KR100356140B1 - 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 - Google Patents
인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 Download PDFInfo
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- KR100356140B1 KR100356140B1 KR1019990027418A KR19990027418A KR100356140B1 KR 100356140 B1 KR100356140 B1 KR 100356140B1 KR 1019990027418 A KR1019990027418 A KR 1019990027418A KR 19990027418 A KR19990027418 A KR 19990027418A KR 100356140 B1 KR100356140 B1 KR 100356140B1
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- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 102000054767 gene variant Human genes 0.000 description 1
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000003914 myeloid leukocyte Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- -1 nucleotide triphosphates Chemical class 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 150000003587 threonine derivatives Chemical class 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/02—Antineoplastic agents specific for leukemia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (18)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019990027418A KR100356140B1 (ko) | 1999-07-08 | 1999-07-08 | 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 |
| BRPI0012265A BRPI0012265B8 (pt) | 1999-07-08 | 2000-07-07 | fator estimulante de colônia de granulócitos humano modificado e processo para produzir o mesmo |
| DE60021188T DE60021188T3 (de) | 1999-07-08 | 2000-07-07 | Modifizierter humaner granulozyten-kolonie stimulierenderfaktor sowie verfahren zur herstellung desselben |
| EP00942494A EP1194575B2 (en) | 1999-07-08 | 2000-07-07 | Modified human granulocyte-colony stimulating factor and process for producing same |
| AU57106/00A AU757147C (en) | 1999-07-08 | 2000-07-07 | Modified human granulocyte-colony stimulating factor and process for producing same |
| RU2002103159/13A RU2232772C2 (ru) | 1999-07-08 | 2000-07-07 | Модифицированный колониестимулирующий фактор гранулоцитов человека и способ его получения, днк, экспрессирующий вектор |
| DK00942494T DK1194575T4 (da) | 1999-07-08 | 2000-07-07 | Modificeret human granulocytkolonistimulerende faktor og fremgangsmåde til fremstilling heraf |
| CA2378543A CA2378543C (en) | 1999-07-08 | 2000-07-07 | Modified human granulocyte-colony stimulating factor and process for producing same |
| AT00942494T ATE299187T1 (de) | 1999-07-08 | 2000-07-07 | Modifizierter humaner granulozyten-kolonie stimulierenderfaktor sowie verfahren zur herstellung desselben |
| BR0012265-3A BR0012265A (pt) | 1999-07-08 | 2000-07-07 | Fator estimulante de colÈnia de granulócitos humano modificado e processo para produzir o mesmo |
| PCT/KR2000/000733 WO2001004329A1 (en) | 1999-07-08 | 2000-07-07 | Modified human granulocyte-colony stimulating factor and process for producing same |
| PT00942494T PT1194575E (pt) | 1999-07-08 | 2000-07-07 | Factor de estimulacao de colonias de granulocitos humanos modificados e processos para a sua producao |
| NZ516476A NZ516476A (en) | 1999-07-08 | 2000-07-07 | A hG-CSF in which at least one of the 1st, 2nd, 3rd and 17th amino acids is different from those of wild-type hG-CSF |
| CNB008100942A CN1195859C (zh) | 1999-07-08 | 2000-07-07 | 修饰化人源粒细胞－集落刺激因子及其制备方法 |
| ES00942494T ES2243275T5 (es) | 1999-07-08 | 2000-07-07 | Factor estimulante de colonias de granulocitos humano modificado y un proceso para producir el mismo. |
| JP2001509533A JP2003504069A (ja) | 1999-07-08 | 2000-07-07 | ヒト顆粒球コロニー刺激因子修飾体およびその生産方法 |
| US10/031,123 US20040224393A1 (en) | 1999-07-08 | 2002-01-09 | Modified human granulocyte-colony stimulating factor and process for producing same |
| US11/975,541 US7704709B2 (en) | 1999-07-08 | 2007-10-19 | Modified human granulocyte-colony stimulating factor and process for producing same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019990027418A KR100356140B1 (ko) | 1999-07-08 | 1999-07-08 | 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20010009171A KR20010009171A (ko) | 2001-02-05 |
| KR100356140B1 true KR100356140B1 (ko) | 2002-10-19 |
Family
ID=36129291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019990027418A Expired - Lifetime KR100356140B1 (ko) | 1999-07-08 | 1999-07-08 | 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US20040224393A1 (enExample) |
| EP (1) | EP1194575B2 (enExample) |
| JP (1) | JP2003504069A (enExample) |
| KR (1) | KR100356140B1 (enExample) |
| CN (1) | CN1195859C (enExample) |
| AT (1) | ATE299187T1 (enExample) |
| AU (1) | AU757147C (enExample) |
| BR (2) | BRPI0012265B8 (enExample) |
| CA (1) | CA2378543C (enExample) |
| DE (1) | DE60021188T3 (enExample) |
| DK (1) | DK1194575T4 (enExample) |
| ES (1) | ES2243275T5 (enExample) |
| NZ (1) | NZ516476A (enExample) |
| PT (1) | PT1194575E (enExample) |
| RU (1) | RU2232772C2 (enExample) |
| WO (1) | WO2001004329A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012057529A2 (en) | 2010-10-29 | 2012-05-03 | Hanmi Holdings Co., Ltd. | Method for purifying human granulocyte-colony stimulating factor from recombinant e. coli |
| US9867777B2 (en) | 2010-01-19 | 2018-01-16 | Hanmi Science Co., Ltd. | Liquid formulations for long-acting G-CSF conjugate |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6831158B2 (en) | 2000-01-10 | 2004-12-14 | Maxygen Holdings Ltd. | G-CSF conjugates |
| IL159524A0 (en) | 2001-07-11 | 2004-06-01 | Maxygen Holdings Ltd | G-csf conjugates |
| US7611700B2 (en) * | 2002-09-09 | 2009-11-03 | Hanall Pharmaceuticals, Co., Ltd. | Protease resistant modified interferon alpha polypeptides |
| US8338373B2 (en) * | 2003-10-24 | 2012-12-25 | Nora Therapeutics, Inc. | Method for reducing the risk of spontaneous abortion in a human female subject |
| EP2198875A1 (en) | 2003-10-24 | 2010-06-23 | Nora, LLC | A method for reducing the likelihood of preterm labour in a subject in need thereof |
| US20090226397A1 (en) | 2003-10-24 | 2009-09-10 | Nora Therapeutics, Inc. | Compositions and methods for reducing the likelihood of implantation failure or miscarriage in recipients of artificial insemination |
| AU2006254543A1 (en) | 2005-06-01 | 2006-12-07 | Maxygen Holdings Ltd. | PEGylated G-CSF polypeptides and methods of producing same |
| DE102005033250A1 (de) | 2005-07-15 | 2007-01-18 | Bioceuticals Arzneimittel Ag | Verfahren zur Reinigung von G-CSF |
| EP2274325A4 (en) * | 2008-05-13 | 2011-05-25 | Nora Therapeutics Inc | ANALOGUE OF HUMAN G-CSF AND METHOD OF MANUFACTURE AND APPLICATION THEREOF |
| TWI600759B (zh) | 2010-04-02 | 2017-10-01 | 可娜公司 | 藉由抑制群落刺激因子３（ｃｓｆ３）之天然反義轉錄物以治療ｃｓｆ３相關疾病 |
| RU2467764C2 (ru) * | 2011-01-12 | 2012-11-27 | Государственное бюджетное образовательное учреждение дополнительного профессионального образования "Пензенский институт усовершенствования врачей" Министерства здравоохранения и социального развития Российской Федерации | Способ лечения больных аутоиммунной хронической крапивницей с использованием внутривенного введения препарата "габриглобин" |
| KR101623906B1 (ko) | 2014-07-23 | 2016-05-24 | 주식회사 이큐스앤자루 | 과립구 콜로니 자극인자 변이 단백질 또는 이의 트랜스페린 융합 단백질을 유효성분으로 포함하는 약학적 조성물 |
| WO2016013725A1 (ko) * | 2014-07-23 | 2016-01-28 | 주식회사 이큐스앤자루 | 과립구 콜로니 자극인자 변이 단백질 또는 이의 트랜스페린 융합 단백질을 유효성분으로 포함하는 약학적 조성물 |
| KR102645064B1 (ko) | 2017-02-03 | 2024-03-08 | 한미약품 주식회사 | 지속성이 증가된 생리활성 물질의 결합체 및 이의 용도 |
| US11267858B2 (en) | 2019-05-31 | 2022-03-08 | Spectrum Pharmaceuticals, Inc. | Methods of treatment using G-CSF protein complex |
| US11684655B2 (en) | 2019-05-31 | 2023-06-27 | Spectrum Pharmaceuticals, Inc. | Methods of treating neutorpenia using G-CSF protein complex |
| KR102375269B1 (ko) * | 2021-01-27 | 2022-03-17 | 한미약품 주식회사 | 단백질 액상 제제 및 이의 제조방법 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4810643A (en) * | 1985-08-23 | 1989-03-07 | Kirin- Amgen Inc. | Production of pluripotent granulocyte colony-stimulating factor |
| JPH01500483A (ja) * | 1986-08-11 | 1989-02-23 | シタス コーポレイション | Ｇ‐ｃｓｆ及びそのミューテインの発現 |
| US5362853A (en) * | 1986-12-23 | 1994-11-08 | Kyowa Hakko Kogyo Co., Ltd. | Polypeptide derivatives of human granulocyte colony stimulating factor |
| US5714581A (en) * | 1986-12-23 | 1998-02-03 | Kyowa Hakko Kogyo Co., Ltd. | Polypeptide derivatives of human granulocyte colony stimulating factor |
| JPH02104598A (ja) | 1988-04-12 | 1990-04-17 | Kirin Amgen Inc | ヒト顆粒球コロニー刺激因子ポリペプチド誘導体 |
| NZ236819A (en) | 1990-02-03 | 1993-07-27 | Max Planck Gesellschaft | Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions |
| GB9107846D0 (en) | 1990-04-30 | 1991-05-29 | Ici Plc | Polypeptides |
| EP0626448A3 (de) | 1993-05-26 | 1998-01-14 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verfahren zur Herstellung und Reinigung von alpha-Interferon |
| US6476198B1 (en) | 1993-07-13 | 2002-11-05 | The Scripps Research Institute | Multispecific and multivalent antigen-binding polypeptide molecules |
| US5451660A (en) * | 1993-12-13 | 1995-09-19 | Genentech, Inc. | Method for purifying polypeptides |
| EP0789776B1 (en) | 1994-11-01 | 2001-06-13 | Winfried Wels | Nucleic acid transfer system |
| US6100070A (en) | 1995-10-05 | 2000-08-08 | G. D. Searle & Co. | G-CSF receptor agonists |
| KR100316347B1 (ko) * | 1998-09-15 | 2002-08-27 | 한미약품(주) | 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 |
| NZ522847A (en) * | 2000-05-16 | 2004-11-26 | Bolder Biotechnology Inc | Methods for refolding proteins containing free cysteine residues |
-
1999
- 1999-07-08 KR KR1019990027418A patent/KR100356140B1/ko not_active Expired - Lifetime
-
2000
- 2000-07-07 DE DE60021188T patent/DE60021188T3/de not_active Expired - Lifetime
- 2000-07-07 RU RU2002103159/13A patent/RU2232772C2/ru active
- 2000-07-07 BR BRPI0012265A patent/BRPI0012265B8/pt unknown
- 2000-07-07 CN CNB008100942A patent/CN1195859C/zh not_active Expired - Lifetime
- 2000-07-07 EP EP00942494A patent/EP1194575B2/en not_active Expired - Lifetime
- 2000-07-07 DK DK00942494T patent/DK1194575T4/da active
- 2000-07-07 PT PT00942494T patent/PT1194575E/pt unknown
- 2000-07-07 CA CA2378543A patent/CA2378543C/en not_active Expired - Lifetime
- 2000-07-07 BR BR0012265-3A patent/BR0012265A/pt not_active IP Right Cessation
- 2000-07-07 ES ES00942494T patent/ES2243275T5/es not_active Expired - Lifetime
- 2000-07-07 NZ NZ516476A patent/NZ516476A/en not_active IP Right Cessation
- 2000-07-07 JP JP2001509533A patent/JP2003504069A/ja active Pending
- 2000-07-07 AU AU57106/00A patent/AU757147C/en not_active Expired
- 2000-07-07 WO PCT/KR2000/000733 patent/WO2001004329A1/en not_active Ceased
- 2000-07-07 AT AT00942494T patent/ATE299187T1/de active
-
2002
- 2002-01-09 US US10/031,123 patent/US20040224393A1/en not_active Abandoned
-
2007
- 2007-10-19 US US11/975,541 patent/US7704709B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9867777B2 (en) | 2010-01-19 | 2018-01-16 | Hanmi Science Co., Ltd. | Liquid formulations for long-acting G-CSF conjugate |
| WO2012057529A2 (en) | 2010-10-29 | 2012-05-03 | Hanmi Holdings Co., Ltd. | Method for purifying human granulocyte-colony stimulating factor from recombinant e. coli |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2232772C2 (ru) | 2004-07-20 |
| WO2001004329A1 (en) | 2001-01-18 |
| JP2003504069A (ja) | 2003-02-04 |
| ATE299187T1 (de) | 2005-07-15 |
| BRPI0012265B8 (pt) | 2021-05-25 |
| EP1194575A4 (en) | 2002-10-24 |
| DE60021188D1 (de) | 2005-08-11 |
| CN1195859C (zh) | 2005-04-06 |
| ES2243275T3 (es) | 2005-12-01 |
| KR20010009171A (ko) | 2001-02-05 |
| AU5710600A (en) | 2001-01-30 |
| CA2378543A1 (en) | 2001-01-18 |
| EP1194575A1 (en) | 2002-04-10 |
| BR0012265A (pt) | 2002-03-12 |
| ES2243275T5 (es) | 2009-12-02 |
| DK1194575T3 (da) | 2005-10-17 |
| AU757147C (en) | 2005-03-03 |
| EP1194575B1 (en) | 2005-07-06 |
| BRPI0012265B1 (pt) | 2018-11-21 |
| US20040224393A1 (en) | 2004-11-11 |
| PT1194575E (pt) | 2005-10-31 |
| NZ516476A (en) | 2003-09-26 |
| US7704709B2 (en) | 2010-04-27 |
| EP1194575B2 (en) | 2009-08-26 |
| DE60021188T2 (de) | 2006-04-27 |
| US20080064066A1 (en) | 2008-03-13 |
| DK1194575T4 (da) | 2009-12-07 |
| DE60021188T3 (de) | 2010-04-01 |
| CN1360636A (zh) | 2002-07-24 |
| CA2378543C (en) | 2010-05-18 |
| AU757147B2 (en) | 2003-02-06 |
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