JP5451884B2 - Ａｄａｍｔｓタンパク質発現のための細胞培養培地 - Google Patents
Ａｄａｍｔｓタンパク質発現のための細胞培養培地 Download PDFInfo
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- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6489—Metalloendopeptidases (3.4.24)
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- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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| US23047709P | 2009-07-31 | 2009-07-31 | |
| US61/230,477 | 2009-07-31 | ||
| PCT/US2010/044020 WO2011014838A1 (en) | 2009-07-31 | 2010-07-30 | Cell culture medium for adamts protein expression |
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| JP2016093240A Active JP6511007B2 (ja) | 2009-07-31 | 2016-05-06 | Ａｄａｍｔｓタンパク質発現のための細胞培養培地 |
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| CA2769361A1 (en) * | 2009-07-31 | 2011-02-03 | Baxter International Inc. | Cell culture medium for adamts protein expression |
| DK3064508T4 (da) | 2010-07-08 | 2023-04-11 | Takeda Pharmaceuticals Co | Fremgangsmåde til fremstilling af rekombinant vwf med høj molekylevægt i cellekultur |
| KR101857380B1 (ko) | 2011-07-01 | 2018-05-11 | 암젠 인크 | 포유동물 세포 배양 |
| EP2906683B1 (en) | 2012-10-15 | 2017-05-31 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
| US9611455B2 (en) | 2013-03-13 | 2017-04-04 | Merck Sharp & Dohme Corp. | Adapted lepidopteran insect cells for the production of recombinant proteins |
| US9217168B2 (en) | 2013-03-14 | 2015-12-22 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
| AR095196A1 (es) * | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
| CA2926517C (en) * | 2013-10-14 | 2021-03-23 | Ares Trading S.A. | New medium for high performance mammalian fed-batch cultures |
| ES2709994T3 (es) | 2014-06-04 | 2019-04-22 | Amgen Inc | Métodos de recolección en cultivos de células de mamífero |
| KR101867134B1 (ko) * | 2015-03-23 | 2018-06-12 | 한양대학교 산학협력단 | 포유류 세포를 이용하여 목적 물질을 고효율로 생산하기 위한 세포 배양 배지, 이를 이용한 세포 배양 방법 및 목적 물질의 생산 방법 |
| MX2017014595A (es) * | 2015-05-22 | 2018-03-21 | Dupont Nutrition Biosci Aps | Acetolactato-descarboxilasa. |
| TWI870789B (zh) | 2015-08-04 | 2025-01-21 | 美商再生元醫藥公司 | 補充牛磺酸之細胞培養基及用法 |
| KR102537083B1 (ko) | 2016-08-04 | 2023-05-25 | 다케다 파머수티컬 컴패니 리미티드 | 겸상적혈구병, 급성 폐 손상 및/또는 급성 호흡 곤란 증후군에서 혈관폐색 위기의 치료, 개선, 및/또는 예방을 위한 adamts13의 용도 |
| AU2017391164B2 (en) * | 2016-11-04 | 2024-01-04 | Takeda Pharmaceutical Company Limited | Adeno-associated virus purification methods |
| SG11201912548XA (en) * | 2017-07-06 | 2020-01-30 | Regeneron Pharma | Cell culture process for making a glycoprotein |
| CA3094076A1 (en) * | 2018-03-16 | 2019-09-19 | Genzyme Corporation | Methods for improving cell viability in a production bioreactor |
| CA3103443A1 (en) * | 2018-06-18 | 2019-12-26 | Synthetic Biologics, Inc. | Methods of making alkaline phosphatase agents |
| WO2021067668A1 (en) | 2019-10-04 | 2021-04-08 | The Children's Medical Center Corporation | Adamts13 treatment to enhance graft survival |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
| TW202128991A (zh) | 2019-12-06 | 2021-08-01 | 美商再生元醫藥公司 | 抗ｖｅｇｆ蛋白組成物及其製備方法 |
| AU2021248679A1 (en) | 2020-04-02 | 2022-10-27 | Takeda Pharmaceutical Company Limited | ADAMTS13 variant, compositions, and uses thereof |
| EP4146686A2 (en) | 2020-05-08 | 2023-03-15 | Regeneron Pharmaceuticals, Inc. | Vegf traps and mini-traps and methods for treating ocular disorders and cancer |
| EP4153215A1 (en) | 2020-05-22 | 2023-03-29 | Takeda Pharmaceutical Company Limited | Adamts13 compositions and methods for treating and diagnosing complications of coronavirus disease |
| US20230020297A1 (en) * | 2020-06-05 | 2023-01-19 | Proabtech Co., Ltd. | Uox-albumin conjugate with certain numbers of albumin conjugated thereto, and manufacturing method thereof |
| CN111808822B (zh) * | 2020-07-02 | 2024-01-12 | 北京百普赛斯生物科技股份有限公司 | 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法 |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4797368A (en) | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
| US5139941A (en) | 1985-10-31 | 1992-08-18 | University Of Florida Research Foundation, Inc. | AAV transduction vectors |
| US5250421A (en) | 1986-01-03 | 1993-10-05 | Genetics Institute, Inc. | Method for producing factor VIII:C-type proteins |
| CZ280901B6 (cs) | 1988-10-06 | 1996-05-15 | Hideaki Yamada | Způsob kultivace bakterií |
| JPH0753104B2 (ja) * | 1989-02-28 | 1995-06-07 | 秀明 山田 | 細菌の培養法 |
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| EP2290053A1 (en) | 1994-11-10 | 2011-03-02 | Baxter Healthcare S.A. | Method for producing Influenza virus in protein-free cell culture medium |
| EP0811056B1 (en) | 1995-02-23 | 2000-05-31 | Quest International B.V. | Peptides for tissue and cell culture media |
| US6013516A (en) | 1995-10-06 | 2000-01-11 | The Salk Institute For Biological Studies | Vector and method of use for nucleic acid delivery to non-dividing cells |
| EP2243827B2 (en) | 1996-08-30 | 2017-11-22 | Life Technologies Corporation | Serum-free mammalian cell culture medium, and uses thereof |
| IT1285158B1 (it) * | 1996-09-17 | 1998-06-03 | Angeletti P Ist Richerche Bio | Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il |
| AU735469B2 (en) | 1996-10-07 | 2001-07-12 | Ascend Performance Materials Llc | Process for the hydroxylation of benzene |
| US6103529A (en) | 1996-10-10 | 2000-08-15 | Life Technologies, Inc. | Animal cell culture media comprising peptides derived from rice |
| US5804420A (en) | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
| US6475725B1 (en) | 1997-06-20 | 2002-11-05 | Baxter Aktiengesellschaft | Recombinant cell clones having increased stability and methods of making and using the same |
| AT407255B (de) | 1997-06-20 | 2001-02-26 | Immuno Ag | Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons |
| US5994136A (en) | 1997-12-12 | 1999-11-30 | Cell Genesys, Inc. | Method and means for producing high titer, safe, recombinant lentivirus vectors |
| US6406909B1 (en) | 1998-07-10 | 2002-06-18 | Chugai Seiyaku Kabushiki Kaisha | Serum-free medium for culturing animal cells |
| AT409379B (de) | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| US6926894B2 (en) | 2000-11-22 | 2005-08-09 | Baxter Aktiengesellschaft | Composition exhibiting a von willebrand factor (vWF) protease activity comprising a polypeptide chain with the amino acid sequence AAGGILHLELLV |
| JP4723862B2 (ja) | 2002-12-23 | 2011-07-13 | フジフィルム マニュファクチャリング ユーロプ ビー．ブイ． | 細胞培養支持体を被覆する方法 |
| EP1737953A2 (en) * | 2004-04-16 | 2007-01-03 | Wyeth a Corporation of the State of Delaware | Adamts-8 proteins and uses thereof |
| US20060094104A1 (en) | 2004-10-29 | 2006-05-04 | Leopold Grillberger | Animal protein-free media for cultivation of cells |
| JP2007174948A (ja) * | 2005-12-27 | 2007-07-12 | Noritsu Koki Co Ltd | 酵素水製造装置 |
| JP4880997B2 (ja) * | 2005-12-28 | 2012-02-22 | アルフレッサファーマ株式会社 | Ａｄａｍｔｓ１３の安定化方法 |
| EP3653699A1 (en) * | 2006-01-04 | 2020-05-20 | Baxalta Incorporated | Oligopeptide-free cell culture media |
| WO2008077217A2 (en) * | 2006-12-22 | 2008-07-03 | Springer Carrier Ltda. | Fresh air command lever for an air conditioning unit |
| US20090067805A1 (en) | 2007-09-10 | 2009-03-12 | Victor Company Of Japan, Limited | Recording and reproducing device |
| US9359629B2 (en) | 2007-12-27 | 2016-06-07 | Baxalta Incorporated | Cell culture processes |
| CA2769361A1 (en) * | 2009-07-31 | 2011-02-03 | Baxter International Inc. | Cell culture medium for adamts protein expression |
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