JPH10509317A - アスペルギルス中の融合体から得られるプロセッシングされた組換えラクトフェリンおよびラクトフェリンポリペプチド断片 - Google Patents

アスペルギルス中の融合体から得られるプロセッシングされた組換えラクトフェリンおよびラクトフェリンポリペプチド断片

Info

Publication number
JPH10509317A
JPH10509317A JP8515400A JP51540095A JPH10509317A JP H10509317 A JPH10509317 A JP H10509317A JP 8515400 A JP8515400 A JP 8515400A JP 51540095 A JP51540095 A JP 51540095A JP H10509317 A JPH10509317 A JP H10509317A
Authority
JP
Japan
Prior art keywords
lactoferrin
gene
derived
sequence
awamori
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP8515400A
Other languages
English (en)
Japanese (ja)
Inventor
コネリー，オーラ・エム
ヘドン，デニス・アール
オマリー，バート・ダブリュー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agennix Inc
Original Assignee
Agennix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agennix Inc filed Critical Agennix Inc
Publication of JPH10509317A publication Critical patent/JPH10509317A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Cosmetics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
JP8515400A 1994-11-02 1995-11-01 アスペルギルス中の融合体から得られるプロセッシングされた組換えラクトフェリンおよびラクトフェリンポリペプチド断片 Pending JPH10509317A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/303,009 US5571697A (en) 1989-05-05 1994-11-02 Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
US08/303,009 1994-11-02
PCT/US1995/014174 WO1996014413A1 (en) 1994-11-02 1995-11-01 Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in aspergillus

Publications (1)

Publication Number Publication Date
JPH10509317A true JPH10509317A (ja) 1998-09-14

Family

ID=23170177

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8515400A Pending JPH10509317A (ja) 1994-11-02 1995-11-01 アスペルギルス中の融合体から得られるプロセッシングされた組換えラクトフェリンおよびラクトフェリンポリペプチド断片

Country Status (11)

Country Link
US (3) US5571697A (enExample)
EP (1) EP0871724A1 (enExample)
JP (1) JPH10509317A (enExample)
KR (1) KR100443307B1 (enExample)
CN (1) CN1230537C (enExample)
AU (1) AU4279896A (enExample)
CA (1) CA2204246A1 (enExample)
DE (1) DE871724T1 (enExample)
ES (1) ES2127164T1 (enExample)
MX (1) MX9703244A (enExample)
WO (1) WO1996014413A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005531309A (ja) * 2002-06-28 2005-10-20 エラ、プランテック、ソシエダッド、リミターダ 植物の小胞体由来のタンパク粒に蓄積することによるペプチドおよびタンパク質の産生
JP2007504810A (ja) * 2003-09-11 2007-03-08 ノボザイムス アクティーゼルスカブ 抗微生物剤の組換え製造
JP2012524530A (ja) * 2009-04-22 2012-10-18 ディーエスエム アイピー アセッツ ビー．ブイ． 対象組換えポリペプチドの産生方法
JPWO2014007273A1 (ja) * 2012-07-03 2016-06-02 株式会社ジナリス 有用微生物および目的物質の製造方法

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US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
IL94183A (en) * 1989-05-05 2003-09-17 Baylor College Medicine cDNA SEQUENCE CODING FOR HUMAN LACTOFERRIN PROTEIN OR PORTION THEREOF AND LACTOFERRIN PROTEIN PRODUCED FROM SAID SEQUENCE
US6066469A (en) * 1990-03-08 2000-05-23 Ferro Dynamics, Inc. Cloning, expression, and uses of human lactoferrin
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
US6111081A (en) * 1996-05-31 2000-08-29 Baylor College Of Medicine Lactoferrin variants and uses thereof
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
WO2000020555A2 (en) * 1998-10-06 2000-04-13 Aarl Inc Transformation system in the field of filamentous fungal hosts: in chrysosporium
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
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US6592814B2 (en) * 1998-10-02 2003-07-15 Johnson & Johnson Vision Care, Inc. Biomedical devices with antimicrobial coatings
US6399570B1 (en) 1999-02-05 2002-06-04 Agennix, Inc. Antimicrobial/endotoxin neutralizing polypeptide
US7138150B2 (en) * 2000-05-02 2006-11-21 Ventria Bioscience Method of making an anti-infective composition for treating oral infections
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
US20080050503A1 (en) * 2000-05-02 2008-02-28 Ning Huang Expression of human milk proteins in transgenic plants
AU2002357934A1 (en) * 2001-12-28 2003-07-24 Campina B.V. Humanized lactoferrin and uses thereof
WO2003068934A2 (en) * 2002-02-14 2003-08-21 Rutter William J Chimeric molecules for cleavage in a treated host
PT1507554E (pt) * 2002-05-10 2011-11-21 Agennix Inc Lactoferrina no tratamento de neoplasmas malignos e outras doenças hiperproliferativas
US7097841B2 (en) * 2002-05-10 2006-08-29 New Century Pharmaceuticals, Inc. Ferritin fusion proteins for use in vaccines and other applications
AU2003233583A1 (en) * 2002-05-24 2003-12-12 Agennix Incorporated Oral lactoferrin in the treatment of respiratory disorders
CN1694720A (zh) * 2002-09-16 2005-11-09 阿根尼克斯有限公司 乳铁蛋白组合物和创伤治疗的方法
CA2508912A1 (en) * 2002-12-06 2004-06-24 Agennix Incorporated Oral lactoferrin in the treatment of sepsis
AU2003296447A1 (en) 2002-12-10 2004-06-30 Agennix Incorporated Lactoferrin as an agent in the prevention of organ transplant rejection and graft-versus-host-disease
US7323443B2 (en) * 2002-12-12 2008-01-29 Agennix Inc. Lactoferrin in the reduction of pain
WO2004103285A2 (en) * 2003-05-14 2004-12-02 Agennix Incorporated Lactoferrin in the treatment of diabetes mellitus
EP1635766A4 (en) * 2003-06-06 2009-09-30 Agennix Inc LACTOFERRIN AS ADJUVANS IN CANCER VACCINES
US20040259770A1 (en) * 2003-06-19 2004-12-23 Rush University Medical Center Method for determining leukocyte activation
WO2005018542A2 (en) * 2003-07-10 2005-03-03 Agennix Incorporated Use of lactoferrin in prophylaxis against infection and/or inflammation in immunosuppressed subjects
US20070274988A1 (en) * 2003-10-10 2007-11-29 Five Prime Therapeautics, Inc. Kiaa0779, Splice Variants Thereof, and Methods of Their Use
JP2007517504A (ja) * 2003-12-09 2007-07-05 ベントリア バイオサイエンス 融合担体として種子貯蔵蛋白を利用する植物種子中の融合ポリペプチドの高水準発現
WO2005085287A1 (en) * 2004-03-03 2005-09-15 En-N-Tech, Inc. Treatments for contaminant reduction in lactoferrin preparations and lactoferrin-containing compositions
US7183381B2 (en) 2004-10-26 2007-02-27 Agennix, Inc. Composition of lactoferrin related peptides and uses thereof
JP4634809B2 (ja) * 2005-01-05 2011-02-16 株式会社アップウェル アポタンパク質の製造方法
US20060211089A1 (en) * 2005-03-16 2006-09-21 Novozymes A/S Expression of defensins in filamentous fungi
CN101505781A (zh) 2006-06-22 2009-08-12 阿根尼克斯有限公司 作为辐射防护物质的乳铁蛋白
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
NZ555163A (en) 2007-05-14 2010-05-28 Fonterra Co Operative Group Methods of immune or hematological enhancement, inhibiting tumour formation or growth, and treating or preventing cancer, cancer symptoms, or the symptoms of cancer treatments
CA2695830A1 (en) 2007-08-08 2009-02-12 Novozymes Biopharma Dk A/S Transferrin variants and conjugates
CA2736661A1 (en) * 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
RU2579661C2 (ru) * 2009-01-28 2016-04-10 Жан-Поль ПЕРРОДЭН Способ производства лактоферрина, фракция, содержащая лактоферрин, и ее применения
WO2010089385A1 (en) 2009-02-06 2010-08-12 Novozymes Biopharma Dk A/S Purification process
WO2010092135A2 (en) 2009-02-11 2010-08-19 Novozymes Biopharma Uk Ltd. Albumin variants and conjugates
US20130225477A1 (en) 2010-02-25 2013-08-29 Agennix Ag Oral Lactoferrin in the Treatment of Severe Sepsis
CN103421794B (zh) * 2013-08-08 2014-07-02 华南理工大学 一种黑曲霉组成型表达元件、表达载体、重组黑曲霉及其构建方法和应用
CN108893415B (zh) * 2018-07-31 2022-12-27 上海凯圣生物科技有限公司 曲霉菌株及利用曲霉菌株发酵生产人乳铁蛋白的方法
EP4587565A1 (en) * 2022-09-15 2025-07-23 Novozymes A/S Fungal signal peptides
WO2025157268A1 (zh) * 2024-01-24 2025-07-31 南京百斯杰生物工程有限公司 一种米曲霉重组菌株的构建方法
WO2025226596A1 (en) * 2024-04-24 2025-10-30 Novozymes A/S Methods for producing secreted polypeptides
CN119776443B (zh) * 2024-12-23 2025-12-02 江苏创健医疗科技股份有限公司 一种多拷贝整合实现高效合成人乳铁蛋白的方法与应用
CN119841906B (zh) * 2025-01-26 2025-09-26 新苡萃(上海)生物科技有限公司 一种淀粉酶信号肽变体及其应用

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005531309A (ja) * 2002-06-28 2005-10-20 エラ、プランテック、ソシエダッド、リミターダ 植物の小胞体由来のタンパク粒に蓄積することによるペプチドおよびタンパク質の産生
JP2007504810A (ja) * 2003-09-11 2007-03-08 ノボザイムス アクティーゼルスカブ 抗微生物剤の組換え製造
JP2012524530A (ja) * 2009-04-22 2012-10-18 ディーエスエム アイピー アセッツ ビー．ブイ． 対象組換えポリペプチドの産生方法
JPWO2014007273A1 (ja) * 2012-07-03 2016-06-02 株式会社ジナリス 有用微生物および目的物質の製造方法
US10047382B2 (en) 2012-07-03 2018-08-14 Kao Corporation Useful microorganism and method for producing substance of interest
US10781461B2 (en) 2012-07-03 2020-09-22 Kao Corporation Useful microorganism and method for producing substance of interest

Also Published As

Publication number Publication date
CN1230537C (zh) 2005-12-07
MX9703244A (es) 1998-07-31
KR970707283A (ko) 1997-12-01
US6080559A (en) 2000-06-27
DE871724T1 (de) 1999-06-02
ES2127164T1 (es) 1999-04-16
CN1171815A (zh) 1998-01-28
US5955316A (en) 1999-09-21
WO1996014413A1 (en) 1996-05-17
EP0871724A4 (enExample) 1998-10-21
AU4279896A (en) 1996-05-31
EP0871724A1 (en) 1998-10-21
US5571697A (en) 1996-11-05
CA2204246A1 (en) 1996-05-17
KR100443307B1 (ko) 2004-11-08

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