KR101808496B1 - Adamts 단백질 발현을 위한 세포 배양 배지 - Google Patents

Adamts 단백질 발현을 위한 세포 배양 배지 Download PDF

Info

Publication number
KR101808496B1
KR101808496B1 KR1020177018298A KR20177018298A KR101808496B1 KR 101808496 B1 KR101808496 B1 KR 101808496B1 KR 1020177018298 A KR1020177018298 A KR 1020177018298A KR 20177018298 A KR20177018298 A KR 20177018298A KR 101808496 B1 KR101808496 B1 KR 101808496B1
Authority
KR
South Korea
Prior art keywords
culture
adamts13
protein
culture medium
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020177018298A
Other languages
English (en)
Korean (ko)
Other versions
KR20170081747A (ko
Inventor
레오폴트 그릴베르거
알렉산드라 스펜거
마인하르트 하슬라허
라나 그릴베르거
만프레트 라이터
Original Assignee
백스터 인터내셔널 인코포레이티드
박스터 헬쓰케어 에스에이
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 백스터 인터내셔널 인코포레이티드, 박스터 헬쓰케어 에스에이 filed Critical 백스터 인터내셔널 인코포레이티드
Publication of KR20170081747A publication Critical patent/KR20170081747A/ko
Application granted granted Critical
Publication of KR101808496B1 publication Critical patent/KR101808496B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
KR1020177018298A 2009-07-31 2010-07-30 Adamts 단백질 발현을 위한 세포 배양 배지 Active KR101808496B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31
US61/230,477 2009-07-31
PCT/US2010/044020 WO2011014838A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020157033595A Division KR101756354B1 (ko) 2009-07-31 2010-07-30 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020177035177A Division KR101912552B1 (ko) 2009-07-31 2010-07-30 Adamts 단백질 발현을 위한 세포 배양 배지

Publications (2)

Publication Number Publication Date
KR20170081747A KR20170081747A (ko) 2017-07-12
KR101808496B1 true KR101808496B1 (ko) 2017-12-12

Family

ID=42668009

Family Applications (5)

Application Number Title Priority Date Filing Date
KR1020157009715A Active KR101574056B1 (ko) 2009-07-31 2010-07-30 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지
KR1020157033595A Active KR101756354B1 (ko) 2009-07-31 2010-07-30 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지
KR1020177035177A Active KR101912552B1 (ko) 2009-07-31 2010-07-30 Adamts 단백질 발현을 위한 세포 배양 배지
KR1020127005425A Active KR101573972B1 (ko) 2009-07-31 2010-07-30 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지
KR1020177018298A Active KR101808496B1 (ko) 2009-07-31 2010-07-30 Adamts 단백질 발현을 위한 세포 배양 배지

Family Applications Before (4)

Application Number Title Priority Date Filing Date
KR1020157009715A Active KR101574056B1 (ko) 2009-07-31 2010-07-30 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지
KR1020157033595A Active KR101756354B1 (ko) 2009-07-31 2010-07-30 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지
KR1020177035177A Active KR101912552B1 (ko) 2009-07-31 2010-07-30 Adamts 단백질 발현을 위한 세포 배양 배지
KR1020127005425A Active KR101573972B1 (ko) 2009-07-31 2010-07-30 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지

Country Status (21)

Country Link
US (7) US8313926B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP2459702B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (5) JP5451884B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
KR (5) KR101574056B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (2) CN104962539B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU2010278751B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BR (2) BR122021005965B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA2769361A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CO (1) CO6501191A2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EA (1) EA023193B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ES (1) ES2583258T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
HR (1) HRP20160783T1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
HU (1) HUE029110T2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2012DN00801A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
MX (2) MX2012001261A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
NZ (1) NZ597727A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
PL (1) PL2459702T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
PT (1) PT2459702T (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
SG (1) SG178194A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
SI (1) SI2459702T1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2011014838A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2769361A1 (en) * 2009-07-31 2011-02-03 Baxter International Inc. Cell culture medium for adamts protein expression
SI2591094T1 (sl) 2010-07-08 2018-12-31 Baxalta GmbH Metoda proizvodnje rekombinantnih ADAMTS13 v celični kulturi
IL315908A (en) 2011-07-01 2024-11-01 Amgen Inc Mammalian cell culture
ES2633960T3 (es) 2012-10-15 2017-09-26 Bristol-Myers Squibb Company Procesos de cultivo de células de mamífero para la producción de proteínas
WO2014159831A1 (en) 2013-03-13 2014-10-02 Merck Sharp & Dohme Corp. Adapted lepidopteran insect cells for the production of recombinant proteins
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
US10138467B2 (en) * 2013-10-14 2018-11-27 Ares Trading S.A. Medium for high performance mammalian fed-batch cultures
EP3926051B1 (en) 2014-06-04 2024-05-08 Amgen Inc. Methods for harvesting mammalian cell cultures
KR101867134B1 (ko) * 2015-03-23 2018-06-12 한양대학교 산학협력단 포유류 세포를 이용하여 목적 물질을 고효율로 생산하기 위한 세포 배양 배지, 이를 이용한 세포 배양 방법 및 목적 물질의 생산 방법
DK3298138T3 (da) * 2015-05-22 2022-10-31 Dupont Nutrition Biosci Aps Acetolactatdecarboxylase
TWI870789B (zh) 2015-08-04 2025-01-21 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
CN116159130B (zh) 2016-08-04 2025-09-09 武田药品工业株式会社 使用adamts13治疗、改善和/或预防镰状细胞病、急性肺损伤和/或急性呼吸窘迫综合征中的血管阻塞危象
AU2017391164B2 (en) * 2016-11-04 2024-01-04 Takeda Pharmaceutical Company Limited Adeno-associated virus purification methods
BR112020000127A2 (pt) * 2017-07-06 2020-07-07 Regeneron Pharmaceuticals, Inc. método para selecionar um hidrolisado de soja, e, glicoproteína
JP2021515584A (ja) * 2018-03-16 2021-06-24 ジェンザイム・コーポレーション 産生バイオリアクターにおける細胞生存度を改善するための方法
US20210189358A1 (en) * 2018-06-18 2021-06-24 Synthetic Biologics, Inc. Methods of making alkaline phosphatase agents
US20220339266A1 (en) 2019-10-04 2022-10-27 The Children's Medical Center Corporation Adamts13 treatment to enhance graft survival
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
AU2020398830C1 (en) 2019-12-06 2023-04-27 Regeneron Pharmaceuticals, Inc. Anti-VEGF protein compositions and methods for producing the same
JP2023521658A (ja) 2020-04-02 2023-05-25 武田薬品工業株式会社 Ａｄａｍｔｓ１３バリアント、組成物、及びその使用
MX2022013812A (es) 2020-05-08 2022-12-15 Regeneron Pharma Trampas y mini-trampas de vegf y metodos para el tratamiento de trastornos oculares y cancer.
JP2023526546A (ja) 2020-05-22 2023-06-21 武田薬品工業株式会社 コロナウイルス疾患の合併症の治療及び診断のための、ａｄａｍｔｓ１３組成物及び方法
WO2021246557A1 (ko) * 2020-06-05 2021-12-09 주식회사 프로앱텍 특정한 갯수의 알부민이 연결된 요산산화효소-알부민 복합체 및 이의 제조 방법
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5250421A (en) 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
CZ280901B6 (cs) 1988-10-06 1996-05-15 Hideaki Yamada Způsob kultivace bakterií
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
ATE429246T1 (de) 1994-11-10 2009-05-15 Baxter Healthcare Sa Verfahren zur herstellung von biologischen produkten in protein-freiem kultur
AU703484B2 (en) 1995-02-23 1999-03-25 Quest International Services B.V. Peptides for tissue and cell culture media
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
EP2218775B1 (en) 1996-08-30 2015-01-28 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
IT1285158B1 (it) * 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
DK0929507T3 (da) 1996-10-07 2003-03-24 Solutia Inc Fremgangsmåde til hydroxylering af benzen
EP1983044B1 (en) 1996-10-10 2016-08-10 Life Technologies Corporation Animal cell culture media comprising plant-derived nutrients
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US6926894B2 (en) 2000-11-22 2005-08-09 Baxter Aktiengesellschaft Composition exhibiting a von willebrand factor (vWF) protease activity comprising a polypeptide chain with the amino acid sequence AAGGILHLELLV
JP4723862B2 (ja) 2002-12-23 2011-07-13 フジフィルム マニュファクチャリング ユーロプ ビー．ブイ． 細胞培養支持体を被覆する方法
CN1969043A (zh) * 2004-04-16 2007-05-23 惠氏公司 Adamts－8蛋白及其用途
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
JP2007174948A (ja) * 2005-12-27 2007-07-12 Noritsu Koki Co Ltd 酵素水製造装置
JP4880997B2 (ja) * 2005-12-28 2012-02-22 アルフレッサファーマ株式会社 Ａｄａｍｔｓ１３の安定化方法
DK2522717T3 (da) 2006-01-04 2014-04-22 Baxter Int Oligopeptid-frie cellekulturmedier
BRPI0622214A2 (pt) * 2006-12-22 2012-01-03 Springer Carrier Ltda unidade de condicionamento de ar e mÉtodo de misturar ar em uma unidade de condicionamento de ar
US20090067805A1 (en) 2007-09-10 2009-03-12 Victor Company Of Japan, Limited Recording and reproducing device
KR101798813B1 (ko) * 2007-12-27 2017-11-16 박스알타 인코퍼레이티드 세포 배양 방법
CA2769361A1 (en) * 2009-07-31 2011-02-03 Baxter International Inc. Cell culture medium for adamts protein expression

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BLOOD. Vol. 100, No. 10, 페이지 3626-3632 (2002.11.15.)

Also Published As

Publication number Publication date
WO2011014838A1 (en) 2011-02-03
US8759026B2 (en) 2014-06-24
US10072254B2 (en) 2018-09-11
US20170051270A1 (en) 2017-02-23
JP6883071B2 (ja) 2021-06-09
KR101574056B1 (ko) 2015-12-02
JP2016144471A (ja) 2016-08-12
JP2019201661A (ja) 2019-11-28
EP2459702A1 (en) 2012-06-06
SG178194A1 (en) 2012-03-29
CN102549145A (zh) 2012-07-04
CN102549145B (zh) 2015-05-20
KR101756354B1 (ko) 2017-07-26
CN104962539A (zh) 2015-10-07
HRP20160783T1 (hr) 2016-08-12
JP2018093881A (ja) 2018-06-21
US20140329294A1 (en) 2014-11-06
KR101573972B1 (ko) 2015-12-02
ES2583258T3 (es) 2016-09-20
KR20170140411A (ko) 2017-12-20
JP5936232B2 (ja) 2016-06-22
HK1171790A1 (zh) 2013-04-05
PT2459702T (pt) 2016-08-02
US20190071660A1 (en) 2019-03-07
EA023193B1 (ru) 2016-05-31
JP7083059B2 (ja) 2022-06-09
KR101912552B1 (ko) 2018-10-26
KR20120046301A (ko) 2012-05-09
MX2012001261A (es) 2012-05-22
IN2012DN00801A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2015-06-26
US11254921B2 (en) 2022-02-22
AU2010278751A1 (en) 2012-02-09
CN104962539B (zh) 2019-09-17
JP6511007B2 (ja) 2019-05-08
US9127265B2 (en) 2015-09-08
US20150329848A1 (en) 2015-11-19
HUE029110T2 (en) 2017-02-28
JP5451884B2 (ja) 2014-03-26
CO6501191A2 (es) 2012-08-15
AU2010278751B2 (en) 2014-05-15
JP2021112210A (ja) 2021-08-05
KR20150138425A (ko) 2015-12-09
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
JP6567707B2 (ja) 2019-08-28
US20110086413A1 (en) 2011-04-14
KR20170081747A (ko) 2017-07-12
MX344970B (es) 2017-01-12
EP2459702B1 (en) 2016-05-04
CA2769361A1 (en) 2011-02-03
NZ597727A (en) 2013-08-30
BR122021005965B1 (pt) 2022-01-25
BR112012002151A2 (pt) 2020-03-03
BR112012002151B1 (pt) 2021-07-06
US8313926B2 (en) 2012-11-20
US20130071906A1 (en) 2013-03-21
PL2459702T3 (pl) 2016-12-30
US20200308566A1 (en) 2020-10-01
KR20150044981A (ko) 2015-04-27
US9441216B2 (en) 2016-09-13
JP2013500724A (ja) 2013-01-10
JP2014061005A (ja) 2014-04-10
EA201270224A1 (ru) 2012-09-28

Similar Documents

Publication Publication Date Title
KR101808496B1 (ko) Adamts 단백질 발현을 위한 세포 배양 배지
AU2017261523B2 (en) Cell culture medium for adamts protein expression
AU2019279943B2 (en) Cell culture medium for adamts protein expression
HK1171790B (en) Cell culture medium for adamts protein expression

Legal Events

Date Code Title Description
A107 Divisional application of patent
A201 Request for examination
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20170703

Application number text: 1020157033595

Filing date: 20151125

PA0201 Request for examination
PG1501 Laying open of application
E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20170906

A107 Divisional application of patent
GRNT Written decision to grant
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20171206

Application number text: 1020157033595

Filing date: 20151125

PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20171206

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20171206

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20201201

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20211122

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 20221123

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 20231128

Start annual number: 7

End annual number: 7