ES2127164T1 - Expresion de lactoferrina recombinante y fragmentos polipeptidicos de lactoferrina procesados a partir de un producto de fusion en aspergillus. - Google Patents

Expresion de lactoferrina recombinante y fragmentos polipeptidicos de lactoferrina procesados a partir de un producto de fusion en aspergillus.

Info

Publication number
ES2127164T1
ES2127164T1 ES95941350T ES95941350T ES2127164T1 ES 2127164 T1 ES2127164 T1 ES 2127164T1 ES 95941350 T ES95941350 T ES 95941350T ES 95941350 T ES95941350 T ES 95941350T ES 2127164 T1 ES2127164 T1 ES 2127164T1
Authority
ES
Spain
Prior art keywords
lactoferrin
aspergillus
expression
polypeptide fragments
fusion product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
ES95941350T
Other languages
English (en)
Inventor
Orla M Conneely
Denis R Lishin Aghnacur Headon
Bert W O'malley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agennix Inc
Original Assignee
Agennix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agennix Inc filed Critical Agennix Inc
Publication of ES2127164T1 publication Critical patent/ES2127164T1/es
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LA PRESENTE INVENCION DESCRIBE LA PRODUCCION DE LACTOFERRINAS Y DE FRAGMENTOS DEL POLIPEPTIDO DE LA LACTOFERRINA UTILIZANDO LAS CELULAS HUESPED ASPERGILLUS JUNTO CON NUEVOS CONSTRUCTOS DE PLASMIDOS. DE FORMA MAS ESPECIFICA, LA PRESENTE INVENCION DESCRIBE NUEVOS CONSTRUCTOS VECTORES CAPACES DE PRODUCIR LACTOFERRINAS Y FRAGMENTOS DEL POLIPEPTIDO DE LA LACTOFERRINA EN CELULAS HUESPED ASPERGILLUS. MAS CONCRETAMENTE, LA PRESENTE INVENCION DESCRIBE NUEVOS CONSTRUCTOS DE PLASMIDOS QUE RESULTAN ADECUADOS PARA SU USO CON CELULAS HUESPED ASPERGILLUS, Y EN ESPECIAL ASPERGILLUS AWAMORI, A. NIGER Y A. ORYZAE, QUE LAS PERMITE PRODUCIR GRANDES CANTIDADES DE LACTOFERRINAS RECOMBINANTES Y DE FRAGMENTOS DEL POLIPEPTIDO DE LA LACTOFERRINA.
ES95941350T 1994-11-02 1995-11-01 Expresion de lactoferrina recombinante y fragmentos polipeptidicos de lactoferrina procesados a partir de un producto de fusion en aspergillus. Pending ES2127164T1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/303,009 US5571697A (en) 1989-05-05 1994-11-02 Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus

Publications (1)

Publication Number Publication Date
ES2127164T1 true ES2127164T1 (es) 1999-04-16

Family

ID=23170177

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95941350T Pending ES2127164T1 (es) 1994-11-02 1995-11-01 Expresion de lactoferrina recombinante y fragmentos polipeptidicos de lactoferrina procesados a partir de un producto de fusion en aspergillus.

Country Status (11)

Country Link
US (3) US5571697A (es)
EP (1) EP0871724A1 (es)
JP (1) JPH10509317A (es)
KR (1) KR100443307B1 (es)
CN (1) CN1230537C (es)
AU (1) AU4279896A (es)
CA (1) CA2204246A1 (es)
DE (1) DE871724T1 (es)
ES (1) ES2127164T1 (es)
MX (1) MX9703244A (es)
WO (1) WO1996014413A1 (es)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
IL94183A (en) * 1989-05-05 2003-09-17 Baylor College Medicine cDNA SEQUENCE CODING FOR HUMAN LACTOFERRIN PROTEIN OR PORTION THEREOF AND LACTOFERRIN PROTEIN PRODUCED FROM SAID SEQUENCE
US6066469A (en) * 1990-03-08 2000-05-23 Ferro Dynamics, Inc. Cloning, expression, and uses of human lactoferrin
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
US6111081A (en) * 1996-05-31 2000-08-29 Baylor College Of Medicine Lactoferrin variants and uses thereof
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
CZ302074B6 (cs) * 1998-08-19 2010-09-29 Monsanto Technology Llc Rostlinné expresní vektory, transformované rostlinné bunky a zpusob pro zlepšení genové exprese v rostlinách
US6592814B2 (en) * 1998-10-02 2003-07-15 Johnson & Johnson Vision Care, Inc. Biomedical devices with antimicrobial coatings
EP1117808B1 (en) * 1998-10-06 2004-12-29 Mark Aaron Emalfarb Transformation system in the field of filamentous fungal hosts: in chrysosporium
US6399570B1 (en) 1999-02-05 2002-06-04 Agennix, Inc. Antimicrobial/endotoxin neutralizing polypeptide
US7138150B2 (en) * 2000-05-02 2006-11-21 Ventria Bioscience Method of making an anti-infective composition for treating oral infections
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
US20080050503A1 (en) * 2000-05-02 2008-02-28 Ning Huang Expression of human milk proteins in transgenic plants
EP1478379A4 (en) * 2001-12-28 2005-12-21 Campina Bv HUMANIZED LACTOFERRIN AND ITS APPLICATIONS
CA2475388A1 (en) * 2002-02-14 2003-08-21 William J. Rutter Chimeric molecules for cleavage in a treated host
CN105566497A (zh) * 2002-05-10 2016-05-11 新世纪药品有限公司 融合铁蛋白在疫苗和其他方面的应用
WO2003094952A1 (en) * 2002-05-10 2003-11-20 Agennix Incorporated Intratumorally administered lactoferrin in the treatment of malignant neoplasms and other hyperproliferative diseases
AU2003233583A1 (en) * 2002-05-24 2003-12-12 Agennix Incorporated Oral lactoferrin in the treatment of respiratory disorders
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
WO2004024180A1 (en) 2002-09-16 2004-03-25 Agennix Incorporated Lactoferrin compositions and methods of wound treatment
AU2003298906A1 (en) * 2002-12-06 2004-06-30 Agennix Incorporated Oral lactoferrin in the treatment of sepsis
AU2003296447A1 (en) * 2002-12-10 2004-06-30 Agennix Incorporated Lactoferrin as an agent in the prevention of organ transplant rejection and graft-versus-host-disease
US7323443B2 (en) * 2002-12-12 2008-01-29 Agennix Inc. Lactoferrin in the reduction of pain
US7034126B2 (en) * 2003-05-14 2006-04-25 Agennix, Inc. Lactoferrin in the treatment of diabetes mellitus
WO2005046571A2 (en) * 2003-06-06 2005-05-26 Agennix Incorporated Lactoferrin as an adjuvant in cancer vaccines
US20040259770A1 (en) * 2003-06-19 2004-12-23 Rush University Medical Center Method for determining leukocyte activation
US20050075277A1 (en) * 2003-07-10 2005-04-07 Agennix Incorporated Use of lactoferrin in prophylaxis against infection and/or inflammation in immunosuppressed subjects
BRPI0413833A (pt) 2003-09-11 2006-10-24 Novozymes As célula hospedeira microbiana recombinante, construção de ácido nucleico, método para produzir uma enzima e/ou um agente antimicrobiano, produto de fusão, aditivo e composição de ração animal, animal não humano transgênico ou produtos ou elementos deste, usos do produto de fusão, da co-expressão de um agente antimicrobiano e uma enzima, e de um peptìdeo de proteção, e, método para identificar um peptìdeo de proteção
US20070274988A1 (en) * 2003-10-10 2007-11-29 Five Prime Therapeautics, Inc. Kiaa0779, Splice Variants Thereof, and Methods of Their Use
JP2007517504A (ja) * 2003-12-09 2007-07-05 ベントリア バイオサイエンス 融合担体として種子貯蔵蛋白を利用する植物種子中の融合ポリペプチドの高水準発現
US7125963B2 (en) * 2004-03-03 2006-10-24 En N Tech Inc Treatments for contaminant reduction in lactoferrin preparations and lactoferrin containing compositions
WO2006047744A2 (en) * 2004-10-26 2006-05-04 Agennix Incorporated Compositions of lactoferrin related peptides and uses thereof
JP4634809B2 (ja) * 2005-01-05 2011-02-16 株式会社アップウェル アポタンパク質の製造方法
US20060211089A1 (en) * 2005-03-16 2006-09-21 Novozymes A/S Expression of defensins in filamentous fungi
WO2007150049A2 (en) 2006-06-22 2007-12-27 Agennix Incorporated Lactoferrin as a radioprotective agent
EP2102366A4 (en) 2006-12-10 2010-01-27 Dyadic International Inc EXPRESSION AND HIGH-DROP SCREENING OF COMPLEX EXPRESSED DNA LIBRARIES IN FADENED MUSHROOMS
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
NZ555163A (en) 2007-05-14 2010-05-28 Fonterra Co Operative Group Methods of immune or hematological enhancement, inhibiting tumour formation or growth, and treating or preventing cancer, cancer symptoms, or the symptoms of cancer treatments
EP2604623A3 (en) 2007-08-08 2013-10-02 Novozymes Biopharma DK A/S Transferrin variants and conjugates
WO2009033071A2 (en) * 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
WO2010112988A1 (en) * 2009-01-28 2010-10-07 Jean-Paul Perraudin Method for production of lactoferrin
WO2010089385A1 (en) 2009-02-06 2010-08-12 Novozymes Biopharma Dk A/S Purification process
EP3243835B1 (en) 2009-02-11 2024-04-10 Albumedix Ltd Albumin variants and conjugates
JP2012524530A (ja) * 2009-04-22 2012-10-18 ディーエスエム アイピー アセッツ ビー．ブイ． 対象組換えポリペプチドの産生方法
WO2011104352A1 (en) 2010-02-25 2011-09-01 Agennix Ag Oral lactoferrin in the treatment of severe sepsis
EP2851421B1 (en) 2012-07-03 2018-11-28 Kao Corporation Useful microorganism, and method for producing substance of interest
CN103421794B (zh) * 2013-08-08 2014-07-02 华南理工大学 一种黑曲霉组成型表达元件、表达载体、重组黑曲霉及其构建方法和应用
CN108893415B (zh) * 2018-07-31 2022-12-27 上海凯圣生物科技有限公司 曲霉菌株及利用曲霉菌株发酵生产人乳铁蛋白的方法
WO2024056643A1 (en) * 2022-09-15 2024-03-21 Novozymes A/S Fungal signal peptides

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766075A (en) * 1982-07-14 1988-08-23 Genentech, Inc. Human tissue plasminogen activator
US4652639A (en) * 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
US4703008A (en) * 1983-12-13 1987-10-27 Kiren-Amgen, Inc. DNA sequences encoding erythropoietin
US4710465A (en) * 1984-04-19 1987-12-01 Yale University Junction-fragment DNA probes and probe clusters
US4886747A (en) * 1985-03-22 1989-12-12 Genentech, Inc. Nucleic acid encoding TGF-β and its uses
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
US4965190A (en) * 1986-07-31 1990-10-23 Howard Hughes Medical Institute Methods for the identification of mutations in the human phenylalanine hydroxylase gene using DNA probes
US5019508A (en) * 1987-08-27 1991-05-28 Biotechnology Research Partners, Ltd. Synovial phospholipases
US5081227A (en) * 1988-03-04 1992-01-14 La Jolla Cancer Research Foundation Germ cell alkaline phosphatase
IL94183A (en) * 1989-05-05 2003-09-17 Baylor College Medicine cDNA SEQUENCE CODING FOR HUMAN LACTOFERRIN PROTEIN OR PORTION THEREOF AND LACTOFERRIN PROTEIN PRODUCED FROM SAID SEQUENCE
US5571691A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Production of recombinant lactoferrin and lactoferrin polypeptides using CDNA sequences in various organisms
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
AU627334B2 (en) * 1989-06-16 1992-08-20 Genencor Inc. Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
UA27702C2 (uk) * 1989-09-27 2000-10-16 Гіст-Брокейдс Н.В. Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger
IL95697A0 (en) * 1989-09-28 1991-06-30 Granada Biosciences Inc Cdna sequence for porcine lactoferrin protein
WO1991013982A1 (en) * 1990-03-08 1991-09-19 Ferrodynamics, Inc. Genetically engineered human lactoferrin
JP2818056B2 (ja) * 1990-09-07 1998-10-30 森永乳業株式会社 抗菌性ペプチドおよび抗菌剤
JPH07502723A (ja) * 1991-02-08 1995-03-23 ザ・ユニバーシテイ・オブ・バーモント・アンド・ステイト・アグリカルチユラル・カレツジ 組み替えトランスフェリン、トランスフェリン半−分子、及びそれらの突然変異体
ES2159519T3 (es) * 1991-12-09 2001-10-16 Unilever Nv Procedimiento para producir/secretar una proteina por un moho transformado usando regiones de regulacion de la expresion/secrecion derivadas de un gen de endoxilanasa ii de aspergillus.
ZA932568B (en) * 1992-04-24 1993-11-12 Baylor College Midecine A Non Production of recombinant human lactoferrin

Also Published As

Publication number Publication date
JPH10509317A (ja) 1998-09-14
EP0871724A4 (es) 1998-10-21
CN1230537C (zh) 2005-12-07
US5955316A (en) 1999-09-21
WO1996014413A1 (en) 1996-05-17
MX9703244A (es) 1998-07-31
US5571697A (en) 1996-11-05
KR100443307B1 (ko) 2004-11-08
CA2204246A1 (en) 1996-05-17
KR970707283A (ko) 1997-12-01
US6080559A (en) 2000-06-27
EP0871724A1 (en) 1998-10-21
CN1171815A (zh) 1998-01-28
AU4279896A (en) 1996-05-31
DE871724T1 (de) 1999-06-02

Similar Documents

Publication Publication Date Title
ES2127164T1 (es) Expresion de lactoferrina recombinante y fragmentos polipeptidicos de lactoferrina procesados a partir de un producto de fusion en aspergillus.
DE69323879T2 (de) Herstellung von rekombinanten, menschlichem lactoferrin
ES2061632T3 (es) Kluyveromyces como celula hospedante.
WO2020014285A3 (en) Fusion constructs and methods of using thereof
CR20110556A (es) Proteinas de fusion rage y metodos de empleo
EP0330451A3 (en) A cdna coding for human normal serum albumin a, and a process for production of the albumin
CY1116110T1 (el) ΔΙΑΛΥΤΗ ΓΛΥΚΟΠΡΩΤΕΪΝΗ ΥΑΛΟΥΡΟΝΙΔΑΣΗΣ (sHASEGP), ΔΙΑΔΙΚΑΣΙΑ ΓΙΑ ΤΗΝ ΠΑΡΑΣΚΕΥΗ ΤΗΣ, ΧΡΗΣΕΙΣ ΚΑΙ ΦΑΡΜΑΚΕΥΤΙΚΕΣ ΣΥΝΘΕΣΕΙΣ ΠΟΥ ΤΗΝ ΠΕΡΙΛΑΜΒΑΝΟΥΝ
AU2152897A (en) Alkaline protease deficient filamentous fungi
AR017515A1 (es) Metodos para preparar un transgen que codifica para la produccion de oligosacaridos y glicoproteinas en la leche de un mamifero no humano, laleche producida por dicho mamifero, una fraccion de la misma, un oligosacarido o glicoproteina, un producto nutricional, y el uso de oligosacaridos yglicoprote
ATE254175T1 (de) Mit einem neuen plasmid transformierter e.coli stamm jm83/pkp2 und damit hergestellte phytase
IL102783A0 (en) Dna sequence encoding bovine alpha-lactalbumin and methods of use
PT1610726E (pt) Lente intra-ocular e seu injector
ES2159519T3 (es) Procedimiento para producir/secretar una proteina por un moho transformado usando regiones de regulacion de la expresion/secrecion derivadas de un gen de endoxilanasa ii de aspergillus.
WO2020167455A3 (en) Platform for producing glycoproteins, identifying glycosylation pathways
DE60222759D1 (de) Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem
ZA9010188B (en) Bcrf1 proteins as inhibitors of interferon-gamma
ES2059911T3 (es) Un procedimiento para la produccion de proteinas vegetales hidrolizadas y el producto del mismo.
ES2172517T3 (es) Promotor bovino de estres por calor y usos del mismo.
MX2022003531A (es) Polipeptidos de fusion dap10/dap12.
WO2004055041A3 (en) Defensin-inducing peptides from fusobacterium
FI972100A (fi) Nisiinivariantin tuottaminen
IT1292751B1 (it) Procedimento per realizzare capsule monopezzo in materiale plastico.
DE69841995D1 (de) Säugetier milchwachstumsfaktor
WO2020257942A8 (en) A method for increasing yields and the specific activity of certain recombinant proteins in mammalian cells by co-expressing full-length furin
ES2105987B1 (es) Producto alimenticio tipo bizcocho, magdalena o similar y procedimiento para la obtencion del mismo.