IN2012DN00801A - - Google Patents

Download PDF

Info

Publication number
IN2012DN00801A
IN2012DN00801A IN801DEN2012A IN2012DN00801A IN 2012DN00801 A IN2012DN00801 A IN 2012DN00801A IN 801DEN2012 A IN801DEN2012 A IN 801DEN2012A IN 2012DN00801 A IN2012DN00801 A IN 2012DN00801A
Authority
IN
India
Prior art keywords
adamts
expression
methods
useful
proteins
Prior art date
Application number
Other languages
English (en)
Inventor
Grillberger Leopold
Spenger Alexandra
Hasslacher Meinhard
Grillberger Rana
Reiter Manfred
Original Assignee
Baxter Int
Baxter Healthcare Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Int, Baxter Healthcare Sa filed Critical Baxter Int
Publication of IN2012DN00801A publication Critical patent/IN2012DN00801A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489Metalloendopeptidases (3.4.24)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0681Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0684Cells of the urinary tract or kidneys
    • C12N5/0686Kidney cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24087ADAMTS13 endopeptidase (3.4.24.87)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/12Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14Calcium; Ca chelators; Calcitonin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/22Zinc; Zn chelators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95Protein-free medium and culture conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/99Serum-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
IN801DEN2012 2009-07-31 2010-07-30 IN2012DN00801A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31
PCT/US2010/044020 WO2011014838A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Publications (1)

Publication Number Publication Date
IN2012DN00801A true IN2012DN00801A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2015-06-26

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
IN801DEN2012 IN2012DN00801A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2009-07-31 2010-07-30

Country Status (21)

Country Link
US (7) US8313926B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP2459702B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (5) JP5451884B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
KR (5) KR101912552B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (2) CN104962539B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU2010278751B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BR (2) BR122021005965B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA2769361A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CO (1) CO6501191A2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EA (1) EA023193B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ES (1) ES2583258T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
HR (1) HRP20160783T1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
HU (1) HUE029110T2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2012DN00801A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
MX (2) MX2012001261A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
NZ (1) NZ597727A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
PL (1) PL2459702T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
PT (1) PT2459702T (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
SG (1) SG178194A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
SI (1) SI2459702T1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2011014838A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2769361A1 (en) * 2009-07-31 2011-02-03 Baxter International Inc. Cell culture medium for adamts protein expression
DK3064508T4 (da) 2010-07-08 2023-04-11 Takeda Pharmaceuticals Co Fremgangsmåde til fremstilling af rekombinant vwf med høj molekylevægt i cellekultur
KR101857380B1 (ko) 2011-07-01 2018-05-11 암젠 인크 포유동물 세포 배양
EP2906683B1 (en) 2012-10-15 2017-05-31 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
US9611455B2 (en) 2013-03-13 2017-04-04 Merck Sharp & Dohme Corp. Adapted lepidopteran insect cells for the production of recombinant proteins
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (es) * 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
CA2926517C (en) * 2013-10-14 2021-03-23 Ares Trading S.A. New medium for high performance mammalian fed-batch cultures
ES2709994T3 (es) 2014-06-04 2019-04-22 Amgen Inc Métodos de recolección en cultivos de células de mamífero
KR101867134B1 (ko) * 2015-03-23 2018-06-12 한양대학교 산학협력단 포유류 세포를 이용하여 목적 물질을 고효율로 생산하기 위한 세포 배양 배지, 이를 이용한 세포 배양 방법 및 목적 물질의 생산 방법
MX2017014595A (es) * 2015-05-22 2018-03-21 Dupont Nutrition Biosci Aps Acetolactato-descarboxilasa.
TWI870789B (zh) 2015-08-04 2025-01-21 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
KR102537083B1 (ko) 2016-08-04 2023-05-25 다케다 파머수티컬 컴패니 리미티드 겸상적혈구병, 급성 폐 손상 및/또는 급성 호흡 곤란 증후군에서 혈관폐색 위기의 치료, 개선, 및/또는 예방을 위한 adamts13의 용도
AU2017391164B2 (en) * 2016-11-04 2024-01-04 Takeda Pharmaceutical Company Limited Adeno-associated virus purification methods
SG11201912548XA (en) * 2017-07-06 2020-01-30 Regeneron Pharma Cell culture process for making a glycoprotein
CA3094076A1 (en) * 2018-03-16 2019-09-19 Genzyme Corporation Methods for improving cell viability in a production bioreactor
CA3103443A1 (en) * 2018-06-18 2019-12-26 Synthetic Biologics, Inc. Methods of making alkaline phosphatase agents
WO2021067668A1 (en) 2019-10-04 2021-04-08 The Children's Medical Center Corporation Adamts13 treatment to enhance graft survival
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
TW202128991A (zh) 2019-12-06 2021-08-01 美商再生元醫藥公司 抗vegf蛋白組成物及其製備方法
AU2021248679A1 (en) 2020-04-02 2022-10-27 Takeda Pharmaceutical Company Limited ADAMTS13 variant, compositions, and uses thereof
EP4146686A2 (en) 2020-05-08 2023-03-15 Regeneron Pharmaceuticals, Inc. Vegf traps and mini-traps and methods for treating ocular disorders and cancer
EP4153215A1 (en) 2020-05-22 2023-03-29 Takeda Pharmaceutical Company Limited Adamts13 compositions and methods for treating and diagnosing complications of coronavirus disease
US20230020297A1 (en) * 2020-06-05 2023-01-19 Proabtech Co., Ltd. Uox-albumin conjugate with certain numbers of albumin conjugated thereto, and manufacturing method thereof
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5250421A (en) 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
CZ280901B6 (cs) 1988-10-06 1996-05-15 Hideaki Yamada Způsob kultivace bakterií
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
EP2290053A1 (en) 1994-11-10 2011-03-02 Baxter Healthcare S.A. Method for producing Influenza virus in protein-free cell culture medium
EP0811056B1 (en) 1995-02-23 2000-05-31 Quest International B.V. Peptides for tissue and cell culture media
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
EP2243827B2 (en) 1996-08-30 2017-11-22 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
IT1285158B1 (it) * 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
AU735469B2 (en) 1996-10-07 2001-07-12 Ascend Performance Materials Llc Process for the hydroxylation of benzene
US6103529A (en) 1996-10-10 2000-08-15 Life Technologies, Inc. Animal cell culture media comprising peptides derived from rice
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US6926894B2 (en) 2000-11-22 2005-08-09 Baxter Aktiengesellschaft Composition exhibiting a von willebrand factor (vWF) protease activity comprising a polypeptide chain with the amino acid sequence AAGGILHLELLV
JP4723862B2 (ja) 2002-12-23 2011-07-13 フジフィルム マニュファクチャリング ユーロプ ビー.ブイ. 細胞培養支持体を被覆する方法
EP1737953A2 (en) * 2004-04-16 2007-01-03 Wyeth a Corporation of the State of Delaware Adamts-8 proteins and uses thereof
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
JP2007174948A (ja) * 2005-12-27 2007-07-12 Noritsu Koki Co Ltd 酵素水製造装置
JP4880997B2 (ja) * 2005-12-28 2012-02-22 アルフレッサファーマ株式会社 Adamts13の安定化方法
EP3653699A1 (en) * 2006-01-04 2020-05-20 Baxalta Incorporated Oligopeptide-free cell culture media
WO2008077217A2 (en) * 2006-12-22 2008-07-03 Springer Carrier Ltda. Fresh air command lever for an air conditioning unit
US20090067805A1 (en) 2007-09-10 2009-03-12 Victor Company Of Japan, Limited Recording and reproducing device
US9359629B2 (en) 2007-12-27 2016-06-07 Baxalta Incorporated Cell culture processes
CA2769361A1 (en) * 2009-07-31 2011-02-03 Baxter International Inc. Cell culture medium for adamts protein expression

Also Published As

Publication number Publication date
KR101808496B1 (ko) 2017-12-12
US11254921B2 (en) 2022-02-22
KR20120046301A (ko) 2012-05-09
KR20150044981A (ko) 2015-04-27
AU2010278751B2 (en) 2014-05-15
US9127265B2 (en) 2015-09-08
JP5451884B2 (ja) 2014-03-26
CA2769361A1 (en) 2011-02-03
AU2010278751A1 (en) 2012-02-09
CO6501191A2 (es) 2012-08-15
PL2459702T3 (pl) 2016-12-30
JP6567707B2 (ja) 2019-08-28
EA023193B1 (ru) 2016-05-31
CN104962539B (zh) 2019-09-17
CN104962539A (zh) 2015-10-07
HRP20160783T1 (hr) 2016-08-12
JP6883071B2 (ja) 2021-06-09
WO2011014838A1 (en) 2011-02-03
PT2459702T (pt) 2016-08-02
KR101573972B1 (ko) 2015-12-02
CN102549145B (zh) 2015-05-20
JP2014061005A (ja) 2014-04-10
US20110086413A1 (en) 2011-04-14
KR20150138425A (ko) 2015-12-09
US20170051270A1 (en) 2017-02-23
US10072254B2 (en) 2018-09-11
JP2019201661A (ja) 2019-11-28
HK1171790A1 (zh) 2013-04-05
ES2583258T3 (es) 2016-09-20
KR101912552B1 (ko) 2018-10-26
JP5936232B2 (ja) 2016-06-22
MX2012001261A (es) 2012-05-22
EP2459702A1 (en) 2012-06-06
MX344970B (es) 2017-01-12
US20130071906A1 (en) 2013-03-21
SG178194A1 (en) 2012-03-29
JP2018093881A (ja) 2018-06-21
EA201270224A1 (ru) 2012-09-28
JP6511007B2 (ja) 2019-05-08
BR112012002151A2 (pt) 2020-03-03
CN102549145A (zh) 2012-07-04
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
US20190071660A1 (en) 2019-03-07
US20200308566A1 (en) 2020-10-01
JP2021112210A (ja) 2021-08-05
KR101574056B1 (ko) 2015-12-02
KR20170140411A (ko) 2017-12-20
KR20170081747A (ko) 2017-07-12
HUE029110T2 (en) 2017-02-28
EP2459702B1 (en) 2016-05-04
US20150329848A1 (en) 2015-11-19
US8759026B2 (en) 2014-06-24
BR122021005965B1 (pt) 2022-01-25
BR112012002151B1 (pt) 2021-07-06
JP2016144471A (ja) 2016-08-12
JP2013500724A (ja) 2013-01-10
US9441216B2 (en) 2016-09-13
JP7083059B2 (ja) 2022-06-09
KR101756354B1 (ko) 2017-07-26
NZ597727A (en) 2013-08-30
US8313926B2 (en) 2012-11-20
US20140329294A1 (en) 2014-11-06

Similar Documents

Publication Publication Date Title
IN2012DN00801A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
MX2010000348A (es) Cultivo de celulas madre pluripotentes individuales.
PH12020500027A1 (en) Purification of iduronate-2-sulfatase
MX2013003681A (es) Ácidos nucleicos manipulados y métodos de uso de los mismos.
IN2012DN03825A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
SG10201405377XA (en) Compositions and methods for antibodies targeting complement protein c5
WO2010129294A3 (en) Small molecules supporting pluripotent cell growth and methods thereof
EA201200091A1 (ru) Штаммы talaromyces и ферментные композиции
MX336737B (es) Composiciones y metodos que comprenden variantes de proteasa serina.
EP3486319A3 (en) Compositions and methods comprising serine protease variants
WO2010141801A3 (en) Reprogramming t cells and hematophietic cells
MX361862B (es) Composiciones y métodos que comprenden variante de enzima lipolítica.
MX2016001317A (es) Variantes de enzimas.
MX2009005655A (es) Composiciones y usos de un polipeptido alfa-amilasa del bacilo de la especie 195.
GB0917002D0 (en) Improved shigella blebs
MX2012005782A (es) Procedimientos, composiciones y kits de liofilizacion.
WO2012024573A3 (en) Compositions comrpising perivascular stem cells and nell-1 protein
WO2008085962A3 (en) Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
WO2009137778A3 (en) Methods and compositions for genetically engineering clostridia species
PH12013500851A1 (en) Spoonable yogurt preparations containing non-replicating probiotic micro-organisms
ATE486128T1 (de) Zusammensetzungen und verfahren zur erhöhung der produktion rekombinanter gamma-carboxylierter proteine
WO2009058451A3 (en) Neuronal progenitor cells and methods of derivation and purification of neuronal progenitor cells from embryonic stem cells
GB2500552A (en) Stable lactase drops
MX2009008903A (es) Composiciones de limpieza que comprenden alfa-galactosidasa.
EP1885867A4 (en) NEW PROCEDURES