DE60222759D1 - Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem - Google Patents

Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem

Info

Publication number
DE60222759D1
DE60222759D1 DE60222759T DE60222759T DE60222759D1 DE 60222759 D1 DE60222759 D1 DE 60222759D1 DE 60222759 T DE60222759 T DE 60222759T DE 60222759 T DE60222759 T DE 60222759T DE 60222759 D1 DE60222759 D1 DE 60222759D1
Authority
DE
Germany
Prior art keywords
protein synthesis
protein
cell
detergent
free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60222759T
Other languages
English (en)
Other versions
DE60222759T2 (de
Inventor
Kaori Tajima
Takanori Kigawa
Mikako Shirouzu
Takashi Yabuki
Goushi Ishihara
Shigeyuki Yokoyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN Institute of Physical and Chemical Research
Original Assignee
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN Institute of Physical and Chemical Research filed Critical RIKEN Institute of Physical and Chemical Research
Publication of DE60222759D1 publication Critical patent/DE60222759D1/de
Application granted granted Critical
Publication of DE60222759T2 publication Critical patent/DE60222759T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE60222759T 2001-05-02 2002-04-26 Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem Expired - Lifetime DE60222759T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2001135111 2001-05-02
JP2001135111 2001-05-02
JP2001285145A JP4838957B2 (ja) 2001-05-02 2001-09-19 無細胞タンパク質合成系を用いたタンパク質の製造方法
JP2001285145 2001-09-19
PCT/JP2002/004204 WO2002090537A1 (en) 2001-05-02 2002-04-26 Process for synthesizing protein using cell-free protein synthesis system

Publications (2)

Publication Number Publication Date
DE60222759D1 true DE60222759D1 (de) 2007-11-15
DE60222759T2 DE60222759T2 (de) 2008-07-03

Family

ID=26614632

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60222759T Expired - Lifetime DE60222759T2 (de) 2001-05-02 2002-04-26 Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem

Country Status (7)

Country Link
US (1) US7045593B2 (de)
EP (1) EP1384778B1 (de)
JP (1) JP4838957B2 (de)
AT (1) ATE374814T1 (de)
CA (1) CA2446031A1 (de)
DE (1) DE60222759T2 (de)
WO (1) WO2002090537A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1507003B1 (de) 2002-05-22 2011-09-21 Riken Verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung eines thioredoxinfusionsprotein-expressionsvektors
JP4441170B2 (ja) 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
JP2008514240A (ja) * 2004-10-01 2008-05-08 インヴィトロジェン コーポレーション 生体分子のインビトロ合成用の供給バッファー、系、及び方法
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4787488B2 (ja) 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
JP4785174B2 (ja) * 2004-12-10 2011-10-05 株式会社豊田中央研究所 膜タンパク質複合材料及びその製造方法
US20060216762A1 (en) * 2005-03-24 2006-09-28 Bayer Healthcare Llc Extracting reagent for hydrophobic analyte in whole blood
JP4854415B2 (ja) * 2006-07-25 2012-01-18 花王株式会社 界面活性剤によるプロテアーゼ活性化制御
EP2213747B1 (de) * 2007-11-05 2021-06-30 Riken Verfahren zur herstellung eines membranproteins
JP2010132594A (ja) * 2008-12-04 2010-06-17 Tokyo Institute Of Technology 膜タンパク質の固定化方法
JP6706813B2 (ja) 2015-06-30 2020-06-10 住友ゴム工業株式会社 膜結合蛋白質を結合させたゴム粒子の製造方法、空気入りタイヤの製造方法及びゴム製品の製造方法
JP6586693B2 (ja) 2015-06-30 2019-10-09 住友ゴム工業株式会社 ポリイソプレノイドの製造方法、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法
EP3309257B1 (de) 2015-06-30 2020-08-05 Sumitomo Rubber Industries, Ltd. Herstellungsverfahren für polyisoprenoid, vektor, transgene pflanze, herstellungsverfahren für luftreifen und herstellungsverfahren für gummiprodukt
US11618777B2 (en) 2015-07-31 2023-04-04 Shigeyuki Yokoyama Method of manufacturing membrane protein and utilization thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270181A (en) * 1991-02-06 1993-12-14 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
JP3092904B2 (ja) 1995-10-13 2000-09-25 株式会社エイチ・エス・ピー研究所 細菌により可溶性蛋白質を生産する方法
EP1496120B1 (de) * 1997-07-07 2007-03-28 Medical Research Council Ein in vitro Sortierverfahren
JP2000175695A (ja) * 1998-12-14 2000-06-27 Inst Of Physical & Chemical Res 無細胞タンパク質合成系によるポリペプチドの製造方法
AU765632B2 (en) * 1999-05-11 2003-09-25 Cellfree Sciences Co., Ltd. Preparation containing cell extract for synthesizing cell-free protein and means for synthesizing cell-free protein
KR100892889B1 (ko) 2000-12-26 2009-04-15 세키스이가가쿠 고교가부시키가이샤 재조합 단백질의 생산방법 및 융합 단백질
JP2002262873A (ja) * 2001-03-06 2002-09-17 Inst Of Physical & Chemical Res タンパク質ドメインの製造方法及びその方法により得られたドメインを用いるタンパク質の立体構造解析方法

Also Published As

Publication number Publication date
US20040203091A1 (en) 2004-10-14
EP1384778A1 (de) 2004-01-28
US7045593B2 (en) 2006-05-16
EP1384778B1 (de) 2007-10-03
JP4838957B2 (ja) 2011-12-14
JP2003018999A (ja) 2003-01-21
WO2002090537A1 (en) 2002-11-14
ATE374814T1 (de) 2007-10-15
EP1384778A4 (de) 2004-06-16
DE60222759T2 (de) 2008-07-03
CA2446031A1 (en) 2002-11-14

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