gly Leu Asp Pro Ala Val Leu Ser Glu Ile Met Lys Gln
195 200 205
Ser Ser Gly Gly Asn Trp Ala Leu Asn Val Tyr Asn Pro Trp Pro Gly
210 215 220
Val Met Glu Gly Val Pro Ala Ser Arg Asn Tyr Glu Gly Gly Phe Leu
225 230 235 240
Val Asn Leu Met Thr Lys Asp Leu Gly Leu Ala Phe Asp Asn Ala Val
245 250 255
Lys Asn Gln Ala Ser Ile Pro Met Gly Ser Leu Ala Arg Asn Leu Phe
260 265 270
Gln Leu His Ala Gly Gln Gly Asn Gly Ser Leu Asp Phe Ser Ser Ile
275 280 285
Gln Arg Leu Tyr Lys Pro Glu
290 295
<210> 124
<211> 298
<212> PRT
<213> HIBADHpa
<400> 124
Met Thr Asp Ile Ala Phe Leu Gly Leu Gly Asn Met Gly Gly Pro Met
1 5 10 15
Ala Ala Asn Leu Leu Lys Ala Gly His Arg Val Asn Val Phe Asp Leu
20 25 30
Gln Pro Lys Ala Val Leu Gly Leu Val Glu Gln Gly Ala Gln Gly Ala
35 40 45
Asp Ser Ala Leu Gln Cys Cys Glu Gly Ala Glu Val Val Ile Ser Met
50 55 60
Leu Pro Ala Gly Gln His Val Glu Ser Leu Tyr Leu Gly Asp Asp Gly
65 70 75 80
Leu Leu Ala Arg Val Ala Gly Lys Pro Leu Leu Ile Asp Cys Ser Thr
85 90 95
Ile Ala Pro Glu Thr Ala Arg Lys Val Ala Glu Ala Ala Ala Ala Lys
100 105 110
Gly Leu Thr Leu Leu Asp Ala Pro Val Ser Gly Gly Val Gly Gly Ala
115 120 125
Arg Ala Gly Thr Leu Ser Phe Ile Val Gly Gly Pro Ala Glu Gly Phe
130 135 140
Ala Arg Ala Arg Pro Val Leu Glu Asn Met Gly Arg Asn Ile Phe His
145 150 155 160
Ala Gly Asp His Gly Ala Gly Gln Val Ala Lys Ile Cys Asn Asn Met
165 170 175
Leu Leu Gly Ile Leu Met Ala Gly Thr Ala Glu Ala Leu Ala Leu Gly
180 185 190
Val Lys Asn Gly Leu Asp Pro Ala Val Leu Ser Glu Val Met Lys Gln
195 200 205
Ser Ser Gly Gly Asn Trp Ala Leu Asn Leu Tyr Asn Pro Trp Pro Gly
210 215 220
Val Met Pro Gln Ala Pro Ala Ser Asn Gly Tyr Ala Gly Gly Phe Gln
225 230 235 240
Val Arg Leu Met Asn Lys Asp Leu Gly Leu Ala Leu Ala Asn Ala Gln
245 250 255
Ala Val Gln Ala Ser Thr Pro Leu Gly Ala Leu Ala Arg Asn Leu Phe
260 265 270
Ser Leu His Ala Gln Ala Asp Ala Glu His Glu Gly Leu Asp Phe Ser
275 280 285
Ser Ile Gln Lys Leu Tyr Arg Gly Lys Asp
290 295
<210> 125
<211> 295
<212> PRT
<213> HIBADHxc
<400> 125
Met Ser Lys Ile Ala Phe Ile Gly Leu Gly Asn Met Gly Gly Pro Met
1 5 10 15
Val Ala Asn Leu Ile Lys Ala Gly His Glu Leu Arg Val Phe Asp Leu
20 25 30
Val His Ser Ala Leu Asp Thr Ala His Ala Ala Gly Ala Gln Val Ala
35 40 45
Ser Ser Ala Ser Asp Thr Leu His Gly Ala Glu Val Val Ile Ser Met
50 55 60
Leu Pro Ala Ser Lys His Val Glu Gly Leu Tyr Leu Gly Asp Ala Gly
65 70 75 80
Ile Leu Ala His Ile Pro Ala Gly Ala Leu Val Ile Asp Cys Ser Thr
85 90 95
Ile Ala Pro Ala Met Ala Arg Thr Val Ala Thr Ala Ala His Ala Arg
100 105 110
Gly Leu Asp Met Leu Asp Ala Pro Val Ser Gly Gly Thr Ala Gly Ala
115 120 125
Ala Ala Gly Thr Leu Thr Phe Ile Val Gly Gly Ala Ala Gln Ala Leu
130 135 140
Glu Arg Ala Arg Pro Val Leu Gln Ala Met Gly Lys Asn Leu Phe His
145 150 155 160
Val Gly Asp Asn Gly Ala Gly Gln Val Ala Lys Leu Cys Asn Asn Met
165 170 175
Ala Leu Gly Val Ile Met Ala Ala Thr Gly Glu Ala Ile Ala Leu Gly
180 185 190
Val Ala Gln Gly Leu Asp Pro Ala Val Leu Ser Gln Met Met Ala Val
195 200 205
Ser Thr Gly Arg Ser Trp Ala Thr Glu Val Cys Asn Pro Trp Pro Gly
210 215 220
Val Leu Glu Asn Ala Pro Ala Ser Arg Gly Tyr Ser Gly Gly Phe Gly
225 230 235 240
Asn Asp Leu Met Leu Lys Asp Leu Gly Leu Val Ala Glu Ala Ala Val
245 250 255
Gln Ala Gly Ala Ser Ile Pro Leu Gly Glu Leu Ala Arg Asn Leu Tyr
260 265 270
Ala Met Asn Ser Arg Ala Gly Asn Gly Ala Leu Asp Phe Ser Ser Val
275 280 285
Val Lys Leu Val Ala Lys Val
290 295
<210> 126
<211> 253
<212> PRT
<213> HPDHam
<400> 126
Met Thr Gln Gln Thr Val Phe Val Thr Gly Ala Ser Ala Gly Phe Gly
1 5 10 15
Ala Ala Ile Ala Arg Arg Phe Val Ala Glu Gly Ala Arg Val Ile Ala
20 25 30
Val Ala Arg Ser Glu Asp Lys Leu Glu Lys Leu Ala Gly Glu Leu Gly
35 40 45
Asp Ala Val Leu Pro Val Lys Leu Asp Val Ser Asp Pro Glu Ala Val
50 55 60
Lys Glu Val Val Thr Asn Leu Pro Ala Asp Trp Gln Gln Val Asp Val
65 70 75 80
Leu Val Asn Asn Ala Gly Leu Ala Lys Gly Leu Glu Pro Ala His Lys
85 90 95
Ala Asp Leu Gln Asp Trp Asp Glu Met Ile Ala Thr Asn Val Arg Gly
100 105 110
Leu Thr His Val Thr Arg Ala Leu Leu Pro Gly Met Val Glu Arg Gly
115 120 125
Arg Gly His Val Ile Asn Ile Gly Ser Ile Ala Gly Thr Tyr Pro Tyr
130 135 140
Pro Gly Gly Asn Val Tyr Gly Ala Thr Lys Ala Phe Val His Gln Phe
145 150 155 160
Ser Leu Asn Leu Arg Ser Asp Leu His Gly Thr Gly Val Arg Val Thr
165 170 175
Asn Val Glu Pro Gly Met Val Gly Gly Thr Asp Phe Ser Lys Val Arg
180 185 190
Phe Asp Gly Asp Gln Ala Lys Ala Asp Lys Val Tyr Glu Gly Thr Thr
195 200 205
Pro Leu Thr Ala Glu Asp Val Ala Glu Ser Val Phe Trp Ala Ala Asn
210 215 220
Gln Pro Ala His Val Asn Ile Asn Val Ile Glu Leu Met Pro Val Val
225 230 235 240
Gln Ser Phe Ser Pro Leu Gln Ile Phe Arg Asp Asn Ser
245 250
<210> 127
<211> 550
<212> PRT
<213> HPDHbs
<400> 127
Met Thr Ala Gly Lys Gly Lys Gly Ala Lys Val Asp Pro Ala Gly Gly
1 5 10 15
Lys Ala Ala Thr Gly Arg Leu Ala Gly Lys Val Ala Leu Val Thr Gly
20 25 30
Ala Ala Gly Asn Leu Gly Gly Ala Ile Ile Arg Arg Phe Ile Ala Glu
35 40 45
Gly Ala Thr Leu Val Met Thr Gly Arg Thr Lys Glu Arg Leu Ala Ala
50 55 60
Ala Lys Gln Ala Leu Leu Asp Glu Thr Gly Val Glu Glu Ala Arg Val
65 70 75 80
Phe Thr Val Ile Leu Asp Gly Ala Asp Pro Asp Ser Val Arg Ala Gly
85 90 95
Ile Asp Gln Val Arg Ala Ala Leu Gly Arg Ile Asp Val Leu Val Asn
100 105 110
Asn Ala Gly Ser Ala Gly Pro Arg Gln Thr Leu Glu Asn Val Pro Leu
115 120 125
Thr Arg Ala Glu Met Glu Ala Asn Gly Asp Ser Glu Thr Val Ala Asp
130 135 140
Ala Met Arg Asn Leu Leu Gly Val Ser Trp Asn Leu Thr Arg Ala Ala
145 150 155 160
Ala Pro Ile Met Pro Val Gly Ala Ser Val Val Asn Ile Ser Thr Ile
165 170 175
Phe Ser His Thr Arg Tyr Tyr Gly Arg Thr Ala Tyr Val Val Pro Lys
180 185 190
Ala Ala Leu Asn Ala Leu Ser Gln Glu Leu Ala Lys Glu Leu Gly Ala
195 200 205
Ser Gly Ile Arg Val Asn Thr Val Phe Pro Gly Pro Ile Glu Ser Glu
210 215 220
Arg Ile Arg Thr Val Phe Ala Ala Met Asp Ala Leu Gln Gly Glu Pro
225 230 235 240
Glu Asn Ser Thr Ala Asn His Phe Met Gly Arg Met Thr Leu Glu Arg
245 250 255
Ala Ile Gly Asp Ala Pro Lys Arg Lys Thr Leu Pro Val Pro Gly Asp
260 265 270
Ile Ala Asp Ala Cys Leu Phe Leu Ala Ser Asp Glu Ser Ala Ala Leu
275 280 285
Asn Gly Asn Glu Ile Asp Val Thr His Gly Met Gln Val Arg Lys Asp
290 295 300
Ser Arg Ser Thr Tyr Met Thr Arg Pro Ser Met Arg Ser Leu Asp Gly
305 310 315 320
Ala Gly Leu Thr Val Leu Ile Ala Ala Gly Glu Ala Trp Glu Asp Ala
325 330 335
Leu Asp Met Ala Lys Ile Gln Ala Ala Cys Gly Ala Arg Val Leu Leu
340 345 350
Gly Met Thr Arg Gln Val Asp Val Ala Gln Ala Glu Ala Arg Val Lys
355 360 365
Ala Gln Gly Ala Glu Glu Gly Leu Thr Ile Met Arg Phe Asn Arg Ala
370 375 380
Glu Pro Asp Gly Met Glu Ala Ala Ile Arg Ala Tyr Ser Asp Ala His
385 390 395 400
Gly Ala Ile His Ser Ala Ile Val Leu Pro Val Lys Pro Ala Gly His
405 410 415
Phe Gly Asp Ser Leu Val Glu Ala Ser Asp Ala Glu Val Asp Ala Leu
420 425 430
Ile Asp Ile Glu Leu Thr Gly Ala Ile Ala Val Ala Arg Thr Leu Arg
435 440 445
Arg Tyr Trp Lys Thr Cys Ser Asp Leu Met Gln Asp Pro Arg Phe Val
450 455 460
Phe Met Ser Gly Pro Ser Asp Gly Lys Gly Asn Lys Ala Gly Arg Ile
465 470 475 480
Leu Ala Ala Ala Ile Ala Gln Leu Ile Thr Ile Trp Arg Asp Glu Ser
485 490 495
Arg Val Asp Phe Ala Gln Gly Arg Gln Lys Arg Ala Ala Trp Gly Asn
500 505 510
Gln Ile Val Arg Tyr Thr Asn Glu Glu Asp Glu Asn Thr Arg Phe Ala
515 520 525
Ala Gly His Ala Thr Arg Ile Val Phe Lys Glu Gln Lys Ile Thr Glu
530 535 540
Thr Ser Leu Tyr Leu Pro
545 550
<210> 128
<211> 550
<212> PRT
<213> HPDHca
<400> 128
Met Ser Gly Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly
1 5 10 15
Gly Ala Gly Asn Ile Gly Ser Glu Leu Thr Arg Arg Phe Leu Ala Glu
20 25 30
Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Arg Ala Lys Leu Thr Ala
35 40 45
Leu Ala Glu Arg Met Gln Ala Glu Ala Gly Val Pro Ala Lys Arg Ile
50 55 60
Asp Leu Glu Val Met Asp Gly Ser Asp Pro Val Ala Val Arg Ala Gly
65 70 75 80
Ile Glu Ala Ile Val Ala Arg His Gly Gln Ile Asp Ile Leu Val Asn
85 90 95
Asn Ala Gly Ser Ala Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu
100 105 110
Thr Glu Ala Glu Leu Gly Pro Gly Ala Glu Glu Thr Leu His Ala Ser
115 120 125
Ile Ala Asn Leu Leu Gly Met Gly Trp His Leu Met Arg Ile Ala Ala
130 135 140
Pro His Met Pro Val Gly Ser Ala Val Ile Asn Val Ser Thr Ile Phe
145 150 155 160
Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Thr Pro Lys Ala
165 170 175
Ala Leu Asn Ala Leu Ser Gln Leu Ala Ala Arg Glu Leu Gly Ala Arg
180 185 190
Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Asp Arg
195 200 205
Ile Arg Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu
210 215 220
Gly Asp Thr Ala His His Phe Leu Asn Thr Met Arg Leu Cys Arg Ala
225 230 235 240
Asn Asp Gln Gly Ala Leu Glu Arg Arg Phe Pro Ser Val Gly Asp Val
245 250 255
Ala Asp Ala Ala Val Phe Leu Ala Ser Ala Glu Ser Ala Ala Leu Ser
260 265 270
Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Ala Cys Ser
275 280 285
Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Ser
290 295 300
Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met
305 310 315 320
Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly
325 330 335
Phe Arg Ser Ala Ala Ala Leu Ala Gln Phe Glu Gln Ala Val Asn Glu
340 345 350
Ser Arg Arg Leu Ala Gly Ala Asp Phe Thr Pro Pro Ile Ala Leu Pro
355 360 365
Leu Asp Pro Arg Asp Pro Ala Thr Ile Asp Ala Val Phe Asp Trp Gly
370 375 380
Ala Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala
385 390 395 400
Thr Ser His Glu Pro Ala Pro Cys Val Ile Glu Val Asp Asp Glu Arg
405 410 415
Val Leu Asn Phe Leu Ala Asp Glu Ile Thr Gly Thr Ile Val Ile Ala
420 425 430
Ser Arg Leu Ala Arg Tyr Trp Gln Ser Gln Arg Leu Thr Pro Gly Ala
435 440 445
Arg Ala Arg Gly Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Asp Gln
450 455 460
Asn Gly Asn Val Tyr Gly Arg Ile Gln Ser Ala Ala Ile Gly Gln Leu
465 470 475 480
Ile Arg Val Trp Arg His Glu Ala Glu Leu Asp Tyr Gln Arg Ala Ser
485 490 495
Ala Ala Gly Asp His Val Leu Pro Pro Val Trp Ala Asn Gln Ile Val
500 505 510
Arg Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp
515 520 525
Thr Ala Gln Leu Leu His Ser Gln Arg His Ile Asn Glu Ile Thr Leu
530 535 540
Asn Ile Pro Ala Asn Ile
545 550
<210> 129
<211> 550
<212> PRT
<213> HPDHcag
<400> 129
Met Ser Val Thr Gly Arg Leu Ala Gly Lys Ile Ala Leu Ile Thr Gly
1 5 10 15
Gly Ala Gly Asn Ile Gly Ser Glu Met Thr Arg Arg Phe Leu Ala Glu
20 25 30
Gly Ala Thr Val Ile Ile Ser Gly Arg Asn Ser Ala Lys Leu Ala Ala
35 40 45
Leu Ala Glu Arg Leu Arg Ser Glu Ala Gly Val Pro Ala Lys Arg Ile
50 55 60
Asp Leu Glu Val Met Asp Gly Ser Asp Pro Ala Ala Val Arg Ala Gly
65 70 75 80
Val Ala Ala Ile Ile Gly Arg His Gly His Ile Asp Ile Leu Val Asn
85 90 95
Asn Ala Gly Ser Thr Gly Ala Gln Arg Arg Leu Ala Glu Ile Pro Leu
100 105 110
Asn Glu Thr Asp Arg Asp Leu Asp Asp Glu Glu Ala Leu Ser Thr Ser
115 120 125
Val Ala Asn Leu Leu Gly Met Ala Trp His Leu Met Arg Ile Leu Ser
130 135 140
Pro His Met Pro Pro Gly Ser Ala Ile Ile Asn Ile Ser Thr Ile Phe
145 150 155 160
Ser Arg Ala Glu Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ala
165 170 175
Ala Leu Asn Thr Leu Thr Gln Ile Ala Ala Arg Glu Leu Gly Ile Arg
180 185 190
Gly Ile Arg Val Asn Thr Ile Phe Pro Gly Pro Ile Glu Ser Glu Arg
195 200 205
Ile Gln Thr Val Phe Gln Arg Met Asp Gln Leu Lys Gly Arg Pro Glu
210 215 220
Gly Asp Thr Ala Ser Gln Phe Leu Ala Thr Met Arg Leu Tyr Arg Ala
225 230 235 240
Asn Asp Gln Gly Gln Leu Glu Arg Arg Phe Pro Thr Ile Cys Asp Val
245 250 255
Ala Asp Ala Ala Val Phe Leu Ala Ser Asp Glu Ala Ala Ala Leu Thr
260 265 270
Gly Glu Thr Ile Glu Val Thr His Gly Met Glu Leu Pro Thr Ser Ser
275 280 285
Glu Thr Ser Leu Leu Ala Arg Thr Asp Leu Arg Thr Ile Asp Ala Asn
290 295 300
Gly Arg Thr Thr Leu Ile Cys Ala Gly Asp Gln Ile Glu Glu Val Met
305 310 315 320
Ala Leu Thr Gly Met Leu Arg Thr Cys Gly Ser Glu Val Ile Ile Gly
325 330 335
Phe Arg Ser Glu Ala Ala Leu Ala Gln Phe Glu Gln Ala Ile Gly Glu
340 345 350
Ser Arg Arg Leu Ala Gly Glu Ser Phe Ile Pro Pro Ile Ala Leu Pro
355 360 365
Ile Asp Leu Arg Asn Pro Ser Thr Ile Asp Ala Leu Phe Asp Trp Ala
370 375 380
Gly Glu Asn Thr Gly Gly Ile His Ala Ala Val Ile Leu Pro Ala Ser
385 390 395 400
Gly Arg Glu Pro Ala Thr Gln Val Ile Asp Ile Asp Asp Ala His Val
405 410 415
Gln Ala Phe Leu Asn Asp Glu Ile Val Gly Ser Ile Ile Ile Ala Ser
420 425 430
Arg Leu Ala Arg Tyr Trp Gln Ala Gln Arg Ile Ala Pro Gly Ala Arg
435 440 445
Ala Arg Glu Pro Arg Val Ile Phe Leu Ser Asn Gly Ala Ser Thr Ala
450 455 460
Gly Asn Pro Tyr Gly Arg Ile Gln Ser Ala Ala Ile Glu Gln Leu Ile
465 470 475 480
Arg Val Trp Arg His Glu Ala Ala Leu Asp Tyr Glu Arg Ala Thr Ala
485 490 495
Ala Gly Glu Arg Val Leu Pro Ala Val Trp Ala Ser Gln Ile Val Arg
500 505 510
Phe Ala Asn Arg Ser Leu Glu Gly Leu Glu Phe Ala Cys Ala Trp Thr
515 520 525
Ala Gln Leu Leu His Ser Gln Arg Arg Ile Asn Glu Ile Thr Leu Thr
530 535 540
Ile Pro Ala Asp Ile Ser
545 550
<210> 130
<211> 550
<212> PRT
<213> HPDHct
<400> 130
Met Arg Arg Thr Lys Pro Glu Cys Leu Arg Arg Asn Glu Ser Thr Met
1 5 10 15
Arg Leu Asn Gly Lys Val Ala Leu Ile Thr Gly Ala Ala Gly Gly Ile
20 25 30
Gly Ser Val Val Thr Arg Met Tyr Leu Arg Glu Gly Ala Arg Val Ala
35 40 45
Ile Ser Ser Arg Ser Leu Glu Arg Ala Glu Gln Phe Arg Ala Ala Leu
50 55 60
Met Ala Glu Gly Phe Ser Gly Asp Asp Ile Leu Pro Val Ala Phe Ala
65 70 75 80
Ser Thr Asp Leu Ala Gly Met Gln Thr Ala Ile Glu Ala Ile Ala Ala
85 90 95
Arg Trp Gly Gly Leu Asp Val Leu Ile Asn Asn Ala Gly Ser Ala Gly
100 105 110
Ala Lys Gln Pro Leu Phe Arg Ile Pro Phe Thr Ser Glu Asp Leu Ala
115 120 125
Gln Leu Ala Ala Glu Gly Phe Thr Glu Thr Glu Thr Met Arg Glu Ser
130 135 140
Ala Ala Asn Leu Leu Gly Met Pro Trp His Leu Thr Arg Leu Ala Leu
145 150 155 160
Pro Tyr Leu Arg Val Gly Ala Ser Ile Ile Asn Val Ser Thr Ile Phe
165 170 175
Ser Arg Thr Asn Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ser
180 185 190
Ala Leu Asn Ala Leu Ser Leu Gly Leu Ala Lys Gln Leu Gly Thr Thr
195 200 205
Asp Arg Ala Val Arg Val Asn Thr Val Phe Pro Gly Pro Ile Asp Ser
210 215 220
Asp Arg Ile Arg Thr Val Phe Gly Ala Met Asp Arg Leu Lys Gly Val
225 230 235 240
Pro Glu Gly Thr Thr Ala Gln Glu Phe Phe Asp Ile Met Ile Leu Ala
245 250 255
Arg Ala Arg Gly Gly Glu Ala Pro Thr Lys Cys Tyr Pro Thr Gln Ala
260 265 270
Asp Val Ala His Val Ile Leu Phe Leu Gly Ser Asp Glu Ser Ala Ala
275 280 285
Ile Ser Gly His Asn Phe Glu Ile Thr His Gly Met Gln Val Lys Ala
290 295 300
Gln Ser Arg Thr Lys Leu Thr Ala Trp Pro Asp Gln Arg Leu Ile Asp
305 310 315 320
Leu Ser Arg Arg Val Val Leu Ile Val Gly Gly Glu Gln Val His Asp
325 330 335
Ala Val Thr Ile Ala Ala Ser Gln Gln Ser Tyr Gly Ala Ser Val Ile
340 345 350
Leu Thr Phe Arg Gln Pro Glu Ala Val Asn Thr Ala Lys Ala Gln Leu
355 360 365
Glu Ser Arg Gly Leu Asn His Ser Ile Ala Val Thr Trp Leu Asp Pro
370 375 380
Leu Arg Arg Glu Ser Val Thr Ser Val Phe Asp Leu Ile Arg Glu Arg
385 390 395 400
Tyr His Arg Leu Asp Thr Val Ile Val Phe Pro Ala His Ala Ala Gly
405 410 415
His Tyr Gly Pro Glu Leu Ile Pro Asp Glu Pro Glu Arg Ile Arg Gln
420 425 430
Phe Thr Asp Glu Ala Ile Leu Gly Thr Thr Ala Phe Ala Ala Gln Leu
435 440 445
Ser Arg Phe Leu Leu Ala Phe Asp Ala Glu Asn Glu Leu Gln Glu Pro
450 455 460
Val Asn Val Leu Phe Val Thr Asn Glu His Asp Gly Ala Thr Asn Ala
465 470 475 480
Phe Glu Asp Ile Tyr Arg Ala Ala Val Glu Gln Leu Leu Arg Val Trp
485 490 495
Arg His Glu Asp Glu Leu Gln Val Ala Ala Gly Thr Arg Arg Phe Ala
500 505 510
Val Arg Thr Asn Gln Ile Val Arg Tyr Ala Asn Ala Glu Pro Asp Asn
515 520 525
Leu Lys Phe Thr Ala Asp Trp Leu Ala Thr Leu Ala Asn Arg Val Arg
530 535 540
Ile Phe Asp Glu Leu Asn
545 550
<210> 131
<211> 248
<212> PRT
<213> HPDHec
<400> 131
Met Ile Val Leu Val Thr Gly Ala Thr Ala Gly Phe Gly Glu Cys Ile
1 5 10 15
Thr Arg Arg Phe Ile Gln Gln Gly His Lys Val Ile Ala Thr Gly Arg
20 25 30
Arg Gln Glu Arg Leu Gln Glu Leu Lys Asp Glu Leu Gly Asp Asn Leu
35 40 45
Tyr Ile Ala Gln Leu Asp Val Arg Asn Arg Ala Ala Ile Glu Glu Met
50 55 60
Leu Ala Ser Leu Pro Ala Glu Trp Cys Asn Ile Asp Ile Leu Val Asn
65 70 75 80
Asn Ala Gly Leu Ala Leu Gly Met Glu Pro Ala His Lys Ala Ser Val
85 90 95
Glu Asp Trp Glu Thr Met Ile Asp Thr Asn Asn Lys Gly Leu Val Tyr
100 105 110
Met Thr Arg Ala Val Leu Pro Gly Met Val Glu Arg Asn His Gly His
115 120 125
Ile Ile Asn Ile Gly Ser Thr Ala Gly Ser Trp Pro Tyr Ala Gly Gly
130 135 140
Asn Val Tyr Gly Ala Thr Lys Ala Phe Val Arg Gln Phe Ser Leu Asn
145 150 155 160
Leu Arg Thr Asp Leu His Gly Thr Ala Val Arg Val Thr Asp Ile Glu
165 170 175
Pro Gly Leu Val Gly Gly Thr Glu Phe Ser Asn Val Arg Phe Lys Gly
180 185 190
Asp Asp Gly Lys Ala Glu Lys Thr Tyr Gln Asn Thr Val Ala Leu Thr
195 200 205
Pro Glu Asp Val Ser Glu Ala Val Trp Trp Val Ser Thr Leu Pro Ala
210 215 220
His Val Asn Ile Asn Thr Leu Glu Met Met Pro Val Thr Gln Ser Tyr
225 230 235 240
Ala Gly Leu Asn Val His Arg Gln
245
<210> 132
<211> 196
<212> PRT
<213> HPDHed
<400> 132
Met Ser Glu Ala Ile Thr Pro Gly Ala Leu Glu Thr Leu Phe Thr Gly
1 5 10 15
Ala Arg Thr His Asn Gly Trp Gln Asn Met Pro Val Ser Asp Glu Thr
20 25 30
Leu Arg Glu Ile Tyr Asn Leu Met Lys Trp Gly Pro Thr Ser Ala Asn
35 40 45
Cys Ser Pro Ala Arg Ile Val Phe Val Arg Ser Ala Glu Gly Lys Glu
50 55 60
Lys Leu Arg Pro Ala Leu Ser Ser Gly Asn Leu Glu Lys Thr Leu Thr
65 70 75 80
Ala Pro Val Thr Ala Ile Val Ala Trp Asp Gly Glu Phe Tyr Glu Arg
85 90 95
Leu Pro Glu Leu Phe Pro His Gly Asp Ala Lys Ser Trp Phe Thr Ala
100 105 110
Ser Pro Ala Leu Ala Glu Glu Thr Ala Phe Arg Asn Ser Ser Leu Gln
115 120 125
Ala Ala Tyr Leu Ile Phe Ala Cys Arg Ala Leu Gly Leu Asp Thr Gly
130 135 140
Pro Met Ser Gly Phe Asp Arg Gln Lys Val Asp Glu Ala Phe Phe Thr
145 150 155 160
Gly Thr Thr Leu Lys Ser Asn Leu Leu Ile Asn Ile Gly Tyr Gly Asp
165 170 175
Pro Gly Lys Leu Tyr Gly Arg Leu Pro Arg Leu Asp Phe Asp Asp Ala
180 185 190
Cys Gly Leu Ala
195
<210> 133
<211> 550
<212> PRT
<213> HPDHgb
<400> 133
Met Asn Asp Met Ser Asp Ile Pro Gln Asp Leu Leu Thr Gln Ser Ile
1 5 10 15
Ala Gly Arg Arg Leu Asp Asn Lys Cys Ile Ile Leu Thr Gly Ala Ala
20 25 30
Gly Ser Ile Gly Ser Phe Ile Thr Arg Gln Leu Leu Arg Glu Gly Ala
35 40 45
Arg Val Met Leu Thr Gly Arg Asp Gln Ser Lys Leu Asp Asp Phe Ile
50 55 60
Gly Asp Leu Val Ser Glu Gly Phe Glu Glu Thr Phe Met Val Ser Ala
65 70 75 80
Thr Gly Asp Cys Ala Asp Pro Glu Val Cys Arg Glu Ile Val Ala Lys
85 90 95
Thr Val Ala Ala Phe Gly Pro Ile Asp Val Leu Val Asn Asn Ala Gly
100 105 110
Ala Ala Gly Pro Lys Phe Thr Leu Arg Asp Ile Pro Phe Thr Glu Ala
115 120 125
Glu Gln Arg Ala Ala Asp Ser Asp Gln Thr Met Phe Asp Ser Ala Met
130 135 140
Asn Leu Leu Gly Ala Pro Trp Asn Met Ala Arg Ala Ala Ala Pro His
145 150 155 160
Met Ser Ala Gly Ala Ser Ile Ile Asn Val Ser Thr Ile Phe Ser Arg
165 170 175
Thr His Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ser Gly Leu
180 185 190
Asn Ala Leu Gly Lys Gly Leu Ala Leu Glu Leu Gly Glu Lys Gln Gly
195 200 205
Ile Arg Val Asn Thr Leu Phe Pro Gly Pro Ile Glu Ser Glu Arg Ile
210 215 220
Glu Ser Val Phe Ala Arg Met Asp Glu Leu Gln Gly Val Glu Pro Gly
225 230 235 240
Ser Thr Gly Lys Glu Phe Arg Asp Leu Met Ile Thr Thr Arg Thr Ser
245 250 255
Asp Gly Gly Leu Glu Tyr Lys Tyr Pro Thr Pro Thr Asp Val Ala Ser
260 265 270
Gly Ile Val Trp Leu Ala Ser Asp Glu Ser Ser Ala Leu Ser Gly His
275 280 285
His Ile Glu Val Thr Asn Gly Met Gln Val Pro Ala Gln Ser Arg Ser
290 295 300
Gln Leu Val Ser Trp Pro Asp Lys Arg Leu Glu Asp Leu Ser Gly Gln
305 310 315 320
Val Val Leu Ile Leu Gly Gly Asp Asp Tyr Gln Glu Ala Val Thr Phe
325 330 335
Ala Glu Arg Gln Ile Gln Ser Gly Ala Arg Val Met Leu Ala Phe Arg
340 345 350
Asn Leu Asn Thr Val Gly His Ala Arg Ser Leu Val Gln Ser Arg Asn
355 360 365
Leu Gly Asp Val Gln Leu Ser His Leu Asp Pro Leu Arg Arg Glu Ser
370 375 380
Val Asp Arg Ser Met Gln Leu Ile Ala Asp His Phe Gly Arg Leu Asp
385 390 395 400
Gly Val Ile Leu Leu Pro Gln Lys Pro Asn Gly Glu Tyr Gly Tyr Ser
405 410 415
Leu Thr Thr Ala Asn Asp Glu Asp Val Ala Asn Phe Val Arg Asp Glu
420 425 430
Val Val Ala Pro Val Ala Phe Ala Ser Ala Phe Ala Arg Asn Met Asp
435 440 445
Gln Leu Phe Ala Glu Gly Glu Pro Pro Ala Ile Val Tyr Val Thr Asn
450 455 460
Pro Ser Asp Arg His Gly Asn Leu Met Asn Glu Ile Ile Arg Ala Ser
465 470 475 480
Val Glu Ala Leu Ile Arg Gly Trp Arg His Glu Asp Glu Thr Leu Ala
485 490 495
Asn Thr Gly Glu Leu Pro Trp Ala Val Gln Pro Asn Gln Met Val Arg
500 505 510
Tyr Asp Thr Glu Asp Arg Glu Ala Leu Thr Phe Ala Ala Asp Trp Ala
515 520 525
Ala Thr Leu Thr Asn Arg Val Arg Lys Met Asp Ser Ile Asn Leu Trp
530 535 540
Leu Pro Arg Ser Ile Lys
545 550
<210> 134
<211> 252
<212> PRT
<213> HPDHhw
<400> 134
Met Ile Val Leu Val Thr Gly Ala Ser Ser Gly Phe Gly Arg Ala Ile
1 5 10 15
Ala Lys Lys Phe Gly Glu Asn Gly His Lys Val Ile Ala Leu Ala Arg
20 25 30
Arg Lys Glu Arg Leu Gln Ser Leu Gln Lys Glu Ile Pro Tyr Cys Glu
35 40 45
Ile Leu Pro Cys Asp Ile Cys Asp Lys Lys Ala Val Lys Gln Ala Leu
50 55 60
Glu Ala Leu Pro Gln Asp Phe Lys Ser Ile Asp Ile Leu Val Asn Asn
65 70 75 80
Ala Gly Leu Ala Leu Gly Ile Glu Pro Ala Gln Met Cys Asp Phe Lys
85 90 95
Asp Trp Glu Arg Met Ile Ser Val Asn Val Thr Ala Leu Ser Tyr Leu
100 105 110
Thr His Leu Val Leu Pro Gln Met Val Glu Arg Lys Ser Gly His Ile
115 120 125
Ile Thr Leu Gly Ser Ile Ala Gly Lys Tyr Pro Tyr Pro Gly Gly Asn
130 135 140
Val Tyr Gly Ala Thr Lys Ala Phe Val Lys Gln Phe Ala Leu Gly Leu
145 150 155 160
Arg Ala Asp Leu Ala Gly Thr Asn Val Arg Val Ser Asp Ile Gln Pro
165 170 175
Gly Leu Ala Glu Ser Glu Phe Ser Leu Val Arg Phe Lys Gly Asp Lys
180 185 190
Lys Arg Ala Asn Ala Leu Tyr Glu Gly Ser Asn Ala Leu Gln Pro Gln
195 200 205
Asp Ile Ala Glu Ala Val Tyr Trp Val Ala Thr Leu Pro Lys His Ile
210 215 220
Asn Ile Asn Thr Leu Glu Met Met Pro Thr Ser Gln Ser Phe Ser Thr
225 230 235 240
Leu Ala Val His Lys Glu Tyr Ser Phe Leu Thr Lys
245 250
<210> 135
<211> 267
<212> PRT
<213> HPDHka
<400> 135
Met Ser Gln Gly Arg Arg Ala Ala Asp Arg Leu Ala Gly Lys Val Val
1 5 10 15
Leu Ile Thr Gly Ala Ser Ala Gly Ile Gly Glu Ala Thr Ala Trp Glu
20 25 30
Tyr Leu Phe Ala Ser Asn Gly Gln Ala Lys Leu Ile Leu Thr Ala Arg
35 40 45
Arg Leu Glu Lys Leu Ala Ala Ile Lys Glu Glu Met Lys Lys Glu Tyr
50 55 60
Pro Asn Val Lys Ile His Val Ala Lys Leu Asp Val Ser Asn Thr Glu
65 70 75 80
Glu Ile Lys Ser Leu Leu Gln Cys Leu Pro Thr Glu Phe Lys Ser Ile
85 90 95
Asp Ile Leu Val Asn Asn Ala Gly Lys Ala Leu Gly Ser Glu Lys Val
100 105 110
Gly Asp Ile Ala Ile Glu Asp Ile Lys Gly Met Met Asp Thr Asn Val
115 120 125
Ile Gly Leu Ile Asn Val Thr Gln Ala Val Leu Pro Ile Leu Lys Glu
130 135 140
Lys Asn Ser Gly Asp Ile Val Asn Ile Gly Ser Val Ala Gly Arg Asp
145 150 155 160
Ala Tyr Pro Thr Gly Ser Ile Tyr Cys Ala Ser Lys Phe Ala Val Arg
165 170 175
Ala Phe Thr Glu Ser Leu Arg Lys Glu Leu Leu Asp Thr Gly Ile Arg
180 185 190
Val Ile Leu Ile Ala Pro Gly Leu Val Glu Thr Glu Phe Ser Leu Val
195 200 205
Arg Tyr Lys Gly Asp Glu Lys Arg Ala Asn Ser Val Tyr Glu Gly Ile
210 215 220
Thr Ala Leu Glu Ala Glu Asp Val Ala Glu Leu Ile Val Phe Ser Thr
225 230 235 240
Ser Arg Lys Pro Asn Thr Val Ile Ala Asp Thr Leu Ile Phe Pro Thr
245 250 255
Cys Gln Gly Ser Ala Phe His Val His Arg Ser
260 265
<210> 136
<211> 314
<212> PRT
<213> HPDHms
<400> 136
Met Thr Glu Lys Val Ser Val Val Gly Ala Gly Val Ile Gly Val Gly
1 5 10 15
Trp Ala Thr Leu Phe Ala Ser Lys Gly Tyr Ser Val Ser Leu Tyr Thr
20 25 30
Glu Lys Lys Glu Thr Leu Asp Lys Gly Ile Glu Lys Leu Arg Asn Tyr
35 40 45
Val Gln Val Met Lys Asn Asn Ser Gln Ile Thr Glu Asp Val Asn Thr
50 55 60
Val Ile Ser Arg Val Ser Pro Thr Thr Asn Leu Asp Glu Ala Val Arg
65 70 75 80
Gly Ala Asn Phe Val Ile Glu Ala Val Ile Glu Asp Tyr Asp Ala Lys
85 90 95
Lys Lys Ile Phe Gly Tyr Leu Asp Ser Val Leu Asp Lys Glu Val Ile
100 105 110
Leu Ala Ser Ser Thr Ser Gly Leu Leu Ile Thr Glu Val Gln Lys Ala
115 120 125
Met Ser Lys His Pro Glu Arg Ala Val Ile Ala His Pro Trp Asn Pro
130 135 140
Pro His Leu Leu Pro Leu Val Glu Ile Val Pro Gly Glu Lys Thr Ser
145 150 155 160
Met Glu Val Val Glu Arg Thr Lys Ser Leu Met Glu Lys Leu Asp Arg
165 170 175
Ile Val Val Val Leu Lys Lys Glu Ile Pro Gly Phe Ile Gly Asn Arg
180 185 190
Leu Ala Phe Ala Leu Phe Arg Glu Ala Val Tyr Leu Val Asp Glu Gly
195 200 205
Val Ala Thr Val Glu Asp Ile Asp Lys Val Met Thr Ala Ala Ile Gly
210 215 220
Leu Arg Trp Ala Phe Met Gly Pro Phe Leu Thr Tyr His Leu Gly Gly
225 230 235 240
Gly Glu Gly Gly Leu Glu Tyr Phe Phe Asn Arg Gly Phe Gly Tyr Gly
245 250 255
Ala Asn Glu Trp Met His Thr Leu Ala Lys Tyr Asp Lys Phe Pro Tyr
260 265 270
Thr Gly Val Thr Lys Ala Ile Gln Gln Met Lys Glu Tyr Ser Phe Ile
275 280 285
Lys Gly Lys Thr Phe Gln Glu Ile Ser Lys Trp Arg Asp Glu Lys Leu
290 295 300
Leu Lys Val Tyr Lys Leu Val Trp Glu Lys
305 310
<210> 137
<211> 550
<212> PRT
<213> HPDHot
<400> 137
Met Phe Met Thr Arg Leu Asn Asp Lys Ile Ala Leu Ile Thr Gly Gly
1 5 10 15
Ala Gly Thr Ile Gly Glu Val Ile Thr Arg Arg Tyr Leu Glu Glu Gly
20 25 30
Ala Thr Val Val Met Ala Gly Arg Asn Arg Asp Lys Leu Asp Arg Tyr
35 40 45
Arg Glu Arg Leu Ile Thr Glu Phe His Ala Leu Pro Glu Arg Val Met
50 55 60
Val Val Arg Met Asp Gly Ser Ser Asn Ala Glu Val Arg Met Gly Ile
65 70 75 80
Ala Ala Val Val Ala His Phe Gly Arg Ile Asp Ile Leu Val Asn Asn
85 90 95
Ala Gly Ser Ala Gly Ala Arg Gln Arg Leu Pro Ala Ile Pro Leu Leu
100 105 110
Arg Ser Glu Leu Gln Ala Asp Glu Thr Glu Thr Leu Ala Asp Ser Ile
115 120 125
Gly Asn Leu Ile Gly Ile Thr Trp Asn Leu Ile Arg Ala Ala Ala Pro
130 135 140
Phe Met Pro Ala Gly Ser Ser Val Ile Asn Ile Ser Thr Ile Phe Ala
145 150 155 160
Arg Thr Asp Tyr Tyr Gly Arg Ile Pro Tyr Val Val Pro Lys Ala Ala
165 170 175
Leu His Ala Leu Thr Leu Ala Ala Ala Thr Glu Leu Gly Glu Arg Gly
180 185 190
Ile Arg Val Asn Gln Ile Asn Pro Gly Pro Ile Asp Ser Asp Arg Ile
195 200 205
Arg Thr Val Phe Arg Arg Met Asp Glu Leu Lys Gly Val Pro Glu Gln
210 215 220
Ser Thr Ala Asp Gly Phe Phe Gln Met Met Arg Leu Arg Arg Pro Asn
225 230 235 240
Ala Glu Gly Asp Leu Val Lys Gly Phe Pro Lys Thr Leu Asp Val Ala
245 250 255
Asn Val Ala Val Phe Leu Gly Ser Ala Glu Ser Ala Ala Leu Ser Gly
260 265 270
Glu Thr Leu Asp Val Thr His Gly Met Ala Val Pro Thr Glu Ser Arg
275 280 285
Thr Thr Leu Thr Ser Arg Pro Gly Leu Arg Ala Val Asp Gly Ser Gly
290 295 300
His Thr Thr Leu Ile Cys Val Gly Asp Gln Ile Glu Glu Ala Ala Ala
305 310 315 320
Leu Thr Gly Val Leu Arg Ala Cys Gly Ala Glu Val Val Ile Gly Phe
325 330 335
Arg Ser Arg Ala Ala Ile Ala Arg Phe Asp His Leu Ile Glu Arg Gly
340 345 350
Arg His Leu Pro Ser Gln Glu His Val Ala Pro Val Leu Leu Tyr Leu
355 360 365
Asn Pro Thr Glu Pro Glu Ser Ile Asp Gln Ala Leu Arg Trp Met Ala
370 375 380
Thr Asn Leu Asp Leu Pro Thr Ser Val Ile Ile Leu Pro Ala Gln Arg
385 390 395 400
Gln Pro Leu Pro Pro Ser Val Val Arg Ala Ser Asp Glu Glu Val Ala
405 410 415
Tyr Phe Leu Arg Asp Glu Leu Ser Gly Met Ile Val Leu Ala Ser Arg
420 425 430
Leu Ala Arg Phe Trp Gln Gln Ala Thr Leu Ala Pro Gly Asn Ala Pro
435 440 445
Ile Gln Pro Arg Val Leu Phe Met Thr Asn Pro Asp Asp Gly Gln Gly
450 455 460
Asn Leu Tyr Ala Glu Ile Leu Arg Ala Gly Val Glu Gln Leu Cys Arg
465 470 475 480
Val Trp Arg His Glu Ser Gln Leu Asp Tyr Thr Arg Leu Ala Gln Met
485 490 495
Asp Ala His Pro Pro His Ile Arg Pro Val Trp Ala Asn Gln Leu Val
500 505 510
Arg Phe Ala Asn Asn Glu Gln Glu Asn Leu Glu Tyr Cys Cys Ala Trp
515 520 525
Val Ala Lys Ile Leu Leu Ser Glu Arg Thr Ile Glu Glu Leu Asn Leu
530 535 540
Tyr Leu Pro Arg Gln Ile
545 550
<210> 138
<211> 271
<212> PRT
<213> HPDHps
<400> 138
Met Ser Tyr Gly Ser Lys Ala Ala Glu Arg Leu Ala Asn Lys Val Ile
1 5 10 15
Leu Ile Thr Gly Ala Ser Ala Ala Ala Gly Ile Gly Ala Ala Thr Ala
20 25 30
Arg Glu Phe Ala Glu Ala Ala His Gly Asn Ile Lys Leu Ile Leu Thr
35 40 45
Ala Arg Arg Lys Asp Lys Leu Asp Ala Leu Ala Lys Glu Leu Thr Asp
50 55 60
Lys Tyr Asp Asn Ile Lys Val His Thr Val Ala Leu Asp Val Thr Lys
65 70 75 80
Leu Glu Ser Ile Gly Gln Phe Val Lys Asp Leu Pro Gln Glu Phe Ala
85 90 95
Asp Ile Asp Val Leu Val Asn Asn Ala Gly Leu Ala Leu Gly Arg Asp
100 105 110
Pro Ile Gly Glu Ile Leu Pro Glu Asp Ile Gln Gly Met Phe Gln Thr
115 120 125
Asn Val Leu Gly Leu Ile Thr Leu Thr Gln Ala Val Leu Pro Ile Phe
130 135 140
Lys Ala Lys Asn Ser Gly Asp Ile Val Asn Leu Gly Ser Ile Ala Gly
145 150 155 160
Arg Asp Pro Tyr Pro Gly Gly Gly Ile Tyr Cys Pro Thr Lys Ala Ala
165 170 175
Val Lys Ser Phe Ser His Val Leu Arg Lys Glu Leu Ile Asn Thr Arg
180 185 190
Ile Arg Val Leu Glu Val Asp Pro Gly Asn Val Glu Thr Glu Phe Ser
195 200 205
Ile Thr Arg Phe Lys Asn Asp Val Asp Lys Ala Lys Ala Val Tyr Ala
210 215 220
Asn Thr Glu Pro Leu Val Ala Glu Asp Ile Ala Glu Val Ile Val Phe
225 230 235 240
Gly Ile Thr Arg Lys Gln Asn Thr Val Ile Ala Glu Thr Leu Ile Phe
245 250 255
Ser Thr Asn Gln Ala Ser Ala Ser His Leu Tyr Lys Gly Gln Val
260 265 270
<210> 139
<211> 249
<212> PRT
<213> HPDHra
<400> 139
Met Ile Ile Phe Val Thr Gly Ala Thr Ala Gly Phe Gly Ala Glu Ile
1 5 10 15
Ala Arg Lys Phe Val Lys Glu Gly His Lys Val Ile Ala Thr Gly Arg
20 25 30
Arg Lys Glu Arg Leu Asp Asp Leu Lys Ala Glu Leu Gly Asp Ala Leu
35 40 45
Tyr Ala Val Glu Leu Asp Val Thr Asn Arg Ala Ser Ile Glu Glu Ala
50 55 60
Ile Ala Ser Leu Pro Ala Asp Trp Gln Asn Ile Asp Val Leu Val Asn
65 70 75 80
Asn Ala Gly Leu Ala Leu Gly Leu Glu Lys Ala Asp Lys Ala Ser Leu
85 90 95
Glu Asp Trp Asp Lys Met Ile Asp Thr Asn Thr Lys Gly Leu Val Tyr
100 105 110
Met Thr Arg Ala Val Leu Pro Gly Met Val Glu Arg Asn Thr Gly His
115 120 125
Val Ile Asn Ile Gly Ser Thr Ala Gly Asn Trp Pro Tyr Ala Gly Gly
130 135 140
Asn Val Tyr Gly Ala Thr Lys Ala Phe Val Arg Gln Phe Ser Leu Asn
145 150 155 160
Leu Arg Thr Asp Leu His Gly Thr His Val Arg Val Thr Asn Ile Glu
165 170 175
Pro Gly Leu Cys Gly Gly Thr Glu Phe Ser Asn Val Arg Phe Lys Gly
180 185 190
Asp Asp Glu Lys Val Ser Lys Thr Tyr Asp Asn Thr Asn Pro Leu Thr
195 200 205
Ala Ala Asp Val Ala Glu Ser Val Phe Trp Ala Ser Thr Leu Pro Ala
210 215 220
His Val Asn Ile Asn Ser Ile Glu Met Met Pro Val Ser Gln Ser Leu
225 230 235 240
Ala Gly Leu Gln Val His Arg Glu Gly
245
<210> 140
<211> 550
<212> PRT
<213> HPDHrc
<400> 140
Met Ser Thr Val Arg Arg Leu Glu Gly Lys Val Ala Leu Ile Thr Gly
1 5 10 15
Gly Ala Gly Asn Ile Gly Glu Val Ile Thr Arg Arg Phe Leu Ala Glu
20 25 30
Gly Ala Thr Val Val Ile Thr Gly Arg Asn Ala Glu Lys Leu Ala Val
35 40 45
Tyr Arg Arg Arg Leu Ile Asp Glu Glu Arg Val Ala Pro Glu Arg Val
50 55 60
Val Ala Leu Arg Met Asp Gly Ser Asp Ile Ala Gln Val Arg Ala Gly
65 70 75 80
Val Ala Gln Ile Val His Gly Gly Thr Asp Val Pro Ile Pro Leu His
85 90 95
Arg Ile Asp Ile Leu Val Asn Asn Ala Gly Ser Ala Gly Pro Arg Arg
100 105 110
Arg Leu Val Asp Ile Pro Leu Glu Pro Ser Glu Val Gln Pro Pro Asp
115 120 125
Ser Glu Thr Leu Ala Gln Ala Val Gly Asn Leu Val Gly Ile Thr Trp
130 135 140
Asn Leu Thr Arg Ala Ala Ala Pro His Met Pro Ser Gly Ser Ser Val
145 150 155 160
Ile Asn Ile Ser Thr Ile Phe Ser Arg Thr Asp Tyr Tyr Gly Arg Ile
165 170 175
Ala Tyr Val Ala Pro Lys Ala Ala Leu Asn Ala Leu Ser Asp Gly Leu
180 185 190
Ala Arg Glu Leu Gly Val Arg Gly Ile Arg Val Asn Thr Ile Tyr Pro
195 200 205
Gly Pro Ile Glu Ser Glu Arg Ile Tyr Thr Met Phe Gln Ala Met Asp
210 215 220
Ala Leu Lys Gly Gln Pro Glu Gly Asp Thr Ala Ser Gly Phe Leu Arg
225 230 235 240
Met Met Arg Leu Ser Arg Ile Asp Gln Asn Gly Glu Val Val Lys Arg
245 250 255
Phe Pro Ser Pro Val Asp Val Ala Asn Thr Ala Val Phe Leu Ala Ser
260 265 270
Asp Glu Ser Ala Ala Phe Thr Gly His Ala Phe Glu Val Thr His Gly
275 280 285
Met Glu Val Pro Thr Glu Ser Arg Thr Thr Phe Val Ser Arg Pro Gly
290 295 300
Leu Arg Ser Val Asp Ala Thr Gly Lys Val Ile Leu Ile Cys Ala Gly
305 310 315 320
Asp Gln Val Asp Asp Ala Val Ala Leu Ala Asp Thr Leu Arg Ser Cys
325 330 335
Arg Ala Thr Val Val Ile Gly Phe Arg Asp Pro Arg Ala Leu Glu Lys
340 345 350
Ala Ser Val Leu Leu Arg Glu Pro Arg His Ala Leu Ala Ala Asp Met
355 360 365
Tyr Gly Arg Pro Thr Met Thr Ala Glu Ala Arg Leu Val Arg Leu Asp
370 375 380
Pro Leu Asp Pro Arg Ala Ala Ala Gln Thr Leu Glu Gln Ile His Ala
385 390 395 400
Glu Leu Gly Ala Ile His His Ala Val Val Leu Pro Gly Gln Ser Arg
405 410 415
His Ala Pro Ser Ala Ser Leu Ile Glu Val Asp Asp Gln Val Val Glu
420 425 430
Arg Phe Leu His Gln Glu Leu Val Gly Thr Ile Ala Leu Ala Arg Glu
435 440 445
Leu Ala Arg Phe Trp Glu Glu Tyr Pro Ser Gly Ser Ser Met His Arg
450 455 460
Val Leu Phe Val Ser Asn Pro Asp Asp Gln Gln Gly Asn Gln Tyr Ser
465 470 475 480
His Ile Leu Arg Ala Ala Val Glu Gln Leu Val Arg Val Trp Arg His
485 490 495
Glu Ser Glu Tyr Asp Ser Val Asn Pro Ala His Gln Gln Glu Gly Gln
500 505 510
Ser Ser Ala Ala Val Trp Ala Asn Gln Leu Ile Arg Tyr Val Asn Asn
515 520 525
Glu Met Ala Asn Leu Asp Phe Thr Cys Ala Trp Val Ala Lys Leu Leu
530 535 540
Gly Ser Asp Arg Arg Ile
545 550
<210> 141
<211> 322
<212> PRT
<213> HPDHsi
<400> 141
Met Phe Ile His Met Lys Ser Ile Asn Lys Val Ala Val Val Gly Ala
1 5 10 15
Gly Ile Ile Gly Val Gly Trp Thr Thr Leu Leu Leu Thr Lys Gly Tyr
20 25 30
Lys Val Asn Leu Tyr Thr Glu Lys Lys Glu Thr Leu Glu Lys Gly Leu
35 40 45
Ala Lys Val Ser Ala Tyr Leu Val Asn Leu Lys Asn Leu Arg Met Ile
50 55 60
Asp Gly Glu Pro Ile Asp Tyr Gln Arg Asn Leu Thr Gly Thr Thr Lys
65 70 75 80
Ile Asp Glu Ala Ile Gln Asn Val Glu Phe Val Ile Glu Ala Ile Ile
85 90 95
Glu Asp Tyr Ser Ala Lys Lys Asn Leu Phe Asn Tyr Leu Asp Ser Gln
100 105 110
Leu Pro Arg Asp Ile Ile Ile Ala Ser Ser Thr Ser Gly Leu Leu Met
115 120 125
Thr Glu Ile Gln Lys Ala Met Thr Arg Tyr Pro Asp Arg Gly Val Ile
130 135 140
Ala His Pro Trp Asn Pro Pro His Leu Leu Pro Leu Val Glu Ile Val
145 150 155 160
Pro Gly Asp Lys Thr Ser Lys Tyr Thr Leu Asp Val Thr Lys Asp Phe
165 170 175
Met Glu Lys Leu Asp Arg Val Val Val Val Leu Lys Lys Glu Val Pro
180 185 190
Gly Phe Ile Gly Asn Arg Leu Ala Phe Ala Leu Phe Arg Glu Ala Val
195 200 205
Asn Leu Ile Asp Glu Gly Val Ala Thr Val Glu Asp Ile Asp Lys Val
210 215 220
Met Thr Ala Ala Ile Gly Leu Arg Trp Ala Phe Met Gly Pro Phe Leu
225 230 235 240
Thr Tyr His Leu Gly Gly Gly Glu Gly Gly Ile Glu Tyr Phe Phe Ser
245 250 255
Lys Gly Phe Gly Tyr Gly Ala Asn Glu Trp Met Tyr Thr Leu Ala Lys
260 265 270
Trp Asp Lys Phe Pro Tyr Thr Ala Val Lys Lys Ile Val Asp Gln Met
275 280 285
Lys Glu Tyr Glu Phe Ile Lys Gly Lys Asn Phe Gln Asp Leu Ser Arg
290 295 300
Trp Arg Asp Glu Thr Leu Val Arg Val Tyr Lys Ala Val Phe Gly Glu
305 310 315 320
Lys Arg
<210> 142
<211> 550
<212> PRT
<213> HPDHsl
<400> 142
Met Asn Ala Met Gly Lys Thr Glu Thr Gln Gly Arg Leu Ala Gly Lys
1 5 10 15
Val Ala Leu Ile Thr Gly Ala Ala Gly Asn Leu Gly Gly Ser Ile Val
20 25 30
Arg Arg Tyr Leu Ala Glu Gly Ala Thr Val Val Met Ser Gly Arg Asp
35 40 45
Ala Ala Arg Thr Gln Ala Ala Leu Asp Ala Ala Val Ala Asp Val Gly
50 55 60
Gly Thr Ala Ser Ile Val Thr Met Asp Gly Ala Asn Ile Glu Ser Val
65 70 75 80
Arg Ala Gly Ile Ala Asp Val Val Ala Arg His Gly Arg Ile Asp Val
85 90 95
Leu Val Asn Asn Ala Gly Ser Ala Gly Pro Lys Gln Val Leu Gly Asp
100 105 110
Leu Pro Leu Leu Pro Glu Glu Leu Glu Gly Thr Pro Asp Ser Glu Thr
115 120 125
Val Gly Asp Ala Ala Arg Asn Ile Met Val Val Ala Trp Asn Leu Val
130 135 140
Arg Ala Ala Ala Ser Ala Met Gly Glu Gly Gly Ser Ile Ile Asn Val
145 150 155 160
Ser Thr Ile Phe Ser Arg Thr Asp Tyr Phe Gly Arg Ala Ala Tyr Thr
165 170 175
Val Pro Lys Ala Ala Leu Asn Ile Leu Ser Lys Gly Ile Ala Arg Glu
180 185 190
Leu Gly Thr Arg Gly Ile Arg Val Asn Gln Leu Tyr Pro Gly Pro Ile
195 200 205
Ala Ser Glu Arg Ile Arg Thr Val Phe Ala Ala Met Asp Lys Leu Lys
210 215 220
Gly Asp Asp Ala Gly Ser Thr Ala Asn His Phe Phe Asn Leu Met Ala
225 230 235 240
Leu Glu Arg Ser Val Gly Glu Asp Ala Lys Ala Lys Thr Phe Pro Thr
245 250 255
Pro Ala Asp Ile Ala Asn Thr Cys Val Phe Leu Gly Ser Asp Glu Ser
260 265 270
Ala Ala Phe Ala Ala His Asp Phe Glu Val Thr His Gly Met Ala Val
275 280 285
Pro Leu Glu Ser Lys Ser Glu Phe Leu His Arg Pro Asp Leu Arg Ala
290 295 300
Ile Asp Gly Asp Gly His Gly Ile Leu Ile Ser Ala Gly Asn Gln Leu
305 310 315 320
Asp Asp Ala Leu Ala Met Ala Gly Leu Phe Ala Ala Gln Gly Ser Lys
325 330 335
Val Leu Leu Gly Phe His Ala Gln Ala Ala Leu Asp Asp Ala Ala Glu
340 345 350
Arg Leu Pro Ala Gly Val Lys Ala Val Arg Phe Pro Arg His Asp His
355 360 365
Met Ala Met Asp Ala Glu Leu Ala Arg Phe Thr Ala His Val Ala Ala
370 375 380
Ile Asp Gly Ala Ile Ile Leu Pro Thr Arg Gly Pro Gly Tyr Phe His
385 390 395 400
Gly Ala Leu Asn Glu Ala Ser Asp Ala Ala Val Glu Arg Phe Val Asp
405 410 415
Asp Glu Leu Glu Gly Ala Ile Ala Val Ala Arg Thr Leu Asp Arg Tyr
420 425 430
Trp Gln Gln Gly Gln Asp Leu Pro Gly Asp Pro Arg Val Leu Phe Ile
435 440 445
Thr Asn Pro Asp Asp Gly Ala Gly Asn Gly Trp Ala Asp Val Gly Arg
450 455 460
Ala Ala Ile Glu Glu Leu Leu Arg Val Trp Arg Asp Glu Ala Arg Val
465 470 475 480
Ala Ala Thr Gln Gly Lys Thr Pro Arg Pro Val Trp Gly Asn Gln Ile
485 490 495
Val Arg Phe Gly Asn Asn Glu Pro Glu Asn Arg Asp Phe Ala Ala Gly
500 505 510
His Ala Ala Arg Leu Leu Leu Gly Gly Arg Gln Ile Glu Pro Ile Asn
515 520 525
Leu Tyr Ile Pro Ala Ser Ile Thr Lys Ala Thr Gly Ala Arg Arg Ala
530 535 540
Leu Ala Gly Thr Ala Glu
545 550
<210> 143
<211> 288
<212> PRT
<213> HPDHsm
<400> 143
Met Ala Ile Leu Ile Thr Gly Ala Ser Ser Gly Phe Gly Ala Ala Met
1 5 10 15
Ala Thr Ala Phe Val Gln Ala Gly Tyr Leu Val Ile Gly Thr Ala Arg
20 25 30
Arg Thr Glu Lys Leu Ala Asp Leu His Ala Lys Leu Gly Asp Arg Phe
35 40 45
Gln Pro Ile Thr Met Asp Met Thr Ser Lys Asp Asp Ile Gln Ala Ala
50 55 60
Leu Ala His Ile Gln Thr Leu Pro Glu Tyr Phe Arg Glu Ile Asp Cys
65 70 75 80
Leu Ile Asn Asn Ala Gly Leu Ala Leu Gly Leu Asp Arg Ala Asp Gln
85 90 95
Ala Asp Phe Thr Asp Trp Glu Thr Met Ile Gln Thr Asn Ile Ile Gly
100 105 110
Leu Thr Tyr Leu Thr Arg Gln Ile Leu Pro Lys Met Val Glu Asn Lys
115 120 125
Arg Gly Tyr Ile Ile Asn Met Gly Ser Ile Ala Gly Asn Tyr Pro Tyr
130 135 140
Ala Gly Ser Asn Val Tyr Gly Ala Thr Lys Ala Tyr Val Arg Gln Phe
145 150 155 160
Ser Leu Asn Leu Arg Ala Asp Leu His Gly Thr Gly Val Arg Val Thr
165 170 175
Asn Ile Glu Pro Gly Leu Val Glu Gly Ser Glu Phe Ser Asn Val Arg
180 185 190
Phe Lys Gly Asp Asp Lys Arg Ala Ala Gln Leu Tyr Glu Tyr Ala Asn
195 200 205
Ala Leu Thr Ser Glu Asp Ile Ala Gln Thr Ala Leu Trp Leu Tyr Gln
210 215 220
Arg Pro Ala His Met Asn Val Asn Thr Ile Glu Leu Met Pro Thr Thr
225 230 235 240
Gln Ser Trp Ala Gly Met Gln Val Tyr Lys Asp Thr Pro Pro Pro Pro
245 250 255
Pro Pro Val Val Glu Pro Lys Pro Ala Asp Phe Ala Pro Gln Pro Ala
260 265 270
Pro Gln Glu Glu Ser Gly Phe Phe Ser Lys Ile Thr Ser Leu Phe Lys
275 280 285
<210> 144
<211> 318
<212> PRT
<213> HPDHst
<400> 144
Met Arg Gln Ile Lys Thr Val Ser Val Ile Gly Ala Gly Val Ile Gly
1 5 10 15
Ala Gly Trp Ser Thr Leu Leu Ala Leu Lys Gly Tyr Glu Asn Trp Phe
20 25 30
Tyr Thr Glu Lys Lys Glu Thr Leu Asp Lys Gly Leu Ala Lys Ile Lys
35 40 45
Gly Tyr Leu Asn Val Leu Tyr Glu Tyr Lys Leu Ile Asp Lys Glu Pro
50 55 60
Asp Tyr Tyr Met Gln Arg Ile His Pro Thr Thr Lys Leu Asp Glu Ala
65 70 75 80
Ile Ser Asn Thr Asp Phe Val Leu Glu Ala Ile Val Glu Glu Tyr Gly
85 90 95
Ala Lys Lys Ala Leu Phe Lys Gln Leu Asp Glu Lys Leu Asp Lys Asp
100 105 110
Val Ile Leu Ala Ser Ser Thr Ser Gly Leu Leu Met Thr Glu Ile Gln
115 120 125
Lys Ala Met Thr Lys Tyr Pro Glu Arg Gly Ile Ile Ala His Pro Trp
130 135 140
Asn Pro Pro His Leu Leu Pro Leu Val Glu Ile Val Pro Gly Glu Lys
145 150 155 160
Thr Ser Gln Asp Thr Ile Tyr Ala Thr Lys Asp Phe Met Glu Asn Lys
165 170 175
Leu Glu Arg Val Val Val Val Leu Lys Lys Glu Ile Ser Gly Phe Ile
180 185 190
Gly Asn Arg Leu Ala Phe Ala Leu Phe Arg Glu Ala Val Tyr Leu Val
195 200 205
Asp Glu Gly Ile Ala Thr Val Glu Asp Ile Asp Lys Val Met Thr Ala
210 215 220
Ala Ile Gly Leu Arg Trp Ala Phe Met Gly Pro Phe Leu Thr Tyr His
225 230 235 240
Leu Gly Gly Gly Glu Gly Gly Leu Glu Tyr Phe Phe Ser Arg Gly Phe
245 250 255
Gly Tyr Gly Ala Asn Glu Trp Met His Thr Leu Ala Lys Tyr Asp Lys
260 265 270
Phe Pro Tyr Thr Gly Val Val Lys Ser Val Gln Gln Met Lys Glu Tyr
275 280 285
Glu Phe Val Lys Ser Lys Thr Phe Gln Glu Leu Ser Arg Trp Arg Asp
290 295 300
Glu Lys Leu Ile Ser Leu Ile Lys Leu Leu Arg Gly Lys Ile
305 310 315

Claims (13)

1.重组酵母，其包含[丙酮酸→乙醛→乙酰辅酶A→丙二酰辅酶A→丙二酸半醛→3-HP]的主动3-HP生物合成途径，其中所述酵母包含：
编码AADH的外源基因；
编码ACC的内源基因或外源基因；
编码MCR的外源基因；和
编码HPDH的外源基因。
2.根据权利要求1所述的重组酵母，其中所述编码AADH的基因为编码下述AADH的核酸：AADH具有与选自表1-3中所示的氨基酸序列的AADH氨基酸序列具有至少80％的序列同一性的氨基酸序列。
3.根据权利要求1所述的重组酵母，其中所述编码ACC的基因为编码下述ACC的核酸：ACC具有与选自表4所示的氨基酸序列的ACC氨基酸序列具有至少80％的序列同一性的氨基酸序列。
4.根据权利要求1所述的重组酵母，其中所述MCR是双功能的，所述MCR同时具有将丙二酰辅酶A转化为丙二酸半醛的功能和将丙二酸半醛转化为3-HP的功能。
5.根据权利要求4所述的重组酵母，其中编码所述双功能MCR的基因为编码下述MCR的核酸：MCR具有与选自表5所示的氨基酸序列的MCR氨基酸序列具有至少80％的序列同一性的氨基酸序列。
6.根据权利要求1所述的重组酵母，其中所述MCR是单功能的，所述MCR具有将丙二酰辅酶A转化为丙二酸半醛的功能；并且还包含编码将丙二酸半醛转化为3-HP的酶的基因。
7.根据权利要求6所述的重组酵母，其中编码所述MCR的基因为编码下述MCR的核酸：MCR具有与选自表6所示的氨基酸序列的MCR氨基酸序列具有至少80％的序列同一性的氨基酸序列。
8.根据权利要求6所述的重组酵母，其中所述编码将丙二酸半醛转化为3-HP的酶的基因为编码下述酶的核酸：所述酶具有与选自表7-10中所示的氨基酸序列的HPDH、HIBADH、HBDH或BDH氨基酸序列具有至少80％的序列同一性的氨基酸序列。
9.根据权利要求1所述的重组酵母，其中所述酵母是耐酸的。
10.根据权利要求9所述的重组酵母，其中所述酵母选自酿酒酵母(Saccharomycescerevisiae)、Kazachstania exigua、Kazachstania bulderi和小假丝酵母(Candidahumilis)。
11.一种制备3-HP的方法，其包括：
(a)在包含至少一种碳源的培养基中培养权利要求1-10中任一项所述的重组酵母，从而产生3-HP；以及
(b)从培养物中分离3-HP。
12.根据权利要求11所述的制备3-HP的方法，其中所述碳源为选自葡萄糖、木糖、阿拉伯糖、蔗糖、果糖、半乳糖、纤维素、葡萄糖低聚物和甘油中的一种或多种。
13.根据权利要求11所述的制备3-HP的方法，其中培养在2.6-4.0的pH下进行。
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