JPH01502080A - 組み換えビタミンｋ依存性蛋白質のガンマカルボキシル化の増強 - Google Patents

組み換えビタミンｋ依存性蛋白質のガンマカルボキシル化の増強

Info

Publication number
JPH01502080A
JPH01502080A JP50045588A JP50045588A JPH01502080A JP H01502080 A JPH01502080 A JP H01502080A JP 50045588 A JP50045588 A JP 50045588A JP 50045588 A JP50045588 A JP 50045588A JP H01502080 A JPH01502080 A JP H01502080A
Authority
JP
Japan
Prior art keywords
prothrombin
dna sequence
protein
vitamin
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP50045588A
Other languages
English (en)
Japanese (ja)
Inventor
フリー，ブルース・イー
フリー，バーバラ・シー
ジョージェンセン，マリア・ジェイ
Original Assignee
ニュー・イングランド・メディカル・センター
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ニュー・イングランド・メディカル・センター filed Critical ニュー・イングランド・メディカル・センター
Publication of JPH01502080A publication Critical patent/JPH01502080A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP50045588A 1986-11-17 1987-11-17 組み換えビタミンｋ依存性蛋白質のガンマカルボキシル化の増強 Pending JPH01502080A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93165186A 1986-11-17 1986-11-17
US931,651 1986-11-17

Publications (1)

Publication Number Publication Date
JPH01502080A true JPH01502080A (ja) 1989-07-27

Family

ID=25461141

Family Applications (1)

Application Number Title Priority Date Filing Date
JP50045588A Pending JPH01502080A (ja) 1986-11-17 1987-11-17 組み換えビタミンｋ依存性蛋白質のガンマカルボキシル化の増強

Country Status (4)

Country Link
EP (1) EP0289586A4 (fr)
JP (1) JPH01502080A (fr)
AU (1) AU8339187A (fr)
WO (1) WO1988003926A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05211874A (ja) * 1990-06-20 1993-08-24 Teijin Ltd 組換えヒト・オステオカルシン

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5516650A (en) * 1985-06-27 1996-05-14 Zymogenetics, Inc. Production of activated protein C
FR2638643B1 (fr) * 1988-11-09 1991-04-12 Transgene Sa Sequence d'adn codant pour le facteur ix humain ou une proteine analogue, vecteur d'expression, cellules transformees, procede de preparation du facteur ix et produits obtenus correspondants
JP3153236B2 (ja) * 1989-12-22 2001-04-03 ザイモジェネティクス，インコーポレイティド 端が切り取られた軽鎖を有する組換えプロテインｃ
US5225537A (en) * 1989-12-29 1993-07-06 Zymogenetics, Inc. Methods for producing hybrid phospholipid-binding proteins
DK0512011T3 (da) * 1990-01-26 1994-05-09 Immuno Ag Blodfaktorer fremstillet ved rekombinant DNA-teknologi, fremgangsmåde til ekspression af blodfaktorerne samt vacciniavirusrekombinanter anvendt ved fremgangsmåden
CA2044985A1 (fr) * 1990-06-20 1991-12-21 Hiroshi Eguchi Osteocalcine humaine recombinante
US5268275A (en) * 1991-05-08 1993-12-07 The University Of North Carolina At Chapel Hill Vitamin K-dependent carboxylase
EP0611302B1 (fr) * 1991-10-15 2000-03-29 MULLARKEY, Michael F. Recepteurs pour le traitement des troubles inflammatoires de phase tardive
US5618714A (en) * 1993-12-15 1997-04-08 Eli Lilly And Company Methods for producing protein C
US5910481A (en) * 1995-11-13 1999-06-08 Immuno Ag Hybrid proteins with modified activity
ATE321846T1 (de) * 1996-11-08 2006-04-15 Oklahoma Med Res Found Verwendung eines modifizierten protein-c
WO2000054787A1 (fr) * 1999-03-16 2000-09-21 The Children's Hospital Of Philadelphia Gamma carboxylation amelioree de facteurs de coagulation vitamine k dependants
CN1481438A (zh) 2000-10-02 2004-03-10 ŵ��Ų�ڿ˽�������ɷݹ�˾ 制备维生素k-依赖性蛋白的方法
EP2380985B1 (fr) 2003-09-23 2014-01-01 University of North Carolina at Chapel Hill Cellules exprimantes la vitamine K epoxyde réductase et utilisation des-dites.
GB0324044D0 (en) 2003-10-14 2003-11-19 Astrazeneca Ab Protein
JP2008532544A (ja) 2005-03-15 2008-08-21 ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル 活性ビタミンｋ依存性タンパク質を生産するための方法及び組成物
CA2602793C (fr) 2005-04-13 2016-11-22 Astrazeneca Ab Cellule hote a vecteur de production de proteines demandant une gamma-carboxylation
US8206967B2 (en) 2007-07-06 2012-06-26 Medimmune Limited Method for production of recombinant human thrombin
WO2010024704A2 (fr) * 2008-08-27 2010-03-04 Universidade Do Algarve Protéine riche en gammacarboxyglutamate, procédés et tests pour la détecter, purification et quantification et utilisations de celle-ci
US9631002B2 (en) 2010-12-21 2017-04-25 The University Of North Carolina At Chapel Hill Methods and compositions for producing active vitamin K-dependent proteins
JP2014525475A (ja) 2011-09-06 2014-09-29 メディミューン，エルエルシー 凝固因子を処理する方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52140940A (en) * 1976-05-19 1977-11-24 Philips Nv Solar heat collecting apparatus
JPS5318835A (en) * 1976-08-05 1978-02-21 Philips Nv Solar heat collecting apparatus
JPS57192749A (en) * 1981-05-22 1982-11-26 Matsushita Electric Ind Co Ltd Vacuum tube type solar heat collector

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0430930B1 (fr) * 1985-03-15 1993-09-01 Btg International Limited Protéine facteur IX
GR860984B (en) * 1985-04-17 1986-08-18 Zymogenetics Inc Expression of factor vii and ix activities in mammalian cells
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52140940A (en) * 1976-05-19 1977-11-24 Philips Nv Solar heat collecting apparatus
JPS5318835A (en) * 1976-08-05 1978-02-21 Philips Nv Solar heat collecting apparatus
JPS57192749A (en) * 1981-05-22 1982-11-26 Matsushita Electric Ind Co Ltd Vacuum tube type solar heat collector

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05211874A (ja) * 1990-06-20 1993-08-24 Teijin Ltd 組換えヒト・オステオカルシン

Also Published As

Publication number Publication date
AU8339187A (en) 1988-06-16
EP0289586A1 (fr) 1988-11-09
EP0289586A4 (fr) 1990-04-10
WO1988003926A1 (fr) 1988-06-02

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