CA1285511C - Apatite immobilized glucanase and the like, and method of preparingthe same - Google Patents
Apatite immobilized glucanase and the like, and method of preparingthe sameInfo
- Publication number
- CA1285511C CA1285511C CA000517894A CA517894A CA1285511C CA 1285511 C CA1285511 C CA 1285511C CA 000517894 A CA000517894 A CA 000517894A CA 517894 A CA517894 A CA 517894A CA 1285511 C CA1285511 C CA 1285511C
- Authority
- CA
- Canada
- Prior art keywords
- apatite
- glucanase
- immobilized
- protein
- lysozyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229910052586 apatite Inorganic materials 0.000 title claims abstract description 72
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 32
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000007864 aqueous solution Substances 0.000 claims abstract description 10
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 24
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical group [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 24
- 108010001682 Dextranase Proteins 0.000 claims description 20
- 108010005131 levanase Proteins 0.000 claims description 14
- 108010000165 exo-1,3-alpha-glucanase Proteins 0.000 claims description 13
- 229910052587 fluorapatite Inorganic materials 0.000 claims description 13
- 108010014251 Muramidase Proteins 0.000 claims description 12
- 102000016943 Muramidase Human genes 0.000 claims description 12
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 12
- 229960000274 lysozyme Drugs 0.000 claims description 12
- 239000004325 lysozyme Substances 0.000 claims description 12
- 235000010335 lysozyme Nutrition 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 11
- 239000007853 buffer solution Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 5
- 230000003139 buffering effect Effects 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract description 39
- 108090000623 proteins and genes Proteins 0.000 abstract description 39
- 235000018102 proteins Nutrition 0.000 description 38
- 230000000694 effects Effects 0.000 description 18
- 108090000790 Enzymes Proteins 0.000 description 14
- 102000004190 Enzymes Human genes 0.000 description 14
- 229940088598 enzyme Drugs 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 108010093096 Immobilized Enzymes Proteins 0.000 description 8
- 238000005498 polishing Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 208000002925 dental caries Diseases 0.000 description 7
- 239000000551 dentifrice Substances 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 6
- 238000001179 sorption measurement Methods 0.000 description 6
- 239000003381 stabilizer Substances 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 229920002307 Dextran Polymers 0.000 description 5
- 238000013019 agitation Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000000354 decomposition reaction Methods 0.000 description 5
- 150000004676 glycans Polymers 0.000 description 5
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 5
- 239000005017 polysaccharide Substances 0.000 description 5
- 208000002064 Dental Plaque Diseases 0.000 description 4
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 4
- 230000003100 immobilizing effect Effects 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 150000001299 aldehydes Chemical class 0.000 description 3
- 230000001588 bifunctional effect Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 230000009849 deactivation Effects 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 239000008057 potassium phosphate buffer Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 108010088751 Albumins Proteins 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 102000018832 Cytochromes Human genes 0.000 description 2
- 108010052832 Cytochromes Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- ULDHMXUKGWMISQ-UHFFFAOYSA-N carvone Chemical compound CC(=C)C1CC=C(C)C(=O)C1 ULDHMXUKGWMISQ-UHFFFAOYSA-N 0.000 description 2
- 239000005018 casein Substances 0.000 description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 2
- 235000021240 caseins Nutrition 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 239000008055 phosphate buffer solution Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XUNKPNYCNUKOAU-VXJRNSOOSA-N (2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-amino-5-(diaminomethylideneamino)pentanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]a Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O XUNKPNYCNUKOAU-VXJRNSOOSA-N 0.000 description 1
- BSFODEXXVBBYOC-UHFFFAOYSA-N 8-[4-(dimethylamino)butan-2-ylamino]quinolin-6-ol Chemical compound C1=CN=C2C(NC(CCN(C)C)C)=CC(O)=CC2=C1 BSFODEXXVBBYOC-UHFFFAOYSA-N 0.000 description 1
- 239000005973 Carvone Substances 0.000 description 1
- 102000011632 Caseins Human genes 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- JLNTWVDSQRNWFU-UHFFFAOYSA-N OOOOOOO Chemical compound OOOOOOO JLNTWVDSQRNWFU-UHFFFAOYSA-N 0.000 description 1
- OZBZONOEYUBXTD-UHFFFAOYSA-N OOOOOOOOO Chemical compound OOOOOOOOO OZBZONOEYUBXTD-UHFFFAOYSA-N 0.000 description 1
- FYKAKDPHDGCKTF-UHFFFAOYSA-N OOOOOOOOOOOOOOOOOOO Chemical compound OOOOOOOOOOOOOOOOOOO FYKAKDPHDGCKTF-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 229910052622 kaolinite Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000012265 solid product Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/24—Phosphorous; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/18—Multi-enzyme systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Inorganic Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Cosmetics (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60206677A JPS6269988A (ja) | 1985-09-20 | 1985-09-20 | デキストラナーゼを安定に固定化したハイドロキシアパタイトの調整法 |
| JP206677/1985 | 1985-09-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1285511C true CA1285511C (en) | 1991-07-02 |
Family
ID=16527286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000517894A Expired - Fee Related CA1285511C (en) | 1985-09-20 | 1986-09-10 | Apatite immobilized glucanase and the like, and method of preparingthe same |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US4812404A (enExample) |
| JP (1) | JPS6269988A (enExample) |
| CA (1) | CA1285511C (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0228563B2 (ja) * | 1986-04-22 | 1990-06-25 | Dental Chem Co Ltd | Hamigakisoseibutsu |
| JPH0775543B2 (ja) * | 1987-07-25 | 1995-08-16 | 日本碍子株式会社 | 生体触媒固定化用担体 |
| US5268174A (en) * | 1988-09-29 | 1993-12-07 | Kabushiki Kaisha Sangi | Antimicrobial hydroxyapatite powders containing hinokitiol, protamine or sorbic acid |
| US5009898A (en) * | 1988-09-29 | 1991-04-23 | Kabushiki Kaisha Sangi | Antimicrobial hydroxyapatite powders and methods for preparing them |
| EP0425016B1 (en) * | 1989-10-27 | 1995-12-20 | The Procter & Gamble Company | Antimicrobial method and formulation employing type II endoglycosidase and antimicrobial agent |
| JP2004067547A (ja) * | 2002-08-02 | 2004-03-04 | Yoshinori Kuboki | チタン繊維医療材料 |
| CN100434907C (zh) * | 2006-09-28 | 2008-11-19 | 东北电力大学 | 细胞色素c分子自组装纳米有序复合结构组装体及制备方法 |
| ES2319059B1 (es) * | 2007-09-07 | 2010-02-12 | Universidad Complutense De Madrid | Brushita como sistema de inmovilizacion de enzimas y su uso en biosensores amperometricos biocompatibles. |
| JP4594439B1 (ja) * | 2009-12-29 | 2010-12-08 | 靖雄 知念 | 虫歯治療薬およびその製造方法 |
| CN111154744B (zh) * | 2019-12-24 | 2022-02-01 | 克劳丽化妆品股份有限公司 | 一种羟基磷灰石固定化α葡聚糖酶及其制备方法和应用 |
| CN112111479A (zh) * | 2020-09-30 | 2020-12-22 | 江苏海洋大学 | 一种右旋糖酐酶与羟基磷灰石复合材料及其制备方法和用途 |
| CN116999344A (zh) * | 2023-09-19 | 2023-11-07 | 余昭军 | 一种生物酶组合物及其制备方法及制得的牙膏 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4464468A (en) * | 1968-03-29 | 1984-08-07 | Agence Nationale De Valorisation De La Recherche (Anvar) | Immobilization of active protein by cross-linking to inactive protein |
| US4155546A (en) * | 1977-07-27 | 1979-05-22 | Itek Corporation | Bottom sheet feeding apparatus |
| US4210722A (en) * | 1977-11-14 | 1980-07-01 | Minnesota Mining And Manufacturing Company | Protein immobilizer |
| ZA811104B (en) * | 1980-02-26 | 1982-03-31 | Tate & Lyle Ltd | Immobilized enzymes, a process for their preparation and their use in converting substrates to products |
| JPS5810077B2 (ja) * | 1980-06-16 | 1983-02-24 | 日東電工株式会社 | 固定化酵素の製造方法 |
-
1985
- 1985-09-20 JP JP60206677A patent/JPS6269988A/ja active Granted
-
1986
- 1986-06-25 US US06/878,256 patent/US4812404A/en not_active Expired - Fee Related
- 1986-09-10 CA CA000517894A patent/CA1285511C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH053275B2 (enExample) | 1993-01-14 |
| US4812404A (en) | 1989-03-14 |
| JPS6269988A (ja) | 1987-03-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4812404A (en) | Apatite immobilized glucanase | |
| AU602149B2 (en) | Apatite immobilized glucanase and the like, and method of preparing the same | |
| JP2000502883A (ja) | 安定なアビジン組成物及びその使用方法 | |
| Nordbö et al. | Desorption of salivary proteins from hydroxyapatite by phytic acid and glycerophosphate and the plaque-inhibiting effect of the two compounds in vivo | |
| US4138476A (en) | Plaque dispersing enzymes as oral therapeutic agents by molecular alteration | |
| Smith et al. | Salivary myeloperoxidase of young adult humans | |
| US6726933B2 (en) | Rod-shaped mesoporous powder, humectant adsorbing powder, and cosmetic preparation using the same | |
| Fantl | Thiol groups of blood platelets in relation to clot retraction | |
| CA1281658C (en) | Dentifrice compositions | |
| EGGEN et al. | Surface properties of fluoride treated hydroxyapatite as judged by interactions with albumin and lysozyme | |
| Rølla | Adsorption of dextran to saliva-treated hydroxyapatite | |
| Voegel et al. | The adsorption of salivary glycoproteins to fluoride-treated synthetic hydroxyapatites | |
| Niwa et al. | The adsorptive properties of hydroxyapatite to albumin, dextran and lipids | |
| JPH0228563B2 (ja) | Hamigakisoseibutsu | |
| Edman et al. | Immobilized L-asparaginase-L-glutaminase from Acinetobacter glutaminasificans in microspheres: some properties in vivo and in an extracorporeal system | |
| CA2442524A1 (en) | Matrix stabilized enzyme crystals and methods of use | |
| WO1986004213A1 (en) | Disinfectant formulation and method of disinfection in vivo | |
| Christoffersen et al. | Adsorption of ovalbumin on calcium hydroxyapatite crystals | |
| Barkman et al. | An examination of the zinc requirement for the catalytic activity of 3-phosphoglyceraldehyde dehydrogenase | |
| JP2757946B2 (ja) | デキストラナーゼ吸着体およびその製造法 | |
| Davies et al. | Guinea pig kallikrein system | |
| JP3545063B2 (ja) | 体液の混濁除去方法 | |
| Knuuttila et al. | The interaction of collagenase with hydroxyapatite and related materials and enzymatic properties of the adsorbed enzyme | |
| Barker et al. | Absorption of a dextranase-concanavalin a conjugate on to hydroxyapatite | |
| FOUDA et al. | Characterization of human salivary β‐N‐acetyl‐D‐glucosaminidase (NAGase) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |