WO2000068416A3 - Methoden zur auffindung von proteasen, die spezifisch membrangebundene substrate spalten - Google Patents

Methoden zur auffindung von proteasen, die spezifisch membrangebundene substrate spalten Download PDF

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Publication number
WO2000068416A3
WO2000068416A3 PCT/EP2000/004057 EP0004057W WO0068416A3 WO 2000068416 A3 WO2000068416 A3 WO 2000068416A3 EP 0004057 W EP0004057 W EP 0004057W WO 0068416 A3 WO0068416 A3 WO 0068416A3
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WO
WIPO (PCT)
Prior art keywords
localising
proteases
membrane
specifically connected
split substrates
Prior art date
Application number
PCT/EP2000/004057
Other languages
English (en)
French (fr)
Other versions
WO2000068416A2 (de
Inventor
Christian Haass
Harald Steiner
Brigitte Pesold
Original Assignee
Boehringer Ingelheim Pharma
Christian Haass
Harald Steiner
Brigitte Pesold
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma, Christian Haass, Harald Steiner, Brigitte Pesold filed Critical Boehringer Ingelheim Pharma
Priority to MXPA01010500A priority Critical patent/MXPA01010500A/es
Priority to EP00934985A priority patent/EP1177313A2/de
Priority to JP2000616381A priority patent/JP2002543797A/ja
Priority to CA002370212A priority patent/CA2370212A1/en
Publication of WO2000068416A2 publication Critical patent/WO2000068416A2/de
Publication of WO2000068416A3 publication Critical patent/WO2000068416A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4711—Alzheimer's disease; Amyloid plaque core protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Gegenstand der Erfindung ist ein Verfahren zur Auffindung von Proteasen, die membranassoziierte Substrate spalten sowie die dafür notwendigen rekombinanten Fusionsproteine.
PCT/EP2000/004057 1999-05-05 2000-05-05 Methoden zur auffindung von proteasen, die spezifisch membrangebundene substrate spalten WO2000068416A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
MXPA01010500A MXPA01010500A (es) 1999-05-05 2000-05-05 Metodos para encontrar proteasas que separan especificamente sustratos unidos a membranas.
EP00934985A EP1177313A2 (de) 1999-05-05 2000-05-05 Methoden zur auffindung von proteasen, die spezifisch membrangebundene substrate spalten
JP2000616381A JP2002543797A (ja) 1999-05-05 2000-05-05 膜結合基質を特異的に切断するプロテアーゼを発見する方法
CA002370212A CA2370212A1 (en) 1999-05-05 2000-05-05 Methods of discovering proteases which specifically cleave membrane-bound substrates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19920514.0 1999-05-05
DE1999120514 DE19920514A1 (de) 1999-05-05 1999-05-05 Methoden zur Auffindung von Proteasen, die spezifisch membrangebundene Substrate spalten

Publications (2)

Publication Number Publication Date
WO2000068416A2 WO2000068416A2 (de) 2000-11-16
WO2000068416A3 true WO2000068416A3 (de) 2001-05-31

Family

ID=7906944

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/004057 WO2000068416A2 (de) 1999-05-05 2000-05-05 Methoden zur auffindung von proteasen, die spezifisch membrangebundene substrate spalten

Country Status (6)

Country Link
EP (1) EP1177313A2 (de)
JP (1) JP2002543797A (de)
CA (1) CA2370212A1 (de)
DE (1) DE19920514A1 (de)
MX (1) MXPA01010500A (de)
WO (1) WO2000068416A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001062897A1 (en) * 2000-02-25 2001-08-30 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Presenilin deficient multipotent cell lines and screening methods for intramembrane regulated proteolytic activities using these lines
GB0100119D0 (en) 2001-01-03 2001-02-14 Univ Aberdeen Materials and methods relating to protein aggregation in neurodegenerative disease
WO2003072041A2 (en) * 2002-02-27 2003-09-04 Merck & Co., Inc. Assays to monitor amyloid precursor protein processing
AU2002253468B2 (en) * 2002-04-18 2008-04-03 Esbatech Ag Method for the identification of modulators of a secretase activity
EP1481987A1 (de) * 2003-05-26 2004-12-01 Aventis Pharma Deutschland GmbH Screening-Verfahren für Inhibitoren der gamma-Sekretase-Aktivität
JP2007074940A (ja) * 2005-09-13 2007-03-29 Sankyo Co Ltd プレセニリン活性化に関与するプロテアーゼ、プレセニリネース及びその用途
CN102549006A (zh) * 2009-08-31 2012-07-04 乔治亚大学研究基金公司 植物人工染色体及其制造方法
WO2022259904A1 (ja) * 2021-06-07 2022-12-15 国立研究開発法人理化学研究所 プロテアーゼ活性測定用の融合タンパク質基質

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19641180A1 (de) * 1996-09-24 1998-03-26 Schering Ag Verfahren zur Darstellung von APP-Sekretase Modulation und deren Verwendung als Mittel zur Behandlung der Alzheimer'schen Erkrankung
DE19856261C1 (de) * 1998-12-07 2000-03-30 Hoechst Marion Roussel De Gmbh Aß-Peptid Screening Assay

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19641180A1 (de) * 1996-09-24 1998-03-26 Schering Ag Verfahren zur Darstellung von APP-Sekretase Modulation und deren Verwendung als Mittel zur Behandlung der Alzheimer'schen Erkrankung
DE19856261C1 (de) * 1998-12-07 2000-03-30 Hoechst Marion Roussel De Gmbh Aß-Peptid Screening Assay
WO2000034511A2 (de) * 1998-12-07 2000-06-15 Aventis Pharma Deutschland Gmbh Peptid screening test zum nachweis von ass

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; GUNYUZLU, PAUL L. ET AL: "A yeast genetic assay for caspase cleavage of the amyloid-.beta. precurso protein", XP002149493, retrieved from STN Database accession no. 2000:510076 *
DIXON E P ET AL.: "An Inverse Mammalian Two-Hybrid System for Beta Secretase and Other Proteases", ANALYTICAL BIOCHEMISTRY, vol. 249, 1997, pages 239 - 241, XP000914815 *
MOL. BIOTECHNOL. (MAY 2000), 15(1), 29-37 *
SISODIA, S S: "Beta-amyloid precursor protein cleavage by a membrane-bound protease", PROC. NATL. ACAD. SCI. USA, vol. 89, July 1992 (1992-07-01), pages 6075 - 6079, XP002149492 *
SMITH T A ET AL.: "Direct selection for sequences encoding proteases of known specificity", PROC. NATL. ACAD. SCI. USA, vol. 88, June 1991 (1991-06-01), pages 5159 - 5162, XP000946644 *
STEINER, HARALD ET AL.: "An in vivo assay for the identification of target proteases which cleave membrane-associated substrates", FEBS LETTERS, vol. 463, 17 December 1999 (1999-12-17), pages 245 - 249, XP000946647 *

Also Published As

Publication number Publication date
JP2002543797A (ja) 2002-12-24
MXPA01010500A (es) 2002-06-04
CA2370212A1 (en) 2000-11-16
EP1177313A2 (de) 2002-02-06
WO2000068416A2 (de) 2000-11-16
DE19920514A1 (de) 2000-11-16

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