DE871724T1 - Expression von prozessierten, rekombinierten lactoferrin und dessen polypeptiden durch ein fusionsprodukt in aspergillus - Google Patents

Expression von prozessierten, rekombinierten lactoferrin und dessen polypeptiden durch ein fusionsprodukt in aspergillus

Info

Publication number
DE871724T1
DE871724T1 DE0871724T DE95941350T DE871724T1 DE 871724 T1 DE871724 T1 DE 871724T1 DE 0871724 T DE0871724 T DE 0871724T DE 95941350 T DE95941350 T DE 95941350T DE 871724 T1 DE871724 T1 DE 871724T1
Authority
DE
Germany
Prior art keywords
lactoferrine
recombined
aspergillus
polypeptides
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE0871724T
Other languages
English (en)
Inventor
Orla Conneely
Denis R Headon
Bert O'malley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agennix Inc
Original Assignee
Agennix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agennix Inc filed Critical Agennix Inc
Publication of DE871724T1 publication Critical patent/DE871724T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE0871724T 1994-11-02 1995-11-01 Expression von prozessierten, rekombinierten lactoferrin und dessen polypeptiden durch ein fusionsprodukt in aspergillus Pending DE871724T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/303,009 US5571697A (en) 1989-05-05 1994-11-02 Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
PCT/US1995/014174 WO1996014413A1 (en) 1994-11-02 1995-11-01 Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in aspergillus

Publications (1)

Publication Number Publication Date
DE871724T1 true DE871724T1 (de) 1999-06-02

Family

ID=23170177

Family Applications (1)

Application Number Title Priority Date Filing Date
DE0871724T Pending DE871724T1 (de) 1994-11-02 1995-11-01 Expression von prozessierten, rekombinierten lactoferrin und dessen polypeptiden durch ein fusionsprodukt in aspergillus

Country Status (11)

Country Link
US (3) US5571697A (de)
EP (1) EP0871724A1 (de)
JP (1) JPH10509317A (de)
KR (1) KR100443307B1 (de)
CN (1) CN1230537C (de)
AU (1) AU4279896A (de)
CA (1) CA2204246A1 (de)
DE (1) DE871724T1 (de)
ES (1) ES2127164T1 (de)
MX (1) MX9703244A (de)
WO (1) WO1996014413A1 (de)

Families Citing this family (50)

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US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
IL94183A (en) * 1989-05-05 2003-09-17 Baylor College Medicine cDNA SEQUENCE CODING FOR HUMAN LACTOFERRIN PROTEIN OR PORTION THEREOF AND LACTOFERRIN PROTEIN PRODUCED FROM SAID SEQUENCE
US6066469A (en) 1990-03-08 2000-05-23 Ferro Dynamics, Inc. Cloning, expression, and uses of human lactoferrin
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
US6111081A (en) * 1996-05-31 2000-08-29 Baylor College Of Medicine Lactoferrin variants and uses thereof
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
EP1104481B1 (de) * 1998-08-19 2011-04-20 Monsanto Technology LLC Pflanzliche expressionsvektoren
US6592814B2 (en) * 1998-10-02 2003-07-15 Johnson & Johnson Vision Care, Inc. Biomedical devices with antimicrobial coatings
KR100618495B1 (ko) * 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US6399570B1 (en) 1999-02-05 2002-06-04 Agennix, Inc. Antimicrobial/endotoxin neutralizing polypeptide
US20080050503A1 (en) * 2000-05-02 2008-02-28 Ning Huang Expression of human milk proteins in transgenic plants
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
US7138150B2 (en) * 2000-05-02 2006-11-21 Ventria Bioscience Method of making an anti-infective composition for treating oral infections
EP1478379A4 (de) * 2001-12-28 2005-12-21 Campina Bv Humanisiertes lactoferrin und seine anwendungen
WO2003068934A2 (en) * 2002-02-14 2003-08-21 Rutter William J Chimeric molecules for cleavage in a treated host
US7097841B2 (en) * 2002-05-10 2006-08-29 New Century Pharmaceuticals, Inc. Ferritin fusion proteins for use in vaccines and other applications
ATE519499T1 (de) * 2002-05-10 2011-08-15 Agennix Inc Lactoferrin bei der behandlung maligner neoplasien und anderer hyperproliferativer erkrankungen
WO2003099207A2 (en) * 2002-05-24 2003-12-04 Agennix Incorporated Oral lactoferrin in the treatment of respiratory disorders
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
CA2499014A1 (en) 2002-09-16 2004-03-25 Agennix Incorporated Lactoferrin compositions and methods of wound treatment
EP1581243A4 (de) * 2002-12-06 2008-01-02 Agennix Inc Orales lactoferrin für sepsisbehandlung
US7592306B2 (en) 2002-12-10 2009-09-22 Agennix, Inc. Lactoferrin as an agent in the prevention of organ transplant rejection and graft-versus-host-disease
WO2004054608A2 (en) * 2002-12-12 2004-07-01 Agennix Incorporated Lactoferrin in the reduction of pain
WO2004103285A2 (en) * 2003-05-14 2004-12-02 Agennix Incorporated Lactoferrin in the treatment of diabetes mellitus
EP1635766A4 (de) * 2003-06-06 2009-09-30 Agennix Inc Lactoferrin als adjuvans in krebs-vakzinen
US20040259770A1 (en) * 2003-06-19 2004-12-23 Rush University Medical Center Method for determining leukocyte activation
US20050075277A1 (en) * 2003-07-10 2005-04-07 Agennix Incorporated Use of lactoferrin in prophylaxis against infection and/or inflammation in immunosuppressed subjects
EP1680503B1 (de) * 2003-09-11 2011-06-22 Novozymes Adenium Biotech A/S Rekombinante Herstellung antimikrobieller Agentien
US20070274988A1 (en) * 2003-10-10 2007-11-29 Five Prime Therapeautics, Inc. Kiaa0779, Splice Variants Thereof, and Methods of Their Use
WO2005056578A2 (en) * 2003-12-09 2005-06-23 Ventria Bioscience High-level expression of fusion polypeptides in plant seeds utilizing seed-storage proteins as fusion carriers
WO2005085287A1 (en) * 2004-03-03 2005-09-15 En-N-Tech, Inc. Treatments for contaminant reduction in lactoferrin preparations and lactoferrin-containing compositions
WO2006047744A2 (en) * 2004-10-26 2006-05-04 Agennix Incorporated Compositions of lactoferrin related peptides and uses thereof
JP4634809B2 (ja) * 2005-01-05 2011-02-16 株式会社アップウェル アポタンパク質の製造方法
US20060211089A1 (en) * 2005-03-16 2006-09-21 Novozymes A/S Expression of defensins in filamentous fungi
EP2049135A4 (de) 2006-06-22 2009-12-30 Agennix Inc Lactoferrin als radioprotektives mittel
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
NZ555163A (en) 2007-05-14 2010-05-28 Fonterra Co Operative Group Methods of immune or hematological enhancement, inhibiting tumour formation or growth, and treating or preventing cancer, cancer symptoms, or the symptoms of cancer treatments
WO2009019314A1 (en) 2007-08-08 2009-02-12 Novozymes A/S Transferrin variants and conjugates
CA2736661A1 (en) * 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
JP5718254B2 (ja) * 2009-01-28 2015-05-13 ペローデイン，ジヤン−ポール ラクトフェリンの製造方法
EP2393834B1 (de) 2009-02-06 2013-04-17 Novozymes Biopharma DK A/S Aufreinigungsverfahren
US9493545B2 (en) 2009-02-11 2016-11-15 Albumedix A/S Albumin variants and conjugates
AU2010241099A1 (en) * 2009-04-22 2011-10-27 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
US20130225477A1 (en) 2010-02-25 2013-08-29 Agennix Ag Oral Lactoferrin in the Treatment of Severe Sepsis
WO2014007273A1 (ja) 2012-07-03 2014-01-09 株式会社ジナリス 有用微生物および目的物質の製造方法
CN103421794B (zh) * 2013-08-08 2014-07-02 华南理工大学 一种黑曲霉组成型表达元件、表达载体、重组黑曲霉及其构建方法和应用
CN108893415B (zh) * 2018-07-31 2022-12-27 上海凯圣生物科技有限公司 曲霉菌株及利用曲霉菌株发酵生产人乳铁蛋白的方法
WO2024056643A1 (en) * 2022-09-15 2024-03-21 Novozymes A/S Fungal signal peptides

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IL94183A (en) * 1989-05-05 2003-09-17 Baylor College Medicine cDNA SEQUENCE CODING FOR HUMAN LACTOFERRIN PROTEIN OR PORTION THEREOF AND LACTOFERRIN PROTEIN PRODUCED FROM SAID SEQUENCE
US5571691A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Production of recombinant lactoferrin and lactoferrin polypeptides using CDNA sequences in various organisms
JP3153234B2 (ja) * 1989-06-16 2001-04-03 ジェネンコー インコーポレーテッド 糸状菌からの目的とするポリペプチドの分泌を増幅するｄｎａ配列、ベクター、及び融合ポリペプチド
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Also Published As

Publication number Publication date
AU4279896A (en) 1996-05-31
WO1996014413A1 (en) 1996-05-17
KR970707283A (ko) 1997-12-01
ES2127164T1 (es) 1999-04-16
US5955316A (en) 1999-09-21
CA2204246A1 (en) 1996-05-17
CN1230537C (zh) 2005-12-07
EP0871724A1 (de) 1998-10-21
US5571697A (en) 1996-11-05
US6080559A (en) 2000-06-27
EP0871724A4 (de) 1998-10-21
CN1171815A (zh) 1998-01-28
KR100443307B1 (ko) 2004-11-08
JPH10509317A (ja) 1998-09-14
MX9703244A (es) 1998-07-31

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