JPWO2012111187A1 - 皮膚コラーゲン産生促進剤 - Google Patents
皮膚コラーゲン産生促進剤 Download PDFInfo
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- JPWO2012111187A1 JPWO2012111187A1 JP2012503560A JP2012503560A JPWO2012111187A1 JP WO2012111187 A1 JPWO2012111187 A1 JP WO2012111187A1 JP 2012503560 A JP2012503560 A JP 2012503560A JP 2012503560 A JP2012503560 A JP 2012503560A JP WO2012111187 A1 JPWO2012111187 A1 JP WO2012111187A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
- A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
- A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
- A61K8/355—Quinones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- Health & Medical Sciences (AREA)
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- Veterinary Medicine (AREA)
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- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Birds (AREA)
- Dermatology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gerontology & Geriatric Medicine (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Pulmonology (AREA)
- Toxicology (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
正常ヒト線維芽細胞を用いて、メナキノン−7の皮膚コラーゲン産生促進作用をビタミンK1およびメナキノン−4と比較した。メナキノン−7試薬(和光純薬工業株式会社)は、HPLCで純度検定したものを使用した。設定濃度は、細胞培養系におけるビタミンK類の最大溶解度1.5x10−5Mで比較した。ビタミンK1(和光純薬工業株式会社)およびメナキノン−4(Sigma社)の純度はHPLC法により検定したものを使用した。
正常ヒト真皮線維芽細胞(クラボウ社製)を0.5重量%仔牛血清(FBS)含有ダルベッコ改変イーグル培地(DMEM)(Sigma社)を用いて1ウェル当たり2.0x104個の細胞密度となるように96穴マイクロプレートに播種した。播種48時間後、表5に記載の濃度のメナキノン−7を含有する0.5重量%FBS含有DMEMと交換した。メナキノン−7は、エタノールに溶解した後、0.5%FBS含有DMEMにて100倍希釈した溶液を試験最高濃度液として調製した。また、陽性コントロールとしてアスコルビン酸リン酸マグネシウム塩(VC−PMg)を用い、試験方法に問題がないことを確認した。48時間試料含有培地で培養した後、培地上清を回収してI型コラーゲン量測定のELISAに供した。細胞は0.5%Triton X−100溶液にて溶解後、タンパク量を定量した。
培地および検量線用I型コラーゲン溶液を高吸着型ELISAプレートの各ウェルに入れ、37℃にて2時間コーティングした後、1%牛血清アルブミン(BSA)溶液を用いて37℃にて1時間ブロッキングした。一次抗体反応は、Anti−Human Collagen Type I antibody (Rabbit)を0.3%BSA溶液で希釈し、37℃にて1時間反応させた。ニ次抗体反応は、ヒストファインMAX−PO(R)(Rabbit)をリン酸緩衝液で希釈し、37℃にて1時間反応させた。次に、0.3mg/mlの2,2’−Azinobis(3−ethylbenzothiazoline−6−sulfonic acid)diammounium salt (ABTS)および0.03%の過酸化水素を含むリン酸−クエン酸緩衝液(0.1M,pH 4.0)溶液を加えて20分間反応させ、マイクロプレートリーダーにて405nmの吸光度を測定した。
表5に示す濃度範囲のメナキノン−7におけるヒト真皮線維芽細胞に対するコラーゲン産生促進作用を調べた。結果を表5に示す。試料添加は各群n=6で試験を実施し、結果はそれぞれの平均値で示している。なお、統計処理については、試料未添加の対照群に対するStudent t検定を実施し、1%未満の危険率の場合*をつけた。
Wistar系ラット(雄、5週齢)を、精製飼料AIN93G(オリエンタル酵母社製)を1週間自由に摂取させ馴化した後、群間の体重が均一になるように1群3匹に群分けした。ラット標準食(AIN93G)と、メナキノン−7を添加したメナキノン−7添加飼料(メナキノン−7:150μg/g)を各群3匹ずつのラットに、90日間自由摂取させた後、皮膚中のビタミンK濃度を測定した。ビタミンKの分析は、高速液体クロマトグラフィーを用い、白金黒を用いたポストカラム還元蛍光検出法により分析した(Sato et al.Biochim.Biophys.Acta 1622,145−150)。結果を表6に示す。
4週齢のCrl:CD(SD)(SPF)雄ラットを、入荷後1週間の検疫期間を含め投与開始まで馴化した。検疫・馴化期間中、一般状態観察を毎日行い、体重を入荷後1日(入荷翌日)、3日及び7日に測定した。群間の体重が均一になるように1群5匹に4群に群わけをした。
Claims (3)
- メナキノン−7を有効成分として含有する皮膚コラーゲン産生促進剤。
- 請求項1に記載の皮膚コラーゲン産生促進剤を含む経皮投与剤。
- 請求項1に記載の皮膚コラーゲン産生促進剤を含む経口投与剤。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012503560A JP5062922B2 (ja) | 2011-02-14 | 2011-08-09 | 皮膚コラーゲン産生促進剤 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011028189 | 2011-02-14 | ||
JP2011028189 | 2011-02-14 | ||
JP2012503560A JP5062922B2 (ja) | 2011-02-14 | 2011-08-09 | 皮膚コラーゲン産生促進剤 |
PCT/JP2011/068123 WO2012111187A1 (ja) | 2011-02-14 | 2011-08-09 | 皮膚コラーゲン産生促進剤 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP5062922B2 JP5062922B2 (ja) | 2012-10-31 |
JPWO2012111187A1 true JPWO2012111187A1 (ja) | 2014-07-03 |
Family
ID=46672141
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012503560A Active JP5062922B2 (ja) | 2011-02-14 | 2011-08-09 | 皮膚コラーゲン産生促進剤 |
Country Status (7)
Country | Link |
---|---|
US (2) | US20130137640A1 (ja) |
EP (1) | EP2561869B1 (ja) |
JP (1) | JP5062922B2 (ja) |
KR (1) | KR101781671B1 (ja) |
CN (1) | CN103347505B (ja) |
BR (1) | BR112012027864A2 (ja) |
WO (1) | WO2012111187A1 (ja) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2878295A4 (en) * | 2012-07-24 | 2015-12-23 | J Oil Mills Inc | COMPOSITION |
CN104188961A (zh) * | 2014-09-17 | 2014-12-10 | 朱忠良 | 一种皮肤胶原蛋白增强剂及其应用 |
EP3250180A2 (en) | 2015-01-27 | 2017-12-06 | VitaK B.V. | Epidermal skin health |
CN110418631A (zh) * | 2017-03-17 | 2019-11-05 | 玫琳凯有限公司 | 化妆品组合物及方法 |
EP3427727B1 (de) * | 2017-07-14 | 2022-12-28 | BioActive Food GmbH | Kombination von biologisch aktiven substanzen zur stabilisierung oder erhöhung des kollagengehalts |
Family Cites Families (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS647541A (en) | 1987-06-30 | 1989-01-11 | Toshiba Corp | Manufacture of semiconductor device |
JPH01135455A (ja) | 1987-11-20 | 1989-05-29 | Sanwa Tekki Corp | タンクの内面バフ研磨装置 |
JPH05320039A (ja) * | 1991-09-20 | 1993-12-03 | Taiyo Kagaku Co Ltd | 美白化粧料 |
JP2900238B2 (ja) * | 1994-07-07 | 1999-06-02 | 株式会社ホーネンコーポレーション | 天然メナキノン−7高含量脂質 |
JPH107541A (ja) | 1996-06-20 | 1998-01-13 | Noevir Co Ltd | 皮膚外用剤 |
JPH1135455A (ja) | 1997-07-18 | 1999-02-09 | Noevir Co Ltd | コラーゲン産生促進剤、及びこれを含有する皮膚の老化防止用又は創傷治癒促進用皮膚外用剤 |
JP2000256176A (ja) * | 1999-03-02 | 2000-09-19 | Shalom:Kk | 皮膚化粧料 |
KR20030089710A (ko) * | 2001-04-06 | 2003-11-22 | 가부시키가이샤 도요학코 | 미용화장품 소재 및 그 제조방법 |
US20040023560A1 (en) * | 2002-08-05 | 2004-02-05 | Swoboda Gary L. | Apparatus and method for a reversible emulator/target cable connector |
JP2004196759A (ja) | 2002-12-18 | 2004-07-15 | Takashi Suzuki | ビタミンk類とビタミンe複合の美肌化粧料 |
US20050123603A1 (en) * | 2003-09-26 | 2005-06-09 | Natural Asa | Natural menaquinone 7 compositions |
BRPI0415229A (pt) * | 2003-10-08 | 2006-12-05 | Otsuka Pharma Co Ltd | composição para promoção de produção de colágeno |
KR101332869B1 (ko) | 2005-04-15 | 2013-11-25 | 알버트 아인슈타인 컬리지 오브 메디신 오브 예쉬바 유니버시티 | 항-egfr 치료법에 속발된 피부 발진의 예방 및 치료를위한 비타민 k |
JP4033877B2 (ja) | 2005-09-29 | 2008-01-16 | 株式会社ファンケル | I型コラーゲン産生促進用組成物 |
JP4688683B2 (ja) | 2006-01-16 | 2011-05-25 | 株式会社資生堂 | I型コラーゲン産生促進剤および抗しわ剤 |
JP5111822B2 (ja) | 2006-10-11 | 2013-01-09 | 東洋ビューティ株式会社 | コラーゲン産生促進剤および抗老化化粧料 |
JP4836923B2 (ja) | 2006-10-31 | 2011-12-14 | 株式会社山田養蜂場本社 | コラーゲンとヒアルロン酸の産生促進剤 |
JP2008189607A (ja) | 2007-02-06 | 2008-08-21 | Tama Seikagaku Kk | I型コラーゲン産生促進剤及び皮膚外用剤 |
JP5224770B2 (ja) | 2007-03-19 | 2013-07-03 | 金印株式会社 | コラーゲン産生促進剤、促進方法、香粧品、飲食品および医薬品 |
JP5537151B2 (ja) * | 2007-04-05 | 2014-07-02 | 株式会社J−オイルミルズ | 精神安定剤および機能性食品 |
JP5184813B2 (ja) | 2007-05-11 | 2013-04-17 | 日本メナード化粧品株式会社 | コラーゲン生成促進及び分解抑制剤 |
CA2694318A1 (en) * | 2007-07-24 | 2009-05-22 | Viridis Biopharma Pvt Ltd. | Treatment of human disease conditions and disorders using vitamin k analogues and derivatives |
JP2009184997A (ja) | 2008-02-08 | 2009-08-20 | B & C Laboratories Inc | コラーゲン産生促進剤 |
US8815953B2 (en) | 2008-03-13 | 2014-08-26 | Spectrum Pharmaceuticals, Inc. | Formulations of vitamin K analogs for topical use |
JP2009249366A (ja) | 2008-04-10 | 2009-10-29 | Kose Corp | コラーゲン産生促進剤、及び老化防止用皮膚外用剤 |
JP5255919B2 (ja) | 2008-06-12 | 2013-08-07 | ヒノキ新薬株式会社 | コラーゲン産生促進剤 |
JP5503130B2 (ja) | 2008-10-20 | 2014-05-28 | ユニチカ株式会社 | コラーゲン産生促進剤 |
JP5320039B2 (ja) | 2008-11-26 | 2013-10-23 | 花王株式会社 | サーマルインクジェット記録用水分散体の製造方法 |
-
2011
- 2011-08-09 JP JP2012503560A patent/JP5062922B2/ja active Active
- 2011-08-09 WO PCT/JP2011/068123 patent/WO2012111187A1/ja active Application Filing
- 2011-08-09 KR KR1020137024186A patent/KR101781671B1/ko active IP Right Grant
- 2011-08-09 BR BR112012027864A patent/BR112012027864A2/pt not_active Application Discontinuation
- 2011-08-09 EP EP11858921.7A patent/EP2561869B1/en not_active Not-in-force
- 2011-08-09 CN CN201180067097.2A patent/CN103347505B/zh not_active Expired - Fee Related
-
2012
- 2012-10-03 US US13/644,210 patent/US20130137640A1/en not_active Abandoned
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2015
- 2015-02-16 US US14/623,243 patent/US9522109B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN103347505B (zh) | 2015-02-04 |
US20150164767A1 (en) | 2015-06-18 |
JP5062922B2 (ja) | 2012-10-31 |
EP2561869A4 (en) | 2013-09-04 |
EP2561869B1 (en) | 2016-12-14 |
US9522109B2 (en) | 2016-12-20 |
WO2012111187A1 (ja) | 2012-08-23 |
CN103347505A (zh) | 2013-10-09 |
US20130137640A1 (en) | 2013-05-30 |
BR112012027864A2 (pt) | 2016-08-09 |
EP2561869A1 (en) | 2013-02-27 |
KR20140012990A (ko) | 2014-02-04 |
KR101781671B1 (ko) | 2017-09-25 |
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