JPS6269988A - デキストラナーゼを安定に固定化したハイドロキシアパタイトの調整法 - Google Patents
デキストラナーゼを安定に固定化したハイドロキシアパタイトの調整法Info
- Publication number
- JPS6269988A JPS6269988A JP60206677A JP20667785A JPS6269988A JP S6269988 A JPS6269988 A JP S6269988A JP 60206677 A JP60206677 A JP 60206677A JP 20667785 A JP20667785 A JP 20667785A JP S6269988 A JPS6269988 A JP S6269988A
- Authority
- JP
- Japan
- Prior art keywords
- dextranase
- hydroxyapatite
- protein
- immobilized
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229910052588 hydroxylapatite Inorganic materials 0.000 title claims abstract description 42
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 title claims abstract description 42
- 238000004519 manufacturing process Methods 0.000 title description 3
- 108010001682 Dextranase Proteins 0.000 claims abstract description 38
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 32
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 32
- 108010014251 Muramidase Proteins 0.000 claims abstract description 5
- 102000016943 Muramidase Human genes 0.000 claims abstract description 5
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 5
- 239000012736 aqueous medium Substances 0.000 claims abstract description 5
- 229960000274 lysozyme Drugs 0.000 claims abstract description 5
- 239000004325 lysozyme Substances 0.000 claims abstract description 5
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 12
- 230000001588 bifunctional effect Effects 0.000 claims description 4
- 230000003100 immobilizing effect Effects 0.000 claims description 3
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 10
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract description 9
- 238000003756 stirring Methods 0.000 abstract description 6
- 108010093096 Immobilized Enzymes Proteins 0.000 abstract description 4
- 230000002255 enzymatic effect Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 150000001299 aldehydes Chemical class 0.000 abstract description 2
- 230000009849 deactivation Effects 0.000 abstract description 2
- 239000000725 suspension Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 27
- 230000000694 effects Effects 0.000 description 16
- 239000000243 solution Substances 0.000 description 8
- 238000001179 sorption measurement Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 229940088598 enzyme Drugs 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 229920002307 Dextran Polymers 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 239000000551 dentifrice Substances 0.000 description 3
- 230000000087 stabilizing effect Effects 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- ULDHMXUKGWMISQ-UHFFFAOYSA-N carvone Chemical compound CC(=C)C1CC=C(C)C(=O)C1 ULDHMXUKGWMISQ-UHFFFAOYSA-N 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000005498 polishing Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000000606 toothpaste Substances 0.000 description 2
- 229940034610 toothpaste Drugs 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 239000005973 Carvone Substances 0.000 description 1
- 102000011632 Caseins Human genes 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 241000257465 Echinoidea Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010058683 Immobilized Proteins Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000003872 anastomosis Effects 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 239000012770 industrial material Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229940041616 menthol Drugs 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 210000003781 tooth socket Anatomy 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/24—Phosphorous; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/18—Multi-enzyme systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Inorganic Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Cosmetics (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60206677A JPS6269988A (ja) | 1985-09-20 | 1985-09-20 | デキストラナーゼを安定に固定化したハイドロキシアパタイトの調整法 |
| US06/878,256 US4812404A (en) | 1985-09-20 | 1986-06-25 | Apatite immobilized glucanase |
| CA000517894A CA1285511C (en) | 1985-09-20 | 1986-09-10 | Apatite immobilized glucanase and the like, and method of preparingthe same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60206677A JPS6269988A (ja) | 1985-09-20 | 1985-09-20 | デキストラナーゼを安定に固定化したハイドロキシアパタイトの調整法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6269988A true JPS6269988A (ja) | 1987-03-31 |
| JPH053275B2 JPH053275B2 (enExample) | 1993-01-14 |
Family
ID=16527286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60206677A Granted JPS6269988A (ja) | 1985-09-20 | 1985-09-20 | デキストラナーゼを安定に固定化したハイドロキシアパタイトの調整法 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US4812404A (enExample) |
| JP (1) | JPS6269988A (enExample) |
| CA (1) | CA1285511C (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2617710A1 (fr) * | 1986-04-22 | 1989-01-13 | Dental Chem Co Ltd | Composition dentifrice |
| JPS6430584A (en) * | 1987-07-25 | 1989-02-01 | Ngk Insulators Ltd | Carrier for immobilizing organism catalyst |
| GB2224727B (en) * | 1988-09-29 | 1992-01-22 | Sangi Kk | Antimicrobial hydroxyapatite powders |
| US5268174A (en) * | 1988-09-29 | 1993-12-07 | Kabushiki Kaisha Sangi | Antimicrobial hydroxyapatite powders containing hinokitiol, protamine or sorbic acid |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0425016B1 (en) * | 1989-10-27 | 1995-12-20 | The Procter & Gamble Company | Antimicrobial method and formulation employing type II endoglycosidase and antimicrobial agent |
| JP2004067547A (ja) * | 2002-08-02 | 2004-03-04 | Yoshinori Kuboki | チタン繊維医療材料 |
| CN100434907C (zh) * | 2006-09-28 | 2008-11-19 | 东北电力大学 | 细胞色素c分子自组装纳米有序复合结构组装体及制备方法 |
| ES2319059B1 (es) * | 2007-09-07 | 2010-02-12 | Universidad Complutense De Madrid | Brushita como sistema de inmovilizacion de enzimas y su uso en biosensores amperometricos biocompatibles. |
| JP4594439B1 (ja) * | 2009-12-29 | 2010-12-08 | 靖雄 知念 | 虫歯治療薬およびその製造方法 |
| CN111154744B (zh) * | 2019-12-24 | 2022-02-01 | 克劳丽化妆品股份有限公司 | 一种羟基磷灰石固定化α葡聚糖酶及其制备方法和应用 |
| CN112111479A (zh) * | 2020-09-30 | 2020-12-22 | 江苏海洋大学 | 一种右旋糖酐酶与羟基磷灰石复合材料及其制备方法和用途 |
| CN116999344A (zh) * | 2023-09-19 | 2023-11-07 | 余昭军 | 一种生物酶组合物及其制备方法及制得的牙膏 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54101409A (en) * | 1977-11-14 | 1979-08-10 | Minnesota Mining & Mfg | Protein fixing agent |
| JPS56134989A (en) * | 1980-02-26 | 1981-10-22 | Tate & Lyle Ltd | Immobilized enzyme |
| JPS578785A (en) * | 1980-06-16 | 1982-01-18 | Nitto Electric Ind Co Ltd | Preparation of immobilized enzyme |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4464468A (en) * | 1968-03-29 | 1984-08-07 | Agence Nationale De Valorisation De La Recherche (Anvar) | Immobilization of active protein by cross-linking to inactive protein |
| US4155546A (en) * | 1977-07-27 | 1979-05-22 | Itek Corporation | Bottom sheet feeding apparatus |
-
1985
- 1985-09-20 JP JP60206677A patent/JPS6269988A/ja active Granted
-
1986
- 1986-06-25 US US06/878,256 patent/US4812404A/en not_active Expired - Fee Related
- 1986-09-10 CA CA000517894A patent/CA1285511C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54101409A (en) * | 1977-11-14 | 1979-08-10 | Minnesota Mining & Mfg | Protein fixing agent |
| JPS56134989A (en) * | 1980-02-26 | 1981-10-22 | Tate & Lyle Ltd | Immobilized enzyme |
| JPS578785A (en) * | 1980-06-16 | 1982-01-18 | Nitto Electric Ind Co Ltd | Preparation of immobilized enzyme |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2617710A1 (fr) * | 1986-04-22 | 1989-01-13 | Dental Chem Co Ltd | Composition dentifrice |
| JPS6430584A (en) * | 1987-07-25 | 1989-02-01 | Ngk Insulators Ltd | Carrier for immobilizing organism catalyst |
| GB2224727B (en) * | 1988-09-29 | 1992-01-22 | Sangi Kk | Antimicrobial hydroxyapatite powders |
| GB2236676B (en) * | 1988-09-29 | 1992-01-22 | Sangi Kk | Antimicrobial hydroxyapatite powders |
| US5268174A (en) * | 1988-09-29 | 1993-12-07 | Kabushiki Kaisha Sangi | Antimicrobial hydroxyapatite powders containing hinokitiol, protamine or sorbic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH053275B2 (enExample) | 1993-01-14 |
| CA1285511C (en) | 1991-07-02 |
| US4812404A (en) | 1989-03-14 |
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