JPH05507209A - チオレドキシンおよびチオレドキシン様分子に対するペプチドおよび蛋白融合 - Google Patents
チオレドキシンおよびチオレドキシン様分子に対するペプチドおよび蛋白融合Info
- Publication number
- JPH05507209A JPH05507209A JP92507259A JP50725992A JPH05507209A JP H05507209 A JPH05507209 A JP H05507209A JP 92507259 A JP92507259 A JP 92507259A JP 50725992 A JP50725992 A JP 50725992A JP H05507209 A JPH05507209 A JP H05507209A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- thioredoxin
- fusion
- dna sequence
- proteins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5403—IL-3
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5412—IL-6
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5431—IL-11
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (11)
- (1)選択した異種蛋白をコード化するDNA配列に融合したチオレドキシン様 蛋白をコード化するDNAを含む、融合蛋白をコード化するDNA配列。
- (2)チオレドキシン様蛋白をコード化するDNA配列が融合蛋白のアミノ末端 を含む、請求項1記載のDNA配列。
- (3)チオレドキシン様蛋白をコード化するDNA配列が融合蛋白のカルボキシ 末端を含む、請求項1記載のDNA配列。
- (4)チオレドキシン様蛋白をコード化するDNA配列がエシエリキア・コリ( EColi)チオレドキシンおよびひとチオレドキシンからなる群から選ばれる 、請求項1、2または3記載のDNA配列。
- (5)選択した蛋白をコード化するDNA配列がIL−11、IL−6、マクロ ファージ阻害蛋白1αおよび骨形態形成蛋白2からなる群から選ばれる、請求項 1、2または3記載のDNA配列。
- (6)さらにチオレドキシン様蛋白をコード化するDNAと選択した異種蛋白を コード化するDNAの間に融合したリンカーDNA配列を含む、請求項1、2ま たは3記載のDNA配列。
- (7)配列が、選択した宿主細胞中における融合蛋白の発現を指示する能力をも つ適当な発現制御配列の制御下にある、請求項1−6のDNA配列を含むプラス ミドDNA分子。
- (8)請求項7記載のプラスミドで形質転換されるか、またはそれをそのゲノム 中へ組込まれた、エシエリキア・コリ(E.Coli)宿主細胞。
- (9)(a)適当な条件下、培地中で請求項8記載の宿主細胞を培養し、(b) それにより産された融合蛋白を上記培地から採取し、(c)上記融合蛋白から選 択した異種蛋白を切断し、(d)選択した異種蛋白を分離することを含む選択し た異種蛋白の製造法。
- (10)請求項9記載の方法で製造されるIL−11蛋白。
- (11)請求項9記載の方法におけるチオレドキシンの使用。
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US65253191A | 1991-02-06 | 1991-02-06 | |
US745.382 | 1991-08-14 | ||
US07/745,382 US5270181A (en) | 1991-02-06 | 1991-08-14 | Peptide and protein fusions to thioredoxin and thioredoxin-like molecules |
US652.531 | 1991-08-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH05507209A true JPH05507209A (ja) | 1993-10-21 |
JP2513978B2 JP2513978B2 (ja) | 1996-07-10 |
Family
ID=27096313
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4507259A Expired - Lifetime JP2513978B2 (ja) | 1991-02-06 | 1992-02-06 | チオレドキシンおよびチオレドキシン様分子に対するペプチドおよび蛋白融合 |
Country Status (9)
Country | Link |
---|---|
US (1) | US5270181A (ja) |
EP (3) | EP1231217B1 (ja) |
JP (1) | JP2513978B2 (ja) |
AT (2) | ATE237692T1 (ja) |
AU (1) | AU663489B2 (ja) |
CA (1) | CA2093643C (ja) |
DE (2) | DE69233779D1 (ja) |
MX (1) | MX9203295A (ja) |
WO (1) | WO1992013955A1 (ja) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0781848A2 (en) | 1995-12-28 | 1997-07-02 | FUJIREBIO Inc. | Fused DNA sequence, fused protein expressed from said fused DNA sequence and method for expressing said fused protein |
WO2004087917A1 (ja) * | 2003-03-31 | 2004-10-14 | National Institute Of Advanced Industrial Science And Technology | チオレドキシン改変体 |
US7259242B2 (en) | 1997-01-08 | 2007-08-21 | Invitrogen Corporation | Methods for production of proteins |
JP2009531294A (ja) * | 2006-02-24 | 2009-09-03 | シエシー ファルマセウティチィ ソシエタ ペル アチオニ | 抗アミロイド免疫原性組成物、方法、および使用 |
JP2012513453A (ja) * | 2008-12-23 | 2012-06-14 | アイエスピー・インヴェストメンツ・インコーポレイテッド | Hmg−coa還元酵素由来のペプチド、および、前記ペプチドを含む美容または医薬組成物 |
Families Citing this family (105)
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US5646016A (en) * | 1991-02-06 | 1997-07-08 | Genetics Institute, Inc. | Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules |
CA2119463C (en) | 1991-09-20 | 2003-09-16 | Leu-Fen H. Lin | Glial cell line-derived neutrophic factor |
EP0670730B1 (en) * | 1992-03-30 | 2003-06-04 | Immunex Corporation | Fusion protein comprising two tumor necrosis factor receptors |
IL105406A (en) | 1992-04-17 | 2000-01-31 | Sankyo Co | Derivatives of adipogenesis inhibitory factor processes for the preparation thereof and pharmaceutical compositions containing the same |
CA2153254C (en) * | 1993-01-15 | 2002-05-21 | Edward R. Lavallie | Cloning of enterokinase and method of use |
US6746859B1 (en) * | 1993-01-15 | 2004-06-08 | Genetics Institute, Llc | Cloning of enterokinase and method of use |
US5563046A (en) * | 1993-08-02 | 1996-10-08 | Celtrix Pharmaceuticals, Inc. | Fusion polypeptides and proteins |
US5914254A (en) * | 1993-08-02 | 1999-06-22 | Celtrix Pharmaceuticals, Inc. | Expression of fusion polypeptides transported out of the cytoplasm without leader sequences |
US6270757B1 (en) | 1994-04-21 | 2001-08-07 | Genetics Institute, Inc. | Formulations for IL-11 |
US5635182A (en) * | 1994-06-16 | 1997-06-03 | Genetics Institute, Inc. | Method of detecting ligand interactions |
US6399296B1 (en) * | 1994-07-20 | 2002-06-04 | The General Hospital Corporation | Interaction trap systems for detecting protein interactions |
WO1996002561A1 (en) * | 1994-07-20 | 1996-02-01 | The General Hospital Corporation | Interaction trap systems for detecting protein interactions |
US5582821A (en) * | 1994-07-22 | 1996-12-10 | Genetics Institute, Inc. | Methods for treating bleeding disorders |
US5639635A (en) * | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
US5789199A (en) * | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
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EP0781848A2 (en) | 1995-12-28 | 1997-07-02 | FUJIREBIO Inc. | Fused DNA sequence, fused protein expressed from said fused DNA sequence and method for expressing said fused protein |
US7259242B2 (en) | 1997-01-08 | 2007-08-21 | Invitrogen Corporation | Methods for production of proteins |
US7265206B2 (en) | 1997-01-08 | 2007-09-04 | Invitrogen Corporation | Methods of production of proteins |
WO2004087917A1 (ja) * | 2003-03-31 | 2004-10-14 | National Institute Of Advanced Industrial Science And Technology | チオレドキシン改変体 |
JP2009531294A (ja) * | 2006-02-24 | 2009-09-03 | シエシー ファルマセウティチィ ソシエタ ペル アチオニ | 抗アミロイド免疫原性組成物、方法、および使用 |
JP2012513453A (ja) * | 2008-12-23 | 2012-06-14 | アイエスピー・インヴェストメンツ・インコーポレイテッド | Hmg−coa還元酵素由来のペプチド、および、前記ペプチドを含む美容または医薬組成物 |
Also Published As
Publication number | Publication date |
---|---|
EP1231217A3 (en) | 2004-01-21 |
DE69233008D1 (de) | 2003-05-22 |
CA2093643C (en) | 2001-10-30 |
EP1231217B1 (en) | 2010-01-27 |
EP1231275A3 (en) | 2004-01-21 |
EP1231275A2 (en) | 2002-08-14 |
MX9203295A (es) | 1992-07-01 |
DE69233779D1 (de) | 2010-03-18 |
ATE237692T1 (de) | 2003-05-15 |
EP0574506A1 (en) | 1993-12-22 |
EP0574506B1 (en) | 2003-04-16 |
EP1231217A2 (en) | 2002-08-14 |
JP2513978B2 (ja) | 1996-07-10 |
CA2093643A1 (en) | 1992-08-07 |
DE69233008T2 (de) | 2004-01-22 |
ATE456661T1 (de) | 2010-02-15 |
AU663489B2 (en) | 1995-10-12 |
WO1992013955A1 (en) | 1992-08-20 |
AU1467192A (en) | 1992-09-07 |
US5270181A (en) | 1993-12-14 |
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